LOCUS       DAVLYR010000001        80153 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLYR010000001 DAVLYR010000000
VERSION     DAVLYR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 80153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.001"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            490..1008
                     /locus_tag="VYO63_000001"
     CDS             490..1008
                     /locus_tag="VYO63_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1670993.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            1138..1644
                     /locus_tag="VYO63_000002"
     CDS             1138..1644
                     /locus_tag="VYO63_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1670994.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            1644..1814
                     /locus_tag="VYO63_000003"
     CDS             1644..1814
                     /locus_tag="VYO63_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1670995.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            1846..3078
                     /gene="clpX"
                     /locus_tag="VYO63_000004"
     CDS             1846..3078
                     /gene="clpX"
                     /locus_tag="VYO63_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1670996.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            3087..3674
                     /locus_tag="VYO63_000005"
     CDS             3087..3674
                     /locus_tag="VYO63_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1670997.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            3685..4065
                     /locus_tag="VYO63_000006"
     CDS             3685..4065
                     /locus_tag="VYO63_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1670998.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            4117..5007
                     /gene="rapZ"
                     /locus_tag="VYO63_000007"
     CDS             4117..5007
                     /gene="rapZ"
                     /locus_tag="VYO63_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1670999.1"
                     /translation="MTKKQFHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            5004..5981
                     /locus_tag="VYO63_000008"
     CDS             5004..5981
                     /locus_tag="VYO63_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1671000.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            5978..6889
                     /gene="whiA"
                     /locus_tag="VYO63_000009"
     CDS             5978..6889
                     /gene="whiA"
                     /locus_tag="VYO63_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1671001.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(6977..8104)
                     /locus_tag="VYO63_000010"
     CDS             complement(6977..8104)
                     /locus_tag="VYO63_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1671002.1"
                     /translation="MWMEELPNGKFKYFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNLFYKSWAQTVKESTKHNCKSVDKKMKEVIPSD
                     TILANLDRRFLQEAIEKIIESNGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIP
                     QKPKTLEELEKKRNNFLTMQEIKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTAL
                     QLKNIDFENNKIEITGNFDSVNKIKTLPKTTNSIRTIKVSESVIEAIQRQIVRLSERF
                     QPLSSDDYIFCFEKWNQPTTIACFIQILKKYGKQAKIEKNLSSHIFRHSHISFLAESD
                     LPIKSIMDRVGHSNAKMTLEIYSHTTEDMEDKLVNKLDTIF"
     gene            complement(8383..9333)
                     /locus_tag="VYO63_000011"
     CDS             complement(8383..9333)
                     /locus_tag="VYO63_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEV1671003.1"
                     /translation="MKPFKTIEEQIATLKIRGLSITDESKAAKYLLSNNYYNIINGYS
                     KFFQHPGTDTYIDGVTFDEVSSLYTFDKDVKRAILQAILEAEHHIKSITAHRFAEAYP
                     SQKYAYLNTNSYADNKILDVGFIVSKLSKIINTNKRYRGNSIHHYAHTHSDVPIWVLT
                     DYLEFGDLRTIIENLPNSLQNEIARDLVSFISTNIPDFNDVFPPETLISFLKNINEVR
                     NKCAHNNRLLNFRCRSNSTFWETIHNKEILMGDDSRKTVYSTIISLQCFISKAAFNIL
                     WNTLRKKVIKLEKKLPSIDINVINQSLGFPNDWHRNEPKV"
     gene            complement(9482..10273)
                     /locus_tag="VYO63_000012"
     CDS             complement(9482..10273)
                     /locus_tag="VYO63_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1671004.1"
                     /translation="MDEKKRMQIIAENITHFRKQRGITQKELAKEVGITASTMTDYMK
                     LRSAPSFGVIQKLADYFGVKKSDIDTTFKEESTNSLPDAPDSLTQQIMDKVVQLTPPN
                     QKIVLRTSEELLESQNEEETKINEVSEVISLYQVEVVSETAAASGFNYGFGYDDTDRE
                     TIEVDERPPRHDIATKVSGDSMQPDYQDGDILYLVDKGLTTYNGDLAIIAYGDRSYFK
                     KIYTENGRLRLVSLNDKYEDIILDFPPAEDTHIKIYAVVGVYRGE"
     gene            complement(10517..11206)
                     /locus_tag="VYO63_000013"
     CDS             complement(10517..11206)
                     /locus_tag="VYO63_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEV1671005.1"
                     /translation="MIFRVKVNSSVSRPVTVEDICPNCKKPTNPDLVNSSYFSLGENK
                     TSLVLTCRCLGCKHFWTEEFIATRFLINDYTEKYEIEHIKVIPNLPSDIPISDDVEIV
                     SPIGKQIYVQALKAEHEQLDHIAGIGYRKALEFFVKDFSIVTNPDDEDKIIKMSLKQV
                     IEKYIKDEDLKTFALASAYIGNDEGHYYRNNPDKGFTDLKNYLHGVIHYMEMKLNFLD
                     AQELVNRSKKS"
     gene            11413..11577
                     /locus_tag="VYO63_000014"
     CDS             11413..11577
                     /locus_tag="VYO63_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671006.1"
                     /translation="MKENIEHLHERIKHFQSLIAYMSEHEKKYYLEKDWFDNPTLISI
                     EDAKKEVELL"
     gene            11596..11811
                     /locus_tag="VYO63_000015"
     CDS             11596..11811
                     /locus_tag="VYO63_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1671007.1"
                     /translation="MQWTLEAMRINKGLTQAELAEKFEVSSQTIARLEKDSSDIGYQL
                     LKKYMFFFNVKFDDIFLGKKYENFVNN"
     gene            11843..12043
                     /locus_tag="VYO63_000016"
     CDS             11843..12043
                     /locus_tag="VYO63_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671008.1"
                     /translation="MESVLESLKQEKDHLEKIIKVVTSGGKFLRLPYQKKSRSISENL
                     KLISQNLDKLSEQVQQTTNQHS"
     gene            complement(12442..12633)
                     /locus_tag="VYO63_000017"
     CDS             complement(12442..12633)
                     /locus_tag="VYO63_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671009.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            12892..13062
                     /locus_tag="VYO63_000018"
     CDS             12892..13062
                     /locus_tag="VYO63_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671010.1"
                     /translation="MKEAVKEFLKFRSRFTKIEWFEINQAVEARLNQKADQLKLDDVD
                     LEIISSRLEKVI"
     gene            complement(13055..13294)
                     /locus_tag="VYO63_000019"
     CDS             complement(13055..13294)
                     /locus_tag="VYO63_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671011.1"
                     /translation="MTEIHACLCGNWVNLSADDDCVMGPNMASPYIWWEENAELYSPI
                     SKPEANSMYHQDYIYIHYRGADYRIHPMFIQIVSR"
     gene            13412..13684
                     /locus_tag="VYO63_000020"
     CDS             13412..13684
                     /locus_tag="VYO63_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671012.1"
                     /translation="MEAVEIVRIKDVIIEKVSANDEELKRIFGCSKRQAGERRREMQK
                     LPSQQKHLLDSGQLVTIKGFYEYLQYRGTKAWKKEMETSKKMRSAG"
     gene            13681..13896
                     /locus_tag="VYO63_000021"
     CDS             13681..13896
                     /locus_tag="VYO63_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671013.1"
                     /translation="MNLLSRIKNYFSEEVKETNLDWKEVALDLNQSLIETQEKLQEAN
                     QEIADLKKIVAIYKEKEKEKRWNIFTW"
     gene            13875..14063
                     /locus_tag="VYO63_000022"
     CDS             13875..14063
                     /locus_tag="VYO63_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671014.1"
                     /translation="MEYIYLVTIVGIGLWSLVNKLDDHAEMKQKERQLMANNVARMNL
                     RNSDKQFTYDVEPPEGLK"
     gene            14075..14416
                     /locus_tag="VYO63_000023"
     CDS             14075..14416
                     /locus_tag="VYO63_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671015.1"
                     /translation="MTQAERIREYYREHPAASYDEVAEVVGTTNSNVRANLAKDIKAG
                     RCVRLEDKSYDYSPYYNHTQALTELVDWKNDNRREWVDMLTRAAEKETDSNVMRLLIK
                     EANKLMKEVTK"
     gene            14417..14647
                     /locus_tag="VYO63_000024"
     CDS             14417..14647
                     /locus_tag="VYO63_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671016.1"
                     /translation="MVRNKIGDLTNTLFAQLETLDDRDLTADELKVELHRSKQMVAIS
                     GQILQAGQLALDAEKFKDKVGEVNAPIALLEG"
     gene            14661..15383
                     /locus_tag="VYO63_000025"
     CDS             14661..15383
                     /locus_tag="VYO63_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1671017.1"
                     /translation="MHEICPGRLTPEVTRLLNEKFGTNYNKSQIGGVRKRLGLAVGKV
                     YQGRLLTKEQHDYLVLIQKNKISRNVANEMNQKFGLSLTEKQIKSYRRNNNLHSGLTG
                     RFEKGQTPHNKGKKYPNMPKNGGQFKKGNRPPNYVPVGTINYTTNGYPKEKIGEPNQW
                     VLKHRKVWEDHHGLIPKGYSIVFLDGDKTNYDISNLACLSKNEIARMNQNHLFTSNAD
                     LTKSGIGLTKLTNKIREVEKNG"
     gene            15376..15858
                     /locus_tag="VYO63_000026"
     CDS             15376..15858
                     /locus_tag="VYO63_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEV1671018.1"
                     /translation="MASLYELTGQFLTIYQLDIDDETKADTLEAIDWQEQFEQKAEGY
                     AHVIKNLEADVAMYKAEEESFKAKKQVAQKKLDYVKDNIMAAMNVTGQTEVKSGALII
                     KIAKNPESVKVNEDDLPKKYFTKKVTLAPDKKTLKELLKSGKKVKGAELVRTEKLVIK
                     "
     gene            15858..16217
                     /locus_tag="VYO63_000027"
     CDS             15858..16217
                     /locus_tag="VYO63_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671019.1"
                     /translation="MELMNKTRVTDSLAVVIGPESIEVLVTEGFLFDVAIRFVKVDET
                     NLDQGNEKPVFTPEYKLVTVAKYKEKPIFESEEDIRKFEKQAKEVKSLFAFAKVNKQN
                     WFNTALYPGVLTEKVGV"
     gene            16217..16702
                     /locus_tag="VYO63_000028"
     CDS             16217..16702
                     /locus_tag="VYO63_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671020.1"
                     /translation="MKILAIDPSSNKIETSTTGVVLLDNARLVDSWVVSYGMRGFADW
                     FHEIGTNLEFDVVIVEEFKARDNDKSKDNSVAETIAYIQLCYPGAILQFNAGYKSDIP
                     NDLLKILDLWKFEKSHHQDIRAAARLGLFWAMRNDIEEVVHDIGKVVSEYHNNAKKVA
                     S"
     gene            16665..17858
                     /locus_tag="VYO63_000029"
     CDS             16665..17858
                     /locus_tag="VYO63_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEV1671021.1"
                     /translation="MSITITLRKWQAEAIKRSEHLSNGIFLEALGGRGKTICALAIAK
                     HKKAKKIIITNNRLAILNGWIDAVKFMNFDKGVEIIIQTDRYLQNQVKKGHKLDCDVL
                     IVDEWQNMSSDKQVALYRKIKRKYTIGLSATPIRKKGQNFYPLEKTVFGWATPNNKFD
                     WQKTHGKMVYDPFSYSKEKWEDFQNYESYISSLPNFFRWEEIEGIENAVENNGFEIKF
                     YQKRVASGNPEKLAEFRKLNLVTVDGKTAMAKQSFGRKTFERYLNQTGVAVDFPKLKP
                     VNADTPLMLQLDGLIERAPHDMLIVSKSKQIVNVISERHPEIGIWTGDIQEGLYKKFV
                     VATSQVLGVGVDGLQHKYQTIVVLDPVEEGSGEYDDYRQLLWRITGSRQQHDVNVIEF
                     YYKES"
     gene            17876..18193
                     /locus_tag="VYO63_000030"
     CDS             17876..18193
                     /locus_tag="VYO63_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671022.1"
                     /translation="MNKTTEMIVFRSRKTGEFLNSYKDRSSLAFAADFCSLEYCLKLP
                     RKKYEDNKKTYKALAAAFDCEIVAVEAEYKLTYPNGSEVEPIKRDRSSIEDMIKDIIG
                     GVL"
     gene            18193..19020
                     /locus_tag="VYO63_000031"
     CDS             18193..19020
                     /locus_tag="VYO63_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1671023.1"
                     /translation="MAFTLPANKPQVPKDTPRNFFIYGETMSGKSYLANEFPNPIVLN
                     TDGNAEANTVPSIQLINEKDDKGRITNSVIKQLGDILLALQTQKHSYETVVIDVIDDV
                     IEMIKIAVCDELTPVGKPRLKSLSEIPYGKGYDFFNQAITELVIDLKALPMNVIYISR
                     QVSEYDDNGNATKDKPSLKDKYVNLINGNSDLMIHTEKLGNNYNREVDRKRKTYYADQ
                     VDDKAILKILATIRGAVEPAKGKLAPKKEAAKTTKPAKTEKTKEAPKKEVDSDDELF"
     gene            19040..19546
                     /locus_tag="VYO63_000032"
     CDS             19040..19546
                     /locus_tag="VYO63_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671024.1"
                     /translation="MSLLDIAKSIKKEGFDPRKDSANGPAPIPAGTYPVVLKKATFNV
                     SDKGWESLGYQFEIRGGDYSGRSEFATFGTLTEWNGKNLDWAVERTMKFFIKALVLAG
                     DSMQGNEEDGKALEEALQRKAVGSYYNLVISVTKGKDGREFRNYDLEEEEAQPLTEAD
                     IDEDDLPF"
     gene            19719..20540
                     /locus_tag="VYO63_000033"
     CDS             19719..20540
                     /locus_tag="VYO63_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEV1671025.1"
                     /translation="MPSMKEYALQYQKLGFSVIPINPKNKMPLIEFADKPAMTPSEIE
                     NFWDGYPNANIALKTTNFFVIDIDKHGKSNGFESLKKWKHLNLIEPTLQAKTASGGKH
                     LFYFKREDEPITQMIGFLPGVDIKAHENNYVLVAPSATDKGQYEWDLEKSKEGGTMVT
                     PSKYLIQSIKKQYGETHGYKYDGKDGLRDLVRRSHTRDRTQTTDLFETIALGFGDEGG
                     RNDKLAKFVGGLLYRAVDDGVVVQLARLANANSPTPLPEKEMMRTIESMIKKDRR"
     gene            20543..21886
                     /locus_tag="VYO63_000034"
     CDS             20543..21886
                     /locus_tag="VYO63_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1671026.1"
                     /translation="MIGNVVSIDSQPKMITTAKGDIKANSPSNVLMSFKADDQLSIYL
                     KHNDFSQEHELLKDIKIGNTLFKKGELPSNFDSVVKVYFESVLGVAFSSQAMLDGMET
                     FFSERSYNPVIEYMEKAAEKWDGRKRIDRMLQVYLGAEDIPLVSKIAQMWLVGAVAKV
                     YDPYVKFDYVLDLVGGQGVGKTSLLQKLGGEWYTDAVTDFSNKDNYDIMLKSLIVNDD
                     EMVASNRMSFAETKAFISKTSLRYRKPYMKRTEEFAKNFILARTTNQTEYLKDKTGER
                     RFLPVMADSKRQKKHPMEIEPETIEQIWGEAVTIYRAGADLMFDENTEDELNIYREQF
                     MYRDEVELQVLEYLDMPVPENWQNWSIQQQHQYTSKYFDNSSDFDPGSKKLDKVSTRE
                     MMYNLFMRNSNDRKLSTKINMIMDNHPDWKKSVFRAGGKSTKGFVRVKDSEKTNR"
     gene            22179..22334
                     /locus_tag="VYO63_000035"
     CDS             22179..22334
                     /locus_tag="VYO63_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671027.1"
                     /translation="MSYTVTLFFDNMVDETHFFKKESDAAKCKAQLESKYRGNRMYKV
                     KQEKLEE"
     gene            22331..23026
                     /locus_tag="VYO63_000036"
     CDS             22331..23026
                     /locus_tag="VYO63_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1671028.1"
                     /translation="MNKQELIKRIEDLPYTEGPIADTIEINRNWILKSIEQLAESEIG
                     HADEAPRYVKNILARLRELPLHDREFWLKAIMSEFEQDFSHAKWREGYEQGKIEGMVE
                     REKVIVPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLDG
                     YEVEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELENAKIGW
                     VFDCEGFEIEEVE"
     gene            23184..23444
                     /locus_tag="VYO63_000037"
     CDS             23184..23444
                     /locus_tag="VYO63_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV1671029.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYEKVKVGDEVLL"
     gene            23444..23902
                     /locus_tag="VYO63_000038"
     CDS             23444..23902
                     /locus_tag="VYO63_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671030.1"
                     /translation="MDDILQALAKMLNMTVDEVSSLLTTFKGNAPQIYEMFVKEKMFY
                     DLFSLFQIMSIVIFSISAVVLAVLTLIYFTYDGGFVYSYDIRTGKTEEEIKLERIERK
                     RKDLKIPLKISCISSSASLITLVIAIVLKATLAPNYIFIVNEILPKLTKR"
     gene            23950..24018
                     /locus_tag="VYO63_000039"
                     /pseudo
     CDS             23950..24018
                     /locus_tag="VYO63_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            24015..24452
                     /locus_tag="VYO63_000040"
     CDS             24015..24452
                     /locus_tag="VYO63_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671031.1"
                     /translation="MIPKFRVWVKIGKRMVFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIG
                     IVKQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            24449..24820
                     /locus_tag="VYO63_000041"
     CDS             24449..24820
                     /locus_tag="VYO63_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671032.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            24893..25297
                     /locus_tag="VYO63_000042"
     CDS             24893..25297
                     /locus_tag="VYO63_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1671033.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            25378..25460
                     /locus_tag="VYO63_000043"
     tRNA            25378..25460
                     /locus_tag="VYO63_000043"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25409..25411,aa:Tyr,seq:gta)
     gene            25727..26206
                     /locus_tag="VYO63_000044"
     CDS             25727..26206
                     /locus_tag="VYO63_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV1671034.1"
                     /translation="MAFFDYSKGGDGKLSGLRIKQKRFADEYIISGNATEAYKKAGYR
                     VSSDRVAGVEGHKLLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQT
                     LISIGELGQTITDIDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDISD"
     gene            26184..27482
                     /locus_tag="VYO63_000045"
     CDS             26184..27482
                     /locus_tag="VYO63_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1671035.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            27494..28963
                     /locus_tag="VYO63_000046"
     CDS             27494..28963
                     /locus_tag="VYO63_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1671036.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSRLKNLKSYYM
                     GDHAILNHKRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            28872..29903
                     /locus_tag="VYO63_000047"
                     /pseudo
     CDS             28872..29903
                     /locus_tag="VYO63_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            30263..30490
                     /locus_tag="VYO63_000048"
     CDS             30263..30490
                     /locus_tag="VYO63_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HEV1671037.1"
                     /translation="MAILDDLQALYDNGWDASFNYNGQVCGIFPNSIYDVVVIIADDE
                     YRASSFDDLISLQIEGKTLPEIMNEVEVQYG"
     gene            30639..30851
                     /locus_tag="VYO63_000049"
     CDS             30639..30851
                     /locus_tag="VYO63_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671038.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDS
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            31021..31605
                     /locus_tag="VYO63_000050"
     CDS             31021..31605
                     /locus_tag="VYO63_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV1671039.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            31612..32457
                     /locus_tag="VYO63_000051"
     CDS             31612..32457
                     /locus_tag="VYO63_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEV1671040.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTAVAKEWIKGSELGANMVVSGTFGEVDGVQIVRSKKVDEGKGFLV
                     KVSPSQTQTDDANKYGAFVILLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGGA"
     gene            32469..32651
                     /locus_tag="VYO63_000052"
     CDS             32469..32651
                     /locus_tag="VYO63_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEV1671041.1"
                     /translation="MSLLLRRHYIQEEQVNQYSDLENKTLEELKDLAKEAGVAGAYKL
                     TKAEIVEVLEELKSEI"
     gene            32641..32829
                     /locus_tag="VYO63_000053"
     CDS             32641..32829
                     /locus_tag="VYO63_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671042.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVT
                     ISDVLEKILPEPEFLEED"
     gene            32831..33142
                     /locus_tag="VYO63_000054"
     CDS             32831..33142
                     /locus_tag="VYO63_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV1671043.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTDLLQRIRMHRLARVAGHVFEA
                     E"
     gene            33123..33461
                     /locus_tag="VYO63_000055"
     CDS             33123..33461
                     /locus_tag="VYO63_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671044.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            33436..33951
                     /locus_tag="VYO63_000056"
     CDS             33436..33951
                     /locus_tag="VYO63_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1671045.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            33948..34316
                     /locus_tag="VYO63_000057"
     CDS             33948..34316
                     /locus_tag="VYO63_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671046.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGIVDLHSELVFQFRMEN"
     gene            34319..34732
                     /locus_tag="VYO63_000058"
     CDS             34319..34732
                     /locus_tag="VYO63_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1671047.1"
                     /translation="MLANGITLSYGETKDSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKKKLYFEQAYPDGTKVIFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSNLTFTDGIGG"
     gene            34737..35117
                     /locus_tag="VYO63_000059"
     CDS             34737..35117
                     /locus_tag="VYO63_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671048.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVDK"
     gene            35156..35506
                     /locus_tag="VYO63_000060"
     CDS             35156..35506
                     /locus_tag="VYO63_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671049.1"
                     /translation="MFLDIEGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            35493..39146
                     /locus_tag="VYO63_000061"
     CDS             35493..39146
                     /locus_tag="VYO63_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671050.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSSSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIERIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNIGNISEAVGNIAQDFSSTFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPILALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDIFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSWFGN
                     MFTSAYNAVKNAFSSIGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFKSAVNAVLGTIE
                     NVVNDFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGISPQGGFSSDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            39155..39505
                     /locus_tag="VYO63_000062"
     CDS             39155..39505
                     /locus_tag="VYO63_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671051.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSKFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     LKPWSGAKFNLVER"
     gene            39510..45125
                     /locus_tag="VYO63_000063"
     CDS             39510..45125
                     /locus_tag="VYO63_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV1671052.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGEYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMTTGRSLELDNVFITQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFIFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGFEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEALSRTKNELDGASTLLAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEA
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASRIASVQA
                     SGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGYNALKIIGLNPSGRDGGNPK
                     VTYPVLGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIK
                     RYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQI
                     STVESTFKQRVNSLEAGVNRLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQ
                     KLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKT
                     VGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTFSAWIKYENVVQG
                     RNFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITFTYDYSEKTNFDQ
                     LKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDGENELLVAKTEFKRTADGL
                     STKMAAVESYVGQDGQRQEALQRYTREESTRQATAVRELVNRDFVGKATYQEDVKGIN
                     QRIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSE
                     QGTTTQISNISNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQVSANKANAD
                     SQFANVTNQLARKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEV
                     GKYSVSGPNLIKNSDFKNATNEWGSTQNLGRLVKHSFYHNGQKDLMRLSNATKNENFL
                     YSHRFNLERNTDYVLNFRGFNNSALASYDVYILGRRAGESDGFTIVKKVVSSKKLSTS
                     RCEDVSVTFNSGEMDNAYIRFDNNGSSSGTADLYITEVDLYKGYKPRTWQPHPEDAVA
                     DANKKLEATQTKMTQLAGSWVVENINSAGDIISGINLGANGHNRFVGKLTHITGETLI
                     DRAVIKSAMVDKLKTGNFEAGSVTTTILDAEAVTAEKLKVDDALIRKLTANDAFIDRL
                     TSKRIFSTKVESVISSSTFLEAYQGRIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAG
                     HGVRTAFWANWGNNWNYAGPKAWNVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTT
                     FSRSPVFSNGIELGSKDVLGDGWNPKGGRNAVVWWNQVGSGSVKYWMEQKSDRRLKEN
                     ITDTAVKALDKINRLRMVAFDFIENKKHEEIGLIAQEAETIVPRIVSRDPENPDGYLH
                     IDYTALVPYLIKAIQELNQKIEKMEKTIA"
     gene            45220..45423
                     /locus_tag="VYO63_000064"
     CDS             45220..45423
                     /locus_tag="VYO63_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671053.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITNNILSIQLDEQREGNQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            45426..45776
                     /locus_tag="VYO63_000065"
     CDS             45426..45776
                     /locus_tag="VYO63_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671054.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            45786..46085
                     /locus_tag="VYO63_000066"
     CDS             45786..46085
                     /locus_tag="VYO63_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671055.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            46089..46421
                     /locus_tag="VYO63_000067"
     CDS             46089..46421
                     /locus_tag="VYO63_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1671056.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            46425..47381
                     /locus_tag="VYO63_000068"
     CDS             46425..47381
                     /locus_tag="VYO63_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1671057.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLRAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKNTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(47633..48601)
                     /locus_tag="VYO63_000069"
     CDS             complement(47633..48601)
                     /locus_tag="VYO63_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1671058.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFVPSQLLGDGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            48737..49594
                     /locus_tag="VYO63_000070"
     CDS             48737..49594
                     /locus_tag="VYO63_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1671059.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            49581..50360
                     /locus_tag="VYO63_000071"
     CDS             49581..50360
                     /locus_tag="VYO63_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1671060.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            50384..51736
                     /locus_tag="VYO63_000072"
     CDS             50384..51736
                     /locus_tag="VYO63_000072"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1671061.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            51810..52184
                     /locus_tag="VYO63_000073"
     CDS             51810..52184
                     /locus_tag="VYO63_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1671062.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            52186..53034
                     /locus_tag="VYO63_000074"
     CDS             52186..53034
                     /locus_tag="VYO63_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1671063.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASEFPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            53311..54078
                     /locus_tag="VYO63_000075"
     CDS             53311..54078
                     /locus_tag="VYO63_000075"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1671064.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            54090..55274
                     /locus_tag="VYO63_000076"
     CDS             54090..55274
                     /locus_tag="VYO63_000076"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1671065.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVIKETYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            55271..57142
                     /locus_tag="VYO63_000077"
     CDS             55271..57142
                     /locus_tag="VYO63_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1671066.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLTTLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(57285..58469)
                     /gene="mntE"
                     /locus_tag="VYO63_000078"
     CDS             complement(57285..58469)
                     /gene="mntE"
                     /locus_tag="VYO63_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1671067.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            58773..61469
                     /locus_tag="VYO63_000079"
     CDS             58773..61469
                     /locus_tag="VYO63_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1671068.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            61482..62273
                     /gene="yghU"
                     /locus_tag="VYO63_000080"
     CDS             61482..62273
                     /gene="yghU"
                     /locus_tag="VYO63_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1671069.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTESVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            62389..62799
                     /locus_tag="VYO63_000081"
     CDS             62389..62799
                     /locus_tag="VYO63_000081"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1671070.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            62811..64406
                     /locus_tag="VYO63_000082"
     CDS             62811..64406
                     /locus_tag="VYO63_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671071.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            64473..64637
                     /locus_tag="VYO63_000083"
     CDS             64473..64637
                     /locus_tag="VYO63_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1671072.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            64716..65444
                     /gene="yaaA"
                     /locus_tag="VYO63_000084"
     CDS             64716..65444
                     /gene="yaaA"
                     /locus_tag="VYO63_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1671073.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(65721..66269)
                     /locus_tag="VYO63_000085"
     CDS             complement(65721..66269)
                     /locus_tag="VYO63_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1671074.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            66590..67078
                     /locus_tag="VYO63_000086"
     CDS             66590..67078
                     /locus_tag="VYO63_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1671075.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            67075..68262
                     /locus_tag="VYO63_000087"
     CDS             67075..68262
                     /locus_tag="VYO63_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1671076.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            68255..68620
                     /locus_tag="VYO63_000088"
     CDS             68255..68620
                     /locus_tag="VYO63_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1671077.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            68636..69982
                     /gene="asnS"
                     /locus_tag="VYO63_000089"
     CDS             68636..69982
                     /gene="asnS"
                     /locus_tag="VYO63_000089"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1671078.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVKNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            70135..70425
                     /locus_tag="VYO63_000090"
     CDS             70135..70425
                     /locus_tag="VYO63_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1671079.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            70437..70907
                     /gene="ssbA"
                     /locus_tag="VYO63_000091"
     CDS             70437..70907
                     /gene="ssbA"
                     /locus_tag="VYO63_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1671080.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            70939..71178
                     /locus_tag="VYO63_000092"
     CDS             70939..71178
                     /locus_tag="VYO63_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1671081.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            71473..72873
                     /locus_tag="VYO63_000093"
     CDS             71473..72873
                     /locus_tag="VYO63_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1671082.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSIRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            72875..73234
                     /locus_tag="VYO63_000094"
     CDS             72875..73234
                     /locus_tag="VYO63_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1671083.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEKKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            73288..74037
                     /locus_tag="VYO63_000095"
     CDS             73288..74037
                     /locus_tag="VYO63_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1671084.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            74027..74326
                     /locus_tag="VYO63_000096"
     CDS             74027..74326
                     /locus_tag="VYO63_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1671085.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            74397..75332
                     /locus_tag="VYO63_000097"
     CDS             74397..75332
                     /locus_tag="VYO63_000097"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1671086.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRIITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            75418..75930
                     /locus_tag="VYO63_000098"
     CDS             75418..75930
                     /locus_tag="VYO63_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1671087.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERDLHDSDF"
     gene            75955..76563
                     /locus_tag="VYO63_000099"
     CDS             75955..76563
                     /locus_tag="VYO63_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1671088.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            76664..76858
                     /locus_tag="VYO63_000100"
     CDS             76664..76858
                     /locus_tag="VYO63_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1671089.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(76904..78349)
                     /locus_tag="VYO63_000101"
     CDS             complement(76904..78349)
                     /locus_tag="VYO63_000101"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1671090.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMEKSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            78455..80077
                     /locus_tag="VYO63_000102"
     CDS             78455..80077
                     /locus_tag="VYO63_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1671091.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
BASE COUNT        24765 a        14600 c        18291 g        22497 t
ORIGIN      
        1 tttgaaagag ggatgtgata gtagcaccct ctttttcttt gttttatgat agttcatcct
       61 cgagtaaatt caagatcttg ctcggaaatg aagcttatat agtagattga atatagacaa
      121 agaccttgtg attggtaaaa cattttagaa attcatttac ctttcctaat cgacttggtt
      181 tcatcttatt tcaatctatt atagtattgg ggaatttctt caaaccacat cagcttggtc
      241 agttctacct gcgacctcaa aacttgtgct ttggtcaagc tgggtttagt ttcctagttt
      301 gctgatggat ttccattgac tataagcatc caaccctctt ttttgtcttc taaagaattc
      361 ttaaattatc agtctattgc aacttttctc atataagttc tttgtcttgc tattggtttt
      421 ccttagtagt atactaaggt agtaatcatt aagaagtggt tacaaaaaat aatgaatgag
      481 gtaaagaaaa tggtagaatt gaaaaaagaa gcagtaaaag acgtaacatc attgacaaaa
      541 gcagcgccag tagcattggc aaaaacaaag gaagtcttga accaagctgt tgctgatttg
      601 tatgtagctc acgttgcttt gcaccaagtg cactggtata tgcatggtcg tggtttcctt
      661 gtatggcatc caaaaatgga tgagtacatg gaagctcttg acggtcaatt ggatgaaatc
      721 agcgaacgct tgattacact cggtggaagc ccattctcta cattgacaga gttccttcaa
      781 aatagtgaaa tcgaagaaga agctggtgaa taccgtaatg ttgaagaaag cttggaacgt
      841 gttcttgtta tctaccgtta cttgtcagaa cttttccaaa aaggtttgga tgtcactgat
      901 gaagaaggtg acgatgtaac aaacggtatc tttgcaggcg ctaaaactga aacagataaa
      961 acaatttgga tgcttgcagc cgaacttgga caagcacctg gtttgtaaga aataaggaaa
     1021 catagataaa tctgtaggaa atttcttaca gattttttct attttgtaag ctattccaaa
     1081 ccagtctttt tttgagtttt ttgataaaat agtactatca gtgaaaagga tggaagcatg
     1141 actaagaaaa tcgtagctat ttgggcccag gatgaagagg gtgtgattgg taaggaaaat
     1201 cgtctgcctt ggcatttgcc agcagaattg cagcacttta aagaaacaac tctgaatcat
     1261 gctatcttga tggggcgtgt gacctttgat gggatggggc gtcgcttgct tccaaaacgg
     1321 gaaaccctga ttttgacgcg taatccggaa gaaaagatag atggggttgc tacttttcag
     1381 gacgtccagt ctgttcttga ctggtatcag gatcaagaaa agaatctcta cattatcggt
     1441 gggaagcaaa tttttcaggc ttttgaacct taccttgatg aagtgattgt cactcacatt
     1501 catgctcggg tggaaggaga tacctatttc cctgaagagc ttgacttgtc tctttttgag
     1561 acagtttcaa gcaaatttta cgccaaagat gagaagaatc cttatgattt taccatccaa
     1621 taccgcaaga gaaaggaagt ctaatggaac ggagtttatt tggtctattc acggcttttc
     1681 tgtgtttcat ctgctttctg gcaggagcac aggcttttcg taaaaagcgt tacgggcttt
     1741 ctatcctcct ttggttaaat gcctttacca atctggtcaa tagtattcat gctttttaca
     1801 tgaccttatt ttagatagaa tgacagtata gaatggaagg aaatcatgtc tacaaataga
     1861 aaaaatgata tgatggttta ttgctcattt tgtggcaaaa accaagaaga agtacaaaaa
     1921 ataattgctg gcaacaatgc ttttatttgt aatgaatgcg tggagttagc tcaggaaatc
     1981 attcgagaag aattggttga ggaagtcttg gcagacttgt ctgaggtgcc aaaaccaatt
     2041 gaactcctcc atatcttgaa ccactatgta attggtcaag atcgtgccaa gcgtgccttg
     2101 gcagtggcgg tttataacca ctacaaacgc atcaatttcc acgatacacg cgaagagtca
     2161 gaagatgtgg atttgcagaa gtcaaacatt ttgatgattg gcccaactgg ttcagggaaa
     2221 actttccttg cccagacctt ggctaagagc ttgaatgtgc cttttgctat tgcggatgcg
     2281 acagctctga cggaggctgg ttatgtgggt gaggacgtgg aaaatatcct cctcaaactc
     2341 ttgcaggttg ctgactttaa catcgaacgt gcagagcgtg gcattatcta tgtggatgaa
     2401 attgacaaga ttgccaagaa gagtgagaat gtgtctatca cacgtgatgt ttctggtgaa
     2461 ggggtgcaac aagcccttct caagattatc gagggaactg ttgctagcgt gccgcctcaa
     2521 ggtggacgca aacatccaca acaagagatg attcaagtgg atacaaaaaa tatcctcttc
     2581 atcgtgggtg gtgcttttga tggtattgaa gaaattgtca aacaacgtct gggtgaaaaa
     2641 gtcatcggat ttggtcaaaa taataaggcg attgacgaaa acagctcata tatgcaagaa
     2701 atcatcgctg aagacattca aaaatttggt attatccctg agttgattgg acgcttgcct
     2761 gtttttgcgg ctcttgagca attgaccgtt gatgacttgg ttcgcatctt gaaagagcca
     2821 agaaatgcct tggtgaaaca ataccaaact ttgctttctt atgatgatgt tgagttggaa
     2881 tttgacgacg aagcccttca agagattgct aataaagcaa tcgaacggaa gacaggggcg
     2941 cgtggacttc gctccattat cgaagaaacc atgctagatg ttatgtttga ggtgccgagt
     3001 caggaaaatg tgaaattggt tcgcatcact aaagaaactg tcgatggaac ggataaaccg
     3061 atcctagaaa cagcctagag gtgactatgg aacttaatac acacaatgct gaaatcttgc
     3121 tcagtgcagc taataagtcc cactatccgc aggatgaact gccagagatt gccctagcag
     3181 ggcgttcaaa tgttggtaaa tccagcttta tcaacactat gttgaaccgt aagaatctcg
     3241 cccgtacatc aggaaaacct ggtaaaaccc agctcctgaa cttttttaac attgatgaca
     3301 agatgcgctt tgtggatgtg cctggttatg gctatgctcg tgtttctaaa aaggaacgtg
     3361 aaaagtgggg gtgcatgatt gaggagtact taacgactcg ggaaaatctc cgtgcggttg
     3421 tcagtctagt tgaccttcgt catgacccgt cagcagatga tgtgcagatg tacgaatttc
     3481 tcaagtatta tgagattcca gtcatcattg tggcgaccaa ggcggacaag attcctcgtg
     3541 gtaaatggaa caagcatgaa tcagcaatca aaaagaaatt aaactttgac ccgagtgacg
     3601 atttcatcct cttttcatct gtcagcaagg cagggatgga tgaggcttgg gatgcaatct
     3661 tagaaaaatt gtgaggaaaa gaaaatggca aaaacaattc atacagataa ggccccaaag
     3721 gctatcggac cctatgttca aggaaaaatc gttggcaacc ttttgtttgc tagcggtcaa
     3781 gttcccctat cccctgaaac tggggaaatt gtaggagaga atatccaaga acagacagag
     3841 caagtcttga aaaacatcgg tgctattttg gcagaagcag gaacagactt tgaccatgtt
     3901 gtcaaaacaa cttgcttctt gagcgatatg aacgactttg ttccttttaa tgaggtttac
     3961 caaacggcct tcaaagagga attcccagct cgttcagctg tggaggtagc tcgtcttcct
     4021 cgtgatgtaa aagtcgaaat tgaagtcatc gcagagattg gataagctag ttgaagtttg
     4081 gtgttgccaa acttcttttg atataaggag aaaaagatga caaagaaaca atttcacttg
     4141 gtgattgtga cagggatgag tggcgcaggg aaaactgtag ccattcagtc cttcgaggat
     4201 ctaggttatt tcaccattga taatatgccg ccagctctct tgcctaagtt tttgcagctg
     4261 gttgaaatta aggaagacaa tcctaagttg gccttggtag tggatatgcg tagccgttct
     4321 ttcttttcag agattcaagc tgttttggat gagttggaaa atcaagatgg tttggatttc
     4381 aaaatcctct ttttggatgc ggctgataag gaattggtcg ctcgttacaa ggaaaccaga
     4441 cggagtcacc cactagcagc agacggtcgt attttagatg gaatcaagtt ggaacgtgaa
     4501 ctcttggcac ctttgaaaaa tatgagccaa aatgtggtgg atacgactga actcactcca
     4561 cgtgagctgc gcaaaaccct tgcagagcag ttttcagacc aagaacaagc ccagtctttc
     4621 cgtatcgaag tcatgtcttt cggatttaag tatggaatcc cgattgatgc ggacttggtc
     4681 tttgatgtcc gtttcttgcc aaatccctat tatctaccag aactgagaaa ccaaacgggt
     4741 gtggatgaac ctgtttatga ttatgtcatg aaccatcctg agtcagaaga cttttatcaa
     4801 catttattgg ccttgattga gccgattctg ccaagttacc aaaaggaagg taagtccgtt
     4861 ttgaccattg ccatgggatg tacgggtgga caacaccgta gtgtggcatt tgctaaacgc
     4921 ttggcgcagg acttatccaa gaattggtct gttaatgaag ggcatcgcga caaagaccgc
     4981 agaaaggaaa cggtaaaccg ttcatgagaa aaccaaagat aacggtgatt ggtggaggga
     5041 ctggaattcc cgtcattcta aaaagtctgc gggaaaaaga tgtggaaatc gcagctatcg
     5101 tgacggtggc agatgatggt ggttcttcag gtgaactccg aaaaaatatg caacagttga
     5161 caccgccagg tgatcttcgt aatgtccttg tggccatgtc ggatatgcct aagttttatg
     5221 agaaggtctt tcagtatcgg ttctctgagg atgccggagc ctttgctggc catccattgg
     5281 gaaatctcat cattgctggc ttgtcagaaa tgcagggttc aacctataat gccatgcagt
     5341 tattgagcaa atttttccat acaacaggga aaatttatcc ttccagtgac catcctttga
     5401 cccttcatgc agtctttcag gatgggacag aagtggctgg agagagtcat attgtagacc
     5461 atcgaggcat aattgacaat gtctatgtga ccaatgccct aaacgatgat acgcctctgg
     5521 ccagccgtcg agtagtgcag accatccttg aaagtgacat gattgtccta gggccaggtt
     5581 ccctctttac ctctattttg cccaatatcg tgattaagga aattgggcgg gctcttttgg
     5641 aaactaaggc agaaattgcc tatgtctgca atatcatgac ccaacgtggg gagacggaac
     5701 actttacaga tagcgaccac gtggaagtct tgcatcgtca ccttggtcgc ccttttatcg
     5761 acactgtctt ggtgaatatt gaaaaagtgc ctcaggaata catgaattcc aaccgttttg
     5821 atgaatactt ggtgcaagtg gaacacgatt ttgtaggtct ttgtaagcaa gtttcgcgcg
     5881 tgatttcatc taacttcctt cgtctggaaa atggcggtgc cttccacgat ggagatttga
     5941 ttgtggacga gttgatgcgc attatacagg tgaaaaaatg agtttcacag tagcagtaaa
     6001 agaagaaatc ctgggccaac accatctgag ctggcatgaa ttatctgcca ttatcaaaat
     6061 gtctggtagc atcggtctct cgacttcggg cttgactttg tcagttgtga cagaaaatgc
     6121 caaactggcc cgtcacctct atgagtcctt tctccatttc tatgaaatca aatctgaaat
     6181 tcgtcaccac caacggagca atcttcgcaa gaatcgcgtc tataccgttt ttacagatga
     6241 aaaggtgcag gatttgttaa gtgatttgca cttggcagac tctttctttg gcctggaaac
     6301 aggtattgat gaggcgattt tatcggatga ggaagcaggt cgtgcctatc tctgtggcgc
     6361 tttcttggca aatgggagca ttcgtgaccc tgagtcaggc aagtaccagt tggaaatcag
     6421 ttctgtttat ctggaccacg cgcaaggaat tgcctccctt ctccaacagt ttttactgga
     6481 tgccaaggtg cttgagcgca agaagggggc tgtgacctat ctccagcgag cagaagacat
     6541 tatggacttc ttgatagtaa taggagccat gcaggcacgt gatgattttg agcgggttaa
     6601 gattttgcga gaaacccgta acgacctcaa tcgggctaat aatgccgaga cagctaatat
     6661 cgctcggaca gtttctgcca gcatgaagac cattaacaat atcagtaaaa tcaaagatat
     6721 catgggctta gaaaatttgc cagtagattt gcaggaagtg gcgcaactga ggattcagca
     6781 cccagactac tctatccagc agttagcaga tagtctcagc acccctctga ccaaaagtgg
     6841 tgtcaatcac agactcagaa aaatcaataa gatagcggat gaattataac gatataaagc
     6901 aaccccttga attatcaata tcttcaaggg gttttgttgt gttatgaaat caaaaagggg
     6961 caatcaaggg gcagaattaa aaaatagtat ctaatttatt gaccagttta tcctccatat
     7021 cctcagtagt atgagaatag atttccaaag tcatttttgc atttgagtgc ccaactcgat
     7081 ccattattga ttttattggg aggtcagact ctgctaaaaa cgaaatatga gaatgcctaa
     7141 aaatatggct agataagttt ttttctattt tggcctgttt tccatatttt tttaatatct
     7201 gtatgaagca agctattgtt gtaggttgat tccatttttc aaaacagaaa atataatcat
     7261 cgcttgacaa tggctggaaa cgttcgctaa gtcgtactat ttgtctttga atagcttcta
     7321 tgacactctc tgatactttg attgtccgta ttgaatttgt agtctttggt agcgtcttga
     7381 ttttgtttac tgaatcaaaa ttacctgtga tctcaatttt gttgttttcg aagtctatat
     7441 tcttcagttg taaggcagtt aactcaccat atctcatacc agttaatgtc agcacaagaa
     7501 ccatatcagc gtacttttgg tgatattctc gacgattaag gacatcgaca agtgctttta
     7561 tttcttgcat ggtgagaaag ttgttacgct ttttttccag ttcttctaaa gtctttggtt
     7621 tttgaggaat cgtagtataa tcgacctcgt tgttttcaat gtaagagtat tgaacagcgt
     7681 aattaaagat acctctgagc ctatgccgta cttttttagc tgtaatatat ccgttgcttt
     7741 caataatttt ttcaatagcc tcttgaagaa aacgcctgtc aagattagca agtatggtat
     7801 cggatggtat gacttccttc atcttcttat caactgattt acaattatgt tttgttgatt
     7861 cctttactgt ttgcgcccat gatttataga aaaggttata gatttcttca aatgtaatgc
     7921 tttctacttg ttttgtgctg agttttttat ttatcttctc ttgtaacaag atagcagctt
     7981 gatttcttgc ctggggagtt ttcttctcca tggttactga aacttttttt aatttctcag
     8041 tatatggatc tttatatcgc tcaaaatatt taaatttgcc atttggcaat tcttccatcc
     8101 acattgcgtt tacctcactt ttttgttaaa atgggtacaa gaaaactacc tttttaatgg
     8161 ttgtttccta tacatgattt cctcacactc tccttggcca aaatttgagt gtggggattt
     8221 tttggttgct atctcgtcag ataataaaat aaggtgtacc tcttagaagt acaccagacg
     8281 attttatcct tgacgaacaa ggctttttta cattcttagt gtatttcttt ttttaatttc
     8341 tgtcaagcag ttatttccta aatggaaata gttggtttta atttatactt ttggttcatt
     8401 acggtgccaa tcattaggaa aacctaaaga ctggttgatt acattgatgt ctatagaagg
     8461 cagctttttc tctagtttga taactttttt tctaagagta ttccacaaaa tgttgaatgc
     8521 tgctttacta ataaagcatt gaaggctaat aattgttgaa tatacagttt ttctactgtc
     8581 atctcccatc aagatctctt tgttatgaat cgtttcccaa aacgtactat tagaacggca
     8641 tctgaagttt aataagcgat tattgtgagc gcatttgttt cgtacctcgt taatattttt
     8701 tagaaaagat atcaaggttt ctggtgggaa aacatcgtta aagtcaggta tgtttgtgct
     8761 tataaaactt actaaatccc gtgcgatttc attttggagt gaattaggca ggttttcaat
     8821 aatcgtgcgt aaatctccaa actctagata atcagttagt acccagattg ggacatctga
     8881 atgagtatgt gcgtagtggt gaattgaatt tcctctgtat cgcttgtttg tgtttatgat
     8941 tttggatagt tttgatacaa taaatcccac gtctaatatt ttattatctg cataagaatt
     9001 ggtgtttaaa taagcgtatt tttgactggg gtaagcttcg gcgaatctgt gagcagtgat
     9061 tgacttgata tggtgttcag cttcaagaat agcttgcaaa atggctcgtt ttacatcttt
     9121 gtcaaatgta tagagacttg aaacttcgtc aaatgtgacg ccgtcaatat aagtgtcagt
     9181 accagggtgt tgaaaaaact tactgtatcc attgataata ttgtagtaat tgttacttag
     9241 caggtactta gctgctttag attcatctgt aatggataac cctctgattt ttagtgttgc
     9301 gatttgttcc tcgattgttt taaatggctt caaaaaaact cccccattct aatttagaat
     9361 gaaggagttt ccccgtatcg gtccccgtag agatcccgac gctttttctc taatgaattc
     9421 attctaaatg atttttttaa aaaagtcaac tgttttttaa tatttttaaa aaataatttt
     9481 tttattcccc tctatacacc ccgactactg cataaatctt gatgtgtgtg tcttcggctg
     9541 gtgggaagtc taggatgatg tcttcgtact tgtcattgag tgacactagg cgtaagcgtc
     9601 cgttttcggt atatatcttc ttgaagtaag aacggtctcc atatgcgata attgctaaat
     9661 ctccgttgta ggtagtcagt cctttgtcta ctaaatagag aatgtctccg tcttggtagt
     9721 cgggttgcat ggagtctcca ctgaccttgg tcgcaatatc gtggcgtggt ggtcgctcgt
     9781 caacctctat agtctctctg tctgtatcgt cgtacccaaa tccatagtta aatccagaag
     9841 ctgctgccgt ctcagataca acctcaactt ggtacaagct gataacttcc gatacttcgt
     9901 ttatcttcgt ttcttcttcg ttttggctct ccagaagctc ctcagaggtc cgtagcacga
     9961 ttttttgatt gggtggggtt aattgcacta ccttatccat tatctgctgt gtgagcgaat
    10021 ctggagcgtc tggcagtgag ttggtggatt cttctttaaa agtagtatct atatctgatt
    10081 ttttaacacc gaaataatca gccagttttt ggataacacc aaaagaagga gcgcttctta
    10141 acttcatata gtctgtcata gtacttgctg taattccaac ttctttagcc aactcttttt
    10201 gggtgatgcc acgttgcttt ctaaagtgtg taatattttc agcaataatt tgcattcgtt
    10261 ttttttcgtc catttacgat tacctcgtat tttttataat tacattatat atcatttttg
    10321 aatgataaac aatcaaaaat acgaaaaaat cctatttttt tgataaaaac tattgacaat
    10381 acgaaaaaat cgtattataa ttaaatcaag cttaaggaaa aggaggtaag gcaaatgaac
    10441 gaactagaaa gaacagccct caatgagata ttgaggactg tgacatatat agctgagaag
    10501 ttggatgaag tagattctaa gatttctttg aacgattcac aagctcttga gcatcaagga
    10561 aattgagttt catttccatg tagtgaataa ctccgtgtag gtagttcttt aggtctgtaa
    10621 aacctttatc aggattattt ctatagtaat gaccttcgtc gttaccgata taagcagatg
    10681 caagcgcaaa tgttttaagg tcttcatcct tgatatattt ttcaataact tgttttaatg
    10741 acattttaat gattttatct tcatcatcag gatttgtaac aatggagaaa tctttaacaa
    10801 agaactcaag cgccttacgg tatccaatac ctgcaatgtg gtctagttgt tcgtgttcag
    10861 ctttcagtgc ttggacatag atttgcttac caattggaga aactatctct acatcgtcag
    10921 atataggtat atcgctcgga agattaggga ttactttgat atgttcaatt tcgtattttt
    10981 cggtatagtc attgattaaa aatcttgtag ctataaactc ctctgtccaa aagtgcttac
    11041 aacctaagca tctacatgtt aatactaagc ttgttttatt ttctccaaga gaaaaataag
    11101 aagagttcac aagatctgga ttggttggtt ttttacaatt tgggcaaatg tcttcaacgg
    11161 ttacaggcct agaaacagaa gaatttactt tcactcgaaa tatcataaga tttctccaat
    11221 cgttttattt tgattatacc atatttgaaa gggggtgagg aaatgagacc aagacgatat
    11281 ccgtatagtg ggaaaaaaga gtccaccttc gtaaaggcag accctgagtt aatgttgaat
    11341 atcaataaga ttgatgttgg aaatatacaa gcgaaaaata tatttggaaa aatttaagga
    11401 gtaggaggat ggatgaaaga aaatatagaa catcttcatg aacgcataaa gcattttcag
    11461 tcattgattg cttatatgtc tgagcatgag aaaaagtatt atttagaaaa agattggttc
    11521 gataacccaa ccctaatttc tatagaagac gcaaaaaaag aagtagaact actttaacag
    11581 aaaggaggta ggaacgtgca gtggacttta gaagctatga gaatcaacaa aggacttact
    11641 caagcagagt tggcagaaaa atttgaagtt tcaagtcaaa caattgctag attagaaaaa
    11701 gatagctctg atatcggtta tcagctattg aaaaaataca tgtttttttt caatgtgaaa
    11761 ttcgatgata tttttttagg gaaaaaatac gaaaatttcg taaataacta gaaacaaaaa
    11821 aataagaagg aggaacacat agatggaaag cgttcttgaa agcttgaaac aagaaaaaga
    11881 ccacctcgaa aaaatcatta aggtagttac ctctggtggt aaatttctga gattgccgta
    11941 tcaaaaaaag tcacgctcga ttagtgagaa tctgaaattg atttctcaaa atcttgataa
    12001 attgagcgag caagttcaac aaaccacgaa tcagcattca tgatttcaag atgacgaaag
    12061 aaacctgttt tgatttccag tttgatatca tgttacagcg actcgataaa gcgattagtg
    12121 attaaatttt tgagatgtaa gtttttttca tgtcaaactt tttcatgtca aattttttac
    12181 actaataaat attaactaac aacaagtatt aaaagacaac cagtcctact tctctaaata
    12241 aaagagaggg tagaaaaaat aaatacaaag gaggtgagga aatgagacca agacgatatc
    12301 cgtatagtgg gaaaaaagag tccacctttg taaaggcaga ccctgagtta gttgaaaaac
    12361 ttttaagaaa cactagtttt cttgagcgtt tacaaaaaaa gcctatcaat tttcagatag
    12421 actcagaaga atttaagcgt cttagctatg aagccattca tgatacttct caagtaactc
    12481 aatagtagtt attacagaag tcaaaccact gaccttcccc agttgcaatc cgtctgtatg
    12541 gtcaatctgt ttagtagctt cattagcttt agcagagata gcttgcatat cttcagctgt
    12601 taaagattct cgaaaatctt taaaggattt cataaaaact cctcctttct attggaattt
    12661 tgactaaaac ggtgagaggt cctagtcgag attattatag caatttagga ggatattaca
    12721 tcggtcttga ggctgatttt tggaggcaat attggaagat aaaatcatag aacttgctga
    12781 ttacttcatc agcgaatcta caacgtacag agaagcaaaa atagcgtgtg agaagctatt
    12841 gaaacaagtc agccacgaga ttgaactcag ggcgatggaa agtaggacag tatgaaagaa
    12901 gcagtaaagg aatttctaaa attcaggagc cgatttacaa aaatagaatg gtttgaaatc
    12961 aaccaagctg tcgaagctcg tttaaatcaa aaagccgacc agttgaaact ggacgacgta
    13021 gatttagaaa tcatttctag cagactagaa aaagttatct agaaacgatt tgaatgaaca
    13081 ttggatggat acgatagtca gcgccacgat agtgaatgta gatataatcc tgatggtaca
    13141 tcgagtttgc ttcaggttta gaaattggtg agtagagttc tgcattttct tcccaccaaa
    13201 tgtaaggact agccatattt ggtcccatta cacaatcgtc gtcggctgat aggttcaccc
    13261 aatttccgca aagacatgcg tgaatttcag tcataatatt acctcctttc tggtttcatt
    13321 atagcagaaa aggagatagc aaaaaagcac ctgacggaaa tcaggcgcat acttaaatat
    13381 tcaacatgat tataacacga aaggagcaaa aatggaagca gttgaaattg taagaattaa
    13441 agatgtgatt attgaaaaag tctctgctaa tgatgaagag ttaaaacgta tctttggatg
    13501 ttcaaaacga caagcaggag agcgaagaag agaaatgcaa aaactcccta gtcagcaaaa
    13561 acatcttttg gatagtggac aacttgtaac gattaaaggt ttctatgaat acttgcaata
    13621 tcgtggaact aaagcttgga aaaaagaaat ggaaacaagc aagaaaatga ggtcagcagg
    13681 atgaacctac tatcaagaat caaaaactat ttttcggaag aggtcaaaga aactaatctc
    13741 gactggaaag aggtcgcttt agacctcaat caatcactaa ttgaaacaca ggaaaaactt
    13801 caagaagcga atcaagaaat cgcagacttg aagaaaatcg tagcaatcta taaagaaaag
    13861 gagaaagaaa aacgatggaa tatatttacc tggtaacaat cgtaggaatt ggactatggt
    13921 cgctagtaaa taaactagat gaccacgctg aaatgaaaca aaaagagcgc cagctgatgg
    13981 caaacaatgt cgcacggatg aatctgagaa attcagataa gcaatttact tatgatgtag
    14041 aaccgcctga agggttgaaa taaggaggag aaacatgact caagctgaac gaattaggga
    14101 atattataga gagcaccctg ctgcctcata tgatgaagtg gctgaggtcg ttggtacaac
    14161 aaatagtaat gtgagagcga acctggccaa agacatcaag gcaggcagat gcgttcgctt
    14221 ggaagataag tcatacgatt actcgcctta ctataaccat acacaggcac tcactgagtt
    14281 ggttgactgg aagaatgaca acagacgtga gtgggtggat atgctgacaa gagcagcaga
    14341 gaaagaaacg gatagcaacg ttatgcgttt gttaatcaaa gaagcaaata aattgatgaa
    14401 agaggtgact aagtagatgg tacgaaataa aataggtgat ttaactaaca cgctcttcgc
    14461 tcaattagag actctggatg atagggatct tacagcagat gaattaaaag tagaattaca
    14521 ccgctcgaaa caaatggtcg caatctcagg tcaaatctta caagcaggtc aattggcgct
    14581 agatgctgaa aaattcaaag acaaggtagg tgaagtcaat gccccgatcg ctttgctgga
    14641 aggatgagta cacggagtac atgcatgaaa tatgccctgg tcgtttaact cctgaagtaa
    14701 ccaggttgct gaatgaaaaa tttggtacga attataacaa gagtcaaatc ggtggcgtac
    14761 gcaaacgtct agggttagca gttggaaaag tctatcaagg tcgattgctg acaaaggagc
    14821 aacatgatta tcttgtattg atccaaaaaa ataagatttc tcgtaatgtc gcaaatgaaa
    14881 tgaaccaaaa atttggctta tcgctaactg agaaacagat taagagttat cggagaaata
    14941 ataatctaca tagtggtttg acaggaagat tcgagaaagg tcagactcct cacaataagg
    15001 ggaagaagta ccccaatatg ccaaaaaacg gcgggcagtt caaaaaaggt aatcgacctc
    15061 cgaattatgt acctgtcggt actatcaact acacaacaaa cggttatcca aaagaaaaga
    15121 ttggagaacc taatcaatgg gttttgaaac accgcaaggt ttgggaggac catcacgggc
    15181 tgataccaaa agggtactca atcgtttttc tggacggtga taaaacaaac tatgatattt
    15241 caaatctggc atgtttatct aagaacgaaa ttgctagaat gaatcaaaat catttattca
    15301 cgtccaacgc tgatttgact aaatcaggta ttggactaac aaaacttaca aataaaatca
    15361 gagaggtaga aaaaaatggc tagtttatac gaactaacag gtcagttcct gacaatttac
    15421 caattggata tcgatgacga aacaaaagca gacacgcttg aggccatcga ttggcaagaa
    15481 caatttgaac agaaagcaga aggatatgcc catgttatca agaatctaga agccgacgtg
    15541 gcaatgtaca aggctgagga agagagcttc aaagccaaga aacaggtggc acagaaaaag
    15601 ctggattatg tcaaggataa cattatggca gctatgaatg tcacggggca aaccgaagtt
    15661 aagagtggtg ccctgattat aaaaattgct aagaatccag aatcagtcaa ggtcaacgaa
    15721 gacgaccttc cgaaaaaata ttttacaaaa aaagtgacgc ttgcgccgga caaaaaaaca
    15781 ctcaaagagt tgcttaaatc tggcaagaaa gtcaaaggtg cggagcttgt ccggacagaa
    15841 aagttggtga ttaagtaatg gaattgatga ataaaacacg agtaacagat tcactagcag
    15901 ttgtgattgg accagaatcg attgaagtac ttgttactga aggttttcta tttgatgttg
    15961 cgattcgttt tgtaaaagta gacgaaacaa atcttgatca aggaaatgaa aagccagtat
    16021 tcactccgga atacaagctg gtcacagttg ctaaatacaa ggaaaaacct atctttgaat
    16081 cggaggaaga tattcgaaaa tttgagaagc aagcaaaaga agttaaatcg ctatttgcct
    16141 ttgcaaaggt aaataaacaa aattggttta acactgccct ttatccagga gtgctgactg
    16201 agaaagttgg tgtttgatga aaattttagc tattgatcca agtagtaata aaattgaaac
    16261 cagcacaaca ggagttgtct tgttggataa tgcaagatta gttgatagct gggttgtctc
    16321 ttatggtatg agaggtttcg ctgattggtt tcacgaaatc ggaacaaatc ttgaattcga
    16381 tgtagttatt gttgaagaat ttaaggcgag ggataacgac aagtcgaaag ataatagcgt
    16441 ggcagaaacc atcgcctata tccaactttg ctatccaggt gccattcttc aattcaatgc
    16501 aggttacaag tcggatattc caaacgatct tttgaaaatc ttagaccttt ggaaatttga
    16561 aaaaagtcat catcaagata ttcgagcagc agcaagactt ggattatttt gggcaatgag
    16621 aaatgatatt gaagaagtgg ttcatgatat cggaaaggtg gtgagtgagt atcacaataa
    16681 cgctaagaaa gtggcaagct gaagcgatta aaagaagtga acatttatct aatggaatct
    16741 ttttagaggc tcttgggggc agaggcaaaa ctatctgtgc acttgctatt gcaaaacata
    16801 aaaaagctaa aaaaatcatc atcacaaaca atcgactagc tattctgaat ggttggatag
    16861 atgcagtcaa gtttatgaat tttgataaag gtgttgagat tatcattcag acagatagat
    16921 atcttcaaaa tcaagtcaaa aaggggcata aattagattg tgatgtgctg atagtagacg
    16981 aatggcagaa tatgtcttct gacaaacaag tggccttata tcgcaaaata aagcgaaaat
    17041 acacgatagg tctttcagcg acaccaattc ggaaaaaagg acaaaatttc tatccgcttg
    17101 aaaaaacggt atttggttgg gcaaccccaa ataataaatt tgactggcaa aagactcatg
    17161 gaaaaatggt ctatgatcca tttagctatt caaaagagaa gtgggaagat tttcaaaatt
    17221 atgaaagtta tatctcgagc ttgcctaatt tcttccgctg ggaagagatt gaaggaattg
    17281 agaatgcagt tgagaataac ggttttgaga ttaagtttta ccaaaagaga gtcgcctctg
    17341 gcaatccaga aaaacttgca gaatttagaa aactaaatct tgtaacagtg gacggcaaaa
    17401 ctgcaatggc caagcaatcg tttggaagaa agacctttga acgctacctt aatcaaacag
    17461 gcgtagcagt cgattttcca aaattaaagc cagtaaatgc ggatacgcca ttgatgttac
    17521 aacttgacgg tttaatcgaa cgagcaccac acgatatgtt gattgtcagt aaatctaagc
    17581 agattgtcaa cgtcattagc gagcgccatc ctgaaattgg aatctggacg ggcgatattc
    17641 aagaaggact ttataagaaa ttcgtggttg ctactagtca agtgttaggt gtcggagtag
    17701 acggcttgca acacaaatac caaactattg tcgtattgga tccagtagaa gaaggttctg
    17761 gagaatatga tgattatcga caattgctct ggcgcataac aggaagtcgt cagcagcatg
    17821 atgtaaatgt aattgaattt tattataaag aaagttaaaa aaagaggaaa acaaaatgaa
    17881 taaaacaact gaaatgatcg tatttcgtag ccgtaaaact ggagaatttc ttaattctta
    17941 caaggacaga agttctttag catttgcagc tgacttttgc agcttggaat attgtttgaa
    18001 gcttcctcgt aaaaaatacg aagacaacaa aaagacttac aaggctcttg ctgcagcttt
    18061 tgactgtgaa attgtcgcag ttgaagcgga atacaaattg acctatccga atggatcaga
    18121 agttgaacct atcaagcgtg accgttcatc aattgaggac atgattaagg atattattgg
    18181 aggggttctc taatggcatt tacacttcca gcaaataaac cacaagttcc taaagatacc
    18241 ccacgaaatt ttttcatcta cggcgaaacc atgagcggaa agtcttatct tgcaaatgaa
    18301 ttcccaaatc caatcgtttt gaacacagac gggaatgcag aagctaacac tgttccaagc
    18361 attcagctga tcaatgaaaa agatgacaag ggacgaatta ccaattcagt aattaagcag
    18421 cttggagata tcttgcttgc tctccagaca cagaagcact cttatgaaac agtcgttatt
    18481 gatgtaattg acgatgttat tgaaatgatt aagattgcag tttgtgatga attaacccca
    18541 gttggtaaac ctcgcttgaa atccttgtcg gaaattccat acggcaaagg atacgacttc
    18601 tttaaccaag ctatcacaga attagtcatt gacctcaaag cattgccaat gaatgttatt
    18661 tacatcagcc gtcaggtatc tgaatatgat gacaatggca atgccaccaa agacaagcca
    18721 agcttgaaag ataagtatgt caatcttatc aatggaaact ctgatttgat gatccacact
    18781 gaaaaactcg gcaacaacta caaccgtgag gttgaccgca agcgtaagac ctattatgcg
    18841 gaccaggttg atgacaaggc catcttgaaa atcttggcaa ctatccgtgg ggctgttgag
    18901 cctgcaaagg gcaagctagc ccctaaaaaa gaagcagcta agacaactaa accagctaag
    18961 accgaaaaaa caaaagaggc acctaagaaa gaagttgact ctgatgatga actattttaa
    19021 gaaataaagg agaatacaca tgagcttact agatatcgca aaatcaatca aaaaagaggg
    19081 ctttgaccca cgcaaagaca gcgccaacgg tcctgcacca atcccagctg gtacttatcc
    19141 agtagtcctg aagaaagcaa ccttcaacgt atcggacaaa ggctgggaaa gccttggtta
    19201 tcaatttgaa atccgtggcg gtgattacag tggacgctct gaatttgcaa catttggcac
    19261 actgactgaa tggaacggta agaaccttga ctgggcagtt gaacgcacta tgaaattctt
    19321 tatcaaagcc ttggtccttg ctggcgacag tatgcaagga aatgaagaag acggtaaagc
    19381 cttggaagag gctctacaac gtaaggcagt tggctcttac tacaaccttg ttatctctgt
    19441 gactaagggg aaagatggcc gtgagttccg aaactatgac cttgaagaag aagaagcaca
    19501 accgctgact gaagctgata tcgatgaaga tgacctccct ttctaaaaaa tagcaagttt
    19561 tgggtcattg atgaaactga tgaaagatta ggaccattca atacatttga agaggcttat
    19621 caatcgttgt tattttattt aaaaatgact gaagatgaat atcagtcaaa ctatacggcc
    19681 caggaacttg tttatattta caaagaggag aaaaaaccat gccgtcgatg aaagaatacg
    19741 cattacagta ccaaaagtta gggttctcag tcattccaat caatcctaaa aacaagatgc
    19801 ctttaatcga atttgctgat aaaccagcca tgactccatc tgagattgaa aacttttggg
    19861 acggctaccc taatgcaaac attgccctaa agactaccaa cttctttgtc attgatattg
    19921 acaaacacgg caaatcgaac ggttttgaat cgctaaaaaa atggaaacat ctaaatttaa
    19981 tcgaaccgac actgcaagct aagacggcta gtggcggtaa acatctattc tacttcaaac
    20041 gagaagatga gccgatcact cagatgattg gattcttgcc tggtgttgat attaaggctc
    20101 acgaaaataa ttatgtgtta gtcgcaccct ctgccacaga taaagggcag tatgagtggg
    20161 atctggaaaa gtctaaggaa ggtggcacga tggtcactcc ttcaaaatat ttaatccagt
    20221 ctataaaaaa acagtatggc gaaactcacg gttataagta tgatggtaag gacggtctta
    20281 gggatttagt tagacgttca catactagag accgaacaca gactacagat ctctttgaaa
    20341 ccatcgccct tggttttggt gatgaaggtg gacgaaatga caaactagca aaattcgtag
    20401 gtggtctctt atatcgtgcg gtcgacgatg gtgtagttgt tcaacttgca agattagcaa
    20461 atgcaaatag tccaacccct ttgcctgaaa aggaaatgat gcgtactatt gaaagtatga
    20521 ttaaaaaaga taggaggtga ttgtgattgg taatgtagta agtattgact cacaacctaa
    20581 gatgataacg actgccaagg gagacatcaa ggccaacagt ccaagtaatg tgttgatgtc
    20641 tttcaaagct gatgatcagt tgagtattta cctaaagcac aacgattttt cccaagagca
    20701 tgaactcctt aaagatatca agatcggcaa cactcttttt aaaaaaggtg agctcccttc
    20761 taactttgat tcagtcgtaa aagtttactt tgaaagtgtg ttaggtgttg ctttctctag
    20821 ccaggcgatg ctggacggca tggaaacttt tttctcagaa cgttcttaca atccagtcat
    20881 tgagtatatg gaaaaggctg ctgaaaaatg ggatggtcgt aaacgaattg accgaatgct
    20941 tcaggtttac ctgggggctg aagatatccc tttagtttct aaaatcgctc aaatgtggct
    21001 agttggtgca gttgctaaag tttatgatcc atacgttaag tttgactatg ttctggatct
    21061 agtcggtgga caaggagttg ggaaaacatc cctccttcaa aaattgggtg gcgaatggta
    21121 tacggatgcc gtaacagatt tctctaataa agataattac gacattatgt taaagagtct
    21181 aatcgtcaac gacgacgaaa tggtggccag taatcggatg agctttgcag aaactaaggc
    21241 ctttatttct aaaactagcc tacgttatcg taaaccatac atgaaacgca cagaagagtt
    21301 tgccaaaaac ttcattttag ctagaactac taaccaaaca gaatacctca aggacaaaac
    21361 cggtgaacgt cgttttctac cagttatggc agatagtaaa cggcaaaaaa aacatccaat
    21421 ggaaatcgag ccagagacaa ttgaacaaat ctggggcgaa gccgttacaa tctatcgtgc
    21481 tggtgctgat ttgatgtttg atgaaaatac agaggatgaa ctgaatatct accgtgaaca
    21541 gttcatgtat cgtgatgaag ttgaattaca agtgcttgaa tatcttgata tgcccgtccc
    21601 tgaaaattgg caaaactggt ctattcagca acaacatcaa tacacaagta aatatttcga
    21661 taatagtagc gactttgatc ctggaagcaa aaaactagat aaggtctcaa ctcgtgaaat
    21721 gatgtacaac ttatttatga gaaattcgaa tgacaggaag ctgtcaacga agattaacat
    21781 gatcatggat aatcatcctg attggaaaaa aagtgttttc cgggcaggag gtaaaagtac
    21841 aaaagggttc gtaagagtga aagattcgga aaaaactaat cggtagcaat taaaaaatta
    21901 tcggtagtca tcggtagcag ttgaggggga gatcggtagc attctaccga taaaatagga
    21961 catcggtagc acatcggtag cagtctaacc ccttgatatt actgactttt atttaatatt
    22021 tatatataat gctactcttc tacctatatt tttaaaaaaa gtatataaaa taatagtaat
    22081 aataaagaaa gcctatagaa tagggattct tgaaaaaaac tttttacttt ttagaattta
    22141 tcggtagcac ggtagcagtt tagaaaaaag aggtaaaaat gtcatacaca gtaacattat
    22201 tttttgacaa catggtagac gaaactcact tttttaagaa agagagtgat gctgccaaat
    22261 gcaaggctca gttagaaagc aagtatcgag ggaatcgaat gtataaagtt aagcaggaga
    22321 aattggaaga atgaataagc aggaactgat taaacgtatc gaggatttgc cttatacaga
    22381 ggggcctatc gcagatacaa tcgaaattaa tagaaattgg atattgaaat caattgaaca
    22441 gctagccgaa tccgaaatag gtcacgcaga tgaagctcca cgctacgtaa agaacatact
    22501 agcacgattg cgagaattgc cattgcatga tagagagttt tggttaaagg ctatcatgag
    22561 cgaatttgaa caggatttta gccatgcaaa atggcgagag ggctacgagc aaggtaaaat
    22621 tgagggtatg gttgaacgtg aaaaagtcat agttccgcag tgtgtggcgg aatatataga
    22681 atttaaaaag aaaaacaatt ttcatgttta cggtgcaatg agagtaattg aagatcatta
    22741 tgataagaaa gttcctgatt ggttttacga aaataacatc gaaaaattct gtcttgcttg
    22801 gcttgacggc tacgaggttg aaaaagagaa gcggtatttt gttaagatta aagggaatat
    22861 taaagaaaat atgttggttt atggagaact tttgaaaagg tatttcttta caaaaagctt
    22921 tagtttagac gatgttatat attcccacac ccgtaaagaa ctagaaaatg caaaaatcgg
    22981 ctgggtgttt gattgtgaag ggtttgagat tgaggaggtg gagtgatgag ccttacgcta
    23041 aatagcacaa ttggagactt agttttggca atcggagaaa ttatcgttgg ttctgatggt
    23101 aaaaccacta cagcgatact ggagatacct gatcaaagct tttacttaga gattgagctt
    23161 aaattgaagg aggaggtcat aaattgaaac gattcatagc tatctggatt ctgctatctg
    23221 ctggattgaa tatctggcag agtatctaca ttaaaaagtt agaagaaaag cgccctattg
    23281 tcgtctataa agctgataac gcaggcgctg agatattcgg taaagtcgtc gagaaaggac
    23341 ggcatggcaa gctatacacg cttaccattc gtgactacgg ggtgttcgtg gttacgaagg
    23401 acgtgtatga gaaagtgaaa gtaggggatg aggtgttact ctaatggatg atattttaca
    23461 agctttagca aaaatgctaa atatgactgt tgatgaagta agttctttgc ttacaacatt
    23521 taaagggaat gcaccacaga tttatgaaat gttcgttaaa gaaaagatgt tttatgatct
    23581 cttcagtctt tttcaaatca tgtcaattgt aatatttagt atttctgcag tagttttagc
    23641 agttttaact ctcatatatt ttacatacga tggtggtttt gtttattcct atgatatacg
    23701 tacaggaaaa accgaggaag aaattaaatt agaacgcatt gaacggaaaa gaaaggactt
    23761 aaaaatacca ctaaaaatta gttgcatttc atcaagcgca agtttgataa cattagttat
    23821 tgcaattgtt ttaaaagcaa ctcttgcacc taattatata ttcatcgtga atgagatttt
    23881 accaaaatta acgaagagat aggagttatc atgaacgcac tagaaaaagt cgaacaatgg
    23941 tttatcgacc gtgaaatcaa agaccgaaaa ggtcgctgga ttgatggctc gtttgtcaaa
    24001 gaggaggatt tggcatgata ccgaaattta gagtgtgggt aaaaatagga aaacgtatgg
    24061 ttttttcaga tgacattctt gctattgact acgaaaacaa agaaatagtg acacaacaag
    24121 tttattttga gaatggttta ccagacgata gagatatcta ttgttatgat tttgacgaaa
    24181 tcgaactcat gcaatcaaca ggactcaaag acaagaacgg caaggaaatc tttgaaggcg
    24241 atattgtacg aactactaga tttttgggta gagctgacga aattggcggt ttctatgagt
    24301 atgacaagga atttataggg attgttaagc agcttgaggg ttcttgggta attgatacgg
    24361 gcagtgacgc agtatgttta tggactgaaa ttgaagaaaa tgaaatcatc ggcaacatct
    24421 atgaaaataa ggagtttgga ggacgcaaat gagaccttgt aaatatccat attcaggaag
    24481 aagaaaaaaa caagaaacgc cgtcgccaat attttctgca cgaccaattt ttgacgaagt
    24541 tccaattgta gaagaagtta aggttgagtt cggagttgaa gctagtatgg ggcgcatata
    24601 tccagaaacg ttaatacatt tagatatttc tggatacgga aatagagtgc attcagtaca
    24661 tcgcttcccc ggtattttac tgagtgttgg tgagtcaatc caactaaaga tgcttttcta
    24721 taaaagactt agaaatttta ctacagatcg tttcttgacg tttagagaat ctgattggaa
    24781 gttctttatc cgggacctgg tcaacgaatt taagcattaa aaaaagccaa gacactctct
    24841 gcctcagcta atagttctcg caaagactat tatatcacaa aggagacaga gagtgaacaa
    24901 ggctaaagag ctcttgaaag agttgcagga tctggacatg gacatccaaa gccgtataga
    24961 cgaaattaac gagcttgagg caggtttgct ctcaagccct aaatggtccg aggttaaagt
    25021 ccaaggtgga cagactagaa aagttgatga tgtctatact cagttggtag tgatgaaaga
    25081 ggctatagag caggatacta aagaggtcat taacagaaaa cttgaattag gtagaatgat
    25141 caataggctt aaaaatccaa agtacagggc agtattaaga atgacttaca tcaacaaagg
    25201 caccgctgat agcgtttgtt atgatttgaa tatgagtcgt acaacctact acaggttaaa
    25261 aaatgaggcg gtcttagctt tggaagaagt tatctaacct catagtgagc ttatgggact
    25321 ttttggaaca gcacggttct aaaaatctgt tagaatggta gtatcaagaa ttgaaaagag
    25381 aggtctcaga attggtagat ggttacctgt aatgtcaggg ggctgtaatg gccttggagg
    25441 ttcaagtcct cccctctcct ttgagtgttt gtgtcccaga atgagttaaa tcttctgggt
    25501 ggggattcac atatcactca ttaactccta tcactcatta acttaaaaat ggttgcggaa
    25561 gcgactggac ctcgcatgat tgcgtagcta attatattcc ggataagtta taagctagag
    25621 ggtttgattc cctcagaggt tttaaagact acaaaaaata aaaaagaagt caaaatttaa
    25681 tacgcacgca aggtagtagt cgccttgcaa gaaggtcgca catcgtgtgg ctttttttga
    25741 ttattcgaag ggtggtgatg gaaaattgag tggattgaga ataaaacaaa agagatttgc
    25801 agatgagtac atcatctcag gtaatgcgac ggaagcctat aagaaagcag gttatcgtgt
    25861 ttctagtgat agagtggcag gcgttgaagg acataagtta ctaaagaatc ctaagattaa
    25921 aagctatata gatgaacgac tgaaacagct tgattctgaa aagattgcgg atcagcaaga
    25981 ggtcttaggt tatctaactt cagtcatgcg aggagagacg caagaacaga ccttgataag
    26041 cattggagaa ctagggcaga cgattacgga tatagatgta ggagctaaag atagaatcaa
    26101 ggcggctgaa cttcttggta aacggcatag gctttggacg gataaggtag aggcggatgt
    26161 ttctggaacg gtggtgtttg caaatgagtc agacatatca gattaaacaa aacgatattg
    26221 tcgttgactt acctaagaca gtaggagctg gatacggaca gttctggcgc tcaagaagtc
    26281 tttatcgtgt agtcaaaggg tcccgtggtt cgaagaagtc caagacaacc gctttgaatt
    26341 atgttatccg tcttttgaag tatccctggg ccaacttgct tgttattcgt agatactcga
    26401 ataccaacaa gcaatcaact tatacggatt ttaaatgggc gtgtaatgtg ttgggtgtga
    26461 ctcatttgtt taaatttaac gagtctttgc ctgaaataac cataaaagcg actggtcaaa
    26521 aaatcctatt ccgtggtttg gatgatgaac tcaaaattac atctattaca gtcgatatcg
    26581 gtagtctgtg ttgggcatgg ttcgaggaag catatcaaat tgagactgaa gacaagttca
    26641 gcacggttgt tgagtctatc cgtggtagct tagatgtacc tgatttcttt aaacaaatca
    26701 cagtcacatt taacccgtgg aatgagaggc attggctcaa gcgtgtattc ttcgatgaag
    26761 agacgagacg ggctgacaca ttcgctacta caaccactta taaatgcaat gagtggcttg
    26821 atgaagtcga tatcaaacgc tatgaggatt tgtatcatac gaaccccaga cgtgctagaa
    26881 tcgtttgtga tggcgaatgg ggagttgctg aaggtttaat ctacgaaaac gtgactgtca
    26941 aggatttcaa taaagatgaa ttactacaag attcagctaa taaattatgt atcggtcttg
    27001 actttggttt tactcatgat ccaactgctt tgtgttgttc gttgataaat gacacgacga
    27061 aagagattta tgtctttgat gaggcgtata aagtcggatt gataaccaaa gaagttgcga
    27121 agatgataaa agacaaaggt tatcatcgct cacaaatcat tgctgatagc gcagagtcac
    27181 ggctgattga agagctcagg tcagaacatg gcatatctag aataaaagag agtcggaaag
    27241 gtaaggatag tattatggca ggcgtatcca aattgcaagg atacgctatt tatgtgcatc
    27301 cagattgtaa aaacatcatg gatgaatttt atagttactg ctaccagcga gataaagaag
    27361 gcaactggtt gaataaacca gaggataaaa acaaccactt gatggacgct ttgcgttaca
    27421 gccttcaatg tatcgaaggt ggaaaagcaa ccgtccgcag acgttctgat tatggtctat
    27481 agagaggaaa gacatgtacc aatatttaac ctatccacgg gatggatatg atgagggttc
    27541 tttgaagaaa gacctgattt acaaattgat aacgatacat aacactgaaa gctcacgctt
    27601 gaagaattta aaaagctact acatgggtga tcatgctatt ttaaatcaca agagacgcaa
    27661 cgtgaacgca cccaattaca agacggtagc taatcatgcc aaggatatcg cagacacggc
    27721 tacgggctat tttatgggca atcctatcaa gtataacaat actgctgacg gtgatattga
    27781 tgaactactt acagcctttg atggtgctga gattgaccaa gtggatgcgc agaacgcact
    27841 aaatatggct atctacggcc gtgcttacga gtacatctat gctaaagagg gtatgactga
    27901 gttggattca actagtattg atccggagaa tactttcatg gtctacgatg atagtattga
    27961 gcggaagcct ttgtttgcgg tctattacta tgaagtaaaa gacgatacga aagacactac
    28021 caagcaccag gctgaggtct ttaccgaaaa tctgcactat cacatggtgc tgagaagtac
    28081 agattcagga acaactcaga gcgaggaggc aacacctcac aaccttggtc aaatcccaat
    28141 tatcgagtat cgcaataatc actttgcgat tggcgactac gagcaacaaa ttagcttgat
    28201 agacgcttat aattccttga tggggaatcg tgtcaatgat aaggaacagg ctgtagagtc
    28261 tatccttgtc ttgtatggca cgcagttagc agacactcca gaagacgcta aggtagcaat
    28321 gaagattctt tctgaagaag gtcttttgga attgccgggc gatagtgcaa gggctgagtt
    28381 cttgaagaat acgctggacg aaagtgctac tgaaatcttg cgtacagctc ttaaagagga
    28441 catctacaca tttagccatg tgcctaattt gactgatgag aatttcgcag ggaatacatc
    28501 aggcgtagcc atggaattta agctgatggg ccttgagatg attactaaga ccaaggaagc
    28561 gaactataag cgaggattgc gtcagcgtat tgcgattttt gctcattact taggcatgaa
    28621 gcagattgct ttagagtctc attcaatcgt tccacaattc agtcgtggtt tgcctaagaa
    28681 cttattagaa atctctcaga ttgtgaacaa tttggaaggc aaagtgacca atagacagct
    28741 tatttctctc ttgccgtttg tggaagaccc tgacgctgag ctggaagcct tggaagaaga
    28801 gaaaaagaag aacatggaag acatgccgat gttcaacaaa gacaacacga aacccgaaga
    28861 cgaggtagag gatgaagaat caggagtatt gggcgaagag gaaagccaat ctgatttacc
    28921 agcagatgga caaggccgaa aagcaggcag accagttcga taaggtctat caggaagcta
    28981 agacttactt ggataaggaa atcaataaga ttttcgataa attccaacgt gattatggtc
    29041 taagtcaggt agaagctaga caagtcttga agaacatgaa agacaagaaa aatctgaatg
    29101 aacttcgtaa agtacttgaa gcgagaccga atgacccgaa catccaaaga ttactagctg
    29161 acttagacag cccagcttat tctttccgca tgaagcgcct agagcgtttg agcgacgatt
    29221 tagatcgtat gcgtgaatct atctatcatt cggagaagac aggctcagat gccttttata
    29281 gcgacctgat gaaggatagt tactacaagg ctacctttga cctacaacag cagacaggac
    29341 tagcatatgg cttttctggg cttcctgaga acgagattaa acatctacag tcttttagtt
    29401 gggtaggaga tggaagtacg tactcaacag acatctggaa gaatacagga aaacttactt
    29461 ccagcataaa agatgaactc ctcattagcc tcatgacagg cagagataca cgagaaactg
    29521 cacaagcaat tgctgagagg ttcaatgtag gtcagaacga tgcaaggcgt ttggttcgaa
    29581 cagaatccgc cttttttcat aaccagatgg agctactcag ctatgaagaa gcagacatag
    29641 aaaagtatat ctttgtggcc gtcttagaca agcgtacatc acgcatttgt caggagcatg
    29701 acaatcaggt ctatgatagg gataaggctg tccctggtgt caattgtccg cctatgcacc
    29761 cttggtgtag gtctactact gtcggatacg atgaggacgc agactacagc aagttgaagc
    29821 gcagagcaag gaatccagag acaggtaaag ttgagtacgt gcctgccgat atgacttata
    29881 aagagtggta tagcaagtat gtggatggta atagagagtc tattaaacgt aaagcgtttg
    29941 ataaaactat taaagatggt ataatagtaa gtgtatcagg gactacaatt ggacacactc
    30001 cgcctggcaa aataggtttg cctaatagtg tagttcagca taatgctaca aacggagatg
    30061 tccttggtag aacttactat gatgctagag gttttaaaac gaaagatgtt cattttacaa
    30121 accataaaca accggcacgt catccttatg gaaaaatcgg agaacatgct catgattttg
    30181 tatttgatga tgaaggtaag ttcgttagta ggagtactag ggaattaaca gacgatgaaa
    30241 gaaaggagaa tcaagatata ttatggcgat attagatgat ttacaagcgt tatatgataa
    30301 tggatgggac gcttctttta attataatgg tcaagtatgt ggcattttcc ctaattctat
    30361 ttatgatgtt gttgttatta ttgcggacga cgaatataga gcatcttctt ttgacgattt
    30421 gatttcttta cagattgaag ggaaaacttt accggaaatc atgaacgaag ttgaagtaca
    30481 atatggctaa agcacctaga gagatctaag tgctttttta gtacccagaa aggagactat
    30541 ccgtacgatt ttcttgattg tgaatttgaa gtaaaaagaa tttcagtgat agcttgcgat
    30601 actatcctta tagaagccta aaagaaagga acagaaaaat ggaagaatgg aaagaaagat
    30661 ttaaaaaaga atactacgaa ttgaaagaac gattccagaa gttagatatg atgattggga
    30721 aatacgaaaa agggcaacta gagtttgaat ctaaatgtcc gattgattcg ttaaaaggtc
    30781 agcgttcaac catgtggaat tatttaagaa ttctagaaca acgtgcaaaa attgaagaaa
    30841 ttaaactata aaaattaacc gcatcgaaat cgatgcggtt ttcctatgcc ctaaccgtat
    30901 ggaatcccgt acggttttta tattgtccaa actgtaccga tgacaataaa agctgtgctg
    30961 ttccgtcgcc ggacgtaaaa cgagattatc gagtggcgac gtaatcgctg gaggacaatt
    31021 atgtcagaag aaatcaatgc aactgtatct actgaatcaa ctgagactgt cgacactcaa
    31081 ggaaatgttg attcagtgca ggaagaaaag cacgaacgaa ctttcactcg tgctgaaatc
    31141 ggtaagatgc tatctgccga acgctctaaa tgggaagctg agcaagaagc caaggaaaac
    31201 gaagctaaga aacttgccaa gatgaacgct gacgagaaac aaaaatatca gttggatcag
    31261 cgtgagcaag aactagctga ccgtgagaag gctattgctc gcaaggaatt gaccgcagaa
    31321 gctaaagcaa tgctaagtga acgtgactta cctgttgagt tagtaaatgt agttgatttg
    31381 acaagcgcag agacggtatc gcagtctgtc gctgtattgc agaaatcatg ggagcaagcc
    31441 gtgcaaaaag gcgttcaaga aaaactaaaa ggcggagctc caatgaaaca agcgccagtc
    31501 gatagtgacg gtatcacaaa agaagaattt gctcgtatgg gttatcagag tcgaaatgag
    31561 ctctatcaaa agaacccaga gctttataag aaattgaaag gataatagaa aatgacagca
    31621 ggacaaacta aattagccac tatggttaat ccagaagtaa tggcggatat ggtagccgct
    31681 aaattaccta aattgattaa atttacaccg ctagcgtatg tagagacaaa gcttgaaggt
    31741 caaccaggta gcactttaac agtgccagca tgggagtatg caggagacgc tactgaaatt
    31801 gaagaaggcc aagcaattac gccagaccaa ttgactacta aaaagactac tatgaccatc
    31861 aaaaaagcag gtaaaggtta tgaaattacc gatgagtctc ttttgtcagg tcttggtgac
    31921 ccactaggtc aagcgactta ccagcttggt ttagctattg ccaacaagat cgataatgat
    31981 ttggtagcgg tagctaaaac tgcaacacaa catattacag aaactccaac aactcttgag
    32041 gcaatcgata aagctctaga tatctttgag gacgaagaag atgcacagta tgttgctatc
    32101 atcaacccta aagatgctac taagctaaaa actgcagtag caaaagaatg gattaaaggt
    32161 tcagagcttg gagcaaatat ggttgtttct ggaaccttcg gtgaagttga tggtgtgcaa
    32221 atcgtgcgct ctaaaaaagt tgatgaaggt aaaggcttcc ttgttaaagt gtcaccaagt
    32281 caaactcaga cagacgacgc taacaaatat ggagcttttg ttatcttgct taaacgtgat
    32341 gtggctatcg aaacagaccg cgatatcttg aagaagacta ccgtaatcac aggtgatgaa
    32401 cactacggcg tttaccttta cgaccctaca cgagttgtaa aattcggtgg cgcgtaagag
    32461 gtgacgatat gagtttattg ctacgacgtc attatattca agaagagcag gttaaccagt
    32521 attctgattt agagaataaa actctagaag agttgaagga tctagcgaaa gaagcaggtg
    32581 tagcaggcgc ttataagttg acaaaagccg aaattgtaga agttttggag gaactaaaaa
    32641 gtgaaattta aaatcaaaca agatttctat gattgggaat caaatgtgaa acgactggca
    32701 ggagaggaac ttgagattac tgaggagcgc tatgctgagc tggctgacaa tattgccagc
    32761 aacggtgtca ctatctcaga cgttcttgag aaaatcctcc ctgaacctga gttcttagaa
    32821 gaggattgat atgtctatag agttgctgaa gaaattaaca ggcgaagaag atactcagct
    32881 tctcatgttg ctccaaacga gggctacaaa tcttatcttg tcagagacta atcgcacatc
    32941 tttgacacct gctttaagtc ttttaatacc tgaggttgct atcgaactcc acaaccgctc
    33001 aggagcggaa ggagagcatt ctagaaccga gggtggtata gcagtagtct acggagaaaa
    33061 cggcctgtct acggatcttc tacagcgaat acgcatgcac aggctagcaa gggtggcagg
    33121 tcatgttttt gaagcagagt agactgaaac cttatccaat gcgacggttt gaaaagactg
    33181 tcactgagga aggtgtcgcg aaagaagggt atgccaagga agctgagaca gtccgccttg
    33241 aattgtggcc agctagtagc aagctacaat ctgaattgta tggcgagcgt gtcaatgata
    33301 ttttgaacgc aaatgccaac aagtcagcta ctatcaaagt aaaagatggt gtgtgtatcg
    33361 atagtcagac ggaagtgact catagggtta tttctaaaaa ggtctacaca catcatcaag
    33421 ttttggagtt agagcgtgtc agggctacta ggggcagata ggcttatagc taaatgtaga
    33481 cgattggcta gtaaaaaaac tggcgaggat atcgtcttac gtgcggtgca caatgctact
    33541 ataaaggttg tccaagcaga tgcaagaaga ctcgcaccag cgagagatgg agagcttata
    33601 actagtatca aaactagggc aaaaatggac ggagataagg ctataggcga agtttacacc
    33661 aaccttaaat acgctcctta tgttgagttt ggaacgggac caataggaca agctagccat
    33721 tctggtattt ctccagaggt cagcgtgact tacaagtcta gtccgtggta tgtacatgaa
    33781 gaccaaatca atgtaggacc gtaccacttt caaaaaatcg gggagttcta caagatgtat
    33841 ggtcaacctg cccagcctta tctttatcca gctttgagag acaatcaaga gcgtgtgtct
    33901 aagaatattt cgaattatgt ccgtagaaag ataagagaac aaataaaatg attaatatca
    33961 agcctgttat ttataaagaa ttgcaaaagg ttgcagataa tgtgaccgat acttatccta
    34021 gcgattggga gactttccca gtcgttattt ttttggaaga acaaaacaag ccgggtgatt
    34081 ggtttgatga ccaggaacaa aaatcctcta tccgctacaa ggtggatatc tttgatgata
    34141 ctagcactag tgagttagct gttaaaatca atcagatttt tgagtcttta ggtttgcgaa
    34201 gaactgactg ccaagacgtg ccagacccgt ctcatttgag acataaggtc atgcgttttg
    34261 aaggtatcgt cgacttacac tcagagcttg tttttcaatt tagaatggag aattaaacat
    34321 gttagcaaat ggaattacgc tttcttatgg cgaaacaaaa gatagctata ctaaacttgt
    34381 tggattgaaa gaagtaccag agtttggtat tgaactcgaa aaagtagaaa atactactct
    34441 tgaagatacg gtgaagaagt acgagtttgg tattggggac ataggagaac ttgagtacaa
    34501 gttctcttat aataattcaa gcgcaactgc tccttatcgt gtattgcgta aggcagcaga
    34561 cgacaagaag aaactctact ttgaacaagc ttatccagac ggtactaagg tcatttttga
    34621 aggccaagta tctgttaagc ttggcggtgg cggtgtcaat gccgttatcg atttcacact
    34681 taagattgcc ttgcagtcta atcttacatt tactgatggt attggaggtt aattaaatgg
    34741 cgttaaaata cacaacttgg aaagttactg acgaaaaaga gttgaagcta cgtttgacat
    34801 ctcatcaagc tgcaactgtg gaagaaaaaa tcggcatgaa cttgctgaag attttcatgc
    34861 ctgaagctgg cgaagagttc actttaccgc ctttgaaagt tatgttgttg ttagttcacg
    34921 gagccttgca gcagtatgaa catgggtatt ctcttgagga tgtctatgat ttatacgatg
    34981 aatacgtgga caatggcgga gaccaaacaa ccttcatgac agaggtgttg atgccactct
    35041 ttgaagtatc gggttttact ccacgaggaa gcaaggacaa gaaaacttcc aagaagaaaa
    35101 tgacagtaga caagtaatct taacggtaac tcagattatt gagaggcttt atcctatgtt
    35161 tttggacatc gagggcaagc ctcttgattt ttgggatttg acggtacttg aaatcagaga
    35221 aatgattgaa agctataacc gtgtcaaaat ccaagagcgt aaagaaaaga ttattgactc
    35281 atacagactt tcgcagatga tatccaacca cgtttcttta ttgttatcca aagatgccaa
    35341 ggcctttgag ttctgggaat atgcgcctga gttgtttgta gaagaacaac aagcagtaga
    35401 acaggaacga cagaaacaag cacttttgtt gcataaggaa cggatgcgtg aatttgcaga
    35461 gagacataat cgaaaaagga aggaggaagt aaatggcaac tcttgatgaa ttgaaagtca
    35521 tgattgacgc tgagatagcg cctttcagga agaagatgaa agaagtcgag aatcaggtca
    35581 aaggaacatc tgaccaagtg aaaaatgcta ctgccaaagt tcgtgaacag tcgagctcaa
    35641 tcggtagtgc gtttggcaag ctggctaagt tcgctggttt tgcaatcctt ggtaagaaat
    35701 tacttgatgt tgggatgtat tcaacgcaga cggctcttga agtatcagcg tctatgaacc
    35761 aaatcaagcg acagatgggc gagagttcgc aatctttctt aaaatgggtt aacgataatg
    35821 ccaacgctat gaatatgggt gtgggtgagg ctaccaacta cggtgcagtc tactcaaact
    35881 tattttctgg atttatcaaa gataccaaca agctaagcgc ctataccgct aagatgttgc
    35941 agacatcggc agttgttgct gaaggttcag ggcgcacgat tacagacgtt atggagcgga
    36001 ttcgctcagg tttactaggg aacaccgaag cgattgagga cctaggaatc aacgtcaacg
    36061 tggctatgat tgagtccact gaagccttta agaagttcgc aaacggacag agctggcaac
    36121 agttggatta ccaaacccag caacaaatcc gccttatggc tattctggaa caggctacag
    36181 ccaagtatgg ggataccttg tctaattctg taaatggtcg tatcagccta tttaagtcgc
    36241 tgatgaagga cgcagcattg aaccttggta actctatgtt accgattatc aatgccatta
    36301 tgcctgtctt gaactctttt gctatggtct taaagaacgt tactgctaaa ctcgctgagt
    36361 ttatcgcttt gatgttcaac aagaaagcaa cagtgaaaga tggtgttggt ggagcagttg
    36421 gagacatggg taacgccatg aaggatgctg caggcggagc aggagacctt gctgacgcag
    36481 tagacgacgc tggagattca gcaggaggac ttgctgacaa tcttggagac tccgccaaaa
    36541 acgctaagaa ggccgctaaa gagttgctag gtcttttggg atttgatgag attaacatct
    36601 tgcaaaaacc aaaagatgac gacgcaggcg ggtctggagg cggtggcaaa ggtggtaaag
    36661 gaaagggagg cggtggcgga cctttcaaag acatcttgcc agaagtcgag ttgaccgaca
    36721 tggacaacaa attcaagagc atttttgatg gtcttggaga taagctcaaa gggttgtttg
    36781 acctcttcaa gaaaggtttt gatgcagcat ttagaccaga aggtatagaa cgcattaaga
    36841 ctgccttaga ccaaatagct aagacaatgg gagaaatcgc cactgaccca agggttgtga
    36901 atgcctttaa ccgaatggct gagaaaattg cttatgcttt agggcaagtg acaggctcaa
    36961 taacaactat cgggctaggt atcggtgttt tccttgccga aagtattgca aatggccttg
    37021 gaaggcaaaa agaacgcatt accagggcgc tagtcgcttt gtttgataat attggtaaca
    37081 tttccgaggc agtaggaaac atcgctcagg acttttctag tactttctac gacgtcatta
    37141 cctcaactgg tgcggttcgt atcggtagcg ctattgtgtc aactctgttg agtttgacat
    37201 ctaccattgt tgaagttggt agtaaattag caggaagttt gtttaaaggt tttgaaaaag
    37261 tcgttgtgac aagcgctcct aaaatttcat cagtcttcca aagtttatta gatactgttg
    37321 cgcctgtatt tgagagcatt gaaaggtctg ttaacaaatt tggcgatggc ttaagtcgtg
    37381 tttatgatga acatgtagcc cctgctatta actctattgc taatgctttt aacggactga
    37441 ttgatatcat acaaatcctc tgggagaatt cttggcaacc ttttgctgag tttttatcag
    37501 gagtattcgg tgttagtatt gaaggaattt cagatttatt aggaggtggc cttttagcca
    37561 ctttgggact attggcggat gctattaagt tagtggcaga tggtttcacc gttttttctg
    37621 actggtgtaa agaaaacaaa gaacctatct tggctttgat aacaacttgg caaacgatta
    37681 atttcttatc atgggctgaa caagccgggg gacttgcagg agcattcagc ttgttaggta
    37741 gtaaggtctc tttgattgtt ggagggatta agaatctagg tcttgctatt aaagcattga
    37801 catttgataa gttggtcagt tttggtgaaa caatctattt gaacacctta tatgcaaaag
    37861 attttgtggt caattcaggt aaaacaattg cacagctagg aaaaactgct ttagaacttg
    37921 gtaaatcagc tctagcatgg actgctcatg cagcgaaaat gggattagca accgcggcgg
    37981 aatttgcaca ttctgttgca gcaggagtcg ctacagctgc aacatgggct tttaatgcag
    38041 cgttagcagt tttgacaagt ccaataacat ggattattgc agcaatcgca gctttgattg
    38101 ctatcggtgt cttgctctac caaaactggg acactgttgt tgagtttgct aaaactgcat
    38161 ggcaaggact atgtgatttt attagtggta tttgtcgagc tattggcgaa tttttcagtg
    38221 gtctatggac aaaactacaa gaaatctttg agccgatagg tcaatggttt ggcgagaagt
    38281 tccagcaagc atgggatgcc attgtcaaca tcttctctgg tatcggagag tggttctctg
    38341 gtgtattcca aggtgcatgg gacgctatcg ttaatatctt cactccaatc ggctcatggt
    38401 ttggacaacg ttgggcagat gtgactagtg ctttggctaa tatcggggca tggtttactg
    38461 acatattcca aaaagcatgg actggtctaa caaacatctt tagcaaacta ggtttatggt
    38521 ttggcgagag atgggcagat gttacaagtg ttcttgcgaa tgtatcttct tggtttggga
    38581 atatgtttac tagtgcttat aatgcagtca agaacgcgtt tagttcaatt ggtggcttct
    38641 tcagcggtgt atggtcaacg gttcaaagca tatttgtcaa tgctggacaa aaggttggta
    38701 gcgctgtagg tggagcgttt aagagtgcgg tcaatgcggt tcttggaacg attgaaaatg
    38761 tagtcaatga cttcatcgga atgattaatg gagttttagg cgttgtcaga aacttacctg
    38821 gtctaggatg ggttggtagt gtaagcacag ttagcctccc tcgtcttgcc cgtggtggta
    38881 tcgtcgatag tccaacaatc gccatgattg gtgaagctgg taaagaggcg gtcgtaccac
    38941 ttgaaaatac aggatttatc caaacacttg gacgagtagt cagcagtgcg gtagtaaatg
    39001 ccatggctgg tattagtcca caaggtggtt tttctagcga cggcgacatc gttattcaaa
    39061 tcgcaggcca tgagttcgga cgggtagcta tccaagaaat caacaaggaa catgaacgag
    39121 caggtcaaac cttgctcaag atttaggagg ttaaatggca caattgacaa tcaatggggt
    39181 ggctgtgaag cctcccaaat cttttcaagt cggtattcaa gatatcgatg gagagacagg
    39241 gcgtaatgcc aatggcgaca tggtgcgtga ccgtatcacg accaaacgca aactagactg
    39301 tgaatggggt atgatgactc agggagaaat aagtcagctt ttacatgctg tatcatctaa
    39361 attttttgag gtatcttatc cagaccccat ggatggccaa gtcacaaaga ctttctatgt
    39421 cggtgatagg acagctccta gctatacctt tactgagaag cttaaacctt ggtctggcgc
    39481 taaatttaat ctggtagaga ggtaagaaaa tggacgcttt aactagacga caatttgaca
    39541 gagccatgtt tgccaaggaa aggacgctgg ctattcgtgt tggtgaatat gcttcacggg
    39601 atatcaaaga ggctagtttt gagtatggct acatcaaggg cgatacttat aagcctggtg
    39661 gaacctgcgc tggtagcggt aaaattacct ttaccagtat cattaccacg ttcaataagc
    39721 tggataccct gcaccctgag attggtctac tggttgggga tacctaccag tgggtcaaga
    39781 tgggggaata cttcatcaac gatattgaga ttgaccgaaa ccgcaacaca accacacttg
    39841 aacttatgga cggtatgttt aagctcaatc gtgagtacgt gacggatttg catttcccag
    39901 ctgaagtacg agaggttatt caggaaatct gcctgaaaac aggcattgag ttagcgaatg
    39961 actatttcgg aatcagcgct atgcgttacc atatcgagca agttcctgaa ggtaagaaac
    40021 tttcgttcag ggatatgctg agcgctatga ctcagatgat tgggatgtct tgcttcttca
    40081 accgagaagg aaagatggaa atccgtgatt tgaccgagtc caacatcacg attaacgctg
    40141 acagttactt cttgcatgga ttgaccaaga gtgaaatcga gtatcagata gctggtatca
    40201 cttgtaagac ggataagaag tctctgacgg tcggtatgac aacaggtcgg tctttggaac
    40261 ttgataatgt cttcataacc cagagcgctt taaatgactt gtattacaaa ctgaaaaacc
    40321 taacttacta tccgtataat ctcaactacc aaggacattt gttacttgag gttgggcagt
    40381 gggtaaccat tcagaccaac aagaaagaga cttttaaagt tcctgtctta agtcagagct
    40441 ttatctttaa aggtggtctg agaggtcgta tcagtgcaga tagtaaggct ggaaacgata
    40501 cccagtattc ttacgagggt acgattacca agcagattaa gcaacaagat ggctttgaag
    40561 cgaaaatcca agcgcagatt gaagcagcag ataaagattt tgaccaaaag gtcgacaaaa
    40621 tcaaaaaaga ctttaacgat caagtagaac tggccaaagc cagagctgaa gaagtcaaga
    40681 gagaactgtc tgacactatc aatcagcgct ttaatagctt tgacaacggg ccattgaaag
    40741 aagctaagcg caaggctgag gaagctttgc gaaatgctgg cgcaagtagt tctcttgctc
    40801 aggaatccaa gcggattggg ctggattctg ttgctagact tgaagcgttt aagtcgcaga
    40861 ctacgagcgc acaaacggct ctgtcgggtg acttggatgt tctaaaacga actatcgcaa
    40921 acgatattcg accgaagcaa gcacaggctg aagctgagat tgccaagcaa gttgaagcac
    40981 ttagccggac taaaaatgaa ctggatggcg caagtaccct gcttgcacag gaagctaagc
    41041 ggattgagct ggattctgtt gctagacttg aagcgtttaa gtcgcagact acgagcgcac
    41101 aaacggctct gtcaggtgac ttggatgttc taaaacgaac tatcgcaaac gatattcgac
    41161 cgaagcaagc acaggctgaa gctgagattg ccaagcaagt tgaagcactt agccggacta
    41221 aaaatgaact ggctggcgtg aagtcagcgc aagcgacgta tgaggagacg acgactcgta
    41281 gactgtcaga actgaccaac ttggccaatg gtaaagccag caagtcagaa ctcacgcaga
    41341 cagctgagga gctagctagt cggattgcga gtgtgcaggc atccggtcga aatctattct
    41401 tgaactcact attcaagcag gatatttcaa aaacaggaat ttggacaacg agtacatata
    41461 cggctactat cgatagcgaa agtaagtatc ttggatataa cgctcttaaa attataggtc
    41521 tgaatccatc tggccgtgat ggagggaatc ccaaggttac ttatccagtt ctgggtcaat
    41581 tcgggaaagt aattcccgga agtacgacta atcaagatgt aaccattagt ttttatgcta
    41641 aggcaaataa aaatggaata atgctaagat ctcgattagg gaatatcgga tataaaactg
    41701 gaaatgtgac attgtcgaca gaaattaaac gatatgttgt ccatattcca aaaggttgga
    41761 caaacgaatc caagcagacc acaaatgaat ggttgttcaa tttcaaccag gaaggaaccg
    41821 tttggatttg gatgccgaag tttgaaataa gcgatgtaga tacttcttat tcagaagctc
    41881 ctgaagatat agaaggtcag atttcaacag ttgaatcgac ctttaaacaa cgagtcaact
    41941 cgctcgaagc tggtgtgaat cgtctgactg aaggccttag aaccaaagcc gatatcagcg
    42001 cactcaacgt gactgctgaa aatatccggc aatctgtgaa gagtcttgag acagacacgc
    42061 agaacaagct aaatcagaag ttgagtcagg ctgaatttga ggtgcgagcc ggctctatcc
    42121 gtcaggaaat cctgaacgca accaaggata aggcagataa gactttagtt gtatctgaag
    42181 ctgggaaatt gcgtgaagaa ttttcaaaaa tgaaggtggg aggccggaat ctatggataa
    42241 aatccaagac ggttggagct gtaattgaaa aattacctga aaaccatgtc acaggtcaaa
    42301 aagaatgtta taggttagag aacaactcta ctttaacgtt caaccttgaa ccagatttca
    42361 gctcaaggtt gtatcaaaaa gttactttta gcgcttggat caagtacgaa aatgtagtcc
    42421 aaggtcgaaa cttttggaat gtatttaatt gcttcaaaca ttatcttttt agaaaaaata
    42481 gtgagaccgg agtacagagt ggtccagatt atgctacgct tggtatgtat aaaggttcgg
    42541 cagattggaa atatattaca ttcacttatg actactctga aaaaacaaat tttgatcaat
    42601 tgaagacatc attgcgattc aatcttgaag gtgctacaag cggtacagct tgggtaacag
    42661 gaatcaaggt tgaaatcggt agtgtggcga cggactggtc accagcacca gaagacggag
    42721 aaaatgagct tttagtcgct aaaaccgagt tcaagaggac agctgatggc ctatctacta
    42781 agatggcagc agttgagagc tatgttggtc aagacggtca gcgacaggaa gccctacagc
    42841 gctatactcg tgaggaaagc acgagacaag cgacagcagt ccgtgagctg gtcaatcgtg
    42901 atttcgttgg taaggctact tatcaagaag atgttaaggg tatcaatcag aggattgaag
    42961 ctgttaaaac tagtgcgaat aaagacatcg ctagtcaaat cgctagctat cgtcaatctg
    43021 tagatggtaa gttcacggat atttcaagtc agataactac ttataagcaa gatgtgggcg
    43081 gtcaaatcag tggtctatca aatagactta caagcagtga gcaaggaacc actactcaga
    43141 tttcaaatat ttcaaatcgg ataaacagta ataagcaagg cacagataat cagatttcaa
    43201 atttaaagac tcaggtcgct acaaacaagg ataatgctga acgacaaatg ggtagaatat
    43261 ctgatcaggt ttctgcaaac aaagcgaatg ctgatagtca atttgcgaat gtgaccaatc
    43321 aactagcgcg aaaagtagag actactgact tccagcgtgt taaggaaacc agtaaacttt
    43381 acgagcgaat tttgggcaat actgaaaatg gaattgcgga taaggttgct cgcatggctc
    43441 tgactaatca actgtttcag gttgaggttg ggaaatatag tgtaagcggc cctaacctca
    43501 ttaagaatag tgattttaaa aatgctacga atgaatgggg ctcaactcaa aatttaggaa
    43561 gattggttaa gcatagcttt tatcacaacg ggcagaaaga ccttatgcgt ttaagtaatg
    43621 caactaaaaa cgaaaacttt ttgtatagtc accgttttaa tcttgaacga aatactgact
    43681 atgtactgaa ttttagagga tttaacaaca gtgctctcgc aagctatgat gtttatattt
    43741 tgggacgaag agcaggcgag agcgatggat tcacaatcgt taagaaagtt gttagcagca
    43801 agaaactatc tacctctaga tgcgaagatg tctcagtaac ttttaattcc ggagaaatgg
    43861 ataatgctta cattcgtttt gataacaatg gctcatcatc aggaacagct gatttgtata
    43921 ttacagaagt tgacttgtac aaaggttata aacctagaac atggcaacca catccagaag
    43981 atgcagtcgc agatgcgaat aagaagcttg aagcaacgca aacaaaaatg actcaactag
    44041 ctggctcatg ggtagttgaa aacatcaact cggctggaga tatcatctct ggaatcaatc
    44101 ttggcgccaa tggacataac cgcttcgttg ggaaattgac ccacatcact ggagagaccc
    44161 tgattgacag agcagtcatc aagtctgcca tggttgataa gctgaaaacg ggcaattttg
    44221 aagctggttc ggtcacgact acgatattag acgctgaagc ggtaactgct gagaagttga
    44281 aagttgacga tgcgcttatt agaaaattaa ctgcaaatga tgcttttatt gaccgactga
    44341 catctaaacg tatcttctct actaaggttg agtccgtcat ttctagttca accttcctag
    44401 aagcctatca aggtcgaatt ggtggattca cacttggtca atttgaccag ggtggcggtc
    44461 gctggatttc aggtgtcaat cagttctctg ttggtatggg gaatggtgcc gggcatggag
    44521 tccggacagc cttctgggcg aactggggaa ataattggaa ctatgccgga cctaaagcat
    44581 ggaacgtcaa tactgatggg aaaatgtact gtaggaatga agtcggtttt tatgatcaag
    44641 tggatttttc gaattcatcg agagcaaact tctatgggaa tactactttt tctcgttctc
    44701 ctgtgttttc aaatggtatc gaacttggaa gtaaagatgt gcttggtgat ggttggaatc
    44761 ccaaaggcgg aaggaatgcg gttgtttggt ggaatcaggt cggtagcggt agcgtgaagt
    44821 attggatgga acaaaaatca gacagacgct taaaagagaa catcacagat acagctgtga
    44881 aagccttgga caaaatcaac agattaagaa tggttgcatt tgatttcatc gaaaataaga
    44941 aacatgagga gattggtcta atagctcaag aggctgaaac catcgttcca agaattgtct
    45001 cacgagatcc tgagaatcca gatggctatc tgcatattga ctataccgct ttagttcctt
    45061 acttaatcaa ggctattcaa gaattaaatc aaaaaataga aaaaatggag aaaacaatag
    45121 catgaataac aacatgttga ccaatatcgc actttaaagc aattcaggag cttgctcttg
    45181 aaaatagaaa acgaacacac agattggaga acttagaaaa tgaacacaga acagcttaac
    45241 caagccttac aaatgacaat tagtgaaatg tcaacaactt caacaaattc gatgattaca
    45301 aataatatct tgagtattca gttggatgag caaagggaag ggaatcaaag acttcaagca
    45361 cgagtggatg agctggaagc tctgcttgat gaacaaacta aaccagcaga caaaggagaa
    45421 tagacatggc agaaacaaat caaaacacag ataacttact agaccttaca aaaatcacag
    45481 aaccatttga tcttgcgagt gctttgcgct acatgaaaga aaatggagag ttcattcgtt
    45541 gcaagaatgt aagcgatgac ttctatatgt atcgtgatgt tcaaaaacgt cctgtgatcg
    45601 taaatggccg tcgccaactc aaggatgttg aaaccgtttg ggcgttcaac cagtggggtg
    45661 gtacaatcgc aacaatcaac gtcgccgttc tgttgaatca tgaattctat atcatgaaat
    45721 ttgatgcaga gggcaatcct gactggacgg atccaacggt aaaacctaaa gaataggagg
    45781 tgtgtatgcc aattgaagaa gctgaaaaaa tcgctcaaag tcaggtagct tgggcgattt
    45841 tgtttatctt gcttttcttt attatcattc gatatcttat caagacttcg gacaagcgag
    45901 agaagaagat tatggatttg cacgagcaat caaaggccga ctctaataga cgagaagagc
    45961 gtttgatgac tcacctagaa aagaccacta cagaattaac cacaatcact cacgcggtcg
    46021 gagacattca aaaagaaatg gttcgcatga acgaccgcat ggaagaaatc gaaaaaggag
    46081 aataacaaat gcaacaaatt actgaaatca ttactaatgg agcaatcagc atccttgtta
    46141 ttttggctgg tatcgcagtc aaggcagtca aggactacct ggttcaaaaa ggtggagaaa
    46201 agaccatcaa aatcgttgaa atcttggcca aaaatgcagt aaatgccgtt gagcaagtag
    46261 ctgctgaaac tggctacaag ggagatgaaa aactggcaca ggctcgcgct aaagtccgtg
    46321 ctgagcttac aaaatacaat attagcatga ctgacaaaga cttagacacc ttcgtagagt
    46381 cagcagtgaa gcagatgaat gacgcatgga aaggacgata gggaatggat atcgatagaa
    46441 acagactacg tacaggcttg ccacaggttg gggtgcagcc ttatcgacaa gtacacgccc
    46501 attcaacagg caaccgtaac tcaaccgtac agaatgaagc ggattatcac tggcggaaag
    46561 acccagaatt aggttttttc tcgcacgttg ttgggaacgg tcgcatcatg caggtaggac
    46621 ctgtgaacaa cggaagttgg gatgttgggg gcggttggaa tgctgagagt tacgcagcgg
    46681 ttgaactgat tgaaagccat tcaactaaag aagagttcat gacggactac cgcctctata
    46741 tcgaattgct acgcaatcta gcggacgaag caggcttgcc gaagactctt gatacagacg
    46801 acttggcagg tatcaagacg catgaatact gtaccaataa ccaaccaaac aaccactcag
    46861 accatgtgga tccatatcca tatcttgcta aatggggcat tagccgtgaa cagtttaagc
    46921 aagacatcga aaacggcttg agagctgcaa caggctggca gaaaaatggc actggctact
    46981 ggtacgtaca ctcagacggc tcttatccaa aagataagtt tgagaaaatc aacggtacct
    47041 ggtattattt cgatggctca ggctatatgc tttcagaccg ctggaagaag cacacagacg
    47101 gtaattggta ctactttgac caatcaggcg aaatggccac aggctggaag aaaatcgctg
    47161 agaagtggta ctattttgat gtagaaggtg ccatgaagac aggctgggtc aagtacaaga
    47221 acacttggta ctacttagac gctaaagaag gtgctatggt atcaaacgcc ttcgtccagt
    47281 ccgcagacgg aacaggctgg tactacctca aaccagacgg aacactggca gataagccag
    47341 acttcacagt agagccagat ggcttgatta cagttaaata aatagaaagg aaactttcta
    47401 aattgttctt tcaccgcagg ctcaggcttg cggttttttt gttttaaaaa aggggcaaaa
    47461 aaggggcaaa agtgtcgtaa atctctgtaa aatgatgtaa aaacatttat ttaaaagctc
    47521 aaaatatagc tgttttaaaa ggtattgtaa gatatagtaa aacgatgtaa aggtattttt
    47581 aaatgcggat gaattataaa accacgaatc ctatgtgact cgtggttctt ttttataaac
    47641 tggtagagtg ttttggttgt actttttgtt cagggtcaat gtagttgata gcgttgttga
    47701 cagcagttgg agcttcccct agacctgtcg caatcagatc aatttttccg tcatagtagc
    47761 agcagtcacc gatagcatag atacctgctt ggctggattc ttgtttgctg ttgacgataa
    47821 tcttgtgacg gttgaggtcg agcccccagt ttttaaggtt accgacagaa gatttgaaac
    47881 catagttgac aaagaggtgg tctaggtcaa tggtttcagt ttcatcagat ttgacttttg
    47941 tgatttcaag tttatcaagc gtttttccat ctccaaggag ttggctaggg acgaatggtg
    48001 tcttgatggt tacagatgat tcttgcaagg cttgaacact gtgttccaag gcacggaaat
    48061 tatctctgcg gtgaacaagg gtagttggtg cgattttttc aaaagccaaa gtccaatcca
    48121 cagccgagtc tcccccgcca agaatcgtca ctttcttacc agcgtattgc tgaatgttag
    48181 aaacgtggta gtggatattt tcatagccct caacactttc aagttccagc ggacgtggtt
    48241 tgaaggcacc gccacccata gcgatgataa ctgttttagt caggtgactt cctttagaag
    48301 ttgtgatggc aaattcttct tcttgtttgt caatctcaag aaccgtttca ttgagatgaa
    48361 taggggtatc aaatccattt agctgttcaa tcaagcggtt agtcaactct tctccagtca
    48421 ggtttgggaa gcctggtacg tctaggattt ccttttcagg gtagagaata gcaggttgtc
    48481 cacctagctg gggaagagag tcgatgattt gaaccttggc ttggcgtagg tgggcataaa
    48541 aggctgcaaa aagcccgaca ggaccaccac ccacaatggt aatatcatag agttgagaca
    48601 tagtttctcc tttgtttttt ctagtcagtt tattttatca tatttttgtt caatgtaaaa
    48661 tggactagga tagggaaaaa aatgccagaa aatggtataa taggacgaaa gtgttttgat
    48721 agggaggata tcagggatga attttcaaca attatccaat ctgcaatatt ggaccagttt
    48781 gtttgcaagt ccatggacga tagctatcaa tctgattgat attttgattg ttgcttatat
    48841 tttataccat tttacaaaag ctattgcagg aaccaagatt atgattttgg tacgtggagt
    48901 tttggtgttt attttagctc aaatccttgc aaatatgatt ggtttgacta cgatttcttg
    48961 gttaatcaat caaattatta cttatggggt tattgcggcg gttgttatct tctctccaga
    49021 gattcggact ggtttggaac gtttgggaag agcgacagat tttttttcca atgcccctat
    49081 tagtgctgag gaacagatga ttcgtgcctt tgttaagtct gttgaataca tgagtcctcg
    49141 taaaatcggg gccttggttg ctattcagcg tgtacgtacc ttgcaggagt atatttcgac
    49201 aggaattccc ttggatgcta agatttctgc agaacttctc attaacattt ttattcccaa
    49261 cactccccta catgatggtg cggtgattat caaagaagaa cgtatcgctg tgacgtctgc
    49321 ctatctgccc ttgacaaaaa acacagggat ttccaaggaa tttgggacca gacaccgggc
    49381 ggctatcggt ttatcagaag tctcagatgc cttgactttt gtcgtatcag aggaaacggg
    49441 aggaatttcg ataacctata atggaaggtt taagcacaac ctaacacttg atgaatttga
    49501 aacagaatta cgtgaaatct tacttccaaa agaggaagtg ggtcttagtt ttaaagaacg
    49561 attgctagga ggatggaaac atgaaaaaaa atagtttata tatcatatcc tcactctttt
    49621 ttgcttgtgt cttatttgtc tatgctacgg cgacgaattt tcaaaacagt accagtgcta
    49681 ggcaggtaaa aacggaaacc tatactaata cagtaacaaa tgtccctatt gacatacgct
    49741 ataatagtga taagtatttt attagcggtt ttgcttcaga agtatcagtg gtcttgactg
    49801 gtgcaaatcg cctatcgcta gctagtgaaa tgcaagaaag tacacgtaaa ttcaaggtta
    49861 ctgctgacct aacagatgcc ggtgttggaa cgattgaagt tcctttgagc attgaagatt
    49921 tacccaatgg gctgaccgct gtggcgactc cgcaaaaaat tacagtcaag attggtaaga
    49981 aggctcagaa ggataaggta aagattgtac cagagattga ccctagtcaa attgatagtc
    50041 gggtacaaat tgaaaatgtc atggtgtcag ataaagaagt gtctattacg agtgaccaag
    50101 agacattgga tagaattgat aagattatcg ctgtcttgcc aactagcgaa cgtataacag
    50161 gtaattacag tggttcagta cctttgcagg caatcgaccg caatggtgtt gtcttaccgg
    50221 cagttatcac tccgtttgat acaataatga aggtgactac aaaaccagta gcaccaagtt
    50281 caagcacatc aaattcaagt acaagcagtt catcggagac atcttcgtca acgaaagcaa
    50341 ctagttcaaa aacgaattaa aaatagaaaa ggattttata acaatgggta aatattttgg
    50401 gactgatgga gtccgtggag aagctaacct agaactaaca ccagaattag cctttaaact
    50461 aggacgtttt ggaggctatg ttcttagtca acatgaaacg gaagcgccga aagtctttgt
    50521 aggacgtgac acacgtattt caggggaaat gctggaatcg gccttggtgg caggtctcct
    50581 ttcagtaggg attcacgtat acaaacttgg tgtccttgca acaccagcag tagcttactt
    50641 ggttgaaact gaaggagcaa gtgccggtgt catgatttct gctagccaca acccagccct
    50701 tgataacgga atcaagttct ttggcggtga tggcttcaaa ctagatgatg aaaaagaagc
    50761 agaaattgaa gccttgctag atgctgagga agacactctt cctcgtccaa gtgcagaagg
    50821 cttaggaatc ttggtagatt atccagaagg cttgcgtaag tatgaaggat atcttgtttc
    50881 aactggaact cctcttgatg gaatgaaggt tgccttggat acagctaatg gagcagcttc
    50941 taccagtgcc cgtcaaatct ttgcagacct tggtgcccaa ttgacggtta tcggggaaac
    51001 accagacggt cttaacatca accttaatgt tggttcaaca catccagaag cccttcaaga
    51061 agtggtcaaa gaaagtgggt cagctattgg tttggccttt gatggagaca gtgaccgctt
    51121 gattgctgtt gatgagaatg gtgacatcgt cgatggtgac aagattatgt acatcatcgg
    51181 aaaatacctt tctgaaaaag gacaattggc tcaaaataca attgtgacaa ctgttatgtc
    51241 taaccttggt ttccacaagg ccttgaatcg cgaaggtatt aacaaggcag ttactgcagt
    51301 tggtgaccgc tacgttgttg aagaaatgag aaaatcaggc tacaaccttg gtggtgaaca
    51361 gtctggtcac gttatcttga tggattacaa taccacaggt gatggtcaat tatcagcagt
    51421 tcaattgact aaaatcatga aggaaactgg taagagctta tcagagttgg cggcagaagt
    51481 aacgatttat ccacaaaaat tagttaatat ccgagtggaa aacgtcatga aggaaaaggc
    51541 catggaagtg ccagctatca aggccatcat cgagaagatg gaagaagaaa tggcggggaa
    51601 cggccgtatc cttgttcgtc caagtggaac agagcccctc ttgcgtgtta tggcagaagc
    51661 gcctacaaca gaagaagtag actactatgt tgataccatc acagatgtag ttcgtgctga
    51721 aattgggatt gactaaatcc tagaaaacta gatgaaaata aaaaattatg atacaatagc
    51781 ttataatatt gtagccgatg gagaaagaca tgaatcttaa acgtgaacaa gaatttgtta
    51841 gtcagtatca ttttgatgcg cgtaattttg aatgggaaaa tgaaaatgga gctcctgaaa
    51901 ccaaggtgga tgtgaatttc caattgctcc aacatgacca agaaaatcaa gtgacttcct
    51961 tgattgtcat cttgagcttt atgattgtat ttgataagtt tgtcattagt ggaacaatct
    52021 cacaagtaaa ccatatcgat ggccgtattg tcaatgaacc aagtgaattg aaccaagaag
    52081 aggtggaaac cttggctcgt ccatgtttga acatgctcaa tcgtttgact tacgaagtga
    52141 ctgaaattgc attagactta ccaggaatta atttggagtt ctaatatgaa attagctgtt
    52201 ttcacagatt cctctgctta tctcagtgca gagaccttgc aaagagaaga cttgtttgtc
    52261 ttggatattc ctgtcaatat tgatggtgag gaatatgtcg aaggcatcaa tctgtctgct
    52321 gaggaatttt accaaaaaat ggctcaggct tctgaatttc ctaagaccag tcaaccaagt
    52381 attgccaagt tagatgaaat cttaacttcg ctcaaagaac aaggctatac acatgccttg
    52441 gggcttttcc tatcttctgg aatttcaggt ttttaccaaa atatccagta tatggtcgat
    52501 gactatgagg gcttaaccat tgctttccca gacactttga ttacaagtgc tcccctaggc
    52561 atcatggttg aaagcgtctt taattggcgt gatcagggcg atgattttgc cagcattcag
    52621 gataagctag ccattcaaat cagccgtacg tcagccttta tcatggtaga tgacttggat
    52681 catttggtaa aaggtggacg cctttcaaat ggggctgcca ttttgggcaa tttgcttagc
    52741 attaagccaa tcctttattt taacgatcaa ggtgtgattg aagtttacga aaaagttcgt
    52801 actgaaaaga aggccaccaa gcgcttaatt gaaattatca aggaaacaac ggcttcaggt
    52861 caataccggg tcattgtcat tcatgggaat gctcctgaaa aggctgaaga attgcgtcag
    52921 cacttgcttg attttggctt gggttcggat gtttcacttg ctacatttgg tagtgtcatt
    52981 ggaacgcacc taggagcagg aagtattgct ctgggttata ttccagtgat ttagttggcg
    53041 cttctaggct agttaagatg ccttgtatct tagaaattga acacggacta cctgcctctt
    53101 gaaaaaagat gcctgtcttg cctttcgtgg aaagtcagtg ccattcccta tttttcagag
    53161 gcagctaagg tcctttgtat cttgataatt gaacacgcct ggaaccctgt gtgaaaaaga
    53221 tagttcttcc aagaagtaga cacttcttga ttggaattcc tattttcact ttgtgttctt
    53281 acggactttg tatcttagac aggagtagag atgagtattc gagtaattat tgccggtttt
    53341 aagggaaaga tgggccaggc tgcttgtcag atggtattga ctgatccaga cttggacttg
    53401 gtggcagttt tggatccttt tgagtctgag tcagaatggc agggtattcc tgttttcaag
    53461 gataaggctg atttagctgg ttttgaagcg gatgtctggg tagattttac tactccagct
    53521 gttgcctacg aaaatacacg ttttgctctt gaaaatggct ttgctccagt agttggaacg
    53581 actggtttca cgagtgaaga aattgcagag ctaaaagaat tttctcgtgc ccaagacttg
    53641 ggtggcctga ttgcccctaa ctttgccttg ggtgctgtct tactcatgca atttgcgacg
    53701 caggctgcca aatatttccc aaatgtggag attattgagc tccatcatga caagaaaaag
    53761 gatgctccga gtggaacagc cattaaaaca gctgagttga tggcagaggt tcgagagtcc
    53821 attcagcaag gtgcagcaga tgaggaagag ctgattgctg gtgctcgtgg tgctgacttt
    53881 gatggtatgc gcatccactc agttcgtttg ccaggcttgg tagctcatca ggaagtcatc
    53941 tttggcaatc agggagaagg gttgaccctc cgtcatgact cctatgatcg catctccttc
    54001 atgacaggag tcaatttggg aattaaagaa gttgtcaagc gtcatgagct tgtctatgga
    54061 ttagaacact tattatgaga ttaatgcaaa tgccttctga atttcagaag gctttaccag
    54121 tattagaaaa aattaaagaa gcaggctttg aggcttattt tgttgggggc tctgttcgag
    54181 atgcccttct ccatagccct atccacgatg tggatattgc gacgtcttct tatccagaag
    54241 agaccaagca gatttttccg cgaacagccg atatcggaat cgagcatgga accgtcttgg
    54301 tcttagatgg ggatgaggag tatgaggtaa caacctttcg gacagaggat gtctatgtgg
    54361 actatcgcag acccagtgcg gtttcctttg tgcgctcgct agaagaagac ctcaaacgcc
    54421 gtgatttcac agtcaacgcc tttgccttgg atgagacagg agaaatcgtt gacttgttcc
    54481 atggtttaga agatttggaa aagcaagtct tgcgagcagt tggagtggct agtgagcgtt
    54541 tcaacgaaga tgctttacgg attatgcgtg gtttccgttt tcaggctagt cttggttttg
    54601 cacttgagcc agaaacattt aaagctatga agaccttgac gccgcttttg gagaaaattt
    54661 ctgtagagcg taccttcgtc gagtttgata aactcttgct ggctccattt tggagaaggg
    54721 gcttggcttc catgattgag agtcaagctt atgactatct ccctgatatg gcatctagcc
    54781 aggacaagct caacagactg tttgatttgg agactgattt tacttttgaa tcctctgaac
    54841 aagcctgggc ggctttacta tgggctttgg agattgaaaa tgcgcagtca tttttgaaat
    54901 cttggaagac ctcacgccag tttgccaagc aagttcagga tttgctgatt attttggctc
    54961 tgcgtgaaaa tggagaattg agtaagcgtg attgttatcg ctttgacttg gatttacttt
    55021 tacaggctga aaatcttcgt caagctcaag gaaaagaagt caacccacaa gtcattaaag
    55081 aaacctacca gagcttgact attcatgata agaaagaaat ccaaatcaac ggcggtattt
    55141 tgatcaagga atatggctat cagccaggcc cagacttggg agagatttta acagagattg
    55201 agtttgccat tgtcgatgga gaattggaga ataatcgtga agccatccat gcttacctga
    55261 gggagaaaaa atgagtgatt ttatcgttga aaaactaagt aaatccgttg gtgacaagac
    55321 cgtttttagg gatatttcct ttattatcca tgacttagac agaattggtt taatcggtgt
    55381 caatgggact ggcaagacca cccttttgga cgtcctttct ggtgtttctg gatttgatgg
    55441 ggatgtcagt cctttttcag ctaaaaatga ttaccagatt ggttacttga ctcaggatcc
    55501 tgattttgat gatagaaaga cagttttgga tacggttcta tctagtgaac tcaaggaaat
    55561 ccagctcatt cgtgagtatg aattgattat gctcgactat agtgaggaca agcaggcgcg
    55621 tttggaacgt gtcatggcag agatggactc tctccaagct tgggaaatcg agagtcaggt
    55681 caagaccgtt cttagcaaat tgggcattca agacttatct actcctgttg gggaattgtc
    55741 aggtggtctg agaagacggg tacagttggc acaagtctta cttggcaacc acgacctttt
    55801 gcttttggat gagccgacca accatctgga tattgcgatt attgagtggc tgaccctctt
    55861 tttgaaaaat tctaagaaga ccgtcctttt tatcactcac gatcgttatt tcttagacgc
    55921 tttgtcaaca cggattttcg agttggagcg agcaggcttg accgagtacc agggaaatta
    55981 ccaggactat gttcgcctaa aggcggaaca ggatgagcgc gacgcggctc ttcttcacaa
    56041 aaaagaacaa ctctacaaac aagaattggc ctggatgcgc agacaaccgc aggcgcgtgc
    56101 gaccaagcag caggcccgta tcaatcgatt ccatgatctg aaaaaggaag tttcaggcag
    56161 tagtgctgag acagacttga ccatgaactt tgaaaccagt cggattggga agaaagtcat
    56221 cgagtttcag gctgtttcct ttgcctatga aaacaagcct attttgcaag attttaatct
    56281 cttggtgcag gccaaagacc gtatcggaat cgttggggac aatggtgttg gaaaatcaac
    56341 cctgctcaac ttgattgcag gaagtcttga gccaactaaa ggacaagttg tgattgggga
    56401 aactgttcgc atcgcctatt tctctcagca aattgagggt ttggatgaaa gcaagagagt
    56461 tatcaattac ctgcaggaag tggcagaaga ggtcaagacc agtggcggtt ctacgacttc
    56521 gattgcagag ttgctggagc agttcctttt cccacgttcg acgcatggaa ccttgattga
    56581 gaaattgtca gggggtgaga aaaaacgtct ttatctcctc aaactgcttt tggaaaaacc
    56641 aaatgttctt cttttagacg agccaaccaa tgacctagat attgcaactt tgacagtctt
    56701 agagaatttc ttgcaaggtt ttgcaggtcc cgttttaaca gtcagtcacg accgctattt
    56761 cttggataag gtagcgacca agattctcgc ttttgaggat ggcaagattc gtcctttctt
    56821 tggtcattac accgactatc ttgatgaaaa agcttttgaa acagatatgg ccaatcaagt
    56881 gcaaaaggtc gaaaaggaaa aagtggtcaa ggttcgagaa gacaagaaac gcatgaccta
    56941 ccaagaaaag caggagtggg caagtattga aggtgatatt gaaaccttgg aaaaacgtat
    57001 cgctgctatt gaagaggaaa tgcaggctaa cggctctgac tttggtaagc tgactactct
    57061 ccaaaaagaa ttggatgaga aaaatgaagc actccttgaa aaatacgaac gctatgagta
    57121 tctcagtgaa tttgatagtt aagactattc gaaaatcaaa gtgcaaacta tgtcggcttc
    57181 acattgccga ctcaagtaca gcctgtgacc ggcttcctaa tttgcacttt gatttttatt
    57241 gagtataaaa gaatagaaaa atcccgtctc atgaaaggat ttctctattc ttttttggtt
    57301 tcttgatgaa agatattttg ccaggtttcg tggcgacgcc agatactggt atggatgata
    57361 ccatctaact catagcagat gagtttggtt ttttgactga tggaagtaat ttgaatgtcc
    57421 tttatagcag aatttaactc ttttttggtc ttataagcct ctttatccat ctgctctcca
    57481 tcttggcgaa tatagacaaa atcatcagtc aagagttctt ctagttggtt tccttggtca
    57541 atcaattgtt cacgcataag caatttttta tagacattgt ctaaaatttc atcctcaggg
    57601 ataggtgctg gttcgatatg gacatcggta tcaaagacgc caaaacgctc ctccagcata
    57661 gactcgacct gatccgcgat ttcatggctt tcaaaaacag acaagtcagg attcatctct
    57721 agtgtaatat ccaggtagat gttgctaccg taggtgcgac ctctttgcga tttgaccttg
    57781 ctgattttgg gaatttccat gatagccttt tggtagtcct cgagcaggcg gtcgtcaaag
    57841 ccatctgaaa gactaaagga agactcgatg aagatatcat aggcagtctt caagataaag
    57901 aaagtgatga tgatagcaac cagtttatcc acaatcggat aattgaaact actagctagg
    57961 atggcaatgg cggttccaag tgaggtaaca gcgtcagaaa gattgtcctt agcagctgcc
    58021 ttcagcgcct tggagttgga tttcttactg aggcgagtat tgtagagata gaccacaaac
    58081 ataatcgctg cagaaatgat tcctagagtt gcaccaagag gatcaatgac cgtttcttcc
    58141 cgactgagaa tcttttgaat ggtatctctt agaacatcga aaccgacata gaacatgatg
    58201 atagaagtga tcaagcttgc caaatcttca atcttccaat gaccaaaacg gtggtcacgg
    58261 tctgcaggct ggcgcgccat ccgaatcccg attaagaggg ccacatttcc aatgatgtcc
    58321 gatacgttat taaaaccatc ggccaccaaa ctggatgaat gaaggagatg accagctgct
    58381 aatttagctg cagacaagat caaataggtc gaaatactga taatggctcc acgctcagct
    58441 aacttgagat ttgagataga ttgcttcatt caacttcctt tctagtcaca tagcattcta
    58501 ccattatact ggttttcaag gaaaaattca aataaggcag tttttactct ttgaaaactc
    58561 cccaaaattt ggtataatgg tactactcaa gggagtagct ggcagaaacc tgtgatagtg
    58621 tcgtcattcc gaattttatg ctgaaaagta tgctttccgg ccctatctta aacagcgaga
    58681 cttgttatga ttaacaggtc tctttttgtt tgtcgtgaaa agatatgaca gaaagagagt
    58741 ctgttttgca cacaatgtca aggaggagac acatgtcaaa agaacaaaaa cgccaagcgt
    58801 tttatactca gagccctgaa gaggtcttgc aggctgtgga tgcgaccgag caaggtttgt
    58861 catcaagtga ggcggaaaag cgccttgccg aatttgggca caatgaactc gaagaaggcg
    58921 agaaacgatc aatcttggtc aaattcatcg agcaatttaa ggatttgatg attatcatcc
    58981 tagttgcggc agcaatcttg tcagtcgtga cttctggtgg ggaagatatc gcagatgcca
    59041 ttatcatcct agctgtggtt atcatcaacg ctgcctttgg tgtttaccaa gaaggaaaag
    59101 ctgaagaagc tattgaagcc ctcaaatcca tgtctagtcc agttgcccgc gttcttcgtg
    59161 atggacacat ggcagagatt gactctaaag aattggtacc aggcgatatc gttgcccttg
    59221 aagcaggtga cgtggtacca gcggacctac gtttgataga agccaactct cttaaaattg
    59281 aagaagcagc cttgacaggt gaatctgtac cagtcgaaaa agacttgtca gtcgagcttg
    59341 cgacagatgc tggtattggt gaccgtgtca acatggcctt ccaaaactca aacgtaacct
    59401 atggtcgtgg gatgggtgtt gttgtcaata caggtatgta cactgaagtt ggtcatattg
    59461 ctggtatgct tcaagatgcg gatgagactg atacaccact caaacaaaac ttgaacaacc
    59521 tttctaaggt cttgacctat gctatcttgg tcattgccct tgttactttt gtagtgggtg
    59581 tcttcattca agggaaaaat ccacttggtg agttgttgac ttctgttgcc cttgccgttg
    59641 cagccattcc agaaggactt cctgctatcg ttaccatcgt tctttctctt ggtactcaag
    59701 ttttggccaa acgacattcc atcgttcgta agttgccagc agttgaaaca cttggttcaa
    59761 ctgaaatcat cgcttctgat aagactggta cgctgactat gaacaagatg acagtcgaaa
    59821 aagtcttcta cgatgcggtt ctacatgact cagctgatga tattgaacta ggtcttgaaa
    59881 tgccactact tcgttcagtt gtcttggcca atgatacgaa aatcgatgtg gaaggtaact
    59941 tgattggtga cccaaccgaa acagccttta tccaatatgc cttggacaag ggctatgatg
    60001 tcaaaggttt cttagagaaa tatcctcgtg tagctgaatt gccatttgac tctgaccgta
    60061 agctcatgtc aacagttcac ccattgccag atggtcgttt ccttgtagca gtcaagggtg
    60121 cgccagacca actcttaaaa cgttgtcttc ttcgtgataa ggctggggat attgctccga
    60181 ttgatgagaa ggttacaaat ctcattcata caaacaattc tgaaatggct catcaagcct
    60241 tgcgtgtcct tgcaggtgct tataagatta tcgatagtat tccagaaaat ctcacttctg
    60301 aagagcttga aaatgattta atttttactg gtttgattgg gatgattgac cctgaacgtc
    60361 ctgaagccgc tgaggctgtt cgtgtggcta aggaagcggg aatccgtcca attatgatta
    60421 caggtgacca ccaagacaca gcggaagcca ttgccaaacg tttgggaatc attgacgcaa
    60481 acgatacaga aggtcacgtt ttaactggtg ctgaactcaa tgaactgtca gatgaagaat
    60541 ttgaaaaagt cgttggtcaa tactctgttt atgcccgtgt gtctccagaa cacaaggttc
    60601 gtatcgtcaa ggcttggcaa aaacaaggta aagtcgttgc catgacaggt gacggtgtca
    60661 atgacgcgcc agctctgaaa acagccgata tcggtatcgg tatgggaatc actggtacag
    60721 aggtttctaa gggggcttct gatatgattc ttgcagatga taactttgcg actattatcg
    60781 tcgcagtgga agaaggacgt aaggtcttct caaacattca aaagactatt cagtacctac
    60841 tttctgctaa tactgctgaa gtattaacca tcttcctatc aaccttgttt ggttgggatg
    60901 tcttacagcc ggttcatctt ttgtggatca acttggtaac ggataccttc ccagctatcg
    60961 ctcttggtgt tgaacctgca gaacctggcg tcatgaatca taaaccacgt ggacgcaagg
    61021 caagcttctt ctcaggtggt gttttgagtt ctatcattta tcaaggtgta ctccaagcag
    61081 ctcttgttat gagtgtttat ggccttgcga ttgcttaccc agttcatgtg ggtgacaatc
    61141 atgctattca tgcagatgcc ctaacgatgg cctttgcaac ccttggtttg attcagctct
    61201 tccatgccta caatgtcaag tctgtctacc aatccatctt gacagttggc ccattcaagt
    61261 ctaagacctt taactggtcc atcttggtat cctttatcct tctcatggca acaatcgttg
    61321 tagaaccgct tgaaggaatc ttccacgtaa ccaaactaga cttgtcacaa tggggaattg
    61381 ttatggctgg aagcttctca atgattatca tcgtcgaaat cgttaagttt attcaacgca
    61441 aactcggttt tgacaagaat gcgatttaag ggaggtttcg gatgtcagca tatcaattac
    61501 cgaccgtatg gcaggatgaa gctagtaatc aaggagcttt tacggggcta aacagaccaa
    61561 cagcaggtgc ccgtttcgaa caaaacttgc caaaaggaga acaagctttt cagctttatt
    61621 cactgggaac accaaatggt gtgaaggtta ctatcttatt ggaagaatta ctagaagctg
    61681 gttttaagga agcggcttac gacttgtata agattgctat catggatggg gatcaattcg
    61741 gctcagactt tgtgaagctc aatccaaatt ccaagattcc agccttattg gaccagtcag
    61801 gtactgaaag cgtaagagtc tttgagtctg ctcatattct tctttacctt gctgagaaat
    61861 ttggagcctt tttaccaagt aatcctgtgg aaaaggtaga agttttgaat tggctattct
    61921 ggcaagcagg tgcagcacct tttctaggtg ggggatttgg acatttcttc aattatgctc
    61981 ctgaaaaatt ggaatatcct attaaccgtt ttacgatgga agtgaaacgc cagttggatt
    62041 tattggataa ggaattggct cagaaacctt atattgcagg caatgactat acgattgcag
    62101 atattgctat ctggtcttgg tatggacagt tagttcaagg aaatctttac caaggttctg
    62161 caaaattctt ggatgcctca agttatcaaa atctagtaaa atgggcagaa aaaattgcca
    62221 atcgtccagc tgttaagcgt ggattggaag taacttatac agaaattaaa tagcaaaaaa
    62281 gtcatcttcg gatggctttt tgctacagtt gagggaaagg gtttgcagtt atcggctttt
    62341 gtgctataat tgctataata tagtaaaaag caagaggagt gtgaggaagt ggaaaagaaa
    62401 attgtgaagg atatcttatt tttatctcaa gtgtctcagc cggcaagtca ggaggacctt
    62461 tatcttgcca gagatttgca ggatacactc ttagcaaatc gtgatacctg tgttggtcta
    62521 gctgccaata tgattggggt gcagaagcgc gtgattatct ttaatcttgg cttggttccc
    62581 gtggtcatgt ttaacccagt gcttctgtcc tttgaaggat cttatgaggc agaagaaggc
    62641 tgtttgtcct tggtaggtgt gagatcaact aagcgttatg aaaccataag gcttgcctat
    62701 cgtgacagca agtggcagga acagaccatt accttgacag gcttcccagc tcagatttgc
    62761 cagcatgagc tggatcactt ggaaggacga atcatttagg aggaaagcaa atgaaacgaa
    62821 tagtctttga acttattttt atcgcaacga cctggtatat ctttttaccg ccccttaacc
    62881 tgaccagctg ggaatttctc ttcttcctct gtgggcattt gttagttgtg gcaatcttat
    62941 ttggctttgg caaggggata aaccttgtca aaacggttca tgtgcgccac ggtaaggcgg
    63001 aagctgcctt aaatcttgag ggtttcaaaa tcaatcggtt agggaaaatt ctgttagctt
    63061 cgattggagg aattcttctc ttggcagctt tggtttcctt ggtaacttcc agcatgtttc
    63121 aggctaaaaa ttatgccaat gtagtcacgg ttacggaaaa agactttact gaatttccta
    63181 ggagtgacac cagtaaggtt cctatcctag atagaagtac tgctgaaaaa attggagacc
    63241 gctacttggg ttccctaacc gataaggtgt cgcaatacgt agcggcagat acctataccc
    63301 aattgacaat tgatgggaaa ccttatcggg tcacaccact agaatatgca gaccctatca
    63361 aatggtttaa caatcaagcc aagggaatcg gtgagtatat caaggtggac atggtaactg
    63421 gaaatgcgga tttggtggac ttgaagacac caatcaagta ttcagactcg gagtatttta
    63481 accgtgatgt caaacgtcac ctgcgcttga agtacccgac caaaatcttt aaaactccat
    63541 cttttgaggt ggacgatgag ggcaatcctt tctatgtagc aacggtttac caaaagcaat
    63601 ttggacttgc tgttcctcgt cctgcttcag tcattatctt ggatgctaca aatggagaaa
    63661 ccaaggaata cagcttatca gatgttccag aatgggtgga caggatctat ccagcagagg
    63721 aaaccattga gcaaatcaac tacaacggca agtacaagga cggtttcttg aatgccatga
    63781 tttccaagaa aaacgtgacc cagactacca agggctataa ttacttgtct atcggtaatg
    63841 acatctatct ctacacaggt gtgacgtcgg ctaatgcgga tgagagtaat cttggtttca
    63901 tccttgaaaa tatgcgaaca ggagaaatca ctaagtatag cttggcttct gcgacagaag
    63961 aatcagcccg tgaatcagca gaaggtgctg ttcaggagaa atcctacaaa gcaaccttcc
    64021 caatcctcat caacctcaat gacaagcctc tctacatcat gggcttgaag gacaatgctg
    64081 gcttggtcaa agagtacgcc ctggtagacg cagtcgagta ccaaaatgtt atcgttgcta
    64141 ctacagtgga agagatgctc agcaagtatg ccaataaaaa cgaccttgaa attgacaatg
    64201 caacgacaga aagcatcaat ggagtagtag cagacctcaa atcagctgtt atcaagggag
    64261 acactgtcta cttctttaaa gttgatggca agatatacaa ggtcaaggct tcagtatccg
    64321 atgaccttcc ttaccttgaa aatggtaaaa ccttcgaagg tcaagtagga aaagacaatt
    64381 atctcaagac ctttaagcta cggtaaaaat aggttttttt cagaaagtat atgttataat
    64441 aaggtaaatt aagcctaatg gaggacaaag aaatgggttt ttatttgatg gttgcttcaa
    64501 tgctactcgg actgctcgct ttgaagatag gtttttctca gttcaaggag aaaaaagata
    64561 aattcttatc tatcttaaca agtttagctg gcacagccct tgttctcgtg gctgtctggt
    64621 taggatggcc caaataaata gaaaaacctc ggtcaaaccg aggtttttca aatgaatttc
    64681 ttggttgtgg ctaaaaaata tgctacacta tcaatatgaa aattttaatc ccaacagcaa
    64741 aagaaatgaa cacagacttg ccaagtatcg aggcaatttc tttaaaacca gaaagtcagg
    64801 ccgtgcttga tgccttggct ctctattctg ccagtcaatt ggagagtttc tacaaggtat
    64861 cagctgagaa agcggcggaa gaatttcaaa atatccaagc tttgaaaagg caaactactc
    64921 aacactatcc agccttgaaa ctttttgatg ggcttatgta ccgcaacatt aagagagata
    64981 agctgaccga ggcggaacaa gattatcttg aaaatcatgt cttcattacc tcggctttgt
    65041 acggtgttgt tccagccttg tcacccatgg ctcctcaccg tttggatttt ttgatgaaat
    65101 taaaagttgc tggtaagact ttgaagagcc attggaaggc agtctatgat gaagctctga
    65161 agaaggaaga agtgattttc tctctcttgt catcagagtt tgagactgta ttttctaagg
    65221 aaatcagagc aaagatggtg accttcaaat tcatggagga tagaggcggt cagctgaaga
    65281 ttcactcaac tatctccaag aaagcgcgcg gggcctttct aacagcttta atagaaaatc
    65341 aagtacaaac tgtgggggaa gcacgtcgct tgaactttgc tggatttgtt taccgagaag
    65401 atttgtcaca accacaggga ttggtttttg taaaggaagt atagaaatca gatattttgt
    65461 atttggcaat atgtatcatc agctatttta gtttataaat gagaaaaaag cgaacaagct
    65521 agtcttctga gcgtcagaaa agtagtcttg ttcgctttta ttatgttata ctcttcgaaa
    65581 atctcttcaa gccatgtcag tgtcgcctta ccgtatatat gttactgact tcgtcagttc
    65641 tatctacaac ctcaaagcag tgctttgagt agcttgcggc tagcttccta gtttgctttt
    65701 tgatttttat tgagtgttag ttacttgata gcttcgacca agtcttgagc ttgtttttca
    65761 agtgagttta ggactgtttc ttcaagaacc aattttccgt ctgcccaggc agagtcatta
    65821 acacgtgcag cagtgaaatc accaacgcct tgtgtacgga taaatggcaa gaggtctttg
    65881 tagatagcga aaagttgatc gtgccctgca ttggctacag atgatactgt gacaaacttg
    65941 tcttgaaggg cagaaacgcc acgtgtatca gacaagtcaa gggcacgaga tagccagtca
    66001 agcaagtttt tcactgtacc agggatagag aagttgtaga ctggagagaa aatccagata
    66061 gcatccgcaa cgagaactgc ttcacgagca gcagctacag ctggatgtgt tggaacttcc
    66121 aaatcttggc tgaagagagg aagggctgaa taatcaaggt agctaacttc cgctttacca
    66181 gcaagtgctt tctcagcttc gagcgccatt tggtggttga aagaaccttg acgtagtgat
    66241 ccgacgataa ataatacttt tttagacatg atgtgtctcc tttttcttaa aatttcgaga
    66301 actctctcgt tgttataaaa tatattacaa caaaacaaaa gaacttgcaa gaaatttgct
    66361 cagagtttat ttgaaaattg taaaatttcg gaaactctag tcatttgtga ctgaaaaagt
    66421 aagaactgtt tcactttcaa aatcggtttt gtttgaaggg agaaatagaa ttggttgctc
    66481 aaagcacagc tttgaggttg cagataaagc tgacatggtt tgaagagatt ttcgaagagt
    66541 attcttatat tcacttgtaa taaagcttaa tagatggtaa aatagacgag tgaaaaaaag
    66601 acaaaacaaa gcaaaaaata atctactgtg gcagtatggt ctagggatga cgattttgtt
    66661 tgtggttatc agtgcttcct ttctgtatat ggtttctctt agcatgaaac cctatcaaac
    66721 agctaaaagt gaaggagaaa aattagctca gcagtatgca ggattagagc aggctgatca
    66781 ggttgattta tacaatggct tggaatctta ttacagcgtt cttggtcgta ataaacagca
    66841 agaagcgctt gctgtcctga ttggtaaaga tgaccataag atttacgttt atcagctaaa
    66901 tcagggtatt tcacaagaaa aagcagaaac ggtttctaag gaaaagggag ctggcgagat
    66961 tgacaagata acctttggtc gttatcaaga caagccaatc tgggaagtta agtcaggatc
    67021 tgatttttat ctagtagatt ttgaaacagg agcattggtc aataaggagg gcctatgaaa
    67081 ctatccaacc gtgttttaga aatggaagaa agtgtgactt tggctgctgg agcccgtgcc
    67141 aaagcactga aggctgaggg tagagatatt ttgtctctaa ccttgggtga gccagatttc
    67201 actactccca aaaatatcca agatgccgcc attgcatcga ttcgagatgg gcgtgcttct
    67261 ttttatacag taacctcagg tctgccagag cttaaggcgg tggtcaatag ctactttgag
    67321 cgcttttacg gctattctgt agcgtcaaat caagtgacag tcgctgcggg agccaaatat
    67381 tctctctata ccttctttat ggctgtggtc aatccaggtg atgaagtcat catcccaacc
    67441 ccatactggg ttagctatgg agatcaggtc aagatggcag aaggcgttcc cgtctttgtt
    67501 tctgctaagg aagacaatca ctttaaggtg accgtagagc agttagaagc agctcgcact
    67561 gacaagacca aggttttggt gctgaattcg ccatctaatc ccacaggtat gatttacacc
    67621 cgtgaggaac tcttggcaat tggaaactgg gctgtagaaa atgatattct catcctagca
    67681 gatgatatct atggccgctt ggtttataat ggtcatgagt tcacaccgat ttctagccta
    67741 tcggaagcga ttcgcaagca aacagtggtc atcaatggtg tgtctaaaac ctatgccatg
    67801 actggttggc ggattggtta tgccgttgga gaagcagaca ttatcgctgc catgtccaag
    67861 attgcaggtc aaacaacttc gaatccgtca gcagtagccc aatatgcagc agttgaggct
    67921 ctatcaggtg agcaagatac tgtagaaagc atgcgtcagg cctttgagga acgtcttaat
    67981 accatctatc ccctccttgc agaggtgcca ggatttgaag tggtcaaacc acaaggggcc
    68041 ttctatctct tcccaaatgt caaaaaggcc atggagatga aaggctacac ggatgtgaca
    68101 gactttacaa ctgctatctt agaagaagcc gaagtggcct tggtaacagg agctggattt
    68161 ggagcaccag aaaatgtccg cctcagctat gcgacagacc tagacacgct taaagaagca
    68221 gtcgaacgct tgaaagcatt tatgggtagt gagaatgatt gatcattttg agattaaggt
    68281 aaaggattta caaatttcag aaggatttta taggagtttt ctcgctcctt tggactataa
    68341 attggctttt aagactagtt ctctaattag ttttctatcc ccgaacagcc ctcatcctgg
    68401 tggtgatttt tggctgactc agggaacaca agatcctgtt cattttgctt ttttagcaga
    68461 aaataaggaa gaagttcagg cttgttatga ggctggctta gaggcaggtg ggcgagacaa
    68521 tggggttcct ggttatcgaa gtgagcatcc gatttattat gctgctttta tgattgacct
    68581 ggatgggaac aatatagagg tggtttgcca taaagaataa aaaagaaaag gaaaaataat
    68641 gacaaaacgt gtaacgatta ttgatgtaaa agactatgtt ggtcaggaag tgacgattgg
    68701 cgcttgggtt gccaacaaat caggaaaagg gaaaatcgct ttcttacaat tgcgtgatgg
    68761 aacagccttc tttcaaggtg tggcttttaa accaaacttt gtcgaaaaat ttggtgaaga
    68821 agtgggactt gagaagtttg atgttatcaa acgcttgagc caagaaacgt ctgtttatgt
    68881 gacaggtatt gtcaaagagg acgaacgttc taaatttggc tatgagttgg acatcacaga
    68941 catcgaagtg atcggtgaat ctcaagacta cccaatcaca ccaaaagaac acggaacaga
    69001 ctttttgatg gataaccgtc acttgtggct acgctctcgt aagcaagtag ctgtgttgca
    69061 aatccgtaac gctattatct atgcaactta tgagttcttt gacaagaacg gttttatgaa
    69121 gtttgacagc ccaattcttt caggaaatgc ggcagaagat tctacagaac tctttgaaac
    69181 tgactacttc ggaacgccag cctacttgag ccaatcaggt cagctttacc tagaagcagg
    69241 ggctatggct cttggtcgtg tctttgactt tggtccagtt ttccgtgctg aaaaatcaaa
    69301 aacacgccgt cacttgactg agttctggat gatggatgct gagtactcat acttgacaca
    69361 tgatgagtcg cttgacttgc aagaagctta tgtgaaagct cttctacaag gtgttcttga
    69421 ccgcgcgcct caagccttgg aaaccttgga acgtgataca gaactcttga aacgctacat
    69481 tgcagagcca ttcaaacgta tcacttacga tcaagccatt gacctcttgc aagagcatga
    69541 aaatgatgaa gatgctgact acgagcatct tgagcatggt gatgactttg ggtcaccaca
    69601 cgaaacttgg atttcaaacc actttggtgt gccaacattt gtcaagaact atccagcagc
    69661 catcaaggcc ttctacatga aaccagttcc tggaaatcca gagcgcgtgc tttgtgcaga
    69721 cttgcttgct ccagaaggct atggagaaat tatcggtggg tctatgcgtg aggaagatta
    69781 cgatgccctt gtcgctaaga tggatgaact tggcatggat cgtacagaat atgaattcta
    69841 ccttgacctt cgtaaatacg gtacagttcc acacggagga tttggtatcg gtatcgaacg
    69901 tatggtaacc ttcgcagcag gaacaaaaca tatccgtgaa gctattccat tcccacgtat
    69961 gttgcaccgt atcaaaccat aagaacagga aaacgcccat gtggcgtttt ttcgaattct
    70021 agtgttttta cttgcgtaaa aaaatttttt ctagtataat agtttattgt gagcgaacct
    70081 cacttacccc ttgcaaagtc ttggggtcat tagaccaaaa ggaggaacat atcaatggct
    70141 aaatacgaaa ttctttatat cattcgtcca aacattgaag aagaagctaa aaacgctttg
    70201 gtagcacgtt ttgactctat tttgactgac aacggtgcaa ctgttgttga atcaaaaact
    70261 tgggaaaaac gtcgtcttgc atacgaaatc caagatttcc gtgaaggact ttaccacatc
    70321 gttaacgttg aagcaaatga cgatgcagct cttaaagagt ttgaccgtct ttcaaaaatc
    70381 aacgctgaca ttcttcgtca catgatcgtc aaaattgacg cgtaagaagg taaactatga
    70441 ttaacaatgt tgtacttgta gggcgtatga cacgtgacgc tgagttgcgt tataccccat
    70501 caaatgtagc agttgcgact tttactcttg cagtaaaccg tacatttaag agtcaaaatg
    70561 gtgaacgtga ggctgatttt ataaatgtcg ttatgtggcg ccaacaggct gaaaatcttg
    70621 ctaactgggc taaaaaaggc tcacttatcg gggtgacagg tcgtatccag actcgtagtt
    70681 acgataacca gcaaggacaa cgtgtctacg tgacagaggt cgtggctgag aatttccaaa
    70741 tgttggaaag ccgtagtgtg cgtgagggtc acacaggtgg agcttactct gcaccaactg
    70801 caaactattc agcacctaca aattcagtac cagacttttc acgtaatgaa aatccatttg
    70861 gagcaacaaa cccattggat atttcagatg atgatttacc attctaatgg acaactaaac
    70921 ttataaagga gaaaaaacat ggctcaacaa cgtcgtggcg gattcaaacg ccgtaaaaaa
    70981 gttgattaca tcgcagcaaa caaaattgaa tatgttgatt acaaagatac tgagcttctt
    71041 agccgtttcg tttcagaacg tgggaaaatc cttcctcgtc gtgtaacagg aacttcagct
    71101 aaaaaccaac gtaaagtaac aacagctatc aaacgcgctc gcgtaatggc tttgatgcct
    71161 ttcgtaaacg aagattaaag aaaagacccg gttgggtctc tttttcatga gcttgaaaac
    71221 aagaaagcga attaagaccc caatgggtct tttttcacaa actttgaaat gagagagcga
    71281 gttaggctca gtggctccct ttttacgagt ttgaaaacaa gagaacacat taaaagcacg
    71341 atggagcttt ttcataaata tgtctttttt ctcatccaag aaattaaaat aaaaatgata
    71401 aactttttga aaatagggga tttgcctact ttatgatata atggaaagag ttagattgaa
    71461 agaggtagtg agatggacgc gaaattaaga tacaaggcaa agaagatcaa gattgtcttt
    71521 tttgatattg atgatacatt gcggaattca aagacagggt ttattccaac tacaattccc
    71581 actgttttta aacagttgcg tgaaaaagga attttaacag gaatcgcctc tggacgtggc
    71641 atttttggtg ttgttccaga gattcgtgat ctcaagcctg acttttttgt aactttgaat
    71701 ggggcttata tcgaagataa aaaaggtcag gtcatttatc agcatcagat tgaaaagtca
    71761 gatgttgagg agtatatctc ttgggctaag caagaaggaa ttgagtatgg cttggttggg
    71821 agtcatgatg ccaagttgtc gattcgcacc gatatgatga gtgaagctat caatccaatt
    71881 tatcccgact tagatgtaga tcccgatttc catgaaaaag aagatatcta tcagatgtgg
    71941 acttttgaag ataagggaga tgacttgcac ttgcctgaca gtctctcaga caaacttcgc
    72001 atggttcgtt ggcatcaaca ttcgtctgat attgtgccga tttcaggctc caaagcgacg
    72061 ggggtggaaa aggttgtgga acaccttggc ttgaaaccag agaaggtcat ggtttttgga
    72121 gatggtctca acgacttgga actctttgat tatgctggaa tcagcgttgc catgggaatt
    72181 tctcatgata aaatcaaaga aaaagcagat tatattacaa aaacattaga agaagatggc
    72241 atttttgatg ccttagaagt atttggtatg gtagaaaaag aattgcattt tccacaagta
    72301 gacattgaaa cagtagaagg tcctcttgcg actattaaga ccaatcacgg agacttacgt
    72361 atcaagcttt tccctgaaca tgctcctaaa acagtggcta actttgtatc tctttcaaaa
    72421 gatggctact atgatggtgt cattttccac cgtattatca aggactttat gatccaaggt
    72481 ggagacccaa ctggaactgg tatgggtggc gagtcaatct acggcgaatc ttttgaggat
    72541 gaattctcag aagagcttta caatatccgt ggtgctcttt ccatggcaaa tgctggtcca
    72601 aataccaacg gcagccagtt ctttatcgtg caaaaccaac acctacctta ttctaagaaa
    72661 gaaattactc gtggtggttg gccagaaccg attgcagaaa tctatgccaa tcaaggtggg
    72721 acacctcacc tagaccgccg tcacacggtt tttggtcagt tagctgatga agcatcttac
    72781 gctgtcttgg atgccattgc tgctgttgag acaggagcta tggacaagcc agttgaagat
    72841 gttgtaattg aaactattga aatcgaggac taagatgaaa atcggtgata agctaaaggg
    72901 gcgtattaca gggattcagc cctacggtgc ctttgttgag ttagagacgg gtgatacggg
    72961 gctgattcac atttcagaga ttcggacagg atttattgaa aatattcatg aaaccttgaa
    73021 agtcgatgaa gaggttcaag ttcaggtagt ggatttagat gaatttacgg ggaaagccag
    73081 tctttctatc cgcactttgg aggaaaaaaa gtaccagttt cctagacgga gacgcttttc
    73141 aagcgaccgc tttaactacg gttttgctcc ctttcgacga atgctaccaa tctggactgg
    73201 agaagccctg caccatttaa aaaagaagaa gtagtttaga gttttatatt ttttgtaatg
    73261 ttaatatgaa tttgctataa taggaccatg gaagaagaac aattattaaa atcaggagag
    73321 cgcattaacc agctcttttc gacagatatc aaaatcattc aaaatagaga ggtttttagc
    73381 tattcggtgg atagtgttct cttatcacga tttccacgtt ttcctaagaa ggggttgatt
    73441 gtggatttct gtgctgggaa tggagcagtg gggctttttg ctagcactcg tactcaagca
    73501 cagatattgt ctgttgagat tcaggagcgt ttggcggata tggctgaacg ctctgtccgt
    73561 ttgaatggat tagaggagca aatgcaggtc atctgcgatg atttgaaaaa tatgcctgct
    73621 cacattcagg ggagtaaggt ggatatgatt ttgtgtaatc caccctattt caaggtggat
    73681 ccttattcta atctgaacga gagtgaacat tatctcttgg ctcgacacga aatcacgact
    73741 aatttggaag aaatctgtcg tagtgcccag agtattctca agtctaatgg gcgtttggcc
    73801 atggttcatc gtcctgatcg acttctggat attttggata cgttaaaacg acataatcta
    73861 gcccctaagc gcctgcagtt tgtttatcca aaaagagaaa aggaagccaa tatgcttttg
    73921 attgaggcga tcaaggatgg ctcaacaagt ggctttaagg tcttacctcc tttgattgtc
    73981 cacaatgatg atggctctta tacgcccgaa atcgaagaga tttattatgg atcataaggc
    74041 ttatatgtac gttctggagt gtcgtgatgg atcctactat ataggctata cgactgatat
    74101 gagaagacgc ctcgctatcc acaatagtgg gaagggagcc aaatatacac gagcacgctt
    74161 gccagtcaaa cttatctatg ctcaaggttt tgccagtaag gaagaagcca tgtcggctga
    74221 agctcttctc aagcgtaaga agaggccaca gaaggaagaa tttttatctg aaaatcaaga
    74281 tagaaattta ctcagttatt ttgaagaatc gtggggagtc ctttgactcc tcttaccttt
    74341 gtcaaaaagc gaaaaaaatg ctacaataaa gaaaataaac aaaatgaggt attatcatgt
    74401 ccaagattct agtatttggt caccaaaatc cagactcaga tgccatcggg tcatctgtag
    74461 cttttgccta ccttgcaaaa gaagcttacg gtttggatac ggaagctgtt gcccttggaa
    74521 ctccaaatga agaaacagcc tttgtcttga actattttgg tgtggaagca ccacgtatta
    74581 tcacttctgc caaagcagag ggggcagagc aagttatctt gactgaccac aatgaattcc
    74641 aacaatctgt atcagatatc gctgaagtag aagtttatgg tgttgtagac caccaccgtg
    74701 tggctaactt tgaaactgca agcccacttt acatgcgttt ggagccagtt ggatcagcgt
    74761 cttcaatcgt ttaccgtatg ttcaaagaac atggtgtagc tgtgcctaaa gagattgcag
    74821 gtttgatgct ttcaggtttg atttcagata cccttctttt gaaatcacca acaacacacc
    74881 caacagataa aatcattgct cctgaattgg ctgaattggc tggtgtgaac ttggaagaat
    74941 atggtttagc aatgttgaaa gctggtacca acttggctag caaatctgct gaagaattga
    75001 ttgacatcga tgctaagact tttgaactca acggaaataa tgtccgtgtt gcccaagtga
    75061 acacagttga catcgctgaa gttttggaac gccaagcaga aattgaagct gcaatgcaag
    75121 ctgccaacga atcaaacggc tactctgact ttgtcttgat gattacagat atcgtcaact
    75181 caaactcaga aatcttggct cttggtgcca atatggacaa ggtcgaagcg gctttcaact
    75241 tcaaacttga aaacaatcat gccttccttg ctggtgccgt ttcacgtaag aaacaagtgg
    75301 tacctcaatt gactgaaagc tttaatgcgt aagattttgg gtgtcagctc aaaatcggaa
    75361 agtctagttt gccttatatc gaaaggagtt tcggctcctt ttttctagga gtgaagtatg
    75421 ttagaaaatg gcgatttgat ttttgtgaga gatgggtcag acatgggaca ggccatccag
    75481 acttccacag gtaactatag ccatgttgcc atttatttgg atgggatgat ttatcatgct
    75541 agtggacagg ctggtgttgt ctgtcaagaa ccggcagact tctttgagtc caatcattta
    75601 tacgacctct atgtttaccc agaaatggat atccagtcgg tgaaggaaag agcttgcaaa
    75661 catcttggag caccctacaa tgcttctttc tatccagatg cagctggttt ctactgctcc
    75721 cagtatatag cagaaatcct acctattttt gaaactattc ctatgaaatt tggagatggg
    75781 gagcaggaga ttagtgattt ttggagggag tattacatag aactaggtct gcctgttcct
    75841 ctgaaccaag ctggtaccaa tcctagtcag ttggcagcat cgcctctgtt acaatgtaaa
    75901 gaaagggatc ttcatgattc agatttttaa tccatctcgt ttgacgagac agccattttt
    75961 ggagaattga tccgctatct ggaccagtat gaggatgtga ttctacggga aattaaggct
    76021 caatttccag atgttgcagt tgataaactc atggaagagt atataaaggc aggcttgatt
    76081 ctacgtgaaa ataagcgcta ttacctcaat tttcctacgc ttgaatcact tgatagtctt
    76141 gaactggatc aagagatttt tgtcagagaa gctagtccgg tctatcaagc cttgttggag
    76201 cagagttttg agacggaatt gcgcaatcaa atcaatgcag ctattttagt tgaaaagacg
    76261 gactttgcgc gcattaaaat gaccctgtcc aattattttt acaaggtcaa acagcagtat
    76321 cctttgacag aaaaacagca ggagctctat gacattttag gagatgttaa tcctgagtat
    76381 gccctcaagt atatgacggc ttttttgttg aaatttctca aaaaagacca gcttatgcag
    76441 aaatgccgtg atatctttgt ggacagtttg gttgtcttag gctatattgt gcaaaatgaa
    76501 gatagaaagt atgagttggc tatcgatttt gataaggaga gattaacttt ctacttagcg
    76561 tgatttcttg tttctgagca cattgtttga ctttccttag tattcggtat aaactatatg
    76621 taaccggtaa cacatatcgg aataaactaa aggagacaat catatgtcac ttgaaaacaa
    76681 attggaacaa gcaacaggcg ctgtcaaaga aggttttggt aaagttactg gagacagcaa
    76741 gacagaactt gaaggagctg ttgaaaaaac agttgctaag gcaaaagacg ttgtagaaga
    76801 cgcaaaaggt gctgtagaag gtgccgttga aggtttgaaa aacgttttta ctaaataata
    76861 ggaaaaaatc aagggtttca ttttcccttg attttttcta ttcttataaa taattttctg
    76921 cgacggctgt atctcctggg taggattctt tcttgccctg gatgatttgg taacaatcga
    76981 ctcccttacc cgcaataata actgcatcta attcgtgatt tgtgatagcc attgccgcct
    77041 tgatggcttc ttggcgatcc gcaatctttt caacaggatg attgatgtag ctactaattt
    77101 catctgcaat ggccattggg tcttcatagt tagggtcatc agcagtcaga aagacttgaa
    77161 tctcagggtg ttgattgagg aggaggccaa agtccttacg acgactttct cccttgtttc
    77221 ctgttgatcc cagaaccaga gcaatctttc cggtttgatg agtttcaacc acattgatga
    77281 gttttttcag actatcccca ttgtgggcat agtcgatgaa gaccttggct ccatttttct
    77341 gagtgaggac ttccatacga ccaggaacgc gggttgcagc gatgcctttt ttgatgtcct
    77401 caagacttgc tccgagacgg agacaagcaa gtccagcagc aactgcattt tcttggttga
    77461 agttgccaat gagttgaata tcataatctc cagcgagttt acccgtagct gaaaagctaa
    77521 aggctttgga attctcgatt tggttatcaa attggctacc atagaaatca tggtcttgat
    77581 cttcaacctg ttctttcaag actgagaagt ggtccatgtc actgttaatg atgactgctc
    77641 ggctcttttc catcaagaga cgcttgtggt agaaatagtc ttcaaagcta gggtgttcaa
    77701 tcgggccgat atggtctggg ctgatattta ggaaaactcc cacatcaaag gttagaccat
    77761 agacacgttt gaccagatag gcttgactgg agacttccat gatgaggtgg gtacggtcat
    77821 tttgcacagc ctgattcatc atgtcaaaga ggtcaatact ctcaggggtt gtcaacgctg
    77881 acttaaagaa agtctcgcca tcaagagttg tgttcatggt cgacaacata gcaggtctat
    77941 gcccttgaga taagatgtta taggcgaaat aggctgctgt tgtcttaccc ttagtaccag
    78001 taaaggcaag gagtttgagt ttttcctgtg gattaccata gaactccatg gcaatcaaac
    78061 tcatggcttt ctttatatcg ttcacaatga tgacagggat accgacttcg tagtcctttt
    78121 cagctacata ccaagctaat ccttgtgtta tagcagaaag aaggtattct tttttaaagg
    78181 cagcgccttt tgcgaaaaaa agagtgtctt ctgttacttt tcggctgtcg tagctgatgc
    78241 tatcaaaaat aactttgctg tagttgtagt ggtaatgacc ttggtcaata atttcgcgaa
    78301 aaaggccatc tttctttaaa atatctaata cggtttcaat cttaatcata ctttctattg
    78361 taaaccgaaa gtcgtaaatt tacaagtaac aaggaaaagt ttataatgga agataaggag
    78421 tttttcctag ctatcaaaat tgaatgagga atctatgtcg cacgaaaaca atcaccagca
    78481 ggcccagatg ttacggggga ctgcttggct aacggctagt aactttatca gtcgcctact
    78541 cggggctgtt tacattatcc cttggtacat ctggatgggg gcttatgcag ctaaggcaaa
    78601 tggtctcttt accatgggtt acaatatcta tgcttggttc ttgttggttt caacagcggg
    78661 gattccagtt gcggtggcca agcaagttgc caagtataat accatgcgag aagaagagca
    78721 tagctttgcc ctgattcgga gtttcttagg ctttatgaca ggactaggcc tggtttttgc
    78781 tttagtcttg tatgtctttg ctccttggct agcagacttg tctggcgtgg gcaaagactt
    78841 gatcccaatc atgcaaagct tggcttgggg agtcttgatt ttcccgtcta tgagtgttat
    78901 ccgaggattt ttccaaggga tgaataacct caaaccctat gccatgagcc aaattgctga
    78961 gcaggtcatt cgtgttatct ggatgctcct agcaaccttt atcattatga agctcggttc
    79021 aggagattat ctagcagccg ttacccaatc aacctttgct gcctttgtcg gtatggtagc
    79081 cagttttgca gtcttgattt atttccttgc ccaagaaggt tcactcaaaa gagtctttga
    79141 aacaggagat aagattaaca gtaagcgtct cttggttgat accattaagg aagccattcc
    79201 ttttatcctg acagggtctg ccatccagct cttccagatt ttggatcagc tgacctttat
    79261 caatagtatg agctggttta ccaactacag caatgaggac ttggttgtca tgttttctta
    79321 tttctcagcc aatcctaata aaatcacgat gattttgatt tctgtagggg tttcgattgg
    79381 gagtgttggt ttgccacttt tgacggaaaa ctatgtcaag ggggacttga aagcagcttc
    79441 tcgtctcgtt caggacagtc tcaccctact ctttatgttc ttgctaccag caacggttgg
    79501 agtggttatg gtaggagaac ctctatatac agtcttctat ggtaagccag atagtttggc
    79561 tctgggctta tttgtctttg cagttttgca gtctattatt ttaggcttgt acatggtctt
    79621 gtctccaatg cttcaggcca tgttccgcaa ccgcaaggcc gttctctatt ttatctatgg
    79681 ttctattgcc aagctagtct tgcaactacc taccatcgcc ctcttccaca gttatggtcc
    79741 tttgatttca acaaccattg ctctcatcat tcctaacgtc ttgatgtatc gggatatttg
    79801 taaagtaact ggtgtcaagc gcaaggtgat tttgaagcga actattttaa tcagtttgct
    79861 gaccctagtc atgtttctgt taataggaac catccagtgg ctgttaggat ttttcttcca
    79921 accaagtgga cgtttgtgga gcttctttta tgtagctctt gtcggtgcca tggggggtgg
    79981 actttatatg gttatgagtc tgcgtaccta tttattagat aaggtaatag gaaaagccca
    80041 agcagatcgc ctgcgagcaa aatttaagct ttcgtaaggt agcatttata aataaaagga
    80101 ataagttgaa caatctttta ggagaacttg aaacttattc cttttttagc gta
//
LOCUS       DAVLYR010000002        47616 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLYR010000002 DAVLYR010000000
VERSION     DAVLYR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.002"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            95..1120
                     /gene="trpS"
                     /locus_tag="VYO63_000103"
     CDS             95..1120
                     /gene="trpS"
                     /locus_tag="VYO63_000103"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1671092.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1272..2750
                     /gene="guaB"
                     /locus_tag="VYO63_000104"
     CDS             1272..2750
                     /gene="guaB"
                     /locus_tag="VYO63_000104"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1671093.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2801..3898)
                     /gene="recF"
                     /locus_tag="VYO63_000105"
     CDS             complement(2801..3898)
                     /gene="recF"
                     /locus_tag="VYO63_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1671094.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(3901..4269)
                     /gene="yaaA"
                     /locus_tag="VYO63_000106"
     CDS             complement(3901..4269)
                     /gene="yaaA"
                     /locus_tag="VYO63_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1671095.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4428..5678
                     /locus_tag="VYO63_000107"
     CDS             4428..5678
                     /locus_tag="VYO63_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1671096.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5675..6958
                     /locus_tag="VYO63_000108"
     CDS             5675..6958
                     /locus_tag="VYO63_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1671097.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGYVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTIDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7000..7821
                     /locus_tag="VYO63_000109"
     CDS             7000..7821
                     /locus_tag="VYO63_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1671098.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7832..8377
                     /gene="pgsA"
                     /locus_tag="VYO63_000110"
     CDS             7832..8377
                     /gene="pgsA"
                     /locus_tag="VYO63_000110"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1671099.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8374..9201
                     /locus_tag="VYO63_000111"
     CDS             8374..9201
                     /locus_tag="VYO63_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1671100.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9186..10025
                     /locus_tag="VYO63_000112"
     CDS             9186..10025
                     /locus_tag="VYO63_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1671101.1"
                     /translation="MGIALENVNFIYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10018..10812
                     /locus_tag="VYO63_000113"
     CDS             10018..10812
                     /locus_tag="VYO63_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1671102.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10871..11689
                     /gene="mreC"
                     /locus_tag="VYO63_000114"
     CDS             10871..11689
                     /gene="mreC"
                     /locus_tag="VYO63_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1671103.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11689..12183
                     /gene="mreD"
                     /locus_tag="VYO63_000115"
     CDS             11689..12183
                     /gene="mreD"
                     /locus_tag="VYO63_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1671104.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12277..13455
                     /locus_tag="VYO63_000116"
     CDS             12277..13455
                     /locus_tag="VYO63_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1671105.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13679..14458
                     /gene="rpsB"
                     /locus_tag="VYO63_000117"
     CDS             13679..14458
                     /gene="rpsB"
                     /locus_tag="VYO63_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1671106.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNK"
     gene            14524..15564
                     /locus_tag="VYO63_000118"
     CDS             14524..15564
                     /locus_tag="VYO63_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1671107.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15810..16133
                     /gene="tnpB"
                     /locus_tag="VYO63_000119"
     CDS             15810..16133
                     /gene="tnpB"
                     /locus_tag="VYO63_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1671108.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(16274..16561)
                     /locus_tag="VYO63_000120"
                     /pseudo
     CDS             complement(16274..16561)
                     /locus_tag="VYO63_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16552..16950
                     /locus_tag="VYO63_000121"
     CDS             16552..16950
                     /locus_tag="VYO63_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1671109.1"
                     /translation="MKAPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17147..18067)
                     /gene="cysK"
                     /locus_tag="VYO63_000122"
     CDS             complement(17147..18067)
                     /gene="cysK"
                     /locus_tag="VYO63_000122"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1671110.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18352..18987)
                     /locus_tag="VYO63_000123"
     CDS             complement(18352..18987)
                     /locus_tag="VYO63_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1671111.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19043..20341
                     /locus_tag="VYO63_000124"
     CDS             19043..20341
                     /locus_tag="VYO63_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1671112.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20338..21000
                     /locus_tag="VYO63_000125"
     CDS             20338..21000
                     /locus_tag="VYO63_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1671113.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21080..21628
                     /gene="raiA"
                     /locus_tag="VYO63_000126"
     CDS             21080..21628
                     /gene="raiA"
                     /locus_tag="VYO63_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1671114.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21764..23737
                     /locus_tag="VYO63_000127"
     CDS             21764..23737
                     /locus_tag="VYO63_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1671115.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23734..24186
                     /gene="rplI"
                     /locus_tag="VYO63_000128"
     CDS             23734..24186
                     /gene="rplI"
                     /locus_tag="VYO63_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1671116.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24230..25582
                     /gene="dnaB"
                     /locus_tag="VYO63_000129"
     CDS             24230..25582
                     /gene="dnaB"
                     /locus_tag="VYO63_000129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1671117.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25584..25850
                     /locus_tag="VYO63_000130"
     CDS             25584..25850
                     /locus_tag="VYO63_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671118.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            25939..27285
                     /gene="cbpD"
                     /locus_tag="VYO63_000131"
     CDS             25939..27285
                     /gene="cbpD"
                     /locus_tag="VYO63_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1671119.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27368..27470
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27541..27831
                     /locus_tag="VYO63_000132"
     CDS             27541..27831
                     /locus_tag="VYO63_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1671120.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQGADNVFSNVKINVGEILSIDEKLEKYTETTH"
     gene            27794..28552
                     /locus_tag="VYO63_000133"
     CDS             27794..28552
                     /locus_tag="VYO63_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671121.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWFNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            28590..29597
                     /locus_tag="VYO63_000134"
     CDS             28590..29597
                     /locus_tag="VYO63_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1671122.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29597..30325
                     /locus_tag="VYO63_000135"
     CDS             29597..30325
                     /locus_tag="VYO63_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671123.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPQGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30444..30902
                     /locus_tag="VYO63_000136"
     CDS             30444..30902
                     /locus_tag="VYO63_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1671124.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            30904..33336
                     /locus_tag="VYO63_000137"
     CDS             30904..33336
                     /locus_tag="VYO63_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1671125.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIATLRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            33657..34310
                     /gene="rr06"
                     /locus_tag="VYO63_000138"
     CDS             33657..34310
                     /gene="rr06"
                     /locus_tag="VYO63_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1671126.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDEITQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34307..35638
                     /gene="hk06"
                     /locus_tag="VYO63_000139"
     CDS             34307..35638
                     /gene="hk06"
                     /locus_tag="VYO63_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1671127.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            35721..36248
                     /locus_tag="VYO63_000140"
     CDS             35721..36248
                     /locus_tag="VYO63_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1671128.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36335..38374
                     /gene="pspC"
                     /locus_tag="VYO63_000141"
     CDS             36335..38374
                     /gene="pspC"
                     /locus_tag="VYO63_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV1671129.1"
                     /translation="MFASKNERKVHYSIRKFSVGVASVAVASLFMGSVVHATEREGST
                     PVATSSNKANKSQTEHMKAAEQVDEYINKMIQLDKRKHTQNLALNIKLSAIKTKYLRE
                     LNVLEEKSKKEELTSKTKKEIDAAFEQFNKDTLKPGEKVEEAQKKVEEAEKKAKDQKE
                     EDHRNYPTITYKTLELEIAESDVEVKKAELELVKEEAKGSRNEEKIKKAKAEVESKKA
                     EATKLEEIKTERKKAEEEAKRKAEAEEEVKDKLKKRTKRGALGEPATPDKKENDAKSS
                     DSSVGEETLPSPSLKSEKKVAEAEKKVAEAEKKAKDQKEEDRRNYPTNTYKTLELEIA
                     ESDVKVKEAELELVKEEAKEPQNEEKIKQAKAKVESKKAEATRLEKIKTDRKKAEEAK
                     RKVAEEDKVKEKPAEQPQPAPAPKPAPAPQPEKPAEQPKAEKPADQQAEEDYARRSEE
                     EYNRLTQQQPPKAEKPAPAPQPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNG
                     SWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGDMATGWLQN
                     NGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGSMATDWV
                     KDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(38604..39584)
                     /gene="dusB"
                     /locus_tag="VYO63_000142"
     CDS             complement(38604..39584)
                     /gene="dusB"
                     /locus_tag="VYO63_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1671130.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGTISQASTLAEIEALLQLEKA"
     gene            complement(39571..40443)
                     /gene="hslO"
                     /locus_tag="VYO63_000143"
     CDS             complement(39571..40443)
                     /gene="hslO"
                     /locus_tag="VYO63_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1671131.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            40608..42035
                     /locus_tag="VYO63_000144"
     CDS             40608..42035
                     /locus_tag="VYO63_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1671132.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSSLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKTERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            42194..43702
                     /gene="glpK"
                     /locus_tag="VYO63_000145"
     CDS             42194..43702
                     /gene="glpK"
                     /locus_tag="VYO63_000145"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1671133.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            43744..45570
                     /gene="glpO"
                     /locus_tag="VYO63_000146"
     CDS             43744..45570
                     /gene="glpO"
                     /locus_tag="VYO63_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1671134.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTSAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            45640..46344
                     /locus_tag="VYO63_000147"
     CDS             45640..46344
                     /locus_tag="VYO63_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1671135.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            46641..46844
                     /locus_tag="VYO63_000148"
     CDS             46641..46844
                     /locus_tag="VYO63_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671136.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(46931..47539)
                     /locus_tag="VYO63_000149"
     CDS             complement(46931..47539)
                     /locus_tag="VYO63_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671137.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT        14733 a         8121 c        10592 g        14170 t
ORIGIN      
        1 tctgtaaaaa gcaaggctga tttattttga taaattacgg ttatttcatt aaaaaaatgc
       61 tataattgaa aggactatat cgaaggagaa caaaatgact aaacccatta ttttaacagg
      121 agaccgtcca acaggaaaat tgcatattgg acattatgtt ggaagtctca aaaatcgagt
      181 attattacag gaagaggata agtatgatat gtttgtgttc ttggctgacc aacaagcctt
      241 gacagatcat gccaaagatc ctcaaaccat tgtagagtct atcggaaatg tggctttgga
      301 ttatcttgca gttggattgg atccaaataa gtcaactatt tttattcaaa gccagattcc
      361 agagttggct gagttgtcta tgtattatat gaatctagtt tcgttagcac gtttggagcg
      421 aaatccaaca gtcaagacag agatttctca gaaaggattt ggagaaagca ttccgacagg
      481 attcttggtc tatccaatcg ctcaagcagc tgatatcaca gctttcaagg ctaattatgt
      541 tcctgttggg acagatcaga aaccaatgat tgagcaaact cgtgaaattg ttcgttcttt
      601 taacaatgca tataactgtg atgtcttggt agagccggaa ggtatttatc cagaaaatga
      661 gagagcaggg cgtttgcctg gtttagatgg aaatgctaaa atgtctaaat cactaaataa
      721 tggtatttat ttagctgatg atgcggatac tttgcgtaaa aaagtaatga gtatgtatac
      781 agatccagat catatccgcg ttgaggatcc aggtaagatt gagggaaata tggttttcca
      841 ttatctagat gtttttggtc gtccagaaga tgctcaagaa attgctgata tgaaagaacg
      901 ttatcaacga ggtggtcttg gtgatgtgaa gaccaagcgt tatctacttg aaatattaga
      961 acgtgaactg ggtcctattc gtgagcgccg tattgaattt gctaaggata tgggagaagt
     1021 ttataatatg attcaaaaag gtagtgaaag agcgcgtgaa gttgcgggtc aaaccctatc
     1081 tgaggtaaaa ggggcaatgg gacttcatta ctttaactaa gtttatttta caagaaacta
     1141 tcatttctat ctcgaagaaa agatagtttt ttatttaccc ctgtaacatt tgacaaattt
     1201 ttatagaatg gtatgataga tacaatataa aaagagtcaa gctcaaaaag aaagaaaaga
     1261 ggaaacttcg aatgtctaat tgggacacta aatttttgaa aaaaggtttt acctttgatg
     1321 atgtattgct tattccagct gaaagtcatg tgttgcctaa cgatgcagat ttaacaacta
     1381 aattggcaga taatttgact ttaaatatcc caattattac cgctgccatg gacacagtta
     1441 cagagagtca aatggccatt gctattgctc gtgcaggtgg tctcggagtt atccataaaa
     1501 acatgtcaat tgctcaacaa gcagacgagg ttcgtaaggt aaaacgttct gaaaatggag
     1561 ttattattga tccgttcttc ttgacgcctg aacatacaat tgctgaagca gatgagctta
     1621 tgggtcgtta ccgcatcagt ggtgttccag ttgttgaaac acttgaaaat cgtaaattgg
     1681 ttggtatttt gacaaaccga gatcttcgtt ttatttcaga ttataatcaa ccaatttcaa
     1741 accatatgac tagtgaaaat cttgttactg ctcctgtggg tacggatctt gcaacggctg
     1801 agagtattct tcaagagcat cgtattgaaa aacttccgtt ggtcgatgaa gaaggcagtc
     1861 tttctggttt gatcactatc aaagatattg aaaaagttat tgagtttcca aatgcggcta
     1921 aagatgaatt tggtcgtctt ctagttgcag gtgcagtagg tgttacttca gatacatttg
     1981 aacgtgcaga ggctcttttt gaggcaggag cggatgcgat tgttattgat actgcacatg
     2041 gtcattctgc aggtgtcttg cgtaaaattg ccgagattcg tgctcatttc ccagatcgga
     2101 ctttgattgc tggaaatatt gctactgctg aaggtgcacg tgccctttat gaagcgggtg
     2161 tagacgttgt taaggttggt attggaccag gttctatctg tactactcgt gtgattgctg
     2221 gtgttggtgt tccgcaagta acagctatct acgatgctgc agctgttgcg cgcgaatatg
     2281 gtaaaacgat tattgctgac ggtgggatca agtattctgg agatattgta aaagcacttg
     2341 ctgcaggtgg aaatgctgtt atgcttggat ctatgtttgc tggaactgat gaagctccag
     2401 gcgaaactga aatcttccaa ggacgtaaat tcaagactta ccgtggtatg ggatcaattg
     2461 ctgctatgaa gaaaggttca agcgaccgtt atttccaagg ttctgtcaat gaagcaaaca
     2521 agctcgttcc agaaggaatt gaaggtcgtg ttgcttataa aggagcggca gctgatattg
     2581 ttttccaaat gattggtggt attcgctctg gtatgggtta ctgtggtgca gctaacctta
     2641 aagaactaca cgataatgct caatttattg aaatgtctgg tgctggtttg aaagaaagcc
     2701 atcctcatga tgtgcaaatt actaatgagg caccaaatta ttctatgtaa aaaacaatga
     2761 aaagaactcc agtgaaaaca ggagttcttt tacaatgttg tcaatttcca tttacagaag
     2821 ctttaccatc ctgaatagtg aagatactta gattttctgg cagattttga agatggtcta
     2881 agcttgttgt tgtgataaag gtttggattg attgagaaat cgtttctaat aattttaact
     2941 gtctagtgtt gtcaagttca ctcatcacat cgtcaagcag taatatagga gattctgtgg
     3001 taatgctttc cattaattcg atttctgcta attttatcga gaggacgaga ctacgatgtt
     3061 gaccttggct tccgaaacta gcatccatcc catttatata aaaagaaatg tcatctcgat
     3121 gaggaccgac accagtattc tttttaaata aatctctgga tctacttttt tctaaagcaa
     3181 ttttgaaaga ttcggataag ttttgtttgt cagttatatt gacagaagat tgataggata
     3241 ttgacaactc ttcgatctga ttagagagtt caaaatgttt cttacgccca aatgattcta
     3301 gtttttttat gaaatctaag cggtgattca ttacacgaca tccataatca actagctgat
     3361 catctaacac agaaaggaat gtttcatcta ttttttgagc tgattttagg taagtgtttc
     3421 tttgctttag gatgtggtta taattggtta agtcagataa atagattggc ttaatttgcc
     3481 caagttccat atcaatgaat tttcgtcgaa tcgaaggtgc tcctttaatt agttgtaaat
     3541 cttcaggagc aaataagaca acattcatgt gtcctacata atctgaaagg cgtgcctgtt
     3601 ttaagtgatt aacttttgtt acacggcctt tttgtgttag ttcgatttct aggggaatgg
     3661 atccagtttt tttctgaacg agacctgaaa gatgaagttg ttcctcatca aaatgaatga
     3721 gatttttatc tgttcgagtt cgatgactac gcgttaaggc taaaaaatag atagcctcta
     3781 acatgtttgt ttttccttgt gcattacgtc ccaaaaagac attcaattta ggattaaagt
     3841 ctattttcgt ttctttgtaa ttacgaaaag tcttgagaga tagatgttgt agccacatga
     3901 ttatctacca gggaatcgtg gagcttgttg gcttttaggt gatgaagcag gtttcgattt
     3961 gtctttttta actcccttat tcatcttttt gacaagttta gcgatccgtt ctttttcaac
     4021 tttatcagct tggtattcat cttgttcttc agaagtaggt tgtgtcaaca agatgtcaat
     4081 gttcatgtca gggatgtcaa ctttatcgcc aatacgaagt ttttttccac gacgactctc
     4141 taattcccta ttaaagtaaa cagaatgttc agagagaaat gatttaatag ctcctccgct
     4201 atgtgtaatt ccaagttctt tgagtagtgc ttggagggta ataaattctt caaataattt
     4261 gtattccata attcacctca atctttggta ttataccata ttttcctaaa aatagtgaga
     4321 gagacagcga gaaatgtaag cgatttttat ttcaaaagtg ttacagggaa caagaaaatt
     4381 tcaggttttc gtgatataat agaagtctgt atataaggag gtaaatcatg gagttagtgc
     4441 atggaatttc aacacatttt atccaatcaa aaaagtttaa aacaaacaaa attaccgtgc
     4501 gttttaccgc tccattatcc cttgatacga ttgcaggtca catgttgagt gcaagtatgc
     4561 tagagactgc taatcagatg taccccactt ctcaagattt gaggagacac ttggccagtc
     4621 tatacggtac agatatgtca accaattgtt tcagaagagg gcaaagccac attatagaat
     4681 tgacatttac ctatgttcgt gatgagtttt taagtaggaa aaacgtgcta acctctcaga
     4741 ttttggaact tgtaaaagaa actctttttt cacccgcagt agttgataat gggtttgatc
     4801 cggccttatt tgaaattgag aaaaaacaat tgctagcaag tttagcagct gatatggatg
     4861 attcttttta ttttgcacat aaagaattgg ataaattgtt ttttcatgat gaacgtcttc
     4921 aattggaata tagtgattta cgaaatcgta ttttagctga aactccacaa agttcttatt
     4981 cttgtttcca agaattttta gccaatgatc gaatagattt ctttttccta ggtgatttta
     5041 atgaggttga aattcaaaat gtattagaat catttggctt taaaggtcga aaaggagatg
     5101 tgaaggttca gtattgtcaa ccttattcta atatccttca ggaaggtatg gttcggaaaa
     5161 atgtgggaca atccattttg gaattaggtt atcattaccg ttctaaatat ggtgatgagc
     5221 aacatttacc catgattgta atgaatggtt tacttggtgg atttgctcac tctaagctct
     5281 ttacaaatgt ccgtgaaaat gctggattag cttataccat ttcaagtgag cttgatttat
     5341 ttagtggatt cttgaggatg tatgctggta tcaatcgaga aaatcgtaac caggctcgta
     5401 aaatgatgaa taatcaactg cttgatttaa aaaaaggtta ttttacagag tttgagttaa
     5461 atcagaccaa ggaaatgatt cgttggtcgt tgttactttc tcaagataat caatcttcat
     5521 tgattgaacg tgcttatcaa aatgccttat ttggaaaatc ttcagcagac tttaaaagtt
     5581 ggattgcaaa gcttgaacaa attgacaaag atgctatttg tagagtagct aataatgtga
     5641 aactacaagc gatttacttt atggaaggaa tagaatgaca aaggttgttt ttgaagaaaa
     5701 atactatcca gctgtaaaag aaaaggttta tcgaactcgt ttggccaacg gattgacagt
     5761 tgctcttttg cctaaaaagg aatttaaaga ggtttacggg agtgtcactg tacagtttgg
     5821 ttcggtagat acgtttgtca cagaagttga cggatatgta aaacaatatc ctggaggaat
     5881 tgctcatttt cttgaacata aattatttga gagagaagat tctagtgatt tgatgtcggc
     5941 ttttacgagt ctaggtgcag atagtaatgc ctttacaagc tttacaaaaa caaactatct
     6001 tttttcagca acggattatt ttttagaaaa tttagattta cttgatgaat tggtaacatc
     6061 agcacacttt actgaagctt ccattctgac agagcaggat attattcagc aagaacgaga
     6121 aatgtaccaa gatgatccag attcgtgttt attcttttca actttagcga atttgtatcc
     6181 tggtacacct ttagcaactg atatagttgg aagtgaggag tccatttccc aaatcaatct
     6241 aactaatttg caagaaaatt ttacaaagtt ttacaaacct gtaaacatgt ctctgttttt
     6301 agttggtaat tttgatgtgg agcgagtaca ggactatttt gaaagcaaag aactgaaaga
     6361 ttcagatttt caggaagtag caagagaaaa gttgttttta cagcctgtaa agccaacaga
     6421 tagtatgaga atggaagtat cttctcccaa actagcgatt ggagttagag gtaagcgaga
     6481 agtttctgaa gcggattgct atcgacatca tattttatta aaattattgt ttgcaatgat
     6541 gtttggttgg acttcggatc gttttcaaaa atgttatgaa tcaggtaaaa ttgatgcgtc
     6601 cttatctctg gaagttgaaa taacaagtcg ctttcatttt gtcatgttga caatagatac
     6661 gaaagagcca gttgctttgt ctcatcaatt taggaaggct attcgtaatt ttacaaagga
     6721 tttagatatt acagaggaac atttagatat tatcaaaaga gagatgtttg gcgaattttt
     6781 cagtagcatg aactctcttg aatttattgc aacgcaatat gatgcttttg aaaatggtga
     6841 gacaattttt gatttgccga aaattttaca ggaaattact ttagaggatg tccttgatgc
     6901 tggacatcat ttaatagatg atggtgacat agttgatttt acaatattcc catcgtagta
     6961 acctatcata atagacacta gaaagaaggg atgacaagta tgagaaaaaa aacaattgga
     7021 gaggttttac gattagctag aatcaatcag ggattgagtt tagatgaatt gcagaaaaag
     7081 acagaaatcc agttagatat gttggaagca atggaagcag acgatttcga tcaacttcca
     7141 agtccttttt acacgcgttc tttcttgaaa aaatatgcat gggctgttga gttagatgac
     7201 caaattgttt tggatgctta tgattctggg agtatgatta cttatgagga agtagatgtt
     7261 gatgaagatg agttgacagg tcgtagacgt tcaagtaaga aaaagaagaa aaaaacatca
     7321 tttttacctt tattttattt tatccttttt gctttatcga ttttaatttt tgtgacttat
     7381 tatgtttgga actatattca aactcaacca gaggagcctt ctctttctaa ttacagtgtg
     7441 gttcaatcaa caagttcaac tagctctgtt ccccactcct caagtagtag ttcttctagt
     7501 atagaatcag ctataagtgt atcaggcgaa ggaaatcatg tagaaatcgc ttataagaca
     7561 agtaaggaaa cagttaaatt gcaattggca gtttcagatg ttacaagttg ggtcagtgtt
     7621 tcagaaagcg aacttgaggg cggtgtaacc ttatcgccaa agaagaaaag tgcagaagca
     7681 acagttgcaa ctaaaagtcc tgtaacaatt acgttaggtg ttgtaaaagg tgttgatttg
     7741 acagtagata atcagactgt tgatttatcg aaattaacag ctcagactgg acaaatcact
     7801 gtaaccttta ctaaaaatta aggaaaaacg aatgaaaaaa gaacaaattc ccaatctctt
     7861 aacaataggt cgaattctct ttatacctat ttttatcttt attttgacga taggaaattc
     7921 gatagagagt catatagttg cagctattat ctttgctgtt gccagtatta ccgactattt
     7981 agatggatat ttagctcgta aatggaatgt ggtcagtaat tttggtaaat ttgcagatcc
     8041 tatggcggat aagttactag ttatgtcggc ttttattatg ttgattgagt taggtatggc
     8101 tccggcttgg attgttgcag tgattatctg tcgtgagtta gctgtgacag gtttaaggct
     8161 tttattggtt gaaactggtg gaacaatttt agcagcagca atgcctggaa aaattaaaac
     8221 ttttagtcag atgtttgcta ttattttctt gctattacat tggactttgc ttggtcaagt
     8281 tctactttat gtagccttat ttttcactat ctactctggc tatgactatt tcaagggtag
     8341 tgcctatgta tttaaaggga catttggttc gaaatgaaat caataattga tgtaaaaaat
     8401 ctttcttttc gctataaaga aaatcagaac tactacgatg tgaaggatat tacgtttcac
     8461 gtgaaacgtg gagaatggct ttcgattgta gggcataatg gtagtggtaa atcaacgacg
     8521 gttcgattaa ttgatggctt actggaagca gaatccggag agattgtaat tgatggccaa
     8581 cgtttgactg aggaaaatgt ttggaatata cgtcgtcaaa tcggtatggt ttttcaaaat
     8641 ccagacaatc aatttgttgg agcgactgtt gaagatgatg ttgcttttgg tttggaaaat
     8701 cagggacttt ctcgtcaaga aatgaaaaag agagtggaag aagctctggc tttagttggc
     8761 atgttggact ttaaaaagag agagccagcg cgtctatcag gtggccaaaa gcaacgtgtg
     8821 gccattgcag gtgttgtagc cctaagacca gctattttaa tcttagatga agcaacgagt
     8881 atgttggatc ctgaggggcg tagagaactt attgggacag taaaaggaat tcgaaaagac
     8941 tatgatatga cggtcatttc tattacccat gatttggaag aagtcgccat gagtgatcgc
     9001 gtattggtca tgaaaaaagg ggaaattgaa tcaactagta gtccaaggga gcttttctct
     9061 cgaaatgatt tagatcaaat tggattagac gatccttttg ctaatcaatt aaaaaaatct
     9121 ttgagccaga atggctatga tttacctgaa aattatttga cagaaagtga gctagaggat
     9181 aagctatggg aattgctcta gaaaatgtga attttatata tcaagaaggt actcccttag
     9241 cttcagcagc tttgtcggat gtttctttga cgattgaaga tggctcttat acagctttaa
     9301 ttgggcacac aggtagtggt aaatcaacta ttttacaact cttaaatggt ttattggtgc
     9361 caagtcaagg gagtgtgagg gtttttgata ccttaatcac ctcgacttct aaaaataaag
     9421 atattcgtca aattagaaaa caggttggct tggtatttca gtttgctgaa aatcagattt
     9481 ttgaagaaac ggttttgaag gacgttgctt ttggaccgca aaattttgga gtttctgaag
     9541 aagatgctgt gaagactgcg cgtgagaaac tggctctggt tggaattgat gaatcacttt
     9601 ttgatcgtag tccgtttgag ctgtcagggg gacaaatgag acgtgttgcc attgcaggca
     9661 tacttgccat ggagccatct atattagtct tagatgagcc aacagctggt ctagatcctc
     9721 tagggagaaa agagttgatg accctgttca aaaaactcca ccagtcaggg atgaccatcg
     9781 tcttggtaac gcatttgatg gatgatgttg ctgaatatgc taatcaagtc tatgtaatgg
     9841 aaaagggacg tttagtaaag gggggcaaac caagtgatgt ctttcaagac gttgttttta
     9901 tggaagaagt tcagttggga gtacctaaaa ttacggcctt ttgtaaacga ttggctgata
     9961 gaggcgtgtc atttaaacga ttaccgatta agatagagga gttcaaggag tcgctaaatg
    10021 gatagtatga ttttggggcg ttatatccca ggggattcga ttgttcaccg attggatcca
    10081 cgtagcaaat tgcttgctat gatgctactg attttgattg tgttttgggc taataacccc
    10141 ttgacgaatc tgattctttt tatagcgaca gggatattta ttgccttgtc aggagtatct
    10201 ctttcatttt ttattcaggg cttgaagtct atgtttttct tgattgcctt cacaactatt
    10261 tttcaactat ttttcatttc taatgggaat gttttatttg agttttcgtt tgtgagaatc
    10321 acggattatg ctttgcaaca agctgggatt attttttgtc gctttgtatt gattattttc
    10381 ttttcaactt tgttaacctt aacaaccatg cctttaagtt tggcatcagc tgtcgaagct
    10441 ttattagcac ctttaaagcg tgtgaaagtt ccagttcatg aaattggatt gatgctgtct
    10501 atgagtttgc gttttgtccc aaccttgatg gatgatacga ctcggattat gaatgcacag
    10561 aaagctcgtg gagtggattt tggagaagga agcatcgttc aaaaagtaaa ggcgatgatt
    10621 cccattttga ttcctctttt tgcgacaagt ttaaaacgtg cagattcctt ggctatcgct
    10681 atggaagcgc gtggctatca gggtggaaaa ggcagaagtc aataccgaca attgaaatgg
    10741 actctaaagg atacgctgac cattcttgtt attctcgtac ttggttgttg tttatttttc
    10801 ttaaaatctt agtagctttc aggaattgat aaaaagttac tgtaacagtt tttgatataa
    10861 aggtagggat atgaaccgtt ttaaaaaatc aaaatatgtc attattgttt ttgtcactgt
    10921 tctgcttgtg tcagctctct tagcgacgac ttattcaagt acaattgtga caaaattagg
    10981 agatggaatc tcattggttg atagagttgt acaaaaacct tttcagtggt ttgattctgt
    11041 caaatcagat ttggctcatt tgacacgaac atataatgaa aatgaaagtt tgaagaaaca
    11101 gctttaccaa ttagaagtta aatcaaatga ggtggaaagt ttaaagacag aaaatgaaca
    11161 actgcgccaa ttgcttgata tgaagtctaa attgcaagcc acaaagactt tagcagcaga
    11221 tgttattatg cgttctccgg tatcttggaa gcaggagttg accttagatg caggtagatc
    11281 aaaaggtgct tctgagaaca tgttagctat tgcaaatggt ggcttgattg ggagtgtttc
    11341 aaaagtagag gagaactcta ctatagtcaa ccttctgaca aatacggaaa atgctgataa
    11401 gatttctgtt aaaattcaac atggctctac tacaatttat ggaattatta ttggctatga
    11461 caaggaaaat gacgttctta aaattagcca attaaatagt aatagcgata ttagtgcggg
    11521 agataaggtg actactggtg gattaggaaa ctttaacgtt gctgatattc ctgttggtga
    11581 agtggttgcc acaacgcata gtacagacta tttgacacga gaagtaactg ttaaattgag
    11641 tgcagatact cataatgtag atgtgataga attagtgggg aattcataat gagacagttg
    11701 aagcgagttg gagtattttt attgcttcct ttctttgttc taattgacgc ccatattagc
    11761 cagcttctgg gctcattttt cccccatgta catttggcta gtcattttct ttttctattt
    11821 ctcttatttg agacgataga agtatcagag tatctctacc tagtctattg ttttgttata
    11881 ggcttggttt atgatgttta ctttttccat ctaataggga ttacaactct cttatttatc
    11941 ttattgggag ccttccttca taaattgaat agtgttattt tgttgaatcg ttggacaaga
    12001 atgctagcta tgattgtgct gacattcctg tttgaaatgg gtagttatct tttggctttt
    12061 atggtagggt tgacagtaga tagcatgtcg atttttatag tctatagctt ggtaccgacg
    12121 atgattttaa attttttatg gattactgtt tttcaattta tttttgaaaa atattatcta
    12181 taagaacgac atataaatgt aacaaaggcg taatatttat taggcctttt tttggtatac
    12241 tagtattgtc tttaaaagaa ggagtatcta cgtaatatga agaaaaaaat cttagcgtca
    12301 cttttattaa gtacagtaat ggtttctcaa gtagctgttt taacaactgc gcatgcagaa
    12361 acgactgatg acaaaattgc tgctcaagat aataaaatta gtaacttaac agcacaacaa
    12421 caagaagccc aaaaacaagt tgaccaaatt caggagcaag tatcagctat tcaagctgag
    12481 cagtctaact tgcaagctga aaatgataga ttacaagcag aatctaagaa actcgagggt
    12541 gagattacag aactttctaa aaacattgtt tctcgtaacc aatcgttgga aaaacaagct
    12601 cgtagtgctc aaacaaatgg agccgtaact agctatatca ataccattgt aaactcaaaa
    12661 tcaattacag aagctatttc acgtgttgct gcaatgagtg aaatcgtatc tgcaaacaac
    12721 aaaatgttag aacaacaaaa ggcagataaa aaagctattt ctgaaaaaca agtagcaaat
    12781 aatgatgcta tcaatactgt aattgctaat caacaaaaat tggctgatga tgctcaagca
    12841 ttgactacga aacaggcaga actaaaagct gctgaattaa gtcttgctgc tgagaaagcg
    12901 acagctgaag gggaaaaagc aagtctatta gagcaaaaag cagcagctga ggcagaggct
    12961 cgtgcagctg cggtagcaga agcagcttat aaagaaaaac gagctagcca acaacaatca
    13021 gtacttgctt cagcaaacac taacttaaca gctcaagtgc aagcagtatc tgaatctgca
    13081 gcagcacctg tccgtgcaaa agttcgtcca acatacagta caaacgcttc aagttatcca
    13141 attggagaat gtacatgggg agtaaaaaca ttggcacctt gggctggaga ctactggggt
    13201 aatggagcac agtgggctac aagtgcagca gcagcaggtt tccgtacagg ttcaacacct
    13261 caagttggag caattgcatg ttggaatgat ggtggatatg gtcacgtagc ggttgttaca
    13321 gctgttgaat caacaacacg tatccaagta tcagaatcaa attatgcagg taatcgtaca
    13381 attggaaatc accgtggatg gttcaatcca acaacaactt ctgaaggttt tgttacatat
    13441 atttatgcag attaatttac agagggactc gaatagagcc ctcttttcag gttttaccgt
    13501 gacaatccct attaaaaatt atatcaaaat agcttgaaaa tattggaaaa gtatggtaaa
    13561 atgaaaattg tcgtgtgaac gataatactc attcttgatg aattgtgaag cagttgccct
    13621 tgggtcgttt tgcgagttga agtcaagaag aggaaaaaaa caaaaaggag aaatactcat
    13681 ggcagtaatt tcaatgaaac aacttcttga ggctggtgta cactttggtc accaaactcg
    13741 tcgctggaat cctaagatgg ctaagtacat ctttactgaa cgtaacggaa tccacgttat
    13801 cgacttgcaa caaactgtaa aatacgctga ccaagcatac gacttcatgc gtgatgcagc
    13861 agctaacgat gcagttgtat tgttcgttgg tactaagaaa caagcagctg atgcagttgc
    13921 tgaagaagca gtacgttcag gtcaatactt catcaaccac cgttggttgg gtggaactct
    13981 tacaaactgg ggaacaatcc aaaaacgtat cgctcgtttg aaagaaatta aacgtatgga
    14041 agaagatgga actttcgaag ttcttcctaa gaaagaagtt gcacttctta acaaacaacg
    14101 tgcgcgtctt gaaaaattct tgggtggtat cgaagatatg cctcgtatcc cagatgtgat
    14161 gtacgtagtt gacccacata aagagcaaat cgctgttaaa gaagctaaaa aattgggaat
    14221 cccagttgta gcgatggttg acaccaatac tgatccagat gatatcgatg taatcatccc
    14281 agctaacgat gacgctatcc gtgctgttaa attgatcaca gctaaattgg ctgacgctat
    14341 tatcgaagga cgtcaaggtg aggatgcagt agcagttgaa gcagaatttg cagcttcaga
    14401 aactcaagca gattcaattg aagaaatcgt tgaagttgta gaaggtgaca acaaatagta
    14461 attacctagg agggcggggc ttagcccggc tctcctattt tcaaaaaata taggagaatt
    14521 aaaatggcag aaattacagc taaacttgta aaagagttgc gtgaaaaatc tggtgccggt
    14581 gttatggacg ctaaaaaagc gcttgtagaa acagacggtg acatcgaaaa agcgattgaa
    14641 ttgcttcgtg aaaaaggtat ggctaaggca gctaagaaag ctgaccgtgt tgctgcagaa
    14701 ggtttgactg gtgtttatgt taacggtaat gttgcagcag ttattgaagt aaacgctgaa
    14761 actgacttcg ttgcaaaaaa cgctcaattc gttgaattgg taaatactac agctaaagtc
    14821 attgctgaag gaaaacctgc taataatgaa gaagctcttg ctttgataat gccttcaggt
    14881 gaaactcttg aagctgcata cgtatctgca acagcaacta tcggagagaa aatctcattc
    14941 cgtcgctttg cattgattga aaaaacagac gcacaacact ttggagcata ccaacataac
    15001 ggtggacgta tcggtgttat ttcagttgtt gaaggtggag acgaagcact tgctaaacaa
    15061 ttgtcaatgc acatcgcagc gatgaaacca acagttcttt cttacaaaga attggatgag
    15121 caattcgtta aagatgagtt ggcacaattg aatcacgtta tcgaccaaga caacgaaagc
    15181 cgtgcaatgg ttaataaacc agctcttcca cacttgaagt atggatcaaa agctcaatta
    15241 actgatgatg ttattgctca agctgaagct gacatcaaag ctgaattggc tgcagaaggc
    15301 aaaccagaaa aaatctggga caaaattatc ccaggtaaaa tggatcgctt catgcttgat
    15361 aatactaaag ttgaccaagc ttacacactt cttgcacaag tttacatcat ggatgacagc
    15421 aagacagttg aagcatacct tgaatcagtt aacgcttcgg tagttgagtt tgctcgcttt
    15481 gaagttggtg aaggtatcga gaaagctgca aacgacttcg aagctgaagt tgcagctaca
    15541 atggcagcag ccttgaataa ctaataatag aaaggaagca aattgcttcc ttttttgtat
    15601 agggcttaga tttttcctga aattgttttt tccccagtct cactcgatta gaataattaa
    15661 aacaaaaagc cctataataa gggctaagtg catttaggag actatacttc aaattcatag
    15721 taagcgcgtc ataacaaggt atctatccta attccccaac ttccagtata ccagataaaa
    15781 aataattatc ggaggaaagg tattgtacta tgataattca actaagtgat ttaggtcaag
    15841 ttcaccttgt ttgtggcaag acagatatga ggcagggaat agactcatta gcctatgtag
    15901 ttaaaaccca ctttgaattg gatcctttct ccgatcaagt ctttctcttt tgtggtggac
    15961 gtaaagaccg ctttaaagtt ctttactggg atggtcaagg attttggata ttatataaac
    16021 gctttgagaa cggaaaactg acttggtcca gtacagaaaa ggatgtcaaa gctctcacat
    16081 ctgaacaagt agattggctg atgaagggct tttctatcac tccccaaata tagtggattt
    16141 aaactagaat agtacacctc tgcttctaaa acattgttag aaatcgattt gactatcctg
    16201 ttcttatttc attttactat aaattcatca gaaagtcgtg atttctattg aaatgaggac
    16261 tttcttttta tactcatctg ttttcaaaaa gcattctagt ccgtctccga ttaacgatgg
    16321 actttatcac ctccttctcc agtccttgta tgacatcttc caaagttcga aaggctttat
    16381 tcttaaatcc acgtttacga atctctttcc acacttgttc aatggggttc atctctggtg
    16441 tgtatggagg aataaatgca aagccaatat tagtcggaat ctttaaggta cttgatttat
    16501 gccatatagc attgtccata acgagtaaaa gataatcatc tggataagct tgtgaaagct
    16561 cctattccta aagccccttt agcgcataac tttggctcag cttctattat cgctcacacc
    16621 atccatcaga agtttaatct gaaggtaccc aattatcgcc aagaagaaga ttgggctagg
    16681 atgggtttac caatcacacg taaggaaatc tctaattggc atatcaagac gagtcaatac
    16741 tatttggagc ccctttataa cctcttgcga gagagactat tgactcagcc cttacttcat
    16801 gcggatgaaa cttcttatag ggtgctagag agtgatagtc agctgactta ctattggact
    16861 tttttgtctg ggaaagctga gaaacaaggg attacgcttt accatcatgt tctaatcgat
    16921 ttgttcatat cttatttcaa tccattataa atagtgagaa atatctatcc tatcttctag
    16981 aatgtcttcc aaacgtggaa actctcgtaa acaaagaggt tttagaggcc tatttaccat
    17041 ggactaaagt tgtacaagaa aagtgcaaat aagaaatctc cagattagga actatccgtg
    17101 agttctctag tctggagatt tttcaataga cttcgttatt ggacggttac aattcataaa
    17161 gtgctgtaga gagataacgt tcaccgttat ctggtgctag ggcaaggact tttttacctg
    17221 tacctaattt tttggcaacc tcgatggctc cgtagatagc tgcagctgag gaaatcccta
    17281 caaggaagcc ttcttttcca ccaatttcac gtccgagtgc aagagcgtca tctgatgtta
    17341 cacgaacgat accatcatag gctttagtat caagtgtatc aggaataaat ccagctgaga
    17401 taccttgaat tttgtgagga ccaggttttt caccagatag aatagcagat tcatctgctt
    17461 ctactgcaaa aacttgaatg ttagaatttt ctgatttgag tgcatgagaa acaccagaaa
    17521 tcgttccacc agtacctact ccagcaacaa aggcatctaa tccatcttta ccgaaagcag
    17581 ctagtatctc agctcctgtt gttctttcgt gtacttctgg attagctgga ttgtcaaatt
    17641 gaagaggaag gaaaccatca cgttcagcag cgatttcttg agccttagca atagcacctt
    17701 tcattccctc gctaccagga gttaggacga gttcagcacc ataagcttgg ataattttac
    17761 gtcgttctac actcatagtt tcaggcataa cgatgacgac tttataccct ttagcagcac
    17821 ctacccatga aagtccaata ccggtgtttc cacttgttgc ttcaacaata gtagaaccag
    17881 gtttcagaat accatcttgt tcagcttttt caatcatgct aagggcaata cggtctttta
    17941 cagatgaacc aggattaaat gcttcaagct ttatatagac gtctgcagca ccttctggca
    18001 cgatgttgtt aagtttaaca atcggtgttt gaccgattaa ttcagtaatg ttgttataaa
    18061 tagacatatg aatacctctt tgtataagat atctttagta taacgctatc cataccattt
    18121 gtaaaatata gaaatcctat cgaagcgata ggatttctat atatcataga tcaaagtcat
    18181 taattttcaa gtgattgcgt tcagctaatt ctagtaaata ggtgtgaagg aaaacgatat
    18241 caggtttcgt ttgaattgct tcattttcta gtatattttg gtacgcatct ttttattttg
    18301 ctcgctaaag tagttgtgag aatgtcaacg aaacgctgta ttattcattg tttacactaa
    18361 gtttacagga acttcaacct ctcgtaaacc ttggtcagtt aaagtgactt ttccattaaa
    18421 aaactccaca agtgcagctt taatagtttc tttttcttct ttatcaacat aaatcatcgt
    18481 atcgacttga tctgtaaagt ttgtatccag ctccatgaga ccatgttctt taaggaagtt
    18541 actgtactct tggtactgag cataagacat ttgaatagca atgccagcct gttcttttat
    18601 ttcaataata ccaatttctt tgacagctaa ggcgacactg ccggcgtaag cacgaattag
    18661 tcctccagcg cctagtttaa taccaccaaa gtagcgtgtc acgaccacac agacattggt
    18721 gagattgtga ttttctagta ccccaagcat gggaacacca gcagtaccac taggctcacc
    18781 atcatcactt gtacgtttaa tttcactacg ttctccaata atgaaggcag agcagttatg
    18841 tgtcgctttg tagtgttctt ttttgatggc agtaatgaag tcacgagcct cttcttcgct
    18901 ataaacacgc ttggcatggc agataaagcg agattttttg atttcttctt ggacttgacc
    18961 gtcctcttta attgtcctaa attccatgat ttaattgtac taaaaaatca tctaaagtgc
    19021 tagtttttac gaataaagaa gtatgaaagt aaatttagat tatctcggtc gtttatttac
    19081 tgagaatgaa ttaacagaag aagaacgtca gttggcggag aaacttccag caatgagaaa
    19141 ggagaagggg aaacttttct gtcaacgctg taatagtact attctagaag aatggtattt
    19201 gcccatcggt gcttactatt gtcgagagtg cttgctgatg aagcgagtca gaagtgatca
    19261 aactttatac tattttccgc aggaggattt tccaaagcaa gatgttctca aatggcgcgg
    19321 ccaattaact ccttttcaag agaaggtgtc agagggattg cttcaagtag tagacaagca
    19381 aaagccaacc ttagttcatg cggtaacagg agctggaaag acagaaatga tttatcaagt
    19441 agtggctaaa gtgatcaatg cgggtggtgc agtgtgtttg gctagtcctc gcatagatgt
    19501 ttgtttggag ctgtacaagc gcctgcaaca ggatttttct tgcgggatag ctttgctaca
    19561 tggagaatcg gaaccttatt ttcgaacacc actagttgtt gcaacaaccc atcagttatt
    19621 gaagttttat caagcttttg atttgctgat agtggatgaa gtagatgctt ttccttatgt
    19681 tgataatccc atgctttacc acgctgtcaa gaatagtgta aaggagaatg gattgagaat
    19741 ctttttaaca gcgacttcga ccaatgagtt agataaaaag gtccgtttag gagaactaaa
    19801 aagactgaat ttaccgagac ggtttcatgg aaatccgttg attattccaa aaccaatttg
    19861 gttatcggat tttaatcgct acttagacaa gaatcgtttg tcaccaaagt taaagtccta
    19921 tattgagaag cagagaaaga cagcttatcc gttactcatt tttgcttcag aaattaagaa
    19981 aggggagcag ttagcagaaa tcttacagga gcaatttcca aatgagaaaa ttggctttgt
    20041 atcttctgta acagaggatc gattagagca agtacaagct tttcgagatg gagaactgac
    20101 aatacttatc agtacgacaa tcttggagcg cggagttacc ttcccttgtg tggatgtttt
    20161 cgtagtagag gccaatcatc gtttgtttac caagtctagt ttgattcaga ttggtggacg
    20221 agttggacga agcatggata gaccgacagg agatttgctt ttcttccatg atgggttaaa
    20281 tgcttcaatc aagaaggcga ttaaggaaat tcagatgatg aataaggagg ctggtctatg
    20341 aagtgcttgt tatgtgggca gactatgaag actgttttaa cttttagtag tctcttactt
    20401 ctgaggaatg atgactcttg tctttgttca gactgtgatt ctacttttga aagaattggg
    20461 gaagagaact gtccaaattg tatgaaaaca gagttgtcaa caaagtgtca agattgtcaa
    20521 ctttggtgta aagaaggagt tgaagtcagt catagagcga tttttactta caatcaagct
    20581 atgaaggatt ttttcagtcg ctataagttt gatggagact tcctgttaag aaaagttttc
    20641 gcttcatttt taagtgagga gttgaaaaag tacaaagagt atcaatttgt tgtaattccc
    20701 ctaagtcctg atagatatgc taatagagga tttaatcagg ttgagggctt ggtagaggca
    20761 gcaggctttg agtatctgga tttattagag aaaagagaag agagagccag ttcttctaaa
    20821 aatcgttcag agcgcttggg gacagaactt cctttcttta ttaaaagtgg agtcactatt
    20881 cctaaaaaaa tcctacttat agatgatatc tatactacag gagcaactat aaatcgtgtt
    20941 aagaaactgt tggaagaagc tggtgctaag gatgtaaaaa cattttccct tgtaagatga
    21001 ggaaaaaatt tgcaaaaata gaatgaaagc gttataataa aatcataaaa acaaaaatgt
    21061 ttagaaagaa ggtactcata tgattaaata tagtatccgt ggtgaaaacc tagaagtaac
    21121 agaagcaatt cgtgattatg tagtttctaa actcgaaaag atcgaaaagt acttccaacc
    21181 agaacaagag ttggatgccc gaattaactt aaaagtttat cgtgaaaaaa cggctaaagt
    21241 ggaagtaacg attccgcttg gatctattac tctccgcgca gaagatgtat ctcaagatat
    21301 gtatggttca attgaccttg taactgataa aattgaacgt cagattcgta aaaataaaac
    21361 aaaaatcgag cgtaaaaata aaaataaggt agcaactggt caattattta cagatgcttt
    21421 ggtggaagat tcaaatgttg tccagtctaa agttgttcgt tcaaaacaaa ttgatttaaa
    21481 accaatggat ttggaagaag caattctaca aatggattta ttggggcatg atttctttat
    21541 ctatgtggat gttgaagatc agacaaccaa tgtgatttat cgtcgtgagg atggcgaaat
    21601 tggtttgtta gaggttaaag aatcttaatt tcaatatgtg taaaggtagg tttactgaat
    21661 tgtaaactac ctttttctta taattgatag aattactgat tacactttta aaaagtcgct
    21721 ttttgagggg tattatggta taatgggtgc caagaggttt tgaatgaaaa aattttatgt
    21781 aagtccaatt tttcctattc tagtaggatt gattgcgttt ggagtcttat ccactttcat
    21841 tatttttgtt aataataatc tgttgacggt tttaattttg tttctttttg taggaggcta
    21901 tgttttttta tttaagaaac tgagagtgca ttatacaagg agtgatgtag aacagataca
    21961 gtatgtaaac caccaagcgg aagaaagttt gacatctcta ttggaacaaa tgcctgtagg
    22021 tgttatgaaa ttgaatttat cttctggaga ggttgagtgg tttaatccct atgctgaatt
    22081 gattttgacc aaggaagatg gtgattttga tttagaagct gttcaaacga ttatcaaggc
    22141 ttcagtagga aatccgtcta cttatgccaa gcttggtgag aagcgttatg ctgttcatat
    22201 ggatgcttct tccggtgttt tgtattttgt agatgtatcc agggaacaag ccataacaga
    22261 tgaattggta acaagtcgac cagtgattgg gattgtctct gtggataatt atgatgattt
    22321 ggaggatgca acttctgagt cagatattag tcaaatcaat agttttgtag ctaattttat
    22381 atcagagttt tcagaaaaat acatgatgtt ttctcgtcgg gtaagtatgg atcgatttta
    22441 tctatttact gactacacgg tgcttgaggg cttgatgaat gataaatttt ctgttattga
    22501 tgctttcaga gaagagtcga aacagagaca gttgcccttg accttaagta tgggattttc
    22561 ttatggcgat ggaaatcatg atgagatagg gaaagttgct ttgctcaatt tgaacttggc
    22621 tgaagtacgt ggtggcgacc aggtggttgt taaggaaaac gacgaaacga aaaatccagt
    22681 ttattttggt ggtgggtctg ctgcttcaat caagcgtaca cggactcgta cgcgcgctat
    22741 gatgacagct atttcagata agattcggag tgtagatcag gtttttgtag tcggtcacaa
    22801 aaatttagac atggatgctt tgggctctgc tgtaggtatg cagttgttcg ccagcaatgt
    22861 gattgaaaat agctatgctc tttatgatga agaacaaatg tctccagata ttgaacgagc
    22921 tgtttcattc atagaaaaag aaggagttac gaagttgttg tctgttaagg atgcaatggg
    22981 gatggtgacc aatcgttctt tgttgattct tgtagaccat tcaaagacag ccttaacatt
    23041 atcaaaagaa ttttatgatt tatttaccca aaccattgtt attgaccacc atagaaggga
    23101 tcaggatttt ccagataatg cggttattac ttatatcgaa agtggtgcaa gtagtgccag
    23161 tgagttggta acggaattga ttcagttcca gaattctaag aaaaatcgtt tgagtcgtat
    23221 gcaagcaagt gtcttgatgg ctggtatgat gttggatact aaaaatttca cctcgcgagt
    23281 aactagtcgg acatttgatg ttgctagcta tctcagaacg cgcggaagtg atagtattgc
    23341 tatccaggaa atcgctgcga cagattttga agaatatcgt gaggtcaatg aacttatttt
    23401 acaggggcgt aaattaggtt cagatgtact aatagcagag gctaaggaca cgaaatgcta
    23461 tgatacagtt gttattagta aggcagcaga tgccatgtta gccatgtcag gtattgaagc
    23521 gagttttgtt cttgcgaaga atacacaagg atttatctct atctcagctc gaagtcgtag
    23581 taaactgaat gtacaacgga ttatggaaga gttaggcggt ggaggccact ttaatttggc
    23641 agcagctcaa attaaagatg taaccttgtc agaagcaggt gaaaaactga cagaaattgt
    23701 attaaatgaa ataaaggaaa aggagaaaga agaatgaaag taatcttttt agcagatgtt
    23761 aaaggaaaag gtaaaaaagg cgaaattaag gaagtaccaa cagggtatgc gcaaaacttt
    23821 cttatcaaaa agaatctagc caaagaagcg actgctcaag ctgtaggtga acttcgtggt
    23881 aaacaaaaat cggaagaaaa agctcacgct gagatgattg cagaaggaaa agcaattaaa
    23941 gcacaacttg aagcagaaga aactgttgta gaatttgttg aaaaagttgg tccagatggt
    24001 cgtacctttg gttctattac caataagaag attgcagaag aattgcaaaa gcaatttgga
    24061 attaagattg ataaacgtca tattcaagta caagctccga ttcgagcggt tggtttgatt
    24121 gatgtgccag tgaaaatcta tcaagatatc acaagtgtaa tcaatcttcg tgtgaaagaa
    24181 ggataagttt acaccttctt gacaagattg taaaaggaag ggaagtctga tggcagaagt
    24241 agaagagtta cgagtacaac ctcaagatat cttagctgag caatccgttt taggggctat
    24301 ctttattgat gagagtaaac ttgtttttgt gcgagaatac attgagtctc gggacttttt
    24361 taagtatgcc catcgtttga ttttccaagc catggtcgat ttatccgatc gtggtgatgc
    24421 catagatgca acaacggttc gtactatcct tgataatcaa ggtgatttac agaatattgg
    24481 tggcttgtct tacttggttg agattgttaa ttctgtgcca acttctgcta atgcggagta
    24541 ttatgctaag attgttgcag aaaaagcaat gctacgtcgt ttaattgcca agttgacaga
    24601 gtctgtcaac caagcttacg aagcgtcaca accagctgat gaaattattg ctcaggcaga
    24661 aaaagggttg attgatgtca gtgaaaatgc aaatcgaagc gggtttaaga acattcgaga
    24721 tgtgttgaat ctcaactttg gaaatctgga agctcgctcg caacaaacga ccgatattac
    24781 aggtattgcg acaggttatc gtgatttgga tcatatgaca acaggacttc atgaggagga
    24841 gttgattatc ttagcagctc gtccagcagt tggtaagaca gcatttgcct tgaatatcgc
    24901 tcagaatatt gggactaagt tggacaaaac ggttgctatt ttttcactcg aaatgggtgc
    24961 ggaaagcttg gtagaccgta tgttagctgc agaaggcttg gtggagtcac attctatccg
    25021 tacagggcaa ttgacagatg aggagtggca aaaatatact attgctcagg gtaatctagc
    25081 taacgccagt atctatatcg atgatacgcc aggtattcgg attacagaga ttcgttctcg
    25141 ttctcgtaaa ttggctcaag aaactggaaa tcttggtttg attttgatag actatttgca
    25201 acttatcacg ggaactggtc gagaaaatcg tcaacaagaa gtttctgaaa tttctcgtca
    25261 gttgaaaata ctagccaagg aattgaaggt tccagtaatc gctctgagtc agctttctcg
    25321 tggtgtagaa caacgtcagg acaagagacc ggtcttgtct gatattcgtg aatctgggtc
    25381 tattgagcag gacgctgata tcgtagcttt tctctatcgc gatgactact atgaacgtgg
    25441 tggtgaagaa gaggagggta tcccaaataa taaggtggaa gttattatcg agaaaaaccg
    25501 tagtggagct cgtggaacag tggaattgat tttccaaaaa gaatacaata aattttcaag
    25561 tatctcaaag agggaggcat aagatgtcag atgcatttac agatgtagcc aagatgaaaa
    25621 aaatcaaaga agaaatcaag gcacatgagg gacaagtcgt agaaatgact ttggagaatg
    25681 gtcgtaagcg ccaaaaaaat agattgggta aactaattga agtttatcca tccctattta
    25741 ttgtggagtt tggggatgtg gaaggagata aacaagttaa tgtttacgtt gaatccttta
    25801 cttactcaga tattcttaca gaaaagaatt tgattcatta tcttgactaa agtgagaaat
    25861 tttctcactt tttctttttt ctccgaataa tttaggtgaa ggcaatcatc gctttatatt
    25921 attttttaag gaggaagaat gaaaatttta ccgtttatag caagaggaac aagttattac
    25981 ttgaagatgt cagttaaaaa gcttgttcct tttttagtag taggattgat gctagcagct
    26041 ggtgatagtg tctatgccta ttccagagga aatggatcga ttgcgcgtgg ggatgattat
    26101 cctgcttatt ataaaaatgg gagccaggag attgatcagt ggcgcatgta ttctcgtcag
    26161 tgtacttctt ttgtagcctt tcgtttgagt aatgtcaatg gttttgaaat tccggcagct
    26221 tatggaaatg cgaatgaatg gggacatcgt gctcgtcggg aaggttatcg tgtagataat
    26281 acaccgacga ttggttccat tacttggtct actgcaggaa cttatggtca tgttgcctgg
    26341 gtgtcaaatg taatgggaga tcagattgag attgaggaat ataactatgg ttatacagaa
    26401 tcctataata aacgaattat aaaagcaaac acgatgacag gatttattca ttttaaagat
    26461 ttggatggtg gcagtgttgg gaatagtcaa tcctcaactt caacaggcgg aactcattat
    26521 tttaagacca agtctgctat taaaactgaa cccctagtta gtgcaactgt gattgattac
    26581 tattatcctg gagagaaggt tcattatgat caaattctcg aaaaagacgg ctacaagtgg
    26641 ttgagttata ctgcctacaa tggaagctat cgttatgttc aattggaggc tgtgaataaa
    26701 aatcctctag gtaattctgt tctttcttca acaggtggaa ctcattattt taagaccaag
    26761 tctgctatca aaactgaacc cctagttagt gcaactgtga ttgattacta ttatcctggg
    26821 gagaaggttc attatgatca gatacttgaa aaagacggct acaagtggtt gagttatacg
    26881 gcttataacg gaagtcgtcg ctatatacag ctagagggag tgacttcttc acaaaattat
    26941 cagaatcaat caggaaatat ctctagctat ggatccaata atagttcaac tgtcggttgg
    27001 aagaaaataa atggtagttg gtatcatttc aaatcaaatg gttctaaatc aacaggatgg
    27061 ctgaaagatg gttctagctg gtattatttg aaattatctg gtgaaatgca gacaggatgg
    27121 ttaaaggaga atggctcgtg gtattatctg ggtagttcag gggcaatgaa aacaggctgg
    27181 taccaggtct ctggtaagtg gtattattct tactcttcag gcgccttagc tgttaatacg
    27241 acggtggatg gctacagagt aaacagtgat ggagaacgag tatagaaaat tggagtagga
    27301 gaaatttcct gctcttttct gtaagcagtt tccttgacat ttttcttatt ttgcgctatc
    27361 atatatacat ataatatatg ggagtctgtg tacagtctga gaggaagtgt taaacttcga
    27421 ccgcacctga tctgggtaat gccagcggag ggaacgatac ttagtctaat tttgcacctt
    27481 ttccatgtat ggtaaaggtt tttctttttt taaaaaggaa aacgagaaga ggaggttctt
    27541 atgaaagcaa gcattgcctt gcaagtttta cccctagcac aggggattga tcggatagct
    27601 gttattgatc aggtcattgc ttatctgcaa actcaagaag tgacgatggt agtgacacca
    27661 tttgaaacgg tcttggaagg ggagtttgat gagcttatgc gcattctaaa agaagcgctg
    27721 gaagtggcag ggcagggggc agacaatgtc ttttccaatg tcaaaataaa tgtaggagag
    27781 attttaagta ttgatgagaa acttgagaag tatactgaga cgacacatta gtctattggg
    27841 ctttctcgga gtcttgtcaa tctggcagtt agcaggtttt cttaaacttc tccccaagtt
    27901 tatcctgccg acacctcttg aaattctcca gccctttgtt cgtgacagag aatttctctg
    27961 gcaccatagc tgggcgacct tgagagtggc tttactgggg ctgattttgg gagttttgat
    28021 tgcctgtctt atggctgtac tcatggatag tttgacttgg ttcaatgacc tgatttaccc
    28081 tatgatggtg gtcattcaga ccattccgac cattgccata gctcctatcc tggtcttgtg
    28141 gctaggttat gggattttgc ccaagattgt cttgattatc ttaacaacaa cctttcccat
    28201 catcgttagt attttggacg gttttaggca ttgtgacaag gatatgctga ccttgtttag
    28261 tctgatgcga gcaaaccctt ggcaaatcct gtggcatttt aaaatcccgg ttagcctgcc
    28321 ttacttttat gcaggtctga gggtcagtgt ctcctacgcc tttatcacaa ctgtggtatc
    28381 tgagtggttg ggaggttttg aggggcttgg tgtttatatg attcagtcca agaaattgtt
    28441 tcagtatgat accatgtttg ccattattat tctggtgtcg attatcagtc ttttgggtat
    28501 gaagctggtc gatatcagtg aaaaatatgt gattaaatgg aaacgtactt aaaatagagc
    28561 gtttttgaaa aagaaaagag gaaatcaaaa tgaagaaaac atggaaagtg tttttaacgc
    28621 ttgtaacagc tcttgtagct gttgtgcttg tggcctgtgg tcaaggaact gcttctaaag
    28681 acaacaaaga ggcagaactt aagaaggttg actttatcct agactggaca ccaaatacca
    28741 accacacagg gctttatgtt gccaaggaaa aaggttattt caaagaagct ggagtggatg
    28801 ttgatttgaa attgccacca gaagaaagtt cttctgactt ggttatcaac ggaaaggcac
    28861 catttgcagt gtatttccaa gactacatgg ctaagaaatt ggaaaaagga gcaggaatca
    28921 ctgccgttgc agctattgtt gaacacaata catcaggaat catctctcgt aaatctgata
    28981 atgtaagcag tccaaaagac ttggttggta agaaatatgg gacatggaat gacccaactg
    29041 aacttgctat gttgaaaacc ttggtagaat ctcaaggtgg agactttgag aaggttgaaa
    29101 aagtaccaaa taacgactca aactcaatca caccgattgc caatggcgtc tttgatactg
    29161 cttggattta ctacggttgg gatggtatcc ttgctaaatc tcaaggtgta gatgctaact
    29221 tcatgtactt gaaagactat gtcaaggagt ttgactacta ttcaccagtt atcatcgcaa
    29281 acaacgacta tctgaaagat aacaaagaag aagctcgcaa agtcatccaa gccatcaaaa
    29341 aaggctacca atatgctatg gaacatccag aagaagctgc agatattctc atcaagaatg
    29401 cacctgaact caaggaaaaa cgtgactttg tcatcgaatc tcaaaaatac ttgtcaaaag
    29461 aatacgcaag cgacaaggaa aaatggggtc aatttgacgc agctcgctgg aatgctttct
    29521 acaaatggga taaagaaaat ggtatcctta aagaagactt gacagacaaa ggcttcacca
    29581 atgaatttgt gaaataatga cagaaattag actagagcac gtcagttatg cctatggtca
    29641 ggagaggatt ttagaggata tcaacctaca ggtgacttca ggcgaagtgg tttccatcct
    29701 aggcccaagt ggtgttggaa agaccaccct ctttaatcta atcgctggga ttttagaagt
    29761 tcagtcaggg agaattgtcc ttgatggtga agaaaatccc caggggcgcg tgagttatat
    29821 gttgcaaaag gatctgctct tggagcacaa gacggtgctt ggaaatatca ttctgcccct
    29881 cttgattcaa aaggtggata aggcagaagc tatttcccga gcggataaaa ttcttgcgac
    29941 cttccagctg acagctgtaa gagacaagta tcctcatgaa cttagcggtg ggatgcgcca
    30001 gcgtgtagcc ttactccgga cctacctttt tgggcacaag ctctttctct tagatgaggc
    30061 ctttagcgcc ttggatgaga tgacaaagat ggaactccac gcttggtacc ttgagattca
    30121 caagcagttg cagctaacaa ccttgattat tactcacagt atcgaggagg ccctcaatct
    30181 cagtgaccgc atctatatct tgaaaaatcg ccctgggcag attgtttcag aaattaaact
    30241 agattggtct gaagatgagg acaaggaagt tcaaaagatt gcctacaaac gtcaaatctt
    30301 ggcagaatta ggcttagata agtagaaaaa tagggagttg gtgaagatta tcctttacca
    30361 gcgccctttt tcttttaaaa atgagaaaat ttcggtataa tagtcaaaca aggtcaaggt
    30421 ttaaagagag aggtgggttt gttatgagat ttaaaaatac atcggatcat attgaggcct
    30481 acatcaaggc gattttagat caatctggta tcgtggagtt gcaacggagt cagttggcag
    30541 atacctttca ggttgttcct agtcagatta actacgtgat caagacacgc tttacggaaa
    30601 gtagaggcta cttggttgaa agtaagcgtg gtggcggagg ctacattcgt ataggacgga
    30661 ttgagttttc tagtcatcat gaaatgctcc gggagctgct ttactcgatt ggtgagcgag
    30721 tcagtcaaga aatttatgag gatattctcc agcttttggt tgagcaggaa ttgatgacca
    30781 agcaggagat gaatttgcta gaatcagtag ctttggatcg cgttttagga gaagaagctc
    30841 cagttgttcg agcaaacatg ctacgtcaga tcatacaaga ggtagataga aaagggaagt
    30901 aagatgaact attcaaaagc attgaatgaa tgtatcgaaa gtgcctacat ggttgctgga
    30961 cattttggag ctcgttatct agagtcgtgg cacttgttga ttgccatgtc taatcacagt
    31021 tatagtgtag caggggcaac tttaaatgat tatccgtatg agatggaccg tttagaagag
    31081 gtggctttgg aactgactga aacggactat agccaggatg aaacctttac ggaattgccg
    31141 ttctcccgtc gtttgcaggt tctttttgac gaagcagagt atgtagcgtc agtggtccat
    31201 gctaaggtgc tagggacaga gcacgtcttc tatgcgattt tgcatgatgg caatgccttg
    31261 gcaactcgta tcttggagag ggctggtttt tcttatgaag acaagaaaga tcaggtcaag
    31321 attgctaccc ttcgtcgaaa tttagaagaa cgtgcaggct ggactcgtga agatctcaag
    31381 gctttacgcc aacgccatcg tacagtagct gacaagcaaa attctatggc caatatgatg
    31441 ggcatgccgc agactcctag tggtggtctc gaggactata cgcatgattt gacagagcaa
    31501 gcgcgttctg gcaagttaga accagtcatc ggtcgggaca aggaaatctc acgtatgatt
    31561 caaatattga gccgtaagac taagaacaac cctgtcttgg ttggggatgc tggtgtcggg
    31621 aaaacagctc tggcccttgg tcttgctcag cgtattgcta gtggtgacgt gcctgcggaa
    31681 atggctaaga tgcgcgtgtt agaacttgat ttgatgaatg tcgttgcagg gacacgcttc
    31741 cgtggtgact ttgaagaacg catgaataat atcatcaagg atattgaaga agatggccaa
    31801 gtcatcctct ttatcgatga actccacacc atcatgggtt ctggtagcgg gattgattcg
    31861 actctggatg cggccaatat cttgaaacca gccttggcgc gtggaacttt gagaacggtt
    31921 ggtgccacca ctcaggaaga atatcaaaaa catatcgaaa aagatgcggc tctttctcgt
    31981 cgtttcgcta aagtgacgat tgaagaacca agtgtggcag atagtatgac tattttacaa
    32041 ggtttgaagg cgacttatga gaaacatcac cgtgtacaaa tcacagatga agcggttgaa
    32101 acagcggtta agatggctca tcgttattta accagtcgtc acttgccaga ctctgctatc
    32161 gacctcttgg acgaagcagc agcaacagtg caaaataagg caaagtatgt aaaagcagac
    32221 gattcagatt tgagtccagc tgacaaggcc ctgatggatg gcaagtggaa acaggcagcc
    32281 cagctaatcg caaaagaaga ggaagtacct gtctacaaag acttggtgac agagtctgat
    32341 attttgacca ccttgagtcg cttgtcagga atcccagttc aaaaactgac tcagactgac
    32401 gctaagaaat atctaaatct tgaagcagaa ctccataaac gggttatcgg tcaagatcaa
    32461 gctgtttcaa gtattagtcg tgcgattcgc cgtaatcagt caggaattcg cagtcacaag
    32521 cgtccgatcg gttcctttat gttcctagga cccacaggtg tcggtaagac cgagttggcc
    32581 aaggctttgg cagaagttct ctttgatgac gaatcagccc ttatccgctt tgatatgagt
    32641 gagtatatgg agaaattcgc agccagccgt ctaaatggag ctcctccagg ctatgtgggt
    32701 tacgaagaag gtggggagtt gacagagaag gttcgcaata aaccctattc cgttctcctc
    32761 tttgatgagg tagagaaggc tcacccagat atctttaatg ttctcttgca ggttctggat
    32821 gacggtgtct tgacagatag caagggacgc aaggtcgatt tttcaaatac cattatcatt
    32881 atgacatcga acctaggtgc gactgccctt cgtgatgata agactgttgg ttttggggct
    32941 aaggatattc gttttgacca ggaaaatatg gaaaaacgca tgtttgaaga actgaaaaaa
    33001 gcttatagac cggaattcat caaccgtatt gatgagaagg tggtcttcca tagcctatct
    33061 agtgaccata tgcaggaagt ggtgaagatt atggtcaagc ctttagtggc aagtttggct
    33121 gaaaaaggca ttgacttgaa attacaagct tcagctctga aattgttagc aaatcaagga
    33181 tatgacccag agatgggagc tcgcccactt cgcagaaccc tgcaaacaga agtggaggac
    33241 aagttggcag aacttcttct caagggagat ttagtggcag gcagcacact taagattggt
    33301 gtcaaagcag gccagttaaa atttgatatt gcataaaaga ataaaagtac cagcatctga
    33361 ccataagtca cagtggagtg aaactcaatg aaaatcaaag agcaaactag gcagctagcc
    33421 gcaggttgct caaaacactg gtttgaggtt gcagatagag ctgacgtggt ttgaagagat
    33481 tttcgaagag tataagctgc aagatgaatg attttcttgt attgacgttg ttgttgacaa
    33541 aaagtagcgg ataaatgaaa tccattccat tatcatagat gataggctgg taggaaattt
    33601 tcaaatagca tacaggaaat agatgtatgg agttctggta gtagaaaggg agagagatga
    33661 acattttagt tgcagatgac gaggaaatga ttagagaagg aattgcagca tttctgacag
    33721 aagagggtta tcatgtcatt atggctaagg atggacaaga ggtcttggaa aaatttcaag
    33781 atctccctat ccatctcatg gtactggatt taatgatgcc taggaagagt ggttttgaag
    33841 tgttaaaaga aatcaatcaa aagcacgata ttcctgtcat cgtcttgagt gctctgggag
    33901 atgaaattac tcagtcacag gtatttgatc tctatgctga tgatcatgtg acaaaacctt
    33961 tttctttggt actgcttgtc aagcgtatta aggcgcttat cagacgttac tacgtcatag
    34021 aggatatttg gcgatatcag gatgtaacag tggattttac ctcttacaaa gcacattata
    34081 aaaatgaaga aattgatctc aaaccaaagg aattactggt actaaagtgt ttgattcagc
    34141 ataaaaatca agttttaagt agagagcaga tattggaaga aatttcaaaa gatgtagctg
    34201 atttaccttg tgatagggtc gttgatgtct atattcgtac tcttcgcaaa aaattagctt
    34261 tagattgtat cgtgactgtg aaaaatgttg ggtataagat tagtttatga taaaaaatcc
    34321 taaattatta accaagtcct ttttaagaag ttttgcaatt ctaggtggtg ttggtctagt
    34381 cattcatata gctatttatt tgacctttcc tttttattat attcaactgg agggggaaaa
    34441 gtttaatgag agcgcaagag tgtttacgga gtatttaaag actaagacat ctgatgaaat
    34501 tccaagctta ctccagtctt attcaaagtc cttgaccata tctgctcacc ttaaaagaga
    34561 tattgtagat aagcggctcc ctcttgtgca tgacttggat attaaagatg gaaagctatc
    34621 aaattatatc gtgatgttag atatgtctgt tagtacagca gatggtaaac aggtaacagt
    34681 gcaatttgtt cacggggtgg atgtctacaa agaagcaaag aatattttgc ttttgtatct
    34741 cccatataca tttttggtta caattgcttt ttcctttgtt ttttcttatt tttatactaa
    34801 acgcttgctc aatcctcttt tttacatttc agaagtgact agtaaaatgc aagatttgga
    34861 tgacaatatt cgttttgatg aaagtaggaa agatgaagtt ggtgaagttg gaaaacagat
    34921 taatggtatg tatgagcact tgttgaaggt tatttatgag ttggaaagtc gtaatgagca
    34981 aattgtaaaa ttgcaaaatc aaaaggtttc ctttgtccgc ggagcatcac atgagttgaa
    35041 aaccccttta gccagtctta gaattatcct agagaatatg cagcataata ttggagatta
    35101 caaagatcat ccaaaatata ttgcaaagag tataaataag attgaccaga tgagccactt
    35161 attggaagaa gtactggagt cttctaaatt ccaagagtgg acagagtgtc gtgagacttt
    35221 gactgttaag ccagttttag tagatatttt atcacgttat caagaattag ctcattcaat
    35281 aggtgttaca attgaaaatc aattgacaga tgctaccaga gtcgtcatga gtcttagggc
    35341 attggataag gttttgacaa acctgattag taatgcaatt aaatattcag ataaaaatgg
    35401 gcgcgtaatc atatccgagc aagatggcta tctctctatc aaaaatacat gtgcgcctct
    35461 aagtgaccaa gaactagaac atttatttga tatattttat cattctcaaa tcgtgacaga
    35521 taaggatgaa agttccggtt tgggtcttta cattgtgaat aatattttag aaagctatca
    35581 aatggattat agttttctcc cttatgaaca cggtatggaa tttaagatta gcttgtagac
    35641 agattagttt tttattaaag ttcatatagg gttaacataa gtgtgttatt ctttgtgtag
    35701 ataaaagaaa ggatactaat atggtattag cgattatttt agtaacattc tttattcgat
    35761 tgattttttt aaagcgttcg atagagaatg agaaacgaat ccttagcaat ggcgggaaag
    35821 aatttggagt tgagaataca aaacgattaa ctatggctca tattgttttt tatctctctt
    35881 gcttggttga ggcaatggtg cacaagacaa tttttgatgg catgggcatg gttggtttag
    35941 tcttgcttat tttttctatg ctgatgttga tgttggtgat tcacttgttg ggagatattt
    36001 ggacagtgaa gcttatgctt gtcaataatc acaaatatgt agatcatatc ttgtttagga
    36061 cagtaaaaca ccctaattac tttttaaata ttttacctga gttgattggc ttgaccttgt
    36121 tgagtcatgc ctatatgact tttgttttag tttttccagt ttatgcagtt attttgtatc
    36181 gacgaatagc tgaagaggaa aagctattac atgaagttat aatcccaaat ggaagcataa
    36241 agagataaag agataaatac aaaattcgat ttatatacag ttcatattga agtgatatag
    36301 taaggttaaa gaaaaaatat agaaggaaat aaacatgttt gcatcaaaaa acgaaagaaa
    36361 agtacattat tcaattcgta aatttagtgt aggagtagct agcgtagctg ttgccagtct
    36421 ttttatggga agtgttgtac atgcgacaga gagagaggga agtaccccag tagccacttc
    36481 ttctaataag gcaaataaaa gtcagacaga acatatgaaa gctgctgaac aagtcgatga
    36541 atatataaac aaaatgatcc aattagataa aagaaaacat acccaaaatc tcgccttaaa
    36601 cataaagttg agcgcaatta aaacgaagta tttgcgtgaa ttaaatgttt tagaagagaa
    36661 gtcgaaaaaa gaagagttga cgtcaaaaac aaaaaaagag atagacgcag cttttgagca
    36721 gtttaacaaa gatacattga aaccaggaga aaaggttgaa gaagctcaga agaaggttga
    36781 agaagctgag aaaaaagcca aggatcaaaa agaagaagat caccgtaact acccaaccat
    36841 tacttacaaa acgcttgaac ttgaaattgc tgagtccgat gtggaagtta aaaaagcgga
    36901 gcttgaacta gtaaaagagg aagctaaggg atctcgaaac gaggaaaaaa ttaagaaagc
    36961 aaaagcggaa gttgagagta aaaaagctga ggctacaaag ttagaagaaa tcaagacaga
    37021 acgtaaaaaa gcagaagaag aagctaaacg aaaagcagaa gcagaagaag aagttaaaga
    37081 taaactaaag aagcggacaa aacgaggagc tcttggagag ccagcaacac ctgataaaaa
    37141 agaaaatgat gcgaagtctt cagattctag cgtaggtgaa gaaactcttc caagcccatc
    37201 cctgaaatca gaaaaaaaag tagcagaagc tgagaagaag gttgcagaag ctgagaaaaa
    37261 agccaaggat caaaaagaag aagatcgccg taactaccca accaatactt acaaaacgct
    37321 tgaacttgaa attgctgagt ccgatgtgaa agttaaagaa gcggagcttg aactagtaaa
    37381 agaggaagct aaggaacctc aaaacgagga aaaaattaag caagcaaaag cgaaagttga
    37441 gagtaaaaaa gctgaggcta caaggttaga aaaaatcaag acagatcgta aaaaagcaga
    37501 agaagctaaa cgaaaagtag cagaagaaga taaagttaaa gaaaaaccag ctgaacaacc
    37561 acaaccagct cctgcaccaa aaccagcgcc ggctcctcaa ccagaaaaac cagctgaaca
    37621 accaaaagca gaaaaaccag ctgatcaaca agctgaagaa gactatgctc gtagatcaga
    37681 agaagaatat aatcgcttga ctcaacagca accgccaaaa gcagaaaaac cagctcctgc
    37741 accacaacca gagcaaccag ctcctgcacc aaaaacaggt tggaaacaag aaaacggtat
    37801 gtggtacttc tacaatactg atggttcaat ggcaacaggt tggctccaaa acaatggttc
    37861 atggtactat ctaaacgcta atggttcaat ggcaacaggt tggctccaaa acaatggttc
    37921 atggtactac ctaaacgcta atggttcaat ggcaacaggt tggctccaaa acaatggttc
    37981 atggtactac ctaaacgcta atggtgatat ggcaacaggt tggctccaaa acaatggttc
    38041 atggtactac ctaaacgcta atggtgatat ggcgacaggt tggctccaat acaatggttc
    38101 atggtactac ctaaacgcta atggtgatat ggcgacaggt tggctccaat acaatggttc
    38161 atggtactac ctaaacgcta acggttcaat ggcaacagat tgggtgaaag atggagatac
    38221 ctggtactat cttgaagcat caggtgctat gaaagcaagc caatggttca aagtatcaga
    38281 taaatggtac tatgtcaatg gctcaggtgc ccttgcagtc aacacaactg tagatagcta
    38341 tagagtcaat gccaatggtg aatgggtaaa ctaaacctaa tataactagt taatactgac
    38401 ttcctgtaag aactctttaa agtattccct acaaatacca tatcctttca gtagataata
    38461 tacccttgta ggaagtttag attaaaaaat aactctgtaa tctctagccg gatttatggc
    38521 gctagagact acggagtttt tttgatgagg aaagaatggc ggcactcaag agactcttta
    38581 agagagttac gggttttaaa ctattaagcc ttctccaatt gcaagagggc ttcaatctct
    38641 gccagggtgc tggcttgcga aatggttcca cggagtttgg cagcgccaga tgttccacgg
    38701 aggtagtgag gagcgaggcc gcggaattca cgaactgcga cgttttctcc tttgaggtta
    38761 atcaatcgtt tcaagtgttc gtaggcgatc ttcatcttgt cttcaaaggt caaatcaggt
    38821 aggatttctc ctgtttcaaa gtaatggttg atttggttga agaggtaagg atttcccatg
    38881 gcagctcggc caatcatgac tgcgtcagca ccaacttctt cgatgcgttg cttggcttct
    38941 tggacagtac ggatatcacc gttggcgatg aatggaatct tggttagagc ttgggcaacc
    39001 ttgtaaaggg tctcaaggtc tgcgtggcca gtatacattt gttcacgggt acggccatgc
    39061 atggcgaggg cagaaacacc tgcagcctca gcagcgaggg cattttctac tgccagagat
    39121 gggtccgccc agccggtacg cattttgaca gtaagtggga tatcaaggac agactggacc
    39181 ttgttgatga tagagtagat cttgtcagga tccttgagcc acatagctcc agcttcgttc
    39241 ttcacgattt tgttgacagg gcagcccatg ttgatatcga cgatatcggt cttggtgttt
    39301 tcttggatga attctgctgc gcgtgctagg ctgtcttcat cgctaccaaa aagttggata
    39361 gagacagggt tttcgccctc atcgatatga agcatgtgca gggttttttc gttgttgtat
    39421 tggattccct tgtcagagac catttccatt acaacgagtc cagctccgag ctcttttgcg
    39481 atggtacgaa aggctgagtt ggtcacgcca gccataggcg ctaaaacggt acgattggga
    39541 atctcaacat tgccaatcat aaaaggtgta ttaagatttg tcacgaatga gttcctccag
    39601 gtccttttca tcaaagttgt aagtagtttg gcagaattga caagtgattt ctgcgccgtg
    39661 gtcttcctct ttcatttcct gtaaatctga gcttggaagg ctggcaagag cgttcataaa
    39721 gcgttcatgg ctacagtcac attggaaacg gatttcttct tcagaaagac gcttgtaggc
    39781 ttcgtccccg tagatagcct tgaggagggc ttcgatatgg tcgtcgcttt cgagaagagt
    39841 agagatagct ggcatttctt ggatgcgttt ttcaaagcga gcaatctctt ctttcttggc
    39901 tcctggcaag acttgaacta ggaaaccacc tgcaaccttg accttgtctt cctcgtccaa
    39961 aaggacattg aggccgaccg ctgaaggcgt ttgttggctt tcagtaaggt aaaaggcaag
    40021 gtcttcaccg atttctccag agatgagggg agttatagaa ttgtaaggat ttccagtacc
    40081 gtagtctgtg ataacgagga attgaccatt tccaacaaaa ggtccgacta ggacttcacc
    40141 agtcgcagtc tttttgatgt caacaccagg attttgaacg tagcctttga cgttcccctt
    40201 ggtatcagcg acggtgataa tagcacctag agagctagat cccaacacct taactgtaag
    40261 tttggtattt cctttttcat tggctgcgag aatctggctg gcgataagag ttcgaccaag
    40321 cgctacagtt gagctagctt gggtttgatg tttttcttga gcagtgcgga cggtttccgt
    40381 gctatcaagg acaaaagcac gaaaggctcc gctttctgat atagttttaa taattttatc
    40441 catagctact attttagcat aaaaatgccc aaagggggag ccgtgtgttt actgattttc
    40501 aggataatgg accaggaaat cagcatgaaa ataaaaagag aaacagatta tttcagcatt
    40561 tgtaagattt atgctatgct taaggtagaa aatgaaaggg gtaacaaatg tatttaggag
    40621 atttgatgga gaaagccgag tgtggtcaat tttcaatcct ttcctttcta ttacaagagt
    40681 ctcagacgac cgtcaaggct gtaatggaag aaacaggatt ttcaaaagca accctaacca
    40741 aatatgtcac cctgctcaat gacaaggctt tggatagtag cttagaactg actattcact
    40801 cagaagatga aaatctgcgt ctgtctatcg gtgcagctac caaggggaga gatattcgga
    40861 gcttgttttt ggagagtgct gttaaatacc agattttggt ttatcttctc taccaccaac
    40921 agtttttagc ccatcagctg gctcaagaat tggtgattag cgaggctacg cttggtcgtc
    40981 acttggctgg tttaaatcag attttgtcag aatttgattt atccatccaa aatggacgtt
    41041 ggcgaggtcc agagcatcag attcgctatt tctatttctg tcttttccga aaggtttggt
    41101 cgagtcagga atgggaaggt cacatgcaga aaacagagag aaaacaggag attgccaatt
    41161 tagaggaaat ctgcggtgca agtttgtctg cggggcagaa attggacttg gttctctggg
    41221 ctcacatcag tcaacaacgt cttcgggtca atgcctgtca gtttcaagtc atagaagaga
    41281 aaatgcgagg gtattttgac aatatcttct atcttcgttt gctgagaaag gttccgtcct
    41341 tttttgctgg gcaacatatt ccactaggag ttgaggatgg tgagatgatg atattcttct
    41401 cttttctcct atctcatcgc attcttcctc ttcatactat ggagtatatt cttggttttg
    41461 gagggcagtt ggcagattta ctgacgcaat tgattcaaga aatgaagaag gaggaactat
    41521 tgggggatta tacagaggac catgtcacct atgaactcag tcagctttgt gctcaagtct
    41581 atctctataa gggctatatt ttacaggatc gctacaagta ccagttagag aatcgtcatc
    41641 catatttact gatggaacat gattttaaag agacagcaga ggagattttt catgctctac
    41701 ctgcttttca acaggggaca gatttagata agaagattct ctgggaatgg ctccagttaa
    41761 tcgaatatat ggctgaaaac ggtggccagc atatgcggat tggtctggat ttgacatctg
    41821 gttttcttgt cttttcaagg atggcagcca ttttgaaacg gtatttggaa tacaatcgtt
    41881 ttatcaccat tgaagcttat gaccctagtc ggcattatga tttgctggtt accaataacc
    41941 cgattcataa gaaggaacag acaccagtct attatttaaa aaatgacttg gatatggagg
    42001 atttggcagg gattcgtcag ttattattca cttaaaaggc ttggttaatt caggtctttt
    42061 ttgtgaaatt cacacaatct cctcacattt ttttaaaaat taaaaaaagt tgataaacaa
    42121 gaaagcgctt tattttgtat actagtaagt gtaaagagga aacacctcaa gatctttatc
    42181 aggaggacag tatatgtcac aagaaaaata catcatggcc attgaccagg gaactacaag
    42241 ttctcgtgcc atcattttca acaaaaaagg ggaaaaggtt agctcgagtc aaaaagagtt
    42301 tacccagatt ttccctcagg caggttgggt tgagcacaat gccaatgaaa tttggaactc
    42361 tgttcagtca gttattgcgg gtgctttcat cgaaagtggt gtcaagccaa atcaaatcga
    42421 ggcaatcggg attaccaacc aacgtgaaac aacggttgtc tgggataaga aaacaggact
    42481 tcctatctac aatgctatcg tttggcagtc acgccagaca gcacctttgg ctgagcaact
    42541 aaaaagccaa ggttatgtgg aaaaattcca tgaaaagact ggtttgatta ttgatgctta
    42601 cttctctgct accaaggttc gttggatttt ggatcatgta gaaggcgctc aagagcgagc
    42661 agaaaaaggg gaattgctct ttggtactat cgatacttgg ttggtttgga aattgactga
    42721 cggtgcggct cacgtgactg actactcaaa tgcagctcgt accatgcttt ataacattaa
    42781 agaactcaaa tgggatgatg aaattttgga aatccttaac attccgaagg ctatacttcc
    42841 agaagttcgt tctaactccg aaatctacgg caagacagct ccattccatt tctacggtgg
    42901 agaggtgcca atctcaggta tggctgggga ccaacaagca gccctctttg gacagttggc
    42961 ttttgagcca ggtatggtta agaatactta tggaacaggc tctttcatca tcatgaatac
    43021 tggggaagag atgcagttgt ctgaaaacaa cctcttgaca accattggtt acggaatcaa
    43081 cggtaaggtt tattatgcct tggaaggttc tatcttcatc gcaggaagtg ctattcagtg
    43141 gcttcgtgac ggtcttcgca tggttgaaaa ttcaccagaa tctgaaaaat acgctcgtga
    43201 ttctcacaac aacgatgaag tttatgtcgt tccagccttt acaggtctag gcgctccata
    43261 ctggaaccaa aatgctcgtg gttccgtctt tggtttgact cgtggaacaa gcaaagaaga
    43321 ctttatcaag gcgactttgc aatctattgc ttatcaagtg cgtgacatca tcgacaccat
    43381 gcaagtggat actcagaccg ccattcaagt attgaaggtg gatggtggtg cagccatgaa
    43441 caacttcctc atgcagttcc aggcggatat tttaggcatt gatattgcac gtgctaaaaa
    43501 cttggaaaca acagctctag gagcggcctt cctagcaggt ttatcagtag ggtactggaa
    43561 agacttggac gagttgaaac tcttgaacga gacaggagaa ctctttgagc catctatgaa
    43621 tgaatctcgc aaggaacaac tctacaaggg ctggaagaag gctgtgaaag caactcaagt
    43681 ctttgcggaa gtagacgact aatactggca gaataaagcg atttatttag aaagtgtgta
    43741 aatatggaat tttcaaagaa aacacgtgaa ttgtcaatta aaaaaatgca ggaacgcacc
    43801 ctggacctct tgattatcgg tggaggaatc acaggggctg gtgtagcctt gcaggcggca
    43861 gctagcggtc ttgagactgg tttgattgaa atgcaagatt ttgcagaagg aacatctagt
    43921 cgttcaacaa aattggttca cggaggactt cgttacctta aacaattcga cgtagaagtg
    43981 gtctcagata cggtttctga acgtgcagtg gttcaacaaa tcgctccaca cattccaaaa
    44041 tcagacccaa tgctcttacc agtttacgat gaagatggag cgacctttag cctcttccgt
    44101 ctcaaagtag ccatggactt gtacgacctt ttagcaggtg tgagcaacac gtcagctgcg
    44161 aacaaggttt tgagcaagga tcaagtcttg gaacgccagc caaacttgaa gaaggaaggc
    44221 ttggtaggag gtggagtgta tcttgacttc cgtaacaacg atgcgcgtct cgtgattgaa
    44281 aacatcaaac gtgccaacca agacggtgcc ctcattgcca accacgtgaa ggcagaaggc
    44341 ttcctctttg acgaaagtgg caagattaca ggtgttgtag ctcgtgatct cttgacagac
    44401 caagtatttg aaatcaaggc ccgtctggtt attaatacaa caggtccttg gagtgataaa
    44461 gtacgtaatt tgtctaataa gggaacgcaa ttctcacaaa tgcgcccaac taagggagtt
    44521 cacttggtag tggattcaag caaaatcaag gtttcacagc cagtttactt cgacacaggt
    44581 ttgggtgacg gtcgtatggt ctttgttctc ccacgtgaaa acaagactta ctttggtaca
    44641 actgatacag actacacagg tgatttggag catccaaaag taactcaaga agatgtagat
    44701 tatctacttg gcattgtcaa caaccgcttt ccagaatcca acatcaccat tgatgatatc
    44761 gaaagcagct gggcaggtct tcgtccattg attgcaggga acagtgcctc tgactataat
    44821 ggtggaaata acggtaccat cagtgatgaa agctttgaca acttgattgc gactgttgaa
    44881 tcttatctct ccaaagaaaa aacacgtgaa gatgttgagt ctgctgtcag caagcttgaa
    44941 agtagcacat ctgagaaata tttggatcca tctgcagttt ctcgtgggtc tagcttggac
    45001 cgtgatgaca atggtctttt gactcttgct ggcggtaaaa tcacagacta ccgtaagatg
    45061 gctgaaggag ctatggagcg cgtggttgac atcctcaaag cagaatttga ccgtagcttt
    45121 aaattgatca attctaaaac ttaccctgtt tcaggtggag aattgaaccc agcaaatgtg
    45181 gattcagaaa tcgaagcctt tgcgcaactt ggagtatcac gtggtttgga tagcaaggaa
    45241 gctcactatc tggcaaatct ttacggttca aatgcaccga aagtctttgc acttgctcac
    45301 agcttggaac aagcgccagg actcagcttg gcagatacct tgtcccttca ctatgcaatg
    45361 cgcaacgagt tggctcttag cccagttgac ttccttcttc gtcgtaccaa ccatatgctc
    45421 tttatgcgtg atagcttgga tagcatcgtt gagccagttt tggatgaaat gggacgattc
    45481 tatgactgga cagaagaaga aaaagcaact taccgtgctg atgtcgaagc agctctcgct
    45541 aacaacgatt tagcagaatt aaaaaattaa gaaaaaataa aagaggtgga gggcagcatt
    45601 ccttgtcgcc cgtcccttct ttttaatgga gacagaaaga tgatgaatga attatttgga
    45661 gaatttctag ggactttaat cctgattctt ctaggaaatg gtgttgttgc aggtgtggtt
    45721 cttcctaaaa ccaagagcaa tagctcaggt tggattgtga ttactatggg ttgggggatt
    45781 gcagttgcgg ttgcagtctt tgtatctggc aagctcagtc cagctcattt aaatccagct
    45841 gtgacagttg gtgtagcttt aaaaggtgac ttgccttggg cttccgtttt gccttatatc
    45901 ttagcccagt tcgcaggggc catgctgggt cagattttgg tttggttgca attcaaacct
    45961 cactatgagg cagaagaaaa tgcaggcaat atcctggcaa ccttcagtac tggaccagcc
    46021 atcaaggata ctgtatcaaa cttgattagc gaaatccttg gaacctttgt tttggtgttg
    46081 acaatctttg ctttgggtct ttacgatttt caggcaggta tcggaacctt tgcagtggga
    46141 actttgattg tcggtatcgg tctatcacta ggtgggacaa caggttatgc cttgaaccca
    46201 gctcgtgacc ttggacctcg tatcatgcac agcatcttgc caattccaaa caagggagac
    46261 ggagactggt cttacgcttg gattcctgtt gtaggccctg ttatcggagc agccttggca
    46321 gtgcttgtat tctcactttt ctagtttata ctcttcgaaa atcaaattca aaccacgtca
    46381 gcgtcgcctt accgtactca agtacagctt gcggctagct tcctagtttg ctctttgatt
    46441 ttcattgagt attagaaaac aattatgttg atagagcttg ggcaagagcc caatttcagc
    46501 aaaaaatgaa gtaaatcttc tcataataaa acgcatcata tcaagcacga aaattccacg
    46561 aggtcaacta cagtcagaaa gctgaacaac aagccaaaac gcccaaaaaa ggcggcaaaa
    46621 agcaagcacc tgcaagcaac gtgccgaaat ggtcaaatcc tgattatgtc aacgaattag
    46681 acccaaaaat cgttgatatg ctagtagaat ttcacaagtc acaaggcact ttggaaactc
    46741 ccgaggcgca agcagaaatc gcccaaaaac gtgaagaaat cgagcaaagg agagctgagc
    46801 ttgagggtaa aaaacaagag cttttgaacc gcttgaacaa atagagtttc gcaagtatta
    46861 tgcttacaaa ttacttgagc aattaactaa aatataaact ctgcctagat ataaaggcag
    46921 ggtttatatt ttagaaattc acgtaggttg ttacggtttt tacataccca gtatagtttg
    46981 agtttctata gtattcagtg ataaacttcc attttctttg agcaacatgg atataagtac
    47041 ttgttatgta gtatggatat gggctttgtg aatccaagta agactgataa gcttgtatac
    47101 caaaatatgc tccaccaatt attgcacccc atggaccccc caataaagca cctatcctac
    47161 caatcatata actgattcca gcaccagtca tgaagttagc gaatgtgtta gcttgtttat
    47221 tcccatgtat tgtgttgacg taattccaaa cattaggatc gtatgatcta aaagatatat
    47281 ttaggtcgat ttcattcttt tgataagcca tataaaatgc cccattgata tagacgccgt
    47341 cagcacgtcg ttcaatagtg tctacacttc catctggatt gacaacctca agaacttcat
    47401 cgcttaaaat atttacttgc gtatctccga accgcactga tgagccattc tcaaactgag
    47461 cctcaccaga tacaacttta gagtttgccg ataagctatc atcagcaaaa acaacaagcg
    47521 acggggaaat gctagacata cagaaaacag acataactag caaacacatg catttaaaca
    47581 tcttagacat aacggaaact cttttgtatt tttgat
//
LOCUS       DAVLYR010000003        45094 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLYR010000003 DAVLYR010000000
VERSION     DAVLYR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.003"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            403..636
                     /locus_tag="VYO63_000150"
     CDS             403..636
                     /locus_tag="VYO63_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1671138.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            919..2868
                     /gene="mutL"
                     /locus_tag="VYO63_000151"
     CDS             919..2868
                     /gene="mutL"
                     /locus_tag="VYO63_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1671139.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3193..3660)
                     /locus_tag="VYO63_000152"
     CDS             complement(3193..3660)
                     /locus_tag="VYO63_000152"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1671140.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIAVAWIPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3661..4866)
                     /locus_tag="VYO63_000153"
     CDS             complement(3661..4866)
                     /locus_tag="VYO63_000153"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1671141.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(4886..5521)
                     /gene="ribE"
                     /locus_tag="VYO63_000154"
     CDS             complement(4886..5521)
                     /gene="ribE"
                     /locus_tag="VYO63_000154"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1671142.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5506..6606)
                     /gene="ribD"
                     /locus_tag="VYO63_000155"
     CDS             complement(5506..6606)
                     /gene="ribD"
                     /locus_tag="VYO63_000155"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1671143.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(6741..6855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7012..7605
                     /gene="ruvA"
                     /locus_tag="VYO63_000156"
     CDS             7012..7605
                     /gene="ruvA"
                     /locus_tag="VYO63_000156"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1671144.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7615..8178
                     /locus_tag="VYO63_000157"
     CDS             7615..8178
                     /locus_tag="VYO63_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1671145.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8175..8852
                     /locus_tag="VYO63_000158"
     CDS             8175..8852
                     /locus_tag="VYO63_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1671146.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIATYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVDYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9007..10038)
                     /locus_tag="VYO63_000159"
     CDS             complement(9007..10038)
                     /locus_tag="VYO63_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1671147.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKVLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEEPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10293..10976)
                     /locus_tag="VYO63_000160"
     CDS             complement(10293..10976)
                     /locus_tag="VYO63_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1671148.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(10988..11932)
                     /locus_tag="VYO63_000161"
     CDS             complement(10988..11932)
                     /locus_tag="VYO63_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1671149.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12064..14895
                     /gene="uvrA"
                     /locus_tag="VYO63_000162"
     CDS             12064..14895
                     /gene="uvrA"
                     /locus_tag="VYO63_000162"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1671150.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            14888..15949
                     /locus_tag="VYO63_000163"
     CDS             14888..15949
                     /locus_tag="VYO63_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1671151.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLFPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16081..16479
                     /gene="spx"
                     /locus_tag="VYO63_000164"
     CDS             16081..16479
                     /gene="spx"
                     /locus_tag="VYO63_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1671152.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16545..17114
                     /locus_tag="VYO63_000165"
     CDS             16545..17114
                     /locus_tag="VYO63_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1671153.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17201..17467
                     /locus_tag="VYO63_000166"
     CDS             17201..17467
                     /locus_tag="VYO63_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1671154.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17471..17890
                     /gene="ruvX"
                     /locus_tag="VYO63_000167"
     CDS             17471..17890
                     /gene="ruvX"
                     /locus_tag="VYO63_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1671155.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            17906..18211
                     /locus_tag="VYO63_000168"
     CDS             17906..18211
                     /locus_tag="VYO63_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1671156.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18624..19874
                     /locus_tag="VYO63_000169"
     CDS             18624..19874
                     /locus_tag="VYO63_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1671157.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            19871..20428
                     /locus_tag="VYO63_000170"
     CDS             19871..20428
                     /locus_tag="VYO63_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1671158.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            20574..22106
                     /gene="cls"
                     /locus_tag="VYO63_000171"
     CDS             20574..22106
                     /gene="cls"
                     /locus_tag="VYO63_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1671159.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGINLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22184..22327
                     /locus_tag="VYO63_000172"
     CDS             22184..22327
                     /locus_tag="VYO63_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671160.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            22364..23902
                     /locus_tag="VYO63_000173"
     CDS             22364..23902
                     /locus_tag="VYO63_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1671161.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFEFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24022..26229
                     /gene="nrdD"
                     /locus_tag="VYO63_000174"
     CDS             24022..26229
                     /gene="nrdD"
                     /locus_tag="VYO63_000174"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1671162.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26241..26381
                     /locus_tag="VYO63_000175"
     CDS             26241..26381
                     /locus_tag="VYO63_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671163.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26425..26931
                     /locus_tag="VYO63_000176"
     CDS             26425..26931
                     /locus_tag="VYO63_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671164.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNLASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            26924..27514
                     /gene="nrdG"
                     /locus_tag="VYO63_000177"
     CDS             26924..27514
                     /gene="nrdG"
                     /locus_tag="VYO63_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1671165.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27511..28128
                     /locus_tag="VYO63_000178"
     CDS             27511..28128
                     /locus_tag="VYO63_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1671166.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQASMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28394..28702
                     /gene="rpsJ"
                     /locus_tag="VYO63_000179"
     CDS             28394..28702
                     /gene="rpsJ"
                     /locus_tag="VYO63_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1671167.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            28919..29545
                     /gene="rplC"
                     /locus_tag="VYO63_000180"
     CDS             28919..29545
                     /gene="rplC"
                     /locus_tag="VYO63_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1671168.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29570..30193
                     /gene="rplD"
                     /locus_tag="VYO63_000181"
     CDS             29570..30193
                     /gene="rplD"
                     /locus_tag="VYO63_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1671169.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30193..30489
                     /locus_tag="VYO63_000182"
     CDS             30193..30489
                     /locus_tag="VYO63_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1671170.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30507..31340
                     /gene="rplB"
                     /locus_tag="VYO63_000183"
     CDS             30507..31340
                     /gene="rplB"
                     /locus_tag="VYO63_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1671171.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31444..31725
                     /gene="rpsS"
                     /locus_tag="VYO63_000184"
     CDS             31444..31725
                     /gene="rpsS"
                     /locus_tag="VYO63_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1671172.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            31737..32081
                     /gene="rplV"
                     /locus_tag="VYO63_000185"
     CDS             31737..32081
                     /gene="rplV"
                     /locus_tag="VYO63_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1671173.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32094..32747
                     /gene="rpsC"
                     /locus_tag="VYO63_000186"
     CDS             32094..32747
                     /gene="rpsC"
                     /locus_tag="VYO63_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1671174.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            32751..33164
                     /gene="rplP"
                     /locus_tag="VYO63_000187"
     CDS             32751..33164
                     /gene="rplP"
                     /locus_tag="VYO63_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1671175.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33174..33380
                     /locus_tag="VYO63_000188"
     CDS             33174..33380
                     /locus_tag="VYO63_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1671176.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33405..33665
                     /gene="rpsQ"
                     /locus_tag="VYO63_000189"
     CDS             33405..33665
                     /gene="rpsQ"
                     /locus_tag="VYO63_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1671177.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            33691..34059
                     /gene="rplN"
                     /locus_tag="VYO63_000190"
     CDS             33691..34059
                     /gene="rplN"
                     /locus_tag="VYO63_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1671178.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34137..34442
                     /gene="rplX"
                     /locus_tag="VYO63_000191"
     CDS             34137..34442
                     /gene="rplX"
                     /locus_tag="VYO63_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1671179.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34466..35008
                     /gene="rplE"
                     /locus_tag="VYO63_000192"
     CDS             34466..35008
                     /gene="rplE"
                     /locus_tag="VYO63_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1671180.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35026..35295
                     /gene="rpsN"
                     /locus_tag="VYO63_000193"
     CDS             35026..35295
                     /gene="rpsN"
                     /locus_tag="VYO63_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1671181.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35580..35978
                     /gene="rpsH"
                     /locus_tag="VYO63_000194"
     CDS             35580..35978
                     /gene="rpsH"
                     /locus_tag="VYO63_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1671182.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36170..36706
                     /gene="rplF"
                     /locus_tag="VYO63_000195"
     CDS             36170..36706
                     /gene="rplF"
                     /locus_tag="VYO63_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1671183.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            36790..37146
                     /gene="rplR"
                     /locus_tag="VYO63_000196"
     CDS             36790..37146
                     /gene="rplR"
                     /locus_tag="VYO63_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1671184.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37164..37658
                     /gene="rpsE"
                     /locus_tag="VYO63_000197"
     CDS             37164..37658
                     /gene="rpsE"
                     /locus_tag="VYO63_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1671185.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37630..37854
                     /gene="rpmD"
                     /locus_tag="VYO63_000198"
     CDS             37630..37854
                     /gene="rpmD"
                     /locus_tag="VYO63_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1671186.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            37999..38439
                     /gene="rplO"
                     /locus_tag="VYO63_000199"
     CDS             37999..38439
                     /gene="rplO"
                     /locus_tag="VYO63_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1671187.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38452..39762
                     /gene="secY"
                     /locus_tag="VYO63_000200"
     CDS             38452..39762
                     /gene="secY"
                     /locus_tag="VYO63_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1671188.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            39913..40551
                     /locus_tag="VYO63_000201"
     CDS             39913..40551
                     /locus_tag="VYO63_000201"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1671189.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40668..40886
                     /gene="infA"
                     /locus_tag="VYO63_000202"
     CDS             40668..40886
                     /gene="infA"
                     /locus_tag="VYO63_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1671190.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            40911..41027
                     /gene="rpmJ"
                     /locus_tag="VYO63_000203"
     CDS             40911..41027
                     /gene="rpmJ"
                     /locus_tag="VYO63_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1671191.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41045..41410
                     /gene="rpsM"
                     /locus_tag="VYO63_000204"
     CDS             41045..41410
                     /gene="rpsM"
                     /locus_tag="VYO63_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1671192.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41428..41811
                     /gene="rpsK"
                     /locus_tag="VYO63_000205"
     CDS             41428..41811
                     /gene="rpsK"
                     /locus_tag="VYO63_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1671193.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            41854..42789
                     /locus_tag="VYO63_000206"
     CDS             41854..42789
                     /locus_tag="VYO63_000206"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1671194.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            42801..43187
                     /gene="rplQ"
                     /locus_tag="VYO63_000207"
     CDS             42801..43187
                     /gene="rplQ"
                     /locus_tag="VYO63_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1671195.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43452..43718
                     /locus_tag="VYO63_000208"
     CDS             43452..43718
                     /locus_tag="VYO63_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1671196.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            43728..45065
                     /locus_tag="VYO63_000209"
     CDS             43728..45065
                     /locus_tag="VYO63_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1671197.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        13600 a         8562 c         9919 g        13013 t
ORIGIN      
        1 gcataatgac tataactgaa aaagaaaagt gatatcttta aggttgttct tcttggtggt
       61 gagattcgtg agacaaccca agcttttgtc ggaaagatta ccaatgcttt gatggatagg
      121 atgtacttta gcaagatgtt tttagtggta acggtatcgt ggatggacgt gtaataacct
      181 cttctttcga ggagtatttt actaaaaaac tagccttgga gcgttcccca gaaacggact
      241 tactcattga ctcttcaaag atttggggag aagattttgc ttcatctgtt ccttgaaaaa
      301 agtcacagca gtcatcacag acgatagtac tgaacaaaac tatgaagagt tagaaattta
      361 tacgcaggtg attgtataaa ggatctggaa atagataaga agttgattag tattgaccta
      421 ggtggtacaa atattaagat tactgttctt tcaaatgacg gtgagattga aactttgtgg
      481 agtattacaa cagatacaag tgagaaaggt tctcaaatta tatcggacat catcagttct
      541 attaaaaata aattgaccga acggaatatt cctgatagcg accttcttgg aatcggtatg
      601 ggaagttgct catcatactt tccttgtaaa tcataggggc tataaactct ccgtctactt
      661 gtcctgcaac aattgaagtc tgctcaaaac gccgtccgct aatcttttca tagactttct
      721 cccttttagg agcctagctt tctagtttgt tctttgattt ttattgagta taccactatt
      781 ttactccctc tggcaaggga ctttgtctat gtggagggat tgggctccta tgtggtggag
      841 cttttctgtt ctttctgaaa tatggtataa tagcactaat caatttctag gaaaatagat
      901 acagaaaggg gctgaaagat gtctcatatt attgaattgc cagagatgct ggcaaaccaa
      961 atcgcggctg gagaggtcat tgaacgtcct gccagtgtgg tcaaagagtt ggtagaaaat
     1021 gccattgacg cgggctctag tcagattatc attgagattg aggaagctgg tctcaagaag
     1081 gttcaaatca cggataacgg tcatggaatt gcccacgatg aggtggagtt ggccctgcgt
     1141 cgccatgcga ccagtaagat aaaaaatcaa gcagatctct ttcggattcg gacgcttggt
     1201 tttcgtggtg aagccttgcc ttctattgcg tctgttagtg tcttgactct gttaacggcg
     1261 gtggatggtg ctagtcatgg aaccaagtta gtcgcgcgtg ggggtgaagt tgaggaagtc
     1321 atcccagcga ctagtcctgt gggaaccaag gtttgtgtgg aggatctctt tttcaacacg
     1381 cctgcccgtc tcaagtatat gaagagccag caagcggagt tgtctcatat cattgatatt
     1441 gtcaaccgtc tgggcttggc ccatcctgag atttctttta gcttgattag tgatggcaag
     1501 gaaatgacgc ggacagcagg gactggtcaa ctgcgccaag caatcgcagg aatttacggt
     1561 ttggtcagtg ccaagaagat gattgaaatt gagaactctg acctagattt cgaaatttca
     1621 ggttttgtgt ccttgcctga gttgactcgg gctaaccgca attatatcag cctcttcatc
     1681 aatggccgtt atattaagaa cttcctgctc aatcgtgcta ttttggatgg ttttggaagc
     1741 aagcttatgg ttggacgttt tccactggct gtcattcaca tccatatcga cccttatcta
     1801 gcggatgtca atgtgcatcc aactaagcaa gaggtgcgga tttccaagga aaaagaactg
     1861 atgactctgg tttcagaagc tattgcaaat agtctcaagg aacaaacctt gattccagat
     1921 gccttggaaa atcttgccaa atcgaccgtg cgcaatcgtg agaaggtgga gcaaactatt
     1981 ctcccactca aagaaaatac gctctactat gagaaaactg agccgtcaag acctagtcaa
     2041 actgaagtag ctgattatca ggtagaattg actgatgaag ggcaggattt gaccctgttt
     2101 gccaaggaaa ccttggaccg attgaccaag ccagcaaaac tgcattttgc agagagaaag
     2161 cctgctaact acgaccagct agaccatcca gagttagatc ttgctagcat cgataaggct
     2221 tatgacaaac tggagcgaga agaagcatcc agcttcccag agttggagtt tttcggacaa
     2281 atgcacggga cttatctctt tgcccaaggg cgagatggac tttacatcat agatcagcac
     2341 gctgctcagg aacgggtcaa gtacgaggag taccgtgaaa gcattggcaa tgttgaccag
     2401 agccagcagc aactcctagt gccctatatc tttgaatttc ctgcggatga tgccctgcgt
     2461 ctcaaggaaa gaatgcctct cttagaggaa gtgggcgtct ttctagcaga gtacggagaa
     2521 aatcaattta ttctacgtga acatcctatt tggatggcag aagaagagat tgaatcaggc
     2581 atctatgaga tgtgcgacat gctccttttg accaaggaag tttctatcaa gaaataccga
     2641 gcagagctgg ctatcatgat gtcttgcaag cgatctatca aggccaatca tcgtattgat
     2701 gatcattcag ctagacaact cctctatcag ctttctcaat gtgacaatcc ctataactgt
     2761 cctcacggac gtcctgtttt ggtgcatttt accaagtcgg atatggaaaa gatgttccga
     2821 cgtattcagg aaaatcacac cagtctccgt gagttgggga aatattaaaa gtataaaaaa
     2881 gtctgggaaa aattttcaaa atcagaaaaa cgcataaaat caggtgttca aaaaccttga
     2941 ttttatgcgt tttatcatag aaatatttac ttcatttttt cctaattctt ttcgaaactc
     3001 tttttaaacg acgtcagttt tatcagtaat ctcaaaacag tgttttgagc taattttgcc
     3061 agttttgtct gtaacatcga agttgtgttt taccactctg cgactggttt cctagtttgc
     3121 tctatgattt tcacagagta ttaaattgcg attttgccaa gtttctttat tcgtctaaaa
     3181 gtagagtctg ttctatgcgt ctaatgtacg aattaggttg accatttcaa tagctccttg
     3241 tgcacactca gaacccttat ttcctgcttt agtgccagct cgttctatgg cttgttcaat
     3301 tgtatctgtc gttagcacac caaacataac aggaatttcg ctatttaaac tgatttgggc
     3361 gattccctta gatacctcgc tacatacata atcataatga cttgtattcc ctctaatgac
     3421 agctcccaag cagataattg catcatattt tttacttttt gccatttttg atgcaatcag
     3481 tggtatttca aaagctcctg gaatccaggc taccgctata tctttctcgt ttacattctc
     3541 tcttttgaga ttatctagtg ctccagataa taattttgaa gttataaatt cattaaatct
     3601 cgctacaaca atacctattt taatattgtt tgctactaaa ttaccttcat aagtgttcat
     3661 ttatttttcc tccatattta aaatgtgacc cattcgattt ttctttgttt ctaaataaaa
     3721 actatcgtaa ggattggctt ctatttcgat tgatattcta ctggaaatgg taattccata
     3781 tttttctaac tgttcaacct tgtcaggatt atttgtcagt aaatgaagtg actgaagtcc
     3841 cagatcttta agcatttgtg ctccaatatg atattctctt aaatcacctt caaagcctaa
     3901 tgcaagattg gcatcaagcg tatccatgcc ttgatcttgt aaatgatagg cttttaattt
     3961 attgataagt ccaattcctc gtccctcctg tcgcaagtaa agtaagacac ccgaaccatt
     4021 ctcaacaatc attttcatag ctttatcgaa ttgctgtcca caatcgcaac gtaaagagcc
     4081 taaaacatcc cctgttaaac attcggagtg gacccgacat aatacattgg cttcatcctc
     4141 tatgtttccc ataataagag caagatgatg ttccccattt agtttatcta tatagctaat
     4201 tgctttgaaa ttaccgtatc tagtaggcat attgacagtt gaaactcgtt ctaccagctg
     4261 atcatatact tttctatatt cttgtaattc tttgatggta attagtggaa tgttgtgttt
     4321 cttcgagaac tgaattaaat catctgttct catcattttg ccatcatgat tcattatttc
     4381 acaacatagg ccacactctt ttagtccagc taattttaat aaatcaacag ttgcttctgt
     4441 gtgtccattt ctttctagaa caccaccttt ttttgcaatt aaaggaaaca tgtgtcctgg
     4501 cctgcgaaaa tcagagggtg ttatatcttc agctacacac atacgtgcgg tcagtcctct
     4561 ttcctcggca gaaatacctg tggtcgtttc tttataatca attgaaactg taaaagcagt
     4621 cttatgatta tctgtattgt tttcaaccat aggtgaaagc attaattgat tagctaaact
     4681 ttcgctcata ggcatacaaa ttaatccttt ggcataagta gccataaaat taacattttc
     4741 tgttgtagct gcttgtgcag aacaaattaa gtctccttca ttttctctat ccttgtcgtc
     4801 tataacaaga acaagtcgtc ccttctgcaa tgcttctaat gcttcttgta tttttcgata
     4861 ttccattgac tgattatcct ttctgctaaa atccattttg atataatagt tccttagata
     4921 tttctgattt tggagagtta tccatcagtt tttgcacata tttacctaag atatcatttt
     4981 caagatttac tgtactcccg acttgtttac tcttaagaat ggtttgttcc aaggtatgag
     5041 ggataacaga tactgaaaag tttactttgg agactttagc gacagtcaga ctaatgccgt
     5101 caattgtaat agatcctttt tcaactatta aatctaaaat ttctttttgt gtgttgattt
     5161 gataccatac agcattatca tcttttttta ttgacgagat ttttcctgta ccatcaatgt
     5221 gtcctgtaac gacgtgaccc ccaagtcgac cgttgacaga taaggctctt tctagattca
     5281 cctcacttcc atgttttaat agagtaagag ctgttcgact ccatgtttca ttcattacat
     5341 caactgtaaa ggattgatgg ttgaaatgag taactgtaag acagatacca tttactgcta
     5401 tactatcgcc taaatggata tccgttaata tttttgaggc tttaattgat agtttacaat
     5461 tacgagagtc tttctgtatt ctttcaactt ttccgatttc ttcaattatt cctgtgaaca
     5521 tggataaatc acttcacttt ctatgagata gtcatttcct atttgagaaa aagcataagg
     5581 tttcaatcta atagcgtcat ttggcaaaga aatgccttca cctccgacag gaaacttggc
     5641 actgcctcca aaaatttttg gtgcaatata tattttcagc tcatcaacaa tttgttgttc
     5701 caaagcactc caattcatta gactgccccc ttctagaact aggctatcaa tctgcatgtt
     5761 tcctagatgt tgcattaaac tcgataagtc tatatgattg ccttttttct ttatggaaag
     5821 tatttcacag ccatgatttt gatatagctt cattttattt ttgtcttcag aggaagtggc
     5881 aatgtaagtt ttaatatcat ttgctgtttt tacgatttta gaggtaagag gagttcgtaa
     5941 atgtgtatcg catatgatac ggataggatt tttcccttcc tccaatctac atgtcagcaa
     6001 aggatcgtct tgaataacag tattgactcc caccataatt gcactaacat agtgtcgtaa
     6061 ctgatgcaca tgctttcttg cttcttcttc agtaatccat ttggattgat ttgttttagt
     6121 ggctattttt ccatccattg acattgcata tttcataaaa acatagggta catgctgggt
     6181 aatatacttt ctaaaacttt ttattaagtt aagacactca ttttctaaaa ttccaacagt
     6241 aacttgaaga ttattttcct caagtatctt tactcctttt ccagatacaa taggattaca
     6301 gtctaggctt ccaatgacta ctcttgtaat accactatcg attatagcat ctatacaggg
     6361 aggtgttttc ccgaagtgac aacagggttc aagtgttaca taaagcgtcg ctccgacagg
     6421 ggattctcta cagtttttaa gagcatttct ctcagcatgt gggccaccaa aaaactcatg
     6481 ataaccttgt ccgatgatgt gattatcttt tacaataact gcgccgacca taggattggg
     6541 attgacgtaa ccagcccctt tttgtgccag ttttattgct aatttcatat attttgaatc
     6601 gctcatctca ctacctccaa aaaaaatata ccttgaatag gggactactc aaggcataca
     6661 aaagaaaact tatgcgatta acaaaaatac tctgaaatga caagtaatca tttcagagca
     6721 cgcaaaaagc acaaatatac ttttatcttc tttcatccag actatactgt cggctttgga
     6781 atttcaccaa atcatgcctt tcggctcgtg ggctatacca ccggtaggga attacaccct
     6841 gccctgaaga tagttattca attacagatg attatagtac ttaattttga atatgtcaac
     6901 agataaatac cgattgtttt tgatatactg tatttgtgat aatcgattct cgctcctcgg
     6961 ataaagaaaa tatgatatac tagataaacg aaataagaga gaaggaatac tatgtacgca
     7021 tatttaaaag gaatcattac caaaattact gccaaataca ttgttcttga aaccaatggt
     7081 attggttata tcctgcatgt ggccaatcct tatgcctatt caggtcaggt taatcaggag
     7141 gctcagattt atgtgcatca ggttgtgcgt gaggacgccc atttgcttta tggatttcgc
     7201 tcagaggatg agaaaaagct ctttcttagt ctgatttcgg tctctgggat tggtcctgta
     7261 tcagctcttg ctattatcgc tgctgatgac aatgctggct tggttcaagc cattgaaacc
     7321 aagaacatca cctacttgac caagttccct aaaattggca agaaaacagc ccagcagatg
     7381 gtgctggact tggaaggcaa ggtagtagtt gcaggagatg gccttcctgc caaggtcgca
     7441 gtgcaagcaa gtgctgaaaa ccaagaattg gaagaagcta tggaagccat gttggctctg
     7501 ggctacaagg caacagagct caagaaaatc aagaaattct ttgaaggaac gacagataca
     7561 gctgagaact atatcaagtc ggcccttaaa atgttggtca aataggagca gagaatgaca
     7621 aaacgttgtt cgtgggtcaa gatgaccaac ccgctctaca tcgcctatca tgatgaggag
     7681 tggggtcagc ccctccatga tgaccaagca ttgtttgagt tgttgtgtat ggaaacctat
     7741 caggcaggcc tgtcttggga aacggtactc aacaaacgcc aagctttccg agaagccttt
     7801 catagctatc aaattcactc agtcgcagag atgactgaca ctgaattgga agccatgctg
     7861 gagaatccag ctatcattcg aaatagagcc aagatttttg ctacacgcgc taacgcccaa
     7921 gcctttctac agttacaggc agagtacggc tcttttgatg cctatctttg gtcttttgtt
     7981 gaggggaaaa ctgtcgttaa cgatgttcct gattatcgcc aagccccagc taaaacaccc
     8041 ttatctgaga aattagccaa agatctcaaa aaacgaggct tcaagttcac aggcccagtc
     8101 gccgtattgt cttttctaca ggctgcaggg ctagttgatg accacgagaa tgattgtgag
     8161 tggaaaggtc ttaaatgatg tctaacaaaa ataagggaat tctgattttt gcgattctct
     8221 atacagtcct ctttgtgttt gatggcgtta aattgctggc ttctttaatg ccatctgcca
     8281 ttgcaactta tcttgtttat gtagttttag ctttatatgg ctccttcttg ttcaaggata
     8341 gattgatcca acaatggaag gagattagaa agactaaaag aaaattcttc tttggagtct
     8401 taacaggatg gctctttctc attctgatga ctgttgtctt tgaatttgta tcagagatgt
     8461 tgaagcagtt tgtgggacta gatggacaag gtctaaatca gtctaatatt caaagtacct
     8521 ttcaagaaca accactactg atagctgttt ttgcttgtgt cattggacct ctggtagaag
     8581 aattattttt ccgtcaggtc ttattgcatt acttgcagga acggttgcca ggtttactaa
     8641 gcattattct ggtaggactt gtttttgctc tgactcatat gcacagtttg gctctatcag
     8701 agtggattgg tgcagttgat tacttaggtg gaggccttgc cttttctatt atttatgtga
     8761 aagaaaaaga gaatatctac tatcccctac ttgttcacat gttaagcaac agcctctcct
     8821 taatcatttt agctatcagt atagtaaaat gaaataagaa caggacaaat caatcaggac
     8881 agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta gtttcaatac
     8941 actgtaacat gtgatgaaaa tgccagtaat gataccgaga aaaaagctga gaaacttttc
     9001 ccagctttat ttgttatagt caaagcgaat gacttgttcc tgtgcatcta catgagcatg
     9061 gaccccaaag ggtacaattg ctcttggagt tgcgtggccg acattcagat tatagacaat
     9121 cgggatattg ttgtcaatga tatccaatag tgcctcttta tagtcgtcat ggaaagtttc
     9181 atccataggt tttccgacca agagtccatt gatgaccgcg aatatgccag tgtcctttaa
     9241 agttagcaac atctttttga agtcttctgg cttaggctct tcttcgcttg tttcgagcaa
     9301 gaggattttc ccttcccagt ctgacaagtc agggaaaagt ttgtattttt ggcagagttc
     9361 cgtgctatct gcgtatcgag agttgtcaaa gatatcgtag agggattcga ggcaaccacc
     9421 gaggattttc ccctcgaact gagcacttcc ttgcaacaag tcaaaacctg tatttgtatg
     9481 actgacacga ggtgttccca gggccgtggg actaaaatca gttcgttcct cataccaaac
     9541 gtcactaggg cggatttctg aaattcttcc cgtctcaatc aattctttaa agtagtgaag
     9601 gctataggct agcatttctt tgtctaattc acaaatgtct gctaaaaagg attgaccata
     9661 aaaagtcttg attcctaatt tatgcaacat gaggtggttc atggttgtat ccgagaagcc
     9721 aagaaaaatt ttttgtttga taaccttttg gagttggtca ttttcaaaaa gataaggtag
     9781 caagcgatag gtatcgtctc cacctatggc acataggatc atgtcgatgc tatcatcaga
     9841 aaaggcatga atcaaatcct ctgcacgagc ttcaggatgg tccttgataa agtctaatcc
     9901 ttttaacgaa tggggcaaaa agatgggatt gagtcccaga accttgagac gttggatacc
     9961 caagtccact tcgtgtttga caaaatcctc tccgataatg ccactagata aactaacaat
    10021 accaatagta gaaaccatat ctcatcctcc tagaaataga ttgagcctat tttatcacaa
    10081 aatataagag aaagctatgt gggatttcag aagaatactt ttaatactct tcgaaaatct
    10141 cttcaaacca cgtcagtttt atctgcaacc tcaaagctgt gctttgagca acctgcagct
    10201 agcttcctag tttgctcttt gattttcatt gagtataact caaaaaagta agtgaaaaag
    10261 caaccgcacc tgcagttgct tttatcgttt tcttattctt gatagcgtgt tggtcctgca
    10321 cttgcactac ggatattcaa gcgtttcttg agatagaagc gaagggctag gagagctgct
    10381 ccaataatag ctagtggcaa tggatccagt actgggttaa ggctagctgg taggaagctt
    10441 gttgcaaaga agacaagcaa ccaaaggaac atagaagcta ggataactag tacagatttc
    10501 cagaaaggtg gacgttgact gcgatccata tctggtccat agtattggta aacaaagtag
    10561 tacatcaagt agaaggcaaa tccaccaacc agtccaacta atagaagagt aatcaatcca
    10621 tagccgaaag cttgatctgc cgcaaagaag gttgtgaggg cgctgacaag ggcaaagagg
    10681 ctagtgatga aaagagctga gtccataatc atcagttttg ggtcatcatt ttcttttgga
    10741 tgctcttttt cgtactgctc tttgacagtg aagctatgag cccaatgagt tggtgcgccg
    10801 tatagggaac gggcagttgt acctttagat tgctcctcaa ggatttgggg aatgacttcc
    10861 tcaaaaatag tctggatttc agcgtctgtt ttcccatctt tgatgaattg ttgggtagca
    10921 atgtggacaa actcttggtt tttcttagtt aatttttgta gatcaatttg agacatagga
    10981 actcctctta gaaccatttt ttatggatga gatagagagt gagcgagaca ctcatagcaa
    11041 aggcgataaa gacgattaac cagaaggcat ttggctctcc gtttagggga atttcattat
    11101 ccttaaagtt catcccgtag gcagaaaaga ccatggttgg gatggacatg acgatggtca
    11161 caagggccaa ggttttcatg atgttgttct ggttgttaga aatgatagag gcaaaggtct
    11221 ctgtcataga atgcaagacg tttccataaa tatctgccat ctcgatggcc tgttgggttt
    11281 caatcagggt gtcttcaagc aggtcttcgt cctcaaggta tttcttgata ttgctggttg
    11341 aactggtcaa tttcttaatc acgcgctcat ttgttttgag ggaggccttg aaatagacga
    11401 tagttttttc caattccatg agctcaatca attcttcatt acgagttgat tgatgcagtt
    11461 gactttcgat ttgttcactc ttgcggtcga ttgaacgaag ggctgttagg taaagctctg
    11521 cattgcgata aagaatttga aagataaaac gtgaacgcat gaaggtatag aaattacgca
    11581 atcgacggtt gataaagaca tcaaggacag gtagtggttc caaacacgta gtgataatgg
    11641 tttcctcagt gatgataata ccaagcggga tggttacgta gtaggtgcgg ttatttcttt
    11701 cctccgtgac cggcacgtct acgataatca gggtatactc gtcttcaatg gtaatacgag
    11761 acatttcttc cgcatcgagc ggtgctcgaa ggtcggcaat atcaatatcg aaggtgttgg
    11821 cgatttcgag tgattcattt tgagtcggat tgacgagatt gatccaagta cccggttcaa
    11881 gcgtatcgat ctctttaaat tcagttgttg tagagagaaa aacttgtttc atatccctta
    11941 tcctttctca tttttcagat tttttcacac tgtactatta tactacaaaa tcggcctttt
    12001 gggtaataga atctcacttt ttttggtata atggtaagca ataatggact agaaagaaca
    12061 aagatgcaag ataaaattgt cattcatggg gcgcgtgccc ataatttaaa aaatattgat
    12121 gtggagattc cgcgagacaa gttggttgtc gtgactggct tgtcaggttc agggaagtcc
    12181 agtctggctt ttgatactct ctatgcggag ggacaacgtc gctatgtaga gagtttgtct
    12241 gcctacgctc gtcagttctt gggaaatatg gaaaaaccag atgtagatgc cattgatggt
    12301 ctcagcccag ctatttccat cgaccagaaa acgactagta aaaatcctcg ctcgacggtg
    12361 ggaacgacga ctgaaatcaa tgactatctg cgtctcctct acgcacgtgt ggggacgcct
    12421 tactgtatca acggacatgg agctatcaat gcttcttctg tggagcaaat cgttgataag
    12481 gttttggagt tacctgaacg ccagcgcttg cagatcttgg ctcctgtcat ccgcaagaaa
    12541 aaaggccaac ataagagtgt tatcgagaag gttcagaaag acgggtatgt tcgtgttcgt
    12601 gtggatgggg aagtctatga tgtgaccgaa gtgccagagt tgtctaagag caagcaacac
    12661 aatatcgatg tcgtggttga ccgtattgtt atcaaggagg gcattcgtag ccgtctcttt
    12721 gattccattg aagctgccct tcgtatcgca gaaggttatg tgattatcga caccatggac
    12781 gactctgagt tgttgttctc tgagcattat gcctgtccag tttgtggatt tactgtccca
    12841 gagttagaac ctcgtctctt ctctttcaat gctccctttg gctcttgtag tgagtgtgac
    12901 ggcttgggga tcaagctgga ggtagatact gatttggtag tgccagatgc cagcaaaacc
    12961 ttacgtgagg gagcgctagc accttggaat cctatctcat ccaactacta tccaaacatg
    13021 ttagagcagg ccatgaaagt ctttggagtg gctatggata agccttttga ggacctgtca
    13081 gaagaagata agaacttgat tctctatggg tcagatggta aggaattcca tttccactat
    13141 gagaatgaat ttggtggcgt gcgcgatatc gacattccgt ttgagggagt tatcaataat
    13201 atcaaacgtc gttaccatga aaccaatagc gattacactc gcactcagat gcgtctctac
    13261 atgaatgagc tgacctgcgg aacctgtcaa ggctatcgtc tcaatgacca ggccttgtct
    13321 gtccgtgtgg gcggccagca agggccacat attggagaaa tctcagacct gtctatcgct
    13381 gaccacttgg acttggtgag ccagttaact ttgtctgaaa atgaagccat cattgctcgt
    13441 cccattctca aggaaatcaa ggatcgtttg accttcctta ataatgtggg tcttaactat
    13501 ctaaccctgt cacgttcagc aggaaccctt tcaggtgggg aaagtcagcg cattcgtttg
    13561 gcaacccaga ttggttccaa cctatcaggc gtcctctata tcctagacga gccgtcaatc
    13621 ggtcttcacc agagggacaa tgaccgcctg attgccagtc tgaaaaagat gcgtgacttg
    13681 ggcaatactc ttatcgtggt ggaacacgac gaagatacca tgcgtgaagc tgattatctg
    13741 attgacgttg gtcctggtgc cggtgttttt ggtggtgaga ttgttgcagc aggtacacct
    13801 aagcaagtgg ctcgcaacag caagtctatc acaggtcagt acttgtcagg caaacgtgtc
    13861 atcccagtac cagaagagcg ccgtgtcgga aatggtcgtt ttattgaagt gacaggagcg
    13921 cgtgagaaca acttgcaaaa tgtcacggct cgctttccac taggaaaatt tatcgcagtg
    13981 acaggtgtgt cgggttcagg gaaatcgact ctaatcaaca gcattctcaa aaaagccatt
    14041 gcccagaagc tcaaccgtaa ttcagacaaa cctggtaagt ttaagactat tacagggatt
    14101 gagcatgtcg accgtttgat tgatattgac cagagcccta tcggacggac gccgaggtct
    14161 aacccagcta cctatacagg agtttttgac gatatacgtg acctttttgc tcagacaaat
    14221 gaagccaaga ttcgaggcta caagaagggg cgcttcagtt tcaacgtcaa gggaggtcgc
    14281 tgtgaagctt gctcaggtga cgggattatc aagattgaga tgcacttctt gccagatgtt
    14341 tatgtggctt gtgaagtttg ccacggaacc cgctataaca gtgaaaccct agaagttcac
    14401 tacaaggaaa agaatatttc gcaggtcttg gatatgacgg tcaacgatgc ggtagaattt
    14461 ttccaacaca ttccgaaaat tcaacgcaaa ctccagacca tcaaggatgt agggctaggc
    14521 tatgtgacct tgggacagcc agctaccacc ctttctgggg gagaagccca acgtatgaag
    14581 ttagctagcg aactccacaa acgctcgaca ggaaaatctt tctacattct ggatgagccg
    14641 acgacagggc ttcatacaga ggacattgct cgcctgctca aggttttagc tcgctttgta
    14701 gacgatggca atacagtcct cgtcatcgag cacaatctgg atgttatcaa gacggcagac
    14761 catatcattg acttgggacc tgagggcggt gtcggtggtg gaaccatcat cgtaacagga
    14821 actccagaag aagtagctgc caacgaagcc agctatacag gacactattt gaaaggaaag
    14881 ttacatcatg aataaacgcg tacaagcatt tctagctaaa atgcaagaaa aagaactaga
    14941 tggtatcatc atcaacaatc ttaaaaacgt ctattatttg actggttttt ggggctcaaa
    15001 cggaacagtc tttatcagtc gtgaccgtca ggtcttagtg acagactctc gctatatcat
    15061 cgcagctaag caagaaacca gtggttttga gattgtggct gatcgtgatg aattggctgt
    15121 cattgcagga attgttaagg acatgggctt gactcgtatc ggttttgaag atgagatttc
    15181 agtgtcttat taccaccgta tgcaggcagc ttttgcaggt ttggacttgt ttccgcaaac
    15241 tcagtttgtg gaaggtcttc gtatgattaa ggatgaggca gagattgcag cgattcgcaa
    15301 ggcttgttct atctcagacc aagctttccg cgatgcgctt gactttatca aaccaggaaa
    15361 aactgaaatt gagattgcca acttccttga cttccgcatg cgtgagttgg gagcatctgg
    15421 cttatctttt gatacgatcc tagctagcgg tatcaattct tctaaacccc atgcccatcc
    15481 aatgcacaaa ccagtggagt tgggagaagc cattaccatg gacttcggct gtctctatga
    15541 ccactatgtc agtgatatga cccggactat ctatctaggg catgttagcg atgagcaggc
    15601 agagatttac aatacggttc taaaagctaa ccaagccttg attgaccaag ctaaggcagg
    15661 cttaggtttc cgtgactttg acaaaatccc tcgtgatatt atcattgagg caggttatgg
    15721 cgactacttt actcacggca ttggccacgg tattggtctg gatatccatg aggaacccta
    15781 ctttagccag acttctacag aaactattaa gacaggtatg gccttgaccg atgaaccagg
    15841 tatctatatc gaaggcaaat atggcgttcg tatcgaggat gatatcctga ttacagagac
    15901 aggttgtgaa ttattgaccc tagctccaaa agagttgata gtcatttagt tgtttgtcaa
    15961 gaaaaaagta cgaaaatgac gaaaaaagtc aaaaaaaatt aaaaataggt cgcaagtcgg
    16021 atgtttttta tggtataata gactaaactg atagtaacat gtagcgaaag gggtaggtac
    16081 atgattaaaa tttatacagt ctcaagttgt actagctgta aaaaagcaaa aacctggctc
    16141 aatgcccacc agttaagtta taaagaacaa aaccttggta aagaaggaat tacgagagaa
    16201 gaattactgg atattctaac caaaacagat aacggaatag ccagcatcgt ttcgtctaaa
    16261 aatcgctatg ccaaagccct tggagtggat attgaagatt tgagtgtcaa tgaagttctc
    16321 aatctgatta tggaaacacc gagaatttta aagagcccaa tccttgtaga tgaaaaacgc
    16381 ctgcaagttg gctacaagga agacgatatt cgtgccttcc taccacgctc tgtccgtaat
    16441 gtagaaaatg cagaagcacg tttacgtgca gctctataaa catcaaggct gggagcaatt
    16501 cccagtctta ttctatttta atttcaaaaa gaaagaagaa agaaatgaaa aaaatagttc
    16561 ttgttagtct agctttcctt tttgtcctgg ttggttgcgg acagaaaaaa gaaactggac
    16621 cagctacaaa aacagaaaag gatacgcttc agtcggcatt gccagttatt gaaaatgccg
    16681 agaagaatac agttgtaacc aagactttgg tcttgcccaa gtcagatgat ggtagccagc
    16741 aaactcaaac cattacatac aaggacaaga cttttttgag cttaacgatt caacaaaaac
    16801 gtccagtttc tgatgagttg aagacttata ttgaccaaca tggagtggaa gaaactcaaa
    16861 aagctcttct tgaagcggag gagaaggata agtctatcat tgaagctcgt aaattggtag
    16921 gtttcaaact tgaaacaaaa ctattgagcg caacggaact tcaaacaacg actagttttg
    16981 attttcaagt tctggatgtc aagaaggctt cccagttgga acatctgaag aatattggtt
    17041 tggaaaatct tttgaaaaat gaaccaagca aatatatttc agacagattg gcaaatggtg
    17101 cgacagaaca gtagaaaatc caaataaata gactgcctga attgtagaag gtaaggaatt
    17161 atgctataat ggtactattc taaggaaaga gggtgttaga atgggattta ctgaagaaac
    17221 agtacgtttt aaattggacg attccaataa aaaagaaatt agcgaaactt tgacagatgt
    17281 ttatgcttcg ttgaacgata agggttacaa cccaattaac caaatcgtag gttacgtatt
    17341 gagtggagac cctgcctacg ttcctcgtta taataatgca cgaaatcaaa tccgtaagta
    17401 tgagcgtgat gaaatcgttg aggaattggt tcgctactac ctcaaaggac aaggagtcga
    17461 tctataacct atgagaatta tgggattgga cgtcggttca aaaacggtag gggtggcgat
    17521 tagcgatccg cttggtttta cagctcaagg gcttgaaatc atccagataa atgaagaaca
    17581 aggccaattt ggttttgacc gcgttaagga attggttgat acttacaagg tggaacgatt
    17641 tgtagtgggc ttgcctaaaa acatgaacaa tacaagtgga ccgcgcgtag aagctagtca
    17701 agcctacgga gcaaagctag aagagttttt tggtttacca gtagactatc aggatgaacg
    17761 cttgacaaca gtggctgctg agcgcatgtt gattgaacaa gcagatatca gtcgcaataa
    17821 gcgcaagaaa gtcattgata agttagcagc tcagctgatt ttacaaaatt atttagatag
    17881 aaaattttaa tataaaggag aggctatgtc acacgatcat aaccacgacc acgaagaacg
    17941 tgaactaatc acactagtag atgagcaagg aaatgaaacc ttgtttgaaa tccttttgac
    18001 aattgatgga aaagaagaat ttggtaaaaa ctatgttctt ctagtaccag ttaacgcaga
    18061 agaagacgaa gacggacaag ttgaaatcca agcttactca ttcatcgaaa acgaagatgg
    18121 aacagaaggc gaattgcaac caatcccaga agactcagaa gacgaatgga acatgattga
    18181 agaagtcttc aacagcttta tggaggagtg atacagtctg ggagactgta tcagcccaac
    18241 tcccagaaat ggggaagagt tggaacgtcc agtggatgtt tttagcccag ttcctttaaa
    18301 taagagagaa ctggtaggtt cggggggacg tttttagccc agctccttta aataagatag
    18361 agctggtaag tctgggagac tgtatcagcc caactcccag aaatggggaa gagttggaac
    18421 gtccagtgaa catttttacc ccaactctta gaaattgaga agagttggaa catctagtgg
    18481 atgtttttag cccatattca cattcatagt ggctagttat tagctaacca ggtaagaggt
    18541 cgggacgaaa atcctggcct tgtttttatt agtaaccata ctttaatgcg ataattgatt
    18601 ggacttttgt caaggagatg tgtatgtttg aagtagaaga atggctccat agtcggattg
    18661 gtttaaattt tcgatcaggt ttgggccgaa tgcagcaagc ggtggatttg ttaggaaatc
    18721 ctgagcagtc ttaccctatt atccacgtaa cagggactaa tgggaaagga tctaccattg
    18781 cttttatgag ggaattattt atggggcatg gcaaaaaagt tgcgaccttt acctcccctc
    18841 atatcgtctc tatcaatgac cgaatctgca ttaatgggca acctatagca gacgcagact
    18901 ttatccgttt ggctgatcag gtcaaggaga tggagaaaac gcttctgcaa actcctgacc
    18961 agttgtcctt ttttgaattg ctgaccttgg ttgcttttct ttattttagg gagcaagagg
    19021 tggatttggt tttattagaa gtgggaattg gtggcttact tgacacgacc aatgtggtaa
    19081 ctggagagct tgctgtcatc acctccattg ggcttgacca tcaagaaacc ttgggtgata
    19141 gtctagaagc aattgcagag cagaaagctg gtattttcaa ggctggtaag aaggcagtga
    19201 ttgcgaaatt gcctccagaa gctaggcttg tctgtcagaa aaaagccgaa tctttagctg
    19261 ttaaccttta tcaggcaggt caagattttt taatgctgaa tggtgatttt tcaagctctt
    19321 tactaaatct ttcacagctg aacataggct tagaaggagt ctatcagcag gagaatgcag
    19381 ccttggcgtt gcaaactttt cttcttttta tgggagaagg aaaggaagct gttgatgaac
    19441 aggctgtaag aaaggccttg gaacagaccc attgggctgg tcgcttggag cgtattcgcc
    19501 cacagattta cttggatggt gctcataacc tccctgcctt gactcgcttg gttgagttta
    19561 tcaaagaaaa agagcaggaa ggctatcgac ctcaaatcct ttttggagcc ttgaaacgga
    19621 aggattatca agggatgtta ggttacctga ctgaaaaatt gcctcaggtg gaactcaagg
    19681 tgaccggctt tgactatcag ggggctttgg acgaaaggga tgtgacaggt tacgatatag
    19741 tttcttcttg ccgagaattt atcagcgatt ttgaagaaag agcagatgct caggatttgc
    19801 tgttcgttac agggtctctc tattttatct cagaagtacg gggctacctg ctggaccgtg
    19861 agcagataaa ttgaccccct tgtttgaact tgttatacta ggggaattgc tggttagaga
    19921 aaatttctct aaattggtag cagaaaggaa attcatcatg aaattaaaaa gattcacact
    19981 ttctcttgct tctctagcaa gttttagtct cttagtagct tgttcacaaa gagctcaaca
    20041 ggttcaacag cctgttgctc agcagcaggt ccaacagcct gctcaacaac ctgctcaaca
    20101 gaataccaat actgcaaatg caggaggtaa ccaaaatcaa gcggctccag tacaaaacca
    20161 acctgttgct caaccgaccg atattgatgg gacttatact ggtcaggatg acggagaccg
    20221 tatcacttta gtggtaactg gaacgactgg tacatggact gagctcgaat ctgacgggga
    20281 tcagaaagtc aaacaggtta catttgattc agcaaatcaa cgcatgatta ttggcgatga
    20341 tgtcaaaatt tacactgtaa acggtaatca aatcgtcgta gatgatatgg atagagaccc
    20401 atcggaccaa atcgttttaa ctaaataaga ctatgcaagt agaaagggct ggatttttca
    20461 gcctttttac ttttacctag aaaataatca taaatacttg ccttctaccg aatacctgcg
    20521 ttatactagt atcaattact tgtttttagc attcaaacta tcaaggaggg gatatgaaat
    20581 ataggaaatt tcaattattg atgtccaagt atggctttag tctttcgatt atgttacttg
    20641 aactttgtct tgtttttggt ctctttcttt atttaggacg catggctccc attttatgga
    20701 ttactgtcct cattctactg agtatcatca caatcatttc gatagtcaac cgtaatacga
    20761 ctcctgagaa taaggtaacc tggttgttag tagcctttgt gccagtattt ggtcccttgc
    20821 tctatctgat gtttggtgaa aggcgattgt ccaaaaaaga aatcaaacaa ctgaagaagc
    20881 taggctctat gcatttccaa gaagcaaata gccagctact aaaagagaaa ttaaaagaaa
    20941 gtgacaaggc agcttatgga gtcatcaagt ccttattgag tatggatacc aatgctgaca
    21001 tctatgatca aactgcctct acattttttc ctaacggaga agctatgtgg aaaaagatgg
    21061 tagaagatct taaaaaggct gagaaattta ttttcttgga atattacatt atagaagaag
    21121 gtttgatgtg gaatcgcata ctagatatac tagagcaaaa ggtagctcag ggtgtagagg
    21181 ttaagatgct ctatgatgat atcggctgta tggctacttt aacaggagat tatgcacatc
    21241 gacttcgtca gctgggcatc gaggcccata aattcaataa agttattcct cgtttgacag
    21301 tggcttataa taacagagat catagaaaaa tattgattgt tgatggtcag atagcctata
    21361 ctggtgggat caatctggca gatgagtaca ttaaccacgt cgagagattt ggttattgga
    21421 aggatagtgg aattcgctta gacggactag cagtaaaagc tctgacacgc ttatttttga
    21481 ccacttggta cattaatcga ggagaaatta gtgattttga tcaatatcat ttagaaaatc
    21541 attctatccc gagtgacggt ttaaccattc catacggaag tggacccaag ccaatttttc
    21601 gagcgcaggt agggaaaaaa gtttatcaga gtttaatcaa tcaagcaaca gaatcggtct
    21661 atattacgac accttatttg attatagatt atgatttaac agagacaatc aaaaatgcag
    21721 ctatgagagg ggtcgatgtt cgaattatca ccccttacat accagataag aagttcattc
    21781 agttagtcac gagaggagct tatcccgacc ttctttctgc tggtgttcgg atttatgagt
    21841 atagtccagg ttttattcat agtaagcaga tgttggtaga cgaagatttt gcggtggtgg
    21901 ggacaatcaa tctcgactac cggagcttgg tacaccatta tgaaaatgca gtcttactct
    21961 ataaaactcc ttctataagg gaaatcgccc gagattttcg aaatatattt gcagattctc
    22021 aggaagtcta tcctcattct atcaaaacga gctggtatca aaagcttgta aaagaaatcg
    22081 cccagctatt cgcccctatc ttataagaaa tctagaactt aggatacaaa ccagacctat
    22141 ttttcttgtc ttttacgaat agaaagaaag aggaggggaa ttcatgctaa ctaaggaaga
    22201 agtgaatgat atgattgagt tcaaacagac acatttacac cgctttcggg aaattgaaac
    22261 ctttgtgaaa ctatgcaaaa aatcactcaa acagccatcg caagctgaca atcctaggac
    22321 tttttgatat actaagacag ataaattgaa caggagaaac gatatgagtg tgtatggtag
    22381 agtagaagaa gttcataagg agaatcgtga acctctagaa taccaaatcg aacaagaatc
    22441 gcatcatcgt gaatcaagtc gtcttccttt ggtgaaaatt ttactatgga gtacgcttgt
    22501 aacggggata actctaggag taccgctatt actcgactta atgagtgcac aagaagtgca
    22561 ggatttttat gcaggttggg cgcttcatca gacagggaag atttacagcg actattatgg
    22621 aagtcaaggt ttgctttatt atttgctgac ttacgtgagt cagggcggat ttttctttgc
    22681 catctttgag tggttagcct tggtagcagg aggatttttc ctttttagat cagcggacac
    22741 cttgacagag caaggagacc aagctggaca tctggtgact attttttaca tgctagttac
    22801 aggtcttgct tttggtggag gctatgcgac tcttttagcg cttcctttct tattcgcagc
    22861 ctttagttta gttgcggctt acctaagcaa tccaagccat gataagggat ttgtacggat
    22921 tgggctagct ttggcaggcg gatttttctt tgctccctta tcatcgctcc tgtttattgc
    22981 tgtagtgagt ttaggcttgt tggtctttaa ccttgggcat agacgctttg cgcatgggtt
    23041 ttatcagttt cttgcagtgg ctttaggttt ttcacttgtc ttttatccaa ctgcctacta
    23101 tagtgctgca acaggaagtt ttggggatgc gattagtggt attcgttatc ctattgacag
    23161 tattcgcttt gattttactt ctaaaatttt agagaatatg tttttttatg gcttgttgtc
    23221 ccttggtttg ggatttgtgg tttgtatctt tttaggtctc tttcaatcca aaccctttaa
    23281 actatacgtc atttcggttc ctgcaagtct tgtggtaatt ttaggtttga ttttactttt
    23341 cttttcacaa gagcctctgc acgcttctta tttgatggtc gtcttccctg ttttcctact
    23401 tttattggta accaatatta agagtcaaca gagggggcgt agtgctagaa gaagccgaag
    23461 agaaacgcca gttagcctat ggagtcgttt cttcaaagga aatctatatc tgctagtttt
    23521 tgagtttgtc tatcttttgt ctgttccttt tttgatgaag tttgtccttt atccagtacc
    23581 ttatcaagaa cgtaatcgtc ttgctgattt ggtaaaagag gagacaaata cggaagatgc
    23641 tatctatgca tgggatgata ctgcgactct ttatcgtaag agtgagcgct tgtcgccatc
    23701 ggcgattttg tccccgttgc actatacagc aactgaggaa aatcgtaata agttacttaa
    23761 tgacttgaaa gaaaaacaac ctaaggtgat tgtggtaaat gataaggtgg tagtctggtc
    23821 tgaagtggaa acactcttaa aagaaaatta ccaacaagta aagactgatt actcagagtt
    23881 taaagtctat aaaattaaat aaccaaatca atatcttgtg tatttttaaa aattttagga
    23941 tttttaacac aagatattga tttttctttt tagagtggta taatactttt tagaaagaac
    24001 attttagaaa agagcatgca tatgattgca ctagaagaaa aaattacaat tttgccaact
    24061 ctcttcgtcg agaaacgaga tgggagacgt gttgtatttg atgtggacaa gattgacaag
    24121 gctctccaca aggcggctga caaggttatg gatgtgacac ccctggttga aaaacgcctc
    24181 aatgatctga ctgagcgaat tattacagaa attcatagtc gctttccaca gggaattaag
    24241 atttacgaaa ttcaaaatat cgtagaacat gaactccttg aagccaaaga atatgcgctg
    24301 gctgaggagt atattactta tcggacacag agggattttg agcgctcaaa agcgacggat
    24361 atcaacttta gtattcataa acttctcaac aaagaccaga cagttgtcaa tgaaaacgct
    24421 aataaagaca gtgatgtctt taacactcag cgtgatttga cagcagggat tgttgggaaa
    24481 tcaatcggac tgcaaatgct tcctaagcac gtagccaatg cccaccaaaa gggggatatc
    24541 cactatcacg atttggacta cagtccctat acccctatga ccaactgctg tttgattgat
    24601 tttaagggta tgttggaaaa tggttttaag attggaaatg cagaggtaga gagtcccaag
    24661 tctatccaga ctgcgacagc acagatttct caaatcattg ccaacgttgc ttctagccag
    24721 tacggtggct gttcagctga ccgtatcgat gaaattttgg cgccttatgc agagaagaat
    24781 tatcaaaaac atctcaaaga tgcagaagag tgggtattgc ctgaaaaacg ggaagattac
    24841 gcttggaaga aaacgcaaaa ggacatctac gatgccatgc aatctcttga gtatgaaatc
    24901 aatactctct tcacttcaaa tggacaaaca ccttttactt cgttaggttt tggtctggga
    24961 accagtcgtt ttgaacgaga aattcaaaaa gctattttaa acattcgcat caagggtctt
    25021 ggttcagaac accgtacggc tatctttcct aaacttattt tcactctgaa aagaggcctt
    25081 aatttagagg aaggaactcc caactacgac atcaagcagt tggctcttga gtgtgcaacc
    25141 aaacggatgt atccagatgt tttatcttac gataagattg ttgacttgac aggatccttc
    25201 aaggtgccta tgggttgccg ttctttcctc caaggatgga aggatgaaaa tggtgtagaa
    25261 gtcaattcag gtcgcatgaa tctgggtgtt gtgacggtta atctgcctcg tattgctctt
    25321 gagtctgaag gtgatatgaa taagttctgg gaaatcttca acgagcgaat gaatatcgca
    25381 gaagatgctc ttgtttaccg tgtcgaacgc actaaagagg cgacaccagc aaatgctcct
    25441 attctttatc agtacggtgc ttttggccat cgtctaggta aagaagaaag tgttgaccag
    25501 ctctttaaga atcgtcgtgc taccgtttcg ctgggctata tcggcttgta tgaagtagcg
    25561 acagttttct ttggtaacag ctgggaaagt aatccagatg ctaaggaatt cacgctagac
    25621 atcattcacg atatgaaacg ccgtgtagaa gagtggtcag accaatatgg ctaccatttc
    25681 tctatctact caacaccatc cgaaagtctg acagaccgtt tctgccgact agatatagac
    25741 aagtttggct ctattcctga tatcacagac aaggaatact acaccaactc tttccactac
    25801 gatgttcgta aaaatccaac accgtttgaa aaattggact ttgagaaagt ctatccagaa
    25861 gcaggtgcgt caggtggttt catccattat tgtgagtatc cagtccttca gcaaaatcca
    25921 aaggccttgg aagctgtctg ggattatgct tatgaccgtg taggctatct aggcaccaat
    25981 actccgattg accgttgcta caagtgtgac tttgaagggg attttgaacc aactgagaga
    26041 gggtttgctt gtccaaactg tggcaatagc gaccctaaaa cagtagatgt ggtgaagcga
    26101 acttgtggct acctaggtaa tcctcaagca agaccgatgg tcaacgggcg tcacaaggaa
    26161 atcgctgcgc gtgtcaaaca tatgaatggt tcaacgatta aaatagctgg gcatcaagta
    26221 acaaattaga aagaaatgaa atgggaaaat atcaactaga cgataagggg cgcgcacaag
    26281 tgacccgtta tcacgagaaa cactctaaag gtggagctgg taagaaagaa cgcttgctta
    26341 gcttcagaga acaattttta aacaagaaca agaaaaaata aaagtgagag ccagctctcg
    26401 cttttctcat agtgggaggt aaggatggaa ttacgcagac caagattagc ggataagaaa
    26461 gctgttttag atatgatgac agagtttgaa aagagtcaat cagcccatga tggaggattt
    26521 tgggatgttg aggatttttc ttacgaggag tggctagaag gtaaccgtaa taaagaaatg
    26581 ggaatatggc tacctgaaaa tcgcgttcca tcgatacagt tcgtattgtt tggtgaatca
    26641 ggttgtgctc taggattttt gaatctgcga ttgagactga atgaggggtt actgaattat
    26701 gctggccaca ttggctactc catccgtcca tctgaaagag gcaagggtta tgcaaaagag
    26761 actctccgtc agggcttgca agttgctaag gaaaagaaca tcaagaaagc tctggtgacc
    26821 tgtagcgtga ataatcttgc tagcagagca gtcattctag caaatggtgg aatatttgag
    26881 gatgctcgca atggagtcga gcgttattgg atagaggtag cgaatgaata atccaaaacc
    26941 acaagaatgg aaaagcgagg aacttagtca aggtcgtatc attgactaca aggcctttaa
    27001 ctttgtggac ggcgaaggcg tgcgtaactc tctctatgta tcaggctgta tgtttcactg
    27061 cgagggatgt tataatgttg cgacttggtc ttttaatgct ggcattccct atacagcaga
    27121 attagaagag cagatcatgg cagatcttgc tcagccctat gttcaaggtt tgactttgtt
    27181 gggaggggag ccttttctta atactgggat tctcttgcca cttgttaagc ggattcggaa
    27241 ggaattgcca gacaaggaca tctggtcctg gaccggctac acttgggaag aaatgatgtt
    27301 ggaaactcca gataaactgg aattcttgtc actgattgac attcttgtcg atggaagata
    27361 tgatcgaact aagagaaatc ttatgctcca gtttcgaggt tcgtctaacc aacgaattat
    27421 cgatgtgcaa aaatcgctca aaagtgggca agtagtgatt tgggacaagc tcaatgacgg
    27481 aaaagaaagc tatgaacagg tgaagagaga atgaagaaaa aagacttagt agaccaacta
    27541 gtctcagaga tcgagacggg gaaagtcagg acactgggaa tatacggtca tggagcttca
    27601 ggtaaatcaa cctttgcaca ggaattgtac caagctttag attctactac agtaaatttg
    27661 ctagagacag atccttatat cacctcagga cgccatctgg tagtacccaa ggacgcgccg
    27721 aatcaaaagg tgacagccag tctgccagtg gcgcatgaac tggagagttt gcagagagat
    27781 atccttgcct tgcaggcgag tatggatgtc ttgacaattg aagaaccttg gaaggctagt
    27841 gaggtcttgt ctggagccaa accaattttg attgtcgaag ggatgtctgt tggctttcta
    27901 cccaaggaac tctttgaaaa aaccatctgt ttctacacgg atgaggagac cgaattaaag
    27961 cgacgccttg ctagagatac gactgtgaga aatcgcgatg catcctttat attggctagc
    28021 catcagatga gacgggagca gtatctgcga tactataaag aaactgagtc taaggcggat
    28081 atcttagtgg accaatcaga agataaattt gatgtcaaga ggacttaact tatgtataga
    28141 agaaatatag aagaaaatcc ctaattttag aaagaaaaag taggaaaaca gtttcatccc
    28201 caaaaaacga aaaaacctaa caaatccctt gcaatcgcag gggatttgtg ttattctatt
    28261 atggtgctgt aaattacagc cttagttttg atgcaagagg ttgcgacacg ctcggttgca
    28321 ttgccacgca acaccgcgtc ggttttcttg tggagctagc ctattatctt aaatagacga
    28381 aaaggagaaa aagatggcaa acaaaaaaat ccgtatccgt ttgaaagctt acgaacaccg
    28441 tacgcttgac acagcggctg caaaaatcgt agaatcagct actcgtacag gtgcacaagt
    28501 tgcgggtcca atcccacttc caactgaacg tagcctctac acaatcattc gtgcgactca
    28561 caaatacaaa gactctcgcg aacaatttga aatgcgtaca cacaaacgtt tgatcgatat
    28621 cgttaaccca actcaaaaaa cagttgatgc cttgatgaaa ttggatcttc caagtggtgt
    28681 aaacgtagaa atcaaacttt aatctaaaat ataaaagagc agaggctggt gtttcaatct
    28741 aattgaacac gggctaaact cggtgtgaaa aagataaact tcctcgcgtc tgctagacac
    28801 tgtgtcagtt tcctattttc actttgagtt tgacgccctt tgtatcttag acttgagcat
    28861 aaaaaacgct cgttaaaaac tttttgaata aaaaatatag aaaaggaact attttctcat
    28921 gacaaaagga atcttaggga aaaaagtggg aatgactcaa atcttcactg aagctggcga
    28981 attgatccct gtaacagtta ttgaagcaac tccaaacgtt gttcttcaag ttaaaactgt
    29041 tgaaacagac ggatacaacg ctatccaagt tggtttcgat gacaaacgcg aagtattgag
    29101 caacaaacct gctaaaggac atgtagcgaa agctaacacg gctcctaagc gcttcattcg
    29161 tgaattcaaa aacgttgaag gcttggaagt tggtgctgaa attacagttg aaacattcgc
    29221 agctggagac gttgttgacg taactggtac ttctaaaggt aaaggtttcc aaggtgttat
    29281 caaacgccac ggacaatcac gtggaccaat ggctcacggt tctcgttacc accgtcgtcc
    29341 aggttctatg ggacctgttg cacctaaccg cgtattcaaa ggtaaaaacc ttgcaggacg
    29401 tatgggtggt gaccgcgtaa caattcaaaa tcttgaagtt gtacaagttg ttccagaaaa
    29461 gaacgttatc cttatcaaag gtaacgtacc aggtgctaag aaatctctta tcactatcaa
    29521 atcagcagtt aaagctggta aataataaag aaaggggaaa tcagtcacaa tggcaaacgt
    29581 aacattattt gaccaaactg gtaaagaagc tggccaagtt gttcttagcg atgcagtatt
    29641 tggtatcgaa ccaaatgaat cagttgtgtt tgatgtaatc atcagccaac gcgcaagcct
    29701 tcgtcaagga acacacgctg ttaaaaaccg ctctgcagta tcaggtggtg gacgcaaacc
    29761 atggcgtcaa aaaggaactg gacgtgctcg tcaaggttct atccgctcac cacaatggcg
    29821 tggtggtggt gttgtcttcg gaccaactcc acgttcatac ggctacaaac ttccacaaaa
    29881 agttcgtcgc ctagctctta aatcagttta ctctgaaaaa gttgctgaaa acaaattcgt
    29941 agctgtagac gctctttcat ttacagctcc aaaaactgct gaatttgcaa aagttcttgc
    30001 agcattgagc atcgattcta aagttcttgt tatccttgaa gaaggaaatg aattcgcagc
    30061 tctttcagct cgtaaccttc caaacgtgaa agttgcaact gctacaactg caagtgttct
    30121 tgacatcgca aatagcgaca aacttcttgt cacacaagca gctatctcta aaatcgagga
    30181 ggttcttgca taatgaattt gtatgatgtt atcaaaaaac ctgtcatcac tgaaagctca
    30241 atggctcaac ttgaagcagg gaaatatgta tttgaagttg acactcgtgc acacaaactt
    30301 ttgatcaagc aagctgttga agctgctttc gaaggtgtta aagttgccaa tgttaacaca
    30361 atcaacgtaa aaccaaaagc taaacgtgtt ggacgttaca ctggttttac taacaaaact
    30421 aaaaaagcta tcatcacact tacagctgat tctaaagcaa tcgagttgtt tgctgctgaa
    30481 gctgaataat ctaaggagga aatatcgtgg gaattcgtgt ttataaacca acaacaaacg
    30541 gtcgccgtaa tatgacttct ttggatttcg ctgaaatcac aacaagcact cctgaaaaat
    30601 cattgcttgt tgcattgaag agcaaggctg gtcgtaacaa caacggtcgt atcacagttc
    30661 gtcaccaagg tggtggacac aaacgtttct accgtttggt tgacttcaaa cgtaataaag
    30721 acaacgttga agcagttgtt aaaacaatcg agtacgatcc aaaccgttct gcaaacatcg
    30781 ctcttgtaca ctacactgac ggtgtgaaag catacatcat cgctccaaaa ggtcttgaag
    30841 taggtcaacg tatcgtttca ggtccagaag cagatatcaa agtcggaaac gctcttccac
    30901 ttgctaacat cccagttggt actttgattc acaacatcga gttgaaacca ggtcgtggtg
    30961 gtgaattggt acgtgctgct ggtgcatctg ctcaagtatt gggttctgaa ggtaaatacg
    31021 ttcttgttcg tcttcaatca ggtgaagttc gtatgattct tggaacttgc cgtgctacag
    31081 ttggtgttgt cggaaacgaa caacatggac ttgtaaacct tggtaaagca ggacgtagcc
    31141 gttggaaagg tatccgccca acagttcgtg gttctgtaat gaaccctaac gatcacccac
    31201 acggtggtgg tgaaggtaag gcaccagttg gtcgtaaagc accatctact ccatggggca
    31261 aacctgctct tggtcttaaa actcgtaaca agaaagcgaa atctgacaaa cttatcgttc
    31321 gtcgtcgcaa cgagaaataa tattaaacta gtcgcttaag caactagtaa atccgccagc
    31381 tcggtagcgc tccataggag tgcaagccgc tgtggtacaa catttaaagg agaaaatata
    31441 aaaatgggac gcagtcttaa aaaaggacct ttcgtcgatg agcatttgat gaaaaaagtt
    31501 gaagctcaag ctaacgacga aaagaaaaaa gttattaaaa cttggtcacg tcgatcaacg
    31561 atcttcccaa gtttcattgg ttacactatt gcagtttatg acggacgtaa acacgtacct
    31621 gtttacatcc aagaagacat ggtaggccac aaacttggtg aatttgcacc aactcgtact
    31681 tacaaaggtc acgctgcaga cgacaagaaa acacgtagaa aataaggaga acataaatgg
    31741 cagaaattac ttcagctaaa gcaatggctc gtacagtacg tgtttcacct cgtaaatcac
    31801 gtcttgttct tgataacatc cgtggtaaaa gcgtagccga tgcaatcgca atcttgacat
    31861 tcactccaaa caaagctgct gaaatcatct tgaaagtttt gaactcagct gtagctaacg
    31921 ctgaaaacaa ctttggtttg gataaagcta acttggtagt atctgaagca ttcgcaaacg
    31981 aaggaccaac tatgaaacgt ttccgtccac gtgcgaaagg ttcagcttca ccaatcaaca
    32041 aacgtacagc tcacatcact gtagctgttg cagaaaaata aggaggtaaa atcgtgggtc
    32101 aaaaagtaca tccaattggt atgcgtgtcg gcatcatccg tgattgggat gccaaatggt
    32161 atgctgaaaa agaatacgcg gattaccttc atgaagatct tgcaatccgt aaattcgttc
    32221 aaaaagaact tgctgacgca gcagtttcaa ctattgaaat cgaacgcgca gtaaacaaag
    32281 ttaacgtttc acttcacact gctaaaccag gtatggttat cggtaaaggt ggtgctaacg
    32341 ttgatgcact ccgtgcaaaa cttaacaaat tgactggaaa acaagtacac atcaacatca
    32401 tcgaaatcaa acaacctgat ttggatgctc accttgtagg tgaaggaatt gctcgtcaat
    32461 tggagcaacg tgttgctttc cgtcgtgcac aaaaacaagc aatccaacgt gcaatgcgtg
    32521 ctggagctaa aggaatcaaa actcaagtat caggtcgttt gaacggtgca gatatcgccc
    32581 gtgctgaagg atactctgaa ggaactgttc cgcttcacac acttcgtgca gatatcgatt
    32641 acgcttggga agaagcagat actacatatg gtaaacttgg tgttaaagta tggatctacc
    32701 gtggtgaagt tcttccagct cgtaaaaaca ctaaaggagg taaataacca atgttagtac
    32761 ctaaacgtgt taaacaccgt cgtgaattcc gtggaaaaat gcgcggtgaa gcaaaaggtg
    32821 gaaaagaagt agcattcggt gaatacggtc ttcaagctac aactagccac tggatcacta
    32881 accgccaaat cgaagctgct cgtatcgcca tgactcgtta catgaaacgt ggtggtaaag
    32941 tttggattaa aatcttccca cacaaatcat acactgctaa agctatcggt gtgcgtatgg
    33001 gatctggtaa aggggcacct gaaggttggg tagcaccagt taaacgtggt aaagtgatgt
    33061 tcgaaatcgc tggtgtatct gaagagattg cacgtgaagc gcttcgactt gctagccaca
    33121 aattgccagt taaatgtaaa ttcgtaaaac gtgaagcaga ataaggagaa ggcatgaaac
    33181 ttaatgaagt aaaagaattt gttaaagaac ttcgtggtct ttctcaagaa gaactcgcga
    33241 agcgcgaaaa cgaattgaaa aaagaattgt ttgaacttcg tttccaagct gctactggtc
    33301 aattggaaca aacagctcgc ttgaaagaag ttaaaaaaca aatcgctcgt atcaaaacag
    33361 ttcaatctga agcgaaataa tagactaggg aaggagaaat ttcaatggaa cgcaataatc
    33421 gtaaagttct tgttggacgt gttgtatctg acaaaatgga caagacaatc acagttgtag
    33481 ttgaaacaaa acgtaaccac ccagtctatg gtaaacgtat taactactct aaaaaataca
    33541 aagctcatga tgaaaacaat gttgccaaag aaggcgatat cgtacgtatc atggaaactc
    33601 gcccgctttc agctacaaaa cgtttccgtc ttgtagaagt tgttgaagaa gcggtcatca
    33661 tctaatcaaa cctgaaagga gaaaactgaa atgattcaaa cagaaactcg tttgaaagtc
    33721 gcagacaaca gcggtgctcg cgaaatcttg actatcaaag ttcttggtgg ttcaggacgt
    33781 aaatttgcaa acatcggtga tgttatcgtg gcatctgtaa aacaagctac tcctggtggt
    33841 gcggttaaaa aaggtgacgt tgttaaagca gttatcgttc gtactaaatc aggtgctcgt
    33901 cgtgctgatg gttcatacat caaatttgac gaaaacgcag cagttatcat ccgtgaagac
    33961 aaaactcctc gcggaacacg tatctttggc ccagttgcac gtgaattgcg tgaaggtggc
    34021 ttcatgaaga tcgtgtcact tgctccagaa gtactttaat ttttaggaac aaactagtcc
    34081 cctagcttca agctagggtg cccttatggg cgtaagaaaa atcaaggaga aacctaatgt
    34141 ttgtaaaaaa aggcgacaaa gttcgcgtaa tcgctggtaa agataaggga acagaagctg
    34201 ttgtccttac tgcccttcca aaagtaaaca aagttatcgt tgaaggtgtt aacattgtta
    34261 agaaacacca acgtccaact aacgagcttc ctcaaggtgg tatcatcgag aaagaagcag
    34321 ctatccacgt atcaaatgtt caagttttgg ataaaaatgg tgtagctggt cgtgttggtt
    34381 acaaatttgt agacggtaaa aaagttcgct acaacaaaaa atcaggcgaa gtgcttgatt
    34441 aatcacgaag gaaaggagaa gtataatggc aaatcgttta aaagaaaaat atcttaatga
    34501 agtagttcct gctttgacag aacaattcaa ctactcatca gtgatggctg tgcctaaagt
    34561 agataagatt gttttgaaca tgggtgttgg tgaagctgta tcaaacgcta aaagccttga
    34621 aaaagctgct gaagaattgg cacttatctc aggtcaaaaa ccacttatca ctaaagctaa
    34681 aaaatcaatc gccggcttcc gtcttcgtga aggtgttgcg atcggtgcaa aagttaccct
    34741 tcgtggtgaa cgtatgtacg aattcttgga taaattggta tcagtttcac ttccacgtgt
    34801 acgtgacttc cacggtgtcc caacaaaatc atttgatgga cgcgggaact acacacttgg
    34861 tgtgaaagaa caattaatct tcccagaaat caacttcgat gacgttgaca aaactcgtgg
    34921 tcttgacatc gttatcgtaa caactgctaa cactgacgaa gagtcacgtg cattgcttac
    34981 aggccttgga atgccttttg caaaataata taggaggtaa atctaatggc taaaaaatca
    35041 atggtagcta gagaggctaa acgccaaaaa attgttgacc gttatgctga aaaacgtgct
    35101 gcattaaagg cggcagggga ctacgaaggt ttatctaaat tacctcgcaa cgcctcaccg
    35161 actcgtttac ataatcgttg tagggttacg gggcgcccac attcagttta ccgcaaattt
    35221 ggtctgagtc gtatcgcttt tcgcgaactt gcgcataaag gtcaaattcc tggtgtaaca
    35281 aaagcatctt ggtaatttaa gatatcaaga gtgtcaaaac tccaagtaaa aataggaaac
    35341 ttgacgaaga aactaaagtt tctaggaaag tttatctttt tcacacagag tttagcccgg
    35401 gttcaattgg gcttgccaat ttgaacacga gctacagctt tggcaaaaaa gaccaatttg
    35461 ctttggagca ttgcttctgc attaaattgt ctatttttgc tcgtgctgtt acgctctttg
    35521 tatcatgtat taactagcaa gtgcaacttg caaactacta gtaagaggag aaaaacaaaa
    35581 tggttatgac tgacccaatc gcagacttcc taactcgtat tcgtaatgct aaccaagcta
    35641 aacacgaagt acttgaagta cctgcatcaa acatcaaaaa agggattgct gaaatcctta
    35701 aacgcgaagg ttttgtaaaa aacgttgaaa tcattgaaga tgacaaacaa ggcgtcatcc
    35761 gtgtatttct taaatacgga ccaaatggtg agaaagttat cactaacttg aaacgtgttt
    35821 ctaaaccagg acttcgtgtc tacaaaaaac gtgaagacct tccaaaagtt cttaacggac
    35881 ttggaattgc catcctttca acttctgaag gtttgcttac tgataaagaa gcacgccaaa
    35941 agaatgttgg tggtgaggtt atcgcttacg tttggtaaaa tcaagataca aagctcgtaa
    36001 agaacaaagc aaaattagga agttggagaa gtttgtttac aaacaagcca acttatctat
    36061 tttgcacagt tcttagagcg tgttcagttc agctcttgaa ctaaataagt atctgaaccc
    36121 cgtgaaaact ggccgttctg gcctgacaat ttaacaggag aaaataaaca tgtcacgtat
    36181 tggtaataaa gttatcgtgt tgcctgctgg tgttgaactc gctaacaatg acaacgttgt
    36241 aactgtaaaa ggacctaaag gagaacttac tcgtgagttc tcaaaagata ttgaaatccg
    36301 tgtggaaggt actgaaataa ctcttcaccg tccaaacgat tcaaaagaaa tgaaaactat
    36361 ccacggaact actcgtgccc ttttgaacaa catggttgtt ggtgtatcag aaggattcaa
    36421 gaaagaactt gaaatgcgtg gggttggtta ccgtgcacag cttcaaggat ctaaacttgt
    36481 tttggctgtt ggtaaatctc atccagacga agttgaagct ccagaaggaa ttacttttga
    36541 acttccaaac ccaacaacaa tcgttgttag cggaatttca aaagaagtag ttggtcaaac
    36601 agctgcttac gtacgtagcc ttcgttcacc agaaccatat aaaggtaaag gtatccgtta
    36661 cgttggtgaa ttcgttcgcc gtaaagaagg taaaacaggt aaataatgtt gagtggttga
    36721 tcatcaacca ccaacctatt ttccaacttt gtgcatagca cacgatttaa aactaaagag
    36781 gtgaaaactg tgatttcaaa accagataaa aacaaactcc gccaaaaacg ccaccgtcgc
    36841 gttcgcggaa aactctctgg aactgctgat cgcccacgtt tgaacgtatt ccgttctaat
    36901 acaggcatct acgctcaagt gattgatgac gtagcgggtg taacgctcgc aagtgcttca
    36961 actcttgata aagaagtttc aaaaggaact aaaactgaac aagccgttgc tgtcggtaaa
    37021 ctcgttgcag aacgtgcaaa cgctaaaggt atttcagaag tggtgttcga ccgcggtgga
    37081 tatctatatc acggacgtgt gaaagctttg gctgatgcag ctcgtgaaaa cggattgaaa
    37141 ttctaatagg aggacactag aaaatggcat ttaaagacaa tgcagttgaa ttagaagaac
    37201 gcgtagttgc tgtcaaccgt gttacaaaag ttgttaaagg tggacgtcgt cttcgtttcg
    37261 cagctcttgt tgttgttggt gaccacaatg gtcgcgtagg atttggtact ggtaaagctc
    37321 aagaagttcc agaagcaatc cgtaaagcag tagatgatgc taagaaaaac ttgatcgaag
    37381 ttcctatggt tggaacaaca atcccacacg aagttctttc agaattcggt ggagctaaag
    37441 tattgttgaa acctgctgta gaaggttctg gagttgccgc tggtggtgca gttcgtgccg
    37501 ttgtggaatt ggcaggtgtg gcagatatta catctaaatc acttggttct aacactccaa
    37561 tcaacattgt tcgtgcaact gttgaaggtt tgaaacaatt gaaacgcgct gaagaaattg
    37621 ctgcccttcg tggtatttca gtttctgatt tggcataaga aaggggataa aatggctcaa
    37681 attaaaatta ctttgactaa gtctccaatc ggacgcattc catcacaacg taaaactgtt
    37741 gtagcacttg gacttggcaa attgaacagc tctgttatta aagaagataa cgctgctatc
    37801 cgtggtatga tcacagcagt atctcactta gtaacagttg aagaagtaaa ctaatgaagt
    37861 tttaggggat gtgcactgta ccatccccta aaactagata tagtcatcta tgatgacatc
    37921 gtataggcga gttgatgggg gagacaacct tttctccctt atcggcgcta gcattttaca
    37981 aaagaggaga aaataaaaat gaaacttcat gaattgaaac ctgcagaagg ttctcgtaaa
    38041 gtacgtaacc gcgttggtcg tggtacttca tcaggtaacg gtaaaacatc tggtcgtggt
    38101 caaaaaggtc aaaaagctcg tagcggtggc ggagttcgcc ttggttttga aggtggacaa
    38161 actccattgt tccgtcgtct tccaaaacgt ggattcacta acatcaacgc taaagaatac
    38221 gcaattgtga accttgacca attgaacgtc tttgaagatg gtgctgaagt aactccagtt
    38281 gttcttatcg aagcaggaat tgttaaagct gaaaagtcag gtattaaaat tcttggtaac
    38341 ggtgagttga ctaagaaatt gactgtgaaa gcagctaaat tctctaaatc agctgaagaa
    38401 gctatcactg ctaaaggtgg ttcagtagaa gtcatctaag agaggtgacc tatgtttttt
    38461 aaattattaa gagaagctct taaagtcaag caggttcgat caaaaatttt atttacaatt
    38521 tttatcgttt tggtctttcg tatcggaact agcattacag ttcctggtgt gaatgccaat
    38581 agcttgaatg ctttaagtgg attatccttc ttaaacatgt tgagcttggt gtcggggaat
    38641 gccctaaaaa acttttcgat ttttgcccta ggagttagtc cctatatcac tgcttctatt
    38701 gttgtccaac tcttgcaaat ggatatttta cccaagtttg tagagtgggg taaacaaggg
    38761 gaagtaggtc gaagaaaatt gaatcaagct actcgttata ttgctctagt tctcgctttt
    38821 gtgcaatcta tcgggattac agctggtttt aataccttgg ctggagctca attgattaaa
    38881 actgctttaa ctccacaagt ttttctgacg attggtatca tcttaacagc tggtagtatg
    38941 attgtcactt ggttgggtga gcaaattaca gataagggat acggaaacgg tgtttccatg
    39001 attatctttg ccgggattgt ttcctcaatt ccagagatga ttcagggcat ctatgtggac
    39061 tactttgtga acgtcccaag tagccgtatc acttcatcta tcattttcgt aatcattttg
    39121 attattactg tattgttgat tatttacttt acaacttatg ttcaacaagc agaatacaaa
    39181 attccaatcc aatatactaa ggttgcacaa ggtgctccat ctagctctta ccttccgtta
    39241 aaggtaaatc ctgctggagt tatccctgtt atctttgcca gttcgattac tgcagcgcct
    39301 gcggctattc ttcagttttt gagtgccaca ggtcatgatt gggcttgggt aagggtagca
    39361 caagagatgt tggcaactac ttctccaact ggtattgcca tgtatgcttt gttgattatt
    39421 ctctttacat tcttctatac gtttgtacag attaatcctg aaaaagcagc agagagccta
    39481 caaaagagtg gtgcctatat ccatggagtt cgtcctggta aaggtacaga agaatatatg
    39541 tctaaacttc ttcgtcgtct tgcaactgtt ggttccctct tccttggtgt gatttccatt
    39601 ttaccgattg cagctaaaga tgtatttggt ctttctgatg ttgttgcctt tggtggaaca
    39661 agtctcttga tcattatctc tacaggtatc gaaggaatca agcaattgga aggttaccta
    39721 ttgaaacgta agtatgttgg tttcatggac agaacagaat aaaagtattt actgaatcag
    39781 taaatgctga gggagtggag gtttaaactc tgacatttgt aagagttgga tctcccctct
    39841 tctattttgt ttttaaatag gggtgaaaag actttttgct tctatttaaa aataaaataa
    39901 ggagatcaaa tcatgaatct tttgattatg ggcttacctg gtgcaggtaa gggaactcaa
    39961 gcagcaaaaa tcgtagaaca attccatgtt gcacatatct caacaggtga tatgttccgc
    40021 gctgcaatgg caaatcaaac tgaaatgggt gttcttgcta agtcatatat tgacaagggt
    40081 gaattggttc ctgacgaagt tacaaatgga atcgtaaaag aacgcctttc acaagatgat
    40141 attaaagaaa caggattctt attggatggt tacccacgta caattgaaca agctcatgcc
    40201 ttggacaaaa cattggctga acttggcatt gaactagaag gtgttatcaa tattgaagtg
    40261 aaccctgaca gcctcttgga acgtttgagt ggccgtatca tccaccgcgt aactggagaa
    40321 actttccaca aggtctttaa cccaccagtt gactataaag aagaagatta ctaccaacgt
    40381 gaagatgata agcctgagac agtaaaacgt cgtttggatg ttaatattgc tcaaggagaa
    40441 ccaatcattg ctcactaccg tgccaaaggt ttggttcatg acatcgaagg taatcaagat
    40501 atcaatgatg tcttctcaga tattgaaaaa gtattgacaa atttgaaata aagcgttttt
    40561 catacttgca aaaattcgct acaaatgtta tactgagata gtctgactta taattgttgt
    40621 ctctgtgtct agaggcatcg aatcgaaatt tatggaggtg cttttgcgtg gcaaaagacg
    40681 atgtgattga agttgaaggc aaagtagttg atacaatgcc gaatgcaatg tttacggttg
    40741 aacttgaaaa tggacatcag attttagcaa cagtttctgg taaaattcgt aaaaactata
    40801 ttcgtatttt agcgggagat cgtgttactg tcgaaatgag tccatatgac ttgacacgtg
    40861 gacgtatcac ttaccgcttt aaataatcga aaaacttgga gggataagaa atgaaagtaa
    40921 gaccatcggt caaaccaatt tgcgaatact gtaaagttat tcgtcgtaat ggtcgtgtta
    40981 tggtaatttg cccagcaaat ccaaaacaca aacaacgtca aggataagat agaaaggaga
    41041 aaacatggct cgtattgctg gagttgatat tccaaatgac aaacgcgtag taatctcatt
    41101 gacttatgtt tatggtatcg gacttgcaac atctaagaaa attttggctg ctgctggaat
    41161 ctcagaagat gttcgtgtac gtgatcttac atcagatcaa gaagatgcta tccgtcgtga
    41221 agtggatgca atcaaagttg aaggtgacct tcgtcgtgaa gtaaacttga acatcaaacg
    41281 tttgatggaa atcggttcat accgtggtat ccgtcaccgt cgtggacttc ctgtccgtgg
    41341 acaaaacact aaaaacaacg ctcgcactcg taaaggtaaa gctgttgcga ttgctggtaa
    41401 gaaaaaataa tataggaggt aaaagtcttg gctaaaccaa cacgtaaacg tcgtgtgaaa
    41461 aagaatatcg aatctggtat tgctcatatt cacgctacat ttaataacac tattgttatg
    41521 attactgatg tgcatggtaa tgcaattgct tggtcatcag ctggtgctct tggtttcaaa
    41581 ggttctcgta aatctacacc attcgctgct caaatggctt ctgaagctgc tgctaaatct
    41641 gcacaagaac acggtcttaa atcagttgaa gttactgtaa aaggtccagg ttctggtcgt
    41701 gagtcagcta ttcgtgcgct tgctgccgct ggtcttgaag taacagcaat tcgtgatgtg
    41761 actccagtgc cacacaatgg tgctcgtcct ccaaaacgtc gccgtgtata atcatcgcat
    41821 tacactgctt ttcgtttaag agggagtaac taaatgatcg agtttgaaaa accaaatata
    41881 acaaaaattg atgaaaataa agattatggc aagtttgtaa tcgaaccact tgaacgtggc
    41941 tacggtacaa ctcttggtaa ctctcttcgt cgtgtacttc tagcttctct accaggagca
    42001 gctgtgacat ctatcaacat tgatggtgtg ttacatgagt ttgacacagt tccaggtgtt
    42061 cgtgaagacg tgatgcaaat cattctgaac attaaaggaa ttgcagtgaa atcgtacgtt
    42121 gaagacgaaa aaatcatcga actggatgtt gaaggtcctg ctgaagtaac agctggtgac
    42181 attttgacag atagcgatat tgaaattgta aatccagatc attatctctt tacaatcggt
    42241 gaaggttctt ctctaaaagc gactatgact gttaacagtg gtcgtggata tgtacctgct
    42301 gatgaaaata aaaaggataa tgcaccagtt ggaacacttg ctgtagattc tatttataca
    42361 ccagttacaa aagtcaacta tcaagtggaa cctgctcgtg taggtagcaa tgatggtttc
    42421 gacaaattaa cccttgaaat cttgacaaat ggaacaatta ttccagaaga tgctttaggg
    42481 ctttcagcac gtattttgac agaacatctt gatttgttta caaatcttac tgagattgct
    42541 aagtcaactg aagtgatgaa agaagctgat actgaatctg acgaccgtat tttagatcgt
    42601 acgattgagg aactggactt gtctgtgcgt tcatacaact gtttaaaacg tgccggtatc
    42661 aatactgtgc atgatttgac agaaaaatct gaagcagaga tgatgaaagt acgaaatctt
    42721 ggacgcaaga gtttggaaga agtgaaactc aaactcattg atttgggtct tggattaaaa
    42781 gataaataaa ggaggaatac atggcttacc gtaaactagg acgcactagc tcacaacgta
    42841 aagcaatgct tcgcgatttg acaactgacc ttttgatcaa cgaatcaatc gtgacaactg
    42901 aagctcgtgc taaagaaatc cgtaaaactg ttgaaaaaat gattactcta ggtaaacgtg
    42961 gtgatttgca tgcacgtcgt caagcagctg ctttcgtacg taatgaaatc gcatctgaaa
    43021 actatgatga agcaactgat aagtacactt ctactacagc acttcaaaaa ttgttctcag
    43081 aaatcgcacc tcgttatgct gaacgtaacg gtggatacac tcgtatcctt aaaactgaac
    43141 cacgtcgtgg tgatgcagcg ccaatggcga tcatcgaatt agtataaaat catcaatttt
    43201 gttgagtgtt atgatgatgg agtcttgtgc tcttagtcta gctctggtct accgctagga
    43261 tttcggtcct agcgggaaca ctcatcataa gttgggatag tagacgcttg tttacgaaat
    43321 tgtttttttc ttaagaacaa cttcgtaagc aggcgttttt gagtattttc gttagaatta
    43381 tgctatacta tttgaaaaga atcctgttta atgttaaggt ttcttatttt aagaagaatt
    43441 ggagtttact tatgaaagcc attataactg ttgttggtaa agataaatct ggaattgttg
    43501 caggtgtttc tggtaaaatt gcagaattag gattgaatat tgacgatatc tctcaaactg
    43561 tattggatga atattttacg atgatggctg ttgtatctag tgatgaaaag caagatttta
    43621 cctatcttcg taatgaattt gaagcttttg ggcaaacttt gaatgtaaaa atcaatattc
    43681 agagtgcagc gattttcgaa gctatgtata atatctagga ggtcatcatg gatattagac
    43741 aagttactga aaccatcgcc atgattgagg agcaaaactt cgatattaga accattacca
    43801 tggggatttc tcttttggac tgtatcgatc caaatatcaa tcgtgctgcg gagaaaatct
    43861 atcaaaaaat tacgacaaag gcggctaatt tagtagctgt tggtgatgaa attgcggctg
    43921 agttgggaat tcctatcgtt aataagcgtg tatcggtgac acctatttct ctgattgggg
    43981 cagcgacaga tgcgacggac tacgtggttc tggcaaaagc gcttgataag gctgcgaaag
    44041 agattggtgt ggactttatt ggtggttttt ctgccttagt acaaaaaggt tatcaaaagg
    44101 gagatgagat tctcatcaat tccattcctc gcgctttggc tgagacggat aaggtctgct
    44161 cgtcagtcaa tatcggctca accaagtctg gtattaatat gacggctgtg gcagatatgg
    44221 gacgaattat caaggaaaca gcaaatcttt cagatatggg agtggccaag ttggttgtat
    44281 tcgctaatgc tgttgaggac aatccattta tggcgggtgc ctttcatggt gttggggaag
    44341 cagatgttat catcaatgtc ggagtttctg gtcctggtgt ggtgaaacgt gctttggaaa
    44401 aagttcgtgg acagagcttt gatgtagtag ccgaaacagt taagaaaact gcctttaaaa
    44461 tcactcgtat cggtcaattg gttggtcaaa tggccagtga gagactgggt gtggagtttg
    44521 gtattgtgga cttgagtttg gcaccaaccc ctgcggttgg agactctgtg gcacgtgtcc
    44581 ttgaggaaat ggggctagaa acagttggca cgcatggaac gacagctgcc ttggccctct
    44641 tgaacgacca agttaaaaag ggtggagtga tggcctgtaa ccaggtcggt ggtctatctg
    44701 gtgcctttat ccctgtttct gaggatgaag gaatgattgc tgcagtgcaa aatggctctc
    44761 ttaatttaga aaaactagaa gctatgacgg ctatctgttc tgttggattg gatatgattg
    44821 ccatcccaga agatacgcct gctgaaacta ttgcggctat gattgcggat gaagcagcaa
    44881 tcggtgttat caacatgaaa acaacagctg ttcgtatcat tcccaaagga agagaaggcg
    44941 atatgattga gtttggtggt ctattaggaa ctgcacctgt tatgaaggtt aatggggctt
    45001 cgtctgtcga cttcatctct cgcggtggac aaatcccagc accaattcat agttttaaaa
    45061 attaagaaaa taggagaaat tttaagttct attt
//
LOCUS       DAVLYR010000004        36997 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLYR010000004 DAVLYR010000000
VERSION     DAVLYR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.004"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(132..936)
                     /locus_tag="VYO63_000210"
     CDS             complement(join(132..566,568..936))
                     /locus_tag="VYO63_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1671198.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKQLANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     CRDMKLFKMSRRNIGQAGKILSDSGYQGLMKIYPQVQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKIENIFAKVKTFKMFSTIYRNHRKRFGLRMNLIAGIINHELGF"
     gene            931..1185
                     /locus_tag="VYO63_000211"
     CDS             931..1185
                     /locus_tag="VYO63_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1671199.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1480..2814
                     /gene="trmFO"
                     /locus_tag="VYO63_000212"
     CDS             1480..2814
                     /gene="trmFO"
                     /locus_tag="VYO63_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1671200.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2898..3635
                     /locus_tag="VYO63_000213"
     CDS             2898..3635
                     /locus_tag="VYO63_000213"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1671201.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3644..4201
                     /gene="frr"
                     /locus_tag="VYO63_000214"
     CDS             3644..4201
                     /gene="frr"
                     /locus_tag="VYO63_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1671202.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            4261..5115
                     /gene="cvfB"
                     /locus_tag="VYO63_000215"
     CDS             4261..5115
                     /gene="cvfB"
                     /locus_tag="VYO63_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1671203.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGCVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            5124..5339
                     /locus_tag="VYO63_000216"
     CDS             5124..5339
                     /locus_tag="VYO63_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1671204.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5425..6393
                     /locus_tag="VYO63_000217"
     CDS             5425..6393
                     /locus_tag="VYO63_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1671205.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(6371..6550)
                     /locus_tag="VYO63_000218"
     CDS             complement(6371..6550)
                     /locus_tag="VYO63_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671206.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6586..7086
                     /locus_tag="VYO63_000219"
     CDS             6586..7086
                     /locus_tag="VYO63_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671207.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            7089..7349
                     /locus_tag="VYO63_000220"
                     /pseudo
     CDS             7089..7349
                     /locus_tag="VYO63_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7716..8042)
                     /locus_tag="VYO63_000221"
     CDS             complement(7716..8042)
                     /locus_tag="VYO63_000221"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671208.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7988..8401)
                     /locus_tag="VYO63_000222"
     CDS             complement(7988..8401)
                     /locus_tag="VYO63_000222"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV1671209.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGGGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(8402..8827)
                     /locus_tag="VYO63_000223"
     CDS             complement(8402..8827)
                     /locus_tag="VYO63_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1671210.1"
                     /translation="MELSIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDVLLLFTYLHMAAAPEL
                     ADAMLAAKITGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            9075..10028
                     /locus_tag="VYO63_000224"
     CDS             9075..10028
                     /locus_tag="VYO63_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1671211.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEEGPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            10250..10819
                     /locus_tag="VYO63_000225"
     CDS             10250..10819
                     /locus_tag="VYO63_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671212.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10887..11537
                     /locus_tag="VYO63_000226"
     CDS             10887..11537
                     /locus_tag="VYO63_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1671213.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            11521..13761
                     /locus_tag="VYO63_000227"
     CDS             11521..13761
                     /locus_tag="VYO63_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1671214.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            13941..14129
                     /locus_tag="VYO63_000228"
     CDS             13941..14129
                     /locus_tag="VYO63_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671215.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGSY"
     gene            14161..14748
                     /locus_tag="VYO63_000229"
     CDS             14161..14748
                     /locus_tag="VYO63_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671216.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMTDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            14752..15927
                     /locus_tag="VYO63_000230"
     CDS             14752..15927
                     /locus_tag="VYO63_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671217.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    16101..16232
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            16248..16778
                     /gene="infC"
                     /locus_tag="VYO63_000231"
     CDS             16248..16778
                     /gene="infC"
                     /locus_tag="VYO63_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1671218.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            16811..17011
                     /gene="rpmI"
                     /locus_tag="VYO63_000232"
     CDS             16811..17011
                     /gene="rpmI"
                     /locus_tag="VYO63_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1671219.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            17063..17422
                     /gene="rplT"
                     /locus_tag="VYO63_000233"
     CDS             17063..17422
                     /gene="rplT"
                     /locus_tag="VYO63_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1671220.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            17480..17860
                     /locus_tag="VYO63_000234"
     CDS             17480..17860
                     /locus_tag="VYO63_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1671221.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            18110..18910
                     /locus_tag="VYO63_000235"
     CDS             18110..18910
                     /locus_tag="VYO63_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1671222.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            18873..19859
                     /locus_tag="VYO63_000236"
     CDS             18873..19859
                     /locus_tag="VYO63_000236"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1671223.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            19901..21802
                     /locus_tag="VYO63_000237"
     CDS             19901..21802
                     /locus_tag="VYO63_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1671224.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(21863..23518)
                     /gene="pavA"
                     /locus_tag="VYO63_000238"
     CDS             complement(21863..23518)
                     /gene="pavA"
                     /locus_tag="VYO63_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1671225.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            23623..24120
                     /gene="ybeY"
                     /locus_tag="VYO63_000239"
     CDS             23623..24120
                     /gene="ybeY"
                     /locus_tag="VYO63_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1671226.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            24101..24496
                     /locus_tag="VYO63_000240"
     CDS             24101..24496
                     /locus_tag="VYO63_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1671227.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            24513..25412
                     /gene="era"
                     /locus_tag="VYO63_000241"
     CDS             24513..25412
                     /gene="era"
                     /locus_tag="VYO63_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1671228.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            25461..26285
                     /gene="mutM"
                     /locus_tag="VYO63_000242"
     CDS             25461..26285
                     /gene="mutM"
                     /locus_tag="VYO63_000242"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1671229.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            26285..26890
                     /locus_tag="VYO63_000243"
     CDS             26285..26890
                     /locus_tag="VYO63_000243"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1671230.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            26877..28076
                     /locus_tag="VYO63_000244"
     CDS             26877..28076
                     /locus_tag="VYO63_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1671231.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            28076..28225
                     /gene="rpmG"
                     /locus_tag="VYO63_000245"
     CDS             28076..28225
                     /gene="rpmG"
                     /locus_tag="VYO63_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1671232.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            28265..28498
                     /gene="secG"
                     /locus_tag="VYO63_000246"
     CDS             28265..28498
                     /gene="secG"
                     /locus_tag="VYO63_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1671233.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KVRGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            28600..30954
                     /gene="rnr"
                     /locus_tag="VYO63_000247"
     CDS             28600..30954
                     /gene="rnr"
                     /locus_tag="VYO63_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1671234.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAANYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            30917..31384
                     /gene="smpB"
                     /locus_tag="VYO63_000248"
     CDS             30917..31384
                     /gene="smpB"
                     /locus_tag="VYO63_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1671235.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            31400..32260
                     /gene="tehB"
                     /locus_tag="VYO63_000249"
     CDS             31400..32260
                     /gene="tehB"
                     /locus_tag="VYO63_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1671236.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            32333..33286
                     /gene="coiA"
                     /locus_tag="VYO63_000250"
     CDS             32333..33286
                     /gene="coiA"
                     /locus_tag="VYO63_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1671237.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            33305..35107
                     /gene="pepF"
                     /locus_tag="VYO63_000251"
     CDS             33305..35107
                     /gene="pepF"
                     /locus_tag="VYO63_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1671238.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            35109..35822
                     /locus_tag="VYO63_000252"
     CDS             35109..35822
                     /locus_tag="VYO63_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1671239.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            35889..36830
                     /gene="prsA"
                     /locus_tag="VYO63_000253"
     CDS             35889..36830
                     /gene="prsA"
                     /locus_tag="VYO63_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1671240.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFKKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT        11185 a         6847 c         8312 g        10653 t
ORIGIN      
        1 agagagaata tagttttggt gaaaacggac ttggaacatt tcaaaatgtg tttttagctg
       61 ctgaagatat atcggattta caaatcataa tgaactcaca gcttgagaat tacattagac
      121 ttcctgcaaa actagaatcc tagttcatga ttgataatgc cagcaatcaa attcattcgt
      181 aatccgaagc gtttacgatg atttcgatag attgttgaaa acattttaaa cgtttttact
      241 ttggcaaaga tgttctcaat cttgcttctc tccttggata gcgcatggtt acaggcttta
      301 tcttcagctg ttagcggctt gagtttgctg gatttacgtg gagtttgtac ttgaggatat
      361 atcttcatga gcccttgata accactgtca gacaagattt taccagcttg tccgatattt
      421 ctgcgactca ttttgaacaa cttcatatca cgacaatagt tcacagcgat atccaaagaa
      481 acaattctcc cttgacttgt gacaatcgct tgagccttca tagcgtgaaa tttcttttta
      541 ccagaatgat tcgctaattg tttttttagg gcgattgatt tttacttccg tcgcatcaat
      601 cattaccgtg tcctcagaac tgagaggagt tcttgaaatc gtaacaccac tttgaacaag
      661 agttacttca acccattggc tccgacggat taagttgctt tcgtgaatac caaaatcagc
      721 cgcaatttgt tcataagttc gatattctcg cacatattga agagtggcca taagaaggtc
      781 ttctaggctt aatttaggtt ttcgtccacc ttttgcgtgt ttaagttgat aagctgtttt
      841 taatacagct aatatctctt caaaagtcgt gcgctgaaca ccaacaagac gcttaaatcg
      901 tgcatcagtt agttgtttac ttgcttcatc attcatagaa ctactatacc atattttgtt
      961 tcgcaggaag tctattggaa agtaagaaat attgaagctg aggctattag aagaaattgt
     1021 gagcgtggtg ctattttttc aggtaaaata aaatatcacg aagattcaca gtttaaagga
     1081 gatcactatg ttgaatgtta tgctgtttta gataatacgg ttatagcaag agatagaata
     1141 acagtcccta tcgatccgtt atgtggaaaa gattttatag agtagcatat aattgattct
     1201 taactggaat actctctatc tctttacatc aagaaaatga ctaaacaggg aagtttgcct
     1261 tcttcccttt ttttgttata ctagtagaag aaaaaaatag aaagatttgt gggagtgaaa
     1321 aagtccgggg gactttttca gcttgagcca agaaattcga gagcgaggaa agtctggggg
     1381 actttttcag cctgagccaa gaaactcgaa atctcgtaag tctgattggc tttttcaatg
     1441 tgaatcttaa cttcacactc ccaaagaggt attagtgtcg tgtctcaatc ttatatcaat
     1501 gttatcggtg ctggtttggc aggttctgaa gcagcctacc aaatcgcaga gcgtggtatt
     1561 ccagttaaac tttatgaaat gcgtggtgtc aagtcgactc ctcagcacaa aacagacaat
     1621 tttgctgagt tggtttgttc caattctttg cgtggggatg ctttgacaaa tgcagttggt
     1681 cttctcaagg aagaaatgcg tcgcttgggt tctgttatct tggaatctgc tgaggctaca
     1741 cgtgttcctg ccggtggagc ccttgcggtg gatcgtgatg gtttctctca aatggtgacc
     1801 gaaaaagtgg tcaaccaccc cttgattgaa gtggtgcgtg atgaaattac agaattgcca
     1861 acagatgtta ttacagttgt ggcgactggt cccttgacta gcgatgccct ggctgaaaag
     1921 attcatgccc ttaataatgg cgatggcttc tatttctacg atgcagcagc gcctattatc
     1981 gatgtcaaca ctatcgatat gagtaaggtc tatctcaaat ctcgttatga taagggagaa
     2041 gcagcctatc tcaatgctcc aatgaccaag caagagttta tggatttcca tgaagccttg
     2101 gtcaatgcgg aagaagcacc gctcaattct tttgaaaaag aaaagtactt tgaaggatgt
     2161 atgccaatcg aagttatggc caaacgtggc attaaaacta tgctttatgg ccctatgaag
     2221 ccagtcggtc ttgagtatcc ggacgactac acaggacctc gtgatggaga atttaaaaca
     2281 ccttatgcgg ttgtgcaact tcgtcaggat aatgcagctg gtagcctcta caatattgtt
     2341 ggtttccaga cccacctcaa atggggagaa caaaagcgtg tcttccaaat gattccgggt
     2401 cttgaaaatg cggagtttgt ccgttatggt gtcatgcatc gcaattctta catggattca
     2461 ccaaatcttc ttgagcagac ttaccgttcc aagaaacaac caaatctctt ctttgctggt
     2521 cagatgacgg gggtggaagg ctatgttgag tcggcggctt cgggcttagt tgcgggaatt
     2581 aacgcagctc gtctcttcaa ggaagaaagc gaggttattt tccccgagac gacagcgatt
     2641 ggaagcttag ctcattacat tacccatgcc gacagcaaac atttccaacc aatgaatgtc
     2701 aattttggga tcatcaagga gttggaaggc gagcgtatcc gtgataagaa ggctcgttat
     2761 gaaaaaattg cagagcgtgc ccttgccgac ttagaggaat ttttgactgt ctaatttttt
     2821 tgaaagaatt gctcatgata ctataaaaat cttagaaatt gtgataaaat aggtaggatg
     2881 aaagaaggag agtgaaaatg gcgaatccca agtataaacg tattttaatc aagttatcag
     2941 gtgaagccct tgccggtgaa cgtggcgtag ggattgatat ccaaacagtt caaacaatcg
     3001 caaaagagat tcaagaagtt catagcttag gtatcgaaat tgcccttgtt attggtggag
     3061 gaaatctttg gcgtggagaa cctgcagcag aagcaggtat ggaccgtgtt caggcagatt
     3121 acactggaat gcttgggact gttatgaatg ctcttgtgat ggcagattca ttgcaacaag
     3181 ttggggttga tacgcgtgta caaacagcta ttgctatgca acaagtggca gagccttatg
     3241 tccgtggacg tgcccttcgt caccttgaaa aaggccgtat cgttatcttt ggtgctggaa
     3301 ttggttcacc atacttctcg acagatacaa cagcggccct tcgtgcagct gaaatcgaag
     3361 cagatgccat cctcatggct aaaaatggcg tcgatggtgt gtacaatgcc gatcctaaga
     3421 aggacaagac agccgttaag tttgaagaat tgacccaccg tgatgttatc aacaaaggtc
     3481 ttcgtatcat ggactcaaca gcctcaaccc tctcaatgga caacgacatt gacttggttg
     3541 tcttcaacat gaaccaatca ggcaacatca aacgtgtcgt atttggtgaa aatatcggaa
     3601 caacagtttc aaataatatc gaagaaaagg aataagaaag aatatggcta acgtaattat
     3661 tgaaaaagct aaagagagaa tgacccaatc tcaccaatca cttgctcgtg aatttggtgg
     3721 tatccgtgct ggtcgtgcca atgcaagctt gcttgaccgt gtacatgtag aatactatgg
     3781 agtcgaaact cctcttaacc aaatcgcttc aattacgatt ccagaagcgc gtgttttgtt
     3841 ggtaacacca tttgacaagt cttcattgaa agacatcgaa cgtgccttga acgcttctga
     3901 tcttggtatc acaccggcta atgacggttc tgtgattcgc ttggttatcc cagctcttac
     3961 agaagaaact cgtcgtgacc ttgctaaaga agtgaagaag gtcggcgaaa atgctaaagt
     4021 ggctgtccgc aatatccgtc gcgatgctat ggacgaagct aagaaacaag aaaaagcaaa
     4081 agaaatcact gaagacgaat tgaagactct tgaaaaagat attcaaaaag taacagacga
     4141 tgctgttaaa cacatcgacg acatgactgc taacaaagag aaagaacttt tggaagtcta
     4201 aaaataaaca gaaaaactca gttggcattg ctggctgagt tttattcgaa agaaggaaat
     4261 atgaatacaa atcttgcaag ttttatcgtt ggactgatca tcgatgaaaa tgaccgtttt
     4321 tactttgtgc aaaaggatgg tcaaacctat gctcttgcta aggaagaggg ccaacataca
     4381 gtaggggata cggtcaaagg ttttgcatac acggatatga agcaaaaact ccgcctgaca
     4441 accttagaag tgactgccac tcaggaccaa tttggttggg gatgtgtcac agaggttcgt
     4501 aaggacttgg gtgtctttgt agatacaggc cttcctgaca aggaaatcgt tgtgtcactc
     4561 gatattctcc ctgagctcaa ggaactctgg cctaagaagg gcgaccaact ctacatccgt
     4621 cttgaagtgg ataagaaaga ccgtatctgg ggcctcttgg cttatcaaga agacttccaa
     4681 cgtcttgctc gtcctgccta caacaacatg cagaaccaaa actggccagc cattgtttac
     4741 cgtctcaagc tgtcaggaac ttttgtttac ctaccagaaa ataatatgct tggttttatt
     4801 catcctagcg agcgttacgc agagccacgt ttggggcaag tattagatgc gcgcgttatt
     4861 ggtttccgtg aagtggaccg cactctgaac ctctccctca aaccacgctc ctttgaaatg
     4921 ttggaaaacg atgctcagat gattttgact tatttggaaa gtaatggcgg tttcatgacc
     4981 ttaaatgata agtcatctcc agacgacatc aaggcaacct ttggcatttc taaaggtcag
     5041 ttcaagaaag ctttaggtgg tcttatgaag gctggtaaaa tcaagcagga ccagtttggg
     5101 acagagttga tttagggagg cttatgagaa aatcatttta cacttggctc atgaccgagc
     5161 gcaatcctaa aagtaacagt cccaaagcaa ttttggcaga cctcgctttt gaagaggcag
     5221 cttttccaaa acatacagat gattttgatg aggtcagtcg ctttttggag gagcatgcca
     5281 gtttctcttt taacctagga gattttgaca gcatttggca ggaatatcta gaacactagc
     5341 atttattcat tgggtttggg ctagtaattt ctccatccct ctgctataat aaaaagaaat
     5401 aaaaggatta gagaggttct ttatttgaag gaacattcaa tagacattca actgagtcat
     5461 ccagatgacc tgtttcatct ttttggttcc aatgaacgcc atcttcgttt gatggaagaa
     5521 gagcttgatg ttgtgattca tgctcgtacg gagattgtcc aggttttggg agaagagtct
     5581 gcctgtgagg aagcccgtca agttattcag gctttgatgg tcttggtaaa tcgtgggatg
     5641 accgttggta cgccagatgt agtcactgcg attagcatgg tcaaaaatga tgaaattgac
     5701 aagtttgtcg ccctttacga agaagaaatt atcaaagata atactgggaa acctatccgt
     5761 gtcaaaaccc tagggcaaaa gctttatgtg gacagtgtca aacagcatga tgtgaccttt
     5821 ggaattgggc cagcaggtac agggaagacc ttccttgcag tgaccttggc agtgactgcc
     5881 cttaaacgtg ggcaagtcaa gcgaattatc ctaactcgtc cagcggtgga agcgggagag
     5941 agtcttggat ttcttccggg tgatcttaag gagaaggtgg atccttacct tcgtcctgtt
     6001 tacgatgcct tgtatcagat tctggggaaa gaccaaacaa ctcgactcat ggagcgtgaa
     6061 attatcgaga ttgcgcccct tgcctatatg cgtggacgga ccttggatga tgcctttgtc
     6121 attctcgatg aggcgcaaaa tacgaccatc atgcagatga agatgttctt gactcgttta
     6181 ggttttcatt ctaagatgat tgtcaatgga gatattagtc agattgacct gccacgtaat
     6241 gtcaagtccg gtttgattga tgctcaagag aaactcaaga acatccatca gattgacttt
     6301 gttcattttt cagccaagga tgtggttcgc catccagttg tcgctcagat tatccgagcc
     6361 tatgaatatt ctactgaagt tgcacacgac tgattttgag gaagtttgcc tgcaaaagaa
     6421 tatacttttg agagaggaaa atccagttgt ataggctaaa ggttttatcc aaaggtttga
     6481 gacaacgatt aggcacgatg gaaagaactt ttatgtggct gatgacgatc agtgcatctt
     6541 cctgtgtcat aatcacaggg cacaagaaag taggaatttg aaaagatgat tgaccaacta
     6601 tctaagtatt acagttgtag gatactaact gaaaaggata ttccaagtat tttatcttta
     6661 tatgaaagta atcctctgta ttttcagcat tgtccaccag agccaaattt tgcaactgta
     6721 aaagaggaca tgctttgtct acctgaaggt aaagctaagg ctgataagtt ttttgttgga
     6781 ttttggaatg gatctgacct tgtggctgtt atagattttg tctatgcaca tcctgatgag
     6841 gagactgttt ttattggttt gtttatggtt gatcaagcct atcagaggaa agggattggt
     6901 agtcatattg tgacagaagc actagcttat tttgctaaga actttcgaaa ggcacgtttg
     6961 gcttatgtta agggaaatcc gcaatctcag catttttggg aaaagcaggg ctttaaatca
     7021 attggatgcg aggttaagca agaactctat acggttgtta tcgctgaaca gagcctagaa
     7081 gattagaaat ggcatcaagt aagaactatt tggaatttgt tttggaacaa ttatcaggat
     7141 tagatgatgt gacttaccgt tccatgatgg gggagtatat tctttacttc cgcggcaaga
     7201 ttattggcgg catttatgat gatcgctttt tagttaaacc cgtgcaagca gtcttagata
     7261 agattgacca atcttttttt gagtttccat acaaaggtgc caaagaaatg atttgagtgg
     7321 aagaacttga taataagatg tttctataag acctgatttt agctatgtat aaccaactgc
     7381 caacgcccaa acctaaaaag aaaaagcaag ggtgaacgaa gtaaaaaaga agtctgctaa
     7441 ggccctgtct ttgcacgggt aaaattttat atataaaaag aagctgggac taaagagctc
     7501 agcttccttt ggtttatata attgtcatta caagacgaag tggttgggcg aaactctgtt
     7561 gactttattc aatttagagt ttcttatgca caattgagtc tggaacgaaa gtctccagtt
     7621 gcaaagtata cagtacaata aaccaacgat gtaatagctg atgacacaaa gcacagtggg
     7681 taggacttgc gaagtcaccc ttttcttttc aaaatttata ctaaatcatt gatatcagtg
     7741 tagtcacgat taagtccttg agcaactggt aggctagtca agtaaccttg ataagtggtc
     7801 acaccttgac gcaagccttc atcttcagag attgcttgtg cgaatccttt gccagccaaa
     7861 gcttcgatat aaggaagagt gacattggtt agggcgatgg ttgaagtgcg agcaaccgca
     7921 ccagggatat tggcaacggc atagtggaga acaccgtgtt tttcatagac gggttcatcg
     7981 tgcgttgtca cacggtcagc tgtttcgata acgccacctt ggtcaacagc aacgtcaacg
     8041 atacagagcc tggacgcatt tgtttgacca tctcatctgt caccaattcc ggtgcttttg
     8101 caccagggat gagaatggct ccaatcacca catcagcatc tctcatactt gcttcaatgt
     8161 tgaatgaatt agatataaga gtttgaattt gacttccaaa gacttcttct agaactgaga
     8221 gacgcttggc actaatatct aaaatagtca cttgagcacc aagaccaagg gcgatgcggg
     8281 cagcatgtgt accgacgaca ccaccaccga tgatagttac ttttcctttt ggaacacctg
     8341 gtacaccacc aggtagaaca ccagagccac cagcttgctt agtaaggaag tgagctccga
     8401 tttaaacagc catacgacct gcaacctcac tcataggaac gaggagcggt agttgtcctt
     8461 gattgtcacg aacagtttca taggcaattc ctgttatttt tgctgctaac atagcatctg
     8521 ctaattctgg agcagcggcc atgtgcaagt aggtgaagag aagaagaacg tcgcgcaagt
     8581 aaccgtattc agaacttaaa ggttctttta ctttcacaac caactctgct gcccaagctt
     8641 caccagcagt agcgacaatc tcagctcctt gcttttgata gtcagcatca gtaaagccag
     8701 aaccgagacc agcatttgtt tcgataagga cacgatgacc acgactaact aagctatgaa
     8761 cacctgcagg tgtgagggcg acacggtttt cgttattttt aatttctttt gggattgaaa
     8821 gttctataat gaagttagcc accttacctt agtcaagaat tagatgtttc actatgtttg
     8881 agtaagttga tgatttcatt tgataacagg tttgaaactg ttaggctagg tggccaaggc
     8941 taatcatagc cttggtttag ctgaaaaaac aggttcaagg gttcctgttg tcaaatgaaa
     9001 tgtgatttaa ggtataagaa aacacctctg tgctatactt gttgttcacc acaaacacaa
     9061 ggaaaggcac agagatgcaa gaacattata ccccaaaagg gaaacatttg acaatagata
     9121 accgtcgctt gattgagcgg tggaagaatg aaaataagtc caatcgtgaa attgcaggct
     9181 tgttaggaaa ggcgcctcaa acgattcata atgaagtcaa aagaggtaca actttacaac
     9241 aagtgagaaa agggctatac aaaaaggtct attctgccga ttacgcacaa actgtttacc
     9301 aattcaatcg aaaacggtcg gtgaaaaagt taattttaac aaaggaaatc agagagaaga
     9361 tcttacacta tcataagcaa aaattttcgc ctgaaatgat ggttaacaag aagcaagtga
     9421 aagttggtat ttcaaccatc tactactggt ttcataatgg tcatttagga ttgacgaagg
     9481 ccgacatgct ttatcccaga aaaaggaaag gtgtcaagaa gcaagctagt ccgaacttta
     9541 agccggcagg taaatctatc gaagaaggtc ctgacgttat taatcttcgc ttggaaaatg
     9601 gtcattatga aattgatacc gtcctactga ctaagataaa aaattattgc ctgttagtct
     9661 taaccgaccg gcggagcaga caccaaatta taaggttaat tccaaataaa actgctgaat
     9721 ctgtcaatca ggcgcttacg ttactattag gggagcatcg tattctgtcc attactgcag
     9781 ataatggttc ggagttcaaa cgattgtctg aggtatttcc tgaggaacat atctactacg
     9841 cacatgctta ctcttcatgg gagagaggtt caaatgaaaa tcataatcga ttaattcgga
     9901 gatggttacc taaaggaacc aagaaaacga ctccgaaaga agtagctttt atcgaaaatt
     9961 ggattaacaa ctaccctaaa aaatgcttgg actacaagtc gccaagtgaa tttcttttgg
    10021 gtggctaact tcaacttgaa atttgggatt tcttttggga ttccgattaa cattgagata
    10081 acctaccttt caattgacgg tcttgttttg gttgtcacat tccagttcat aaatcaaaaa
    10141 tgtgacggtt tcattgtata tgaaaccgct tcaaaaatca agaaaaactt gtcatccaaa
    10201 tttttttatg ctagactagt gaaaatcaag ctctaatgga gggaaaagta tggaatcaat
    10261 atttgtgaaa tttgcccagt atccgtctat agaaacggag cgtttattgc ttagacctgt
    10321 aactttggat gatgcggaag caatgtttga ctatgcctcg gacaagggta atacacgtta
    10381 cacttttcca accaatcaaa gcttggaaga aaccaagaat aacattgctc agttctactt
    10441 ggctaatccc ttgggacgtt gggggattga attaaaagat aacggcaagt ttatcggaac
    10501 tattgactta cacaagattg atcctgttct taagaaggca gctattggct acattatcaa
    10561 taaaaagtat tggaatcaag gattaacgac agaagccaat cgtgctgtga ttgagctagc
    10621 ttttgagaag atagggatga ataagttgac tgcccttcac gataaggcta atcccgcgtc
    10681 aggaaaggtc atggagaaat caggcatgcg tttttcccat gcagaaccat atgcttgtat
    10741 ggaccagcat gaaaaaggcc gaatcgtgac aagagttcat tatgtcttga ccaaggaaga
    10801 ctattttgca aataaataag cagttgaaaa gaaatttttc gactgttttt tcttcctctt
    10861 acgaataatc taagagagga gaaaatatgg aagcaattat cgagaaaatc aaagagtata
    10921 aaatcatcgt catctgtact ggtctgggct tgcttgtagg aggatttttc ctgctaaagc
    10981 cagctccaca aacatctgtc aaagagacga atttgcaggc tgaagttgca gctgtttcca
    11041 aggactcatc gaccggaaag gaagtgaaca aggaagagaa ggaagaacca gttgaacaag
    11101 atctaatcac agtagatgtc aaaggtgctg tcaaatcgcc agggatttat gacttgcctg
    11161 taggtagtcg agtcaatgat gctgttcaga aggctggtgg cttgacagag caagcagaca
    11221 gcaagtcgct caatctagct cagaaagtta gtgatgaggc tctggtttac gttcctacta
    11281 agggagaaga agcagttagt caacagactg gttcggggac agcttcttca acaagcaagg
    11341 aaaagaaggt caatctcaac aaggccagtc tggaagaact caagcaggtc aagggactgg
    11401 gaggaaaacg agctcaggac attattgacc atcgtgaggc aaatggcaag ttcaagtcag
    11461 tagacgagct caagaaggtc tctggcattg gtgccaagac catagaaaaa cttaaagact
    11521 atgttacagt ggattaagaa tttctctatt cccctaattt acctgagttt tctgttacta
    11581 tggctttact acgccatttt ctcagtatcc tattttgctt tgttgggttt tgtttttctg
    11641 ctagtctgcc tctttatcca atttccttgg aaatcagcag gtaaagttct agtgatttgt
    11701 ggagtctttg gcttctggtt tctgtttcaa acttggcaac agagtcaagt gagtcaaaat
    11761 ttggtggatt ctgttgaaag ggtacggatt ttaccagaca ctattaaggt taacggtgac
    11821 agtctgtcct ttcgtggtaa gtctgatggt cgtgctttcc aagtctatta taaactccag
    11881 tccgaggagg agaaagaagc ctttcaagct ttaactgacc ttcatgagat aggactagaa
    11941 gggaagcttt cggagccaga agggcagaga aattttggtg gctttgacta ccaaggctat
    12001 ctgaagactc agggaattta ccagacactc aatattaaaa gaatccagtc actccaaaag
    12061 gttggcagtt gggatatagg tgaaaacctg tccagtttac gtcgaaaggc tgtggtttgg
    12121 attaagatgc acttcccaga ccctatgcgc aattacatga caggactctt gctaggacat
    12181 ctggacaccg attttgagga gatgaatgag ctttattcca gtttaggaat tattcacctt
    12241 tttgccttgt caggtatgca ggtagggttt ttcatggacg gatttaagaa actactttta
    12301 cgattgggct tgacacaaga aaagttgaag tggctgactt atcccttttc ccttatctat
    12361 gcgggactaa ctggattttc agcatcggtt attcgcagtc tcttgcaaaa gctactggct
    12421 caacatgggg ttaagggctt ggataatttt gccttgacgg tgcttgtcct ctttattgtc
    12481 atgccaaact ttttcttgac agcaggagga gtcttgtcct gtgcttatgc ttttatcctg
    12541 accatgacca gcaaagaagg ggaggggctc aaggctgtta ctagtgaaag tctagtcatc
    12601 tccttgggca tattgcccat tctatccttc tattttgcgg aatttcaacc ttggtctatc
    12661 cttttgacct ttgtcttttc ctttcttttt gacttggtct tcttaccgct cttgtctatc
    12721 ttatttgtcc tttcctttct ctatccagtc attcagctga actttatctt tgaatggtta
    12781 gagggcatta ttcgcttggt ctcgcaggtg gcaagtagac cgcttgtctt tggacaaccc
    12841 aatgaatggc ttttaatcct attgttaatt tctttggctt tggtctatga tttgaggaaa
    12901 aacattaaaa agctaacggt attgtgctta ttgattacag ggctctttct cctgaccaag
    12961 catccactgg aaaatgaaat caccatgctg gatgtggggc aaggagaaag tattttccta
    13021 cgggatgtaa ctgggaaaac cattctcata gatgtaggtg gtaaggcaga atcttataag
    13081 aaaatcaaaa aatggcaaga aaagatgacg accagcaatg cccagcgaag cttgataccc
    13141 tatctcaaaa gtcgaggagt agctaagatt gaccagctaa ttttgactaa cacggacaag
    13201 gagcatgttg gagatttgtc agagatgacc aaggctttcc atgtagggga gattctagta
    13261 tcaaaagaca gtctgaaaca gaaggaattt gtggcagaac tacaggcgac tcaaacaaag
    13321 gtgcgtagta tgatagtagg ggagaacttg cccatttttg gaagtcagtt agaagttcta
    13381 tctccaagga aaatgggaga tggaggacac gatgataccc tagttctgta tgggaaattc
    13441 ttggataagc aatttctctt cacgggaaat ttggaggaga aaggagagaa ggacttgctg
    13501 aagcactatc cagacttgaa agtaaatgtt ttgaaagcta gccaacatgg caataaaaaa
    13561 tcatcaagtc cagcctttct agaaaaactc aaaccagagc ttactcttat ctcagttgga
    13621 aagagcaatc gaatgaaact cccccatcag gaaacattga cacgactgga aggtatcaat
    13681 agcaaagttt atcgaactga ccagcaagga gctatacgtt ttaaggggtt ggatagttgg
    13741 aaaatcgaaa gtgttcgata ggaaggataa atgttgtaga ttagtgaaat aaactaaaaa
    13801 tttgttgcat aataatgata aaaacggcat aatgaaaacg tattcaatat tgaggatata
    13861 aaatcattaa aaatcagcaa aagttgtttt agttagttag tttataatct attggtcttc
    13921 ttcagtccag tgtatctgct gtgacagtca ctaaaagtta caagtatgat tggaatacgg
    13981 ttttggaata tagtaccaac tatcacgacc atcagtatgc ttggattccg gaatggtctc
    14041 gttattacag ctattctgag tataaagttg gcggaggctg gaactacgct cgttatgagg
    14101 tcataaacta ctatactgga agttattaat ccttaaagag tgagaaaagg agggctggat
    14161 atgttaaatc ttactcatgt taccttaaaa acgcgacaag tcatcttgca agatgcggat
    14221 tttaccttta aaaagggtag gatttatggc cttcttgcta tcaatggctc gggaaagacg
    14281 acactattcc gagctatgag caagttgctt ccccttagta gtggacacat cgcagttcct
    14341 ccttctttgt tttattatga gagcgttgaa tggctggatg gaaacttaag tgggatggac
    14401 taccttcgtc tcataaaaaa catctggaag tcagacctaa acttgagaga tgaaatcgcc
    14461 tactgggaaa tgactgacta tatcagtctt cccatccgca agtattcctt agggatgaag
    14521 caacgcttgg tgattgctat gtattttctc agtcaggcca aatgctggct catggatgag
    14581 attacaaatg gcttagacga gtattatcga cagaagtttt ttgataggct agcacaaatc
    14641 gatagacaag aacagctggt tcttttaagt tcccactata aggaagagtt ggttgatatc
    14701 tgcgatagag tagtaaccat tcatcagggg cagatagaag aggtttagtt tatgaaagat
    14761 gttagtctat ttttattgaa aaaagttttc aaaagtcgtt taaactggat tatcttagct
    14821 ttatttgtat ctgtactcgg tgttaccttt tatttaaata gtcagactgc aaactcacac
    14881 agcttggaga gcaggttgga aagtcgcatt gcagccaacg agagggctat caatgaaaat
    14941 gaagagaaac tctcccaaat gtctgatacc agctcggagg aataccagtt tgctaaaaat
    15001 aatttagacg tgcaaaaaaa tcttttgacg cgaaagacag aaattctaac tttactaaaa
    15061 gaagggcgct ggaaagaagc ctactatctg cagtggaaag atgaagagaa gaattatgaa
    15121 tttgtatcaa atgacccgac tgctagctct ggcttaaaaa tgggggttga ccgcgaacgg
    15181 aagatttacc aagccctgta tcccttgaac ataaaagcac atactttgga gtttccgacc
    15241 cacgggattg atcagattgt ctggatttta gaggttatca tcccaagctt gtttgtggtt
    15301 gcgattattt ttatgctaac acaactattt gcagaaagat atcaaaatca tctggacaca
    15361 gctcacttat atcctgtttc aaaagtgaca tttgcaatat cctctcttgg agttggagtg
    15421 ggatatgtaa ctgtgctgtt tatcggaatc tgtggctttt cttttctagt gggaagtctg
    15481 ataagtggtt ttggacagtt agattatccc tacccaattt atagcttagt gaatcaagaa
    15541 gtaactattg ggaaaataca agatgtatta tttcctggct tgctcttagc tttcttagcc
    15601 tttatcgtca ttgtggaagt tgtgtacttg attgcttact ttttcaagca aaaaatgcct
    15661 gtcctctttc tttcactcat tgggattgtt ggcttattgt ttggcatcca aacaattcag
    15721 cctcttcaaa ggattgcaca tctgattccc tttacttact tgcgttcagt ggagatttta
    15781 tctggaagat tacctaagca gattgataat gtcgatctaa attggagcat gggaatggtc
    15841 ttacttcctt gcctgattat ctttttgcta ttgggaattc tatttattga aagatgggga
    15901 agttcacaga aaaaaagaat tttttaatag attctagctt tcctataggt agggaaaata
    15961 agtaaaaact aacatagaga gggaatcaac ttgattctct ctttttgatt cgaaaaccaa
    16021 accaaaatac aaacacaaac ttttcaaaaa ataacttttt atcttgacaa gagctagaaa
    16081 acttggtatc atataaaagt tgagaaaagc agaagtgaga gcttctcgcc ttgtgacatt
    16141 aagttgcctg gccctacgga tgaaaagttt cgaagaaacg ctatcataac gtgcgggctt
    16201 gtatatttac gagtccgcta ttgtttttct ctaataaaac aaaagaggtg aaaaccatag
    16261 caaagcaaga cttattcatc aatgatgaga ttcgtgtacg tgaagttcgc ttgattggtc
    16321 ttgaaggaga acagctaggc atcaagccac tcagtgaagc gcaagctttg gctgataacg
    16381 ctaatgttga cctagtattg attcaacccc aagccaaacc gcctgttgca aaaattatgg
    16441 actacggtaa gttcaaattt gagtaccaga agaagcaaaa agaacaacgt aaaaaacaaa
    16501 gcgttgttac tgtgaaagaa gttcgtctaa gtccaactat tgacaagggt gactttgata
    16561 caaaacttcg taatgcacgt aagttccttg aaaaaggaaa taaagttaag gtatctattc
    16621 gctttaaggg tcgtatgatt acccataaag agattggtgc aaaagtttta gccgagtttg
    16681 ctgaagcaac tcaagatatt gccatcatcg aacaacgtgc taaaatggat ggacgtcaaa
    16741 tgttcatgca attggcgcca gcgactgaca aaaaataatt gtcagaaagt taaataaagg
    16801 agaaaaaatt atgccaaaac aaaaaacaca ccgcgcatca gctaaacgtt tcaaacgtac
    16861 aggttctggt ggacttaaac gtttccgtgc ttacacttct caccgtttcc acggaaaaac
    16921 taagaaacaa cgtcgtcatc ttcgtaaagc atctatggtg cattcaggag attacaaacg
    16981 tatcaaagca atgcttactc gcttgaaata atagcttgac tgtaagtaaa caattctagg
    17041 aaatatttgg aggaaataaa acatggcacg tgttaaaggt ggcgttgtat cacgcaaacg
    17101 tcgtaaacgt attcttaaat tagcaaaagg ttactatgga gctaaacaca tcttgttccg
    17161 tactgcaaaa gaacaagtaa tgaactctta ctactatgca taccgtgacc gtcgtcagaa
    17221 aaaacgtgac ttccgcaaat tgtggatcac tcgtatcaat gcggcagctc gtatgaacgg
    17281 actttcatac tcacaattga tgcatggttt gaaattggct gagatcgaag ttaaccgtaa
    17341 aatgcttgct gacttggctg ttaacgatgc agtagctttc acagctcttg cagatgcagc
    17401 taaagcaaaa cttggtaaat aacaactgat gagactggaa caggattgtc cagtcttttt
    17461 aaaaataaag gagaaaaata tggcttcaaa aatgctacac acttgcttac gagtagaaaa
    17521 tcttgaaaaa tcaatcgcat tttatcaaga tgcttttggt tttaaagaat tgcgtcgcag
    17581 agattttcca gaccatgcct tcacgattgt ctatctaggt cttgagggtg atgactatga
    17641 gttggagttg acttataact acgatcacgg tccgtatgtg gttggtgatg ggtttgccca
    17701 tatcgccctc agtacacctg atcttgaggc acttcatcaa gaacacagtg caaaaggcta
    17761 tgaagtgact gagccaaatg gtctaccagg aactacacca aactattact ttgtcaaaga
    17821 ccctgatgga tataaggtcg aagtcattcg tgaaaaataa tttcgtgcaa gttaagtaga
    17881 atattcgttt tttacttgac tttttttagc tgaaagagta tactaataaa aattcaacct
    17941 ttaaggggag tccagagaga ctcacaaggt gtcagataaa agaatagtgc aattttctag
    18001 aggagacttt ttgagtgtgc tctcttgtgt tgtacgattt taactgaggc cttgcactag
    18061 caaggtcttt tctttatctg gtccccttaa aatttaagga ggaaaagtta tgaatcccac
    18121 atgtaagaag cgtttgggtg tcattcggtt ggaaaccatg aaggtggttg cacaagagga
    18181 aatcgcgcca gcaatctttg aattagtcct agaaggagaa atggttgaag ccatgcgagc
    18241 aggccaattt cttcatctgc gtgtaccaga cgatgcccat cttttacgtc gtcctatttc
    18301 aatttcgtct attgacaagg caaacaagca gtgtcacctc atttatcgga ttgagggagc
    18361 tgggactgca attttttcaa ccttaagtca gggagacact cttgatgtga tggggcctca
    18421 gggaaatggt tttgacttgt ctgaccttga tgagcagaat caggttctcc ttgttggtgg
    18481 tgggattggt gttccaccct tgcttgaggt ggccaaggaa ttgcatgaac gtggagtgaa
    18541 agtagtgaca gtcctcggtt ttgctaataa ggatgctgtt attttgaaaa cggaattggc
    18601 tcagtatggt caggtctttg taacgacaga tgatggttct tatggcatca agggaaatgt
    18661 ttccgttgtt atcaatgatt tagatagtca gtttgatgct gtttactcgt gtggggctcc
    18721 aggaatgatg aagtatatca atcaaacctt tgatgatcac ccaagagcct atttatctct
    18781 ggaatctcgt atggcttgtg ggatgggagc ttgctatgcc tgtgttctaa aagtaccaga
    18841 aagcgagacg gtcaaccaac gcgtctgtga agatggtcct gttttccgca caggaacagt
    18901 tgtattataa ggagaaaatt atgactacaa atcgattaca agtgtctcta cctggtttgg
    18961 atttgaaaaa tccgattatt ccagcatcag gttgttttgg ctttggacaa gagtatgcca
    19021 agtactatga tttaaacctt ttaggttcta ttatgatcaa ggcgacaacc cttgaaccac
    19081 gttttgggaa cccaactcca agagtagcag agacgcctgc tggtatgctc aatgcaattg
    19141 gcttgcaaaa tcctggttta gaggttgttt tggctgaaaa gctaccttgg ctggaaagag
    19201 aatatccaaa tcttcctatt attgccaatg tagctggttt ttcaaaacaa gagtatgcag
    19261 ctgtttctca tgggatttcc aaggcaacta atgtaaaagc tatcgagctc aatatttctt
    19321 gtcccaatgt tgaccactgt aatcatggac ttttgattgg tcaagatcca gatttggctt
    19381 atgatgtggt gaaagcagct gtggaagcct cagaagtgcc agtttatgtc aaattaaccc
    19441 cgagtgtgac cgatatcgtt actgtcgcaa aagctgcaga agatgcggga gcaagtggct
    19501 tgaccatgat caatactctg gttggaatgc gctttgacct caaaaccaga aaaccaatct
    19561 tggccaatgg aacaggtgga atgtcaggtc cagcagtctt tccagtagcc ctcaaactca
    19621 tccgccaagt agcccaaaca acagacctgc ctatcattgg aatgggggga gtggattcgg
    19681 ctgaagctgc cctagaaatg tatctggctg gggcatctgc tatcggagtt ggaacagcta
    19741 actttaccaa tccttatgcc tgccctgaca tcatcgaaaa tttaccaaaa gtcatggata
    19801 aatacggtat tagcagtctg gaagaactcc gtcaggaagt aaaagagtct ctgaggtaaa
    19861 ctgcaatcaa tctgttcttg attttttatt agtttgtaat atgaatttag gagaattttg
    19921 gtacaataaa ataaataaga acagaggaag aaggttaatg aagaaagtaa gatttatttt
    19981 tttagctctg ctatttttct tagctagtcc agagggtgca atggctagtg atggtacttg
    20041 gcaaggaaaa cagtatctga aagaagatgg cagtcaagca gcgaatgagt gggtttttga
    20101 tactcattat caatcttggt tctatataaa agcagatgct aactatgctg aaaatgaatg
    20161 gctaaagcaa ggtgacgact atttttacct caaatctggt ggctatatgg ccaaatcaga
    20221 atgggtagaa gacaagggag ccttttatta tcttgaccaa gatggaaaga tgaaaagaaa
    20281 tgcttgggta ggaacttcct atgttggtgc aacaggtgcc aaagtaatag aagactgggt
    20341 ctatgattct caatacgatg cttggtttta tatcaaagca gatggacagc acgcagagaa
    20401 agaatggctc caaattaaag ggaaggacta ttatttcaaa tccggtggtt atctactaac
    20461 aagtcagtgg attaatcaag cttatgtgaa tgctagtggt gccaaagtac agcaaggttg
    20521 gctttttgac aaacaatacc aatcttggtt ttacataaaa gaaaatggaa actatgctga
    20581 taaagaatgg attttcgaga atggtcacta ttattatcta aaatctggtg gctacatggc
    20641 agccaatgaa tggatttggg ataaggaatc ttggttttat ctcaaatttg atgggaaaat
    20701 agctgaaaaa gaatgggtct acgattctca tagtcaagcc tggtactact tcaaatccgg
    20761 tggttacatg acagccaatg aatggatttg ggataaggaa tcttggtttt acctcaagtc
    20821 tgatggcaaa atggctgaaa aagaatgggt ctacgattct catagtcaag cctggtacta
    20881 cttcaaatct ggtggttaca tggcgaaaaa tgagacagta gatggttatc agcttggaag
    20941 cgatggtaaa tggcttggag gaaaagctac aaatgaaaat gctgcttact atcaagtagt
    21001 gcctgttaca gccaatgttt atgattcaga tggtgaaaag ctttcctata tatcgcaagg
    21061 tagtgtcgta tggctagata aggatagaaa aagtgatgac aagcgcttgg ctattactat
    21121 ttctggtttg tcaggctata tgaaaacaga agatttacaa gcgctagatg ctagtaagga
    21181 ctttatccct tattatgaga gtgatggcca ccgtttttat cactatgtgg ctcagaatgc
    21241 tagtatccca gtagcttctc atctttctga tatggaagta ggcaagaaat attattcggc
    21301 agatggcctg cattttgatg gttttaagct tgagaatccc ttccttttca aagatttaac
    21361 agaggctaca aactacagtg ctgaagaatt ggataaggta tttagtttgc taaacattaa
    21421 caatagcctt ttggagaaca agggcgctac ttttaaggaa gccgaagaac attaccatat
    21481 caatgctctt tatctccttg cccatagtgc cctagaaagt aactggggaa gaagtaaaat
    21541 tgccaaagat aagaataatt tctttggcat tacagcctat gatacgaccc cttacctttc
    21601 tgctaagaca tttgatgatg tggataaggg aattttaggt gcaaccaagt ggattaagga
    21661 aaattatatc gataggggaa gaactttcct tggaaacaag gcttctggta tgaatgtgga
    21721 atatgcttca gacccttatt ggggcgaaaa aattgctagt gtgatgatga aaatcaatga
    21781 gaagctaggt ggcaaagatt agtactataa gtgaatatga tttgagtgaa tagtaagtta
    21841 aaaatcctga tttcaagtaa aatcaggatt ttttcatgga tgcaattttt ttggagtctg
    21901 gtgtgacgcg gagggtcttt tgtcctgtgt aagtgacaaa gccgggtttt ccaccagttg
    21961 gtttattgag ttttttgact tcaatcatat ctacctgcac cagattcgac aggcgccctt
    22021 gagagaagta ggcagctaac tctgctgcgt ctgtcttgac tgcatcagat gggtcaagat
    22081 ttcctgagat gacaacatgg cttccaggaa tgtccttagc atggaaccaa agttcctcct
    22141 tgcgggccat tttaaaggtc aattcctcat tttgaagatt gtttcgtccg acatagatga
    22201 tggttttgcc atcgcttgct agatattgtt ctagtttttt gcgtttctgg attttctccc
    22261 gttgtcttct gcggataaaa cctgtttgaa tcaattcttc acggatttca gcgatttctt
    22321 ccagtccagc ttggttgagg acggtttcta cactttccag atagagaata gtggctttgg
    22381 tttcttcaat caaatcagtc aagtatttga cagcttcttt gagtttctga taccgtttaa
    22441 aatagcgttg ggcattctgg ttgggagtca gagccttatc aagcgcaatc atgataggtt
    22501 ggttggtata gtagttgtct aggataacct ggtcttggtc gttaggcact tggtggagga
    22561 aggttgtcag caattctcct ttttgacgaa attcttcagc gttgtctgtc gccagtaact
    22621 ctttttcctg ttttttgagt ttgtgtcggt ttttctgaag ttcattttca acacgacgaa
    22681 tcagttcact ggcctgctgt ttgacgcggt cgcgctcagc cttatcctta tagtaggtgt
    22741 ccaacaaatc agaaagattt gcaagaggct ctcccacctg atttgcaaaa ggaactggac
    22801 tgaaggaagt ctcagtcaag catggcttgg tttcttgatt gaaaaaattt cggaaagcgg
    22861 aaagtttttc actaaccagt atcctttcca attcatttgc cgtatcgcgt cccagacctt
    22921 gaaagaggct ttgaagattt tttgctgtta gttcttgggt ttgcaggatt tcaaagagct
    22981 tttcatcctt gatagtaaaa ggattgagag attttgtact tggcggagcg atataggtcg
    23041 atcctggaag taaggtgcgg tagctatttt gtgaaaagcc gacgtgtttg ataacttcga
    23101 ggattttatg actgctttta tcgaccagta gaatattact gtgtttcccc ataatttcga
    23161 taatcaaggt agcctggata tggtctccaa tctcgttttt attggaaact gtaatttcca
    23221 caatacggtc attttccact tgctcaatcg actcaatcag ggccccctgc aaatactttc
    23281 tcaaaaccat gataaaggta gaaggttggg ctggattttc aaaagtcgtt tgggtcagct
    23341 gaatgcgtcc aaaaactgga tgggcagaaa ggagcaggcg atggctttgg cgattgctgc
    23401 ggatttgcaa gaccaactct tgttcaaaag gctgattgat tttctggatg cgaccattca
    23461 ctaattcgct tcgcaattcc tcaactatgt ggtgtaaaaa aaatccgtca aatgacatcg
    23521 ttctctcctt gtgattgtat tccatagtat tatatcaaaa aggtagaata aaatcatgga
    23581 aatgtggtat aataaagcca agtaaagaga aacgagaagc acatgtatat tgaaatggta
    23641 gatgaaactg gtcaagtttc aaaagaaatg ttgcaacaaa cccaagaaat tttggaattt
    23701 gcagcccaaa aattaggaaa agaagacaag gagatggcag tcacttttgt gaccaatgag
    23761 cgtagtcatg aacttaatct ggggtaccgt gacaccgacc gtccgacaga tgtcatcagc
    23821 cttgagtata aaccagaatt ggaaattgcc tttgacgaag aggatttgct tgaaaatcca
    23881 gaattggcag agatgatgtc tgagtttgat gcctatattg gggaattgtt catctctatc
    23941 gataaggctc atgagcaggc cgaagaatat ggtcacagct ttgagcgtga gatgggcttc
    24001 ttggcagtac acggcttttt acatattaac ggctatgatc actatactcc ggaagaagaa
    24061 gcggagatgt tcggtttaca agaagaaatt ttgacagcct atggactcac aagacaataa
    24121 acgaaaatgg aaaaatcgtg acctgatatc cagtttagaa tttgccatca cagggatttt
    24181 tactgctatc aaggaagaac gcaatatgcg aaaacatgca gtgacggctc tagtggtcat
    24241 ccttgcaggt tttggttttc aggtgtcacg aatcgaatgg ctctttctcc tattgagcat
    24301 tttcttggtg gtagcctttg aaattatcaa ttctgctatc gaaaatgtgg tggatttggc
    24361 cagtcactat cacttttcca tgctggctaa aaatgccaag gatatggcgg ctggcgcggt
    24421 attagtggtt tctctttttg cagccttaac aggcgcattg atttttctcc cacgaatctg
    24481 ggatttatta ttttaaacag taagaggaaa ttatgacttt taaatcaggc tttgtagcca
    24541 ttttaggacg tcccaatgtt gggaagtcaa cctttttaaa tcacgttatg gggcaaaaga
    24601 ttgccatcat gagtgacaag gcgcagacaa cgcgcaataa aatcatggga atttacacga
    24661 ctgataagga gcaaattgtc tttatcgaca caccagggat tcacaaacct aaaacagctc
    24721 tcggagattt catggttgag tctgcctaca gtacccttcg cgaagtggac actgttcttt
    24781 tcatggtgcc tgctgatgaa gcgcgtggta agggggacga tatgattatc gagcgtctca
    24841 aggctgccaa ggttcctgtg attttggtgg tgaataaaat cgataaggtc catccagacc
    24901 agctcttgtc tcagattgat gacttccgta atcaaatgga ctttaaggaa attgttccaa
    24961 tctcagccct tcagggaaat aacgtgtctc gtctagtgga tattttgagt gaaaatctgg
    25021 atgaaggttt tcaatatttc ccgtctgatc aaatcacaga ccatccagaa cgtttcttag
    25081 tttcagaaat ggttcgcgag aaagtcttgc acctaactcg tgaagagatt ccgcattctg
    25141 tagcagtagt tgttgactct atgaaacggg acgaagagac agacaaggtt cacatccgtg
    25201 caaccatcat ggtcgagcgc gatagccaaa aagggattat catcggtaaa ggtggcgcta
    25261 tgcttaagaa aatcggtagc atggcccgtc gtgatatcga actcatgcta ggagacaagg
    25321 tcttcctaga aacctgggtc aaggtcaaga aaaactggcg cgataaaaag ctagatttgg
    25381 ctgactttgg ctataatgaa agagaatact aagtagaggt gggctcatgc ctgcttcttg
    25441 tttttacaga aggaggactt atgcctgaat tacctgaggt tgaaaccgtt tgtcgttgct
    25501 tagaaaaatt gattatagga aagaagattt cgagtataga aattcgctac cccaagatga
    25561 ttaagacgga tttggaagag tttcaaaggg aattgcctag tcagattatc gagtcaatgg
    25621 gacgtcgtgg aaaatatttg cttttttatc tgacagacaa ggttttgatt tcccatttgc
    25681 ggatggaggg caagtatttt tactatccag accaaggacc tgaacgcaag catgcccatg
    25741 ttttctttca ttttgaagat ggtggcacgc ttgtttatga ggatgttcgc aagtttggaa
    25801 ccatggaact cttggtgcct gaccttttag acgcctactt tatttctaaa aaattaggtc
    25861 ctgaaccaag cgaacaagac tttgatttac aggtctttca atctgccctt gccaagtcca
    25921 aaaagcctat caaatcccat ctcctagacc agaccttggt agctggactt ggcaatatct
    25981 atgtggatga ggttctctgg cgagctcagg ttcatccagc tagatcttcc cagactttga
    26041 cagcagaaga agcgacagct attcatgacc aaaccattgc tgttttgagc caggctgttg
    26101 aaaaaggcgg ttccaccatt cggacctata ccaatgcctt tggggaagat ggaagcatgc
    26161 aggattttca tcaggtctat gataagactg gtcaaggatg tgtacgctgt ggtaccatca
    26221 ttgagaaaat tcaactaggt ggacgtggaa cccacttttg tccaaactgt caaaggaggg
    26281 actgatggga aaaatcatcg gaatcactgg gggaattgcc tctggtaagt caactgtgac
    26341 aaattttcta agacagcaag gctttcaagt agtggatgcc gacgcagtcg tccaccaact
    26401 acagaaacct ggtggtcgtc tgtttgaggc tctagtacag cacttttggc aagaaatcat
    26461 tcttgaaaac ggagaactca atcgccctct cctagctagt ctcatctttt caaatcctga
    26521 tgaacgagaa tggtctaagc aaattcaagg ggagattatc cgtgaggaac tggctacttt
    26581 gagagaacag ttggctcaga cagaagagat tttcttcatg gatattcccc tactttttga
    26641 gcaggactac agcgattggt ttgctgagac ttggttggtc tatgtggacc gagatgccca
    26701 agtagaacgc ttaatgaaaa gggaccagtt gtccaaagat gaagctgagt ctcgtctggc
    26761 agcccagtgg cctttagaaa aaaagaaaga tttggccagc caggttcttg ataataatgg
    26821 caatcagaac cagcttctta atcaagtgca tatccttctt gagggaggta ggcaagatga
    26881 cagagattaa ctggaaggat aatctgcgca ttgcctggtt tggtaatttt ctgacaggag
    26941 ccagtatttc tttggttgta ccttttatgc ccatcttcgt ggaaaatcta ggtgtaggga
    27001 gtgagcaagt cgctttttat gcaggcttag caatttctgt ctctgctatt tccgcggcgc
    27061 tcttttctcc tatttggggt attcttgctg acaaatacgg ccgaaaaccc atgatgattc
    27121 gggcaggtct tgctatgact atcactatgg gaggcttggc ctttgtccca aatatctatt
    27181 ggttaatctt tcttcgttta ctaaacggtg tatttgcagg ttttgttcct aatgcaacgg
    27241 cactgatagc cagtcaggtt ccaaaggaga aatcaggctc tgccttaggt actttgtcta
    27301 caggcgtagt tgcaggtact ctaactggtc cctttattgg tggctttatc gcagaattat
    27361 ttggcattcg tacagttttc ttactggttg gtagttttct atttttagct gctattttga
    27421 ctatttgctt tatcaaggaa gattttcaac cagtagccaa ggaaaaggct attccaacaa
    27481 aggaattatt tacctcggtt aaatatccct atcttttgct caatctcttt ttaaccagtt
    27541 ttgtcatcca attttcagct caatcgattg gccctatttt ggctctttat gtacgcgatt
    27601 tagggcagac agagaatctt ctttttgtct ctggtttgat tgtgtccagt atgggctttt
    27661 ccagcatgat gagtgcagga gtcatgggca agctaggtga caaggtgggc aatcatcgtc
    27721 tcttggttgt cgcccagttt tattcagtca tcatctatct cctctgtgcc aatgcctcta
    27781 gcccccttca actaggactc tatcgtttcc tctttggatt gggaaccggt gccttgattc
    27841 ccggggttaa tgccctactc agcaaaatga ctcccaaagc cggcatttcg agggtctttg
    27901 ccttcaatca ggtattcttt tatctgggag gtgttgttgg tcccatggca ggttctgcag
    27961 tagcaggtca atttggctac catgctgtct tttatgcgac gagcctttgt gttgccttta
    28021 gttgtctctt taacctgatt caatttcgaa cattattaaa agtaaaggaa atctagtgcg
    28081 agtaaaaatt aatctcaaat gctcctcttg tggcagtatc aattacctaa ccagtaaaaa
    28141 ttcaaaaacc catccagaca agattgaggt tttaaagtat tgtcccaagg aaagaaaagt
    28201 aaccctacat cttgaatcta agtagatttt atggtaaaat aatagggatt ttaaggagtt
    28261 tgatatgtat aacctattat taaccatttt attagtatta tctgttgtga ttgtgattgc
    28321 aattttcatg caaccaacca aaaaccaatc cagcaatgta tttgatgcca gttcaggtga
    28381 tttgtttgaa cgtagtaaag ttcgcggttt tgaagctgta atgcagcgtt tgacagggat
    28441 tttagtcttt ttctggctag ccattgcctt agcattgacg gtattatcaa gtagataaga
    28501 aaataatggg caggactagg tctttgcctc tttttatttt taaaggatgt ttgagaaggt
    28561 tttacagtaa aagaaaatta aaaaatctag aaagaaaata tgaaagatag aataaaagaa
    28621 tatttacaag acaagggaaa ggtgactgtt aatgatttgg ctcaggcttt gggaaaagac
    28681 agttccaagg attttcgtga gttgattaaa accttgtcct taatggaaag aaagcaccaa
    28741 attcgttttg aagaagatgg tagtctgaca ttagaaatta agaaaaaaca tgagattacc
    28801 ctcaagggga tttttcatgc ccataaaaat ggctttggct ttgttagtct ggaaggcgag
    28861 gaggacgacc tttttgtagg gaaaaatgat gtcaactatg ctattgatgg tgataccgtc
    28921 gaggtagtga ttaagaaagt cgctgaccgc aataagggaa cagcagcaga agccaaaatt
    28981 attgatatcc tagaacacag tttgacaaca gttgtcgggc aaatcgttct ggatcaggaa
    29041 aaacctaagt atgctggcta tattcgttca aaaaatcaga aaatcagtca accgatttat
    29101 gttaagaaac cagccctaaa attagaagga acagaagttc tcaaagtctt tatcgataaa
    29161 tacccaagca agaaacatga tttctttgtc gcgagtgttc tcgatgtagt gggacactca
    29221 acggatgtcg gaattgatgt tcttgaggtc ttggaatcaa tggacattgt atccgagttt
    29281 ccagaagctg ttgttaagga agcagaaagt gtgcctgatg ctccgtctca aaaggatatg
    29341 gaaggtcgtc tggatctaag agatgaaatt acctttacca ttgacggtgc ggatgccaag
    29401 gacttggacg atgcagtgca tatcaaggct ctgaaaaatg gcaatctgga gcttggggtt
    29461 cacatcgcag atgtttctta ttatgtgacc gaggggtctg cccttgacaa ggaagccctt
    29521 aaccgtgcga cttctgttta cgtgacagac cgagtggtgc caatgcttcc agaacgacta
    29581 tcaaatggca tctgctctct caatccccaa gttgaccgcc tgacccagtc tgctattatg
    29641 gagattgata aacatggtcg tgtggtcaac tataccatta cacaaacagt tatcaagacc
    29701 agttttcgta tgacctatag cgatgtcaat gatatcctag ctggcgatga agaaaagaga
    29761 aaagaatatc ataaaattgt atcaagtatc gaactcatgg ccaagcttca tgaaacttta
    29821 gaaaacatgc gtgtgaaacg tggagctctc aattttgata ccaatgaagc gaagatttta
    29881 gtggataaac aaggtaagcc tgttgatatc gttcttcggc agcgtggtat tgccgagcgg
    29941 atgattgagt cttttatgtt gatggctaat gaaacagttg ccgaacattt cagcaagttg
    30001 gatttgcctt ttatctatcg aattcacgag gagcctaagg ctgaaaaggt tcagaagttt
    30061 attgattatg cttcgagttt tggcttgcgc atttatggaa ctgccagtga gattagtcag
    30121 gaggcacttc aagacatcat gcgtgctgtt gagggagaac cttatgcaga tgtattgtcc
    30181 atgatgcttc ttcgctctat gcagcaggct cgttattcgg agcacaatca cggccactat
    30241 ggactagctg ctaactatta tactcacttt accagtccaa ttcgtcgtta tccagacctt
    30301 cttgttcacc gtatgattcg ggattacggc cgttctaagg aaatagcaga gcattttgaa
    30361 caagtgattc cagagattgc gacccagtct tccaaccgtg aacgtcgtgc catagaagct
    30421 gagcgtgaag tcgaagccat gaaaaaggct gagtatatgg aagaatacgt gggtgaagag
    30481 tatgatgcag ttgtatcaag tattgtcaaa ttcggtctct ttgtcgaatt gccaaacaca
    30541 gttgaaggct tgattcacat cactaatcta cctgaatttt atcatttcaa tgagcgtgat
    30601 ttgactcttc gtggagaaaa atcaggtatc actttccgag tgggtcagca gatccgtatc
    30661 cgtgttgaaa gagcggataa aatgactgga gagattgatt tttcattcgt acctagtgag
    30721 tttgatgtga ttgaaaaagg cttgaaacag tctagtcata gtggcagagg gcgtggttca
    30781 aatcgtcgtt cggataagaa ggaagacaag agaaaatcag gacgctcaaa tgataagcgt
    30841 aagcattcac aaaaagacaa gaagaaaaaa ggaaagaaac ctttttacaa ggaagtagct
    30901 aagaaaggag ccaagcatgg caaagggcga gggaaaggtc gtcgcacaaa ataaaaaggc
    30961 acgccacgac tatacaatcg tagatacgct agaggcaggg atggtcctga ctggaactga
    31021 aatcaagagt gtacgagctg ctcgaattaa tctcaaggat ggctttgctc aagtgaaaaa
    31081 tggagaagtt tggctgagca atgttcatat cgcgccttac gaagagggca atatctggaa
    31141 ccaggaacca gaacgtcgtc gtaaactcct gctccataaa aagcaaattc aaaaattgga
    31201 acaagagacc aaagggacag gaatgacctt agttcccctt aaggtctata taaaagatgg
    31261 ctacgctaag cttcttttag gacttgccaa agggaagcat gactatgaca aacgggagtc
    31321 tatcaaacgt cgtgagcaaa atcgagatat cgcgcgtgtg atgaaagctg ttaatcagcg
    31381 ataaaaagag gaattgaaaa tggaaaaatt agttgcctat aaacgcatgc ctttgtggaa
    31441 taaacaaaca atgcctgaag ctgttcagca aaagcacaat acaaaagttg ggacttgggg
    31501 gaaaattact gtcttgaagg gagctctcaa gtttattgaa ttgacagaag aaggggaagt
    31561 tctagctgaa cacctctttg aagcaggggc agacaatcca atggcccaac ctcaagcctg
    31621 gcaccgagtg gaagctgcca cagatgatgt ggaatggtac ttggaatttt attgtaaacc
    31681 tgaggattat tttgctaaaa aatacaatac caatcctgtt cattcagagg tcctagaggc
    31741 catgcagaca gtgaaacaag ggaaagcttt ggatttgggt tgtggtcagg ggcgtaattc
    31801 tctttttcta gcccagcaag attttgatgt gacggctgta gatcaaaatg gactagctct
    31861 tgaaatcttg caaagcattg tggagcagga agatttggac atgcctgttg gcctttacga
    31921 tatcaattca gctagcattg aacaagaata tgattttatc gtttcaacag ttgttctcat
    31981 gtttctacaa gcggaccgca ttccagctat tattcaaaat atgcaggaga aaaccagtgt
    32041 tggtggttac aaccttatcg tttgtgccat ggacacggag gattatcctt gctcggttaa
    32101 tttcccattc acctttaaag aaggagaact ggcagactat tacaaggatt gggaattggt
    32161 taagtacaat gaaaatccag gccatttgca ccgtcgcgat gagaatggca atcgtattca
    32221 actacgcttt gcgaccttac tagctaagaa aatcaagtaa acacacatga agattaggaa
    32281 ttttcctgat cttttttctt ttttacgaat gatatagaaa aggagggaat tcatgtttgt
    32341 tgcgagagat gctaggggag aattggtaaa tgtgttagag gataaacttg agaagcaagc
    32401 atacacctgc ccagcttgtg gaggccagct ccatttgcgt caaggaccaa gtgtacggac
    32461 gcattttgcc cataaatcct taaaagactg tgattttttc tttgaaaatg aaagtccaga
    32521 acacctggcc aataaggaat ccctctatca ctggttgaaa aaagagacaa aggttcaatt
    32581 agagtacccg ctttcagaac ttaaacagat tgcggatgta tttgtaaatg gcaatctagc
    32641 tctagaagtt cagtgtagtc ccttgcctca gaaagtcctt aaagagcgaa gtgagggata
    32701 tcgtagtcag ggttaccaag tactgtggtt gctgggtcaa aaactgtggc tcaaggagcg
    32761 tttgactcgt ctacagcaag gttttcttta tttcagtcaa aacatgggct tttatgtttg
    32821 ggaattagac aaggaaaaac aagttttaag actcaaatac ctgatttacc aggatctccg
    32881 cggtaaactc cattatcaaa tcaaggaatt ttcctatggt caaggtagtt tattggaaat
    32941 attgcgtctt ccctataaga aacaaaaaat atctcatttt acagtttctg aggacaagga
    33001 catctgtcgc tatatccggc aacaacttta ttatcaaaat ctcttttgga tgaaagaaca
    33061 agcagaagcc tatcaaaagg gagaaaatat cctgacttat ggactgaaag aatggtatcc
    33121 acaaattcga ccaatagtgg gcaaattttt ccagattgaa caagacttga ctagctatta
    33181 tcagcacttt tatacctatt accaaaaaaa tcctcaaaat gattggcaaa agctttatcc
    33241 accagccttt tatcagcaat atttcttgaa aaatatggta gaatagaaag gatggaggaa
    33301 tctaatggta ttacaaagaa atgaaataaa tgaaaaagat acatgggatc tatcaacgat
    33361 ctacccaact gaccaggctt gggaagaagt cttaaaagat ttaacagaac aattggagac
    33421 agtagcccag tatgaaggcc atctcttgga tagtgcggat aacctactag aaatcactga
    33481 attttctctt gaaatggaac gccagatgga gaagctttac gtttatgctc atatgaagaa
    33541 tgaccaggat acacgtgaag ctaagtatca agagtactat gccaaggcca tgacattcta
    33601 cagccagtta gaccaagcct tttcattcta tgagcctgaa tttatggaga ttagcgaaaa
    33661 gcagtatgct gactttttag aagctcaacc aaagctgcag gtttatcaac actattttga
    33721 caagctcttg caaggcaagg atcacgttct ctcacaacgt gaagaagaat tattggctgg
    33781 agctggagaa atctttggtt cagcaagtga aaccttcgct atcttggaca atgcggatat
    33841 tgtgttccct tatgtcctag acgatgatgg taaagaagtt cagctatctc atgggattta
    33901 cacacgtttg atggagtcta aaaaacgtga ggttcgccgt ggtgcctatc aagctcttta
    33961 tgcgacttac gaacaattcc aacacaccta tgccaaaacc ttgcaaacca atgttaaggt
    34021 gcaaaattac cgtgctaaag ttcgtaacta caagagtgct cgtcatgcag ccctcgcagc
    34081 gaattttgtt ccagaaagtg tttatgacaa tttggtagca gcagttcgca agcatttgcc
    34141 actcttacat cgctatcttg agcttcgttc aaaaatcttg gggatttcag atctcaagat
    34201 gtacgatgtc tacacaccgc tttcatctgt tgaatacagt tttacctacc aagaagcctt
    34261 gaaaaaagca gaagatgctt tggcagtctt gggtgaggat tacttgagcc gtgttaaacg
    34321 tgccttcagc gagcgttgga ttgatgttta cgaaaatcaa ggcaagcgtt caggtgccta
    34381 ctctggtggt tcttacgata ccaatgcctt tatgcttctc aactggcagg acaatctgga
    34441 caatctcttt actcttgttc atgaaacagg tcatagtatg cattcaagct atactcgtga
    34501 aactcagcct tatgtttacg gagattactc tatctttttg gctgagattg cctcaactac
    34561 caatgaaaat atcttgacgg agaaattatt ggaagaagtg gaagacgacg caacacgctt
    34621 tgctattctc aataacttcc tagatggttt ccgtggaaca gttttccgcc aaactcaatt
    34681 tgctgagttt gaacacgcca ttcaccaagc agatcaaaat ggggaggtct tgacaagcga
    34741 tttcctaaat aaactctacg cagacttgaa ccaagagtat tatggtttga gtaaggaaga
    34801 caatcctgaa atccaatacg agtgggctcg cattccacac ttctactata actactatgt
    34861 atatcaatat tcaactggct ttgcggccgc ctcagccttg gctgaaaaaa ttgtccatgg
    34921 tagtcaagaa gaccgtgacc gctatatcga ctacctcaag gcaggtaagt cggactatcc
    34981 acttaatgtc atgagaaaag ctggtgttga tatggagaag gaagactacc tcaacgatgc
    35041 ctttgcagtc tttgaacgcc gtttaaatga gtttgaagcc cttgttgaaa aattaggatt
    35101 ggcataaaat ggttgaatcg tatagtaaga atgctaacca taacatgcgt cgtcctgtcg
    35161 tcaaagaaga aattgtagac ttgatgcgtc agcgtcaaaa gcaggtcaca ggtttcttga
    35221 aagaattgga aaactttgcc cgcaaggaaa atattcctat tattccccat gaaacggttg
    35281 cttatttccg ttttcttatg gaaaccatgc agcctaaaaa tattctggaa attgggacgg
    35341 ctatcggttt ttcagctctc ttgatggctg aacatgcgcc aaatgctaag attacaacta
    35401 ttgatcgtaa tccagaaatg attggttttg ccaaggaaaa ttttgcccag tttgacagtc
    35461 gcaagcaaat cactctccta gagggagatg cggtggatgt cttatctaca ctgacagagt
    35521 cttatgattt cgtctttatg gattctgcca agtctaaata catcgtcttt ctgccagaaa
    35581 tcctcaaaca tttggaagtt ggtggtgtgg ttgtcttgga tgatattttt caaggtggtg
    35641 atgttgccaa ggatattatg gaagtccgtc gtggtcagcg aaccatttat cgaggccttc
    35701 aaaaattatt tgatgcaacc ttagacaatc cagaactcac cgcaacatta gtgcctttag
    35761 gagatggtat tctcatgctt cgtaaaaatg tagcagatgt tcaactgtct gaaagcgaat
    35821 gattttcaga aaaatttaag aaaaaatagt aaaatagata gagtaacact tatctcaaag
    35881 gagtagacat gaagaaaaaa ttattggcag gtgccatcac actattatca gtagcaactt
    35941 tagcagcttg ttcgaaaggg tcagaagggg cagaccttat cagcatgaaa ggggatgtca
    36001 tcacagaaca tcaattttat gagcaagtga aaaacaaccc ttcagcccaa caagtcttgt
    36061 taaatatgac catccaaaaa gtttttaaaa aacaatatgg ctcagagctt gatgataaag
    36121 aggttgatga tactattgcc gaagaaaaaa aacaatatgg cgaaaactac caacgtgtct
    36181 tgtcacaagc aggtatgact cttgaaacac gtaaagctca aattcgtaca agtaaattag
    36241 ttgagttggc agttaagaag gtagcagaag ctgaattgac agatgaagcc tataagaaag
    36301 cctttgatga gtacactcca gatgtaacgg ctcaaatcat ccgtcttaat aatgaagata
    36361 aggccaaaga agttctcgaa aaagccaagg cagaaggtgc tgattttgct caattagcca
    36421 aagataattc aactgatgaa aaaacaaaag aaaatggtgg agaaattacc tttgattctg
    36481 cttcaacaga agtacctgag caagtcaaaa aagccgcttt cgctttagat gtggatggtg
    36541 tttctgatgt gattacagca actggcacac aagcctacag tagccaatat tacattgtaa
    36601 aactcactaa gaaaacagaa aaatcatcta atattgatga ctacaaagaa aaattaaaaa
    36661 ctgttatctt gactcaaaaa caaaatgatt caacatttgt tcaaagcatt atcggaaaag
    36721 aattgcaagc agccaatatc aaggttaagg accaagcctt ccaaaatatc tttacccaat
    36781 atatcggtgg tggagattca agctcaagca gtagtacatc aaacgaatag tccaaatcaa
    36841 tgagtcaggg aaaaaaactc gacttcagga aaaaatgaag caaacattcc cacaataaaa
    36901 cgcatagtac aaggtttgta ctgaccccaa aagttagaca attaatttat ctgaaggatt
    36961 tagttctgta ttgcacaggg ctaagtcctt ttagttt
//
LOCUS       DAVLYR010000005        36697 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLYR010000005 DAVLYR010000000
VERSION     DAVLYR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.005"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(507..1355)
                     /locus_tag="VYO63_000254"
     CDS             complement(507..1355)
                     /locus_tag="VYO63_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1671241.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1507..1785
                     /locus_tag="VYO63_000255"
     CDS             1507..1785
                     /locus_tag="VYO63_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671242.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1787..3850
                     /locus_tag="VYO63_000256"
     CDS             1787..3850
                     /locus_tag="VYO63_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1671243.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3928..5184)
                     /locus_tag="VYO63_000257"
     CDS             complement(3928..5184)
                     /locus_tag="VYO63_000257"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1671244.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5326..7791
                     /locus_tag="VYO63_000258"
     CDS             5326..7791
                     /locus_tag="VYO63_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1671245.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7856..8767
                     /locus_tag="VYO63_000259"
     CDS             7856..8767
                     /locus_tag="VYO63_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1671246.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8997..9695
                     /gene="dapD"
                     /locus_tag="VYO63_000260"
     CDS             8997..9695
                     /gene="dapD"
                     /locus_tag="VYO63_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1671247.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9763..10893
                     /locus_tag="VYO63_000261"
     CDS             9763..10893
                     /locus_tag="VYO63_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1671248.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10905..11444
                     /locus_tag="VYO63_000262"
     CDS             10905..11444
                     /locus_tag="VYO63_000262"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1671249.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11428..12105
                     /locus_tag="VYO63_000263"
     CDS             11428..12105
                     /locus_tag="VYO63_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1671250.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12177..13076)
                     /gene="galU"
                     /locus_tag="VYO63_000264"
     CDS             complement(12177..13076)
                     /gene="galU"
                     /locus_tag="VYO63_000264"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1671251.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(13098..14114)
                     /locus_tag="VYO63_000265"
     CDS             complement(13098..14114)
                     /locus_tag="VYO63_000265"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1671252.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14336..14739
                     /locus_tag="VYO63_000266"
                     /pseudo
     CDS             14336..14739
                     /locus_tag="VYO63_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(14854..15279)
                     /locus_tag="VYO63_000267"
     CDS             complement(14854..15279)
                     /locus_tag="VYO63_000267"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1671253.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(15347..15997)
                     /gene="phoU"
                     /locus_tag="VYO63_000268"
     CDS             complement(15347..15997)
                     /gene="phoU"
                     /locus_tag="VYO63_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1671254.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(16012..16764)
                     /gene="pstB"
                     /locus_tag="VYO63_000269"
     CDS             complement(16012..16764)
                     /gene="pstB"
                     /locus_tag="VYO63_000269"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1671255.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16766..17581)
                     /gene="pstA"
                     /locus_tag="VYO63_000270"
     CDS             complement(16766..17581)
                     /gene="pstA"
                     /locus_tag="VYO63_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1671256.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17574..18437)
                     /gene="pstC"
                     /locus_tag="VYO63_000271"
     CDS             complement(17574..18437)
                     /gene="pstC"
                     /locus_tag="VYO63_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1671257.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18555..19430)
                     /locus_tag="VYO63_000272"
     CDS             complement(18555..19430)
                     /locus_tag="VYO63_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV1671258.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGKYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19597..20928)
                     /locus_tag="VYO63_000273"
     CDS             complement(19597..20928)
                     /locus_tag="VYO63_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1671259.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMVLTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20929..21636)
                     /locus_tag="VYO63_000274"
     CDS             complement(20929..21636)
                     /locus_tag="VYO63_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1671260.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21720..22022)
                     /locus_tag="VYO63_000275"
     CDS             complement(21720..22022)
                     /locus_tag="VYO63_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1671261.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(22009..22230)
                     /locus_tag="VYO63_000276"
     CDS             complement(22009..22230)
                     /locus_tag="VYO63_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1671262.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            22192..22750
                     /locus_tag="VYO63_000277"
                     /pseudo
     CDS             22192..22750
                     /locus_tag="VYO63_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23065..24756)
                     /locus_tag="VYO63_000278"
     CDS             complement(23065..24756)
                     /locus_tag="VYO63_000278"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1671263.1"
                     /translation="MNTKELIASELSSIIDSLDQEAVLKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            24892..25338
                     /gene="argR"
                     /locus_tag="VYO63_000279"
     CDS             24892..25338
                     /gene="argR"
                     /locus_tag="VYO63_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1671264.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            25389..27923
                     /gene="mutS"
                     /locus_tag="VYO63_000280"
     CDS             25389..27923
                     /gene="mutS"
                     /locus_tag="VYO63_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1671265.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            28070..29764
                     /gene="patA"
                     /locus_tag="VYO63_000281"
     CDS             28070..29764
                     /gene="patA"
                     /locus_tag="VYO63_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1671266.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(30095..30364)
                     /locus_tag="VYO63_000282"
                     /pseudo
     CDS             complement(30095..30364)
                     /locus_tag="VYO63_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            30556..32322
                     /gene="patB"
                     /locus_tag="VYO63_000283"
     CDS             30556..32322
                     /gene="patB"
                     /locus_tag="VYO63_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1671267.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAHQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPIMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            32461..33150
                     /locus_tag="VYO63_000284"
     CDS             32461..33150
                     /locus_tag="VYO63_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1671268.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            33154..33333
                     /locus_tag="VYO63_000285"
     CDS             33154..33333
                     /locus_tag="VYO63_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671269.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            33320..34669
                     /locus_tag="VYO63_000286"
     CDS             33320..34669
                     /locus_tag="VYO63_000286"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1671270.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            34793..36253
                     /locus_tag="VYO63_000287"
     CDS             34793..36253
                     /locus_tag="VYO63_000287"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1671271.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            36267..>36697
                     /locus_tag="VYO63_000288"
     CDS             36267..>36697
                     /locus_tag="VYO63_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1671272.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        10672 a         7030 c         8064 g        10931 t
ORIGIN      
        1 ttattttgga ttttatttgt tattttgacc tgcctctata ttggtaacca tgatttgttt
       61 actctcaatc atcaagaatt ctcttttcgt ggtagctttt ggggtttggt actggcctta
      121 tatcacttat tgttcattga taagtttgta atttcgaatc gaaaataaag attagaacta
      181 tgattgactg gctactttta atattgattt tggaggaaga ttttgtctct attatttatt
      241 attttaaatt tatctatttt gtacaatatc tattctttga gacgggagaa atcgaaatac
      301 ttgatttata cggcctatat catatttggg gtaaatgtag tatatggtat tcaatggtta
      361 ttaagagaac tgatttcaac tatttcccct taatgtgtat ggaaactata acaaaacgca
      421 taagattaag gtttgtgtac ctgttcttat gcgtttttgt aatattgaag attttcggta
      481 agttttatac ctttgggaag tctagtttag aatgctttcc caaccagaat ctctgcttcg
      541 atggtaatct ctgtcagttg gctccagtca atcttgctct ggtcaacatg aaagaggaga
      601 ggagtcatac tgagtagggc ttggagttgt tctgctgtga tagtcttagt cagagaggca
      661 gtttgactag ataggatggt aaagtgttcc tggaaatgct ccttgatatc ttggttagaa
      721 tactccttgt ttgtcagctg gtcctgtact ctttgacgga tttctttgag gtgattttca
      781 gttgggataa cctttatcaa gataccgtct ttggataaaa cgcgacgaaa ttctccatag
      841 ttggcaggtg agaagatatc aagcagaata tccatgttag cgtcttttat aggaagtcgt
      901 gccaagtcgc caacgaacca attgactgcc cagttgggtt cacttttagc agcgatttgg
      961 actgaatctt tggagatgtc aaaggcatag aaagtttttt cagagtgact ttcttgtagt
     1021 ttgcgagaat agaatccttc accacaaccg atatccaaaa ttgttgtggt agtttttgag
     1081 cttgcaagca agtcagatac agcatctaag atagcttggt aaaagccggc ttctaggatt
     1141 tgttgacggt tttgaaaatt ttccttgtca tagttagcag attgcttgat ttgaggtgct
     1201 agattgacat agccaaattt cgccaagtca aaagaatgac ggttgcagca cttgaaatta
     1261 gtctctaaca gagtcagatt ttcttgacag ataggacagg caaaggcagt cgcagaagca
     1321 aaacgctgaa gtttgggctt gagatttgta ttcatagttt aagtgtatca aaagaggcaa
     1381 atgaaagcaa ctttaggaat aagtagatgg gagattcagt agaaataaaa ctaattctcc
     1441 aatttataga atgaaattgc ttttatatct aaattgctat ataataatga taaggaggaa
     1501 ataagtatgt taaaagaagt attaaccgtt gcaaaagttg cgaaaaaatc ttcattattt
     1561 ttgggtggtg tcgcatttgg tacccttggt ttgaaaatct tagcaagtaa ggaagctaaa
     1621 aaaggttatt ctaaagcttt ggctaaggct tacaagttga aagacgagct agatgcatct
     1681 gtttctgttg tgaagcaaca tggagacgat gtcttgcaag atgccaaata tttgtacgag
     1741 caagagaaaa aagaagagca attagatagc cttataggtg aataatatgt cttttaaagt
     1801 gctacataga ggataccaac atatccgact atcatcttct ttttcactta ccttggatat
     1861 tcaagactat cttcgttcct tggcgagaga tgaaaagggg attgagtcta tccagtttta
     1921 catggatcaa cagcacttta ctctacgcat aaaagaaggc ttttctgtat tagataatgc
     1981 agaagccttt ttaaaaagaa ttgataaagg gaaagtttct gagttgatga ctcttcccat
     2041 tcgtagagaa gagagtgctt attctattgt ttcaggtgca gcggttaagc gtgtactttt
     2101 tcgtagtttt gtgccgtatc ctattcgcta tatatggact tgttatcagg ctttgggtta
     2161 tattagagaa gcctatcaaa cactagcgcg taaggaacta acgatggaag tcttggactg
     2221 ttcggctatt ttattgtctt tgtttatgaa ccaatccaag acagctagca atatcatgtt
     2281 tatgcttgat ttggggaatc atttagatca gtggtccttg aaaaaaactg caacagattt
     2341 agaacagagt cttcttgcaa aagagagcga tgtattccta gtacagggcg atacggttgt
     2401 tagtatcaag agttccgatg ttcaaatagg agatgtcttg atcttatctc aaggaaatga
     2461 aattctgttt gatggacaag tagtttcagg tttaggtatg gtcaacgaaa gttccttgac
     2521 aggagagagt tttccagttg aaaaaagaga gtctgatttg gtttgtgcaa atacagtatt
     2581 agaaactgga gagttacgca ttcgtgtaac agataatcag atgaacagcc gtattttaca
     2641 gctgattaag ttgatgaaga aatctgaaga aaacaagaaa acgaaacaac gctatttcat
     2701 caagatggcg gataaagtcg ttaaatataa tttcttgggg gctgggctga cttacctatt
     2761 gacaggttct ttttctaagg ctatttcttt cctattggtc gatttctcct gcgctttgaa
     2821 aatctctact cctgtagctt atttgacagt tatcaaggaa gggttgaacc gtgaaatggt
     2881 gattaaggat ggagatgttc tggagaaata tctggaagtt gatactttct tgtttgataa
     2941 gacaggaaca atcacaacta gttatcctat agttgaaaag gtgttacctt ttggagacta
     3001 tagtgaggaa gatattctca gaatcagtgc ctgtcttgag gaacacattt atcatcctat
     3061 tgctaatgcc atcgtcaagc aagctgagat agagggaatt gaacatgagg aaatgcatgg
     3121 gaaactccaa tatatcgcaa gcaaggggat caaatctcat atagatggcc aaccagttct
     3181 tattgggaat tatgtcttga tgcaggatga gcagattcat atcagttcag aacaaaatgc
     3241 tttaattgaa gagtacaaga gtcactacaa tctcttattc ttggcttatc aaaatgaatt
     3301 gattggaatg ttctgcattc atactccttt gagaaaagaa gcaaaaacag ccttggataa
     3361 acttaaggca caggggaaaa aattgattct ggcaacagga gacaccttga ttaggacaga
     3421 ggaattagtc aaagatttgc cctttgatca ggtctataca gacttgaaac ctgatgggaa
     3481 atttgagtta gtagagaaac tgcagaaagc aggtcacact attttgatgg ttggagatgg
     3541 attgaatgac tcagcggctt taactctatc agatatcggt gtggtgatga atgagagtgc
     3601 agatatttct aagcagatga gtgatatctt attgttagat aatcgtttgg atttcttcca
     3661 agagttggat tcgttatcat catctttgca aacactcatc aagaaaaata ttcaagatac
     3721 cgttgtcgta aatagtagtt tgattggctt tggcttattt aattggctca gtccttcaaa
     3781 tctctctatc ttacataatc taacaacctt gcgcattgtc ctgcgtagcc tgtctattaa
     3841 aaatagatag atgagagtta ttaacagcaa aaaggagtta cctttctcga ggttaactcc
     3901 tttgtttata gataaatgtt gaatggttta gtaagtcaat acaaagtatt ttttcttccc
     3961 acgacggata acagtcagtt cattttctaa cttatcagcg tcactcaaga catagtcaag
     4021 gtcttggatg cggtcgccgt ttacgtagat ggctccgttt tggacgtctt cacgggcttg
     4081 gcgttttgag ttaactatac cagatgagac gagcagttcc acgatattgt tgttttcgtc
     4141 tgcctgtact tggtagtttg gcacaccacg aagtccttgt ttgagctctt tgacagaaag
     4201 gtttttgatg tttcctgcaa agagttgctc agtgatgtta agtgcttctt tgtaggcttc
     4261 ttctccgtga acaagtgtaa caacttcacg agccaagact ttttgagcca agcgttcgtg
     4321 tggcgctgct tcaaattgtt tacgaatatc ttcaatctca tcaagtgaca agaaagtaaa
     4381 gattttcaag aagcgaacag cgtcagcgtc catcacgttc atccagaatt ggtacatttc
     4441 gtatggagaa gtcttttcgg gattgagcca gacggcattt ccttctgatt taccaaattt
     4501 tttaccagtt gcatctgtga ttagtggaac agtgataacg tgaccagtct tgtccgcctt
     4561 acgacgaagc aattcggtac cagctgtcat atttccccac tggtcagaac caccgatttg
     4621 aagagtgaca ttatggtctt ggttaaggac gaagaagtcg tacccttgca tgatttggta
     4681 agcgaactca gtgtaagaaa ttcctgtttc gatccgtttt ttaacagatt ccttactcat
     4741 catgtagttg accgtgaagt attttccaat gtcacggagg aagtcaatga agctgatgct
     4801 gccaaaccag tcgtagttgt tgaccatgac agccttgttt tcgccatttt caaagtcaag
     4861 aaaacgagaa agttgtcctt ggatagactt gacccagcca tctactgtgt cttttgtttg
     4921 gagactacgt tcagcatctt tgaaggacgg atctccgatg agacctgtag caccgccaac
     4981 gagcgcataa ggtttgtgac ctgctagttg caagcgacga cttgtcaaga ttgcgacaag
     5041 gtggcctagg tgaaggctgt cagcagttgg atcgtagcca gtataataag aaacttgacc
     5101 ttcttctagg gctttacgca aagcttcttc atcagtcgtt tgaaatatca aaccacgctc
     5161 ttttagctca tcaaaaatgt gcatgtgtct tttctccttt ttaaaattat tgtttctacc
     5221 tattgtatca caaacttggg caatagccta gcagaatagg gaagaaagtg gctattttat
     5281 tgaaaatttc ccttttgtgg tataatagat agagtgagga aatccatgca aaatcaatta
     5341 aatgaattaa aacgaaaaat gctggaattt ttccagcaaa aacaaaaaaa taaaaaatca
     5401 gctagacctg gcaagaaagg ttcaagtacc aaaaaatcta aaaccttaga taagtcagcc
     5461 attttcccag ctattttact gagtataaaa gccttattta acttactctt tgtactcggt
     5521 tttctaggag gaatgttggg agttgggatt gctttggggt acggagtggc cttatttgac
     5581 aaggttcggg tgcctcagac agaagaattg gtgaatcagg tcaaggacat ctcttctatt
     5641 tcagagatta cctattcgga cgggacggtg attgcttcca tagagagtga tttgttgcgc
     5701 acttctatct catctgagca aatttcggaa aatctgaaga aggctatcat tgcgacagaa
     5761 gatgaacact ttaaagaaca taagggtgta gtacccaagg cggtgattcg tgcgaccttg
     5821 gggaaatttg taggtttggg ttcctctagt gggggttcaa ccttgaccca gcaactaatt
     5881 aaacagcagg ttgttgggga tgcgccgacc ttggctcgta aggcggcaga gattgtggat
     5941 gctcttgcct tggaacgcgc catgaataaa gatgagattt taacgaccta tctcaatgtg
     6001 gctccctttg gccgaaataa taagggacag aatattgcag gggctcggca agcagctgag
     6061 ggaattttcg gtgtagatgc cagtcagttg actgttcctc aagcagcatt tttagcagga
     6121 ctcccacaga gtcccattac ttactctcct tatgaaaata ctggggagtt gaagagtgat
     6181 gaagacctag aaattggctt aagacgggct aaggcagttc tttacagtat gtatcgtaca
     6241 ggtgcattaa gcaaagacga gtattctcag tacaaggatt atgaccttaa acaggacttt
     6301 ttaccatcgg gcacggttac aggaatttca cgagactatt tatactttac aactttggca
     6361 gaagctcaag aacgtatgta tgactatcta gctcagagag acaatgtctc cgctaaggag
     6421 ttgaaaaatg aggcaactca gaagttttat cgagatttgg cagccaagga aattgaaaat
     6481 ggtggttata agattactac taccatagat cagaaaattc attctgccat gcaaagtgcg
     6541 gttgctgatt atggctatct tttagacgat ggaacaggtc gtgtagaagt agggaatgtc
     6601 ttgatggaca accaaacagg tgctattcta ggctttgtag gtggtcgtaa ttatcaagaa
     6661 aatcaaaata atcatgcctt tgataccaaa cgttcgccag cttctactac caagcccttg
     6721 ctggcctacg gtattgctat tgaccagggc ttgatgggaa gtgaaacgat tctatctaac
     6781 tatccaacaa actttgctaa tggcaatccg attatgtatg ctaatagcaa gggaacagga
     6841 atgatgacct tgggagaagc tctgaactat tcatggaata tccctgctta ctggacctat
     6901 cgtatgctcc gtgaaaaggg tgttgatgtc aagggttata tggaaaagat gggttacgag
     6961 attcctgagt acggtattga gagcttgcca atgggtggtg gtattgaagt cacagttgcc
     7021 cagcatacca atggctatca gaccttagct aataatggag tttatcatca gaagcatgtg
     7081 atttcaaaga ttgaagcagc agatggtaga gtggtgtatg agtatcagga taaaccggtt
     7141 caagtctatt caaaagctac tgcgacgatt atgcagggat tgctacgaga agttctatcc
     7201 tctcgtgtga caacaacctt caagtctaac ctgacttctt taaatcctac tctggctaat
     7261 gcagattgga ttgggaagac tggtacaacc aaccaagacg aaaatatgtg gctcatgctt
     7321 tcgacaccta gattaaccct aggtggctgg attgggcatg atgataatca ttcattgtca
     7381 cgtagagcag gttattctaa taactctaat tacatggctc atctggtaaa tgcgattcag
     7441 caagcttccc caagcatttg ggggaacgag cgctttgctt tagatcctag tgtagtgaaa
     7501 tcggaagtct tgaaatcaac aggtcaaaaa ccagggaagg tttctgttga aggaaaagaa
     7561 gtagaggtca caggttcgac tgttaccagc tattgggcta ataagtcagg agcgccagcg
     7621 acaagttatc gctttgctat tggcggaagt gatgcggatt atcagaatgc ttggtctagt
     7681 attgtgggga gtctaccaac tccatccagc tccagcagtt caagtagtag ttctagcgat
     7741 agcagtaact caagtactac acgaccttct tcttcaaggg cgagacgata attctctaaa
     7801 tgaagtggcc aatcaatgga ttgccacttt tttcctttcg tgttacaata agggtatgaa
     7861 tcagtatcag aaaaagattg ttaacggaaa aatttattcg ctcctatccg gcttaatatg
     7921 gggaatctgt ggaattttag gagagtactt ctttactcat tatcaggtgt cttcgggctg
     7981 gattacctct atgcgtttga cactggcagg gagtcttgta ctcatttggt ctgcaataca
     8041 attaaaatcg caagtgctag atatttggcg agacaagaaa aattacctgc cctttttagc
     8101 ctatgctatt ttggggattt tttcagttca gtattttttc tatctctgtg tagaatactc
     8161 aaatgctacg acagcaacta ttttacagtt tattagccct gtttttatcc tcttttacaa
     8221 tcgcttggtt tatcaaaaac gagcgtcaaa aagcgctgtt ttctatgttt tggttgccat
     8281 gctgggtgtt tgcttgatgg cgacaaaggg agatctctct cagttatcca tgacgccgct
     8341 agctcttata acaggtttgc tgagtgccat gggtgttatg tttaatgtta tcttgcccca
     8401 accttttgct aagcgttatg gttttgttcc tacggttggg tgggggatga ttttggcagg
     8461 tttgtttagc aatgtcctct cgccggttta tcagctttcc tttactcttg atatttggag
     8521 tatcttgatt tgcctcatta tcgctttctt tggaacggct tttgcttttt tcatttccat
     8581 gaaggctgtg tccttggttt ctcctttggt ggttgccgtt atcagtgcca gtgaacctct
     8641 ctcttctgct ctcttgagtg ttttgttctt aggattagta gtggattggt ccctccttct
     8701 agctatagcc ttgattattt tacccatgat atttttgtct atagaagaag cgaaagaaag
     8761 tagataaaaa gtcttttctt aattctaaaa gtgtgctata ctagaatagt caataaaaca
     8821 cggggagata gttgtgaaga gtgtttttag gttgtatcgg taggggcttg cttttaccga
     8881 cagcacgttt tatctcacat ccctaaaaaa tcatacctaa aaacagacaa aaaggtttca
     8941 aatttgaggc cttttttggt agaataggta tcattataac gaaccaggag gcacctatga
     9001 ctgctacaaa aatgaacgct caagaaatta tccaatttat cgccaatgct gaaaagaaaa
     9061 ccagtgtcaa agtaaccttt gaggggcaac tcgcaactgc tgtgcctagc tctgttgtca
     9121 aactaggaaa tgtcctattc ggagactgga aggacgtggc tccgcttctt gaaggtttgg
     9181 tagaaaatca agattatgtt gtcgagcaag atgctcgtaa ttctgcagtt cctttgctag
     9241 ataagcgtgc tatcaacgct cgtatcgagc caggtgctat tatccgtgac caggtggaaa
     9301 ttggtgacaa tgctgttatc atgatgggag ctgttatcaa tatcggtgct gaaatcggtg
     9361 ctggaaccat gattgacatg ggtgccatcc ttggtggccg tgctatcgtt ggaaaaaata
     9421 gccacgttgg tgcaggtgca gttttggcag gtgtgattga gccagctagt gctgaaccag
     9481 tccgtgtcgg agacaatgtt cttatcggtg ctaatgcagt ggttatcgaa ggagtccaaa
     9541 tcggtagtgg ttcagttgtc gctgcaggag ctatcgttac ccaagatgtc ccagaaaacg
     9601 tggtagtagc aggtgttcca gctcgtatca tcaaagaaat tgatgcccaa acccaacaaa
     9661 aaacagcgct agaggatgcg cttcgtacct tgtaattgta aaagtaaaaa agaggcggaa
     9721 ccctttttcc agcctctttc tgctataacg gaggacagat agatgttaga tttgattcag
     9781 actagacgag ctttacacca gattccagag attggcttgg aggagttcaa gactcaggct
     9841 tatttgctgg atgtgattga gaaattgact acgggcaagg attttgttca aattcgtact
     9901 tggcggacag ggattttggt ctacctgcag ggaagtcagc cggagcgaac cattggttgg
     9961 cggacagata ttgatggtct gcctatcgtc gaacaaacag gtctaccttt tgcttctcaa
    10021 caccaaggtc gcatgcatgc ctgtggccat gattttcata tgaccattgc cttaggctgt
    10081 cttgagcgcg cccttgagga gcaacccaag aataatctgc tctttctatt tcagcctgct
    10141 gaagaaaatg aagctggtgg catgctcatg tatgaggacg gtgcttttgg agattggttg
    10201 ccaaaccaat tctatggtct ccatgttcgt ccagatttga aagtcggcca gattgcgact
    10261 aatactcata cactctttgc aggaacttgc gaggtgaaga tccgtttcaa aggcaaaggt
    10321 ggccacgcag cttttcctca tgaagccaat gacgccttgg tggcggctag ttactttgtg
    10381 acccaagtgc agtcagttgt tagccgcaat gtcaacccaa tcgagggagc agtggtgacc
    10441 ttcggcgttt ttcaagctgg aaccaccaac aatgtcatca cagacacagc ttttttacat
    10501 ggaactattc gtgccttgac acaggacatg agtctcttgg tgcaaaagag ggtaaaaaca
    10561 gttgcagaag gggttgcagc tgcctttgat atggaagtcg aagtggaact caaacaaggt
    10621 ggatacctac ctgttgagaa caatccagcc ttggcgcgtg aactgatgga cttctttgac
    10681 gaaaaagacg gaatcgagtt gattgatatc gaacctgcta tgacaggcga ggactttggt
    10741 tatctccttt caaaggttga tggcgtgatg ttttggctag gtatcgatag tccctacgct
    10801 cttcatcacc ctcaaatgag tcctaaggaa gaagtcttag ctattggggt ggctgcggtg
    10861 tctagtttcc tgaaaaagaa ggcagcagaa tagagggctt gtctatgaaa tcggaattac
    10921 gcaagcaagt cttgcatgaa atgaaggcta tatctcaaga gcaaaaacag gctatagacc
    10981 aagctttaac cgagcggctt ttacaccatc ccttctacca agaagccaag gtcatcgcaa
    11041 cctacctctc tttttctcat gagtttcaaa cgcgggaact gattgagcag gcgctgaagg
    11101 acggcaagaa ggttttaata cccaaaactt atcccaaggg gcgcatggag tttgtggtct
    11161 atgatccgca acagttggta aaaacttcct ttgccttact ggagccacag ggagatgtgg
    11221 aagtggtgga tgcatctcag attgatttga ttcatgttcc tggtctggct tttacgacga
    11281 aaggatatcg gataggatac ggtggaggct attatgaccg ctatctggaa catttttctg
    11341 gtcgtacttt gagtacgata tatccttgtc aaattcagga ctttatacct gaaaaccatg
    11401 atattcctgt tcaggaggta ttaattgatg aaggaaatct ttgatagacg ttaccctgtg
    11461 acgagtttct tcctcttagt gacggccttg gtatttttac taatgttggt cactgcaggc
    11521 ggaaactttg acagggcaga tacattattt cgatttggag ccatgtatgg gccagctatt
    11581 cgcctctttc ccgagcaggt ttggcgtctc ttgtctgcca tttttgttca tattgggtgg
    11641 gaacatttca ttgttaatat gctttcactt tattatcttg gaaggcaggt agaggagatt
    11701 ttcggttcta agcagttttt ctttctctat cttttatcag gaatgatggg caatctcttt
    11761 gtttttgtat ttagtcctaa atccttagca gcaggcgctt ctacctctct ttatgggcta
    11821 tttgccgcga ttattgttct tcgctatgca actcgcaatc cttatattca acagctaggg
    11881 caatcctatc tgacactttt tgtggttaac attattggaa gtgttctgat tccaggaatc
    11941 agcctagcag gccatatcgg tggtgcagtt ggtggcgcat ttctagcagt tatctttcca
    12001 gttagaggag aaaaacggat gtataatacc agccagagat taggagcggt agtcttgttc
    12061 gtaggactcg ccattttgct tttctacaag ggaatgggaa tgtgacgaag ttgaaagaaa
    12121 atttgtgtaa tgaaaaaaga taaggcattt tttgaacctt atctttttat tttattttat
    12181 tccttctcag tcaattcttt tccaagttgg atgaggtaat tcttcaaatc atctttgact
    12241 tgtgggtgtt tgagggcgta gtcgatggat gttttcatga agccaaactt gtctccgaca
    12301 tcgtaacgag ccccattgaa ctcacgagca aatacacgtt gtgttttatt gagggtgtcg
    12361 attgcatctg tcagctgaat ttcatttcct gcacctggag cttggttttc gaggatttgg
    12421 aaaatttcag gcgtgaggag gtagcgtccg ataatagcaa ggtcgctagg agcgtcctct
    12481 ggagctggtt tttcaacaaa ggtttcaaca ctgtaaagac catcttttcc ttcgccttgc
    12541 ggagcaataa ccccgtaagc agatacttcg tcatgaggga ctggcatgac agcgatagta
    12601 gacgcgtggg tacgctcgta gtcatccatg agttgtttgg taagtggaac agccttttcg
    12661 tctgtgatat ccatcaagtc atcaccaagc ataacgacaa aaggttcatt tccgacgaaa
    12721 gccttagctt gcaaaacagc atctccgaga ccgcgtggat gagtttggcg gataaaatgc
    12781 agacgcatgt cagttgtttt atcaactagc ttcaaaagat ctgttttccc tttttctttg
    12841 aggttatatt ccaattcgaa gtttgaatca aagtggtcct caatagaacg ttttgattta
    12901 ccagtgacaa ctagaatatc ttcaatacct gatttgagag cttcttccac gataaactgg
    12961 atagttggtt tgtctacgat tggcaacatt tctttggcaa gggccttggt tgctggtaaa
    13021 aatcgagttc ctagtccagc agcagggatg actgcctttc taacttttga tgtcataaaa
    13081 atcctttcta tagagggtta agaccactca ttttctgctt taaattcatt gttcatgatg
    13141 tcataaatgg catctttgat attggttccg tgataaataa cttggtaaat agcctgtgta
    13201 atgggcatat agactccaag ttcttgggct agttcatagg ctgctcgagt cgttgaaatt
    13261 ccttcgatta ccatgcccat attagcttct atatcagcta gggattctcc tcgtccgaga
    13321 gcatctccag ctctccagtt acgagagtgg atggaagttc ccgttacgat caaatctccc
    13381 acaccagata agccgctata ggtcaatgga ctggccccga gtgctacccc taggcgggtg
    13441 atttctgcta aacctcgagc gatgatggct gccttagcat tatcaccaaa tccaagacca
    13501 tgtaaagctc cagcaccgac agcaataata tttttaagag caccagcagt ttcaaccccg
    13561 ataacatccg tattggtata aagtcggaag tagtgattac taaatagctt ctgaacgtat
    13621 tgagctgttt gtaaatcttt agaagcagca gttattaaag ttaggtcacg cacaatggtc
    13681 tcttctgcat gactaggccc tgaaacaacg acgatatcac tacggagatg ttcaggaatt
    13741 tcttcttcaa gaatggttga taatcgttta tggctatcag gttctaatcc ctttgatgcg
    13801 tgcatgatga taaccttatg gtccaaggtt tgtgcaactt gctgggcaac aagtcgtgtc
    13861 acttttgttg ggacaacaaa caaaatcgca tccacatctt tcaatgtttc tgctaagtcg
    13921 gtgtaggcaa tgatattttc gtctagaacg acatctttaa agtagtgctt attagtatgg
    13981 tgtgtattaa tttcattgat ttgctcggga agatttcccc aaatacgtac ctcgtgtcca
    14041 ttgtcattta agacttgtga aagggcggtt ccccaagaac caggccccaa gacggcgacg
    14101 gtttgttttt ccatgatttc ctccttgata gagttctttc tcttattcta tcatattcta
    14161 ggggattttg cacttgcatt actggggtat ggtggaaaaa tacacaaaaa ccgagacgaa
    14221 gggataaact cttagtcttt agttaaagtg agaagaaatc tgatagatag tttgattcag
    14281 tatatgctta taatagaggt aaacaactca ggaagttctt gtaggttgcg agagtttggc
    14341 taaaaaaatc agtcgttcag aattttgtgg tgatgagtct gaattaagta tccgtcaatt
    14401 aattagaatt gaaaatggag aatccagacc aacactaaca aagttaaaat atatcgctga
    14461 acgtttgggg gttgaagatt acaagttgat gccaagttat atagagttgg ataaggaata
    14521 cctagaattg aagtatttct tgatgaggac tcctacatac gaagatgaaa ctatcgccca
    14581 aaagaaagag agtgttttga taagattttt gaagagtatt atgataggct acctgaggaa
    14641 gaaagattta tcatcccaaa ttattcatat ctggcactaa cgaactacac agttcaaaaa
    14701 ttaccagaaa agctagttga aatactgtcc ttctggtgat tttgcttaat ttctgatgtt
    14761 cgagacataa ttgaggagat ttgtcttgat tttttcctaa aaaagggcag actcctccct
    14821 tggtttgatg ctcgattttt aaaataaggt gacttattct gttaggcatg ttttcccctg
    14881 ccctctagct ctatatggaa cagggagtat cagatttatc tggcagattg tatcaaataa
    14941 ggcttgaaac tgttttgaat aggcatatag acttgagaat ttgagaatat gtcgtctagc
    15001 agtagagaca tattttccgg caatatgaag gaagagacgt cgaaaacgct tgatagtcaa
    15061 ggcctttact tcatcaccag ctagctgctt taaaaagagg tagagattat aagccagaca
    15121 gcccatcatc atacgtactt cattcttaat catggtcgaa ctatctgtct tatccccaaa
    15181 gaatcctgct ttcctttctt tgataaagtt ttcagcattc cctcgttcac gatagaattg
    15241 aaacagctcc tcactatcta cactgcttaa attgttcaaa tgaacttttt tacaaccata
    15301 aaaaagagca cttaatcgta ctcttctctt ttaaggatta gttgaattaa ttcaaatcca
    15361 ccagttctcc tgtttctagg tagactaggc gttcacaaat gttagcaatg taatcagcga
    15421 agcgttccag atgccctatg atataaaggt attgagttcc attgggaatt gaggtttctt
    15481 ggtctttcat aagtccaatg atttcctttg ataaggcata ataatattgg tcaatctgtt
    15541 catctttttg agcaatacta atagcttttg aggcttggtg caaaggaaag gcaaccaata
    15601 aatcggctag catgctgagg gataatttac ccatttggtg taactgttct tcgtcagggg
    15661 ctagttgatt ttcttttagt tgcaaaacag ctttagcaat gcctgccata tggtctccca
    15721 tacgttcaag gtctgaacaa gaagacatga tgctaatcac aaatcgaagg tcagacactt
    15781 gtggctgctg caaggccaac aaacgggcac aggtcaattc gatagcgctt tgaccttggt
    15841 tgatagcatg atccttattg ataattagct ctgccatctc cttgtctttg gaggctaagg
    15901 ccagtaaggc ttttgaagct gtttcaagga caagttgccc tagtcctaaa aaggattgtt
    15961 ctaattcatg caattctaag tcaaattgat ttctcatagg tttttccttc cttatccgaa
    16021 ccgtcctgaa atatagtctt ctgtgcgctg atcttttgga ttggtaaaca cgtcaacggt
    16081 atctccaaat tcgcaaattt ctcctgttaa gaaaaaagca gttttatctg aaatacgtga
    16141 agcttgttgc atgttatggg taacaatgat aatcgtataa tcctttttta gttgctgaat
    16201 gaggtcttca atttttaaag tggagatagg gtctaaggct gaagtcggct catccattaa
    16261 cagaatatca ggttctactg ctaaagctcg cgcaatgcaa aggcgttgct gctgaccgcc
    16321 agataaggac atggcactct ttttaagatc atctttgact tcttcccaaa tggctgcccc
    16381 ttttaaagat ttctccacta aggcatctaa ttgttttttg tctcgaatac catgtgtcct
    16441 tgggccataa gccacgttat catagataga catggcaaag ggattaggct gttgaaaaac
    16501 catccctaca cgcttacgta gctgattaag gttgaatttg ctactataaa tatcttgctc
    16561 atctaagagg acttggcctt caatatggca agaaggaacc aaatcgttca tccggttaag
    16621 ggtttttaga aaagttgatt tgccacaacc agatgggcct atcaaggcag taatctgtct
    16681 ttctggtaat tgaatcgaaa tattttttaa ggcttgaaaa tccccgtaaa ataagtctag
    16741 gtgtctgact gaaaatgttc ccatactagg aagctccttt cacaagtttt cgagataata
    16801 agcttgatag agtatttatc attaaaacag taataatcaa aatcacaccg gtagcatagg
    16861 cttcattgac atgtagcccc tcacttgaca gcatatacat atgcagggct agagaacgac
    16921 ctgaagacat gagactactt ggcgtattgg tagaggtacc taatgtatac atgagggcag
    16981 ctgtttcacc aacgatacgg ccaatagcta gtatcactcc agctaaaata cctggcatgg
    17041 caactggtag aacaattcta aaaacagtcc gtaacttacc tgccccaagt ccataacttg
    17101 cttgacgcat gctatcacta acagataaaa gggcttcttc tgttgagcga ataatgactg
    17161 gcaacaccat gataactgag gttaagattc ctgataacag agagtattga aaacctaaga
    17221 agactacaaa gaagagcatg ccaaacagac caaaaacaat ggaaggaatc ccagacaagg
    17281 tatctgaggc caatcgcatg attttaacac aaagggaatc tttttttgta tattccacaa
    17341 gataaaaacc agcaaaaatc cctatgggca aggctaaaag aagagcacca aagaccagaa
    17401 taacggtgga aataatcgct ggcataaggg aaatgttctc agaagtataa gtccaagaaa
    17461 agagggatag acttagatga ggtaagcctt tgatgaggat aaaaccaatg attaaaaaga
    17521 gagagccaaa ggttaaagct gaaaaacaat aaacgagaag ttttagcagg tatttactca
    17581 taagatgatt ttcctttcaa gtaggcaaag taggcattaa tcaagagaat aaggaaaaag
    17641 agaactgctg aggttgcaat aagggcttcc ctatgctgac ctgatgcgta agccatctcc
    17701 agaacaatat tggttgttaa ggttctggtt cctgaaaaga gtccacttgg aataattggc
    17761 tggttgcctg ccaccaaaat aactgccatg gtttcaccta ctgcgcgacc gattcctaaa
    17821 ataactgctg ataaaatacc agatctcgca gctggcaaga tgacactaaa aatactccgt
    17881 tcatgactag ctcctagagc caagctacca gaataatacg ttttgggaac tgttcggata
    17941 gcagattctg acaaactgat aatggttggc aaaatcatta ttcctaatag taacgaagcg
    18001 gttaggacac tcatgccatt tcctaaaaag cttctaatcc aaggcaccaa taattgtagg
    18061 ccgaaaaaac cataaacaat agatggaatg gctgccatca agttgatagc tgattttaag
    18121 aagccataga cgggctttgg acaataataa accataaaca ccgatgtcaa gatgcctgtt
    18181 ggcaccccaa tcacaatcgc tcctaaggta attaataagg aaccaacgat cattggtaaa
    18241 ataccatagc ttgccggaat gttcgttggc gaccaatcac tgcctaataa aaaacgggca
    18301 aagccgtagt tagctatgaa aggtaagcca ttactaaaaa taaagaaaca gattagcaaa
    18361 atagctacaa cagctactgt tgcactcatg aaaaaaattg ccctaaaaac tgcttctttg
    18421 aaggcttgtt ttgtcacatc ttgtcctttc tagtgaagaa agtaagggag atacgacacc
    18481 tccctacttg ccttctttat cttattgtac gatgaaacgt ctgcatctct ttagagattt
    18541 atggagcaaa cattttattt aatcttgtcc caggtggtta atttgccact aaaaacgtct
    18601 gcaagttcag ccatactgac ttggcttgcc ttattgtcat tattgaccac aacagcaata
    18661 ccgtctaaag caatagcatc atgggtgaga ctcttacctt cttcaggagt taattcccta
    18721 gaaaccatac caatatcagc ggttttctcc ttaacagcgg taatacctgc tgaagaccca
    18781 ttagaggtaa tatcaatcgt aacttctgga ttttcttttt tataagcttc tgctaatttt
    18841 tccattaaag aagatactga agtggaacct acaacagaca acttgcctga taagtgttgg
    18901 cttgtatatt ctgtggtttc ggttttagct tcaataaatt tattatctgt gaccacttgt
    18961 tgaccttgtt tggagtggat aaagctgata aaatcttgac ctagcttgga aagattagaa
    19021 gaccaaacaa tgttgaaggg acgttgaaga gggtatttac catctaaaac tgtgtctcga
    19081 ctagccttga caccatcaat ctctaaagct ttgacagatt tcgttaaaga tcccaaggag
    19141 atgtagccga tagcattagc attcccttga actgctgaga gaacaccttc tgtactattt
    19201 tgaatcacag ctgttttggc agtgttgtca atttttttat caccgtcttt tttgagaatc
    19261 cctgtgattt ctgtgaaggc accccgtgtt ccagagccat tttctcgtga aatcacctca
    19321 atcgttcctg aagctgactg tttggaagca gctgactgat tgccacaggc aacaagccca
    19381 aatcctgata agccaatggc tgcaagagta agcatttttt tgaatttcat aataatcacc
    19441 tttatctcta tgtatttttc ttgtgtaggc ttactacatt tatagtctaa caagtctttg
    19501 taaaggttta tccctgattc atgtaaagat tgtgtaaaga atcaaaaaaa gccacttttg
    19561 aaaaatggct gctcctaaaa atagctttaa aaattattag tcctgtgcga aagattggtt
    19621 aggaagaaaa atcgtgaagc aactgcctct gccaagctga ctcgtcaccg tgacttggcc
    19681 acctaataat tgactgagtt ctttgacaat ggcaaggcca agaccagtgc caccagtttg
    19741 tctgcttcga cctttattaa ctcggtaaaa acgttcaaaa atacgatcct gctctaattg
    19801 actaatacca atccctgtat ctgatacaga aatcttaatg ccttcgttca ccttttgggt
    19861 cttgacctca atttttcccc cttgttcagt gtaacggatg gcattggata aaagattgag
    19921 taagatttgg gaaagtaatt gactatctga tacgagggtg acatcatctg gcacctgcac
    19981 ctttagctgt aaatccttct tcttgagctg aggttgcaag ctttgagtca aatcctgtac
    20041 aaattctgcc aaagaaaggg tcgtccattg tataggcatt tgttgagcct tagataaggt
    20101 aagaagatgc tcaacaatat gctcaagacg caagctttct ttgtaaataa tgtctagaaa
    20161 gtcatccttg agcgcttctt cttcagctga catcccctta atggtttcag caaagccctt
    20221 aatcgaagta actggtgtcc tcaattcatg ggaggcattt gagacaaagg ctaaatttaa
    20281 cttttcataa gttctaatcg ttgttaaatc atatagcaag acgagcacag cttccacaga
    20341 ttgggtgggg ctaaaaacgg gaactgctgt cacttctaaa atcaagtcac cctcatgaaa
    20401 cccacttact tcttgtttta accttgtttt ttgatcaaag gcttggtgaa ctaaatttcg
    20461 aatatccatc cgtttgaggt catcaagtga acttatgtcg ccgtccacat cgggaaaata
    20521 atgaggcaga gagcgactgg ataataacat ctgaccttga gcggaaacta aaaacgtccc
    20581 catggttagg tgcgacagaa gaacctccat tgtttcggct aggtccttgt attgctgatc
    20641 ctgttgggag actttggttt ttagggcaga cacatactga gccaaagact ttaagtcttc
    20701 ttgccctttt tctaaaaagt attcactact ggtcaagaga ggttggtgca aggtctcaaa
    20761 agcaacttcc catttccaaa ggcaaaagag ccagtagcca ccttgtccca aagaaagggc
    20821 tagaagaaag agaccgatgc ctttactgat ccaagttaat accatccctg caatcagaat
    20881 gaggctaaca cttagattga ctagccaaaa ttgaaggtag cgtttcatct ataactcctt
    20941 gaacttataa ccataacccc gaatggttcg aataaattga ggggctttag gattgtcttc
    21001 aattttttcc ctcaacttac caatatgaac gtccaccaaa cgtgtttcct gcccaaagtc
    21061 atacccccag atacgttcca aaagacgctc tctagtcagt gtcatgttgg gatgtttcat
    21121 aagatagagc aagagttcaa attcttttgg ggtcaaactc agtaacttat tcgccttgta
    21181 gacttcatga cgctcagggt atactttcaa ggtcccaaat agccaagaat cgtcagcgat
    21241 attatctgaa tcatctcctt cttgttctcc tttagttcgc ctgaggacag ccttgacacg
    21301 cgccagcaat tctctagggc taaaaggttt ggtcaggtag tcatcagccc ctaattccaa
    21361 ggccaaaacc ttatcaaatt catcactttt cgcagaaacc atcataattg gagttttgac
    21421 gcctttggct ctcagccgct tacaaacttc catgccatct aattgtggta acatgatatc
    21481 aagcaagata aaatcaaagg gttctgtttc tgccaaagct aaggccttcc gtccatttgt
    21541 taccaattga gtagaaaagc cttccttact taaatggtag tcaagcaatt tcagaatgtg
    21601 ttcttcatca tccactaata agacttgttt tgtcatctat tatctcctat tggtaacatt
    21661 ataacacaat tatcagaaat cctaacattg ctaaatcaga ttaaatttgc ttatcaagac
    21721 tagtatctgg tcaaacgctc aatcatctcc ttgtgctctg gataggtcgc cagtagatct
    21781 accctttcaa ataattcaaa atcctcaaat tcaaaaccag gagcaacaag acaagaaacc
    21841 agagcatcat ccttatcaac tgttgatccc caaatagtgc ccttaggaac acagtagtga
    21901 agttgttgcc ctttggatat gtccaggcct aaagtgactg cttcgtagtg accatctgct
    21961 gtaatcatgt gaacagtaag tggggattct gcatgaaaat accagatttc atctgctgtc
    22021 aatcggtgaa aatgtgaagg attcgtttct tcttaataag aaataaatac tggtataaag
    22081 cgcccttccc ttaccagcaa ggtttatagt gtctgaagct ttttttgttt gtctaaaata
    22141 gccaccttca acatggggag ctaactctag agttcttatc aagtcttctt tatccgtcgg
    22201 agccaatggg ttgaagtaac tcttgttcaa agtggtgtta cgatttcaag aactcctctc
    22261 agttctgagg acacggtaat gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa
    22321 agaattagcg aatcattctg gtaaaaagaa atttcacgct atgaaggctc aagcgattgt
    22381 cacaagtcaa gggagaattg tttctttgga tatcgctgtg aactatagtc atgatatgaa
    22441 gttgttcaaa atgagtcgca gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg
    22501 ttatcaaggg ctcatgaaga tatatcctca agcacaaatt ccacgtaaat ccagcaaact
    22561 caagccgcta acagttgaag ataaagcctg taaccatgcg ctatctaagg agagaagcaa
    22621 ggttgagaac atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa
    22681 tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct ggtattatca atcatgaact
    22741 aggattctag ttttgcagga agtctattat ttggttaggt gaattagtga agcgtttagg
    22801 caagtgtctc tggttacgac gtcatggcct ctaaatcgat tatatttagg ggtcatgact
    22861 agtgaagcag ttagctagtt cgcatataag cggctagcgt ctaacaatta ggaactttag
    22921 ttccaataac tttaagatta cgacgtttta ggacataaat cgatcatatt tatgtcctaa
    22981 aactagtgaa gcgcctagcc aaagtccgaa taggatttgg cgttagttac ttagattgct
    23041 ttgcaatcaa gtaactttgg cgatttacat cttctctggt gcttctactc caagcaagcg
    23101 aagggcttct ttgagaacga ctgcggttgc gtagctgagg gctagacggc tgtcgcgttc
    23161 tgggctttca tccaagatac gtgtatgtgc atagtatttg ttaaaggatt gagccaggct
    23221 aattgcaaat ttagcaatga tagaaggttc aaagttatct gccgcacggt tgataatacg
    23281 tgggaagtct tgaatgagtt taatgatttc ccagctttca gtatcattca agctatagtt
    23341 gccagctgtt tctggtttga aatcggcttt gcgtaagata gattggatac gagcgtaggc
    23401 atattgaacg taaggtccag tttcaccctc gaaggatacc atagcttcta ggtcgaagtc
    23461 gtatccattt gtacggtcag ttttgaggtc atagaattta atggctccaa ccccaacagc
    23521 atgtgctact tggtctttgt tttctagttc aggattttta gcctcgattt ggaccttggc
    23581 acggctaaca gcctctgcaa cagtaggctc tagcaagatg acattccctt tacgagtaga
    23641 gagcttcttc ccttcttttg taaccagacc aaaaggaacg tgggtaatgt cgtcactcca
    23701 atcgtagccc atctcttgca agacagcttt gagttgttta aagtgggcag attgctcttg
    23761 accaacgacg tagatagatt tagcaaattg gtattcattt ttacggtaga gggctgcagc
    23821 caagtcacgt gtgatataga gagttgcacc atcagacttc ttgatgaggg ctggatgttc
    23881 aattccatat ttctcaagat tcacaacttg ggcaccttct gattcaagaa gtagtccttt
    23941 ttcagaaaga atgtctacaa ctgcatccat cttatcattg tagaaggctt ctccgttata
    24001 gctgtcaaat tcaaccttca attcattgta aaggcggtta aattccacta aactttcatc
    24061 gcggaaccat tgccaaagag cgagagcttc ctcatctcca ttttcaagtt tacggaacca
    24121 ttcgcgcgct tcttcatcca agctagggtc attttcagct tcagcgttga tgcggacata
    24181 gagtttaagg agttcatcga ttggatgagc ttttacagct tcttcgtcgc cccatttttt
    24241 ataggcaaca atcaacatcc caaattgttt accccagtct cccaaatggt tgaccttgac
    24301 cgtttgataa ccgatttttt ggaaaatatg tgacaagcta tctccgataa cagttgaacg
    24361 caggtggcca atagaaaatg gtttagcgat attcggacta gacatgtcga taacaacatt
    24421 ttcttgttta ccaatatttt ggtcagcata gtgttctttt tcagtggtaa cagcttgcaa
    24481 tacttgagca gaaatggcag atttatcaag gaaaaagtta acgtaaggtc ctgttgcgac
    24541 aactttttca aaggcttggc tgttcatttt ttcagccagt tcagccgcaa tcatttgtgg
    24601 tgctttacgt tcgacttttg caagagaaaa agcagggaaa gcaatgtctc ccatttctga
    24661 gtttttaggg gtttccagta actttaaaac agcctcttgg tccaggctat caatgatgct
    24721 agataattcg ctagcaatca attcttttgt attcattaag agctcctttt tggacttttc
    24781 tactatttta tcacaatttt aaagaaagaa gaaaaaattt ttgaaatctc ctgttttttt
    24841 ggtataatat ggttataaat atagttataa atatgcacgc aagaggattt tatgagaaaa
    24901 agagatcgtc atcagttaat aaaaaaaatg attactgagg agaaattaag tacacaaaaa
    24961 gaaattcaag atcggttgga ggcgcacaat gtttgtgtga cgcagacaac cttgtctcgt
    25021 gatttgcgcg aaatcggctt gaccaaggtc aagaaaaatg atatggtgta ttatgtacta
    25081 gtaaatgaga cagaaaagat tgatttggtg gaatttttgt ctcatcattt agaaggtgtt
    25141 gcaagagcag agtttacctt ggtgcttcat accaaattgg gagaagcctc tgttttggca
    25201 aatattgtag atgtaaacaa ggatgaatgg attttaggaa cagttgctgg tgccaatacc
    25261 ttattggtta tttgtcgaga tcagcacgtt gccaaactca tggaagatcg tttgctagat
    25321 ttgatgaaag ataagtaagg tcttgggagt tgctctcaag acttattttt gaaaaggaga
    25381 gacagaaaat ggcgatagaa aagctatcac ccggcatgca acagtatgtg gatattaaaa
    25441 agcaatatcc agatgctttt ttgctctttc ggatgggtga tttttatgaa ttattttatg
    25501 aggatgcggt caatgctgcg cagattctgg aaatttcctt aacgagtcgc aacaagaatg
    25561 ccgacaatcc gatccctatg gcgggtgttc cctatcattc tgcccaacag tatatcgatg
    25621 tcttgattga gcagggttat aaggtggcta tcgcagagca gatggaagat cctaaacaag
    25681 cagttggggt tgttaaacga gaggttgttc aggtcattac gccagggaca gtggtcgata
    25741 gcagtaagcc ggacagtcag aataattttt tggtttccat agactgcgaa ggcaatcaat
    25801 ttggcctagc ttatatggat ttggtgacgg gtgactttta tgtgacaggt cttttggatt
    25861 tcacgctggt ttgtggggaa atccgtaacc tcaaggctcg agaagtggtg ttgggttatg
    25921 acttgtctga ggaagaagaa caaatcctca gccgccagat gaatctggta ctctcttatg
    25981 aaaaagaaag ctttgaagac cttcatttat tggatttgcg attggcaacg gtggagcaaa
    26041 cggcatctag taagctgctc cagtatgttc atcggactca gatgagggaa ttgaaccacc
    26101 tcaaacctgt tatccgctac gaaattaagg atttcttgca gatggattat gcgaccaagg
    26161 ctagtctgga tttggttgag aatgctcgct caggtaagaa acaaggcagt cttttctggc
    26221 ttttggatga aaccaaaacg gctatgggga tgcgtctctt gcgttcttgg attcatcgcc
    26281 ccttgattga taaggaacga atcgtccaac gtcaagaagt agtgcaggtc tttctcgacc
    26341 atttctttga gcgtagtgac ttgacagaca gtctcaaggg tgtttatgac attgagcgct
    26401 tggctagtcg tgtttctttt ggcaaaacca atccaaagga tctcttgcag ttggcgacta
    26461 ccttgtctag tgtgccacgg attcgtgcga ttttagaagg gatggagcaa cctactctag
    26521 cctatctcat cgcacaactg gatgcaatcc ctgagttgga gagtttgatt agcgcagcga
    26581 ttgctcctga agctcctcat gtgattacag atgggggaat tatccggact ggatttgatg
    26641 agactttaga caagtatcgt tgcgttctca gagaagggac tagctggatt gctgagattg
    26701 aggctaagga gcgagaaaac tctggtatca gcacgctcaa gattgactac aataaaaagg
    26761 atggctacta ttttcatgtg accaattcgc aactaggaaa tgtgccagcc cactttttcc
    26821 gcaaggcgac gctgaaaaac tcagaacgct ttggaaccga agaattagcc cgtatcgagg
    26881 gagatatgct tgaggcgcgt gagaagtcag ccaacctcga atacgaaata tttatgcgca
    26941 ttcgtgaaga ggtcggcaag tacatccagc gtttacaagc tctagcccaa ggaattgcga
    27001 cggttgatgt cttacagagt ctggcggttg tggctgaaac ccagcatttg attcgacctg
    27061 agtttggtga cgattcacaa attgatatcc ggaaagggcg ccatgctgtc gttgaaaagg
    27121 ttatgggggc tcagacctat attccaaata cgattcagat ggcagaagat accagtattc
    27181 aactggttac agggccaaac atgagtggga agtctaccta tatgcgtcag ttagccatga
    27241 cggcggttat ggcccagctg ggttcctatg ttcctgctga aagcgcccat ttaccgattt
    27301 ttgatgcgat ttttacccgt atcggagcag cagatgactt ggtttcgggt cagtcaacct
    27361 ttatggtgga gatgatggag gccaataatg ccatttcgca tgcgaccaag aactctctca
    27421 ttctctttga tgaattggga cgtggaactg caacttatga cgggatggct cttgctcagt
    27481 ccatcatcga atatatccat gagcacatcg gagctaagac cctctttgcg acccactacc
    27541 atgagttgac tagtctggag tctagtttac aacacttggt caatgtccac gtggcaactt
    27601 tggagcagga tgggcaggtc accttccttc acaagattga accgggacca gctgataaat
    27661 cctacggtat ccatgttgcc aagattgctg gcttgccagc agacctttta gcaagggcgg
    27721 ataagatttt gactcagcta gagaatcaag gaacagagag tcctcctccc atgagacaaa
    27781 ctagtgctgt cactgaacag atttcactct ttgatagggc agaagagcat cctatcctag
    27841 cagaattagc taaactggat gtgtataata tgacacctat gcaggttatg aatgtcttag
    27901 tagagttaaa acagaaacta taaaaccaag actcactagt taatctagct gtatcaagga
    27961 gacttctttg acaattctcc acttttttgc tagaataaca tcacacaaac agaatgaaaa
    28021 ggagctgacg cattgtcgct cccttttgtc tattttttaa ggagaaagta tgctgattca
    28081 gaaaataaaa acctacaagt ggcaggcctt ggcttcgctc ctgatgacag gcttgatggt
    28141 tgctagttca cttctgcaac cgcgttatct gcaggaagta ttagacgccc tccttactgg
    28201 gaaatatgaa gctatttata gtatcggggc ttggttgatt ggtgtggccg tagtcggtct
    28261 agttgctggt ggactcaatg ttgtcctcgc agcctatatt gcccaaggag tttcatccga
    28321 ccttcgggag gatgccttcc gtaaaattca aaccttttct tatgctgata ttgaacaatt
    28381 taatgcggga aatctagtcg ttcgaatgac aaatgatatc aaccagattc agaacgttgt
    28441 catgatgacc ttccaaattc ttttcagact tcccctcttg ttcatcggtt cgtttatcct
    28501 agcggttcaa accttacctt ctctgtggtg ggtgattgtt ctcatggtag tcttgatttt
    28561 tggtttgact gctgtcatga tgggaatgat ggggcctcgt tttgccaagt ttcaaaccct
    28621 tcttgagcgc atcaatgcca ttgccaagga aaatttacgt ggcgttcgtg tggtcaagtc
    28681 ctttgtccaa gaaaaagagc aatttgctaa gtttacagag gtctcagacg agctttttgg
    28741 tcaaaacctt tacattggtt atgccttttc agtagtggaa ccctttatga tgttggttgg
    28801 ttacggggcg gtcttcctct ctatttggct ggtcgcggga atggttcagt cggatccgtc
    28861 tgttgttggt tccatcgctt cttttgttaa ttacctaagc cagattatct ttaccattgt
    28921 tatggttgga tttttgggaa attctgtcag ccgtgccatg atttccatgc gtcgtattcg
    28981 agaaattctt gacgcagagc cagctatgac cttcaaggat atcccagatg aagagttggt
    29041 tggaagcctt agctttgaaa atgtgacctt tacctatcca atggacaagg aaccgatgct
    29101 gaaagatgtg agctttacta ttgaacctgg tcaaatggtt ggtgtagttg gagcgactgg
    29161 tgcaggaaag tcaaccttgg ctcaattgat tccacgtctc tttgatccac agaacggggc
    29221 cattaaaatc ggtggcaagg atattcgaga agtgagtgaa ggaaccctgc gtaaaacagt
    29281 ttccatcgtt ctccaacgtg ccattctttt tagtggaacg attgcagata acttgagaca
    29341 ggggaaggga aatgctactc tatttgaaat ggagcgcgca gccaatattg cccaggctag
    29401 tgaattcatt catcgtatgg agaaaacctt tgaaagtcca gttgaagaac ggggaaccaa
    29461 tttctctggt ggacaaaaac aaaggatgtc gattgcgcgt gggattgtca gcaatccacg
    29521 tattctgatt tttgatgatt cgacctcagc cttggatgcc aaatcagagc gcttggtgca
    29581 agaagctttg aataaggact tgaaggggac gacaaccatt attattgctc aaaaaattag
    29641 ctcggttgtc catgcagaca agatcttggt tctaaatcaa ggacgattga ttggtcaagg
    29701 tacgcatgca gacttggttg ccaacaatgc cgtttaccgt gaaatctatg aaacacagaa
    29761 atgaaagaca aactataaga aaagtcaata gttttatcta aactatttct tatttcaatt
    29821 tgatgatttg gcgatgattt tagagcacgg caaaaggccc ttgaaaaagt ccattttttc
    29881 aaaggtaatc ctgtgttaat ttcagaaatt acatcacttt ttgttcgtca aatggcagct
    29941 ctttttttag gatataaaac agggttcgga taagtttttt tgcaaggtgg atgatggcta
    30001 cattgtaatg ttttccttgt tctaatttag tcttaagata ggccttaaaa gcaggcgaaa
    30061 agcgagggca tgctttggca gcttgtatga gtacctaccg cagatgaggg gaactccgtt
    30121 tgaccattct tcctgctaaa tcaatctgat ctgactgata aatagaagaa tccagtccag
    30181 cgaaagcttg taattgagca ggattatcaa aggcatgaat atttcgaatc tcagctaaaa
    30241 tgaccgcccc taaacgatcc ccaatcccag taaccgtcgt gatgaccgag ttgaactcag
    30301 ccatcaagtc attgacacat gtttccgcct tgtcaatgag ccccttgtaa tgtttgatgt
    30361 tttcattaca cgagataaaa cgtctatgcg ttatcaaact cattaccaat taaaacaaaa
    30421 agctgtggtt agatcctttc ggaaattgtc aagcgattgg aggaaatgaa ctaatccaca
    30481 gcggcttatt ccaagtatac cacttgggct ttggcagtag ctaactgcgc taaatataat
    30541 ataaggagga gtaaaatgaa gacagttcaa tttttttggc attattttaa ggtctacaag
    30601 ttctcatttg tagttgtcat cctgatgatt gttctggcga cttttgccca agccctcttt
    30661 ccagtctttt ctggacaagc ggtgacgcag ctagccaatt tagttcaagc tcatcaaaat
    30721 ggcaatccag aacttgtatg gcaaagccta tcaggaatca tggtcaatct tggcctgctg
    30781 gttttggttc tatttatctc tagtgtaata tacatgtgtc tcatgacgcg cgtgattgca
    30841 gaatcgacca acgagatgcg caaaggtctc tttggtaagc ttgctcagtt gacggtttct
    30901 ttctttgacc gtcgacaaga tggcgatatc ctgtctcatt ttaccagtga tttggataat
    30961 atcctccaag cctttaacga aagcttgatt caggtcatga gcaatattgt tttatacatt
    31021 ggtctgattc ttgtcatgtt ttcgagaaat gtgacgctgg ctctcatcac cattgccagc
    31081 accccattgg ctttccttat gctgattttc atcgtgaaaa tggcacgcaa atacaccaac
    31141 ctccagcaga aagaggtagg gaagctcaac gcctatatgg atgagagcat ttcaggccaa
    31201 aaagccgtga ttgtgcaagg aattcaagag gatatgatgg caggatttct tgaacaaaat
    31261 gagcgcgtgc gcaaggcaac ctttaaagga agaatgttct caggaattct tttccctatc
    31321 atgaatggga tgagcctgat taatacagcc atcgtcatct ttgctggttc ggctgtactt
    31381 ttgaatgata agtctattga aacaagtaca gccctaggtt tgattgttat gtttgcacaa
    31441 ttttcacagc agtactacca gcctattatc caagttgcag cgagttgggg aagccttcag
    31501 ttggccttta ctggagctga acgaattcag gaaatgtttg atgcagagga ggaaatccga
    31561 cctgaaaagg ctccaacctt cactaagttg caagaaagtg ttgaaatcag tcatatcgat
    31621 ttttcatact tgcctgataa acctattttg aaagatgtca gcatttctgc ccctaaaggc
    31681 cagatgacag cagttgttgg gccgacaggt tcaggaaaaa cgactattat gaacctcatc
    31741 aatcgctttt atgatgttga tgctggtggt atttattttg atggtaaaga cattcgtggc
    31801 tatgacttag atagtcttag aagcaaggtg ggaattgtat tgcaagattc ggtcttgttt
    31861 agcggaacga ttcgagacaa tatccgattt ggtgtgccag atgctagtca ggaaatggtt
    31921 gaggtagcag caaaagcaac ccacattcac gactatatcg aaagtttgcc tgataagtac
    31981 gatactctta ttgatgatga ccagagcatc ttttcaacag ggcagaagca attgatttca
    32041 atcgctcgaa ccctgatgac agatccagaa gttctcattc tcgatgaagc aacttcaaac
    32101 gtagatacgg tgacagaaag caagattcag catgccatgg aggtggttgt agcaggtaga
    32161 actagtttcg tcattgccca ccgcttgaaa accattctca atgcagatca gattattgtc
    32221 cttaaagatg gagaagtcat tgaacgtggt aaccaccatg aacttttgaa gctaggtggc
    32281 ttttattcag aactctatca caatcaattt gttttcgaat aagaaagaag ttgtcctatg
    32341 tgggcagctt tttcttgtcc ataaaaaatg tttatcacag ccttaaaaaa aacatattag
    32401 acgaaagtca ttttgagtga tatgatagga ctatcgttag cattcgaaag gagaggcatc
    32461 atggctagaa cggttgtagg agttgctgca aatctatgtc ccgtagacgc agaaggcaaa
    32521 atcattcatt catctgtatc ttgtagattc gcagagatca ttcgtcaagt cggtggtctc
    32581 cctttagtca ttcctgttgg tgatgagtca gttgtacgtg attatgtgga aatgattgac
    32641 aaactcattt tgacaggagg ccaaaatgtt catcctcagt tttatggaga gaagaagacc
    32701 gtcgagagcg atgattacaa tctggtccgt gacgaatttg aattggcact cttgaaggaa
    32761 gcgcttcgtc agaataaacc aattatggca atctgtcgcg gtgtccaact tgtcaatgtt
    32821 gcctttggtg gaaccctcaa tcaagaaatc gaaggtcact ggcaaggact acctttcggg
    32881 acatctcact ctattgagac agtagaagga agcgtggtgg ctaagctatt tgggaaagaa
    32941 agtcaggtca actcagtcca tcgtcaaagc attaaagatt tggcacctaa tttccgtgta
    33001 actgctattg attcaagaga ccaaactatt gaagcgattg agtctatcga cgagcaccgc
    33061 attatcggtt tacagtggca tccagagttt ctggttaatg aagaagatgg caatttagaa
    33121 ttatttgagt atttattgaa tgaactgtaa cgattggaac atctggtcgt tctttctttt
    33181 attttttcaa aatttttaga atgtggcatt ttacgcaaac gtttgaattc tgataaaaat
    33241 tggagaaatt cgacaaaaaa acttgaaaaa aacgaaggta agcgttatga tagaaaagaa
    33301 gaaatattgg aggaagaaca tgtcacatat taaatttgat tattcaaaag ttttagacaa
    33361 atttgttgca ccacatgaag tggaatacat gcaatcacaa gtaacagcag cagatgaatt
    33421 gatccgtaaa ggaactggtg ctggtagcga cttcttggga tggttggacc ttcctgaaaa
    33481 atacgaccgc gaagaatttg accgcatctt gaaagctgct gagcaaatca agtcagacag
    33541 cgatgttttg gttgtgatcg gtattggtgg atcttacctt ggtgcaaaag cagcaatcga
    33601 cttcttgaat caccattttg ctaacttgca aacaaaagaa gaacgcaaag ctccacaaat
    33661 cctttacgca ggaaactcaa tctcatctac ttaccttgct gacttggtag agtatgttgc
    33721 agacaaagac ttctcagtaa acgtaatttc taaatcaggt acaacaactg aaccagcgat
    33781 tgctttccgt gtctttaaag aactcttggt taagaaatac ggtcaagaag aagctaacaa
    33841 acgtatctat gcaacaactg accgccaaaa gggtgctgtt aaggttgaag cagacgctaa
    33901 cggttgggga acatttgttg ttccagatga tatcggtgga cgcttctcag tattgacagc
    33961 cgttggtttg cttccaatcg cagcatcagg agctgacata aaagctctta tggaaggtgc
    34021 gaatgcagct cgcaaagact acacttcaga caaaatctct gaaaacgaag cttaccaata
    34081 cgcagtcgtt cgtaacatcc tttatcgtaa aggctatgca actgagatct tggtaaacta
    34141 tgagccatca cttcaatact tctcagaatg gtggaaacaa ttggctggtg aatcagaagg
    34201 aaaagaccaa aaaggtatct acccaacttc agccaacttc tcaactgact tgcactcact
    34261 tggtcaattt atccaagaag gaactcgtat catgtttgaa acagttgtcc gtgttgacaa
    34321 acctcgtaaa aacgtgctta ttcctacttt ggaagaagac cttgacggac ttggttacct
    34381 tcaaggaaaa gacgttgact ttgtaaacaa aaaagcaact gacggtgttc ttcttgccca
    34441 cacagatggt gatgtaccaa acatgtatgt gactcttcca gagcaagacg ctttcactct
    34501 tggttacact atctacttct tcgaattggc aattgccctt tcaggttact tgaatgctat
    34561 caacccattt gaccaaccag gtgttgaagc ttataaacgt aacatgtttg cccttcttgg
    34621 aaaaccagga tttgaagaat tgagcaaaga acttaacgca cgtctataat agaagaaaag
    34681 agtggtttgc ccactctttt tactctcttt atccatagaa attggactca gccaagactt
    34741 gtgatataat atagaaagca aaaaggcaga cgcctagata ataggagaaa ctatgtcaaa
    34801 agatatccgc gtacgttacg caccaagtcc aacaggacta ctacacatcg gaaatgcccg
    34861 tacagcattg tttaactact tgtatgcgcg ccatcatggt ggaacatttc tcatccgtat
    34921 cgaagatact gaccgtaaac gccatgtcga ggatggtgaa cgttcacaac ttgaaaacct
    34981 tcgctggtta ggcatggatt gggatgaaag tccagaatca catgagaatt atcgccagtc
    35041 tgagcgtttg gacttgtatc aaaaatacat cgaccaacta ttagctgaag gaaaagccta
    35101 caaatcttac gttacagaag aagagttggc agctgaacgt gaacgtcaag aagtagctgg
    35161 cgaaacacca cgctacatca atgaatacct tggtatgagt gaagaagaaa aagcagctta
    35221 catcgcagaa cgtgaagcag cagggatcat cccaactgtt cgtttggctg tcaatgagtc
    35281 aggtatctac aagtggcatg atatggtcaa aggcgatatc gaatttgaag gtggcaatat
    35341 cggtggtgac tgggttatcc aaaagaaaga cggttaccca acttacaact ttgccgttgt
    35401 tatcgatgac cacgatatgc aaatctctca tgttatccgt ggagatgacc atattgctaa
    35461 tacaccaaaa cagcttatgg tctatgaagc tcttggttgg gaagctccag agttcggtca
    35521 catgaccttg attatcaact ctgagactgg taagaagtta tctaaacgtg atactaatac
    35581 acttcaattt atcgaagatt accgaaagaa aggttacctt ccagaagcag tctttaactt
    35641 tattgctctt cttggttgga acccaggtgg tgaagatgag attttctctc gtgaagaact
    35701 cattaaactt tttgatgaaa accgcctcag caagtcacca gcagcctttg atcagaaaaa
    35761 actagactgg atgagcaatg attatatcaa gaatgcagac cttgaaacca tctttgaaat
    35821 ggcaaaacca ttcttagagg aagcaggccg tttgactgac aaggctgaaa aattagttga
    35881 gctctataaa ccacaaatga aatcagtaga tgagattatc ccattgacag atcttttctt
    35941 ctcagatttc ccagaattga cagaagcaga gcgcgaagtc atgacgggtg aaacagttcc
    36001 aacagttctt gaagcattca aagcaaaact tgaagcgatg acagatgata aatttgtgac
    36061 agaaaatatc ttcccacaaa ttaaagcagt tcaaaaagaa acaggtatta aagggaaaaa
    36121 tcttttcatg cctattcgta tcgcagtttc aggcgaaatg catgggccag aattaccaga
    36181 tacaattttc ttgcttggac gtgaaaaatc aattcagcat atcgaaaaca tgctaaaaga
    36241 aatctctaaa taaagaggat acaataatgg acatctggga aaagatgtac gaagaagcac
    36301 agaaactata caatccacat gaagtatcag actttgttta tgccaatcat gttgtagctg
    36361 cagtagaagc agaagatgga caaatattta caggattctg tatggaggga acctgtggtg
    36421 ttttccatct ctgcgcagaa cgggcgaccc tcttcaatat gtaccaattt tcaggacaaa
    36481 ctaaggttaa gaaagtatta gcctttcgag acaaaccacc ttatggtgga agttcagcca
    36541 tgccttgcgg tgcttgtaga gaattccttt tagagcttaa tgctgaaaat aaagatgcag
    36601 aattcatgat ggactataat ataagaaaaa cagttaaagt cgcagaacta atcccttatt
    36661 ggtggggaga agaacgtgct tctaagttta atgagcg
//
LOCUS       DAVLYR010000006        36060 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLYR010000006 DAVLYR010000000
VERSION     DAVLYR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36060
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.006"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(79..3756)
                     /gene="rpoC"
                     /locus_tag="VYO63_000289"
     CDS             complement(79..3756)
                     /gene="rpoC"
                     /locus_tag="VYO63_000289"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1671273.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(3789..7400)
                     /gene="rpoB"
                     /locus_tag="VYO63_000290"
     CDS             complement(3789..7400)
                     /gene="rpoB"
                     /locus_tag="VYO63_000290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1671274.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            7816..7886
                     /locus_tag="VYO63_000291"
     tRNA            7816..7886
                     /locus_tag="VYO63_000291"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7848..7850,aa:Cys,seq:gca)
     gene            complement(8084..9415)
                     /locus_tag="VYO63_000292"
     CDS             complement(8084..9415)
                     /locus_tag="VYO63_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1671275.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(9644..10468)
                     /gene="endA"
                     /locus_tag="VYO63_000293"
     CDS             complement(9644..10468)
                     /gene="endA"
                     /locus_tag="VYO63_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1671276.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(10507..10695)
                     /locus_tag="VYO63_000294"
     CDS             complement(10507..10695)
                     /locus_tag="VYO63_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1671277.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(10688..11971)
                     /gene="murA"
                     /locus_tag="VYO63_000295"
     CDS             complement(10688..11971)
                     /gene="murA"
                     /locus_tag="VYO63_000295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1671278.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(11995..12225)
                     /locus_tag="VYO63_000296"
     CDS             complement(11995..12225)
                     /locus_tag="VYO63_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1671279.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(12283..13320)
                     /locus_tag="VYO63_000297"
     CDS             complement(12283..13320)
                     /locus_tag="VYO63_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1671280.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(13304..13792)
                     /gene="coaD"
                     /locus_tag="VYO63_000298"
     CDS             complement(13304..13792)
                     /gene="coaD"
                     /locus_tag="VYO63_000298"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1671281.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(13782..14321)
                     /gene="rsmD"
                     /locus_tag="VYO63_000299"
     CDS             complement(13782..14321)
                     /gene="rsmD"
                     /locus_tag="VYO63_000299"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1671282.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(14386..15378)
                     /locus_tag="VYO63_000300"
     CDS             complement(14386..15378)
                     /locus_tag="VYO63_000300"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1671283.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(15490..16173)
                     /locus_tag="VYO63_000301"
     CDS             complement(15490..16173)
                     /locus_tag="VYO63_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1671284.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16229..16969)
                     /locus_tag="VYO63_000302"
     CDS             complement(16229..16969)
                     /locus_tag="VYO63_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1671285.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSPALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17003..17281
                     /locus_tag="VYO63_000303"
     CDS             17003..17281
                     /locus_tag="VYO63_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1671286.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17359..18285
                     /gene="yidC"
                     /locus_tag="VYO63_000304"
     CDS             17359..18285
                     /gene="yidC"
                     /locus_tag="VYO63_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1671287.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVHEELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(18360..19154)
                     /gene="pflA"
                     /locus_tag="VYO63_000305"
     CDS             complement(18360..19154)
                     /gene="pflA"
                     /locus_tag="VYO63_000305"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1671288.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(19280..20530)
                     /locus_tag="VYO63_000306"
     CDS             complement(19280..20530)
                     /locus_tag="VYO63_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1671289.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(20597..21424)
                     /gene="purR"
                     /locus_tag="VYO63_000307"
     CDS             complement(20597..21424)
                     /gene="purR"
                     /locus_tag="VYO63_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1671290.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(21691..22632)
                     /locus_tag="VYO63_000308"
     CDS             complement(21691..22632)
                     /locus_tag="VYO63_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1671291.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(22622..23878)
                     /locus_tag="VYO63_000309"
     CDS             complement(22622..23878)
                     /locus_tag="VYO63_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1671292.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(23880..24542)
                     /locus_tag="VYO63_000310"
     CDS             complement(23880..24542)
                     /locus_tag="VYO63_000310"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1671293.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(24505..25161)
                     /locus_tag="VYO63_000311"
     CDS             complement(24505..25161)
                     /locus_tag="VYO63_000311"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1671294.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVANPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(25172..26050)
                     /gene="rsgA"
                     /locus_tag="VYO63_000312"
     CDS             complement(25172..26050)
                     /gene="rsgA"
                     /locus_tag="VYO63_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1671295.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFFNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETHKKVSKKIPK"
     gene            complement(26052..26924)
                     /gene="rsmA"
                     /locus_tag="VYO63_000313"
     CDS             complement(26052..26924)
                     /gene="rsmA"
                     /locus_tag="VYO63_000313"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1671296.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFACLADALKGQGL"
     gene            complement(27055..27363)
                     /locus_tag="VYO63_000314"
     CDS             complement(27055..27363)
                     /locus_tag="VYO63_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671297.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(27356..27997)
                     /locus_tag="VYO63_000315"
     CDS             complement(27356..27997)
                     /locus_tag="VYO63_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671298.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRMIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(27999..30029)
                     /locus_tag="VYO63_000316"
     CDS             complement(27999..30029)
                     /locus_tag="VYO63_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1671299.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREKVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSI
                     LGLGILWYQGALFMATVQLVIIALLLYGLILAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQYSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(30298..31197)
                     /locus_tag="VYO63_000317"
     CDS             complement(30298..31197)
                     /locus_tag="VYO63_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1671300.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(31269..31829)
                     /gene="rnmV"
                     /locus_tag="VYO63_000318"
     CDS             complement(31269..31829)
                     /gene="rnmV"
                     /locus_tag="VYO63_000318"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1671301.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSCKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(31829..32602)
                     /locus_tag="VYO63_000319"
     CDS             complement(31829..32602)
                     /locus_tag="VYO63_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1671302.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(32907..33800)
                     /locus_tag="VYO63_000320"
     CDS             complement(32907..33800)
                     /locus_tag="VYO63_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1671303.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(34084..34218)
                     /gene="rpmH"
                     /locus_tag="VYO63_000321"
     CDS             complement(34084..34218)
                     /gene="rpmH"
                     /locus_tag="VYO63_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1671304.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(34363..35577)
                     /locus_tag="VYO63_000322"
     CDS             complement(34363..35577)
                     /locus_tag="VYO63_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1671305.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(35733..36027)
                     /locus_tag="VYO63_000323"
                     /pseudo
     CDS             complement(35733..36027)
                     /locus_tag="VYO63_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10125 a         8454 c         6970 g        10511 t
ORIGIN      
        1 tttaccacat ttcgtggaaa aatggagaaa gttttcagaa gagagaatga gagaaccctc
       61 gggttctctc attctctctt attctactgt ttcttccaca gtttcaacgg cagtatccac
      121 aactacttct gttgtttctt catttccttc ttcctctact ggaggattaa ggtattcttc
      181 ttcgttgaca gcatgtggtt caaggttacg gtaacgggcc ataccagtac cagctgggat
      241 gatcttaccg atgataacat tttctttaag tccaaggaga tggtctttct taccacggat
      301 agctgcgtca gtaaggacac gagttgtttc ctggaaggaa gccgctgaca agaaactgtt
      361 tgtttcaagt gaggctttgg taattcccat aaggactggg cgacctgtcg ctggaactcc
      421 acctgcgata aggacatctt tgttggcatc tgtaaagtca ttgatatcca tgagggtacc
      481 catgagaaga tctgtatcac ctggatccat gacacggact ttacggatca tttgacgaac
      541 cattacctcg atgtgtttgt caccgatttc taccccttgg ctacggtaaa ctttttgtac
      601 ttcaccgaga aggtacgttt caactgacaa gacatcacga actgcaagga gacgttttgg
      661 ttggatagaa ccttctgtca gagcagcacc acgcgctact tggcccccaa cttcgacacg
      721 catacgagct gtaaatggaa cgacatattc accttcgcca gtttcaccct taacaaagac
      781 tttcttggta cgagttgatg catcttcttc gatagcagta acttgtcctt taacctctgt
      841 aataaccgct tcccctttag gattgcgggc ttcaaagatt tcttggacac gaggaagacc
      901 ctgagtgata tcggtatttg aggcaacccc acctgtgtgg aaggtacgca ttgtaagctg
      961 tgtaccaggt tccccgatag attgggcagc gattgtacca actgcttcac caacttcaac
     1021 cgcatcacca gtcgccaagt tgataccgta acagtgacgg cagacaccgt gacgagtgtt
     1081 acatgtaaat acagaacgga tagtcacttc ttccacacca gcattgacaa tttcacgcgc
     1141 cttgtcttct gtaatcaatt catttggacc aataatcact gcaccagttt ctggatgttt
     1201 aacagttttc ttagtgtaac gaccgttgag acgctcttcg agagactcga tcatctcttt
     1261 tccttctgcg atagaacgga tcaagagacc acggtcagtt ccacagtcgt cctcacggat
     1321 gataacgtct tgggcaacgt cgaccaaacg acgagtcaag taacctgagt cggctgtctt
     1381 aagggccgta tcggtcatac ctttacgagc accgtgagtt gagaagaaca tttccaatac
     1441 cgacaaacct tcgcggaagt ttgaaaggat tggcaattcc atgatacgtc cattcggagc
     1501 agccatcaga ccacgcatac cggcaagctg tgagaagttt gagatgttac cacgggctcc
     1561 agagtccatc atcataacga ttgggttctt aggatcttga ttagcaatca agcgtttctc
     1621 aagtttttca cgggcagcac gccattcagc tgtaacagca ttgtaacgct cgtcgtctgt
     1681 gatcatacca cgacggaatt gtttggtgat ttgttcgaca cgtttgtgtg attcttcaat
     1741 gatttcagcc ttgtcatcaa cgactgggat atcggcaata cccactgtca atcctgcaag
     1801 agttgagtgg tggtaaccga ggttcttcat gcggtcaagt agggcagaag tttctgtcgt
     1861 acggaaacgt ttgaagattt cagcgatgat atttccgagg tttttcttct tgaatggagg
     1921 gttgagctca agattgctga tagcttcctt gatagctcca ccaagtggca agaagtattt
     1981 agctggaaca ccttctgtca agttggcatt gtttggttct tgcaagtatg gtagcccctc
     2041 tggcatgata tcgttgaaga gaattttacc aactgttgta agcaagacct tatgtctttg
     2101 ctcttctgtc caaggcttgt tgaggctgtc tgttgcgata ccaacacgtg agtggaggtg
     2161 aacataacca ttgcggtaag ccataaccgc ttcgtcacgg tctttgaaga ccattccttc
     2221 accttcgcga ccagcttctt ccatggtcaa gtagtagtta cccaaaacca tgtcctgaga
     2281 tggagtaact accggtttcc catctttcgg gttcaagatg tgctcagcag ctagcatgag
     2341 gatacgagct tctgcttgtg cttcttctga aagtggtacg tggatggcca tttggtcccc
     2401 gtcaaagtca gcattgtagg cttcacagac aagtgggtgc aagcgaagag ccttaccatc
     2461 aattaagact ggctcgaagg cttggatacc caaacggtga agggtcggtg cgcggttcaa
     2521 aagcactggg tgttctttaa tcacttcttc aaggatatcc cagatacgct catctccgcg
     2581 ttccaccaag cgtttagctg ctttgacgtt ttgcacgata tcacgggcaa cgatttcacg
     2641 catgacaaat ggtttaaaga gttcaatcgc catttcacgt ggcacaccac attggtacat
     2701 cttaagagtt ggaccaacgg cgataacgga acgtcctgag aagtcaacac gtttaccgag
     2761 caagttttga cggaagcgtc cttgtttacc tttaagcatg tggctcaatg atttcaatgg
     2821 acggctacct ggtcctgtga ttggacgacc acgacgacca ttgtcaatca aagcatcaac
     2881 tgcttcttga agcatacgct tctcattttg aacgatgata cctggtgcat ttaactcaag
     2941 caaacgagcc aaacggttgt tacggttgat aacacggcgg taaaggtcat tcaagtcaga
     3001 tgaggcaaaa cggccaccat ccaactgcaa cattggacga agatctggtg ggataaccgg
     3061 aaggatgtta agaatcatcc attcaggttt gtttccagac ttgtaaaagg catccaaaac
     3121 atccaaacga cggatggctt tgacacgttt ttgtccagta gctgttttca attcttcttt
     3181 gagttcagca atttcttttt caagatctac ttgcttcaaa aggtcttgga tggcttccgc
     3241 acccatcttg gcaacaaatg aaccataacc atattcacgc aagcgctctc ggtattcgcg
     3301 ctctgtcatg atagacttgt gctcaagtgg tgtatcctta ggatcaatca ccacataagc
     3361 cgcaaagtag ataacttcct cgagggcacg agggctcata tcaagggtca agcccatacg
     3421 gcttggaatc cccttgaagt accagatgtg agatacagga gctttcaatt cgatatgtcc
     3481 catacgctca cgacgaactt tcgtacgcgt tacttcaacc ccacagcggt cacaaacaat
     3541 tcctctgtaa cgaatgcgtt tgtacttacc acaagcacat tcccagtctt ttgtaggacc
     3601 aaagatcact tcatcaaaga gtccttcacg ttctggtttc aaggtacgat aattgattgt
     3661 ttcaggtttt ttgacttctc cataagacca tgaacggact ttacttggag aagctagggt
     3721 gatttgcata cttttaaaac gatttacatc aaccactatt tcttcccttt ctattctaag
     3781 tgaactgctt attcttgttc agcagcttct tctgttgctt ccgcttttgt tgctttctca
     3841 gcttcttcag cttcaaaggc tgctttagcc tcttgggctg ctttttcgcg ggctttttca
     3901 aggtcatcta cgtggatgac atcttcgtcc attccttcat ccaagtcgcg aagttccact
     3961 tcttggtcat cttcgtctag gacacgcatg tcaagaccaa gagattgcaa ttctttgaca
     4021 agaactcgga aggattctgg aacacctggt tttggaattg gtttgccttt tgtaatagct
     4081 tcataggctt tcaaacgtcc gttgatatcg tccgacttgt aagtcaagat ttcttgaagg
     4141 acatttgacg caccgtaggc ttcaagagcc caaacctcca tctcaccgaa acgttgtcca
     4201 ccaaactgag ctttacctcc gagtggttgt tgggtaacag ttgagtaagg tccgactgaa
     4261 cgcgcgtgca atttatcgtc aaccatgtgg tggagtttga tcatgtacat gactccaaca
     4321 gaaacacggt tatcaaatgg ttcacctgta cgtccatcgt aaaggattgt cttggcatcg
     4381 ctatccatac ctgcttcttt aacagttgac caaagatctt cagaacttgc tccatcaaag
     4441 actggtgtcg caatgtgaat accaagagta cgagctgcca taccaaggtg aagctccata
     4501 acctgaccga tattcatacg tgatggcacc ccaagtgggt tcaacatgat gtcgactgga
     4561 gttccgtctg gaaggtaagg catgtcttct acaggaacga tacgagagac aacccctttg
     4621 tttccatgac gtccggccat tttatctccg accttaatct tacgtttttg agcgatgtaa
     4681 acacgaacca acatgttaac acctgattgc aactcatctc catttacacg tgtaaagatc
     4741 ttaacatcac gaacgacacc atcggcaccg tgtggtacac gaagagaagt atcacgcact
     4801 tcacgagact tgtctccaaa gatagcgtgc aagagacgtt cttcagctga aagatctttc
     4861 tcacccttag gtgttacttt acctacaaga atatcacctt ctttaacctc agcaccaata
     4921 cggataatcc ccatttcgtc aaggtctttg agggcatctt caccaacgtt tggaatttcg
     4981 cgagtgattt cttcaggccc aagctttgta tcgcgcgttt ctgattcgta ttcttcaagg
     5041 tgaacagatg tgtagacatc gtccttcacc aagcgttcgc tcatgataac ggcatcctcg
     5101 aagttgtaac cttcccaagt catgtaggca acgattgggt tttgtccaag cgccatttct
     5161 ccattttcca tagaaggtcc gtcagcgatg aaatcgcctt tttcaacgac atcaccaact
     5221 tttacgagag tgcgttggtt gtaagcagta cctgagtttg aacgacggaa tttttggatg
     5281 tggtaaacat ccaatgaacc atcttcacga cgaacttcta ccttgtcagc atctgcgtaa
     5341 gtaactttac catcatactg agcaatcaca gccgcaccag aatcgtgggc tgcttggtat
     5401 tccataccag taccaacgta aggtgcctga ggattaatca atggcacagc ctgacgttgc
     5461 atattggctc ccatgagggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacctg ttttggtgaa acgtccatgt agtcaacaat attagctgga
     5581 tactcttggt tgaccccttg gtgacgtccc atgacaatct tctcagcaaa ggttccatct
     5641 tcattcagac gagagttagc ctgagctaca gtatattcat cttcttcatc agctgtcaac
     5701 caaacaattt cgttcgtgac aacacctgtt tcacggtcaa ccttacggta tggtgtttga
     5761 acaaaaccat atttgttcaa gtgtccgtaa gatgacaagt tattgatcaa accgatgtta
     5821 ggtccttcag gtgtttcgat tggacacata cgaccatagt gagtgtagtg cacgtcacgc
     5881 acttcatatc cggcacggtc acgagtcaaa ccaccaggtc ctaaggctga caaacggcgt
     5941 ttgtgagaca actcagaaag cgggttgtgt tggtccatga actgtgacaa ctgtgatgaa
     6001 ccaaagaatt ctttaactgc agctgttaca ggacggatat tgataatttg ttgtggtgtc
     6061 aagacttcat tgtcctgaac agacatacgt tcacggacat tacgttccat acgagaaagt
     6121 cccaaacgta cttggttggc aagcaattca ccaaccgcac ggatacgacg atttccaagg
     6181 tggtcgatat catctacacg gccaagtcct tcagccaagt tgaggaagta gctcatctca
     6241 gcaaggatat ctgcaggagt caccgtacga accttgtcat ctgggttagc attaccaatg
     6301 atcgttacga cgcgatctgg atcagttgga gcaacaacct tgaatttttg aagaacaaca
     6361 ggctcagtca caacggctgc atcgtttggg atgtagacaa tcttgttcaa gtcgccatcc
     6421 aaatggcttt caatgctttc aatcacgcta cgagtcataa tcgtaccagc ttctaccaag
     6481 atttctccag tttcagggtc taccaatggc tctgcaatgg tttggttgag caaacgtgtt
     6541 ttaacattga gttttttatt gattttgtaa cgaccaactg ctgccaagtc atagcgacgt
     6601 gggtcaaaga agcgagccac aagcaagcta cgtgagcttt cagctgtctt aggctcacct
     6661 ggacgaaggc gttcgtaaat ttctttcaag gcttcgtctg tacgagagtc cattggattc
     6721 ttgtggatat ctttttcaac agtgttgcga accaattcgc tgtcaccaaa gatatcaaag
     6781 atttcatcat cacctgagaa accaagagca cgaaccaagg ttgtaaatgg aatcttacga
     6841 gtacggtcga tacgagtgta ggtgatatct tttgagtcgc tttcaagttc caaccaagct
     6901 ccacggttag ggataacagt tgaaccatag cccaccttac catttttgtc tactttgtcg
     6961 ttaaagtaaa cacctggtga gcggaccaac tgagaaacga taatacgttc accaccattg
     7021 atgatgaaag tacccatttc tgtcatgatt gggaaatcac caaagaaaac ttcttgggtc
     7081 ttgatttcgc ctgtttcttt attgatcaaa cggaaggtta caaaaattgg tgctgagtag
     7141 ctagcatcgt ggatacgagc ttcttctagc gtgtattttg gttccttgat ttcatatcca
     7201 acaaattcca actccattgt gtctgtgaag tttgaaattg gcaatacatc ttcaaacact
     7261 tccttaagac cgtggtctag gaaagctttg aatgagtcag tttgaatttc aatcaaattt
     7321 ggtaagtcaa gaacttcttt gattcttgaa aaactacgac gggtacgatg tttcccgtat
     7381 tgaacgtcat gtcctgccaa gatgattctc ctttgtaaat aagttccaag ccttgtcaat
     7441 caggcttttc taatcgtcat atggttgtaa accccttatc accgtgtcct cttgacgaat
     7501 tttcagaatc tttaagcctc tgttacaaat gctcataatc ttgaaaaaaa gcacaaaaag
     7561 agcagctaaa tctgactttt tcagaagatt taactgctgt gagccttgtc tggacaatat
     7621 ttcagacaaa acctacgaca aatgattacc catattatac cctatttagc tagatttttc
     7681 aaggggtttc agtaggtttt tggtaaattt tttcccatag aaaacttggc atcacattcg
     7741 aatcacgcta tggtacaaaa aactgaaaaa actattgact gaaaatcatt ttcaaggtat
     7801 aataataaac gttaaggcgg tatagccaag tggtaaggca cggctctgca aaagcttgat
     7861 cgtcggttca aatccgtcta ccgccttcta taacttgatt tatcaggttt caaatgaaca
     7921 gaaagcccaa tttgaagggc ttttcttatt ttccctcgaa taaatacgta taactttaaa
     7981 aacttttgga gcgagtttgt ggcagagttc tttccatggc ataattccct tttgaaatca
     8041 attgaaaaaa gcttcgtcac gcctggtgac aaaagcctct agtttattct gtttcttctt
     8101 ctatttcgac ttctgtgatt tggactttta ccttggtaac acgtccattc ttcactttat
     8161 cattggttaa gataaactgt ttgttttggc tgaccaattc atagctgagt ttctcagtcg
     8221 ttggaatagt tcccactcct gtcaaataat aaccagcgat ggtatcaacg tcatcacttt
     8281 ccagttcaac atcaaagtaa ttattgaaat cattgagggt catagttcct tgaacaatgt
     8341 aggtatcctc gccaatctga tgaacatcta ttgctgcttt gtccgtttca tcatcaattt
     8401 caccaacaat ttcctctaag aggtcttcca aagtcaccaa accagccata ccaccgtatt
     8461 catccagcaa gattgccatt tgtctttggg tatttcgcag ttcttttagc aagtcatcca
     8521 caaaaatagt ttcaggtaca aaaagtggat cttgtaaaat tctcttccaa acaatattgt
     8581 caaaaccgtc cacaaagcct gccttaagga gactcttggt gtgaatgatt ccaattacat
     8641 tgtccttatc cccatcataa accgggatac gagaataatt ttgttttaaa atactttgga
     8701 taatggcttg actatcatcc tgaatatcca ccataaaggc atccgttcga ggcaccataa
     8761 cctctcgtgc catcagttca tcgagcgaaa agacaccttg tagcatctca atctcatcag
     8821 catccaatgt ttcttcacta tttgtcagca tataggcaat ttcatcacgg gtcatctttt
     8881 catccgcatc atcgaatgac ataggagtca aatggctcaa gaaattggtc gaagcagcta
     8941 aaagccaaac aaaaggactg actagttttc cgatcccaat gataatcggc gctgtacgaa
     9001 ttgccaaggc atcctttaga ttaagagcga ttctcttagg atataattcc ccaaaaacga
     9061 tggaaatata ggtcaaaaat gccaaggata gaaaagttgc cacggcttgt gctgtttcgc
     9121 cattcccaag ccaagaggca atcacacgtc ctagagtatt agttaaactc gcccctgata
     9181 agattgtaat cagggtgatt cctacctgaa tggttgataa aaagtggtta ggattttcta
     9241 gtaccttcag caggcggatg tagcgtctgt ctccttcttc cgccttttgt tcaactcggg
     9301 cacgattaag agaaaccatg gccatttctg tggctgagaa aaaggcattt aataccgtca
     9361 agataaacaa taaaacaaat tgtagcaaca aattctgact acttgggtct tccatagttc
     9421 ttctcctaaa atagtatctt atcctttatt atatcacaaa atttaatcca atacatatta
     9481 ttttttctta ccgtaacttc tcaaagtaac ttccatctct ctcccaaaac tggaagttag
     9541 ttttagaagt tgctattttt tcttagtacc tgttctagcc ctgcttaaac tagtaaaaag
     9601 aactcctaca tccataagtg acataggagt ttaggcttat cttttactga gttacagtta
     9661 cttctccagt tcggtaatcc agttgaagtc ccttttgaac attgggaact agaacattga
     9721 attccaattc tccatccgaa gacttggctt caatctgtga agctgaggga actaaatcct
     9781 cgtttgaagc gtagtaaagg gttacacggt aacggacacg cttgttttgg tccaaggctt
     9841 tacgcacctt gctttcatag tagttttgac cagtcgaata ctcggcttgt gcctgatttg
     9901 cccaggctgt ctgaacagca atgtttttag gattgcttgt tgaggcatca aaaccatcca
     9961 aaccaccgat taaggcatag cctaacaaat gacctctatc gactgcatgg gtataagagc
    10021 cctttagatt cttgacctga tgccaacctg gaggagtcca agaagttgaa ccattcccag
    10081 tttctttacg attcttgtac tgacgagtgg ccttagacaa gagggcatta gctacggttg
    10141 gaacagtttc cttgcccact gtctttgttt tattgtcagc gtagggctta cttgaaacct
    10201 tggcatctag atttgtttta ttaccattga cgataaaagc acctgagcca ttccactcca
    10261 gactcccctt tatttgactc ttgactgcgt ctgttaagac actctctgcc aatgcttgac
    10321 taggagcttc agacgcttgt tttttctgac taagattggt tttgggacta ttaggtgccg
    10381 acggcatctg cttgatataa tagctccctg tagacaaaag caataacact agcagtccga
    10441 ttagtgtctg tcttgttttt ttgttcatat ttctccttat ctttagaaaa aggctggttc
    10501 tccagcctaa tttcctgtaa atttatgaat caattcctgc cattttgctg gagacaggat
    10561 agcccatgga tcttgaccct tgcccaagat tccataacct accattaaac cgattcctag
    10621 agccagagta cctaaaatca gtactatgat gactaaaagt aaacgcttga ctacgtagct
    10681 tgatttctta ttcatcttca tcacttgcct caatccgctg aatcttagca cctagctgcg
    10741 ccaacttctc atggaaaccg tagtaacctc tatccaagtg aaccaattta ccgaccacag
    10801 tttctccctg tgctaccaaa cctgtcaaaa tcaaggctgc actggcacga aggtcagttg
    10861 aaagaacttc tgctccctgc aaaggctgtc caccaacaat acgagctgta tcacggataa
    10921 tctcagaatg caagcccatg cggcgcatct cttctaggtg ttggaaacga ttttcgaaaa
    10981 ctgtctccac catggttgat tcgccttttg caactgtcat caaggctgta aattgagcct
    11041 gcatatctgt tggaaatcct gggtggggca aggttttcac atgaacagct tttagatttt
    11101 ctagttgaga acgaacacga attccttcgt cttcttcaat tacttcaaca cccatttcaa
    11161 gtaacttggc aatcaaggga cggttgtgct cccagacagc gtctcgaatc aagacatcac
    11221 caccagtcat ggcagcagct accataaagg ttcctgcttc gatacggtct tggactacat
    11281 tgtgagtcgt accatgaagt ttctcaacac cagtaatggt tatagtctct gtaccagcac
    11341 ctttgacctt ggctcccatt tcattaagga gaatggctaa gtcaacaatc tcaggctcac
    11401 gcgcagcatt ctcaatcact gtcaccccat cagccagagt cgctgccatc atcaagttct
    11461 gcgttgcacc aacacttgga aagtccatat agatatgagc gccatgcaag cgttctgcct
    11521 tggcttcgat gtaaccagct gtctgactaa tcttaacccc catagcttcc agacctttca
    11581 aatgaagatc aataggacgg ctaccaatcg tacaaccacc tggcatggat accttggcat
    11641 gacccacacg ggcaaggatt ggccctaata caacgatgga ggcgcgcatc ttgctgacat
    11701 acttgtaagg ggcttcctca gtgatgtcgc cagtagcatc caccttgaca agatgagctt
    11761 cctcatcaaa gtcaaccttg gcattcaaac caccaactac ctgattcata ataaagacat
    11821 ccgacaaaat cggaacattc tgcaagacgg tctttccttc acttgctaga atagtcgctg
    11881 ccaacaaggg taagactgca ttttttgctc cctcgatcgt cacgcttcct accagacgat
    11941 tatcgccacc ttgaaccaca attttatcca tactcgtttc ctttactctt gatttcataa
    12001 taatcctcta agcgcttctt gccacaaatg atacaggcta ataaagaaag aactcaggat
    12061 gtagcctatg acaatgctaa aaaaggctac taataaacga acttttcttg tattatcatc
    12121 tgtcactttt aaaaccttct cccatctaac aagatccttt aaaaggtaaa aactcacata
    12181 aataaagaac atatgactac ttagagtgaa taataattga accatttgac tattatacca
    12241 tcttctctgt ttgtgcgcta gtttggaaac ttcactcaaa aattagggat tgtttttcaa
    12301 gtaatcaatc gcatcttgta gggttttaac gggcacgatt ttcatatccg tcttgattgt
    12361 tttagccgct tctagggctg tttggtagtt gtttttcgcg tccggatgcg ccttttgttc
    12421 ttcttcgcta acagggttat caggagcaaa gaaaatagca gcacctgccc tagccgaagc
    12481 tacaaccttc ttatcaatgc ctccaatgtc tcccacatta ccatcgcggt caatggtacc
    12541 tgtaccggca acaatacgac cattacgaag atctgggtga gctatttgag tatagatagc
    12601 tagactaaac atgagaccag cacttgggcc gccaatacca gctgttgaaa agctaattgg
    12661 gacattgctg attacctctg tacgatcaat caagccgatt ccaattccat ttttgccatt
    12721 ttccaaggtg ataatctttc cttctgcaga cttggtttgc ccatcctctt cataggtgac
    12781 cttgacggaa tcccctaatt tttgagaact gacgtaatca atcaagtctt tggaactatc
    12841 aaaggtctga tcattgactg ctgtgactgt atcagagata ttgagaatcc ctttaaaggt
    12901 tgaattatcc gtcacattca aaacataaac tccaaagtac ttgagttcga tatccttacc
    12961 agctgttttt agtccttgat acttggccat attttgcgat gtttgcatgt agaattgatt
    13021 gattcgcata aattcaacat cggaagaacc acctgtagtc tcctgagcac tacgaatatc
    13081 tgtaaaaggt gtcaaccaag cataaatcat atgagctaaa gtggcatgtt gaacaccaac
    13141 cgtaacgaat tgataggcac cagcttcctt atcttctgtg tcatttactt taaggacttg
    13201 gcgaatatct tccgaaccac ctggaacctc tatataataa ggcaagggca ctacaaatgc
    13261 caagaaagtc acaatcaagg ccgcaatgac gtataagggc catctaatct tttttttcat
    13321 ttcttatttc ctccaaaata ctctcgggaa catggcaggc aatatcctga ccaaacttca
    13381 aaagctctcg aacacctgat gaactgatat agagatgttc aggccggcta tgtaaataaa
    13441 tagtctctat atcagaagac agctgatgat tgtagtaatc aaaactagct tcatattgca
    13501 aatccgccgc attcctcaac ccccgtacta ggaaagtagc ccccagtctt tttgcaacat
    13561 cgaccaccaa ttcatcatga gaagccacga cttcaacatt ttccaaatgt cccaaagcct
    13621 tttctagccc ccgtttacga ttttcaagag ggagaaatcc ttgtttgtgg ggattaaaaa
    13681 aaatacccac ataaagctta tcaaaaagtc tgctcgcccg ttcaatgata tccagatgcc
    13741 catttgtcat cggatcaaat gagcctgtga ataagccaat cttatctgac atagactgtc
    13801 accttactaa ttccataaat cttttccttc cagataccca gacaggcaat ctcttctgga
    13861 agttcaacgg ccttatccgt ctcgcacaca accataacat cttcagaaaa aagctctctc
    13921 ttagccattt tttcaatatc tgctacgatt tgttccttgg catagggagg gtctaagaaa
    13981 acgaggtcaa attccccaga tacctgttcc aatgccctct ctgcatccat cttgaggagt
    14041 tgaaattttc caacttcctt ggtcatctgg atattttcag ccacgatggt ctgagcctta
    14101 cggtctcgct ccaccaaaac agcactggac atgccacgcg atactgcttc gatagataaa
    14161 ccaccactac ctgcataaag gtccaagact cgtccccctt caaagtaggg accaatcatg
    14221 ttaaaaatgg ctcccctaac cttatccgaa gtaggtcttg ttgtcttgcc ttctagtgtc
    14281 ttgaggggac gtcccccata gattcctgat acgattttca tactgtttat tataccaaat
    14341 tatagacaaa aagagaaaga aaaccgaacc ttgcggttcg attctctaca aaatattttc
    14401 gtaagtatcg cgtacttctt gaggccaaac acttgtttgc acttctccga tgtgtctctt
    14461 gcgaagtagg aacatggcca tacgagattg tccaattcct ccaccgattg tcaatgggaa
    14521 taggccattc aacaaagact tgtgccattc caattccaag cggtcttcat cacctgtaat
    14581 ttccacctga cgtctaagag tttcttcatc tacacgaatt cccatagaag acaactcaaa
    14641 ggctccacct aaagactcat tccagacaag aatatcacca tttagaccct tgtagccatt
    14701 ctcagactcg cttgtccagt catcatagtc tggtgcacgt ccatcgtgcg gtttaccatc
    14761 tggcaactcg ccaccgatac caatcaaaaa gacggctcca aattctttac aaatcgcatt
    14821 ttcacgttct ttcgatgtca agtctggata gcgttctacc aattcttctg tatggataaa
    14881 ggtgatttgt tttggcaaga tagactcgat gtcatagcga gcttcaacag ctagctcagt
    14941 caggcgaata gccttataaa tcttctcaac tgtttctttt agataaacga tgttacgctt
    15001 accatttggg ataaccttct cccagtccca ctggtcaaca taaacagagt gggttgcatc
    15061 caaggaatcc tcatctggac gaagggcttt catgtggaca aagagaccct ctccttcacc
    15121 aaagccaaaa cgagccaagg tgtggcgttt ccatttagca agtgagtgca ccacttcata
    15181 agtagcatca gggatttgga gaaccttgac cgatactgga ttttccacac cagacaggtt
    15241 gtcctgcatt ccgtcaccga ccttactcaa gataggacct tgaacttcga caacttctag
    15301 cttatctttc aaatactggg taaaagtgtt tttgacaaag gaaatttctt cttgttgatg
    15361 gataaaactt ttcttcatag acaactcctc aaaaatttaa taaacgtatt atacacctat
    15421 ttccaagaaa agaaaagaga aaatttaaaa aaaatcagtg tcactcaaaa cactgatttc
    15481 atagaccttt tagtcattgc gaccaaagat acgtagaata cttaggaaaa gattgataaa
    15541 atcaagatag atgctgagag ccattgacac cacccagcct gtcgctacac gaccttgtga
    15601 ttgttcatag gcgaggcgaa tcttttggtt atcccaagca atcaaccctg aaaaaaccaa
    15661 aaccatggct acactaatca tataatcaaa gaagccacta gccaagaaaa tattgactac
    15721 catagcaatc agtaaaccaa tgagagctgc catcattgct cgaccaatcc cactcaagtc
    15781 tttcttggta aagataccaa ctgccgccat gacaaagaag agaagggcgc tcgatacaaa
    15841 ggcagataaa actgtacccg gagtatagaa agccaccaca aaactaaggg taaagccgtt
    15901 taaaacggag taaagtaaaa atactggaag agcagctgga ctatttcttg aagccatgct
    15961 actggcaacg aaaaccaaag ccaactccgc aaaagtagca atggtcaacc agagacgccc
    16021 ctgcatcaac aagtaaacca actgagactg aaagaccgtc aacataaggc ctgataccaa
    16081 agcagatagt ccgattccca gaccaacaaa ggcataaacc ttagcgtaaa attgattgag
    16141 acctgcacgg tcatgtataa tagtgtgatt catccttttc tccttttctg tgaacatctt
    16201 tccttgatta taacaaaaaa agttaaaatt agcttaaatg gaagattaaa ataccggctg
    16261 caactgcaac attcaaactc tcggcctgcc ccttcatgct aatatggacc aactggtctg
    16321 cactttcagc cataagggga ctaattcctt gaccctcatt tcccataact agtacaaaat
    16381 tttctataga aggcagttct ctgtaatcaa cagaatcttt agatagggtt gttgctagca
    16441 ctgggatagc taccttttta gtttcgtcaa gaagcgctgg actagtcatc cggtaaatgg
    16501 gcagatggaa atgactacct tgcatggaac gtagggtttt gagactgtag atgtctgccg
    16561 acttatctga aacaatcact cctgtaaaac ctgctgcatc cgcagttcga atgatagttc
    16621 ctacattacc gggatcttgc acatcttcca aaaacaagaa cttgccctga ctcaaatcag
    16681 cttgtcctac ttcttctttt tgaaccaccg ccacaattcc ctgtggagtc tgagaatccg
    16741 ccaaatctag caaaatatcc tctgaaatcc agacagtttg cggaaaagca gctaactgat
    16801 ctcgataatt ttctagggca aagattttct caatcgtcac tccagcttgg acagcttctt
    16861 caaacaagtg ccagccttca atcaaatagg cagacttgcg gtattttttt tggtgtaatt
    16921 tcttggcatt ttttaccaca gaattggctt ttgaggttat aatagtcata gaaatattat
    16981 aacacaatca aaggggtttg gtatgcaaaa ggttagaatg attgcccaag gtagggtaca
    17041 gggagtcggc tttcgttggg gtgtttacag cttggcactt gaaattggtg gcatcacagg
    17101 tcgagtatgg aataacgacg atggcacagt ggaaatctta gcccaagcag actcatctgc
    17161 tatcatggca aaatttatcc aagaaatccg aaaaggaccg acaccttttt caaaagtaag
    17221 ctacttagat gtcaaactaa gcaactttcc tccctactct gactttaaaa tcgcaaatta
    17281 ggtctccaga actattgtat attttgtaaa aaaacagtag aatagaaagg tataattttt
    17341 aaagaaggaa taaaaacagt gaaatctatt aaacgttttg cactctcagc tatgggagcg
    17401 gctatgttgc ttgtcttgac tggttgtgtc aatgtcgata aaaccacagg tcagccaaca
    17461 ggatttattt ggaataccat cggagcccct atggctgaag ctatcaagta cttcgctact
    17521 gataaaggtc taggctttgg tgtcgctatc atcatcgtaa ccattatcgt gcgcttgatt
    17581 atcttgccac ttggtatcta ccaatcatgg aaggcaacgc ttcactctga aaagatgaac
    17641 gccctcaagc acgtccttga gccacaccaa acgcgtctca aagaagcgac tactcaagaa
    17701 gaaaaactcg aagcccaaca agctctcttt gctgctcaaa aagagcacgg tatcagcatg
    17761 tttggcggtg taggatgttt ccctatcctc cttcaaatgc ctttcttctc tgctatctac
    17821 tttgctgccc aacatactga aggggttgct caagcaagct acctaggcat tcctctaggt
    17881 tctccaagta tgattttggt tgcctgtgct ggtgtccttt actatcttca atcgctcctt
    17941 tcacttcacg gagtagaaga tgaaatgcaa agagaacaaa tcaagaaaat gatttacatg
    18001 agcccactca tgatcgtcgt cttctccctc ttctcaccag ctagtgtcac actttactgg
    18061 gttgtcggtg gtttcatgat gattctccag caatttatcg tcaactatat cgttcgtcca
    18121 aaacttcgca aaaaagtcca tgaagaactg gcaaagaacc catcaaaagc aagtgctttc
    18181 tcaacaccaa gtggacgaaa agacgttacc cctgaacaac caactgctat tacaagcaag
    18241 aaaaaacaca aaaatcgcaa cgctggaaaa caacgttcga gataagaaga aaaaggctta
    18301 gctgatttgc taagtctttt tgcaagtcaa aagcccgatg tttccatcag gcttcttttc
    18361 tatccatgta cacgtttcat ataatcttga taactttcgg tatccatgag ttttttagcg
    18421 ttcttgacgc gatctgctgt tggtggtttg actccttcga gggaatatgg aattccaagt
    18481 tcacgccact tgaactcacc catggtgtga taaggtagaa tttcaaactt atcaacattt
    18541 ttgagggtct tgacgaactt accaagttca atcaagtcat catctctgtc tgtcaatcct
    18601 ggaactagca cgtggcgaat ccagacaggt tttccaatat ctgatagata ctgggcacaa
    18661 gccaagatat ttttattggt ttggctagtg acaatcttgt gctgttcttc gttgatttcc
    18721 ttgatatcca aaagaaccaa gtcagtgaca gccatgagtt tgtcaaactt ctcaaggtaa
    18781 cgcggtttat tacggaaagg aagagcacag gtgtccaagg tacagtggat tccttgttcc
    18841 ttagccttgg tgaagagagc aatcaggaaa tcaatctgca agagagcttc tcctccactg
    18901 actgtaatcc cacccttatt tccccagaaa ccacggtagc gcaaggcctc tgtcaagaca
    18961 tcatctaccg tccgttcacg tgacttattg gactccatag cccaagtgtc tgggttgtgg
    19021 caatactggc aacgcatgtg acagccctgc aaaaagacaa taaagcgaat accaggccca
    19081 tctactgacc caaagctttc tgtcgaatgc accatccctg tcacttgtcc ataatcaatt
    19141 gtttcttcag acatatcgtt accttccttg aaaacgtttt atagttttat tatatcacga
    19201 cttgaaggaa aaacaagatt tttgcctatt ttttagaaag caatctgttt ttcaatttca
    19261 ttttcaatct attatcagat tattcttcaa aatcgaagcc atataaggtt gcaaaatagt
    19321 cctcagggcg ctctgcacgg cggatttgac gggctttacc ttcttcggta tagaggattt
    19381 ccgcagaacg taatttggca ttatactggt agcccattga aaatccgtgg gcacctgtat
    19441 catgaattac cagcaaatca ccgatttctg tatgaggcag ttcgcgattg actgcaaatt
    19501 tatcattgtt ttcacagagt gaaccaacca catctaccgc ttcagctggt ccatctggat
    19561 gggtcacgtt gctaatatga tggtaagctc cgtacatagc tggacgcatg aggttgactg
    19621 ctgaggcatc tacacctaga taggtacggt aggtttcctt cttatgagtg actcttgtga
    19681 ctagagcacc gtgaggtgcc agcataaaac gacccaattc ggtgaaaatc ttgacctgac
    19741 caagacctgc tgacgtaaga acttcttcat acaccttacg aactccctca ccaatcaagg
    19801 cgatatcgtt cggctcctgg tctggacgat aattaacacc aataccgcca gaaagattga
    19861 taaagtctaa cgaaatgccc aacttttcct tgatttcaac agccagttca aagagctgac
    19921 gagccaactc tggataatag agatgggtca cggtattgga cgctaggaag gagtgaagcc
    19981 caaaagtctt ggctcctttt tccttcaaga tagcaaaggc ttcaaagagc tggtccttgg
    20041 tcatgccaaa cttagcctcc ccaggattgt ccataatgtc tgtccccagt tcaaaaacgc
    20101 ctccaggatt ataacgacaa gagatgattt ctggaatgcc tgctactctc tccagatgtt
    20161 caatatcttc aaaggcatcc aagttaatgg tcgcacccaa ttcacgtgca taggcgtatt
    20221 ccttgtctgg cgtgttgttg gaagagaaca taatctcaga acccagaaag tccagtttat
    20281 ggctcatcaa aagctctaca taactagagc agtccacacc acaaccttct tcttggagaa
    20341 ttttcaaaat agctggagtt ggagtagcct taactgcaaa atattcctta aagcccttgt
    20401 tccacgaaaa agcttggttg acagctcttg ccttctcacg aatccccttc tcatcataca
    20461 agtgaaaggg agtcgggaac tcggcaacaa tcgcttctaa ctcttctcta ttgataaatg
    20521 gtgttttcat aagcttcctt ctattctgtt tgcaaagaaa ggctgggaca atcgttccaa
    20581 cctttagttc tatctcttat ttatcttcgt caaagatatt gccaacctca acatcgatgg
    20641 cttggttctt gacatcaata ttggttacct tcaagagtga cttgtagtca aactgctttt
    20701 cacgttcttc ttgggcattg tccgcaaaga ccgctacacc tgccagttct gagtcgaact
    20761 cgcgcaagag actaatcata ccgttgaccg ttccgccacc tttcaagaag tcatccacaa
    20821 tcaagacacg gctgcctgcc ttaagactac gttttgaaag gaacattttc tcgatacggt
    20881 caccacttga acctgaaaca tagttgacgc taacagttga accttcggta attttcaggt
    20941 cacggcgcac aatgacaaaa gagacattga ggacattggc aactgcattt gcaagtggca
    21001 cacccttagt tgctacggtc ataaccgcgt caattttttg gtccataaag cttttggcaa
    21061 taatacgacc aatatttttc aagatggctg gtgtgctaag caaatcagac agatagatat
    21121 aaccacctgg caagatacgg tcactttctg acaacttggt acgcaagtct tcaaccattt
    21181 ccttagcatc ctgactcgaa atagacggtg tgaagatgac acctccgcca gccccagtca
    21241 ctgtctggat atgaccgatt tcaatttcct caaaggcgcg tttgataatg acgatatctt
    21301 ctgagatgga tgatttagca gactcatact tttcagcaaa agtattgaga ctagttagtt
    21361 tataaggatt attaatcaaa tagttggaaa tgacaaccat ccgatcactt cttcttaatt
    21421 tcatttctat ttcctattcc atttttattc aaaaaatcaa aaagcaaact agaaagctgg
    21481 tcgctggtgg ttcaaaacac tgctttgaga ttgtcgatag aactgacaaa ccctgtaata
    21541 tacctgcata tatacatacg acaaggcgat actaccctag tttgaagaga ttttcgaaga
    21601 gtattcattt ttgtctttta cttattatac catattcaca taaaaaaacg aacattctta
    21661 tcctaaaaaa tgctcatttt tcttaaatta ttaatctaaa tctggtttat agaaggaacg
    21721 attatccata gcgaagattt tattggacat ctctccttta tccaccaaag ccagagctgt
    21781 tgacatcatc atcatgcttg catccagatt gtcaatcata tggataatct ctgcctccat
    21841 aatacgtgga cggactggac ttccatattc aagcaagccg tggtgactga ggatgacatg
    21901 acgaagcaaa acgacttctt ccttggtatc gtcgatgccg agttccataa ctgtcttggt
    21961 aatttcgcta tcaatgagag cgatatgtcc aagaagattg cctcgcactg tgtactctgt
    22021 ctggtctggc cccgtcaact cgatgacctt agctaagtca tgcaacataa tccccgcata
    22081 gagcaggctc ttattgagct gaggataaac ttcgctaata gcgtctgcca aacgcaccat
    22141 ggtcgccgta tgataggcca agcccgtttc aaaggcatgg tggttggtct tggcagctgg
    22201 ataggagtag aattccttat catacttggt gtagagattt cggacaatcc gttgccagac
    22261 aggattttca attttgaaaa tcatttgcga catgtagtca cgaatttcct tgacatcaac
    22321 tggtgacttg accttgaaat cagctgggtc attgggttca ccagcttgag gcaggcggag
    22381 agtaatttga ttgacttgag gggtattgtt ataaacttct cggcgtcctt tcatgtggac
    22441 aaccttacct gcggtaaagg cctcaatgtt atgaggttgg gcatcccaga gcttcccatc
    22501 aatctcgcca ctatcatctt ggaaggtaaa ggctaggtag tttttcccag ctcgagtttg
    22561 cctcaggtca gctgatttga ttaggtaaaa gccttcaaat aactcatctt ttttcatgtg
    22621 actaatcttc atattcttcc tcattttctt gaaaatggag tagatcaagc gcaggctcac
    22681 cttctgacaa ctcaatgtga cggagcgtcc gctcgatagc tatggtacga cggtttaata
    22741 attcatcaat attgccagag gcatgttgga gatgtttttg tgccttgacc agaataccac
    22801 caaacttgcc aaactcggtc ttgacactgg caagagtctt gctgatatgg tcggcactct
    22861 tttggatatt aagggtcttg aaaccaactg atagggagtt aagaagggct gatagggtac
    22921 ttggtcctgc aacaataatc tgttcttccc gtctcaaatc atcaaagaag accggattgc
    22981 ggacgatttc tgagtagaga ccttctgtcg gaacaaacaa gattccaaaa ttggttgtcc
    23041 gaggcggtgc tatgtacttg ctcttgatat ccttggcaaa gcgcttgacg cttgctagga
    23101 gtgacttacg acagcgttca atctcatcct tgtcacctgt ctcataggct tcttccaagc
    23161 ggtaataatc tgccagtgga aacttagagt caattggaag atagacgtat tcttggtcgc
    23221 cttgtccggg taacttgatg gcatactcca ctcgttcact agagttttca accgttgcgt
    23281 attctcgttc gtactgggca ggtgtcatga tgtcttcaat aatttgcccc agttgcaatt
    23341 ctcccagaat ccctcgcgtc ttggttccag agagaacctt gttaagagct ccgacatcac
    23401 gggcaactgt ctgcatttct ccaagaccac gattgacaga ctccagttgt ttagaaactg
    23461 tctcaaagga agcctgtaag cgtgtctgca aggtcttttc tagtttttcc tcgaccgtct
    23521 ggcgcatttg ttccaaacgt tgctcatttg attcctgcaa ggcttggaga cgttggtctg
    23581 tcttgtctct agtttggagg agattatctg tcatttcttg acggacttgg gtcaggcctt
    23641 ggtgcaattc aatccgcact tcttgcaaac ggtcgctgac agccacttcc aaatcttttt
    23701 ggtctaactg gctggcttgt ctggcttggt caaagcggta atccaactgg tctgacaaat
    23761 gatctgcctg atcctccaag ctcttactta agtgtttctc ctgcctatcc tgcctttgcc
    23821 aaatcagaaa gagaccagct aggttggcaa ttaataatag aataagtaaa ctttccatcc
    23881 tacctcctat ccttgctatg cagtacgacc acataaccat ctgggcaagt taccgacact
    23941 tccctatcta tatattcgtt agaagcgtac acttttttaa agaaaaaatt ttcctctgtc
    24001 aactcatact tggctccaag aatagtcagc tggctatccc gaactggcat aaaggctaga
    24061 tagtcgtagt ctgaacgagg ttctagctga ctggtccctt ctggacaata agtaatcaag
    24121 ttttgcccat cctcaatttc tatttgatgc atatagggtg ccaacttagg attgctaggc
    24181 agaaagacat tggccaacat atggtcaata cggccaccca aggcaccgaa aatagtaacc
    24241 tgagcctgag gattttgttc aaagatggtt aagagagcca attccaaatc ggtatcatct
    24301 ttttctggtc gtgcttggac aaaatgctgg gcacgttttt gaatcacctg tcgctcttct
    24361 tcggtcacag aatcaaaatc cccaactgct agagcaagag gcaagtcttc ttccaagacc
    24421 cagagcgagc ctcgatccac cccaacaaaa gcatcaaaat ctgtccgata atgaccgcgg
    24481 tctccgcctg caaaaacggc aaccctagtc cagttgtttt ctgagagttt gtactcgctc
    24541 attgacatct cccttaaaga cataggaacc tgctacaaaa acggtcgcac cagcttcttt
    24601 agcttgggca atagtttggt catcaatccc accatccact tcgatttcaa agttcaaacc
    24661 tttttcctca cgaagagcaa ccaactcacg aaccttatcc atggtttctg gcagaaaggc
    24721 ttgcccacca aaacctggat taaccgtcat gactaaaact tggtcaacta gatgaaggac
    24781 gtgcttgatg gcttcaactg atgtgccagg attgataacg actgaaggct taactccgag
    24841 tgaacgaatt ttttggaggg cgccatgaat atgaggcgtt gcttctacat ggatactgat
    24901 gatgtctgca cctgcgcgcg caaagtcttc cagatggtgt tcaggattcg ccaccatcaa
    24961 gtgacagtca aagaccatct tactatgtgg acgaagtgct tcaaccacac cggcaccaaa
    25021 actgatttgt ggcacaaaat gactgtccat gatatcgata tgggcatatt ctgccccagt
    25081 tgcttctaga cgtttgattt cacgttcaaa gttggcataa tctgctgcca gaattgacgg
    25141 agcaatcttg tattgagaca taggtttctc cttattttgg aatttttttg ctgacttttt
    25201 tatgggtttc tctacgattt tcaatttcac taaggaattg caggtaattg tcaaaacgga
    25261 aggttgcaat aacaccctct tcaacagctg gtttgacggc acaagacggc tcatgggtat
    25321 gggtacaagt acgaaactta caatctcggc tgacagtagc aatctctggg aaagcctgat
    25381 tgaggtcttc agcccttgat acttcatagt ccagagatga aaagcctggt gtgtctgcga
    25441 ttttaccccc attgaggttg aaaaaactaa cagctcgagt agtatggcgc ccacgaccca
    25501 gactgtctga gatttctcct gtttcaagat tgaggtctgg tgcgattttg ttgagaagag
    25561 ttgacttccc aacacctgtc tgccccataa agaccgtaac cttgcctgtt aacaaagaca
    25621 ggagttcctc tttactggtc acaaagtcat agccgatgtc accataggtc tgctggtaaa
    25681 aatccagttc tcccctatct tccaacaaat ccattttgga aatatagaca atgggatgga
    25741 tgcccttgtg ctccaaaaga accaagaaac gatccagcaa attgctgtta aaatcaggtt
    25801 ccttgacgga catgattact acagcttgat cgatattgac aataggcgga cgaaccagac
    25861 tgtttttccg ttcgtgaatt tttaggatat agccttctga attttcctcg gcagagaaat
    25921 ctacccagtc cccaacataa ggggtatggc cttttttacg gaaattccca cgcgcgcgtg
    25981 tttgataaac ctggccatca ctctccacat agtagaaacc tgccaaggct ttaatgattt
    26041 gtccctgcat cttagagtcc ttgcccttta agtgcgtctg ctagacaggc aaattctgcc
    26101 aagctgagag cttccccacg cacacttggt gacaagcctg cctggtccaa agccttggtc
    26161 agcttgtcct tgacctcttc agtcttacca aagtaacctg tcaagttatt ccacaaggtc
    26221 ttacggcgat gggtaaaact agccttggaa accttaaaga agaagttctc atcttccact
    26281 gctacagctg gctctggacg gcgcaccatt ttcaagatgg ctgaatccac atttggcgct
    26341 ggcacaaaga ccgtacgagg cacgataaag gcaaccttgg ctgtcatgta atactgcacg
    26401 gcgatagaca agctaccgta agccttggtg ttaggctggg ctgaaatgcg gtccgctact
    26461 tctttctgca tcatgaccac aaactcacta aaaggaatcc cactctcaat caagtgcatg
    26521 agaataggcg tcgtgatgta gtaaggcaaa ttagccacta ccttgattgg caggttagga
    26581 tttttaaaat tttggatatg ttgcgccaaa tcaaccttga gaatatcttc gttaactaca
    26641 gtcacattat caaaatcacg cagggtatct gccaaaattg gcaccaaacg gtggtcaatc
    26701 tcaaaagcca tgacttgggc tgcacgctca gccagaaact ccgtcaaggc accaatacct
    26761 ggcccgattt cgatgacatt gacctggtca tcaatttcag ccgtatccac aattttttga
    26821 aggatattgg tatccgtcag gaaattttgc ccgaaggact ttttaaaggt aaaaccgtga
    26881 cgctccagca ctgccttggt cacgctataa tctgcaattc tcatctattc tcttttctat
    26941 tatctgttac tactattgta acacattccc cttgtatttt gggaatgaaa atcataccaa
    27001 aaaactatct ttcctcatga agaaagatag tcttggaaat ctttaaaagt ggccttaaca
    27061 gttctctcga aacatgcgtt caaaaaaatc agcttcttga taacttcgat tttcgggtag
    27121 ggtcaaatcc acatcctcct ccacatcatc cactgtcagt tcaaccgtct gaggtaaatt
    27181 tttcaaacga taatgctttt gcctttgccc ttcaatacta gcaaaatcaa gcaaatagcc
    27241 atgtactcgg atttgaacca aaaggagatg attggtcata ttttccatga tgaggatatc
    27301 ttctaaacca cctctggcac taggataatc agatactctg cgaatacgga ttttttcaca
    27361 catgagcaag tttcctcata tcaatgattt catcagcctt attccagact aggggattat
    27421 gggtcgcaat gatgatcatt cgattttcat ctttcaaatc caccaagaga ttcataacct
    27481 cctctgaatt ttcaggatca agagctgctg ttggttcatc tgccaaaatc aagggtggat
    27541 ttttcaaaat agtcttagca agggcaactc gttgggcctc tcccccagat aaagtataaa
    27601 ttttttgttc taaatccaaa taccctagat taactttttc taaagccccc acttgccttt
    27661 ccaaacgttc tacttttgag attttctgac caacaaaacc caaatccaaa ttttctttga
    27721 ttgattggtt ttctaagagg ccaaaatttt gaaagagata gcccatctga tctcgaaaat
    27781 actcacgagt gggaatggtt tttaaatctt tcccctgata gagaaccctt cctccatcta
    27841 tcttttctag ctttcccaag atattcagca gcgtcgtctt tccgcttcca ctctttccga
    27901 ttaaggcata aaccttgccc ttctccagct tgagattcaa accagagaaa atcgcgcggt
    27961 cattaaattt cttttccaat ccttgaatat caatcatctt attttccttt caatactgcc
    28021 atgctaccag cttcttcttt tttgtcctgc cttactaaaa tcaagagggc gttaagcgta
    28081 aacaaagcta caactagagc gctaatcaaa ccatctcttg tcaaaatact agataggact
    28141 aggccaagca agagaactcc tccttgcgcc agtaaatact tgccatgaag ctcatagatt
    28201 gtcataccag caagacgttt aatcataagc tcccgtctga actgctcaaa atagagaaga
    28261 ttcatggaat caaataacaa gatagccgta gacagggtca aaatcgtccc aataaggaga
    28321 gagtaaaact ccacccgttt gtcatttagt accttggcaa aaaatagctg gctttttacc
    28381 aagtaagaaa ccttgtgata cagaccttgc tcttttagag ctgtgatgct gtctccatat
    28441 cgatctagtt tcaaggcact attagccgta gttccccaca acatccctgc aacaggtctt
    28501 gttccagtcg cttgcggcgt tagaactaca ataatcggat cttttaggta ctggcgtgtt
    28561 gtcttgtacc catcattata gaggaaacgt tcctgtcctg tttctgtaaa agctagcctt
    28621 acctgtggga agtagtgttt ctgactcgtc acttctacac tttcagatga aaagttttgc
    28681 aggtaatctg taaacaatcc ttggtagtag gcagactgct ctcgcaagct ctcaggcaag
    28741 atcagcccaa actctccctc agacaagttt tgcatcttgt ccataaactc tgaagaaacg
    28801 gtaatctttt cttctatcag atagcgtggt gagacataga taacattccc tgacggtgtg
    28861 tagtcactga gacgattacc atctaggtcc acttctgctc catctgagaa attgtcaaca
    28921 ttgctcataa tataaaaaga gtctgtattg gctaaccgtt cttcagtaaa tgtctgccac
    28981 tcacgattat ccttacgcgt tccttcttca tcggcaaatg cactagacca accaaaggat
    29041 agacggtaac ggtctgagta ctggctccat ttattgctag ctctctccat ttcctgcatt
    29101 tcacggtagt ggggtaggag agctgtcgca ctcgatccga ccaccaatac agctaagagt
    29161 tgccccacca tcatcaatgt catcatacgt ttgagaggga gtttcccttt caatagatcc
    29221 accagactat tttcctgtaa accaagtaga tagacgacac ttagtaaggt agaaatccct
    29281 gccaagatca atccataaag tagaagagca atgatgacca gttgcaccgt tgccataaac
    29341 aaggcacctt gataccagag aatccccaat cccaaaatac tggataccag cactgagcag
    29401 ataagctgtc tcacatcttc taacactggt ctgagagcaa ctccaaacaa gctctcacca
    29461 gctattaagc gaatccctgc ctgacgaagg gatttgatcc gataaatcag ggtcagactc
    29521 ataaaggtca gcagaaaaat agctaaactc agtagcacca taggggtggc cgtcaatagt
    29581 aagactatcg aaaatggatc ttctccattc gaaacaaagc cttgatagcc aagttctttc
    29641 aaggtggtct gtaagctcac tccatccaaa cttcctgaca caatcaagta agaccctaat
    29701 aaatcactcg tttctgcact ctccttggag gaaatggtca gaccttctgg aagctctccc
    29761 tcaccataag tggcataggt aaagagtgtt tctccagctt catttggctc aacgattcga
    29821 cgggcgatga gactattatg ctctaccgcc aagtgactca aagcctctgt caccttttcg
    29881 cgactagcta ctggttgatt tatcatcatt cctgtcacat caacagaagg atagttacca
    29941 aataaaatct cttctcgatg gtcaacggaa gttatcacca tataaagcga taccaatacc
    30001 attgaaatca aaataaataa acgtttcatc atttcctcca aaaagttact tggtcactta
    30061 gtgagaaggt gaccacagtc actgcgatgt taagaagctt attaagttct ttctgctagc
    30121 aacatcttca aatatattat atccgttttt tcaagaacta tcaaggacat caatgtcctt
    30181 aaaatctaac tatttcatat tatttttagc ttatatctaa aaaaacagtt ctaaagacga
    30241 actttagaac tgttttgata cgaggacaag gacatttttg tcactgattc actatgttta
    30301 taaatatttt ttgacatcct cttgccattc ctctgtcaat ttctttacta gaaatccatc
    30361 tccaattagc ttcgcaaatg ttttagaaga ctgataaaat gattcagcta aactaaaatc
    30421 tctttctact cttaatgctt gcttccacaa aaacataaat acaagaggtt tcttttgata
    30481 atcttgagtt ttatccataa tttcttgtaa acacgataga taatcattaa aacaatcagt
    30541 tactcctacc ttttcaagac aacacattcc tgaaataaga gtatctctaa ttataaagta
    30601 attctctatt tgagttacct tcttctgttc caaaagaaag agcattagct tttcgatttg
    30661 atttataaat ttggcaagat cttttctaat gagcatttga taaaaaaaca aacgaatcaa
    30721 caacatatca tttgttgaat aaacttcttt ttctatgatc tgagacatat gttcctcaag
    30781 tattaactct gcatattcag gccttctagt tctagaagta tccaaacacg cctgcaatac
    30841 ttcacatgct aattgttctt ctttaggaag attatcaaag tatgttttat aaatctcttc
    30901 caaacacgct tccttcttat cgtactcctc ttctttacca tagattggtt cacgtaaaat
    30961 ctggtatttc aattctaaat aagcagaagg aagagctgaa aaatccggca taaggctata
    31021 aactggaact cctagccggc gagcaatata gtctaacttg ggcaaactcg gttgcgaagc
    31081 tccactttca attcgcgaca gttgacgaac tgtcagttct tgctcatctc cacaaaaatc
    31141 tggacgacta attccttttt ctatccgaat ctctttaatt ttttgaccaa ttaacatcag
    31201 acgcctcctc ttctacctgc ttcgaaaaat actgtgaaac ttaaaaaatt gtaacggtca
    31261 catctttcct actcataaga tttcatagct tcttccactt ctgccaaagt aaccccaaac
    31321 aactctaggc gtttgaggag ttgcttgccg ttagaatagc cgattcggag agtctctccg
    31381 agatattctc tacgcttaca gctgtctgcc cctgctagaa aaccaaggcg aatcaagtcg
    31441 ctacgactaa tgtcaaactg actctcatgt tcaaattgtt ctgtcacctg agctagagcc
    31501 gttttcaggt cttcatagct ggcatgctca attcccagag aacgcccctt ggtcttggac
    31561 ttgggaacag cttcatctcg cttgagaaag gcatgctgaa ctgttggaat gaccatcatg
    31621 atcatgcgcc gaatccgttc cccattaaaa tctgggtctg taaagacaat gactccatga
    31681 cgttggtgca ggcgctgaat ccgctctata tcctgagcat tgatggcaga acctcgagtc
    31741 tcataggtct ccacatcgaa ataacgtttg agattgaccg tatcatcacg cccttcaacc
    31801 acgataactt gagaaatttt ctctttcatt acttgctgtc cagtccaaaa attcgttctg
    31861 catttgcagt cgttgctacc gccagctctt ctgtcgtcat accacgcaag tcagcgataa
    31921 agtcgaccac atagcgagta taggctgttt tattttcacg accacgcttg ggtacaggtg
    31981 ctaagtaagg cgcatctgtt tccaccaaca tcttgtccaa aggtaactct ttagctgctt
    32041 cttggaggtc agttgccttc ttaaaagtca ccactcctga gaaggaaatg gtcataccaa
    32101 gatccacaaa cttctctgcc cactcaagcg tccctgaaaa tgaatgcatg ataccaccac
    32161 gaggaccaac gccctcactc ttgataatct cataggtatc ttccagcgca tcacgggtat
    32221 ggacaacaaa aggcaaatcc aagtccttag atagctgaat ctgacggcga aaaacctgct
    32281 cctgcacctc tttgggcgct gtcatccaat ggtaatctaa gccaatttca cctaaagcca
    32341 caaccttgga atgttttaac ttatccaaca agtaagcctc aacttcctct gtataagtac
    32401 cagcttctgt aggatgccaa ccaatagtcg catagagctg ctcatactca tctaccaact
    32461 ccaaggcatg ctcaatcgtc ggtttatcaa aaccaacaat attcatctgt gtcacaccca
    32521 tctcagcagc caaggcaatt tcttctgcct cacgacctgc aaattcttct acattcaagt
    32581 gtgtatgtgt atcaaaaatc atctcttcta acctcgtttt ctatcttcta ttataccaaa
    32641 aaagaggagg ggcacctaat ttttcggttt cccctcctct cttcaataga gagctattct
    32701 gctatctttt ctatccgata ttgcccatct cctattccac agttagagac agaagagatt
    32761 ggctacattg atagaatatt cctattcagc taaagtatgt gattatcttg ggttctcact
    32821 tttctcaccc atcactagga ttgatgactt ctcaaaatcc cccttataat ctaactttaa
    32881 gtagagaata gaattttcta gctgttctat tttctacgtc tagtctttct tcctagcaaa
    32941 gcaaaaccac ttgttgccac cactagacca agactagcaa gtgctgcact tgatgctgaa
    33001 cctgtgttag gtaattgttt gccagcaggt gactggtagg taacatcttg cattgttcct
    33061 gcagttgctt gcggtgcact tgttgtctta ggagcagcat taggagccac tggtctctta
    33121 ggaactgtaa ccatggcaat ggcttgcact ccaatcgttt ctgcttcctt cacatcttcc
    33181 atagttttcg cattttcaat ctctttgaga gctgcttgtt tttctgcttc cactcttgct
    33241 aaaagttctg ctttttcttt atcagaaagc ggtgcgcctt tgatttcatc ctgcttgtcc
    33301 ttggctgctt tttcaatggc atttttagct gattcttttt cagtagctaa ttgcttagaa
    33361 gcttgtgatt gagctgagtt ttgtgaacct gtgtgagctg atccacccgc ctggctacca
    33421 ccgccttcat tcgcattagc atctgagctt ccagtattgc ctcccttatc tccacctggt
    33481 gtattttcta gcttatactc tggtgtagct tcttgagtta cagattcacc tttcgcagat
    33541 tgttgaactg tatcttttcc tagaatcgtc actactgaac catctgggaa tgtgattgtt
    33601 gctgtaccat ctccagctac attgattgtc gctccgtcta atgctgaacc atttgcttgt
    33661 aaaatagcaa ccttaacttt ttcttcatct gttaaatgag ttatatcttt tacatctact
    33721 ctttgagcag gtgctttaac agttgatttt tcagcagtca gtttagtgct atcttgtgct
    33781 tttggatctt gtgctaccaa ttgatttcct ggaattgtaa tagctgaacc atctttaaag
    33841 acgattgttg ctgtaccatc tccacctact tcaactttat agtaaaataa aataataaca
    33901 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaaa cagaggtgta
    33961 ctattctagt ttcaatctac tgtaacatcg cttccgaatc cgtttgcatc tactgaactt
    34021 ttttacaaaa ataaaaatca gtagactcga gttcctactg atttttgata gaattcgttt
    34081 ggattatgca gccaaaactt tgcgtccttt acgacgacga gctgccaata cgcgacgacc
    34141 atttttagtt gacatacggt tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg
    34201 ttgataagta cgtttcacga tgaatacctc ctcatagatt ttgtattcgt ttagccggct
    34261 ataaagtgat cagttactaa acatacttta ctattctatc tgattctttc acttttgtca
    34321 atagctctag gcaaatgttt ccagcttttt cgaatgcaag ccctatctac gatactgttt
    34381 caagaaacga gtcatctttt cttggatttg ggctaactca tcaacacgag gaaggtaaac
    34441 gatacggaag tggtctggtt cctgccagtt aaagcctcga ccatgaacca agagaacctt
    34501 ttcctgcttc aagaaatcaa ggacaaactg ctcatcatca tcgatacggt acatattgcg
    34561 gtcgattttt gggaagatat agagccccgc cttgggttta acggcagaca aacctggaat
    34621 atcttgaatg gcattataga tgaaatttct ttgctcgtag attcgtccac caggaagaag
    34681 caattcatcg actgattggt gaccccccaa ggaagtttgt acgacttgtt gggccaaaac
    34741 gttagagcaa aggcgcatat tggacagcat attgagccct tcgatatagc ccttaacatg
    34801 agtcttaggg ccagacaaga ccatccaacc cacacggaaa cctgctatgc ggtgggattt
    34861 tgacagacca ttcatgctga cacagaagac atctggtgcc aagctcgcca caggcgtatg
    34921 cacatgtccg tccattacca tgcggtcata gatttcatcc gcaaagataa tcaaatcgtt
    34981 ttgacgggca atctcaataa tctccagcaa gagttcctta ggataaaggg ctccagttgg
    35041 gttattggga ttgataagga caattgcctt ggtattggaa gtgatttttg acttgatatc
    35101 gtcaatatct gggtaccatt ccacagcttc atcacagata tagtgaacgg catttcctcc
    35161 agctaggctg acagccgccg tccatagagg atagtctggc attggcacta agacctcatc
    35221 cccattgtcc aaaagcccct gcatggacat gacaatcaac tcactgacac catttccaag
    35281 gtaaatatca tcaatatcta cgtttgggaa tttcttcagc tggcaatact gcatgatggc
    35341 cttacgggct gagaaaatcc ctttggagtc agaatagcct tcactatcac gcgcattcat
    35401 aatcaagtca tgaatgacct cgtctggcgc tgtaaagcca aattctgctg gatttcctgt
    35461 attcagacgt aaaatctttt ctccattcgc tcgcatccgc atggcttctt ccaatacagg
    35521 accacggata tcataagcaa catgctctaa cttactagac ttgttatatt ctttcatctt
    35581 gtttcctcaa ttcatcagga tagtaacatt ataccactag aaagagaatg cgacaagttt
    35641 actgaaatgt gttactagtt tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa
    35701 cgtagagata gcaggtattt tgcaaagaca catcacatac caaaagataa acctagaaaa
    35761 aagcaagcct agaaaggagt cataaagaag attaaacatg aggaaaagcc cccatatcat
    35821 caagtaatcc caacgcccac ttcgttttgc aactagaaat aagattagat ggtagagtac
    35881 atgaaaaact aaaaatccaa taaaacttgg ataaagaagc ggtaccagac tctctaattg
    35941 acagatcatg atcacttcta ccaagactga aactaggata attccataat aaaggaaatt
    36001 tgatttttga aaagagtttt tcatcattct cccttccttc acttctttcc tgctatcctt
//
LOCUS       DAVLYR010000007        29745 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLYR010000007 DAVLYR010000000
VERSION     DAVLYR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.007"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1632
                     /gene="nanB"
                     /locus_tag="VYO63_000324"
     CDS             <1..1632
                     /gene="nanB"
                     /locus_tag="VYO63_000324"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1671306.1"
                     /translation="KTYTMYVNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEH
                     YLAKGSIGEISLFNKAISDQEVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGR
                     VLSSIDARYGGTHDSKSKINIATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKL
                     KNSQISGSASFIDSSIVEDKKSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLK
                     LKKNGDNDFRYTVRENGVVYDETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGS
                     LRERHNGKQVPMNVFYKDSLFKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGT
                     YLCPGQGLALKSSNRLIFATYTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVE
                     LRDGVIRTFFRTTTGKIAYMTSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDG
                     KEAVILSTPNSRSGRKGGQLVVGLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNH
                     HIGVLFEKYDSWSRNELHLSNVVQYIDLEINDLTK"
     gene            1644..2747
                     /locus_tag="VYO63_000325"
     CDS             1644..2747
                     /locus_tag="VYO63_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1671307.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQLSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            2920..3618
                     /locus_tag="VYO63_000326"
     CDS             2920..3618
                     /locus_tag="VYO63_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1671308.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            3637..5154
                     /locus_tag="VYO63_000327"
     CDS             3637..5154
                     /locus_tag="VYO63_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1671309.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            5279..6607
                     /locus_tag="VYO63_000328"
     CDS             5279..6607
                     /locus_tag="VYO63_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671310.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            6859..7725
                     /locus_tag="VYO63_000329"
     CDS             6859..7725
                     /locus_tag="VYO63_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1671311.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            7741..8580
                     /locus_tag="VYO63_000330"
     CDS             7741..8580
                     /locus_tag="VYO63_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1671312.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            8852..9304
                     /locus_tag="VYO63_000331"
     CDS             8852..9304
                     /locus_tag="VYO63_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1671313.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            9437..9745
                     /locus_tag="VYO63_000332"
     CDS             9437..9745
                     /locus_tag="VYO63_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671314.1"
                     /translation="MDGQGITVDEMAWIIRGIVNGLIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            9755..10054
                     /locus_tag="VYO63_000333"
     CDS             9755..10054
                     /locus_tag="VYO63_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671315.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKKSKRKKKKEKIDLFMVGTYESFTDKKENSLQRKMMEEQTWHKKE"
     gene            10036..10680
                     /locus_tag="VYO63_000334"
     CDS             10036..10680
                     /locus_tag="VYO63_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1671316.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            10849..11766
                     /locus_tag="VYO63_000335"
     CDS             10849..11766
                     /locus_tag="VYO63_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1671317.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            11784..12668
                     /locus_tag="VYO63_000336"
     CDS             11784..12668
                     /locus_tag="VYO63_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1671318.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(12684..13535)
                     /locus_tag="VYO63_000337"
     CDS             complement(12684..13535)
                     /locus_tag="VYO63_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1671319.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            13735..15792
                     /gene="pbp2b"
                     /locus_tag="VYO63_000338"
     CDS             13735..15792
                     /gene="pbp2b"
                     /locus_tag="VYO63_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1671320.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            15803..16399
                     /gene="recR"
                     /locus_tag="VYO63_000339"
     CDS             15803..16399
                     /gene="recR"
                     /locus_tag="VYO63_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1671321.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            16575..17618
                     /locus_tag="VYO63_000340"
     CDS             16575..17618
                     /locus_tag="VYO63_000340"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1671322.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            17702..19075
                     /locus_tag="VYO63_000341"
     CDS             17702..19075
                     /locus_tag="VYO63_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1671323.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELGITDLVERKDSLTFKVNFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     RLENEAK"
     gene            19062..19673
                     /locus_tag="VYO63_000342"
     CDS             19062..19673
                     /locus_tag="VYO63_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1671324.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKFLDGQFQEN
                     QEIADLQFFAIDQLPALSEKRITKEQIEILWQVYQGQREQYLD"
     gene            19764..20468
                     /locus_tag="VYO63_000343"
     CDS             19764..20468
                     /locus_tag="VYO63_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1671325.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            20687..22060
                     /gene="ftsA"
                     /locus_tag="VYO63_000344"
     CDS             20687..22060
                     /gene="ftsA"
                     /locus_tag="VYO63_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1671326.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            22077..23336
                     /gene="ftsZ"
                     /locus_tag="VYO63_000345"
     CDS             22077..23336
                     /gene="ftsZ"
                     /locus_tag="VYO63_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1671327.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            23341..24012
                     /locus_tag="VYO63_000346"
     CDS             23341..24012
                     /locus_tag="VYO63_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1671328.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            24022..24561
                     /locus_tag="VYO63_000347"
     CDS             24022..24561
                     /locus_tag="VYO63_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1671329.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            24561..24824
                     /locus_tag="VYO63_000348"
     CDS             24561..24824
                     /locus_tag="VYO63_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1671330.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            24821..25606
                     /locus_tag="VYO63_000349"
     CDS             24821..25606
                     /locus_tag="VYO63_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1671331.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            25615..26403
                     /gene="divIVA"
                     /locus_tag="VYO63_000350"
     CDS             25615..26403
                     /gene="divIVA"
                     /locus_tag="VYO63_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1671332.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            26751..29543
                     /locus_tag="VYO63_000351"
     CDS             26751..29543
                     /locus_tag="VYO63_000351"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1671333.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEYVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPTQDAVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT         9058 a         5324 c         6560 g         8803 t
ORIGIN      
        1 aaaacataca caatgtatgt taatggaata gaagtgttct ctgaaacagt tgatacattt
       61 ttgccaattt caaatataaa tggtatagat aaggcaacac taggagctgt taatcgtgaa
      121 ggtaaggaac attacctcgc aaaaggaagt attggtgaaa tcagtctatt taacaaagca
      181 attagtgatc aggaagtttc aaatattccc ttgtcaaatc catttcagtt aattttccaa
      241 tcaggagatt ctactcaagc taactatttt agaataccga cactatatac attaagtagt
      301 ggaagagttc tatcaagtat tgatgcacgt tatggtggga ctcatgattc taaaagtaag
      361 attaatattg ccacttctta tagtgatgat aatgggaaaa cgtggagtga gccaattttt
      421 gctatgaagt ttaatgacta tgaggagcag ttagtttact ggccacgaga taataaatta
      481 aagaatagtc aaattagtgg aagtgcttca ttcatagatt catccattgt tgaagataaa
      541 aaatctggga aaacgatatt actagctgat gttatgcctg cgggtattgg aaataataat
      601 gcaaataaag ccgactcagg ttttaaagaa ataaatggtc attattattt aaaactaaag
      661 aagaatggag ataacgattt ccgttataca gttagagaaa atggtgtcgt ttatgatgaa
      721 acaactaata aacctacaaa ttatactata aatgataagt atgaagtttt ggagggagga
      781 aagtctttaa cagtcgaaca atattcggtt gattttgata gtggctcttt aagagaaagg
      841 cataatggaa aacaggttcc tatgaatgtt ttctacaaag attcgttatt taaagtgact
      901 cctactaatt atatagcaat gacaactagt cagaatagag gagagagttg ggaacaattt
      961 aagttgttgc ctccgttctt aggagaaaaa cataatggaa cttacttgtg tcctggacaa
     1021 ggtttagcat taaaatcaag taacagattg atttttgcaa catatactag tggagaacta
     1081 acctatctca tttcggatga tagtggtcaa acatggaaga aatcctcagc ttcaattccg
     1141 tttaaaaatg caacagcaga agcacaaatg gttgaactga gagatggtgt gattagaaca
     1201 ttctttagaa ccactacagg taagatagct tatatgacta gtagagattc tggagaaaca
     1261 tggtcgaaag tttcgtatat tgatggaatt caacaaactt catatggcac acaagtatct
     1321 gcaattaaat actctcaatt aattgatgga aaagaagcag tcattttgag tacaccaaat
     1381 tctagaagtg gccgtaaggg aggccaatta gttgtcggtt tggtcaataa agaagatgat
     1441 agtattgatt ggagatacca ctatgatatt gatttgcctt cgtatggtta tgcctattct
     1501 gcgattacag aattgccaaa tcatcacata ggtgtactgt ttgaaaaata tgattcgtgg
     1561 tcgagaaatg aattgcattt aagcaatgta gttcagtata tagatttgga aattaatgat
     1621 ttaacaaaat aaaggagaaa aacatggtta aatacggtgt tgttggagca gggtattttg
     1681 gagctgaatt ggctcgctac atgcaaaaga atgatggagc agagattact cttctctatg
     1741 atccagataa tgcagaggcg attgcagaag aattgggagc aaaagtagca agttccttag
     1801 atgagttggt ttctagcgat gaagtagatt gtgttatcgt cgcaactcca aataatcttc
     1861 ataaggaacc ggttattaag gctgcacagc atggtaaaaa tgttttctgt gaaaaaccaa
     1921 ttgcgctttc ttatcaagat tgtcgcgaga tggtagatgc gtgtaaagaa aacaatgtaa
     1981 cctttatggc aggacatatt atgaatttct ttaatggtgt tcatcatgca aaagaactca
     2041 ttaatcaagg agttatcgga gacgttctat attgtcatac agctcgtaat ggttgggaag
     2101 aacaacaact gtcagtatca tggaaaaaaa ttcgtgaaaa atcaggtggt cacttgtatc
     2161 accacatcca tgaattggat tgcgttcaat tccttatggg gggcatgcct gaaactgtaa
     2221 ccatgacagg tggaaatgtg gcccatgaag gtgaacattt cggtgatgaa gatgatatga
     2281 tttttgtcaa tatggaattt tctaataagc gttttgcctt gttagaatgg ggttcagctt
     2341 atcgttgggg tgaacattat gtcttaatcc aaggaagcaa aggtgccatc cgcttagact
     2401 tattcaactg taaaggaact cttaagctag atgggcaaga aagctatttc ttgattcacg
     2461 aatcgcaaga agaagatgat gatcggactc gtatctatca tagtacagag atggatggag
     2521 caattgctta tggtaaacca ggtaaacgta ctccattatg gctatcatct gtcattgata
     2581 aagaaatgcg ctatctgcat gagattatgg aaggagctcc agtatcagaa gaatttgcaa
     2641 aacttttgac aggtgaagct gccctagaag caattgctac tgcagatgct tgtacccagt
     2701 ctatgtttga agatcgcaaa gtaaaattgt cagaaattgt aaaataaatt ttggtattct
     2761 cctattatag gtcgacttgc tcctctgaaa gtacttttag aggagctgtt tgacttggct
     2821 agtttttgaa actgaaatct attatactac aaactattga aagcgtttta attttaaggt
     2881 ataataatct catagaaata aagaaaagga ggaaagagga tgccacagat tagcaaagaa
     2941 gccttgattg agcaaatcaa agatggaatc atcgtttctt gtcaggctct tcctcatgaa
     3001 ccgctttata cagaagcggg aggggtgatt cccttgctgg tcaaagcggc tgagcaaggt
     3061 ggagcagtcg gtatccgagc aaacagtgtt cgcgatatca aggaaattaa ggaagtcact
     3121 aaacttccaa tcattgggat tatcaaacgt gattatccac ctcaggaacc cttcatcacg
     3181 gctactatga aagaagttga tgaattggca gaactggaca tcgaggtgat tgctctggat
     3241 tgtaccaagc gtgaacgcta cgatggtttg gaaattcaag agttcattcg tcaggttaag
     3301 gagaaatatc ctaatcagct tttgatggct gatactagta tcttcgaaga agggctagca
     3361 gctgtagaag caggaattga ctttgtcgga acaaccttat caggctacac atcctacagt
     3421 ccaaaagtag acggtccaga ttttgaattg attaagaaac tctgtgatgc cggtgtagat
     3481 gtcattgcag aaggaaaaat tcatacacca gaacaagcca aacaaatcct tgaatatgga
     3541 gtgcgaggca tcgttgttgg tggcgccatt actagaccaa aagagattac agaacgcttc
     3601 gttgctagtc ttaaataaga tgtgaggggg agttttatgt ttaaagtttt acaaaaagtt
     3661 ggaaaagctt ttatgttacc tatagctata cttcctgcag caggtctact tttggggatt
     3721 ggtggtgcac tttcaaaccc aaccacgata gcaacttatc caatactaga caatagtatt
     3781 tttcaatcaa tattccaagt aatgagctct gcaggagagg ttgtattcag taatttgtca
     3841 ctacttctct gtgtgggatt atgtattggc ttagcgaaac gagataaagg aaccgctgcg
     3901 ttagcaggag taactggtta cttagttatg actgcaacga tcaaagcttt ggtaaaactt
     3961 tttatggcag aaggatctgc aattgatact ggagttattg gagcattagt tgtcggaata
     4021 gttgccgtat atttgcacaa ccgatataac aatattcaat taccttccgc tttaggattc
     4081 tttggaggtt cacgcttcgt tcctattgtt acatcgttct cttctatctt gattggcttt
     4141 gtcttctttg ttatttggcc acctttccaa caacttcttg tttctacagg tggatatatt
     4201 tctcaggcgg gtccaattgg aacttttcta tatggatttt taatgagact ttctggagca
     4261 gtaggcttac atcatataat ttaccctatg ttttggtata ctgaacttgg tggtgttgaa
     4321 actgttgcag gacaaacagt ggttggagct caaaaaatat tttttgctca attagccgat
     4381 ccggcccatt ctggattatt tacagaagga acaaggtttt ttgcaggtcg tttctcaaca
     4441 atgatgttcg gtttaccggc tgcctgttta gcgatgtacc atagtgttcc taaaaatcgt
     4501 cgtaaaaaat acgcgggttt gttttttgga gttgctttaa catcttttat taccggtatt
     4561 acagaaccaa ttgaatttat gtttctattc gtcagtccgg ttctatatgt tgttcacgca
     4621 ttccttgatg gtgttagctt ctttattgca gacgtcttaa atatttcaat aggaaacaca
     4681 ttttcaggag gtgtaatcga tttcacttta tttggaattt tgcaggggaa cgctaagacg
     4741 aattgggttc ttcagattcc aattggactt atttggagtg ttttgtatta tattattttt
     4801 agatggttca ttactcaatt caacgttcta acgccaggac gaggagaaga agtagattct
     4861 aaagaaattt ctgaatccgc agattcaact tcaaatactg cagattattt aaaacaggat
     4921 agcctacaaa ttatcagagc cttgggtgga tcaaataata tagaagatgt agatgcttgt
     4981 gtgacacgtt tacgtgtagc tgtaaaagaa gttaatcaag ttgataaagc acttttaaaa
     5041 caaattggtg cagttgatgt cttagaagtg aagggtggca ttcaagcaat ctatggagca
     5101 aaagcaatct tatataaaaa tagtattaat gaaattttag gtgtagatga ttaagtactt
     5161 actgacttaa taaaaaacag aggagagtga tggatgagta ggatgaaatg aaatcgcata
     5221 caagaaataa agaactcatt atccaagttg gatacgctta ttacatagga gaatacaaat
     5281 gaaatttaga aaattagctt gtacagtact tgcgggtgct gcggttcttg gtcttgctgc
     5341 ttgtggcaat tctggcggaa gtaaagatgc tgccaaatca ggtggtgacg gtgccaaaac
     5401 agaaatcact tggtgggcat tcccagtatt tacccaagaa aaaactggtg acggtgttgg
     5461 aacttatgaa aaatcaatca tcgaagcgtt tgaaaaagca aacccagata taaaagtgaa
     5521 attggaaacc atcgacttca agtcaggtcc tgaaaaaatc acaacagcca tcgaagcagg
     5581 aacagctcca gacgtactct ttgatgcacc aggacgtatc atccaatacg gtaaaaacgg
     5641 taaattggct gagttgaatg acctcttcac agatgaattt gttaaagatg tcaacaatga
     5701 aaacatcgta caagcaagta aagctggaga caaggcttat atgtatccga ttagttctgc
     5761 cccattctac atggcaatga acaagaaaat gttagaagat gctggagtag caaaccttgt
     5821 aaaagaaggt tggacaactg atgattttga aaaagtattg aaagcactta aagacaaggg
     5881 ttacacacca ggttcattgt tcagttctgg tcaaggggga gaccaaggaa cacgtgcctt
     5941 tatctctaac ctttatagcg gttctgtaac agatgaaaaa gttagcaaat atacaactga
     6001 tgatcctaaa ttcgtcaaag gtcttgaaaa agcaactagc tggattaaag acaatttgat
     6061 caataatggt tcacaatttg acggtggggc agatatccaa aactttgcca atggtcaaac
     6121 atcttacaca atcctttggg caccagctca aaatggtatc caagctaaac ttttagaagc
     6181 aagtaaggta gaagtggtag aagtaccatt cccatcagac gaaggtaagc cagctcttga
     6241 ataccttgta aacgggtttg cagtattcaa caataaagac gacaagaaag tcgctgcatc
     6301 taagaaattc atccagttta tcgcagatga caaggagtgg ggacctaaag acgtagttcg
     6361 tacaggtgct ttcccagtcc gtacttcatt tggaaaactt tatgaagaca aacgcatgga
     6421 aacaatcagc ggctggactc aatactactc accatactac aacactattg atggatttgc
     6481 tgaaatgaga acactttggt tcccaatgtt gcaatctgta tcaaatggtg acgaaaaacc
     6541 agcagatgct ttgaaagcct tcactgaaaa agcgaacgaa acaatcaaaa aagctatgaa
     6601 acaatagtcc ttagttattc tataaaaagt agttttttaa agaacctaag agtgtatacc
     6661 cccttttccc tctacacaga tagtgtaaga aaagggggct tttgtttaaa atgtaagaaa
     6721 ctgtcacgaa attaaaatga agttcttaca taagcgaatc ataaaaaatt tcattttgat
     6781 tttaaaacag ttcaagaaag tcaaaaaatt attctatttg aaagagaggt gccgactgtg
     6841 aaagtcaata aaatccgtat gcgggaaaca gtgatttcct acgctttcct agcaccagta
     6901 ttattcttct ttgtcatctt tgtgttggct ccgatggtga tgggcttcat tacaagtttc
     6961 tttaactact caatgactaa atttgagttt gtaggcttgg ataactatat ccgtatgttt
     7021 aaagatcctg tctttacaaa atctctgatt aacacagtta ttttggttat tggatctgta
     7081 ccagttgttg ttctattctc actctttgta gcatctcaga cctatcatca aaatgtcatt
     7141 gccagatcct tctaccgttt cgtcttcttc cttcctgttg taacgggtag tgttgccgtg
     7201 acagttgttt ggaaatggat ttatgaccca ctatcaggga ttctaaactt tgtccttaag
     7261 tccagccaca tcatcagcca aaacatttct tggttgggag ataaaaactg ggcattgatg
     7321 gcgattatga ttattctctt gaccacttca gttggtcagc ccatcatcct ttatatcgct
     7381 gccatgggga atattgacaa ttcactggtt gaagcggcgc gtgttgatgg tgcaactgag
     7441 tttcaagttt tttggaagat taaatggcca agccttcttc caacaactct ttatattgca
     7501 atcatcacaa caattaactc attccagtgt tttgccttga ttcagctttt gacatctggt
     7561 ggtccaaact actcaacaag taccttgatg tactaccttt acgaaaaagc cttccaattg
     7621 acagaatacg gctatgccaa cacaattggt gtcttcttgg cagtcatgat tgctatcgta
     7681 agctttgttc aatttaaagt acttggaaac gacgtagaat actaaagaaa ggagacagct
     7741 atgcaatcta cagaaaaaaa accattaaca gcctttactg ttatttcaac aatcattttg
     7801 ctcttgttga ctgtgctgtt catctttcca ttctactgga ttttgacagg ggcattcaaa
     7861 tcacaacctg atacaattgt tattcctcct cagtggttcc ctaaaatgcc aaccatggaa
     7921 aacttccaac aactcatggt gcagaaccct gccttgcaat ggatgtggaa ctcagtattt
     7981 atctcattgg taaccatgtt cttagtttgt gcaacctcat ctctagcagg ttatgtattg
     8041 gctaaaaaac gtttctatgg tcaacgcatt ctatttgcta tctttatcgc tgctatggcg
     8101 cttccaaaac aagttgtcct tgtaccattg gtacgtatcg tcaacttcat gggaatccac
     8161 gatactctct gggcagttat cttgcctttg attggatggc cattcggtgt cttcctcatg
     8221 aaacaattta gtgaaaatat tcctacagag ttgcttgaat cagctaaaat cgacggttgt
     8281 ggtgagattc gtaccttctg gagtgtagcc ttcccgattg tgaaaccagg gtttgcagcc
     8341 cttgcaatct ttaccttcat caatacttgg aatgactact tcatgcaatt ggtaatgttg
     8401 acttcacgta acaatttgac catctcactt ggggttgcga ccatgcaggc tgaaatggca
     8461 accaactatg gtttgattat ggcaggagct gcccttgctg ctgttccaat cgtcacagtc
     8521 ttcctagtct tccaaaaatc cttcacacag ggtattacta tgggagcggt caaaggataa
     8581 tactctgaga aaatctcttc aaactacgtc agcgtcacct tgccatactt aagtattgcc
     8641 tgcggttagc ttcctagttt gttcttcaat tttcattgag tataggaaaa tcaatctatc
     8701 aagatacaga agtatatttt atagatttag agaatataga ggttataagt gtctacaaaa
     8761 tggagggtat gcagttactt tatgaagttt tgtcagacac ttataaactt aagaatggtt
     8821 ttagttaact atcagaaacg aaggaaagag tatgattttt gacgatttga aaaacatcac
     8881 cttttacaaa gggattcatc ctaatttaga caaggctatc gactatctct accaacatcg
     8941 taaggattct ttcgaattag gaaagtatga tattgatgga gataaagtct ttctagttgt
     9001 tcaggaaaat gtcctcaatc aagctgaaaa tgatcaattt gagtatcata agaactatgc
     9061 agatttgcat ttgctggtag aaggacatga atattcgagc tacggttcac gtatcaaaga
     9121 cgaggcagta gcattcgacg aagcgagtga cattggcttt gttcattgtc atgaacacta
     9181 cccactcttg ttgggttatc acaattttgc gattttcttc ccaggtgagc cacatcagcc
     9241 aaatggttat gcaggcatgg aagaaaaggt tcggaaatat ctctttaaaa ttttgattga
     9301 ttaaaaatag gatgaattgt ttttttgtaa agctttgaca atactctacc atgaaattga
     9361 tctttgtgag gtagggaaat gagaataaaa tatttaaaaa ttggtatctt ctaagtatgc
     9421 tgcaagagct agtttcttgg atggacaggg aattacagtt gatgagatgg cttggataat
     9481 taggggcatt gtgaatggat tgattggtag atacataaaa ttaggtactt atgcagccaa
     9541 gtatggtatt agtatggcgc gttcgatctt aagtagggca gctgcaactg cagcagcaag
     9601 agtaggatta tcgaccaaga tttctggatg gattttacga gtagctgtga atgtagctga
     9661 tgtatatggt aattttgcca acaatattgc tgcagcttgg gatgcacatg ataaaattcc
     9721 taacaatggt cgtataaact tttaaaatgc gagaatgaaa gcactttgta tttttttatt
     9781 gaatatgtta gcttggacag tgcttgcaat gataattcgt ggagggctag atggatttga
     9841 tgagcatact tggagtacta ttttaattgc ttcgctgttc ggggtatatg attataagcc
     9901 catagataaa aatagaaaaa agtccaaaag aaaaaagaaa aaagaaaaaa tagatttgtt
     9961 catggtaggg acttatgaaa gctttactga caaaaaagaa aacagtttac aaagaaaaat
    10021 gatggaggag caaacatggc acaaaaagga gtaagcctta tcaaggcagc atttgataca
    10081 gacaactttc tcatgcgttt tagtgagaaa gtcttggaca tcgtgacagc caatcttctt
    10141 tttgtcgtct cttgtttgcc catcgtgacg attggagtgg ctaaaatcag cctctacgag
    10201 accatgttcg aagttaagaa gagcagacgg gtgcctgttt ttaaaatcta tctaagatct
    10261 ttcaagcaaa atctgaaact aggtcttcag ctgggtttaa tggagttagg aattgtgttt
    10321 cttacccttt cagatctcta tcttttctgg ggtcaaacag ctctgccctt ccaattgctg
    10381 aaagccattt gtttaggtat tctgattttt cttactatcg tgatgctggc tagttaccct
    10441 atcgcggcac gttatgacct atcttggaaa gaaattcttc aaaaaggatt gatgttggct
    10501 agttttaact ttccttggtt cttcctcatg ttagccattc ttgtcctcat tgtgatggtt
    10561 ctttatctgt ccgccttcag tctactctta ggtggctcag tcttcttact ttttgggttt
    10621 ggactattgg tctttatcca gactggattg atggagaaaa ttttcgcaaa ataccaatag
    10681 gagccttatt tctgaaacta ctttcaaacg ctccaaacgc tattctataa gcgagaaact
    10741 aaaatctaag tgtctataag atattgaaag ccttttcggt aaagtgtata ctaaagaagt
    10801 aaagaaaaat ctgctttagc agaaaataaa aataatagga gaaaatctat gtcagatttg
    10861 aaaaaatacg aaggtgtcat tccagccttc tacgcatgtt atgatgaaca aggagaagta
    10921 agcccagaac gtacgcgtgc cttggttcaa tacttcattg ataaaggtgt tcaaggtctt
    10981 tatgtcaatg gttcttctgg tgaatgtatc taccaaagtg ttgaagaccg caagttaatc
    11041 ttggaagaag tcatggcggt agccaaaggt aaattgacca tcattgctca tgttgcctgc
    11101 aacaatacta aagatagtat ggaacttgct cgccatgctg aaagcttggg agtagatgct
    11161 attgcaacga ttccaccaat ttatttccgc ttgccagaat actcagttgc caaatactgg
    11221 aacgatatca gttctgcagc tccaaacaca gactacgtga tttacaacat tcctcaattg
    11281 gcaggggttg ctttgactcc aagcctttac acagaaatgt tgaaaaatcc tcgtgttatc
    11341 ggtgttaaga actcttctat gccagttcaa gatatccaaa cctttgtcag ccttggtggg
    11401 gaagaccata tcgtctttaa tggtcctgat gagcagttcc taggaggacg cctcatgggt
    11461 gctagggctg gtatcggtgg tacttatggt gctatgccag aactcttctt gaaactcaat
    11521 cagttgattg cggataagga cctagaaaca gcgcgtgaat tgcagtatgc tatcaacgca
    11581 atcattggta aactcacttc tgctcatgga aatatgtacg gtgtcattaa agaagtcttg
    11641 aaaatcaatg aaggcttgaa tattggatct gttcgttcac cattgacacc agtgactgaa
    11701 gaagatcgtc cagttgtaga agcggctgct gccttgattc gtgaaaccaa ggagcgcttc
    11761 ctctaatcta aaaggaggta tttatgacat actacgttgc aattgatatc ggtggaacca
    11821 acatcaagta tggtttggtt gatcaagagg ggcaacttct tgaatcgcat gaaatgccaa
    11881 ctgaggcgca taagggtgga cctcatatct tacaaaagac caaagatatc gtagctagtt
    11941 atttagaaaa aggcccagta gcaggtgttg ccatatcttc tgctgggatg gtggatccgg
    12001 ataagggtga gattttctat gctgggcctc aaatccctaa ctacgcaggc acccagttca
    12061 aaaaggaaat cgaagaaagc tttactattc cttgtgagat tgaaaatgat gtcaactgtg
    12121 caggtcttgc tgaggcagta tctggttcag gcaagggagc aagtgtgaca ctttgcttga
    12181 ccattggaac cggtatcggt ggttgcttga ttatggatag gaaagtcttc catggtttta
    12241 gcaattcagc ctgtgaagtc gggtatatgc atatgcagga tggagctttt caggatctcg
    12301 cttctacaac agctttagtg aaatatgtag ctgaagccca tggagaagat gttgatcagt
    12361 ggaatggccg acgtatcttt aaggaagcta ctgaaggaaa caaaatctgc atggaaggta
    12421 ttgaccgtat ggttgactat ctaggaaaag gtctggcaaa tatttgttat gtggccaatc
    12481 cagaagtggt tattcttggt ggtggtatca tggggcaaga ggctatcctc aaacctaaga
    12541 tccgcacagc cttgaaagag gcattggtac caagtttagc agaaaaaaca cgattagaat
    12601 ttgcccatca ccaaaataca gcagggatgt tgggtgcata ttatcatttt aagacaaaac
    12661 aatcctagtt tggctcagcc aaactaggat tttcttacac gtttttgtct acgatagccg
    12721 ttgagttttt tattttccca gtagctatta aagatttttt ccttgctttc gcgattgatt
    12781 tccaaaaagt aggcataaat caaatcgata aagaagagca taggaagttg agcggatatt
    12841 cgttggatgt aggagggttg gctgtgggtg gctacaagaa cagtctctgt ataggtctgg
    12901 ctatctttat tgggaacact tgtaaagagt acagtctttg cccccatctc cttggcatct
    12961 aatagactat ctaaaataga aggagttgag cctgaaagtg agaaacccag tactagacaa
    13021 ttttcatcca tgatgctggt tgtccaggca aagccgtctt ggtctgtcaa agcttcgcag
    13081 accacaccta gtcgcataaa acgtaatttc atttcacggg cgacgaggcc agaactccct
    13141 gttccaaaga agtagatacg ctcagcatct tcgattagct gggcaattcg ctctagttgg
    13201 atttcgtcaa tcaagtcttg tgtttgttcc ctcatattgc tataacttct gaggactcgt
    13261 ttggtcagtg gactatgctt ggagacttgg ttggcttgat tttctgcctg atgttggtat
    13321 tggaaaataa attctcggta gccagtaaag ccacactttt tagcaaagcg ggtcaaagca
    13381 gcttgagaaa tatgtaattt ttgggtgact tgttgagaag ataaatcatc tgtaatcgtt
    13441 tcagcttgca aaaaatagcg agcgatttct tgctctaggt ctgtcatttc ttcaaaatgt
    13501 gaatcaatga tagttgcgat atctggtttg tccataggga aagctccttt acatgggtca
    13561 tactggaaga ctagatcagg gaatagtcac acttcattat aacacataat ataaggatag
    13621 ataaataaaa acgcatctct gttttaaaaa cgaaaaaatc gaaaaagctt ctctcttttc
    13681 cataattttc tactcaaatt gtggtacaat taagagtaag attttaagtt agaaatgaga
    13741 ctgatttgta tgagaaaatt taacagccat tcgattccga ttcggcttaa tttattgttt
    13801 tcaatcgtca ttttactctt tatgaccatt attggtcgtt tgttgtatat gcaggttttg
    13861 aacaaggatt tttacgaaaa aaagctagcc tcagctagtc agaccaagat tacaagcagt
    13921 tcagcccgtg gggaaattta tgatgctagt ggaaaacctt tggtagaaaa tacgttaaag
    13981 caggttgttt cctttacgcg tagcaataaa atgacggcta cagacttaaa agaaacagct
    14041 aaaaagttac tgacttatgt gagcatcagt tctccaaatt tgacagaacg ccagctggcg
    14101 gattactatt tggctgatcc tgaaatctat aaaaaaacag tggaagctct cccaagtgag
    14161 aaacgcttgg attcagatgg caatcgtcta tccgaatcag aactgtataa caatgcggtc
    14221 gatagtgtcc aaacaagtca actaaactat acagaggatg aaaagaaaga aatctatctt
    14281 tttagtcagt taaatgctgt tggaaacttt gcgacaggaa ccattgctac agatcctcta
    14341 aatgattctc aggtggctgt tattgcctct atttcaaagg agatgcctgg cattagtatt
    14401 tctacttctt gggatagaaa ggttttggaa acttcccttt cttctatagt tgggagtgta
    14461 tccagtgaaa aagctggtct cccagcggaa gaagcagaag cctatcttaa aaaaggctat
    14521 tctctaaatg atcgtgtagg aacctcctat ttggaaaagc aatatgaaga gaccttacaa
    14581 ggaaaacgct cggtaaaaga aatccatctg gataaatatg gcaatatgga aagcgtggat
    14641 acaattgagg aaggtagtaa gggaaacaat atcaaactga ccattgattt ggctttccaa
    14701 gatagcgtgg atgctttact gaaaagttat ttcaattccg agctagaaaa tggtggagcc
    14761 aagtattctg aaggtgtcta tgcagtcgcc cttaacccaa aaacaggtgc ggttttgtct
    14821 atgtcaggga ttaaacatga cttgaaaacg ggagagttga cgcctgattc cttgggaacg
    14881 gtaaccaatg tctttgttcc aggttcggtt gtcaaggcgg cgaccatcag ctcaggttgg
    14941 gaaaatggag tcttgtcagg aaaccagacc ttgacagacc agtccattgt cttccaaggt
    15001 tcagctccca tcaattcttg gtatactcag gcttacggtt cattccctat cacagcggtc
    15061 caagctctgg agtattcatc aaatacctat atggtccaaa cagccttagg tcttatgggg
    15121 caaacctatc aacccaatat gtttgtcggc accagcaatc tagagtctgc tatggagaaa
    15181 ctgcgttcaa cctttggcga atatggcttg ggtactgcga caggaattga cctaccagat
    15241 gaatctactg gatttgttcc caaagagtat agctttgcta attacattac taatgccttt
    15301 gggcagtttg ataactatac gccgatgcag ttggctcagt atgtagcaac tattgcaaat
    15361 aatggtgttc gtgtggctcc tcgtattgtt gaaggcattt atggtaataa tgataaggga
    15421 ggactgggtg acttgattca gcaactgcaa ccgacagaga tgaataaggt caatatatcc
    15481 gactccgata tgagcatctt gcaccaaggt ttttatcagg ttgcccatgg tactagtgga
    15541 ttgacaactg gacgtgcctt ttcaaatggc gccttggtat ccattagcgg aaaaacaggt
    15601 acagccgaaa gctatgtggc agatggtcag caagcaacca ataccaatgc ggtggcctat
    15661 gccccatctg ataatcccca aatcgctgtt gcagtggtct ttcctcataa taccaatcta
    15721 acaaatggtg taggaccttc cattgcgcgt gacattatca atctgtatca aaaataccat
    15781 ccaatgaact agaaaggaaa ttatgcttta tccaacacct attgccaagt tgattgacag
    15841 ttattctaag ttaccaggta tcgggattaa gacggctacg cgtctggcct tttatacgat
    15901 tgggatgtct gctgatgatg tcaatgaatt tgcaaaaaat ctcctttctg ctaagagaga
    15961 attgacatat tgttctattt gtggacgttt gacagacgac gatccttgtt ctatctgtac
    16021 tgatccgact cgtgaccaga caacaatttt agttcttgag gatagtagag atgtggcagc
    16081 catggaaaat atccaagaat accatggact ctatcatgtc cttcatggcc tcatttctcc
    16141 tatgaatggt atcagtccgg acgatatcaa tctcaagagc cttatgactc gtcttatgga
    16201 tagtgaagtt tcagaagtga ttgtggcgac taatgctaca gcggatggtg aagcgacttc
    16261 catgtatctt tcacgtttgc tcaagccggc tggtatcaag gttacgcgtc tagcacgagg
    16321 tctcgctgtg ggagcggaca ttgagtatgc ggacgaagtg acactcttac gagcgattga
    16381 aaatcggaca gagttgtaag tgtaggcaaa tttacgaact ccattcattt ataaaaaatc
    16441 aaagaggctg aaaatcgttc ctatcggcct ctttttgtat agtgtgatga gtaggatcag
    16501 gttcaagttt taaaaaacca agcaaatatg atatactaaa gagcgagtat tctagtagaa
    16561 ttaggacaaa taatatgaaa caaacgatta ttcttttata tggtggacgg agtgcggaac
    16621 gcgaagtctc tgtcctttca gctgagagtg tcatgcgtgc ggtcaattac gaccgtttca
    16681 cagtcaagac tttctttatc agtcagtcag gtgactttat caaaacacag gaatttagtc
    16741 atgctccggg gcaagaagac cgtctcatga ccaatgaaac cattgattgg gataagaaag
    16801 ttgcaccaag tgctatctac gaagaaggtg cagtggtctt tccagtcctt cacgggccaa
    16861 tgggagaaga tggctctgtt caaggattct tggaagtttt gaaaatgcct tacgttggtt
    16921 gcaacatttt gtcatcaagt cttgccatgg ataaaatcac gactaagcgt gttctggaat
    16981 ctgctggtat tgcccaagtt ccttatgtgg ctatcgttga aggcgatgat gtgactgcta
    17041 aaatcgctga agtggaagaa aaattggctt atccagtctt cactaagccg tcaaacatgg
    17101 ggtctagtgt cggtatttct aagtctgaaa accaagaaga actccgtcaa gccttaaaac
    17161 ttgccttccg atatgacagc cgtgtcttgg ttgagcaagg agtgaatgcc cgtgaaattg
    17221 aggttggcct cttgggtaac tacgatgtca agagcacgct acctggagaa gttgtcaagg
    17281 acgttgcctt ttatgactac gatgccaagt atattgataa caagattact atggatattc
    17341 ctgccaaaat cagtgatgat gtggtggctg tcatgcgtca aaatgcagaa acagccttcc
    17401 gtgccattgg tggccttggt ctatctcgtt gcgatttctt ctatacagat aagggagaga
    17461 tttttctcaa cgagctcaat accatgccag gttttaccca gtggtctatg tacccactac
    17521 tttgggacaa tatggggatc agctacccag aactaatcga gcgtttggtt gaccttgcca
    17581 aggaaagttt tgacaagcgc gaagcgcatt tgatataaaa tgaaagagag ggtagaagcc
    17641 agaaccatca ctgcacggtg actagagttc tcggacttca gcccttttta aaggagtaga
    17701 aatgaaatta acaatccatg aaattgccca agttgttgga gccaaaaatg atatcagtat
    17761 ctttgaggac acccagttag aaaaagctga gtttgatagt cgtttgattg gaactggaga
    17821 tttatttgtg ccacttaaag gtgcgcgtga tggccatgac tttattgaaa cagcctttga
    17881 aaatggtgca gcagtaacct tgtctgagaa agaggtctca aatcatcctt acattctagt
    17941 agatgatgtt ttgacagcct ttcaatccct agcatcctac tatcttgaaa aaacgactgt
    18001 tgatgtcttt gctgttacag gttcaaatgg caagacaacg actaaggata tgttggcgca
    18061 tttactgtca acaagataca agacctacaa aacacaaggc aattacaata atgagattgg
    18121 ccttccttac acagttcttc atatgcctga aggaacagaa aagttggttt tggagatggg
    18181 acaggatcac ttgggagata ttcatctctt gtctgaattg gctcgtccaa aaacagccat
    18241 cgtgaccttg gttggagaag ctcatttggc ctttttcaaa gatcgttctg agattgctaa
    18301 aggaaaaatg caaattgcag acggaatggc ttcaggttcc ttgcttttag cgccggctga
    18361 ccctatcgta gaggactatt tgccaactga taaaaaggtg gttcgttttg gtcaaggagc
    18421 agagctggga attaccgact tggttgagcg caaagatagt ctgaccttca aggtcaattt
    18481 cttagagcaa gcccttgatt tgccagtaac tggcaagtac aatgcgacaa atgctatgat
    18541 tgcatcctat gttgccttgc aagaaggagt ttcagaggag caaattcgtt tggccttcca
    18601 acatcttgaa ttgacgcgta accgtaccga gtggaagaaa gcagccaatg gagcagatat
    18661 cctgtcagat gtttataatg ccaatccaac tgctatgaaa ctgattttag agactttctc
    18721 tgccattcca gccaatgaag gtggcaagaa aattgcagtg ttggcggata tgaaggagct
    18781 tggtgaccag tctgttcaac ttcataatca gatgattttg agcctctccc cagatgtgct
    18841 tgatatcgtt attttctatg gagaagatat tgctcagttg gctcaattgg ccagtcaaat
    18901 gtttccaatc ggccacgttt actacttcaa gaaaacagaa gaccaggatc aatttgaaga
    18961 cctagtcaag caggttaagg aaagccttgg agcctatgac caaatcttgc taaaaggttc
    19021 taactctatg aatctagcca aattggtaga acgtttagaa aatgaagcca agtgattttg
    19081 tcaagtatct gcaaagaatg attgccctta cagatactgg cttaaccttt acaaaagatc
    19141 cttttgaccg tgagcgctac gaggacttgc gaagtctgtt atctgaaatg ttgaatcagg
    19201 gatcagacct ggatgcagaa gaagtagcag aagtcttgaa accaacttca gcttatgcga
    19261 ctcccttaat ggacgtccgt gcttggattg ttgaggatga gaagatttgt ctggttaggg
    19321 gacaaggaga ggatagttgg gctttgccag gtggctttgg tgaagtcggc tattctccaa
    19381 ctgaaaatat tctcaaggaa attgaagaag aaaccggttt taaagccaaa gttgaaagac
    19441 tgctagctgt ttttgatacc aatcgtttcc aactacagag taaacaatat gcaaagtttg
    19501 tttttgaatg taagtttctc gatggacaat tccaagaaaa tcaagaaatt gcagaccttc
    19561 aattttttgc cattgatcaa ctgccagcct tatctgaaaa acgcattacc aaggagcaaa
    19621 tcgagattct ttggcaggtt tatcaaggtc agagggagca atatcttgac taagaagatg
    19681 attatcgtat ttttaaatcc atttttaaca actagcatgg tataataata tgcaggaaaa
    19741 ttttgaatta tgaggaagac tagatgaatt tatgggatat tttctttacg actcaggcaa
    19801 ccgagccgcc caaatttgac cttttttggt atgttagcct atttacgctc ttagccttaa
    19861 ccttttatac agcccatcgc tatcgtgaaa agaaggttta ccaacgattt ttccaaatct
    19921 tgcagactgt tcagttaatc cttctttatg gttggtactg ggtcaatcat atgccactgt
    19981 cagaaagcct acccttttac cattgccgta tggctatgtt tgtggtactc ttgcttcctg
    20041 gtcagtccaa atataaacaa tactttgcat tattgggaac atttgggaca ttagcagcct
    20101 ttgtttatcc agtgccagat gcttaccctt ttccacatat caccattcta tcctttatct
    20161 ttggtcattt agcactcttg gggaactctc tagtttatct attgagacag tataatgcgc
    20221 gattgctgga tgtgaaggga atttttctca tgacctttgc cctaaatgcc ttgatttttg
    20281 tggtcaattt ggtgacaggt ggcgattacg gatttttgac aaaaccgcca ttggttgggg
    20341 atcacggtct agtagctaat tatttacttg tttcaattgt gctggtagct actatcagtt
    20401 tgactaagaa aatcttagaa ttctttttag ctcaagaagc agaaaaaatg attgcaaagg
    20461 aagcttaaca cagagctttc ttttttgctc ttagagagtt tttacaagca gcttataaaa
    20521 taagaatttc tgaatagaca aactcaaaaa atggctggga aatttaggaa aaaagcaagc
    20581 acgattaaat tttttgtgtt ataatatttt gtgaatagct atgcctatgt ttagctatgg
    20641 aataatacga agtgcgaaac ttggaagata gagaggaagc gatgtaatgg ctagagaagg
    20701 cttttttaca ggtctagata ttggaacaag ctctgtcaag gtgcttgtgg ccgagcagag
    20761 aaatggtgaa ttaaatgtaa ttggcgtgag taatgccaaa agtaaaggtg taaaggatgg
    20821 aattattgtt gatattgatg cagcagcaac tgctatcaag tcagccattt cccaagcgga
    20881 agaaaaggca ggcatttcga ttaaatcagt gaatgtcggc ttgcctggta atcttttgca
    20941 ggtagaacca actcagggga tgattccagt aacatctgat actaaggaaa ttacggatca
    21001 agatgttgaa aatgttgtca aatcagcttt gacaaagagt atgacacctg accgtgaagt
    21061 cattaccttt attcctgaag aatttattgt ggatggtttc caagggattc gtgacccacg
    21121 tggtatgatg ggggttcgcc ttgaaatgcg tggtttgctt tatacaggac ctcgtactat
    21181 cttgcacaat ttgcgtaaga cggttgagcg tgcaggtgtt caggttgaaa atgttatcat
    21241 ttcaccacta gcaatggttc agtctgtttt gaacgaaggg gaacgtgaat ttggtgctac
    21301 agtgattgat atgggggcag gtcaaacgac tgtcgctaca atccgtaatc aagaactcca
    21361 gttcacacat attctccaag aaggtggaga ttatgtaact aaagatatct ccaaggtttt
    21421 gaaaacctct cgcaaattag cggaaggctt gaaactgaat tacggggaag cctatccgcc
    21481 tcttgcaagc aaagaaacct tccaagtaga ggttattgga gaagtggaag cagtcgaagt
    21541 gacggaagcc tacttgtcag aaattatttc tgcacgaatc aagcacatcc ttgaacaaat
    21601 caagcaagaa ttagatagaa ggcgtctatt ggacctccct ggtggtattg tcttaatcgg
    21661 tgggaatgcc attttaccag gtatggttga gcttgctcag gaagtctttg gcgtccgtgt
    21721 caagctttat gttccaaatc aagttggtat ccgtaatcca gcctttgcgc atgtgattag
    21781 tttatcagaa tttgcgggtc aattaacaga agttaatctt ttggctcagg gagcgataaa
    21841 aggtgagaat gacttaagtc atcagccaat tagttttggt gggatgctgc aaaaaacagc
    21901 tcagtttgta caatcaacgc ctgttcaacc agctcctgct ccagaagtag agccggtggc
    21961 gcctacagaa ccaatggcgg atttccaaca agcttcacaa aataaaccga aattagcaga
    22021 tcgtttccgt ggcttgatcg gaagcatgtt tgacgaataa agaggaaaaa taaattatga
    22081 cattttcatt tgatacagct gctgctcaag gggcagtgat taaagtaatt ggtgtcggtg
    22141 gaggtggtgg caatgccatc aaccgtatgg tcgacgaagg tgttacaggc gtagaattta
    22201 tcgcagcaaa cacagatgta caagcattga gtagtacaaa agctgagact gttattcagt
    22261 tgggacctaa attgactcgt ggtttgggtg caggaggtca acctgaggtt ggtcgtaaag
    22321 ccgctgaaga aagcgaagaa acactgacgg aagctattag tggtgccgat atggtcttca
    22381 tcactgctgg tatgggagga ggctctggaa ctggagctgc tcctgttatt gctcgtatcg
    22441 ccaaagattt aggtgcgctt acagttggtg ttgtaacacg tccctttggt tttgaaggaa
    22501 gtaagcgtgg acaatttgct gtagaaggaa tcaatcaact tcgtgagcat gtagacactc
    22561 tattgattat ctcaaacaac aatttgcttg aaattgttga taagaaaaca ccgcttttgg
    22621 aggctcttag cgaagcggat aacgttcttc gtcaaggtgt tcaagggatt accgatttga
    22681 ttaccaatcc aggattgatt aaccttgact ttgccgatgt gaaaacggta atggcaaaca
    22741 aagggaatgc tcttatgggt attggtatcg gtagtggaga agaacgtgtg gtagaagcgg
    22801 cacgtaaggc aatctattca ccacttcttg aaacaactat tgacggtgct gaggatgtta
    22861 tcgtcaacgt tactggtggt cttgacttaa ccttgattga ggcagaagag gcttcacaaa
    22921 ttgtgaacca ggcagcaggt caaggagtga acatctggct cggtacttca attgatgaaa
    22981 gtatgcgtga tgaaattcgt gtaacagttg tcgcaacggg tgttcgtcaa gaccgcgtag
    23041 aaaaggttgt ggctccacaa gctagaactg ctactaacta ccgtgagaca gtgaaaccag
    23101 ctcattcaca tggctttgat cgtcattttg atatggcaga aacagttgaa ttgccaaaac
    23161 aaaatccacg tcgtttggaa ccaactcagg catctgcttt tggtgattgg gatcttcgcc
    23221 gtgaatcgat tgttcgtaca acagattcag tcgtttctcc agtcgagcgc tttgaagccc
    23281 caatttcaca agatgaagat gaattggata cacctccatt tttcaaaaat cgttaagtaa
    23341 atgaatgtaa aagaaaatac agaacttgtt tttcgagaag ttgcagaggc tagtctgagt
    23401 gctcatcgag agagtggttc ggtctctgtc attgcagtta ccaagtatgt agatgtaccg
    23461 acagcggaag ccttgcttcc gctaggtgtt catcatatcg gtgaaaatcg tgtagataag
    23521 tttctggaaa aatatgaagc tttaaaagat cgagatgtga cttggcattt gattggtacc
    23581 ttgcaaagac gtaaggtgaa agatatcatt caatacgttg attatttcca tgcattggac
    23641 tcagtaaagc tagcagggga aattcaaaaa agaagtgacc gagtcatcaa gtgtttcctt
    23701 caagtaaata tttctaaaga agaaagcaaa cacggttttt cgagagagga actgctggaa
    23761 atcttgccag agttagccag actagataag attgaatatg ttggtttaat gacgatggca
    23821 ccttttgagg ctagcagtga gcagttgaaa gagattttca aggcgaccca agatttacaa
    23881 agagaaattc aagagaaaca aattccaaat atgcctatga ccgagttaag tatgggaatg
    23941 agtcgtgatt ataaagaagc gattcaattc ggttccactt ttgttcgtat aggtacatca
    24001 ttttttaagt aggagagaac catgtcttta aaagatagat tcgatagatt tatagattat
    24061 tttacggagg atgaggattc aagtctccct tatgaaaaaa gagatgagcc tgtgtttact
    24121 ccagtaaatt cttcacagga accggctctc ccaatgaatc aaccttcaca gtcggctggt
    24181 acaaaagaga acaatatcac cagacttcat gcaagacaac aggaattggc aaatcagagt
    24241 cagcgtgcaa cggataaggt cattatagat gttcgttatc ctagaaaata tgaggatgca
    24301 acagaaattg ttgatttatt ggcaggaaac gaaagtatct tgattgattt tcagtatatg
    24361 acagaggtgc aggctcgtcg ttgtttggac tatttggatg gagcttgtca tgttttagct
    24421 ggaaatttga aaaaggtagc ttctaccatg tatttgttga caccagtgaa tgttattgta
    24481 aatgttgaag atatccgttt accagatgaa gatcaacagg gtgagttcgg ttttgatatg
    24541 aagcgaaata gagtacgata atgatttttt taattcgtat gatttataat gcagtggata
    24601 tttactccct gattttggta gccttcgctg tcatgtcttg gtttccaggt gcctacgaat
    24661 ccagtttagg tcgttggatt gtagcgttgg tgaaaccagt gcttgctccc ttgcaacgcc
    24721 tgcctttaca gatagcgggt cttgatttat ctgtttgggt cgcgattgtt ttggttcgat
    24781 ttttaggaga aaacctagtg cgttttctgg cgatgatagg atgaataaag ggatttatca
    24841 gcatttctcc atagaagatc gtccatttct tgacaaggga atggaatgga taaagaaggt
    24901 agaagatagc tatgctcctt ttttaactcc ttttatcaat cctcatcagg agaagctatt
    24961 aaagattttg gccaaaacct atggtcttgc ttgtagcagt agtggggaat tcgtctcgag
    25021 tgagtatgtt cgagttttat tatacccaga ttatttccaa ccagagtttt cagattttga
    25081 aatatctctc caggaaattg tgtattccaa taaatttgaa catttaacgc atgctaagat
    25141 tttagggaca gtcatcaatc aattagggat tgaacggaaa ctttttggag atatcctagt
    25201 agatgaagaa cgggcgcaga ttatgattaa tcagcagttt cttcttctct ttcaagatgg
    25261 actaaagaaa attggtcgta tacctgtttc gctggaggaa cgtcctttca ccgagaaaat
    25321 agataagcta gaacagtatc gagaactgga tttatctgtg tctagttttc gattagatgt
    25381 tcttttatca aatgttttga aactatctag gaatcaagca aaccagttga ttgaaaagaa
    25441 acttgtccaa gtaaattatc atgtggtaga caaatcagat tacactgttc aagttggaga
    25501 cttgattagt gtgagaaaat ttggtcgctt gagattactt caagataagg gacaaacgaa
    25561 aaaagagaag aaaaaaataa ccgtccagtt attattaagt aagtgaggaa tagaatgcca
    25621 attacatcat tagaaataaa ggacaagact tttggaactc gattcagagg ttttgatcca
    25681 gaagaagtcg atgaattttt agatattgtg gttcgtgatt acgaagatct tgtgcgtgcg
    25741 aatcatgata aaaatttgcg tattaagagt ttagaagagc gtttgtctta ctttgatgaa
    25801 ataaaagatt cattgagcca gtctgtattg attgctcagg atacagctga gagagtgaaa
    25861 caggcggcgc atgaacgttc aaacaatatc attcatcaag cagagcaaga tgcgcaacgc
    25921 ttgttggaag aagctaaata taaggcaaac gagattcttc gtcaagcaac tgataatgct
    25981 aagaaagtcg ctgttgaaac agaagaattg aagaacaaga gccgtgtctt ccaccaacgt
    26041 ctcaaatcta caattgagag tcagttggct attgttgaat cttcagattg ggaagatatt
    26101 ctccgtccaa cagctactta tcttcaaacc agtgatgaag cctttaaaga agtggttagc
    26161 gaagtacttg gagaaccgat tccagctcca attgaagaag aaccaattga tatgacacgt
    26221 cagttctctc aagcagaaat ggaagaatta caagctcgta ttgaggtagc cgataaagaa
    26281 ttgtctgaat ttgaagctca gattaaacag gaagtggaaa ctccaactcc tgtagtgagt
    26341 cctcaagttg aagaagagcc tctgctcatc cagttggccc aatgtatgaa gaaccagaag
    26401 tagctccagt gcatccgaca ggtccaacac cagctacaga aactgttgat tcagcaccag
    26461 gatttgaagc accgcaagaa tccgttacaa ttttataaga aacgatgtga gaacaatatc
    26521 ttatccttat atttccagcg agcaggagat ggtgtgagtc ctgcattccc taacgataag
    26581 attatcctct caaaaactca agtctgaatc tagtaagatt tgacgtttcc cacgttacgg
    26641 gataagaggg agaaagacta aatctttttc cgaataaagg tggtaccacg attttcgtcc
    26701 tttttggaag tcgtggtttt taatttgtta ttatttataa aggagatacc atgaaactaa
    26761 aagataccct taatcttggg aaaacagctt tcccaatgcg tgcaggcctt ccgaccaaag
    26821 agccagtttg gcaaaaggaa tgggaagatg caaaacttta ccaacgtcgt caagaactaa
    26881 atgaagggaa accgcatttt gtcttgcatg atggccctcc atacgcaaac ggaaatatcc
    26941 acgttggaca tgccatgaat catatctcaa aagatatcat cattcgttct aagtctatgt
    27001 caggttttaa cgcaccttat atcccaggtt gggatactca tggtctgcca atcgagcaag
    27061 ttttggcaaa gcaaggtgtg aagcgcaaag aaatggactt ggttgagtac ttgaaacttt
    27121 gccgtgaata cgctctttct caagtttata aacaacgtga tgatttcaag cgcttgggga
    27181 tgtcaggtga ctgggaaaat ttatatgtga ctttaactcc agactacgaa gcagcccaaa
    27241 tccgtgtatt tggtgagatg gctaataagg gttatatcta ccgtggtgct aagccagttt
    27301 actggtcttg gtcatctgag tcagcccttg ccgaagcgga aattgaatac catgatttgg
    27361 tttcaacttc cctttactat gccaataagg taaaagatgg caaaggtgtt ctagatacag
    27421 atacttatat cgttgtttgg acaactactc catttactat cacagcttct cgtggtttga
    27481 ctgttggtgc agatattgat tacgttttgg ttcaacctgc tggtgaaact cgtaagtttg
    27541 tcgttgctgc agaattgttg actagcttgt ctgagaaatt tggctgggtt gatgttcaag
    27601 ttttggcaac ttaccgtggt caagaactca accacatcgt aacagaacat ccatgggata
    27661 cagctgtaga tgaattggtt atccttggtg accacgttac aactgactct ggtacaggta
    27721 ttgtccatac agcccctggt tttggtgagg acgattacaa tgttggtatt gctaataatc
    27781 ttgaagtcgc agtgactgtt gatgaacgtg gtatcatgat gaagaatgct ggtcctgaat
    27841 ttgaaggtca attctatgaa aaggtagttc caactgttat tgaaaaactt ggtaacctcc
    27901 ttcttgccca agaagaaatc tctcactcat atccatttga ctggcgtacc aagaaaccaa
    27961 ttatctggcg tgcagtacca cagtggttcg cctctgtttc aaaattccgc caagaaatct
    28021 tggatgctat tgataaagtt aaattccata cagaatgggg taaagtacgt ctatataata
    28081 tgattcgtga ccgtggtgac tgggttatct ctcgtcaacg tgcttggggt gttccacttc
    28141 caatcttcta tgcagaagac ggtacagcta tcatgacagc tgagactatc gaatatgtgg
    28201 ctcaactctt tgaagtacat ggttcaagca tttggtggga acgtgatgcc aaagacctct
    28261 tgccagaagg atttacacat ccaggttctc caaatggcga gttcaaaaaa gaaactgata
    28321 tcatggacgt ttggtttgac tcaggttcat catggaatgg tgttttggta aatcgtccaa
    28381 atctgactta tccagctgac ctttaccttg aaggttctga ccaataccgt ggttggttca
    28441 actcatcact cattacatct gttgccaacc atggcgtagc accttacaaa caaatcttgt
    28501 cacaaggttt tacccttgac ggtaaaggtg agaagatgtc taaatctctt ggaaatacca
    28561 ttgctccaag cgatgttgaa aaacaatttg gtgctgaaat cttgcgtctc tgggtaacaa
    28621 gtgttgactc aagcaatgac gtgcgtatct ctatggatat cttgagccaa gtctctgaaa
    28681 cttaccgtaa gattcgtaat actcttcgtt tcttgattgc caatacatct gactttaacc
    28741 caactcaaga tgcagtcgct tacgatgaac ttcgttcagt tgataagtac atgaccattc
    28801 gctttaacca gcttgtcaag accattcgtg atgcctatgc aaactttgaa ttcttgacaa
    28861 tctacaaggc cttggtgaac tttatcaacg ttgatttatc agccttttac cttgattttg
    28921 ctaaagatgt tgtttacatc gaaggtgcta aatcactgga acgtcgtcaa atgcagactg
    28981 tcttctatga tattcttgtc aaaatcacca aactcttgac accaatcctt cctcacactg
    29041 cggaagaaat ctggtcatat cttgagtttg aaacagaaga cttcgtccaa ttgtcagaat
    29101 taccagaagt tcaaactttt gctaaccaag aagaaatctt ggatacatgg gcagccttca
    29161 tggactttcg tggacaagca caaaaagcct tggaagaagc tcgtaatgca aaagttatcg
    29221 gtaaatcact tgaagcacac ttgacagttt atccaaatga agtggtgaaa actctactcg
    29281 aagcagtaaa tagcaatgtg gctcaacttt tgatcgtgtc tgagttgacc atcgcagaag
    29341 gaccagctcc ggaagctgcc cttagcttcg aagatgtagc cttcacagtt gaacgtgctg
    29401 ctggtgaagt atgtgaccgt tgccgtcgta ttgacccaac aacagcagaa cgtagctacc
    29461 aggcagttat ctgtgaccac tgtgcaagca tcgtagaaga aaactttgcg gaagcagtcg
    29521 cagaaggatt tgaagagaaa taagattgaa aagtctaggc aaaattcaat ttgagaagaa
    29581 aagacaacta attttatagt ctattaaacg cattgtatca cgtttttgaa cacctgatat
    29641 gatgcgtttt ttatttattt taaaaatttg cgaggtatga ctttttatac tcaacaagaa
    29701 tcaaagagaa acttagcaag ctaacagtag taagataaaa tagga
//
LOCUS       DAVLYR010000008        29420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLYR010000008 DAVLYR010000000
VERSION     DAVLYR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.008"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(34..393)
                     /locus_tag="VYO63_000352"
     CDS             complement(34..393)
                     /locus_tag="VYO63_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1671334.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            416..994
                     /locus_tag="VYO63_000353"
     CDS             416..994
                     /locus_tag="VYO63_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846832.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEV1671335.1"
                     /translation="METRNRGIYRKVRLLRLWVIFVIMKVMKSYNTLNDYYRKLFGEK
                     TFKVPIDAGFDCPNRDGTVAHGGCTFCTVSGSGDAIVAPDAPIREQFYKEIDFMHRKW
                     PDVQKYLVYFQNFTNTHEKVEVVRERYEQAINEPGVVGINIGTRPDCLPDETIEYLAE
                     LSECMHVTVELGLQTTYEATSDLINRAHSYEL"
     gene            995..1441
                     /locus_tag="VYO63_000354"
     CDS             995..1441
                     /locus_tag="VYO63_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1671336.1"
                     /translation="METVKRLRKYPKIEIVSHLINGLPGETHEMMVENVRRCVTNNDI
                     QGIKLHLLHLMTNTRMQRDYHEGRLQLMSQDEYVRVICDQLEIIPKHIVIHRITGDAP
                     RDMLLGLMWSLNKWEALNSIEMEMRRRGSVQGCKAVKQEFENEKTT"
     gene            1425..1982
                     /locus_tag="VYO63_000355"
     CDS             1425..1982
                     /locus_tag="VYO63_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1671337.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            1975..2229
                     /locus_tag="VYO63_000356"
     CDS             1975..2229
                     /locus_tag="VYO63_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671338.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            2241..4295
                     /locus_tag="VYO63_000357"
     CDS             2241..4295
                     /locus_tag="VYO63_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1671339.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            4474..5334
                     /locus_tag="VYO63_000358"
     CDS             4474..5334
                     /locus_tag="VYO63_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1671340.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILIMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            5545..6255
                     /gene="ccdA2"
                     /locus_tag="VYO63_000359"
     CDS             5545..6255
                     /gene="ccdA2"
                     /locus_tag="VYO63_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1671341.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            6275..6841
                     /locus_tag="VYO63_000360"
     CDS             6275..6841
                     /locus_tag="VYO63_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1671342.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            6852..7964
                     /gene="msrB"
                     /locus_tag="VYO63_000361"
     CDS             6852..7964
                     /gene="msrB"
                     /locus_tag="VYO63_000361"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1671343.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            8322..9059
                     /locus_tag="VYO63_000362"
     CDS             8322..9059
                     /locus_tag="VYO63_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1671344.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            9056..10747
                     /locus_tag="VYO63_000363"
     CDS             9056..10747
                     /locus_tag="VYO63_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1671345.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            10863..11426
                     /locus_tag="VYO63_000364"
     CDS             10863..11426
                     /locus_tag="VYO63_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1671346.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            11448..17390
                     /locus_tag="VYO63_000365"
     CDS             11448..17390
                     /locus_tag="VYO63_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1671347.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEDTQPEVVETKDEAANQP
                     VEEPKVETPAVEKQTEPTEEPKVEQVGEPVEPREDEKAPVSPEKQPEAPEEEKTAEET
                     PKQEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLETAKGVYASE
                     PVKQPEVNSETKAEKVAANTDAKQSEVNSETASLKTAISGLNTDKVELENQLKIAQGK
                     TETDFSMESWTVLSTAKNKAQEVKDNGTATQEQINEAEKSLKTALADLSVDKTALGSA
                     IDTATKKNKENYTNQTWAELETVLTAAKSVNTNESKQSEVNEAVEKLTATIEKLVELS
                     EKPRLTLSIEKRDIDRKVTVTYTLENPANTQIKSITATLKKGEEVVKDFVLTEENLKT
                     NHLTALFEKLDYYKEYTLSTDMVYNRGNDDETESISEELIQLNLKKLELKDIQTVSLM
                     KFENGQESQVTHLSDKPTDLSKLYLKVTSSTSKDAVLAVSSIEEEIVENKKIFKIHAD
                     TPELVVRKKDGSLSKGFDYYMERVIPHDGDIYYDFKDLISAMTSNPTGTFILGRDISS
                     RNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIFDLEYPLFDTIKSGVVKDIDFKHVNM
                     VFPDSNQGDNVATIARVIKDKTKIENVNVEGYLEGRDHVAGLVNNLEGNSEIENISFT
                     GKIKSKGGNSITAGIAGRNILSRVKRAYVNANIEVLGSTNSSMLVAVNGTTLNASGGW
                     GAWGRLTESVAKGTLEIKRSGQAGGVTATVWPYGAIDKVVSYAKVTKGKELFGSDGDL
                     NNNWFMQKINNIFGVQGISSGDSGNDSKFKRISEEEAKQKVASYNITAPNLMSDSSLL
                     VDRLNESWKNTDQFESIQDYQSQNQLIYQNLTKFTPYYNKEFIVHEGNALTPEQEILK
                     TKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGSQKRYKVTSTGKFSITNLPEYQVEDL
                     NVVYTSEHIVHPLDSSLINNLVEELKKVELYTESTYQVLGIDKDNANKLNRTKRLFLD
                     ESLDAVKTQLPTFVKTMFENEWLHINGESSGAVAALRQKIMDNKTAILLALTYINRYY
                     DVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRLGSSGENRLKGSENAETFKQLFASET
                     KQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDYIQFEERPSLVEEIKDAKYRVYDNLT
                     APYYQGYILPLLTLKNTHLAILSNYSTMTFVSREKRPNWKNEDFDKWVKYVATAHRNH
                     VDTWYKILPDNIKGKMVKENVTAVWEGLSIPGSEWVDQNAVDRKGRDYAPAREFFNLV
                     GGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSEYGISVFTHELTHVNDTWIYLGGYGR
                     RENMGPEAYAQGLFQSPVPGQPGWGALGLNMAFERKNDGDLIYNASPTQFENRKELDS
                     YMKNYNDTLMMVDYLEGDAVISKGKEAITKWFKKVEPKVVSQTAQYDTVRQLTAEEKE
                     KLSVSSVDDLVDQGLMSDRAVGNNTYNPADFETSYIAIDYMTGIYGGGKNSVGSPGAL
                     MFKHNTFRMWGYYGFEEGVLGYASNKFKQASRDEGHAGLSDNFIISKISKGEFLTMEA
                     FKKGYFKKVVEELKTKGIRPVTINQKTYSTFEELQEGFKQAVERDLKKNQLDERETRN
                     FKFQVFRQLLQQTDSFKTSIFR"
     gene            17451..19172
                     /gene="pabB"
                     /locus_tag="VYO63_000366"
     CDS             17451..19172
                     /gene="pabB"
                     /locus_tag="VYO63_000366"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1671348.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            19173..19790
                     /locus_tag="VYO63_000367"
     CDS             19173..19790
                     /locus_tag="VYO63_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1671349.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SFMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            19840..20718
                     /locus_tag="VYO63_000368"
     CDS             19840..20718
                     /locus_tag="VYO63_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1671350.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEREPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYARNAWQGN
                     YYLKSDGKMAKGEWVYDATYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGG
                     RYYVGADGVWKEGQASTVSSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFD
                     KFSNDAAQYAIDHLDD"
     gene            20845..21804
                     /locus_tag="VYO63_000369"
     CDS             20845..21804
                     /locus_tag="VYO63_000369"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1671351.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            21901..22740
                     /locus_tag="VYO63_000370"
     CDS             21901..22740
                     /locus_tag="VYO63_000370"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1671352.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(22788..22958)
                     /locus_tag="VYO63_000371"
     CDS             complement(22788..22958)
                     /locus_tag="VYO63_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1671353.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            23079..23963
                     /gene="miaA"
                     /locus_tag="VYO63_000372"
     CDS             23079..23963
                     /gene="miaA"
                     /locus_tag="VYO63_000372"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1671354.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            23956..25194
                     /gene="hflX"
                     /locus_tag="VYO63_000373"
     CDS             23956..25194
                     /gene="hflX"
                     /locus_tag="VYO63_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1671355.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            25187..25810
                     /locus_tag="VYO63_000374"
     CDS             25187..25810
                     /locus_tag="VYO63_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1671356.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            25825..26754
                     /locus_tag="VYO63_000375"
     CDS             25825..26754
                     /locus_tag="VYO63_000375"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1671357.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            26775..27530
                     /locus_tag="VYO63_000376"
     CDS             26775..27530
                     /locus_tag="VYO63_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1671358.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(27584..28492)
                     /locus_tag="VYO63_000377"
     CDS             complement(27584..28492)
                     /locus_tag="VYO63_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1671359.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            28556..28825
                     /locus_tag="VYO63_000378"
     CDS             28556..28825
                     /locus_tag="VYO63_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1671360.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            28825..29205
                     /locus_tag="VYO63_000379"
     CDS             28825..29205
                     /locus_tag="VYO63_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1671361.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         8924 a         5364 c         6657 g         8475 t
ORIGIN      
        1 gcaaaaatcc ctagaaaatg cttagggatt cgactatttc attaagaaca attcaacaat
       61 catttctata tacatgataa gggttaataa gaatcctgca cgccagaaga atttgataaa
      121 tttaggatag taaaagctgc gttttttcaa aaggaagaaa aagacgagaa taatggctag
      181 gatcgagagg gctagcccca gtctagggag gaaattatgg gtaaaggttt tagctgtaat
      241 caagtaatac tcaaatacca agactggaaa agccaaatcc gcaaagtttc gtcctagttt
      301 ttttaatcta aaaagtttgg ttaggataat gcagacgact aaggtcagta tcaataataa
      361 aatagatgct aatttcatta aaatcatacc catattgtat cataaaaaaa ggctaatgga
      421 aacgagaaac agaggaattt ataggaaagt caggcttttg agattgtggg tgatttttgt
      481 tataatgaaa gtcatgaaat cttataatac cttgaatgat tattatcgaa aactctttgg
      541 agaaaagact tttaaagtcc ctattgatgc gggatttgac tgtcccaatc gtgatggaac
      601 tgtggctcat ggaggctgta ctttttgtac ggtttcaggt tctggagatg ctattgtagc
      661 cccagatgcg cctatccgtg agcaatttta taaggaaatt gactttatgc accgtaagtg
      721 gccggatgtt cagaagtatc tggtttattt tcaaaatttt accaacaccc atgaaaaggt
      781 ggaagttgtt cgagagcgtt atgaacaggc tatcaatgag ccaggtgtgg taggtatcaa
      841 tattggaacg cgcccagact gtttaccaga cgaaaccatc gaatatttgg ctgagttatc
      901 ggagtgcatg catgtgacgg ttgaattggg cttgcaaact acttatgaag caacctctga
      961 cctgattaac cgtgcgcatt cttatgaatt gtaggtggaa acggtcaagc gtttgagaaa
     1021 gtatccaaaa attgagattg tttcccattt gatcaatggt ttgcctggtg aaacccatga
     1081 gatgatggtt gaaaatgtcc gccgttgtgt cacgaataac gatattcaag ggattaaact
     1141 gcatttgctc catcttatga caaatactcg tatgcaacga gattaccatg agggacgttt
     1201 acaattgatg agtcaggacg aatatgtcag ggtcatctgt gaccaactgg aaatcattcc
     1261 caaacatatc gtcatccatc gaattacagg agatgcgcct agggatatgc tgcttgggct
     1321 tatgtggagc ctcaataaat gggaagctct caacagcatt gagatggaga tgcgacgtcg
     1381 tggaagtgtt caaggatgca aggctgtaaa acaggagttt gaaaatgaaa agaccacttg
     1441 agatggcaca tgattttttg gctgaggtcg tgacaaaaga ggatgtcgta gtggatgcga
     1501 ctatgggaaa tggtcatgac acgctttttt tagccaagct agccaagcaa gtctatgcct
     1561 ttgatattca gaagcaagcc ttggaaaaga cccaagagcg tttgcatcag gctggcttga
     1621 caaatgccca gttaatcttg caaggccatg agacactgga ccagtttgtg acaaaagcta
     1681 aggcagggat ttttaatttg ggctatttgc cgtcagctga taagtctgtc atcacccgac
     1741 cgcagacaac gattgaggca ttagaaaagc tatgtggctt acttgtcaaa ggtggacgaa
     1801 ttgctattat gatttactat ggtcatgaag gaggcgacct cgagagagat gctgtcttgg
     1861 attttgtgat ccagttgaac caacaagagt acacagctgc catttaccga actttaaacc
     1921 aagtcaacaa cccgccgttt ttagtgatga ttgaaaaatt agagagatac agacatggat
     1981 aaacaatacc tacacgaaaa gctggatgcc atgcgccaga attttgttga atcaacccac
     2041 cacgaacgag cggtgggcgt gctagaccaa gcgcatatga gcaaaaaaat gcttaaaatc
     2101 aagaaaaaat tagttgctct tgaaatggaa cggtgccaga gaaaaattga gcacaaagac
     2161 tgttccaaga ttgaccaaaa aatcaaagag cagaaggaga tatttgaatc ctgttgtaaa
     2221 aaagattaag gaggacgtgc gtggaattac tgatttacct catcctattt ttactggttt
     2281 tgattgtctc aagtacaacc aataagctcc tgcccttttt gcctctccct ttggtgcaga
     2341 ttcttttggg aattgtgatt ggtctctttt tacccaatac tgactttcac cttaatacgg
     2401 agttgttttt ggcactggtt atcggaccct tgcttttccg agaggctgaa gaagcagatg
     2461 ttacggctat tttaaaacac tggcgaatca ttgtttatct catatttcca gtgattttta
     2521 tctcgaccct gagtttgggt ggcttggccc atcttctttg gttcagcctt cccttggcag
     2581 cttgcttggc tgttggtgca gcccttggtc ctacggactt ggtggccttt gcctctcttt
     2641 cggagcgttt tagctttcct aagcgcgtgt ccaatattct taagggcgaa ggactcttga
     2701 atgatgcttc tggtttggtg gcttttcagg tagctttgac agcttggaca actggagctt
     2761 tttctctggg gcaagctagc agttcgctca tcttttcaat cctaggcggt tttttaattg
     2821 gatttttaac agccatgacc aaccgcttcc tccatacctt cttgctaagt gtgcgcgcaa
     2881 cggatattgc cagtgaactt ttattagaat tgagtttgcc tctagtgacc ttctttctgg
     2941 cagaagaagt ccatgtttca gggattattg ccgtcgtagt tgctggaatt ttaaaggcaa
     3001 gtcgcttcaa gaaaatcacg ctcctcgaag cccaagtgga tacggtgacc gagacggtct
     3061 ggcatacagt gacctttatg ctcaacggtt ctgtctttgt gattttaggg atggagttgg
     3121 aaatgatagc agaacctatc ttgaccaatc caatctataa tcctctactt ttattgctat
     3181 ctctcatcgc ccttaccttt gtcctctttg tcattcgttt tattatgatc tatggctatt
     3241 atgcctatag aacccgacgc ctcaagaaaa agctaaataa gtatatgaag gacatgtttc
     3301 tcttgacctt ttcaggtgtt aagggaacgg tgtcgattgc tacgattctc ttgataccaa
     3361 gtaatctaga acaggagtat cctctcttgc ttttccttgt tgcaggtgtg acgcttgtca
     3421 gctttttaac aggtctcttg gtcttgcctc atctttctga tgaagaggaa gaaagcaagg
     3481 attatctcat gcatatcgcc attttgaatg aagtaacgct agagttggaa aaagagttgg
     3541 aagacaccag aaataaactt cccctctatg cggctattga caattatcat ggacgtattg
     3601 aaaatctcat tttaagccaa gaaaaccagg atgatcaaga agattgggct gctttgaaac
     3661 tcttgattct tagtattgaa agtgatggtt tggagcaggc ctatgaagag gggaacatta
     3721 gcaatcgtgc ttaccgagtt taccaacgtt atctgaaaaa tatagaacaa ggaatcaatc
     3781 gtaaacttgc ctcaagactg acctattatt tccttgtttc tttgcggatt ttacgttttc
     3841 tccttcatga agtttttact ctcggaaaga ccttccgtag ctggaagaac aaggagcaaa
     3901 gccgtctccg tgctcttgat tatgaccaaa ttgcagagct ctatcttgcc aatacagaga
     3961 tgattattga aagtttggaa aacctgaagg gagtctacag acgctctttg attagtttta
     4021 tgcaggagtc tcgtcttcga gaaacagcta ttatcagcag tggtgccttt gtcgaacggg
     4081 ttatcaatcg tgtcaaaccc aacaatatcg atgaaatgct gagaggctat tatctggagc
     4141 gcaagttgat tttcgaatac gaagaaaaac gattgattac gactaagtat gccaagaaat
     4201 tacgacaaaa tgtaaataac ttagagaact attccttgaa ggaagctgcc aataccctac
     4261 cgtatgatat ggtggaattg gtaagaagaa attagttaat actcttcgaa aatctcttca
     4321 aaccacgtca gcgtcgcctt accgtatgta tgtgactgac ttcgtcagtc ttatctacaa
     4381 cctcaaaaca gtgttttgag cagcttgcgg ctagctttct agtttgctct ttgattttca
     4441 ttgagtataa gattataaat gaaggagtat gacatgaaaa agtggtggaa agagctgata
     4501 gacaaacctt tattaaaagc ttttttgcat tattatcaag catcagatag tgagttgacc
     4561 agtgtcgcag tagcctacta ttggttgatt tcgattttcc ccctgctttt ggtggtggtc
     4621 aatatcctcc cctattttca gattcctgtg ggagaatttt tgggctttat gaaagacgtc
     4681 ctgcccccaa gcctctatga aggtgtggaa aaaatagcca gagaagtctt gacgcaacct
     4741 tcaacaggtt tattgagttt ttctgtttta tcggcgttat ggagtttttc aaaatctatg
     4801 aatttcttgc aaaaagcttt taacaaggct tatggcgtcg aaaagagtcg tggacttatt
     4861 tcccatcaga tgctaagtct cctagtcagt tttggcttgc agctcctctt tgcgtttgcc
     4921 ttgtttttga tcttatttgg tcagatgatt ttggctttac ttgctcatta ttggacaaaa
     4981 gatggcatta tctatcaggc tttacaaggg ttggcaggtc ctctgattta cgccctcctc
     5041 tttgctatct tgatcatgct ttattatttt cttcccaatc tatctaacag gaaaattagc
     5101 tataccctac caggcagtgc ctttgttctc ttagtgattt taggtttact aactctgttc
     5161 tctagttatc tcaattacta tgtccatcat ttggtggatg tccgaatttt aggttccgtt
     5221 cttcttgttg ttatgatgtt ttggtttatc ttgattgcta aaattgttat cctaggcgcg
     5281 gtgatcaatg ccagtatgca gagtttgaaa gatccggtct ttaaaaaaga ctaactattt
     5341 atccattaca aaagcgcata gtatcaagat tctataaagc cttgatacta tgcgcttttg
     5401 tgctttgaca gttttttgta tagtttttgt ccaagatttc taaatcatct gattaaattc
     5461 ctattttccc ctatcattgt ccctgttttt tcgaaaatgg aaggagtata ataaatctat
     5521 caataaaatg ataggaggaa ctttatgggt catattttct tttttctaag tgtctttttg
     5581 gcagggattc tatccttctt ttctccttgt atcttacctt tgttaccggt ctatacagga
     5641 gtgttactag atgataagga tggtgctcag gcttctagcg gcaaattttc aatctcagtt
     5701 actagtttat tacgaactct ggcctttata gcaggaattt cctttatatt tattttgttg
     5761 ggctatggag ctggtttttt aggcgatttg ctttatgctt cttggttcca atatcttact
     5821 ggggcaatta ttatccttct tggtttgcac caaatggaga ttctacactt taaggggctt
     5881 tataaggaaa agaggctaca actgcaagga caggggcaaa atggtaaggg ctatagtcag
     5941 gcatttttat tgggcttgac ctttagtttt gcttggacgc cttgcgtggg gccggttctg
     6001 gggtctgttt tggccttggc ggcttcaggt ggttcaggag cttggcaggg agctggtctc
     6061 atgttggtgt atacgctggg cttggcgcta ccattcttgc ttctagctct gacctctagt
     6121 tatgttttga aacatttccg aaaacttcat ccctatctcg gaatcctcaa aaaagtgggt
     6181 ggttttctca ttattgtgat gggattcttg gttctgtttg gaaatgcttc aattttaagt
     6241 caattatttg aataaaatgg aaaggaatat caatatgaaa aaatggcaaa catgtgttct
     6301 tggagcaggt tcgctccttt gtttgacggc ttgttcaggc aagtccgtga ctagtgaaca
     6361 ccaaacgaaa gatgaaatga agacggagca gacagctagt aaaacaagcg cagctaaagg
     6421 gaaagaggtg gctgattttg aattgatggg agtagatggc aagacctacc gtttatctga
     6481 ttacaagggc aagaaagtct atctcaaatt ctgggcttct tggtgttcta tctgtctggc
     6541 tagtcttcca gatacggatg aaattgctaa agaagctggt gatgactatg tggtcttgac
     6601 agtagtgtca ccaggacata agggagagca atctgaagcg gactttaaga attggtataa
     6661 gggattggat tataaaaatc tcccagttct agttgaccca tcaggcaaac ttttggaaac
     6721 ttatggtgtc cgttcttacc caacccaagc ctttatagac aaagaaggca agctggtcaa
     6781 aacacatcca ggattcatgg aaaaagatgc aattttgcaa actttgaagg aattagccta
     6841 ggaggcgtct tatgaatgat aagttaaaaa tcttcttgtt gctaggagta ttttttctag
     6901 ccataaccgg tttctatgtt ctattgatac gaaatgcagg gcagacagat gcctcgcaaa
     6961 ttgaaaaggc ggcagttagc caaggaggaa aagcagtgaa aaaaacagaa attagtaaag
     7021 acgcagactt gcacgaaatt tatctagctg gaggttgttt ctggggagtg gaggaatatt
     7081 tctcacgcgt tcccggggtg acggatgccg tttcaggcta tgcaaatggt agaggagaaa
     7141 caaccaagta cgaattgatt aaccaaacag gtcatgcaga aaccgtccat gtcacctatg
     7201 atgccaagca aatttctctc aaggaaatcc tgcttcacta tttccgcatt atcaatccaa
     7261 ccagcaaaaa taaacaagga aatgatgtgg ggacccagta ccgtactggt gtttattaca
     7321 cagatgacaa ggatttggaa gtgattaacc aagtctttga tgaggtggct aagaaatacg
     7381 atcaacctct agcagttgaa aaggaaaact tgaagaattt tgtggtggct gaggattacc
     7441 atcaagacta tctcaagaaa aatccaaatg gctactgcca tatcaatgtt aatcaggcgg
     7501 cctatcctgt cattgatgcc agcaaatatc caaaaccaag tgatgaggaa ttgaaaaaga
     7561 ccctgtcacc tgaggagtat gcagttaccc aggaaaatca aacagaacga gctttctcaa
     7621 accgttactg ggataaattt gaatccggta tctatgtgga tatagcaact ggagaacctc
     7681 tcttttcatc aaaggacaag tttgagtctg gttgtggctg gcctagtttt acccaaccca
     7741 tcagtccaga tgttgtcacc tacaaggaag ataagtccta caatatgacg cgtatggaag
     7801 tgcggagccg agtaggagat tctcaccttg gccatgtctt tacggatggt ccacaggaca
     7861 agggcggctt acgttactgt atcaatagcc tctctatccg ctttattccc aaagaccaaa
     7921 tggaagaaaa aggctacgct tatttactag attatgttga ttaatactct tcgaaaatct
     7981 cttcaaacca cgtcagcgtc gccttaccgt atgtagggtt actgacttcg tcagttttat
     8041 ctacagcctc aaagctatac tttgagctga cttcgtcagt tttatctaca gcctcaaagc
     8101 tatactttga gctgacttcg tcagttttat ctacaacctc aaagctatac tttgagctga
     8161 cttcgtcagt tttatctaca acctcaaagc ggtactttga gaaacctgtg gctatcttcc
     8221 tagtttactt tttgattttt attgagtata agaggtcttt cctaagcagt tagaggagaa
     8281 ttttgctata ctgatactag taagtggtaa aggagcagat catgacctac acaatcttaa
     8341 tcgtagaaga tgaatatctg gtaagacaag gtttgactaa actggtcaat gtagcagcct
     8401 acgatatgga aatcatcggt caggctgaaa atggaaggca ggcttgggaa ttgatccaaa
     8461 agcaggtgcc agatatcatt ttaaccgata tcaacatgcc tcatctaaat ggcatccagt
     8521 tggccagtct ggtacgagaa acctatcctc aggttcattt ggtcttttta acaggttacg
     8581 atgattttga ttatgccttg tctgctgtca aactaggtgt ggacgactac ctgctcaaac
     8641 ccttttctcg tcaggatatt gaggaaatgt tggggaaaat caaacaaaaa ctagacaagg
     8701 aagagaaaga agagcagtta caagatttat tgactaatag gtttgaagga aacatggccc
     8761 agaaaatcca gtctcatctg gctgatagcc aatttagttt aaagtcttta gccagtgact
     8821 taggttttag tccgacctat ctgagttcct tgattaagaa agagttgggc ttgccttttc
     8881 aggattatct ggtgagagaa cgtgttaaac aagccaagct cttgctttta actacagatc
     8941 tgaagattta tgagatcgca gagaaggttg gttttgaaga tatgaactat tttacccaac
     9001 gttttaagca gattgcaggt gtgacacctc gtcagtttaa gaagggagaa gaccgatgaa
     9061 gcgttcttct cttttagtta gaatggttat ttccatcttt ctggtctttc tcattctcct
     9121 agctctggtt ggaactttct actatcaatc aagttcttca gccattgagg ccaccattga
     9181 gggcaacagc caaacgacca tcagccagac tagccacttt attcagtctt atatcaaaaa
     9241 actagaaacc acctcgactg gtttgaccca gcagacggat gttctggcct atgctgagaa
     9301 tcccagtcaa gacaaggtcg agggaatccg agatttgttt ttgaccatct tgaagtcaga
     9361 taaggacttg aaaactgttg tgctggtgac caaatctggt caggtcattt ctacagatga
     9421 cagtgtgcag atgaaaactt cctctgatat gatggctgag gattggtacc aaaaggctat
     9481 tcatcaggga gctatgcctg ttttgactcc agctcgtaaa tcagatagtc agtgggtcat
     9541 ttctgtcact caagaacttg ttgatgcaaa gggagccaat cttggtgtgc ttcgtttgga
     9601 tatttcttat gaaactctgg aagcctatct caatcaactc cagttggggc agcagggctt
     9661 tgccttcatt atcaatgaaa accatgaatt tgtctaccat cctcaacaca cagtttatag
     9721 ttcgtctagc aaaatggagg ctatgaaacc ctacatcgag acaggtcagg gttatactcc
     9781 tggtcacaaa tcctacgtca gtcaagagaa gattgcagga actgattgga cggtacttgg
     9841 cgtgtcatca ttggaaaagt tagaccaggt tcggagtcag ctcttgtgga ccttgcttgg
     9901 ggccagtgtc acatctcttc ttgtctgtct ctgcttagtg tggttcagtc ttaaacgctg
     9961 gattgctcct ttgaaggatt tgagagaaac catgttggaa attgcttctg gtgctcaaaa
    10021 tcttcgtgcc aaggaagttg gtgcctatga actgagagaa gtaactcgcc aatttaatgc
    10081 catgttggat cagattgatc agttgatggt agctattcgt agccaggaag aaacgacccg
    10141 tcagtaccaa cttcaagccc tttcgagcca gattaatcca catttcctct ataacacttt
    10201 ggacaccatc atctggatgg ctgaatttca tgatagtcag cgagtggtgc aggtgaccaa
    10261 gtccttggca acctatttcc gcttggcgct caatcaaggc aaggacttga tttgtctctc
    10321 tgacgaaatc aatcatgtcc gccagtatct ctttatccag aaacaacgct atggagataa
    10381 gctggaatac gaaattaatg aaaatgttgc ctttgataat ttagtcttac ccaagctggt
    10441 cctacaaccc cttgtagaaa atgctcttta ccatggcatt aaggaaaagg aaggtcaggg
    10501 ccatattaaa ctttctgtcc agaaacagga ttcgggattg gtcatccgta ttgaggatga
    10561 tggcgttggc ttccaagatg ctggtgatag tagtcaaagt caactcaaac gtgggggagt
    10621 tggtcttcaa aatgtcgatc aacggctcaa acttcatttt ggagccaatt accagatgaa
    10681 gattgattct agaccccaaa aagggacgaa agttgaaata tatataaata gaatagaaac
    10741 tagctaactc ccagtcaatt ctgggagttt tatgcgtaat tgggcaaact ttataggttg
    10801 tctatcttgc taaaaacaag aaaaaacggt atgatagaag agtgacaaca gaggtaacag
    10861 agatgaacga gacacagatt caaagagaaa cacgtcaggt agtggaagat gttttagaaa
    10921 agaccaattt gaagcagggg gtcctttttg ttttaggtct atcttctagt gaggtgctag
    10981 gtggtcagat tgggaaagaa tccagtcaag agattggtga gctgatagtg gagactatct
    11041 tgggtattct aggtagtaga ggtatccatt tagcagttca aggttgtgag catgtcaatc
    11101 gggctctcgt tgttgaacgt caggtggcag agcagtttga tctggaaatt gtcagtgtcc
    11161 atcctactct tcatgcagga ggttcggggc agttggcagc ctttaagttt atgcaggatc
    11221 cagttgaggt tgaatttatc aaggctcatg ctggattgga tatcggagac actgcaattg
    11281 gcatgcatgt caagcatgtt caggttccga ttcgccctat tttgagagag attggtcatg
    11341 cccatgtaac ggcactggct agtcgtccaa aattaatcgg aggtgcgcgt gcgcactatc
    11401 cgcaagacgc tattagaaag tcttgagaat gaataaagga gataaaagtg tttaaaaaag
    11461 accgtttttc aattcgtaag attaagggag ttgtaggctc tgtatttctt ggaagccttt
    11521 tgatggctcc ttctgtagtg gacgcagcca cctatcacta tgtaaataaa gagattattt
    11581 cacaagaagc taaagattta attcagacag gaaagcctga caggaatgaa gttgtatatg
    11641 gtttggtgta tcaaaaagat cagttgcctc aaacagggac agaagcatct gttttgacag
    11701 cttttggttt gctgactgtt gggagcttgc ttttaatcta caagagaaag aaaattgcta
    11761 gcgtctttct agttggagct atgggattgg tagttcttcc tagtgcagag gctgtagacc
    11821 cagttgcgac cctagcgctg gctagtcgag agggtgttgt tgaaatggat ggctatcgct
    11881 atgttggtta tctatcaggt gacatcctca aaacgcttgg cttggacact gttttagaag
    11941 aaacctcagc aaaacctgga gaggtgactg tagtcgaagt tgagactcct caatcaacaa
    12001 caaatcagga gcaagctagg acagaaaacc aagtagtaga gacagaggaa gctccaaaag
    12061 aagaagcacc taaaacagaa gaaagtccaa aggaagaacc aaaatcggag gtaaaaccta
    12121 ctgacgacac ccttcctaaa gtagaagagg ggaaagaaga ttcagcagaa ccagctccag
    12181 ttgaagaagt aggtggagaa gttgagtcaa aatcagagga aaaagtagca gttaagccag
    12241 aaagtcaacc atcagacaaa ccagctgagg aatcaaaagt tgaacaagca ggtgaaccag
    12301 tcgcgccaag agaagacgaa aaggcaccag tcgagccaga aaagcaacca gaagctcctg
    12361 aagaagagaa ggctgtagag gaaacaccga aacaagaaga tacacagcca gaagtagtag
    12421 aaacaaaaga tgaggctgct aatcaacctg ttgaagaacc aaaagttgaa acgcctgctg
    12481 tagaaaaaca aacggaacca acagaggaac caaaagttga acaagtaggt gaaccagtcg
    12541 agccaagaga agacgaaaag gcaccagtct ctccagaaaa gcaaccagaa gctcctgaag
    12601 aagagaagac tgcggaagaa acaccgaaac aagaagataa aataaagggt attggtacta
    12661 aagaaccagt tgataaaagt gagttaaata atcaaattga taaagctagt tcagtttctc
    12721 ctactgatta ttctacagca agttacaatg ctcttggacc tgttttagaa actgcaaaag
    12781 gtgtctatgc ttcagagcct gtaaaacagc ctgaggtaaa tagcgagaca aaagctgaaa
    12841 aagttgcagc taatacagat gctaaacaaa gtgaagtaaa ttctgagact gcaagtctta
    12901 aaacagcaat ttctggatta aatacagata aagttgaact cgaaaatcaa cttaagattg
    12961 cacagggaaa aactgaaact gatttcagca tggaatcttg gacggttttg agtacagcta
    13021 aaaataaagc tcaggaagta aaagataacg gaacagcgac ccaagaacaa ataaacgaag
    13081 cagagaaaag tttgaaaaca gcccttgctg acttatctgt tgacaaaaca gctttgggaa
    13141 gcgctatcga tacagctacc aagaaaaata aagagaacta tacaaatcaa acatgggcag
    13201 agttagaaac agttcttaca gcagcaaaat ctgtaaacac taacgagtct aaacaaagtg
    13261 aagttaatga agctgttgaa aaattaactg caactattga aaaattggtt gaattgtctg
    13321 aaaagccacg tttgactcta tctattgaaa agagagacat agatcgaaaa gttacggtca
    13381 cgtatacttt ggaaaatcca gcaaatactc aaataaaatc aataactgca actctgaaaa
    13441 aaggagagga agttgttaag gattttgttt taacagaaga aaatcttaaa actaatcatt
    13501 taactgcttt gtttgaaaag ctagattact acaaagaata cactctatct acggatatgg
    13561 tatataacag aggaaatgat gatgagacgg agagtatttc agaagaacta attcagctga
    13621 atcttaagaa actagaattg aaagatatcc agacagtctc tctgatgaaa tttgaaaatg
    13681 gtcaagaaag tcaggttaca cacttgtctg ataaaccgac cgatttgagt aaattatact
    13741 taaaagttac atcatcaacg tctaaggatg ctgtgttagc cgttagctct atagaagaag
    13801 agattgttga aaataaaaaa atctttaaaa ttcatgcaga tacaccagaa ttagtagttc
    13861 gtaagaaaga tggcagtctt tctaagggat ttgactatta catggagaga gttatccctc
    13921 atgatggtga tatttattat gattttaaag acttgatttc agctatgaca tctaatccta
    13981 caggtacgtt tattttaggg agagatattt cctcgcgtaa tgtaaaaccg gatggaaatg
    14041 gaaaatctta tatcaaaggt gaatttaaag gaaaactact tggtacaaat gataatgtta
    14101 gacactcgat ctttgactta gaatatccat tgtttgatac tataaaatca ggagtcgtga
    14161 aagatattga tttcaagcat gtcaatatgg tattcccaga tagcaatcaa ggagataacg
    14221 tagcaacaat tgcaagagta atcaaagata agacaaaaat tgagaatgtt aatgttgaag
    14281 gttatctcga gggtcgagat catgtagctg gtttagttaa taatcttgaa ggtaattccg
    14341 aaattgaaaa tatatccttt acaggaaaga ttaagtctaa aggtggtaat tctattacag
    14401 caggtattgc aggtagaaat atcttatctc gtgttaaacg tgcctatgta aatgcaaata
    14461 ttgaagtcct tggttcaacg aattcgagta tgcttgttgc agtaaatggt acaacattga
    14521 atgctagtgg aggttgggga gcctggggaa gacttactga gtccgttgct aaagggacgt
    14581 tagaaattaa acgcagtgga caagctggtg gagttactgc aacagtctgg ccttatggag
    14641 caattgataa ggttgtcagc tatgcaaaag ttaccaaagg aaaagaatta tttgggtcag
    14701 atggtgattt aaataataat tggtttatgc aaaaaattaa taatattttt ggtgttcaag
    14761 gtatcagctc aggtgactca gggaatgatt ctaagtttaa gcgtatttcc gaggaagaag
    14821 caaagcaaaa ggttgcctct tataatatta cagctcccaa cttgatgagc gactcaagtc
    14881 ttttggtaga cagactcaat gaaagttgga aaaatactga tcaatttgaa agtatacagg
    14941 attatcaatc ccaaaaccaa ttaatttacc agaacttgac taaatttact ccttattaca
    15001 ataaagaatt tattgttcat gaaggaaatg ctctcactcc tgaacaggaa atattgaaga
    15061 ctaagaaaat caaatcgatt gtagggttga aaggaacaga gttcgttgta gatggcagtg
    15121 atattgatac tatcatgctt cattttgagg atggtagtca gaagcgatat aaggtaacct
    15181 ctactggtaa gttttcgata actaatttac cggaatatca ggttgaagat ttaaatgtgg
    15241 tgtatacatc agaacatata gtacatccac tagattcgtc actgataaat aaccttgttg
    15301 aagaattgaa aaaagtagaa ttgtatacag agtctactta ccaagttcta ggtattgata
    15361 aagataatgc taacaaattg aatagaacta agagattatt cttagatgag tcattagatg
    15421 ctgttaaaac acaattgcct acctttgtaa aaacaatgtt tgaaaatgag tggttgcaca
    15481 tcaatggaga atctagtgga gctgtcgctg ctttacgaca aaaaatcatg gataataaga
    15541 cggctatatt gcttgctttg acttatatca atcgttatta tgatgttaaa ttctcagact
    15601 ataatattaa aaaactgatg ctctttaaac caactttcca tggtgaaaag attgatctat
    15661 tagatcgatt aatccgacta ggatcatctg gagaaaatag attaaaaggt tcagaaaatg
    15721 cagaaacgtt caagcagtta tttgctagcg aaacgaagca gaaggatttg gtgacttatt
    15781 tagactacaa tcgctctttg ttgactaatt atcaaacaac aggggaatgg tttaaagaaa
    15841 cgactaagga ttatattcaa tttgaggaac gtccatcctt agtagaggag attaaagatg
    15901 ccaaatatcg agtttacgat aatttaaccg caccgtatta tcaaggatat atcctacctc
    15961 tcttaacgtt gaagaatact catttagcaa ttttatcgaa ctatagtact atgacatttg
    16021 tcagccgtga aaaacgccct aattggaaaa atgaagattt tgataagtgg gtgaaatatg
    16081 tagcaactgc tcatagaaac catgttgata cttggtataa gattttacct gataatatca
    16141 aaggtaaaat ggttaaagag aatgttactg ctgtttggga aggattgagc atcccaggaa
    16201 gtgaatgggt agaccagaat gctgtagata gaaaaggaag agattacgcc ccagctcgag
    16261 aattctttaa tcttgtaggt ggtcctatgg gaggttggta tgcttaccat ggttatggtg
    16321 cgcatgctgg aggaagaaat cgggttaatt atgaggtgtt tgatgtatta agtgagtatg
    16381 gtatatcggt ctttactcat gaattgactc acgttaatga tacttggatt tacttaggag
    16441 ggtatggtag acgtgaaaat atgggacctg aagcttatgc tcagggatta ttccagtctc
    16501 ctgttccagg tcagccgggt tggggggcgc ttggtctcaa tatggcattt gaaagaaaaa
    16561 atgatggtga tctaatttat aatgcctccc caactcaatt tgaaaatagg aaagagttag
    16621 atagttatat gaaaaattat aacgatactc tcatgatggt ggactacttg gaaggagatg
    16681 cagttatctc taaaggtaaa gaagctatta ctaaatggtt taaaaaagtt gaacctaagg
    16741 ttgttagtca aacagctcag tatgatacgg ttcgtcagtt gactgctgaa gagaaagaaa
    16801 aactttctgt ttctagtgtt gatgatttag ttgatcaagg attaatgtca gacagagctg
    16861 taggaaataa tacctataat ccagctgatt ttgaaacaag ttatattgcg atagattata
    16921 tgactggtat ttatggtgga ggaaagaata gtgtaggttc accaggtgcc ttgatgttta
    16981 aacataatac tttccgtatg tggggctact atggttttga agaaggtgtt ctaggatatg
    17041 cttctaataa atttaagcaa gcatctagag atgaaggtca tgctggactt agtgataact
    17101 ttattatttc taagatttcc aaaggggaat ttttaactat ggaagcattt aaaaagggat
    17161 attttaagaa agtagtagaa gagttaaaaa ctaaaggtat tcgtcctgtt actatcaatc
    17221 aaaaaacata tagcaccttt gaagaattgc aagaaggatt taagcaggct gttgaaagag
    17281 atttgaagaa aaatcagtta gatgagaggg aaacaagaaa cttcaaattc caagttttca
    17341 gacaacttct tcaacaaaca gatagcttta aaacttctat ctttagataa tcgaaatctc
    17401 tcatctgctt tgcggatgag ggttttctta ttttatgcta tacttaaggt atgcatagaa
    17461 aaacagtgat tgattttagg actttagggg agagatatac ctttacccag cctattaaag
    17521 agttgaaaac gagaaatgta gaagaagtgg cagatttgct ggcacaagtg gaaagctacc
    17581 aagagcaagg ttattatgtg gtggggtatg tcagctacga ggctgcacct gcttttgagg
    17641 aaaaattagc agttcacaag gatcccctac tgggagagta cctgctttat tttactgttc
    17701 atgatagcgt agaaacttcc cctattcctc tgacttatga ggatattgat ttgccctcaa
    17761 attggcagga agtaacgtct gcagcagact atgaaaaggc cattgcccag atacaccatc
    17821 acttgaggca gggagacacc tatcaggtca actacaccgt ccaactcaag caaaagttaa
    17881 gtgccaatcc ttttgccatc tacaatcgta tggtggtaga gcaggaggcg ggctacaatg
    17941 cctatgtgga acatgacgag atggcagtga tttccatgag cccagagctc ttttttgagc
    18001 aaaatgatcg cgagttgaca acacgaccaa tgaaggggac gactcagcgt ggggtaactg
    18061 accaagaaga tcttgaacag gccagttggt tggaacagga tcccaaaaat cgctctgaaa
    18121 atatgatgat tgtggacctc ttgcgcaatg atatgaaccg tatttctgaa gttgggagcg
    18181 agcacgtgga gcgtctgtgc caggtagagc agtattcaac tgtttggcag atgacttcga
    18241 ccatcaagag tcagttgcga gaggatgtgg accttgttga aatcttccgc tcactctttc
    18301 cttgtggttc cataacgggt gcaccgaaaa ttgcgacaat ggagattatc aagaacttgg
    18361 agcctcaacc gcgtggagtc tactgtggaa cgattggtct cttgcttcca aatggacgac
    18421 ggatttttaa tgtggccatt cgtaccattc aacttcacaa aggtcaagcc atttatggag
    18481 ttggcggagg gattacttgg gatagcacat gggaatctga ataccgagag gttcatcaaa
    18541 aggcagcagt tctttatcgt aaacaagctc gcttccaact gattacgaca ggggaaatca
    18601 gccagaagaa cctgctgttt gaagatcaac atctggaaag actgagaaaa gctagtcgtt
    18661 attttgcctt tccttttgat gcagaagact tgggacacaa gattgaggaa gagtgtcagg
    18721 attgtgaagc taatcaagat taccgcttgc gaatcagcct tagcaaatct ggagagatag
    18781 aagtcaatcg tcaagtatta acccctctca gtacaagctt ttgtcaggcc caagtctgcc
    18841 ttcaggaagc tgctttgaat caatccttta cctactttaa aaccactcac cgaccgcatt
    18901 tgagcctagg agaacaagag aagatttacc acaataagtc aggagaactg cttgaaacct
    18961 ctataggaaa tttggttctg aaaatcgctg gaaaactcta cacaccgcct atccgacttg
    19021 gaatcttgcc aggaatttac cgtcagcatt tgctagaaac aggacaggta gaagagaaag
    19081 tcttgacctt ggcagattta acccaagcag aagctattta cggctgtaat gcagtgagag
    19141 gcttgtatga actaagcctt gaggagaact agatgaaatt gatatttttg catgggttag
    19201 ggcagtcagc agagtcttgg aaagaagttc ggaatctact gacagattat ccttctgagg
    19261 ctatcgagtt atttccttct ggagttagta actatcaaca agctaaagag cgagtttatc
    19321 agcacctagc tcaagagaca gaaccttttg ttttgattgg attgtcctta ggtgctgccc
    19381 tgacactaga actttccagt tacgatttac caaatcttcg agctttgatt ctgtcaggat
    19441 gcccgcttaa attagcaggg aatatcctat tttatcttca gttgctgatt tttaaactac
    19501 ttcccaaaag agtatttgaa aaacagggag cagacaagac tcttatggtc ggagtttctg
    19561 aggaattgaa gacacttgat ttaacggata tagcagggac ttgctcctat ccaaccttac
    19621 taatttgtgg cagcaaagat aaaccgaatc ttagttttat gaaagctctc catagactgt
    19681 taacagattc gcagtttcag atcattcctg atggccctca tgtcttgaat aaggcaaaac
    19741 caaaggagtt tgtagaaaag accagaagtt tccttgaatt gctgaaataa gtttgataaa
    19801 ataatattga aatcgatatc ataattatgg gagaaagaaa tgaataaacg tctattttta
    19861 aaaatgagtc tggtgacgtt gccaatttta gccttgtttt cacaaccagt tttggcggaa
    19921 gaaaacatcc atttttcgag ctgtaaggaa gcttgggcga atggctattc ggatattcac
    19981 gagagagaac ctggttattc tgccaagtta gaccgtgatc atgatggtgt ggcttgcgaa
    20041 ttgaaaaatg ctcctaaggg tgcttttaaa gcaaaacagt caacggctat tcaaatcaac
    20101 acaagttcag caacaacaag tggttgggtt aagcaggacg gcgcttggta ctactttgat
    20161 ggaaatggaa atctagtgaa aaatgcatgg cagggaagct attacctgaa agctgatggt
    20221 aaaatggcac agagtgaatg gatttatgac tcttcttatc aagcttggta ttacttgaaa
    20281 tcagatggtt catatgctcg caatgcatgg caaggaaact actatttgaa atcagatggt
    20341 aaaatggcta aaggtgaatg ggtttatgat gccacctatc aagcttggta ttatttgaca
    20401 tcagatggtt cttatgctta cagtacatgg caaggaaatt actatctaaa atcggatggt
    20461 aaaatggctg tcaatgaatg ggttgatggt ggacgttatt atgttggcgc tgacggagtt
    20521 tggaaggaag gtcaagcaag tacagtttct tctagtaatg atagcaatag tgaatattct
    20581 gctgctttag gaaaggcaaa aagttataat tcgttattcc acatgtcaaa aaaacgtatg
    20641 tatagacaat taacttctga ttttgataaa ttttcaaatg atgcagctca atatgccatt
    20701 gatcatttag atgattaaca gatactggta ggcgaaaaaa ttccttataa ttcatttacg
    20761 caaacctttg cataagtgaa tggattttga tatactagta ctgtaagcat tttcaattac
    20821 tacacaatga aaaaggagaa tatgatgagt caaaagatta ttgggattga ccttggtgga
    20881 acttctatca aatttgcaat cttaacaaca gcaggagaaa tccaaggaaa atggtcaatc
    20941 aagaccaaca ttttggatga gggaagtcat atcgttgatg atatgattga gtctattcaa
    21001 catcgtttgg acttgcttgg attggcagca gcggacttcc aaggcattgg aatgggatca
    21061 ccaggtgtgg ttgaccgtga caaagggact gttatcggtg cctacaactt gaactggaaa
    21121 acccttcaac caatcaaaca aaagattgaa aaagctttgg gcattccatt tttcatcgat
    21181 aatgatgcca acgtagcagc tcttggtgag cgctggatgg gtgctggaga taaccaacca
    21241 gacgttgtct ttatgacact cggtactggt gttggtggtg gtattgtcgc agaaggcaaa
    21301 ttgcttcatg gtgttgctgg tgcagcaggt gaacttggtc atatcactgt tgactttgac
    21361 caaccaatct catgtacttg tggtaaaaaa ggctgccttg agacagttgc ttcagcaaca
    21421 gggattgtca acttgactcg tcgctatgcc gatgaatacg aaggcgatgc agccttgaaa
    21481 cgcttgattg ataacggaga agaagtaact gctaagactg tctttgatct cgcaaaagaa
    21541 ggagacgacc ttgctttgat tgtttatcgt aacttctcac gttacttggg aatcgcttgt
    21601 gctaacattg gttcaatcct aaacccatca acaatcgtca tcggtggtgg tgtgtcagct
    21661 gcgggagaat tccttctaca aggtgttcaa aaagtttacg atgaaaatag tttcccacaa
    21721 gtacgcacat ctactaaatt agctcttgca actctaggaa atgacgctgg agttatcgga
    21781 gcagcatcac ttgtattgca ataagataat gagagaggaa aatgctaacc tagagttagt
    21841 aatgttcctc ttttattttt tatgctatac tagttaggac aatgaatgag gtgagagtaa
    21901 atgacaaaag cagatacgat ttttaaagag aatattgaac gcattctaaa ggaaggtgtt
    21961 ttctcagagc aggctcgtcc taagtacaag gatgggactg ttgctaattc caagtatgta
    22021 actggtgcct tttccgagta cgacttatct aaaggagaat ttcctatcac aaccttgcgt
    22081 cccattgcca tcaaatccgc catcaaagaa gttctctgga tttaccaaga ccagtcaaat
    22141 agcttagaag tgcttaatga caagtacaat gttcactact ggaatgactg ggaagttgga
    22201 gacacgggaa ccattggtga gcgctatggt gccgttgtta agaaacacga cattatcaat
    22261 aagcttctca aacagttgga aaccaatcct tggaaccgcc gcaatattat ttcgctctgg
    22321 gattaccaag ctttcgaaga aacagatggg ctgctcccgt gcgcctttca gaccatgttt
    22381 gatgtccgcc gtatagatgg agaaatctat ctggatgcga ccttgaccca gcgctcgaat
    22441 gatatgctgg tggcccacca catcaacgct atgcagtatg tggctttgca gatgatgatt
    22501 gccaaacatt ttggctggaa ggttgggaag ttcttctact tcatcaacaa cctccatatc
    22561 tatgataatc aatttgaaca agctcaggaa ttgctccgtc gggagccgtc aaactgccaa
    22621 ccacgcttgg ttttaaatgt tcctgatggg actaatttct ttgatatcaa agcagaagat
    22681 tttgagttgg tggattatga ccctgttaag ccacagttga agtttgacct agctatttaa
    22741 aagaatagaa aaaagaagtt gagaataatc ccaacttctt ttgtttctta acgtgatacg
    22801 cggcgacgag ctgctttttt acggttttct tcgatgaaag ctgctttttg ctcttctggt
    22861 tcgattactt tctttttaaa tgcgtatact gcacctgcaa cggcagcgac agttcctgcg
    22921 acacctgtta caagaccttt agcgaatcct ttagccatga gtcttcctcc tttatattct
    22981 caatcagcca gcctcctcaa gaggtcacat ttttctgact gacctttttg tgttataata
    23041 atagtaacga aaaaatggga atttttcaag gaaaaaagat gaaaacaaaa ataattgtga
    23101 ttgttggacc gactgctgtt ggaaagacgg ctctagctat tgaagttgca aagcgtttta
    23161 atggcgaagt ggttagtgga gatagccagc aagtctatcg aggacttgat attgggacgg
    23221 ccaaggctag tccagaagag caggcagctg ttcctcatca tttaatcgat gttagagaga
    23281 taaccgagtc ttactcggct tttgattttg tttcagaagc taagatgact attgagggta
    23341 ttcacaaccg tggcaagcta gccattatcg ccggtgggac tggactttat atccagagct
    23401 tgctagaagg ttaccaccta ggtggggaga ctcctcatga ggagatttta gcttatcgag
    23461 ctagtttgga gccatattca gatgaggaat tagcccattt ggtggatcaa gcaggccttg
    23521 agattcccca gtttaatcgt cgtcgtgcta tgcgtgcctt agaaattgcc cattttggtc
    23581 aggatttgga aaatcaagag accttgtatg aaccgctgat tatctgcttg gatgatgaac
    23641 gtagtcaact ttatgagcgt atcaaccacc gagtggattt gatgtttgag gctgggcttt
    23701 tggatgaggc caagtggctg tttgaccatt cccctaatgt acaggctgct aaaggaattg
    23761 gctataagga actctttcct tatttccgtg gagagcagac cttggaggaa gctagtgaga
    23821 gtctcaaaca ggcgacccgt cgttttgcca agcgtcagct gacctggttc cgtaatcgca
    23881 tgcaggtcac cttttatcag attggagaat ctggtgtaca agaccgcatt ttaagccaga
    23941 tagaggagtt tttagatgat tgaaacggag aaaaaagagg agcgagtcct gctgattggt
    24001 gtggaattgc agggtatgga cagttttgac ctctccatgg aagaattggc tagtttagcg
    24061 aaaacggcag gggcagtcgt tgtagatagc tacagacaaa aacgtgaaaa atatgattcc
    24121 aagaccttcg tcggctctgg taagttggaa gagattgcgc ttatggtgga tgcagaagaa
    24181 atcactactg tcatcgtcaa caaccgtctg accccaaggc agaatgtcaa tctagaggaa
    24241 gttctcggtg ttaaggtcat tgaccgtatg cagttgattt tggatatctt tgccatgcgg
    24301 gctcgaagcc atgaagggaa gctccaagtc cacctagccc aactcaaata ccttttgcct
    24361 cgcttggttg gtcaggggat tatgctcagc cgtcaggcag ggggaattgg ttcccgtggt
    24421 cctggtgaaa gccagctgga gctgaaccgt cgtagcgttc gcaatcaaat cacggatatt
    24481 gagcgccagc tcaaggtggt tgagaaaaat cgggcgactg tcagagaaaa acgtttggag
    24541 tctagcactt ttaagattgg tttgattggt tatactaatg ctgggaaatc aactatcatg
    24601 aacatcttga ccagtaagac ccagtatgaa gcagatgagc tctttgcgac tctggatgcg
    24661 acaaccaaga gtattcatct gggaggcaat ctccaagtaa ctttgacaga taccgttggc
    24721 tttatccaag atttgccgac agagttggtg tccagtttca agtcaacctt ggaagaaagc
    24781 aagcatgtgg accttctggt tcatgttatc gatgctagca atccttacca cgaggagcat
    24841 gaaaaaacgg ttctctccat catgaaagac ctggacatgg aagatattcc tcacttgacg
    24901 ctttataata aagcggattt ggtggaggat ttcacgccta cccaaacgcc atataccctc
    24961 atttctgcca agtctgagga cagtcgtgaa aacttgcaag cattattgct agataagatt
    25021 aaggaaattt ttgaagcatt taccctgcga gtgccttttt caaagtccta caagattcat
    25081 gatttagaga gtgttgcaat tctggaagaa cgtgattatc aggaagacgg cgaagtgatt
    25141 acaggctaca tttccgagaa aaataaatgg aggttagaag aattttatga ctgatattaa
    25201 aactttggct ctaaagtatg gaggttatac aagtctggac aaggtctatc tggatcagct
    25261 tctagctggt aaaacagagc aggagcagtt aacacttatt acacctccgc caagtgttgt
    25321 taatgcctac tttgcagaac tctatcagaa aaaaagtcct caggctgcga cggattattt
    25381 tgcagaactc agtcaggaac tgaatcttta caatactgag ccaagtttca ccctagaaaa
    25441 taagcctttt attcggctta atctgtctgg taaatccttt ggtttttgtt atgagagtga
    25501 cggctttggt cggattttct ctgaaaatga ggagaaaatc tcggatgact tgctctttga
    25561 aattgcgcaa attttctccc atcaattagt ctttgaagag tctggtaaga tttacatgaa
    25621 gcctgtcaag gacgaggaag ttgtcagcct agaaagtctc acagctttga ctgatttgga
    25681 aagattggct gatggtcgaa aacgtctcaa aggctacagc caagaggatt tattgcaaga
    25741 agctgctgct ttttctggca aacgctattt ccgatcggaa aaccgcacag ccatgttata
    25801 tattgattaa ttagaaagta tcgaatggat attcaatttt taggaacggg ggctggtcag
    25861 ccctctaaag cccgcaacgt ttcaagtctc gccctgaaac ttttggacga gattaacgaa
    25921 gtttggctct ttgactgtgg agaaggtacg caaaatcgca ttctggaaac cacaattcga
    25981 ccacgtaagg tcagcaaaat ctttattact catctgcatg gagaccacat ttttggtttg
    26041 ccaggtttcc tttctagccg tgcctttcag gccaatgaag agcagacaga tttggaaatc
    26101 tacggacctc aaggaatcaa gtcatttgtc ttaaccagcc ttcgtgtgtc aggttctcgt
    26161 ctgccctacc gcattcattt ccatgagttt gaccaagatt ctctgggtaa aattcttgaa
    26221 accgataaat tcactgtgta tgcagaggag ctggaccaca ctattttctg tgttggctat
    26281 cgtgtcatgc aaaaggatct agaagggacg ctggatgctg aaaaactcaa ggctgctggt
    26341 gttccgttcg gcccgctttt tggtaaaatc aaaaacggcc aggatcttgt tttggaagac
    26401 ggaactgaaa tcaaggcagc agactatatc tcagcgccac gtccaggtaa gattatcact
    26461 attttaggag acactcgaaa aacggatgcc agtgtgcgtc tggctgtcaa tgcagatgtc
    26521 ctagttcatg agtccactta tggcaagggt gatgaaaaaa ttgctcgtaa ccatggtcac
    26581 tcaactaata tgcaagctgc acaagtagcg gtagaagcag gtgccaaacg cctcctactc
    26641 aaccatatca gtgcccgttt cctctcaaaa gatataagca aactcaagaa ggacgctgcc
    26701 acaatttttg aaaatgtcca tgtggtcaaa gacttggaag aagtggaaat ctagcagtca
    26761 cagaaaggat aagtatgcct actattctca ttaccggagc tagcggtggt ctagctcaag
    26821 aaatggtcaa actcctgccc aatgaccaac tcatcttgct tggtagaaat aaggaaaaat
    26881 tagcccaact ctacggaaat tattcccatg cagaattgat tgaaattgat attaccgacg
    26941 attcagccct agaagctctg gtaactgatc tttatctccg ctatggcaag attgatgtct
    27001 tgattaacaa cgctggttac gggatttttg agggatttga ccagattgct gataaagata
    27061 ttcaccagat gtttgaggtc aatacctttg ccctgatgaa tctgtctcgt caccttgcgg
    27121 ctcgtatgaa ggaaagcagc aaagggcata tcatcaacat cgtcagcatg gcaggtctaa
    27181 tagctactgg caagtctagt ctttactcag cgaccaagtt tgcggctatt ggtttttcaa
    27241 atgctctgcg actcgaactt atgccctatg gagtctatgt gacaacagtc aatccaggac
    27301 caatccgtac aggatttttc gaccaagccg acccagatgg aacttatctt aaatcggttg
    27361 accgcttcct gctagaacca gatgcagtgg ctaaaaagat tgtcaagatt ataggaaaaa
    27421 acaaacgaga actcaatctc ccgattttgt tgaacctagc tcataagttt tataccctct
    27481 ttcctaagct agctgataag ttggcagggg aaacttttaa ttataagtaa aaagaaccaa
    27541 tgtgcaggtt gttgctagcc tacatattgg ttcttatcat ttctcaacta tcaaactgaa
    27601 cttcttctag gaggtagtct ataaagcgtt cgcccatctt agataggctg gttttctcat
    27661 gctggatata gaccagctca atcgggtcat caatatccag tgggatagaa acgatattgt
    27721 ctccgtttag gttgctgttc aaaatccctg tcgcaatggt ataaccatcc aaaccaatca
    27781 agagattaaa gagggtggca cggtcactga ccacaatgga tttcttgtgg tgttcttgag
    27841 aaagaatctc ttctgaaaag tagaaggagt tgtgcgtccc ttggtcatag ctgaggtaag
    27901 ggaaattctc caaatcagac agtttcacct tgtctttctt tgccagaggg ttggtcttgc
    27961 tgacaaagat atgtggttgc gctgtgaaga gatggtgggc tagcaggtga ttgtcatcca
    28021 gcatcttggt taaaacatca cggttgtaac tgtttaagaa gaggaccccg acctcactgc
    28081 ggaagttctt gacgtcgtcg ataatctccc aagtccgagt ttcacgaagg aagagttcgt
    28141 atttctccat atcgcttttc ttgagcaaag agacaaaggc attgaccaca aaggcatagt
    28201 gttgagacga aacgctaaat agttcgcggt gggcgacagg atttttatag cgttcctcca
    28261 gaagctgggt ctgctcgaca acctgacggg cataggagag aaactccatc ccatcacggg
    28321 tcaaggtgat tcccttggga ttgcggataa agatctcaat gcccatttca ttttccaaat
    28381 ctcgcactgc attggagaga cttggctgag tgataaagag ttgcttggct gcctcattca
    28441 tggagccagt ttcgacgatt ttgataatat aatgtaattg ttgaattctc atgtttttat
    28501 tgtaccatac ttgccttaga atcgctaaag aagatgggaa aataaggagc attgtgtgag
    28561 tctcttcttg aaaaaaacac aaaattttgc tagaatgagt cttatgaaaa cattctatga
    28621 tgtgcagcaa ttcctcaaac gatttggtat tattgtttac atgggaaaac gcttatatga
    28681 tattgaactg atgaagttgg aactatctcg gatttacgat gcagggttga tggataaatt
    28741 agactatcta gaggcagaag cagttcttcg cagagagcac aaggtagaat tggattatat
    28801 tgagaaaaat ggagaaaaga actaatggtt acttggattt tgtgggcact tatactagca
    28861 atgttggcgt ggatgggctt taactatctt cgtattcgcc gtgcggctaa aattgtggac
    28921 aatgaggagt ttgaagcctt gattcgtacg ggtcaattga ttgatttgcg cgacccagca
    28981 gaattccaca gaaaacatat ccttggtgca cgcaatattc cttcaagtca gttgaaaact
    29041 agtcttgcag cccttcgtaa agataaacct gtccttctct acgaaaacca acgtgcgcaa
    29101 cgagttacaa atgcagctct ttacttgaaa aaacaaggtt tttctgagat ttatatcctt
    29161 tcttatggct tggattcttg gaaagggaaa gtgaagacta gctaacctta tttccaaatt
    29221 agttgaatga taggatgaag ctattgtttt atagtataat taatttgtat aaaatttaag
    29281 gagttcttag aacatgaaag aaaagaagaa aatccacttt ttgtgggtat cttgtcgatt
    29341 attcttggtt tgttatttcc aattgtaggc ctgattttgg gtatcatagg attggtcttg
    29401 gctatttcat accaaaaaga
//
LOCUS       DAVLYR010000009        27534 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLYR010000009 DAVLYR010000000
VERSION     DAVLYR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.009"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            83..1171
                     /gene="wecB"
                     /locus_tag="VYO63_000380"
     CDS             83..1171
                     /gene="wecB"
                     /locus_tag="VYO63_000380"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV1671362.1"
                     /translation="MKKIMLVFGTRPEAIKMCSLVNELRKQEDMKTVVCVTGQHKEMV
                     SPVLDLFGVQPDYDLEIMKANQNLFSITISILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIANYHFAPTELAKENLIKE
                     GRNNIYVTGNTVIDALTTTVQKDYTHPDLDLNDGNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYDDVKVIYPIHKNPLVRETATEIFGDTERIQIIEPLDVLDFHNFMNHSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKMLLDDPE
                     EYKKMSQASNPYGNGDASKQIVRILRGI"
     gene            1210..2079
                     /gene="rfbA"
                     /locus_tag="VYO63_000381"
     CDS             1210..2079
                     /gene="rfbA"
                     /locus_tag="VYO63_000381"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1671363.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            2080..2676
                     /locus_tag="VYO63_000382"
     CDS             2080..2676
                     /locus_tag="VYO63_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1671364.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            2686..3735
                     /gene="rfbB"
                     /locus_tag="VYO63_000383"
     CDS             2686..3735
                     /gene="rfbB"
                     /locus_tag="VYO63_000383"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1671365.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            3801..4652
                     /gene="rfbD"
                     /locus_tag="VYO63_000384"
     CDS             3801..4652
                     /gene="rfbD"
                     /locus_tag="VYO63_000384"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1671366.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVARASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            4728..5162
                     /locus_tag="VYO63_000385"
     CDS             4728..5162
                     /locus_tag="VYO63_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1671367.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTF
                     LSGKAENQVITLYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            5235..5492
                     /locus_tag="VYO63_000386"
     CDS             5235..5492
                     /locus_tag="VYO63_000386"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1671368.1"
                     /translation="MVNCEPLEAYRQLEEAELVGCWAHVRRKFFEVPPKQADKSSLGA
                     KGLAYCDQLFSLERDWEALPADERLQKRQEHLQPLMEDFFA"
     gene            5538..5684
                     /locus_tag="VYO63_000387"
     CDS             5538..5684
                     /locus_tag="VYO63_000387"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1671369.1"
                     /translation="MEYSLKYEETFKTILKDGHLVLSNNLAERAIKSLVMGRSKRVQW
                     TLLA"
     gene            5755..5913
                     /locus_tag="VYO63_000388"
     CDS             5755..5913
                     /locus_tag="VYO63_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV1671370.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            6112..8094
                     /locus_tag="VYO63_000389"
     CDS             6112..8094
                     /locus_tag="VYO63_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671371.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDGYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVMAPVNEEFLNSKGDDFAKATDPNSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            8394..13712
                     /locus_tag="VYO63_000390"
     CDS             8394..13712
                     /locus_tag="VYO63_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1671372.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQ
                     NDNAGKPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYD
                     KDGWFWEYKTPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTL
                     PAAVNDHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKV
                     TYDTIQSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKIN
                     ETTAEYLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLL
                     SSISFLGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVS
                     TDKLAAGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFP
                     EYTKELPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFG
                     SQAQNPFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDF
                     KTLIEKAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDA
                     AYDLAHGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRF
                     AIEWGHGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAA
                     NYPLLGGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDN
                     GSTYKWTPEMRVELVDANNNKVVVTRKSNDVNSPQYRKRTVTLNGRVIQDGSAYLTPW
                     NWDANGKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVK
                     DGKITLDLLANQPYVFYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEI
                     VKSQGANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKE
                     VTTYTNKSLALNYVKAYAHNTRRDNATVDNTSYFQNMYAFFTTGSDVSNVTLTLSREA
                     GDQATYFDEIRTFENNSSMYGENHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRT
                     HLSEKHDPYTQRGWNGKKVDDVIDGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYR
                     VTFEYEAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTG
                     AETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALK
                     NYLPTVAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTA
                     LVADDFASLTAPAQAQEGLANAFDGNVSSLWHTSWGGGDVGKPATMVLKEPTEITGLR
                     YVPRGSGSNGNLRDVKLVVTDESGKEHTFTATDWPNNNKPKDIDFGKTIKAKKIVLTG
                     TKTYGDGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQA
                     SMKYATDNHLLTERMMEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDY
                     KQEIARPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(13879..16038)
                     /locus_tag="VYO63_000391"
     CDS             complement(13879..16038)
                     /locus_tag="VYO63_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1671373.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(16035..16631)
                     /gene="recU"
                     /locus_tag="VYO63_000392"
     CDS             complement(16035..16631)
                     /gene="recU"
                     /locus_tag="VYO63_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1671374.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            16697..17224
                     /locus_tag="VYO63_000393"
     CDS             16697..17224
                     /locus_tag="VYO63_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1671375.1"
                     /translation="MATALVLGYSTFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            17294..17623
                     /gene="gpsB"
                     /locus_tag="VYO63_000394"
     CDS             17294..17623
                     /gene="gpsB"
                     /locus_tag="VYO63_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1671376.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            17644..18024
                     /gene="rnpB"
                     /locus_tag="VYO63_000395"
     ncRNA           17644..18024
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYO63_000395"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            18109..19266
                     /locus_tag="VYO63_000396"
     CDS             18109..19266
                     /locus_tag="VYO63_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1671377.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFDEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            19279..20673
                     /gene="mapZ"
                     /locus_tag="VYO63_000397"
     CDS             19279..20673
                     /gene="mapZ"
                     /locus_tag="VYO63_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1671378.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            20749..22173
                     /gene="gndA"
                     /locus_tag="VYO63_000398"
     CDS             20749..22173
                     /gene="gndA"
                     /locus_tag="VYO63_000398"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1671379.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            22185..22874
                     /locus_tag="VYO63_000399"
     CDS             22185..22874
                     /locus_tag="VYO63_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1671380.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            22972..23934
                     /gene="cbpC"
                     /locus_tag="VYO63_000400"
     CDS             22972..23934
                     /gene="cbpC"
                     /locus_tag="VYO63_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1671381.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLDPSN
                     GDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            23953..24951
                     /gene="cbpJ"
                     /locus_tag="VYO63_000401"
     CDS             23953..24951
                     /gene="cbpJ"
                     /locus_tag="VYO63_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV1671382.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(25069..26301)
                     /locus_tag="VYO63_000402"
     CDS             complement(25069..26301)
                     /locus_tag="VYO63_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1671383.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEIVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTLFLVLSPFSLI
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(26460..26930)
                     /locus_tag="VYO63_000403"
     CDS             complement(26460..26930)
                     /locus_tag="VYO63_000403"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671384.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            complement(26830..27168)
                     /locus_tag="VYO63_000404"
     CDS             complement(26830..27168)
                     /locus_tag="VYO63_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEV1671385.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
BASE COUNT         8627 a         4902 c         6175 g         7830 t
ORIGIN      
        1 tcataaaaaa atatcataaa aattatatat caatgaaatg gtagattaca tttctacctt
       61 tttatccatt taggaggaaa cgatgaaaaa gataatgcta gttttcggta cacgtccaga
      121 agcaataaaa atgtgttcat tagtcaatga gttgagaaaa caggaagata tgaaaacagt
      181 tgtttgtgta actggtcaac acaaggagat ggttagccct gttttagatt tatttggagt
      241 tcaaccagac tatgatttag aaattatgaa ggctaatcaa aacttgttct ctataacaat
      301 aagtatttta gaaaaaataa aacctgtgtt agaaaaagaa caaccagata ttgttttggt
      361 tcacggtgat actactacga catatgcagc agctttagcg gcattttatc taggaattaa
      421 agttggtcat gttgaagctg gattacgaac ttacaacctg caaagtcctt tcccagaaga
      481 gttcaacaga caatcgactt ctattattgc aaattatcat tttgctccta cagaattagc
      541 taaagaaaat ctaataaaag agggtagaaa taatatctat gttactggaa atacagtgat
      601 tgatgcactt acaactacag tacaaaagga ttatacacac cccgatctag atttaaacga
      661 tggaaatcgc ctcatcttat tgactgctca tagacgcgaa aatctcggag aacctatgag
      721 acatatgttt agagctgtta aacgagtttt aaatgaatat gacgatgtta aagtaattta
      781 tccaattcat aaaaatccat tggtacggga aacagctaca gaaatttttg gagatacaga
      841 acgtattcag atcattgaac ctttagatgt tcttgatttt cacaacttta tgaatcatag
      901 ttatatgatt ctaactgact caggaggggt tcaagaagag gcaccttcgt taggaaagcc
      961 tgtattggtc atgcgagata cgacagaaag acctgaagga gtagctgctg gaacgttgaa
     1021 attggttggg actgatgagg agactattta tcaaaacttt aagatgcttt tagacgatcc
     1081 cgaagaatat aaaaaaatga gtcaagctag taatccttat ggaaatggtg atgctagtaa
     1141 acagattgtt cgaattttac gtggaatttg agtgtgttca gataaagtaa tatagaaagg
     1201 taccctacta tgaaaggtat tattctagca ggtggttcgg ggacacgttt atatcctttg
     1261 actcgcgctg catcaaaaca acttatgccg gtttatgata aaccgatgat ttactaccca
     1321 ctttcaacat tgatgttggc tgggattagg gatattttga ttatttccac tccacaggat
     1381 ttacatcgat tccaagagct tcttcaagac ggatctgagt ttgggctcaa actttcttat
     1441 gcagagcaac caagtccaga tggtttggca caagccttta tcattgggga agagtttatt
     1501 tctgatgata gcgttgcgct gatcttaggt gacaatatct atcacggttc tgggctttcc
     1561 aagatgctac aaaaggcagc gagtaaggag tcgggagcaa ctgtttttgg ctaccatgtc
     1621 aaggatccag agcgctttgg tgtggttgag tttgatcagg atatgaaggc tatttctatt
     1681 gaagaaaagc cagagcaacc tcgttcaaac tatgcagtta caggtctcta tttctatgat
     1741 aatgatgtag tagagattgc caagagtatt aaaccaagtc ctcgtggtga actggaaatt
     1801 acagatgtaa acaaggctta cctagatcgt ggtgatttgt ctgttgagct tatgggacgt
     1861 ggctttgctt ggctggatac tggaactcat gaaagtttac tagaggcttc acagtacatc
     1921 gaaacagtgc aacggatgca aaatgttcag gtagcaaact tagaagaaat tgcttaccgt
     1981 atgggctata tcagtcgaga agatgtattg gccttagccc aaccacttaa gaaaaatgaa
     2041 tacggacagt atctgctccg tttgattgga gaagcataga tgacagataa ttttttcggt
     2101 aagacgcttg cggcacgcaa ggttgaagct attccaggca tgttggagtt tgatatcccc
     2161 gttcatggag ataaccgtgg ctggtttaaa gaaaatttcc aaaaggaaaa aatgcttcca
     2221 cttggatttc cagagtcttt ctttgcagaa ggaaaattgc aaaacaatgt atccttctca
     2281 cgtaaaaatg tccttcgagg tctccacgca gagccttggg ataagtacat ctctgtagca
     2341 gatggaggga aagttctggg ttcttgggtt gatctacgcg agggtgaaac ctttgggaat
     2401 acctatcaga cagtgattga tgcgagtaag ggaatctttg ttcctcgagg cgtagctaat
     2461 ggctttcaag ttttatcaga tacagtgtca tatagctatc tggtcaatga ttactgggct
     2521 cttgaactca aacccaagta tgcctttgtg aactacgctg atccaagcct tggtattgaa
     2581 tgggaaaata ttgcagaagc agaggtttca gaagcagaca aacatcatcc actacttaag
     2641 gatgtaaaac ctttgaaaaa agaagatttg gaataaggaa agaatatgac tgaatacaaa
     2701 aatattatcg tgacaggtgg agctggcttt atcggttcta actttgtcca ttatgtttac
     2761 gagaactttc cagatgttca tgtgacagtc ctagataagt tgacttatgc tggaaaccgc
     2821 gcgaatattg aggaaatttt aggtaatcgt gttgagttag ttgttggtga cattgctgat
     2881 gcggagttgg tagacaagtt ggctgctcaa gcagatgcta tcgttcatta tgcagcggaa
     2941 agccacaatg ataattcgct caatgatcca tcgccattta ttcatactaa cttcatcgga
     3001 acctatactc ttttagaagc tgctcgtaag tatgatattc gcttccacca tgtatcgaca
     3061 gatgaagttt atggggatct ccctttacgc gaagatttgc caggtcatgg tgaaggacca
     3121 ggtgagaaat ttactgctga aacaaaatac aacccaagct ctccgtactc atcaaccaag
     3181 gcagcctcag atttgattgt caaagcctgg gtgcgttctt ttggagtcaa ggcaacgatt
     3241 tccaactgtt caaataacta cggtccttat caacatatcg aaaaattcat cccacgtcag
     3301 attactaaca tcctaagtgg tatcaagcca aaactttacg gtgaaggtaa gaacgttcgt
     3361 gattggattc ataccaatga ccattcttca ggagtttgga caatcttgac aaaagggcaa
     3421 atcggtgaaa cctacttgat tggggctgat ggtgagaaga acaataagga agttttggaa
     3481 cttatcctta aggaaatggg acaagctgcg gatgcctatg atcatgtgac tgaccgtgca
     3541 ggacatgacc ttcgctatgc gattgatgct agcaagctcc gtgatgagtt ggggtggaaa
     3601 cctgaattta ccaactttga agctgggctc aaggcaacaa tcaagtggta tacagataac
     3661 caagaatggt ggaaagcaga gaaagaagct gttgaagcca attatgctaa gactcaggag
     3721 attattacag tataaaaagc aggaaatagc tgctttttat tgctatattg ggaagagtta
     3781 catattagaa aggtctagag atgattttaa ttacaggggc aaatggccaa ttaggaacgg
     3841 aacttcgcta tttattggat gaacgtaatg aagaatacgt ggcagtagat gtggctgaga
     3901 tggacattac cgatgcagaa atggttgaga aagtttttga agaggtgaaa ccgactttag
     3961 tctaccactg tgcagcctac accgctgttg atgcagcaga ggatgaagga aaagagttgg
     4021 acttcgccat caatgtgacg gggacaaaaa atgtcgcaag agcatctgaa aagcatggtg
     4081 caactctagt ttatatttct acggactatg tctttgacgg taagaaacca gttggacaag
     4141 agtgggaagt tgatgaccga ccagatccac agacagaata tggacgcact aagcgtatgg
     4201 gggaagagtt agttgagaag catgtgtcta atttctatat tatccgtact gcctgggtat
     4261 ttggaaatta tggcaaaaac ttcgttttta ccatgcaaaa tcttgcgaaa actcataaga
     4321 ctttaacagt tgtaaatgac cagtacggtc gtccgacttg gactcgtacc ttggctgagt
     4381 tcatgaccta cctagctgaa aatcgtaagg aatttggtta ttatcatttg tcaaatgatg
     4441 cgacagaaga cacaacatgg tatgattttg cagttgaaat tttgaaagat acagatgtcg
     4501 aagtcaagcc agtagattcc agtcaatttc cagccaaagc taaacgtccg ctaaactcaa
     4561 cgatgagcct ggccaaagcc aaagctactg gatttgttat tccaacttgg caagatgcat
     4621 tgcaagaatt ttacaaacaa gaagtgagat aagtagtaga atgattttct agtctaataa
     4681 aagaggcaga taatgaactc caaaggagct taagatgtac gattatcttg ttgttggtgc
     4741 tggtctcttt ggcgcatagc tttggctcag tttctattat cgctcacacc atccatcaga
     4801 agtttaatct gaaggtaccc aattatcgcc aagaagaaga ttgggctagg atgggtttac
     4861 caatcacacg taaggaaatc tctaattggc atatcaaggc aagtcaatac tatttagagt
     4921 ccctttataa ccttttacga gaaaagttgt tagaacaacc tcttcttcat gcggatgaaa
     4981 cctcttatcg ggtcttagaa agtgatagtc agctgaccta ctattggacc tttttgtctg
     5041 ggaaagctga gaatcaagta atcacgcttt accaccatga tcagtgtcgg agtggttcgg
     5101 tagtgcaaga attcctagga gattattctg gctatgtgca ttgtgatatg ttgcggcagt
     5161 aacttaggac tttagtcctc tagttctgtc tatgcgatag cagtccaagg tttaggagca
     5221 aggcgacgct aagcttggta aactgcgaac cgctagaagc ttatcgtcaa ctggaagaag
     5281 ctgaacttgt tggatgttgg gcgcatgtga gaaggaaatt ttttgaagtg ccccccaagc
     5341 aagcagataa atcatcctta ggagctaaag gtttagctta ttgtgatcag ttattttcct
     5401 tggaaagaga ctgggaggct ttgccagctg atgaacgact acagaaacgt caagaacatc
     5461 tccaacccct aatggaagac ttctttgctt agtgccgtcg tcagtcagtt ttatcgggtt
     5521 caaaactagg aagggcaatt gaatacagcc tcaagtatga agaaaccttt aagaccattt
     5581 taaaagacgg acatctggtc ctttccaata atctagctga acgcgccatt aaatcattgg
     5641 ttatgggacg gagtaaaaga gtccagtgga ctcttttagc ctaagctaaa ttttaaaaag
     5701 cgagggtggt tattttctca aagttttgaa ggagctaaag caagagctat tattatgagc
     5761 ttattggaaa cagctaaacg tcatcaacta aatagtgaga aatatctatc ctatcttcta
     5821 gaatgtcttc caaacgagga aactctcgta aacaaagagg ttttagaggc ctatttacca
     5881 tggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg aactatccgt
     5941 gagttcacta atctggagat ttttcaatag acctcgttat tgggcggtta cgatattcat
     6001 attttttgca aagatgttgt ttgaaaaata attttcaaaa attctgaaaa ttctgttgac
     6061 atctttctga aaagagtcta taatggagag aaagttttaa aggagaaaat gatgaaaagt
     6121 tcaaaactac ttgcccttgc gggcgtgaca ttattggcgg cgactacttt agctgcatgc
     6181 tctggatcag gttcaagcac taaaggtgag aagacattct catacattta tgagacagac
     6241 cctgataacc tcaactattt gacaactgct aaggctgcga cagcaaatat taccagtaac
     6301 gtggttgatg gtttgctaga aaatgatcgc tacgggaact ttgtgccgtc tatggctgag
     6361 gattggtctg tatccaagga tggattgact tacacttata ctatccgtaa ggatgcaaaa
     6421 tggtatactt ctgaaggtga agaatacgcg gcagtcaaag ctcaagactt tgtaacagga
     6481 ctaaaatatg ctgctgataa aaaatcagat gctctttacc ttgttcaaga atcaatcaaa
     6541 ggattggatg gctatgtaaa aggggaaatc aaagatttct cacaagtagg aattaaggct
     6601 ctggatgaac agacagttca gtacactttg aacaaaccag aaagcttctg gaattctaag
     6661 acaacaatgg gagtaatggc tccagttaat gaagagtttt tgaattcaaa aggggatgat
     6721 tttgccaaag ctacggatcc aaatagtctc ttgtataacg gtccttattt gttgaaatcc
     6781 attgtgacca aatcttctgt tgaatttgcg aaaaatccga actactggga taaggacaat
     6841 gtgcatattg acaaagttaa attgtcattc tgggatggtc aagataccag caaacctgca
     6901 gaaaacttta aagatggtag ccttacagca gctcgtctct atccaacaag tgcaagtttc
     6961 gcagagcttg agaagagtat gaaggacaat attgtctata ctcaacaaga ctctattacg
     7021 tatttagttg gtacaaatat tgaccgtcag tcctataaat acacatctaa gaccagcgac
     7081 gaacaaaagg catcgactaa aaaggctctc ttaaacaagg atttccgtca ggctattgcc
     7141 tttggttttg atcgtacagc ctatgcctct cagttgaatg gacaaactgg agcaagcaaa
     7201 atcttacgta atatctttgt tccaccaaca tttgttcaag cagatggtaa aaactttggc
     7261 gatatggtca aagagaaatt ggttacttat ggggatgaat ggaaggatgt taatcttgca
     7321 gattctcagg atggtcttta caatccagaa aaagccaagg ctgaatttgc taaagctaaa
     7381 tcagccttac aagcagaagg tgtgactttc ccaattcatt tggatatgcc agttgaccag
     7441 acagcaacta caaaagttca gcgcgtccaa tctatgaaac aatccttgga agcaacttta
     7501 ggagctgata atgtcattat tgatatccaa caactacaaa aagacgaagt aaacaatatt
     7561 acatattttg ctgaaaatgc tgctggcgaa gactgggatt tatcagataa tgtcggttgg
     7621 ggtccagact ttgccgatcc atcaacctac cttgatatca tcaaaccatc tgtaggagaa
     7681 agtactaaaa catatttagg gtttgactca ggggaagata atgtagctgc taaaaaagta
     7741 ggtctatatg actacgaaaa attggttact gaggctggtg atgaggctac agatgttgct
     7801 aaacgctatg ataaatacgc tgcagcccaa gcttggttga cagatagtgc tttgattatt
     7861 ccaactacat ctcgtacagg gcgtccaatc ttgtctaaga tggtaccatt tacaatacca
     7921 tttgcattgt caggaaataa aggtacaagt gaaccaatct tatataaata cttggaactt
     7981 caagacaagg cagtcactgt agatgaatac caaaaagctc aggaaaaatg gatgaaagaa
     8041 aaagaagagt ctaataaaaa ggctcaagaa gatctcgcaa aacatgtgaa ataactgttg
     8101 caaaatataa gaaaggattt agtatttctc ttgaatgctg aatccttttt tacatttgta
     8161 aagaaagatt ctaaatgtac tgacccccaa aagttggagc ctctttttgt cagaatagag
     8221 aaaatttttg ttaattttac ttgtttccta ttgctttctc agttattatt tgttatatta
     8281 aaagtataat tatttttatt tatcagggtt aagcattgca ttttcagagg aaggagtatt
     8341 ttttaaaaag gaaatgtaaa cgcttgctca aaaatgaaag gatttagaag tttatgaata
     8401 aaggattatt tgaaaaacgt tgtaaatata gtattcggaa attttcatta ggtgttgctt
     8461 ctgttatgat tggagctgca ttctttggga caagtccggt tcttgcagat agcgtgcagt
     8521 ctggttccac ggcgaactta ccagctgatt tagctactgc tcttgcaaca gcaaaagaga
     8581 atgatgggcg tgattttgaa gcgcctaagg tgggagaaga ccaaggttct ccagaagtta
     8641 cagatggacc taagacagaa gaagaactat tagcacttga aaaagaaaaa ccggctgaag
     8701 aaaaaccaaa agaggataaa cctgcagctg ctaaacctga aacccctaag acggtaaccc
     8761 ctaagacggt aactcctgaa tggcaaacgg tagagaaaaa agaacaacag ggaacagtca
     8821 ctatccgaga agaaaaaggt gtccgctaca accaactatc ctcaactgct caaaatgata
     8881 acgcaggcaa accagccctg tttgaaaaga agggcttgac cgttgatgcc aatggaaatg
     8941 caactgttga tttaaccttc aaagaagatt ctgaaaaggg caaatcacgc tttggtgtct
     9001 tcttgaaatt taaagatacc aataataatg tttttgtcgg ttatgacaag gatggctggt
     9061 tctgggaata caaaactcca ggtaatagca catggtataa aggcaaccgt gttgctgctc
     9121 ctgaaacagg atcaataaac cgtctttcta tcactctcaa gtcagacggt cagctaaatg
     9181 ccagcaataa cgatgtcaat ctctttgaca cagtgactct accagctgcg gtcaatgacc
     9241 atcttaaaaa tgagaagaag attcttctca aggcgggctc ttatgacgat gagcgaacag
     9301 ttgttagcgt taaaacggat aaccaagagg gggtaaaaac agaggatacc cctgctgaaa
     9361 aagaaacagg tcctgaagtt gatgatagca aggtgactta tgacacgatt cagtctaagg
     9421 tcctcaaagc agtgattgac caagccttcc ctcgtgtcaa ggaatacacc ttgaatggac
     9481 atactttgcc aggacaggtg caacagttca accaagtctt tatcaataac caccgaatca
     9541 cccctgaagt cacttataag aaaatcaatg agacaacagc agagtacttg atgaagcttc
     9601 gcgatgatgc tcacttaatc aatgcggaaa tgacagtacg cttgcaagtt gtggacaatc
     9661 aattgcactt tgatgtgacc aagattgtca accacaatca agtcactcca ggtcaaaaga
     9721 tcgatgacga aagaaaacta ctttcttcta ttagtttcct cggcaatgct ttagtctctg
     9781 tttctagtga tcaaactggt gctaagtttg atggggcaac catgtcaaac aatacacatg
     9841 tcagcggaga tgatcatatc gatgtaacca atccaatgaa ggatttggct aagggttaca
     9901 tgtatggatt tgtttctaca gataagcttg ctgctggtgt ttggagtaac tctcaaaaca
     9961 gctatggtgg tggttcgaat gactggactc gtttgacagc ttataaagaa acagtcggaa
    10021 atgccaacta tgtaggaatc cacagctctg aatggcaatg ggaaaaagct tataagggca
    10081 ttgttttccc agaatacacg aaggaacttc caagtgctaa ggttgttatc actgaagatg
    10141 ccaatgcaga caagaaagtt gattggcagg atggtgccat tgcttatcgt agcattatga
    10201 acaaccctca aggttgggaa aaagttaagg atatcacagc ttaccgtatc gcgatgaact
    10261 ttggttctca agcacaaaat ccattcctta tgaccttgga tggtatcaag aaaatcaatc
    10321 tccacacaga tggtcttggg caaggtgttc tccttaaagg atatggtagt gaaggtcatg
    10381 actctggtca cttgaactat gctgatattg gtaagcgtat cggtggtgtc gaagacttca
    10441 agaccctaat tgagaaggct aagaaatatg gagctcatct aggtatccat gttaacgctt
    10501 cagaaactta tcctgagtct aaatatttca atgaaaaaat tctccgtaag aatccagatg
    10561 gaagctatag ctatggttgg aactggctag atcaaggtat caacattgat gctgcctatg
    10621 acctagctca tggtcgtttg gcacgttggg aagatttgaa gaaaaaactt ggtgacggtc
    10681 tcgactttat ctatgtggac gtttggggta atggtcaatc aggtgataac ggtgcctggg
    10741 ctacccacgt tcttgctaaa gaaattaaca aacaaggctg gcgctttgcg atcgagtggg
    10801 gccatggtgg tgagtacgac tctaccttcc atcactgggc agctgacttg acctacggtg
    10861 gctacaccaa taaaggtatc aacagtgcca tcacccgctt tatccgtaac caccaaaaag
    10921 atgcttgggt aggggactac agaagttatg gtggtgcagc caactatcca ctgctaggtg
    10981 gctacagcat gaaagacttt gaaggctggc aaggaagaag tgactacaat ggctatgtaa
    11041 ctaacttatt tgcccatgac gtcatgacca agtacttcca acacttcact gtaagtaaat
    11101 gggaaaatgg tacaccggtg actatgaccg ataacggtag cacctataaa tggactccag
    11161 aaatgcgagt ggaattggta gatgctaaca ataataaagt agttgtaact cgtaagtcaa
    11221 atgatgtcaa tagtccacaa tatcgcaaac gtacagtaac gctcaacgga cgtgtcatcc
    11281 aagatggttc agcttacttg actccttgga actgggatgc aaatggtaag aaactttcta
    11341 ctgataagga aaagatgtac tacttcaata cgcaggccgg tgcaacaact tggacccttc
    11401 caagtgattg ggcaaagagc aaggtttacc tttacaagct aactgaccaa ggtaagacag
    11461 aagagcaaga actaactgta aaagatggta aaattaccct agatcttcta gcaaatcaac
    11521 catacgtttt ctatcgttcg aaacaaacca atcctgaaat gtcatggagt gaaggcatgc
    11581 acatctatga tcaaggattt aatagcggta ccttgaaaca ctggaccatt tcaggcgatg
    11641 cttctaaggc agaaattgtc aagtctcaag gggcaaacga tatgcttcgt attcaaggaa
    11701 acaaagaaaa agttagtctc actcagaaat taactggttt gaaaccaaat accaagtatg
    11761 ccgtttatgt cggtgtcgat aaccgtagta atgccaaggc gagcatcact gtaaacactg
    11821 gtgaaaaaga agtgactact tataccaata agtctctcgc tctcaactat gttaaggcct
    11881 acgcccacaa tacacgtcgt gacaatgcta cagttgacaa tacaagttac ttccaaaaca
    11941 tgtacgcctt ctttacaact ggatcggacg tatcaaatgt tactcttact ttaagtcgcg
    12001 aagctggtga tcaagcaact tactttgatg aaattcgtac ctttgaaaac aattcaagca
    12061 tgtacggaga aaaccatgat acaggtaaag gcaccttcaa acaagacttt gaaaatgttg
    12121 ctcagggtat cttcccattc gtagtgggtg gtgtcgaagg tgttgaagac aaccgcactc
    12181 acttgtctga aaagcatgat ccatatacac aacgtggttg gaacggtaag aaagttgatg
    12241 atgttatcga tggaaattgg tcactcaaga ccaatggact ggttagccgt cgtaacttgg
    12301 tttaccaaac catcccacaa aacttccgct ttgaagctgg taagacctac cgtgtaacct
    12361 ttgaatacga agcaggttca gataatacct atgcttttgt agtcggtaag ggagaattcc
    12421 agtcaggtcg tcgtggcact caagcaagca acttagaaat gcatgaattg ccaaatactt
    12481 ggacagattc caagaaagcc aagaaggcaa ccttcctcgt gacaggtgca gaaacaggag
    12541 atacttgggt aggtatctac tcaactggaa atgcaagtaa tactcgtggt gattctggtg
    12601 gaaatgccaa cttccgtggt tataacgact tcatgatgga taatcttcaa atcgaagaaa
    12661 ttaccctaac aggtaagatg ttgacagaaa atgctctgaa gaactacttg ccaacggttg
    12721 ccatgactaa ctacaccaaa gagtctatgg atgctttgaa agaggcggtc tttaacctca
    12781 gtcaggccga tgatgatatc agtgtggaag aagcgcgtgc agagattgcc aagattgaag
    12841 ctttgaagaa tgctttggtt cagaagaaaa cggctttggt agcagatgac tttgcaagtc
    12901 ttacagctcc tgctcaggct caagaaggtc ttgcaaatgc ctttgatggc aatgtgtcta
    12961 gtctatggca tacatcatgg ggcggaggag atgtaggcaa gcctgcaacc atggtcttga
    13021 aagaaccaac tgaaatcact ggacttcgtt atgttccacg tggatcaggt tcaaatggta
    13081 acttgcgaga tgtgaaactt gttgtgacag atgagtctgg caaggagcat acctttactg
    13141 caactgattg gccaaataac aacaaaccaa aagatattga ctttggtaag acaatcaagg
    13201 ctaagaaaat tgtccttact ggtaccaaga catacggaga tggtggagat aaataccaat
    13261 ctgcagcgga acttatcttt actcgtccac aggtagcaga aacacctctt gacttgtcag
    13321 gctatgaagc agctttggtt aaggctcaga aattaacaga caaagacaat caagaggaag
    13381 tagctagcgt tcaggcaagc atgaaatatg cgacggataa ccatctcttg acggaaagaa
    13441 tgatggaata ctttgcagat tatctcaacc aattaaaaga ttctgctacg aaaccagatg
    13501 ctccaactgt agagaaacct gagtttaaac ttagatcttt agcttccgag caaggtaaga
    13561 cgccagatta taagcaagaa atagctagac cagaaacacc tgaacaaatc ttgccagcaa
    13621 caggtgagag tcaatctgac acagccctct tcctagcaag tgttagccta gccctatctg
    13681 ctctctttgt agtaaaaacg aagaaagact agtatttagt aaaacctctt aacaagatta
    13741 cggaagcagt ctctatcttt tccaatgagg tttatagtac agaaaaagcc tgagaagata
    13801 tcttctcagg cttttgttaa gcacataaat acaatagtgc tatgaaaaaa tcacccagaa
    13861 aaatctggat gataaatgtt atggttgtgc tggttgagga ttctgatttt gttgatcagg
    13921 ggttgtattt gattgttgcg tattattgtt aggattggta gtcgtactat tatttgtgct
    13981 tggagtggtt gagctagact gtgaagttga actatctgat gatgagcttg aactttcagt
    14041 tgatgggggt tgttgtggag caggtgagct ccacgtagaa cgagcaccat ttttaaatac
    14101 gaattctcca tttctgtaga gcccctctgg tatattccaa tcctctggat tgcttccttc
    14161 agacaggtag gtcatcatag agcggtaaac tttggcagcg accgtaaggc cattgcctac
    14221 aagtggtgtc agacggttag aatagcctgt ccatacagcc attgaatatt tacgcgtata
    14281 gccagcaaat agttcatcag gtgctacaaa ttgagaggtc ttgatgtggt tttcaatttc
    14341 ctcgtctgta tagttagagg ttcctgtttt accagcctga gggagccaag caagataggc
    14401 atttcgtcca gttccataac tcaagactgt tttcatcatg tcggtcatca tataggctgt
    14461 cgtttccttc atggcacgag ttccgacatt agagaactct ttttcactcc catcactaaa
    14521 gacgacttta tggatataca ttggtttata gtaagttcca ccatttgcaa aggcagcgta
    14581 agcagcagcc atcttttcac tacttgctcc atattttttg tctgattcgg ttgtgttact
    14641 tgaaatggca tttgagtagt gaatacttgg gtagtcgatt ccgagaccat ttaggaaagt
    14701 cttggcgcgg ttgagtccga ccttgtttag agtttccacg gctgggacgt ttcgcgattg
    14761 ttgcagggcg tattgcaagg tgatgttgcc aaagtagccc ctatcccagt tataaacagg
    14821 ggtatttgtc ccagggtagt tatagggctc atcgtgaacg atagtagcag ttgaatcgta
    14881 gacaccgtac tccaaggcag gagcatagtc tgtgatcggt ttcatagttg atccccagtc
    14941 gcggtttgtt tctactgctt ggttaattcc gaaggaaaca ttacttgact gatggcgtgc
    15001 tcctagctgg gcaatgactt taccgttaga aacatcaaca atggtagaag cgacttgcaa
    15061 ttcatcgtct ggataggcaa cgtattcgtc tgtattgtaa atatcccaca gatgtttttg
    15121 agcttcttgg tctacatttg tgtagacatc catcccagtt gtgagcaggt tatagcctgt
    15181 ttcttcttcc acttgattga tgacttcctt gaggtaatta tccatgtaag cagggtaatt
    15241 acttgctgat ttgagacttt gtagtccatc agtaattggt gtattgactg ctttctcata
    15301 ctgttcagca gagatgtagc cttgattttt catttcagat aagaccaagt ttcggcggtc
    15361 ttgggctgct tctggatgtg aataggggtc atattggttt ggtgcctgag gcattccagc
    15421 cagcaaggct aactgaggta aacttaaatt attgaggtct ttaccatagt agttttgagc
    15481 tgctgtctgc attccatagt tcccattaga catgtagacc ttatttatat agtaggtcaa
    15541 gatttcttgc ttggttgctt tttgttctaa ctgaatcgct aaccaagctt cctgagcctt
    15601 acgagaaata gtctggtcgg aagtcgaagt tgaaaagtaa gtcaacttaa tcaactgttg
    15661 ggtgagagtt gatccacctt ggagggaatt gctttgcaga ttgcgcaaga aagctcccag
    15721 gatacggatg gtatcaatcc ccctgtggtc gaagaagcga tggtcttcga tagaaacgat
    15781 tgccttaacc aaatctgtgg gaatatcatt agcttgggca ttgacgcggc gttcagaacc
    15841 caagtcagca atgagttgat ttttattgtc gtagatttta ctagaagttg ttgcaactag
    15901 tttactctcg gataggctag gagccttgct aacgtagtag aaaaaaactc ctccgcctaa
    15961 gacaatggct gcgataacca agcttaagaa gctaatgctc agatacttga ttaggcgcag
    16021 aatcgttggt ttgttcatct tgttttacca cctaataaat gttctttgat aacattgaga
    16081 taaggaattt gagggaaggc accagccttg atttcatatc catattctcg aatatattca
    16141 agtggcattg atttttgtcc cttatcttga tgatagaagc gaatcaaatc gaatgccggc
    16201 aataagtagg tttcttgctg agaagaaaag tgaagaagga caaagcagat tccttgttgg
    16261 gcaaggactt gttccatatg ctgaatctga tgtggatgaa aatttttcat cggaatcgca
    16321 cgtttttgtt ttgtttcctt gacttcaaag tcgatgtaat atccattata aacgccagaa
    16381 tagtccgtcg ttgaagcttg tcgaaaatag gcttcaacaa tcttggcacg acttcgttgt
    16441 ggatagtcca cttgtacgat ttgaatagga gttggtttct tatgtataac agccaagccc
    16501 tgagacaaat agtagtcgtt ggtagcattg atcatctttt caaaagacat tcctcgattt
    16561 gcgaaatttt tgggttgaga aagagatgtt tgtctttttt gtgatgaaac tttatgtgga
    16621 tagttgacca taattctcct tattggtaca ataacatcac tttattatac cataatttgc
    16681 acagaaaggg ttaaaaatgg ctacagcttt ggttttaggc tattctactt ttgatttggg
    16741 tttattttct gataaggatc ctcgtcttaa attgattaaa aaagcgatac gtaaagattt
    16801 agaggctatg gcagcagatg gggtgtcttg gcttgtgttt acaggaagtt tgggttttga
    16861 atattgggtt ttagaggttg ctcaggagat gaaaaccgaa tacggtttcc agttggccac
    16921 catttttgct tttgaaaccc atggggaaaa ttggaatgaa ggtaatcaga tgaaactgag
    16981 tcgctttaag caggtagact ttgtcaaata tgcttatcct cgctatgaac acaaggggca
    17041 gttaagagat taccagcagt ttttgctgga aaacacgact agttcctatc ttttttatga
    17101 tgaagaaaat gaaacaaaac tagcttattt ttaccaaaag atgaaaaatc aagaggacta
    17161 ttttataaag agattaacat ttgatcaatt aaatgaactt gctgaaaatt tttccgaaaa
    17221 ttgaagcttt gaccttgatt tttgtttgcc tttttttata taataatact agcaagcgag
    17281 aatggagaga gacatggcaa gtattatttt ttcagcgaaa gatatttttg aacaagagtt
    17341 tggacgtgaa gtccgtggct ataataaagt agaagttgac gagtttttag acgatgtcat
    17401 caaggactat gaaacctatg ctgccttggt caagtcactt cgtcaggaaa ttgcggattt
    17461 gaaggaagaa ttaactcgta aaccgaaacc ttcaccagtt caagcagaac cccttgaagc
    17521 ggcaattaca agttctatga cgaattttga tattttgaaa cgcctgaata gattggaaaa
    17581 agaagttttt ggtaaacaaa ttttagataa ctcagatttt taagtagtta tttgagatgt
    17641 gcaatttttg gataatcgcg tgaggagaat tgcttctcat gaggaaagtc catgctagca
    17701 caggctgtga tgcctgtagt gtttgtgcta ggcgaaacca taagcctagg gacgagaaat
    17761 cgttacggca gttgaaatgg ctaagtcctt ggataggcca gagtaggctt gaaagtgcca
    17821 cagtgacgga gtctttctgg aaacagagag agtggaacgc ggtaaacccc tcaagctagc
    17881 aacccaaatt ttggtcgggg catggagtat gcggaaacga acgtagtatt ctgactgcta
    17941 tcagctagag ctgttagtgg tagacagatg attatcgaag gaagtggtcc tagtcacttc
    18001 tggaacaaaa catggcttat agaaaattgc atataggttg gggctgagaa attttctcaa
    18061 cctcattttt taaagtggac atatagaaag gtcttgcaag actgtaacat gaaaaaagaa
    18121 tttaatttaa ttgcaactgt ggcagcaggg cttgaggctg tcgttggtcg tgaagtgcga
    18181 gagttgggct acgattgtca ggttgaaaat ggacgtgttc gttttcaagg agacgtgaga
    18241 gctattatcg aaaccaacct ttggcttcgg gcagcagatc gtatcaaaat tatcgtagga
    18301 acgttcccag ctaagacttt tgaagagcta tttcagggag ttttcgcttt agattgggaa
    18361 aattatttac cacttggagc tcggttaccg atttcaaaag ctaaatgtgt taagtccaaa
    18421 cttcacaatg agcccagtgt tcaggctatt tctaagaaag ctgttgtcaa gaaattgcag
    18481 aaacactatg ctcgcccaga aggggttcct ctgatggaga atggcccaga gtttaagatt
    18541 gaggtctcta ttctcaaaga tgtggcaact gtcatgattg atacgaccgg gtctagcctc
    18601 tttaaacgtg gttatcgtac cgaaaaaggt ggcgctccta tcaaggaaaa tatggcagca
    18661 gccattttac aactttctaa ctggtatcca gacaagcctt tgattgatcc gacctgtggt
    18721 tcggggactt tctgtattga ggcagttatg attgctagaa agatggcgcc aggtcttcgt
    18781 cgctcttttg catttgatga atggaactgg atcagcgatc gcttgattca agaagtgcgc
    18841 acagaagcgg ctaaaaaagt agaacgtaag cttgagttgg atatcatggg ctgtgatatt
    18901 gatgctcgca tggtggaaat tgctaaggcc aatgctcagg cagctggtgt tgcaggagat
    18961 attactttta agcagatgcg cgtgcaggat ttacgttccg ataaaatcaa tggagtaatc
    19021 atttccaatc cgccttatgg tgaacgtttg tcagatgatg caggagtgac caagctctat
    19081 gctgagatgg ggcaagtatt tgcaccgctg aaaacttgga gcaaatttat cctgactagt
    19141 gatgaagctt ttgaaagcaa gtatggtagc caagcagata agaagcgtaa gttatacaac
    19201 ggaaccttga aagtggatct atatcaatat tttggtcagc gtgtcaaacg gcaagaggta
    19261 aaatagaaag ggatactcat gagtaaaaaa agacgaaatc gtcataaaaa agaaggtcaa
    19321 gaaccgcaat ttgattttga tgaagcaaaa gagctaacag ttggtcaagc tattcgtaaa
    19381 aatgaagaag tggaagcagg agtcttgcct gaggattcca ttttggacaa gtatgttaag
    19441 caacacagag atgaaattga ggcggataag tttgcgactc gtcaatacaa aaaagaggag
    19501 ttcgttgaaa ctcagagtct ggatgattta attcaagaga tgcgtgaggc tgtagagaag
    19561 tcagaagctt cttcggagga agttccatct tctgaagaca tcttactacc cttgcctctg
    19621 gacgatgagg agcaaggctt ggatcctcta ttgctagatg atgaaaatcc aacagaaatg
    19681 actgaagaag tggaagagga gcaaaacctt tctcgtctgg atcaagagga ctcagaaaag
    19741 aaaagtaaaa aaggctttat tttgaccgtt ttggcgcttg tatcagtaat tatttgtgtc
    19801 agtgcttatt atgtctatcg tcaagtggct cgttcgacca aggaaattga aacttctcaa
    19861 tcaactacag ccaatcaatc ggatgtggat gattttaata cactttatga cgccttttac
    19921 acaaatagca ataaaacggc tttgaaaaat agccagtttg ataaactgag tcaactcaag
    19981 actttacttg ataagctgga aggtagtcgt gaacatacgc ttgccaaatc taaatatgat
    20041 agtctagtaa cgcaaatcaa ggccattcaa gatgtcaatg cacaatttga aaaaccagct
    20101 attgtggatg gtgtgttgga taccaatgcc aaagccaaat cggatgctaa atttacggat
    20161 attaaaactg gaaatacgga gcttgataaa gtgctagata aggctatcag tcttggtaag
    20221 agccagcaaa caagtacttc tagctcaagt tcaagtcaaa ctagcagctc aagttcaagt
    20281 caagcaagtt caaatacgac tagtgagcca aaaccaagta gttcaaatga gactagaagt
    20341 agtcgcagtg aagtcaatat gggtctctcg agtgcagggg ttgctgttca aagaagtgcc
    20401 agtcgtgttg cctataatca gtctgctatt gatgatagta ataactctgc ctgggatttt
    20461 gcggatggtg ttttggaaca aattctagca acttcacgtt cacgtggcta tatcactgga
    20521 gaccaatata tccttgaacg tgtcaatatc gttaacggca atggttatta caacctctat
    20581 aagccagatg gaacctatct ctttaccctt aactgtaaga caggctactt tgtcggaaat
    20641 ggcgctggtc atgcggatga cttggactac taagcagtcg ttacaaaatt ctttcttttc
    20701 aaaagtaaaa atgataaaat aaaacaaatg aaacaagagg agtgtcaaat gacaaaagct
    20761 aactttggtg tcgtaggtat ggccgtaatg ggtcgtaacc ttgcccttaa tattgaatca
    20821 cgtggttaca cagttgctat ctacaaccgt agtaaagaaa aaacggaaga tgtgattgct
    20881 tgccatcctg aaaagaattt tgtaccaagc tatgacgttg aaagttttgt aaactcaatc
    20941 gaaaaacctc gtcgtatcat gctgatggtt caagctggac ctggtacaga tgctactatc
    21001 caagcccttc ttccacacct tgacaaaggt gatatcttga ttgacggagg aaacactttc
    21061 tacaaagata ccatccgtcg taatgaagaa ttggcaaact caggtatcaa ctttatcggt
    21121 actggagttt ctggtggtga aaaaggtgcc cttgaaggtc cttctatcat gcctggtgga
    21181 caaaaagagg cctacgaatt ggtttcggat gttcttgaag aaatctcagc taaagcacca
    21241 gaagatggca aaccatgtgt gacttatatc ggtcctgatg gagctggtca ctatgtgaaa
    21301 atggttcaca acggtatcga gtacggtgat atgcaattga tcgcagaaag ctatgacttg
    21361 atgcaacact tgctaggcct ttctgcagag gatatggctg aaatctttac tgagtggaac
    21421 aagggtgaat tggatagcta cttgatcgaa atcacagctg atatcttgag ccgtaaagac
    21481 gatgaagacc aagatggacc aatcgtagac tacatccttg atgctgcagg taacaaggga
    21541 actggtaaat ggacgagcca atcatctctt gaccttggtg taccattgtc actgattact
    21601 gagtcagtgt ttgcacgcta catttcaact tacaaagaag aacgtgtaca tgctagcaag
    21661 gtgcttccaa aaccagctgc cttcaacttt gaaggagaca aggctgaatt gattgaaaaa
    21721 atccgtcaag ccctttactt ctcaaaaatc atttcatacg cacaaggatt tgctcaattg
    21781 cgtgtagcct ctaaagaaaa caactggaac ttgccatttg cagatatcgc atctatctgg
    21841 cgtgatggct gtatcatccg ttctcgtttc ttgcaaaaga ttacagatgc ttacaaccgc
    21901 gatgcagatc ttgccaacct tcttttggac gagtacttct tggatgttac tgctaagtat
    21961 caacaagcag tgcgtgatat cgtagctctt gcggttcaag ctggtgtgcc agtgccaact
    22021 ttctcagcag ctattactta ctttgatagc taccgttcag ctgaccttcc ggctaacttg
    22081 atccaagcac aacgtgacta ctttggtgct cacacttacc aacgtaaaga caaagaagga
    22141 accttccact actcttggta tgacgaaaaa taagtaggtc agccatgggg aaacggattt
    22201 tattacttga gaaagaacga aatctagctc attttttaag tttggaactc cagaaagagc
    22261 agtatcgggt tgatctggta gaggaggggc aaaaagccct ttccatggct cttcagacag
    22321 actatgattt gattttattg aacgttaatc tgggagatat gatggctcag gattttgcag
    22381 aaaaattgag tcgaactaaa cctgcctcag tcatcatgat tttagatcat tgggaagact
    22441 tgcaagaaga gctggaagtt gttcagcgtt ttgcagtttc atacatctat aagccagtct
    22501 ttatcgaaaa tctggtagcg cgtatttcgg cgatcttccg aggtcgggac ttcattgatc
    22561 aacactgcag tctgatgaaa gttccaagga cctaccgcaa tcttaggata gatgttgaac
    22621 atcacacggt ttatcgtggt gaagagatga ttgctctgac ccgccgtgag tatgaccttt
    22681 tggcgacact tatgggaagc aagaaagtat tgactcgtga gcaattgttg gaaagtgttt
    22741 ggaagtatga aagtgcgacc gagacaaata tcgtagatgt ctatatccgc tatctacgga
    22801 gcaagcttga tgttaaagga caaaaaagct acattaaaac cgtgcgtggt gttggttaca
    22861 ccatgcaaga atagaaaagc agttgcagtt gtgtaactgc ttttttgagg agtttttata
    22921 tattgacatg cagtcgtgtc tttgctacaa tcgttttagg aggatatata aatgaaactt
    22981 ttgaaaaaaa tgatgcaagt tctactagca gtctttttct ttggtttgct agctacaaat
    23041 acggtatttg cgaataccac aggtggccga tttgttgata aggataatag aaaatattat
    23101 gtaaaagatg atcataaagc aatctattgg cataaaatag acggtaaaac ttactatttt
    23161 ggtgatattg gagagatggt tgtcggttgg caatacttag aaattcctgg aacaggttat
    23221 cgtgatgatt tatttgataa tcgtccagtc ttcgaaattg gccttcagga gaagtggtac
    23281 tattttggac aagatggtgc tttgctagaa caaacagata aacaagtact agaggcaaaa
    23341 acgtctgaaa atacaggaaa agtatacggt gaacaatatc ctctatctgc tgaaaagaga
    23401 acttattatt ttgataataa ttatgctgta aagacaggct ggatttatga agacggcaat
    23461 tggtattatt taaataagct aggaaatttt ggtgatgatt cttacaatcc actaccaatt
    23521 ggtgaagttg ctaagggttg gactcaagat tttcatgtta ctattgacat tgatagaagc
    23581 aaacctgctc catggtacta cctccgttcc tcaggagcaa tggccactgg ctggtatcag
    23641 gaaggtacca cttggtatta tttagaccag ccaaatggcg atatgaaaac aggttggcaa
    23701 aaccttggga acaaatggta ctatctccgt tcctcaggag ctatggcaac tggttggtat
    23761 caagatggtt caacttggta ctatttagat ccttctaatg gagatatgaa aataggttgg
    23821 acaaaagtaa atggaaaatg gtattatctc aattcaaatg gagcaatggt tacaggtagt
    23881 caaactatcg atggtaaagt ttataacttc gcttcatctg gtgagtggat ttaatgttgg
    23941 aggatatata aaatgaagct tttgaaaaaa actatgcaag ctggactaac agtaattttc
    24001 tttggtttgc tagctacgaa tacagtattt gcagataact ctgaaggatg gcagtttgtc
    24061 caagaaaacg gtagaaccta ttacaaaaag ggggacctca aagaaaccta ctggcgagtg
    24121 attgatggta agtactatta ttttgattct ctatctggag agatggttgt cggctggcaa
    24181 tatatcccgt ttccatctaa aggtagtaca attggtcctt acccaaatgg tatcagatta
    24241 gaaggttttc caaagtcaga gtggtactac tttgataaaa atggagtgct acaagagttt
    24301 gttggttgga aagaattaga gattaaaact caagacagtg ttggaagaaa gtatggggaa
    24361 aaacgtgaaa attcagaaga taaagaagag aagcgttatt acacgaacta ttactttaat
    24421 caaaatcatt ctttagagac aggttggctt tatgatcagt ctaactggta ttatctagct
    24481 aaaacagaaa ttaatggaga aaactatatt ggtggtgaaa gacgtgcagg ttggataaat
    24541 gatgcttcag cttggtacta cctagatcca gcaactggta ttatgcaaac aggttggcaa
    24601 tatctaggta ataagtggta ctacctccgt tcctcaggag caatggccac tggctggtat
    24661 caggaaggta ccacttggta ttatttagac cacccaaatg gcgatatgaa aacaggttgg
    24721 caaaaccttg ggaacaaatg gtactatctc cgttcatcag gagctatggc aactggttgg
    24781 tatcaagatg gttcaacttg gtactaccta aatgcaggta atggagacat gaagacaggt
    24841 tggttccagg tcaatggcaa ctggtactat gcttatagct caggtgcttt ggcagtgaat
    24901 acgaccgtag atggctattc tgtcaactat aatggcgaat gggttcggta atgacagggc
    24961 gcttataatt atattagaac actcgcttaa tcaaagaagt gactttgttg tacttgacct
    25021 gacgattggg gagattaaaa gcatgtgaaa tctacacatg ctttttcgtt agttatgagt
    25081 gttgaacttg actaaccatt gacaaaagat aagagcaatc aaaataagat acaaaaatat
    25141 caatgctaac tgcaccgaaa cgcccgatgc tatggatagg gctaataggg agagcaggga
    25201 cggggcgaga atgtctactg tgctcagagc agagcccacc gtccccatct tgtcactggg
    25261 gatatgggca aagacagctg cttgtaggcg agggctgaga atgccgataa tcgtagaact
    25321 gatgaaactg gtcacgaaaa ttacgatata ggcttgacaa aggatactag ttattagagc
    25381 caatagggaa atctcacaga aaaccaagag gtgcgtgaaa cggatatttt taaatagatt
    25441 actgctggtc atattgccaa ggatagctcc acaagacatg atgatggaga gcagggcgaa
    25501 gagttgccca atgctcagtt gtgctagtgt ctgttggatg aagcggaggg caatcagccg
    25561 aggggacaca tccaagatgg caatcatact ggtcgtcaga aacagtagta tgacggtatc
    25621 ttttaacctc aggagcacag ccagcgactg tttaagatgt tgaaaatatt tcttaaaact
    25681 cagtgctgta tgtgacattt caatcctttt ttcaacctca tacaaggtat gtcgaataac
    25741 atacaggccc aaaaaggcaa tgacaaaagt cagagtgttg ataagggaaa tagtttgaat
    25801 actcataaca ttgataagga atgcgccagc tagattggcg acaatgcgga caaccctcat
    25861 cagagactgc ctgaacccca tggcatcatg caggtcgtcc ttaattacac tgatgtagag
    25921 ggcgttcatc atgtaggcag acaggtagct caaggtgtca gagatgaggt tgatgatgac
    25981 aatcataatg actgaaatta atgaaaaggg cgacaagacg agaaatagtg tcaaaatagc
    26041 gtagagcagg attttgattt tggcaataca gagtgcgtgt ttgactctat tttcttgaaa
    26101 atctcctaga atcccgagaa agagttgcga tagtagggga actatctctg aaatggaaat
    26161 cagtaaaatc gctagcggag cgaaattgta ctgagccaca taattgataa tggcgaggta
    26221 gaaaatactg tcgccgaatg atgaaaaaca gctgtttagc agcaactgtc tatatatgtt
    26281 gtttgtccaa aataatttca tacttttctc cttatgtctg cacatcgaat gggaatttat
    26341 tatttaatag acaccaatat accccctgct tcctattccg aagtcaaaca aatctgcttt
    26401 accactatct ccttgaaaca tcagtaaacc gtgatcatct cgttcagaac atgctaaaat
    26461 tacctgttgg attttttcat cagtaatttg ttttgattgt agacagtggt ttgtagtaag
    26521 ctagttattc cgtggcaata tcctgtcatc atttttagat gatggttaaa aacaaattct
    26581 tgatatttac taataatatc tttgtttccg tcacaatcgt acatacaaag atataagatt
    26641 attccggaaa ttccttcgca ccaagaaagt tgtaaatttc ctaaattttc agtattagaa
    26701 gtaacttttt ctaataattt ttttaattca gcatggaatg tatggagatc attatagaac
    26761 atagaaggtt ctaagacttt agaataggca aataaagcat atgcaattcc acaatatcca
    26821 tgagcaaagc tatagtctaa aatgctttct ttggctgtat ctctttgtag gaaataatga
    26881 taaatttccc catatactat tgtggatgaa agttttaaga gtattgtcat tggtaaattg
    26941 atagtatttt attaatgata ataggacacc agattttccc aatgcaaagt ctatttcctc
    27001 tagagtatca taattctcta ttaatttctt agcggtgacg tttgctaatt gtagatagta
    27061 ttgattttta ctgatagaaa ataaatgtag gagtaaccat agatagcctg catcaccaaa
    27121 tagcagtgag tattgatatt catatgtatg tgtcgttcta attgtcactg ccttcttgat
    27181 gatagagagt attttagaat ccagttctgg ggggtacaat ttatttagag gaaacagtag
    27241 tccagctatt ccgtgttgga atgataaatt acttacgaaa tttctaaatt ctgttgaaga
    27301 agtgatcctt ccttcagaat ttacagtttt atctagtaaa aattgtagaa cactattaca
    27361 ttctgttttg taatcatatt tatgttcctc tattttttta attttgacta aattatttgt
    27421 ttgacttaaa tttagtagac attgtaacca aaatagttgc tcatctgtga gctgaccttg
    27481 agtatgtgat agttctagta attgaatagg atcctttagg tagattcaat caat
//
LOCUS       DAVLYR010000010        26303 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLYR010000010 DAVLYR010000000
VERSION     DAVLYR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.010"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..536
                     /locus_tag="VYO63_000405"
     CDS             <1..536
                     /locus_tag="VYO63_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1671386.1"
                     /translation="EKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHTMVQRIVKPS
                     LKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGAGIAGVAIGM
                     GAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQLRSEEQALH
                     FVPNRNITVVSNFSRTD"
     gene            593..1501
                     /locus_tag="VYO63_000406"
     CDS             593..1501
                     /locus_tag="VYO63_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1671387.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            1453..2043
                     /locus_tag="VYO63_000407"
     CDS             1453..2043
                     /locus_tag="VYO63_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1671388.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            2046..3152
                     /gene="yqeH"
                     /locus_tag="VYO63_000408"
     CDS             2046..3152
                     /gene="yqeH"
                     /locus_tag="VYO63_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1671389.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            3366..3677
                     /gene="yhbY"
                     /locus_tag="VYO63_000409"
     CDS             3366..3677
                     /gene="yhbY"
                     /locus_tag="VYO63_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1671390.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            3729..4358
                     /locus_tag="VYO63_000410"
     CDS             3729..4358
                     /locus_tag="VYO63_000410"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1671391.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVEYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            4358..4951
                     /gene="yqeK"
                     /locus_tag="VYO63_000411"
     CDS             4358..4951
                     /gene="yqeK"
                     /locus_tag="VYO63_000411"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1671392.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVGKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            4952..5452
                     /locus_tag="VYO63_000412"
     CDS             4952..5452
                     /locus_tag="VYO63_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1671393.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            5465..5818
                     /gene="rsfS"
                     /locus_tag="VYO63_000413"
     CDS             5465..5818
                     /gene="rsfS"
                     /locus_tag="VYO63_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1671394.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            5921..6667
                     /locus_tag="VYO63_000414"
     CDS             5921..6667
                     /locus_tag="VYO63_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1671395.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            6677..7774
                     /locus_tag="VYO63_000415"
     CDS             6677..7774
                     /locus_tag="VYO63_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1671396.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVNILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTARSVEELVELVTTKRY
                     TKARVRRLLTYILMQARENVLPEVIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIADQNFGRVPIRIETN"
     gene            8012..8266
                     /locus_tag="VYO63_000416"
     CDS             8012..8266
                     /locus_tag="VYO63_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1671397.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            8270..8524
                     /locus_tag="VYO63_000417"
     CDS             8270..8524
                     /locus_tag="VYO63_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV1671398.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            8715..10328
                     /locus_tag="VYO63_000418"
     CDS             8715..10328
                     /locus_tag="VYO63_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1671399.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            10458..11084
                     /gene="gmk"
                     /locus_tag="VYO63_000419"
     CDS             10458..11084
                     /gene="gmk"
                     /locus_tag="VYO63_000419"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1671400.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            11109..11423
                     /locus_tag="VYO63_000420"
     CDS             11109..11423
                     /locus_tag="VYO63_000420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1671401.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            11489..13885
                     /locus_tag="VYO63_000421"
     CDS             11489..13885
                     /locus_tag="VYO63_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1671402.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQVRILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTRNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            13898..14833
                     /locus_tag="VYO63_000422"
     CDS             13898..14833
                     /locus_tag="VYO63_000422"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1671403.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPNRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            14826..16139
                     /gene="rsmB"
                     /locus_tag="VYO63_000423"
     CDS             14826..16139
                     /gene="rsmB"
                     /locus_tag="VYO63_000423"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1671404.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVALKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFANGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            16154..16894
                     /locus_tag="VYO63_000424"
     CDS             16154..16894
                     /locus_tag="VYO63_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1671405.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            16891..18870
                     /gene="pknB"
                     /locus_tag="VYO63_000425"
     CDS             16891..18870
                     /gene="pknB"
                     /locus_tag="VYO63_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1671406.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            18985..19743
                     /locus_tag="VYO63_000426"
     CDS             18985..19743
                     /locus_tag="VYO63_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1671407.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            19745..20194
                     /locus_tag="VYO63_000427"
     CDS             19745..20194
                     /locus_tag="VYO63_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1671408.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            20256..20882
                     /locus_tag="VYO63_000428"
                     /pseudo
     CDS             20256..20882
                     /locus_tag="VYO63_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            20950..21198
                     /locus_tag="VYO63_000429"
     CDS             20950..21198
                     /locus_tag="VYO63_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671409.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            21329..22501
                     /locus_tag="VYO63_000430"
     CDS             21329..22501
                     /locus_tag="VYO63_000430"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1671410.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            22501..23775
                     /locus_tag="VYO63_000431"
     CDS             22501..23775
                     /locus_tag="VYO63_000431"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1671411.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQDDPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(23811..24776)
                     /locus_tag="VYO63_000432"
     CDS             complement(23811..24776)
                     /locus_tag="VYO63_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1671412.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVTTTGYFLPVTLLPGKSI"
     gene            complement(24757..26211)
                     /locus_tag="VYO63_000433"
     CDS             complement(24757..26211)
                     /locus_tag="VYO63_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1671413.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
BASE COUNT         7750 a         5239 c         6139 g         7175 t
ORIGIN      
        1 ttgaaaagct agatgtgaca accattatcg agaatattct aaccaaggtc atttctcttt
       61 tactgctttt gattgtattt tatattgcta aaaaaatgct tcataccatg gtgcagagaa
      121 ttgtcaaacc ttctctaaaa atgtctcgtc atgatgtcgg acgccaaaaa accatctcac
      181 gtttactaga aaatgtgttt aattatacgc tatatttctt tttactctac tgcattttgt
      241 cgattttagg tttgccagtt tctagtttgc tggctggagc tggtattgct ggggtagcga
      301 ttggtatggg agcccaaggc tttctgtctg atgtcatcaa tggctttttc atcctctttg
      361 aacgtcaact ggatgtggga gatgaggtcg ttctgacaaa tggaccgatt actgtatcgg
      421 gtaaggttgt cagtgtggga attcgtacga cacagcttcg tagcgaggag caagcccttc
      481 actttgtccc taaccgaaat atcacagttg ttagcaattt ctcacgcaca gactagacct
      541 gttattttaa gtaatttgtg gtacaataga gggagtttaa taaggagaaa agatggtttt
      601 agaaaagcag ttgggcaatg gttgtacctg gatagaccta gacctaggaa agttgaataa
      661 actagaagac ctttctgaaa tttacggttt ggacaaggaa accattgaat acgcactgga
      721 tagaaacgag cgcgcccaca tggattacca ccgtgaaagt gggacggtta cctttatcta
      781 taatgtccta gacgtaaaaa aggacaaggc ctactatgag acttttccca tgacctttat
      841 tgtcgagcat cgtcgcctga ttaccattag taataccaag aacgcctatg tcattgaaca
      901 gatgactcgt tatctggaga gccatgacac gctttcgatt tataagtttc tctttgccag
      961 tctggaaatt atcagcaatg cctactatcc tgtcattgag cagatggaca agagtaggga
     1021 tgaggtcaat gacctcttgc gccagcgaac taccaagaaa aacctctttg ccctgtctga
     1081 tttggagact ggtatggttt atctgacggc agctgccaaa caaaatcgga ttttgttaga
     1141 gcatattcaa ggtcatgcct tgtatcgtag ttttgatgag attgagagag aacagtttga
     1201 tgatgccatg attgaggctc atcagctggt atccatgaca gacctaatct ctcagatttt
     1261 acagcagctt tcagcctctt acaacaatat tctaaacaat aatctgaatg acaatttgac
     1321 aaccttgact atcatttcag tcttgctagc tgttttggca gtcgtgacag gctttttcgg
     1381 aatgaatgtt cccttacctt taacagatga gccccatgct tggctctata tcagtttggc
     1441 tagtgcaggt ttgtggattg ttttatcctt gttactaagg aaaattgcga aaaaaagtta
     1501 agaaaaggag ccagaatggc gattgaaaat tatataccag attttgctgt ggaagcagtc
     1561 tatgatctga cagtcccaag cctgcaggcg cagggaatca aggctgtttt ggtcgatttg
     1621 gataataccc tcattgcttg gaacaaccct gatggaacgc cagagatgaa gcaatggcta
     1681 catgaccttc gggacgcggg tattggcatt atcgtagtgt caaataacac caaaaaacgc
     1741 gttcaacgag cagttgagaa atttgggatt gattacgttt actgggcctt gaagcccttc
     1801 acatttggta ttgaccgtgc tatgaaggaa ttccactatg acaaaaagga agtggtcatg
     1861 gttggcgacc agctcatgac agatatacga gcagcccacc gtgcagggat tcggtcaatt
     1921 ttagtcaaac ccttggtcca acatgactca atcaaaacgc agattaaccg aactcgtgag
     1981 cgtcgtgtta tgagaaaaat cactgaaaag tacggaccga ttacatataa aaaaggaatt
     2041 taactatgga agaaattctc tgtattggtt gtggagcaac cattcagacg acagataaag
     2101 ctggtcttgg ttttaccccc cagtcggcac ttgaaaaagg tttggagact ggcgaagtct
     2161 attgccaacg ctgtttccgt ctccgccact acaatgaaat cacagatgtc cagttgacga
     2221 acgatgattt cctcaagctc ttgcacgagg tgggagacag tgatgcctta gtggtcaatg
     2281 tcattgatat ctttgatttt aatggatctg tcatcccagg tttaccacgt ttcgtctcgg
     2341 gcaatgatgt cctcttggta ggaaataaaa aagatatcct tcctaagtca gttaagtctg
     2401 gtaagattag ccagtggctc atgaaacgtg cccatgaaga aggtcttcgt ccagtcgatg
     2461 tggtcctaac ttcagcacaa aataaacatg ccattaagga agtcattgac aagattgaac
     2521 actaccgtaa gggccgcgat gtctatgtgg tcggtgtgac caacgttgga aaatcaactc
     2581 taatcaatgc tattatccaa gaaatcacgg gtgatcagaa tgtcatcact acttcacgct
     2641 tcccaggtac aaccttggac aaaatagaga ttccgcttga cgacggatct tatatttacg
     2701 atacgccggg aattatccac cgccaccaga tggctcacta cttgacggcc aaaaacctca
     2761 agtatgtcag tcctaaaaag gaaatcaagc ctaagaccta tcagcttaat cctgagcaaa
     2821 ccctattttt aggtggtttg ggacgctttg actttatagc aggagaaaag caaggattta
     2881 ctgctttctt tgataatgaa ctcaaactcc atcgtagcaa gcttgaagga gctagtgctt
     2941 tctacgataa gcacctggga actcttctga caccaccaaa tagcaaggaa aaagaagatt
     3001 tcccaaggct agtccagcat gtctttacca ttaaagataa gacagaccta gtcatctcag
     3061 gcctaggctg gattcgtgta acaggcatag caaaagtcgc cgtctgggca ccagaaggcg
     3121 tcgccgtcgt cacacgaaaa gcaattattt aaaacaaaat cgctgagcga acaaagtgag
     3181 ctcatatagc gatagttatt gccgtggtgt gattgccaat ctgtagattg gcaaggggca
     3241 caattgagac caaggctcaa ttgtgaggtc aggaaatcca tttttcaaag atgaaatctt
     3301 tggaaaatta gttccgaccg tccctcacca cctaaaataa ctatccaaaa acaaggaaga
     3361 aaattatgtc attaacatca aaacaacgtg ccttcctcaa cagccaggca cacaccctca
     3421 aacctatcat ccaaatcggg aaaaatggac tcaacgacca aatcaaaacc agcgtccgtc
     3481 aagctcttga tgcgcgtgaa ttaatcaagg ttactctctt acaaaacaca gatgaaaaca
     3541 tccacgaagt agctgaaatt ttggaagaag aaatcggtgt ggatacagtc caaaaaatag
     3601 gacgcatctt gattttgttt aaacaatcta gcaagaaaga aaatcgcaag atttctaaga
     3661 aagtcaaaga aatctaagac cgaaactcca aataactgtt tttatagaga aataaagggg
     3721 actagcctat gacaatcgaa ctattgactc cctttaccaa ggtagagttg gagccagaaa
     3781 tcaaggagaa aaaacgcaaa caagttggga ttttaggggg gaattttaac cctgttcaca
     3841 atgcccatct cattgttgcg gatcaagtac ggcaacagtt gggactggat caagttcttc
     3901 tcatgcctga ataccaacct cctcacgttg ataaaaagga aaccatccct gaacaccatc
     3961 gtctcaagat gcttgagttg gcaattgagg gaattgacgg cctagtcatt gaaaccattg
     4021 agttggagcg caagggtatt tcctacacct acgacaccat gaagattttg acagagaaga
     4081 atccagatac ggattattac tttatcatcg gtgccgacat ggttgagtat ctgcctaagt
     4141 ggtaccgaat tgatgaactg gttgacatgg ttcagtttgt gggggttcag cgtccacgct
     4201 acaaggtagg gacttcctat ccagttatct gggtggacgt accgctcatg gatatctcgt
     4261 ccagcatggt gcgggacttc cttgcccaag gtcggaaacc caactttctc ctacctcagc
     4321 cagtgctaga ctacatcgag aaggaggggc tctactgatg tcctatcaag actatatcaa
     4381 ctgctcccgt gaggccttgt tggaaaaaat ggcagagctt ctacctgaga aacgtctaac
     4441 ccattgtcta ggtgtggagc gtgccgctat ggaattagct cagcgctttg gtgtagatgt
     4501 tgggaaagca agtctagcag gtcttcttca tgactatgct aaaaagttgt caaatcagga
     4561 atttctagcc ttgattgacc gttaccagct agaccctgac ctcaaaaact ggggcaataa
     4621 tgtctggcat ggtatggtcg gaatttacaa aattcaggaa gatttggatt tgcatgattc
     4681 tgaaattctg cgagccattg aaattcatac agtcggagct ggtcaaatga cagacctaga
     4741 taaggtcatc tacgtcgcag actatatcga gcacaatcga gcctttcctg gagtagatgt
     4801 ggcgcgtgag attgcaagtc tatcgctcaa taaggcggtg gcctacgaaa cagctcgtac
     4861 cgtggagtat ctagctcatc agggattccc catctatccc caaacccttg aaacctataa
     4921 cgcctttgtg cactatttga aagaggacta aatgaagacg gcttttataa tcattgatgt
     4981 tcagaatgtt ttagtagaaa ctgggttcca gacaaaaagt ctattggaaa aaatttctta
     5041 tttacaaaac caggctagaa gcaagaatat tgaaattatc tatgttcaac atattgagaa
     5101 ttctgaagct caaacatcag aagattggca gttatctgcg cttttaaatc gaaaacctgc
     5161 tgaaaaggtc tttcagaaga agtataacag tattttcaaa gaaactggct taaaagaata
     5221 cttggataaa caggggattg aaaaattagt tttatgtggt atgcagacag aatattgtgt
     5281 ggatacctct gtcaaggttg cctttgaata tggctatcag cttattgttc cagaaggtgc
     5341 tgtcacaacc tttgatgggg atgacattcc agcagaaacg attaatgaat tttatgagga
     5401 catttgggag gagcactttg cagatgtcct agattacaaa catattttct aaaaagagga
     5461 ttaaatgaac gaaaaagaat tactagaact agtcgtgaaa gcggctgatg agaaacgtgc
     5521 ggaggatatc ctcgcacttg atgtacaaga tttgactagt gtgacggact actttgtcat
     5581 cactagctca atgaatagcc gtcagttgga cgctatcgca gctaatatcc gtgaaaaagt
     5641 agctcaagcg ggctttaaag gtagccatgt cgaaggcgat gcagctggag gctgggtctt
     5701 actggacctc ggtgctgttg tcgtgcatat cttttcagaa gaaatgcgtg cccattataa
     5761 tctagagaag ctatggcatg aggcgaattc agtagatatt tcagaagctc ttgcttagaa
     5821 gatgaactca gttgtagtca actgggtttc tttgcttatt ttagaaaaat tgatagaaag
     5881 gtaaagggct agtggtgttt accatggagc tcttagtatc atgattatgg cgacttatga
     5941 aacctttgcg gcggtctatg atgcggtcat ggacgatagt ttatacgaca aatggacgaa
     6001 tttttctctg cgtcatttgc ctaagaccaa ggagaaaaag aaactcttgg aactggcttg
     6061 tgggacagga attcaatcag tgcgcttctc tcaggctggt tttgatgtga ctggacttga
     6121 cttgagtgcg gatatgttga agattgcgga gaagagagca acttcagcca agcaaaagat
     6181 tgcttttata gaaggtaaca tgctgaattt gtccaaggca gggaaatacg attttgtcac
     6241 gtgttattcg gactctatct gctatatgca ggatgaggtg gaagtagggg acgtctttaa
     6301 ggacgtgtac aatgcgctga atgaagaggg agttttcatc tttgacgtgc actcgaccta
     6361 ccagacagat gaagtattcc ctggctattc ctaccatgaa aatgcggaag attttgccat
     6421 gctttgggat acatatgagg acgaagctcc tcactccatc gtgcatgagc tgactttctt
     6481 tatcaaggag gctgacggtt cctttagtcg ccacgatgaa gtacatgagg agaggaccta
     6541 tgagatcttg acctatgata tcttactgga acaggctgga ttcaagtcct tcaaactcta
     6601 tgcagacttt gaggataagg aaccaacaga aactagcacc cgttggtttt ttgtggcgca
     6661 gaagtaggag attagcatga ccatcacagg tattatcgcg gagttcaatc cttttcataa
     6721 tggccataaa tacctgctgg atcaggcaga gggactgaaa attgttgcca tgtcagggaa
     6781 tttcatgcag cgtggagagc ctgctattgt ggataagtgg acaagggccc agatggcgct
     6841 ggaaaatggc gcagacttgg tagtggaatt gcccttttta gtcagtgttc aggcagctga
     6901 tttctttggt caaggagctg tgaatatctt ggatcgcttg ggcattgata gtctagtttt
     6961 tgggacagag gaagttcgag attatcagaa aatcgctgac ttatacacag agaaaggtgc
     7021 tgagatggag aaatttgtgg aaaatctgcc tgattccctt tcctatcccc agaaaaccca
     7081 agctatgtgg aaggaatttg ctggtcttga tttttcaggt aataccccca atcatgttct
     7141 tgctctggcc tatgccaagg cggttgcagg acgaaatatc aagctgcatc cgattcagcg
     7201 tcagggggca ggttaccatt ctgtgaataa agatgtggac tttgcctcgg cgacagccct
     7261 ccgtcaacat cagaaggacc aagatttctt agaacgcttt atgccttctg ttgccctatt
     7321 tgagcaggcc agtaaggtga tctgggaaga ctattttccc ttgctccgct atcaaatctt
     7381 gtcaaatccc gacctaacca ccatctatca ggtcaatcaa gaaatggctg tgcgcattaa
     7441 agaagctatt aaaacagccc ggtctgtgga agaattggtc gagttagtga ctaccaaacg
     7501 ttacaccaag gcgcgtgtca gacgcctatt gacctatatt ttgatgcagg ctagagaaaa
     7561 tgtcttgcca gaagtcattc atgtccttgg atttactgaa aaaggtaggc aacatctcaa
     7621 gtctttgaaa ggtcaggtca gtctagtcag ccgaattggc aaagaacctt gggatgctat
     7681 gacccaaaaa gcagaccaga tttaccaact aggaaatcca agtatagcag accagaattt
     7741 tggaagagtg ccgattagaa tagaaacaaa ctaagtctac cgaaaggtag gctttttgag
     7801 attttttacg aataatactc ttcgaaaatc aaattcaaat cgcgtcaacg tcgccttgcc
     7861 gtactcaagt acagcctgcg gctagtttcc tagtttgctc tttgattttc attgagtata
     7921 aatagataga aaagaaaatc ttgtacaagt tcctgacaat ttctttcttt tcgtgtataa
     7981 tagtggaaaa gagctaaaac gaggtatggt tatggaagca gtcctttact caacattccg
     8041 aaatcattta aaggactaca tgaagaaggt aaatgatgaa tttgagcctt tgacggtggt
     8101 caataaaaat ccagatgagg acattgtagt cctttcaaag agtgagtggg atagtatcca
     8161 agaaaccctg agaattgctc aaaataagga actttctgat aaggttttgc gaggaatggc
     8221 tcaagttcgt gctggaagta ctcaggtcca tgttattgag gagtaagaaa tgctgctcaa
     8281 gtttacagaa gatgcctggg cagattattg ctactggcaa aatcaggata agaaaacgtt
     8341 aaaaagaatc aataaactaa tcaaggatat tcaacgtgat ccctttacag gaataggtaa
     8401 accagaacca ctcaaatatg attaccaagg agcctggtca cggcgtattg atgcagaaaa
     8461 tcgcttgatt tatatgatgg atggagatag cgtggctttc ttgtccttta aagatcatta
     8521 ctaagtctat tgaaaggtag gctttttctc aagtctctac taaactacta tttctaaaca
     8581 acaagtttat ttttgcaaat gtgttccaaa ttccccaaaa catattctat tttaggtttg
     8641 tgaaaatcac ttttttatgg tagaatgtaa aagaatgtat gtcattcgga ataacaataa
     8701 aatgaggtaa aaacatggaa atcatgtcgc ttgcgattgc tgtttttgcc gtcatcattg
     8761 gtttagtcat tggatatgtc agcatctcag ctaagatgaa atcatctcag gaagctgcag
     8821 agttgatgct tttaaatgct gaacaagaag caactaattt acgtggacaa gctgagcgtg
     8881 aagcggattt acttgttaat gaagccaaac gtgaaagcaa gtctcttaaa aaagaagcac
     8941 tattggaggc caaagaagaa gccagaaaat accgtgaaga agtggacgct gaattcaaat
     9001 cagaacgtca agaactcaaa caaatcgaaa gtcgtttgac agagagagct actagccttg
     9061 accgtaagga cgacaatttg acgagtaaag aacaaacact tgaacaaaaa gaacaaagta
     9121 tttctgatag agcgaaaaac cttgatgcgc gtgaagagca attagaggaa gtcgaaagac
     9181 aaaaagaagc agaactagag cgtattggtg ctctgtctca ggcagaagca cgagatatta
     9241 tcttggctca gacagaggaa aacttgacca gggagattgc cagtcgcatt cgcgaagctg
     9301 agcaagaggt caaggaacgt tctgacaaaa tggccaagga catcttggtt caagctatgc
     9361 aacgtatcgc tggtgaatat gtagcggagt caacaaactc aacggttcat ctgccagacg
     9421 atactatgaa gggacgcatt attggtcgtg aaggtcgtaa cattcgtacc tttgaaagtt
     9481 tgacaggggt cgatgtgatt atcgacgata caccagaagt ggtgaccttg tcaggatttg
     9541 atccgattcg tcgtgagatt gcccgtatga ctatggaaat gttgctcaaa gatggtcgta
     9601 tacatccagc tcgtatcgaa gagttggttg agaaaaaccg tcaagagatt gacaataaga
     9661 ttcgtgaata cggtgaggct gctgcctatg aaattggtgc gccaaacctt catccagact
     9721 tgatgaagat tatgggacgt ttgcagttcc gtacttcata tggacaaaat gttttgcgcc
     9781 attcgattga ggttgctaag ttggctggta tcatggcgag cgaacttggt gaaaatgcgg
     9841 ctcttgcccg tcgtgctgga ttccttcacg atatcgggaa agccattgac catgaggttg
     9901 aaggtagcca cgttgaaatc ggtatggaat tggcccgtaa gtacaaggaa cccccagttg
     9961 tggtgaatac gattgctagt caccacggag atgttgaagc tgagagcgtg atagcagtta
    10021 tcgtcgctgc agcagatgcc ttgagcgcag cccgtccagg tgctcgtagt gagtctcttg
    10081 aaagctacat caagcgtctc catgatttgg aagaaattgc taacggcttt gaaggagtgc
    10141 aaactagctt tgcccttcaa gcaggacgtg aaattcgtat catggtcaat ccaggaaaaa
    10201 tcaaggacga caaagtcaca atcttggctc acaaagttcg taagaaaatt gaaaacaatc
    10261 tcgattatcc aggaaatatc aaggtaaccg tgattcgcga gcttcgtgca gtagattatg
    10321 ctaaataaat aagaaagagc agttgaaaaa ttactgcttt tttgttacac tagatagaaa
    10381 gactgtaaag gataatctgg ctttttgcca ttattctaga gaaatgttat ttcacagatt
    10441 gactaaaagg agacacaatg gcagaccgag gcttactaat cgttttttct ggtccttcag
    10501 gggttggaaa aggaacggtt agaagagaga tttttgagag ttctgaaaac caatttcaat
    10561 actctgtatc gatgacgaca cgcgcacaac gtcctggaga agtggacggt gtcgactatt
    10621 tcttccgtac tcgtgaagaa tttgaagagc tgattcgtca aggacagatg ttggaatacg
    10681 cagaatatgt cggtaactac tatggaactc ctctgaccta tgtcaatgaa accttggaca
    10741 agggaatcga tgttttcctt gaaattgaag ttcagggtgc tcttcaggtc aagaaaaagg
    10801 ttccagatgc tgtctttatc ttcctgacac caccagattt ggatgaattg caagatcgct
    10861 tggtaggtcg tggaacagat agtgcagaag tgattgccca acgaatcgaa aaggccaagg
    10921 aagaaattgc cctcatgcgt gagtatgatt atgccattgt caacgatcag gtgcccctag
    10981 ctgctgaacg tgtcaaatgt gtaattgaag cagaacactt ctgtgtggat cgtgtcattg
    11041 gtcactatca ggagatgtta ccaaaatctc caactacccg ataaaattta gaaaataggt
    11101 acaagcaaat gatgttaaaa ccctctattg ataccttgct cgacaaggtt ccttcaaaat
    11161 attcactcgt aatcttggaa gcaaaacgtg cccacgaatt ggaagcaggt gccccagcaa
    11221 ctcaaggttt caagtctgaa aaatcaactc ttcgcgcttt agaagaaatc gaatcaggaa
    11281 acgttacaat tcacccagat ccagaaggaa aacgcgaagc agtgcgtcgc cgtatcgaag
    11341 aagaaaaacg ccgcaaagaa gaagaagaaa agaaaatcaa agagcaaatt gctaaagaaa
    11401 aagaagatgg tgaaaaaatt taaggttggg gggactcaat cttatttttt ctattgcaag
    11461 aatgtactga caaggaggag gtgagaagat ggccctagct aagattatcg tagatgtgcc
    11521 cttgatgcag acggaccagc cctatagtta caggattccg gaggaatttg agggaatgct
    11581 ggaagttggg atgcgggttc atgtgccttt tggtaagggt aatcgcctga ttcaagggat
    11641 tgttcttggt ttggagtccc aatcagatgg agaagagatg gagcaagatt taaaagatat
    11701 tgcagaggtg ctggattttt ctcctgttct cacgccagaa caactctggc tggctgagga
    11761 gttacgcaaa tctgtcttct cctacaaaat ttctatcctc aaggccatgc ttccaggatt
    11821 tctgaattct agctatgaca agattctcta tcctctggaa ggcttgagtc aggaagaacg
    11881 agtgcgcctg tttggttcag aagattcgct agccttttct tcactagacc ttgccaagca
    11941 agctgaaatg atgcgtttga ctagaaaagg cctgcttggt ctggaatatc aggcagtcga
    12001 tcaaaagaag gtcaagaccc agtcttggta tgaggttgac catgctcaat tagaaggtgt
    12061 tgagatttct acacgtgcca agaaaaagtt ggaactgaga gattatctgc tgtctcatcc
    12121 agagagcgct tccttggcta gcttgttaga gtcctactcg cgagagcaag tcaacttctt
    12181 tgtggatcaa ggtgctgtta ccatagtcca aaaggaagtt caacgctcgg ctgcttattt
    12241 tgaaggaatt gaagcaagta gacctttgga gttaaatcca gaacaaagac aggcgcgtga
    12301 tgcggttgtg agttctattg gcagttctca acctcccttt ctacttcaag ggattacagg
    12361 aagtgggaag accgaggttt acttgcagat tatccaaggt gccctggata agggcaagac
    12421 agctattttg ctggtacctg agatttcctt gactcctcag atgaccgagc gttttatagc
    12481 acgttttggg gacaaggtag ccattcttca ctcaggatta tccaatggtg aaaagtatga
    12541 tgaatggcgc aaggtggagc gtggcgatgc ccaagttgtt gttggtgcca gatctgctat
    12601 ctttgctcct ctgaaaaatc tgggtgtcat gattattgat gaagagcatg aagcggctta
    12661 taagcaggac agcaatcccc gttaccatgc cagagaggta gctattttac gggctcagta
    12721 taatcaagca actctggtac ttggatctgc aacgccgagc ttagagagcc gggcacgtgc
    12781 tggcaaaggc gtctatcaac acttacgtct aacccaacgt gccaatcctt tggctacaat
    12841 ccctgaggtt caagtgattg actttcgaga ctatatcgga caaaatgaga cgtcaaactt
    12901 tacgcctcct ttgctagagg ctatccaaga ccgtctggtt aaaaaagagc aggtggttct
    12961 catgctcaat cgccgtggtt attctagctt tgtcatgtgt cgggagtgtg ggacggtgga
    13021 tacttgtccc aactgcgata tttctctgac cttgcatatg gataccaaga ctatgaactg
    13081 ccattattgt ggtttctcga aggatattcc tcaggtctgt cctaactgta agagccacag
    13141 tattcgttac tatgggacgg gaactcagaa ggcttatgat gagctagcag aactctttcc
    13201 ccaagttcgc attttgagga tggatgtgga tacgactcga aagaaaggca gtcaccaagc
    13261 cttgcttgac cagtttgggc gaggggaagc ggatatttta cttggtactc agatgattgc
    13321 aaagggtttg gattttccca atgttaccct agtaggagtt ctaaatgcgg atacggcctt
    13381 gaatctgcct gatttccgtt cttctgagag aaccttccag ctcttgactc aggtggcagg
    13441 tcgagcaggt cgggctgaaa aagcaggtca agtcttgata cagtcctaca attcgcagca
    13501 ctatgctatt cgatttgcca aggatcaaga ttacgaaggc ttttatgcct atgaaatggg
    13561 aatcagacga caactcggct atccacctta ctatttcacg attggtatta ccctttctca
    13621 caagaaagaa gaagaggttt tcaaacgtgc ctatgaagtc atgaacattt tgcggtcagg
    13681 cttgtcagag accagtccca ttctggggcc aacgccaaaa ccgattgccc gtacccgcaa
    13741 cctctatcat taccagattt taattaaata ccgtttagaa gatgagctgg ggccgaccct
    13801 caaccaggtc ttggccttga ctcaagaacg ggaaaatagt gagctccgtc tcagcattga
    13861 ccatgagccg cagcaatttt tataagaagg agaagatatg acaaaactaa tctttatggg
    13921 gacccccgac ttttcagcaa cagtcttaaa aggacttttg acagatgacc gttacgaaat
    13981 tctagccgtt gtgacccagc caaaccgtgc tgttggtcgt aaaaaagtta tccaagaaac
    14041 cccagtcaag caggctgcca aggaagcagg actatctatc taccaacctg aaaaattatc
    14101 tggaagtcca gagatggaag atcttatgaa gctaggagca gatggaattg tgactgctgc
    14161 ttttgggcag tttctcccaa gcaaactcct tgatagcatg gactttgctg tcaacgttca
    14221 tgcctccctc cttcctagac accgtggtgg tgcgcctatc cattatgcct tgattcaagg
    14281 ggatgaggaa gctggtgtga ccatcatgga aatggttaag gaaatggatg caggagatat
    14341 gatttctcgt cgcagcattc cgatcacaga tgaggacaat gttggcacct tgtttgaaaa
    14401 attggcgcta gttggtcgtg atttgctttt ggacactctg cctgcctata ttgctggtga
    14461 tatcaaacct gaaccgcagg atacgagtca ggttaccttc tctccaaata taaagccaga
    14521 ggaagaaaaa ctggactgga acaaaaccaa tcgtcaactc tttaaccaaa ttcgtggaat
    14581 gaacccctgg cctgttgccc atactttcct taagggcgac cgctttaaga tttatgaagc
    14641 cctaccagta gaaggtcagg gaaatccagg tgagattctc tctatctgca agaaagaatt
    14701 gattgtcgca acggctgaag gggctctatc cctcaaacaa gtgcagccag ctggtaagcc
    14761 taagatggac attgcttcct tcctcaacgg agttggacgt acattgactg taggagaacg
    14821 atttggtgac taaagtagaa acggctagaa gtttagctct agcagtgcta gaggatgttt
    14881 ttgtgaatca agcatattca aatattgcct taaataaaca cctcaagggg agtcagctct
    14941 ctgcagcaga caagggctta gtgaccgagc tagtctatgg aacggtagcc cttaaactga
    15001 ctctggaatg gtacctatcc cactttatcg aagacagaga ccagttagac agctggctct
    15061 atgtccttct tctcatgagt gcctaccaac tccgctattt ggacaagatt ccagatcatg
    15121 ctgtggtcaa tgaagcagtg gaattggcca aactccgtaa aaaaggcagt gaaaaattgg
    15181 tcaacgctgt ccttcgccgt atcttgcgtg aaggctggcc agatattgct agcatcaagc
    15241 gaaaaaacaa gcgtgactcc attgcctatt ctctcccagt ttggctagtt gccaaactca
    15301 aggaagaata cggagaggag agagcaaaag ccatctttga aagcctgtta gtgcgaaaca
    15361 aagccagtat tcgtgtaaca gacctaagtc gaaaagagga aatccaagcc ttgttggagg
    15421 cgaataattc ccttttagca accactggtc tggttaagga gcaggggcat tttgcagggc
    15481 ataatttgtt tgcgaatgga gccattacca tccaagatga gtccagtcag ctggttgctc
    15541 cgacgcttga tttacaaggt gatgagcgag tgcttgatgc ctgtgcggct ccaggtggga
    15601 aaacagccca tatagcctct tatcttacga caggtcaggt tactgctctg gacttgtacg
    15661 accacaagtt ggatttaatt caagaaaatg cccaacgtct gggagttgca gatcgggttc
    15721 aaactcaaaa attggatgcc agaaaggtgc atgagttttt tgaccaggat tcctttgata
    15781 agattttggt ggatgctcct tgttcaggaa tcggtctttt gcgccgaaaa ccagatatca
    15841 aatacaataa agaaacggca gatttcgcgt ccttgcagga aattcagtta gaaatattag
    15901 gtagtgtttg tcaaatacta ggcaaaggtg gtataataac ttatagcacc tgcactatcg
    15961 tctcagagga gaattttcaa gtcgttaagg cctttttaga gagtcatccc gagttcgagc
    16021 aggtaaaact agaacatgaa tgtaaggata tcatgaaaga cggctgtatc ctcattacac
    16081 ctgaattgta tggaagtgat ggattcttca tcagtcaatt tcgcaagata tcggattagg
    16141 aaggaactga cacatggaaa tttcattatt aacagatgtt ggtcagaaac gaacaaataa
    16201 ccaagactat gtcaaccact atgtcaatag agctggacgt accatgatta ttttagctga
    16261 tgggatggga ggtcatcgcg cagggaatat cgctagtgaa atggcggtca cagacctggg
    16321 tgtagcttgg gttgataccc agatcgatac agtcaatgaa gtgcgtgaat ggttcgccca
    16381 ttacctagaa attgaaaatc aaaagattca ccagcttggt caggatgaag cttacagagg
    16441 catgggaact actttggaag tccttgctat tattgataat caggctatct atgctcatat
    16501 tggtgattcg cgtatcggct tgattcgtgg agaagaatac catcagttga cgagtgatca
    16561 ttctttggtt aatgaattgc tcaaggctgg tcaattgaca ccagaagagg cagaagctca
    16621 tccgcaaaaa aatattatca cccagtctat tgggcaaaaa gatgaaattc agcctgattt
    16681 tgggacagtt atccttgagt caggtgacta tctcttgctc aatagtgacg gcttgaccaa
    16741 catgatttca ggcagtgaga ttcgtgatat tgtaaccagt gatattcctt tagcagataa
    16801 aacggagaca cttgttcgtt ttgctaacaa tgcaggaggt ttagacaaca ttacggttgc
    16861 ccttgtttct atgaacgagg aggatgaaga atgatccaaa tcggcaagat ttttgccgga
    16921 cgctatcgga ttgtcaaaca gattggtcga ggaggcatgg cggatgtcta cctagccaaa
    16981 gacttaatct tagatgggga agaagtggca gtgaaggttc tgaggaccaa ctaccagacg
    17041 gacccgatag ctgtagctcg ttttcagcgt gaagcgagag ctatggcaga tctagaccat
    17101 cctcatatcg ttcggataac agatattggt gaggaagacg gtcaacagta tcttgcaatg
    17161 gagtatgttg ctggactaga cctcaaacgc tatatcaagg aacattatcc tctttctaat
    17221 gaagaagcag tccgtatcat gggacaaatt ctcttggcta tgcgcttggc ccatactcga
    17281 ggaattgttc acagggactt gaaacctcaa aatatccttt tgacaccaga tgggacggcc
    17341 aaggtcacag actttgggat tgctgtagcc tttgcagaga caagtctgac ccagactaac
    17401 tcgatgttgg gctcagttca ttacttgtca ccagagcagg cgcgtggttc gaaggcgact
    17461 gtgcagagtg atatctatgc catggggatt attttctatg agatgttgac aggccatatc
    17521 ccttatgacg gggatagcgc ggtgaccatt gccctccagc atttccagaa acccctgccg
    17581 tccgttattg cagaaaatcc atctgtacct caggctttag aaaatgttat tatcaaggca
    17641 actgctaaaa agttgaccaa tcgctaccgc tcggtttcag agatgtatgt ggacttgtct
    17701 agtagcttgt cctacaatcg tagaaatgaa agtaagttaa tctttgatga aacgagcaag
    17761 gcagatacca agaccttgcc gaaggtttct cagagtacct tgacatctat tcctaaggtt
    17821 caagcgcaga cagaacacaa atcaatcaaa aacccaagcc aggctgtgac agaggaaact
    17881 taccaaccac aagcaccgaa aaaacataga tttaagatgc gttacctgat tttgttggcc
    17941 agccttgtat tggtggcagc ttctcttatt tggatactat ccagaactcc tgcaaccatt
    18001 gccattccag atgtggcagg tcagacagtt gcagaggcca aggcaacgct caaaaaagcc
    18061 aattttgaga ttggtgagga gaagacagag gctagtgaaa aggtggaaga agggcggatt
    18121 atccgtacag atcctggcgc tggaactggt cgaaaagaag gaacgaaaat caatttggtt
    18181 gtctcatcag gcaaacaatc cttccaaatt agtaattatg tcggccggaa atcttctgat
    18241 gttatcgcgg aattaaaaga gaaaaaagtt ccagataatt tgattaaaat tgaggaagaa
    18301 gagtcgaatg agagtgaggc tggaacggtc ctgaagcaaa gtctaccaga aggtacgacc
    18361 tatgacttga gcaaggcaac tcaaattgtt ttgacagtag ctaaaaaagc tacgacgatt
    18421 caattaggga actatattgg acggaactct acagaagtaa tctcagaact caagcagaag
    18481 aaggttcctg agaatttgat taagatagag gaagaagagt ccagcgaaag cgaaccagga
    18541 acgattatga aacaaagtcc aggtgccgga acgacttatg atgtgagtaa acctactcaa
    18601 attgtcttga cagtagctaa aaaagttaca agtgttgcca tgccgagtta cattggttcc
    18661 agcttggagt ttactaagaa caatttgatt caaattgttg ggattaagga agctaatata
    18721 gaagttgtag aagtgacgac agcgcctgca ggtagtgtag aaggcatggt tgttgaacaa
    18781 agtcctagag caggtgaaaa ggtagaccta aataagacta gagtcaagat ttcaatctac
    18841 aaacctaaaa caacttcagc tactccttaa aagcagatgg ataatcaaaa tgagtaatca
    18901 atctttgtca tgatttcatg gcaaagattt tttttgagtc cagatttgtg atagaataga
    18961 gggagttgat aaaaggagga aagcatggaa gaatcaaaag aattaaatgc cgtcattgat
    19021 gtgattatgc tagcggggac tattctcctt aaaagtggct cagaaatcca tcgtgtagaa
    19081 gataccatga ttcgaatcgc gcattcgcag gggattgtgg attgcaatgt ccttgccatg
    19141 cctgccgcta tctttttctc tattgaaaat accaatattt cgcgcatgaa gcgcgtgacc
    19201 tcctcttctt ataacatcga aaaagtctgc gatgtgaacc agatttctcg tcagctagtt
    19261 ggggggcaga ttgacttaga aacagccttt aagcaattga cggccttgca agctcaaccc
    19321 cttccctata ctaagttgca ggtaactctg gctgcgacct ttagtgctcc tttcttttca
    19381 gttatgttta gcggaaatat ctacgacgca cttggtgcag gagtggcgac cttatttggt
    19441 tttgcctttt ccctctatgt ggaaaaattt atccgaattc cctttgtgac agcctttgct
    19501 ggagcctttg tctttgggat aattgcccag ttttgggctc gctacacagg ttttccttca
    19561 acggcagatt tgattatagc tggtgcggtc atgccgtttg taccaggtat tgccttgacc
    19621 aatgcggtcc gtgatattat gaccaaccac ataaactctg gtatgagtaa gatgtttgaa
    19681 tccctgctca ttaccctcgc tttaggggca ggaacttctg tcgccttggt attgatgaac
    19741 taatatgaca ctaacaacct ttttattaca agcagtagca agttttcttg ccattatcac
    19801 ttttttaatc gtactcaatg tgcaacggtc tatgctctta cctggaggga ttttgggcat
    19861 gactgtctgg ctaatctatc tcttgctcaa ggaaccgacc aatgtcattg tagctacctt
    19921 cattgcagcc attattggtt cttgtgtcag ccagatttta agtattcttt ataagacacc
    19981 tgctgtggtc tttatcttgg ccattttggc accgctggtt ccaggttatc tctcctaccg
    20041 aacaactgcc ttttttgtga caggggacta taataaagca ctggcaagtg cgaccttggt
    20101 tgtcatgttg gctttggtaa tctctattgg aatggctagc ggaacagtga ttctcagact
    20161 gtatcattat ataaaaacac atcgagtatc gtagacttta cagaaataaa agaattttct
    20221 gaaaaatgag ataaataaat taacaacgct ttctatatgt gcgagaatac cgcacttatg
    20281 aagaaattgc gactgatttt ggtatccacg aaagcaactt actccgtcgg agccaatggg
    20341 ttgaagtaac tcttgttcaa aggtggtgtt acgatttcaa gaactcctct cagttctgaa
    20401 gacacggtaa tgattgatgc gacggaagta aaaatcaatc gccctaaaaa aaacaattag
    20461 cgaatgattc tggtaaaaag aaatttcacg ctatgaaggc tcaagcgatt gtcacaagtc
    20521 aagggagaat tgtttctttg gatatcactg tgaactattg tcatgatatg aagttgttca
    20581 aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt ggctgacagt ggttatcaag
    20641 ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa ctcaagctgc
    20701 taacagttga agataaagcc tataaccatg cgctatctaa ggagagaagc aaggttgaga
    20761 acatctttgc caaagtaaaa acgtttaaaa tgttttcaac aacctatcga aatcatcgta
    20821 aacgcttcgg attacgaatg aatttgattg ctggcattat caatcatgaa ctaggattct
    20881 agttttgcag gaagtctact actttcctta ttgtctgtaa atctactgac cttgttgttt
    20941 atcccagtca tggtttctag ttcgggctca gagtttcaaa gtggatggca agagcatcaa
    21001 ttgattgctg agaaggttag taaaacactt gataagacat ttgataagga tgtcagagaa
    21061 attccgacca gtcagtttta tcaaaaattt gtagatgaga tgggaaggac ttactcagga
    21121 aatttgatcc tccaggagct gataactgtg aatggagctt ataaagctac ttatatcggt
    21181 gaactctcta gcaactaatc attctttaaa atgatgacca ataagaggct tgatttggtg
    21241 aatcaagtct tttttctctc ttttactgcc atttgtgata aaatagtata gaacgatttt
    21301 ttacatgaat gataaaacag aggtaaatat gacaatcggt attgataaga ttggttttgc
    21361 gaccagtcaa tatgtcttga aattacaaga cttagcagaa gcgaggggaa ttgaccctga
    21421 aaaattaagt aaaggactct tactcaagga attgagtatt gcgcccctaa ctgaggatat
    21481 cgtgaccttg gcggccagtg ctagtgactc tattttaact gagcaagaaa gacaagaagt
    21541 tgacatggtc attgtggcga ccgagtcagg aattgaccag agtaaggctg cggccgtctt
    21601 tgtgcatggc ttgctgggca tccagccctt tgctcgtagt ttcgagatta aagaagcctg
    21661 ctatggggcg actgctgccc tccattatgc caaattgcat gtggaaaatt ctccggagtc
    21721 caaggtcttg gtcattgcaa gtgatattgc caaatacggt attgaaactc cgggagaacc
    21781 cactcagggt gccggaagtg tggctatgtt gattacacaa aatccacgca tgatggcctt
    21841 taataatgac aatgtagctc agacccgtga catcatggat ttctggcgac caaattactc
    21901 gacaactcct tatgtaaatg gtgtctattc tacccaacaa tacttggata gtttgaaaac
    21961 gacttggctt gaatatcaaa aacgctacca gcttactttg gatgattttg cggctgtttg
    22021 tttccacttg ccttatccta aattagcgct aaaaggcttg aaaaaaatca tggataagag
    22081 cctgcctcaa gagaaaaaag acctcttaca aaagcatttt gaccagtcta ttctctacag
    22141 tcaaaaggtg gggaatatct acacaggttc acttttcctt ggacttttgt ctctcttgga
    22201 aaatacagat agcttgaaag ctggggataa aatcgccctt tatagttacg gaagtggagc
    22261 tgtggctgag ttcttcagtg gtgaattggt tgaaggatat gaagcttatt tggataaaga
    22321 ccgcttgaac aagctcaacc aacgaactgt cttatccgtt gcagactatg aaaaggtctt
    22381 ttttgaggaa gtaaacttgg atgaaacaaa ctctgcccag tttgctggct atgaaaatca
    22441 agattttgcc ttggttgaaa ttctcgacca ccaacgccgt tatagcaagg ttgaaaaata
    22501 atgaagataa gttggaatgg gttttctaaa aaatcatacc aagagcgcct cgagctgtta
    22561 aaagctcagg cgctccttag tcctgagaga caagctagtc tggagaagga tgaacagatg
    22621 agtgtgactg tggcagacca gctgagtgag aatgtagtgg gaactttttc tctgccttat
    22681 tcgctggttc cggaggtact tgtcaacggt caggaataca ccgttcccta tgtgacagaa
    22741 gaaccctctg tggttgcggc ggccagctat gccagcaaaa tcatcaagcg tgcaggtggt
    22801 tttactgcac aagtccatca gcgccagatg attgggcagg tagcccttta tcaagttgct
    22861 aatcctaaac tagcgcaaga gaagattgcc agcaagaaag cggagctctt ggagcttgcc
    22921 aatcaagcct atccttctat cgttaaacgt gggggtgggg cgcgtgacct gcatgtcgag
    22981 cagataaaag gcgaaccaga ctttctcgtt gtttatattc atgtcgatac ccaggaagcc
    23041 atgggtgcca atatgctcaa caccatgctg gaagccttga aaccagtctt agaagaactc
    23101 agtcagggac agagtctcat gggaatcctg tccaactacg cgaccgattc tctggtgact
    23161 gcaagctgtc gcatggcctt tcgctacttg agccgccaaa aggatcaagg acgagagatt
    23221 gcggagaaaa ttgcgttggc tagtcagttt gcgcaggatg atccttaccg agctgctact
    23281 cataataaag gaatttttaa tggtattgat gcgattttga ttgccactgg taatgactgg
    23341 cgtgccatcg aagctggggc ccatgccttt gccagtcgag atggacgcta tcaaggtctt
    23401 agttgctgga cgctggacct tgaaagagaa gaattggtcg gtgagatgac cctgcccatg
    23461 cctgtagcga ctaagggtgg ctctatcggc ctcaacccac gtgtagctct cagtcatgat
    23521 ctactaggaa atccttctgc cagagaatta gcccagatta tcgtgtctat cggtcttgcc
    23581 caaaattttg cagccctcaa agccttggta agtacgggca tccagcaagg ccacatgaaa
    23641 ctacaggcca aatccctagc tctcctagct ggggctagtg aatctgaagt tgctccccta
    23701 gtcgagcagc ttatcgcaga taaaaccttt aacctagaga cagcccagcg ctatctcgaa
    23761 aatttaagat cataaaaaac tcagacgaat cggtctgagt tttcttgtgt ttaaatactt
    23821 tttcctggta atagtgtaac tggtaagaag taaccagttg tggtgacttc cttgccttca
    23881 atcttttgca agagaagatc aatagtgaga caagcaatct cttccaaagg ttgcttgata
    23941 gtagccaatt gagggtagta attttcgata aagtaggtcc catcatagcc gatgaccttg
    24001 agctcttttg ggacagaaat gcccaattct tgagcgattt taatgaccag aatagctgtc
    24061 aaatcatccg aagcaaaaat ggcatctggt ttttcccggg tcaagatatt cttgatttcc
    24121 atttcttttc tgacgggaga aaagtcactg gaaacattga taataggagc ttttgggagt
    24181 acggatgcaa aaccagcgtg gcgcagtccg gttggcgaat tggaattgtc attccctgta
    24241 atcatgatga tagactgggc acctgtcttg accaaggttt gggcagcaag aaccccacca
    24301 gcatagttgt cagaggagac gacagggatg tctggcgata ggtttcggtc aaaggaaata
    24361 atcggcgctg tcacacgatt gtagtcttcg attcctaggt tgtgactacc agaaatgatg
    24421 ccgtccacct gattggcttc caacatttcg atgtattcac gttccttctc agaatcatgt
    24481 tcactgttgc agatgatggt cttgtaacca tttttgaaga gttggtgttc caatttatca
    24541 atcaattctg catagaaaac attggaaata ttggggaaaa tcaagccgat taacttagct
    24601 gattttcctt gcagactacg agccaggttg ttgggtttat agcccaattc tcgcatggct
    24661 tcattgactt tttggatggt tttctcagat agataccctt ttttattgat aacccgagaa
    24721 acggtagtag gactgacgcc tgcaagtttg gcgacatcag ttagttttgc gaccataatc
    24781 taattcatag taagttccag ttgggtttcc agatttgatc aggataccat tttggtccgc
    24841 atgtgggaag acacgaccag aaaatacttt ttctccttta ttgatgaaaa tttcaaagac
    24901 agagttatcg atgaagattg tagcagtagt agcctgattc tcgatagggc aagaacgagt
    24961 tgtcccaaat tcttgggcat actgttctcc agcctggcta cgatccactg tcacttgacc
    25021 gtttacaagg tcaaagttga ttgaaagtcc cttaccttct ttatcagcaa gtaagacaat
    25081 ctcgctctgg ctattagctt ccaagttgag ttcaagttcg taagtgttct tggtttggga
    25141 acggtttgag aaggcttctt cagaagcacg aaggtcctta atagcagcga ctggatactg
    25201 gtagagcttg tcgtctttga tagtgagttc cttgaccaaa gagaaggttc cttggtggtc
    25261 aaaacggtca gatgggtaag aaacatctgg caaaccaagc cagctaactg ctagagcacg
    25321 cccatcagga gcgttgaagg cttgagttgc ataggcttcg aaaccgtaat ccatgttttg
    25381 aagttgagac acatctacca ttttggcatt tttagggtca aaggaagccc caatcttata
    25441 catatttgga aagatattat cgtagtctag aactttctta tccaatcctt gtggacagta
    25501 gagaaggaca ggttgttcct ctacaaagac cagattagga cattccatca tgtaggcagt
    25561 acggtcgtta gcaaagtcaa ggtcgccaac tgcttgccag tttgtgtagt cgttattgac
    25621 agccttgtag agacgaacga aacctttttt ctccaagtcc tgtccaccga cgacagcata
    25681 gtattgacct ttaaagttaa aaatttgtgg atcgcggaag tggtcagtag agtctgctgg
    25741 ctggtcaatc aagatcttgt caatctttgt aatcttaccc tccttgtcca tcaaagcacc
    25801 gatctggtat gggtgacgga tccagttttc atcgcgaaca tttcctgtat aaaataggaa
    25861 taagttatcg ccaaattgca tggcagaacc agagtaggca ccgtggctat ctaatggagt
    25921 atctggtaaa actttgattc cagtttcttt aaagtgaacc aaatcatcac tttctagctg
    25981 tgcccaagat tttaaaccgt gggctgcacc aaaaggaaaa ttctggtaaa agaggatcca
    26041 cttgccatca aagtaagaaa agccatttgg gtcgttgaga agtcctgttt ttggctcaac
    26101 atggtaatga gtatgccatg gagattgtgc catattttcc tttatatgtt gaatttcttc
    26161 ttgcgtccaa tcttgataaa gtctgtaacg acgctcagtt gtccattcca ttcattcctc
    26221 caaaaatctt atcactttta atagtaactg ttttcgataa aaaatgcaag ccttttatgt
    26281 taaaaaagag gaaaaactgt caa
//
LOCUS       DAVLYR010000011        26161 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLYR010000011 DAVLYR010000000
VERSION     DAVLYR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.011"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(88..324)
                     /locus_tag="VYO63_000434"
     CDS             complement(88..324)
                     /locus_tag="VYO63_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1671414.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(337..810)
                     /locus_tag="VYO63_000435"
     CDS             complement(337..810)
                     /locus_tag="VYO63_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671415.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(828..1013)
                     /gene="briC"
                     /locus_tag="VYO63_000436"
     CDS             complement(828..1013)
                     /gene="briC"
                     /locus_tag="VYO63_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1671416.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(1172..1939)
                     /locus_tag="VYO63_000437"
     CDS             complement(1172..1939)
                     /locus_tag="VYO63_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1671417.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKHAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(1936..2802)
                     /locus_tag="VYO63_000438"
     CDS             complement(1936..2802)
                     /locus_tag="VYO63_000438"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1671418.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2839..4206)
                     /gene="accC"
                     /locus_tag="VYO63_000439"
     CDS             complement(2839..4206)
                     /gene="accC"
                     /locus_tag="VYO63_000439"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1671419.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4218..4640)
                     /gene="fabZ"
                     /locus_tag="VYO63_000440"
     CDS             complement(4218..4640)
                     /gene="fabZ"
                     /locus_tag="VYO63_000440"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1671420.1"
                     /translation="MIDIQEIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(4637..5122)
                     /locus_tag="VYO63_000441"
     CDS             complement(4637..5122)
                     /locus_tag="VYO63_000441"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1671421.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5125..6360)
                     /gene="fabF"
                     /locus_tag="VYO63_000442"
     CDS             complement(5125..6360)
                     /gene="fabF"
                     /locus_tag="VYO63_000442"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1671422.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6382..7113)
                     /gene="fabG"
                     /locus_tag="VYO63_000443"
     CDS             complement(6382..7113)
                     /gene="fabG"
                     /locus_tag="VYO63_000443"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1671423.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(7147..8067)
                     /gene="fabD"
                     /locus_tag="VYO63_000444"
     CDS             complement(7147..8067)
                     /gene="fabD"
                     /locus_tag="VYO63_000444"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1671424.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(8060..9034)
                     /gene="fabK"
                     /locus_tag="VYO63_000445"
     CDS             complement(8060..9034)
                     /gene="fabK"
                     /locus_tag="VYO63_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1671425.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(9153..9377)
                     /locus_tag="VYO63_000446"
     CDS             complement(9153..9377)
                     /locus_tag="VYO63_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1671426.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(9437..10411)
                     /locus_tag="VYO63_000447"
     CDS             complement(9437..10411)
                     /locus_tag="VYO63_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1671427.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10411..10845)
                     /locus_tag="VYO63_000448"
     CDS             complement(10411..10845)
                     /locus_tag="VYO63_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1671428.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(10939..11724)
                     /locus_tag="VYO63_000449"
     CDS             complement(10939..11724)
                     /locus_tag="VYO63_000449"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1671429.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12093..13457
                     /locus_tag="VYO63_000450"
     CDS             12093..13457
                     /locus_tag="VYO63_000450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1671430.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13460..13831
                     /locus_tag="VYO63_000451"
     CDS             13460..13831
                     /locus_tag="VYO63_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1671431.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            14045..15319
                     /gene="serS"
                     /locus_tag="VYO63_000452"
     CDS             14045..15319
                     /gene="serS"
                     /locus_tag="VYO63_000452"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1671432.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDASVTIPEALRPYMGGAEVIKP"
     gene            complement(15344..16243)
                     /locus_tag="VYO63_000453"
     CDS             complement(15344..16243)
                     /locus_tag="VYO63_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1671433.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16353..16901)
                     /locus_tag="VYO63_000454"
     CDS             complement(16353..16901)
                     /locus_tag="VYO63_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1671434.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17119..18441)
                     /locus_tag="VYO63_000455"
     CDS             complement(17119..18441)
                     /locus_tag="VYO63_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1671435.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18532..18620)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18784..21120)
                     /locus_tag="VYO63_000456"
     CDS             complement(18784..21120)
                     /locus_tag="VYO63_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1671436.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRKVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21224..21772)
                     /locus_tag="VYO63_000457"
     CDS             complement(21224..21772)
                     /locus_tag="VYO63_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1671437.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQIYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(21769..22071)
                     /gene="zapA"
                     /locus_tag="VYO63_000458"
     CDS             complement(21769..22071)
                     /gene="zapA"
                     /locus_tag="VYO63_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1671438.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22158..23042
                     /locus_tag="VYO63_000459"
     CDS             22158..23042
                     /locus_tag="VYO63_000459"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1671439.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISNPISLEE
                     KAEGKYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQG
                     LNFCAKLHFKNTEKAKNA"
     gene            23054..23668
                     /gene="lepB"
                     /locus_tag="VYO63_000460"
     CDS             23054..23668
                     /gene="lepB"
                     /locus_tag="VYO63_000460"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1671440.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            23796..>26161
                     /locus_tag="VYO63_000461"
     CDS             23796..>26161
                     /locus_tag="VYO63_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1671441.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
BASE COUNT         7670 a         6051 c         5075 g         7365 t
ORIGIN      
        1 ggatttaccc actacaaata ttatagagcc aacaataaaa agaaaaagtg tttgatagat
       61 atcaaacact tttttctttg cctcccacta tctaaaaaaa tgataataga tataattgta
      121 aacaaaaatc cagataggtt ttgcatgatt gagaaagtta aaaaaactat ggcagagaat
      181 cgttaatctc agattgtcgg tagaacgata aacaagggca aaaaagaaac caatcagact
      241 ataatataat aaactaattg gatctctgtg agatagtatc aaatggctaa tcccaaagat
      301 gatagcagat aggataacat ccaaatagta cttggactag ggaaagaagg tattcataaa
      361 ataccctcta tcaagagtct cctcaaaaac aggaccgatg attacaggca ggacaaaaga
      421 taagatagtc gataaaaagg ttggttgtcc atttgaaaaa agcacggtaa aatactcatc
      481 atgaatattc ctatgattaa tcaaatgagc atagcgtgcc caaaaattac cgagaatctg
      541 ataaaccaca taagttgcaa ataagtagaa gacaaatgac cagttccagc tctttttctc
      601 aaagataaag agcatctttt tcttttttaa cctccaaatt aatagaagga aacttcccac
      661 taatcccatt gttaaaataa gagaatagac atcagctcct aaccctaaaa tgatcgtcac
      721 atacaatcca attgtttgtg gtaaataggt agatagtaaa ataataagca aaaatattcc
      781 aaattgtctt agtttttttg tgtttctcat cgtacttttt tgaaagatta ccctgctcgg
      841 aagccgtact tccaagcatc tatataagaa ttaagtgccc cttgcctcat atagggagca
      901 aattctctat aatataacca tctactatat ccatcttccc aaacagcaag accacctgaa
      961 gtttgctcca agtcctcagt tgaaagaact gtaaatgtat ttgtacctgt cattgcaagt
     1021 accttcttaa aatagattgt tgtaggctca catttatagt atatttcttt ttttgtctat
     1081 tttatagccc atctcctcaa ctggcaattt ttcgacctga attacatttt tccataaaaa
     1141 atgagacctt tctagtctca tttagtcatt cttagtattt tctaaatcgt tgatagcgtt
     1201 cttccagcaa ctcttctagc ggtttttgtg aaagtctagc cagctccgtt tggagttctt
     1261 ttttgacact cttaatcagt tctttactag aaagtcctat ttcagaaatc accttatcca
     1321 ccacgtccat ttctaacagt tcatgcgaag tgattttcat cagttctgct gcttccatgg
     1381 cgcgagtacc gtccttccat aaaatggaag caaagccttc tggactgaga atggcataga
     1441 tagaattttc cagcatccag acacggtccg cgacagctag agccagagcc ccgcctgaac
     1501 caccttcacc gataataatg gcgataatag gaactttcag gtcactcatt tccatgagat
     1561 tgcgagcgat agcttcccct tgaccacgtt cttccgctcc gacaccagga taagcacctg
     1621 ctgtattgat aaaggtcaca actggacggc caaatttctc agcatgtttc atcaaccgca
     1681 gtgcctttcg gtagccttct ggatgtggtt ggccaaaatt ccgtttgagg ttgtcttgca
     1741 aactcttgcc tttttggata ccaaccactg ttacagcttg atctccaagc caaccaatac
     1801 caccaacaac tgcaccatca tcacgaaaag aacggtcacc atgtaattgg ataaattcat
     1861 caaaaatgcc tgtcgcaaag tccaaggttg tcaagcgact ctgctcacgc gcttctctga
     1921 ctatttttgc aatattcatc taggactccc tccatgcaat ctgactaggc tagcaatcgt
     1981 atctggtaag tctcttcttt tgacaatagc atccacaaag ccatgttcta ataggaattc
     2041 tgccttttgg aaatcctcag gcaagctttc acgaaccgta ttttcaatca cacgacgccc
     2101 agcaaaacca accaagctct gtggttcagc cagaatgata tcgccttcca tagcgaaaga
     2161 agctgtcaca ccaccagtcg ttggatctgt caaaatggtc aggtaaaaga gaccagcatt
     2221 tgaatgacgt ttaaccgccg cagagatctt agccatctgc atgagactca tgattccttc
     2281 ctgcatacgg gctccaccag aggctgtgaa taggacaact ggcaattttt cgacagtcgc
     2341 atactcaaac aaacgagtga ttttttcacc tacaaccgta cccatagaag ccatgataaa
     2401 gttagaatcc ataatcccga gagccacagt ctgaccttta ataagagcag ttcctgtcac
     2461 aacggcttca tgcagacctg ttttttcacg catagatgcc agtttctttt ggtaaccagg
     2521 gaaatgcaag ggatccttgc tttcaatccc tgtaaacaat tctttgaagg ttcccatatc
     2581 aatcgtcaaa gccaagcgtt cttgggcaga aatacgaaag gtatagctac agtgcggaca
     2641 gatacgttca cttcccagat ccttctgata gatggtatgc ttacagcctg gacactggga
     2701 aaataattca tctggaacct ctggcttagc ttgaggtttt tccctaaccg aacgattggg
     2761 attgattcga atatacttat cttttttact aaatagagcc attgattccc cttttcggtt
     2821 taaactctta aagtcatttt attctttttc ttgatattta ggtaagaagg tttccatcaa
     2881 gaaggaagta tcataatccc cagcaatgac attgcgatct gaaatgaggt caagctggaa
     2941 atctgcattg gtctgcactc cttcaatctc taattcatag agggcacgtt gcattttcat
     3001 caaggcgtca aaacgatttt cgccgtgtac tatgattttg gcaatcatac tatcataata
     3061 aggcggaatg gtataacctg gataaactgc tgaatccacg cgcaagccaa ctccaccact
     3121 tggcagatag agattagtaa tcttacctgg acttggagca aagttaaagg ctgggttttc
     3181 tgcattgata cgacactcga tggcatgacc gcgtaggaca atatcttctt gcttaacaga
     3241 caaaggctga cctgccgcaa tgcaaatctg ttccttaacg atatcaacac ctgaaacaaa
     3301 ctctgttact ggatgttcta cctgaacacg agtattcatc tccatgaaat agaaattgct
     3361 acttgcttca tcaagaagaa attcaatggt tcctgcattc tcatagccaa caaactctgc
     3421 cgctcgaaca gcagcagcac ctatttcatg acgcagcgtt tttccgattg caatcgaggg
     3481 actttcttcc aaaacctttt ggttattcct ttgaagagaa caatcccgct cacccaagtg
     3541 aatcacatgt ccatgctcat cacctaggat ttgaacctca atgtgccgag ctggatagat
     3601 aacccgttct atgtacatgg caccattgcc ataattggcc ttggcctcac tagaggcagt
     3661 ttcaaaggca gaaacgaggt catctggttt ttcaacctta cgaatccctt taccacctcc
     3721 acctgctgaa gccttgagca taacaggata gccaattttt tcagcaacaa tcaaagcttc
     3781 ttcagagtta tgcacttctc catctgaacc tggtataaca ggcacacctg ctttaatcat
     3841 ctgagcacgc gcattgatct tatcccccat catatccata acatgaccag atggaccgat
     3901 aaacttgata cctacttctt cacacatggt cgcaaatttg gaattttcac tgagaaatcc
     3961 aaaaccaggg tgaatagctt ctgcctcagt caagactgca gctgatagaa ctgcattaat
     4021 attgagataa gactctgttg ccttgccagg accaatacaa actgcttcat ctgccaaaag
     4081 cgtatgaaga gcttccttat cagcagttga ataaaccgct accgtcgcaa tccccaattc
     4141 acgtgccgca cggataatac gaaccgcaat ttcaccacga ttggcaatta aaatttttcg
     4201 aaacatggag aacctcctta gttcccaatt gcaaaagtaa ggataccact ggctgcaagc
     4261 ttgccatcca cttcagcctt tgcttcaacc acagctatgg tgccacgacg ttttacaaaa
     4321 gtcgctgtca taaccaattg gtcgcctggt acaacttgct tcttgaactt aaccttgtcc
     4381 ataccagcgt aaaagaccag ttttccttta ttttcaggtt ttgataactc caacacacca
     4441 gcagtttgcg ccaaggcttc cataatcaga acacctggca taactgggta ttgaggaaag
     4501 tggccgttaa agaaaggttc gttaatggtc acatttttga tagcaacaat ggtatcctcg
     4561 ctcacttcca agacacggtc cactagaagc ataggataac ggtggggaag agcttctttg
     4621 atttcttgaa tatcgatcat ttgatacgta ccaatccttt accaaactca accatttctt
     4681 cgttagagac gagaatttcc gttaccacac catccttagg agctgggatt tcattcatga
     4741 ctttcatggc ttcgataatt accaatgttt gacctttttt gacactatca ccaactgtaa
     4801 cgaaggcagg tttatctggt ccagcagcca agtaaaccac tccaacaagt ggactctcta
     4861 caagatttcc ctcagtagcc acacttgctt cagctggagc tggaacttct tctgctacag
     4921 tctctgctgg agcagatgta ggagctactg gactcggtgt tgctagaacg ggtgctggag
     4981 cgacttgagt tgcaacttca ggcacaggtc tcgcttcatt cttgctaaac tgcaactcat
     5041 ccgtcccatt tttataagaa aattctctca aacttgactg gtcaaattga gtcatcaagt
     5101 ctttaatatc gtttaaattc atacttatct attctcccaa cgtttgaaag caagaactgc
     5161 attgtggcct ccaaaaccaa aagtatttga aatagcgtat ggaatttctt tctccaagcc
     5221 ttgtccataa acgacattag cttcgatata atctgatact tcacttgtcc cagctgtcat
     5281 tggtacaaag ttatggcgca tagcttcgat ggtgacgata gcttctactg cacccgcagc
     5341 ccccagcaaa tgtcctgtaa aagacttggt tgatgataca ggtacttcct taccaagaac
     5401 agctacgata gctccacttt ctcctttttc attggcagga gttgacgttc cgtgagcatt
     5461 gacataggct acttgctctg gagaaatctc agcttcttcc aaggctagtt tgatggcctt
     5521 gatagctccc tgaccttctg gatgtggaga agtcatgtgg taggcatcac aagtatttcc
     5581 gtaaccaacc acttcagcca ggatagtagc tccacgtttt tcagcgtgtt caagactttc
     5641 taaaaccaac atccctgaac cttcacccat aacaaaccca ttgcgatcct tatcaaatgg
     5701 gatcgaagca cgagttggat cctctgtagt agagagagct gttaaggctt ggaaaccagc
     5761 gatggcaaaa ggtgtgatag aagcttctgt tcctcccacc aacatcacat cttggaaacc
     5821 aaacttaatg gagcggaagg catccccaat cgcatcattt gatgaagagc aggcagtatt
     5881 gatagattta caaacaccgt ttgcaccaaa acgcatggct acattcccag aagccatatt
     5941 tggtaaagct tttggaagag tcattggttt gacacgtttg ggtccttttt catgaaggcg
     6001 aagtacctga tcttcaattt ccttgattcc accaatacca gatgcaacga taacaccaaa
     6061 acgatcccta ttaagagcct ctacatcaag attggcatga tttacagcct cttgggctgc
     6121 atacaaggca tataaagaat agttatcaaa acggttggta tcttttttta caaagtattt
     6181 atcgaacgga aaatcttgga tttctgccgc attatgcaca tcaaagtcac tatgatcaaa
     6241 ttttgtaatg ccaccaatgc cgattttccc agttgctaaa ctattccaaa attcttctgg
     6301 tgtatttccg attggagatg ttactccata acctgttacc actactcgat ttagtttcat
     6361 tcttttcacc tctagctttc gctacatact taagccacca tcaatggcaa tcacttgacc
     6421 agttagataa tcttggcctg ctaaaaatac tgtcaaatct gcaacctgct ctgcctgccc
     6481 aaattctttc atcggaatct gagctagtgt agcttcctta atcttatctg ataagatagc
     6541 tgtcatatca gactcaatca ttcctggagc aatcacattg actcgtatat tccgactagc
     6601 gacctcgcgt gccacagact tggtaaagcc aatcaagcca gccttagaag cagcatagtt
     6661 agcttgacca atattcccca tcaaaccaac aacactagac atattaatga tagcaccttc
     6721 tctggctttc atcatcggtt tcaagactga ttgtgtcata ttaaaggcac cagtcagatt
     6781 gaccttgagc actttttcaa aatctgcttc tgtcatcttg agcataagag tatcttgggt
     6841 aatccctgca ttgttgacca aaacatctac tgaacccagt tctgcaatag cttgatcaat
     6901 catacgctta gcgtctgcaa aatctgatac atctcctgaa atgggaacca ccttgatacc
     6961 atagtttgaa aactcagcga gcaattcttc tgagattgcc ccacgactgt ttaagacaat
     7021 gttggctcct gcttgagcaa acttgtgggc gatggcaaga ccaattccac gactcgaacc
     7081 tgtaataaag atatttttat gttctagttt catttttttc ctttcaaaac ttctacttat
     7141 tttagtctat ttttctaaaa gtgctactaa actcgcttga tcttccacat gagctaagtg
     7201 agcagtttga tcaatttttt taacaaaacc tgacaagact ttccccggtc caatctcgat
     7261 aaagttgctt atgcctgctt cttgcatgac cccaatactt tcatagaaac gaacgggttc
     7321 cttgacctga cgcgtcaaga gctgagcaat gtcctctttt tgcatcacag cagcttctgt
     7381 attgccgact aggggacaag taaaatctga aaaacttacc tgagctagag tttcagctag
     7441 tttctggcta gctggctcaa ggagagcggt gtgaaaggga cctgacacct taagaggaat
     7501 caagcgtttg gcacctgctt cttgcaaaag ttcaaccgct cgatcaactg caaccacttc
     7561 tccagcaatg acgatttgtg caggtgtgtt atagttggct ggagtaacca ctccaagttc
     7621 agaagctttt tgacaggctt cttcaatgac ctctactggc gtattgagaa ctgctaccat
     7681 cttgccagag tcagcaggag ccgcttcttc catataggct ccacgcttag ctaccaaggc
     7741 aaccgcatct tcaaaatcca aggcgccgct tgccaccaag gcagagtatt ctccaagaga
     7801 caaaccagca accatatcag gctgatagcc cttttcttgc aataaacggt agatagcaac
     7861 cgaagtcgct agaatggctg gttgcgtata gcgggtctga ttgagtttgt cttcttccgt
     7921 atcgatgaga taacgcaaat cataacctag cacctgactc gctcgatcaa tcgtttcttt
     7981 aacaatcgga tactgatcat agaaatcccg tcccatccct agatactggg caccttgacc
     8041 agcaaataaa aaggctgttt tagtcatttc ttacaactcc tgtccagcga gaggcttctt
     8101 cttgaatttt cttagcggct ccgtaataca aatcttttag gatttcttca gctgtttctt
     8161 ctttagaaac aagccctgcg atttgacctg ccatgacaga gccaccctcc acatcaccgt
     8221 gaacaactgc tttggctagg gcaccggctc ccatttgttc aaagatttct aaatcaggat
     8281 cttcctgctt aaaggcatct ttttcagcca gttcaaaatc tctagtcaac tgatttttaa
     8341 tagcacgaac agcatgacca aagtgctgag ctgaaatcgt agtgtcaata tcccttgctt
     8401 ttaaaatttt ctccttgtag tttggatggg cattcgactc ttttgcaact acaaaccgtg
     8461 tccccacctg tacagcctct gcacctagca taaagccagc cgcagcacct tcaccatccg
     8521 caattcctcc tgcagcaata acaggaatag atacagctgt ggctacctgt cgcaccaagg
     8581 tcatggttgt taatttaccg atatgccccc cagcttccat tccttctgca ataacagcgt
     8641 ctgcaccgat tttttccatg cgtttagcta aagcgacact aggaacgaca ggaataacga
     8701 ttatcccagc ttcatggaaa cgttccatat acttgcttgg atttcctgct cctgttgtga
     8761 caactttaac accttcttca ataacgagat ccacgatatc ttccacaaag ggagataaga
     8821 gcatgatgtt gaccccaaag ggtttatcag tcaatgattt gattttatca atattggcct
     8881 tgacaacttc tttcggggca tttcccccac cgataattcc taatcctcca gccttggaaa
     8941 cagcccctgc caaatcacca tcagcaaccc aggccatccc tccttggaaa ataggatagt
     9001 caatcttcaa taattctgta atacgcgttt tcatagtgcc tccaaccttc cttgcttacg
     9061 taatagttcg atttcaccat aatttgacag tcaaactatt acctaaacaa gagggagtgg
     9121 gtttctccct actccttcta ctaatattct gcttattttg cttgctcttc aacgtaagca
     9181 accaagtcac caactgtttt caagtcattt tctgcttcga tttggatatc aaaagcatct
     9241 tcgatttctg agattacttg gaacaagtcc aatgaatctg cgtccaaatc atcaaaagtt
     9301 gattcaagtg ttacttctga tgcgtctttt ccaagttctt caacgataat ttcttgtact
     9361 ttttcaaata ctgccatgat aggactcctt taaaataaat agttttttta taacaatgtg
     9421 ttcaccacat gattacctaa attgtaagaa tgagcgtgcc ccaggtcaag cctccaccga
     9481 agcctgatag aagaacagtc tggctaccat ctaaagggat gagaccttgt tctacacact
     9541 ctgaaagtaa aatcgggata ctggctgcac tggtattgcc atattccatc atattggctg
     9601 gaagtttggc tcggtcaaca ccaatttttc tagccatctt atccaaaata cggtcattgg
     9661 cttgatgaag tagcagataa tccaagtctg tcacctctat aggagattca tcaatagtct
     9721 gcttgataga cttggctaca tctcgaatgg caaaatcaaa gactgtgcgt ccatccatct
     9781 tcaaaaatga atctgcactt tcttgatctg aaaatggaga atgcaaacct gaatgcccat
     9841 aagttaaaca ctcgctgcga cttccatcgc tattgagact ctcagctaag aaatgctctt
     9901 gctcgctagc ttctaacaag acaccaccag caccatctcc aaacaacaca gctgttgatc
     9961 gatccgacca atcgactgcc ttagagaggg tttcactacc aatcaccaag cctttttgaa
    10021 agcgaccaga agcgataaac ttttcagcag ttgaaagagc aaatacaaat ccactgcaag
    10081 ccgcggttaa gtcaaaagca aaggctttat tagcaccaat attagcttga acacgagcag
    10141 ctgtagaggg catcatcgaa tctggagtaa tggtagctag gatgataaaa tccagttctt
    10201 ctcctgttat tccagctttt gccatcagtt tcttagcaac ctctgtagcc aaatcactgg
    10261 tagattctgt tcttgaaata tgcctttgtc gtattcccgt tcgacttgaa atccactcat
    10321 cattggtatc cataatctga gccaagtcgt gatttgtaac cacttgctct ggcacataat
    10381 gagcaacctg acttattttt gcaaaagcca ttatttcaaa tcctccaaaa attggtaaag
    10441 attagtcaag cctttaccca tgacagcaat ttcttcctcg ctcatgccat caataatttt
    10501 ttctaccatg gccttgtgga agcgtttatg cagtctatga atcaagcgac ccttctttgt
    10561 caaatgcaga tgcaccacac gacgatcctg ttctgaccga actcgctcaa tgtagccctt
    10621 acgctctaaa ttgttcaaac ttgtcgtaac agttccaaga gttaccatca actcttttga
    10681 cacttgactt ggagtcacat ctggagcctt accgatgaca tcaatcgtat gcatttcttt
    10741 gatggagata tccttaaaac gactacctct caagttcact tcctcaatta caaggacatt
    10801 gttaaatata gatgttaaat attcattgat tcgttggtag tccatttttc atatccctcc
    10861 ttcttcaaaa aacttttaca atcaaaacat ttgacagaaa gaattatatc aaaattcaaa
    10921 gaatgatgca agtatttttt attttcctgt aaatttaggt cttcttctct ccgaatgagc
    10981 tcgcactcct tctttgaaat cctctgtttg agctaaggat ttctgtaggt tcagttctaa
    11041 agtagcgtaa ccctgccagt ctttaaattg gctctcccaa accaacttct taatggcagc
    11101 ataggaatta cttgaagcac gtcttaattt tttaagaagc tgttctctcg tcttttcaag
    11161 tttttcagct tcagaaacgc ggtaaaccaa cccccattct agggcttttt ctgccgttaa
    11221 agcctctcct gtcatagcta attgagcagc acgcgtcaca ccgatactgc ggctcaagag
    11281 atgaattccc cctgcatctg gagccaaacc aacaccaaca aaggcttgga taaacttagc
    11341 cttatccgtc gccaaacaga aatctgccgc aacagccata ttcgctgcgg cacctgcaac
    11401 agcaccgtca acttccatta aaacaggttt ggctatttgc ttgattttat aagaaatcgt
    11461 attgaccaac tctgcgattt ttgtcaatga tggaatatca tcctcatcca ctgcccgctt
    11521 catctctacc aaatctcccc caactgagaa gaccttccca ttggcattga ttaagataaa
    11581 atgcacagct ggattttctt ctgccaaagt cagagcttct aaaatctcct cacacatggg
    11641 aatatgaaaa ccatttgcga cctcaggacg gttcaaggta aggattgcca agtcctcttc
    11701 aagctgataa ataatgtgtt ccatcagggc tcctttttat tttataagca gtttatactc
    11761 ttcgaaaatc tcttcaaacc gcgtcagctt ccatctgcaa cctcaaaaca gtattttgag
    11821 ctgacttcgt cagtcttatt tacaacctca aagcagtgct ttgagcagtc tgcggctagc
    11881 ttcctagttt gctctttgat ttcattgagt gttaaattat ttcactgtca aagtatatct
    11941 tttttaggag cagaaagcaa gaaaaaatcg actgaaagtg tctcagccga taagaaaggc
    12001 tcgctttagg ctagaaagca tcttattttc ttcattgaaa aaagcctttc tttctgctat
    12061 aatcgtataa aatacttact ttaggagttc ttatgaaagt tgttaaattt ggaggtagtt
    12121 ctcttgcctc tgctagtcaa ttagaaaaag ttttaaacat cgtcaaaagc gattcagagc
    12181 gtcgttttgt agtcgtttct gcgcctggta aacgcaatgc tgaagatact aaggttacgg
    12241 atgccctgat taaatactac cgcgactatg ttgcgggtaa cgatattagc aagagccaaa
    12301 gctggattat cgaccgctat gctgctatgg ttagtgaatt gggactaaaa ccagctgtgc
    12361 tagaaaaaat ttctaaaagt attcacgcct tggccactct tcctattgaa gaaaatgaat
    12421 ttctctacga tactttccta gcagccggtg aaaataacaa tgccaaattg attgctgcct
    12481 actttaacca aaatggtatc gatgcacgct atatgcaccc tagagaagct gggattgtgg
    12541 tcacaagtga acctggtcac gctcgcatca ttccatcaag ttatgacaag attgaagaat
    12601 tgacaaacac caatgaagtc cttgtcattc ctggtttctt tggtgtcact aaggaaaatc
    12661 aaatctgtac tttctcacgt ggaggttctg atattacagg ttctatcatt gctgctggtg
    12721 tcaaagctga cctctatgaa aactttacgg acgttgatgg tatctttgca gcccaccctg
    12781 gtattatcca ccaaccacac tcgattcctg agttgaccta ccgtgaaatg cgcgagttgg
    12841 cctatgcagg cttctcagtc cttcatgacg aggctcttct tcctgcctac cgtggaaaaa
    12901 ttcctctggt tatcaagaat accaacaacc ctgaccatcc aggtactcgt atcgttctaa
    12961 aacacagtaa tgatgaattt ccagttgtgg gaattgctgg tgactcaggc tttgtcagca
    13021 ttaacatgtc gaaatacctc atgaaccgtg aggttggatt tggccgcaag gttctgcaaa
    13081 tcctggaaga acttaacatc ggttgggaac atatgccaac aggtatcgac gatctttcta
    13141 tcattctccg ttctcgccaa ctaactccca tcaaggaaga agaaatcctg cgtcagttgg
    13201 ttcaaaaggc taaagtagat catgcagaaa tcgaacacga cctttctatc attatgattg
    13261 ttggtgaaaa aatgaagagc cacatcggag tgactgctac tgcgacacgc gctctatctg
    13321 aaaacaaaat caacatccag atgatgtctc aaggttctag tgaagtttct attatgtttg
    13381 ttgtcaataa agaccaagag aaagcagcaa ttaaagccct ctacaatgcc ttttttggag
    13441 aaagtaagga agactaagca tggcccaatc acttaacaaa acagtactcc tcagtacgac
    13501 aggcacttcc tacctctcta tcgctgggaa agttgggaaa ttccttgtcg gagatcaggc
    13561 tttggaattt tacccagatg tcaatgtcga acaatttatc cagattcctt ggagccatat
    13621 caatcagatt ggagccaatg tcactggacg caaaatcagc cgccacttcg aagtctttac
    13681 agacaaagga aaattcctct ttgcttcaaa agactcaggt gccattctca aaattgcacg
    13741 cgaaaaacta ggcaatgaca aggtcgtcaa acttccgacc ctgattcaga caattagcca
    13801 aaaatttaaa aatctatttg caaaaaagta gaaactttag tataatcata gcaacggata
    13861 agtaggttat cttcttcttt cagaaagtct gcgggtactg cgagcagata ggagggatag
    13921 gtgaaattct accgttagta acaattacat acacaatcaa gtgcacacct gtgaagttgg
    13981 atggaaccgt ggccctgcca ctccaacgtt ttgtcaggcg tgcttttttc ataaaggagt
    14041 tcttatgtta gatatcaaac gtattcgtac agattttgaa gctgtcgcag aaaaattagc
    14101 tacacgtggt gtagatgctg ctgtcttgaa tgaaatgaaa gaaatcgatg ctaaacgtcg
    14161 taacatcttg attaaggttg aaactctcaa agcagaacgt aacacagttt ctgctgagat
    14221 tgcccaagct aagcgcaaca aggaaaatac agatgacaag attgctgcca tgcaaaatct
    14281 atctgctgag gttaaagcct tggatgctga attggcagaa atcgatgcta aattgacaga
    14341 atttacaacg actcttccaa atatcccagc tgacagcgtt cctgttgggg ctgacgaaga
    14401 cgacaatgtg gaagttcgcc gttggggtac tccacgcgag tttgacttcg aacctaaagc
    14461 tcactgggat ctcggtgaag accttggtat ccttgactgg gaacgcggtg gtaaggtaac
    14521 aggcgctcgc ttcctcttct ataaaggcct cggtgctcgt ttggaacgtg ctatctacaa
    14581 ctttatgttg gatgaacatg gaaaagaagg ctatactgaa gtcatcacac cttacatagt
    14641 caaccatgat tctatgtttg gtactggtca gtatccaaaa tttaaggaag atacttttga
    14701 actcagcgat accaactttg tcttgattcc aactgctgaa gttcctctga caaactacta
    14761 ccgtgatgaa atcttagacg gcaaagatct tccaatctac ttcactgcca tgagtccgtc
    14821 attccgttct gaggctggtt ctgccggtcg tgatacgcgt ggcttgatcc gtttgcacca
    14881 attccacaag gttgaaatgg tcaaatttgc caaaccagaa gaatcttacg aagaattgga
    14941 aaaaatgaca gccaacgctg aaaacattct tcaaaaactc aaccttccat accgtgtcgt
    15001 tgctctctct actggagata tgggcttctc agctgcgaag acttacgact tggaagtgtg
    15061 gattccagca caaaacaatt accgtgaaat ctcaagctgt tcaaacacag aagatttcca
    15121 agcccgtcgt gcccaaatcc gttaccgtga tgaagcagat ggcaaggtga aactccttca
    15181 taccttgaac ggttctggac ttgcagttgg acgtacagtg gctgcaattc ttgaaaatta
    15241 ccaaaatgaa gatgcttctg tgaccatccc agaagcactt cgtccataca tgggtggagc
    15301 tgaagtcatc aaaccataaa aaataaggtt tagctatttc tagctagacc ttttttcgta
    15361 accaaatcag ataagcacct agtacaaaga ataaaatagt taggcatata atggtttcag
    15421 ccaataccag gtaatccaga aatggaagtt tcaaaattcc ctgagccatc ttgagcgagg
    15481 tcgctgtgat aatggttggg aaggtgaggg ctgagaaggc tggttgaaaa ccttgtttta
    15541 aaatgttggg cagacgagtt aaaacaaaga aaaagaagga ttgagaagcc aaaatcatga
    15601 caatcaagac ccaagtcggc aggctggttc ctcctactcg aactagagaa gccaagagta
    15661 gagagaaagg agcacagtag attccttctt gtccaagcaa ggctagtggg agtggatgtt
    15721 tctttaaatc gctataaata aggggataga gatagaaggt caagagaaaa ccaaaactca
    15781 aggtcgcata ggcaatttcg ataataccta ccagaggata ggtcaaggca gccactgcta
    15841 tccccacata gagaaccgtc caacttggag tggcatgaac cctccgccct ggacaagcaa
    15901 acttgatggt aaaaccagca atcaaggtca aatccaagag aaatgaaaac caccaaatcc
    15961 cttgtgctac caaaggaaga taagagaata cgcgaaagac ataggtcgat aaaatcatcc
    16021 cagccatagg aaaggttgcc attcctgaca aaagaggggg cttggtcaat tcttgcttgg
    16081 tttctttcca attaaagaga tgcagaatta gaaagtaaat ccataaaacc aaaccagtca
    16141 gactaaaaag atgggataga accggcaacg tatctaaaat aagatttcca gctcctgcca
    16201 aacctagcaa acaacctgaa aatactaagg ggagtttttt catcctaacc tccaataatc
    16261 atgttagttt cagtataaca taaaagcgct taaatgagga tttaaaaaaa cgaagtccgc
    16321 ttatttcaga cttcatttta ctcagatatg aattaggcat aaggttgcaa ttctggatta
    16381 attggtgtat tagctaagtt gttggcatag ttacagagga ttgctaggct gacaccaaaa
    16441 accacatcca aggcattttg ttgagtgtag ccagcttcta aaaactcaga caaggcttca
    16501 tctcctacac gacccttggt attgataact gccaaggtaa acttagctag ggtatccaat
    16561 ttaggatctg tttcaattgg agtacgattg cgaagagctt gaatcaagtc atcattcatc
    16621 tggatttgtt tgatggaaaa ggctgtgtga cctgcgacac agaaggcaca accattggtc
    16681 acggctgccg tgatttgcac cacttcacgc tcaacgggtg tcaggctgtt gcgacggtgg
    16741 atagatgaga caatttgata ggcttctaaa acagtcgggg cattggccaa gagaccgatt
    16801 aggttgggaa tatagccatt gttgtctttt tctactgttt caagaatttc tttcacttct
    16861 gctggtgctg actctactgt atggatagta aatgttgtca taagatacct cttttcttat
    16921 tattgacact aatattattg gaaaatctta taaaatcctg attcctaagt ttatctaaga
    16981 taaagcttta ttctctcata agattttcgt tgttatatta gtttatcaca cttccaatca
    17041 cttgcataat atatattata tatcaggctg ataaaaatta tttataggca aaaaaatcac
    17101 acgagctgtg tgattccatt atttgtcaaa atacttttta gtttcagcaa taacgactgg
    17161 cgacaagacc aagagggcaa tcaagtttgg cagagccatc aaggcgttaa cgatatctgc
    17221 gataatccag accatatcca actcgataaa tcctcctaac aagaccatga gcacaaaaac
    17281 cacacggtag agccagataa agcgaacccc aaagaggaac tcaaaacagc gttctccgta
    17341 atagttccaa cctagaatcg ttgtaaaggc aaaaagtaca aggaagatgg tcaagagggc
    17401 aggcccaaag tgtgaaaaga ctgttgagaa agctgactga gtcaaggcaa ccccattcaa
    17461 gtcaccactc caaactccag ttaccaagat ggtcaaacca gttagagtac aaatgatgag
    17521 ggtatcaata aaggttcctg tcatggaaat caaaccttgc tctactggtt catttgtctt
    17581 ggcagctgca gctgcaatag gagcagaacc cagaccagat tcgtttgaga acacaccacg
    17641 cgccacacca ttttgaatag ccatccgaac gctagcacca gcaaatccac ctaccgcagc
    17701 aaggggacta aaagctgagg taaagactaa agcgattgtg ccagggattt ttccgatatt
    17761 aaagaaaata actgtaagag ttcctaagat ataaatgatg gccataaaag gaacaacagt
    17821 agttgaaacc ttagaaatag acttgagtcc accaaagact gcaatcgcta caaagacaga
    17881 caagacgaga gctgtgatgg ctggcgaaat cgtcgttgta ttttggatag attctgcaat
    17941 cgagttgact tgggtgaagg ttccgattcc caagagagca accaatactc ctgctactgc
    18001 aaacaaaaca gcaagtggtc gccacttttc tcccatccct agaaggatat aatgcatggg
    18061 acctcccgct actgcaccat ggtcgtcctt ggtgcggtat ttgatggcca agagtccttc
    18121 cgcatacttg gtagccattc caaagaaagc cgccatccac atccaaaata gagctcctgg
    18181 tccaccaacc ttgatagccg tcgcaactcc tatgatattt cctgttccaa cagttgatgc
    18241 caaggctgta cacagagctg caaaactgga tacatcacca tgtcccttat cctggataaa
    18301 aataagctga aaagccttgg gcagacgcaa aacctgcaag agtcctagcc gaatagttag
    18361 gtaaatccct gttccgacca ataaaatcaa gaggggcggt ccccaagcaa aagcatcgat
    18421 tgatttaagc aattctaaca tttccttctc ctatcgtttc aaccccaaaa gaaagagcac
    18481 atgcaagata catgtactct ggaatgctta gataaatgct aaaaagcggt ctatcctagc
    18541 tctgtccttt tacctgagag tttgagcagt tgcctgcctt gccccttcgg tgcctttacg
    18601 gtctctccag agttccgtcc atttacagtc atggaaaatc aaacgattcc ccacttctat
    18661 taaacttcat tcggtgttgg tatttaattg attctaattt tacaaaaaat gttggctttt
    18721 gtcaatgtgt ttattagtaa aaattagttc aacagttttt actttataaa gtccagaata
    18781 ctgctatcct ttaaaagtga caatagtcgc accactgcct ccagcatttt gtggggcata
    18841 gccgaaactc ttgacatgtt tgtttctttg caagtatttg gtaactcctt cacggatgac
    18901 tcctgttccg ataccatgga tgatatcaac ttgagccata ttgttaagca aggcttggtc
    18961 gatgaaggta tctagctcat tcatggcttc ttcatagcgc ttgcctcgaa gatccagtct
    19021 agcttgaggt cctcgcccag aagttcgttt cacaacattg acctgtttct tcttgactgg
    19081 tttttcttgc tgggcttgaa caagatcaaa ctctttctct tccaaggtca tcttaatcaa
    19141 gccaacttgg gcttcccagc ggccgtcctt gagttgactg gtcaaggtac cacgctgacc
    19201 ataactgaga accacgatat catctcccac ctttggagct cgttttttct tggccttttg
    19261 aaggaccttg tttttagaca agtccacttt ttcaggagcc aattttttca acttggcctt
    19321 ggcttcaatg atttcgtggg gcttgagttg ggatttactg tggagatttt tgagaatctg
    19381 gtcactttca cttagggcca tatccacaat ctcagcagcc tgttcacgcg ccttgttaag
    19441 ctcggtttcc ttttcacgat taagctcgtt gtagagtttt tttagcgcac ggttcatctt
    19501 gagattttct tgctccacct cacggatatt gtccaagcgt ttgcggcttt ccagcgtctg
    19561 ctcttctaat tgctcaatga tacgattgac gtcattgtcc tgatcgatct gctgactggc
    19621 atctcctacg ataacttcag ataggcctag acgtttggca atttcaaagg cattacttcg
    19681 gccaggaaca ccctgcataa agcgataggt cgggcgaaga gttgcagtat caaactccat
    19741 actggcattt tgcacaaagg ctgtctcaat accgtaggcc ttgagttctg gatagtgggt
    19801 cgtcgccatg gtcttgattt gacgcaggcg aaggtcctcc agaatagcca tggcaagggc
    19861 tgctccctct tggggatcag taccagcccc caactcatcc aaaagtaaga gtgaatgttg
    19921 gttgaccttg ccaagaatat ccacgatatt ggtcatatgg ctagagaagg tagacaagct
    19981 ctgctcaata gactgctcat ctccaatatc agcaaaaatt tcttcaaaaa taccaacacg
    20041 acttcccttg tctgctaaaa tcggcaatcc tgactgggcc atgacctgtg tcaagcccag
    20101 agttttgagc atgatggtct tcccacctgt attgggacct gtaatgacaa tagctgttaa
    20161 atcttgacca aaatagacat catttgcgac ggcatttttg accaaaggat ggcagacatg
    20221 gagcagttga atctcttgat tttctgacag ctgaggcacg actgcttgtc tttcttggat
    20281 aaatcgaacc ttggcacgaa tcaagtccag atgaccgata atccaagcat cattagcaat
    20341 ctcagccgca tgagggcgga cacgctcaga aatttcttgg agaatgcgaa gcatttcata
    20401 gcgctcatct gctcgcagac tagcaatttc ttcgctcagt ttgactacct cacggggttc
    20461 gatatagacg gtgtttccac tagcagaaat atcatgaacg acacctgcaa tcttattgcg
    20521 gtaggtgttt ttgactggta aaaccttacg gccatttctg ctagcaacaa ttccttccgt
    20581 caacatctgc gctttttgct tgagcaagtc ttgtaaaaca tcgcgtacct gactctcgct
    20641 atcatgtatt tttcgacgga ttcgcgccaa ttcttcactg gcaaaatttt caatgaaacc
    20701 cgcatcatta aaggcctgaa gatttccttg taattgcgga aaatcatgta atttctcaaa
    20761 ccaaagggct aattcttcca agctgacatt ttccagattg gcgtaaaaac tttgaagttc
    20821 tcggctggca agaagcacgc gtttcaagag taggaactcc tcgatattga gatccgctcc
    20881 catctccaac ctcttgcaga ctcctgcaat ttccttagtt gagagaatag taaaatgcgg
    20941 ttgctcgacg aaaagagcct gcatttcctt catctcagca aaagcctgtt tgattttatc
    21001 tgctttggca gtcggagcca gttgtctcaa ttgctccaag ccctgctcgg tcaacaaatg
    21061 aggctcaaac aaggccttga ccttatcgaa ctctaatgtt tctaatattt tcttattcat
    21121 ctttatttcc ttgtctttga atgtctaggt gtggtaactt tttacattct gatagattca
    21181 agtcaaatct gtaaacaaag ggctaggaaa actcctgccc tttttatccg attaaatttg
    21241 tcacccagat ttgtttgagc caactggttg ttatcggtat gctctggatg atgtgttttg
    21301 cgacgatact cttttcaaga ggattttgta taactgccat ggggatggtc gccaagattg
    21361 tcaaggccat ttgcaagaca aataaggtca ccaacatgga caagatacct gctgaaactt
    21421 ggaacaactt accacccagt tttttgctag gaatcaagtg taagagaaga ccaagcaaac
    21481 gaccaatgct atagacaatc ccaaatacaa gcaagtagcc gatacctgca taaaagacct
    21541 tatccagctg aaagagttga tccgatggga aaaagaaagt cccctgacct tcctgcgaat
    21601 ttgcataagg gagcaataaa tggaattgct ctccaagccc cttataaaac tggccagcca
    21661 taaaagccga tgccatggct gaaatcaggt aataaatctg taagagcagg cctctccgat
    21721 agccgatata aaatccccaa accaagacca atagaagaag gaatgaaatc ataaggaatc
    21781 ctcaatcttg ctctgttctt gcttacaagt cacaagcttg tgacggagtt cttctagctc
    21841 ttgctcctta tcgtcaaatt caatctcacg gctgagctga gttgataaac agttgactgc
    21901 caacaaaaga gcgattgttt catcatctgc gctaggcatt tgttctttaa ttgcttggta
    21961 tttttctgtc gcaaccttag cgatttcctc cataaaaagg ttatcatgtt cacttgtcaa
    22021 ggttaacgat tttttcccga atgtaaattt gaatcgattt agatttgcca taaaattcac
    22081 ctcacgatat tataccaaaa ttcgctaatt ttgtcagttt ttacaaattt tactgctttt
    22141 gtggtacaat agaaactatg gcaagtatca cactcacacc aagcgaaaag gatattcagg
    22201 cttttcttga acactatcaa accagtctgg ctcctagcaa gaatccctat atccgctact
    22261 ttttgaaact acctcaagca acggtttcta tctatacttc tggaaaaatc ttgcttcagg
    22321 gtgaaggggc tgaaaaatac gccagtttct ttggctatca agctgtagag caaaccagcg
    22381 gacaaaatct tcctttgatt gggacagatg aggtagggaa tggttcctac tttggtgggc
    22441 ttgcagttgt ggcttccttt gtcacacctg accagcatgc cttcttacga aaactcggtg
    22501 tgggagattc taagactctg accgaccaaa agattcgtca gattgctcct attctcaagg
    22561 aaaaaattca gcaccaggca ctccttctct cacccagcaa gtacaacgag gtcatcggag
    22621 accgctacaa tgctgtttcg gttaaggttg ccctccataa tcaggctatc tatctcctcc
    22681 ttcaaaaagg tgttcagcct gagaaaattg tgattgatgc ctttaccagt gctaaaaatt
    22741 atgacaagta cttggcacaa gagaccaatc gtttcagcaa tcctatcagc aatcctatca
    22801 gcttagaaga aaaggctgag ggcaaatact tggctgtcgc agtttcttct gtcattgcgc
    22861 gtgatctctt tctggaaaat cttgaaaatt tgggacgaga actgggttat cagcttccaa
    22921 gtggagctgg aacggcttct gacaaggtgg ctagccagat tttgcaagcc tatggtatgc
    22981 agggactcaa cttctgcgcc aaattgcact ttaaaaatac tgaaaaagcg aaaaacgctt
    23041 agaaaggtaa gttatgaatt tatttaaaaa tttcttaaaa gagtggggat tattcctcct
    23101 gattctgtca ttactagctt tgagccgtat ctttttttgg agcaatgttc gcgtagaagg
    23161 acattccatg gatccgaccc tagcggatgg tgaaatcctc tttgttgtta agcacctccc
    23221 tattgaccgt tttgatatcg tggtggccca tgaggaagat ggcaataagg acattgtcaa
    23281 acgtgtcatc ggaatgcctg gcgataccat ccgttacgaa aatgataaac tttacatcaa
    23341 tgacaaagaa acggacgagc cttatctagc agactatatc aaacgcttca aggatgacaa
    23401 actccaaagc acttactcag gcaagggctt tgaaggaaat aaaggaactt tctttagaag
    23461 tatcgctcaa aaagcccaag ccttcacagt tgatgtcaac tacaacacca actttagctt
    23521 tactgttcca gaaggagaat accttctcct cggagatgac cgcttggttt cgagcgacag
    23581 ccgccacgta ggtaccttca aagcaaaaga tatcacaggg gaagctaaat tccgcttctg
    23641 gccaatcacc cgtatcggaa cattttaaga gacctaagag gccgagaatc accaatctca
    23701 gcctcttctt ctatcgtgag aaaatgattg gtaactatct aaacttacca gaacagaaac
    23761 acctcaactc tcacctattc atgcaaagga attttatgga agtttatttt tcaggaacca
    23821 ttgaacggat tatttttgaa aatcccagca atttttatcg catcctcctc ctagaaatcg
    23881 acgatacgga cgcagaggat tttgatgatt ttgaaatcat tgtcacagga accatggctg
    23941 atgtaattga gggcgaagac tatacttttt gggggcaaat tgtccagcac tccaagtatg
    24001 gagaacaact gcaaatcagt cgttatgatc gcgcaaaacc aactagtaag ggcttggtca
    24061 agtacttttc aagtagccat ttcaagggga ttggtctcaa gacagctcag aaaatcgtgg
    24121 atacctatgg cgaaaatacc attgacgaaa ttttgcaaca cccagaaaag ttagaaggca
    24181 tcgcaggact ctctgccaaa aatcgcgagg ctttcgtctc cactctccgt ctcaactacg
    24241 gaacggagat ggttttggcc aaactagcca actacggcat tcccaacaaa ctagcctttc
    24301 agattcaaga cttttacaag gaagaaaccc ttgatgtggt tgaaaattat ccctaccagc
    24361 tggttgagga tatcaagggt ttgggcttta ccattgctga ccaactagct gaggaactag
    24421 gcatcgaaag tcaggctcct gaacgcttcc gcgccggtct agttcacagt ctttttcagg
    24481 cctgtatgga aacaggggac acctatgttg aagcacggga tttgctggaa caaaccctta
    24541 ctctccttga gtcttcccgt cccgtggaac tagaccccag ccaagttgcc caagagctct
    24601 cctacctgat cgaagaagac aaggttcagc agattgatac caagatcttt gacaacagcc
    24661 tctttttcgc tgaggaaggc atccgcagtc acttggttcg tatccttgaa aaaggaaaac
    24721 agaagagtca tgatttaaaa accattcaaa aacatatcac tactatcgag aaagagcttg
    24781 gaattgagta tgataacatt caaaaacagg ctatctgtga tgctatccag aataaggtct
    24841 ttatcctgac aggtggacct ggtactggta agacaactgt tatcaatggg atcatcgctg
    24901 tttacgccct gttagaggga cttgacctca ggaagaaaag caacctgccc attcttcttg
    24961 ctgctccaac tggtcgagct gctcgtcgca tgaatgaatt gacaggtttg cctagcgcga
    25021 ccatacaccg ccacttggga atgacaggtg acgatgatac cagtcatctg gaagattacc
    25081 tagatgctga ctttatcatc gtggatgaat tctctatggt ggatacttgg ctggccaatc
    25141 aactcttctc cagtatctct tctaacagta agatcctcat cgtaggtgac agtgatcagc
    25201 taccttctgt cagtcctgga caggttctag cggatctgct tcatattcct ttgattcctc
    25261 agactcgctt ggaaaaaatt tatcgacaaa gcgaagaatc aaccatcgtc accctagcta
    25321 gtcagattcg acagggcatc ttgccagctg atttcaccca aaaaaaagct gaccgttcct
    25381 actttgaaat tgctagtggc catattcctg ccaccattga aaaaatctta ggtgccgccc
    25441 tcagaaatgg tattcctgcc cgtgatatcc aagttctggc tcctatgtac cgagggacgg
    25501 cagggattga tgctatcaat cagctcatgc aagacctgct caatccccca caaaaagatc
    25561 aactcagttt tgaagctcct cagtgccact atcgtaaggg agacaaggtc attcatttgg
    25621 ttaacgatgc tgaaatcaat gtctttaatg gggatttagg agctatcaca gacctgattc
    25681 ctggtaaata caccgaatcg aaacaagacg agattgtcat tgattttgat ggtaatgaag
    25741 tctcttaccc ccgtaacgaa tggtacaaga ttcgcttggc ctatgccatg agtattcata
    25801 agtctcaggg aagtgagttc ccagttgtca tcctaccgat tactagtgct agccgacgta
    25861 tgctggagcg aaatctcatc tacacagcca ttacacgtgc caaaagcaaa ctcatcttac
    25921 taggcgaatt acaagccttc gactatgcta cccaacacat cggaactgcc cgaaaaacct
    25981 atctgattga acgcttcagt gatctactgg agaatgttga agaaaagcaa caagctgtct
    26041 ctgaaacagt cacatcaagt gcctctgaac aatcctacat cctaaccgaa gaaaactggg
    26101 acagcattcc agccatgatt gggattacag acacagacct caaagagatt tttggaaaat
    26161 a
//
LOCUS       DAVLYR010000012        23763 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLYR010000012 DAVLYR010000000
VERSION     DAVLYR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.012"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            161..1432
                     /locus_tag="VYO63_000462"
     CDS             161..1432
                     /locus_tag="VYO63_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1671442.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1543..2835
                     /locus_tag="VYO63_000463"
     CDS             1543..2835
                     /locus_tag="VYO63_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1671443.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2837..3679
                     /locus_tag="VYO63_000464"
     CDS             2837..3679
                     /locus_tag="VYO63_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1671444.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3940..4740
                     /locus_tag="VYO63_000465"
     CDS             3940..4740
                     /locus_tag="VYO63_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1671445.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PGQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4750..5736
                     /locus_tag="VYO63_000466"
     CDS             4750..5736
                     /locus_tag="VYO63_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1671446.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            5872..5952
                     /locus_tag="VYO63_000467"
                     /pseudo
     CDS             5872..5952
                     /locus_tag="VYO63_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6162..7103)
                     /locus_tag="VYO63_000468"
     CDS             complement(6162..7103)
                     /locus_tag="VYO63_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1671447.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(7081..8844)
                     /gene="aspS"
                     /locus_tag="VYO63_000469"
     CDS             complement(7081..8844)
                     /gene="aspS"
                     /locus_tag="VYO63_000469"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1671448.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(9196..9426)
                     /locus_tag="VYO63_000470"
     CDS             complement(9196..9426)
                     /locus_tag="VYO63_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671449.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(9444..10085)
                     /locus_tag="VYO63_000471"
     CDS             complement(9444..10085)
                     /locus_tag="VYO63_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1671450.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(10097..10831)
                     /locus_tag="VYO63_000472"
     CDS             complement(10097..10831)
                     /locus_tag="VYO63_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1671451.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(10834..11031)
                     /locus_tag="VYO63_000473"
     CDS             complement(10834..11031)
                     /locus_tag="VYO63_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671452.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQGDQFVWLYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(11031..11237)
                     /locus_tag="VYO63_000474"
     CDS             complement(11031..11237)
                     /locus_tag="VYO63_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1671453.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(11247..11561)
                     /locus_tag="VYO63_000475"
     CDS             complement(11247..11561)
                     /locus_tag="VYO63_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671454.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(11831..11911)
                     /locus_tag="VYO63_000476"
                     /pseudo
     CDS             complement(11831..11911)
                     /locus_tag="VYO63_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(11902..13191)
                     /locus_tag="VYO63_000477"
     CDS             complement(11902..13191)
                     /locus_tag="VYO63_000477"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1671455.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKEA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVSYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(13269..14516)
                     /locus_tag="VYO63_000478"
     CDS             complement(13269..14516)
                     /locus_tag="VYO63_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1671456.1"
                     /translation="MKLLFRNPAYRILILSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(14709..15560)
                     /gene="rgg"
                     /locus_tag="VYO63_000479"
     CDS             complement(14709..15560)
                     /gene="rgg"
                     /locus_tag="VYO63_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1671457.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            15684..16025
                     /locus_tag="VYO63_000480"
     CDS             15684..16025
                     /locus_tag="VYO63_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1671458.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            16083..16163
                     /locus_tag="VYO63_000481"
                     /pseudo
     CDS             16083..16163
                     /locus_tag="VYO63_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(16324..18027)
                     /gene="ilvD"
                     /locus_tag="VYO63_000482"
     CDS             complement(16324..18027)
                     /gene="ilvD"
                     /locus_tag="VYO63_000482"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1671459.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(18260..19192)
                     /locus_tag="VYO63_000483"
     CDS             complement(18260..19192)
                     /locus_tag="VYO63_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1671460.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(19189..20046)
                     /locus_tag="VYO63_000484"
     CDS             complement(19189..20046)
                     /locus_tag="VYO63_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1671461.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(20050..21396)
                     /locus_tag="VYO63_000485"
     CDS             complement(20050..21396)
                     /locus_tag="VYO63_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1671462.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFITKPSAKEE"
     gene            complement(21409..21693)
                     /locus_tag="VYO63_000486"
     CDS             complement(21409..21693)
                     /locus_tag="VYO63_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1671463.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(21697..23727)
                     /locus_tag="VYO63_000487"
     CDS             complement(21697..23727)
                     /locus_tag="VYO63_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1671464.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         7387 a         5212 c         4187 g         6977 t
ORIGIN      
        1 aaatgcaaaa ttatgctatc aaatcaagct taaaaatttt ttaaaatttt tacaaaaaat
       61 acttgcaacc gttttctatt tgtgctatac taagctcata aaggaaaacg tttgcgtttc
      121 cttatcaata aaacttgcta ttctttggga ggaatacact atgtcatcta aatttatgaa
      181 gagcactgcg gtgcttggaa ctgctacact tgctagcttg cttttggtag cttgcggaag
      241 caaaactgct gataagcctg ctgattctgg ttcatctgaa gtcaaagaac tcactgtata
      301 tgtagacgag ggatataaga gctatattga agaggttgct aaagcttatg aaaaagaagc
      361 tggagtaaaa gtcactctta aaactggtga tgctctagga ggtcttgata aactttctct
      421 tgacaaccaa tctggtaatg tccctgatgt tatgatggct ccatacgacc gtgtaggtag
      481 ccttggttct gacggacaac tttcagaagt gaaattgagc gatggtgcta aaacagacga
      541 cacaactaaa tctcttgtaa cagctgctaa tggtaaagtt tacggtgctc ctgccgttat
      601 cgagtcactt gttatgtact acaacaaaga cttggtgaaa gatgctccaa aaacatttgc
      661 tgacttggaa aaccttgcta aagatagcaa atacgcattc gctggtgaag atggtaaaac
      721 tactgccttc ctagctgact ggacaaactt ctactataca tatggacttc ttgccggtaa
      781 cggtgcttac gtctttggcc aaaacggtaa agacgctaaa gacatcggtc ttgcaaacga
      841 cggttctatc gcaggtatca actacgctaa atcttggtac gaaaaatggc ctaaaggtat
      901 gcaagataca gaaggtgctg gaaacttaat ccaaactcaa ttccaagaag gtaaaacagc
      961 tgctatcatc gacggacctt ggaaagctca agcctttaaa gatgctaaag taaactacgg
     1021 agttgcaact atcccaactc ttccaaatgg aaaagaatat gctgcattcg gtggtggtaa
     1081 agcttgggtc attcctcaag ccgttaagaa ccttgaagct tctcaaaaat ttgtagactt
     1141 ccttgttgca actgaacaac aaaaagtatt atatgataag actaacgaaa tcccagctaa
     1201 tactgaggct cgttcatacg ctgaaggtaa aaacgatgag ttgacaacag ctgttatcaa
     1261 acagttcaag aacactcaac cactgccaaa catctctcaa atgtctgcag tttgggatcc
     1321 agcgaaaaat atgctctttg atgctgtaag tggtcaaaaa gatgctaaaa cagctgctaa
     1381 cgatgctgta acattgatca aagaaacaat caaacaaaaa tttggtgaat aaaaaatttg
     1441 ttcaaggggg gtggaaatca aatccccctt tgaatttatc aatagagaca caaataattt
     1501 agctttctta taaaaaagta gtatcctatg aaaggagtta atatggaaaa gcaacaacct
     1561 agtaaagcag ccctgctgtc tatcattcct gggttaggac agatttacaa taaacaaaaa
     1621 gccaaaggtt ttatcttcct tggtgtaacc atcgtatttg tcctttactt cctagcactt
     1681 gcaacccctg aattgagcaa cctcatcact cttggtgaca aaccaggtcg tgataattcc
     1741 ctctttatgc tgattcgtgg tgccttccat ctaatctttg taatcgttta tgtactcttt
     1801 tatttctcaa atatcaaaga tgcacatacg attgcaaaac gcattaacaa tggaattcca
     1861 gttccacgca cactcaaaga catgatcaaa gggatttatg aaaatggctt cccttacctc
     1921 ttgatcattc catcttatgt tgccatgacc ttcgcgatta tcttcccagt tatcgtaacc
     1981 ttgatgatcg cctttaccaa ctacgacttc caacacttgc caccaaacaa gttgttggac
     2041 tgggttggtt tgaccaactt tacaaacatt tggagcttga gtaccttccg ttctgccttt
     2101 ggttctgttc tttcttggac tatcatttgg gctttggcag cttctacttt acaaatcgta
     2161 attggtatct tcacagctat cattgccaac caaccattta tcaaaggaaa acgtatcttt
     2221 ggtgttattt tccttcttcc ttgggctgtc ccagccttca tcactatctt gacattctca
     2281 aacatgttta acgatagtgt cggtgctatc aacactcaag tattgccaat cttggctaaa
     2341 ttccttcctt tccttgatgg agctcttatt ccttggaaaa cagacccaac ttggactaag
     2401 attgccttga ttatgatgca aggttggctc ggattcccat acatctacgt tttgactttg
     2461 ggtatcttgc aatctattcc taacgacctt tacgaagcag cttatattga cggtgccaac
     2521 gcttggcaaa aattccgcaa catcactttc ccaatgattt tggctgttgc ggcacctact
     2581 ttgattagcc aatacacctt caactttaac aacttctcta tcatgtacct cttcaatggt
     2641 ggaggacctg gtagtgtcgg aggtggagct ggttcaaccg atatcttgat ctcatggatc
     2701 taccgtttga caacaggtac atctcctcaa tactcaatgg cggcagctgt taccttgatt
     2761 atctctatca ttgtcatctc aatctctatg atcgcattca agaaactaca cgcatttgat
     2821 atggaggacg tctaagatga ataactcaat taaactcaaa cgtagactga ctcaaagcct
     2881 tacttacctt tacctgattg gtctatcaat tgtaattatc tatccactgt tgattaccat
     2941 catgtcagcc tttaaagcag gtaacgtctc agcctttaaa ctagatacta atatcgacct
     3001 caattttgat aactttaaag gcctcttcac tgaaaccttg tacggtactt ggtacctcaa
     3061 cactttgatt atcgccttaa ttaccatggc tgttcaaaca agtatcatcg tacttgctgg
     3121 ttatgcttac agccgttaca acttcttggc tcgtaaacaa agtttggtct tcttcttgat
     3181 catccaaatg gtgccaacta tggccgcttt gacagccttc ttcgttatgg cgcttatgtt
     3241 gaacgccctt aaccacaact ggttcctcat cttcctctac gttggtggtg gtatcccgat
     3301 gaatgcttgg ctcatgaaag gctacttcga tacagtgcca atgtctttag acgaatctgc
     3361 aaaactagac ggtgcaggac acttccgccg cttctggcaa attgttctac cacttgttcg
     3421 cccaatggtt gccgtacaag ctctctgggc cttcatggga cctttcgggg actacatcct
     3481 ctctagtttc ttgcttcgtg agaaagaata ctttactgtt gccgtaggtc tccaaacctt
     3541 cgttaacaat gcgaaaaact tgaagattgc ctacttctca gcaggtgcta tcctcatcgc
     3601 ccttccaatc tgtattctct tcttcttcct acaaaagaac tttgtttcag gacttacaag
     3661 tggtggcgac aagggataat ttatccccgc cacccttttt cattttatac tcttcgaaaa
     3721 tctcttcaag ccacgtcagc tttatctcca acctcaaagt tgtgctttga gcaacctgca
     3781 gctagcttcc tggtttgcac tttgattttc attgactatt agcgattgtc actgtaaata
     3841 atatccttgt agcaagcaat ttacccccta gacttgaaat aaagcgcatt tctctatata
     3901 ataatactca tatagaaaac accttttaga aagataccta tgcttccata tccattttcc
     3961 tatttttcaa gtatttgggg gtttcgtaag cccctgtcca aacgtttcga gctcaactgg
     4021 tttcaacttc tctttaccag tatcttcctt atcagcttgt ctatggtacc cattgctatc
     4081 caaaacagct cccaggagac ctatccgcta gaaactttta tcgataatgt ctatgaacct
     4141 ctgacaaatg aggttgtcca ggatctctct gaacatgcta caattgtcga tggcacatta
     4201 acttatactg gaacagctag tcaagcccct tctgttgtga ttggtccagg tcaaatcaag
     4261 gaattaccta aggacttgca actgcatttc gatacaaatg aactagtcat cagcaaggaa
     4321 agcaaggaac tgacccgcat ctcttaccga gccattcaga ctgagagttt caaaagcaaa
     4381 gacagcttga cccaagcaat ttctaaagac tggtaccaac aaaatcgtgt ctatatcagc
     4441 ctcttcctag ttctcggtgc gagcttcctc tttggtttga atttctttat cgtctctcta
     4501 ggagctagtc ttctccttta tatcactaag aaatcacgcc tcttttcatt tagaaccttt
     4561 aaagagtgct atcactttat cttgaactgt ttaggattgc cgactctgat tacacttatt
     4621 ttgggattat ttggccaaaa tatgacaacc ctgattactg tacaaaatat tctttttgtt
     4681 ctgtatctgg tcactatctt ttataaaaca catttccgtg atccaaatta ccataaatag
     4741 gagattttta tgcccgttac gattaaagac gtggccaagg ctgctggtgt ttcgccttca
     4801 accgtaaccc gtgttattca aaataaatca accattagcg acgaaacaaa aaaacgcgtt
     4861 cgcaaagcta tgaaggaact caactaccac ccaaacctta acgctcgtag cttggtaagc
     4921 agctatactc aggttatcgg attagttctt cctgatgact cagacgcctt ctaccagaat
     4981 cctttctttc catcggttct acgtggcatc tctcaagtcg catctgaaaa ccactatgcc
     5041 attcaaatag caacagggaa agatgagaag gagcgtctca acgctatttc acaaatggtc
     5101 tacggcaagc gtgtagatgg gctaatcttt ctctatgccc aagaagaaga ccctctcgta
     5161 aaactcgtcg cagaagaaca gttccccttc cttatcttag gtaaatctct atctcctttc
     5221 atcccacttg tcgacaacga caatgttcaa gctggttttg atgcgactga atatttcatc
     5281 aaaaaaggct gcaaacgcat tgcctttatc ggaggaagta aaaagctctt cgtgaccaaa
     5341 gaccgtttaa cgggctatga acaggcgctt aaacattaca aacttaccac tgacaacaat
     5401 cgcatctact ttgccgacga gtttctggaa gaaaagggct ataaatttag caagcgatta
     5461 ttcaagcacg atccacaaat tgatgctatc atcacaaccg atagcctcct agctgaaggt
     5521 gtttgtaact atattgccaa acaccagctg gatgtccctg ttctcagctt tgactcggtt
     5581 aatcccaagc tcaacttggc agcctatgtc gatatcaata gtttagagct tggtcgtgtt
     5641 tcccttgaaa ctattctcca gattattaat gataataaaa acaataaaca aatttgttac
     5701 cgtcaattga tcgcccacaa aattatcgaa aaataagaga ctgggcaaag agtcgttaaa
     5761 agcaaaaacg catactatca ggtattgaaa aaacttgata ctatgcgttt tattgtggga
     5821 agatttactt ccttttctac tgaaattgag tcttttccca agatcttttt atactcaatg
     5881 aaaatcaaag tgcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt
     5941 gtggatagaa ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac
     6001 gaagtcagct caaaacatgg ttttgaggtt gcagatagaa ctgacgaagt cagctcaaaa
     6061 catggttttg aggttgcaga tagaactgac gaagtcagta acatatatac ggcaaggtga
     6121 agctgacgtg gtttgaagag attttcgaag agtattaatc actaattatc tatctcaaca
     6181 aatcttccta gaatatgaac gttttccgag acagagacaa aggagcttgg atccacttgt
     6241 gtcataatct gtttaaattc attaaactct gcacgtgtaa tgacagtgat taaaactgcc
     6301 tttctctcgt gattataggt tccttctgca tcgtggatca tggttgctcc gcggtgcaat
     6361 tttttatgga ttttttcaat taccttctct ggatgatttg tcacaatcat ggcctgcata
     6421 cgcttttgct tagtaaagac tgcgtctgtc acacggctag agacaaagat ggtaatcata
     6481 gaataaagag cgtatttcca accaaaggtc aaacctgcta tcagcatgat agttccattt
     6541 accaagaaag aaatactacc gacattctta cccgttttct tgcgaatagt caggctgacg
     6601 atatccgtcc caccactgga gatattgttt cgaagagcaa aaccaatccc caaacccata
     6661 acaacacccc caaaaaggga attgataatg ggatcctccg tcaaggttgc cacagggaca
     6721 aactggataa agaaggaact catagatacc gtgataaagg taaagacggt gaacttatgg
     6781 ccaatctgat accaagctaa gaccatcaaa gggaagttaa tggcgtagaa gcttagcgaa
     6841 atcggaatat gaaaaccaaa ccagtgatta ctcaaggcag agataatctg tgccagacct
     6901 gttgcaccac tcgaatacac atgccctggt tggaaaaaga aattaactgc tactgctgat
     6961 aaaaaaccat agaccagaga ggccgaaatc ttctcatcat acttttctcg agagatactt
     7021 tgtaagacac gtaaaatttt tatctgataa gcaaagcggc gcagataata gcgccaccgc
     7081 ttaattcgtt ttgtttgttt catcttcttc tacttgtaag ctgagttcct ctagttgttt
     7141 gagagcgact gttgatggag cttgtgtcat tgggtcagtt gccttgttgt tcttaggaaa
     7201 ggcaatgact tcacggatat tttcttctcc agcaagcaac atgacaaaac ggtcaagccc
     7261 gatagccaaa ccaccgtgtg gtgggaaacc atagtccatg gcttcaagaa ggaaaccaaa
     7321 ctggtcattg gcttcttcag ttgagaaacc aagagccttg aacatgcgtt cttgaaggtc
     7381 tttttggttg atacgaaggc taccaccacc aagctcataa ccgttcaaga cgatatcgta
     7441 agcaatggca cgaaccttag ccaaatcacc ttctaattcg tgagcagtct cttcctgtgg
     7501 aagtgtgaat ggatggtggg cgctcatgta gcggccttct tcttcagacc attcaaacat
     7561 cggccagtca accacccaaa ggaagttgaa cttatcatta tcaatcaagc caagctcttt
     7621 agcaatacgt ccacgaaggg cacccagtgt tgcattagcc acttcaagcg tatccgccac
     7681 aaagagaacc aagtccttat cttcaagagc aagcgctgtt gtcaattctt cttggatacc
     7741 agtcaagaac ttggcaactg gtccgtttaa ttctccatca accaccttga cccaagcaag
     7801 acctttggca ccatactgtt tggctacttc cgtcatcttg tcgatgtctt tacgtgaata
     7861 gttgtccgca gctcctttga ccacaatcgc ttttacagca ggtgcttctg aaaagacttt
     7921 aaagtctaca cctttgacca cttctgtcaa gtcctgaagc aacatgtcaa aacgagtatc
     7981 tggcttgtca gaaccgtaaa gagccatagc atcatcgtat ttcatacgag ggaacggtag
     8041 cgttacttcg atgccttttg tttccttcat cacgcgcgcg atcaagcctt ctgtaatatc
     8101 ttggatttct ttctcagtaa ggaaggacgt ttccaagtcg acctgagtaa attcaggctg
     8161 gcggtctcca cgcaagtcct cgtcacggaa acatttaacg atttggtagt aacggtcaaa
     8221 accagcattc atcaagagct gtttcgtgat ttgtggactt tgaggaagag cgtaaaaatg
     8281 ccccttatta acacgagacg gcactaaata atcacgcgcc ccttcaggcg ttgacttaga
     8341 aaggaatggt gtctccacgt cgataaactc caactcatcc aaatagttgc ggatagagtg
     8401 ggtcaccttg gcacgaagtt taagattttc caacatttct ggacgacgaa ggtcaaggta
     8461 acggtaacgc aaacgtgtat cgtcatttgc ctcaatgcca tccttaatct caaatggtgt
     8521 tgtcttagct gtgttaagca caataagagc tgtcacgttt aactcaaccg caccagttgg
     8581 caacttatca ttggcttgct cacgcgcagc gacctgacca gtcacctcaa taacaaattc
     8641 gctacgaagg ctttcagctg ttgccataac ctctgcagat actttttcag ggttgataac
     8701 caactgcatg attccttcac ggtcacgaag atcgataaag atcaaaccac caaggtcacg
     8761 acgacggcca acccatcctt tcaaggttat ttcttgtccg atgtgttcct cacgaacacg
     8821 accagcatac atactacgtt tcattatttc tctcctcttt tattctgtta ctattttacc
     8881 ataaaagcgc aggctcttca tgaaaatcat cagaaaagtt tgccagtctt taaaagtcaa
     8941 gtgaaagccc taaaaattag cgctaatact cttcgaaaat ctcttcaaac cacgtcagcg
     9001 tcgccttacc gtaggtatgg ttactgactt cgtcagtttc atctacaacc tcaaaaccat
     9061 gttttgagct gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg
     9121 cggctagctt cctagtttac tctttgattt tcattgagta taatacaaaa atccgatgaa
     9181 cttcaccgga ctcttttatt ttgaattttt gcctgcttta cgcttttcag cgatttcggc
     9241 tgcctttcga ggcaagacaa tttccgttat gtaagccgtc ccaaaacgca gtacacctgc
     9301 aataggagca aagacaactg ctagatagtt atagaagaaa tcgcctttga aggcataagc
     9361 tagcgctcca atgatgaaaa atagaacgac tgcctgaatc actgctaata aaattactcg
     9421 tttcatgtga cctcctgact ctattatagc atgagaatca tcaaaaagcc gactaaatta
     9481 ttcaaagcgt gaagagaaat actgtagacc agaccttttc tgctaatgta agccaagccc
     9541 aaactaaaac caaggctaaa atagacaaaa aattgttgca catcacctgg aaaatgaatc
     9601 aaggcaaata gaagactaga taccagaaga aaaatcaggg ttcgtttact attgtcctgc
     9661 ttaggaaaga gatagcgtgc taacatccct ctaaaaacaa tctcttccgt caaaggagca
     9721 aaaataacca cagcaaagaa tgagaaaagt ggttgagaca aggtcaagtc tgtcgctatt
     9781 tgctgattta ctgaaggatc atctggcaag aagaattgaa cgaccagaga taagaaccaa
     9841 accaaggcag gaagccaaat aaatcggtta aagccgctct tctcaatatg aacaggagcc
     9901 ttctgatacc atttgtaaat gccgtacaca tatactccag ccaaggccac atagagtaga
     9961 gtaacagcat agggtgaagc gcctaaagca agcgacgcag tcgcgagccc ctgaataaag
    10021 ccatagataa ataaaaagga tagaagggct agaagaatcc agccaaggtt tttaagtaat
    10081 ttcatagata actcctttat ttgaaataac gttttaccat aggtaactgc atcacattga
    10141 tataaacatg gatggctcct acaagcaaga aagctagtaa ctgaatctct cctgtcaaga
    10201 aagaaatgat aataagaaaa atatataagg ctggtaagac atattggtgt aattggaata
    10261 aaattcgaaa actctgttcc aaattagcct gacgctcccc ttcatcataa gaatttatat
    10321 agttcaagac atcctttggt gtagcgaaaa attccaaatc aaactgacga acaatcgcaa
    10381 tggttttaaa aagagatttt tgagcgacta agaataccac aaagagtaaa aaagaaagga
    10441 aaaatgtttg agggtttgta tgcaatataa tcacctcact taatgaaata aaaatagcca
    10501 atggaatcgc tacacctgta atattaaaag caatggttcc aaactcaaga ttccgataca
    10561 tttgcacata ataggtttca ttcagatcgt catccatttc ctcttgatac aaggaatgaa
    10621 attttctgct tttctttaag aaattgaaag tcaaaaacat actaatgaaa cctatcagta
    10681 aacaaatagc tgatatccat ggcatcaagg cttttacatc taaaataatt tcgtgggatt
    10741 cgacacgtgc cttaaacatc cctacaaaca tgcccaagaa cccccccaag acaatagaca
    10801 tcaaaaataa caatctacgt ttctttttca tattcattct cctttttcac ttgctagatt
    10861 tttggatttc ttttcaatcc attcaattac tgggatgaga gcaaagtaga gccaaacaaa
    10921 ttggtcgcct tgatagggat taaaccagct taggtccatc ccaatcagta gaaatacgct
    10981 gactaataaa gctatgacca ctacataata aatcacttta tacttgttca tcactcgtcc
    11041 tcctccaaac gaaataccga ttcgactgtt tcgttgaaaa tttgagatat tttcagggca
    11101 atgataatgg atggggtgta ctcatcccgt tctagtaggc taatggtctg tctggaaacc
    11161 cctgccagtt tggctaggtc ggtttgattg agaccatcgc gagctcgaag ctcttttaga
    11221 cgatttttta gttgcacgtt acacacctac tctccgtcaa attcaacggt ttggatatct
    11281 tcaatacgtt gcaacttgaa tttttctttt cccgtattat caacactacg tagtttgacc
    11341 cattcctcat caacatccac aacttcccag ttatctggcc caatatacac tcccgttata
    11401 attggttcct ttccaatcat ttcttgtaat aatctcgaca tttctgcgtt tcctttctct
    11461 tttcgctcaa gttttttgat tttattctct agtttcttga tttttttaga attattagaa
    11521 taaaagaaaa tcataaatag tataaatcct agtacccaca ttataactcc tttctgcttc
    11581 ctatttctta acttgaattc attgtaacat atctttttct ttttgacaag tatatttgtc
    11641 aaaaagttta tgatttttgt cattttgcaa aagaaaaagg tcaggagtaa gttcctgacc
    11701 actttatcta tcattaatac tcttcgaaaa tctcttcaaa ccacgtcagc ttcaccttgc
    11761 cgtaggtatg gttactgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc
    11821 tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagcaacct gcggctagct
    11881 tcctagtttg ctctttgatt tttattgagt ataaaatcct agtttttcaa agatttctga
    11941 gaagttttgg ctgattgtct caagtgacac ttgcacttct tctcgagttt ggttgttctt
    12001 gaccgtcact tgtccgcttt cgacttcgct ctctcctagg gtgatgaggg tcttagccac
    12061 aaagacatcg gctgacttga actgagcttt tagtttacgg ttgaggtaat cacgctctgc
    12121 tttgaaacct tgttggcgaa gagcctgtac caattccaag gccttgatat ttgccccttc
    12181 gcccaagact gcgatataga catctagggc gttttcgata gggagggtca caccttgctt
    12241 ttcaagaatg agaagcaggc gctctacacc aagtccaaaa ccaaatccag cagtttcagg
    12301 gcctccaaaa taagaaacca aaccatcgta acgaccaccc gcacagacgg tcaggtcatt
    12361 gccctcaatc tctgtgataa actcgaaaat ggtgtggttg tagtagtcca gaccacgcac
    12421 catattggta tcgatgatgt aatctactcc aagattttcc aacatctgac gcacagcatc
    12481 aaaatgagct tggctttctt catcaagaaa gtccaagata gacggcgcat tctctactgc
    12541 ctccttgtct tctttttcct tagagtccaa gacacgaaga ggattttcct ccaagcgacg
    12601 ttggctatcc ttagacaagg tctccttgag cggtgtcaaa tagtcaatca aggcttggcg
    12661 gtaggctgca cggctctcag gatttccaag agtgttgagg tgcaatttga caccttgaat
    12721 accgatttcc ttcaaaaaat gggctgccat agcgattgtt tccacatcgg tagctggatt
    12781 gctagagcca aaacactcaa caccaatttg gtggaattgg cgcaagcgcc ctgcctgtgg
    12841 acgctcataa cggaacatag gtcccatata gtagaacttg cttggctttt gcacttctgg
    12901 ggcgaagagt ttattttcca cataggaacg gacaacgggc gcagttcctt ctggacggag
    12961 ggtaatatga cggtcaccct tgtcataaaa atcgtacatt tccttggtta cgatatccgt
    13021 tgtatctccg acagagcgac tgataacctc gtaatgctca aaaataggcg tgcgcacttc
    13081 tgcatagtta tagcgcttga aaatctcacg ggcaaagccc tcaacgtact gccacttagc
    13141 agattcagca ggtaaaatat cctgcgttcc ttttggtttt tgtaatttca tagggaatcc
    13201 tctttaaact taatagtctt attttaccac aaataaaggg attaaaacag taagaaaaaa
    13261 attaggattt agatatcatt tttgagatta agaattgtca aaaaaatagc tagcaaggaa
    13321 agaccaacaa atagcatcca agtcaactgt atattccata cggctactag tgaaaaacaa
    13381 gctgttccca caggtatgga taaggtaaac aatagaccta aaaaattact agtacgagct
    13441 agaacctctg gagctagatt tttcatgagc atggcactaa tctttggttg aactttacca
    13501 gacacataca gggtaaagaa gagaaatagc aaaccaagca cgacttgatt gaataaatta
    13561 gccaaaccaa ctagactaag tcctacggtc gcccacatca tcaatctagg caaggactgc
    13621 ttcccaaaat aatcattgcc cgtaaggcta ctgatgatga ttgctactaa aacacagaat
    13681 tgattgataa atagtgcctc tgtataagaa aaattcaaga gagaatggct caaaaagaag
    13741 atattataaa ttccacccaa agcgccaccc aaggaattaa taagcaagac agcaaagagc
    13801 ataaaaccaa agtttttctg cccactttta agaaaaacga gacgtaaatt tcggtaaatt
    13861 gttaggaact ggtctttgat agaaagcttc tcatttttta agttttcacc atcagcagat
    13921 gacattgaca ggctcaattt gctttttcct aaaaagagga tagtggctga tactaggaaa
    13981 aagcaggcat tgattcccgc aacgagagaa aaattgttga ccgatagagc taagagccag
    14041 actccgaaag cttgaccacc aatagctgaa atataggtga tgaactgtga aaaagaataa
    14101 gcctccatca aatcatcttc agctactttt tccttaataa gaggcatacg caggccacct
    14161 gcaaaatcac tgatgatatc actaatgaca ttgatcaaac acaggctaga aaaggcaaag
    14221 agactagctt gctgaacaac tagggctgct agaaaaaata gaaccgcctg aaacaaaccg
    14281 ctatagacca tccatttgac cttgtccctc gtgtaatctg cccgaatccc tacaaaaact
    14341 gtaaagaggg tcggaagaat catgacaata ttcgccatag caacagcaaa agatgcttgt
    14401 gacaaggtcg atgcatagac gataaagacc aggttgaaaa tcgaagcacc aaaagcattg
    14461 aagaagcgtg ataaaatcaa aattctataa gctggatttc taaacaatag tttcatagat
    14521 aatctccctt cttgttcgaa cctctatatg aagtatgact atagtatagc acttagaaat
    14581 ttgagaggga gaactcttga atatatgcaa caaataaaat gagcaaaaag aaacgataga
    14641 acttttgtaa tgacctgaga gagggcaata gttctatcgc ttcaaatttt ttcttggttt
    14701 gcagatattc aacattcggt cgtatttcta tagtattcgg cagatttatt acagccaagc
    14761 atctcaaaaa tacggacagc atcctccatc tttttctggc cttccttgac tctaccttgc
    14821 ttgctatcaa ggagaccttc tgcccacaga taaacaattc ggaaataggt ctcattttcc
    14881 ttgtagaaat gctcttcgat aacacgttta aaataatagg cattggtaaa ttcttcacac
    14941 tcaatactag ctaaaaagcc attcaatagt atagtatgaa aaaggtttcg attgccagac
    15001 atttccatta gaaaatcaga tttacgtacc atttctcgta catatctagt aaaaagagaa
    15061 acagataaaa atggagaact gactgaaaat aaattgagtt catagattcc ccagatctcg
    15121 gtagaaaaca aataatcatg aaggactttt ccttcctctg ctgttaactc taccctttca
    15181 tccatgctct tcatataaga cttgataata atggcattta gaatatgttt ctgtttgttg
    15241 tgagaatggg catgcttttg atactccctg cgatataagt cctcaagagg tgctatattc
    15301 tttggttcca agacatctgt aatttctttt ctcaactcag aatctgtatc atactggaaa
    15361 cctctcgcca gaaagaggat ctcctccaca ctggcagata tattttccag agcaaataga
    15421 aacttttcca ccgaaagctc actctgacct gtttcaaaac gggacaacat agacggcgaa
    15481 aattgtcctc cggttgcttg tctcagtgag atatttcttg actctcgtaa ttgtctaaag
    15541 acttttccaa tctgctccat agacttcccc ttgattccgt attttcttca ttttatccta
    15601 tttttcagaa aattcatcaa aaacttgcca aattgtcaga attatgagaa aatagaggat
    15661 atttatcacg tggagggact gctatgagag acgatatcaa aatcaatgac cgtgctttgg
    15721 ccttgcaaga ccaaattatc gaaaaactag agaaagtttt tgatacagat gtggaattgg
    15781 atgtttacaa tctaggtctg atttatgaaa tcaatctgga tgaaacgggg ctctgcaaga
    15841 ttgtcatgac cttcaccgat actgcctgtg attgcgccga aagcctgcct attgaaattg
    15901 tggcaggtct gaaacaaatc gagggtatca aagatatcaa ggttgaagtt acctggtcgc
    15961 ctgcttggaa aatcacacga atcagtcgct atggccgtat tgcccttgga ctaccacctc
    16021 gttaagcagg ccaatcactt ttaaagatga aaaaagcaag ccgaaaatgg cttgcttttt
    16081 gatactcaat gaaaatcaaa gggcaaacta gaaaactagc cgcaggttgc tcaaaacact
    16141 gttttgaagt tatggataga actgacgaag tcagctcaaa acactgtttt gaggttgtgg
    16201 atagaactga cgaagtcagc ccaaaacact gttttgaggt tgtggataga actgacgaag
    16261 tcagtaacca tacctacggc aaggcgacgt tgacgtgatt tgaagagatt ttcgagtatg
    16321 agtttatttt ttacctgact tgtccatatt ccagaagtct gtcacggctc cgcgtgaagc
    16381 agatgatacg atgtgggcat atttaccgag gacaccacgg ctgtaaagtg gtggcaaggt
    16441 tgtttctgcc ttgcgttttt caagttcttc ttcggatacg gccatggaaa tttctttggt
    16501 atcttggtca accgtaacga tatcgccggt acggagatag gcaattggtc caccatcctg
    16561 agcttcagga gcgatatgtc caacaaccag accataagta ccaccagaga aacgtccgtc
    16621 cgtcaagagg gccaccttat ctccctgacc tttaccaaca atcattgaag aaagtgacag
    16681 catctcaggc ataccaggac cacctttagg tccaacaaaa cgaacaacga ctacatcgcc
    16741 atcaacgatt tcatctgtca gaacggcctg aatcgcatct tcttctgagt caaagacctt
    16801 agctggccca acgtgacgac gcactttaac acctgatacc ttggcaactg caccgtcagg
    16861 agcaaggttc ccgttcaaga tgataagcgg accatccgca cgttttggat tttcaagtgg
    16921 catgataact ttttggcctg gagtcaagtc tgcaaagtca gccaagtttt cagctacagt
    16981 cttaccagta catgtgatgc gatctccgtg aaggaaacca tttgccaaca agtacttcat
    17041 aaccgcaggg acaccaccga cttcgtagag gtcttggaag acatactgac cagatggttt
    17101 caagtcggcc aagtgaggca cacgttcttg aatcgtattg aagtcctcaa gtgacaagtc
    17161 aacatttgcg gcatgggcaa tggcgagcaa gtgaagagtg gcgtttgtag aaccaccgag
    17221 agccatcgtt acagtaatag catcttcaaa ggcttcacga gtcaagatat ctgatggttt
    17281 gagaccaagt tccaacatct taacaacagc acgtcctgct gcttcgatat cttctttctt
    17341 atcagctgat tcagctgggt gagaggatga ccctggcaaa ctcatcccta gaacttcgat
    17401 agcagttgcc atggtattag cagtatacat accaccacag ccaccagggc cagggcaggc
    17461 attacattca agacgtttca cgtcctcagc tgtcatgtca ccgtggttcc attttccgat
    17521 accttcaaag acagaaacca agtcgatatc tttaccatca agatttcccg gtgcaatagt
    17581 tccaccatag gcgaaaatag ctgggatatc catattagca atagcaatca tagatccagg
    17641 catgttcttg tcacagccac cgatagcgac aaaggcatcc acgttgtgac cactcatagc
    17701 cgcctcgatg gagtccgcga tgatgtcacg agatgttaga gagaaacgca taccaggcgt
    17761 tcccatagcg atcccgtctg ctacggtaat ggttccaaac tgtacaggcc aagcgcctgc
    17821 agatttgaca ccttctttag ccagtttccc gaaatcatgc aagtgaatgt tacatggtgt
    17881 attttccgcc caagtcgaaa tcactcccac aatcggtgtt tcaaagtcct tatctgtcat
    17941 accagtcgca cgaagcatag cacggttagg tgatttaacc atgctgtcat aaatgctact
    18001 gcggtgacgt ttatctaatt cagtcatctt atccctccca tttcagtttt tactattata
    18061 gcacaatttt cgcatgaaga acagaataaa aatcttgaat tttcagaaaa ttctatacac
    18121 atgtgaaata tttaaaatta aaaacaacaa agcggattag tgcactttct gatgaccaga
    18181 atatgctttt taatccgctt tctttaaata acgtactgta atttttacag aaattctttc
    18241 agataagtgt atttaacatc tatcttgcat tataaatttc tagaaccttc tcttttatat
    18301 tcgattcact caaaccatac tcattaagaa gataatccat tttccctact tgaccgaatc
    18361 tttcttgaac acccatccga tgaatttttg tcattccatc atcagagaat aattcacata
    18421 aagaactgcc aattccacct atctgattgt ggttttctac agtaaatata gtttttccac
    18481 ttaacattgt ttttatctgt tctggtatcg gtttgattct aaataaatct atcacactta
    18541 ctgaataacc taatttagac agttcatctg caactcgaat acttggagca accattatac
    18601 cagaagcaac gattacaaga tcttcaccat gccttaactc aatgtagccc ttagaaaaat
    18661 cttctccacc ttgatacaca ggaactggag cttttctaat tgttcgaata tattttagtc
    18721 cttttaagtc taatgtctgg ttcaatattt cacgaaattg gatatcatca gttgcttcaa
    18781 aaatgatcga tttaggaatt aaacgtaaca atccaatttc ttcaaatggc atatgtgtcc
    18841 caccattcat ttctgcagtt actcctgcat ctgatccaat cacagtggca tccaattgtg
    18901 cgtatccaag agaaataaat aattgatcaa atactcttcg tgaagcaaaa ggaccaaatg
    18961 tatgaagata aggtctaaac ccctgaatag acaagcctgc tgcaaggccg accatttctg
    19021 cttccataat cccaacattc acataacggt ctccaaaatc cttttcaaga ttattagtag
    19081 ccatcgaact tgacaaatcg gcttctaaga ctactatatc agaataactt tgattagcct
    19141 ctaaaaggaa gtctctatat acatgccgta attctttcgt gcttctcatc attctgtttc
    19201 ctccaattcc tgacttaatc tttctacaac tgaagttaac atttgtctct cctctacagt
    19261 agggcgaaga tgatggttgg atttcatttc ttccagctct tgaacccctt gacctttaat
    19321 agtatctaat acaatgcact taggtgatga attatttgac tgttttaatt ggacaatccc
    19381 ttcataaatt tctctaatat ctgaaccctt gaccctaatg gattcaaatc caaatgctga
    19441 aaatttttct acgaaatcac ctggattaca aatatccttt gtaaaaccat ctaattgttt
    19501 tttgttatca tcaacaaata caattaagtt ggataactgt tgatgagaag caaactgtat
    19561 agcctcccaa cattgtccct catttaactc accatctcca acaatagcat aagtataaaa
    19621 gggactcttt cttattctct gaccatatgc aagtccagtt gcaacactaa ttccttgtcc
    19681 taaagagccc gttgtcatat ctatgcctgg cgttagattt ctatcaggat gagacggtaa
    19741 tttggttcca tttgtattta aagaatataa gaattctttg tcaaagaaac cattcaaata
    19801 gagtgtactg tatagagctg gtcctccgtg accttttgat aatatgaaat aatctctatc
    19861 tcgtgctgca aatatttctg gagtcattgg cattatttca ccataaagca ctgctaaaac
    19921 ttctacgata gacagacttc ctccgtaatg tccgaatcca agatgattca atgttctaag
    19981 agtatttaat cggatgttag tcgcaaattt tcttaactca tcttccctat ttttacttaa
    20041 aatcatccct tattcctcct ttgcagatgg ctttgtaata aaggatactc caaacataac
    20101 tgctagaata agaacaagac caatcacaat gcctgcttgt gagccaaatt gatttaacat
    20161 tcctaaaata attcctgata gaccaaaatc tgcatctgag aaagttgatc cttggaaacc
    20221 aagtcctccc aaaactggca ttaaaaagac tggaagaaaa ctgattaaaa taccttgtaa
    20281 aaatgctcca atagtggctc cacgaacacc accagatgca ttcccaatga cacctgcagt
    20341 cgctccacag aagaaatgag gcacaacacc tggtaagata acaaccgttc ctgaagcaat
    20401 cataattgcc atacttacta aaccaccaac aaaactagag ataaatccaa ttagaactgc
    20461 attgggtgca taagtataaa caatcggaca atccaaagca ggttttgaat taggtacaag
    20521 acgctctgaa atacctttaa aggctggaac aatttcgccc aaaataaggc gaacacctgc
    20581 taaaataaca aatacccctg ctgcaaattg acctgctaat tgtaaagcat aaactagacc
    20641 acttgtacca ctactgattt ctttttctat atattctgac cctgcaaaga tagctacaat
    20701 aatgtaaata actgccatgg ataaagtgat actaacagta ctatcacgta aaaaagctaa
    20761 actctttgga aatttaatgt cctctgttga ttttgatttg tcaccgataa ggctaccagt
    20821 aaaaccactc aaccaatatc ccaaagaact gaaatgacct aaagccacct tgtcatttcc
    20881 agttaattga accatatatt tttgaacaaa tgctggggaa atactcataa taataccgag
    20941 tgctaatcct cctagtaaga taagaggcaa gctagtaaag ccagcaactg ataaaatgac
    21001 cgcaatcata catgccatat atagagtgtg gtgccctgtt aaaaaaatat atttaaatcg
    21061 agtaaaacga gcgattaaga tattgaacac catgcctgca aacataatca gtgcagtagc
    21121 tgagccatat gttgttaaag ctacagctac aattgcttca ttattcggca caacgccaga
    21181 taaatgaaaa gcatgctcaa acatggtacc aaatggattt aaagaatttt gtacaattcc
    21241 tgcaccacca gatacaacta agaaaccaac aaaggtctta attccacctt taataatatc
    21301 aggtaatttc ttcttctgaa gaactaatcc taaaattgca attaaagcta ctaaaatagc
    21361 tggtgtacta acaatatcca atatgaactt catcataacg ctagcctcct atataagtcc
    21421 tttttcttca caaagtttag taattaattc tcgtagttca tccatatcaa taatactatt
    21481 taagatacga acatctccaa gatgactagc tgaatcagct agatcacgac caacaatcca
    21541 aatatcagct gcatttggat ctgctccacc taaatcataa tgttcaactt ctacatccga
    21601 aacattcaaa tcactcaata cagattcaat attcatctgt accataaaac ttgaacctaa
    21661 tcctgaacca caagctgtac caatttttaa cattatctaa tcctcctgtt taattatcat
    21721 tttaatgtca tcatagtttt ttgatgatat taaagtttga acatgatttt tatctcttaa
    21781 aattgttgtt aaatgtgaca aagcctttaa atgactctca ttatcaatgg ctgcaataca
    21841 aatcaacaat cttacctctt gttctggatt atccaataaa taaatcggtt cttccaaaac
    21901 taacattgac attcctattt cattcacacc ttcatctggc cgagcgtgag gaattgctac
    21961 tcccttccct aaattaataa aaggcccaaa ctcttctact ttttgaatca ttgcctcagg
    22021 gtagttctca gttatcttat cttgatccaa aagcggttta gctgctaaac gaatcgcctc
    22081 cttccatcct aatttttgcg aactaacctg ataggtttct ttggtaatga gttgttctag
    22141 cactggtaca atttcctttc tatcattttt ttggtaaaga taattcttta acgccaatct
    22201 taattccaat tcttgtgtaa taattccata tcttttgaca atattcagga tttgttcaat
    22261 ctcaaaatcc ccatactcta aattcggaaa atcttttaac actagttcta ctagttgtat
    22321 tgcttgctct tcagtcatca taaccgaaac tagataattt ggcttttctg tctccacctt
    22381 tatggtagaa aaaaccatat catagtcact actagctttc acctgtaaat catcaatctt
    22441 tgaggttcct ataaactcaa tttgaggaaa taatgctaat agattctctt ttaacatcaa
    22501 tgaagaacta acaccattag gacaaatgat tgctgcttta tagcattttt gaggcaaagt
    22561 atctgctttc tttaaataac ctccgaaatg gataacaaaa tatgctgttt cactatcagg
    22621 tatgggattg tcaatagcgt ccatcaaggg catcaaagaa tctttgacta attcaaataa
    22681 atcaggataa tgttctttaa catgcaatac atattcattt gaactaggta ggccgaactt
    22741 taatctatag taagccggta taaggtggcg gcgaagattt tctctcaatc cttccctttg
    22801 tttaaaatgt aacaaagaaa tatcttccat tctacttata atagcctctg ttaattgatt
    22861 aaagtaaacc ggagcaacat ctacttcacc ttcaaagcaa cttgataata aaacggtgat
    22921 atagcgataa tcatcctcag aaaacaccgt atctataatt cccaaatcaa ccactgtatc
    22981 caataaaata gtcgttatat cttgaataac aggagatact aatgtctctg aaagacatac
    23041 tctttcaaca tccctttgat acctacacag aatgaatact aaaccgaaaa ggcaaacttt
    23101 taattgatta acaataggta ctagctgtag cttctcataa taatctttaa ctacctgatt
    23161 tatcaaatca taagttaatg aataccccca actggataaa acataatcca aaccccaaat
    23221 ccctatggag gattccagca actcactaac catttgaaaa gctaagcggt gcttattcca
    23281 ctctgaaccg tgtaaagtat aaccttttgc tctactgtac cctagctcca aatcattatc
    23341 taacataatc tttcttaatg attgaatatc agataaggtt gtattcttac ttactttcaa
    23401 aaagtcttgg taatgattat tcgatataaa atctaatcgg caaaaagtgt aaagatagat
    23461 taaagctaag cgagtcgact ttggtaaaac caattcatcc gacttaataa tatctgtcaa
    23521 agactgcttc gtacgatttg ataaactata gcgaccttgt tttttatcca gcactatccc
    23581 tttattagct agataaggca ctaaataatc tattccttct ttgacttcct ttataggtaa
    23641 gctcacctta acagataatt catataatga tagctcacaa tgatccatca aagtcatcaa
    23701 aataactagt gctctataat caaacatttc tcttcctttc taactacaat tatagtgtaa
    23761 aaa
//
LOCUS       DAVLYR010000013        23545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLYR010000013 DAVLYR010000000
VERSION     DAVLYR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.013"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            650..2803
                     /locus_tag="VYO63_000488"
     CDS             650..2803
                     /locus_tag="VYO63_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1671465.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2816..4165
                     /locus_tag="VYO63_000489"
     CDS             2816..4165
                     /locus_tag="VYO63_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1671466.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4335..5042
                     /locus_tag="VYO63_000490"
     CDS             4335..5042
                     /locus_tag="VYO63_000490"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1671467.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5044..5187
                     /locus_tag="VYO63_000491"
     CDS             5044..5187
                     /locus_tag="VYO63_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1671468.1"
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQ
                     GLK"
     gene            5244..8969
                     /locus_tag="VYO63_000492"
     CDS             5244..8969
                     /locus_tag="VYO63_000492"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1671469.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9062..10504
                     /locus_tag="VYO63_000493"
     CDS             9062..10504
                     /locus_tag="VYO63_000493"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1671470.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGVEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10723..11745
                     /locus_tag="VYO63_000494"
     CDS             10723..11745
                     /locus_tag="VYO63_000494"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1671471.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIAGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11742..12287
                     /gene="purN"
                     /locus_tag="VYO63_000495"
     CDS             11742..12287
                     /gene="purN"
                     /locus_tag="VYO63_000495"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1671472.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12371..12880
                     /locus_tag="VYO63_000496"
     CDS             12371..12880
                     /locus_tag="VYO63_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1671473.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12905..14452
                     /gene="purH"
                     /locus_tag="VYO63_000497"
     CDS             12905..14452
                     /gene="purH"
                     /locus_tag="VYO63_000497"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1671474.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14574..15836
                     /gene="purD"
                     /locus_tag="VYO63_000498"
     CDS             14574..15836
                     /gene="purD"
                     /locus_tag="VYO63_000498"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1671475.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16239..16727
                     /gene="purE"
                     /locus_tag="VYO63_000499"
     CDS             16239..16727
                     /gene="purE"
                     /locus_tag="VYO63_000499"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1671476.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16714..17805
                     /gene="purK"
                     /locus_tag="VYO63_000500"
     CDS             16714..17805
                     /gene="purK"
                     /locus_tag="VYO63_000500"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1671477.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17815..18042
                     /locus_tag="VYO63_000501"
     CDS             17815..18042
                     /locus_tag="VYO63_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1671478.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18105..19403
                     /gene="purB"
                     /locus_tag="VYO63_000502"
     CDS             18105..19403
                     /gene="purB"
                     /locus_tag="VYO63_000502"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1671479.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19458..23396)
                     /gene="strH"
                     /locus_tag="VYO63_000503"
     CDS             complement(19458..23396)
                     /gene="strH"
                     /locus_tag="VYO63_000503"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1671480.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLETLDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6424 a         4690 c         5630 g         6801 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tagcttagaa atagcaggga cattagaaat
      241 tgaagtaata aataggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg
      301 cctagggtga cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcaggact
      361 cttgttctgc ctattttttt atccaaaaag tgcagttggg agggagatag gctcatttgg
      421 gaaggaagtc cagtttttgt ttagtgattg gggtaagata gttgttatca gatgagttta
      481 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtata tatgtgactg
      541 acttcgtcag tcctatctac aacctcaaaa cagtgttttg agcagcctgc ggctagtttc
      601 ctagtttgct ctttgatttt cattgagtat tagggaaaag gagatgaata tgaaatttgg
      661 gaaacgtcac tatcgtccgc aagtggatca gatggactgc ggtgtagctt cattagccat
      721 ggtttttggc tactatggta gttattattt tttggctcac ttgcgagaat tggctaagac
      781 gaccatggat gggacgacgg ctttgggctt ggtcaaggtg gcagaggaga ttggttttga
      841 gacgcgagcc attaaggcgg atatgacgct ttttgacttg ccggatttga cttttccttt
      901 tgttgcccat gtgcttaagg aagggaaatt gctccactac tatgtggtga ctgggcagga
      961 taaggatagc attcatattg ccgatccaga tcccggggtg aagttgacta aactgccacg
     1021 tgagcgtttt gaggaagaat ggacaggagt gactcttttt atggcaccta gtccagacta
     1081 taagccttat aaggaacaaa aaaatggtct gctctctttt atccctatat tagtgaagca
     1141 gcgtggcttg attgccaata tcgttttggc aacactcttg gtaaccggga ttaacattgt
     1201 gggttcttat tatctgcagt ctatcattga tacctatgtg ccagatcaga tgcgttcgac
     1261 actagggatt atttctattg ggctagtcat cgtctacatc ctccagcaaa tcttgtctta
     1321 cgctcaggag tatctcttgc ttgttttggg gcaacgcttg tcgattgacg tgattttgtc
     1381 ctatatcaag catgtttttc acctccctat gtccttcttt gcgacacgca ggacagggga
     1441 gatcgtgtct cgttttacag atgctaacag tatcatcgat gcgctggctt cgaccatcct
     1501 ttcgattttc ctagatgtgt caacggttgt cattatttcc cttgttctat tttcacaaaa
     1561 taccaatctc tttttcatga ctttattggc gcttcctatc tacacagtga ttatctttgc
     1621 ctttatgaag ccgtttgaaa agatgaatcg ggataccatg gaagccaatg cggttctgtc
     1681 ttcttctatc attgaggaca tcaacggtat tgagactatc aagtccttga ccagtgaaag
     1741 tcagcgttac caaaaaattg acaaggaatt tgtggattat ctgaagaaat cctttaccta
     1801 tagtcgagca gagagtcagc aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt
     1861 cggcattctc tggatggggg ctgttctggt catggatggc aagatgagtt tggggcagtt
     1921 gattacctat aataccttgc tggtttactt taccaatcct ttggaaaata tcatcaatct
     1981 gcaaaccaag cttcagacag cgcaggttgc caataaccgt ctaaatgaag tgtatctagt
     2041 agcttctgag tttgaggaga agaaaacagt tgaggatttg agcttgatga agggagatat
     2101 gaccttcaag caggttcatt acaagtatgg ctatggtcga gacgtcttgt cggatatcaa
     2161 tttaaccgtt ccccaagggt ctaaggtggc ttttgtgggg atttcagggt caggtaagac
     2221 gactttggcc aagatgatgg ttaattttta cgacccaagt caaggggaga ttagtctggg
     2281 tggtgtcaat ctcaatcaga ttgataaaaa agccctgcgc cagtacatca actatctgcc
     2341 tcaacagccc tatgtcttta acggaacgat tttggagaat cttcttttgg gagccaagga
     2401 ggggacgaca caggaagata tcttacgggc ggtcgaattg gcagagattc gagaggatat
     2461 cgagcgcatg ccactgaatt atcagacaga attgacttcg gatggggcag ggatttcagg
     2521 tggtcaacgt cagagaatcg ctttggcgcg tgctctcttg acagatgcgc cggtcttgat
     2581 tttggatgag gcgactagca gtttggatat tttgacagag aagcggattg tcgataatct
     2641 catggctttg gacaagacct tgattttcat tgctcaccgc ttgactattg ctgagcggac
     2701 agagaaggta gttgtcttgg atcagggcaa gattgtcgaa gaaggaaagc atgctgattt
     2761 gcttgcacag ggtggctttt acgcccattt ggtcaatagc tagaaagagg agaggatgaa
     2821 accagaattt ttagaaagtg cggagtttta taatcgtcgt taccataatt tttccagtag
     2881 tgtgattgta cccatggccc ttctgcttgt gtttttactt ggctttgcaa ctgttgcaga
     2941 gaaggagatg agtttgtcca ctagagctac tgtcgaacct agtcgtatcc ttgcaaatat
     3001 ccagtcaact agcaacaatc gtattcttgt caatcatttg gaagaaaata agctggttaa
     3061 gaagggggat cttttggttc aataccaaga aggggcagag ggtgtccaag cggagtccta
     3121 tgccagtcag ttggacatgc taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag
     3181 cctgcaagaa ggggagaacc actttccaga ggaggataag tttggctacc aagccacctt
     3241 tcgcgactac atcagtcaag caggcagtct tagggctagt acatcgcaac aaaatgagac
     3301 catcgcgtcc cagaatgcag cagctagcca aacccaagcc gaaatcggca acctcatcag
     3361 tcaaacagag gctaaaattc gcgattacca gacagctaag tcagctattg aaacaggtgc
     3421 ttccttggcc ggtcagaatc tagcctactc tctttaccag tcctacaagt ctcagggcga
     3481 ggaaaatccc caaactaagg ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt
     3541 agaatctagt cttgctactt accgtgtcca gtatgcaggt tcaggtaccc agcaagccta
     3601 tgcgtcaggg ttaagcagtc aattggaatc ccttaaatcc caacacttgg caaaggttgg
     3661 tcaggaattg acccttctag cccagaaaat tttggaggca gagtcaggta agaaggtaca
     3721 gggaaatctt ttagacaagg ggaaagttac ggcgagtgag gatggggtgc ttcatcttaa
     3781 tcctgagacc agtgattcta gcatggttgc agaaggtgcc ctactagccc aactttatcc
     3841 atctttggaa agagaaggga aagccaaact cacagcttat ctaagttcaa aatatgtagc
     3901 aagaatcaag gtcggtgatt ctgttcgcta tactacgact catgatgccg ggaatcaact
     3961 tttcctagat tctactatta caagtattga tgcgacagct actaagactg agaaagggaa
     4021 tttctttaaa atcgaggcgg agactaatct aacttcggag caggctgaaa aacttaggta
     4081 cggggtggaa ggccgcttgc agatgattac gggcaagaaa agttacctac gttattattt
     4141 ggatcaattt ttgaacaaag agtaatgttc gtgtttttag agttaaataa tttttaaact
     4201 gtgagaaaga ttcttcttgc agttttttct ttacaatttt tgaaaaacat ctactattta
     4261 ttcggttaaa ttcttgtgtt ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa
     4321 aggcgaactt taaaatgtca aaacaattga tctattcggg aaaagctaaa gatatctata
     4381 caactgagga tgaaaatctt attatttcaa cttacaagga ccaggcgact gctttcaacg
     4441 gtgtcaagaa ggagcagatt gcaggtaagg gagtcttgaa taatcagatc tcatctttta
     4501 tttttgagaa attaaatgtg gctggtgtgg cgactcactt tgtggagaaa ctttcagaca
     4561 cggaacaact caataaaaag gttaagatta ttcctttgga agtcgtgctc cgcaactata
     4621 ctgctggttc cttttcaaaa cgttttggtg tggatgaggg aatcgccttg gagactccga
     4681 ttgtcgaatt ttactacaaa aatgatgatt tggatgatcc atttatcaat gatgagcatg
     4741 tgaaattcct acagattgcg ggtgaccagc agattgccta cttgaaggaa gaaacgcgtc
     4801 gtatcaatga actattgaaa gtctggtttg ctgagattgg gcttaaattg attgacttta
     4861 agctagagtt cggttttgac aaggatggca agattatctt ggcagacgaa ttttcaccag
     4921 ataactgccg cttgtgggac gctgatggca accacatgga taaggatgtt ttccgtagag
     4981 gattgggaga actaaccgac gtttatgaga ttgtttggga aaagttgcag gaattgaaat
     5041 aatctgtttg caacggaaaa ccttcgtctc tcaactaaaa ggactcaggc tgaaaaggtc
     5101 ccccagacct tttcactctg tagagaacta ggtgaactaa cagatgttta cgaaattgtc
     5161 tgggaaaagt tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag
     5221 ctgaaataaa ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt
     5281 ttcaggtcaa gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct
     5341 tgaaaagtat tcgtattgtg caagtatatg atgtatttga cttggctgag gacttgtttg
     5401 cacctgcaga gaagcacatt ttctctgagc agggaaccga ccatgtttta gatgaagtat
     5461 ctgtgcaggc ggatcttgct aactatgctt tctttgccat tgaaagtctg ccagggcagt
     5521 ttgaccagcg tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg
     5581 tgacagtcaa cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg
     5641 aagctgtcaa aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgacag
     5701 ggattgccaa gcaggagttt tcagagtcag acaagaccat tccaaaattg actttctttg
     5761 aaagctatac agcagaagac tttgctcgct acaaggccga gcaagggatg gccatggaag
     5821 tggatgattt gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga
     5881 ctgaactcaa ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga
     5941 cagagttgaa acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct
     6001 atgacaagta tattgccatg cgcgaggagt taggtcggtc tgaaaaacca caaaccttga
     6061 tggatatggc gactattttc ggtcgttatg agcgtgctaa cggacgattg gatgatatgg
     6121 aagtctctga cgaaatcaat gcctgctcag ttgaaattga agtggatgtt gatggtgtca
     6181 aggaaccttg gctcctcatg tttaaaaatg aaacccacaa ccatccaaca gaaattgagc
     6241 catttggtgg agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt
     6301 cctatgttta ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg
     6361 aaactcgtgc tgggaaattg ccacaacaag tcatttctaa aacagcagct catggttatt
     6421 cttcatatgg taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag
     6481 gctttgtagc taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg
     6541 ttgtccgtga aaaacctgaa gcaggtgatg tgatcatcct tctcggaggc aaaacaggtc
     6601 gtgatggtgt cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga
     6661 ctgctggtgc tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct
     6721 tccgtaatgg caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggcg
     6781 tctgtgtggc tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc
     6841 ttaaatacca gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg
     6901 cggtcgtggt tcgtcctgaa gatgtggata ccttcgttgc cgaatgtaac aaagaaaata
     6961 ttgatgctgt tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg
     7021 gtgagacaat cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg
     7081 tcgatgccaa agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg
     7141 aaacactgga atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag
     7201 gattacagac tatctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg
     7261 gtcgttacca actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg
     7321 tgactcatac tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc
     7381 cataccacgg tgctgcttat gcggttatcg aagcaactgc tcgtttggtg gctgctggtg
     7441 ccaactggtc taaggctcgt ttctcttacc aagaatattt cgagcggatg gacaagcaag
     7501 cagagcgttt cggtcagcca gtagctgctc ttctaggttc tattgaagca caaattcagc
     7561 ttggcttgcc atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg
     7621 ttccgccaac cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc
     7681 cagaatttaa agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag
     7741 agattgattt tgacttgatt aagaaaaatt ttgctcagtt tgaagcaagc caagctgacc
     7801 ataaagtgac gtctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg
     7861 ctacctttgg aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga
     7921 cagctcaatt aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaaga
     7981 ttggacaaac gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca
     8041 agcttgacag tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag
     8101 cgaaagaact agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa
     8161 aggttgaaaa acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt
     8221 cagctaaggc cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga
     8281 atgaagaagc tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata
     8341 ttctcttctt tgccggtgga ttctcggctg cggatgaacc agatggttca gctaagttta
     8401 tcgtcaatat cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg
     8461 gtggcttgat tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac
     8521 catacggaaa ctttgaagat gctaacagta ctagtccaac cctcttctac aatgatgcca
     8581 accaacacgt ggccaagatg gtggaaactc gtattgccaa taccaactca ccatggttgg
     8641 ctggtgtgca agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg
     8701 tcgtgacggc tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg
     8761 ttgactttaa cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg
     8821 ccatcgaagg aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac
     8881 gttatgagga tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat
     8941 cagcggttaa acatttcact ggaaaataag acttacagat tttctaatag atagtatcag
     9001 taatgtaaaa gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa
     9061 aatgacatac gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca
     9121 tccagatgct gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga
     9181 gggggcagga atcctctcca atgatcaagg acaactgaag cgccatcgtg acatggggct
     9241 tttatcagaa gttttcagaa atccagccaa tttggataaa ttgacaggag ctggtgcgat
     9301 tgggcatgtg cgttatgcga ctgctggcga agcttctgtt gataacatcc aacccttcct
     9361 cttccgtttt cacgatatgc agtttggttt ggctcataat ggaaatctga ccaatgcagc
     9421 ctctctcaag aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga
     9481 aatcttggct cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga
     9541 agcgctcagc cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat
     9601 tgcggctctt gaccctaatg gctttcgtcc gctttctatc gggaaaatgg ccaacggagc
     9661 ggtggttgtt tcctctgaaa cctgtgcttt tgaggtcatt ggtgtcgagt ggatttgtga
     9721 tttgaagcca ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac
     9781 agatgatacc cagttggcga tttgttctat ggagtatatc tactttgccc gccctgattc
     9841 taatatccac ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg
     9901 agaatttaag catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc
     9961 tatgggattt gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata
    10021 cacccagcga acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa
    10081 actgtctgct gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat
    10141 tgtacgtgga acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga
    10201 ggttcacgtt gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat
    10261 ccagacccgt caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat
    10321 tggtgcggac agtctgactt atctttcaat tgatggcttg attgagtcga ttggtatcga
    10381 aacagatgcg ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc
    10441 tctctatgac tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa
    10501 gtaggcgaca gattctccat taaagaaaag gaaaaaacaa atgacaaata aaaatgcata
    10561 tgcctcacgt ctcactactg actaaaggct taagcattta gtcagtagac gctttgtcct
    10621 ataggatcaa agctagagcc ctgactagta tttttagata aaaagatggt ttatctaaaa
    10681 atacgtcgca gtctttctca aaaaaagaaa aggaaaaata aaatggcaaa taaaaatgcg
    10741 tacgctcaat ctggtgtgga tgttgaagcg ggttatgaag ttgttgaacg gattaaaaag
    10801 cacgtggctc gtacggagcg tgcaggtgtc atgggagctc ttggtggctt tggtggtatg
    10861 tttgaccttt ccaagactgg ggttaaagaa cccgtcttga tttcagggac tgacggtgtc
    10921 ggaaccaagc tcatgttggc tatcaagtac gacaagcacg ataccatcgg gcaggactgt
    10981 gtggccatgt gtgtcaacga catcattgct gcaggtgcgg aacccctcta ttttctcgac
    11041 tacgtagcga cagggaagaa tgaaccagct aagctagaac aagtggttgc tggtgtggca
    11101 gaaggttgtg tgcaggctgg tgctgccctc atcgctgggg aaacggctga aatgccgggc
    11161 atgtacggcg aagacgacta tgacttggct ggttttgcgg tcggtgtggc tgaaaaatct
    11221 caaatcattg acggttcaaa ggtggtagag ggagatgttc ttctcggact tgcttcaagt
    11281 gggattcact caaatggtta ctctttggtt cgtcgtgtct ttgcggatta cacaggtgag
    11341 gaagtcctac cagaattgga aggcaagaaa cttaaggaag ttctacttga gccgactcgt
    11401 atctatgtca aggctgtctt gccgctcatc aaagaagagt tggtcaacgg cattgcccac
    11461 atcacaggtg gtggctttat cgaaaatgtc cctcgtatgt ttgcagatga cctagctgct
    11521 gaaattgatg aaagcaaagt tccagtgctt ccaattttca aagcccttga aaaatatggt
    11581 cagattaaac acgaagaaat gtttgaaatc ttcaatatgg gtgtgggact tatgttggcg
    11641 gtcagccctg aaaatgtaga gcgtgtaaaa gaattgttgg atgaagcagt ctatgaaatt
    11701 ggtcgcatcg tcaagaaaga aaacgaaagt gtcattatca aatgaaaaaa atagcggttt
    11761 ttgcctctgg taatggctca aattttcagg tgattgccga agaatttcca gtggagtttg
    11821 tcttttcaga ccatcgtgat gcctatgtgc ttgagcgtgc aaagcagctc ggcgttctgt
    11881 cctatgcttt tgaactcaag gagtttgaga gcaagacaga ctacgaagca gcccttgtcg
    11941 aactcttgga agaacaccag attgacttgg tttgcctagc aggctacatg aaaatcgttg
    12001 gaccaacctt attgtcggct tatgaaggtc ggattgtcaa cattcatcca gcctacttgc
    12061 cagaatttcc aggagctcat gggattgagg atgcttggaa tgctggcgtg ggtcagtctg
    12121 gtgtgaccat tcactgggtg gattcgggtg tggatacagg ccaggtcatc aaacaggttc
    12181 gtgtgccacg actagctgat gataccattg acagatttga agctcgcatc catgaagcag
    12241 agtacaggtt gtatccggaa gtagtgaagg ctctatttac agattgactt tttgatgatt
    12301 catatgatat ctttgatttt aaattggagt cagtgtttgt tgaagacggc ttcaaacgga
    12361 ggtatttgta atgttagaat ctaaaaaaac aactcgatat gtattttatg tctatctgat
    12421 gttattaact tggggaatct tatttaagtt tgaaacaaat cctgaattta tagcattttt
    12481 cttagctcca aggtatatca attggattcc attttcagaa ccactaatag tcgatggaaa
    12541 aattgttttt gctgaaatgt tatttaatct gatttccttt attccattag gtgtttgttt
    12601 ccctttgata aaaactaatt tatctagttt aagaatagtc gggacaggtt tcttgattag
    12661 tttattgttt gagtgcttac agtatatttt agcaataggt ataacagata taacggattt
    12721 gactttaaat acgctaggtg tctgtgtagg cttactgatt tatcaaattt ttataagagt
    12781 gttcaaatca cagactagaa aatggatcaa tatcttaggt atgcttagcc ttggttttgc
    12841 ttatcttgtt ttactgttac tgcatttaat tggtgtttaa ctaatgatta aaaaggagaa
    12901 tataatgact aaacgcgtct taatcagcgt ctcagacaaa gcgggcattg ttgaatttgc
    12961 ccaagaacta aaaaaacttg gttgggagat tatctcgaca ggtggaacta aggttgccct
    13021 tgataatgct ggggtggaga ccattgcgat cgatgatgtg acgggcttcc cagagatgat
    13081 ggatggccgt gttaagactc ttcatccaaa tatccacggg ggtcttctcg ctcgtcgtga
    13141 tttggatagc cacttggaag cagccaatga gaaccaaatt gagcttattg accttgtagt
    13201 ggtcaacctt tacccattta aggaaactat ccttaaacca gatgtgactt atgctgatgc
    13261 agttgaaaat atcgatattg gtgggccgtc tatgcttcgt tcagcagcga aaaatcatgc
    13321 cagtgttaca gttgtggtag atcctgctga ctacgctgtg gttttggacg aattggcagc
    13381 aaacggcgaa acctcttatg aaactcgcca acgtttagca gccaaagtat ttcgtcacac
    13441 agcggcttat gacgccttga ttgcagaata cttcacagct caagtgggtg aaagcaagcc
    13501 tgaaaaactc actttgactt atgacctcaa gcaaccaatg cgttacggtg agaatcctca
    13561 acaagacgcg gacttttacc agaaagcttt gcctacagat tactctattg cttcagccaa
    13621 acagctcaac gggaaagaat tgtcatttaa taatatccgt gatgcagatg ctgctatccg
    13681 tatcatccgt gacttcaaag atagtccaac cgttgtggct ctcaagcata tgaatccatg
    13741 tggtatcggc caggcggatg acatcgagac ggcttgggat tatgcttatg agtctgaccc
    13801 agtgtctatc tttggtggta tcgttgttct taaccgtgag gtggatgctg cgacagctga
    13861 gaagatgcac ggcgttttcc tcgaaatcat cattgcacca agctatacgg atgaagcgct
    13921 agctattttg atcaataaaa agaaaaactt gcgtatcctt gccttgccat ttaatgctca
    13981 agaggctagc gaagtggaag cagaatacac aggtgtagtc ggtggacttc tcgtgcaaaa
    14041 tcaagacgtg gtcaaggaaa gcccagctga ctggcaagtg gtgaccaaac gtcagccaac
    14101 cgagacagaa gcgactgctc ttgagttcgc ttggaaggct atcaagtacg tcaaatcaaa
    14161 tggtattatc gtgaccaacg atcacatgac acttggtgtt ggtccaggtc aaaccaaccg
    14221 tgtggcttct gttcgccttg ccattgacca agccaaagat cgtctggacg gggcggtcct
    14281 tgcttcagat gccttcttcc catttgcgga taacgtggaa gaaatcgcca aagcaggaat
    14341 taaggccatc atccagcccg gtggctctgt ccgtgaccaa gaatccatcg aagcagcgga
    14401 taaatatggc ttgactatgg tctttacagg tgtgagacat tttagacatt aagaagataa
    14461 aagggaagaa aacagtttct ttcctttttt ggcttaaaat gctaactgaa acaagattaa
    14521 aacgaacttt tttgatataa tgttggtaaa taattcgcaa aagaggttga ggaatgaaac
    14581 tgcttgttgt cggttctggt ggtcgtgagc atgcgattgc taaaaagtta cttgaatcaa
    14641 aagacgtgga aaaagtcttt gtagctcctg ggaatgatgg gatgactctg gatggtttgg
    14701 aattggtaaa tatctctatt tccgaacatt ctaaattgat tgacttcgca aagaccaatg
    14761 atgttgcttg gacctttatc ggtccagatg atgcccttgc tgctggtatc gtggatgatt
    14821 ttaaccaagc tggacttaag gcctttggtc cgactagggc tgcagcggag ctggagtggt
    14881 ccaaggattt cgccaaggaa atcatggtca aatacggcgt tccgacagca acatatggca
    14941 cattttcaga tttcgaggaa gccaaggcct atatcgaaaa gcatggtgcg cctatcgtag
    15001 tcaaggcgga tggcttggca cttgggaagg gtgtcgtcgt tgctgagacg gttgagcaag
    15061 cggtcgaagc cgctcatgag atgcttttgg acaataaatt tggtgactca ggtgcgcgcg
    15121 tggttattga ggaattcctt gaaggagagg aattttcact ctttgccttt gtcaatggtg
    15181 ataagttcta catcatgcca acggctcagg accacaaacg tgcctatgat ggcgacaaag
    15241 ggcctaacac gggtggtatg ggtgcctatg cgccagtccc acacttacca cagagtgtag
    15301 ttgatacagc ggttgacacc attgtcaagc cagttctaga aggggtgatt aaagaaggtc
    15361 gcccttatct gggagttctt tacgcagggc ttatcctgac agctgatgga ccgaaagtca
    15421 ttgagttcaa cgctcggttc ggagatccag aaactcagat tatcttgcct cgcttgacct
    15481 ctgactttgc tcaaaatatc acagatatcc tggatagcaa ggagccaaat atcatgtgga
    15541 cggacaaggg tgtgactctg ggtgtggttg tcgcatccaa gggctacccg ctagactatg
    15601 aaaggggcgt tgagttgcca gcaaagacag aaggcgatgt catcacctac tatgcagggg
    15661 ctaagtttgc ggaaaatagc agagcactgc tctcaaacgg cggacgagtt tatatgctcg
    15721 ttaccacagc agataccgtc aaagaagccc aagccagcat ctaccaagaa ctatcccaac
    15781 aaaaaataga aggactcttc taccgaacag atatcggaag caaggcaatt aagtaaagat
    15841 ataagaataa cgcgccgtag tcgccaaaca cgataatggt cgtcgtggtg aaaagaccag
    15901 aacagtgaat gttctggtca gggggaaact tggagacctt aggctcaaag tttaggaatg
    15961 aaaccgaagg tttgcttccg cctccatcac ctaagaccat tatcaaaaag aaaaataaaa
    16021 attcacaaaa tacgttaatg atcgtatggt ttgcgagcgt tagcgagcta atatagaaca
    16081 atcaccgccg ttgtgaaaga acgattggat gataatccaa tcgttcaggg aaattggaag
    16141 accttgggtt tccaatttag gcatgagaca cctttggtgg ctgctgccgt ccctcacaag
    16201 ctaaggtgat tgttgaaaaa gaggaaaaag gagaagaaat gaaaccagta atttccatca
    16261 tcatgggctc aaaatccgac tgggcaacca tgcaaaaaac agcagaagtc ctagaccgct
    16321 tcggtgtagc ctacgaaaag aaagttgttt ccgcacaccg tacaccagac ctcatgttca
    16381 aacatgcaga agaagcccgt agtcgtggca tcaagatcat catcgcaggt gctggtggcg
    16441 cagcgcattt gccaggcatg gtagctgcca aaacaaccct tccagtcatt ggtgtgccag
    16501 tcaagtctcg tgctcttagt ggagtggatt cactctattc tatcgttcag atgccgggtg
    16561 gggtgcctgt tgcgaccatg gctatcggtg aagctggagc gactaacgca gctctctttg
    16621 ccctccgtct cctctctgta gaagataagt ccattgcgga tgcacttgcc aactttgctg
    16681 aagaacaagg aaaaatcgca gaggagtcgt caaatgagct catctaaaac aatcggaatt
    16741 atcggtggcg gtcaactggg tcagatgatg gccatttctg ctatctacat gggccacaag
    16801 gttatcgcgc tggatcctgc ggcggattgc ccggcctctc gcgtggcgga aatcattgtg
    16861 gcaccttata acgatgtgga cgccctccgt cagttggcag accgttgcga tgtcctcact
    16921 tacgagtttg aaaatgtcga cgctgacggt ttggatgccg ttatcaagga tggacaactc
    16981 cctcaaggaa cagatctgct ccgcatttcg caaaatcgta tttttgaaaa ggactttttg
    17041 tcaaacaagg ctcaagtcac tgtggcaccc tacaaggtcg tgacttctag cctagacttg
    17101 gcagatatcg acttgtcgaa aaactatgtc ctcaagactg cgactggtgg ctacgatggt
    17161 catggacaaa aggttattcg ttcagaagca gacttggaag cagcctatgc gctagcagac
    17221 tcagcagact gcgtcttgga agaatttgtc aactttgacc ttgagatttc tgtcatcgtg
    17281 tcaggaaatg gcaaggaggt gacgtttttc ccagttcagg aaaatatcca ccgcaacaat
    17341 atcctgtcta agaccatcgt accagcccgc atttctgaaa gtctagcaga caaggctaaa
    17401 gctatggcag tgcgaatcgc agaacaactc aacttgtctg gaactctctg tgtggaaatg
    17461 tttgcgactg atgatgacgt tattgtcaat gaaatcgccc cacgaccaca taactctggg
    17521 cactattcta ttgaagcctg tgatttctct cagtttgaca cccatattct cggtgttctc
    17581 ggagcaccat taccagtcat caaactccat gcgccagccg ttatgcttaa tgtcctcggt
    17641 cagcatgtcg aggctgctga gcaatatgtg acagaaaatt caagcgccca cctccacatg
    17701 tatggtaaaa tagaagcgaa gcacaaccgc aagatgggac atgtgacctt gtttagtgat
    17761 gtaccggata gtgtggatga gcttggggaa gggattgatt tttaggacaa gtctatgata
    17821 caaattatcg ttaatacatt tattgaaaag gataagactg gagcagttgt tgaagtgttg
    17881 tatgccagtg ctgaccaaga taaggtacaa gctaaatatg aagaactagc tgcacaatac
    17941 cccgaaaatt atttagctat ctatgatgta ccgctggata cggatttgaa tacactagat
    18001 cattacccgt ctgtgtttat tgaaaaagag gagtttgagt agaaatcttg gtttacctag
    18061 atagcttatt cccaacagct taagaagaaa ggaaaaatta acacatgatc aaccgttact
    18121 ctcgccctga gatggcgaat atttggagtg aagaaaataa ataccgtgct tggcttgagg
    18181 tggaaatcct ctctgacgag gcatgggctg agttggggga aatccctaag gaagatgtgg
    18241 ctttgattcg caagaaggcg gactttgaca tcgaccgtat tttggaaatt gagcaggaga
    18301 cgcgccacga tgtggtggct ttcacgcgtg cggtttctga gactcttggt gaagagcgca
    18361 agtgggttca ctatgggtta acttctaccg acgtggtgga tactgcttat ggttacctct
    18421 acaagcaggc caacgacatc atccgtcgtg accttgaaaa cttcactaat atcatcgctg
    18481 acaaggccaa ggagcacaag ttcaccatca tgatggggcg tactcatggt gtgcacgctg
    18541 agccgacaac ctttggtctt aaattggcaa cttggtacag cgaaatgaaa cgcaatatcg
    18601 agcgtttcga gcatgcagct gctggtgtag aagctggtaa gatttctggt gcggttggga
    18661 actttgccaa tatcccacca tttgtagagg agtatgtctg cgataaactt ggcatccgtg
    18721 cccaagaaat ctctacacaa gtccttcctc gtgaccttca cgctgagtac tttgcggttc
    18781 ttgccagcat tgcgacttca atcgaacgta tggcgactga gattcgtggt ctacaaaaat
    18841 ctgagcaacg cgaagtagaa gagttctttg ctaaagggca aaaagggtct tcagcaatgc
    18901 ctcacaaacg caacccaatc ggttctgaaa atatgactgg tctggcgcgt gtcattcgtg
    18961 gtcacatgat tacggcttat gaaaacgtcg ctctctggca cgaacgcgat atttctcact
    19021 catcagctga gcgtatcatc acaccagata cgaccatttt gattgactac atgctcaacc
    19081 gttttggaaa tatcgtcaag aacttgacag tcttcccaga aaatatgatc cgaaacatga
    19141 actcgacttt tggtcttatc tttagccaac gggctatgtt gacattgatt gaaaaaggca
    19201 tgacccgtga gcaagcctat gacttggtgc aaccaaaaac agcctactct tgggacaacc
    19261 aagtagactt taaaccactt cttgaggcag attcagaagt aacatcacgt ctcactcaag
    19321 aagaaatcga tgaaatcttc aacccagttt attacaccaa acgagtggat gatatctttg
    19381 aacgtcttgg actaggtgat taattaaaaa ataaacagcg agcttcaatc tcgctgttta
    19441 ttttttatcg aaaagactta gtcttctttt cttttagtga gtccataggc tgctagtgtg
    19501 gacatgagtc ctgcgactac tagtcctgca gaatcgtgag ttcctgtttc aggaagtttt
    19561 ttctctgtta ccacaggagc tggatcttga ggaagagctt tgctttcctc agcaggagca
    19621 gttgatggag ctggttggct tgggatttct agttttggtt tttcttcagc aatagcggct
    19681 tgtccgtttt catcgcctac atgtgttacc atagttccga cttcgactat ttgagtaacg
    19741 gcttcctgtg ctacgacact atttacaagt gttttcactt ccttaccatc ggcagaagtg
    19801 ctcacagagt agaagttgct acgatgtcca ttgacgccct tagtaatgac ttgtgttttt
    19861 cctttgagta agagtggatt ttcacgagtc actgtggtaa atggaatttc ttctttttgg
    19921 atatccagtc taggttttac ctcagtagtt ggtgcaagac cactttcatc acccttgtga
    19981 gttacaggag cgccaacttc aaccacttgg tttataactt ctttggttac ctggctatca
    20041 aggactgttt ctgttgtttt tccattttca gtgagtacag agatgtaatg agttcgttca
    20101 cctttgactc ctgctgtgat aatattttcc tgaccagctg ggaggttagg attttctttc
    20161 ttgataactt caaatggaat ttcttcagtt cttgtgatga gttctggtct ggtttcaaca
    20221 ttggcagcca cttcattttc atctaggctt cctgaatgag ttgcagctgg tttgaggcct
    20281 tgaagagcgg cttttaggtt ggctacaagt gtgtcaagct cagcttgttt attacggttg
    20341 aggttgtaat ttagagctgt tttagctgcg tcaagggtct caagactttc tttactatat
    20401 ccttctaagt ttgtaggaat tttagctaat tcttcgcgga gagcattata attagcacgg
    20461 aagtagtctt tgttgtggtc tgcaaaggca gtcatgagtt caaagatttc ctcttccttg
    20521 tattcagcgc ttggtctatc tgcccagatt gaaagcatac ttccgactgt tggaagatct
    20581 acttcaggat atttggtaga agctagttga ttgaatggtg tttttccagt attctcaata
    20641 gctttcttga ggaaaccacc accatcttct ggtttttgac caagaatgta gtaccagtca
    20701 ccgttggtat tcaagaattt atagcctttg cttgctaggt attgaggtga tgcgaggtta
    20761 tatccccacc agcctttaga ccagtaagaa atcaagacat ctttgtcaaa ctgaacatcg
    20821 tccttgtctt catagtagaa gccatcgttg aaggccattg gttgaagccc tctttctttg
    20881 gccatagctg cgagggtgtt ggcatattcg gcaaatttgc catagagttg ataccacttg
    20941 aggtagtacc agccttgggc actagtcgca tcgttggcgt attcgtcagt accaaagttg
    21001 aaaatctttg ttttacctgc aaagaagtcc atgtatttac cgatgagggc ttttacaaag
    21061 ttcatcgctt cttcgttttt caagtccata gttgtttttg aaactttatc aaagtgggct
    21121 tgaggatttt taatacctaa tttttccatg gcaaccagca tagcatccat gtgacctgga
    21181 ctgttaatag ctgggatgag accgatgtcc ttagatttag cgtattcaat tagctctgtt
    21241 acttctgcct gtgttagtgc agtaccgttt ggatcgtcgt agtaagcttt agttccttcg
    21301 ataatagctt ttttaacgtc atcactagca taggtttttc cgttggcagt aatggtcata
    21361 tcatcgagta gaaagcgaag tccgtcattt cctagaagga gatggacatc agaatatccg
    21421 agctcactgg ccttgtctac gatgcgtttg agctggttca gagtaaagta tttgcgtcca
    21481 gcatcgattg agattacctt gtttttggca agtttttcaa cctcacgttt agcttcttct
    21541 tctttttgag cttcaggcgt gagggtcaag ttgttgacag tttcttgaag tttagcaatg
    21601 gcttgatcaa tcgtatcttg ttgggcacgg ctaaggttgc tatcgagaga gcgaatagct
    21661 ttttcagctt cttttacggc cgtgacgctt tctgcagtat aacggttcag gtcttttggt
    21721 acctcgttaa gtgcttgctc tgcagattca taatcagctg cgaagtattc agcgttggca
    21781 tttgcaaaat gacgcatgag tttgaagagg cgtgatggtg aataacgtgc agatggagtg
    21841 tcagcccaag cagctaccat accaccgatg attgggatat cagctccttc tgtttttggt
    21901 acagaagtga ttggtgtgtt tttaatacca ttgagcccct gatcgagatt gtaccagcct
    21961 tggccatcag cgtttcgtcc aagaacgtag taccaagcat cattggtatt aaggatttgg
    22021 tgaccttttt cagctagtag tttagaagaa gcgacatcgt agcctcccca accaccagtc
    22081 cacatagaaa cgatgatgtc tttgtcaaaa ctaccaaagc ttgtgtcgct attgtagtag
    22141 ataccgtcgt taaaggccat tggtttgaga ccgtgcgatt ttacaatacg agcgaggtca
    22201 ttggcgtagg caataaattt ttcatagcct tttacagggt agccttcgtt tggatagtat
    22261 ttatcagctt gaagcacact ccaaccttta gcatctgtcg catcattggc atattcatca
    22321 agtccgatgt tgaagatttc agtctttttc gcgaaataag cagcatactt gtcgataagg
    22381 gcttttgtaa aagcgacagc ttgttcgttg tcaagatcga cagtacgggc tgatttcttc
    22441 ccaaaatagc taaagttagg gttttggatt cccaattctt tcatggcatt gagaatcgca
    22501 tccatgtgtc caggactatt tactgtcgga atgagaccga tacctttatc tttggcatag
    22561 ttaatcagat ctgtcatttg actttctgtt aagtgattgc cgtttggatc gttgtaataa
    22621 tcatttgtac ctttttcaat ggcgcgtttg acatcgtcac tggcataggt cttgccgtta
    22681 gctgtgatgc tcatatcgtc caacatgaaa cggagtccat catttccgac taataggtgt
    22741 aaatcagtgt agccataatg tttcgcttta tcgatgattt ccttgagctg ttctggtgag
    22801 aaatatttac gtccagcatc aatagaaaca attttctttt tcgctagttt ttcatttaca
    22861 gttgcagcac gttcctttcc tgcctctgtt tccggtttgt cagcctctgc tttcgcttca
    22921 tcttttttag ctggtttatc cttgtcagtc ttgtctgtat ttgactcttt agaatcaacc
    22981 tctttcgctt cttccttttt agggctagct tcttctgcct ttttattagc agtttctttt
    23041 tcagcagaag ttggagttac cacttctgct ttatcactag gagttgaact aacttcctct
    23101 tgtggttttt cttctgtttt tggaagacta gctaccttat cagtagctgg agtttctgtt
    23161 tctacagttt ttggagcttc tggttgaagc tctgctttag gtgtttcctc agtccgattt
    23221 tcggatgatt gaggggaatc agaaaccgta tggatggtcg gttggttttc tgtagtagta
    23281 ggagtaactc catcggctgc aacagtctgt gcttggaagg caaatccaat tagaacagaa
    23341 gctgctccta cagcgtattt acgaatagaa aaacgctgtt gtttttcatg tttcattgca
    23401 aaacctcctg attgcattgt tatattgata gcgattatat aaatcaacgc ctttatttta
    23461 tttcttatat taacgagagt caagaggaga tgacaaaaaa ctataataag tataaaaaaa
    23521 tataaaattt aaacttaaga tttca
//
LOCUS       DAVLYR010000014        21925 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLYR010000014 DAVLYR010000000
VERSION     DAVLYR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.014"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            36..1277
                     /locus_tag="VYO63_000504"
     CDS             36..1277
                     /locus_tag="VYO63_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1671481.1"
                     /translation="MKNIKENPELFVGIELEYPVASLEGDATDVEVIKDLFHYLVSTL
                     DLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVENRFNNYMNVI
                     QRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYG
                     TFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEES
                     MHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATPEIQ
                     AFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGL
                     LVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIEFSKDLLLLAEEG
                     LVVRNKEEMTYLQPLREELSL"
     gene            1353..2837
                     /locus_tag="VYO63_000505"
     CDS             1353..2837
                     /locus_tag="VYO63_000505"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1671482.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIALLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNMQGQYKLTDFDAEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQAAVEAYLGL"
     gene            2898..4178
                     /locus_tag="VYO63_000506"
     CDS             2898..4178
                     /locus_tag="VYO63_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1671483.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4182..4802
                     /locus_tag="VYO63_000507"
     CDS             4182..4802
                     /locus_tag="VYO63_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1671484.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            4968..6110
                     /locus_tag="VYO63_000508"
     CDS             4968..6110
                     /locus_tag="VYO63_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1671485.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPGEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6516..6854
                     /locus_tag="VYO63_000509"
     CDS             6516..6854
                     /locus_tag="VYO63_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1671486.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            6865..7223
                     /locus_tag="VYO63_000510"
                     /pseudo
     CDS             6865..7223
                     /locus_tag="VYO63_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
     gene            7330..7947
                     /gene="pcp"
                     /locus_tag="VYO63_000511"
                     /pseudo
     CDS             7330..7947
                     /gene="pcp"
                     /locus_tag="VYO63_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(7975..8832)
                     /locus_tag="VYO63_000512"
     CDS             complement(7975..8832)
                     /locus_tag="VYO63_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1671487.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVFFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            8962..10104
                     /gene="tgt"
                     /locus_tag="VYO63_000513"
     CDS             8962..10104
                     /gene="tgt"
                     /locus_tag="VYO63_000513"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1671488.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10202..12019
                     /locus_tag="VYO63_000514"
     CDS             10202..12019
                     /locus_tag="VYO63_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1671489.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTV
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12172..13323
                     /gene="nagA"
                     /locus_tag="VYO63_000515"
     CDS             12172..13323
                     /gene="nagA"
                     /locus_tag="VYO63_000515"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1671490.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            13486..14544
                     /locus_tag="VYO63_000516"
     CDS             13486..14544
                     /locus_tag="VYO63_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1671491.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            14695..15060
                     /locus_tag="VYO63_000517"
     CDS             14695..15060
                     /locus_tag="VYO63_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1671492.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15136..16077
                     /locus_tag="VYO63_000518"
     CDS             15136..16077
                     /locus_tag="VYO63_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1671493.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16025..17041
                     /locus_tag="VYO63_000519"
     CDS             16025..17041
                     /locus_tag="VYO63_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1671494.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17043..17369
                     /gene="comGC"
                     /locus_tag="VYO63_000520"
     CDS             17043..17369
                     /gene="comGC"
                     /locus_tag="VYO63_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV1671495.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNGGA
                     NRKVND"
     gene            17338..17766
                     /locus_tag="VYO63_000521"
     CDS             17338..17766
                     /locus_tag="VYO63_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1671496.1"
                     /translation="MEEQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLPVPKGIQAPSGQSIIF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            17771..18031
                     /locus_tag="VYO63_000522"
     CDS             17771..18031
                     /locus_tag="VYO63_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671497.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQININGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            17994..18455
                     /locus_tag="VYO63_000523"
     CDS             17994..18455
                     /locus_tag="VYO63_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1671498.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS"
     gene            18433..18846
                     /locus_tag="VYO63_000524"
     CDS             18433..18846
                     /locus_tag="VYO63_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1671499.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            18983..19570
                     /locus_tag="VYO63_000525"
     CDS             18983..19570
                     /locus_tag="VYO63_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1671500.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQAHLKAEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            19631..20584
                     /locus_tag="VYO63_000526"
     CDS             19631..20584
                     /locus_tag="VYO63_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1671501.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVELFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            20635..21825
                     /locus_tag="VYO63_000527"
     CDS             20635..21825
                     /locus_tag="VYO63_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1671502.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
BASE COUNT         6541 a         3856 c         5065 g         6463 t
ORIGIN      
        1 tgtcttgttc tatcgattta ttaaaacatc ggtatttgaa aaatattaaa gaaaatcctg
       61 aattgtttgt cggaattgag ttggagtatc ctgttgcaag tttagaaggg gatgctacag
      121 atgttgaagt tataaaggat ctatttcatt atttagtttc tactttggat ctcaccgtag
      181 caaaggtaga tgattttggc aatctgatcc agttagtaga tccgataagt caggatgcta
      241 ttttatttga agtttcctat acaacgattg agtttgcatt tggtaaggct gaaacgattc
      301 aagaggtcga aaatcgtttc aataattata tgaatgtaat tcagagaaag ttagctgaat
      361 caaatcatgc tattgttggc tgtggtatcc atcccaactg ggataaaaat gagaattgtc
      421 cagtggctta tccacgctat cagatgttga tggattattt gaatttgagt agaaatatta
      481 ttaaatcaga tttacatcat ttccctgaat atggtacttt tatctgtggg agccaggttc
      541 agctggatat ttcaaaaacc aactacttac gggtgattaa tgcttttact caaattgaag
      601 cggctaaggc ttatttattt gcaaactctg aattttcggg tgcggattgg gatacgaaaa
      661 tttcaaggga tattttctgg gaagaatcta tgcatggtat ctatccagag aatgttgggg
      721 tcaatgctag actccttaat gatgaagctg atttttttga ctatctaaat cattctgcga
      781 tttttactgc ggaacgtgat gggcagacct attattttta tcctattcag gctggggact
      841 atttggctac gcctgaaatc caagcatttg ctctgaatgg ggatgaggtt attatttccc
      901 cccaagagaa ggattttgaa actcatcgta gttaccagta ccaagactta acgactcgag
      961 gaacagttga gtttcgtagt gtgtgtacac agccacttga taggactttt gcttctgcag
     1021 cttttcactt gggattattg gttaatttag acaagttaga agcttactta gaaacagcac
     1081 ctttctttaa agtatttggt tatgattaca agtctttaag gagacaattt tctaagaaaa
     1141 atcttacaga tgaggaagaa actacgatta ttgaattttc caaagactta ctcctactag
     1201 ctgaggaggg actagtggtg agaaataagg aagaaatgac ctatttacag cctttgagag
     1261 aagaattgag cctataattt ctcttataaa gggagaattt tctgaaaaat catgatataa
     1321 tggacgagac tatagataaa ggatagagag taatgacatt agtttatcaa tcaacgcgtg
     1381 atgccaacaa tacagtaact gccagccaag caattttgca aggtttggcg acggacggcg
     1441 gtttgtttac accggttact tatccaaagg tagatttgga ctttgacaaa ttgaaagatg
     1501 cttcttacca ggaagttgct aagctagttt tgtcagcatt tttagatgac tttacagctg
     1561 aggagttgga ctactgtatc aacaatgcct acgatagcaa atttgatact ccagctattg
     1621 cactattagt gaaattagat gggcaataca atttggaact tttccatggt tcaacgattg
     1681 cctttaagga tatggccttg tctattttgc catactttat gacgactgct gctaagaaac
     1741 atggtttgga gaacaagatt gttatattga cagcgacatc tggtgacacg gggaaagctg
     1801 ctatggcggg gtttgcggat gtgcctggta ctgagattat cgtcttttat ccaaaggatg
     1861 gtgtcagcaa gattcaagag ttacaaatga ccactcagac tggcgacaat actcatgtta
     1921 ttgctattga tggtaacttt gacgatgcgc aaacaaatgt gaagcacatg tttaacgatg
     1981 tggctcttcg tgaaaaattg actaccaaca agttgcaatt ttcatcagct aactctatga
     2041 acattggtcg tctggtgcca caaattgttt attatgttta tgcttacgct caattggtta
     2101 agactggtga aattgtagct ggtgaaaagg ttaacttcac agtaccaaca ggaaactttg
     2161 gaaatatctt ggctgccttt tatgccaaac aaattggttt gccagttggt aaattgatct
     2221 gtgcttcaaa tgacaacaat gttttgacag acttctttaa aacacgtgtc tatgacaaaa
     2281 aacgtgagtt taaggtaaca accagcccat ctatggatat cttggtatct tcaaacttgg
     2341 agcgcttgat tttccatctt ttgggaaata atgctgaaaa gacagctgaa ctcatgaatg
     2401 ctttgaacat gcaaggacaa tataagttga cagactttga tgcagagatt ctggagctct
     2461 ttgcagctga atatgcgact gaggaagaaa cagcggcaga aatcaagcgt gtttatgagt
     2521 tagattctta tattgaggat ccacatacgg cggttgcctc agcagtttat agaaaatacc
     2581 aagcggttac tgatgatgcg actaagacag tgattgcttc aacagctagt ccctacaagt
     2641 tcccagtagt tgcagtagaa gctgtaactg gaaaagcagg tttaacagac tttgaagcct
     2701 tggctcaatt acatgaaatc tcaggcgttg cagtgccacc agcagttgat gggcttgaga
     2761 cagctcctgt tcgtcacaag acaactgtag cggctgctga tatgcaagca gcggttgagg
     2821 cttatttagg actttaagat agagggagta aactcggttg ggaaaccaac tgagtttctt
     2881 ttcatcagga ggagagattg tttaagaaaa ataaagacat tcttaatatt gcattgccag
     2941 ctatgggtga aaactttttg cagatgctaa tgggaatggt ggacagttat ttggttgctc
     3001 atttaggatt gatagctatt tcaggggttt cagtagctgg taatattatc accatttatc
     3061 aggcgatttt catcgctctg ggagctgcta tttccagtgt tatttcaaaa agcatagggc
     3121 agaaagacca gtcgaagttg gcctatcatg tgactgaggc gttgaagatt accttactat
     3181 taagtttcct tttaggattt ttgtccatct tcgctgggaa agagatgata ggacttttgg
     3241 ggacggagag ggatgtagct gagagtggtg gactgtatct atctttggta ggcggatcga
     3301 ttgttctctt aggtttaatg actagtctgg gagccttgat tcgtgcaacg cataatccac
     3361 gtctgcctct ctatgttagt tttttatcca atgccttgaa tattcttttt tcaagtctag
     3421 ctatttttgt tctggatatg gggatagctg gtgttgcttg ggggacaatt ctgtctcgtt
     3481 tggttggtct tgtgattttg tggtcacaat taaaactgcc ttatgggaag ccaacttttg
     3541 gtttagataa ggaactgttg accttggctt taccagcagc tggagagcga cttatgatga
     3601 gggctggaga tgtagtgatc attgccttgg tcgtttcttt tgggacggag gcagttgctg
     3661 ggaatgcaat cggagaagtc ttgacccagt ttaactatat gcctgccttt ggcgtcgcta
     3721 cggcaacggt catgctgttg gcccgagcag ttggagagga tgattggaaa agagttgcta
     3781 gtttgagtaa acaaaccttt tggctttctc tgttcctcat gttgcccctg tcctttagta
     3841 tatatgtctt gggtgtacca ttaactcatc tctatacgac tgattctcta gcggtggagg
     3901 ctagtgttct agtgacactg ttttcactac ttgggacccc tatgacgaca ggaacagtca
     3961 tctatacggc agtctggcag ggattaggaa atgcacgcct ccctttttat gcgacaagta
     4021 taggcatgtg gtgtatccgc attgggacag gatatctgat ggggattgtg cttggttggg
     4081 gcttgcctgg tatttgggca gggtctctct tggataatgg ttttcgctgg ttatttctac
     4141 gctatcgtta ccagcgctat atgagcttga aaggatagga aatgcaaaaa acagctttta
     4201 tttgggattt agacgggact ttattggact cttacgaagc gattttatca gggattgagg
     4261 agacttttgc tcagttttct attccttatg ataaggagaa ggtgagagag tttatcttca
     4321 agtattcggt gcaaaatttg cttgtgcggg tggcagaaga tagaaatctg gatgttgagg
     4381 tgctaaatca ggtgcgtgcc cagagtctgg ctgagaaaaa tgctcaggta gttttgatgc
     4441 caggtgcgcg tgaggtgcta gcttgggcag acgaatcagg aattcagcag tttatatata
     4501 ctcataaggg gaacaacgct tttaccattc tcaaggactt gggggtggaa tcctatttta
     4561 cagagatttt aaccagtcag agtggctttg tgcggaagcc aagtccagaa gcggctacct
     4621 atctgctaga taagtatcag ttgaattctg ataatactta ttatataggg gatcggactc
     4681 tggatgtgga atttgcccag aatagtggga ttcaaagtat caacttttta gagtctactt
     4741 atgaagggaa tcacaggatt caagcgttag cagatatttc ccgtattttt gagactaagt
     4801 gataaaaaga ttgtgtcagt tttgtgacag agacctaaca aactatttca agtaacctag
     4861 tttgttacaa ggaatagaca gttctgttaa ataggcccga gagggctttt tttctacatt
     4921 ttttgtgtta tgatagacag gtactaattt gaaaggaatt tgaaagaatg aagaaaagaa
     4981 tgttattagc gtcaacagta gccttgtcat ttgccccagt attggcaact caagcagaag
     5041 aagttctttg gactgcacgt agtgttgagc aaatccaaaa cgatttgact aaaacggaca
     5101 acaaaacaag ttataccgta cagtatggtg ataccttgag cactattgca gaagccttgg
     5161 gtgtagatgt cacagtgctt gcgaatctga acaaaatcac taatatggac ttgattttcc
     5221 cagaaactgt tttgacaacg actgtcaatg aagcagaaga agtaacagaa gttgaaatcc
     5281 aaacacctca agcagactct agtgaagaag tgacaactgc gacagcagat ttgaccacta
     5341 atcaagtgac cgttgatgat caaactgttc aggttgcaga cctttctcaa ccaattgcag
     5401 aagctccaaa agaagtagca tcaagttcag aagttacaaa gacagtgatt gcttctgaag
     5461 aagtggcacc atctacgggc acttctgtcc cagaggagca aacggccgaa acaagcagtg
     5521 cagttgcaga agaagctcct caggaaacga ctccaggtga gaagcaggaa acacaagcaa
     5581 gccctcaagc agcatcagca gtggaagcaa ctacaacaag ttcagaagca aaagaagtag
     5641 catcatcaaa tggagctaca gcagcagttt ctacttatca accagaagag acgaaaataa
     5701 tttcaacaac ttacgaggct ccagctgcgc ccgattatgc tggacttgca gtagcaaaat
     5761 ctgaaaatgc aggtcttcaa ccacaaacag ctgcctttaa agaagaaatt gctaacttgt
     5821 ttggcattac atcctttagt ggttatcgtc caggagacag tggagatcac ggaaaaggtt
     5881 tggctatcga ttttatggta ccagaaagtt cagaattagg ggataagatt gcggaatatg
     5941 ctattcaaaa catggccagc cgtggcatta gttacatcat ctggaaacaa cgtttctatg
     6001 ctccattcga tagcaaatat gggccagcta acacttggaa tccaatgcca gaccgtggta
     6061 gtgtgacaga aaatcactat gaccacgttc acgtttcaat gaatggataa acccgacttg
     6121 gtaacatcat tttgacgaat gagatctagc tttcgtgata ggaagcgatt ctcgttcgtt
     6181 ttttctttgt catactcttc gaaaatctct tcaaaccacg tcagttttat ctgaaacctc
     6241 aaagctgtgc tttgagcaac ctgcgactag ctttctagtt tactctttga ttttcattga
     6301 gtatcaattt gaatggaaaa tggaaagtta ttatcttgta ataatccaag caacattctt
     6361 gcaatctatt ttactttata tcacaattga ttggtcaact attgataagt caatggatag
     6421 gaggaagaaa tgatagagat tcaagattta ctgtatcaac tccgcttgtc tgagcaagcg
     6481 agtacgcaat tgtttgaaaa aggcttggga ttagtttgac acggtatcag attttactgt
     6541 ttttgctgga gcattctcct tgtaaccaaa tggcggttca ggagcgtttg aaaattgatc
     6601 aggctgcttt gacacggcat ttcaagattt tggaaacgga aggtttggtg gagcgtcatc
     6661 gtaatcctga aaatcagcgg gaagtgttgg tagaggctgc gaagtatgcc aaggagcagt
     6721 tagtggtgaa tccccctctg caacatatca aggttaagga agagatggaa agtatcttaa
     6781 cagagtttga gagaacagaa ctcagccgtt tattaaataa attggttttg ggtattgaaa
     6841 atatagaaat ttaaggagaa atagatgtca attattttaa caacgatcgt tgctttggag
     6901 catttttaca ttttttattt ggaaagtatt gccacgcaat cagatgcgac tagtcgtgta
     6961 tttaatatgg aaaaggaaga attggctcat ccgtcagtaa gttcattgtt caaaaatcaa
     7021 ggaatttata aggctctgct aggagtcttt ctcttgtatg tcatttattt ctcacagaat
     7081 ttagaaattg tgactatttt tgtcttattt gtgattggtg ctgcgactta cggctcttta
     7141 acagcggata aaaaattatt ttgaagcaag gtggatcagc tattttggcc ttgattagta
     7201 ttttactctt taaatacact tgaaggttga ttctaatctc gctaatcctt tttaatccag
     7261 aataagggaa atatgttata cttgttttta agaaaaaagt ctcattgaat tggttttgag
     7321 gagttagaaa tgaaagtatt agtgacaggt tttgagccct tttgaggcca ttaaaggttt
     7381 accagctgaa atccatggtg ctgaggtccg ttagctagag gtgccgacag tttttcacaa
     7441 atctgctcaa gtattggaag aagagatgaa tcgttatcaa cctgactttg tcctttgtat
     7501 tgggcaagct ggtggaagaa ctagtttgac acctgaacga gtggccatta atcaagacga
     7561 tgcacgcatt tctgataacg aagataatca accgattgac cgtcccattc gcccagatgg
     7621 tgcttcggcc tactttagta gtttgccgat taaagcgatg gttcaagcta taaaaaagag
     7681 ggcttaccgg cctctgtttc caatacggca gggacttttg tctgcagcca tttgatgtat
     7741 caggctctct atttggtaga aaagaaattt ccatatgtta aggcaggttt tatgcatatt
     7801 ccttatatga tggaacaggt ggtgaacaga cagactattc cagctatgag tttagtggat
     7861 attcggagag ggatagaagc agcaatcggc gctatgatag aacatggaga tcaggaactc
     7921 aagttggtag gcggagaaac tcattgatag aaaaaagctt gagtggaaaa accttcaagc
     7981 ttttggacgt tttcgagcca atactgctcg gtaaaacata attttagtgc attggatata
     8041 aggtaggagt gaaaaactag caatgccaaa ggtaatccaa ttgaggaagt accaaggaag
     8101 aagctgtaaa tctaggacaa agcgctggaa cttgtagccc ttcataaagg aacggctagt
     8161 ttttaggatt cgtcttggtg ggacctgtcc taggtctaga ctataacaga gaagaaattc
     8221 cacctgtgaa taggcataat actgtggaat atagaggata tttcctacaa tgatcaagat
     8281 gagacttgca agaaagtaga gtccaaagac catgaggaaa cgctcggttt caactgatga
     8341 gagatctaga tttggaaact caggatgtag ggtgacgaat tttttggcta aaaagctact
     8401 ataaaagagg aggtaaatcc caagtaaatt agggatactc cataaaaaga gatagaaacg
     8461 tttgagaagt agggtcaaaa aggtttgaga aaagcgctcc tcatcaaaga gagctaggct
     8521 gttttttaca gatggctccg ttttagaatc tttcatgagt gtcagtgttg catagacgga
     8581 actggtcaaa agaatagtcc cgataaagaa gactagtaga ggaaagaggt aggcttgaag
     8641 tatttggcca agtatgctga aaaatggctg ttctaaaaca gtcccgtgga tccgagataa
     8701 gggattaaga aaactagata agatgaccag catactggga aggatataga ggagaaagag
     8761 acggggggtg tcagcctgaa aatgttttga ctcctgacga attgttttta aatcaatttt
     8821 tggatagttc attctcttat tataccatag ttcttataca tagttcgtga cagttcctac
     8881 tttttttgat aaaatcatac agtgtgtcct tgggcacact gtatgaactg ggactgtctt
     8941 tcccagcttc ggaggtaaaa aatgtcagat tcaccaatca aatatcgttt gattaagaaa
     9001 gaaaaacaca caggagctcg tctgggagaa atcatcactc cccacggtac ctttccgaca
     9061 cctatgttta tgccagttgg gacacaagcc actgtcaaaa ctcagtcacc tgaagaattg
     9121 aaggagatgg gttcgggaat tatcctatca aacacctatc atctctggct tcgccctgga
     9181 gatgaactca ttgcacgcgc tggtggtctc cacaagttca tgaattggga ccagcctatc
     9241 ttgacagata gtggtggttt tcaggtttat tctttagcag atagccgtaa tatcacagaa
     9301 gaaggagtaa cctttaaaaa tcatctaaat ggttctaaga tgttcctatc cccagaaaaa
     9361 gccatctcta ttcagaataa tctgggttca gacatcatga tgtcctttga tgaatgtcct
     9421 cagttttatc aaccttatga ctacgttaag aaatcgatcg agcgtaccag ccgttgggct
     9481 gagcgtggtt tgaaggctca ccgtcgtcca catgaccaag gtttgtttgg aattgtgcaa
     9541 ggtgcaggat ttgaagacct tcgccgccaa tcagctcatg atcttgtcag catggatttc
     9601 tcaggctact ctatcggtgg tttggcagtg ggagaaaccc atgaagagat gaatgcggtc
     9661 ttggacttta caactcaact gctacctgaa aataaacctc gttatctgat gggtgtggga
     9721 gcgccagata gcttgatcga tggggtcatt cgtggggtgg atatgtttga ctgtgtctta
     9781 ccgactcgaa ttgctcgtaa cgggacttgt atgaccagtc aaggacgttt ggttgtgaaa
     9841 aatgcccagt ttgctgagga ctttacgcca ctggatcctg agtgtgactg ctacacatgt
     9901 aataactata cacgcgctta ccttcgtcac ctgctcaagg ctgatgaaac ctttggtatc
     9961 cgcttgacta gctaccacaa tctttacttc ttgcttaacc tgatgaagca agtgcgacaa
    10021 gccatcatgg atgacaatct cttggaattc cgtgagtatt ttgtggaaaa atatggctat
    10081 aataagtcag gacgtaattt ctaaaatgga attgatataa aaaaatccta agttttctct
    10141 taggattttt cttctttttt tgatagaata aagtgtacaa tgaaaggaag aataaactcg
    10201 tatgcgcatt aaatggtttt ccttgattag gattataggt ttacttttgg tactcttgta
    10261 tcacttcttt cagacgatct ttcctggagg attcttcggg gtagatgtct ttttcacatt
    10321 ttcaggtttc ctgattacag ctctactcat tgaagaattt tctaagaaca atgagattga
    10381 tttgatagga ttttttagaa gacgctttta tcggattgtg ccacctgtgg ttttgatggt
    10441 cttggtgacc atgcctttta ctttcttggt tcgccaagac tatgttgctg gaattggtgg
    10501 ccagattgcg ggcgtcttag gctttatgac caacttctat gaactcctaa caggtgggag
    10561 ttatgaatct cagttcattc ctcatttgtt tgttcataat tggagtttgg cagttgaggt
    10621 tcactactat attctttggg gattggcagt ttggttctta tccaaacaag ctaaatcaaa
    10681 tggtcagttg aaggggatgg tctttctctt atctgctgct gccttcttga tcagtttctt
    10741 ctccatgttt attggtagtt ttctagtgac ctcttattcc tctgtttatt tctccagttt
    10801 aactcatgtc tatccattct ttttgggaag tatgttagca actattgtag gcgttcgtca
    10861 gacgacttcc ctcgtcaagc agttggataa aatctgggat ttacgaaaga ctttggtagt
    10921 ttttggagga ggctttggtt tcctagttct tttgactttc tttgtcaaat tcacttatct
    10981 ttttgcctat cttatcggct tcttgcttgc cagtcttgca gctcttgcca tgattctggc
    11041 ggcgcgtgtc ttacatgaaa agacacatca tatacaggag ccgaagatta tcagcttttt
    11101 agcggatact agctatgcgg tttatctttt ccattggcct ttctatatca ttttctcaca
    11161 gttgacatca aatcttcttg ctgtattact gactctgatt tgttcttatg gctttgccag
    11221 tctgtcattc tatgtattgg aaccttggat tgcaggcaag aacacaccta ttttacaaac
    11281 tcttcgtccc ctgccttata ttcacgcaat tcttgcagca ggtacaggaa tcttgacctt
    11341 cattgtctgc atggtgacct tgttggcacc acaagtggga gcgtttgaga cagacttgac
    11401 tgtcaatggc ttgaagcaag ctgcaacaaa tattggccag accaaggtga tggcagaacg
    11461 ggcagatgca aacagtttgg gaattgctga tggcactatg ttaattggtg actcagtggc
    11521 tttaagggca aatacagcac tacagacagt tcttcctgga gcacagatta acgcgcaggt
    11581 cagcgtaaca accaagaccg caaatgaaat catgctaaat aatagccaga ataaattttt
    11641 acctaagacg gtggtcattg cgactggggt aaataatcct gagaattaca aggatgactg
    11701 ggacagtatc gtgaaaaatc ttcctaaggg acaccatatg attttggtga ctccttatga
    11761 gggagataag acaaaagaga cctatgccat cgttgagaag gctgctgcct atatgagaga
    11821 attggcagag aagacacctt acattacgat agcagattgg aatcaagttg cgaaagagca
    11881 tccagaaatt tgggcaggaa cagaccaggt tcatttcggg agtgagagta gcactatcga
    11941 agcaggagca aaattgtatg cagatacgat tgccacagct ttgcagacag ctcaagacaa
    12001 gccggttaaa tcaaaataac ttgtattaaa aaagagaaga gttggagaaa tccagctctt
    12061 ttaaaatatc tccaaaaaat aggtctatac catttacaaa taaaaaagaa aggtttataa
    12121 tgtaattgac ataataaatt gtagaatcaa tcttttaagg aggttaacat tatgcctaac
    12181 tatattaaag cggatcagtt tttctaccca cacggagttc gtcgaggtgg ttacttggaa
    12241 cttgtggacg gcaagtttgg gaaacatgta gagcagattc ctgaaggggc tgaggtgatt
    12301 gactatacag gttatagcat tgccccaggt cttgtggata ctcatattca tggatatgca
    12361 ggtgtagatg tgatggacaa caacattgaa ggtacattgc atactatgag tgaaggactt
    12421 cttagtaccg gtgttaccag tttcttaccc acaactttaa cagccactta tgagcaattg
    12481 cttgcagtca ctgaaaatct tggaaaccat tataaagaag caacaggtgc taagattcgt
    12541 gggatttatt atgaaggtcc atatttcaca gaaactttta agggggcaca aaatccaact
    12601 tatatgagag acccgggtgt tgaggagttt cattcttggc aaaaagcggc aaatggcttg
    12661 cttaataaaa ttgcccttgc accagaacgt gatggggtgg aagactttgt tcgtacagtt
    12721 acgggcgaag gtgtgacggt tgctcttgga cattcaaacg cgacttttga tgaagccaaa
    12781 aaagcagtcg atgctggagc gagtgtttgg gtgcatgcct acaatggaat gcgtgggttg
    12841 actcaccgtg aattgggtat ggttggagcc atgtaccaat tgccacatac ctatgcagag
    12901 ttgatctgtg atggtcacca cgtagatcca aaggcttgcg aaattcttat caaacaaaaa
    12961 ggaacagaaa acatcgctct tatcacagac tgtatgacag ctgggggatt ggaagacgga
    13021 gattatatgt tgggagaatt cccagttgtc gttgcaaatg gaactgcacg cctcaaatcg
    13081 acaggtaact tggcaggttc tatcctcaaa ctcaaagatg gtttgaagaa tgtggtcgaa
    13141 tggggaattg cgaatccgca tgaagcagtc atgatggcca gcttcaaccc agctaaatcc
    13201 gttcacatcg atgacgtctg tggccaaatc cgtgaaggct acgacgctga cttcatcgta
    13261 ttagataaag atttggaatt ggtagcaacc tacctagatg gcgtgaaacg ttatcaagca
    13321 taagagagaa agcagaagtt agagactaac ttctgctttt ttatgcaggg tactttactg
    13381 gtaaataaaa agttaaaaaa atcacaaaaa aagcttgaag aaacaaagaa aaaggcttac
    13441 aattgaacta taaatattac aaataaaaaa acggaggagt gctttatgaa agcttatact
    13501 tatgttaaac caggacttgc ttcttttgtt gatgtagaca aaccagttat tcgcaagcca
    13561 acagacgcta ttgtgcgtat tgtaaaaacc actatttgtg gaacagacct ccatattatc
    13621 aaaggggatg ttcctacttg ccaaagtggt accattcttg gccacgaagg gattgggatt
    13681 gttgaagaag ttggggaagg agtttccaac ttcaaaaaag gtgacaaggt cttgatttct
    13741 tgcgtctgtg cctgtggtaa atgctactac tgtaaaaaag gaatttatgc ccactgtgaa
    13801 gacgaagggg gctggatttt cggtcacttg attgatggta tgcaggctga atatctacgt
    13861 gtccctcatg cagataatac tctttaccat actccagaag acttgtcaga tgaagctttg
    13921 gttatgctgt cagacattct gcctactgga tatgaaattg gtgtcttaaa agggaaagta
    13981 gaacctggtt gcagcgtagc cattattggt tcaggtccag ttggattggc tgctctttta
    14041 acagcccaat tctattcacc agctaaattg attatggtag acctagacga taaccgtttg
    14101 gaaactgccc tatcattcgg tgcgactcat aaggtaaact cttcagaccc tgaaaaagcg
    14161 ataaaagaaa tttatgattt gacagatggt cgcggtgtag atgtcgctat tgaagctgta
    14221 gggattcctg aaacatttga tttctgtcaa aagattatcg gtgtagacgg aacggttgcc
    14281 aactgtggtg tgcatggtaa accagttgaa ttcgatttag ataaactttg gattcgtaac
    14341 atcaatgtaa caactggttt ggtatctaca aatacgactc cacaattgtt gaaagcactt
    14401 gaaagtcata agattgaacc ggaaaaattg gtaactcact atttcaaact cagtgaaatt
    14461 gaaaaagcct acgaagtctt cagtaaggca gcagaccacc atgccattaa ggtcattatc
    14521 gaaaacgata tctcagaagc ctaagtagta aaaatatttt tgtacataag caaatagaaa
    14581 ttcagtcatc catcagatgg ctggattttt tatcaaaaaa ttaagaaatg agcatatttc
    14641 tttccttgtc tggcggaatt ggttataata tacggtacaa aggaatgaat gaatatgtat
    14701 cgtgttatag aaatgtacgg agattttgaa ccgtggtggt tcttagaagg ttgggaagaa
    14761 gatattgtag caagtagaaa atttgaccag tattatgatg ctctcaaata ctacaaaact
    14821 tgctggttta gattagaaca agaatcccct ctttataaaa gtagaagcga cttgatgacc
    14881 attttttggg acccggaaga ccaacgctgg tgtgatgaat gtgatgagta tttacaacaa
    14941 taccattctt tggctctttt gcaggatgag caggttatcc cagacgaaaa actacgctca
    15001 ggctatgaaa aacaaaccag tcaggaaagg aatcgttctt gccgtatgaa attaaaatag
    15061 agaaaagtaa cttttttgga gttgcttttt ttatttttct aacgctttgc gaatagtata
    15121 ggtgaggagg taagtatggt tcaagaaatt gcacaagaaa tcattcgttc ggctcggaaa
    15181 aaaggggcgc aagacattta ttttgtccct aagttagatg cctatgagct tcatatgagg
    15241 gtaggagacg agcgctgtaa aattggtagc tatgattttg aaaagtttgc agccgttatc
    15301 agtcacttta agtttgtggc gggtatgaat gtgggagaaa aaagacgtag tcaactgggt
    15361 tcctgtgatt atgcctatga ccagaagatg gcgtctctac gtttatctac tgtaggcgat
    15421 tatcgggggc atgagagttt ggttatccgt ttgttgcacg atgaggagca ggacttgcat
    15481 ttttggtttc aggatattga agaattaggc aagcagtaca ggcaacgggg actctatctt
    15541 tttgctggtc cggttgggag tggtaagacg accttgatgc atgaattgtc caagtcactc
    15601 tttaaaggac agcaagttat gtccatcgaa gatcctgtcg aaatcaagca ggacgacatg
    15661 cttcagttgc agttgaacga agcaatcggc ctaacctatg aaaatctaat caaactttcc
    15721 ttgcgtcatc gaccagatct cttgattatc ggagaaattc gtgacagcga gacggcgcgt
    15781 gcagtggtca gagctagttt gacaggtgcg acagtctttt caaccattca cgccaaaagt
    15841 atccgaggtg tttatgagcg tctgctggag ttgggtgtga gtgaagaaga attggcagtt
    15901 gttctgcaag gagtctgcta ccagagatta atcgggggag gaggaatcgt tgactttgca
    15961 agcagagatt atcaagaaca ccaagcagcc aagtggaatg agcaaattga ccagcttctt
    16021 aaagatggac atatcacaag tcttcaggct gagacggaaa aaattagcta cagctaagca
    16081 aaaaaatatc atcaccctat ttaacaatct cttttctagc ggttttcatc tggtggagac
    16141 catctccttt ttagatagga gtgctttgtt ggacaagcag tgtgtgaccc agatgcgtgt
    16201 gggcttgtct caggggaaat cattctcaga aatgatggaa agtttgggat gttcaagtac
    16261 cattgtcact cagttatctc tagctgaagt tcatggcaat cttcacctga gtttgggaaa
    16321 gatagaagaa tatctagaca atctggccaa ggtcaagaaa aagttaattg aagtagcgac
    16381 ctatcctttg attttgctgg gttttcttct cttaattatg ctggggctac ggaattacct
    16441 gctcccacaa ctggatagta gcaatattgc cacccgaatt atcggtaatc tgccacaaat
    16501 ttttctaggc atggtagggc ttgtttccgt gcttgccctt ttagcactaa ctttttataa
    16561 aagaagttct aagatgagtg tcttttctat cttagcacgc cttcccttta ttggaatctt
    16621 tgtgcagacc tacttgacag cctattatgc acgtgaatgg gggaatatga tttcacaggg
    16681 aatggagctg acgcagattt ttcaaatgat gcaggaacaa ggttcccagc tctttaaaga
    16741 aatcggtcaa gatctggctc aaaccctgaa aaatggccgt gaattttctc agacgatagg
    16801 aacctatcct ttctttagga aggaattgag tctcatcata gagtatgggg aagttaagtc
    16861 caagctgggt agtgagttgg aaatctatgc tgaaaaaact tgggaagcct tttttacccg
    16921 agtcaaccgc accatgaatt tggtgcagcc actggttttt atctttgtgg cactgattat
    16981 cgttttactt tatgcggcaa tgctcatgcc catgtatcaa aatatggagg taaattttta
    17041 aaatgaaaaa aatgatgaca ttcttgaaaa aagctaaggt taaagctttt acattggtgg
    17101 agatgttggt ggtcttgctg attatcagcg tgcttttctt gctctttgta cctaatctga
    17161 ccaagcaaaa agaagcagtc aatgacaaag gaaaagcagc tgttgttaag gtggtggaaa
    17221 gccaggcaga actttatagc ttggaaaaga atgaagatgc tagcctaagc aagttaaaag
    17281 acgatgggcg aatcacggaa gaacaggtta aagcttataa agaatacaat gataaaaatg
    17341 gaggagcaaa tcgtaaagtc aatgattaag gcctttacca tgctggaaag tctcttggtt
    17401 ttgggtcttg tgagtatcct tgccttgggc ttgtccggct ctgttcagtc cacttttgcg
    17461 gcagtagagg aacagatttt ctttatggaa tttgaagaac tctatcggga aacccaaaaa
    17521 cgcagtgtag ccagtcagca aaagactagt ctgaacttag atgggcagat gattagcaat
    17581 ggcagtcaaa agttgccagt ccctaaagga attcaggccc catcaggcca aagtattata
    17641 tttgaccgtg ctgggggcaa ttcgtccctg gctaaggttg aatttcagac cagtaaagga
    17701 gcgattcgct atcaattata tctaggaaat ggaaaaatta aacgcattaa ggaaacaaaa
    17761 aattagggca gtgattttac tggaagcagt agtcgctcta gctatctttg ccagcattgc
    17821 gaccctcctt ttgggacaaa ttcaaaaaaa taggcaagag gaagcaaaaa tcttgcaaaa
    17881 ggaagaagtc ttgagggtag ctaagatggc cttgcagacg gggcaaaatc agataaacat
    17941 caacggagtt gagattcagg tattttctag tgaaaaagga ttggaggtct accatggttc
    18001 agaacagttg ttggcaatca aagagccata aggtcaaggc ttttaccttg ttagaatccc
    18061 tgcttgccct gcttgtcatc agtgggggct tactcctttt tcaagctatg agtcagctcc
    18121 tcatttcaga agttcgctac cagcaacaaa gcgagcaaaa ggagtggctc ttgtttgtgg
    18181 accaacttga ggcagaatta gaccgttcgc agttcgaaaa agtagaaggc aatcgcctat
    18241 acatgaagca agatggcaag gacatcgcca tcggtaagtc aaagtcagac gattttcgta
    18301 aaaccgatgc tagtggacga ggttatcagc ctatggttta tgggctcaaa tccgcacaga
    18361 ttacagagga caatcaactg gtgcgctttc gtttccagtt tcaaaaaggc ttagaaaggg
    18421 agttcatcta tcgtgtggaa aaagaaaaaa gttaaggcag gtgttctcct ctacgcagtc
    18481 accatagcag ccatctttag tcttttgttg caattttatt tgaaccgaca aatcgcccac
    18541 tatcaagact atgctttaaa taaagaaaaa ttggttgctt ttgccatggc taaacgaacc
    18601 aaggataaag ccgagcaaga aagtggggaa caggtcttta atctaggtca ggtaagctat
    18661 caaaacaaga aaacaagctt ggtgacgact gttcgtacgt ctaagagcca atatgagttt
    18721 ctgtttcctt cagtcaaaat caaagaagag aaaagagata aaaaggaaga ggtagcgacc
    18781 gattcaagcg aaaaagtgga gaagaaaaaa tcagaagaga agcctgaaaa gaaagagaat
    18841 tcctagtcaa ttcaactata atgcgttgaa tccagaatag ttcactgtag tttctagaaa
    18901 attgctggaa atggatgtta agctccaatt tatttgttta tatcttattt cagtttacta
    18961 tactttgtgc taaactaaag atatgaaaca tgattttaac cacaaagcag aaactttcga
    19021 ttcccctaaa aatatcttcc tcgcaaactt ggtatgtcaa gcagtcgaga aacagattga
    19081 tattttatca gacaaagaaa ttttagattt cggtggtggc acgggtctat tagccttgcc
    19141 cctagccaag caggctaagt cagttactct tgtagacatt tctgagaaaa tgttggagca
    19201 agctcatttg aaagcggagc agcaagcaat caagaatatc cagtttttgg agcaagattt
    19261 accgaaaaat cccttggaga aagagtttga ttgccttgct gttagtcggg ttcttcatca
    19321 tatgcctgat ttggatgcgg ctctctcact gtttcatcaa catttgaagg aagatgggaa
    19381 actcatcatt gctgatttta ccaagacaga agctaatcat catggatttg atttagctga
    19441 actggaaaac aagctaattg agcatggttt ttcatctgtg catagtcaga ttctctatag
    19501 cgctgaagac ttgtttcaag gaaatcactc agaattcttt ttaatagtag cccaaaaatc
    19561 actcgcctag tcagggagtg atttttctat aaggatggaa aaaagaaggg aaatttgata
    19621 agataggaat atggattttg aaaaaattga acaagcttat acctatttac tagagaatgt
    19681 ccaagtcatc caaagtgatt tggcgaccaa cttttatgac gccttggtgg agcaaaacag
    19741 catctatctg gatggtgaaa ctgagctaaa ccaggtcaaa gacaacaatc aggcccttaa
    19801 gcgtttagca ctacgcaaag aagaatggct caagacctac cagtttctct tgatgaaggc
    19861 tgggcaaaca gaacccttgc aggccaatca ccagtttaca ccggatgcta ttgctttgct
    19921 tttggtgttt attgtggaag agttgtttaa agaggaggaa attactatcc tcgaaatggg
    19981 ttctgggatg ggaattctag gcgctacttt cttgacctcg cttgataaaa aggtggatta
    20041 cttgggaatg gaagtggatg atttactgat tgatctggca gctagcatgg cagatgtaat
    20101 tggtttgcag gctggctttg tccaaggaga tgccgttcgt ccacaaatgc tcaaagaaag
    20161 cgatgtggtc atcagtgact tgcctgtcgg ctattatcct gatgatgccg ttgcgtcgcg
    20221 tcatcaagtt gcttctagcc aagaacatac ttacgcccat cacttgctca tggaacaagg
    20281 atttaagtac cttaagtcag atggatatgc tatttttcta gctccgagtg atttgttgac
    20341 cagtcctcaa agtgatttgt tgaaagtctg gttgaaagag gaggcaagtc tggttgctat
    20401 gattagtctg cctgaaaatc tctttgctaa tgctaaacaa tctaagacta tttttatcct
    20461 acagaagaaa agtgaaatag cagtggagct ttttgtttat ccacttgcta gcttgcaaga
    20521 tgcaagtgtt ttaatgaaat ttaaagaaaa ttttcaaaaa tggactcaag gtactgaaat
    20581 ataaaataga ttttgttata atagttgaaa acgcttaaaa aggggtatca tgttatgaca
    20641 aaaacaattg caatcaatgc aggaagttca agcttaaaat ggcaattata cttaatgcca
    20701 gaggaaaaag tattggcgaa aggcttgatt gaacgtatcg gtttgaaaga ttcaatttca
    20761 actgtaaaat ttgacggccg ttctgaacaa caaattttgg atatcgaaaa tcatacacaa
    20821 gccgttaaaa ttttattgga tgacttgatt cgtttcgata ttatcaaggc ttatgacgag
    20881 attacaggtg ttggccaccg tgttgtagca ggtggagaat atttcaaaga atcaacagtt
    20941 gttgagggag atgttttaga aaaagttgaa gagttgagtt tgttggctcc tctacacaac
    21001 ccggccaatg cagcaggtgt tcgtgccttc aaggaattgt tgccagacat taccagtgta
    21061 gttgtttttg atacttcctt ccacacaagt atgccagaga aagcttatcg ctaccctcta
    21121 ccaacaaaat attacacaga aaacaaggtt cgtaaatacg gtgctcatgg tacaagtcac
    21181 cagtttgtag caggagaagc tgcaaaactc ttgggacgtc cattagaaga cttgaagtta
    21241 attacctgtc atattggtaa cggaggctca attacagctg tgaaagccgg caaatctgta
    21301 gacacttcta tggggttcac tcctcttggt ggtattatga tgggaacgcg tacaggggat
    21361 attgatccag ctatcattcc ttatttaatg caatatacag aggattttaa cacaccagaa
    21421 gatatcagtc gtgttcttaa ccgtgaatca ggtcttttgg gagtttctgc taattctagc
    21481 gatatgcgcg atatagaagc agctgtagca gaagggaatc acgaggctag cttggcttat
    21541 gaaatgtatg ttgaccgtat ccaaaaacat atcggtcagt accttgcagt gctaaatgga
    21601 acagatgcca ttgttttcac agcaggtgtc ggtgaaaatg cagagaattt ccgtcgtgat
    21661 gtaatctcag ggatttcgtg gtttggttgt gatgttgatg atgaaaagaa tgtctttggc
    21721 gttacaggag acatctcaac agaggcagct aaaatccgtg tcttggttat tccaacagat
    21781 gaagaattag tcattgcccg tgacgttgaa cgcttgaaaa aataagtgaa actaaaaaaa
    21841 tattcaatac aaggagttgg gaaagttatt tttccagctt ctttttctga tgaaattgtc
    21901 caaaaccttg ctatgattgg ctttt
//
LOCUS       DAVLYR010000015        21848 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLYR010000015 DAVLYR010000000
VERSION     DAVLYR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.015"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(131..2146)
                     /gene="recG"
                     /locus_tag="VYO63_000528"
     CDS             complement(131..2146)
                     /gene="recG"
                     /locus_tag="VYO63_000528"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1671503.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHYMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIETWQEDPEWRMIALHLEKKEHLD"
     gene            complement(2165..3268)
                     /locus_tag="VYO63_000529"
     CDS             complement(2165..3268)
                     /locus_tag="VYO63_000529"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1671504.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(3258..3626)
                     /locus_tag="VYO63_000530"
     CDS             complement(3258..3626)
                     /locus_tag="VYO63_000530"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1671505.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(3665..4696)
                     /locus_tag="VYO63_000531"
     CDS             complement(3665..4696)
                     /locus_tag="VYO63_000531"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1671506.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(4698..5729)
                     /locus_tag="VYO63_000532"
     CDS             complement(4698..5729)
                     /locus_tag="VYO63_000532"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1671507.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(5810..8323)
                     /gene="secA"
                     /locus_tag="VYO63_000533"
     CDS             complement(5810..8323)
                     /gene="secA"
                     /locus_tag="VYO63_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1671508.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(8469..8756)
                     /locus_tag="VYO63_000534"
     CDS             complement(8469..8756)
                     /locus_tag="VYO63_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671509.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(8767..9417)
                     /locus_tag="VYO63_000535"
     CDS             complement(8767..9417)
                     /locus_tag="VYO63_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1671510.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(9421..10062)
                     /locus_tag="VYO63_000536"
     CDS             complement(9421..10062)
                     /locus_tag="VYO63_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671511.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(10055..10846)
                     /locus_tag="VYO63_000537"
     CDS             complement(10055..10846)
                     /locus_tag="VYO63_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671512.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILHVVPYMVLKKYEDNV"
     gene            complement(11031..11366)
                     /locus_tag="VYO63_000538"
     CDS             complement(11031..11366)
                     /locus_tag="VYO63_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671513.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(11506..11841)
                     /locus_tag="VYO63_000539"
     CDS             complement(11506..11841)
                     /locus_tag="VYO63_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671514.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(11932..13242)
                     /gene="der"
                     /locus_tag="VYO63_000540"
     CDS             complement(11932..13242)
                     /gene="der"
                     /locus_tag="VYO63_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1671515.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(13256..13969)
                     /locus_tag="VYO63_000541"
     CDS             complement(13256..13969)
                     /locus_tag="VYO63_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1671516.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAKMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(13966..14862)
                     /gene="dnaI"
                     /locus_tag="VYO63_000542"
     CDS             complement(13966..14862)
                     /gene="dnaI"
                     /locus_tag="VYO63_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1671517.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(14863..16032)
                     /locus_tag="VYO63_000543"
     CDS             complement(14863..16032)
                     /locus_tag="VYO63_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1671518.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(16033..16506)
                     /gene="nrdR"
                     /locus_tag="VYO63_000544"
     CDS             complement(16033..16506)
                     /gene="nrdR"
                     /locus_tag="VYO63_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1671519.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            16646..17011
                     /locus_tag="VYO63_000545"
     CDS             16646..17011
                     /locus_tag="VYO63_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1671520.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            17016..17711
                     /locus_tag="VYO63_000546"
     CDS             17016..17711
                     /locus_tag="VYO63_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671521.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            17728..18495
                     /locus_tag="VYO63_000547"
     CDS             17728..18495
                     /locus_tag="VYO63_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671522.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(18848..20047)
                     /locus_tag="VYO63_000548"
     CDS             complement(18848..20047)
                     /locus_tag="VYO63_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671523.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(20044..20937)
                     /locus_tag="VYO63_000549"
     CDS             complement(20044..20937)
                     /locus_tag="VYO63_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671524.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            21053..21133
                     /locus_tag="VYO63_000550"
                     /pseudo
     CDS             21053..21133
                     /locus_tag="VYO63_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            21643..21831
                     /locus_tag="VYO63_000551"
                     /pseudo
     CDS             21643..21831
                     /locus_tag="VYO63_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         6438 a         4895 c         4043 g         6472 t
ORIGIN      
        1 ccttcttttg gaaagtggtt tggtaattta ctttaatcgt tactgtcata tgagagtcct
       61 cgtttctttt tgatgacttt agtataagag ccaaatctaa aaactagctt aagttttcct
      121 tagagaaagc ttaatccaga tgttctttct tttccagatg aagggcaatc atgcgccact
      181 ctggatcttc ttgccaagtt tctatagaac taatgtagct agcaaccttt cttgcttctt
      241 ctaaaatcgg aaaatcttcg ataatatcag ccacttggaa ctctggaagt cctgactgtc
      301 tggttccaaa aatctcacca gaaccacgca ttttcaaatc ttcctccgca aggacaaatc
      361 cattggtcgt ttctgtcatg atgcgcatgc ggtctttccc agaatccgtc ttgggattgg
      421 caacgagaac agcgtaggac tgcttgtccc cccgaccgac acgacctcta agctggtgaa
      481 gttgactgag accgaagcga tcggcatcca tgataatcat gacagtcgca ttgggaacat
      541 tgaccccaac ctcaataacc gtcgtcgaaa ccagaatatc cgtctttctc tccttgaaat
      601 cctgcatgat ctggtctttt tcgtcactct tcatcctacc atgtagaaga gccacctctg
      661 ccttgcctgc aaaatgagtc gtcaactcct ctgataaggc aatggcattt ttcaaatcta
      721 gagcttctga ttcttcaatc aaaggagaga tgacataggc ttgggaacct ttttgaattt
      781 ccccctctaa ccaagtcaag acctgaggta gttgctcatg tttgatccag cgcgtcacaa
      841 taggcttccg acctgctggc atctggtcga taatggaaac atccatatct ccaaaggctg
      901 tgatggcaag cgtccgtgga atgggagtcg ccgtcatcat gaggacatct ggattgtcgc
      961 ctttttcccg taaaatacgc ctttgcccca caccaaaacg gtgctgctca tcgataataa
     1021 tcaaaccaag acgagcatac tccaccccat cttgtatcag agcgtgagtt cctataatca
     1081 aatcagcctc acccttggca atggtctcca agacctctct cttttctgca gctttcaagg
     1141 aacctgtcaa gagagccagt ttcaaattgg gaaaaaggtt ctgtaaactc tcaaagtgtt
     1201 gctctgcgag gatttctgtt ggtaccatta gggcagcctg ataacctgct gtcactgccg
     1261 caaacatggc caagccagcg actaccgttt ttccgctccc cacatctcct tgtaggagac
     1321 gattcatgta gtggtcggac ttcatatcag ttaaaatttc ctgcaaactc ttttcctgag
     1381 cttgggtcag ggcaaaagga agacttgctt taactgctgt cactttttcc tgagaccaat
     1441 tcagaaccag accacttccc tgaactctat tttcagactt gagcatctgc aactgcattt
     1501 ggaaataaaa gagttcctca aacttgatac ggcgaagagc ctgcttgtat tctgccaaat
     1561 cctttggaaa atgcatagct cggactgcct gacaacggga catgagtttg tatttgtcta
     1621 gtaaagactg gggcagattt tcttctatca agaggtccag tccctgatca aaagccgtct
     1681 tgatgacctt gaccagactg gcctgactga ttccctgagc cagacgatag acaggctgga
     1741 ggtcatcttc tacctgagcc agaaccttca tcccagtcag actagcctta gcgcggtccc
     1801 attttccaaa gacagcaagg gttgctccca actctatttt atcagccaga tagggctggt
     1861 taaagaaatt caccgcaaaa acgacctctc cctgcttgag actaaaacgc aggcgattgc
     1921 gcttgaaacc ataatactgg acactagcag gagtcactac ctgaccagaa agaactgcct
     1981 tctcaccgtc ttctagttcc agcacctgct tggttttgaa gtcttcataa cggaaaggaa
     2041 agtagagcaa gagatcttgc aagttttcaa ttcctagttt ggcgtatttt tctgctgact
     2101 ttggtcccac accaggcaag acatgcaagg gttgatgtag attcatgctc cactcctttc
     2161 ttttctaata atattctctc ggaatacggt cgctgaggag gcaaaccacc tcatagttaa
     2221 tggttacgcg gtaggtcgct acctgagttg cagtgatttc cttatcccca ttggagccaa
     2281 tcaaggttac cttggttccg agtgggtaag gcttaggcaa tcgaatagtg atttggtcca
     2341 tcgaaaccct gccgacaatt gggcaagctt ggccatctac caagacagag aaattttgca
     2401 tgtctcttgt ccatccatct gcatacccga ttggcacggt cgcgatgact tgctcgctat
     2461 ccgtttgata agttgctcca tagcccatgc aagcaccagc tggaactgtc ttgatatgaa
     2521 ccagagcaga ctccaaggtc aaggccggta tcaaatcata aggcaaagcc aagaccgctc
     2581 cacttggatt gagaccatac atggcatctc ccatacgaac cgcattgaaa atagtctcta
     2641 catgccaaag agtcgttgca gaattgctag catgaaccag ctctggaact tccttcatac
     2701 tagctaaaat agttttaaac cgttctaact gggcattaaa atagtcatct gattcctcat
     2761 cagcagtagc aaagtgggta aagattcctt caacacgaac accgtgttgt tggagcaaat
     2821 cttgagcctg ctcaacctca cttgcctctc taaaaccaat ccgtcccatc cctgaatcaa
     2881 tcttgaggtg gactgtcaat ccagttaggt ccacttcctt atctaagagt gcttgaatcc
     2941 actccagtcc agccactgtc aaggtgaagt catattcttt agctagagca acagcttcga
     3001 tttcagaaac tcctaaaatg aggattggct tgctgagtcc agcttgtctg agttcaatgg
     3061 cttcatcgat attggaaacg caaaagccat caacatcatc ttgaattgcc ttggcaacgg
     3121 caacagctcc atgaccataa gcattggcct taaccacagc caacttgagc gttccttgag
     3181 ggatatgagc ccccatttgc tgaatatttt gtcgaatagc tcccagatga atcagagcct
     3241 tggttggtct atgtggacta gctttcatga ttttcctcca aaatgacact ggctgtcaca
     3301 aactgatcgg tgtggctgat agacagccaa atctttcctg aaaatggtgc ctgactaaaa
     3361 taaggcgccc cacgttcatt gttcaatact tccaaatcct gaaaaccgag cttgctaatg
     3421 cccgttccca tagccttgga aaaggcctcc ttagccgacc agcgaccagc taaatattct
     3481 atttgcctgc gtcctttgag actggtgaag cgctccattt cctgagcggt cagtacacgc
     3541 ttagcaaatc cttcatgtcg tgtaactgcg ctttctatcg aagccaattc ttcgatgtca
     3601 attccgtgtc caactatcat tctcatcaaa aggagttgag attccccaac tccatccttt
     3661 tcacttattt tgtcaaggta acataaatct cttctaccaa ggcctctgta ttttcccaac
     3721 ctaggcaagg gtcagtaata gagcaaccaa agatctctgg ttggttttga cgaccatctg
     3781 ctaggtaaga ttcaatcata aatcctcgaa ccgtcttttt aattttctca ttccaatcac
     3841 gattctgcaa ggtctggcga acaattcgaa tctgctccat atattgcttg cctgagttat
     3901 catggttggt gtcaatgagg ataaaaggat tttcaagtcc catggtttca tagcgttcaa
     3961 tggcttggag taggttttca tagtagtaat tcggcatata attgccatac tcgttgactg
     4021 ctccacggag gataacatgg gccaaaggat tacctgatgt ctcaacttcc tgcccatgat
     4081 aaaggaaggt ttgcttgttt tgagcagcat agatggcgtt aaacataaca cccaaatttc
     4141 ctgaggttgg atttttcatc cctactggtg catcaatccc agaagccaca aagcggtgct
     4201 cttggtcttc cacagaacga gctccaacgg catggtagct gaccaagtca tccaccaaga
     4261 tcagatttga cggataaagc atctcatctg ccgttgtcaa accagtctct gtaatcacgc
     4321 ggtaatgcaa ctggcgcaca gcctgcaaac cgttaatcag gcttggagcc ttagaagtat
     4381 ctggctggtg aaccaatcct ttatagccgt ctccattggt acgaggctta gcagtataca
     4441 cgcgcatgac catgaaaatc ttgtccgcta ccttcttttg caaggcggat aaacggcgag
     4501 catattccaa gaccgcctct tcattatcag aagagcaagg accaatcacc aagagaatac
     4561 ggtcatcttc ccctgaaata atatctgcca attccctgtc acggctttcc ttgagtctca
     4621 aggcttccgc agacaattgg gtttcagcct tgatgacttc catatcgatt tcttgacctt
     4681 tttcaataaa tgccatctta ttctcctaac gttttgtaaa tttctctgac aagagcttct
     4741 gtgttatccc aacccaggca agggtctgtg atagacttgc caaatacttc tggctcattt
     4801 tgtcgaccat cttccagata agactcaatc ataaaaccac gaacgaactg cttaattttt
     4861 tcattccaag cacggttaat caaggtctgg cggacaattc ggatctgttc aatatactgc
     4921 ttaccagaat tgtcatgatt ggtatcaatg atgataaaag gattttccaa gcccattttc
     4981 tcatattggg caatggtatc aattaaattg tcataatagt agttgggaat attttttcca
     5041 tactcattaa gagcaccacg aagaatagcg tgtgaaagcg ggttcccagt tgtttctact
     5101 tcttttccta agaaaaggaa actttgtttg ttttgagcag cataaatccc attaaacatg
     5161 acattgagat ttccagaggt tggattttta aaaccagtag aaaatcctgc cccacttgcc
     5221 acaaagcggt gttgctggtc ttcaactgaa cgggcaccaa ctgccatgta agaaatcaaa
     5281 tcatctacaa gcggaaggtt ttcaggataa agcatttcat cagcagttgt catccctgtt
     5341 tctgtgatga cacgatagtg aagatggcga acggctttga ttccattgat aagactaggc
     5401 gcttctgtcg cgttaggctg gtgaatcaag cccttatagc catctccgtt ggtacggggt
     5461 ttggcagtat aaacacgcat aaccataaag atacgatctg ccacttcttc ttgtaggact
     5521 gccaaacgct tagcgtattc aaggacagct tcttcgttgt cagatgagca tggcccgatt
     5581 accaagagaa ttcgctgatc ttctccacgt ataatggctt ctagctcttg atcgcgctgt
     5641 gatttcctct ccaaagcctg accttctaat tttgacaagg cacgaacttc ttctatattt
     5701 attttagagc tttttgctgt aaataccata actcatctcc ttataaagca aacggatacc
     5761 cagtaaaaat ttacttttca caagatatcc gcctctaaac tatctcattt tattgtcttt
     5821 taccgtgaca gtttttaaat ttcttaccag aaccacatgg gcaaagttca ttgcgtccaa
     5881 tctggctcaa atccaaatct tctggcatac ttgcttggtg agcagcgata ttgcgagtcg
     5941 ctgttgtact gatatgacgt tctgcctgtg gtctttcttg ttcatgaatt tgtgctttca
     6001 tcatcaagcg tgtcacatca aactcaatcg aaccaatcat atcattaaac atacggaaac
     6061 cttctgcctg atactcaaca acagggttgt tctgagcata gccacgaagt ccaaccgcgt
     6121 tacgcaattg atcaagggca tcgatatgat ctgtccactt gttatccacc actcgtagaa
     6181 tcaaaacttt ttggaattct ttaactgctt cttcatcgcg tagttttgaa acctgactat
     6241 cgtaaacctt caaggcacgt tggaaaagct cttccttgat ggccttatca gacaagcctg
     6301 acaagtcttc catcgtaata gaatcttcag gaagcaagtt gtacttagca aagttcaaaa
     6361 ttgcctctag tttttcatct tgtttggcac gcgcatgacc atcaacgaca cgttcaatcg
     6421 tgcgtttgat catagcctga atttcaggtg ccaagtcacg atctgcagtg atgacatcgt
     6481 aacgttgagc atagataatc tcacgttgtt cacgcatgac atcatcgtat tgaaggactt
     6541 gtttacgggt atcgtagtta tttccttcga cacgtttctg agctgcttca acctgacgcg
     6601 tcaacatgcg agactcaatc gcctcttcag acatgttcaa acgttcaaag attcccttca
     6661 agcgttcaga accaaaacgt ttcatcaaat catcttcaag agataggtag aattgtgact
     6721 cacctggatc tccttgacga cctgaacgtc cacgaagctg gttatcgata cgacgacttt
     6781 catgacgttc tgtaccaata acacaaagtc ctccaagttc acgaacacct tcaccaagct
     6841 tgatgtcggt accacgaccc gccatgttgg ttgcgatggt aacggcacca cgttgaccag
     6901 cattcatgat gatttgggct tctctatagt ggtttttggc attcaagact tcgtgaggaa
     6961 caccagctgc aaccaatttc ttagaaatgt agtcactagt ttcaaccgcc actgtaccaa
     7021 ccaagacagg ttgacccttt tggtaacgag ccttaacgtc ttcgacaacc gctttaaact
     7081 tagattcgat acttgcataa agaaggtctg agtggtcaat acgttgaaca ggacggtttg
     7141 ttgggattgg aataacacga atgttgtaga tttcacggaa ttcttcttcc tcagtcttac
     7201 ctgtacccgt cataccagac aatttcttgt acatacggaa aaggttttgg tacgtgattg
     7261 aggcagatgt cttggtttca tcctggattg gcacaccttc tttggcttca atagcttggt
     7321 gcaatccatc agaataacga cgaccttcca tggtacgacc tgtaaattgg tcgacaatca
     7381 agatttcttg ctcttcgctc accacatagt caatatcgag aagcatgatg tagttggcac
     7441 gaagggcgtt atcgataaag tgagtcaaag ccacgttttc gatgtcatag aggttttcaa
     7501 gtttgaagta gctttcagcc ctgtcaatcc ctgaatcaga caaaccaata gtcttagact
     7561 gcacatcgat gatgtagtca tctttgttca aagattttac atagtggtct gccatgtgat
     7621 acaactgact ggtttcaacc gcattggcac ctgatacaat caaaggtgta cgagcctcgt
     7681 caatcaagat agagtcaacc tcatcgacca aggcatagtt aagcggacgt tgtaccatgt
     7741 tttcggcgcg aacgaccatg ttgtcacgaa ggtagtcaaa tccgatttct gagttagttg
     7801 agtaagtaat atcacactca taggcttctt ttttctccat tggagatttg gtagccaagt
     7861 taatccctac tgacaaacca agccaagagt acaattcacc catctcagtc gcgtcacgtt
     7921 ctgacaggta ttcattaacc gtaactacgt gaaccccttt acctgaaagg gcattgaggt
     7981 ataccggcat ggtcgcagtc aaggttttcc cttcccctgt acgcatctct ggcacgtcac
     8041 catggtgaag aacaatcccc cccatgacct gaaccttata agggaagaga cctaggacac
     8101 gtttggcacc ttcacggaca accgcaaatg cttcgtaaag caatgaatcc agtgattctc
     8161 cattttgata acgttcctta aattcaactg tttttgcttt tagttggtcg tcagtcaaag
     8221 tagccatttg gtcttcgtat ttgaaaacct tgtcagccat cttttccaga cgacggattt
     8281 ctcctttatc attttcgata attgttttta aaatattagc catgtttttc cttactttaa
     8341 attccgaata ttttagaatg ttcctttaat tttagcacaa ttactgatta ttttcaagga
     8401 agaatcccca ttttgaaaag gaaaaagagg ttagttttca agctaacctc tccgactttt
     8461 atgactgttt aattgccaaa aatcggcaaa atggcaaatg tgataaataa gttaatccaa
     8521 agagaaagac agcaaatcag tccaaaaaca aagagactga ctttcttgga cagcaaggcc
     8581 atgagaatgg acagaattcc aaaagctagc aagattttct gcatgatgaa gatgtcaatc
     8641 cttaccccca gaatcgctct atcccaaggg ataaagaaga aagacagcca gatcagcaga
     8701 actaaaccaa tataaacctt ggaatagcgt gtaataaatg attttagatg tgccataagc
     8761 catctcctag gattgtatat cctttacata ttgtatatgt ccattttcga caactaatac
     8821 atctgtacaa aggtctgaaa tatcttccga tatgtgcgac gtcaggataa ccagaccatt
     8881 ttcttttttc aggtaagata aaatgacctg tttggttaac ctcacactct tctcatccaa
     8941 agcattcata ggttcatcga gaaagagtat agaaggagag gaaataaagg cttgaatcaa
     9001 ggccattttt tgctttgttc ccaaggataa atgacggtat tcaacgtctt caaattcctg
     9061 taaatcatag tattgaatcc aataggcaat tcttttttcc gtaactttag atgaaaaata
     9121 ccttaacagt tccaaatttt ctctcaggga taaatgagat aaaaactcga ctttttcaat
     9181 taaaattcct gcatcctgaa tataatgatt ttttaccccg taaactttac catcttgaag
     9241 aacttttcct ttgtcaagct taatataacc agcaagtatc tttaaaagaa ccgtcttgcc
     9301 agatccatta tcacctttaa gtccataaat ttttcctgat tgaaacacca aattcaactg
     9361 atttaaaata ggtcttttcc cataaatttt ctgtccattt ataatttcaa gtctcatcgt
     9421 ttatcctttc ttaaatttct cttccaagcg actaacaatc aacataacta ataagcttaa
     9481 gagtactaaa tacactgtta caatatgatc catcatccat tcttgtccca caaatgccaa
     9541 aaaatagaga aaaaaagaaa aaccagcgcc cgcactaatc agtgctatca gataagacag
     9601 tagtaagtac aacatcatta actgagcaag taaaaagaaa agtgcgactg ttttgaaatg
     9661 aattcctagc ggaagtaaac caagaaacca gacaaacaaa tcttgagtaa aaaatttaga
     9721 aatgacaacc aatcgtttcc ttgtataatc gaaaaaggag cttgattgat agcgaatcaa
     9781 gtgatggtaa tggtctgcca aatcactttc aagtaaaatg acagagagcc atcctaccat
     9841 gaaaagcgaa aggtttaaca agagtttggg gatttctaaa ttcgcttcag aatagtcatt
     9901 ataggaaaaa attgaaatca attctctctt gaaaaagata acagcatcct gcttttgaac
     9961 cgctgttgac aatagatagt ttatcaaaat catcatcaga actatacgaa gtagtaaaac
    10021 gatctttctc tttagccaaa tattgttaaa attcttaaac attatcttca tattttttaa
    10081 gtaccatata agggacaaca tgaaggatat aaggagtaaa gagtttcatc aaacttacat
    10141 ccccatagct agcttgcatc aaactcgtca taggaaccat atagtaaggc aaggcactat
    10201 agagcagcat agaaccaagc caaagaaaca tcaaataggt gattgccgaa cgagtcacat
    10261 tccccacata atcaactatt tgtaaaaaat agattgcatt gatgaaaata ccgattagta
    10321 ggacacaagt aaagaacaaa tagcttttta tctctccatc taggagtctt tgtaaaccac
    10381 tcccatcaga aaatagagaa ttccattcaa gaggagaaaa agtcccaaag aaataggtta
    10441 taattgcaat tatcagcaca gtcactaaat atatcaaaca agggatactt gcaacttgca
    10501 aagtcaactt tctttttaac ccttgatagc tcacttctct tccaatactc aatcgtaaaa
    10561 ctttattttt cagctcaata tattgaaaac ctagcaaaac aatgatgatt ggaataataa
    10621 atgattgata aaaacctaga ttgaactgga gaatacgaaa atctaatgct gattttaata
    10681 agtcattttt aattgcttgt ccatcgaatt tagctagatc gggcgcatga atatcaaaaa
    10741 agaaataagt catcaaaaca tcacgataaa ccaaagcaag aaaaagcaaa gtaagcaaga
    10801 cataaacaaa tttatgcctt gccaaagtta agagaggaat cttcataagc cacctcctac
    10861 aaatagaaag taatataata ctcaatgaaa atcaaagagc aaactaggaa actagccgca
    10921 agttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag ttacatatat
    10981 aatccaaggc gacgttgacg tggtttgaag agattttcga agagtataaa tcaatgcctt
    11041 ccgcctttag acttccctag ttcccgtctc aagtcaagca tttttttgaa acagatatag
    11101 tctaaccagt cactagcaaa agcgataagt agtaataata gccaaatacc ccataacttg
    11161 atatctgtca aatttctcaa gacgatattg aaaaacagaa ctgaaaaaac tgtccaagca
    11221 aggctaaaaa gagcatagag ggtgatgtaa atccagtaaa aatagtaaaa aattgggaaa
    11281 atcttactat ctctgtttgc cttttcaatc cagctgaaaa agtaaaaaca gggtaataag
    11341 attatcattt taaacaagta ttccatcaca aacctcctct tttaaattgt tttctttagt
    11401 ttcgttctat taaatgttat taccaatata aaccttggaa tagcgtgtaa taaatgattt
    11461 tagaggtacc ataagccacc tcctacaaat agaaaccgat ataaatcaat gccttccacc
    11521 cttagacttc cctagttcct gtctcaagcg aaacatttct ttgaaacagg aataagttaa
    11581 ccaattcata ccaatagcta gcagaataaa aagaaaccaa atgccccata acttgatatc
    11641 tgtcacattt ctcaagacgg tattgaaaaa cagaactgaa acaactgtcc aagcaaggct
    11701 aaaaagagaa tagaagggga tgtaaaacca gtaaaaataa taaaaaattg gaaaaaactt
    11761 actatttctg ttggccttct caatccagtt atcaaaataa aagtacggtg ctaaaagtaa
    11821 gaatttaaac aaatgttcca tcaccgacat caccctcctt cttttgatag cgttttctat
    11881 tattttatta tatcaaaaaa atccggaact gtcattccag attctacttt tttatttgcg
    11941 ttttcttgcg atgagatgaa tcggtgttcc ctcaaaaaca aaggccttgc ggatttgatt
    12001 ttccaagaaa cgcaggtaag aaaagtgcat gagttcttct tcattgacaa agatgacaaa
    12061 ggttggtggt ttggttgcca cttgggtcgc atagaaaatc ttgagacgtt ttcctttgtc
    12121 tgtcggtgtt gggttgatgg caatggcatc catgatgaca tcgttcaaga cagctgatgg
    12181 aatacgtgta ttttgacttt cgctgatttg cttaatcatc tcaggaagtt tgtggagacg
    12241 ttgcttggtt aaagctgata caaagataat cggtgcgtaa ggcaggtatt ggaactgctc
    12301 acggatatct tcttcccagt ttttcatagt gtggttatct ttttcaagcg tatcccactt
    12361 gttgaccacg ataatcatcc ctttaccagc ttcatgggca aatcctgcga tacgcttgtc
    12421 gtactcacga atgccttctt ccgcattgat gaccatcaag accacatctg aacggtcaat
    12481 agcacgcatg gcacgcataa cagagtattt ctcagtattt tcataaacct taccagactt
    12541 acgcatacca gccgtatcaa tcatggtaaa ctcttgacca tctgtatctg taaagtgggt
    12601 atcaatggca tcacgagttg ttccagcaac aggactagca ataacacggt cttctcccaa
    12661 gatagcattg atcaagcttg attttccaac gttaggacga ccaatcaagc taaacttaat
    12721 gacatctgga ttttcttcct catattcatt tggaagattt tctacgatcg catctagcac
    12781 atcccctgta ccgattccat ggacagatga gataggcaat ggttcaccca aaccgagagc
    12841 atagaaatca tagatatcat ttctcatctc agggttgtcc accttgttga ctgcgaggat
    12901 aactggtttg tgggtcttat aaagcttacg ggctacgtat tcgtctgcat cggtaattcc
    12961 ttccttaccg gacacgacaa agacgataac atctgcttct tccatggcaa tttctgcctg
    13021 gtgcttgatt tgttccatga aaggagcatc gacatcatca attcctcctg tatcaatcat
    13081 gctaaaagaa cgattgagcc actcacccgt tgcataaata cggtcacgtg tcactccttc
    13141 gacatcttct acaatggaga ttcgctcacc agcgatccga ttaaataggg ttgatttccc
    13201 aacattggga cgtcctacaa tggcaatagt tggtagggcc ataatttctc actttctaca
    13261 ataatttctt ctgttcaaga ttttttctag ttgagcttgg ttcagcttga ccaaactgtt
    13321 ctgctaggcg ctgactccag cttgtggtcg cacgcgcccc agcatagtca gcctgaacac
    13381 ggtcataagc ttggattgcc tcagttgact gttcttggta ttcttcctca aagacaacat
    13441 tctctagtgg cagtctcggt ttcatatcat gatgttgatt tggcacaccc agtgccatcc
    13501 caaagacaga ataggtgtag tcaggtaggt taaagagctc tgccacttct tcagacttgt
    13561 atcgaaccaa accgataatc acaccaccat agcccaagct ttcagctgcc aacaaggcgt
    13621 tttgtccagc aagagctgca tcgaccgaac taatcaagag accttccaca ccttggggtt
    13681 ggaaggtgtc ggtatgaagt cgggctccct tttctgctcg gttcaaatct ccgacaaaga
    13741 gaaggaaaac agcagactgg cgaatggctt cttgaggtac caattcatac aaggcatctt
    13801 tcttctcttg acttcgtacc acaatcacag agtaggattg gaaattcttc caagatgatg
    13861 ccatcttggc tgctgtcaaa atctcattta agtctacttg gggaatttct tgctctttaa
    13921 acctgcgcac tgaagtatga gccttcatca atttaatggt ttctgtcatc gacggtttac
    13981 tccttctaaa cgagtctcct cagccaaata acggatgcgt tccatgaccc gtctggcttc
    14041 ccaggtttcg tcatttccat gtttcacttt cgcaaaatgc ttctccaaat cttcaaagtt
    14101 gaagttggat gtgaaaaagg tcggtaaatt ttcctgcatc cgatattgga gaatgacctg
    14161 caggatttcg tcacgcaccc aagcggttga ttgctcggcg ccaatatcat ctaaaatcag
    14221 gacctcagac agcttaatct catccaccaa ggtcttaaca ttgccatcac tgatagcatt
    14281 tttgacatca atgacaaagc taggatagtg gaggagagtt gatgaaacac cacgtttttc
    14341 tgataaatca tgagctaagg ccgccaccat gaaactttta cccacaccaa agtctccata
    14401 taagtaaaga ccttttcgaa tagctggata ttgctccaca aaggctaata gtttttcaaa
    14461 aactggcaag cgtcccaaat catccaaatc aacttgagcc aaactagctt ttttgagact
    14521 ggctggtaga ttgattagct tgagacgatt cttaatagcc gcttcttttt cagccgcgat
    14581 tagctcagga gtttcttcat atgaaacatc tgcatagcca tgattcttaa ccaaaatcgg
    14641 cttgtagcct ttggcaatat aatccgtatc cccacggaga aacttgtcac gctcggtgat
    14701 gtactgatta aacttggaga tactgcgatt taattcctct ggagtcaagg attcttgctg
    14761 gataaaagcc gcaacatcag ggtccttcat gattttctgg accaaatctt gataataaaa
    14821 acggctgggt tgacgtttga gtacgtctcc gacactttcc atctaatctc ctcctttttc
    14881 taatcgagct aatagttctt gtttcttacg ttctagttcc agacgagttt cctcgctggt
    14941 ttcattctta taatctgggt tactccattt aggaatattg gtttttgctg gggcagtctg
    15001 attctgtttt tgtgtttttg ttttctgccc tcgctcacga attcgtaaaa cggcctcttc
    15061 tgctgaatgt atcttttgat aggcatagtc attagccacc ttcatggcat atttctcatt
    15121 gatgtttgcc gaatccacct tattaaaggt caacaagaga atgatattga tgacttcgtc
    15181 cagcaagccc aagccagcca tctgttgcaa aagttctctt tctgtttggg taatggtccc
    15241 cttgcgtgtt tgcttgattt ctgctaagaa ctgcagggca gtcttacttt tagcttcctt
    15301 gataatggtt gcttccttag gattaaagtc agaggaaact ggtttttgag caattttttc
    15361 acgcatgcgt ttggttgaaa taacctgaga aacagctgtt gacttggcca attgataggt
    15421 ttcaaaccaa gtccatttct tctcctcggc aatagcaaag aggtttaaga catcggactg
    15481 ctcatccgca aaacgaagtc catctcgagc catcagctgg cgaaaatgtt ccaagtcaaa
    15541 atcattggcc actttcttct tgagaccaag gtcttcttga ctgcctagtt ctgccaattc
    15601 tggaaagact tgattgagtg agacaggtat ttcttcacca tcagcacttt caactttcaa
    15661 atcctccaca gcagagtcgc caatcttttt ctctaagagt ctgcgataaa caggatgctc
    15721 caagaagtct tgactagata gaggagcatg gagggctagc tgataaacat cccccttttg
    15781 atagagggtc aagagattaa aagcagataa gattttcaat gattttatca gtctatccat
    15841 cccaaagttg agatgattga ggatacttga aaaaagatat tcctttctac cattatccca
    15901 aaaactgatt gtataaagat aaaggctcag tgcctcctga ccgataatcg ggaggtagca
    15961 ctgtaccaga gatgaggtat cttgcgacac ccgattattc tttagataag aaaaacggtc
    16021 aattggcttc atttatcttt cctttttctt tttagaggac tgggtgattt gttggagcaa
    16081 gctctctaac tcactgacat ccttaaaact acgatagaca ctagcaaaac gtacataggt
    16141 aatctcgtcc aattcagcca actcctccat gacgagtgaa ccaatgtcct cactttgaat
    16201 ttcattttca tttcgaccac ggagtttctg ttcgatacga ttgactacca tgttgatttc
    16261 atcacttgac acaggacgtt tctgggctga gcggataatc ccattaaaga ttttatctct
    16321 ggagaattgt tcccgtgtac catctttttt aacaaccact aaggttcttt cttctactcg
    16381 ttcgtaggtt gtaaaacggt gttggcattc gtcgcactca cgtcttctac gaatggtgtt
    16441 cccttcttct gcttggcgac tatcgataac acttgacttg gtagccccac attttggaca
    16501 acgcatcctt tccctcctta tcgttttctt ttcattatac cattttttaa gcgattccca
    16561 aaacaattct tctttttgct tgacaagttt tttgttttgt tgtattattt agttaagaca
    16621 aaaggataag agaaaggaga ctaagatgtc ctggacattt gacaacaaaa aacccatcta
    16681 tttacagatt atggagaaaa tcaagcttca gattgtttcc catacactgg aacccaatca
    16741 acaacttcca accgtgaggg agctagctag cgaggctggt gtcaatccca ataccatcca
    16801 aagagcctta tcagaccttg aacgagaagg atttgtctac agcaagcgaa caactggacg
    16861 atttgtgact aaggataagg agctaatcgc tcagtcacgc aaacaattat cagaagaaga
    16921 attggaacac ttcgtttcct ccatgatcca ttttggctat gaaaaagaag aactaccagg
    16981 cgtagtcagt gattatatta aaggagttta agcctatgtc attactagta tttgaaaatg
    17041 tatccaaatc atatggagca acaccagccc ttgaaaatgt ttctcttgac attccagctg
    17101 gaaaaattgt cggccttctt gggccaaacg gctcaggaaa aacaaccctg attaaactaa
    17161 ttaatggcct cttacaacca gatcaaggac gtgtcctcat caacgacatg gacccaagcc
    17221 cagcaaccaa ggccgttgta gcttatttgc ctgatacgac ctatctcaat gagcaaatga
    17281 aggtcaaaga agccctaacc tacttcaaga ccttctataa agatttcaat cttgaacgcg
    17341 ctcatcatct acttgcagac ctgggcattg atgaaaatag tcgtctcaag aaactatcaa
    17401 aaggaaacaa agaaaaggtt caactgattt tggttatgag ccgtgatgct cgtctctatg
    17461 ttttggatga acccattggt ggggtggatc cagcagcccg tgcttatatc ctcaatacca
    17521 ttatcaacaa ctactcacca acttctaccg ttttgatttc tacccacttg atttctgata
    17581 tcgagccaat cttggatgaa attgtcttcc taaaagacgg aaaagtcgtc cgtcaaggaa
    17641 atgtagatga tattcgctac gagtcaggtg aatccattga ccaactcttc cgtcaggaat
    17701 ttaaggccta agcaaaggag attatttatg ttttggaatt tagttcacta cgaatttaaa
    17761 aatgttaaca agtggtattt agccctctac gcagccgtgc tagtcctttc tgccctcatc
    17821 ggaatacaga cacaaggctt taaaaatcta ccttaccaag aaagtcaggc tactatgcta
    17881 ctttttctag ctacagtctt tggtggcttg atgcttacac ttgcgatttc aaccattttc
    17941 ttgattatta aacgcttcaa aggtagtgtc tacgaccgac aaggctatct gactttgacc
    18001 ttgccagttt ctgaacacca tatcatcaca gccaaactaa tcggtgcctt tatctggtca
    18061 ttgattagca ccgctgtatt ggctctaagt gctgttatta ttctggcttt aacagctcca
    18121 gaatggattc ctctttctta tgtgattaca tttgtagaaa cacatctccc tcagatcttt
    18181 cttacaggta tatccttcct actaaatacc atttcaggaa tcctctgcat ctacctggct
    18241 atttccattg gacagctttt caatgaatac cgtacagcac tcgctgttgc agcctacatt
    18301 ggtatccaaa tcgtcattgg atttattgaa cttttcttca atcttagttc taatttctat
    18361 gtcaattcac tggtaggact caatgaccat ttctatatgg gagcaggtat agccattgtt
    18421 aaagaactca tattcatagc tatcttttat ctcggaacct actacatctt gagaaataag
    18481 gttaatttgc tttaaataat ttttacctag atatgtaaca tactcataga acaaaagaga
    18541 ccaggcaaaa agtctttaaa attagaaaac gcatagtatc aagtgttaaa aatccttgat
    18601 attatgcgtt ttttgtgagc tgacttattt cctttcacta tatcgcaaaa tgaaataaga
    18661 acggaacgat gggattttgg aattcaaatc aatttataag aatgttttag aagtaatatt
    18721 atcctattcc agattcagtt cactatacaa ttgagttttc aagcaacctg tttacataat
    18781 gtgtacataa ttaggttcgt gattccatcc ttttcatctt taaaaacctc gctttcgcaa
    18841 ggctcttcta tttataagat aaggcacgtt taaaggtttt ccaaatccct aaatcatccg
    18901 tttgaagaac gagactagca tacatgcgtc cgataaatcc tgttgctacc accgcaaaaa
    18961 tcactgtaat agcaagtgaa atccatgctt ctgctccccc cgcatagcca ttaatcgttc
    19021 gaaacggcat aaagaaggtc gaaataaagg gaatataaga accaatcttc aagaggagat
    19081 tgtcaccagc tgcacctaga gctgtcactc caaaaaaacc acccataatc aaaatcatca
    19141 aaggcgacaa ggctttccct gagtcctcag gacgagaaac catagatcct aggaatgctg
    19201 ccaagactac gtacatgaaa agactgatca aaataaagag caaggtattc agtgagatag
    19261 catctcccaa gtgatccaaa ataccagact gagccaagaa tggcaaatct ttaaagagca
    19321 aaacggcagc cagaccacct acaacataga tcccaatatg cgttaaaatc actagaaaca
    19381 gagccatcat ccgcgcatag aaatagtgac ttgcccttat gctagaaaaa acgacttcca
    19441 taattttggt gcctttttca ctggcaactt cctgagctgt tacacccgca taggtaatca
    19501 gaatcatata aagaaagaat cctaaggcac ctgctgcaat tgtttgaata aactttttat
    19561 tttccttggc ttcatcaatc ttttctgtga attgaattgt ctgctctaag cgtttttcct
    19621 gctcttgaga caaggaagca gttgaacgat taagctgatt ttgcagttca ttgagtgtac
    19681 ctgtaacctc aaatttaatt ccattttcaa gcgatgtttc gccatgataa actgccttta
    19741 gaacactatc ttcttgatca atggtcaaat aaccttttaa tttttcttct ttaattgctt
    19801 ctttggcact tgcttcgtct ttatagtcga agttaacacc atttacattc ttcagtcctt
    19861 ctgctacaga tggcactgtt gtcactactg ccactttatt atttttagcc atagaagaac
    19921 cttggagatg cccaattcct acagagattc ctaaaaagag gaacggcgaa atcaccataa
    19981 agaagaaact ccatgactcg acatgtcgaa gataggtttc cttgattaca acccacatat
    20041 ttctcatact tccactcctg attctagttt aaagatttca tcgatagttg gcgcttgttg
    20101 gtcaaatgtt gcgatatatt gaccttgagt caagattggg aagagttccc ttccagcgct
    20161 ctcatcctcc aaaatcaatt tccaactgcc ttgtttggtc aagctcacct gtttgacatg
    20221 aggaagattt tccaattctt ccttgcttcg ttcacttgaa acaaagagac gcgttttccc
    20281 gtattgattg cggacatcct gaactggtcc gtgcaagacc acacggccat ctcggatcat
    20341 cagaatatcg tcacaaagtt cctcaacatt ggtcatgaca tggtcagaaa agataatggt
    20401 tgctccgcgc tctttttcct gaaaaatgac ttgtttgagc aattctgtat taactgggtc
    20461 caatccacta aaaggctcat ccaagataat caggtctggt tcatgaatca gagtaataat
    20521 gagctgaatc ttctgctgat ttccttttga cagactcttg attttatctg tcagctttcc
    20581 tttcacttcc aacctcttca tccattgagg gagtttttct ttgacttctt tggcatccat
    20641 gccttttaga gtcgccaagt agcgaacttg ttcaagaact gtcaatttag gcatgagact
    20701 gcgttcttca ggcagataac caatccgagc ataggtctcc tgacgaatat cctgaccatc
    20761 cagaccgatt tctccctgat attctaggaa tttcaaaata ctatggaaaa tcgttgtttt
    20821 tccagcacca ttttttccga ctagtcccaa aatacgacct ggtcgcgctt gaaagtcaat
    20881 accaaacaaa acttgcttgg agccaaaact tttctctaga cttcttactt ctagcatctt
    20941 tcacctccga aatttcttgc actcattata ctcctttttg atagccttta caatgttttt
    21001 gtccattttt agaagactat tgctgtgtaa aatatggcct ggagcacttt tatactcaat
    21061 gaaaagcaaa gagcaaacta ggaagctagc cgtaggctgc tcaaagtaca gctttgaggt
    21121 tgcagataaa actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga
    21181 cgaagtcagt aactatatct acggcaaggc gaagctgacg tggtttgaag agattttcga
    21241 agagtattag tgataaatcc attatacagt agcaaactta atttatacct tccgctcctc
    21301 aactgtctat ttttaatcct gaattgttat ttgagtaact cctttttcct cgtaaagttt
    21361 tcttcctcta aaacttctgg aaaaaggcta atagtttcag acaacatttt tataagaaac
    21421 aagttcatct gtcatttcaa gaaggagtaa tcctttatct actaatggac ggaacggaat
    21481 tcaaccgctt gtccgatatg ttttctaagg attatatagt aaaatgaaat aaaaacagga
    21541 caaattgatc aggacagtca aattgatttc taacaatgtt ttagaagtag atgtatacta
    21601 ttctagtttc aatctgctat atctattatg cacatcacta taggatctaa tgaaaatcac
    21661 aacaggctca ttcatagatg gttacctcag ggaactaaga aaacaactac aaaggaagtc
    21721 gcatttatcg aaaagtggat taacaactat cctaaaaaat gcttgaacta caagtccccc
    21781 agagaagact tctggatgac taacttgaac ttgaaattta gcaataatta actcaccatc
    21841 taactata
//
LOCUS       DAVLYR010000016        18418 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLYR010000016 DAVLYR010000000
VERSION     DAVLYR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.016"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..362)
                     /locus_tag="VYO63_000552"
     CDS             complement(<1..362)
                     /locus_tag="VYO63_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1671525.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIIKKDTHKEIIAKLDYEAPSCP
                     ECGSQMKKYYFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKIMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKISMT"
     gene            complement(517..1227)
                     /gene="treR"
                     /locus_tag="VYO63_000553"
     CDS             complement(517..1227)
                     /gene="treR"
                     /locus_tag="VYO63_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1671526.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            1412..3379
                     /gene="treP"
                     /locus_tag="VYO63_000554"
     CDS             1412..3379
                     /gene="treP"
                     /locus_tag="VYO63_000554"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1671527.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFVYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            3557..5182
                     /gene="treC"
                     /locus_tag="VYO63_000555"
     CDS             3557..5182
                     /gene="treC"
                     /locus_tag="VYO63_000555"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1671528.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQTLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            5438..5686
                     /locus_tag="VYO63_000556"
     CDS             5438..5686
                     /locus_tag="VYO63_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1671529.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            5893..6687
                     /gene="racE"
                     /locus_tag="VYO63_000557"
     CDS             5893..6687
                     /gene="racE"
                     /locus_tag="VYO63_000557"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1671530.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            6684..7655
                     /locus_tag="VYO63_000558"
     CDS             6684..7655
                     /locus_tag="VYO63_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1671531.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            7631..8152
                     /locus_tag="VYO63_000559"
     CDS             7631..8152
                     /locus_tag="VYO63_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1671532.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            8149..8610
                     /locus_tag="VYO63_000560"
     CDS             8149..8610
                     /locus_tag="VYO63_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1671533.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            8601..9341
                     /gene="xerD"
                     /locus_tag="VYO63_000561"
     CDS             8601..9341
                     /gene="xerD"
                     /locus_tag="VYO63_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1671534.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            9341..10069
                     /locus_tag="VYO63_000562"
     CDS             9341..10069
                     /locus_tag="VYO63_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1671535.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            10088..10657
                     /locus_tag="VYO63_000563"
     CDS             10088..10657
                     /locus_tag="VYO63_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1671536.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQALDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            10644..11366
                     /locus_tag="VYO63_000564"
     CDS             10644..11366
                     /locus_tag="VYO63_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1671537.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            11366..11608
                     /gene="yidD"
                     /locus_tag="VYO63_000565"
     CDS             11366..11608
                     /gene="yidD"
                     /locus_tag="VYO63_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1671538.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(11833..12798)
                     /locus_tag="VYO63_000566"
     CDS             complement(11833..12798)
                     /locus_tag="VYO63_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671539.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(12894..13646)
                     /locus_tag="VYO63_000567"
     CDS             complement(12894..13646)
                     /locus_tag="VYO63_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1671540.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            complement(13643..14599)
                     /locus_tag="VYO63_000568"
     CDS             complement(13643..14599)
                     /locus_tag="VYO63_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1671541.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(14589..15548)
                     /locus_tag="VYO63_000569"
     CDS             complement(14589..15548)
                     /locus_tag="VYO63_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671542.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFISSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(15971..16672)
                     /gene="galE"
                     /locus_tag="VYO63_000570"
     CDS             complement(15971..16672)
                     /gene="galE"
                     /locus_tag="VYO63_000570"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1671543.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMDQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            16942..18312
                     /locus_tag="VYO63_000571"
     CDS             16942..18312
                     /locus_tag="VYO63_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1671544.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         5113 a         3657 c         4182 g         5466 t
ORIGIN      
        1 gtcatagaaa tcttttcaat taacttttga gcaatctttt ggttgatgat acgagggatt
       61 tggtgattct tcttgacgat agaagtttca gcaaccataa ttttagaaca atgatagcac
      121 ttgaaacgac gctttctaag gagaattcta gtaggcatac cagttgtttc aagataagga
      181 attttagaag gtttttgaaa gtaatatttc ttcatttgac ttccgcactc aggacaagat
      241 ggagcttcat agtccagttt ggcgatgatt tccttgtgtg tatccttctt gatgatgtct
      301 aaaatctgga tattagggtc tttaatgtct agtaattttg tgataaaatg taattgttcc
      361 atatgattct ttctaatgag ttgttttgtc gcttttcatt ataggtcata tgggactttt
      421 tttctacaca aaataggctc cataatatcc ataggggggt tacccactac aaatattata
      481 gagccgaaaa aggctgagat ctggtgtctc agtcttttat ttcttgcgtt ttgcaaaatc
      541 tacaaatcta aatttttcta acttatggcg actttcggta aactgaaatt ggcgtccgtc
      601 ttggagatag acttttgatt taatcgaaac gacataaggg tctttgccaa tgtccatgag
      661 aatcttgtct cggtcgcttg agtggtcaat ggtgatttcc ttctgagcat aatcaataag
      721 gagtttgagg ccattttcta tataagaata gatagactgc tcagcaattt ggcgagtgag
      781 atttggaatt agttccatat ccagatagtc tgtatccaga acggatacca gatcatccac
      841 cacacgctgg cggaccacct tccaaaccat ccgaaactct gggaaaccgg ttatcagtga
      901 ggatttttta tcaataataa tcttgtccag actgaccacg ttggttttag agcgcagtcc
      961 aagttcttta actagttctt ggtagctggt taggttggat acagggaaat tgacggtttc
     1021 ttctttgacg acttgagaac cttgccctct tatctttttg atcaatcctt cctcttggag
     1081 gagagacagg gcctttcgga cggtatcacg actcacttga tattgctcca taaggtcgtg
     1141 ctcgctagga aggaaatctc cgacagcgta agtctcgttt tgaatggttt cttggatttg
     1201 cttaaataat tgttggtatt tcttcatttt gcctgcttaa ataggttttt agtctagttt
     1261 aatgaacacc tgtatctatt ttaccataca agtgctagaa aatttacaaa aaaacaccct
     1321 ttttatacca aacaagttgc agacaagttt ggtatcgttt acaatatact tatcaaatca
     1381 aacttgcttt gacaagtata aggagaatca aatgggaaaa tttgaacaag aagccaaaga
     1441 tctgcttcag gcaattggag gcaaagaaaa tgtgactgcc gtcacccact gtgcgacacg
     1501 gatgcgcttt gttttaggag atgataagaa ggctaatgtt aaagctatcg agtcaattcc
     1561 agctgttaaa ggaactttta caaatgcagg tcaatttcag gtaatcattg gaaatgacgt
     1621 gcccatcttt tataatgatt ttacagccgt ttcaggtatt gagggtgttt ccaaagaagc
     1681 agccaagtct gcagctaaga gtaatcaaaa cgtggtccaa cgtgttatga ccactctggc
     1741 ggagattttt actccgatta ttccagcctt gatagtcgga ggattgatcc tcggtttccg
     1801 taatgtcttg gaaggtgtcc attggtcgat gttggatggc aagaccatca cagaatcctc
     1861 tcagttttgg gcaggtgtca atcacttcct ctggttgcct ggtgaagcta tcttccagtt
     1921 cttaccagta gggattactt ggtctgtttc tcgtaagatg ggaaccagcc aaattttggg
     1981 aattgttctc ggaatctgtt tggtatcgcc tcagttgctc aatgcctatg cggttgcttc
     2041 aacgccagca gctgatatcg cggcaaactg ggtttggaat tttggctatt ttactgttaa
     2101 tcgtatcggt taccaagccc aagttatccc agccttgctt gcaggtttga gtctgtctta
     2161 tcttgaaatc ttctggcgca agcatatccc agaagtcatt tctatgattt ttgtaccttt
     2221 cttgtcattg attccagcct tgattttggc tcatactgtt ttgggaccaa tcggttggac
     2281 aattggacaa ggactttcat cagttgtctt ggcaggttta actggtccag ttaaatggct
     2341 cttcggtgca atttttggtg ccctctacgc tccatttgtc atcacaggtc tgcaccatat
     2401 gaccaatgcc attgatacac aattgattgc ggatgctggt ggcactgccc tatggccaat
     2461 gattgctctt tctaatattg ctcaaggctc agccgtgttt gtctattatt tcatgcatcg
     2521 ccatgatgag cgtgaggctc aggtttcact tcctgcaacc atttcagcct atctcggtgt
     2581 tacagaacca gctctttttg gggttaacgt aaaatatatt tatccatttg ttgctgggat
     2641 gactggttca gcccttgcag gcatgttatc cgttactttt aatgtaactg cggcttctat
     2701 tggtatcggt ggtttgccag gtattctctc tattcaacct caatacatgc tgccatttgc
     2761 aggaactatg ctagttgcga ttgttgttcc aatgctcttg actttcttct tccgcaaggc
     2821 tggtctcttt acaaaaacag agggcgatac gaacttgcag gcagaattcg ttgctcaaga
     2881 agaagcagaa tttgtgaacc atgaaccagt agaacttact tcggtagaaa ttatcagccc
     2941 actaactggc caagtgaaag aattgagtca agcgacggat cctatttttg catcaggtgt
     3001 catggggcaa ggtctagtca ttgaaccaag ccaaggtgag ttgacctctc cagttaatgg
     3061 gacagtgacg gttcttttcc ctaccaagca tgccatcggc attgtctctg acgagggagt
     3121 tgaattgctc atccacatcg gtatggatac agtaggtctt gatggcaaag gttttgaaag
     3181 tcttgtagtc caaggagatc acgttacagt tggtcagcaa ctgattcgtt ttgatatgga
     3241 tgtcattaag gctgcaggtc tggtgacaga aactcctgtt atcatcacca accaagatgc
     3301 ttatacagcg actattcccg gaacttatcc gacaacgatc caagctggag catctctcat
     3361 ggtcgctaca cgaatctaat actcttcgaa aatctcttca aaccacgtca gctctatctg
     3421 caacctcaaa atagtgtttt gagcaacctg tggctagctt cctagtttgt tctttgattt
     3481 tcattgagta taattgaaat agcacaaata gggacggaag actgcttcct cccttttatc
     3541 agaaaaggag aaacagatga cacttgataa aggaaaagta gtctatcaaa tctatccaaa
     3601 atcttacaaa gacaccactg aaaacggtat tggggatttc cgtgggatta tcgaaaaaat
     3661 tccctattta gccaaactag gtgtggatat ggtctggctc aatccattct atccaagccc
     3721 tcaacgggat aatggttacg atatttcaga ttatatggca gtggatcctc tttttggtga
     3781 tatggctgat tttgaggaaa tggtgtgtgt cggtaaagag cacaagattg actttatgct
     3841 ggacatggta cttaatcatt gttcgacaga gcatgaatgg tttcagaaag ccctagctgg
     3901 tgataagtat tatcaagact ttttcttcat ccaagaccaa ccaacagatt ggcagtctaa
     3961 gtttggtggt tctgcatgga cgcctttcgg ggatactggg aaatactatc ttcacctatt
     4021 tgatgagacc caggctgacc ttaactggcg caattccaat gtccgtaagg agcttttcaa
     4081 ggttgttaat ttctggcgcg acaagggtat caaaggtttc cgatttgatg tgatcaattt
     4141 gatcggtaag gacgaggttt cagtggactg tcctgaaaat gaagggaagc cagcttatac
     4201 agacaaaccc atcgttcata actatttgcg tatgatgaac caagccactt ttggatctga
     4261 cgatagcttt atgacagttg gggaaatgtc ttctaccacc atggaaaact gtgtcctcta
     4321 ttcgtcgcct gatcgtcagg aattatccat gacctttaat tttcatcacc tcaaggtgga
     4381 ctataaggat ggacaaaagt ggaccttggc tccctttgat tttgaagagt tgaagagtct
     4441 ttaccatagt tggggtaagg aaatgagtga taaagacggt tggagtgccc tcttttggaa
     4501 caatcacgat caaccacgtg ccttgaaccg ttttgtcgat attcaaaact tccgcaagga
     4561 aggagccacc atgctagcgg ctagcattca cctgtcacgt gggacacctt atatctatat
     4621 gggtgaagaa atcgggatga ttgacccaga ctatgattct atggctgatt atgtggatgt
     4681 cgaatcgctt aatgcctatc agacgctctt agaagaagga aagagtcagc aagaagcctt
     4741 ccagattatt caagctaagt cgcgtgataa ttcacgcatt cccatgcagt gggatgcttc
     4801 ggaaaatgca ggattttcaa caggcactcc atggttgaaa gctggtaaat cctataaata
     4861 catcaatgta gaaaatgaaa tccagggtcc gattttcacc ttctatcaag acttgattcg
     4921 actgcgtaag gaaatgccaa tcatttcaga aggaagttac aaaccagcct ttgaagatag
     4981 caaacaagtc tacgcttttg aacgccagtt tgaggatcaa aagttactag tgctcaataa
     5041 cttttatgct aaagaagtgg aaatcgactt gccagcagtc taccaaaatg gacagatttt
     5101 gatatcaaac tatgaagatg cagaagtgtc tgagaaaatt ctactcaaac cctatcaaac
     5161 actggctatt tatgtaaatt aatactcttc gaaaatctct tcaaaccgtg tcagttttat
     5221 ctgcaacctc aaagcggtgc tttgagcaac ctgtggctag cttcctagtt tgctctttga
     5281 ttttcattga gtataaactt agcaaggaga cctctttttc agaaaagcag ggttcttttt
     5341 gctgtctatt ccatccctga aagtgtagaa caaactggtc agggtggcta gtttgtggta
     5401 taatgaaagg gattcaaaaa gaaacaggag aaaaatgatg gatttacttt tagcaattgt
     5461 attgattgtg ctagcttttc taggaggagc tcttggagga atgtacttgg ttcgtaagca
     5521 aatcgaaaaa gaatttgctg acaacccacg tttgaatgct gaagcagttc gtactctttt
     5581 gagtgcaaat ggtcaaaaac caagcgaagc taaggtacaa caagtttacc accaaatcat
     5641 ccgccaacaa aaggcagccc ttgctaacaa taaaaagaaa aaataaataa ggaaaagtct
     5701 gggatgaaag ttccagactt ctcactatgt tggttatggt ttagggatga gactttttcc
     5761 ttgcattcta ttgatatttg attatgatta agagagaggt agttgacatt ctatcaagct
     5821 gtcggtcagt tcgttcagta caaggaatca taatgaacga tcaatcagaa aaaagactag
     5881 aaagaagact gtatggataa tcgaccaatt ggttttttgg attcgggtgt cgggggcttg
     5941 accgttgtgc gcgagctcat gcgccagctt ccccatgaag aaatcgtcta tattggagat
     6001 tcggcgcggg cgccctatgg cccccgtcct gctgagcaaa ttcgtgaata tacttggcag
     6061 ctggtcaact ttctcttgac caaggatgtc aaaatgattg tcattgcttg taacactgcg
     6121 actgcggtcg tctgggaaga aatcaaggct caactagata ttcctgtctt gggtgtaatt
     6181 ttgccaggag cttcggcagc catcaagtcc agtcaaggtg ggaaaatcgg agtgattgga
     6241 acgcccatga cggtacaatc agacatatac cgtcagaaaa tccatgatct ggatcccgac
     6301 ttacaggtgg agagcttggc ctgtcccaag tttgctccct tggttgagtc aggtgccctg
     6361 tcaaccagtg ttaccaagaa ggtggtctat gaaaccctgc gtcccttggt tggaaaggtg
     6421 gatagcctga ttttgggctg tactcattat ccactccttc gccctattat ccaaaatgtg
     6481 atggggccaa aggttcagct catcgatagt ggggcagagt gcgtacggga tatctcagtc
     6541 ttactcaatt attttgaaat caatcgtggt cgcgatgctg gaccactcca tcaccgtttt
     6601 tacacaacag ccagtagcca aagttttgca caaattggtg aagaatggct ggaaaaagag
     6661 attcatgtgg agcatgtaga attatgacaa ataaaattta tgaatataag gatgaccagg
     6721 actggtatgt tgggtcttat agtatttttg gtggcgttaa cagtttgagc gactatcaga
     6781 cagattttcc tctgtttgaa ttctccaaaa tatttggaga tgaagagtat ggtttcccgc
     6841 tttcagttac tgttttacgc tatggttcta cctaccgttt gttctccttt gtggtagata
     6901 tgcttaatca agaaatggga cgaaacttgg aagttattca acgtcatggg gccctgctct
     6961 tggttgaaaa tgggcaactc ttgtatgtag aattgcctaa agaaggggtc aatgttcatg
     7021 atttctttga gacaagcaaa gtcagagaaa ccttgttgat tgcgactcgt aacgaaggta
     7081 aaaccaagga attccgagct atctttgata agttaggcta cgatgtggaa aatcttaatg
     7141 actaccctga cctgcctgaa gtagcagaaa caggtatgac ctttgaggaa aatgcccgcc
     7201 ttaaggcaga aaccatttct caattaacgg gcaagatggt tttggcagat gattctggtc
     7261 tcaaagtcga tgtccttggt ggcttaccag gcgtctggtc agctcgtttc gcaggtgtgg
     7321 gagcaactga ccgtgaaaat aatgccaaac tcttgcacga attggccatg gtctttgaac
     7381 tcaaggaccg ctcggctcag ttccacacaa ccctagtcgt agccagccca aataaggaaa
     7441 gtttagttgt tgaagcagac tggtcaggtt atattaactt tgaacctaag ggtgaaaatg
     7501 gctttggcta tgatcccctc ttccttgtag gagagacagg tgagtcatca gctgaattaa
     7561 ccctggaaga aaaaaatagt caatctcacc gtgccttagc cgttaagaaa cttttggagg
     7621 tatttccatc atggcaaagc aaaccatcat tgtaatgagc gattcccatg gcgatagctt
     7681 gattgtggaa gaagtccgtg atcgctatgt gggcaaagtc gatgccgttt ttcataacgg
     7741 cgattctgaa ctacgtccgg attctccact ttgggagggc atccgcgttg ttaaagggaa
     7801 catggacttc tacgccggct acccagaacg tctggtgact gagcttggtt cgaccaagat
     7861 tatccaaact catggtcact tgtttgacat caatttcaac tttcaaaagt tggactactg
     7921 ggctcaggag gaagaggccg ctatctgcct ctatggtcac ttgcatgtgc caagtgcttg
     7981 gttggaaggc aagatcctct ttctaaatcc aggctctatc agtcaaccac gaggtaccat
     8041 cagagaatgt ctctatgctc gtgtggagat tgatgatagt tacttcaaag tggacttttt
     8101 gacacgagat cacgaggtgt atccaggttt gtccaaggag tttagccgat gattgccaag
     8161 gagtttgaga ctttcttgtt ggggcaggag gaaacttttt tgacccctgc taaaaatcta
     8221 gctgtgttga ttgatactca caatgcggat catgcgaccc tcttgctcag tcagatgacc
     8281 tatacccgtg ttcccgttgt gacagatgaa aaacagtttg ttgggacgat tggactcaga
     8341 gatattatgg cttatcagat ggagcatgac ttgagccaag aaatcatggc ggatacggat
     8401 atcgttcata tgacaaaaac ggacgtagcg gttgtttcgc ctgatttcac cattacggag
     8461 gtcttgcaca agctagtaga tgagtccttc ttaccggttg tggatgcaga gggtattttc
     8521 caagggatta ttacgcgcaa gtccatcctc aaggccgtta atgccctctt gcatgacttt
     8581 agtaaggaat atgagattcg atgccaatga gagacaggat ttcagccttt ttagaggaaa
     8641 agcagggctt gtctgtcaat tccaagcagt cctataagta tgatttggag caatttttag
     8701 acatggtagg tgagcggatt tctgagacca gtctcaagat ttaccaagcc cagctagcca
     8761 atctaaaaat cagcgcccag aagcgaaaga tttcggcctg taaccaattt ctatactttc
     8821 tctatcaaaa aggagaggtg gacagctttt accgcttgga attagccaaa caagctgaaa
     8881 agaagacgga aaagccagag attctatacc tagactcttt ttggcaggaa agcgaccatc
     8941 cagagggccg cttgctagcg ctcttaatcc tagaaatggg gctcttgccc agtgagattt
     9001 tagccatcaa ggttgcggac atcaatctgg attttcaggt gttgcgaatc agcaaggctt
     9061 cccaacggag gattgtcacc attcccacgg ccttgctttc agaattggaa cccttgatgg
     9121 ggcagaccta tctttttgaa agaggaggga aaccctattc tcgtcagtgg gcctttcgtc
     9181 agttagaatc ttttgtcaag gagaaaggtt ttccatcctt atcagctcaa gtcttacgtg
     9241 aacagtttat tctaagacaa atagaaaaca aggtcgattt gtacgaaatt gcaaaaaaat
     9301 taggattaaa aacagtcctg accttagaaa aatatagata atggatatta aattaaaaga
     9361 ttttgaagga cccctggact tactcttgca tctggtttct aagtaccaga tggatatcta
     9421 cgatgtgccc attacggaag tcatcgaaca gtatctagcc tatgtctcaa ccctgcaggc
     9481 catgcgtctg gaagtgacgg gtgagtacat ggtcatggct agtcagctca tgctgattaa
     9541 gagtcgtaaa ctccttccga aggtagcaga agtgacagac ttgggggatg acctggagca
     9601 ggacctcctc tctcaaatcg aagaatatcg caagttcaag ctcttgggtg agcacttgga
     9661 agccaagcac caagaacggg cccagtatta ttccaaagcg ccgacagagt tgatttacga
     9721 agatgcggag cttgtgcatg acaagacgac cattgacctc tttttggctt tttcaaatat
     9781 cctagccaag aaaaaagagg agtttgcaca aaatcacacg actatcttgc gggatgagta
     9841 taagattgag gacatgatga ttattgtgaa agaatccttg attggacgag atcaattgcg
     9901 cttgcaggat ttgttcaagg aagcccagaa tgtccaagag gtcatcaccc tctttttggc
     9961 aaccctagag ttaatcaaaa cccaggagct gatcctcgtg caagaggaga gttttggaga
    10021 tatctatctc atggaaaaga aggaagaaag tcaagtgcct caaagctaga cttgatagag
    10081 aggaaagatg agtactttag caaaaataga agcgctcttg tttgtagcgg gtgaagatgg
    10141 gattcgggtc cgccagttag ctgaactcct ctctctgcca ccgacaggca tccagcaaag
    10201 tttaggaaaa ttagcccaga agtatgaaaa ggacccagat tccagtttgg ctttgattga
    10261 gacaagtggt gcttatagat tggtgaccaa gcctcaattt gcagagattt tgaaggaata
    10321 ctctaaggcg cctatcaacc agagcttgtc tcgggctgcc cttgagacct tgtccattat
    10381 tgcctacaaa cagccgatta cgcggataga aattgatgcc atccgtgggg ttaactcgag
    10441 tggagccttg gcaaagttgc aagctttgga cctgataaag gaagacggga aaaaggaagt
    10501 attggggcgc cccaacctct atgtgactac ggattatttc ctagattaca tggggataaa
    10561 ccatttagaa gaattaccag tgattgatga gcttgagatt caagcccaag aaagccaatt
    10621 atttggtgaa aggatagaag aagatgagaa tcaataagta tattgcccac gcaggtgtgg
    10681 ccagtaggag aaaagcagaa gagctgatca agcaaggttt ggtgacagtt aacggccaag
    10741 tggtgcgtga actagcaacc actatcaagt caggcgacaa ggtcgaagtt gaaggtcaac
    10801 ctatctacaa cgaagaaaag gtctattatc tgcttaacaa accacgcggt gtcatttcca
    10861 gtgtaacaga tgacaagggt cgcaagacgg ttgtcgacct cttgcccaat gtcaaagagc
    10921 gcatttaccc tgtgggtcgt ttggactggg atacatcagg agtcttgatt ttgaccaatg
    10981 atggggactt tacagacgag atgattcacc ctcgtaatga gattgacaag gtttatgtcg
    11041 cgcgtgttaa aggtgtggcc aataaggaca atcttcgccc cttgacccgt ggtcttgaga
    11101 ttgatggtaa gaaaaccaag ccagctgttt atgaaattct caaagtggac ccagtcaaaa
    11161 atcgctctgt ggtgcagttg accatccatg aagggcgtaa ccatcaggtt aaaaagatgt
    11221 ttgaagctgt tggtctccaa gtagataagt tgtctcggac tcgtttcgga cacctagact
    11281 tgacaggact ccgtccagga gaatcccgtc gtcttaataa aaaagaaatc agccaactac
    11341 acaccatggc tgtaactaag aaataatgaa acgaatttta atagcgcttg tgcgctttta
    11401 ccaacgtttt atctcaccag tctttccacc ctcttgtcgc tttgagctga cttgttccaa
    11461 ctacatgatt caggctattg aaaaacatgg ttttaagggg gtattgatgg gcttggctcg
    11521 gattttacgt tgtcatccct ggtcgaaaac aggtaaggac cccgttccag accacttttc
    11581 ccttaaacga aatcaagaag gggaatgagg tggggtaaat agatttcaaa atgataaaaa
    11641 cgcatcctat caggtttgag tgaacttgat aggatgcgtt ttagaatgtc aaaattttat
    11701 actcttcgaa aatctcttca aaccgcatca gctttcatct gcaacctcaa aacagtgttt
    11761 tgagcaacct gcggctagtt tcctagtttg ctctttgatt ttcattgagt attaaattga
    11821 gtttgaagtg gcttatttca aagctttttg tatgtcttca atcatgagtt ttgttgattc
    11881 aagtccgcct ccgcttagat accagaggtc tggtgttagt tggataatct taccattttt
    11941 agcagcaggt gtttcagcga taagggcatt ttctaggaca ccgtcgttgc tagagttgtc
    12001 cccaccgatg gcaagggtac ggttgatgac aaagaggatg tcagggttga tttctttgac
    12061 actttcaaag ctgacttctt gtccgtggcg tgagtcttca aattttgtat cagttggttt
    12121 gaatttcaag gtttggtaca agaaagagaa acgagatttg gcaccaaagg ctgccatttt
    12181 tccttcatta aggaggatcg caagggcttt tttgtcagag ctttcatttt tagtagcgac
    12241 ttcttggatg ctcttgtcta gcttggccaa ttcttccttg gctttttgtg tactagtttc
    12301 gccgaaggca cttgctaagg attcgatatt agccttggta gaagtccagt agtcatcctt
    12361 gcttgcttgg aagagaacgg ttggggcgat ttctttgaat ttgtctacaa atttttgtgt
    12421 acgtggtgaa gcgataatca aatcaggctc aagggcggcg atagcttcta aatcaggttc
    12481 tttcatagaa ccaacatttt tgacagttcc cactaggtct tttagataag tcggaacagt
    12541 ttttgtaggc attccgacga tatttttttc aaatcctaaa gcgcgaatag tatccgcagc
    12601 gccgaggtca aaggtcacaa tcttttcagg aactttggaa agtttgacct cgtccagtga
    12661 acttttaatg gttacctctg ttggagcaga gctactggtc tctgtctgac tagtgcttga
    12721 gtttgtacta catgcaccaa gtaggagcaa gaagctggcc actagggcag tgaaataaag
    12781 tttaagggat gttttcataa tttctccttt ttaaaatgtg ataacgattt agggagtctc
    12841 ttaatcttat tgactaagag actgaaggtt ctctaacttg agcttttatg ttactagcta
    12901 tagatacaga tctttttgtt attgatatca gctagcgtga tgggaatctc ataaagttga
    12961 ctgagcaggt cagcctgcat gatttgatcg gttcttccct tgctaaagac ctggccgtcc
    13021 ttgaaggcga caatttcatc tgcatactga ctggccatgt tgatatcgtg gaggacgatg
    13081 ataatggtct tgccgagttc ctccaccagt cgtcgaagaa tctgcatcat gctgacgctt
    13141 tgcttgatat cgagattgtt gagtggttcg tccagcaaga taaagtccgt atcctgggcc
    13201 agtaccatag cgataaagac gcgctggagt tgcccccctg acaggctatt gatgtagcgg
    13261 tcttttaagt tggtcagttc caaatagttc agagtttctc ggattttttc ccagtcttct
    13321 gatctaagtc gacctcggct gtagggaaaa cgtccaaaac tgaccagttc ttcaacagtc
    13381 aatttggctt ggtaattgat tttctgtttt aggatggtta gttcttgggc cagttcttgc
    13441 gaattccagc tctcgatttc acgtcctttg atactgagaa ctccctgatc tttcttggtt
    13501 agtctgctca tgatggagag gagagtcgat tttccagcac catttggacc aataaaggct
    13561 gtcagttttt gaggactgac ttcaagcgaa atgccttgca aaatatcctg tttttgaatg
    13621 gatttgtcaa tgttttccag tttcactgac gagccctcct atatagtaag ataaagaata
    13681 agaagccacc cacactctca atgatcatac tgatacgaat ttccagtgca aagactcgtt
    13741 caatcaaggc ttgccccaag gttaagctaa taaatccaac cagaatggcc actataaaga
    13801 gtaacttgtg ctgatagtct ttgacaatca ggtaggtgag gttggccagc ataaagccga
    13861 agaaggccat aggtcctacc aaggcagtgg ccgttgaggt caaaagcacg attccccaga
    13921 ggagctcttt ctgttctttt tcaacatcga gtcccaatat ctgagccgtt tctctttgca
    13981 ggtgcaagac atctagaacg actgcttttc gaaagaaaaa gattgtcaaa gcgaggatga
    14041 tcagagaacc gatggctagg atggaagtgt taagatgttg aaaggaggca aaaagactat
    14101 tttgcagttt atcgtattcg tttggatcca ttaggacttg aaggaaggtg ctgatatttc
    14161 gaaagagact tctgagcgct agacagatca gcaggatgaa gaccaggtct tgcttcatca
    14221 gtgtcttcaa gtaaccttgt aaggcgagaa agaagaggga ctggacaaga agtaagacta
    14281 ggaattctaa gataggggat ttgccaagtt gaagaaactt gctttcaaaa accagtagta
    14341 gggtttgtag taggacgtag aaggattcaa ttcccaaaat actaggcgtc aggaagcgat
    14401 tttccgtcag ggtttgaaaa ctaatggtcg aaatcccagt cgcgatggct accaagagat
    14461 aaacgatgat cttttgggaa cgcaacttcc aagcaaaggc tgacaagtga gtgatgggcc
    14521 aaaagtagag aagacaagct ccgatggcaa gaataatgag aatccagaag agcttggtat
    14581 gtttgctttt agtctgcatc ttttcgtccc cctctccaga gaagtaggat aaagacgaga
    14641 ctaccgatga ttcctagcaa gagactgaca gacaactcat agggcctaat cagaactcgg
    14701 gataggatat cgcaagccag aactagattg gcaccaacca gtgcgaccat gagtttggtt
    14761 tgacttagat tatctccata gcgcttgcga acaagattgg gaacgataac tccgagaaat
    14821 ggtaagccac ccacggtaat catggtgacg cttgtcgtta gcgccaccag aaagagggcc
    14881 agtttttcaa gtagggagta ggaaatcccc aaactctcgc tggtttcttt ccctagattc
    14941 atgatggtga aggtttggga taatttccaa acggttatca ggatgatgag gcctaagaag
    15001 agccactcat actgatgggt ctgaatcatg gagaaggagc cctgggtcca ggcagtcata
    15061 ctctgaacca gattgaaacg ataggcgata acttctgtga ctgagccgat aatcccgcta
    15121 tagatgatcc caatcagagg caacatccac ctttccttta cagtaaaaat ggtcataaag
    15181 gctaggaaga agagggtgaa tacgatggat gaaataaaag cgaagagcat cttttgggtc
    15241 agactagccg atggaaagac aaaaaggctc agcaccattc ccagtttggc ggcttcagtc
    15301 gttccaactg tactcggtgc agcaaactga ttttgggtaa tagtctgcat gagaaggcct
    15361 gccatactca tactagaggc agtcaggaga atactgatag ttcttgggag acgggactct
    15421 tgaaagagga gccaggtctg ctggtcgaaa tcaaatagct ttccccatga aaaatcactg
    15481 gtcccaatgc taatagagag aaagactagg agtagaagta agccaattaa ataatgagaa
    15541 agtttcatac cccgtccttt catgtagatt tggtatcgaa agatacctgc ggatataaat
    15601 gtaacattat ttttctaatc tgtcaataaa atttctgaca atttaataaa tacaacaagg
    15661 agagagcaac aagactttct cctttgttat cctattctaa aatgttttta ccttaatctg
    15721 ataaaataat atcttcgagg gagtagctag ccgtccaatc aagatattgt ttagcttttg
    15781 aagcatctgc taggacactg gctgggtcac tagcacgtcg agcaacaatc tcgtgtggga
    15841 ttttttaatt tagtaattct tcagcagttt taaagatttc tttgatagta tagccttttt
    15901 tagttcctaa gttaaagatt tgagaagaac tgtcttcttg aaataggtag ttcattcctt
    15961 taacatgagc ctatgcaagg tccaagacat aaatgtaatc tcgaatacat gaaccgtcac
    16021 gtgtatcgta gtcatctcca aatattttta agctatcatg ttgtcccaat gcggtcttgt
    16081 tgatatttgg aatgatgtga gttggatttt tcacacgcag accgtttgaa gcatccattt
    16141 cagccccagc aacattaaag taacggaaaa taacatattt ccagtcgtag cgattggcca
    16201 tccagtaaat cattcgttcg cccatcagtt ttgtctctgc ataagggttg acagggtcga
    16261 gcagggtatc ttcagtcacc ggcttgtcaa tacagttatt tccatagaga gaagcagtcg
    16321 aagagaacat gattttttga atgccaactt cagataagac tttgagaact tggtccatac
    16381 cagcaacgtt ggcagtgaag tatttacttg gattttcaat actttcgctc acaacaattt
    16441 cacctgcaca atggaggaca gcatcaatct gattttcttc taagtaagcc tttaaggcgc
    16501 tagcatcata aacatccagt tctttaaagc tagcacgact atccacagcc gctcgatttc
    16561 ctgtggagag attatcgagg acatgcacct gatagccagc atttaaaaga gctttaacgg
    16621 tatgggagcc aatgtagcta gtcccacctg taacaagaat tgttttggtc atgatttttt
    16681 ccttttctag ttttgtacta tagtaaattg aaataagata tgaacaactc tattagaaaa
    16741 gtcaaattaa tttctagaaa tgttttagca gctacagcgt actattccaa actcaaccaa
    16801 ctataataga tcgaaactag aatagtacac atctacttct aaaacattgt taaaaatcga
    16861 tttgactgtc cttatttcat tccgctatat ataagtttgc tgtttcttgt cgctcctctg
    16921 gaaagctgat ataatagctt tatgaataaa aaacgaacag tggacctgat acatggtccg
    16981 attcttccct cgctcttaag cttcaccttt ccaatcttgc tatcaaatat ttttcaacag
    17041 ctctataaca ctgctgatgt cttgattgtt ggacgatttc ttggtcaaga atccttggct
    17101 gcagtaggag cgacgacagc gatttttgac ctgattgtag gctttacact tggtgttggc
    17161 aatggcatgg ggattgtcat tgctcgttat tatggggctc ggaatttcac taaaatcaag
    17221 gaagcagtag cagccacctg gattttaggt gctcttttga gcattctagt tatgttgctg
    17281 ggctttcttg gcttgtatcc tctcttgcaa tacttagata ctcctgcaga aattcttcct
    17341 caatcttatc aatatatttc tatgattgtg acctgtgtag gtgtcagctt tgcttataat
    17401 ctttttgcag gcttgttgcg gtctattggt gacagtctag cagccctggg atttctgatt
    17461 ttctctgcct tggttaatgt ggttctggat ctctatttta ttacgcaatt gcatctggga
    17521 gttcaatccg caggacttgc taccattatt tcgcaaggtt tatcagcggt tctctgcttt
    17581 tattatattc gtaaaagtgt gccagaactc ttgccacagt ttaaacattt caaatgggac
    17641 aaaagcttgt acgcggatct cttggagcaa ggtttggcta tgggcttgat gagttcaatt
    17701 gtatctatcg gcagtgtgat tttacagttt tctgttaata catttggtgc agtgattatt
    17761 agtgcccaga cggcagctcg acgcattatg acctttgccc ttcttcctat gaccgctatt
    17821 tctgcatcaa tgacgacctt tgcttctcag aatctaggag ctaagcgacc tgaccgtatt
    17881 gttcaaggtc ttcgaatcgg cagtcgttta agtatatcct gggcagtttt tgtttgtatt
    17941 ttcctctttt ttgccagtcc agctttggtt tccttcttgg ctagttcgac agatggttac
    18001 ttgatagaaa atggaagtct ctatctgcaa atcagttcaa ccttttatcc cattttgagc
    18061 ctcttgttga tttatcgcaa ttgcttgcag ggcttggggc aaaagatcct tcctctagtt
    18121 tctagcttta ttgaactaat cggaaaaatc gtttttgtgg ttttgattat tccttgggca
    18181 ggatataagg gtgttatcct ttgtgaacct cttatctggg ttgccatgac agttcaactg
    18241 tacttctcat tattccgtca tcccttgata aaagaaggca aggcaatctt ggcaaccaaa
    18301 gtgcaatcct agttggattt actgaataaa atccatttcc tctagtgaaa atcgaaaaaa
    18361 cttgtgttct cgtctttagt ttggtgttga aaatagttta acagactttt gacttctt
//
LOCUS       DAVLYR010000017        18129 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLYR010000017 DAVLYR010000000
VERSION     DAVLYR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.017"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(17..952)
                     /locus_tag="VYO63_000572"
     CDS             complement(17..952)
                     /locus_tag="VYO63_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1671545.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(949..1809)
                     /locus_tag="VYO63_000573"
     CDS             complement(949..1809)
                     /locus_tag="VYO63_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1671546.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1917..4079
                     /locus_tag="VYO63_000574"
     CDS             1917..4079
                     /locus_tag="VYO63_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1671547.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4171..5430
                     /locus_tag="VYO63_000575"
     CDS             4171..5430
                     /locus_tag="VYO63_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1671548.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5444..6310
                     /locus_tag="VYO63_000576"
     CDS             5444..6310
                     /locus_tag="VYO63_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1671549.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTRWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6324..7157
                     /locus_tag="VYO63_000577"
     CDS             6324..7157
                     /locus_tag="VYO63_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1671550.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKLTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7158..7238
                     /locus_tag="VYO63_000578"
                     /pseudo
     CDS             7158..7238
                     /locus_tag="VYO63_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7515..8957
                     /gene="gtfA"
                     /locus_tag="VYO63_000579"
     CDS             7515..8957
                     /gene="gtfA"
                     /locus_tag="VYO63_000579"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1671551.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTIEIETPTVHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9095..10288
                     /locus_tag="VYO63_000580"
     CDS             9095..10288
                     /locus_tag="VYO63_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1671552.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10975..12954
                     /locus_tag="VYO63_000581"
     CDS             10975..12954
                     /locus_tag="VYO63_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671553.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13021..14517
                     /locus_tag="VYO63_000582"
     CDS             13021..14517
                     /locus_tag="VYO63_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671554.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14517..15443
                     /locus_tag="VYO63_000583"
     CDS             14517..15443
                     /locus_tag="VYO63_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671555.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15452..16519
                     /locus_tag="VYO63_000584"
     CDS             15452..16519
                     /locus_tag="VYO63_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671556.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16530..17456
                     /locus_tag="VYO63_000585"
     CDS             16530..17456
                     /locus_tag="VYO63_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671557.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            complement(17531..>18129)
                     /locus_tag="VYO63_000586"
     CDS             complement(17531..>18129)
                     /locus_tag="VYO63_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1671558.1"
                     /translation="TVSLPNAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEHKA
                     IKHYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLLLFH
                     FQNKEPEKFFELIEDNLKQVHPIFQTVFKTFLKDKEKIINALQLHYSNAKLEATNNLI
                     KLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         5328 a         3218 c         3823 g         5760 t
ORIGIN      
        1 ttataattgt gttattttac ttccaactat tgagagaaat ttcgccacta tttagccaga
       61 tttcttttcc gttatcacat tgaactaaaa gttttccatt ttctgagatg tctttagcaa
      121 gtcccttgta gtctttttgc tctagtgtga aagtgacttc ttttcctaga atgaatgact
      181 gttttttgta taggtataat agctcttctg ctggtgtttc gaagaaagca cgccagattt
      241 ctatgatcaa ttcattcctt gttataggag ctgtagcttt aaataagctg gcagcttttt
      301 cttttaattc ctgagggaag tctttaatag tgaaattgat acctactcca ataatgatat
      361 ctgtgactaa gccagtttct acagaggtca ttgcttcagt aaggattcct ccaattttat
      421 gattgtttag atagatatca ttgacccatt ttatgtcgac atctattaaa gttaggttct
      481 taatggcttt gtagacagct ccagctacaa gtagtgtgta ggatggtaat ttgtcatagg
      541 ggagatttgg tttaagatgg agtgtcatat aaataccacc ttgtggtgag tagaaggaac
      601 gttgaaaacg gcctcggcct gctgtttgat aggaagctag atagagggta tttgcttcat
      661 ggcctaaatc aattgcttct tttgcatcta gttgtgttga ttttgtttcg ggtttaaagc
      721 tgactttaat tggaagattt tcttctagaa tctctggaag aataaggtca ccattcatca
      781 gtttatatcc tctatttttg atactatcaa tttcaatgcc ttcttgttct agtcgcttga
      841 tggctttcca aattgctgtt cggcttaggg atagtttttc tgcgattttt tctccgctga
      901 tatagtcggt ttctttagat aggatttggt agacagcttg gtaggatttc ataatgttgc
      961 ctttcttact agttggtatt gagagtattc ttttcttgta tgacttggag tctgattaaa
     1021 gtattgttta taagctttcg aaaaatggag tggatctgaa aaacctaccg agtatgcaat
     1081 taccttgatg gactcttggg tattttcgag aagttgtcta gctcggtgca ttcgaacgta
     1141 gagtaggtat tctttgggtg ataaggtatt aaattctttg aatacgcttg ataagtagct
     1201 tctgtgaacg gatagttctt ttgctaaatc ttgaattgta agtgattgag gatagtggct
     1261 atcaattaat cgtttgcatt caagatagag ttggtgggtt gatgaaatat tctttttttt
     1321 ctgattggga gcaatagttc ccagatgaaa catcagttca tgaagttgtc ccatgatatg
     1381 gagttgagct aattcacttg attttgtaat ctgagcgaag cggacaatgt ctgagatgag
     1441 ttttgcagta gtctgggtat gacaagtttc agattggatg agataggatt gatcagaaat
     1501 ttgggaaaga gcaaaataat caggggcttt ccctccagtg attcctaacc agtagtaggc
     1561 ccaaggttct ttactatctg cttgataaaa ggttagttcc tctggtttta atagaaagaa
     1621 atctccttct tttaaatcaa caattttacc cttgtaatga aattttcctt gtcctttagt
     1681 aatgtaatgt aggacgtatg tatcacgaat ggctggacca aaagagtaat taggtgtgca
     1741 ttcctcatat ccataaaagc ttagggcaag gtcgattgtt ccagtctggt attctgaaaa
     1801 aactaacatg tgctacctcc tacctaacat tttaccatat tcttgagaca tttttctatt
     1861 ttggaaagcg atttcaggtg ataaaataga gtcaataaag tgatgaggtg aagtaaatgg
     1921 gagttaggat agagaataat ctattttatg ttgagagtaa aaatctaagt ttgattattg
     1981 aaaatcgaaa tggctactta cttttgaaac atttaggaaa gactattaag aactataaag
     2041 gttccaatag tgtttatgaa cgagaccatg ccttttcagg aaatccaaca gctactaatc
     2101 gaacctttag tttagatact caacgtcaaa tttttggaca acatggctta ggagatttta
     2161 ggaaaccaac catacaggtt cagcataatg taactgaagt aacagacttt cgatttgtag
     2221 aagcaaagat tttaaaaggt cagaatggtc cacagggctt accttctcca catagcatgg
     2281 acgatacaga gactcttgtc ttaatgttag aagattctaa ggctcaactt agtctgactt
     2341 tgtattatac tacttttaat aatgatgcga ctattgctag ctacagtaaa ttagataata
     2401 atagtaatca ggaagttgtc atccataaag atttttcttt tatggctgat tttccagctg
     2461 cagattacga aatagtaact ctgcagggtg cttatgctcg tgaaaagact gttagacgtc
     2521 aacaggtaga acaaggaatc ttttcgatta gttcaaaccg aggtgcttct ggtcatgctc
     2581 aaacaccagc tcttctacta tgcgaacaag gagtcacaga ggatgctggg aatgtgtttg
     2641 ctattcaact aatgtatagt ggcaactttg aagcttttgt tcaaaaaaat caattgaatg
     2701 aagttcgggt ggctattggc attaatccag aaaacttttc ttggaagtta gcccctgagg
     2761 aatactttga aacaccggta gctttagtga ctcattcaga tcagggatta actggtatta
     2821 gtcatgaaag tcagaatttt gtactgaagc acattatgct aagtgaattt tctaaaaaag
     2881 aacgtccaat tctaatcaat aactgggaag ctacttactt tgactttcag agagaaaaac
     2941 tgttagagtt agcagatgaa gctaagaaag ttggcattga actttttgta ttagatgatg
     3001 gttggtttgg caatcgtttt gatgataatc gtgctttagg tgattgggtt gttaatgagg
     3061 aaaaactggg tggaagtcta gaaagtctga tttcagctat ccatgaaaga ggtttgcagt
     3121 ttggactttg gttagaaccc gaaatgattt ctgtagatag tgatttgtat cgtcaacatc
     3181 ctgactgggc tattcaggtt cctggctatg agcatactta ttctcggaat caattagtac
     3241 ttaatcttgc caatcctcag gtagtagaat acttgaaaag tgtcttagat caactcctat
     3301 cttatcatga gattgattac attaaatggg atatgaaccg caatatcact aagctaggga
     3361 atggattaac ttatctagag acacagatgc aatctcatca gtacatgctg gggctttatg
     3421 aactcgtttc ttatctgaca gagaagcaca gccatattct ctttgagtcc tgctctggtg
     3481 gtggtggacg aaatgatctt ggtatgatac gctatttccc acaagtctgg gctagtgata
     3541 atactgatgc cattgcacgt ttaccaattc aatacggttc atcctatttc tatccaacca
     3601 tttctatggg ggctcatgtg tcagcagtac cgaatcatca gatgggacga atgacaccat
     3661 tagaaacacg tggccatgta gcaatgatgg gaaatttggg ctatgagctt gatttgacaa
     3721 atttatcaga tgaagagaaa gctacgattg ctaatcaggt gaacttgtat aaagaattac
     3781 gaccagtagt tcagttagga cagcagtata gactaattaa tcctgatact gcatccaatg
     3841 aagcagctgt acaatttaat tacggaaatc aaacgattgt aacctacgtt cgcgttttat
     3901 ctgttgtaga gaccatggaa acaactttaa aattaaaaga tttggatgaa gagggactat
     3961 ataaattaca ggaaaatggc gaagtttact caggtgcaga actaatgtat gcgggcttaa
     4021 ctgttatctt atcccaagga gattttttga gtagacagta tatttttaga aaactatgat
     4081 atgtactgac cccaaaagtt agatttattc tgtctaactt ttggggtgca gttcaataag
     4141 ggtgtataaa tttataaagg aggctttcta atggaatggt ataaaaaaat cggacttctt
     4201 gcaactacag gtttagcttt gtttgggctc ggcgcttgct ccaactatgg taaatctgcg
     4261 gatggcacag tgaccatcga gtatttcaac cagaaaaaag aaatgaccaa aaccttggaa
     4321 gaaatcactc gtgattttga gaaggaaaac cctaagatca aggtcaaagt cgtcaatgta
     4381 ccaaatgctg gtgaagtatt gaagacacgc gttctcgcag gagatgtgcc tgatgtggtc
     4441 aatatttacc cacagtccat tgaactgcaa gaatgggcaa aagcaggtgt ttttgaagat
     4501 ttgagcaaca aagactacct gaaacgcgtg aaaaatggct acgctgaaaa atatgctgta
     4561 aacgaaaaag tttacaacgt tccttttaca gctaatgctt atggaattta ctacaacaaa
     4621 gataaattcg aagaactggg cttgaaggtt cctgaaacct gggatgaatt tgaacagtta
     4681 gtcaaagata tcgttgctaa aggacaaaca ccatttggaa ttgcaggtgc agatgcttgg
     4741 acactcaatg gttacaatca attagccttt gcgacagcaa caggtggagg aaaagaagca
     4801 aatcaatacc ttcgttattc tcaaccaaat gccattaaat tgtcggatcc gattatgaaa
     4861 gatgatatca aggtcatgga catccttcgc atcaatggct ctaagcaaaa gaactgggaa
     4921 ggtgctggct ataccgatgt tatcggagcc ttcgcacgtg gggatgtcct catgacacca
     4981 aatgggtctt gggcgatcac agcgattaat gaacaaaaac cgaactttaa gattgggacc
     5041 ttcatgattc caggaaaaga aaaaggacaa agcttaaccg ttggtgcggg agacttggca
     5101 tggtctatct cagccaccac caaacatcca aaagaagcca atgcctttgt ggaatatatg
     5161 acccgtccag aagtcatgca aaaatactac gatgtggacg gatctccaac agcgatcgaa
     5221 ggggtcaaac aagcaggaga agattcaccg cttgctggta tgaccgaata tgcctttacg
     5281 gatcgtcact tggtctggtt gcaacaatac tggaccagtg aagcagactt ccataccttg
     5341 accatgaact atgtcttgac cggtgataaa caaggcatgg tcaatgattt gaatgccttc
     5401 tttaacccga tgaaagcgga tgtggattag gagtagagag actatgaaaa aagtattaca
     5461 aaaatattgg gcatgggctt ttgtggtcat ccccctcttg ttacaagcaa ttttcttcta
     5521 tgtgccgatg tttcaaggag ccttttacag ttttaccaac tggacaggat tgacttacaa
     5581 ctacaaattt gtcggcttaa acaactttaa gctcctcttc atggatccaa aattcatgaa
     5641 tgcgattggc tttaccgcaa tcattacgat tgccatggtg gttggtgaga ttgcactcgg
     5701 gatcttcatt gcgcgtgtct tgaactctaa aatcaaaggc caaaccttct tccgtgcttg
     5761 gttcttcttc ccagctgttt tatctggttt gacagtggct ttgatcttca agcaagtctt
     5821 caactacggt cttccagcga ttgggaatgc ccttcatatt gaatttctcc aaaccagtct
     5881 tttagggact aggtggggag caatctttgc ggctgtcttt gtccttcttt ggcaaggggt
     5941 ggctatgccc atcatcatct tcctagctgg tttgcaatct attccaactg agattacaga
     6001 ggcagcaagg attgatggtg cgactagcaa gcaagttttc tggaacattg aattgcctta
     6061 cttgctacca agtgtctcta tagtctttat cctagcccta aaaggtgggc tgactgcctt
     6121 tgaccaagtc tttgccatga ccggtggtgg tccaaacaat gccacaacct cacttgggct
     6181 cttggtttat aactatgcct ttaaaaacaa ccaattcggt tatgccaatg ccattgccgt
     6241 aatcttgttc ttcttaattg tagtgatttc gatcatccaa ttgagagtat ctaagaaatt
     6301 tgaaatttaa gaggagaagc atgatgaaac aagatgaaag aaaagccctg attggcaaat
     6361 acattctatt gattctagga tcggttctga ttttagtgcc gctccttgct accctcttta
     6421 gttccttcaa actcactaag gatattgtag ataatttctt tggctttcca accaacttca
     6481 catgggacaa ctttagccgt ctcttagctg atgggattgg aggctattat tggaactctg
     6541 tcgtcatcac tgtcttgtct ttacttgcag taatgatctt tatccctatg gcagcctact
     6601 ccatcgctcg caatatgagt aaaagaaaag cctttaccat catgtatacc ctcttaatcc
     6661 tcggaatctt cgtacctttc caagtcatca tgattccgat tacggttatg atgagtaaac
     6721 tcggtttggc taataccttt ggtttgatct tgctctactt gacctatgcg attccacaga
     6781 ccctctttct ctatgttggc tatatcaaaa tctcgattcc agaaagtctg gatgaagcag
     6841 cagagatcga tggggctaat caatttacaa cctatttccg catcatcttc ccaatgatga
     6901 aaccgatgca tgcgacaacc atgatcatca atgccctttg gttctggaat gacttcatgt
     6961 tgccactcct tgtcttgaac cgggattcca aaatgtggac tctgcctttg ttccaataca
     7021 actacgcagg ccaatatttc aacgactacg gaccaagctt tgcctcttac gtggtcggca
     7081 ttatcagtat caccattgtc tatctcttct tccaacgcca tatcatttca ggaatgagca
     7141 acggggcagt gaagtaatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag
     7201 gttgctcaaa acactgtttt gaggttgtgg atgaaactga cgaagtcagt aaccatacct
     7261 acggtaaggt gaagctgacg tggtttgaag agattttcga atagtataag aagagctcat
     7321 ggaatctata gtctttgaca cagaggaaga gttcagcttg ttgataattc tatgtagaaa
     7381 acaagaaagt agtactttgt ttatcacagt ctatattagt acgagtttga tgataaatag
     7441 agatttaaaa tgaattttat ttatgaagcc agatcatatg agacttggct tcaattagaa
     7501 aaaggagagt aatgatgcca attcaaaata aaaccatgtt gattacctat tctgatagcc
     7561 ttggaaataa tcttaaagac ttatatgata atttggaaga gcattttgga gatgctattg
     7621 gaggagttca ccttttacca tttttcccat caacaggtga tcgtggattt gcgccagttg
     7681 actacgacga agtggactca gcttttggtg attgggagga tgttaagcgt ttaggtgaga
     7741 gatattatct tatgtttgat tttatgatta atcatatttc tcgtcaatcc aagtattata
     7801 aggactatca agaaaaacat gaagccagtg aatttaaaga tctcttttta aactgggata
     7861 agttttggcc agaaaaccgt ccgacacagt ctgatgtaga tttaatttac aagcgtaagg
     7921 atcgtgcacc aaagcaagag attgtgtttg aagatggttc agtggaacat ttgtggaata
     7981 cctttggtga ggagcagatt gatcttgatg tgaccaaaga agtaactatg gaatttatcc
     8041 gtaagaccat tcagcacttg gcaagtaatg ggtgtgattt gattcgtcta gatgcctttg
     8101 cttatgcagt gaagaaattg gatactaatg atttctttgt ggaaccagat atttgggatt
     8161 tattggacaa agttcgagat atcgctgctg agtatgggac agagctttta cctgagattc
     8221 atgaacacta ttcgattcag tttaaaatag cagaccatga ttactatgtt tatgattttg
     8281 ctcttccaat ggtgacactt tatactcttt acagttccag aacagagcgt ttggctaagt
     8341 ggttaaagat gagcccgatg aagcaattta cgacgctaga tacccatgat gggattggag
     8401 tagtagatgt caaggatatc ctgaccgatg aggagattga ctatgcttca aatgaactct
     8461 ataaggttgg agccaatgtc aaacgtaagt actctagtgc cgagtataac aacttagata
     8521 tctaccaaat caattcaacc tactattcag cgcttggaga tgatgatgtc aagtattttc
     8581 tcgctcgtct aattcaagct tttgccccag gtattcctca ggtttactat gtgggtctat
     8641 tagcaggcaa gaatgacttg aaattattag aagaaactaa agaaggtcga aatattaatc
     8701 gtcattacta tagcaacgag gaaatagcaa aagaagtgca acgatctgtt gtgaaggccc
     8761 ttctcaatct attttctttc cgtaaccgtt cagaagcctt tgatctagaa gggactattg
     8821 agatagagac accaacagtt cacagcattg taatcaaacg tcaaaataaa gataagtccg
     8881 taacagcagt agcagaaatt gatttgcaaa atcagactta tcgggtaatt gagaatggag
     8941 ttgaagtaac attttgaagc cttgatatgg tggataaaat agggttttta ttttggaaaa
     9001 cgtttccttt gttttcaaat tgctaaaaaa gtggtacaat ataggatagc ttactattat
     9061 ctgaatcagc tgatttggag agaaaggatt cattttgaaa tcaataggct ttattgaaaa
     9121 gctgaagggg ttgtctagta aagagctgat tttattggga attatcctaa gtatcttttt
     9181 acccttttat ctttttgtag ttgtactctg tttatatatt atcagtttga tttttacagg
     9241 agacatgaaa agtattcttc agaaaatggg ggagcatccg atgctgcttc tttttcttag
     9301 ctatagtact gttatatcca ttcttgcaca aaattggatg ggtcttgtgg cttcagtagg
     9361 aatgtttcta tttactattt tctttttgca ctatcagtcg attttatccc ataaattctt
     9421 tcgattgatt ttgcagttcg tcttgtttgg tagtgtcttg tcagctgctt ttgccagttt
     9481 agaacatttc caaattgtga agaaatttaa ctatgctttt ctttcaccca atatgcaggt
     9541 gtggcatcag aaccgggcag aagtgacttt ctttaatcct aattattatg gaattatttg
     9601 ttgtttctgt attatgattg ctttctatct gtttacaacg accaagttga attggttgaa
     9661 agtattctgt gtgattgcag gctttgttaa tctctttggt ttgaacttta ctcaaaatcg
     9721 aactgccttt cctgctatta tcgctggagc aattatctat ctctttacga ctattaaaaa
     9781 ctggaaggcc ttttggctta gtattggggt cttcgcgatt ggtttgagtt tcctcttttc
     9841 tagtgatttg ggagttcgaa tgggtacttt agactcttct atggaagaac gcatttctat
     9901 ctgggatgct gggatggcct tgtttaagca aaatcctttt tggggtgaag ggccattgac
     9961 ctatatgcac tcttatcctc ggatacatgc tccttatcat gaacatgccc acagtcttta
    10021 tattgatacg attctgagtt acggaattgt gggtaccatt ttattagttt tgtcttctgt
    10081 tgctcctgtt cgcttgatga tggatatgag tcaggagtcg gggaaacgtc cgattatcgg
    10141 cctttatcta tctttcctta cagtggttgc tgtgcacgga atttttgact tggctctctt
    10201 ctggattcag tcaggcttta ttttcttgct agttatgtgc agtattccat tggagcatcg
    10261 aatgttggta tcggacatga cggattaagt tttataagtt tgaaatcttc taccttgggt
    10321 aggagatttt tttatacgat gggcatgtcg tgcatctgag gtgtaagtcc tctgtaggga
    10381 tccactaccg gtgaatacaa tagcgctccc caagcctgac tatcgtgagg tagcggatta
    10441 aaatggtctg gggatagatc gttttaagtc tgacgcagga aataagaatt gtcagagaaa
    10501 gcaatagtga aatcgtaggc tatgaataga aacgtgatag cgaggagcat atagtggata
    10561 aggtagcgag aaattttaaa gtataaaaga tagtcttaat ctatatatgt aaatcacaaa
    10621 agtaattgaa ttcggaataa ggttggtgtg aaaagacata gtaaactgaa tcttgaatac
    10681 tagaatgata cttctacaat attgttataa tttaatttga attccaaaat cacattgttc
    10741 cgtccgtatt tcattttact gtatgtcttc ctaaactcca aagtttctgc gtcaaatttc
    10801 ctattttcac tgtagtcttt gacacttcga acgacaattt gaatcatata ttcaaaaaaa
    10861 aatgaaaaat ttgtgccaac aagcctaaat attctgaaaa tttgtttgat ttttaatgga
    10921 taatgtgata taatggttgt caatctataa ttcacataaa cagaggagta gaaaatgaaa
    10981 aaaaatcgtg tatttgctac agcaggtctt gttttattag cagcaggtgt acttgcagca
    11041 tgcagttctt caaaatcatc tgattcatca gcccctaaag cttatggcta tgtttataca
    11101 gcagacccag aaaccttgga ctacctgatt tcaagtaaaa atagtacaac agtagtgact
    11161 tcaaatggga ttgatggttt attcactaac gataattacg gtaatcttgc tcctgcagtt
    11221 gcagaggatt gggaagtctc taaggatggt ttgacctaca cttataagat tcgtaaaggg
    11281 gttaaatggt ttacctctga tggagaagaa tatgcagagg tgacggctaa agatttcgtg
    11341 aacggtttaa aacacgcagc agataaaaaa tcagaagcta tgtatttagc tgaaaattcg
    11401 gttaaaggct tggcagatta tctatcagga acttcaacag atttttcaac agttggtgtc
    11461 aaggcggttg atgattatac gttacaatac actttgaacc agcctgaacc gttctggaac
    11521 tctaagttga cctattctat tttctggcct ctgaatgaag aattcgaaac atcaaaagga
    11581 agcgattttg ctaaaccaac agatccgaca tccttgcttt ataatggtcc attcttgttg
    11641 aaagggttga ctgcaaaatc ttctgtagag tttgtaaaaa atgagcaata ttgggataaa
    11701 gaaaatgtcc acctagatac tatcaatcta gcttactatg atggatcaga tcaggagtcg
    11761 ctagagcgta acttcactag tggagcttat agttatgccc gtctttaccc taccagctcc
    11821 aactattcta aggttgcaga agaatacaag gacaatatct attacacaca atcaggctct
    11881 gggattgctg gtctgggtgt gaatattgat cgccaaagtt acaactatac ttctaaaact
    11941 acagattcag agaaagtagc tactaagaag gcattgctta acaaagattt ccgtcaagcc
    12001 ttgaattttg ctcttgatcg ctcagcttac tcagctcaaa tcaatggtaa agatggagca
    12061 gctttagcag ttcgtaattt atttgtaaaa ccagactttg tttcagctgg tgagaagacc
    12121 tttggtgatt tagtcgctgc tcaacttcct gcttatggtg atgagtggaa aggtgtgaat
    12181 ttagctgatg ggcaggatgg tttattcaat gctgacaagg ccaaggcaga gtttgcgaaa
    12241 gctaagaaag ctttagaagc agacggcgtt cagtttccta ttcatctgga cgttccagta
    12301 gaccaagcat caaaaaacta catatctcgt attcagtcct ttaaacaatc tgtagaaaca
    12361 gttcttggtg ttgaaaatgt cgttgttgat attcaacaaa tgacaagtga tgaattcctt
    12421 aatattactt actatgctgc caatgcttca tctgaggatt gggatgtatc aggaggagtt
    12481 tcatgggggc cagactatca agacccatct acttacctgg atattttaaa aacaactagc
    12541 agtgaaacta caaaaacata tttaggattt gataatccaa atagcccttc agtagttcaa
    12601 gttggtttga aagaatacga taaattagtt gatgaagctg ccaaagagac aagcgacttg
    12661 aatgtccgtt atgaaaaata tgcagcggct caagcatggt tgacagatag ttcactcttt
    12721 attcctgcta tggcttcttc tggtgcagca ccagtgcttt cacgaattgt tccatttact
    12781 ggagcttctg cgcaaacagg ctctaagggg tcagatgttt acttcaaata tttgaaatta
    12841 caagataaag tggtgactaa ggaagagtat gaaaaagctc gtgaaaaatg gttgaaagaa
    12901 aaagctgaat caaatgagaa agctcaaaaa gaattggcaa gtcatgtgaa gtaaataatt
    12961 tctaatagaa ctttctctcc atgaggagaa ggttcttttg ggatttttaa aggaaataat
    13021 atgaaaaaat atatttttat gcgtgttttg cggtcattgg tttcgatttt cttagtaacg
    13081 actttgacct acacgattat ctataccttg gttcctcgaa aattgatttt caagcaggat
    13141 cctaactata ataaaattgc gacaacggct gataaacgtg ataactatga aaatactgtg
    13201 tttgagcgta tgggctacat tgagtattac gatactaaag agttgcaaga aaaggcaagt
    13261 agcatggatt cttctgtaac agtagaagca aatgcgacca ataaagctat ttatgaaaag
    13321 tacatcaatc aattaggtca tggttggact ttgggagaat ttactgaaag tggtcaattc
    13381 tatgctactc gtgaaattcc aatttttgaa cgtgtttttc acttctatgc taacttgatt
    13441 gacattgacc atacaaataa aatccaagac cctgaaaatc cagacttgaa acgctacctt
    13501 cgttttgaaa atgatccagc tatcggatgg tcattggtcg gttcaggaac taaacataaa
    13561 tatctcttgt actttaacag tcagttccca tttgttcatc aaaactttgt gaacttgaat
    13621 ttaggtgact cttacccaac ctatgctaat acaccagttc ttcaggttat tactcaaggt
    13681 caaggacaaa ccaaaactgc ccaagttcag ttcccaacag gtaagaaaac gtcttctgta
    13741 aatatttact caagaaccta caagtcacct agtcaggctg actctcgtga agtagctagc
    13801 tatgggaaag atgatcctta tacagcgact gaaagtaatt accaatatcc atctatgatt
    13861 gtcagctctg ctattactgg tttgattggt ttggttcttg cctatgctct tgccgtgcca
    13921 cttggttcag ccatggctcg tttcaagaac acttggattg atagcctctc aacagggact
    13981 ttgaccttct tgcttgctct tccaacgatt gccttggttt acatcgttcg attgattgga
    14041 tcatctattg cccttccaga ttcattccct atcttgggag ctggagattg gcgttcttac
    14101 gttttaccag cagtcatcct tggtttattg ggtgctcctg ttacagccat ttggattcgt
    14161 cgttacatga ttgacttgca atctcaagac tttgttcgtt tcgctcgtgc aaaaggtttg
    14221 tctgaaaaag aaatttcaaa caaacacatc tttaaaaatg ccatggttcc gctggtttca
    14281 ggaattcctg gtgccattat tggggttatc ggtggtgcaa cccttactga aacagtcttc
    14341 gccttcccag gtatgggtaa aatgttgatt gactctgtaa aagcatctaa taactctatg
    14401 gtcgttggtc ttgtcttcat ctttacatgt atttctatct tctcacgtct tttgggagat
    14461 atttggatga ctattattga cccacgtatt aaattgactg agaaaggagg caaataatgt
    14521 ctacaatcga taaagaaaaa tttcagtttg taaaacgtga cgattttgcc tctgaaacta
    14581 ttgatgcgcc agcatattct tactggaaat cagtgtttaa acaatttatg aagaaaaaat
    14641 caactgtagt catgttggga atcttggtag ccatcatttt gataagtttc atctacccaa
    14701 tgttttctaa gtttgatttc aatgatgtca gcaaggtaaa cgactttagt gttcgttata
    14761 tcaagccaaa tgcggagcat tggttcggta ctgacagtaa cggtaaatcg ctctttgacg
    14821 gtgtctggtt cggagctcgt aactccatcc tcatttctgt gattgcgaca gtgattaact
    14881 tggttatcgg tgtttttgtc ggtggtattt ggggtatttc aaaatcagtt gaccgtgtca
    14941 tgatggaagt ttacaacgtc atctcaaaca tcccacctct tttgattgtt attgtcttga
    15001 cttactcaat cggagctgga ttctggaatc tgatttttgc catgagcgta acaacatgga
    15061 ttggtattgc cttcatgatt cgtgtgcaaa tcttgcgcta tcgtgacttg gaatacaact
    15121 tggcgtcacg tactttggga acaccaacct tgaagattgt tgccaaaaat atcatgcctc
    15181 aattggtatc tgttattgtg acaaccatga ctcaaatgct tccaagcttt atctcatacg
    15241 aagccttctt gtctttcttc ggtcttggat taccgattac agtgccaagt ttgggtcgtt
    15301 tgatttcgga ttattcacaa aacgtaacaa ccaatgctta cttgttctgg attccattga
    15361 caacccttgt cttggtatcc ttgtcccttt tcgtagttgg tcaaaactta gcggatgcta
    15421 gtgatccacg tacacataga taggagtaga aatgacaaaa gaaaaaaatg taattttgac
    15481 tgctcgcgat attgtcgtgg aatttgacgt tcgtgacaaa gtattgacag ccattcgcgg
    15541 cgtttccctt gaactagtcg aaggagaagt attagccttg gtaggtgagt caggatcagg
    15601 taaatctgtt ttgacaaaga ccttcacagg tatgctcgaa gaaaatggtc gtattgccca
    15661 aggtagtatt gactaccgtg gtcaggactt gacagcttta tcttctcaca aggattggga
    15721 acaaattcgt ggtgctaaga ttgcgactat cttccaggac ccaatgacta gtttggaccc
    15781 cattaaaaca attggtagtc agattacaga agttattgta aaacaccaag gaaaaacagc
    15841 taaagaagcg aaagaattgg ccattgacta catgaataag gttggcattc cagacgcaga
    15901 tagacgtttt aatgaatacc cattccaata ttctggagga atgcgtcaac gtatcgttat
    15961 tgcgattgcc cttgcctgcc gacctgatgt cttgatctgt gatgagccaa caactgcctt
    16021 ggatgtaact attcaagctc agattattga tttgctaaaa tctttacaaa acgagtatca
    16081 tttcacaaca atctttatta cccacgacct tggtgtggtg gcaagtattg cggataaggt
    16141 agcggttatg tatgcaggag aaatcgttga gtatggaacg gttgaggaag tcttctatga
    16201 ccctcgccat ccatatacat ggagtctctt gtctagcttg cctcagcttg ctgatgataa
    16261 aggggatctt tactcaatcc caggaacacc tccgtcactt tatactgacc tgaaagggga
    16321 tgcttttgcc ttgcgttctg actacgcaat gcagattgac ttcgaacaaa aagctcctca
    16381 attctcagta tcagagacac attgggctaa aacttggctt cttcatgagg atgctccaaa
    16441 agtagaaaaa ccagctgtga ttgcaaatct ccatgataag attcgtgaaa aaatgggatt
    16501 tgcccatctg gctgactagg aggaaggaaa tgtctgaaaa attagtagaa atcaaagatt
    16561 tagaaatttc cttcggtgaa ggaagtaaga agtttgtcgc ggttaaaaat gctaacttct
    16621 ttatcaacaa gggagaaact ttctcgcttg taggtgagtc cggtagtggg aaaacaacta
    16681 ttggtcgtgc tatcatcggt ctaaatgata caagtaatgg agatatcatt tttgatggtc
    16741 aaaagattaa tggtaagaaa tcgcgtgaac aagctgcgga attgattcgt cgaatccaga
    16801 tgattttcca agaccctgcc gcaagtttga atgaacgtgc gactgttgat tatattattt
    16861 ctgaaggtct ttacaatcac cgtttattta aggatgaaga agaacgtaaa gagaaagttc
    16921 aaaatattat ccgtgaagta ggtcttcttg ctgagcactt gactcgttac cctcatgaat
    16981 tctcaggcgg tcaacgtcaa cgtatcggta ttgcccgtgc cttggtcatg caaccagact
    17041 ttgttattgc agatgagcca atttcagcct tggacgtttc tgtacgtgcc caagtcttga
    17101 acttgctcaa aaaattccaa aaagagctcg gcctgaccta tctcttcatc gcccatgact
    17161 tgtcggttgt tcgctttatt tcagatcgta tcgcagttat ttacaagggt gttattgtag
    17221 aggttgcaga aacagaagaa ttgtttaaca atccaattca cccatatact caagccttgc
    17281 tttcagcggt accaatccca gatccaatct tggaacgtaa gaaggtcttg aaggtttacg
    17341 acccaagtca acacgactat gagactgata agccgtctat ggtagaaatc cgtccaggtc
    17401 actatgtttg ggcgaaccaa accgaattgg cacgttatca aaaaggacta aactagtaat
    17461 ggttttataa tttccatgtc aatttttatg gaaattaggc tctttgtcaa ctgtagtggg
    17521 ttgaagtcag ctaagctcga gaaaggacaa atttcgtcct ttcttttttg atgttcaaag
    17581 cgataaaaat ccgttttttg aagttttcaa agtttcgaaa accaaaagca ttgcgcttga
    17641 taagtttgat gagattattg gtcgcttcca gtttggcatt agaatagtgt agttgaaggg
    17701 cgttgataat cttttcttta tctttgagga aggttttaaa gacagtctga aaaataggat
    17761 gaacctgctt aagattgtcc tcgataagtt cgaaaaattt ctctggttcc ttattctgga
    17821 agtgaaaaag caagagctga tagagctgat agtggtgttt caagtcttcc gaataactca
    17881 aaagcttgtt taaaatctct ttattggtta agtgcatacg aaaagtagga cgataaaatt
    17941 gcttatcact cagtttacgg ctatcctgtt gtatgagctt ccagtagtgc ttgatagcct
    18001 tgtgttcatg ggattttcga tgaaactgat tcatgatttg gacacgcaca cgactcatag
    18061 cacggctaag atgttgtaca atgtgaaagc gatcaagaac gattttagca ttcgggagtg
    18121 aaacagtct
//
LOCUS       DAVLYR010000018        17321 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLYR010000018 DAVLYR010000000
VERSION     DAVLYR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.018"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(413..2140)
                     /gene="ezrA"
                     /locus_tag="VYO63_000587"
     CDS             complement(413..2140)
                     /gene="ezrA"
                     /locus_tag="VYO63_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1671559.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPPQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2222..4168)
                     /gene="gyrB"
                     /locus_tag="VYO63_000588"
     CDS             complement(2222..4168)
                     /gene="gyrB"
                     /locus_tag="VYO63_000588"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1671560.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVYVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4183..4746)
                     /locus_tag="VYO63_000589"
     CDS             complement(4183..4746)
                     /locus_tag="VYO63_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1671561.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4831..5385)
                     /locus_tag="VYO63_000590"
     CDS             complement(4831..5385)
                     /locus_tag="VYO63_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1671562.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5396..6619)
                     /locus_tag="VYO63_000591"
     CDS             complement(5396..6619)
                     /locus_tag="VYO63_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1671563.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6656..9106)
                     /locus_tag="VYO63_000592"
     CDS             complement(6656..9106)
                     /locus_tag="VYO63_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1671564.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVVLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9306..10235)
                     /locus_tag="VYO63_000593"
     CDS             complement(9306..10235)
                     /locus_tag="VYO63_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1671565.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10375..10662
                     /locus_tag="VYO63_000594"
     CDS             10375..10662
                     /locus_tag="VYO63_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1671566.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10696..12030)
                     /gene="ciaH"
                     /locus_tag="VYO63_000595"
     CDS             complement(10696..12030)
                     /gene="ciaH"
                     /locus_tag="VYO63_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1671567.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12020..12694)
                     /gene="ciaR"
                     /locus_tag="VYO63_000596"
     CDS             complement(12020..12694)
                     /gene="ciaR"
                     /locus_tag="VYO63_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1671568.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12803..15349)
                     /locus_tag="VYO63_000597"
     CDS             complement(12803..15349)
                     /locus_tag="VYO63_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1671569.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15472..15912)
                     /locus_tag="VYO63_000598"
     CDS             complement(15472..15912)
                     /locus_tag="VYO63_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1671570.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQAHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15887..16756)
                     /locus_tag="VYO63_000599"
     CDS             complement(15887..16756)
                     /locus_tag="VYO63_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1671571.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPSVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16798..>17321)
                     /locus_tag="VYO63_000600"
     CDS             complement(16798..>17321)
                     /locus_tag="VYO63_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671572.1"
                     /translation="TNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLNNLVTHLEEGYN
                     IVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNELIAGISSGLVYR
                     KAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASKKVAEFLGYELS
                     EPYEAFELEEILI"
BASE COUNT         5235 a         3719 c         3132 g         5235 t
ORIGIN      
        1 tttaccgtgg actaaagttg tacaagaaaa atgcaaataa gaaatctcca gattaggaac
       61 tatccgtgag ttctctagtc tggagatttt tcaatagact tcgttattgg acggttacca
      121 agttactcaa agtacagatt tgaagctgca gataagactg acgaaatcac tttaatacta
      181 aatgaaaatc aaaaagcaaa ctaagaagct agcctcaggc tgtacttgag tacggtaagg
      241 cgacgctgac gtgatttgaa gagattttcg aagagtataa aacactgttt tgaggtggtg
      301 gataaatcgg ctgggtaaaa actcaatctc aggagaaaat gaagtaaatc tccccacaac
      361 tatagaaaaa gatttgattc tgctcctcac acaataaaat ctttttcttt tattaaaaac
      421 gaatcgtttc acgtgttttc tcatatgagg taacaaagcg attggttaca ccaggctctg
      481 ccacttccaa tgcttgagaa atcttatcaa atgaagcgtg ataatcaaat tctttttcaa
      541 aaatatctaa agcttcgtta aatgcttctt gaatgcgttc atcaaatgag cgatagcggt
      601 tagaatattg caagagttgc tctgtcaaag ttgcatattg tacaatatta taagtttccg
      661 tttctaaagc ttccatatca ttcgttgcaa tttcaagaac tcgggtaaca gattcaatgt
      721 taatcatttt ttgttctaac tcaaccatta aatcctcggt attattgctt gccgtaaaga
      781 ataacttcaa gaaagtttgt ggaatacctg gcagattgcg tttttccatg tatcgcttga
      841 tagtatggag acgattgaca taaacattgg ccttttgacg tgcattaata tcatctttct
      901 caatttgtgt caggcgctca ctaactgaaa tttgctcatc ttcaatatct tttagttgag
      961 tttgtaaatc ctcaagattt tcttcaagaa ctgaataagc ttggggtggt tcttcttgat
     1021 ttgaagttac ctcaacaata gctgcctcaa aactctctaa ttctgtctga atacgacgaa
     1081 catggcttgc agctgtctca ggaagtaaat aggtcttgtt caaacgtgca atatcttctc
     1141 ccaataaagt attattctct ttcatatgtt gaagataagt tggaagagtt gcaagtagat
     1201 tttccactac tttctgagca gcaatttctc gagtaaaaat atcatacaag gcattgattt
     1261 cctcttgtgc ctgtccattc tcatattcgg cattatccaa ttccaactga cgaatattct
     1321 cttggttttt cttgaatgct tcataaagca agtggaaacg cgcttcaata tccgtttcaa
     1381 caaaatgata attagcatca attagtttac gataaccggc ttccaaatcc tgtaattgat
     1441 ctggcaattc tgtagaaagc gtcgtaacca aggctggaac acgatccaca atatgactta
     1501 aggccaaaat gtgattttct gtattatcca aaatcactgc ggcttccaca gggtcacccg
     1561 atgaattcaa ggttacaaat tgtgaaaatt cagattggat attttctaat tgtttttcaa
     1621 tttcatccaa ggcttgacca tactgttctg aattttcagc aactctatgc tgaagttcct
     1681 caaataaatc caaagcatga agaacacgac cactattttt agattcttgc ttctctaagt
     1741 ctgccaaagc attgcgaatt gccgcaatat cttcttcaat caaagtaatt tgactctcaa
     1801 tttggtcaat ttgatgactg gccttgagaa aacgaaatga atggttataa ccttctgctt
     1861 caaagagatt attttcaata tcggcaaaag agttgagaga taaatcgacc catttttgat
     1921 tccattcacg gaaagccact tgactttgtc caatcaagtg catatttttt acagcttcta
     1981 cttcatcatt tactggaaga ttgtatagtt cttcttttct ttcttctagt gcctctaatc
     2041 tcccctcgtt tcgcttacgt agaaagattg ccactacata agccagaact aaaatgactg
     2101 caattgcaac cattaaataa attagttgtc cattagacat atcaaactcc ttttttactt
     2161 gaaacaatcg taacgattat atcatatttt ttagaccaag ggaactactt ctccctattt
     2221 tttagacatc aagtgtacta tagacagcat tttcttcgat aaactcacga cgaggctcta
     2281 ctcgatcccc catcaacata tcaaagattt tatctgcttc tgcagcatca tctacagaaa
     2341 ctctagccat caagcgatgt tcgggatcca tggttgtttc ccacagctga tgatcgtcca
     2401 tttcacctag ccccttataa cgctgaatag tcggtttggt acgaccttca ctataacggg
     2461 ctaaagcttc ttggagtttg atttcttgat ctgcacccgg ctggatatat tctttaatct
     2521 cgcttccaac cttgacacca tagattggtg gttgggcaat ataaacataa ccagcttcta
     2581 ggattggttt catataacga taaatcaagg ttaaaagaag ggtacgaata tgggctccat
     2641 cgacatcggc atcggtcatc aaaacgagtt tttggtaacg ggctttcgaa acatcaaatt
     2701 ctgcgccaaa tcctgttccc atggctgtga aaagactacg aatttcttcg ttagctagaa
     2761 tcttatccat acttgctttt tcaacgttca aaatcttacc gcgaattgga aggatagcct
     2821 gaaactcacg gttacgacca gatttggctg atccaccagc tgagtctcct tcgacgatga
     2881 agagttctgt ttcagcaggg ttattagaag aacagtctgc tagtttccct ggaaggttgg
     2941 aaatttccaa accagatttt ttacgtgtga cttcacgcgc acgcttggca gccacacgag
     3001 ccttggcagc taaaatccct ttttccacga tacgtttggc aatctgtgga ttttccatga
     3061 ggaaatcgga gaaggcttca ctgaagaggc gattggtaat cttgaccact tcgctatttc
     3121 ccaatttggt cttggtttgt ccttcaaact gtggatttgg gtgtttaact gagataactg
     3181 cagttaatcc ttcgcgaaca tcttcccctg ttaaattatc ttcattgtct ttcagtaact
     3241 tattcttacg agcataatca ttgataacac gagtcagtgc cgtacggaaa ccttgttcat
     3301 gcgttccacc ttcatgggta tgaatattat tggcgaaact catgacattt tcatggtaac
     3361 ctgttgtgta ctgcatggct acttcaactg taatatcatc catctcaccg tctgtataga
     3421 ttggtgtatc aaagattaca tccttgttct cgttgatata ttcaacgtaa ctagcaatcc
     3481 caccttcata atgataatgc ttggtttgtt ccaaaccttg gcgcttatca gtgatggaaa
     3541 tttgaagacc gcgatttaga aaggccaact cttgaatccg tttatttaat ttatcaaaat
     3601 caaagattgt tgtttcagtg aaaatttctg ggtccggtgt gaagtgaacc gttgttccag
     3661 ttctatctgt atccccaacc acctcaaggt ctgcaacaac atgaccacga cggtattctt
     3721 ggtaatgaat cttaccgttt ttatggacat aaacgtctaa ttgagtggaa agggcattaa
     3781 ctactgacga ccccaccccg tgaagaccac ctgaaacctt gtatccacca ccgccgaact
     3841 ttcctccagc atgaaggact gtaaagacgg tctcaacagc aggacggcct gttttttcct
     3901 gaatatcgac tgggatacca cgcccatcat ccacaacagt aatcgaatca tctggctcaa
     3961 taaaaacttg aatatggctg gcaaatcctg ccaaggcctc gtcaattgag ttatcaacaa
     4021 tttcccagac tagatggtga agaccttctt ttgaggttga tccaatgtac atccctggac
     4081 gcatacgaac agcctctaag ccctctaaaa cttgaatttg actggcatca taatcctgtg
     4141 cctgcagatt tttgatttct tctgtcatct tattcctttt tcttatatgt ctaatacttg
     4201 tcttaaattc acgatactgg taaacaagtg ggtatcaagt cctgcagctt gacctgcttc
     4261 gatatcaatc ggccgatcac caatgacaag accagagcta atctgatact tttctcttaa
     4321 ataaagcatg gattcgggat ttggctttct cttaaagcct gagctagaag tcaccacttc
     4381 tgtaaaataa gctgctatag aggttttttc taaaatttcc aaaacctgat catttcgatg
     4441 agagaccaaa aaatgacggc caccttgatt tgaaatgact tccaataggt cagaaactcc
     4501 ttcaaataaa atcgggtgtt caagctctct ggcttcattt tccttgtact tttctaaaaa
     4561 attctctaaa ttgggagcga atgtctcaat cgcaaaagga gtagaaacct ttaaagcttg
     4621 atagacactg tcatggtctt gtgtgatacc atacagtgcc aatgtttcaa caaatgcagc
     4681 tgttgaagtt tcataattat ccagtaaagt tccacctaaa tcccagatgt aatcgtgata
     4741 tttcatgccc tttattatag cattttttta tgaaaaaagc gatgatttcc ctgagttttc
     4801 cacataaaaa acgagagaat aactcccgtt ttactgattt ttaccaaaga catcttgata
     4861 gagcattcct gttcgtaaac tctctgcgtc ccctcctagt tgctccacca actcgtaggc
     4921 aaaggcaagg gctgttgaag gaccccgact ggttgtcaac tgaccatcta ccactactgt
     4981 ttccttgacg tagtgaccat caaggatttg ctcttgaacg ccgtcataac aagtgtatcg
     5041 cttatttttc aatatctctg cttgattgag ggcaattggt gccgcacaaa tggctgctag
     5101 tttcttccct tcttgctcga agctttgcaa ttcttgaatc aaggtctgat tatcacgtaa
     5161 atgtgcagaa ccaggcatac ctccaggaag aacaatcata tcatagtctg ataaatctcc
     5221 atcaaagaca tgatctgctc ttacttggat tgcatgcgaa cccgttactt gctcttcaaa
     5281 accaaccata tcacatgtga tattggctcg acgcaagaca tctacaactg tcaaggcttc
     5341 aatttcttca aagccctgag ctaacataac tgctactttt accatgattt tctccttatt
     5401 taatttgttt taatacaacc tttttccgtt tgaatgggac ttttccgctc ttttcttcag
     5461 ctaaattagt ctggtaactc atcgataaaa gcaaaccaac accaatcaaa ttactgataa
     5521 tagctgatcc cccttgcgaa atgaaaggca agggaatccc cgtcaaagga agtagtccag
     5581 tcacagcacc gatattctca aagatgtgga agagcaacat cataatcaaa cctgtggaaa
     5641 tataagtgta gaactggtta tttgatttaa gagtaatctt caacatacgg taaatcaaca
     5701 tgagatagag ggcaataacc aggacagagc caataaagcc aaaatcttct gcaataaccg
     5761 taaaaatcat atctgactct cgaactggga taagcagatt cgaagcatta aatccctgac
     5821 caaataagcc accactccca atggcaatct gcccttgagc ctgctggtaa gtcgttgttt
     5881 gggcaaactc aaagggattg agccaagcca aaatccgatt aatttggtag gtcggcattc
     5941 caatctggtg aagaaaagct cgtccgtcct tgctaataaa gatagctaag aaaccagcaa
     6001 ctcctgttac agcagtcaca aatactggga taataatttt ccaagaaacc cctgataata
     6061 aaacgattcc tgagaaaatg gctacaaaaa ccaaagccgt ccccaagtca ctttgaagtg
     6121 ctaaaagaac taggactgga atggtaaaga gaatcatcca gaaaattaac aaaaagtcca
     6181 gcggaaccgt gcgtctccat tccttatgtt tctttgtaaa ttggacaatg acacgagcca
     6241 acatgaggat ataggatatc ttcataaatt ctgacggttg gaatagggta attccattta
     6301 ttgataccca gtttttggca cccgttgatg caactaagct tggattataa aatacaatcg
     6361 gcaagatcat aagtcccaag cctaaaatat atagaaaggg ggtcaccttc caaagaaatt
     6421 ctgtattaaa gagcatgacc acaaaaccaa tcacaagccc caaggcaatc caggcgacct
     6481 gctgccctaa aatgggcaga atattattgg gataatcatg actaacggct atatagatag
     6541 ccaccacacc gatgaccagt agaaaaaata ctggcaagag caaactgtaa tcgactcttg
     6601 agtcgagaga acgtttcata tatactaacc ttatactttc atacaatact atttatcaaa
     6661 gttcattaaa aaatctatca atagcctcgt caacttcgga tcgagagatg gttttaacag
     6721 tcgcttcttc tgctagagat gctactattt gtttgccgta tcgttttccg acgattctcc
     6781 tatccaaaat aagagttaag gaacgttggt attcacgtct catacttctt cccaaagcct
     6841 gttttaaacg aataatggcc attggcaatt gataatcata aaaggcattt ttcccttctt
     6901 gattcagttc ttgattaatc tttttcgtca agggttcttg aggattttgg aaaggaagcc
     6961 tcggtacaac ttgaatcaca aaaggatggc ttgaaaaatc aactccctcc cagaaacttg
     7021 ctgcaccaag caagatttgt tgttcacctt tttcaaagcg tttctttagc tgatgaacat
     7081 ccccattttt atactgggcc aagtggctaa ctgtaagtaa atccgatact gctagaagca
     7141 tgtctttagc ggtaaagaga accaaaatcg gttgctggaa agcttgaatt tccactagta
     7201 aagcagctac ctctctggca tagacttcta aggaggtttc tgttaccaga gggaaatctt
     7261 tgaccataac cacttcttgt tcctgttttc cccgagattc aatcttgaca aatttggctt
     7321 caggatagcc taaaaggtct gccaaagaaa ccctctgact aatctcaaga gtagccgata
     7381 ctcccaagac ttggcaactc tcaggaagta aagaggacag aagagtacgg cctgattttg
     7441 tagaagaaat ctgaattttc ttttgactcg tttcagttac ttcaagccag taatcacctt
     7501 cagctgtaaa atagcgaacc agctcatcaa agccttctac ttctaattct gaaaaatact
     7561 gatggagatt gtccagagaa tctaagatat ttttcctaga tttaccagac tgaaattgtt
     7621 ctatcaagta gagacactca aagcgaatac tttctagtat ccgttgttga accctgtttt
     7681 cttctcctac taaagcctta tcaattaaat cgataataga ttgtatatcg taggtctctt
     7741 gaagcagatt ttctagagct aacaaaatct tttggacttc atcaataatc agtaaacggt
     7801 cactgacaaa ttcaggatta tcttcaagtc tggtcacgag ataggcatga ttagtcacta
     7861 aaagcttgca agtctctgcc ctttcttgac tacgtttcca aaaatcttcc gtcacaaata
     7921 agctctggga tgataaattc ccatcatgac gaaggtctgc tagaaaatgt tggtaacggt
     7981 agagttgccc gatttcatcc aaatctcctg tctctgtctc agtaagccag accaagactt
     8041 gcattttaaa gcgtctaaat aagcgatttt catcattttc ctgcaaggaa tgataaaagg
     8101 catccaactt cagataattt tgtggtccct ttaagctatg aatatctgta tggaacactt
     8161 ccttgagacg tttaccttct tcttccatga tttgattttg aagaatcttt gtcggaacac
     8221 taagaacaat ttgtcgctct ttggattgag ataaagcggg taagagatag ccataggttt
     8281 tcccaatccc tgtcggcgct tgaatcagag agacaggttc atctttcaat agcaagacaa
     8341 cctctttagc aaaactttct tgttcctccc tcacttcaag gttcaacaga gaaatatttt
     8401 tagaaaagtc ttgagatagt tttcgtagct ccagagaagc tgccgttttc ttaaaataca
     8461 gaccttgaac ttggaccaag tctggagaac tcaggataga ttggttgcga taagtttcct
     8521 caataaccag gtaggactca tataggagag cgtcagccat ttccagcaag cgttccaaga
     8581 gacctttagg aagctgggtc atcttttccc gtaaaaaaag aagtaattct gctgtagctt
     8641 gggcatctga aagggctgtg tgtgcgtgtt taagaggaat tcctaattct cgacacaaaa
     8701 tcggcaagct atatttttcc agttcaggga aaaagacctg ggccaattcg accgtatcaa
     8761 cacgagggtt tcttagctca tagccttcaa aaaataaatt ttccgccaag agattagcat
     8821 caaactgaac attatgggct acaaaaatcc catcctccac caagtcaaat atttttctgg
     8881 caacttgcga aaaatcaggt gcttgcgcca gacgttggtc tgtcaatcct gtcagttctt
     8941 tgatatgagc atccaagggt tcatgtggat tgacatccgt cgtatagtga tcgacgattt
     9001 ctccgtcctc aatcacgaca attcccactt ggataatttt agccttacta cctgtgctag
     9061 ttgcctctaa atccaccaca acatagcgat taccaatcgt tttcattata aaaaattata
     9121 ccaaaaaaag ggctatttgg cacctgcaaa cataggataa ttttcaaact caagaacact
     9181 acttttctta aaaatcccat cgaatagagc attctagcct gctgattttt gtaaaatagt
     9241 gaagaaatac gaaaaattga gagaagaaat gttttcatct tccttctctc aacgaatcat
     9301 gtattttatt taaccatgtc aggatcgtag tcataaaatc ctgtatcaag gaaacggttt
     9361 ttagggtgaa gtttacgaac ttcttcatcc agcaagaagg cttgatcatg gctaaagttg
     9421 ccctcttgaa tcaagttacg cgcttggata aagagttttg caatctcaac aaagttctga
     9481 ccaggagtgt agagcccttc tagtttccag cgagtaaagc catgctccac caattctgtt
     9541 aatttgatca tcaaatcaag gtcattgttg gcaaagatat gggtcccatg attatcttca
     9601 aaaatggaat agtggctctc tggatcactt ggctcagcca agaagaggtc acgtttatgc
     9661 gtcttttcat catcgatatg tgtaaagtta tagtagtttt gcaagagtgg acgtttagaa
     9721 tgatggatga cgctagcacc gtaaaccaaa acttcagcag gaatttccaa aatctctggc
     9781 attttgaaaa gttcagctga tggaatttca cgcgccaaaa cagcctcaga tgcaccagcc
     9841 ttttgtcccc agaagttaat ctgacgactg ctagttacca tggttgaagc atcgtagatg
     9901 gtcttaaatg aataaccatc gcggttaact acgtaaaaga cgcctgcatc cccaatcgta
     9961 atatagtctg tcttgatttc ttccaagaag tctaagaaag gcttgatacg gtccatcata
    10021 tcttggtgca tgagagcatt gaccgcaacg agcaattcct taccagcatc atgaaccaac
    10081 ttagcgattt cacgtaattg gtcataacta aaggtcgttg gcagacgaag gccaaaatct
    10141 ttctcaccga catagatacg gtctacgcca gcttcgagta gttgttcaac ttgttcaata
    10201 ctttcagcag ttgctgtaat gacaatcttt tccataagaa aaattatacc acatttctcc
    10261 aaaatcagcc atgctttgaa aatgaaaagg gactttattc tttttgtaac ctttctgtaa
    10321 taattctctg ccgaaaaaat gataaaatag gtatgtactg ttaaggagag aatcatgccc
    10381 gtaagaaaat tacaatccta tgaggtagac tatcaagaag aattaaacca gcagcttcct
    10441 cattaccaag cttatacacc tgaagcgcaa tctgatgcca atctcaagga aatcctattt
    10501 tttattaata tcgctgtttt ttgtatctgt attgctatct ttagttttat ctttttagca
    10561 ttaaaattat caactgctct tgcctttgcc gcagcaatcg gattcagctt acttgtttta
    10621 aaagttcaac ggtctattat caaacgaaaa cgtagaagat aaatccaaaa tatcgcccca
    10681 attggagcga tatttttatt ttttcttttt agatggtgtc tgaatggcaa tcttcacttc
    10741 aaagattgtt cccttgggtt tattatcttt gacagtaaca gttcctttta gagcatctac
    10801 aatttgcttg gctagggata atcctaaacc aaaaccacct ttttgccggg ttctagcctt
    10861 gtctactcga taaaaacggt caaaaatttt ctttttatct tctgtcgaaa taccgattcc
    10921 attatcagaa acaagtaaat aaagattgcg atcggtcgcc gagataagaa aatcaatttc
    10981 accatcctcc tcagtatact tgacggcatt atcgaaaaga atggtcatca gttgtttcag
    11041 aagaagctga tctgtgacaa ttgttcgatg gatacgattt tcaaaacgga agacacgatt
    11101 attttccgaa gcaatcatct catagtttgt gaaagttgta ttaaaaaagc tagttggaac
    11161 ttctgcaagc tccggcttaa tcccatcatc tctccgagct aagttcagca agcttgtcgt
    11221 taaaaaacgc atatttcgga cttcttccaa actcgatgca atgctttcgc tcacatccat
    11281 aatggtagct tctggcttac gaaaaagggt ctctaagcga ttttgcaaaa ctgcgagtgg
    11341 agttcgtaac tcatgactgg cattttccac aaaagactgt tgcttctgca tactctcaag
    11401 caagggccta acactgaccc tagctagata gagactggca agtaaagaca aaatccagaa
    11461 actagccatc acgaccacaa tcaattgctc atgcttttga ctagcctgct ccaactgact
    11521 ggtattaatc aagacagcag catacttgat attggttgaa accgaactaa tattggtctc
    11581 catcaaaatc acacgataga tttcttcctg cccatagcta ttaaaaacct gaatctggta
    11641 gatatgtcct agttctttct tttctaactt aatcttatcc aagcccaaaa atcgatttcc
    11701 agaaagaagt tgagtaaagt ctttatcaaa gagaatgact tccgtattgg aactgacatt
    11761 aggttttatt tctactttac tagcgtccgc cctagcattt tctaaatctt taatctcttc
    11821 tgttgcccta tttatagcca gctgaataac tgcttgagga ttttcactca gtccatgaag
    11881 cttatcgtcc accgaagtat aaagactcga atgcatgact tgtaaaataa tcagagtcat
    11941 tgtagaaaaa atcagggtga agacaccgaa gttgcggata aaataactaa agtcatccgc
    12001 ataccatgtt tttttaagtt tactgaacat cttttaaaag atacccaaca ctacgcaagg
    12061 tttgcaaatt ctctgcaaaa gtggttccct ttaatttctt acggactttt gaaacataga
    12121 cttcgacaac cgaaatcgtt gtatcactat caaatcccca tagacggtca aaaatctgcg
    12181 tcttaggcaa aatcacattt tgattttgaa ggaaataaac tagtaaatcg aactctttcc
    12241 ccagcaattc gacaggagta tcttcaactt taacggtatt ggttgataaa ttaaccacga
    12301 tattcccata agtcaaggtg ttttcattaa acttccctga acgtttgaga agggcctgaa
    12361 tccgcatttt aagttcttct aggtagaaag gtttggtcag ataatcatcc gctcccagtt
    12421 caaatccatg tcccttgtca tccaaacttt ccttggcagt cataatcaga actggtgtcg
    12481 taattccctt ttcacgcaat tcttttaaga cttggaaacc atttttttct ggcaacatca
    12541 aatccagcaa aatcaagtca tagacaccac tctcagcttc gtagagacct tcttctccat
    12601 caaatacctg cataacatcc gcaaaatcgt ctaaaaagtc aaatactgaa tttgacagac
    12661 ctaggtcatc ctcaaccaat aagattttta tcatgagaaa ctcctcctta ttaaaactat
    12721 tataccaaat ttgccttaaa aaaaactcaa ctctctgcat tttacatgag atagctgagt
    12781 tttcttttta ttttaggctt atttatgcat ttccgtattg aagaacaact gcttcgactg
    12841 cagctttttc acggctaatc aagtcaacac gtgctgcaat ttccttgatt cccataccga
    12901 tgttacggct aagagcaagg tcagaaagtt gcggctcaaa gaactccttg tattccgcca
    12961 agcgttgctg agtcttaaat acatgagcag gaaggataac aaagctatca aagctcatat
    13021 ctcctccaag ggctgcctta atccaagccc agttttcacg cgcccaagac caagctgttt
    13081 tctgagttgc ttgatgagct aggaattggt aataccaagc agacaagtcc tgtggtttga
    13141 ccacaaattt gtccttccaa gaagtaatca agttttggat attatccgca tctgtactgt
    13201 atgcaagagc tgctgccaac tggcgtttaa agacagcatc tgttgcgtga gtataagtat
    13261 caagataaag tgctaacaag tctttagtct catgatgttt catctcatta atcagaactt
    13321 gtgaacgaat agctgctggg agtcctgcaa gattctcctt gtgtgttgcg aagatttggc
    13381 tagcgacttg actagcttct gcatcatttg agcgaatcat catcgaaaca gccagctgac
    13441 gaaccaattc atcctcatct gattctccgt ctttagcttc aaaaccaaga cggtcatagt
    13501 tatgacgagc caatttagca accagtcctt tgaaggctgt ttcagcatcc gttccttcat
    13561 caataaagcg ctcaagggct gaaatcactt gagaaacagc tgaaaccacc agataagact
    13621 cttccttagc aagtttatca agaactggaa gcaagtctgc ataagaaatg tgccctgcct
    13681 cagccaacaa acgacgttct tgaacaattt gcagtttgct tgtgttatca agtgtctcta
    13741 gctcagcaag aacagcttct aacaagtctc cttgatagtc ggtaatatag tgggcagtat
    13801 tttcagtgtt gagacgaaga gctccttcat tttcagcaag aagagctgcg tagccaggga
    13861 tttcgatact ttcagtttcg agtgtatcag gcaagccttt ccagttgcta ttgaggggca
    13921 ccacccagag acggttcttg tcttcgttct caccgatgaa gaattgtttt tgtgaaatct
    13981 tcaagacatc attttcaact ttaacagtaa gaactgggta accaggctgt tccaaccaag
    14041 aatccatgaa ggctgcgaca tcacgtcctg acgcttgacc aagggcatcc caaaggtcac
    14101 taccaatggt gttgctgtat tggtgttttt caaagtaggc gtgcaaacct ttagcaaaat
    14161 cagcatctcc tagccaacgg cgaagcatgt gcatgagacg gcttcctttg gcatagacga
    14221 tagcgccgtc aaagagtgta ttgatttcat ctggatgttt aacttcgacg tggacagact
    14281 gaacgccatc agtagcgtca cgttcaagag caagaggtac tccacctgtt tggaaatctt
    14341 caaagatatt ccagcttggt tcgatggtat ccacacagac gtattccatc atattagcaa
    14401 aactttcatt gagccaaagg tcatcccacc atttcatagt cacgaggttc ccaaaccatt
    14461 ggtgagccaa ttcatgggcc acaacaaggg caacttgttg acggctagca aatgtagagt
    14521 tctcatcgac aaccaagtaa acttcacggt aggtcacaag accccagttt tccatagcac
    14581 cagctgagaa gtcaggaagg gcgatgtgga gagattgagg aattgggtac ttaactccat
    14641 agtaatcttc gtaaaactcg atagagcgaa cagcgatatc cagtgagaaa tcaagatttg
    14701 aaagtggatg tgctttggtt gagtagacac ctaccagggt accattttta gttttagcgg
    14761 tcaccccttg caaatcacca gcaacaaagg ccaacaagta agaagacatg cgaggtgttg
    14821 tctcaaactt ccagatacct gtttccttac ggttttcaac atcgatttct ggcatgtttg
    14881 acaaggccaa ttcaccttct gcttggtcaa agcgaagaga gagatcaaaa gttgctttgg
    14941 cttcaggctc atccacacat gggaaagctt cgcgcgcaaa atggctctcg aactgagtag
    15001 acaagacctc cttcttgact ccatcaactg tataataaga agggtaaatc cctgtcatgt
    15061 tgtctgtaat tttaccagaa aaggcaagaa ccaattcaac ttgaccagcc tcagccaatt
    15121 cgatatgaag ggcttcattg tcatggtcaa ctgtaaatgg acgagcttga cctgcaactt
    15181 ctacagaggt gatttccaag tctttttggt ggagggagat gcggtcactc tgtgcttgac
    15241 cagtgatggt cactttccca gaaaaagtct tggtctcacg actcaaatct aaaaataaat
    15301 cataatgttc aggaacaaat tgcttaataa aatgttcaac tgcttgcata gttttctcct
    15361 attctaagtt taagagctgc tgctcttctt caaacaaact tattatatca tattttgctt
    15421 gagaaaaaag gattggacag cgatttccaa aactttcttc cttctaatag tctacagtgg
    15481 gtagaccttg cgaaattctt ctaaaacaac cttgctttca ggtgtaaaag tcagccctac
    15541 ttcacccaaa cagtctgtga aaaagtcctc atgagcctgg cgccaatagg caagtgattt
    15601 gtctccctca ccttccttaa aggcatgttg cgcagagact tgattgaaga gttcaacaga
    15661 aacctttgta atttcgacaa tgcagacaga ctgattttga ctatctaaaa tgacatcgaa
    15721 ggtccctact tggggaagtg gttcgtcttc tagtacatag aggtcatagg ctgatgctgt
    15781 tgctgtcttt tctcctttaa acaccaaatc cgctaaaagg tctggttcaa ctccaaaagc
    15841 ccaagcatcg atttcatctc cgatcaaagg attgatttgc ttgtatttat tccacatttc
    15901 ttgcggtatc atgggtactc ctttgtaatt ttttactttc ttcttttatg tgtttaagat
    15961 gatctggatg gtcaatctct aaatcaaaaa tctctggaat agaactgtag tggataatgc
    16021 acttgatacc caactgattc attttttgta tgaaagaagt attcagatag cctgctacag
    16081 caaaattaat cttgttcttt cttgctttat cctgcatatc tcttagcata tctaacatta
    16141 ttggactttc catatcatgc cattgactgt ttctcatagt cgcaaaaaca aaggaagtca
    16201 aatcattcat tccaactaca atctttgaaa tgcccgtttc cagtatacta gataagtcaa
    16261 aatacgctga cggtaattca atcatcgttc cgactttccc agtaaagcca tactgacgca
    16321 atactgtaat agcttgtttt aattggtcgg catcattgac agaaggaaag ataagagata
    16381 gattggggtt ggtttgataa acttctgtaa cgacatgtgc ttcagcctga aattcatcca
    16441 aacacgccag taaacgtctg gttcctctat agccaaacaa gggatgccct tcgtcaaaaa
    16501 actctctagt ccccactaaa caattggctt ctgtattcgt taattcagaa aaacgatacc
    16561 aaacttcttc atctgagtac agggagcaga tagtctctag ataatctttt acaaattgct
    16621 gacaacttgc taatatgatg ttttgattaa gctctctcaa caaatattcc ccacgaatca
    16681 tgccgacgtg gtgaaatagt tgaggataaa ttttttcaag aattttttcg ccactaaggg
    16741 caagttgatt tctcatagcc attcacctcg tcttaaaaga tacaatatca tatataatta
    16801 tatcaaaatt tcctctagtt caaaagcttc ataaggttca gataactcat atcctaaaaa
    16861 ttctgctact tttttggaag cctcattatg agcatcccaa agtggaaaca tatctctatt
    16921 taaactctct agaatcattg cagcaccaag ctttttagca aatccatttc cttgttcgtt
    16981 tggtctagtt gcaacttcca cttcaactgc tttacggtaa actaaccctg aggaaatccc
    17041 agcaatcagt tcattatttt taagaatcac aaagccaaat ccacctttta aaacaaaatc
    17101 ctgataggac tcaaaatccc cctgtaaatc ctgtgaccat tcttccgtag aaaagcagtt
    17161 ataaatatga ttatcaatag gcacaatatt gtaaccttcc tctaaatgag taactagatt
    17221 atttaaaaat tcaacttgaa aatttgcctt atctttaaaa gaatagcgag taaaagaatt
    17281 tagccccact tttcgatgca gaaaatcttg ccaattagta t
//
LOCUS       DAVLYR010000019        17283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLYR010000019 DAVLYR010000000
VERSION     DAVLYR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.019"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..656
                     /locus_tag="VYO63_000601"
                     /pseudo
     CDS             1..656
                     /locus_tag="VYO63_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(720..1802)
                     /locus_tag="VYO63_000602"
     CDS             complement(720..1802)
                     /locus_tag="VYO63_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1671573.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(1799..2776)
                     /locus_tag="VYO63_000603"
     CDS             complement(1799..2776)
                     /locus_tag="VYO63_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1671574.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            2991..3368
                     /locus_tag="VYO63_000604"
     CDS             2991..3368
                     /locus_tag="VYO63_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1671575.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            3603..4994
                     /locus_tag="VYO63_000605"
     CDS             3603..4994
                     /locus_tag="VYO63_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1671576.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            5108..5950
                     /locus_tag="VYO63_000606"
     CDS             5108..5950
                     /locus_tag="VYO63_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1671577.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            6223..6984
                     /locus_tag="VYO63_000607"
     CDS             6223..6984
                     /locus_tag="VYO63_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1671578.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            7177..8094
                     /gene="glyQ"
                     /locus_tag="VYO63_000608"
     CDS             7177..8094
                     /gene="glyQ"
                     /locus_tag="VYO63_000608"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1671579.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            8306..10342
                     /locus_tag="VYO63_000609"
     CDS             8306..10342
                     /locus_tag="VYO63_000609"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1671580.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            10384..10641
                     /locus_tag="VYO63_000610"
     CDS             10384..10641
                     /locus_tag="VYO63_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1671581.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(10864..12108)
                     /locus_tag="VYO63_000611"
     CDS             complement(10864..12108)
                     /locus_tag="VYO63_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1671582.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(12127..12732)
                     /locus_tag="VYO63_000612"
     CDS             complement(12127..12732)
                     /locus_tag="VYO63_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1671583.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            complement(12792..13715)
                     /locus_tag="VYO63_000613"
     CDS             complement(12792..13715)
                     /locus_tag="VYO63_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1671584.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14009..15388
                     /locus_tag="VYO63_000614"
     CDS             14009..15388
                     /locus_tag="VYO63_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1671585.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            15540..16415
                     /gene="pdxS"
                     /locus_tag="VYO63_000615"
     CDS             15540..16415
                     /gene="pdxS"
                     /locus_tag="VYO63_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1671586.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            16416..17000
                     /gene="pdxT"
                     /locus_tag="VYO63_000616"
     CDS             16416..17000
                     /gene="pdxT"
                     /locus_tag="VYO63_000616"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1671587.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEDVEILATVDNQIVA
                     AQEKNMLVTSFHPELTDDAHLHQYFIEMCKEKRQ"
BASE COUNT         5251 a         3155 c         3809 g         5068 t
ORIGIN      
        1 ccatggttgg cgaaatatta tgccacgtcc agaacatcct aggaaagcag acgctcaaac
       61 cattgtcgcg tctaaaaata aaatctcaat tcaagaagac aagaaagcgc tttaaaacca
      121 gtagaccttt tcataaggtt cgcttgatgt accaagatga ggctggtttc ggtagaatca
      181 gtaaactggg atcttgttgg tctccaatag gagtaggtcc acatgtccat agtcactata
      241 tacgagaatt tcgctattgt tatggagctg ttgatgcccc atacaggcga atcatttttc
      301 ttaatagctg gtggatgtaa tactgagtgg atgaacgtct ttttagaaga gctttcacaa
      361 gcttatccag atgattattt tttactcgtt atggacaatg ctatatggca taaatcaagt
      421 accttaaaga ttccgactaa tattgatttt acctttattc ctccatacac accagagatg
      481 aacccattga acaagtgtgg aaagagattc gtaaacgtgg atttaagaat aaagcctttc
      541 gaactttgga agatgtcatg aatcaactcc aagatgtcat acaaggactg gagaaggagg
      601 tgataaagtc catcgttaat cggagatgga atagaatgct ttttgaaagc agatgagtat
      661 aaaattgaat tgcttataaa aaagctccat acactggatg tgtatagagc aatggggctt
      721 tatttgatat agagttcttg gttttttagg acaatttctc ggatacttgc aaacttttta
      781 agttttttga tttcttctgg atgagtgacg agagtaataa catatccttc cttgcccata
      841 cggccagtac ggccagcacg gtgtgtgtag gtttcgctat ctctaggaat atcaaagttt
      901 acgacacatt ctaggctatc gatatcaatt ccacgagcca aaaggtcagt tgcaagaagc
      961 agggttagtt ggttatcttt aaacttttct aagatgattt ttctaaattt aacattaaca
     1021 tcactagcga gggaaacagc caatatatca cgatactgta gtttttcctc ggcattccca
     1081 aggtctgaca ggctattgaa gaagactaga ccacggaaat cctctacatg agccagtttt
     1141 cgcagcatat ccactcgatg acgttggtct acctgcatgt agaaatgctg gatattgtcc
     1201 aatttttgat cagagagatc aatagtgcgt gtattcggca caatcttttc ttggtcaaac
     1261 ttggtcgtgg cactcatgta gaccagttgg tggtcacgag gtgcgtagtg agtgattttt
     1321 tctacaaagt gaatctgaga atcatctagt aattggtcaa attcatccag gatgatggtt
     1381 tccacattca tcatcttgat ttttttaagt ttaatgagtt caaagatacg gccaggagtt
     1441 ccaatcagaa tttctggccc ctttttaaga cgttcaattt gtcgtttctg acttgaacct
     1501 gaaaggaaga gttgagcagt caatccgata gcttctgccc acgttttaca tacatcaaaa
     1561 atctgtccag caagttccgt atttggtgct agaatcaaga gttgttgggc ttttttcttt
     1621 tgtagtctga gaagacttgg taggagatac gctagggtct taccagttcc ggtttggctc
     1681 actcctagga ggttttctcc agcaagaagg ggctcaaata gttgagtttg aatgggggtg
     1741 aattcttgga aaccgagttg gtcactcagt tcttgccatt cagtcggtag tttggttttc
     1801 atttttctgc ctcaaatcta atgccagcag tctggcgcat ggtatatagt agctcatgaa
     1861 cagagcctgc atcatacagc caagtttggt agagattcag atctggttgc tggatcatgt
     1921 gtgcaaatgc agcgacttcc tcagtcatcg tatgaggagc ctgttggata ggaagctgga
     1981 cttgatttcc ttggtggtcg gtaaaaatag ctgagcgaat atgctcaatc gtgttgagag
     2041 tcaaggttcc atctgttgta taaatctcgc aaggaagatt ggaagtgatg ttttttccag
     2101 ccttgatgtg aacttgatag tctgggtaga agaggatacc atctccattt aggtcaatgc
     2161 tattgtcaag ctgttgagca tggtaagtcg cgtcattagc tttttcaaaa agacgaacag
     2221 cagcatagag gggataaatc cccaaatcca tgagggctcc accagcaaaa cggtctgaaa
     2281 agacatttgg tgtttgtcca gccaacaagt caggcatctt ggaagagtat ttggcatagt
     2341 tgaaatctgc tcctaacact tgcttatctg ctaaaaagtt tttgatagta gtaaaggctt
     2401 tctcgtggta attacgagct gcttcaaaga taaaacagtt atttttttca gctgtttgaa
     2461 tcaaatcaaa ccattcttgt ggttgagtga cagctggctt ttcgagaata acatgtttac
     2521 cagcagacaa ggcagctttt gcctgagcaa aatgtaagga gtttggactg gcgatataga
     2581 ctaaatcaaa agaagatttg aagaagactt ctaattgatc gaatagttgg atattctgat
     2641 agcgagaagc aaaggttgct gcagtttcta gttttctaga atagattgcg accagttggt
     2701 attctccact ggtatgggct gcttctatga aatgatggct gatagcgcca gttccgatga
     2761 cacctaattt tagcataaat actccttttc cgattttaaa tccttctttc attataacat
     2821 agatagacgg gactatccaa cagagaggag aaaatttcaa ataagctatt agctttcttt
     2881 tccgaataaa tagatagaag catagaatct agcaaaccta gatttaaaaa tgtgctataa
     2941 tagaaggagg aaaaggagga ttctcagaca tccaggtatc agcccaacta atgatttgtc
     3001 aatttatccg cgatatgctg gacttgccag caaaaaatgt gacgattttg gagggaagta
     3061 acattcacgt cttgccttcc atgccctact cagcgcaaga tttctatact agtatagacg
     3121 tcttggcgga gttagataat ggaatccaag ttatcatcga aattcaggtt catcatcaga
     3181 attttttcat caatcgccta tgggcttatc tgtgcagtca ggttaatcaa aacctagaaa
     3241 aaattcgcca acgtgaaggt gatacccacc agagctacaa acaaatcgca ccagtatacg
     3301 ctatcgcaat tgtcgatagt aattacttct cagatgacct agcttttcat agttttatag
     3361 taaaatgaaa tgagaacagg acaaatcgat caggacaatc aaatcgattt ctaacaatgt
     3421 tttagaagta taggtctact attctagctt caatctacta gaaattccat agatataaaa
     3481 ctacataatc tctacagata cggatgttgg agttgatgta agatgctttg gcttgctaga
     3541 ggaattgtgg attgccaaat tgtatcattg aaattattgc tcaaatttgt tatgatataa
     3601 atatgaataa aagtagacta ggacgtggca gacacgggaa aacgagacat gtattattgg
     3661 ctttgattgg tattttagca atttctattt gcctattagg cggatttatt gcttttaaga
     3721 tctaccagca aaaaagtttt gagcaaaaga ttgaatcgct caaaaaagag aaagatgatc
     3781 aattgagtga gggaaatcag aaggagcatt ttcgtcaggg gcaagccgaa gtgattgcct
     3841 attatcctct ccaaggggag aaagtgattt cctctgttag ggagttgata aatcaagatg
     3901 ttaaggacaa gctagaaagt aaggacaatc ttgttttcta ctatacagag caagaagagt
     3961 caggtttaaa gggagtcgtt aatcgtaatg tgaccaaaca aatctatgat ttagttgctt
     4021 ttaaggttga agagactgaa aagaccagtc taggaaaggt tcacttaaca gaagatggcc
     4081 aaccttttac acttgaccaa ctgttttcag atgctagtaa ggctaaggaa cagctgataa
     4141 aagagttgac ctccttcata gaggataaaa aaatagagca agaccagagt gagcagattg
     4201 taaaaaactt ctctgaccaa gacttgtctg catggaattt tgattacaag gatagtcaga
     4261 ttatccttta tccaagtcct gtggttgaaa atttagaaga gatagccttg ccagtatctg
     4321 ctttctttga tgttatccaa tcttcgtact tactcgaaaa agatgcggcc ttgtaccaat
     4381 cttactttga taagaaatat caaaaagttg tcgctctaac ctttgatgat ggtccaaatc
     4441 cagcaacgac cccgcaggta ttagagaccc tagctaaata tgatattaaa gcgactttct
     4501 ttgtgcttgg gaaaaatgtt tctgggaatg aggacttggt gaagaggata aaatctgaag
     4561 gtcatgttgt tggaaaccat agttggagcc atccgattct ctcgcaactc tctcttgatg
     4621 aagctaaaaa gcagattact gatactgagg atgtgctaac taaagtgctg ggttctagtt
     4681 ctaaactcat gcgtccacct tatggtgcta ttacagatga tattcgcaat agcttggatt
     4741 tgagctttat catgtgggat gtggatagtc tggactggaa gagtaaaaat gaagcatcta
     4801 ttttgacaga aattcagcat caagtagcta acggctctat cgttttgatg catgatattc
     4861 acagtccgac agtcaatgcc ttgccaaggg tcattgagta tttgaaaaat caaggttata
     4921 cctttgtgac cataccagag atgctcaata ctcgcctaaa agctcatgag ctgtactata
     4981 gtcgtgatga ataagcaaga aaaaataggt ctgttagata tttgacagac ttatttttta
     5041 cagaagatag tactacttaa aaaatgtttt atgctataat tgatgaataa aatagaagga
     5101 gaagcatatg aatacctatc aattaaataa tggagtagaa attccagtat tgggatttgg
     5161 aacttttaag gctaaggatg gagaagaagc ctatcgtgca gtgttagaag ccttgaaggc
     5221 tggttatcgt catattgata cggcggcgat ttatcagaat gaagaaagtg ttggtcaagc
     5281 aatcaaagat agcggagttc cacgtgaaga aatgttcgta actaccaagc tttggaatag
     5341 tcagcaaacc tatgagcaaa ctcgtcaagc tttggaaaaa tctatagaaa aactgggctt
     5401 ggattatttg gatttgtatt tgattcattg gccgaaccca aaaccgctca gagaaaatga
     5461 cgcatggaaa actcgcaatg cggaagtttg gagagcgatg gaagacctct atcaagaagg
     5521 gaaaatccgt gctatcggcg ttagcaattt tcttccccat catttggatg ccttgcttga
     5581 aactgcaact atcgttcctg cggtcaatca agttcgcttg gcgccaggtg tgtatcaaga
     5641 tcaagtcgta gcttactgtc gtgaaaaggg aattttattg gaagcttggg ggccttttgg
     5701 tcagggagaa ctatttgata gcaagcaagt gcaagaaatc gcagcaaatc acggaaaatc
     5761 gattgctcag atagccttgg cctggagctt ggcagaagga tttttaccac ttccaaaatc
     5821 tgtcacaacc tctcgtattc aagctaatct tgattgcttt ggaattgaac tgagtcatga
     5881 ggagagagaa aacttaaaaa cgattgctgt tcaatcgggt gctccacgag ttgatgatgt
     5941 ggatttctag aaaatcataa aaagaattgt acattattct aatttttgat ataatagtca
     6001 gcaggaaaga aagtcttatg gcgttcttca agcgagcttg ggatagtggg agccaagtag
     6061 ggcaaaataa tgagctggcg ctttctgtag tattttcaaa aacaatgaag taataaatta
     6121 gggtggaacc gcgtttctga cgcccctagg ttaaatcaac ctaggattgt cggacgcggt
     6181 tcttttgctt attcagtcta ttgtgtgaaa gaaaggagag ccgtggacaa cctttatctt
     6241 gtaaaagacg atagtcaact agctacattt cgtgattttg tagtaagaaa tactgaaaag
     6301 ttgaaagatt atcaatcttt tttaaagaat gaacttgcag tctgtgattt accgcaagcc
     6361 gttatttggt cagattttaa tgctgctaca cagattatta gggaaagcgc tgttccagcc
     6421 tatacaaata atagacgaat ggttatggcg cctgatttag ctgtttggaa agagttgtat
     6481 ttgtatcaat tgatggacta cgagtgttct cagcaaactc aagcaataga aagtcactat
     6541 cattctttat ctgaaaattt cctcttacag attgtaggac atgagttagc tcattggtcg
     6601 gagcattttt tagatgattt tgatggttat gactcttata tctggttcga agaggggatg
     6661 gttgaatata ttagtcgcaa gtatttcttg acagaagagg aatttcaagc ggaaaaaatt
     6721 tgtaatcaat ctctcgtaga actttttcag aagaagtatg gttggcattc attgaatgat
     6781 tttggttctt cgacttatga taagaactat gcaagtattt tttatgaata ctggcgcagc
     6841 tttttgacag tagataagtt ggtagaaaat ttaggtagtg tacaagcggt cttagattct
     6901 tatcatttat gggcaaatac agaaaaaact tttcccttgt tagattggtt tgttcagcag
     6961 aaattaattg aaaaagaaat ataaaaacta aaggagtaaa caatgtctaa gaaattgaca
     7021 tttcactgcg tcagtggcag aaacctcctt acagtcggac tgccccacgc tcagcactag
     7081 agtgcctgag ctagacgcag tactaactcg tcttgcctcg tatgatcgac gaggcagact
     7141 catgtcgcaa gtaatttata taaaaaggag taaacaatgt ctaagaaatt gactttccag
     7201 gaaatcatcc ttactttgca acaattttgg aatgaccaag gttgtatgct tatgcaggct
     7261 tatgataatg aaaaaggtgc gggaacaatg agtccttaca ctttccttcg tgctatcgga
     7321 cctgagccat ggaatgcagc ttatgtagag ccatcacgtc gtcctgctga cggtcgttat
     7381 ggggaaaacc ctaaccgtct ctaccaacac caccaattcc aggtggtcat gaagccttct
     7441 ccatcaaata tccaagaact ttaccttgaa tctttggaaa aattgggaat caatcctttg
     7501 gagcacgata ttcgttttgt tgaggacaac tgggaaaacc catcaactgg ttcagctggt
     7561 cttggttggg aagtttggct tgacggaatg gaaatcactc agttcactta tttccaacaa
     7621 gtcggtggat tggcaactgg ccctgtgact gcggaagtta cctatggttt ggagcgcttg
     7681 gcttcttaca ttcaagaagt agactctgtc tatgatatcg agtgggctga tggtgtaaaa
     7741 tacggagaaa tctttatcca gcctgagtat gagcactcaa aatattcatt tgaaatttcg
     7801 gaccaagaaa tgttgcttga aaactttgat aagtttgaaa aagaagctgg tcgtgcatta
     7861 gaagaaggct tggtacaccc tgcctatgac tatgttctca aatgttcaca tacctttaat
     7921 ctgcttgacg cgcgtggtgc cgtatctgta acagagcgtg caggctatat cgctcgtatc
     7981 cgtaacttgg cccgtgtcgt agccaaaacc tttgtcgcag aacgcaaacg cctaggctac
     8041 ccacttttgg atgaagaaac aagagctaaa ctcctagcag aagacgcaga ataaagagag
     8101 tgacaaatta cgaaaatggg cgaacagagt gagccctgag ccagttgccg cagtgatgaa
     8161 ggtatcctta gtgaaactaa ggatactagg caaaattgga gacttttggc tccaatttta
     8221 gcaatgaaac aacgaagttg gttgcttgcg tgccaatcac ataaggcaaa ctggaaaata
     8281 aaaagatact tttcggagaa aaaacatgac aaaaaactta ttagtagaac tcggtcttga
     8341 agaattacca gcctatgttg tcacaccaag tgaaaaacaa ctaggcgaaa aaatggcagc
     8401 cttcctcaag gaaaaccgcc tgtcttttga agccattcaa accttctcaa caccacgtcg
     8461 tttggctgtt cgtgtgactg gtttatcaga caaacagtct gatttgacag aagatttcaa
     8521 gggaccagca aagaaaattg ccttggatag tgatggaaac ttcaccaaag cagctcaagg
     8581 atttgtccgt gggaaaggtt tgactgttga agatatcgaa ttccgtgaaa ttaagggtga
     8641 agaatatgtc tatgtcacta aggaagaagt tggccaatca gttgaagcca ttgttcctgg
     8701 tgttgtagat gtcttgaagt cattgacttt ccctgtcagc atgcactggg ctggaaatag
     8761 ctttgaatac atccgccctg ttcacacttt aactgttctc ttggatgagc aagagtttga
     8821 cttggatttc cttgatatca agggaagtcg tgtgagccgt ggccatcgtt tcttggggaa
     8881 agaaaccaaa attcagtcag cattgagcta tgaagaagac cttcgtaagc agtttgtaat
     8941 cgcagatcca tgtgaacgtg agcaaatgat tgttgaccaa atcaaggaaa ttgaggcaaa
     9001 acatggtgta cgtatcgaaa ttgatgcgga tttgctgaat gaagtcttga atttggttga
     9061 atacccaact gccttcatgg gaagttttga tgctaaatac cttgaagttc cagaagaagt
     9121 cttggtgact tctatgaagg aacaccagcg ttactttgtt gttcgtgatc aagatggaaa
     9181 actcttgcca aacttcattt ctgttcgtaa cggaaacgca gagcgtttga aaaatgtcat
     9241 caaaggaaat gaaaaagtct tagtagcccg cttggaagac ggagaattct tctggcgtga
     9301 agaccaaaaa ttggtgattt cagatcttgt tgaaaaatta aacaatgtca ccttccatga
     9361 gaagattggt tcccttcgtg aacacatgat tcgtacgggt caaatcactg tacttttggc
     9421 agaaaaagct ggtttgtcag tggatgaaac agttgacctt gctcgtgcag cagccattta
     9481 caagtttgac ttgttgacag gtatggttgg tgaatttgac gaactccaag gaattatggg
     9541 tgaaaaatac acccttcttg ctggtgaaac tccagcggtg gcagctgcta ttcgtgaaca
     9601 ctacatgcct acatcagctg aaggagaact tccagagagc aaggtcggcg cagttctagc
     9661 cattgcagac aaattggata cgattttgag tttcttctca gtaggattga ttccatcagg
     9721 ttctaatgac ccttatgccc ttcgtcgtgc aactcaaggt gtggttcgta tcttggatgc
     9781 ctttggttgg cacattgcta tggatgagct gattgatagc ctttatgcat tgaaatttga
     9841 cagtttgact tatgaaaata aagcagaggt tatggacttt atcaaggctc gtgttgataa
     9901 gatgatgggc tctactccaa aagatatcaa ggaagcagtt cttgcaggtt caaactttgt
     9961 tgtggcagat atgttggaag cagcaagtgc tctcgtagaa gtaagcaagg aagaagattt
    10021 taaaccatct gttgaatcac tttctcgtgc ctttaacctg gctgagaagg cagaaggggt
    10081 tgctacagtt gattcagcac tatttgagaa tgaccaagaa aaagctttgg cagaagcagt
    10141 agaaacactc gttttatcag gacctgcaag tcagcaattg aaacaactct ttgcgcttag
    10201 cccagtcatt gatgctttct ttgaaaatac tatggtaatg gctgaagatc aggctgtccg
    10261 tcaaaatcgt ttggcaatct tgtcacaact aaccaagaaa gcagctaagt ttgcttgttt
    10321 taaccaaatt aacactaaat aaaatttgat aaacggactt tatcttatta caaaggagaa
    10381 gaaatggatc cgaaaaaaat tgctcgtatc aatgagcttg ctaaaaagaa aaaaacagaa
    10441 ggcttaacac cagaagaaaa agtggaacaa gccaaactac gtgaggagta catcgaaggt
    10501 tatcgccgcg ctgttcgtca ccacattgaa ggaatcaaaa ttgtggacga agaaggaaac
    10561 gatgttacac cagaaaaact acgccaagta caacgtgaaa aaggattaca tggccgtagt
    10621 cttgatgatc caaattcata ataatactct tcgaaaatca aattcaaacc acgtcagctt
    10681 caccttgccg tacttaagta cagcctgcgg ctagcttcct agtttgctct ttgattttca
    10741 ttgagtatat gtattctttc ttttaacaaa gatagatgaa acgataacaa agagactagc
    10801 agtttgtgtt tgctagtctt ttttcgctaa aaaaggaacc ataatggttc ctaaaaacta
    10861 tcattagtaa cttgcaccgg ctgtagcgtc tgcgtcacca ccgtggcctc cagcatcccc
    10921 tgaatcagaa gcgccagaag tagcatcggc gtctccatga cctccggcag caggagcaaa
    10981 tggtccgcta ccacccacca aacgttgacc agtctctttt aggtaccagt caagccatgg
    11041 ttggaagtta aagacgattt cattgatacc agcgtatgat ccatcaggat agtacattgc
    11101 ttggtagttg tgagtgttga taacacctgc aggagaacct ggaacgatcg tacggacgta
    11161 ttcttggttt ccgttgcgaa gtgttccgat aacccactct acgttcttca tacgtgctgg
    11221 tggaagagaa ccatgaacag tcgacatacg gctacctgat tgaggtggta cacgtttagc
    11281 gaacatagtg tctggatctt ggtgagcgtt gttgtagtag aggaattggt tgttgtcgtc
    11341 agcgtatgtc aattcaaatg gcatagcttt caagaacata tcaatttggt taactgttag
    11401 gataccgtgg tccaatttga cataggtatc accagaaaca gcaccagtga atgctgcaac
    11461 tttttctacc cattctggat cgtcagggtc aacttctgtg atggttgtag cgattggttt
    11521 tccacaatcc aagtcttctg attcgattgg ttttggtttt ttcaatttcg aaacgactcc
    11581 tacgtattta acaaagttat ctaagcaagt ttcaaggaat ttaacagtgc cttcgttggt
    11641 gatatttccg ttgttatcaa aagcttcctt agctttacca agaaggaatt cgttacctgg
    11701 aagcgtgtag gcattaacac ctggagcatc aaggatttta cgaaggtgaa cttgagcacg
    11761 tgatgttcct tggtcatagt atgatgcacc cacaatcata acaggcttgt tttcaaatgg
    11821 atgaacttcg tatgaaagcc attcaagtac agatttgagt gaagctgaga tagtgtggtt
    11881 atgctcagga gtagcaatga taacaccatc tgcacgagta attttgttat ataaataacg
    11941 taattggaaa ctttcatccc atttttcatc ttggttaaac attggaactt cgtcaatttc
    12001 aagaacttct aattcaaatt tgagtttgaa gtagcgacgg ataaattcca agagcttacg
    12061 gttatatgat tgatcgtagt ttgatccaac aagtccaaca aatttcattc tttttggtct
    12121 cctatcttac aaattttccc agtcaaagtc ttcagcatct ttgcgaagta attcttgtgc
    12181 attacgtaat ttttctgtga tttttacaaa gatacggaag tcatcaaaga tggcatccaa
    12241 tttcttgata acatcaaggt caaccaagtc gccacttggg ttaaatgctt gaagagagtg
    12301 tgagagcaag aattcatctg gaagaacatt tgccttgatt tcaggagcat tcaagatttg
    12361 acgaagttgc aattgggcac gagatgaacc aagcgtaccg taagaagcac ctgtaatcat
    12421 gattggtttg ttcaaaagtg ggtaaatacc ataagataac caagcaagag cgctcatcaa
    12481 aacagctgga atagagtgat catactcagg agtaccgata ataacgccat ctgcctcttc
    12541 gattttagca gcaatttcca atatttcagc aggtacttgc ttgtcagctg gtttgttgaa
    12601 gacaggaatg gccttgattt caacaagttc aatttcagct ttgtcagtaa agtgtttttg
    12661 catgtattga agcaattgac ggtttgtaga acgttttgaa tttgttccaa caatagcaat
    12721 aagttttaac atgagatttc ctttctcttt ttacataata caattttaaa attccattga
    12781 aacagttgtc tctatagagt aggaattcct gaagaacagc ttaggtggcc ttctttatcg
    12841 atgaggatga cttcgatgcc ctccaaactt tcgacttgcc agaggataga agcaggtctt
    12901 tctccaaaga gtcgagtcgt ccagatttcg ccatcgactg atttatcaga gatgattgtt
    12961 agactcgcta gttccgtttc aacaggatat cctgtttgac tgtcaaaaat gtgatggtaa
    13021 tcttgtccat cgacggtcag gtgacgttca taaatgcctg aagtcacgac agatttattg
    13081 acaacaggga tggtcattaa atgatttccc ctaggattgg ctgggtcttg aatcccgatt
    13141 tgccatgggt tatcccctct tgcctgattt tttccaatgg tcaggatatt ccctcccaga
    13201 ttgatcaagg cagaagtcac cccctctttc ctaagaaatt gggcaacctt atccgcactg
    13261 tatcctttgg ctaaacaacc tagatcgatc ttcattcctt tctgttttaa aaacacagta
    13321 gaagtagaag aatctaactc gataccatga ggattgatta gaggcagcac cgattcaatt
    13381 tcttgaggct gggcgacctt ggcatctgaa aaaccgatac gccaggtttg aattaaggga
    13441 ccaatgctga tattgaggtg gctagagagc gctaggctat gctctaaccc aagtgaaatc
    13501 agctcaaaca ggtctggatg aaccgtgacg ggggctattc ctgcttgata attgatttcc
    13561 atcaactcag attcttgact attggcgttg aagcggtatt caagttcttt gagcaagtca
    13621 aaggattttt ggagaaagat atcggcttgc tcatccacta atgaaatagt gatagtagtc
    13681 cccattagcc gttcagaatg tgaacgaaga gtcaagctac caactccttt ctcttataga
    13741 aaataagttg taatatcaaa taatcatcta aattgaagcc cttacatttc attttcatgt
    13801 tattataata ccataaagtt agaattttca caaacaaaat ttggaaaaag tcaagaaata
    13861 tgctcataaa attcatcagg cttgaaaaca ggataaatgg ggaattattt ttgataaaaa
    13921 atgctgaaat aatagtaccc cccttgtaaa cgctaacggt aaatggtata ctagtaaggt
    13981 aaatttagaa tgaaggcagg aaatttttat gagtaaaatc gttgtagtcg gtgctaacca
    14041 cgctggtaca gcatgtatca ataccatgtt ggataatttt ggaaatgaga acgaaattgt
    14101 tgtatttgac caaaactcta acatctcttt cctaggatgt ggaatggctc tttggattgg
    14161 tgaacaaatt gacggtgctg aaggcttgtt ctattctgat aaagaaaaat tggaagctaa
    14221 aggtgctaaa gtttacatga actcacctgt tctttcaatc gactatgata acaaagtagt
    14281 tacagcggaa gttgaaggaa aagagcacaa agaatcatac gaaaaattga ttttcgctac
    14341 aggttctaca ccaatcttgc caccaatcga aggtgttgaa attgttaaag gaaaccgcga
    14401 atttaaagca actcttgaaa acgtacaatt cgtgaaattg taccaaaatg ctgaagaagt
    14461 tatcaataaa ctttctgaca agagccaaca cctcgaccgt atcgccgttg ttggtggtgg
    14521 ttacatcggt gttgaacttg ctgaagcctt tgaacgtctt ggaaaagaag ttgtccttgt
    14581 tgatatcgtt gatactgtct tgaacggtta ctatgacaaa gacttcacac aaatgatggc
    14641 gaagaacttg gaagatcaca acatccgctt ggctctaggt caaactgtta aagcaatcga
    14701 aggtgacggt aaagttgaac gcttgattac tgacaaagaa agctttgacg tggatatggt
    14761 tatccttgca gttggtttcc gtccaaacac agcccttgca ggtggtaaga tcgaactctt
    14821 ccgcaacggt gccttccttg tagacaagaa acaagaaaca tctatcccag acgtttacgc
    14881 tgttggtgac tgtgcgactg tttatgacaa tgctcgtaaa gatacaagct atatcgctct
    14941 tgcttcaaat gctgttcgca ctggtatcgt tggtgcctac aatgcttgtg gacatgaatt
    15001 ggaaggaatc ggtgttcaag gttcaaatgg tatctcaatc tacggtcttc acatggtttc
    15061 aactggtttg actcttgaaa aagcgaaagc tgctggttac aacgcaactg aaacaggctt
    15121 taacgatctt caaaaaccag aattcatgaa acatgacaac catgaagtag caattaagat
    15181 tgtctttgac aaagatagcc gtgaaattct tggtgcccaa atggtttccc atgatattgc
    15241 aattagcatg ggaatccaca tgttctcact tgctatccaa gagcatgtga caattgataa
    15301 attggcattg acagacctct tcttcttgcc acacttcaac aaaccataca actacatcac
    15361 aatggctgcc cttacggctg aaaaataaaa atgaatgagc tatctggcct taagttaagg
    15421 tcagatagtt tttagctaat ctgtccccat acaattatag tttttttatc ttgtgcttca
    15481 ttctgttctg acttaaaatg aaaaggtagc taccaataca aatgatgagg ataaaacaaa
    15541 tgactgaaaa tcgttatgaa ctaaataaaa acttggcaca gatgctcaag ggtggtgtta
    15601 ttatggatgt gcagaatcct gaacaggctc gtatcgcaga agctgctggt gcggcagctg
    15661 tgatggcctt ggaacgaatt ccggctgata ttcgtgcagc tggaggagtt tcccgcatga
    15721 gcgacccaaa gatgattaag gaaatccaag aagcggttag tattccagta atggctaagg
    15781 tcagaatcgg gcattttgtt gaagctcaga ttttagaggc tattgaaatt gattatatcg
    15841 acgagagtga agttctatct ccagctgatg accgtttcca tgtggacaag aaagaattcc
    15901 aagttccttt tgtctgtggt gctaaggatt tgggtgaagc cttgcgtcgt atcgctgaag
    15961 gtgcttccat gattcgtacc aaaggagaac cagggacagg ggatatcgtc caagctgttc
    16021 gtcatatgcg tatgatgaat caggaaattc gccgcattca aaacttacgt gaggacgagc
    16081 tttatgttgc tgccaaggat ttgcaagtcc ctgtagaatt ggtccaatat gttcatgaac
    16141 atggaaaatt gccagttgta aatttcgctg ctggaggtgt tgcaacgcca gcagatgctg
    16201 cgttaatgat gcaactaggg gcagaggggg tctttgtcgg ttcaggtatt ttcaagtcag
    16261 gagatcctgt taaacgagcg agtgccattg ttaaggctgt gactaacttc cgtaatcctc
    16321 aaatcctagc tcaaatctct gaagatttag gagaagccat ggttggtatt aatgaaaatg
    16381 aaatccaaat tctcatggct gaacgaggaa aatagatgaa aatcggaata ttggccttgc
    16441 aaggggcctt tgcagaacat gcaaaagtgc tagatcaatt aggtgtcgag agtgtagaac
    16501 tcagaaatct agatgatttt cagcaagatc agagtgactt gtcgggtttg attttgcctg
    16561 gtggtgagtc tacaaccatg ggcaagctct tacgtgacca gaacatgcta cttcccatcc
    16621 gagaagccat tctatctggc ttaccagtgt ttgggacctg tgcgggctta attttgctgg
    16681 ctaaggaaat cacttctcag aaagagagtc atctaggaac tatggatatg gtggtcgagc
    16741 gtaatgctta tgggcgccaa ttaggaagtt tctacacgga agcagaatgt aagggagttg
    16801 gcaagattcc aatgaccttt atccgtggtc caattatcag cagtgttgga gaagatgtag
    16861 aaattctagc aacagttgat aatcaaatcg ttgcagccca agaaaaaaat atgttggtaa
    16921 cttcttttca tccagaattg acagatgatg ctcacttaca ccagtacttt atcgaaatgt
    16981 gtaaagaaaa aagacagtaa aaaagctcaa aaaatcactt cctgtgctat actgtaattg
    17041 aaattttttt agtttttgcg ttagaactgt ttcctcagtc ctagcgcctt ttctttttga
    17101 taagtctgct tggtggagag tagtttgtaa acgattctga gaatcttatg agcacaagca
    17161 atgacggctt tcatcttgct tcctctttgg gaaattcgat tgtaaaactc taagcaggca
    17221 gagcctgctt agagttttac atatgctact atctttgtta tagctaactt aaattctcat
    17281 tta
//
LOCUS       DAVLYR010000020        17131 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLYR010000020 DAVLYR010000000
VERSION     DAVLYR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.020"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(5..760)
                     /locus_tag="VYO63_000617"
                     /pseudo
     CDS             complement(5..760)
                     /locus_tag="VYO63_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(898..2520)
                     /gene="groL"
                     /locus_tag="VYO63_000618"
     CDS             complement(898..2520)
                     /gene="groL"
                     /locus_tag="VYO63_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1671588.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2536..2820)
                     /gene="groES"
                     /locus_tag="VYO63_000619"
     CDS             complement(2536..2820)
                     /gene="groES"
                     /locus_tag="VYO63_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1671589.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3090..3485)
                     /locus_tag="VYO63_000620"
     CDS             complement(3090..3485)
                     /locus_tag="VYO63_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1671590.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3563..4324)
                     /locus_tag="VYO63_000621"
     CDS             complement(3563..4324)
                     /locus_tag="VYO63_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1671591.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4357..4983)
                     /locus_tag="VYO63_000622"
     CDS             complement(4357..4983)
                     /locus_tag="VYO63_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1671592.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4999..5316)
                     /locus_tag="VYO63_000623"
     CDS             complement(4999..5316)
                     /locus_tag="VYO63_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1671593.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(5313..5612)
                     /locus_tag="VYO63_000624"
     CDS             complement(5313..5612)
                     /locus_tag="VYO63_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1671594.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5862..6050)
                     /locus_tag="VYO63_000625"
     CDS             complement(5862..6050)
                     /locus_tag="VYO63_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1671595.1"
                     /translation="MKASTWVYDKGEWYYVSSSGAMLANDWVKDNGKWYYLASSGKML
                     RNTYTPDGYYVGNSGAWQ"
     gene            complement(6301..6975)
                     /locus_tag="VYO63_000626"
     CDS             complement(6301..6975)
                     /locus_tag="VYO63_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671596.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(6981..7427)
                     /locus_tag="VYO63_000627"
     CDS             complement(6981..7427)
                     /locus_tag="VYO63_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1671597.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7541..8044)
                     /locus_tag="VYO63_000628"
     CDS             complement(7541..8044)
                     /locus_tag="VYO63_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1671598.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8041..8403)
                     /locus_tag="VYO63_000629"
     CDS             complement(8041..8403)
                     /locus_tag="VYO63_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEV1671599.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8469..10328)
                     /locus_tag="VYO63_000630"
                     /pseudo
     CDS             complement(8469..10328)
                     /locus_tag="VYO63_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(10367..10816)
                     /locus_tag="VYO63_000631"
     CDS             complement(10367..10816)
                     /locus_tag="VYO63_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1671600.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10940..11656)
                     /locus_tag="VYO63_000632"
     CDS             complement(10940..11656)
                     /locus_tag="VYO63_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1671601.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12537..13952)
                     /gene="ply"
                     /locus_tag="VYO63_000633"
     CDS             complement(12537..13952)
                     /gene="ply"
                     /locus_tag="VYO63_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1671602.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13963..14295)
                     /locus_tag="VYO63_000634"
     CDS             complement(13963..14295)
                     /locus_tag="VYO63_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671603.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14366..14974)
                     /locus_tag="VYO63_000635"
     CDS             complement(14366..14974)
                     /locus_tag="VYO63_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671604.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14987..15445)
                     /locus_tag="VYO63_000636"
     CDS             complement(14987..15445)
                     /locus_tag="VYO63_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1671605.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15570..15969
                     /locus_tag="VYO63_000637"
                     /pseudo
     CDS             15570..15969
                     /locus_tag="VYO63_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16734..16994)
                     /locus_tag="VYO63_000638"
     CDS             complement(16734..16994)
                     /locus_tag="VYO63_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671606.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
BASE COUNT         5023 a         3876 c         3119 g         5113 t
ORIGIN      
        1 agtttcaatc tactataaat ttgatatgag aatagtcttt ctcaacatcc ttatccaaca
       61 agcatgctgt ggcgtccttc ttaacttaaa ccaggtcaat attctgggct tgggctgcat
      121 agacgcagcc acaataacat tgacgataga tatcatactc ctcacacatc tctactgaac
      181 gtttgtagcc ttgatttttc ttgaaatcgt tgggaagata gtggggcgtg taaatttttt
      241 gcacatcgat tccgatgcta ttgatagttt gagaattctt atgaggactg atggtcaagg
      301 ctgaaccaaa gtagtcaaag cccaagtcca tagccacttg cgctgtttta tccagtcggt
      361 agtcaaaaca aaccttgcaa cggtcgccac cttcgggctc ctcctctagc cccctaacta
      421 gttttcggta ttaattgggt tcgtagggag cttctaggta ctgaaccgta tttcctgtcc
      481 gctcattaaa atcactaaca aatttcttgg tgacatagac ccgcttatgg tattctgcct
      541 tgggatggat attagaattg gcaaaataga tggtcacatc tgcatacttg gtcaaatatt
      601 ctagtgtata ggtactacaa ggggcacagc aaacatgcac gagaatggtt ggccgctact
      661 catttttttc ccatgcttgt accattttct gcatgacacg gtcataatta atcttctgat
      721 tgggggtcat cttgcacaga atttcttcta catcgatcat gttcttctcc tttttctagt
      781 cttattttat catataagtt aggagagctc aaatcaattt agaagtgaat attataaaaa
      841 gaagggttag tcattccctc cttttgtgtt ttttataagt tgttttctat agaaagctta
      901 catcatcccg cccatcatac ttggatccat tgctggagct ggggctactg gttctggttt
      961 attggctacg actgcttctg ttgtcaaaat caagctggct acagatgctg cattttgtag
     1021 ggctgaacga ctcactttaa ctggatcaat gataccttga tcaatcatgt taacccactc
     1081 gccagttgct gcgttgaatc ctataccaag ctcagcattt ttcaaacgat cgataacgat
     1141 agatccttca aatcctgcat tgtgagcaat ttgacgaaca ggttcttcca aagcacggag
     1201 aacaatatta cgtcctgttg cttcatctcc tgtcaattcc aaggtagcaa cagctggaat
     1261 cacattggca agagctgttc caccacctgc aacaatacct tcttcaacag ctgcacgagt
     1321 agcgttgagg gcatcttcaa tgcggagttt catttctttc aactcagttt cagttgcggc
     1381 tccgacctta ataaccgcta caccacctga caatttggcc aagcgttctt gcaatttttc
     1441 acggtcaaat tcagaagttg tagtttcgat ttgagacttg ataaccgcaa cacggtgaga
     1501 aatcgcttca ggatttcctg caccttctac aataaccgtg ctatctttgt ccacggtcac
     1561 tctcgctgct tgaccaagag cttcaattgt cgcatctttc aactcaagac caaggtcttc
     1621 tgtgataact gttccgcctg ttaagatggc gatatcttca agcatggctt tgcgacggtc
     1681 accaaaacca ggtgccttga ctgctactac gttgaaggtt ccacgaatct tgttcaaaac
     1741 aagagttgga agagcctcgc catccacatc atccgcaata atcaagagtg gacgattgct
     1801 ttggagaatg ctttccaaaa gtggcaagat ttcttggata ttggaaattt tcttgtctgt
     1861 aatcaaaatg tacggatttt caaggtcagc caccattttt tcgctatctg tcaccatgta
     1921 ctgtgaaagg taaccacggt caaactgcat tccttctacg acttcaagct ctgtttccat
     1981 accacgtgac tcttcgatgg tgatgacacc gtctttgcca actttttcca ttgcttcaga
     2041 gatgtactca ccaacttttt cagaacgaga agatacggct gcaacttgag cgatagcttc
     2101 tttattggca acagggatgg cgttgttttt caaagcttca actgctgcgg caactgctgt
     2161 ttcaatccca cgacgaatac cgattggatt tgcacctgct gtgacgtttt tgattccttc
     2221 acggacgatt gcttgggtca aaacagttgc agttgtagtt ccatcacctg cgatatcatt
     2281 ggtttttgaa gctacttctg ataccaattt ggcacccata ttttcaaaat ggtcttctaa
     2341 ttcaatttct ttggcaatag tcacaccgtc attggtaatc aatggtgagc caaatgattt
     2401 ttcaagaacg acattgcgac cttttggtcc caaagttact ttaacagtat ctgcaaggat
     2461 atcgacacca cggaccatgg ctgaacgggc atctgatgaa aatttaattt cttttgacat
     2521 acttactttc tccttctatt cttcaatgat tgccaagatg ttagcttcgc ctacgatgat
     2581 gtacttttca tcgccatctt tgacatcaag acctgcgtgg gcttcaacta agacacggtc
     2641 tccagtttta acacttggag caaccaagtc accgttcaag gtacgaacac cttgtccagt
     2701 agccacaact tgggctgttt tggttttttc ttgggctgaa cctgcgagaa caaagcctcc
     2761 aacagtttgt tctttttctt ctacttttaa gaccacacgg tcccctaatg gtttcaacat
     2821 ctgatttcct ccataatgag atagatatta ttagcactct ttatacctga gtgctaattc
     2881 atagttctat tgtatcactt agtcagaaat aatcaagaaa aaagtctgac tttctcaaga
     2941 taaaaagcct gagaccaact cagacttttt aatgtttata ctcaatgaaa atcaaagatc
     3001 aaactaggaa gctagccgca ggctgctcaa agcattgctt tgatgttgtg gataaagcta
     3061 acgtggtttg aagagatttt cgaagagtat taaaatggca attcttcctc ttccaagact
     3121 aaatctgcca aatcttggcc tgcattattt tcacgcatgg cacgttgagc acgactttcc
     3181 aagagttgga atcctgttac aagtacttcg gtcacgtagt tcatttggcc atttttctca
     3241 aagcgacggg tacgcaattc tccatcaacg gaaatgagac tacctttggt tgcgtagctt
     3301 gccaaagttt ctgctagtct gccccatagg accatattga caaaatcagc ttcacgttca
     3361 ccgttttggt ctttgtaacg acggttgaca gcgatagttg ctcgcgctac cgacttgtca
     3421 ttgttggttt tgtgcaattc tggtgtagac gttaaacgcc caatcatgat aactttatta
     3481 tacatatttt cttcctccta cttatctatt cgtaggaaat caaaaaaagt tacagaaatt
     3541 tgtaactttt cgaggaaatt tcttattttt tatgaatcat gaaacctgtc gcctgttgat
     3601 tggccataat ggtcatatct gtaatctgaa cacggcgagg ctgactggtc acatagacta
     3661 ctgtgtctgc aatatcctga gcttgcaagg cttctattcc ttggtaaacg gacgcagctc
     3721 gctctttatc accatgaaaa cgaacagttg agaaatctgt ttcgacaatc ccaggctgaa
     3781 tggttgtcac cttgatatcc gttgcgatgg tatcaattcg cagtccatcc gaaaaggtct
     3841 taaccgcagc cttggtagct gagtaaacag cggcaccagc ataggcgtag attcctgcgg
     3901 ttgaccccat attgataata tgaccttgat tgacttttat cattgctggc aagaaacagc
     3961 gagtaaccgc catcagacct ttaacattgg tatccaacat ggtcaacata tccaactcct
     4021 catagtcttg atagggagcc aagtcaagag ccagtccggc gttgttgacc aagatatcaa
     4081 tcctccctat cgtttctaga atatcagagc aaacagtctt caccatgacc atatccgtca
     4141 catctagtgg aaaagtccag acggtttgat ttggaaagct tactgcaaac tccgacttga
     4201 ggatttctaa tctgtctatc cgtcgtcctg ttagaacgac atcctcaccc tgctccagat
     4261 aagcacgcgc aatcgcttca ccgattcctg aggttgctcc tgtaatcact acattttttg
     4321 ccatcttatt tccttctatc agatgctgac aagttcctag gcagtccagt gtttcgctgg
     4381 gtcgaacggt gttccgacaa cttggtcttc tgataattca agcaccccac gtttttgtgg
     4441 agcatttggc agatgcaatt cacgaggact gcacatcata ccaaaactct tttcaccacg
     4501 aagttcacct gggaaaatga gattcccttt tggcatcata gctccaggaa gcgcgacaat
     4561 ggttttcaac cccacacgcg cattgggagc tcctgcaacg atttgtacag tcttatcact
     4621 tgcgactgca acttggcaga tgttgaggtg gtcactatct ggatgggcta ccatctcaac
     4681 aatttcaccg acaacaaact taggttcctt atcattaaca atttcttctg taaaaccttc
     4741 cgcctgcaac tcttggttca aacgagcgac ttgctcatct gtcaaaaaga cttgaccgcg
     4801 ctctgcaatt tcaaataaac ttgaaacttc gaaaatattc caagccactg tttccccatt
     4861 atctttgaga aaaacacggg ctaccttgcc tttgcgctcc acatccagtt tggcatctcc
     4921 gctatttttc acgatgacca taaggacatc accgacatgt tctttattat atgtaaaaat
     4981 cattgtttcc tttttctcct atttcagtcc tgctaaaaag tcattgattt gttgcttgct
     5041 tttacggtcg cgattgacaa aacgaccgat ttccttgtcc ttttctagaa caacaaggct
     5101 agggattcca tatacatccc agagtttggc caaatctata tactggtctc tatccactcg
     5161 aataaaggta aactctggat tagtctcctc aatttctggt aaggcaggat agatataacg
     5221 gcaatcgcca caccagtccg ccacaaaaag gaagaccttc ttaccgtctt tttctactaa
     5281 agatgccaat tcttctaaac ttgctggttg tatcataaga cttcctcctc atagactagg
     5341 tcttcatttt catagacaaa agtataatga cggccatcct caaaaatgac gccaccaagc
     5401 aagctctcta gactgctttc atagacttga acatagaggg tcgcaatttc ccccatgtct
     5461 gaaaaatggt ctcgtacaat cgctgtcaac tcttcctgag tcttcatgag cttatggtca
     5521 tctgcaactt ttttcgtagc aagggcaagg ctcccaatac caagcagagc cagacctgcc
     5581 atccacattt ttttagcttt cataccattc attttaacac aaaaaaggct ttaggacaaa
     5641 tgaggaaaca gcaaaaaagc aagtaaaaag cctcttcctt taaggaaaag gacttcttat
     5701 actcaatgaa aatcaaagac caaactagga agctagccgc aggctgctca aagcactgct
     5761 ttgaggttgt agatagaact gacgaagtca gctcaaaaca ctgttttgag gttgtggatg
     5821 aagctgacgt ggtttgaaga gattttcgaa gagtattatt cttattgcca ggcgcctgag
     5881 ttaccaacgt agtaaccatc aggtgtgtag gtattgcgaa gcatcttacc tgatgaagct
     5941 agataatacc acttgccatt atctttgacc caatcattcg ctagcatggc tccagaagaa
     6001 cttacataat accattctcc cttgtcataa acccaagtgc ttgctttcat ggctcctgag
     6061 caattaaagg ttcaaaaaac tgtccaatat cattcatttt tttaaagcat tttacactac
     6121 atcatccacg ctgtaggaca tagatacatc aaacgtaaga gccaaaatat cgaaaaatat
     6181 acaactttta aaggcaacat ttcatttttt aaagtctaac aaccatagaa ctgaaatcag
     6241 agcaaggcat caacttccta ctcttagtac gtctattttg atggttgaag tgaagatttt
     6301 ttatttaacg tagacaagct ccaacttccc aaaagccaca tctccacgga taatcaaggt
     6361 tttgctggtt ggggctacag gagcgtcagc cttagctgca ccaaaggagg tttctacttt
     6421 caaatctaca cgccagtgtt gtggaacata gacggttgca ttcccgaagg ccacttcaat
     6481 atttaaagtt gcaaaatcac ctagcatctc tgcattatca tagtagattt tagcatcccc
     6541 aaaagcgact tccacttggt catctacgag atcttgatct tgcttataga aggtcccgct
     6601 accaaaagcg acttccttat ccgtgacgac tgtttttttc ccattgtacc accatttttt
     6661 ttcattccag aacttacttg aatgcgtcag ataaccaaca ccaagtacca ccaagatgct
     6721 agcccaaata agagaatgat tggtaactgg taacaagtca taagcgtaat ttgcaatgat
     6781 gagcgccagt aaacctgtaa aaactgccga agtgagatga cgtctaagga tggactcaat
     6841 ggacttataa gcaaaaaaaa caatacctag taaaggccat attttaccat ccaaagaagg
     6901 aattccaaaa ttcccttgca gcaagatcca agctgctaaa accaataaaa caataccaaa
     6961 tgctttcttt ttcatgtttt ttacctcatg tttcttagat tttcttttag gagggattgg
     7021 taatgccgtg agacatgaac ctccttgtgc gtctgataaa aggaaatggt gcccgttcct
     7081 gaaaaggact tgtccactga gtaaatctga cggatgtttg cgatcgttga cttggaaact
     7141 cgattaaaat agcgaggcaa gatagactcc aactcataga gcttgaggcg aacctcataa
     7201 gcttccttct ggttatgagc gtagatcttg ctcccttctg tttcaaagaa gagaatttct
     7261 gacaggtcta gataatattc acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac
     7321 tcttgcaaga gccgttgcaa gtctgcaatt tcatctgtca aagtgggggt cttgatgaca
     7381 atttcagtct cctctaaatt gccgtcaatc tcaattcgta acttcatcac atctcctttc
     7441 tgactctact atatcaaagc taagttagga atacaagggc aaaaaagcaa gtggtcaatt
     7501 tgaagcagta agtggttgta ggactagctt aacttacatg tcagaatagg aagaaaagga
     7561 atccccacac cagaccacaa gcatagccaa ccaagacatc cttgggataa tgaacacccc
     7621 ctaaaaccct caccaacccc agcaaagctg aaaagatcaa gcatactaag ccaataggta
     7681 gactagcatg cagacaggcc atggagatga tagttgcaga aaagacatgg cgactgggca
     7741 tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat atcccaagtt tcataaggtc
     7801 tcgggtgatt gattttctta cgaaacaggg acaaggccac aaaacctgat gcaggaataa
     7861 gcacataaac cccaacttgc tgaccaagtc ctagttgcag gtaagtggtg actagcaaga
     7921 tcagatagac caggggcatg gcgactgtca tcaaacgatt gaaactgcgt aataggaata
     7981 aaagaattgg acgactagtt agtctggagc tgatatttcg ataccattct tgataatttt
     8041 tcatttatgc ttctcatact tttctagaac ttgttgagat gttttcccct tggcaagcca
     8101 atccactagc ttgtccaaac gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat
     8161 atggacaccg caaatcttcc ctgtaattgc aattcgttca gggacataat atggtgcttg
     8221 tagaaaaaaa tctccgtaag taacatccct atccagacaa gcaagaacgt cactcacttc
     8281 ataccctgtc aaccaactgg tcacttggtg aactgactct gcctttccac cctttcgctc
     8341 aacttttgca atcaaggcta agtagacagc agcaaatttc atattgtata atttctgact
     8401 catacttctc tctttctatg tgtactagta taaataagaa aaagaaggcc gtcaagcctt
     8461 tttttgattt attcttctgc ttcatcttct gtaaattgac tattgtacaa gtcagcgtag
     8521 aagccacctt gcgccatcag ttcctcatag ttgccttgct cgatgatatt tccatctttc
     8581 atgaccaaga tcaagtctgc atttcggatg attgacaagc ggtgggcaat gacaaaggat
     8641 gtgcgtcctt ccatcaaacg gtccatggct ttttggatca attcctctgt ccgtgtgtca
     8701 acagaagaag tcgcctcatc caaaatcaaa agcgatgcat ccttaagaag ggcacaagca
     8761 atagtcaata gttgtttttg tcctacagac aaggtcacgg tgtcatccaa gatggtatca
     8821 tagccatctg gcaaggtcat aataaagtgg tgaattccca cagccttact agcttccatc
     8881 attcgttcat cactaatccc tatttgatta tagatgagat tgtctcgaat agttccttca
     8941 aagagccagg tatcctgcaa gaccattgaa aaggcatcat gcacttctga acgcgtcata
     9001 gccttggtat ccacaccatc aatgcgaata cttctcttat caatctcata gaatttcatc
     9061 aaaagattga caatggttgt cttaccagcc ccagtcggcc caacaatggc aaccctttga
     9121 ccagcatgag ctgtcacaga gaagtcatag tcttgaacat tgacaccgtc caccagaatt
     9181 tctcctgctg acacgtcgta gaaacgtgga atcagattga ccagagttga tttaccagaa
     9241 cctgttgacc caataaaggc cactgtttga ccagtttctg ctttaaagct aacatgttca
     9301 ataactgcct ccgaatttgc cgcatagcgg aaggtcacat ccttaaactc gacctgacct
     9361 ttgaagtttt catcagtcag ctgcacttga acagggtttt ggatagaaga atgcaaatct
     9421 aaaacttgat taatccgctt agcagagacc atagttcggg gaagaacgat gaagagtgct
     9481 cccatgagaa ggaagcccat gacaacctac atggcataag acatgaaaat aatcatgtca
     9541 ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa tcacataggc cccaatccag
     9601 taaatcgcta cactcaaacc acttgaaatc cccatcatga taggattcaa aatagccata
     9661 agacggttga caaacaaatt caaacgggtc aattcatcat ttactgctgc aaatttttca
     9721 ttttgataat cctctgcatt gtaggcacga acgacacgaa tacctgttaa actctcacga
     9781 gtgatactgt tcagtttatc tgtcaacccc tgaatcaagg actgttttgg aaaggctagc
     9841 gtcatcaaaa cggtcgtcat caggacgttg acaatcacta ccacaagtac ggcccagagc
     9901 cagtattctg aatgacctaa aatcttccca atagcccaga tagccataat tggaccacgc
     9961 gttaccactt gcaagcccat agtaatcaac atttgaactt gagtaatgtc attggtagta
    10021 cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg tctgcgagta atccaaaact
    10081 cggttaaaaa tatcacttct cagcctacta gtataagaag ccgccactcg ggatgcaaaa
    10141 aatccaactg caactacgga caagaaggca agaaaggaca ttcccatcat catgcttgcc
    10201 ggctgccaca actcatctaa attagtttct tgactaccta gcaaatccgt aattttcgag
    10261 atataggtcg gcacttccaa ctctagatag accgaaaagc aagtaaagag aatggctagt
    10321 aaaatcatcc cccattcttt tctactaatt cttttggcta atttctttat tctctcctcc
    10381 tattcccttg atattttgcc tgtagttgac cgagaacctt ctcaaaaatc agtaattcat
    10441 cttcatcaat atcttccatc aactgcttgt ctatgcgttc aaaaaaagcc ttaacctgtt
    10501 gcatctgaga acgtgctttg tccgtcagac gaacaaactt agcccgctta tcaacaggac
    10561 tcgcctccaa ttccaccaaa ccattttgca ctatacgctt aaccagatta ctagcaacag
    10621 acttggtaat attgagttcc tgatcgatat ctttaatcaa gaccaagtct tggtttttct
    10681 cgcgattatc caaaaaatgc acaacctgac cttgcggccc acccataaat tcaatgccgc
    10741 aacgtttggc ttccttttgc accatcaggt gaatttgatg accaaaacgc ttaaagacta
    10801 acatcggttt acccataatc tcccccttct aaataaaaat agttctctgg agaataatta
    10861 aatttctatg agaactattt tcttgattaa aaaaatccca agtgattttc tcacttagga
    10921 tcatgttcta taggttaaat taaaacccat ctacgttcgt ataaatcttt tggacgtctt
    10981 cgtcgtcttc aagaacgctg taaagttttt caaaggtttc aaggtcttcg cctgacaatt
    11041 ccacttctga ctgaggaatc atttccaatt cagtcacttg gaattcttca ataccagact
    11101 cacggagggc aacgatagcc ttgtgaaggt cagttggcgc tgtgtaaact gtgattgtac
    11161 cttcttgtgc ttctacgtca tccacatcca catccgcttc gagcaattgc tcaaagactg
    11221 cgtccgcatc ttcacctcca aatacaataa cacctttgtt gtcaaagagg taagaaacag
    11281 aacctgaagc gcccatgttt ccgccgtttt taccaaaggc tgcacggaca ttggctgctg
    11341 tacggttaac gttagaagtc aaagtatcca caattagcat agagccattt ggcccaaaac
    11401 cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc tttggcttta tcaatcgctt
    11461 tatcgataat gtgttttggc acttgggctt gtttagcacg gtcgataacg aatttcaaag
    11521 ctgagtttga ttctggatct ggatcacctt ttttagctgc tacatagatt tctacaccaa
    11581 attttgcata tactttagag ttggctccat ctttagccgt tttcttggct acgatattgg
    11641 cccatttacg tcccattagg aatctccttt tttcacattt taatctttct tattataaca
    11701 caagtttttt tgattttcac tagaggaaat ggattttatt agcaaatcaa gctaggatag
    11761 cactttacct gctaagatgg tcttgccttt ctatctttat caacagacac tcatccacat
    11821 tcaaaaaaca aactagacca ttatctgcaa atagaaagtt tcagccaagt ttgacaaagt
    11881 cagctcaaat tactgtttga agtttgtaga tataagcgac aaaaacaatc atactgcacc
    11941 ttttgttgac agtctactcc agacatatca tagttcaagt aaatactttg aaattcaaca
    12001 gttcttatag gcgctattgt attctaagaa atcaatagaa gagtttctaa gcaaacctct
    12061 aatactcaat aaaaatcaaa gagcaaacta gaaagctagc cgcaggttgc tcaaaacact
    12121 gttttgaggt tgcggatagg gctgacatgg tttgaagaga ttttctaaga gtataattta
    12181 cgtgttccta agatggagaa gttagactag tacactggca cttctaaacc attgctagca
    12241 attgatttgt tcatatttaa tttcattttt tccataaatg ggtattagat ataaacagca
    12301 aaatatttcc gatacgtgtc attcttgaat ttccaatcat ctaaaacaag taaaggataa
    12361 tcaatcccct gtatatcaag gaattggcta ccctttttac ttttttacac atcctgtttg
    12421 atagactcat tttaacatca cgagcatact ccaatggaaa tcgctaggca agagataaac
    12481 tttcagatat ccgcagagag atcatcgcct ctttttgtcg caagcattct cctctcctag
    12541 tcattttcta ccttatcctc tacctgagga tagagagttg ttccccaaat agaaatcgtc
    12601 cgcttacgca ctagtggcaa atcggttttt tcataaaccg tacgccacca ttcccaggca
    12661 agcccggtac actctctaat tttgacagag agattacgaa cattcccttt taaaggaata
    12721 ctagtggtaa agtgagccgt caaatcctgc ccatttctgt cccaagcctt aggagtcaag
    12781 acttccttac cttgatgatc ataggataat tcatcccaag taatataata ttgggcaaca
    12841 taggcaccac tatgatccag cagtaaatct ccgtttctgt aagctgtaac cttagtctca
    12901 acatagtctg tactgttttg aaaggtcgca actacattgt cacgtaaaaa agaagttgta
    12961 taggaaatcg gcaagcctgg atgatctgct gtaaagcgac tgccttcttg aatcaagtcc
    13021 tctaccatat ccaccttgcc tgttacaact cgggcacccg aacttgggtc gccccctaaa
    13081 ataaccgcct tcacttctgt attgtccaaa atctgcttcc actctgtctg aggagctacc
    13141 ttgactcctt ttatcaaagc ttcaaaagca gcctctactt catcactctt actcgtggtt
    13201 tccaacttga gatagacttg gcgcccataa gcaacactcg aaatatagac caaaggacgc
    13261 tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta cagtatcttg aaacacatct
    13321 cctggatttt taacagcgtc tacgctgact gtataataaa tctgcttaaa attaacaatc
    13381 tgaatctgct tttcacctga atggacagag ttaaaatcaa tatcaagaga attccctgtc
    13441 ttttcaaagt cagaaccaaa cttgaccttg agttgttcca tgctgtgagc cgttattttt
    13501 tcatactgca ttctagctgg gacattattg acctgaccat aatcttgatg ccacttagcc
    13561 aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg ggtcttccac ttggagaaag
    13621 ctatcgctac ttgccaaacc aggcaaatca atactataag tcatcggagc acgatcaacc
    13681 gcaagaagag tgggattatt ctctaacaag gtctcatcca ctacgagaag tgctccagga
    13741 tagaggcgac tgtcgttggt agctgttaca gaaatatcac ttgtatttgt cgacaagctc
    13801 cgcttctttc tttcgataac aacaaactca tcgggtagct gattaccctc tttgatgaaa
    13861 cgattttcaa tactttctcc ctgatgggtc aagagtttct ttttatcgta attcatagct
    13921 agtataaagt catttactgc tttatttgcc atcttctacc tcctaataag ttcctggatt
    13981 gagttgcata aactcagact tgttcagcga aatcagccgt ggttggacta agtgatccaa
    14041 aatttcctcg tacaattctt ctgagacatt gcgtcgccgt ctggctagat aagaagtcgg
    14101 aataaccgta ttatccaaca taaatacctt atctaagtca atcaaggttg gtcttgtaaa
    14161 aggattacga gctagatccg gctcttctat cataaagttc ttgaccaaac gtctggtcaa
    14221 gagagctggt ttgaaggtct gatttttaac caactctttg tttttagtca tgctgttgtc
    14281 aatacagata tacatatgat tcttcacagc caaatcgcta ctaatagtcg gaaaaggcaa
    14341 ataaagagct acaacatctc ctctcttaat caagcaagag cacccccttt tctcctaatg
    14401 taacatagac aggattgacc aagtcttctg attgactcag aatttccaaa gtttgagttt
    14461 ggcgcgctgt caatttagta gcatcttgtc tcttcaatac aaaatgcttg tcgccaataa
    14521 ccttgacaat ataatccttc tccaaagctg actggtaaat ccacatcaga tgttgtctgt
    14581 cctgagaact caagagagaa ggattttcaa gcctcccgat agtctgataa aaatcaaaaa
    14641 caggagctaa ctcctgccaa tctgattggc tagttgtcaa ggctagaaaa agggctttgc
    14701 gagctgatac ttcttggtta gccttgagag ttactttccc ctccaagttt tttagaaatc
    14761 gggaaactcc agaaagcaaa tttttctcta actgcgagaa ataaaaacct ttcgttccca
    14821 gacataagtc tttcatgtcg ctttctctag caaataagag ctcaaacatt tgatagtaaa
    14881 agaaaaatat ctggcactgg gtcgcgctca tcttttcctt atcggcttct ttttttaacc
    14941 agagcaaggg cgacaggtaa ctggattgag acatttcctc tacctcctac tcttttttaa
    15001 ctggagcatc tgcactagct gccacttctt ttgactggat actttcccac tggttaatct
    15061 cctctgagat aagaccttcg catgtcttga caaatagggc aaaagccttg gtctttcctg
    15121 catatttctc cgtttggcat tgatagagga atttttcttt ctccaggagt tgcgcagttt
    15181 tttggtaaga aatccaattt tcctttgcat tatacaaatt gataatcccc tcacacagca
    15241 agccgagact ggataaggca accgaaatca aacggtagcg atcacctggc ataggaatag
    15301 cacaaaagac agctatgagg aaacctgcca cgatttctgt tatttttaat accttatagc
    15361 gcctacgatg ttgaacgctt ttctttaaaa aatgagctat ctgtacgtct aatcgctctg
    15421 tcaggtacat ttcttctggc gtcatattcg taactccttt catttacttt gataaccata
    15481 gtataacata tttgaatatc aaagtatagt aaaaactgga ctataactta ttagacttcc
    15541 tgcgaaacaa aatatggtat agtagttcta tgaatgatga agcaagtaaa caactaactg
    15601 atgcacgatt taagcgtctt gttggtgttc agcgtaccac ttttgaagag atgttagctg
    15661 tattaaaaac agcttatcaa aaaagtcgga caagctggta aaatcttggc tgacagtaat
    15721 tatcaagggc tcatgaagat atatcctcaa gcacaaactc cacgtaaatc cagcaaactc
    15781 aagccactaa cagttgaaga taaagtctat aaccatgcgc tatctaagga gagaagcaag
    15841 gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctgtcgaaat
    15901 catcgtaaac gcttcggatt acgaatgaat ttgagtgctg gtattatcaa tcatgaacta
    15961 ggattctagt tttgcaggaa gtctattata tatatgcaca gacatatgaa atctttttga
    16021 tacagccttc tttttacata gagagacagc agttatgata tatttatcca ttcacaacat
    16081 cttctgtacc aatactacaa taattggtac attttgatta cacaatcgtt tcaaaaaata
    16141 tgatggaaga ctgagtattt tatagtaatt cccttcctat caactagatc aaccagttga
    16201 gtgttgaatg ttgaaacacc ctagcctcag caaaggacta tgatcagcat ttcctagcca
    16261 ataggacgct ccaaatcacc tattctaagt ccatcactag cttggtcagt aagatacaga
    16321 ctgcaaggca agactgacgt ggttgatgag attttggaag agtattacaa tcatagattc
    16381 accagtagac ttgaccgcag atttaactat agtagattga aactagaata gtacacttct
    16441 gcttctaaaa cattgttaga aatcgatttg actgtcctga tcaatttgtc ctgttctcgt
    16501 ttcattttac tatactaaaa aaccacctga aacaaatcaa gtgactagag gataaaaaca
    16561 aagactgggc aaaaagtttt gaaaagcaaa aaacgcataa tatcaggtct tgaaaaacct
    16621 tgatactata cgtttgattg tgggaagatt gacttcattt tctcctgaaa ttgaagtttt
    16681 ttctagtttc caggtataga cgaattcaat atagccatct cgctacaatt attttaacca
    16741 tcgaaaaaat ttttcagaat ttcaaaaatt tcattttgat ccaatgaaat gtcattattt
    16801 tgaagaatag ttgacttcac aggctctaat tctatcacaa agcggtgcat gtctgctttt
    16861 accgcactac ttgatgttat agccgataac ttttcatctc ctagcaagga agctgtcaaa
    16921 cgggtaaggt cattcaaatg ctttttaatg gacttactta aaccttgagc acctgtggca
    16981 gagcgctccc tcatatccag ccaagcttta gccttaaaaa taattaatac tcaatgaaaa
    17041 tcaaagagca aactaggaag ctagttgcag gctgctcaaa acactgtttt gaggttgtag
    17101 atggaagctg acgtggtttg aagagatttt c
//
LOCUS       DAVLYR010000021        17044 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLYR010000021 DAVLYR010000000
VERSION     DAVLYR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.021"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            336..1382
                     /gene="pheS"
                     /locus_tag="VYO63_000639"
     CDS             336..1382
                     /gene="pheS"
                     /locus_tag="VYO63_000639"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1671607.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1382..1891
                     /locus_tag="VYO63_000640"
     CDS             1382..1891
                     /locus_tag="VYO63_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671608.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1968..4373
                     /locus_tag="VYO63_000641"
     CDS             1968..4373
                     /locus_tag="VYO63_000641"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1671609.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNESAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4441..5442)
                     /locus_tag="VYO63_000642"
     CDS             complement(4441..5442)
                     /locus_tag="VYO63_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1671610.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTDSGIAPVTVIVKKSYDFYTKAETFHTTRFGTIVLHSRKQDIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5598..6087
                     /locus_tag="VYO63_000643"
                     /pseudo
     CDS             5598..6087
                     /locus_tag="VYO63_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6171..6437
                     /locus_tag="VYO63_000644"
     CDS             6171..6437
                     /locus_tag="VYO63_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1671611.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6698..8947
                     /gene="metE"
                     /locus_tag="VYO63_000645"
     CDS             6698..8947
                     /gene="metE"
                     /locus_tag="VYO63_000645"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1671612.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLSAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFTTERVGENAELRARIAG
                     LTDADYTRLPVFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9011..9877
                     /gene="metF"
                     /locus_tag="VYO63_000646"
     CDS             9011..9877
                     /gene="metF"
                     /locus_tag="VYO63_000646"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1671613.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELVPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10562..12775
                     /gene="pnp"
                     /locus_tag="VYO63_000647"
     CDS             10562..12775
                     /gene="pnp"
                     /locus_tag="VYO63_000647"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1671614.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12791..13408
                     /gene="cysE"
                     /locus_tag="VYO63_000648"
     CDS             12791..13408
                     /gene="cysE"
                     /locus_tag="VYO63_000648"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1671615.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13420..14304
                     /locus_tag="VYO63_000649"
     CDS             13420..14304
                     /locus_tag="VYO63_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671616.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14391..15734
                     /locus_tag="VYO63_000650"
     CDS             14391..15734
                     /locus_tag="VYO63_000650"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1671617.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15727..16113
                     /locus_tag="VYO63_000651"
     CDS             15727..16113
                     /locus_tag="VYO63_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1671618.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16117..17001
                     /locus_tag="VYO63_000652"
     CDS             16117..17001
                     /locus_tag="VYO63_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1671619.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         5056 a         3335 c         3897 g         4756 t
ORIGIN      
        1 tgttctcatc ttatttcaat ccgctataaa gttagataag aataagctaa caagcagaag
       61 taagataggt cttgcaagaa tggtaaaaat ctgataaact aagtagtaat tgaataggaa
      121 tctgaaagac tagtagccca agggaagtat atcagggagg agagccgtga ctgcaagctc
      181 tctatatgaa agctgggtga attcacttgc gcatggattt agaaatgaag gtctgacttg
      241 tcagatgaaa acggatggta ccgcgtgtca acgctccgag tggagttttt ggcatgtggt
      301 tttcttttta tctacgagag actgatggag gaaatatgtc aactattgaa gaacaattaa
      361 aagcgcttcg cgaagaaacg ctggctagct tgaagcagat tactgctgga aatgaaaaag
      421 agatgcaaga tttgcgtgtc tctgtccttg gtaaaaaggg ttcgctcact gaaatcctca
      481 aagggatgaa agatgtttct gctgagatgc gtccaatcat cgggaaacac gtcaatgaag
      541 ctcgtgatgt cttgactgct gcttttgaag aaacagctaa gctcttggaa gaaaagaaag
      601 tcgcggctca actggctagc gagagtatcg atgtgacgct tccaggtcgt ccagttgcga
      661 ctggtcaccg tcacgttttg acacaaacca gtgaagaaat cgaagatatc ttcatcggta
      721 tgggttatca agtcgtggat ggttttgaag tggagcaaga ctactataac tttgaacgta
      781 tgaaccttcc aaaagaccac ccagctcgtg atatgcagga tactttctat atcactgaag
      841 aaatcttgct ccgtacccac acgtctccag ttcaggcgcg tgctatggat gcccatgatt
      901 tttctaaagg tcctttgaag atgatctcgc cagggcgtgt cttccgtcgc gatacggacg
      961 atgcgaccca cagtcaccaa ttccaccaaa tcgaaggctt ggtagttggg aaaaatatct
     1021 ctatggctga tcttcaagga acgcttcagt tgattgtcca aaaaatgttt ggtgaagagc
     1081 gtcaaattcg tttgcgtcca tcttacttcc cattcacaga gccatctgtt gaggtggatg
     1141 tttcttgctt caagtgtggt ggagaaggct gtaatgtatg taagaaaaca ggttggatcg
     1201 aaattatggg ggccggtatg gttcacccac gtgtccttga aatgagtggt atcgatgcga
     1261 ctgtatactc tggctttgcc tttggtcttg gacaagagcg tgtagctatg ctccgttatg
     1321 gaatcaacga tatccgtgga ttctaccaag gagatgtccg cttctcagaa cagtttaaat
     1381 aatgattaga aaagtagaaa tggcagatgt tgaggtgttg gctaaaattg ccaaacaagc
     1441 ctttcgtgaa acctttgcgt atgataatac ggaagagcag ttacaggaat actttgaaga
     1501 ggcttatagt ctgaaaactt tgtcaactga gttgggaaat cctgactctg aaacctattt
     1561 cattatgcat gaggaggaga tagctggttt tctcaaagtc aactggggaa gtgctcaaac
     1621 tgagagagaa ttagaggacg cttttgaaat tcaacgcctc tatgtgctac aaaaattcca
     1681 aggatttgga ctaggtaagc aactgtttga attcgcactt gaacttgcta caaaaaatag
     1741 tttttcttgg gcttggttag gtgtttggga gcataataca aaagctcaag cgttttataa
     1801 tcgatatggt tttgaaaaat ttagccaaca tcattttatg gttggtcaaa aagtagatac
     1861 ggattggtta ctgagaaaga aattaaggta agaagaggat tcttctattg aaatgatagg
     1921 aaaggaaaag aactagagtg acaactgtca ttctggagaa ctaaattatg cttgtatctt
     1981 ataaatggtt aaaagaattg gtggacattg atgtgccatc acaagagttg gctgaaaaaa
     2041 tgtcaactac aggaatcgag gtagagggtg tcgaatcacc agctgctggt ctctcaaaaa
     2101 ttgtcgtcgg tgaggtcttg tcttgcgaag atgtgccaga gactcacctc catgtttgtc
     2161 aggttaacgt tggcgaagaa gaagagcgtc agatcgtttg tggtgcccca aatgtgcgtg
     2221 ctgggatcaa ggtcatggtg gctcttccag gagctcgtat cgctgataac tacaaaatca
     2281 aaaaaggaaa aatccgtggt ttggagtcac ttggaatgat ctgttcactt ggtgaattgg
     2341 gaatttctga ctcagttgtg cctaaggaat tcgcagatgg catccaaatc ttgccagaaa
     2401 atgccgtgcc aggtgaggaa gtcttttctt acctagactt ggatgatgaa atcatcgaac
     2461 tttccatcac accaaaccgt gcagatgccc tttctatgtg tggagtggct cacgaagtgg
     2521 cagccatcta tgacaaggca gtcaacttta aagaatttac tctaacagaa actaatgaat
     2581 ctgcggcaga tgccctttct gtcagcattg ggacagacaa ggcgccttac tatgcagctc
     2641 gtatcttgga caatgtgact atcgcaccaa gtccacaatg gttgcaaaac cttctcatga
     2701 acgaaggcat ccgtcccatc aataacgttg tagacgtgac aaactacatc ctgctctact
     2761 ttggtcaacc tatgcatgct tttgacttgg acacatttga agggactgac atccgtgtgc
     2821 gtgaagcgcg tgctggtgaa aagttggtga ccttggacgg tgaagaacga gacttggctg
     2881 agacagacct cgtgattaca gttgctgaca aaccagtagc ccttgccggt gttatgggtg
     2941 gtcaggctac agaaatttct gaaaaatcta gtcgtgttat ccttgaagct gctgttttta
     3001 atggcaaatc tatccgtaag acaagtggtc gcctgaacct tcgttctgag tcatcttctc
     3061 gctttgaaaa aggaattaat gtggcaacag ttaatgaagc ccttgatgcg gcagctagcc
     3121 tgattgcgga acttgcaggt gcgacggtgc gtaagggtat cgtttcagcg ggtgagcttg
     3181 atacttcaga tgtagaagtt tcttcaactc ttgctgatgt taaccgtgtc ctcggaactg
     3241 agctgtctta tgctgatgtc gaagacgtct tccgtcgtct tggctttggt ctttctggaa
     3301 atgcagacag ctttacagtc agcgtaccac gtcgtcgttg ggatatcaca atcgaagctg
     3361 atctctttga agaaatcgct cgtatctatg gatatgaccg tttgccaacc agccttccaa
     3421 aagacgatgg tacagctggt gaattgactg tgacacaaaa actccgccgt caagttcgta
     3481 ccattgctga aggagcaggt ttgacagaaa tcatcaccta tactctaaca actcctgaaa
     3541 aagcagttga gtttacggct caaccaagta accttacgga actcatgtgg ccaatgactg
     3601 tggatcgttc agtcctccgt caaaatatga tttcaggtat ccttgatacc gttgcctaca
     3661 acgtggctcg taagaataaa aacttggccc tttacgagat tggaaaagtc tttgaacaaa
     3721 caggtaatcc aaaagaagaa cttccaaatg aaatcaacag ttttgccttt gccttgacag
     3781 gcttggttgc tgaaaaagat ttccaaacag cagcagttcc agttgatttc ttctatgcta
     3841 agggaatcct tgaagcgctc tttactcgct tgggactaga agtgacctat acagcaacag
     3901 ctgagattgc cagccttcac ccaggacgta cagcagtcat ttcactcggt gaccaagttc
     3961 ttggttttct tggccaagtg catccagtca ctgccaaggc ttacgatatt ccagaaacgt
     4021 atgtagctga gcttaacctt tcagccatcg aaggggcgct ccaacctgct gttccatttg
     4081 tggaaatcac gaaattccca gcagtcagcc gtgacgttgc ccttctcctc aaggcagaag
     4141 taactcacca agaagttgta gatgctatcc aagctgccgg cgtgaaacgt ttgacagata
     4201 tcaaactctt tgacgtcttc tcaggtgaga aattgggact tggtatgaag tccatggctt
     4261 atagcttgac cttccaaaat ccagaagata gcttaacgga cgaagaagtc gcacgctata
     4321 tggaaaaaat ccaagcatcg ctcgaagaaa aagtgaatgc agaagtgcgt taaacgatag
     4381 taggatataa attccccctc tagtttatga acactagagg ggtgatttta tataaaataa
     4441 ttaaaatgtg atttctgtaa aaatacatct atgatcagag ttttgaaaac ttacaatgtc
     4501 taaatcttta acatagtagt ggtttttagg caataaaata tgatctatcg ttgcattaaa
     4561 aagttttgga ctttggctat tccaagttcc tctttcaaaa ggtggcagtg catttaatgc
     4621 gtccctatga gagcttattt ttgcaagtgc tccatgacgc atagttgcat taaaatcacc
     4681 tgcaataata gcctttggat attttgaagc caattgatta tgaatgatgt ttaagtcttg
     4741 cttccagatt tccattaaac ctggcagagg aggagcagta tgcaaagcaa tgatatctgg
     4801 tatatcttgt tttctcgaat gtaatacaat tgtcccgaac cgtgttgtat gaaaagtttc
     4861 agcttttgta tagaaatcgt aacttttctt gacaatcaca gtcacaggag ctattccact
     4921 gtcagtaggt ggagaagtaa aaatatcata gttggccata gaaagtccaa cttgatgaaa
     4981 caatagtttg attctctggt tttcttgctc acctctgata ttggtagcta gttcagggaa
     5041 tatagccata tcggcgtcaa aatggctaaa aattcgctca atatgttgtg cttctatttg
     5101 attagcgacg ttccaagtta ctaattttaa ggttttagtc ttatttgatg cggaagaaag
     5161 ggttttatct gttccgaata atagtaatat gagcgataat aagagcaaaa tgccagagta
     5221 aaaccgtatt tgtttatttt tcttgtaaaa agatagggaa aaaaatacga aacttaaaat
     5281 ggcagccata gttgcagtag ctaaggggaa ggcataaatc tgtgccaaga taggtctggt
     5341 agatagccaa atattaagat tagaagatgg aaaaaaagaa taagttatga tagggataac
     5401 aaagagaatg accgaaaggg cagagagaaa tgttttaatc attgtgtgag tacctcaatt
     5461 tgcaatggtt gctcggagct aatgtgactt tgtgacttta cttgattgat attctttttc
     5521 ttgtcttcct tatagattaa gtacaaccca agtaaaaaaa gtccaattta agattgaaat
     5581 gattaaaagg cacagagatg caagacaatt atactacaaa aggtaaacat ttgacaatcg
     5641 atagccgtcg cttaatcgaa agatggaaaa aagaaggaaa atcaaataga gaagttgcct
     5701 ctctacttgg aaaagctcct caaactatcc acactgaaat caagcgtggg acagtccgaa
     5761 aatgtcttgg aaaagggcgc ttcaaagagg tttattctgc cgactacgct caacagtctt
     5821 atgaaaacaa tcgcaagcgc tcggtcaaga aatcaagctt gaccaaggaa ctaaaggaaa
     5881 agattctcca ctatacataa ccaaaaattt ttgcctgaaa tgatggtgat ggctaaaggg
     5941 gttaacgtgg gaatttcaac catttactat tggattcatc atggaaagtt ggggttaagc
     6001 aaacaggatt tgctttatcc tagaaaagga aaatctgtta agaaacaagt tagtcccaat
     6061 tttaaacccg ctgatcaatc tatcgaatca taattcgctc atttttttaa aaatacatag
     6121 acgtaaaaaa aagagggtta agtgatgaag aatagtgcta ttgggagtaa ttggaaggat
     6181 gtccatctga gctctttagc aaggaggaaa tccttgaaag tgatatgcga gtggctatca
     6241 tgagcgagtt gattgaggct aggtatgaac aaggaatcag tcagaaaaag ctggaagaag
     6301 tcagtggaat aagccagcct gtcatagcta ggatggagac aggaaagact agtcctcagt
     6361 tggatacagt cttaaaagtc ctagccagtt taggaaagac actagcaatc gtcccacttg
     6421 aacagggaaa aagttgacta gaatcagtta tagtcttttc taataacaag ccatagtcac
     6481 ttataagaat tactaataac gcgtttgagc attctaacta aaatacagct ataaaacaag
     6541 ctatacaaag gatttgagac gtttgcttca agtctttttc ttttgtccga aacagatata
     6601 tagtttcaaa ctatatcaaa ccctaatttt ttacaattat acagacgctt tcttttctgt
     6661 taagatagtt tcaacaacaa attttggagg acacatcatg tcaactacaa tcgtcggttt
     6721 ccctcgtttg ggcgaattcc gtgaattaaa atttacaact gaaaaatact ttagaaaaga
     6781 aatctcagag gaagaacttt tatcagcagc aaaagacttg cgtgctaaac actggaacat
     6841 tgtcaaagaa aaaggcatca ctgaaattcc atcaaatgac ttttctcact atgataactt
     6901 cctagatgcg gctttccttt tcaacgtggt gcctgcatcc gttcaaaact tggacttgtc
     6961 tgaccttgag cgctacttcg ctttgggacg tggttaccag ggagaaaaag gggacgttcg
     7021 cgcccttcca atgaagaaat ggttcaacac taactaccac tacatcgttc ctaaatttga
     7081 aaaagacact caagtaaaat tggctggtca caagattttc gatgagttcc aagaagccaa
     7141 agaacttgga ttgaacactc gtcctgttct tgtaggtccc ttcactttcc ttcaattgtc
     7201 agactttgaa gaaggcgtga aagcagacga cttcgtagat agcttagtgg ctgcttacca
     7261 agaagttttt gctaaattgg ctgaacttgg tgcgactcgc atccaattgg atgaagcggc
     7321 tcttgtaaaa gacttgacag ctgaagaaaa agctctcttc ttgaaccttt acaacaaact
     7381 cttggctgac aaaaaaggtc ttgaagtctt gcttcaaact tatttcggtg atgttcgtga
     7441 cgtatacgct gaccttgtaa acttaccagt agatgctatc ggtcttgact ttgttgaagg
     7501 taagaaaact cttgaactcg ttaaaggtgg cttcccagct gacaagactc tctatgtagg
     7561 tattgtcaat ggtaaaaaca tctggcgcaa caactacgaa aagagcttgg ctgttcttga
     7621 gcaaatccca gctgaaaaca tcgttttgac aagctcttgc tcacttcttc atgtgccatt
     7681 tacaactgct aatgaagaat ttgaaccagc actcttgaac cactttgcct ttgcagttga
     7741 aaaattggat gaaatccgtg acttggatgc tatccgcaat ggtcaaggtt cagaagctct
     7801 tgcagccaac aaagaactct ttacgactga gcgtgttggt gaaaatgctg aacttcgtgc
     7861 gcgtatcgct ggcttgacag acgcagacta cactcgtttg ccagtctttg cagaacgtga
     7921 agctatccaa gaagaagctt tcaaacttcc agctcttcca acaacaacga ttggttcatt
     7981 ccctcaaaca aaagaagttc gtgccaaacg tttggcttac cgtaaaggtg aattgtctca
     8041 aaaagagtac gatgctttcc ttgctgaaac gattgacgaa tggatcaagt ggcaagaaga
     8101 tattgacttt gatgtccttg ttcacggtga atttgagcgt aatgacatgg ttgagtactt
     8161 cggtcaaaac ttgtcaggat acctcttctc taaaaatggt tgggtacagt catacggtat
     8221 gcgtggggtg aaaccaccaa tcatctgggg tgatgtcact cgtcttaacc ctatcactgt
     8281 taaatggtct agctatgcac aaagccgtac aaacaaacct gttaaaggta tgttgactgg
     8341 acctgttacc atcctcaact ggtcattccc acgtgaagac atctctatca aggattcaac
     8401 tcttcaaatc gcccttgcta tcaaggatga agtgcttgac cttgaagctg ctggtgtgaa
     8461 aatcatccaa attgatgagg ctgctcttcg tgaaaaattg ccactccgcc gtagcgactg
     8521 gtacgaagac taccttgact gggctattcc tgccttccgc ttggtacact caacagtagc
     8581 gccagacaca caaatccaca ctcacatgtg ttactcagaa tttacagata tcatcccagc
     8641 tatcgataac atggatgcag acgttatttc ctttgaagct agccgttcaa accttgaaat
     8701 cttggacgaa ctcaaagcga aaaacttcca aacagaagtg ggacctgggg tttacgatat
     8761 ccactcacct cgtgtaccaa atgaaggcga aatcgacaac acaatcgaag ccatccttgc
     8821 taaagtgcca agcaagaaag tttggatcaa ccctgactgt ggtttgaaaa cacgtggtat
     8881 cccagaaaca aaagaaagct tgatccgcct tgttgaagca gctaaagctg cgcgtgagaa
     8941 attgtaagat tggatttctc tccatacagt ggttgttcaa aaagaaatca actagaaaag
     9001 gttattacat atgtcacgcc aaacaccgtc actctcattt gaagtgttcc ctccaaaccc
     9061 agccgtgggt aatgataaca ttatttcagc cttgcaggat atgcaggagt tggttcccca
     9121 ttttatcagt gtaactgcca gcaataataa atttaatatc aaggaaacga cggttcgttt
     9181 ggctgacttt atccaaaatg atttggcgat tccgaccatt gcccacttgc cagctattta
     9241 tctaactaag gacaaggttg ctgaaactat tgctgacttg gacaaagttg gggtgcagaa
     9301 aatcttggct cttcgtgggg atattattcc agacgtggaa ccacaaaagg atttccgcta
     9361 tgcaaccgac ttgattgagt ttatcaagga acgagcccct cactttgata ttattggagc
     9421 ttgctatcca gaagggcatc cagattcgcc aaatcagatt tcagatattc aaaatcttaa
     9481 gaagaaagtg gatgcaggct gttcgagcct cgtaactcag ctcttctttg acaatgagcg
     9541 cttctatgat tttcaggaca agtgtatctt ggctgggatt gatgttccca ttcatgcagg
     9601 aattatgcca attctgaatc gaaatcaggc tctccgactc ttgaagactt gtgagaatat
     9661 ccatcttcca cgcaaattta aagccatctt agacaagtat gagcatgacc ctgagtcgct
     9721 cagagcagca ggacttgcct atgcagtgga ccaaatcgtg gacttggtaa ctcaggatgt
     9781 tgccggtgtg catctctata cgatgaataa tgctgataca gcaaaataca tccatcaagc
     9841 aacccatgcc ttgtttaatc accagtctct aggataataa aagcaaacca ttcttctcag
     9901 gtgaggggaa tggttccttt ttaatggtaa agacctcact ttttaagaaa aatatgataa
     9961 aataaattct gtacgtactt gatacaaaga tgaaggtatt aaataataca atgcatttaa
    10021 actgtaagat ttaacgacac gagtgagtca aatcgatgtt atttgacgag agagttagta
    10081 aagcatttag ctaatcgccc gatagcgatt agcgtgaaag ctttagatac tttagtatta
    10141 aacctttcta atgatgtatc ttgaaaactg aacttgccta caactctgtg gaaaaagata
    10201 aatctcccta ggagtagtcg ctccgtcgtt cgatttccta tcttcctttg agttgcttga
    10261 cggcttggta tcttgatgac ttgaattttg gtctcatttc ttaggaaaaa agataagctt
    10321 cctagcactc gtcgagtgtg acgtcacctt cctatttttc tacagaaatg ttcggcaagc
    10381 cgaaccgtcc aaaatatctt gtgtaattga acacggccga aaagctgtgt aaaaaagata
    10441 aactgtcttg tcttcatcga agacttcgtc agtttcctat ttttactttg cttttgacgt
    10501 ccttggtatc ttgatctttg taggcaaggc gtataatttc atcaatccaa aggggattaa
    10561 aatggcaaaa caagtgtttc aaacgacttt tgcgggtcgt gagttaattg tagaaactgg
    10621 tcaggttgct aagcaagcaa atggctctgt tgtcgtacgt tacggtgagt caactgtctt
    10681 gactgctgcc gttatgtcta agaaaatggc aactggggat ttcttcccac tccaagtcaa
    10741 ctacgaagaa aaaatgtatg cggctgggaa gtttcctggt ggctttatga aacgtgaagg
    10801 acgtccttca acagatgcga ccttgacagc gcgtttgatt gaccgtccga ttcgtcctat
    10861 gtttgcggaa ggtttccgta atgaagtcca agtcatcaat acagtgcttt cttatgatga
    10921 aaatgcatct gcaccaatgg ctgctatgtt tggttcatct ttggcactgt ctatttcaga
    10981 tattccattt gacggaccaa ttgctggggt acaagtggga tatgtagatg gccaaatcat
    11041 catcaaccca agtcaagaac aagcagagca atctcttctt gaattgacag tagctggaac
    11101 caagcacgct atcaacatgg tagagtctgg tgccaaagaa ttgtcagaag aaatcatgtt
    11161 ggaagcgctt cttaaagggc acgaagctgt caaagaattg attgccttcc aagaagaaat
    11221 cgttgctgct gtcggtaaag aaaaagcaga agtggaattg cttcacgtgg atgctgaatt
    11281 gcaagctgaa atcattgcag cctacaacag tgacctccaa aaggcagttc aagtagaaga
    11341 gaaattggcc cgtgaagctg caactcaagc agtgaaagac caagtgactg ccgtttacga
    11401 agaaaaatat gcgaaccacg aagaatttga ccgtattatg cgtgatgtgg ctgaaatctt
    11461 ggaacaaatg gaacacgcag aagtgcgacg tttaattaca gaagacaagg tgcgtcctga
    11521 tggtcgtaag gtcgatgaaa tccgtccttt ggatgcggtt gttgacttcc ttcctcgtgt
    11581 acatggttca ggtctcttta ctcgtgggca aactcaagct ctttcagtct tgaccttggc
    11641 tccgatggga gaaactcaaa tcattgatgg tttggatcca gagtacaaga aacgctttat
    11701 gcaccactat aacttccctc aatattctgt aggggaaaca ggtcgttacg gtgcgccagg
    11761 tcgtcgtgaa atcggtcacg gtgcccttgg tgagcgtgct cttgctcaag tcttgccaag
    11821 cttggaagaa ttcccatacg ctatccgtct agtagcagaa gttttggaat caaacggttc
    11881 ttcatctcaa gcttctatct gtgcgggaac tcttgccctt atgactggtg gtgtgccaat
    11941 caaggcgcca gtagctggta ttgctatggg acttatctca gatggaaata actacacagt
    12001 attgacagat atccaaggtt tggaagatca ctttggagat atggacttca aggttgcagg
    12061 tactcgtgat gggattacag cccttcaaat ggatatcaag attcaaggga ttactgcaga
    12121 aatcttgacg gaggctcttg ctcaagccaa gaaagcgcgt tttgaaatcc ttgatgtcat
    12181 tgaagcaacc attccagaag ttcgtccaga attggctcca actgctccga aaattgatac
    12241 gatcaagatt gatgtggaca agattaagat tgtcatcggt aagggtggag aaaccatcga
    12301 caagattatc gctgaaacag gtgttaagat tgatatagac gaagaaggaa atgtgtctat
    12361 ctactctagt gaccaagatg ctattaaccg tgccaaagaa attattgctg gtttggttcg
    12421 tgaagccaaa gtggatgaag tttaccgtgc taaagtcgtt cgtatcgaga aatttggtgc
    12481 ctttgttaac ctctttgata agacagatgc ccttgttcat atctctgaga tggcttggac
    12541 tcgtaccaat cgtgtagagg atttggtaga aatcggggat gaagttgatg ttaaggttat
    12601 caagattgat gaaaaaggcc gtatcgatgc ctctatgaag gctcttctac ctcgtccgcc
    12661 aaaacctgag catgatgaaa aaggtgaaaa gtctgagcgc cctcaccgcc cacgtcatca
    12721 caaggatcac aaacctaaga aagaatttac agaaacacca aaagattcag agtaagaaaa
    12781 ggagaaatgt atggggtggt ggcacgaaac cattgatatc gtaaaagaaa atgatccagc
    12841 ggcccgcacc actttggagg ttttgctgac ttatccaggt gtcaaggcct tggcggccca
    12901 ccgtctctcg cattttctct ggaagtacga cttcaaactc ttagctcgta tgcacagtca
    12961 gttttggcgc ttttggactc agattgagat tcatccagga gcccagattg attcaggtgt
    13021 ctttattgac catggttctg gtctggtgat tggagagaca gcgattgttg aaaaaggcgt
    13081 tcttctctat cacggagtga ctctcggggg aacagggaaa gactgtggca aacgccatcc
    13141 gactgtacga aagggagccc tcatatcagc ccatgcccaa gttatcgggc ctgtggaaat
    13201 tggtgaaaat gccaaggtcg gtgctgcagc agttgtcgta gcagatgtac ctagtgatgt
    13261 gacggttgtc ggtattccgg ccaagattgt ccgtcttcat ggtaagaaag atgagcctgt
    13321 tattcacgaa gtcgaagaaa aacgggagta ttatgtcaat aaactcgagc aggctaaaga
    13381 tgccagtcac agatcgtctg gtttgtagag gatacctata tgattcaagc aagaaacaag
    13441 ttaagccaag aggagctatc tgaggcgaaa aaagtaatta actgttgcca aaactatgac
    13501 ggtacctatc gcgatcccta tctctctaac atgcttaatt ttgacccaaa catgcccgcc
    13561 tttttccttt attatgaaaa aggcaaactt gttggtttat taactgtcta tgcagatgac
    13621 caagatgtag aagtgacgat attggttcat ccaggtcatc gccgtcaggg gattgcgcgt
    13681 gcattgttta ctagttttga gagagaaaca gcttctttcc ccattcggtc agtcactttt
    13741 cagacagaac gtattttttt agagaatcat cctgactttg ccagtaactg gggactaatc
    13801 gaggatgaag agacagaaac ttggttaggt aaggatagaa gaccttatcc gttagcaaat
    13861 gtttcccatc ttgaagtttt gttagcagat agttcgtatc aggatcaaat tagtcaatta
    13921 aaatttcagg cattttcaga ggaacatgaa tcgagagaag ttgtggatag atatgtcgct
    13981 gaagctctga aggatccaga aagtcgctta tatattttgt taaaagacgg tcaggttatt
    14041 gggacttgca cggtagattt atcgactaat acgaattact tctacggttt agcaatatcg
    14101 gaacctgaac gtgggaaagg ctatggaagc tacttagcaa aatccctcgt caaccaacta
    14161 attgagcaaa atgacaagga atttcagatt gcagtggaag atagcaatgt aggtgccaaa
    14221 cgtttgtatg aaaaaattgg ctttgtcaaa cagacacagg tggtttatct gaatgagaaa
    14281 ggagcaaggg attccgaagt gtagagacat tcggactgaa atttacttga gctcttagtt
    14341 tagtgatgaa actaacagtt cttggatttt agctttcctg attatgattt atgattaaaa
    14401 tctatgacac catgtctcgt gatttgcgag aatttgtccc gattgaggac ggcaagatca
    14461 agatgtatgt ttgtgggcca accgtgtaca actatattca cgtgggaaac gcccgttcga
    14521 cggtagcttt tgatacgatt cgtcgctatt ttgagtaccg tgggtataag gttgcctata
    14581 tttccaattt tacagatgtg gatgataaga ttatcaaccg tgccagggaa gaaggcatca
    14641 cacctcagga ggttgcggat aagtacatcg ctgcctttcg tgaggatgtg acggccttag
    14701 gcgtgaaacc tgcgactcgc catccgcgtg tagtggagtt tatggcagac atcatccgtt
    14761 ttgtggaaga cttgatcgag aaaggctttg cctatgagag tcaaggggat gtctatttcc
    14821 gtgtagaaaa atcccacaac tatgctaaat tggctaataa aaccttggaa gatttggagc
    14881 taggtgcttc aggtcgtacc gatgaagaaa cggctcgtaa ggaaaatcct gtagattttg
    14941 ctctatggaa atcttccaaa ccaggtgaga tttcttggga tagtccttgg ggacctggac
    15001 gtccgggttg gcatattgag tgttcagtca tgtcgacaga gattttaggt gataccattg
    15061 atatccacgg tggtggagct gacctagagt ttccacacca caccaatgaa attgcccaat
    15121 ctgaagctaa aacaggcaag gcttttgcta actactggat gcacaatggc tttgtcaata
    15181 tcgacaatgt caaaatgtct aagtccttgg gtaacttcat tacggtacac gatgccctta
    15241 aaactcttga tgggcaagta cttcgtttct tctttgcgac ccagcactac cgtaagccta
    15301 tcaacttcac ggaaaaggca gttcgcgatg ccgagaccaa tctcaagtat ctgaagaata
    15361 cttacgaaca accatttaca ggaaacgtgg atgcccaaga gttacaaaac tttaaagata
    15421 agtttgtagc agctatggat gaagatttta atgctgccaa cggtatcaca gttgtctttg
    15481 aaatggccaa atggatcaac tcagggaact atgatgcaag tgtcaagcaa gctcttgcag
    15541 atatgttaga aatttttgga attgtctttg ttgaggaagt tttggatgca gagattgaag
    15601 acttgattca aaaacgccaa gaggcgcgtg ccaatcgaga ctttgagaca gcagaccaaa
    15661 tccgtgacca attggttact caaggaatta agctccttga taccaaggat ggagtgaggt
    15721 ggacacgtga ttgatgtcaa tctcattaac gggattgcgc tagcctttga gggggatgcg
    15781 gtgtattcta tgtatattcg ccgtcacctc atcctcaaag gtatgaccaa acccaataaa
    15841 ctccatcaag aagcaaccaa gtatgtgtca gccaaggctc aggctcgcct gattgctctc
    15901 atgttggagg agcaggtcct aacggaaaaa gaagaagaaa tctacaaacg tggccgcaat
    15961 accaatagcc acacaaaggc taaaaatgca gatgtcgtga cttatcgtat gtccacggga
    16021 tttgaagcgg ttatgggcta tctccatatg actgagaatc tggaacgtct tgagagtttg
    16081 gtttcatggt gcatccaaaa agtggagggc tagaacatga gggaaaaaga actacaagac
    16141 tggtttcctg aggctcggat ttcagaccaa ccagtagaga aagagggcta tctcacgctc
    16201 cctttagctt ctcagcagtg gattttgctg gaggaagctg ggctcagcga gcgtgaaaag
    16261 cagttggttg cccttttgac ccagcaggag caggctcgtt cgctaaaccc ttggtattcc
    16321 tatctggttg aaggcaaggg acaggcaccg caagttttta aaaagattca gttggtttat
    16381 tgccatcttt cttattttca gcaggaaaat ctggcttctt ggctagatat gatgcggact
    16441 ctttttccga attgtcagac agtgctacag gtcggagctc aggattatgt tttcgtgctt
    16501 caacaagaca aatacacttc tgtaagagat attttaagtg atacgattga agcggttgag
    16561 tatgactttg gacttcgtct ttctatcatg ttgggtcagg tttggtctca gacaggacat
    16621 caagccctat cagacttaat caaagctgag cgggatttgt tcaagacatg gtggcgtcag
    16681 ggtcaccaag gtgttcatac tttttctcag ctctatcttt ggagtatggg agaaagactc
    16741 gtggacttga agccaattaa agaatgtcta caccagatga ttttggatca agatcagatt
    16801 caggaaatca ttctctctct ttgggaaaat agtgctgttc tcactaaaac agcccagcaa
    16861 ctctatctgc accgcaattc tctccaatac aagattgata aatgggaaga gttgacaggg
    16921 cttcagttga aagagttgac cgacctgacc ttgtgttatc aattgatttt aggttctttg
    16981 tcaactatag ttggtttgta aagaagttaa tatttggaga agaggattgc catcttctcc
    17041 attt
//
LOCUS       DAVLYR010000022        16921 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLYR010000022 DAVLYR010000000
VERSION     DAVLYR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.022"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            41..457
                     /locus_tag="VYO63_000653"
     CDS             41..457
                     /locus_tag="VYO63_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV1671620.1"
                     /translation="MLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYINQEFKSYIDH
                     MLNKEAKIIGFSAGPLLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDK
                     ANKDRAEELDNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            739..987
                     /locus_tag="VYO63_000654"
     CDS             739..987
                     /locus_tag="VYO63_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEV1671621.1"
                     /translation="MCKPISIELCDDEVHSLHEWIDGRDAIDSILTYSENQQYTYGVE
                     AGKILRKIHTIPATEVCEDWEIFFNLKIDDKISNEMIW"
     gene            1163..1416
                     /locus_tag="VYO63_000655"
                     /pseudo
     CDS             1163..1416
                     /locus_tag="VYO63_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1544..2773
                     /locus_tag="VYO63_000656"
     CDS             1544..2773
                     /locus_tag="VYO63_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1671622.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLMMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2793..5489
                     /gene="ppc"
                     /locus_tag="VYO63_000657"
     CDS             2793..5489
                     /gene="ppc"
                     /locus_tag="VYO63_000657"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1671623.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5572..5789
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5902..6906
                     /locus_tag="VYO63_000658"
     CDS             5902..6906
                     /locus_tag="VYO63_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1671624.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPEFVLKEAGQVIE
                     "
     gene            6975..7841
                     /locus_tag="VYO63_000659"
     CDS             6975..7841
                     /locus_tag="VYO63_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671625.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7838..8596
                     /locus_tag="VYO63_000660"
     CDS             7838..8596
                     /locus_tag="VYO63_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671626.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            8686..10446
                     /gene="dnaG"
                     /locus_tag="VYO63_000661"
     CDS             8686..10446
                     /gene="dnaG"
                     /locus_tag="VYO63_000661"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1671627.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPYQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRVGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VKEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10449..11558
                     /gene="rpoD"
                     /locus_tag="VYO63_000662"
     CDS             10449..11558
                     /gene="rpoD"
                     /locus_tag="VYO63_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1671628.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11572..11901
                     /locus_tag="VYO63_000663"
     CDS             11572..11901
                     /locus_tag="VYO63_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1671629.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12016..13059
                     /gene="cpoA"
                     /locus_tag="VYO63_000664"
     CDS             12016..13059
                     /gene="cpoA"
                     /locus_tag="VYO63_000664"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1671630.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13075..14400
                     /locus_tag="VYO63_000665"
     CDS             13075..14400
                     /locus_tag="VYO63_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1671631.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14431..14607
                     /locus_tag="VYO63_000666"
     CDS             14431..14607
                     /locus_tag="VYO63_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1671632.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIVPVATDY"
     gene            14666..14800
                     /locus_tag="VYO63_000667"
     CDS             14666..14800
                     /locus_tag="VYO63_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1671633.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14864..16174
                     /gene="obgE"
                     /locus_tag="VYO63_000668"
     CDS             14864..16174
                     /gene="obgE"
                     /locus_tag="VYO63_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1671634.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16184..16342
                     /locus_tag="VYO63_000669"
     CDS             16184..16342
                     /locus_tag="VYO63_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671635.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(16394..16740)
                     /locus_tag="VYO63_000670"
                     /pseudo
     CDS             complement(16394..16740)
                     /locus_tag="VYO63_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5223 a         2904 c         3773 g         5021 t
ORIGIN      
        1 agtttttcaa agtcaatttc ctaatctaaa cattgattac ttgctcctgg acactgagca
       61 gattgatctt gatagctatc tagatgctga cataattatc atcggtggtg gaaatacgga
      121 aaaatatatt gctacttata ttaatcagga gttcaaaagt tatatcgatc atatgcttaa
      181 taaagaggca aaaattatag gattttctgc aggaccccta ctattaggag aaaaagtcta
      241 tgtctcacct aatgataatt cagatcatca gataaagata aaaaatggat taggactctt
      301 tagtcagttt ttaattagtg tccattatga ttcctggaat gataaagcaa ataaggatag
      361 agctgaagaa ctcgataatg ttcccataat tccactaaat gatcattctt gtcttgtatt
      421 ggataaactt ggaaacatca tagagaaaat tgactaactt tcataatgta aaaatggatc
      481 taggaaataa aagtgtagac gaactggata ggttactagt gaaaactgca aaaagtgttg
      541 taaagtgaat cggagtgtgc ttgatggatt ttagtagtaa gatagccata aataaaggct
      601 ggtcagatga taaaaaatat tgtgtgacag atcaaaatca gtaaaaatat ttcttgcgtg
      661 tttctgataa gtcgaagtta gattataaaa aaatttgaat ttgatatgat ggagaaagta
      721 gcttctcttg gagttccgat gtgtaaacca attagcattg aactctgcga tgacgaagta
      781 cattctttac acgaatggat agacggaaga gatgcaatag atagcatttt aacttattca
      841 gaaaaccaac aatacacata tggtgtagaa gcaggaaaga tacttagaaa gattcataca
      901 attcctgcta cagaagtttg tgaagattgg gaaatctttt ttaatctaaa aattgatgat
      961 aaaatctcca acgaaatgat atggtaagtc cgaaacgctc agtgtgtaag cttgttgcta
     1021 aaagcgtaaa aaccttggaa cgaaaggaat aatagacttc ctgcgaaaca aaaatacaat
     1081 acaataaaac tatggatgat gaagcaagta aacaattaag cgatagccgt ttcaagagcc
     1141 ttgtaggtgt tcagcgcacg agatgttagc tgtgttaaaa acagcttatc aacgcaacgt
     1201 aaacgcgcaa aaggtggacg aaaaaccaaa ttaagcctag acgatctcct catggtaact
     1261 attcaataca tgcgagaata gcgcacttat gaacaaattg cggctgattt tggcattcac
     1321 gaaagcaact taatccgtcg gagccaatgg gttgaagtaa ctcttgttca aaatggtttt
     1381 acgatttcaa attctgccct aattatgaaa aacagtaaaa ttcgaaggat tgtaaggtaa
     1441 gagttttttt ctttctgaaa aaatggtata atagcaatca aaactagaaa ataaaacgga
     1501 atttggaaca gatttgtctg tatcctagta gagtggtgat gctatgaaga ttagtaagag
     1561 gcacttatta aattattcca tcttgattcc ctacttgctt ttatctattt tgggcttgat
     1621 tgtggtctat tcgaccacca gtgctatttt aattgaagaa ggcaagagcg ccttgcagtt
     1681 ggttcgaaac caaggaatct tttggattgt tagtttgata ctgattgcct taatttataa
     1741 attgagacta gattttttga gaaatgagcg actaatcatt ttagttatat taatagaaat
     1801 gcttttattg ttcttggctc gttttattgg tatttccgta aacggggcat acggttggat
     1861 ttcggttgca ggaataacta ttcagccagc tgagtactta aaaatcatta ttatttggta
     1921 tttagctcac cgattctcca aacagcaaga agaaatagct acttatgatt ttcaagtttt
     1981 gactcaaaat caatggcttc cccgtgcttt taatgattgg cgattcgttc tcctagttct
     2041 gattggaagt ttgggaattt tccctgattt aggaaatgcg actattttag tcttggtttc
     2101 cttgatgatg tatacagtta gtggaatcgc ttatcgctgg ttttcaacca ttctggcgct
     2161 cgtatctgcc gcttctgtct ttgtcttgac cactatcagc ctaatcggtg ttgagacctt
     2221 ttcaaaaatt ccagtatttg gctatgtagc caagcgcttt agtgcctttt ttaatccttt
     2281 tgccgatcgt gctgatgcag gtcaccagtt agctaattct tattttgcca tggtcaatgg
     2341 cggttggttt ggtctaggtc ttggaaactc gattgaaaaa cgaggttatt tgccagaagc
     2401 tcatacagac tttgtctttt ctatcgtgat tgaagaattt ggctttgttg gtgccagtct
     2461 tattttagct ctcttgtttt tcatgatttt gcggattatc ttggtcggta tccgagcgga
     2521 gaatcctttc aatgccatgg ttgcactcgg tgtcggaggg atgatgttgg ttcaggtatt
     2581 tgtcaatatc ggagggattt cgggcttgat tccatctaca ggagtgactt tccccttctt
     2641 atcccagggt ggaaatagtc ttctagtctt atcagtggca gtagcctttg tcttaaatat
     2701 tgatgccagt gaaaaacgcg ctaaattgta ccgagaattg gaaaatcaac caatgaacct
     2761 tctgttgaag taggataaag aaaggatagt ttatgtctct tcaaaaatta gaaaattata
     2821 gtaataaaag tgttgtgcaa gaagaagtct tgattctaac agaattactg gaagatatta
     2881 ctaaaaatat gcttgcccca gagacctttg aaaaaataat acagttgaaa gaattatcaa
     2941 cgcaggaaga ttatcaaggt ctaaaccgtc tagtgactag cttatcaaat gatgaaatgg
     3001 tctatatttc acgctatttc tctatcttgc ctcttttgat taatatttca gaggatgtgg
     3061 atttagctta tgaaatcaat catcaaaata atattgatca ggactattta ggtaaattat
     3121 ctacaacgat taaattggta gcagaaaagg aaaatgccgt tgagatccta gaacacttga
     3181 atgttgtccc tgttttgaca gcccatccaa cacaagtgca acgcaaaagt atgttggatt
     3241 taacaaatca tattcatagt cttttgcgta aataccgtga tgttaagttg gggttgatca
     3301 ataaagataa atggtacaat gatttgcgtc gttacatcga aattatcatg cagacagaca
     3361 tgattcgtga gaaaaaatta aaagtgacta acgaaatcac gaatgctatg gaatattata
     3421 acagctcctt tttgaaagct gtacctcatt tgacgacgga gtataagcgc ttagcgcaag
     3481 cgcatggtct gaatttaaaa caggctaaac caatcaccat gggtatgtgg ataggtggtg
     3541 accgtgatgg aaatccattt gttacagcaa agaccttgaa gcagtctgca ctcactcagt
     3601 gtgaagtcat catgaactac tatgataaaa agatttacca actttatcgt gaattttctc
     3661 tttcaactag cattgtcaac gtcagcaagc aagtcagaga aatggctcgt caatccaagg
     3721 ataactcgat ttaccgcgaa aaagagcttt accgtcgtgc cttgtttgat attcaatcaa
     3781 aaattcaggc aactaaaacc tatctgattg aggatgaaga agttgggact cgttatgaaa
     3841 ccgccaatga tttctacaag gatttgattg ccattcgaga ttctctacta gaaaataagg
     3901 gcgagtcctt gatttcaggt gattttgtgg aattattgca ggcagtagag atatttggtt
     3961 tttacttagc atcaattgat atgcgacaag actctagcgt ctatgaagcc tgtgtggcag
     4021 aactcttgaa atcagcagga attcattctc gttatagcga gttgagcgaa gaagaaaagt
     4081 gtgaccttct cttgaaagaa ttagaagaag atccccgaat tctttctgcg actcacgcag
     4141 aaaaatcaga attattagca aaagaactag ctatttttaa gacggctcgt gttttgaaag
     4201 ataagttggg agatgatgtc atccgtcaga ccatcatttc acatgcaacc agcctttctg
     4261 atatgctaga attagctatt ctgttaaaag aagtaggact ggtggatacg gaaagggcgc
     4321 gtgttcagat tgttcccctt tttgaaacaa ttgaagactt ggatcattca gaggaaacaa
     4381 tgagaaaata tctttctctt agccttgcca aaaaatggat tgactcacga aataactatc
     4441 aagaaatcat gcttggctac tctgacagta ataaagatgg cggttacttg tcatcatgtt
     4501 ggaccctcta caaggctcaa caacaattga ctgctattgg agatgaattt ggcgttaagg
     4561 ttaccttctt ccatggtcgt ggtggtactg tcggtcgtgg tggtgggcca acctatgaag
     4621 ccattacatc tcaaccgctc aagtctatca aggatcgtat ccgcttgacg gagcagggtg
     4681 aagtaattgg gaataaatac ggtaacaaag acgccgctta ctataacctt gaaatgctag
     4741 tatcggcagc tattaaccgt atgattactc agaagaagag cgataccaat accccaaatc
     4801 gttatgaagc cattatggat caagtagtgg accgtagtta cgatatctac cgtgatttgg
     4861 tctttggtaa tgagcatttc tatgattatt tcttcgagtc aagtccaatc aaggctattt
     4921 caagttttaa tattggttct cgtccagccg ctcgtaagac tattactgaa atcggtggat
     4981 tgcgtgctat cccttgggta ttctcatggt cacagagtcg tgttatgttc cctggatggt
     5041 acggggttgg ttcaagcttc aaggaattta tcaataaaaa tccagagaat attgctatct
     5101 tacgagatat gtaccaaaat tggcctttct tccaatcgct tctttcaaat gttgatatgg
     5161 ttttgtcaaa atcaaatatg aatattgctt ttgaatatgc taaactttgt gaagacgagc
     5221 aagttaaggc catctatgag actattttaa atgaatggca agttactaag aacgttatct
     5281 tggctattga aggacatgac gaactcttag ctgacaatcc atatctaaaa gctagtctgg
     5341 attaccgtat gccttacttt aatattctca actatattca gttagagttg attaaacgcc
     5401 aacgtcgtgg agaattgtcc agtgatcaag aacgattgat tcatatcacc atcaacggaa
     5461 ttgcgacagg attgcgtaat tcaggttgat aattttcaag agtgaatgct aaaagtgaat
     5521 atcaaaaaaa ttctaataga ctattgacaa gtagtttaaa aatgatataa tttaaccatt
     5581 cagaaaagta atcatacaaa ctttttagag agtctgtggt agctgaaaac agataagtgg
     5641 caatgatgaa aattgggctg aatgctattt agaatttgaa attataaaaa ttcggtaagc
     5701 acaccttaca gtgcatctcg ttattgcgag actgagcgat agggaaattc cctataattg
     5761 aggtggtacc gcgcatcgac gtcctcacac aagttttttg tgtgaggatt tttttgatgg
     5821 aggttagtat agaaagaaaa cgatggcgtc gcttgtttag ataagtgaaa tatgttaaag
     5881 gaaataaaaa ggagaaacag aatgaaaaat aaacgtttaa ttggaattat tgctgcatta
     5941 gcagtcttag tagcaggaag cttgatttat tcttcaatga ataaatcaga agctcagaat
     6001 aataaggatg agaagaaaat aaccaagatt ggtgtgcttc aatttgtgag ccatccatcc
     6061 cttgatttga tttataaagg gatccaagat ggacttgcag aagaaggata taaagatgat
     6121 caagttaaaa ttgattttat gaactcagaa ggtgaccaaa gtaaggttgc gacaatgagt
     6181 aaacaattgg ttgcaaatgg gaatgacctt gtggttggta tcgcaacacc agcagcccaa
     6241 gggttggcta gtgcaacaaa agacctaccg gttatcatgg ccgctattac agacccaatt
     6301 ggtgctaact tggttaaaga tttgaaaaaa ccaggtggca acattacagg ggtatctgac
     6361 cacaatccag ctcaacaaca agttgaactc atcaaggctc tgacaccgaa tgtgaaaaca
     6421 atcggagctc tttactcaag tagcgaagac aattcaaaaa cacaggtcga agaatttaag
     6481 gcttatgctg aaaaagcagg tctgacagtg gaaacatttg cagttccttc aacaaatgaa
     6541 attgcctcaa ctgtcactgt tatgactagc aaggtagatg ctatttgggt tccaattgat
     6601 aacaccattg catcaggatt tccaacggtt gtctctagca atcaaagttc taagaaacca
     6661 atttatccca gtgcgacagc tatggtagaa gtaggtggtt tggcatcagt tgtaattgac
     6721 caacatgacc ttggtgtggc aacaggtaaa atgattgtgc aagtcttgaa aggtgcaaaa
     6781 ccagccgata ccccagtcaa tgtcttttca actggtaagt cagtcatcaa taaaaaaata
     6841 gcacaagaac taggtattac tattcctgag tttgttctca aagaagcagg acaagtcatc
     6901 gaataaataa ggtagggagg aggagactct tcccattttt taaaaagatg aatatagaaa
     6961 ggattaaata aaatatgatt gtttccatta tttctcaagg atttgtctgg gctattctag
     7021 gtctgggaat ctttatgaca tttaggattt taaactttcc agatatgacg acagaaggtt
     7081 ccttccctct tgggggagct gttgctgtca ctttgataac caaaggcgtg aacccatttt
     7141 tagcgacact tgttgctgta ggagcaggtt gtttggctgg aatggcagca ggccttcttt
     7201 atacaaaagg gaagatccca accttgctct cagggatttt ggtgatgact tcttgtcact
     7261 caatcatgct cttgattatg ggacgtgcaa atttaggcct gcttggaacc aagcaaattc
     7321 aggatgtttt gccttttgat tcggatttga atcaactctt gacaggtctc atctttgtga
     7381 gtattgttat tgctctcatg ctctttttct tggacactaa actcggacaa gcctatattg
     7441 ctacagggga taatcctgat atggctagaa gtttcgggat tcatactgga cacatggagc
     7501 tcatgggctt ggtcttatca aatggtgtga ttgcccttgc aggtgccctc attgctcagc
     7561 aagaaggtta tgccgatgtg tctcgaggga tcggggttat cgttgtgggg cttgcaagtt
     7621 tgattattgg agaagttatt ttcaagagtt tgagcttggc agagcgtttg gttactatcg
     7681 ttgtaggttc tatcgcttat caatttttag tgtgggcagt tatcgcactt ggctttaata
     7741 caagttacct tcgtttatac agtgccttga ttttagcagt ctgcctcatg attccaacat
     7801 ttaagcaaac aatcttgaaa ggagccaagt taagcaaatg acagcaattg tagaattaaa
     7861 aaatgcaacc aaaatcgtta aaaatggctt tgatgaagaa aagattattt taaatgatgt
     7921 ttccttagaa atttttgaac aggactttat cacgattttg ggtggaaatg gtgctggtaa
     7981 atcaaccctc tttaacacta ttgcaggaac catatcacta accagtggaa ctatccgtat
     8041 tttaggtgag gatgttacca agttttcacc agagaagcgc gctaagtatc tgtctcgagt
     8101 cttccaagat ccaaagatgg ggacagctcc tcgtatgaca gtggcagaga atctcctgat
     8161 tgccaagttt cgtggtgaaa agcgtggact atttccgaga cgtctggcta gctataagga
     8221 taagtttcag gcgaccattg aaaaagtggg aaacggtctt gagaaacact tgaatacacc
     8281 gattgagttc ttatctggtg gacaaagaca ggctttgagt ctcttgatgg caaccttgaa
     8341 gcgacctgaa ttactcttgt tagacgagca tactgctgcc ctggatccaa agactagtgt
     8401 tgctttgatg gaattgacag atgaatttgt taagaaagat caattgacag cccttatgat
     8461 tacccatcat atggaagatg ctctcaaata tggcaatcgc ttgattgtca tgaaagaagg
     8521 acgggttatc caagatttga aacaagaaga aaaagcaaaa atgaaaatct ctgattatta
     8581 tcaactcttt gaataaagta gggaaaatga gtgaaatggt ttactttttt tctgaaataa
     8641 agtatactat ataaagtaaa ctatgataac atggaggtat tgtgtatggt tgacaaacaa
     8701 gtcattgaag aaatcaaaaa caatgccaac attgtggaag tcataggaga tgtgatttct
     8761 ttacaaaagg caggacggaa ctatctaggg ctctgtcctt ttcatggtga aaaaacacct
     8821 tctttcaacg ttgtagagga caagcagttt taccactgtt ttggttgtgg tcgctcaggt
     8881 gatgtcttta aattcatcga ggagtaccaa ggggttacct ttatggaggc tgtccaaatc
     8941 ttaggtcagc gtgtcgggat tgaggttgaa aaaccgcttt atagtgaaca gaagccagcc
     9001 tcgccttacc aagctcttta tgatatgcac gaagatgcgg ctaaatttta ccatgctatt
     9061 ctcatgacaa cgactatggg cgaagaggcc agaaattacc tttatcagcg gggtttgaca
     9121 gatgaagtgc ttaaacattt ttggattggt ttagcacctc cagaacgaaa ctatctctat
     9181 caacgtttgt ctgatcagta tcgtgaagag gatttactgg attcaggcct gttttatctc
     9241 tcggatgcca atcaatttgt agacaccttt cacaatcgca ttatgtttcc cctgacaaat
     9301 gaccaaggaa aggtcattgc cttctcaggt cgtatctggc aaaaaacgga ttcacaaact
     9361 tctaagtata aaaacagccg atcgactgca atttttaaca aaagttacga attatatcat
     9421 atggataggg caaaaaaatc ttctggaaaa gctagtgaga tttacctgat ggaaggattc
     9481 atggatgtta ttgcagccta tcgggttgga atcgaaaatg ctgtggcgtc gatgggaacg
     9541 gccttgagtc gagagcatgt tgagcatctg aaaaggttaa ccaagaaatt ggttcttgtt
     9601 tacgatggag ataaggctgg gcaagccgcg atattgaaag cattggatga aattggtgat
     9661 atgcctgtgc aaatcgtcag catgcctgat aacttggatc ctgatgagta tctacaaaaa
     9721 aatggtccag aagacttggc ctatctatta acgaaaactc gtattagtcc gattgagttc
     9781 tacattcatc agtacaaacc tgaaaacagt gaaaatctgc aggctcagat tgagtttatt
     9841 gaaaaaatag ctcccttgat tgttaaagaa aagtccatcg ctgctcaaaa cagctatatt
     9901 catattttag ctgacagtct ggcgtccttt gattatgccc agattgagca gattgttaat
     9961 gagagtcgtc aggtgcaaag gcagaatcgc atggaaagaa tttccagacc gacgccaatc
    10021 accatgcctg tcaccaagca gttatcggct attatgaggg cagaagccca tctactctat
    10081 cggatgatgg aatcccctct tgttttgaac gattaccgtt tgcgagaaga ctttgcattt
    10141 gctacacctg aatttcaggt cttatatgac ttgcttggcc agtatggaaa tcttcctcca
    10201 gaagttttag cagagcagac agaggaagtt gaaagagctt ggtaccaagt tttagctcag
    10261 gatttgcctg ctgagatatc gccgcaggaa cttagtgaag tagagatgac tcgaaacaag
    10321 gctctcttga atcaggacaa tatgagaatc aaaaagaagg tgcaggaagc tagccatgta
    10381 ggagatacag atacagccct agaagaattg gaacgtttaa tttcccaaaa gagaagaatg
    10441 gagtaataat ggcaacaaaa caaaaagaag taacaacatt tgacgtacag gtagcagaat
    10501 ttatccgtaa tcataagcaa aaagggacag caacagatga tgaaatcaat gcaagtctgg
    10561 ttattccttt taccttggac gctgatggga ttgaagatct cttgcaacgg attcaggatg
    10621 caggcatttc tatcacagat aacgaaggaa atccaagtgc gcgtgttctt agcaatgaag
    10681 aagaaccaga actcagcgat gaggacttga ttgggtcaac ttctgctaag gtcaatgacc
    10741 ctgtccgtat gtacttgaaa gaaatagggg tcgttcctct cttgaccaat gaagaggaga
    10801 aagagttggc actggctgtt gaagctggcg atatcgaagc caaacaacgt cttgcggaag
    10861 ccaatcttcg tttggttgtt tccattgcca aacgctatgt cggtcgtggc atgcagttcc
    10921 ttgacttgat tcaagaagga aatatgggct tgatgaaggc ggttgacaag tttgactatt
    10981 ctaaagggtt caagttttca acttatgcaa cttggtggat tcgtcaggct atcactcgtg
    11041 ctattgcgga ccaagctcgt accatccgta tcccagttca catggttgaa actatcaata
    11101 aattggttcg tgaacagcgg aatctccttc aagaattggg gcaagatccg acaccagaac
    11161 agattgctga acgaatggat atgacacctg ataaggttcg tgaaatcttg aagattgccc
    11221 aagaaccagt atctcttgaa actcctatcg gtgaagagga cgatagccac cttggagact
    11281 ttatcgaaga tgaagtgatt gaaaatccag tggattatac gactcgtatc gtcttgcgtg
    11341 agcaattgga tgaaatctta gatactctta cagaccgtga agaaaatgtt ctgcgtctac
    11401 gttttggact agatgatgga aaaatgcgca cacttgaaga tgtggggaaa gtctttaacg
    11461 taactcgtga gcgtatccgt cagattgaag caaaggcttt gagaaaacta cgccaaccaa
    11521 gtcgcagcaa accgcttcgt gattttattg aagactaaga gtgaggaaaa tatggcttat
    11581 acagaagagc aaattgaaaa catcaaaaca cggattttaa cagccttgga agaagtcatc
    11641 gaccctgagt tgggaatcga tattgtcaat cttggtttga tctatgagat tcgttttgac
    11701 ggtgacacag ggcaaacaga gattgacatg actttgacaa ctatgggttg tcccttagca
    11761 gaccttttga cagaccagat ttatgatgct atgatagagg ttccagaagt aacggatact
    11821 gaggttaaat tagtctggta tccagcctgg acagttgaaa aaatgagtcg ctatgcccgc
    11881 attgccctag gcattaagta aacatataaa agcatgtgag agactgttgg aaaagcgagg
    11941 aaatttcccc tcttttcctc tagtctctcc tttcttttgc tgattttatt caaagaaaat
    12001 gatataatag tagttatgga gaaaaagaaa ttacgcatca atatgttgag ttcaagtgag
    12061 aaagtagcag gacagggagt ttcaggtgct taccgtgaat tagttcgtct tcttcaccgt
    12121 gctgccaagg accaattgat tgttacagaa aatcttccga tcgaggcaga tgtgactcac
    12181 tttcatacga ttgattttcc ctattattta tcaaccttcc aaaagaaacg ctcagggaga
    12241 aagattggct atgtgcattt cttgccagct acacttgagg gaagtttgaa aattccattt
    12301 ttcttaaagg gaattgtgaa acgctatgta ttttcttttt acaaccggat ggagcacttg
    12361 gttgtggtca atcctatgtt tattgaggat ttggtagcag ctggtattcc acgtgaaaaa
    12421 gtgacctata ttcctaactt tgtcaacaag gaaaaatggc atcctctacc acaagaagag
    12481 gtagtcagac tgcgcacaga tcttggtctt agtgacaatc agtttatcgt agtaggtgct
    12541 gggcaagttc agaaacgtaa agggattgat gactttatcc gtctggctga ggaattgcct
    12601 cagattacct ttatctgggc tggtggcttc tcttttggtg gtatgacaga tggttatgaa
    12661 cactataaga aaattatgga aaatccccct aaaaatttga tttttccagg cattgtatcg
    12721 ccagagcgga tgcgcgaatt gtatgctcta gcggatcttt tcttgttgcc tagttacaat
    12781 gagctctttc ctatgactat tttagaagct gcgagttgtg aggctcctat tatgttgcgt
    12841 gatttagatc tctataaggt gattttggag ggaaattatc gggcgacagc gggtagagaa
    12901 gagatgaaag aggctatttt ggaatatcaa gcaaatcctg ctgccttaaa agatctcaaa
    12961 gaaaaggcta agaatatttc cagagagtat tctgaagagc atctgttaca aatctggttg
    13021 gacttttatg agaaacaagc cgctttaggg agaaagtaaa aagtgaggta atctatgcga
    13081 attggtttat ttacagatac ctattttcct caggtttctg gtgttgcgac cagtattcga
    13141 accttgaaaa cagaacttga aaagcaggga catgctgttt ttatctttac gacgacagat
    13201 aaggatgtca atcgctacga agattggcaa attatccgca ttccaagtgt tcctttcttt
    13261 gcttttaagg atcgtcgctt tgcctaccga ggttttagca aggcacttga aattgctaaa
    13321 cagtatcagc tagatattat ccatactcag acagaatttt ctcttggcct gttggggatt
    13381 tggattgcgc gtgaattgaa aattccagtc atccatacct atcacaccca gtatgaagac
    13441 tatgtccatt atattgctaa ggggatgttg atccgaccga gtatggtcaa gtatctggtt
    13501 agaggtttcc tgcatgatgt ggatggggtt atttgcccta gtgagattgt ccgtgacttg
    13561 ctatctgatt ataaggtcaa ggttgaaaaa cgggtcattc ctactgggat tgaattagcc
    13621 aagtttgagc gtccggaaat caagcaggaa aatttgaaag aactgcgtag taaactaggg
    13681 attcaagatg gtgaaaagac gttgcttagt ctttcgagaa tctcctatga aaaaaatatt
    13741 caagcagttt tagtagcctt tgctgatgtt ctgaaagagg aagacaaggt taaactggta
    13801 gtagctgggg atggccctta tctgaatgac ctcaaagagc aagcccagaa cctagagatt
    13861 caagactcag tcatctttac agggatgatt gctcctagtg agacggctct ttactataaa
    13921 gcggcggatt ttttcatttc ggcatcgaca agcgaaacgc aaggtttgac ctacttggaa
    13981 agcttagcca gtggaacacc tgtcattgct cacggaaatc cttatttgaa caacctcatc
    14041 agtgataaaa tgtttggaac cttgtactat ggagaacatg atttggctgg tgctattttg
    14101 gaagccctga ttgcaacacc agacatgaac gagcatacct tatcagagaa attgtatgag
    14161 atttcagctg agaactttgg gaaacgagtg catgagtttt atctggatgc cattatttca
    14221 aataacttcc agaaagattt ggctaaagat gatacggtca gtcagcgtat ctttaagaca
    14281 gttttgtatc ttcagcaaca ggtggttgct gtacctgtaa aaggatctag acgcatgttg
    14341 aaggcttcaa aaacacagtt gatcagtatg agagactatt ggaaagacca tgaagaatag
    14401 aaagaggaac agctatgaaa aaaacaatta atgagaagcg gtcgtgataa aaagattgcg
    14461 ggtgtttgtg ctggggtggc ccattatctg gatatggatc cgactatcgt tcaagtcatt
    14521 tggggtgttc ttacttgctg ttacggagct ggaattgtag cttacattat tttatggatt
    14581 atcgtaccag tagcaactga ctattgaaaa ctagctcaat tcatgctaag ataatagaaa
    14641 atagaatgta acgaaggaga gaaaaatggc atttggagat aatggaaatc gtaaaaaaac
    14701 tatgtttgag aaaataacct tgtttatcgt gattatcatg ctagtagcaa gtttattggg
    14761 aatttttgca actgcaattg gtgccctcag taatctataa aatagattca agaaaattta
    14821 gtgactggga tttcccagcc cttttttaaa gtgagaagaa ataatgagta tgtttttaga
    14881 tacagctaag attaaggtca aggctggtaa tggtggcgat ggtatggttg cctttcgtcg
    14941 tgaaaaatat gtccctaatg gaggcccttg gggtggtgat ggtggtcgtg gaggcaatgt
    15001 ggtcttcgtt gtagacgaag gattacgtac cttgatggat ttccgctaca atcgtcattt
    15061 caaggctgat tctggtgaaa aagggatgac caaagggatg catggtcgtg gtgctgagga
    15121 ccttagagtt cgagtaccac aaggtacgac tgttcgtgat gcggagactg gcaaggtttt
    15181 aacagatttg attgaacatg ggcaagaatt tatcgttgcc cacggtggtc gtggtggacg
    15241 tggaaatatt cgtttcgcga caccaaaaaa tcctgcaccg gaaatctctg aaaatggaga
    15301 accaggtcag gaacgtgagt tacaattgga actaaaaatc ttggcagatg tcggtttagt
    15361 aggattccca tctgtaggga agtcaacact tttaagtgtt attacctcag ctaagcctaa
    15421 aattggtgcc taccacttta ccactattgt accaaattta ggtatggttc gcacccaatc
    15481 aggtgaatcc tttgcagtag ccgacttgcc aggtttgatt gaaggggcta gtcaaggtgt
    15541 tggtttggga actcagtttc tccgtcacat cgagcgtaca cgtgttatcc ttcacatcat
    15601 tgatatgtca gctagcgaag gccgtgatcc atatgaggat tacctagcta tcaataaaga
    15661 gctggagtct tacaatcttc gcctcatgga gcgtccacag attattgtag ctaataagat
    15721 ggatatgcct gagagtcagg aaaatcttga agaatttaag aaaaaattgt ctgaaaatta
    15781 tgatgaattt gaagagttac cagctatctt cccaatttct ggattgacca agcaaggtct
    15841 ggcaacactt ttagatgcta cagctgaatt gttagacaag acaccagaat ttttgctcta
    15901 cgacgagtcc gatatggaag aagaagctta ctatggattt gacgaagaag aaaaagcctt
    15961 tgaaattagt cgtgatgacg atgcgacatg ggtactttct ggtgaaaaac tcatgaaact
    16021 ctttaatatg accaactttg atcgtgatga atctgtcatg aaatttgccc gtcagcttcg
    16081 tggtatgggg gttgatgaag cccttcgtgc gcgtggagct aaagatgggg atttggtccg
    16141 cattggtaaa tttgagtttg aatttgtaga ctaggagact ggtatgggag ataaaccgat
    16201 atctttccga gatgcggatg gtaattttgt ttccgccgca gacgtttgga atgaaaagaa
    16261 attggaagaa ctatttaatc gtctcaatcc aaatcgtgcc ttgagattgg cacgaactaa
    16321 aaaggaaaat ccatctcagt aaagaagcta aaaaatcccg gctctttgtc aactgtagtg
    16381 ggttgaagaa aagctaagct cgagaaagga caaattttgt cctttctttt ttgatattca
    16441 gagcgatgaa aatccgtttt ttgaagtttt caaagtttcg aaaaccaaag gcattgcgct
    16501 tgataagttt gatgagatta ttggtcgctt ccagtttggc attagaatag tgtagttgaa
    16561 gggcattgac aatcttctct ttatctttga ggaaggtttt agaggatgaa cctgattcag
    16621 attgtcctca atgagtccga aaaatttgtc aggctcctta ttctgaaagt gaaaaagcaa
    16681 gagttgatag agattatagt ggtgtttcaa gtcttctgaa tagctcaaaa gtttatctat
    16741 agtagattga aactagaata gtacacctct gcttctaaaa cattgttaga aatcgatttg
    16801 actgtcctga atgatttgtc ctgttattat ttcattttac tataaatcca cgtttacgaa
    16861 tctctttcca cacttgttca atggggttca tctctggtgt gtatggagga ataaatgcaa
    16921 a
//
LOCUS       DAVLYR010000023        16880 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLYR010000023 DAVLYR010000000
VERSION     DAVLYR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.023"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            45..392
                     /locus_tag="VYO63_000671"
     CDS             45..392
                     /locus_tag="VYO63_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1671636.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEIEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(509..1642)
                     /locus_tag="VYO63_000672"
                     /pseudo
     CDS             complement(509..1642)
                     /locus_tag="VYO63_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1707..1988
                     /locus_tag="VYO63_000673"
     CDS             1707..1988
                     /locus_tag="VYO63_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1671637.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2120..3064
                     /gene="manA"
                     /locus_tag="VYO63_000674"
     CDS             2120..3064
                     /gene="manA"
                     /locus_tag="VYO63_000674"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1671638.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(3104..4439)
                     /locus_tag="VYO63_000675"
                     /pseudo
     CDS             complement(3104..4439)
                     /locus_tag="VYO63_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            4667..5416
                     /locus_tag="VYO63_000676"
     CDS             4667..5416
                     /locus_tag="VYO63_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1671639.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(5436..5891)
                     /locus_tag="VYO63_000677"
     CDS             complement(5436..5891)
                     /locus_tag="VYO63_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1671640.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5911..7086)
                     /locus_tag="VYO63_000678"
     CDS             complement(5911..7086)
                     /locus_tag="VYO63_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1671641.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(7145..7990)
                     /locus_tag="VYO63_000679"
     CDS             complement(7145..7990)
                     /locus_tag="VYO63_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1671642.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            8115..8672
                     /locus_tag="VYO63_000680"
     CDS             8115..8672
                     /locus_tag="VYO63_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1671643.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            8691..9158
                     /locus_tag="VYO63_000681"
     CDS             8691..9158
                     /locus_tag="VYO63_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1671644.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            9247..9876
                     /gene="upp"
                     /locus_tag="VYO63_000682"
     CDS             9247..9876
                     /gene="upp"
                     /locus_tag="VYO63_000682"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1671645.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            10050..10640
                     /gene="clpP"
                     /locus_tag="VYO63_000683"
     CDS             10050..10640
                     /gene="clpP"
                     /locus_tag="VYO63_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1671646.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            10719..10967
                     /locus_tag="VYO63_000684"
     CDS             10719..10967
                     /locus_tag="VYO63_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1671647.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            11068..12228
                     /locus_tag="VYO63_000685"
     CDS             11068..12228
                     /locus_tag="VYO63_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1671648.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGRDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            12496..13365
                     /locus_tag="VYO63_000686"
     CDS             12496..13365
                     /locus_tag="VYO63_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1671649.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGAISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFEMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            13369..14325
                     /locus_tag="VYO63_000687"
     CDS             13369..14325
                     /locus_tag="VYO63_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1671650.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGYAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            14325..15089
                     /locus_tag="VYO63_000688"
     CDS             14325..15089
                     /locus_tag="VYO63_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671651.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKDKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            15089..15799
                     /locus_tag="VYO63_000689"
     CDS             15089..15799
                     /locus_tag="VYO63_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671652.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            16158..16814
                     /locus_tag="VYO63_000690"
     CDS             16158..16814
                     /locus_tag="VYO63_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1671653.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
BASE COUNT         5157 a         3235 c         3598 g         4890 t
ORIGIN      
        1 tttaaaagat cggagcaatc cggtcttttt atggaggaga gtaaatgaat ttaaatcaat
       61 tagatattat cgtttcaaat gttccccaag tctgtgctga cttggagcat atcttggata
      121 aaaaggcaga ttatgctgat gatggttttg ctcagtttac gattggtagt cactgcctta
      181 tgttgtcaca aaatcatttg gttcctttgg aaaactttca gtcaggaatc attattcata
      241 tcgagattga ggatgtagac cagaactaca aacggttgaa cgagcttggt ataaaggttt
      301 tacacggacc aactgtaacc gattggggaa cagagtcctt attagttcaa ggacctgctg
      361 gtctagtgct tgatttttat cgtatgaaat aggtcgcgta gtcatttgac gttaacctta
      421 gctattttta aaatagaaat tgagaatatg aactattgaa agatcggagc aatccggtct
      481 ttttatataa agaaattgca tataatactc atctgctttc aaaaagcatt ctagtccatc
      541 tccgattaac gatggacttt atcacctcct tctccaatcc ttgtatgaca tcttggagtt
      601 gattcatgac atcttccaaa gttcgaaagg ctttattctt aaatccacgt ttacgaatct
      661 ctttccacac ttgttcaatg gggttcatct ctggtgtgta tggaggaata aatgcaaagc
      721 caatattagt cggaatcttt aaggtacttg atttatgcca tatagcattg tccataacga
      781 gtaaaaaata atcatatgga taagcttgtg aaagctcttc taaaaagacg ttcatccact
      841 cagtattaca tccaccagct cttaagaaaa atgattcgcc tgtataggca tcaacagctc
      901 cataacaata gcgaaattct cgtatatagt gactatggat atgtggacct actcctattg
      961 gagaccaaca agatcccagt ttactgattc taccgaaacc agcctcatct tggtacatca
     1021 agcgaacctt acgaaaacgt ctactggttt taaatcgctt tcttttcttc ttgaattgag
     1081 attttatttt tagacgcgac aatggtttga gcgtctgctt tcttaggatg ttctggacgt
     1141 ggcgtaatat ttcgccaacc atggcgcttc aacagttgat agaaggcatc acgtgtgtag
     1201 gaacgaccta actccttttt ataagcctga aataaggcat caattgtaac aaattctcct
     1261 gcctctgtag ccttcaaatg acgggcaaga aaggctttct cttcctcaac tgtcatatat
     1321 gcatggttac gaccaccacg tgtttcttga aggagagagt cgagtccgaa ctcctcatat
     1381 tttttacatt ttgctaaatc gttgtttgat tacagtctaa aagctctata atctctttat
     1441 aagatttgcc catcagacga aatataatag attgaaacta gaatagtaca cctctacttc
     1501 taaaacattg ttagaaatcg atttgtcctg ttcttgtttc attttactat agaacgattt
     1561 gaaggcgttt ataatattta gctgtacgag agtcttttaa aagtgttttg atggtttgga
     1621 tttcttcttt agttgatttc atattactat tgtataatgc tttttgattt tagtttagta
     1681 ttatcgtaga aaatggactc ggtgctatgg atacaccgga tgagaatggt tatgtagcag
     1741 atgactatcg gattacttac ttagaggccc acatcaaggc catgcgagat gccatttacc
     1801 aagatggggt tgacttgctt ggttatacga cttggagctg tatcgatccg gtttcagctg
     1861 gcacagggga aatgaataag cgctatggct ttatctatgt agatcgagat aatgtaggaa
     1921 atgggactct caaacgtagc aagaagaagt ccttctattg gtacatgtca tttatagcaa
     1981 tggtgtaagc atcgagtaga gcacatttgt ttactatttt tataaaatct tctccctgct
     2041 ttataagatg taaaaaagag ttcaaatgga actctttttt tgctataatg aggggagaaa
     2101 aatcagacag gagattaaga tgtcagaacc attattttta caatcagtta tgcaagaaaa
     2161 aatctggggt ggtaccaagt tacgtgatga gtttggctac gacatcccaa gtgaaaaagt
     2221 cggagagtac tgggctatct cagctcatct aaatggtgtt tctaaagttg ccaatggtcg
     2281 ttacgaggga acagatcttg ctactttgta tgcggaacac cgtgaattat ttggcaatcg
     2341 tccagaacct gtatttccgc ttttgaccaa gatcctcgat gccaacgact ggctcagtgt
     2401 ccaagttcac ccagacgatg cttatggact cgagcatgaa ggcgaacttg gaaaaacaga
     2461 atgttggtac atcattgcag cagatgaagg ttcagagatt atctacggtc acaatgccaa
     2521 gtcaaaagaa gaactccgcc agcaaatcga ggataagaac tgggatgcgc tgttgacaaa
     2581 agtgccagtt aaagcaggag atttcttcta tgtaccaagc ggtaccatgc acgctatcgg
     2641 ggcgggtatc ttgattcttg aaacccagca gtctagcgat accacttacc gtgtctatga
     2701 ctttgaccgt aaggatgaca agggcaattt acgtgaactt caccttgaaa aatctatcga
     2761 tgtcttgaac attggtgaac ccgcaaatag ccgtccagtg acaatcaaag cagatgatct
     2821 acgttccact ctccttgtat ccaatgattt cttcgcagtt tacaaatggg aaattactgg
     2881 aaaagttaac tttgaaaaga cagctgacta cagcttgttg agtgtcttgg ctggtcaagg
     2941 tcagctaact gttgacggga aaaattatcc aattcaaaaa ggcagccact ttatcctacc
     3001 aagtgatgtt gaagcttgga ctctggaagg gcaaggtttg gaattgattg ttagccatcc
     3061 ataaaaagaa agggcctgag ttcactactc aagtcctttt tgattacata aattgggcaa
     3121 tgaagaccac aatgatgatg attggaatga cgaaacgaag aaacgaagaa ggaagagcca
     3181 gacttggaac agtccttgtt tccatgctct ttcatcgaga tggagttcct ccattgcaag
     3241 agccttttta aagatatagc ctgtaaaaag tgaaaggtag agagctccaa atggcatgag
     3301 gagattggaa accaagaagt ccatagcgtc aaagaaggtc ttaccaaaaa tgtgaacatc
     3361 cgccatgaca ccgtaagata gggctgaagg aatgccaaag acaaaggtca aaattcctaa
     3421 aataacactc catttggcac gtttgctgtt atcctggttg gtgatattgc ccacattgat
     3481 ctccagcatc acgacagaag aagtgacagt cgcaaagagg gagcggacgt agaaaatggt
     3541 tccaaaaggc atcttgtcaa agagttgagg caagacgata aagagcaggc tgggtccccc
     3601 ttcagactgg atattgaagg gggatcgagc ttggaaaatg gctagacctg ccatgatgga
     3661 tatcgagata ttcatggcta cgatggagat tcctgactgg actagattgg ttttcttgtc
     3721 caagtaagaa gcataggtca acatgactgt aacccctagt gagagggcaa agaaagattg
     3781 tcccagagca tagaggagac cagcgctagt cagttttgaa aagtctggtt tgaggaagta
     3841 aagaacccct tccatggcat ttggcaaact gagagagcga ctgatgataa aaacaaagac
     3901 gataaagagc aggggcatca tgactttcga agctctttca atcccttttt gaaccccacg
     3961 tgatacaatg aagatattca ataggataaa ggccgcttga gctcctaggg caatggctgg
     4021 atttgaaatg attgaagtaa ataactgagc ataatcaccc gttccaccaa gttggaacaa
     4081 tttcccaaac tcaataccta gatagactag aatccatcct ccgataacac tgtaaaaaga
     4141 taagaggata aagagggcaa aggcgccaat ccaaccgata aagttgtact tgttattctt
     4201 gcccagtttt ccaaaggttt tgatagcgga aacgccagca ctacggccaa gggcaaactc
     4261 agccagcagg agagggaaac cgattaaaat agtggaaatg agaaagatta gtaaaaagcc
     4321 tccaccgcca ttagcagcag tcatgtaggg aaacttccaa acggaaccaa gcccgatggc
     4381 cagccagcag atgctagaat aaaaccaagt ttcgaccccc attgtgattt ttctgacata
     4441 gttaaactct gatctccttt ttcaaaataa gaaaaagcta cttgagttgt caaatagcct
     4501 tctgtttgat tgatagactt gactatccta tcatgttttc taaggtctgt caagaaaatc
     4561 attttcaagt tttcacacct ttctcaaaaa agttaaaaaa ttttctcaaa aacgcttgac
     4621 tctgacctaa ggggaggggt tatactatca ttgtaaggag gaaatcatgt accatataaa
     4681 agaagctgcg cagctttcag gtgtctctgt caagaccctg catcactatg acaagatagg
     4741 actcttggtc cccttaaagt cggaaaacgg ctatcgaacc tacagtcaag aggatttgga
     4801 acgccttcag gtcattcttt actacaaata tctaggcttt tctttagaga aaatagcaga
     4861 gctgttaaag gaagaaagga cagatttatt gccccatttg actaggcagt tggactatct
     4921 aactcgcgaa aggcaacatc tggatacctt gatttccacc ttgcaaaaaa ctattcaaga
     4981 acaaaaagga gaaagaaaaa tgaccattga ggaaaaattc acgggattta gctatcaaga
     5041 caatcaaaaa taccaccaag aagcggtaga gaaatatggt caagaagtca tgggacaagc
     5101 gctcgaacgc caaaaaggtc acgaagacga ggctacggcc gcctttaacc aagtctttca
     5161 aactttggca caaaatcttc aagttggttt acctgcaaca gcaaccgaaa accaggagca
     5221 agcagccaag ctcttgcaag ccattcgcac ttatggattt gactgctcta ttgaggtatt
     5281 cggtcatatc ggtaaaggtt acgtctataa cccagagttt aaggaaaaca ttgacaagtt
     5341 tggttctgga acagcccagt acacgtcaga tgccattgca gcttacgttc agacaaatgc
     5401 agaaaaaata ggctaggaat ttcctagcct attttttact tcaaatcata aagccagtcg
     5461 tcaccgtttt tgtagtaaaa gaattcactg agatcttctt ctagaaacac acgaagcata
     5521 tcagacatat catcggttgc aagttttaga tgagaaagat tttcaaagtc ctcccaccaa
     5581 actttccctt cgtctgaaga ctggagttca ccagtaaagt gttctgtctt gtaaaaaagg
     5641 acgacataac gataatcctt gtcgtcatac cagtttttga taccacagag ttggggtttg
     5701 gaaatgatca gaccagtttc ttctttcact tcacgaatga cagcatcgac aaaggattcg
     5761 ccacgttcaa catgaccacc aggaaaagta atgccaggcc agtcgggact aactcggtct
     5821 tggaccagga ccttatctcc gtttttaatc atacacatgt taacaaattc gactgcctct
     5881 cttctgttca ttcttcacaa cctttaatct ttaatcataa tgcagacttc ccgccaccca
     5941 gccggtacag agggcagaag tgatgttaaa gccacccgtg tgggcattga tatccataac
     6001 ttcgcctgca aagtggaggc caggtaccag cttactttca agggttttag gattgatttc
     6061 cttgagactg actccaccct tggtaacaaa ggactttgca agggacattt ttccagttac
     6121 aggaatttta agttctttaa tggactgaac aagttgttct cgttcctttt cagtcagctg
     6181 tttgactttt tcaggatatc cttgtacaaa aaattcggcc aagcgttctg gtaacaaggt
     6241 ttttaaagcg tttttcaagg atttttcccg attttcttct agaaatgtaa ccaagtcctt
     6301 ctcagaaagt tgaggcaaaa catcgagtga gagaacctcc ccacctttga caaagctaga
     6361 catgcgtagg gcagcaggac ctgacaaacc aaagtgggta aagagtaaat catgagtgat
     6421 gacatgctta ccataactta gggtcacatc gtccagagaa atcccttgta aggctttatg
     6481 tggaaaatct gttaataaag gactttcagc agcctcaagc tcggtgatgg tatgcttaaa
     6541 atggcgagca atctcgtgac caaaaccagt cgaaccagtc gaaggataag acttaccacc
     6601 tgttgtgaca atgagtttct cacaagtgaa ggtttgatcc gctgacttaa ggacaaactg
     6661 gtcatctact tttttaacag aaacgatttc tatttgagta gcaacttgac cacctagttc
     6721 ggtgattttc ttttccaaag cttcgataat agtccgagac ttgtcactgg ctggaaagac
     6781 gcgtccgtgg tcttcgacct taagtttaac accattttct gtaaaaaagt tgatgatgtc
     6841 atgattatcg aactgggaga aaacactgta aagaaagcgt ccgtttccag gaattccagc
     6901 tagcaggttg tctaagctac cattgttggt cacattgcaa cgtcccccac cagtcccagc
     6961 taattttttt ccaagtttcc gatttttttc gatgaggagg gttttctgtc cataaaagct
     7021 actggaaatc gtagccatca taccagcagg tcccccaccg atgacaatag tatcaaaatg
     7081 tttcatagct ctattgtacc acaaaaaaac aagagatgat ggtcacctct tgtcaagaat
     7141 gcaattaatc aatttcatag cccatcagca aaccgccatc ttctgcatag aaactgcaga
     7201 gaccagaggt tggtagaatt ttaatatccg cttgtgggaa ggtttcacgg attcgctctg
     7261 agagctgttg acaacatttt tcgttattgc gttgggccat gacaatacgg ccaccagcat
     7321 atccagcttt tactaactca tcataggcag cttgaactga tttctttgat ccccttgctt
     7381 tttgtagcaa ttcgagagtc ccagtttcac tagcttttcc gaccatacga atgttgagaa
     7441 ggccaacgac cgtaccgata agcttgctca aacggccgtt cttcaccaag ttatcgactt
     7501 tggctaggac aaagagcaac ttagtttttt cttgataggc ggtgatagct tcaaccactt
     7561 cttcaaaaga caagccctgg tcaatcaagt cattcaattt ttctacgagt aggtcaactt
     7621 caccaccagc agataaacta tcaatcacat gaatcttagt gtcaggatgg tcttccagat
     7681 aaatattctt tgctagttga gcactattgt gactgccaga aagggtacct gtgatggtta
     7741 ctaggaaaat gtttttggca ccttcaaatg ctcgcaaata gtcatctggg cttggacaag
     7801 ccgattttga agcttctgca gttgcataca tggtttccat catttggtca atatcgagac
     7861 tggcgtcatc aacaaagacc tgatcagcta cttgaatggt taaggggaca cttacaaagg
     7921 ttgtgttaat agctggtgtt ggcagttgac gataatcaca accagagtca gcaataattt
     7981 tccaagtcat agaaattctc catctttgtc agttatacat tgacaaaggt tctgtctttt
     8041 tttacaatta tatcatgaaa acccttgaaa caaaagcatc atccgtctgt tgtgacaagt
     8101 agaaagaaaa tgttatgtct gaacgtagaa tctctgaaaa gtctcttgaa aatctcagaa
     8161 aatcaaacca agaatccaat ttattaacca gagaagccat tgaaacagcc ctcttgcaac
     8221 tcttggaaaa aaaggagctg accaagatta gtatttctga attggtcaaa cgtgcaggtg
     8281 tttcgcgtgc ggccttttat cgcaattatg actccaaaga ggaaatttta gagagcgtct
     8341 ttaaacgaac tgtccacaat attatggaac agctgcatca ttacgattta aagacagacc
     8401 tttatttggt ttgggttcac cttttccggg aggccagaaa ggaagctaga gtgattcaat
     8461 tggccttgga ttaccatctg gaaaaaatct ttgtccaagc catgcaggaa tttctagaaa
     8521 aataccatgg gaaatcaaaa ggtgtcagct cttatcttca ttccttctgg agctcagcca
     8581 tcgtctctgt ccttctaaaa tggatcaagg atggcatgaa ggtaccagct gaaaagattg
     8641 cagatttagg tttaccattt tttaaaaaat agagaaaaag gagaaaagag atgactgaaa
     8701 aaagactggc ctgggatgag tattttgcag cccaagccct actaattgcg aatcgttcca
     8761 cttgtaaacg tgctaaagtg ggcgcgattc tggtaaaaga taataaggtt atttccactg
     8821 gttacaatgg ttcggtgtca gggactgagc attgtattga tcacgaatgt ttggtcattg
     8881 aaggccactg tgttcgcacc cttcacgctg aggtcaatgc tatccttcaa ggtgctgagc
     8941 gtggtgttcc caaaggcttt acagcctatg taacccattt tccttgtctc aactgtacaa
     9001 aacaattgct tcaggtcggc tgcaagcgcg tggtttatat caaccagtac cgaatggacg
     9061 actacgccca atacctttat caagaaaaag ggacagaatt gacccattta ccacttgaga
     9121 cagtacagac agctcttaaa gaggctgatc taatgtaaaa attatcaaaa ataaatggct
     9181 tagaaagatt tttaaaccgt tttttggtat aataagaaga ataaattgaa agaaggaatt
     9241 ccaaaaatgg gaaaaattga agttattaat cacccactga ttcaacacaa attgtcaatc
     9301 ttgcgtcgta cagatacttc tacaaaagct tttcgtgagc tagtagatga gattgcaatg
     9361 ttgatggggt atgaagtact tcgtgatctt ccactagaag atgtggaaat cgaaacacca
     9421 attacaaaaa cagttcaaaa acaattggca ggtaagaaat tggccatcgt cccaatcttg
     9481 cgtgcaggta tcgggatggt tgatggtctc ttgagcttgg ttccagctgc taaagttggc
     9541 cacatcggta tgtaccgtga tgaagaaaca cttcaaccag ttgaatactt ggtgaaattg
     9601 cctgaggaca ttgaccaacg tcaaattttt atagtagacc caatgttggc aacaggtggc
     9661 tcagcaatct tggctgttga ttctcttaaa aaacgtggcg catcaaatat caaatttgtc
     9721 tgccttgtat ctgctccaga gggtgtaaaa gcccttcaag aagctcatcc agatgtagaa
     9781 atctttacag cagccttgga tgaacgtttg aacgaacacg gttatatcgt tccaggtctt
     9841 ggagatgctg gagaccgctt gttcggtaca aaataagatc gaaaagaaga atgacttgga
     9901 aaagtatttc cagtcacgaa aggaggttgg gtttttgttt ctgtctaatg aaagcagagc
     9961 aaaaatttga ccttttttga ccaagatatt ataatagtct tatcttcagt cagttgacca
    10021 acaaaattaa aactcaaaag gagaaatgaa tgattcctgt agttattgaa caaacaagcc
    10081 gtggagaacg ttcttacgat atttactcac gtcttctcaa agaccgcatc attatgctga
    10141 caggtccggt tgaagacaat atggctaact ctgttattgc ccaattgctt ttcttggatg
    10201 cccaagatag tacaaaagat atttaccttt atgtcaatac accaggtggt tctgtttcag
    10261 ctggtttggc aatcgtagat accatgaact ttatcaaggc agatgtccaa accatcgtta
    10321 tgggaatggc tgcatctatg ggaacagtca tcgcatcaag tggagcaaaa ggcaaacgtt
    10381 tcatgcttcc aaatgctgaa tacatgattc accaaccaat gggcggtaca ggtggtggta
    10441 cccaacaaac tgatatggct atcgctgcag aacacttgct caaaactcgt aataccttgg
    10501 aaaaaatctt ggctgaaaat tcaggtcagt caatggaaaa agtccatgca gatgcagaac
    10561 gtgataactg gatgagcgcc caggaaacac ttgaatatgg ctttattgat gaaattatgg
    10621 ccaacaattc attgaactaa tgatgataga aggcaaactc gactgggttt gctttttttg
    10681 gtataatagg gagagatttc ttagaaagag gatttatcat gtttgaaaaa gtcaatcgct
    10741 ctggcttgat tatctatctt tactataatc gtgatgccaa aaaattgcag gattatggag
    10801 atattaccta tcattccaag aaacatcgtt acttacaact ctatgttcca actcaagaag
    10861 tggagcaatt ggtcggacgc ttgagcaagg aaaaatttat taaaaaagtt agggtttgtc
    10921 atatccaaga gttggaaaca ccctttgtgg gcaatcttta tcgataggaa aacgttatca
    10981 tagaaaaagt tcaaggaaaa cattgacaat tttctgataa ttcggtatat tcttaacaga
    11041 ctatttaaga aataaggaga caaaaagatg aagaaaaaat ttgccctatc gtttgtggcg
    11101 cttgcaagtg tagcacttct tgcagcctgt ggagaagtga agtctggagc agtcaacact
    11161 gctggtaact cagtagagga aaagacaatt aaaatcgggt ttaactttga agaatcaggt
    11221 tctttagctg catacggaac agctgaacaa aaaggtgccc aattggctgt tgatgaaatc
    11281 aatgccgcag gtggtatcga tggaaaacaa atcgaagtaa tcgataaaga taataagtct
    11341 gaaacagctg aggctgcttc agttacaact aaccttgtaa cccaatctaa agtatcagca
    11401 gtcgtaggac ctgcgacatc tggtgcgact gcagctgcgg tagcgaacgc tacaaaagca
    11461 ggtgttccat tgatctcacc aagtgcgact caagatggat tgactaaagg tcaagattac
    11521 ctctttattg gaactttcca agatagcttc caaggaaaaa ttatctcaaa ctatgtttct
    11581 gaaaaattaa atgctaagaa agttgttctt tacactgaca atgccagtga ctatgctaaa
    11641 gggattgcca aatctttccg cgagtcatac aagggtgaaa tcgttgcaga tgaaactttc
    11701 gtagcaggtg acacagactt ccaagcagcc cttacaaaaa tgaaagggag agactttgat
    11761 gctatcgttg ttcctggtta ctataatgag gctggtaaga ttgtaaacca agcgcgtggc
    11821 atgggaattg acaaaccaat cgttggtggt gatggattca acggtgagga gtttgtacaa
    11881 caagcaactg ctgaaaaagc atcaaacatc tactttatct caggcttctc aactactgta
    11941 gaagtttcag ctaaagctaa agccttcctt gacgcttacc gtgctaagta caatgaagag
    12001 ccttcaacat ttgcagcctt ggcttatgat tcagttcacc ttgtagcaaa cgcagcaaaa
    12061 ggtgctaaaa attcaggtga aatcaagaat aaccttgcta aaacaaaaga ttttgaaggt
    12121 gtaactggtc aaacaagctt cgatgcagac cacaacacag tcaaaactgc ttacatgatg
    12181 accatgaaca atggtaaagt tgaagcagca gaagttgtaa aaccataata gaaaaatgtt
    12241 gaaataggga atgagccttt gactcactcc ctgtttcgat atttaatact cttcgaaaat
    12301 ctcttcaaac tgcgtcaacg tcgccttgga ttatatatgt gactgacttc gtcagtctta
    12361 tctacaacct caaagcagtg ctttgagcaa cctgcggcta gtttcctagt ttgctctttg
    12421 attttcattg agtataagaa cctatcaaaa agtgagggaa aaccctcgga attataaata
    12481 gaaagagtga atcttatgct ccaacaactc gtaaatggtt tgattctagg tagtgtttac
    12541 gcgctgttag ccctaggata taccatggtt tacggaatta tcaagctcat caacttcgcc
    12601 catggtgata tttatatgat gggagccttt atcggttatt tcttgatcaa ttctttccaa
    12661 atgaatttct ttgtagcgct tattgtagct atgctagcga cagctattct tggtgtcgtg
    12721 attgagtttc ttgcttaccg acctttgcgc cactctactc gtattgctgt tttgattacg
    12781 gctattgggg tttctttcct attggagtat ggaatggtct atctggttgg tgccaatacc
    12841 cgtgccttcc ctcaagcgat tcaaacagtt cgatatgatt tgggagcaat tagcttaaca
    12901 aatgtgcagt taatgatttt ggccatttcc ttgattttga tgattttgtt acaagtcatt
    12961 gtccaaaaga ctaagatggg gaaagccatg cgtgcagtat cagtagatag cgacgcggca
    13021 caattgatgg ggatcaatgt aaaccgtacg attagcttta ccttcgcttt gggttctgct
    13081 cttgcgggtg cggctggtgt tctgattgct ctttattata actctcttga gcctttgatg
    13141 ggggttactc caggtcttaa atctttcgtt gccgcagtac ttggtggtat cggaattatt
    13201 cccggtgcgg ctcttggtgg ctttgtgatt ggtctattgg aaacctttgc gacagccttt
    13261 gagatgtcag atttccgtga tgccattgtt tatggaatct tgttgttgat cttgattgtc
    13321 cgcccagctg gtatccttgg taagaatgtg aaagagaagg tgtaaacgat gaaggaaaat
    13381 ttaaaagtta atattctatg gttactcctt ttgttagctg gctatagctt gattagtgta
    13441 ctggtttcag tcggagtact taatctattc tatgtacaga ttttacaaca aattggaatt
    13501 aatattattt tggctgttgg tctcaactta atcgttggtt tttcaggaca attttcactt
    13561 ggttatgctg gtttcatggc gattggtgcc tatgcagcag ctattattgg ttctaaatca
    13621 ccaacctacg gtgccttctt tggagctatg cttgtagggg ctttgctttc aggagcagtt
    13681 gccttacttg tcggcattcc aaccttgcgc ttgaaggggg actatcttgc ggtagcaact
    13741 ctgggtgttt ctgaaattat ccgtatcttt atcatcaatg gtggaagtct tacaaatggt
    13801 gcggcaggta tcttagggat tcctaacttt acaacttggc aaatggttta cttctttgtc
    13861 gtgattacaa ccattgcaac cttgaacttc ttgcgtagcc caattggccg ttcaaccctc
    13921 tctgttcgtg aagatgaaat cgctgctgag tcagttgggg ttaatacgac taaaattaaa
    13981 atcatcgctt ttgtctttgg tgccattact gcaagtattg ctgggtcact tcaggcagga
    14041 tttatcgggt ctgttgtacc gaaagattac accttcatca actcaatcaa cgttttgatt
    14101 attgttgtat ttggtggact cggttccatt acaggtgcga ttgtttcggc tattgttctg
    14161 ggaattttga atatgcttct ccaagatgtt gctagtgtgc gtatgattat ttacgctttg
    14221 gccttggtat tggtaatgat tttcagacca ggtggactcc ttggaacgtg ggaactgagc
    14281 ctatcacgtt tctttaaaaa atctaagaag gaggaacaaa actaatggca ttacttgaag
    14341 taaaacagtt aaccaaacat tttggtggtc taacagctgt tggagatgtg actcttgaat
    14401 tgaacgaagg ggaactggtt ggattaatcg gtccaaacgg agctgggaaa accacccttt
    14461 tcaacctttt gaccggtgtt tatgaaccaa gcgagggaac agtaacccta gatggtcacc
    14521 ttttgaatgg gaaatcacct tataagattg cctctttggg acttggacgt actttccaaa
    14581 atatccgtct ctttaaagat ttaacagttt tagataatgt tttgattgct tttggaaacc
    14641 atcacaaaca gcatgttttt actagtttct tacgcttacc agctttttac aagagtgaaa
    14701 aagaattaaa ggataaagct ttggaattgt tgaaaatctt tgatttagat ggtgatgcag
    14761 agactcttgc taaaaatctt tcctacggac aacaacgtcg tttggaaatt gttcgtgccc
    14821 ttgctacgga acctaaaatt ctcttcttag atgaaccagc agcaggtatg aacccacagg
    14881 aaacagccga attgactgag ttaattcgtc gtatcaaaga tgaatttaag attacaatca
    14941 tgttgattga acacgatatg aatctggtca tggaagtaac agaacgtatc tacgtacttg
    15001 aatatggccg tttaattgct caaggaactc cagacgaaat taagaccaat aaacgcgtta
    15061 tcgaagctta tctaggaggt gaagcctaat gtctatgtta aaagttgaaa atctttctgt
    15121 gcattacggt atgatccaag cagtccgtga tgtaagcttt gaagttaatg aaggagaagt
    15181 tgtttccctt atcggtgcca acggtgcagg taagacaact attcttcgca ccttgtcagg
    15241 tttggttcga ccaagttcag gaaagattga atttttaggt caagaaatcc aaaaaatgcc
    15301 agctcagaaa atcgtggcag gtggtctttc acaagttcca gaaggacgcc acgtctttcc
    15361 tggcttgact gttatggaaa atcttgaaat gggagctttc ttaaagaaaa atcgtgaaga
    15421 aaatcaagct aacttgaaga aggttttctc acgctttcct cgtcttgaag aacggaagaa
    15481 ccaagatgca gctactcttt caggagggga acaacaaatg cttgccatgg gacgcgctct
    15541 tatgtcaaca ccaaaacttc ttcttttaga tgaaccatca atgggacttg ccccaatctt
    15601 catccaagag atttttgata tcattcaaga tattcagaag caaggaacaa ccgtcctctt
    15661 gattgaacaa aatgccaata aagcacttgc aatctctgac cgaggatatg tattggaaac
    15721 aggaaaaatc gtcctatcag gaacaggaaa agaactcgct tcatcagaag aagtcagaaa
    15781 agcatatcta ggtggctaaa acaatccagt ggattgtttt agtcggcaga tgaagattac
    15841 gaagtaatca tcaatatagt ccgggggccc tttttagtcg gtggatgaag attgcgaagc
    15901 aatcatcaaa tccagaggat tgttttagcc ggtagattga gattgcaaac aaatctgcat
    15961 ctacattgaa agctgaattt ctaataattg aaaaaatcga atgaaacgta tcttaccttc
    16021 attcagggag ctcgatttta gagctctttt tgctagctta ttcatacttt tttgaatttc
    16081 gaaaaagaaa tgtaagcgtt tgatagattt acaaaaagat tgtataatgg ggataagaat
    16141 agaaaaggag aagtctcatg gcagttaaag attttatgac ccgcaaggta gtttatatta
    16201 gtccagatac aacagtatct catgcagcag atttgatgag agagcaagaa ttgcaccgct
    16261 tgcctgttat cgaaaatgat caattggttg gtttggtgac tgagggaacc attgcacaag
    16321 caagtccatc taaagcaaca agtctttcta tctatgagat gaattatctt ctgaataaga
    16381 caaaagtaaa agatgtcatg attcgcgatg ttgtcactgt ctcaggctat gctagtctag
    16441 aagatgcaac ttatctgatg ttgaaaaata agattggtat tctccctgtc gtagataacc
    16501 atcaagtata cggagttatt actgaccgtg acgttttcca agcctttctt gaaattgcag
    16561 gttatggcga agaagggatt cgtgtacgct ttgttacaga agatgaagtt ggtgttcttg
    16621 gaaaaattgt ttctttgatt gtagaagaaa atttgaatat ctcccataca gtcaatattc
    16681 cgcgtaagga tggtaaggtc attatcgaag tgcaaatcga tggatcaatt gatttaccag
    16741 ccttgaaaga aaaatttgaa gcaaatggta ttcaagtgga agaaatcgct cgcacttcag
    16801 caaaagtctt gtaagaaggg aagcccaaag gcttcttttt tcatgaaaag gggattagag
    16861 aaaaagatgg aaagaaatga
//
LOCUS       DAVLYR010000024        16770 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLYR010000024 DAVLYR010000000
VERSION     DAVLYR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.024"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..920)
                     /locus_tag="VYO63_000691"
                     /pseudo
     CDS             complement(1..920)
                     /locus_tag="VYO63_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1341..1739)
                     /locus_tag="VYO63_000692"
     CDS             complement(1341..1739)
                     /locus_tag="VYO63_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671654.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAL
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(2021..2503)
                     /gene="greA"
                     /locus_tag="VYO63_000693"
     CDS             complement(2021..2503)
                     /gene="greA"
                     /locus_tag="VYO63_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1671655.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(2574..4229)
                     /gene="mltG"
                     /locus_tag="VYO63_000694"
     CDS             complement(2574..4229)
                     /gene="mltG"
                     /locus_tag="VYO63_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1671656.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGSETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(4310..4804)
                     /locus_tag="VYO63_000695"
     CDS             complement(4310..4804)
                     /locus_tag="VYO63_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671657.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(4801..5265)
                     /locus_tag="VYO63_000696"
     CDS             complement(4801..5265)
                     /locus_tag="VYO63_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671658.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(5275..6609)
                     /locus_tag="VYO63_000697"
     CDS             complement(5275..6609)
                     /locus_tag="VYO63_000697"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1671659.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEVEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(6620..7237)
                     /locus_tag="VYO63_000698"
     CDS             complement(6620..7237)
                     /locus_tag="VYO63_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1671660.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVQPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(7285..10383)
                     /locus_tag="VYO63_000699"
     CDS             complement(7285..10383)
                     /locus_tag="VYO63_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1671661.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVVVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLLQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(10476..11642)
                     /locus_tag="VYO63_000700"
     CDS             complement(10476..11642)
                     /locus_tag="VYO63_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1671662.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVVTFAKR"
     gene            complement(11650..12744)
                     /locus_tag="VYO63_000701"
     CDS             complement(11650..12744)
                     /locus_tag="VYO63_000701"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1671663.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDTRDLIADLRQALEG"
     gene            complement(12902..14584)
                     /locus_tag="VYO63_000702"
                     /pseudo
     CDS             complement(12902..14584)
                     /locus_tag="VYO63_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14756..16714)
                     /locus_tag="VYO63_000703"
     CDS             complement(14756..16714)
                     /locus_tag="VYO63_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671664.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
BASE COUNT         4771 a         3711 c         3054 g         5234 t
ORIGIN      
        1 tagtccaagc attttttagg atagttgtta atccactttt cgatgaatgc gacttctttg
       61 ggagtcattt tcttggttcc cttaggtaac tatctacgaa tgagcctgtt gtgattctca
      121 ttagttcccc tttcccaaga ggcatagggg tgcgcataat agatgtgctc ctcagaaaat
      181 atatcaaaca agcgattgaa ttccgttcca ttatctgccg tgatggaaag aatcttgtgt
      241 tgttttaaga tgagttttag agcctgattg accacctcag cacttttatt tggaatcaat
      301 cggatgatct gatgtctact ctttcgatcc gtcaagacaa gcaagcagta gtttttcgct
      361 ctcgtaagta gaaccgtatc aatctcataa tgcccattct ccaagcgaag attgatagct
      421 tcaggccgct gtgcgatgga ttgaccagca ggtttaaagt tgatgctagc ctgtttctta
      481 agcgcttttc cttttctagg gtaaagcaga tcctgtttgc ttaaccccaa ttttccatga
      541 tgaatccaat agtaaatggt tgaaattccc acgttaaccc ctttagccat caccatcatt
      601 tcaggcgaaa atttttggtt atgatagtgg agaatctttt cctttagttc cttggtcacg
      661 cttaatttcc tgaccgagcg cttgcgattg ttttcataag actgttgagc atagtcggca
      721 gaataaacct ctttgaagcg cccttttcca agacattgtc ggactgtccc acgcttgatt
      781 tcagtgtgga tagtttgagg aacttttcca agcagagagg caatttctct atttgatttt
      841 ccttcttttt tccatcgttc gattaagcga cggctatcga ttgtcaaatg tttacctttt
      901 gtagtataat ggttttgcat ctctgtgcct ttcttgtgtt tgtggttgaa caacaagtat
      961 aacacagagg tgttttctta tgcctacaag agctttcatt atttccattt tcttttggat
     1021 ttcactctat tctgaaaaac ttgtgtatat ttactgaagc tagcaaggct tacctgtaaa
     1081 tttaatgaaa gcaacacaaa atccgagagg ggaatctcgg attaatgtca atttaaaaag
     1141 atagagagtt tttagtttaa ataaattgtt ttaaaatatc aacaacatca cttcttttct
     1201 taacctgata agccttgatt cctaattttg gggctacgat tatattgtcc tcaatatcgt
     1261 ctagaaagac acaatttcta ggttataact ggtatttatc gatagttact catatacatc
     1321 agtccacctc catactcgcc ttacagattc ttttggtaat aatatctttg cctaatgtag
     1381 agatagtctt gcaaagaaaa aacttccttg tagccatgtt tctgataaaa gtccagtgcc
     1441 tggaactggt aagtattgac aaaggcaaaa caacaatttc gattcttagc ttcactttct
     1501 gcctgttgca atagttttga accgattcct tgccctcgca gttcctcttt tacaaacaaa
     1561 tactcgattt ctagccaatt tccaaaagtc tctgctatca aacccgcaag gagattgccc
     1621 ttttcatctt cgacataaag attaagtggc tcactttcag cctcttctct ttttgaacgg
     1681 ttataaacac gaatcagatt ccctatttct tgcgatttat gtgattcctt attttccaat
     1741 ctaaggtata atgaaatgaa ataaaacatg cgcaaatcga ttaaggaatt taatctaatt
     1801 tctaacaatg tcttagaaat caaagtgtac tattttaact tcaatgcatt atacatctaa
     1861 tactcaatga aaatcaaaga gcaaactagg aaactgacga agtcagtaac catacatacg
     1921 gcaaggcgac gctgacgtgg tttgaagaga ttttcgaaga gtagcaaaat ggaaaaagga
     1981 gtgagtgaag cacatcgcct ccccactcct ttttctgttt ttaggctgtt ttttcaacct
     2041 tcaagatttt tacatcatag ctaccaacag gcgtttcaat ggttgctgta tcacctgttt
     2101 tcttgccaat caaggcctgc ccaattgggc tttcatttga aaccttacct gcaaaggcat
     2161 ccgcaccagc tgaacctacg ataatataaa cttcttcttc gtcctcacca atttcttgga
     2221 tggtgactgt tttaccaatc gctacttcgt cctgggcaac tgcgtcgcta ttgacgattt
     2281 cagcatagcg gatttttgtt tctaagctag agatttgtcc ttcgacaaag gcttgttcat
     2341 ccttagctgc ttcgtactca ctgttttctg aaaggtcacc gtatgaacgg gcaatcttaa
     2401 tgcgttctac cacttctggt cgacgaacca atttcaattc ttctaattct ttttcaagtt
     2461 tttccttttc ctcaagggtc ataggatatg ttttttctgc catttttctc aactttcttc
     2521 tgataatatt ttctaaagaa aattatgtga agtatcacat aattttagtt tgtttagttt
     2581 aatttgctgt tgacatgttc agcgacattg cggtcgtggt cttcttgatt gttagcatag
     2641 tagaccttgc cttctgtgac atctgctaca aagtagaggt tatcgctctt agtttgattg
     2701 atgcttgact caatcgcatc cagacttgga ctatcgactg gaccaggcat gagacctaca
     2761 tttttataaa cattataagg tgaatcaatg ttggtatcaa tcgcaacatc ctcagctaga
     2821 ctgatatttt gccccagttt tccttgggca tacaagattg caatattact ttgaagtggc
     2881 atatcacgat tcaaacgatt gtagaataca cctgcaatga gcttacgatc ttctgtcttg
     2941 gcaccttctt tttcgaccaa ggaagcaatg gtcaacaact cattgacagt caaattttta
     3001 gatttgatag tactatagta aggagatagg ttcttatcca tagcagctaa catctcatca
     3061 atcaagctct caatagttgt gctttccttg atagagtatg tagctgggaa aaggtatcct
     3121 tccaaacgat aacgcgcacc gctgtctttt acaggcaaac tttccagtaa agtaggatat
     3181 ttcgctactg cttgactgat aaacgtctca tcttgaactt tagccaagaa agcctccgct
     3241 gtcaaagact ctttgaagtc accttgcaat tgacccacag cttgagcaat ctgatccaag
     3301 gtataacctt ctggaattgt caaagtcgca agtacaggtt cttgcggttc atctgttcca
     3361 cctttttgca actctttgag taagtcttct gtactcatac tcttttgcaa attgtagtaa
     3421 cctgctttca agtcggtata atttttatac ttggcataaa aactaaaaat cagaccatgc
     3481 tttaccaaac cagctttttc aagcgtcgta ccgatttctt gaacgtttga accttctgga
     3541 attccaaccg tcacatattt cttagaatta gcatcgatag gtaacaaaga atctagcacg
     3601 tactggtaac caaagtaacc acctgctgag agcaaggcaa ggaatacaag gaatgaaatc
     3661 acaaaagctt tcacatgtga cttcttctct ttcttaggct taacgggttt tgctccttct
     3721 ctacggctac gacgtgatgt atcgctgatg atatccactg tttctgtagc aggcgtaggt
     3781 gtttctgaac ctgcttgttc tgcttttttc tctgccgtcg tataggaaac agctaccctt
     3841 gttggggttt cattgtattc tctttcaagt ttcttaggtc taacaggacc tggacctggt
     3901 cttgatccac tttcttccgc tggagaagaa ggtacatctt gacttggatg acttggaaca
     3961 ccaggagttt ctctttgaat ctcatctgct ggagaagctg gtacaccttg acttgggtga
     4021 gtaggcacgg taggagcttt tctcataatc tcctctaccg ttgacaagga atcagccatg
     4081 agttcttcag ctgaaggttc atttgcagga gtgcgaacta ctgcctcatc ttctttcaga
     4141 acttcatcat agccttttac tttttctaaa tctctcagaa tctgctcttt aaagcttaat
     4201 ttctcttctt ctcttgactt ttcactcaaa agtttttcct ccttgttgat aatccataat
     4261 attatatctt aaaagtccaa aaaaagcaat ctatgatact tttcctaact tattttttca
     4321 tttcccagac catatcatac catgtttccc ctgcaaaggt tgactgggac tggccttcgt
     4381 tgacaaatcc atgtttttca taataggcga tgagatagtc atgacaggtt agattaatgc
     4441 catctctctc atcttcaaga gcaacttctt tcaaggctgt cagcaatttc tgacccagtc
     4501 cgagtccctg tgcctccttg gcaatagaca gacaggtcac agagatataa ccaccaggct
     4561 catgactata gtcttttatt tcttctgtaa aagactggtc ttgcagatgg cggtgcaggc
     4621 caactggtcc ttcgatataa cccatgattc ttccttcttt ttcagcaacc agaaaagagg
     4681 tctgaatttc tctcaaatgt gcttcaaaga cagaaggagg aatggcttct tcgaccgaaa
     4741 aattttcaaa ttcaagttca acaatccgat ccaaatcttc taatcttgct tgtctgattt
     4801 tcattgttcc tccagataaa agggattaaa ccaaatcata ctatagccct ggctagttgc
     4861 atagagcaaa gttgcttctt catcaacaaa accgttcatt tcaaaatagg aaagcagctc
     4921 atcaggactc tccaaacgaa tccctttgta atccagctca actgccacct ctttcaaggc
     4981 tgcaagaaga agtgttccca agccctgtct ctgatggtca gactcgatga ctaaagaatg
     5041 tacttttaga tattgcggat tgtctgactg gggacttgat aaaatatagc ctaaaagttg
     5101 attttcatcc ctagctagaa gaaaggtatc cgcacactta cggatacttt cttctaaaat
     5161 atgggaaagt tgctgctttt cagctggaaa agacgaggtc tgaagtgccc ctatctcagg
     5221 caaatcagac ttgcttgcct gaatgatctt aattggaatt tccatgggaa catcctattg
     5281 aacattgctt gtcaagttag acaagagacg ctcaaatgag tattcatagg tttggatgtc
     5341 tcctgctccc ataaagacgt aaacagcatt gtcatggtct aggagcggag aaacattttc
     5401 aacagtaatc acttggtgtt ttttgttgat tttgttggct agatcttcta ccttaacgtc
     5461 accatgatct acttcacgag ccgagccata aatttgcgct agataaacag catctgcttg
     5521 gtttaaagca tgggcaaagt cgtccaacaa ggcaatggtt cttgtaaagg tatgcggttg
     5581 aaagactgct acaatttcct tgcttgggta tttctgacga gccgcatcca aggtcgcaat
     5641 aatttctgtt ggatggtggg caaagtcatc gataatcact gtatcattga caattttctc
     5701 agtgaaacga cgtttaacac cagcaaatgt tttcaagtgc tcacgcacca agttcaaatc
     5761 aaatcctgct gtgtaaagaa gaccaataac ggctgtcgca ttcatgatat tgtgacgacc
     5821 aaaggttgga atgtggaatt gccccaagtt ttgtccacgg aaatgaacgg tgaaggttga
     5881 accagttatt gaacgaagaa gatcactagc tacaaagtca ttgccttcaa cttcaaaacc
     5941 ataataataa attggtgcat cagacgtaat cttacgcaat tcagcatctt caccatagac
     6001 aaaaagaccc ttggtgattt gtttggcata gtcgttaaag gcattaaaaa catcctcgag
     6061 acttgtgaaa taatctggat ggtcaaagtc aatgttggtg ataatagagt attctgggtg
     6121 gtaaggcatg aagtgacgct catattcgtc agattcaaag acaaaatatt tggcattggc
     6181 cgaaccacga cctgtcccat ctccaatcaa gaagctggta tctgtaatgt gagacaagac
     6241 atgagacaac atacctgtcg ttgaagtttt tccatgtgct cctgctactc ccatgctaac
     6301 aaagtcacgc ataaagctac ctagaaactc atggtaacgt ttgtagctga taccattttg
     6361 gtccgcatag gcaatttcga cgttgttatc tggacgaaag gcatttccag cgataatttc
     6421 catatcaccg tctagattct tttcatcaaa aggaagaatg gtaattcctg cctgctcaag
     6481 accgcgttgg gtaaagtagt acttttcaac atctgatccc tgaaccttgt gccccatctg
     6541 gtgcaacatc aaggccaagg cactcatccc tgatccctta attccgataa aatgatatgt
     6601 ctttgacatg ttttctcccc tattctgtca ttctggtcag attcaactct tgggcaaccc
     6661 gacgttcttg ttctgtttgt ttactttttt tattgtagat ttggctcttc tttagaaaat
     6721 cataattgtt tttctttgga gcaggtgctg acacttcttc attcttggta gggatagaat
     6781 gaacttcttc cgccaagata taatgagact gggtcaattt ttggctatat ttgacaaatt
     6841 caccaggatt ttccttttgg aaaggagttg tcggttgatt gccctgtcta actagactgg
     6901 gctgagaatg acgtctcgca aggctgaaat cctgagttag atagttagca gagcgtttct
     6961 ttttcaagtc tgcacgcgct tcttcacgcg ccacctccgc atagctcttt ccttcttttt
     7021 taacccctaa aggagctttt ttaggtttcc ctacttgctt ttcaatcggt tgtactggtt
     7081 tttcttcagc aataggagcc cattctaaat aatttttatc tcgatactca cccttgatat
     7141 tactgatcag atcagactca tcatagagat tcatgactgg catttcagtc aacatgacct
     7201 cgtcatctga caccaatgga aatcgttctt gtttcatttt ctatttcctt tcaacacttc
     7261 attatagcgt attgtcttga tttttcaagt gctggcttca gaaattccca aaatttctct
     7321 aatttctgct agggtcagac taccacgtga ctctgtgccg tccaatactt gtgacaccag
     7381 atgtttcttt tgttcttgga gttcctgaat tttttcttca atggttccct tggtcaccaa
     7441 gcgatagacc tcaaccgttt cttcctgacc catccgatgg gcacggccaa tggcttgcgc
     7501 ttccaccgca ggattccacc aaaggtcaac caagatcact gtatctgcac ctgtcaggtt
     7561 cagaccgacc ccaccagcct tgagggaaat cagaaaggca tctctttctc cttgattaaa
     7621 ggccttggtc atgtcttgtc tttccttggc tggggttgaa cccgtaattt taaaggaagt
     7681 caggcccaag tctggcagtt cttgttcaat tttttccaac attcccttga actgagagaa
     7741 aatcaagaca cggtgtccgc cgtctgccac ctgtagcagt aggtctcgga gactatctag
     7801 tttgccgctg gctccctgat aatcttccat aaacagggca ggagtgtcac atatttgacg
     7861 caagcgcatc aaaccagata aaatttccac acgacttcgc tgaaattcct gttctgacac
     7921 ttgagccaga tggtctcgca tctgttgtaa ctgggcaagg taaatagcct tttgctggtc
     7981 ttccagttca tttttataaa ccacctcaat caagtctggc aattcagtca gaacttcttc
     8041 tttcttgcgt cgcatcacga aaggcttgat aaactgagcc actcgctctg ctggcaattt
     8101 cataaattct ttcttgcttg gcaaaagtcc gggcatgacg atttggaaaa tagaccacaa
     8161 ctcacccaga tggttttcaa tcggagttcc tgacaaggca aagaccgacg gcaccacaaa
     8221 ttgtctcaag gtctgggcaa tcttggtctg ggcatttttc atgacctgag cctcatctaa
     8281 gaaaaggaag tcaaaggcca tcccctgata aaactcactg tcctgacgga aggtggcata
     8341 gctagtcaca tagatttgat ggctctcggc aagaatctcc tcacgacttg ctttcaaacc
     8401 atgaacaaca accacatcca actgtggggc aaatttctga aactcatctg cccagttgta
     8461 aatcaaacct gacggagcga gaatcaagac tcgactttct tttgtcactt gactagtcaa
     8521 aaaagcaatg gtctgaaggg ttttcccaag tcccatatca tcagccaaaa tcccaccaaa
     8581 accataatga tggagcatct gcaaccagcc aattcccttt tcctgataat ctcgcaagtc
     8641 agccttgacc tgagttgctt gcaaaggaaa gtcctctgga tgcgtcaaat cctgggccag
     8701 attctggaat tcttgtgaaa aagaaacacg gtctcgccct tcaaagagat gagctaaact
     8761 gtaggccaag gatttccgag cctgcaaggt tccatctttt aattcaaatt gccccagttc
     8821 ctggagattt tggcgaattt tcttggtttc ttcatcgaaa aagtaaactt gattagacga
     8881 atcaatataa aaatcctgat tggcaaccaa ggcctgcatg gcttggtcga tttcctcctg
     8941 ggcaatattt tgaaaatcaa actggatttc caagagacct cccttggagg caatctgcac
     9001 ctgaggactc gctaggctat aaagctcttc tagtttatct gataggtcaa catgcccgag
     9061 tttttcaaag actggaatga tatcatgaaa aaaatgatag acagactccg cttttaaggc
     9121 ctgacgccaa gattgaaaat cggcttcaaa gcccgcagcc aaacagactt ggaaaattct
     9181 ttcttctaag tccgcgtcac ttgaaaaggg taattcttct agctcttgtc ggctagatac
     9241 ctgtctattt ccataatcaa actgaatttc taaacgaatc cgattatctt cttccctgtc
     9301 aaagtaaaaa gagggcgcaa aagttttgat ttgtagacgt tctggagctg aaacggtgcc
     9361 catctggata aaaagagtca gacaggaggc caatttgtct cgatcactgc tatcaaattg
     9421 caggtatttc tttccttgtt gacccacagg taacgcttta atttccttga gaagacgcat
     9481 ctgctggtct gttaaaaaat aaacctgacc tttatggaaa agtactgctc cttgataaaa
     9541 gacattgacc ctaggactct cactgatttc catttcaaaa tagtccgagt attctgttac
     9601 tgtaaaggca aatagattgg catcagcatg catatcctga aaaagcaggg tttggtagct
     9661 atccacttga tggtcaaatt gaaaatgggg caaggccatc agtaaattca caccctgctc
     9721 aaaaaaggtc agagggaaaa agaggtgccg accttggttt tggaaaaaga ggtctggaac
     9781 cagcccttcc tccgttagtc cgtgcaagaa agtcaaaagt tcttggctgg catcatcaaa
     9841 ggcttcccaa gaaagagact cctcataaat cttgccaatc atatacgact ttctctgctc
     9901 gacaatcctt aaaaaaagtg gaatatcccg aatgacatag tatttttggc tattgatttg
     9961 gccgattctc agagtccaca agatatgatt ggttcctgct tccacctgac ccacagctga
    10021 taactcatag gcgcattctg attttggaga taaaattcga ttcaaaaact tgccacccaa
    10081 ggtcaccttg gtttcaacgg cctctttttc ttcatgacct tcttccagac tccacaagat
    10141 ttcctgacca cgctcatcat ttttcagaaa atgctctagc gctgccaaat gcacacagta
    10201 gcccctcttt tgaaaagaat cacaggcaca aaaaaccaaa tcatcctcta aactatagcg
    10261 cagttcttct tctgcaacgc gagcgtagag ccgattgttc ttttccttga tgatatcaac
    10321 cttaccagtt tcataaaggg caacaccttc gatacgaact ttccccggaa tcaatttagc
    10381 catattttca cctttacctt atctttttat tataccatat tttcgcctat gaaaatagcc
    10441 ttctaggaag acttttctcc tagaagactg gatttttaac gtttggcaaa agtaaccaca
    10501 atccgctgac agacttcctg caacagagat ttgggcatag ctatattgat gcgggcatgg
    10561 agacttcctt cctctccaaa atccaaacca cggttgagga taaccttggc ttcatttctc
    10621 aacaactctt gcaatgtttc atcagtcagg tcataggctg aaaagtcaag ccaaatcaag
    10681 taggtacctt gcggtttcat gaccttgatt ttagtctctt ttccaaatag atctaccaca
    10741 taattgatgt ggtcttcaaa gacttgcttg agttcctcta gccaatcttt accgtatcga
    10801 taggcagctt ctgtcgccaa ataacccaag cctgaaattt catgctgatt attggccaac
    10861 aggcgtttct ggaaagccag tctcaactta ggattttcaa tgactgcata ggaatttttt
    10921 gttccagcaa tattaaatgt tttagtggca ctgctcaaga cgatagcaaa atttttgaag
    10981 gcaggattga tggtattgaa agactggtgt ttgtgaccaa agagggtcaa atcttggtga
    11041 atctcatccg aaactaacaa aacaccgtgt ttttggcaga gttggccaat cttctccaac
    11101 acttcttttt cccaaacacg tccaccagga ttgtgaggat tgcaaagaat atagagttta
    11161 acctcctctt ccaccaaatc cttttcaagt tggtcaaagt caatctcaaa cagaccatcc
    11221 ttttccacta aggaattagt aatcaatcta cgattattca acttgacact gcgagcaaag
    11281 ggtgggtaga caggcgtgtt aattaaaacc gcctcgcctt cttttgtaaa ggtttgaata
    11341 gctgttgaga tggctggtac cacaccctcg ataaagacaa gagcctcttt gtcaaagttg
    11401 taaccgtatt gtgtagcttc ccacttttga acttccttaa ttaagtcttc actggcatag
    11461 gtataaccat aaaccagttg gtctgcgtaa gtttgcacgg cttggcggat ttcaggcaag
    11521 accacaaagt ccatatccgc tatccaagct ggtagaactt cactatccgt ttctgtttct
    11581 ttccatttat aggtatggtg ccctaaacgg ttgggcaggc ttgtaaaatc atattttccc
    11641 atctttgtct tatccttcta aggcttggcg caaatctgca atcaaatctc tagtatcctc
    11701 aatcccaata gacaaacgca agaggtcatc tgtcaaacca taagaatggc gtacttctgc
    11761 tggaatatca gcatgagttt gagtcgttgg ataagtaata agactttcca ctccgcccaa
    11821 actttccgca aaagagaaga ccttgagact gttcaaaata tgaggaatgc gtgtttcatc
    11881 ggctacttta aaggaaatca tgcctccacg accagtgtag agaacttcct taactgctgg
    11941 agaatccttc aaaaaggcaa ccacttcttg ggcgttagct gttgagcgct ccatacgaag
    12001 agacaaggtc ttgagaccac gaagcaactg gtagctgtca aatggagaca agactgcccc
    12061 tgttgtattg agattgtaaa aaagcttctc gtatagttct aaactattgg tcacaaccac
    12121 tccagccaag acatcattgt ggcctgctag atacttggtt gctgaatgga gaacgatatc
    12181 tgctccatct tcaatcggac gttggtagat agggctatag aaggtattgt ccaccaccac
    12241 tttggcaccc ttagcatgag ccaattttgc tagtttttcg atatcaaatt ccaacatcaa
    12301 gggattggtt ggggtttcga tatagagaac atccacatcc ttttctaact cagcaatcaa
    12361 ctcttcttct gtattggcat aggtaaaatg gaaacgacct tcctgctcca cttggttaaa
    12421 ccagcgaaaa gaaccaccgt aaagatcacg cactgccaag accttacttc ctactggaaa
    12481 gccgctaaag gccagtacaa tagctgacat ccctgagcta gtcgctaggg catagtctgc
    12541 tgactcaata gccgccaaga cttcctcagc cttactacga gttggatttt tagtgcgcgt
    12601 atagtcaaac ccagtagatc gaccaaactc tggatgctga taggtcgttg aaaaatgaag
    12661 cggtgtcacc aatgcacctg tcgcttcatc tgacttaata cccgcctgag ccaaaattgt
    12721 gttaatgtgt aattccttgc tcatacaata cctccaaatc tatagtaact attgtaccac
    12781 ttattttcat caactcattt tcttcttttc aagagctagt tatagtttca aactatataa
    12841 aaagggagtt tttcctgctc cctttaatag actataaaat ggtgaatctc aaaagacacc
    12901 ttcactctat catttgctcc tgcacaaaac gagcataacg ctcatgattt tccagtagtt
    12961 ccttatgagt tcctgagcca gtgattttcc ccccctctaa gaagaaaata caatccacat
    13021 cttttaccgt tgacaaacga tgcgctataa tcacaaccgt cttctccttt aatgcagaat
    13081 agaggatact gataatcgca tactcagaat ccgcatcaag attagcagtg gattcatcaa
    13141 atataagaat ttcagcatct tttaagtagg ttctagctat ttgaagtctt tggctttgcc
    13201 cccctgacaa gaatcgtccg cgttcaccaa cttcagtatc tagtccctct ttcatggagc
    13261 gaatctcatc acctagtgat actaagtcta gcactttcat caattcatca tcagttacta
    13321 agcgattcaa accgagacaa agattgtcac gaatactgcc agataagact gcattatttt
    13381 gtgaaaccca agcgatttta cttctccatt cttttaagtt aaaatcatat atacttgatt
    13441 gctccattag aatatatcct gaaagcggtt tataaaaccg ctctaacaaa cgcacaatcg
    13501 ttgattttcc tgatccagat ggtccaacaa aagcaatttt ttgccccttg aaaattgaac
    13561 aagtaatatc ctttaagaca ggtcgatttt catcataacc aaaatagaca tggttaaaat
    13621 tcaacccttg tcctgatacc gattttcctc actcaaattt ttctttagga actgcaagca
    13681 agttctccag tgcaactgaa gatcccttgc tcctagaata aatagttaca aaattagcta
    13741 tattactaat aggattaagt aattgaaaga ggtaaatcaa aaacgaaacc aaggttccca
    13801 cagatatata tcctgcgctg acccgataac ccccataggt tagcatcaca actatagtcg
    13861 caaagataaa taagagagca aacggggtct caaaagaagt aaccctatct gatttcagtg
    13921 aattgttttg taccctttca atacaattat ccaaaacatc ctgtacactt ttctctgctt
    13981 ggttagtctt aattaattca tgttcttgaa tcttttcagt caattgccct gttaaaaatc
    14041 cgatttcctc ctgtaaacga cgactatact tttcactgat attggaaagg ggcaagataa
    14101 taaacatcat acaaggaaga gtgatgaata aaagtagaga aagattccaa tcaagactaa
    14161 ataagactac aatggaacca agtaccataa ctaaactcag aataatattt gggaaagtcg
    14221 taattaaaaa ctcacgaatg acactcgtgt cattgacaat ggcagaagtc aactccccac
    14281 tttggctctt atcaaagaag gatttctctg cataaatcaa cccctctatc acttttttcc
    14341 tgatttttgc tatctttttt tcacccgatt gactaaacag atagtaacca atagaagaaa
    14401 acaaggcttg accaataaaa atcaaaaacg attgaaatac tttggaacct atattttcaa
    14461 tagaactccc atctattaaa ccctttaaga taaggggaag caacaaagca agtagactag
    14521 acagaacaag taagaaactc cccataatca ccttagtatc tactcttaat aattttaatt
    14581 tcataaatac tccttataat atttcaacgg ataaagtcga gaataactca atttgaggat
    14641 aaaatctaat aaatcttcct ataacaaaac gcataacatc taggatttta tatacctgat
    14701 attatgcgtt tttaagcaca aagacttctt acacaaactt atctacaatt agattttatt
    14761 tgacatgttt tgccaattct tcttgggctt ttttattgga ttcttctttt tctttcaacc
    14821 atttttctct ggcttttgca tattcgtctg ttgtgacaat cttatcttgt actttgaggt
    14881 atttatatga ttcaacccct tttgtaccgg ttaaaccata ggcagcagca aatggtacgg
    14941 ttcttctcaa tgatggtgtt cccccacgcg aaacacttgg aagaactaaa gaactatcaa
    15001 tcaaccaagc ttgaatatca gcatatttct cataacgttt ggctggatct tgctctttat
    15061 tagcttcttc caacatttga gtatagacat ccagtccaac tgccttagcc ttgtcattgg
    15121 cttctccagg ctctagtcca agattttgta gaactcctcc actattagta ttaaaaatat
    15181 cgagataggt tgacgggtct tgataatcag gtccccaacc gccatgatat aaatcatagt
    15241 ctttctgagc agctgtttga gcaaagtagc ctgaactgtc aaactcatct gatgttaatt
    15301 gctgaatgtc aatcactaca ttatcagaac ctaaaacaga ttcaattgat tgtttgatag
    15361 aactaactcc ttgtatgcct actttatctg ttacttccac agtcttatcc aagtggattg
    15421 ggaattgaac accctttgct tcgagttctt tcttagcttc cgcaaactta gccttggctt
    15481 tctcaggatt gtagtaaggg tcttgaccat ccgcaaagtt gataccttgc cattccttac
    15541 catagttgac catcttagag gctacaactt caccaaagtc ttttcccttg atactgacaa
    15601 agtttggagg aaccactagg ttacgcaaaa tctttgttgc accttctttc ccttcagact
    15661 gagccccata agatgttctg tcaaaagcaa aattgatagc ctgacggaag tttttattga
    15721 gaactgcttc ctgagtcgat ttcttttcaa tgtcacttgt tttagaagta taattgtaag
    15781 acttcctatc taggttaaaa ttaaagaaat atgaagttga attttgcata ctatagatga
    15841 tattgttttt gtatttttct ttaatccctt catagctgga gctgttagga aaaagacgag
    15901 ccgtagtata agcaccagct gtaaaattac gttccagtga ttcttggtcg ctaccatcat
    15961 agtaggtcaa tttcacatcg tctacaaaga cattcttagc atcccagtaa ttagggtttt
    16021 tcttatattc aatagcagat tttgagacaa gtgctttcat caagaaaggt ccattgtaca
    16081 aaatactaga tggatccgcc ttcccaaaat catccccttt tgatttcagg aaatctgcat
    16141 taacaggaaa aagtatcgtt gcaagtgttt ttgaattcca gtaaagttct ggtttaacca
    16201 aagtatattg aaccgtttgg tcatcaagtg ccttgacacc gacagttgaa aagtcgcttg
    16261 ttttaccagt gatatagtca tccaaaccag caacagagtc ctgcactaga tacaaggctt
    16321 ctgatttttt atcagctgca tattgcaaac ctgtcacaaa atcctgggca gttacaggcg
    16381 catattcttc tccctcagaa gtaaaccact tggcatcctt acgaagtttg taggtatagg
    16441 tcaaaccgtc ctgagaaaca gtccaatcct ctgctaatga tggaataata ttcccatatt
    16501 ggtcattttc taataacccg tctaccaaat ttgcaacaat atcggatgtt gctgcgcggt
    16561 tttctgctag atagttcaag ctagatggat cacttgaata aacatagttg taggtttttg
    16621 acgccgtgct agaatttcca cacgcgctca ataaaactcc tgtacccagg acaagacctg
    16681 ccaaggttag atatttgctc ttagactttt tcatttccgg aacctcctgc tttaaatatt
    16741 tgttttatta tacaactctt ggtttgttta
//
LOCUS       DAVLYR010000025        16361 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLYR010000025 DAVLYR010000000
VERSION     DAVLYR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.025"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(526..765)
                     /locus_tag="VYO63_000704"
     CDS             complement(526..765)
                     /locus_tag="VYO63_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1671665.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(785..1057)
                     /gene="rpsP"
                     /locus_tag="VYO63_000705"
     CDS             complement(785..1057)
                     /gene="rpsP"
                     /locus_tag="VYO63_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1671666.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            1227..1424
                     /locus_tag="VYO63_000706"
     CDS             1227..1424
                     /locus_tag="VYO63_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671667.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            1903..2124
                     /locus_tag="VYO63_000707"
     CDS             1903..2124
                     /locus_tag="VYO63_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671668.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            2207..3010
                     /locus_tag="VYO63_000708"
     CDS             2207..3010
                     /locus_tag="VYO63_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1671669.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(3066..4607)
                     /locus_tag="VYO63_000709"
     CDS             complement(3066..4607)
                     /locus_tag="VYO63_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1671670.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(4609..5091)
                     /locus_tag="VYO63_000710"
     CDS             complement(4609..5091)
                     /locus_tag="VYO63_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1671671.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(5091..6176)
                     /gene="rlmN"
                     /locus_tag="VYO63_000711"
     CDS             complement(5091..6176)
                     /gene="rlmN"
                     /locus_tag="VYO63_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1671672.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITISTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(6197..6727)
                     /locus_tag="VYO63_000712"
     CDS             complement(6197..6727)
                     /locus_tag="VYO63_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1671673.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(6884..7489)
                     /locus_tag="VYO63_000713"
     CDS             complement(6884..7489)
                     /locus_tag="VYO63_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1671674.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(7662..8699)
                     /locus_tag="VYO63_000714"
     CDS             complement(7662..8699)
                     /locus_tag="VYO63_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1671675.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(8733..9668)
                     /locus_tag="VYO63_000715"
     CDS             complement(8733..9668)
                     /locus_tag="VYO63_000715"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1671676.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(10221..11411)
                     /locus_tag="VYO63_000716"
     CDS             complement(10221..11411)
                     /locus_tag="VYO63_000716"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1671677.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(11788..13131)
                     /locus_tag="VYO63_000717"
     CDS             complement(11788..13131)
                     /locus_tag="VYO63_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1671678.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDRNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(13271..14086)
                     /locus_tag="VYO63_000718"
     CDS             complement(13271..14086)
                     /locus_tag="VYO63_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1671679.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(14138..16318)
                     /locus_tag="VYO63_000719"
     CDS             complement(14138..16318)
                     /locus_tag="VYO63_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1671680.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT         4865 a         3540 c         3020 g         4936 t
ORIGIN      
        1 attttatcac gttccaagga ttttctcaag ttggaggaag gggacaatat ctctactttc
       61 ccttcaataa tccttccaaa ttatgtttat gttggtaatt aacggctgcg gttttgtctt
      121 tctcaaagac agtcttggta aggtcaatat gattaatagc tacgattgcg acggtgtagt
      181 aaatgatatc agccagttct ctggcaagtt cctcgttcga atcctatccc ttcttttcga
      241 ccagagcgcc tattcaaaac ctcggctact tctccgactt cctccactaa cttcataaaa
      301 agaccttcat cagtccgaga ctgctgttaa tgttcgatta agtagtcttg gaattgccta
      361 aacgttcaat cttttatagt atattgaaac tagaatagta cacctttact tctaaaacat
      421 tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttattatttc attttactat
      481 atcttctatt ccacacaaaa aagcgagaca tccgtcccgc ccttcttatt tttcgtcaat
      541 aacgattctt acttttttgt attcagttgg gacagagtag acaatcgttc ttatcgcaga
      601 aatagtgcga cccttacgac cgattacacg acccacatcg ctttgatcaa gattcaaatg
      661 atattccaaa aattctggtg tatcctcaat cttgatagtt aaggcatctg gttgtgaaat
      721 taagggtttc acaatcgcaa taatgagatt ttcaatcgta tccatctgtc aacctacttt
      781 aaacttattt tgagaattta gaatcgtgga attttttcaa tacgccttct tttgaaagga
      841 tgttacgtac tgtgtctgaa ggttgagctc cattagccaa ccatgcaaga acgcggtctt
      901 ctttcaaagt tacttggttt tcagcaacaa gtgggttgta agttccaact gtttcgatga
      961 aacgtccgtc acgtggtgaa cgtgaatctg ctacgttgat acggtagaaa ggttttttct
     1021 tagaacccat acgagtcaaa cggattttaa ctgccatttt taaagtctca tttctttaat
     1081 tttttatttc ggtgaaatag ctgagctatt tagcacatgt tctattatag cagatttctg
     1141 gcaggtgtca agaaaaaaac ttgacagact ataaatttct taaagcattt agaaatttgt
     1201 tagaatagga aataaatgtt tgaaagatgc tatccgtgaa tactatttta gaaaaatttt
     1261 ataaagaaca ccaagtcaaa cccttcatat cgcctgaacg agagttggat acttggcttc
     1321 taagtcccaa gcgaaatatg gacttattag cagatgattc actagcagga gatatcattt
     1381 tactatggag aatccagttt ggtactttta ccactgaaac atgattttaa aaactcccat
     1441 tcgaatatga ttcaaaattc gcttagaatc aatgttttcc cactttttca aaaaataaat
     1501 tttcatctaa tttaaataac tttcaattaa gtgtgaattt tgcttaaaat cattaaaaaa
     1561 caccccatgg tgtgaaccat gaagtgttgt aaatgctgtg ttgtagctag ttcttagcgt
     1621 ttagagaact ggctagcttt acgagctttc ttaagatctg gtttccaaaa tactttgagt
     1681 acacttatgg tattgatgcc cctaaacatc taaaaactct ggtagaaaaa gggtatgctc
     1741 ttgtcgaaac ttcttttgac tcacttgacc atctaaataa taccatgaaa aagaatatcc
     1801 tgaagaagaa gggagttact ggactttcta aaatgaaatc ttgcgacctg gatcaagccc
     1861 ttcatgagca tttttcagaa gaagaattag ctggtcactt tcatgtcctt ctatggactt
     1921 ttttacaatg gcattgctat cacacccaat acctatctaa gcgcctggtt cgtaaacttt
     1981 attgcagctc ttcctctaaa tttcctaatt gttgaaccaa ttgcccgttt tatactaagt
     2041 tcttttcaga aaccatttac tggggaagaa gttgaagaag ttgaagattt tcaagatgat
     2101 gatgaaatcc caactattat ctaagccagt tctgtaaact actaatattt gaaatccact
     2161 tccttttagg gtacaatggt ataaatgaat ttttgagagg atcagaatga aaaaactagc
     2221 aacccttctt ttactgtcta ctgtagccct agctgggtgt agcagcgtcc aacgcagtct
     2281 gcgtggtgat gattatgttg attccagtct tgctgctgaa gaaagttcca aagtagctgc
     2341 ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa aacgccaatt tcccacaact
     2401 atctaaggaa gttgctgaag atgaagccga agtgattctc cacacaagcc aaggtgatat
     2461 tcgcattaaa ctcttcccta aactcgctcc tctagcggtt gaaaatttcc tcactcacgc
     2521 caaagaaggc tactataacg gtattacctt ccaccgtgtc atcgatggct ttatggtcca
     2581 aactggagat ccaaaagggg acggtacagg tggtcagtcc atatggcatg acaaggataa
     2641 gactaaagac aaaggaactg gtttcaagaa cgagattact ccttatttgt ataacatccg
     2701 tggtgctctt gctatggcta atactggtca accaaacacc aatggcagcc agttcttcat
     2761 caaccaaaac tctacagata cctcttctaa actccctaca agcaagtatc cacagaaaat
     2821 tatcgaagcc tacaaagaag gtggaaaccc tagtctagat ggcaaacacc cagtctttgg
     2881 tcaagtgatt gacggtatgg atgttgtgga taagattgct aaagccgaaa aagatgaaaa
     2941 agacaagcca actactgcta tcacaatcga cagcatcgaa gtggtgaaag actacgattt
     3001 taaatcttaa aaaccaaaaa aatacagtat ccacattcgg tactgtattt cttttactct
     3061 cattcttaag ttaaattatt aaaatcccat atttggtcta tccaaccttc ataaaagtct
     3121 ggctcgtggc agaccataag gatagatccc ctgtattctt tgagagcgcg tttgagctca
     3181 tcctttgcat ccacatccaa atggttggtc ggctcgtcca gcactaaaac gttgttttca
     3241 cgattcatca agagacagaa acgaaccttg gcttgctctc cccctgataa tacttgaatc
     3301 tggctttcaa tatgtttggt tgtcaaacca caacgggcaa gggctgcacg gacttctgct
     3361 tgattaaggg caggaaaggc attccagaca gcttcaagag gagtttggcg attaccgcct
     3421 tctacttcct gctcaaaata accaagttct aaataatctc cacgctccac ttccccagcg
     3481 attggcgaga taatgcctaa gagactcttc aagagagttg tttttccaat accattagca
     3541 ccaataatcg caaccttttg attgcgttcg aaggtaagat ttaaaggctt agtaagagga
     3601 cggtcgtaac caatttgcaa gttcttggct tggaagataa agcgccctgg tgtacgagct
     3661 ggtttgaaat caaaggatgg ttttggtttc tcactttgga gttcgataat atccatctta
     3721 tccaatttct tttgacgaga catagccata ttacgagttg caacacgggc tttattacga
     3781 gccacaaagt ccttgaggtc tgcaatctct ttctgctggc gttcgtaggc tgcctctagc
     3841 tgagatttct tcatagcata aacttcttgg aactggtagt agtcaccaga gtaacgcgtc
     3901 agctgttgat tttccacatg atagacaata ttaataacgt cattgaggaa tggaatatcg
     3961 tgcgaaataa gaacaaaggc attctcatag ttttggagat agcgcttgag ccaatcaata
     4021 tgctcagcat ccaagtagtt ggtcggctcg tccaacagca aaatatcagg cttttcaagg
     4081 agaagttttg ccaaaagcac cttggttctt tgcccacctg acaaagaagt tacatccgta
     4141 tccatcccaa agtccataac accaagagca cgcgctactt cgtcaatctt agcatccaag
     4201 gtatagaaat cacgactctc cagacggtct tgaagttctc ctacttcttc catgagagca
     4261 tcaacatccg cgccgtcttc agccattttc atatagaggt cattgatacg agcttcagct
     4321 ttgaaaagct catcaaaagc cgtacggaga acatcacgca ccgactgtct ttcagcaagg
     4381 acagagtgct gatccaagta accagccgtc acatatttgg accactcaac ctttccttca
     4441 tctggcagca ttttaccagt cacgatactc ataaaggttg attttccttc accattggca
     4501 ccgaccaggc cgatatgttc tcccttgagg agacggaagg acacatcttc aaaaattgca
     4561 cggtcaccaa aaccgtgact cagattttta acttctaaaa tactcatttt aattccttac
     4621 cttgttttta tgtaatcgtt tataaaggag ccaagccaga tagccaccca aagtgttggt
     4681 ccacaaatca tcaatctcaa agacgcgatt gaaatcaaag aaaaagtcca agattaattg
     4741 cgtacactcg attccaagac tcacaagaaa actaaaaaga aggacctttt ttgttttccg
     4801 caaatttgga aatagataaa ggagttggaa aatcagagga aaaaacaaga agacattgag
     4861 gatattttgt aaaaaaatcc aacataattg tccaatgtca ctcacttcgc ccagtttcca
     4921 gagagaattg aaaggagtca aaagaaaaac caggcgtcca agatgctgaa tacctggagt
     4981 tcccactccc acggtagatt gttcttgagg agtaaagcaa aaacagacaa tgcaaatgct
     5041 atagaaaatg actccccaga ccaaaatatg attataagtc ttcttcatca ttaaggattt
     5101 accgctgcga ctgccttctg gcggtcacgt ttcattgtgt tagagcgcaa ttgtccacaa
     5161 gctgcgtcaa tatctgtacc atgctcttga cgaaccacac agttgacccc ttttttctta
     5221 agcgtatcat agaaagccaa cacgcgctct ttgggactac ggctatattg gtcatgctca
     5281 ctaactgggt tataaggaat caagtttaca taagacaatt tcttgatgtt cttgagcaat
     5341 tcagccaatt ccaaggcttg ttctacaccg tcgttgactt cattaagcat gatatattca
     5401 aaggttacac gacggtttgt tgtctcaata tagtattcaa tagcagcaaa gagtttttca
     5461 atcggaaagg cacggttaat tttcatgata cttgaacgaa gttcattgtt gggtgcgtga
     5521 agagacacgg caagattgac ctgaacccct tcatcagcaa agtcacgaat tttatgagcc
     5581 aaacctgagg ttgaaatcgt gatgtgacga gcaccgatag ccattccttt atcatcattg
     5641 atagtacaaa cgaaattcaa gacattgttg taattatcaa agggctcacc gattcccatg
     5701 acaacgatat ggctgatgcg ttcatcctga ccacgctcat caaagtattt ctgaaccagc
     5761 atgatttgcg ctacgatttc accgttattg aggtcacgct gcttcttaat caaaccagag
     5821 gcacagaagg tacaaccgat attacagccg acctgagtgg tcacacagac agataaacca
     5881 tagtgttgac gcatgagtac agtctcaatt aacataccgt cgggcaattc aaagagatat
     5941 ttgactgtac catcagcaga ctcttgcaca atacgttgtt tcaagggatt gaccacaaac
     6001 tggtcattga gcttagcaat caaatccttg gaaaggttgg tcatttcttc aaatgactgc
     6061 acacgtttac ggtagagcca ttcccagatt tgatctgcac ggaatttctt ttctccctgc
     6121 tccaataccc attcctgcat ggtttgatgt accaaactat gaattgaggg tttcatttct
     6181 tctccttatt ctctactcac ttctgacgaa tgacaaaatg acgttgtccc ttgtcctctt
     6241 tctgagaatg ctggtctttc tgacgacgtc tatttttctt atctgcattc gactttcgtt
     6301 tagtttgagt cggtttcttt ccttttctag aaggtgtttc ttcttccgtc ttacgcattt
     6361 tcttgtcaaa tgatgctcgc ttaggggctt cattttctaa gacaaaatag gcacaaccat
     6421 aactacaata ctctaaaagg tagtcttgta aacgactgat tttttcaagt ttttcttctg
     6481 ttcggtcatc cttgtaaaaa cctcgtaggc gaagctgttc gttgctccag tcccccacga
     6541 tataatcaaa cttggttaag acttctgaaa aacgctgatt aaaagtcgtc acatcaaagg
     6601 catccttgat attttcaacc aaggaaaaag ctatcccttc cgtttcgacc ttgtccccgt
     6661 gtaaatggaa ctccggacca ggaaacttgt tataattgta taattcaggt gcaatttctt
     6721 ttcgcataga tatccttttt ccacgattac ttaatacttt attctaccat aatttctagc
     6781 agttagcacg tttctcataa aaatgaaaaa agtctgacga ttttgtcaga ccagaatctt
     6841 ataacctaaa aaagagaaga acaattcttc cctccaacta tcattattta gcagctgcgt
     6901 acaattcatc tactttattc cagttgatta ctgaaaagaa agctttgatg tagtcaggac
     6961 gcacgttgcg gtatttcacg tagtaagcat gttcccaaac gtccaagccc aagattggtt
     7021 ttttaccttc tgagattggt gtgtcttggt ttgctgttga agtcacttca agtttccctt
     7081 ctttgttgac aaccaaccat gcccaacctg aaccaaaacg agttgttgct gctgcagtga
     7141 aggctgcttg gaattcttca aatgaaccaa atgttgcatc gattgctgct gccagttctg
     7201 ctgaaggagc tgttttctcg ggagtcatca attcccagaa aagagcgtgg ttcaagtgtc
     7261 cgccaccatt gttgataagt gcttgacgga tatcagctgg gatagattct acatcagcaa
     7321 gcaaggcttc aaggtcttca ccgatttcag ggtgtttttc taaagctgca ttggcattgt
     7381 tgacataagt ttgatggtgt ttgtcatggt gcaaatgcat tgtttccgca tcgatgtatg
     7441 gttccaaagc gtcgtatgca tatggaagtt ctggtaagat aatagccatc tgtaatacct
     7501 ctttttcttt ctatatgaaa atgataacgc aaacattcac ttttttcaag ttttttgctt
     7561 atagataagg aaatcactga tatactttct aataaaataa gcaaatcagc aagaaaccca
     7621 cgacaagata atcctgacgc gggttgtagt ttcaacaaat gtcaattgac ctgactagca
     7681 atctgtaaga gtgccttttc aaaaaggaaa cctttttcat aaagacctgt cttaatctga
     7741 tagtctgtct caatcaaata ggaaatagct tgcttcagaa agctcaaaga aagtcctctc
     7801 gaatctctta atgcaaactt gatttgataa ggatttgggt tacgtcccag ataactacct
     7861 aaactacttg caatctgcga ttctgtttgg ccagactccg ccaaaatctt cacctgagta
     7921 aaagtccgaa attgtcccag catgactgca atcagtttga tttcatcttc cccttgcaag
     7981 gtcaagtctc tcactaaatc gcgcgcctga tccatctttt tagtcagaat aaactgagtt
     8041 aaatcaaaaa tattgtcctg caaggtcttg ggaattgcgt taacaatatc ctcttcctcg
     8101 ataacagaat tcgccttata ggactgtaaa aagagaagat ttttctggat ttcgctaaat
     8161 tgaaaccccg acttgatgag gagattttca aaagaatgat tggtaaactg cagaccttgt
     8221 ttctgactcc acttttggaa atactggcgc aattcttgtt cttttacttc tactgcatcg
     8281 aagaccttgg catcacgctt aagtaatttg actaaccgtc ttttgctatc cagctttcct
     8341 tctgcaaaga ttatcaactt ggttgttgga gaaggattgt caaggtattc ctcaaatgac
     8401 ttaagctcat catctgtcaa aaagcgtttc ttagcagtcg tgatatccat aaaataatct
     8461 aatatcacga ttttctcatc cgcaaagaaa ggaaggctga ccaactccag ttccacatcc
     8521 ttgtaaacta cttctttcat atcaaagtag gcaaagttga ggtcagcaga atcataccca
     8581 atctgtttca acatttgact cttcatcact tcaaactgac cctgatctgt ccctgtaaat
     8641 aggctcaggc tcggtaaatt tgataaagtc aacttctgac tttcttcaat ggctagcatc
     8701 gtcccttctt tcttctgatt tttcgattta atttagtcaa tatagcgcaa tttcccacgg
     8761 aaatcttcta agctctcgta gcctttttcc accatgattg ctttcagttc attggtaatg
     8821 cggtcaaaag cactgacgcc ttctttgtga agggtcgttc ccacctgcac catacttgct
     8881 ccacagagga tgtgttcaaa ggcatctcga ccagtcagaa cgccacctgt tccgataatt
     8941 tggatttgag gatttaaacg ttgataaaag gcgtgaacat tggctagagc agtcggtttg
     9001 atgtattctc caccaattcc accaaaacca ttcttaggcc gaataacgac agattcgtct
     9061 tctatataga ggccgtttcc gatagagtta acgcagttga caaacttgag cggatatttg
     9121 ttgaaaatag ctgccgcttg gtcaaagtga acaatatcaa aataaggtgg caatttaatt
     9181 ccaagaggtt tggtgaagta agcaaacact tctgccaaaa tccggtctgt tgtctcaaaa
     9241 tcataggcaa tctgaggttt acctggaaca tttggacagg aaagatttag ctcagtcaga
     9301 ccacgaaaat cactctcttg gacttttttc aaaatagtat gggtttcctc tggagacatg
     9361 ccgaccagag ataagaagaa agttcggttc gactcttttt cctgcaaatc taaaagataa
     9421 tccaaataat agtctaagcc attatttggc aagcccatag agttgatgga accaagtgga
     9481 acatcttggt agcgtggctc aggattcccc tgacggaagt ccaaggtcgc tgtcttagta
     9541 acaaaggttc ctgccgctga gtttttgacc tcttctaact cctctatcgt catacaagcc
     9601 acacctgctg cattcatcaa gcaattgtca aactcaaaac cagcaatttg tgttttcgtt
     9661 gataccatga ttctaatcct ccagatttct tttattgcta atattatacc ataattttaa
     9721 ttctaggttt actagattct gtggttctat ctatttattc ggaaaagaaa gtctagtaca
     9781 gaagtcatgc tatggaaaca gctagtgtat tggaagtttg aacaaggctt gggtaacaga
     9841 cttttgaatg aagtccaagc aacaaggaga aatctcaacc atgagtccat tccatgactc
     9901 tgttgctcta gaaaaccttg caatagtcaa gtgataacga ataaaacttt ggattgaaat
     9961 atccccatga ttatctatta cgaggagaaa tctcaacctt tatcattttt aaagaaatat
    10021 agtaaaatga aacaagaaca ggagaaatcg atcaggacag tcaaatcgat ttctaacaat
    10081 gttttagaag ttgaggtgta ctattctagt ttcaatctac tatacatgta aatctctact
    10141 ttcgcaaaat ggattttcag aacagtatcc cagtatagtt aaaaactata aaaaagagag
    10201 gacgttcccc tcttaaaatc ttattttact gcttctttca aagcatctac cttatccaaa
    10261 cgttcccatg gaagatcaat atctgtacgt cccatgtgac cgtaagccga tgtttgacgg
    10321 taaattggac gcttgaggtc cagcatttgg ataatccctg cagggcgaag gtcaaagatt
    10381 tgacgagccg ctttttcaag ttgactttca gctactgttc ctgtaccgaa agtatcgata
    10441 cgaacagaaa caggttgcgc aacaccgata gcataggcca actgcacttc tgccttctta
    10501 gcaaggcctg ctgcaacgat attcttggca atatagcgag ccgcataaga ggctgaacga
    10561 tccaccttag tcgcatcttt accagagaag gcaccaccac catgacgaga gtagccacca
    10621 taagtatcta caataatctt acgaccagtc aaacctgagt ccccttgagg accaccgatt
    10681 acaaaacgac ctgtcggatt gataaagaat tttgtcttat catcaagata agaagatgga
    10741 ataacttctt tgatgacctt gttaatcaca tcttgatgga tttgttcatt agtggcctct
    10801 ggatcatgct gagtagaaat aacgactgta tctacacgta ccggacggtc attttcatcg
    10861 tactcaactg taacttgtga ttttgcatct ggacggagat agctaatttc tccagactta
    10921 cgaagttctg ccagacgacg aaccaattta tgactgagtg caattggcaa tggcatgagc
    10981 tcttctgttt catctactgc aaatccaaac atgagccctt ggtcacctgc tccaatcaag
    11041 tccagtggat cttgatcagc atttccacga acctccaagg cttcgttaac accttgagcg
    11101 atgtcaggag attgttccac caaagatggg tgtactccca ccgtctcagc agaaaatcca
    11161 tattctgtat tggtataacc aatctctgca atggtatcac gaaccacacg gttaatatcc
    11221 acataggcat ttgtagaaat ttcaccaaaa acgtggacag aaccagtata tacagctgtt
    11281 tcagcagcaa cgtgcgcctc tggatccttt gctaaaatag catccaaaat cgcatctgaa
    11341 atttggtctg caatcttatc cggatgcccc tcagatacag attcagacgt gaataattta
    11401 cgttctgaca taaaaatgtc cccccttaaa aaatattgtt atagagttac aaagtactga
    11461 taggaatttc cattatctaa gagttactag cttcagaaat aaaaacgata cttttttatt
    11521 tctgaagcta gtaaggcgca tagggtgacc gcagtatggc ggcaaccgta gaaagacgag
    11581 caacctcgaa gccgtcattc tttgcgaatt gcaaactact gataggaaaa ctcttcgtaa
    11641 taaataccta ccatcttatc gggactatat aactttatca ttataccatt tttaatcgca
    11701 atttgcagtc tttaatgtag tgtttcggtt tagatagttc gttctcataa ataaaaaagt
    11761 tggagctcag tactccaaca atttctatta aaatgtttgg cgtttagctt tacgctctgc
    11821 acgaccgcga gcgcgatttt cagcacgctt agttttacgg cgcttttcat caaccgccca
    11881 ttgaattttc ttcttataac ccggtttgac ttttttcttt ttctttttaa ccaaaccaat
    11941 catttcgata tcaagtttat cttgtttttt ctcacggttg gcacgacgat cacggtcata
    12001 ggtatcttga aattccccat ctttgaccat cttaggacta aacttgattc ccaatttctc
    12061 caactcacgg atatccgagt catcacttgg ctgataaagg gtaatagctg tacctggtag
    12121 gccatttcgt ccagtacgac caacacggtg aacaaaaaaa gataagtctt gcggaatggc
    12181 atcattgatg acatggctga caccttcaat gtcaatccca cgcgctgcta aatctgttgc
    12241 gacaatatac tcaaaatcca gattttgcac ctgattcatg attcgcttgc gttcacgagg
    12301 ggcaatatct ccatggattt ttgcaacctt caagccttga gcagtcagat atgaatgcaa
    12361 ttcatcagca cgcgttttag tgttaacaaa aatcattgcc aaatacggct gcatcaactg
    12421 agtcaattgg taaatttgag cattcctatc atgtcccttg gtcgaaatca accaattatc
    12481 aatggtgtca gaaataaccg ttttagtctt aattttctcc ataacaggat ttgataagta
    12541 ttttttcaag aatggttgca gtttttgtgg gatagtcgct gagaagacca tgaattgcaa
    12601 gtcttttgga agactgccag caatcttatc aacaatttcc aagaatccca tatccaaggt
    12661 catatctgct tcatcaacaa caaatgtctt ggctttatga atagctaaat caccagattt
    12721 aaccaagtcg tagatacggc ctggtgttcc aataacaata tgaggctgat tgctttccaa
    12781 tttctcaatc tggcgagcct tatccgtacc acccacataa ttaaccacac gaacttcgac
    12841 atctgagtga gctgaaatct gacgcgctac ttggtaaatt tgagtagcca actcacgact
    12901 cggtgcagta atcactgctt gtacactatc gctagcttca tctaattgct ggaaaatcgg
    12961 taacaagaaa gtatgagtct tacctgaacc tgtttttgat tctcctacta ggtcacgacc
    13021 tgccaaaaca ataggaatca acttgtcttg cacctctgtt ggagttgtaa attttaactc
    13081 ctccaaggct tctctaatat agtttttaaa ttgaaatttc gtaaatgaca taacatcctc
    13141 gattctatct atcttatcaa ttataccata ttttattcca ttacagtagt ctcacttatt
    13201 taggctattt ccagtagctt ctctagtaag aaaaggctgg aattttatag ttccaacctc
    13261 ttttcagtta ttatttccag tttaacatag cattcaagcc atagtgatca ctcacttgtg
    13321 gactcttgtt accatcaaat acgacatgta aattttccac cgctaactct ttggtagtaa
    13381 agacataatc gattcgaagg ggttcagtgt tccctttcca gccatcaatt tcaggcggaa
    13441 cagtatagct accacttttc tcttgagcaa cttcaaatgc gtcttgtaag cctaatggac
    13501 tagctaaaat agcttggtaa ccttcctgac ctgctgggtt gttaaaatct ccagctagta
    13561 aaagtggctt gttcaatttt ttcaagacag cctcaaatcg tgcccattct tcttggaaac
    13621 ctttatccca ccaagagaga tgaacactgg caactgctag ctccttgcca tcgactacag
    13681 tttcagctag ggcaacacgg cgagtatgat agtctgttgg atcatccaca tctgaaacca
    13741 aaatttctct ggcttcaata ggtgttttag acaagatagc cacaccttcg tggtagcggt
    13801 tatagccgat atggttataa gcccaggtcc agtagtaatt tttcccttgc tctaacaact
    13861 tttcaaccaa gagtctaaca taatggtctt ggtgaatagg ctcagctgct ggcaaagctt
    13921 gataaaggtc attaacctcc acctctgacg aggtcatctc ctgattgatt tcttggaaac
    13981 aaatcaaatc atagtccttt tcaagaatat cttcaagcaa aatctggaat ttttcctctg
    14041 cttctttctc catccaactg tgagtattga gtgttaaaaa tttcattctt ttctcccaaa
    14101 ataagaggtt ggaatacagc ttccaacctc aagtatatta caattctact ttagcaactg
    14161 ctgttttagc tgcaagagaa cctgtttttt ctaatttaac tgatttaatt gcatcaccat
    14221 ttgtgaagac aactactgtt gaagtttcac gtcctgctgc acggatagca tccaagtcag
    14281 ctgtgacaag gagatctcct gctgcaactt tttgtccttc agcaacatga actgtaaatg
    14341 gtttaccttc aagacttact gtgtccaaac caatgtgaac caatacttca agacctgctt
    14401 ccgtcacaat accaaaagca tgttttgttg ggaagatgct tgacacagta cctgaaactg
    14461 gagatacaat gtttccattt gcaggttcta ctgcaaatcc atcacccatc attttttgag
    14521 caaatactgg atcctttact tgttccaaac caacaacttg accgtctgct actgagtaaa
    14581 cttcctcagt aagatctttg aagtgaacag tgttttgttg tgcttcagtc atttggcttg
    14641 gaagagtttc aggaatgatt tcacctgaat caaggatatc ttggatatca gatttcaaaa
    14701 tgtcagcttt tggaccgtag atagcttgaa ccccttgtcc tttcatcaca agacccatag
    14761 ctccttctgc tttccattgc tctgcatttc ctactttatc tgcatcttta acagttacac
    14821 gaagacgagt catacatgca tcaacatcaa cgatgtttac acgtccacca agaaggttga
    14881 taatgtttac agcttgagag cctgctgcaa ctttcacttc gctgctggtt tcttctgaac
    14941 cttcagcagt ttcgtagttt ccgttgcgcc ctggagttgc gtagttgaat ttttgaatca
    15001 tgaagtttgc gataaagtac atgattacag caaagagaac agttacccaa acgaagttaa
    15061 cgatatccat accaatacca gcactgattg caataggtgt acgagtcaag aactcgattg
    15121 aaccgaatga gtgcatacgt aggtttacga cgtcagccat agcgaaggca gcaccttgaa
    15181 caagtgagta aacaagatac ataggtgttg cgatgaacat gaacatgtat tcgattggtt
    15241 cagtaacccc tgtcaagaat gttgcaagag ctgttgcaat catcatacct ttgtatttat
    15301 gtttcttgtc agcatcaaca ttacggtaga tagcaacaat cacacccatc aagataccga
    15361 atgaaccgat catttgtcca actttgaaac gagctggatg tactgtatct aacaagtgtt
    15421 gatattgact agcatcagta cctttaaggt ttacaaggtc tgttacccat gcaagccata
    15481 gtgggtcttg accgaatact tgagtacctt tagctgcacc agttaaaatg tcataagtac
    15541 caccaagagc tgtgtagttc attgggatag tcaacatgtg gtgaagacca aatggcaaga
    15601 gcaaacgttc caaagtacca tacaagaatg gtgcaagaat tggagcagtt tcttgtgagt
    15661 tggcaatcca gataccgaag ttattgatac ctgtttgaac tactggccag aaagcagcaa
    15721 gtagaattgc agcgattgct gaacgaagaa taactacaaa tggtacgaaa cgtttcccgt
    15781 tgaagaatga aagtgcatca ggaagtttac ggaagttgta gtatttgttg taagcagttg
    15841 cccctacaaa acctgagata atccctacga atacccccat gttcaaggct ggagcttcaa
    15901 gaacactgat aaagtaatca gcaactttga ttgaaccacc aaagaaagta gttaccatag
    15961 catctggatt tttcaacata tcgcctgata caccaaaaat tgtaccagtg atacggttaa
    16021 tcaaaatgaa ggcaagacca gcggcgaaag caccaccagc acgttcttta gcccagcttc
    16081 ctccaatggc tagggcaaac aaaatgtgaa ggttaccgat aaccccccaa ccgatttgct
    16141 caagaattcc acctgtgatg acaagtggtg taaaggttgg gttaatcatc acgatagact
    16201 taccgattga aatcatcaaa ccagcagccg gcataaccgc gataactacc atcaaagcct
    16261 taccgaattt ttgccaaaat tcgaaagaca agacattttt gaatgtatct ttcatcattc
    16321 aaatctcctt tatttttatt taggcaaacg ttttacgaaa a
//
LOCUS       DAVLYR010000026        16020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLYR010000026 DAVLYR010000000
VERSION     DAVLYR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.026"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            30..3866
                     /gene="spuA"
                     /locus_tag="VYO63_000720"
     CDS             30..3866
                     /gene="spuA"
                     /locus_tag="VYO63_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1671681.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNKSDISSLGNANASLEKTEEKPATEPT
                     TPAASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQ
                     GLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNL
                     TGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVK
                     NPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFT
                     DLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKH
                     SNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDE
                     TYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTW
                     KQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSNDAKIDDAHKVA
                     KAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEA
                     FIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSL
                     TGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFM
                     DADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEA
                     YKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGY
                     PNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSI
                     KKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDK
                     VPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKG
                     LIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVN
                     ADEKAREFNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLR
                     VSQNGTSHESTAEEKPDSTPSKPEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQ
                     AEQPAQEAQASSVKEAVQNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGISL
                     LALLGLGFLLKNKKEN"
     gene            complement(3966..5015)
                     /locus_tag="VYO63_000721"
     CDS             complement(3966..5015)
                     /locus_tag="VYO63_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1671682.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLALLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            5072..5149
                     /locus_tag="VYO63_000722"
                     /pseudo
     CDS             5072..5149
                     /locus_tag="VYO63_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5287..7095)
                     /gene="glmS"
                     /locus_tag="VYO63_000723"
     CDS             complement(5287..7095)
                     /gene="glmS"
                     /locus_tag="VYO63_000723"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1671683.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGFDVDKPRNLAKSVTVE"
     gene            complement(7288..8667)
                     /gene="bglA"
                     /locus_tag="VYO63_000724"
     CDS             complement(7288..8667)
                     /gene="bglA"
                     /locus_tag="VYO63_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1671684.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEHYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8766..10619)
                     /locus_tag="VYO63_000725"
     CDS             complement(8766..10619)
                     /locus_tag="VYO63_000725"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1671685.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10632..11891)
                     /gene="rseP"
                     /locus_tag="VYO63_000726"
     CDS             complement(10632..11891)
                     /gene="rseP"
                     /locus_tag="VYO63_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1671686.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11913..12716)
                     /locus_tag="VYO63_000727"
     CDS             complement(11913..12716)
                     /locus_tag="VYO63_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1671687.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12725..13483)
                     /locus_tag="VYO63_000728"
     CDS             complement(12725..13483)
                     /locus_tag="VYO63_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1671688.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13714..14286)
                     /locus_tag="VYO63_000729"
     CDS             complement(13714..14286)
                     /locus_tag="VYO63_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1671689.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14267..15238)
                     /gene="ruvB"
                     /locus_tag="VYO63_000730"
     CDS             complement(14267..15238)
                     /gene="ruvB"
                     /locus_tag="VYO63_000730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1671690.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15399..15491
                     /locus_tag="VYO63_000731"
     CDS             15399..15491
                     /locus_tag="VYO63_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1671691.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         4882 a         3778 c         3035 g         4325 t
ORIGIN      
        1 tatagaaatt tattgattgg agaatcatta tgagaaaaac accatctcac actgagaaaa
       61 aaatggtcta cagcatccgt tccctcaaaa atgggactgg ttctgtcctt atcggcgcaa
      121 gccttgtctt gcttgccatg gctacaccaa ctatctcatc cgacgaaagt acaccaacca
      181 ctaacgaacc caacaacaga aatacaacca cccttgccca acctcttact gatacagcag
      241 ctggctctgg taagaacaaa agtgatattt cttcacttgg aaatgcaaac gcttccctag
      301 agaaaacaga agaaaaacct gcaacagagc caacaactcc tgctgcaagc ccagccgatc
      361 cagcaccaca aactggacaa gatcgttcaa gtgagccaac tacttctact agtccagtaa
      421 caactgaaac taaggcagaa gagcccatcg aagataacta cttccgtatc catgtcaaaa
      481 aacttcctga agaaaacaag gatgctcaag gactatggac ttgggacgat gttgaaaaac
      541 catctgaaaa ctggccaaac ggagctttgt ccttcaagga tgccaagaaa gatgactacg
      601 gctattacct agatgtcaaa ttaaagggag aacaagccaa gaaaattagc ttcctcatca
      661 acaatacagc tggaaaaaat ctaaccggcg ataaatctgt agaaaaacta gttccaaaaa
      721 tgaacgaagc ttggttagac caagattaca aggttttctc ttacgagcca cagcctgcag
      781 gaactgttcg cgtcaactac taccgcacag atggcaacta tgacaagaaa tctctctggt
      841 actggggaga tgtgaaaaat ccaagtagcg ctcaatggcc tgacggaaca gactttacgg
      901 ctacaggcaa atatggccgc tatatcgaca ttcctcttaa tgaagccgca agagaatttg
      961 gatttttatt actagatgag agcaaacaag gagacgacgt gaaaatccgt aaagaaaatt
     1021 ataagttcac agatttgaaa aatcatagcc aaattttcct aaaagacgat gatgaatcga
     1081 tttacacaaa tccatactat gtccatgata tccgtatgac aggagcccaa cacgtaggca
     1141 cttctagcat tgaaagtagc ttttcaacac ttgtcggtgc taaaaaagaa gatatcctca
     1201 aacactccaa catcactaat cacctaggaa acaaggtaac tattaccgat gttgcaatcg
     1261 atgaagctgg taagaaagtg acctacagcg gagatttctc tgacacaaaa catccttata
     1321 ctgttagcta caattccgac caattcacta ccaaaacaag ctggcgcctg aaagatgaga
     1381 catacagcta tgatggcaaa ctgggagctg acctaaaaga agaaggaaaa caagttgatt
     1441 tgaccctttg gtcaccaagt gctgataagg tttctgttgt tgtctacgac aagaatgacc
     1501 ctgacaaagt agttggaact gtcgctctcg aaaaagggga aagaggaact tggaaacaaa
     1561 ctctagacag cacaaacaaa ctcggaatca cagatttcac tggctactat tatcaatacc
     1621 aaatcgagcg tcaaggtaaa actgttcttg cactcgatcc ttacgctaaa tctcttgctg
     1681 cttggaatag caacgatgcc aagattgacg atgcccataa agtggctaaa gccgcctttg
     1741 tagatccagc taaactcgga cctcaagact tgacttatgg taagattcac aatttcaaga
     1801 ctcgtgaaga cgccgttatc tacgaagctc atgtgcgtga cttcacttca gatcctgcca
     1861 ttgcaaaaga cttgaccaaa ccatttggga cttttgaagc cttcattgaa aaactagact
     1921 atctcaaaga cttgggtgta acccatatcc agctccttcc agtcttgtct tactactttg
     1981 tcaatgaatt gaaaaaccat gaacgcttgt ctgactatgc ttcaagcaac agcaactaca
     2041 actggggata tgaccctcaa aactacttct ccttgactgg tatgtactca agcgatccta
     2101 agaatccaga aaaacgaatc gcagaattta aaaacctcat caacgaaatc cacaaacgtg
     2161 gtatgggagc tatcctagat gtcgtttata accacacagc caaagtcgat atctttgaag
     2221 acctagagcc aaactactac cactttatgg atgccgatgg cacacctcga actagctttg
     2281 gtggtggacg cttggggaca acccaccata tgaccaaacg cctcctagtt gactctatca
     2341 aatacctagt tgatacctac aaagtggatg gcttccgctt cgatatgatg ggagaccatg
     2401 acgccgcttc tatcgaagaa gcttacaagg ctgcacgcgc cctcaatcca aatctaatca
     2461 tgttaggcga aggttggaga acctatgccg gtgatgaaaa catgcctact aaagctgctg
     2521 accaagattg gatgaaacat accgatactg tcgctgtctt ttcagatgac atccgtaaca
     2581 acctcaaatc tggttatcca aacgaaggtc aacctgcctt tatcacaggt ggcaagcgtg
     2641 atgtcaacac catctttaaa aatctcattg ctcaaccaac taactttgaa gctgacagcc
     2701 ctggagatgt catccaatac atcgcagccc atgataactt gaccctcttt gacatcattg
     2761 cccagtctat caaaaaagac ccaagcaagg ctgagaacta tgctgaaatc caccgtcgtt
     2821 tacgacttgg aaatctcatg gtcttgacag ctcaaggaac tccatttatc cactccggtc
     2881 aggaatatgg acgtactaaa caattccgtg acccagccta caagactcca gtagcagagg
     2941 ataaggttcc aaacaaatct cacttgttgc gtgataagga cggcaaccca tttgactatc
     3001 cttacttcat ccatgattct tacgattcta gcgacgcagt caacaagttt gactggacta
     3061 aggctacaga tggtaaagct tatcctgaaa atgtcaagag ccgtgactat atgaaaggtt
     3121 tgattgccct tcgtcaatct acagatgcct tccgacttaa gagtctccaa gatatcaaag
     3181 accgtgtcca cctcatcact gtcccaggcc aaaatggtgt ggaaaaagag gatgtagtga
     3241 ttggctacca aatcactgct ccaaacggcg atatctacgc agtctttgtc aatgcggatg
     3301 aaaaagctcg cgaatttaat ttgggaactg cctttgcaca tctaagaaat gcggaagttt
     3361 tggcagatga aaaccaagca ggaccagtcg gaattgccaa cccgaaagga cttgaatgga
     3421 ctgaaaaagg cttgaaattg aatgccctta cagctactgt tcttcgagtc tctcaaaatg
     3481 gaactagcca tgagtcaact gcagaagaga aaccagactc aaccccttcc aagcctgaac
     3541 atcaagaccc agctccagaa gctagacctg attctactaa accagatgcc aaagtagctg
     3601 atgcggaaaa taaacctagc caagctacag ctgattcaca agctgaacaa ccagcacaag
     3661 aagcacaagc atcatctgta aaagaagcgg ttcaaaacga atcggtagaa aactctagca
     3721 agaaaaatat acctgcaacc ccagatagac aagctgaact tccaaataca ggaatcaaaa
     3781 acgaaaacaa actcctattt gcaggaatca gcctccttgc gctccttggt ctcggtttct
     3841 tactaaaaaa taaaaaagag aactaaacta gccctcctat agaaaaatcc cccaagcatt
     3901 atagctcggg ggattaattt tcgtacaata tttgttgtcc taataaactt gattaggatt
     3961 ttttattaag cctctttcat agcaaaataa gctcgtactt tgggtgcaac ttgtgttccg
     4021 aagagttcaa tagctctcag aacctggtca tgaggcatag aaccaagcgg tagatgaagc
     4081 atgaagcggt ccaatcctaa atcctctatc atgcgaatca atttttcggc cacctgatct
     4141 ggattgccaa caaacatggc gccatttggc cctacctgct ccaaatattg ctcataacgc
     4201 aattcctgcc agtgcggacg gtctttggaa atagcatcca ccacttgctt agtcggatgg
     4261 aaataatctt tcaccgcctg ctcaccatct tccgcaatcc acccccaaga atgggctccc
     4321 actttcaagt ctttgtcagc atgacccgct tcgcttccaa tctcacgata agcctgaatc
     4381 aactttttaa aataacgtgg attaccacca ataatagcat atacaatcgg tagaccagcc
     4441 tgagcaatct tcactgttga ttcgacatga ccacctgtag ctatccacaa gggcaatttg
     4501 tcctgaactg gacgaggata aacttcttta ccagcaatcg tttgagtcaa tcgaccttgc
     4561 cagtctaact tggtcttttc attgactaac tgaagcaagg ctaatttctc atcaaaaaga
     4621 gagtcgtagt ctttcaagtc ataaccaaac agagggaaag attccgtgaa agagcccctt
     4681 ccagccataa tctccgctcg tccatttgac aaagcatcga tagtggcata ctgttggaac
     4741 aaacgaatcg ggtccatgct tgacagaatg ctgactgcac tggtcaaacg gattttcttg
     4801 gtattgactg ccccagcggc cagaacaatc tctggggctg atactgcaaa atccgcccga
     4861 tggtgctcac caatcccata tacatccaaa ccaaccttgt cagccagctc aatctctgcc
     4921 accaactggc gaatgcgttc agcatgactg taagtttgtc cagtcccttc aagctccgtt
     4981 atttccccaa atgttgaaat tcccaattct accattgtga ttctccttat ctatctctgt
     5041 acttcaattt gaaaaattat tctaatactc aatgaaaatc aaagagcaaa ctaggaagct
     5101 agctgcaggc tgctcaaaac agtgttttga ggttgtggat agaactgacg aagtcagtaa
     5161 ccatacctac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtataacac
     5221 gaatcttgag tacaagcaac caatttgctc acaagaaaaa ggcctagata actagacttt
     5281 tttagcttat tctaccgtta ctgacttggc aaggttacgt ggtttgtcca catcgaagcc
     5341 acggtggagg gttgcaaagt aagcgactaa ttgcgttggt acgaccattg aaattggtga
     5401 gaggtatgga tgtacggtcg taaggacgat atcgtcggta tctttggcta cattctcttc
     5461 tgcgatagtg aggactttgg caccacgggc tgcgacctct tggatatttc cacgagtatg
     5521 gttggcaaga actggatctg acaagagaga caagacaggc gttccttctt caatcaaggc
     5581 aatggttccg tgcttgagtt ctcctgccgc aaaaccttca cactggatat aagaaatctc
     5641 tttgagtttg agacttgctt ccatggctac gtagtaatct tgaccacgtc cgatgtaaaa
     5701 ggcgttacga gttgtttcaa gaagttcacg aaccttggct tcaatggttt ctttctctga
     5761 aagagttgat tcgatagact gagctacgat tgacaattca tgaaccaggt caaaggcttg
     5821 cgctttagca ttaccatttg cttctccgac tgcttttgca aggaaggcaa gggctgcgat
     5881 ttgcgctgta taggctttag ttgatgccac ggcaatttca ggacctgcgt gaaggagcat
     5941 ggtatagttg gcttcacgtg agagggttga acctggaacg tttgtcactg ttaagcttgg
     6001 aattcccatt tcattagcct tgaccaaaac ttgacgacta tccgctgttt caccagattg
     6061 gctgataaag atgaagagtg gtttcttgct gagaagtggc ataccgtagc cccactcaga
     6121 tgagattcca agttcaactg gtgtatctgt caattcttcc aacattttct tagaagcaaa
     6181 tcctgcatgg taagatgttc cagctgcaag gatgtagatg cggtctgcgt cttgaacagc
     6241 cttaatgata gcaggatcaa tcactacttg accagcatca tccgtgtagg cttgaatgag
     6301 tttacgcata acagttggtt gctcatcaat ttccttaagc atgtagtaag gataagttcc
     6361 cttaccgata tctgacaagt caagttccgc agtatagcta gcacgttcac gactgttacc
     6421 atcatagtct tgaacttcca cgctatcagc cttgacgatt accaactctt ggtcatgaat
     6481 ttccatgtat tggttggttt cacgaatcat agccatagca tctgagcaga ccatattgta
     6541 gccttcccca agaccaatca aaagtggaga tttgtttttc gctacataga tgacatctgg
     6601 attttcagag tcaatcaagg caaaggcata tgaaccacgg ataatatgaa gagctttttt
     6661 gaaggcttca agaactgaga gcccttcttc ttccgcaaat tttccaatca aatgaacggc
     6721 tatttcagta tctgtctgcc ccttgaagtg gtgacctgca aggtattctt ccttgatttc
     6781 aagatagttc tcaatcaccc cattatgcac caagacaaaa cgttctgtct cagagcggtg
     6841 tgggtgagca ttgtcctcag ttggttttcc gtgagtagcc caacgagtat gtccgatacc
     6901 agttgttccc tcaacaccag ctgtcttggc agacaattct gcaatacgac caaccgcctt
     6961 caccaaatgg ttatcagcac catctaggac aaaaattccc gcagaatcat agccacggta
     7021 ttcaagcttt tcaagccctt gaatcaaaat atcagttgca tttgtgtttc caacaacacc
     7081 aacaattcca cacatagtat atacgacaca ggcaagctgt gctttctcct taaaattggt
     7141 atagtctaat tcatctttta tagaatcagt aaaaacagta tatacttgtt tctttcactt
     7201 gtcaagagta aaaattggta tagttcaaat taagctcctg taagcataaa aactctgacc
     7261 gattgggata atcagtcaga gtccttttta aaatccatta ttatcgctta attctttgaa
     7321 ccagtggcct gatttcttca gacgacgttc ttgcgtttcc aagtctaatt cgaccaaacc
     7381 atagcgattt ttatagctgt tgagccatga ccagcagtca ataaaggtcc aaatcaagta
     7441 gcccttacag ttggcaccat cttcaatggc acggtgaagt tcacgaagat gaccttttac
     7501 aaagtcaata cggtaatcat cttgaatcat tccatcttga cggaattttt cttccccttc
     7561 aacacccata ccattctcag tcaacatcca ctcaatattg ccataatttt ccttgatatt
     7621 ttgggcgatg tcataaatcc cttgctcata aatctcccaa ccacggtgag aattgatttt
     7681 acgtccaggc atcacataat gctcgtaaaa atgttctggt aagagtggac tctctggatg
     7741 cttagcaaat cgaggagcca taacacgcaa aggttgatag tagttcacac caaggaagtc
     7801 caccgtatta tcacgaatga gttccaactc ttcctctgta gcatcaggta aaagaccgtg
     7861 ttcatgcaag atttctacca actcctgtgg ataagtcccc aagacagatg gatctaagaa
     7921 agattgggcc tgaaaaaggg ccgcaatacg agctgccttg acatcagcag gatgctggct
     7981 acgtggataa gccggtgtca agttgaggac aatcccaatc ttggaatcag gcaaaagttc
     8041 atggcaagcc ttaacagccc ggctgctggc caattgtgta tgataggcta ccttaacagc
     8101 tgcctctgca tccaccttat gtggataatg ggcatcataa aaataaccaa attctacagg
     8161 aacgatgggc tcgttaaagg taatccattg atccactaaa tctccataag tctcaaaaca
     8221 aaaacgagca tagtcttcat aggctgaaac tgtcgcctta ttttcccaac catcaccatc
     8281 ctcttgaagg gcaaaaggta aatcaaaatg atagagattg actaacagac gaattccttt
     8341 agccttaata gcctcaaaga ccttacgata aaaatccaca ccttgagggt tgacttttcc
     8401 acagccttgt ggaaaaatcc gtgaccactg aatagaagtc cgaaaggctg tgtgaccagt
     8461 ctctaacaaa agctcaatat cccgctccca attttcataa aaagtcgatg tcttatctga
     8521 accaatccca ttatagtaac gatttggctc cacttggaac cagtaatccc agagattgtc
     8581 tcccttaccg tcaccagcta cacgtccttc tgtctgcggt ccagaagtag aggatcccca
     8641 gacaaaatcc tttggaaatc ttagcataca tttacctctt tatctactca tttctcccat
     8701 tatacagaaa aaacaaggta aaaactagtt acattttttc cttgtttttc ttctgattat
     8761 agtttttatt tcttacttag gatttcaagc gtttcaagca cgttatctgc atgaacctca
     8821 atggtgtcac cagttgcctt gatcttaact tctacaatgc catcggccgc ttttttacca
     8881 acagtgatac ggattggaag accaatcaag tcactatcgc taaatttaac accgacacgt
     8941 tcgttacggt catctgtcaa gacttcataa ccagctccca tcaagcttgc ttcaagtttt
     9001 tctgtcaagg cttgcgcttc ttcatccttg acattaacag taatcaaatg cacatcaaat
     9061 ggtgccaatt ctttagggaa attgattccc caagcgtaac ggtattcacc ttttggcgtt
     9121 ttgttaacaa agaggcgagc gtgttgctcc atcactgctg aaagaagacg gctgacaccg
     9181 ataccgtaac atcccatgat gattggcaca gcacgaccat tttcatccaa gacatctgct
     9241 cccatgcttg ctgaatagcg agttccgagt ttgaaaatat gaccgatctc aataccacgc
     9301 gcaaaattaa ggacaccttg tccatctggg gaaatttcac cctcacgaac ttcacggata
     9361 tccacatatt ctgaagtaaa atcacggcct gggttcacac cagtcaagtg gtagccatct
     9421 tcgttagcac cgacaactgc attgcgaaca tcttgcacct tacgatctgc aataatttta
     9481 atattctctg gcaaaccaac tggtccaagt gaaccaaatc ctgcttgaac aacattcgcc
     9541 acttcttctt cgctagcaac gtcaaagaaa tctgctccta agtggttttt caacttgact
     9601 tcgttgagtt ggtcatttcc aactagaagg gctgcaacaa gctcaccatc tgcaatgtag
     9661 aagagggttt taatcgtttg ttcttctgga acattgagga aagctgcaac ttcatctatt
     9721 gatttaacat ctggcgttgc aacacgagta acttcttctt cagcgacaac acggttgctt
     9781 ggtttgtact cgtttgttgc catttctaag ttagctgcat agctagactc acttgagtaa
     9841 gcaatggtat cttcaccaga gactatccat ttgagcaatt ctgccttgat ttcttcttgc
     9901 acttctgcag gaatttcgtc aaatgaggca actgacttgt ccaagacaac ccagcggtca
     9961 aggtctgtac gagcagatgt aatggccata aattcttggc tatccttacc acccatggct
    10021 ccaccgtcac caataatagc cttgaagtct aaaccactac gagtaaaaat acgctcatag
    10081 gctgctttgt actcatcata aacactatcc aaactatcat agttagcgtg gaaactataa
    10141 gcatccttca tgataaactc acgtgtacga agaagtccat tacgtgggcg tttttcatca
    10201 cgatacttgg gctgaatttg ataaaggttg agtggcaatt gcttgtaaga tttaacagaa
    10261 tcacggacaa tagctgtaaa ggtttcttcg tgagttggac ctaagataaa gtctgatttt
    10321 tcacggtttt ttagtttgta aaggtcttca ccataggttt cgtaacgacc tgattcacgc
    10381 cacaattctg cactaagaag ggctggagcc aacatctcaa cagcaccaat cttttcgaat
    10441 tcttggcgca tgatgttttt agctttttca atcacacgat tggcaagtgg tagataagaa
    10501 taaacacctg ctgaaacttg gcgaacataa ccagcacgca acataagagc atggctgata
    10561 acttgagcat cgcttggcat ttcgcgaagc gttgggatag gcattttact ttgtttcata
    10621 atattcctcg attatctaaa aaagagtcgc ataatgtcat tccaagtcac agcaatcatc
    10681 aagacaacca tgatgaccac tccggccaag gtgacatagg tttcaatttc ttgtttcaat
    10741 ggtttgcggc ggatggcttc taggatattg agcacaatct taccaccatc caaggctgga
    10801 atcggaataa gattaaaaat cccaatattg atggaaatca ttgccaagaa gtacaagata
    10861 ttttcaattc catttttagc agcatcacta cttgccttaa agatagcaac aggtccaccc
    10921 aacttgttca aatctggttg gaaaatcaga tttttcagag ctgagagaat tcggagagct
    10981 gagtcagcag cagttgtaaa accacctaca aacatggata gaaaatctga cttaaccccc
    11041 ggttgaacac ctagaaggta acgaccttga ctatctttgg gtgtaacagt gacttgtttg
    11101 tcactcccct tttcagaaat agtcacatcc aaagtcggtg ccgtcttatc tttggtttct
    11161 gtttccacag cttggatcaa gctttcccag ttgctaacct catgtgagcc aatcttggta
    11221 atttgtgccg tttctggtac tcctaccttg gccaaggcac cttggggcat gatatggaac
    11281 tgattggtat caacatctct gacaccaccc tgcataaaga ttaaaaccca aaaaacaacg
    11341 acacctaaga taaaattgtt cataggacct gcaaaattgg taatcagttt gccccagata
    11401 gtcgcatttt gatattgaac atctaaaggt gcaatccgaa cctcagtacc atctgcttcc
    11461 acaaccgttg catcgtgatc cactgcaaat gttttttctt cttccagaac caatcctttg
    11521 ataaagagct tgtcttcaaa atcaaactgg gtcacctgca tagggagggc tgtttgatcc
    11581 aattttttac ctgagagatt gatgcgttta accttaccat catcagcaag tgtcaaacta
    11641 acaggcgttc ctgtcttgat ttcagttgta tcatcacccc aaccggccat gcggacatag
    11701 ccacccagag gcaagattcg aatggtatag gccgttccat ccttgccaat gtgagcaaaa
    11761 attttaggtc ccataccgat ggcaaattca cgtactaaaa tccctgattt cttggcaaag
    11821 tagaagtgcc cgaactcgtg caccactaca ataatcccga aaaccagaat aaaggttaaa
    11881 attccgagca tagcgtttcc tccgtctttt gattaaaaga gtccaaataa gtgcatgatt
    11941 ggaaatacaa gcaacatact atcgaaacga tccaaaacac caccatgtcc agggataaat
    12001 ttcccagaat ccttaacacc aaaatgacgt ttgatcgaac tttctagtaa atcaccaaat
    12061 tgtccagcaa tgctaaagaa aatagcaaag actgacatct tgtaaattcc atatggaaga
    12121 gcaactgtac tgtcaactat cataaagata atggttacta aaattgctcc taaaatacca
    12181 cccaaggcac cctcaagggt tttattaggc gatacccttg gtgctaactt tcgtttccca
    12241 tagttcatcc caacaagata ggcaccactg tctgtcgccc agacgataca caaggctaag
    12301 agagccttgt ccaaacctgc aacacgagca tctagtaaag cattaaatcc aaagcccacg
    12361 tagaagctca tagcaagagg gaaaaccgca tcctcaatcg tataagactt gctaaaaacg
    12421 gtcgttccta acatgattga aatcaaaaca ctataggcaa ccacattccc atcaactggc
    12481 aaaaaagtca ggtaattctc caagggaatg gtcaatgcaa aggttgcaaa gagggtcaag
    12541 aggccctcca tcgtcatggt ctctagacct ctcatcttca aaagttcatg catggctagc
    12601 atggctatga ttccgattgc tatctgaagc aagaggcccc caatcattaa aattggtagg
    12661 aaaatagcca gggcaatccc tgcaaacaag gttcttttct gtaaatcctg ggtcatattt
    12721 cctcctaaac tcctccaaat cggcgatgac gacgattata ggcaagaata gcttcctgca
    12781 aggccgcttc gtcaaaatca ggccataagg tgtccgtaaa ataaagctca ctataggctc
    12841 cctgccatgg aaggaaattg ctcaaacgca attctccact agtacggata atcaagtctg
    12901 ggtctcgtaa gtccttaggc aaatgctggg taaagagata gttaccaatc aattcctctg
    12961 tgatgtcacc tggattgatt ttggcatcta aaacatcctg ggaaatcaac ttaagcgcct
    13021 gtgtaatctc agcacgtcca ccatagttaa gagcaaaatt aagaatcaat cctgtgttgt
    13081 tcttagtcaa ttcctcagcc ttggttaaag cttcaaaggt ttgcttaggc aggcggtctg
    13141 tctccccaat catttgaatc ttaacattat tcgcatgtag ttccgggaca taattatcat
    13201 aaaactctac tggcaagttc atgataaact tgacttcctg atctggacgg gtccagtttt
    13261 ccgtagaaaa agcatagacc gtaataacct tgacgcccag tttgttggct gccttggtca
    13321 cggtttgcaa tgcttccatg cccgccttat gtccaaaaac tcgcggttgc atacgttttt
    13381 tagcccaacg gccattgcca tccatgatga tgccgatatg agcaggaacc tgtgtcggaa
    13441 cctctacttc cacagcctta tctttcttaa aaaatccaaa catgatctta ttcctattca
    13501 aaaatctatc gttttattat accatatttc cccattttct tctatcacta agctatttat
    13561 tctcaggcac caagcccatt tttcaaaaaa ataagccgcc tgattgggcg actttatttt
    13621 tatagggaga ttattatgaa aaagttttag gagtttaagt taaggtcttc ttaacttatg
    13681 aacttagtgt acactcccta gcttaaagtt tccttaagta tttttaaaaa tcaaattttt
    13741 ccatttctcc tgccaatttt tcttggataa acgtgtttga tagagttcca ttcggtcttc
    13801 attttctaag aaatgaggag ttggacgaac ttgaaaattc aaaatatcct ccaaaccata
    13861 aggtgcatag agttcaaaat ctgattcttc attcaagcgc agtccaactg tcgtacaccg
    13921 ttctggatac ttactcatag catcacgaga actggtatag gaagcagtgt gaggactgtg
    13981 ctgatgcata tagacctgat ttttcaattc ccactggtac tgaggaaaat cctctcgcag
    14041 ctttttctcc agtaataagg tttcctcata agaaaaatct ggatcaaaga aaatcacatc
    14101 tatatctgtt tcatgatcaa aaggggattt gtctgacaaa agattccaaa tgaaatttct
    14161 gacagaacct gctgccaacc acgagtcttt cagaccaagg tctcggatga tcgtcagaat
    14221 ggccatcata tctggatttt ctctaaaagc ctctaagatt tcttgcttat ttttcactgt
    14281 attcataacc taagtgctca tatgccttag cagtcgccac ccgtccagac cgtgtccgca
    14341 tgataaaacc tttttgaatc aagtaaggct catacatatc ttcaactgtc tcacgctctt
    14401 cggcgatatt cacagaaaga gttcctagac caacaggtcc tccactgtac atctcaatca
    14461 tggtacgaag gattttttga tccacatagt ccaaaccttc atggtcaaca tccagcatag
    14521 tcaaagcctt atcggtaata atatcatcaa ttaccccatt ccccattatc tgggcaaaat
    14581 cgcgcacgcg cttgaggaga cgattggcaa tacgaggggt cccacgacta cgtagggcca
    14641 actcagatgc tgcctcatga gtgatttcca tctcaaaaat atctgccgtc cgctcgacaa
    14701 tttctgtcaa gtcagcatgg gcataatact ccatatggcc tgtaatccca aaacgtgccc
    14761 gtagcggatt ggagagcata ccagcccgag tcgtcgcacc aatcaaggta aaaggtggta
    14821 actccaaatg aacactacga ctaccctcac cagccccaat cataatatct atgtagaagt
    14881 cctccatagc actataaagc acctcttcca ctgacattgg caaacgatgg atctcatcaa
    14941 taaaaagtac atccccaggc tctaactcat tcaaaatagc taccagatct ccggcttttt
    15001 caatgactgg acccgacgtc tgcttaagat tgactcccag ttcgttggca ataacaaagg
    15061 ccatggtcgt tttcccgaga cctggaggcc caaataagag cacatgatcc agcgcttcat
    15121 cccgcatttt ggcagcttca ataaagattt gtagctggtc cttgacctta tcctgtccga
    15181 tatattcacg taaatactga ggacggagcg tgcgttctac taactcctca tcccccatta
    15241 tctcattatc taaaattcta ctcatggctc tattatatca aaaaaccaag ccacaaacaa
    15301 aaaagccacc tgattgggtg actcctaagt ttagcactta tgtggtataa tattctacga
    15361 cactgctaca ccgcctacga aaggaggtga gatagcccat gatggaatta gtactcaaaa
    15421 ctatcatcgg accaattgtg gtcggtgtcg ttcttcgtat agtcgataaa tggctaaaca
    15481 aggacaaata gtgtcaaaaa agaccccaag cttatttggt cgtgagcttg gggtcttttc
    15541 tagcctatga tatagaacta gtactcaatt cctttttatt atctcatagt tcacgaattt
    15601 tgtcaaaggt ttacattttc ttcaaccgct gtacgacaag acggttaaga ttaagagaac
    15661 gttagggatt ctatcaattt catagaaatt ttgatttcgt aaacgaagag acaatcttac
    15721 atgtcacttc tcatttaata cgccactact agacaagcaa aatcattatt acagtagttc
    15781 cagttcttca attaacagtc acttacaatc aaattgagtt tgaactagct gaagcgacca
    15841 cagacctatt tcttagttat attcgctaaa aaagtccccg ccaattcccc gaccaaaatc
    15901 cgaaaaatac cgaaaaatat cgaaaaatta tttttagaat agtccccaaa agcctgaaat
    15961 agagctaaaa aactccacct gattgggtgg agttaaggga gattattatg aaaaagaaaa
//
LOCUS       DAVLYR010000027        15847 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLYR010000027 DAVLYR010000000
VERSION     DAVLYR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.027"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            282..695
                     /gene="rpsL"
                     /locus_tag="VYO63_000732"
     CDS             282..695
                     /gene="rpsL"
                     /locus_tag="VYO63_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1671692.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            715..1185
                     /gene="rpsG"
                     /locus_tag="VYO63_000733"
     CDS             715..1185
                     /gene="rpsG"
                     /locus_tag="VYO63_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1671693.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1610..3691
                     /gene="fusA"
                     /locus_tag="VYO63_000734"
     CDS             1610..3691
                     /gene="fusA"
                     /locus_tag="VYO63_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1671694.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3795..8186
                     /locus_tag="VYO63_000735"
     CDS             3795..8186
                     /locus_tag="VYO63_000735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1671695.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREVFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8288..8551
                     /locus_tag="VYO63_000736"
     CDS             8288..8551
                     /locus_tag="VYO63_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1671696.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8544..8822
                     /locus_tag="VYO63_000737"
     CDS             8544..8822
                     /locus_tag="VYO63_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1671697.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8843..8995
                     /locus_tag="VYO63_000738"
     CDS             8843..8995
                     /locus_tag="VYO63_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671698.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            9017..10258
                     /locus_tag="VYO63_000739"
     CDS             9017..10258
                     /locus_tag="VYO63_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1671699.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10268..10498
                     /locus_tag="VYO63_000740"
     CDS             10268..10498
                     /locus_tag="VYO63_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1671700.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            10721..11443
                     /locus_tag="VYO63_000741"
     CDS             10721..11443
                     /locus_tag="VYO63_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1671701.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            11660..12994
                     /gene="pepC"
                     /locus_tag="VYO63_000742"
     CDS             11660..12994
                     /gene="pepC"
                     /locus_tag="VYO63_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1671702.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQTKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(13050..13961)
                     /locus_tag="VYO63_000743"
     CDS             complement(13050..13961)
                     /locus_tag="VYO63_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1671703.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13985..14788)
                     /locus_tag="VYO63_000744"
     CDS             complement(13985..14788)
                     /locus_tag="VYO63_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1671704.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(14816..15805)
                     /gene="manX"
                     /locus_tag="VYO63_000745"
     CDS             complement(14816..15805)
                     /gene="manX"
                     /locus_tag="VYO63_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1671705.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         4706 a         3119 c         3688 g         4334 t
ORIGIN      
        1 gacggagata aaaatgtcac ataagccaag ccgtattatt aggaagatga tgtgttccca
       61 gctattttta ttcagaaatt gatctctaac attaccaata aaaaagaaaa atatcttgac
      121 aaacaaggga aaattttgtt acaataatag acggtacttt ttacttttgg tctctcaaga
      181 gtgtacaggg acgtgctgac aaatgttgca aaagtacaca cagatgatat ctgtcaccaa
      241 gtgtatcatc accaaaaata aaaaaacaca ggagaatgta gatgcctaca attaaccaat
      301 tggttcgcaa accgcgtaaa tcaaaagtag aaaaatctaa atcaccagct ttgaacgttg
      361 gttacaatag tcataaaaaa gttcaaacaa acgtttcttc accacaaaaa cgtggtgttg
      421 caactcgtgt tggaacaatg acacctaaaa aacctaactc agcccttcgt aaattcgctc
      481 gtgtacgttt gagcaacctt atcgaagtta ctgcctacat cccaggtatc ggacacaact
      541 tgcaagagca cagcgtggtg cttcttcgcg gtggacgtgt aaaagacctt ccaggggtac
      601 gttaccatat cgtccgtggt gcacttgata ctgcaggtgt taacgatcgt aaacaaggtc
      661 gttctaaata cggtactaaa cgtccaaaag cataaggaaa ggggataaag agaaatgagt
      721 cgtaaaaata gagctccaaa acgtgacgta ttgccagatc cgctatacaa ttcacaacta
      781 gttactcgtc ttatcaaccg cgttatgctt gatggtaaac gtggtactgc tgcttcaatc
      841 gtttacggtg cttttgagca aatcaaagaa gctactggca acgatgcact tgaagtattt
      901 gaaacagcta tggaaaacat catgcctgta cttgaagtac gtgcacgtcg tgttggtggt
      961 tctaactacc aagtcccagt tgaagttcgt ccagaacgtc gtacaacact tggacttcgt
     1021 tggttggtaa caatcgctcg tcttcgtggt gaacacacaa tgcaagaccg tcttgcaaaa
     1081 gaaatcttgg atgctgctaa caacactggt gcagctgtta agaaacgtga agatactcac
     1141 cgtatggctg aagctaaccg tgcattcgca cacttccgtt ggtaagatag gatgcgaaag
     1201 cgttaagaaa gtcccagaga aaatagggaa tcgaagcagg ttgcggttgc aaccaatgag
     1261 attcatcttt ttctccagac ttttagcttg agctcaacta aatcatgatg ctaggcacgg
     1321 taaggatgca aggtgaaaat aggaaactga cgcagtattc aacgaataca aggagtttta
     1381 tctttttcac gcagcatccc gttccagctc acatcggcta actaacttta gcccgggttc
     1441 aaattagcta aatcgattag tattagctat aactcagctt accatctcgt aagttgaaac
     1501 caacaatagc atgaaaacat tgagaacggg taggtcctgc ctatccgttt ttattaaaat
     1561 cgtgttataa tagaatagaa atcaaaaata aataggagaa acaaacctca tggcacgcga
     1621 attttcactt gaaaaaactc gtaatatcgg tatcatggct cacgtcgatg ccggtaaaac
     1681 aacaactact gagcgtattc tttactacac tggtaaaatc cacaaaatcg gtgaaactca
     1741 cgaaggtgcg tcacaaatgg actggatgga gcaagagcaa gaacgtggta tcacgatcac
     1801 atctgctgcg acaacagctc aatggaacaa ccaccgcgta aacatcatcg acacaccagg
     1861 acacgtggac ttcacaatcg aagtacaacg ttctcttcgt gtattggatg gtgcggttac
     1921 cgttcttgac tcacaatcag gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac
     1981 tgagtacgga gttccacgta tcgtatttgc caacaaaatg gacaaaatcg gtgctgactt
     2041 cctttactct gtaagcacac ttcacgatcg tcttcaagca aatgcacacc caatccaatt
     2101 gccaatcggt tctgaagatg acttccgtgg tatcattgac ttgatcaaga tgaaagctga
     2161 aatctatact aacgaccttg gtacggatat ccttgaagaa gacatcccag ctgaatacct
     2221 tgaccaagct caagaatacc gtgaaaaatt gattgaagca gttgctgaaa ctgacgaaga
     2281 attgatgatg aaatacctcg aaggtgaaga aatcactaac gaagaattga aagctggtat
     2341 ccgtaaagcg actatcaacg ttgaattctt cccagtattg tgtggttcag ccttcaaaaa
     2401 caaaggtgtt caattgatgc ttgatgcggt tatcgactac cttccaagtc cacttgacat
     2461 cccagcaatc aaaggtatta acccagatac agacgctgaa gaaactcgtc cagcatctga
     2521 cgaagagcca tttgcagctc ttgccttcaa gatcatgact gacccattcg taggtcgttt
     2581 gacattcttc cgtgtttact caggtgttct tcaatcaggt tcatacgtat tgaatacttc
     2641 taaaggtaaa cgtgaacgta tcggacgtat ccttcaaatg cacgctaaca gccgtcaaga
     2701 aatcgacact gtttactcag gtgatatcgc tgctgccgtt ggtttgaaag atactacaac
     2761 tggtgactca ttgacagatg aaaaatctaa aatcatcctt gagtcaatca acgttccaga
     2821 accagttatc caattgatgg ttgagccaaa atctaaagct gaccaagaca agatgggtat
     2881 cgcccttcaa aaattggctg aagaagatcc aacattccgc gttgaaacaa acgttgaaac
     2941 tggtgaaaca gttatctcag gtatgggtga acttcacctt gacgtccttg ttgatcgtat
     3001 gcgtcgtgag ttcaaagttg aagcgaacgt aggtgcgcct caagtatctt accgtgaaac
     3061 attccgcgct tctactcaag cacgtggatt cttcaaacgt cagtctggtg gtaaaggtca
     3121 attcggtgat gtgtggattg aatttactcc aaacgaagaa ggtaaaggat tcgaattcga
     3181 aaacgcaatc gtcggtggtg tggttcctcg tgaatttatc ccagcggttg aaaaaggttt
     3241 ggtagaatct atggctaacg gtgttcttgc aggttaccca atggttgacg ttaaagctaa
     3301 gctttatgat ggttcatatc acgatgtcga ctcatctgaa actgccttca agattgcggc
     3361 ttcactttcc cttaaagaag ctgctaaatc agcacaacca gctatccttg aaccaatgat
     3421 gcttgtaaca atcactgttc cagaagaaaa ccttggtgat gttatgggtc acgtaactgc
     3481 tcgtcgtgga cgtgtagatg gtatggaagc acacggtaac agccaaatcg ttcgtgctta
     3541 cgttccactt gctgaaatgt tcggttacgc aacagttctt cgttctgcat ctcaaggacg
     3601 tggtacattc atgatggtat ttgaccacta cgaagatgta cctaagtcag tacaagaaga
     3661 aattattaag aaaaataaag gtgaagacta atccgtcctc actctagaag gaagtcactt
     3721 agtggcttcc ttttgtcttt agaaaatacc tctaaatatg gtaaaatagt agaagaataa
     3781 tgtgaggaaa atgaatgtca aatagttttg aaattttgat gaatcaattg gggatgcctg
     3841 ctgaaatgag acaggctcct gctttagcac aggccaatat tgagcgagtt gtggttcata
     3901 aaattagtaa ggtatgggag tttcatttcg tattttctaa tattttaccg attgaaatct
     3961 ttttagaatt aaagaaaggt ttgagcgaag aattttctaa gacaggcaat aaagctgttt
     4021 ttgaaattaa ggctcggtct caagaatttt caaatcagct cttgcagtcc tactataggg
     4081 aggttttctc tgaaggtcca tgtgctagtc aaggttttaa gtccctttat caaaatttgc
     4141 aagttcgtgc tgagggtaat cagctattta ttgaaggatc tgaagcgatt gataaggaac
     4201 attttaagaa gaatcatctt cctaatttag ccaaacaact tgaaaagttt ggttttccaa
     4261 cttttaactg tcaagtcgag aagaatgatg tcttgaccca agagcaggaa gaggcctttc
     4321 atgctgaaaa tgagcagatt gttcaagctg ccaatgagga agcgctccgt gctatggaac
     4381 aactggagca gatggcacct cctccagcgg aagagaaacc agtctttgat tttcaagcga
     4441 aaaaagctgc agctaaaccc aagctggata aggcggagat tactcctatg atcgaagtga
     4501 cgacagagga aaatcgtctg gtatttgaag gggttgtttt tgatgtggag caaaaagtga
     4561 ctagaacagg tcgtgtttta atcaacttta aaatgacgga ctatacttca agtttttcta
     4621 tgcaaaagtg ggttaaaaac gaggaagagg cccagaagtt tgacctcatc aagaagaatt
     4681 cttggctccg agttcgaggg aatgtggaga tgaataattt cacacgcgat ttgactatga
     4741 acgtacagga tctgcaggaa gttgttcact atgagcggaa ggatttgatg ccagaaggtg
     4801 agcgtcgggt tgagtttcat gctcatacta acatgtcgac tatggatgct ttgccagagg
     4861 tcgaagagat tgttgcaaca gctgctaagt ggggacacaa ggcggttgct atcacggacc
     4921 atgggaatgt ccagtccttt ccacatggct ataaggcggc taagaaagcg ggaatccagc
     4981 tgatctatgg aatggaagcc aatatcgtgg aggaccgtgt ccctatcgtc tataacgaag
     5041 tggagatgga cttgtcagaa gcaacctacg tggtctttga cgtggaaacg acgggacttt
     5101 cagctatcta taatgacttg attcaggttg cggcctctaa gatgtacaag gggaatgtta
     5161 ttgctgaatt tgatgaattt atcaatcctg ggcatccctt gtcagctttt actacagagt
     5221 taactggaat tacagatgat catgtcaaaa atgccaaacc actagaacaa gttttgcaag
     5281 aattccaaga attttgcaag gatacggtcc tagttgccca caatgctacc tttgacgttg
     5341 gctttatgaa tgctaattat gagcgtcatg gtcttccaaa gattagtcag ccagttattg
     5401 atacgctgga gtttgctaga aacctctatc ctgagtataa acgccatggt ttggggcctt
     5461 tgaccaagcg ttttggtgtg gccttggaac atcatcacat ggccaactac gatgcggaag
     5521 cgactggtcg tctgcttttc atctttatca aagaggtagc agaaaaacat ggtgtgaccg
     5581 atttagctag actcaacatt gatctaatca gtccagattc ttacaaaaaa gctcggatca
     5641 agcatgcgac catctatgtc aagaatcagg taggtctaaa aaatatcttt aagctggttt
     5701 ccttgtctaa taccaagtat tttgaaggag tgccacggat tccgagaacg gttctagatg
     5761 cccatcgaga gggcttgatt ttaggttcag cctgttcaga gggtgaagtt tttgacgtgg
     5821 tcgtttctca aggtgtggat gcggcggttg aggtggccaa gtattatgac tttatcgagg
     5881 tcatgccacc ggctatctat gcgcccttga ttgccaaaga gcaggtcaag gatatggagg
     5941 aactccagac cattatcaag agtttgatag aggttggaga tcgccttggc aagcctgttc
     6001 tggctacggg aaatgttcac tatatcgaac cggaagaaga gatttatcgt gaaattatcg
     6061 tccgtagttt gggacagggt gcgatgatta accgaactat cggtcatggt gaacatgccc
     6121 aaccagcacc acttccaaag gctcattttc gaacgactaa tgagatgttg gatgaatttg
     6181 cctttttggg agaggaactg gctcgtaaac tggttattga aaacaccaat gccttggcag
     6241 aaatatttga acccgttgaa gtcgttaagg gtgacttgta tacgcctttc atcgacaagg
     6301 ctgaagaaac agttgctgag ttgacctata agaaagcttt tgagatttat ggaaatccgc
     6361 tgccagatat tgttgatttg cggattgaaa aagaattaac atccatactg gggaatggat
     6421 ttgctgtgat ttatctggca tcgcagatgc tggtgcaacg ttctaatgaa cggggttatt
     6481 tggttggttc tcgtgggtct gtcggatcta gtttcgttgc gaccatgatt gggattacgg
     6541 aagtcaatcc tctctctcct cactatgtct gtggtcagtg tcagtacagt gagtttatca
     6601 cagatggttc gtacggttca ggatttgata tgccccataa ggactgtcca aactgtggtc
     6661 acaaactcag taaaaacgga caggatattc cgtttgagac cttccttggt tttgatgggg
     6721 ataaggttcc tgatattgac ttgaacttct cgggagaaga tcagcctagc gcccacttgg
     6781 atgtgcgtga tatctttggt gaagaatatg ccttccgtgc gggaacggtt ggtacggtag
     6841 ctgccaagac tgcctatgga tttgtcaagg gttacgagcg agattatggt aagttttatc
     6901 gtgatgcaga agtagaacgc ctcgctcaag gagcggcggg tgtcaagcgg acaacaggcc
     6961 aacacccggg gggaatcgtt gttattccga actacatgga tgtctacgat tttacgcctg
     7021 tccagtatcc agcagatgat gtcacggctg aatggcagac cactcacttt aacttccacg
     7081 atatcgatga gaacgtcctc aaactcgatg tactgggaca tgatgatccg accatgattc
     7141 gaaaacttca ggatttgtct ggtattgacc ctaataaaat tcctatggat gacgaaggcg
     7201 tgatggcact cttttctggg actgatgtgc taggggtaac acctgaacaa attggaacgc
     7261 ctacgggtat gttggggatt ccagagtttg gaacaaattt cgtacgtgga atggtagacg
     7321 aaacccatcc gacaaccttt gcggaattgc ttcagctgtc tggtctgtcc cacggtactg
     7381 acgtttggtt ggggaatgct caggatctga ttaagcaagg aatagcggac ctatcgactg
     7441 ttatcggttg tcgggacgac atcatggttt acctcatgca tgcgggtctg gaacctaaga
     7501 tggcctttac cattatggaa cgggtacgta agggtttgtg gctaaagatt tcagaagagg
     7561 agagaaatgg ctatatcgaa gcaatgaagg ctaataaggt gccagagtgg tatatcgaat
     7621 cctgtgggaa aattaagtac atgttcccta aggcccatgc ggcagcctac gttatgatgg
     7681 ccttgcgtgt agcttacttc aaggttcacc atcctattta ttactactgt gcttacttct
     7741 ccattcgtgc taaggctttt gatatcaaga ccatgggtgc gggcttggag gtcatcaagc
     7801 gcagaatgga agaaatctct gaaaaacgga agaacaatga agcctctaat gtggaaatcg
     7861 atctctatac aactcttgag attgtcaatg agatgtggga acgaggtttc aagtttggta
     7921 aattagatct ctactgtagt caggcgacag agttcctcat cgacggggat acccttatcc
     7981 caccatttgt agcaatggat ggtctgggag agaacgttgc caagcaactg gtgcgggcgc
     8041 gtgaagaggg agaatttctc tctaaaacag aactacgcaa gcgtggtgga ctctcatcaa
     8101 ccttggttga aaagatggat gagatgggta ttcttggaaa tatgccagag gataaccagt
     8161 tgagtttgtt tgatgagttg ttttaaaaaa ttgcttaata atctattaaa agaggctaac
     8221 gtatatccaa tagatttaca ttagctttct tttttgttaa aatagtctat ggaaagaggg
     8281 tgagagtatg tcaaagatga gtataagcat ccgtctggat agtgaggtta aggagcaggc
     8341 ccaacaggtg tttagtaatc tgggaatgga tatgacaaca gctattaata ttttccttcg
     8401 tcaggcaatt caatatcagg gactaccttt tgatgttaga ctagacgaaa atcggaagtt
     8461 gctccaagcg ttaacggatt tagaccaaaa tcgtaatatg agccagtctt ttgaatcagt
     8521 ctcagatttg atggaggact tacgtgctta agattcgtta tcataaacag tttaaaaaag
     8581 attttaagtt ggctatgaag cgtggtttga aggcagaatt attagaagaa gttttgaatt
     8641 ttctggttca agaaaaagaa catcctgcca gatatcgtga tcattcattg acggcatcca
     8701 agcattttca aggagttcgt gaatgccata cccagccaga ttggcttttg gtttataaag
     8761 tagacaagtc ggaattgatt ttaaatttgc tgaggacagg cagtcacagt gatttatttt
     8821 aatctatttt aagggggttc tcatgaaact aagaatattt gcggaagata agccggctaa
     8881 gaaggtattt gaatatcaat tagaacttgc tgatcgtaca attcttctat cgacagcact
     8941 cttgtcaggt gctattgctt tagcaggaat cttttctgct ttgagagaaa aataaaaata
     9001 gaaaagagaa aacagaatgg ttttaccaaa ttttaaagaa aatctagaaa aatatgcgaa
     9061 attgttggtt gcgaacggaa ttaacgtgca acctggtcac actttggctc tctctattga
     9121 tgtggagcaa cgtgaattgg cacatctaat cgtgaaagaa gcttatgcct tgggtgcgca
     9181 tgaggtcatc gttcagtgga cagatgatgt gattaaccgt gagaaattcc tccatgcccc
     9241 gatggagcgt ttggacaatg tgccagaata caagattgct gagatgaact atctcttgga
     9301 gaataaggct agccgtcttg gagttcgttc atctgatcca ggtgccttga acggagtgga
     9361 cgctgacaag ctttcagctt ctgctaaagc tatgggactt gccatgaagc ctatgcgtat
     9421 cgcaactcaa tctaacaagg ttagctggac tgtagcagct gcagcaggac ttgagtgggc
     9481 taagaaagtc ttcccaaatg ctgcgagcga cgaagaagca gttgatttcc tttgggacca
     9541 aattttcaaa acttgccgtg tctacgaagc agatcctgtt aaggcctggg aagagcatgc
     9601 agctatcttg aagagcaagg ccgatatgct taataaggag caattttcag cccttcacta
     9661 cacagcgcca ggaacagatt taacacttgg tttgccaaag aaccatgttt gggaatcagc
     9721 tggtgctgtc aatgcacagg gcgaaggatt cttgccaaat atgccgacag aagaggtctt
     9781 cacagcacct gacttccgtc gtgcagatgg ttatgtcact tctacaaaac cgcttagcta
     9841 caacggaaat attattgaag gcattaaggt gacctttaag gatggacaaa tcgtagatat
     9901 cactgctgag aagggtgatc aggttatgaa agaccttgtc tttgaaaatg cgggtgcgcg
     9961 tgccttgggt gaatgtgcct tggtaccaga tccaagtcca atttctcagt caggcattac
    10021 cttctttaac acccttttcg atgaaaatgc gtcaaaccac ttggctatcg gtgcagccta
    10081 tgcgactagc gttgttgatg gagcggagat gagcgaagag gagcttgaag ctgcagggct
    10141 taaccgttca gatgttcacg tagactttat gattggttct aaccaaatgg atatcgatgg
    10201 tattcgtgag gatggaacgc gggtacctct tttccgtaat gggaattggg caaattaagg
    10261 agataatatg ttaggaagta tgttcgttgg tctcctagtg ggatttttag caggtgctat
    10321 gaccaatcgt ggagagcgaa tgggatgttt tggaaaaatg tttctcggtt ggatcggagc
    10381 ctttctaggt cacttgctct ttggaacttg ggggccagtt ttatcaggaa cagctattat
    10441 cccagctgtt ttaggtgcca tgattgtctt agctattttt tggagacgag gaagttaatt
    10501 tcctaaatct gatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc
    10561 tcaaagcact gctttgaggt tgcagataga actgacgaag tcagtaacat atatgcggca
    10621 aggcgacgtt gacgcggttt gaagagattt ttgaagagta tgaaacgaaa aggaggttgg
    10681 tcattgtact agcctccttt tgatatgata taatagttct atgagattag ataaattttt
    10741 agttgcctgt gcggtgggaa gtcggactga ggtcaaaaac ttgctcaagg ctgggcgcgt
    10801 gactgtaaat ggtaaaaaag aaaaatcagc taaattgcag attgatgaaa aaatagatga
    10861 gattcgcttt gatgggcaag tgttggagta tgaagagttt gtctactaca tgatgaacaa
    10921 gcccaaagga gttatctcag cgactgagga tcccaagcac agaaccgttc tggacttgtt
    10981 ggatgacttg gcgcggagta aggaagtttt tccagtagga cgcttggata ttgacacgca
    11041 tggtcttttg ctcttgacca ataatggtca gctggctcat gttcttcttt ctcccaagcg
    11101 tcatgtggac aagacttatc aggctcaggt caagggaatt atgacccaag aagatgtgga
    11161 gacatttatc aagggcattc cgctcaaaga ctttacctgt cagcccgcta gactggagat
    11221 tctttccaca gatgcagaaa agaatcagag ccaaatccgt gtgaccattg cagaagggaa
    11281 gtttcatcag atcaagcgta tggtgggcta ctgtggcaag gaagtggtgg acttgcaacg
    11341 attgactatg ggaactttag tattagatga gaacctggaa cgaggggaat ggcgtcgctt
    11401 gaccaaggaa gaattagaaa ttctccgcgc taatattatt taatttagtt taatactcaa
    11461 tgaaaatcaa agagcaaact aggaaactag ccgcaggctg tacttgagta cggcaaggcg
    11521 acgttgacgt ggtttgaaga gattttcgaa gagtataaat tcgaaaaagg tgagggagaa
    11581 tgtccttacc ttttatgttt tttagttgct tcctttgtga aaagagttat aatagactgt
    11641 agaataaaag gaggaatcta tgaacgcgat tcaagaatca tttactgata aactatttgc
    11701 caactatgaa gcaaatgtaa aataccaagc gattgaaaat gctgccagcc acaacggaat
    11761 ttttgcagct ctggaacgtc gccaaagcca tgtagacaac acacctgttt tctcattgga
    11821 tttaaccaag gacaaggtca ctaaccagaa agcgtctggt cgttgctgga tgtttgcggc
    11881 tctcaacacc ttccgccaca aactcatctc gcaatacaaa ttggagaact ttgagttgtc
    11941 acaagcccac actttcttct gggataagta tgagaaatca aactggttct tggagcaagt
    12001 cattgcgact tcagaccaag aattgactag ccgcaaggtt agcttcttac tccaaacacc
    12061 tcaacaagat ggtggtcaat gggatatggt cgtttccctc tttgaaaaat acggtgtcgt
    12121 gcctaagtca gtttatcctg agtctgtttc atctagcagc agtcgtgagc taaatgcgat
    12181 ccttaataaa ttgcttcgtc aagatgctca aatcttgcgt gatttgcttg tttctggtgc
    12241 agatcaagtg actgttcaaa ctaagaaaga agacctcttg caagaaatct ttaactttct
    12301 tgctatgtca ttaggacttc caccacgcaa gtttgacttt gcttatcgcg ataaagataa
    12361 caactacaaa agtgaaaaag gaatcacacc acaagagttc tacaagaaat atgtcaatct
    12421 tcctttagaa gactacgttt ctgttatcaa tgctccaact gctgataaac cttacggaaa
    12481 atcttacaca gttgagatgt tggggaatgt ggttggtagc cgtgcagttc gctacatcaa
    12541 cgttccaatg gagcgcttga aagaattggc gattgcccaa atgcaagcag gtgagactgt
    12601 ttggtttggt tctgatgtcg gccagctcag caaccgtaag gctggcatcc ttgcgacaga
    12661 tgtttatgac tttgaatcaa gcatggacat taaacttact caagacaagg ctggacgttt
    12721 ggactatagt gaaagcttga tgacccacgc catggtcttg acaggtgttg acttggacga
    12781 aaatggtaaa tcaaccaagt ggaaggttga aaactcatgg ggagacaagg tcggtacaga
    12841 tggttacttt gttgcctcag acgcttggat ggacgaatac acataccaaa tcgttgttcg
    12901 taaggaattg ctgacagcag aagaacaagc tgcctatgga gcagaaccaa tcgtacttgc
    12961 accatgggat ccaatgggag ccttggctga ataaaagcat agaaaaaagg aatcagattt
    13021 tagaacctgg ttccttttta gttgcttgat tacatgatgt gaagaacatg tgccacaata
    13081 cccactgcga agagggcaag gataatagtg attggagata cttttttctt aagtaagtac
    13141 atgcaaagta aagtaaggag tagtcctgat aatccaggaa tcaacatatc caagttttgt
    13201 tggaaagtag taactttttc aggagtttga gacaatcctt gtcctacttg tgcgaatgct
    13261 tcttggatac ctttagaccc ttctggcaat ttatcccaat ggatataagc cttttcatct
    13321 agttgaactt tagaaacatc gaaagcaaat ttaatattta cccagcgttg aacaaggaca
    13381 gcaagaatga acatcccaag gatagaagct cctttagtga tatcttgaag gataccacca
    13441 gacatatctt tagtgatttc tgatccagcc ttgtatccaa tctcttgaac ataccacaag
    13501 aatgacatac gaatcaagtt ccatgcaaca aagaagagga gtggacccaa gatattgcca
    13561 gtaagggcaa gtgaagcacc gagagatcca aggattgggc gtactgtaaa ccagaatact
    13621 gggtcaccga taccagcaag aggtcccatc ataccgattt taaccccttg gatagcagcg
    13681 tcatcgattt ccacaccgtt agcacgttct tcttcaagcg caagagtaac ccccatgact
    13741 ggagcagcta cgtatggatg agtgttgaag aactcaaggt gacgctcaag agcagcgatt
    13801 tgatcttctt ttttagtata gagttttttg atagctggaa tgagtgtata agcccagccc
    13861 aagttttgca tccgttcaaa gttccaagac ccttgtaaga aggttgaacg ccaccaaact
    13921 tttttacgat ctgatttagt taattgaagt ttttcagtca tgatgttttc agtcctttct
    13981 tatcttagta gtcttctagg atatcgccga ttgggtcgtt agaagttgcg gctcctccgc
    14041 caccatttcc accagtttta gaaaggtgaa ggtagataag agcgatagca acaccgatag
    14101 caccgaatcc gattagagta atatctgaca cagcagcgag aacgaaacca agagcgaaga
    14161 atggccatac ttcacgagtt gccatcatgt tgataaccat ggcgtaacca acggcaacga
    14221 ccataccacc accgatagcc ataccatctt tgagccagtc tggcatggca ctaaggatac
    14281 tttgtacagt ttcagttggt accataagga gaagagctgc aggaagcgcg atacgaagtc
    14341 cttggaaaag tagcgcaatg aaatgcgcac gctccacagc gccgaagtca ccttttttag
    14401 cggcagcatc tgcagtatga accaaaccaa ctgaaattgt acgaacaatc attgtcaaga
    14461 aaagtccagc tacggcaaga gggatagcaa ccgcttgggc aacaccgata ccagtcttgg
    14521 taaagtcacc accaagaacc atgataatgg cagcagcgac agaagcaagt gcagcatcag
    14581 gagcgatagc agcaccgata tttgaccaac caagggcaat catttgaagc gatccaccga
    14641 ggataatccc tgcttccaag tgacctgtta caagcccaat aagggtacag gctacaagtg
    14701 gttggtgaaa ttggaactgg tcgaggatgc cttcaagacc tgcaaagaag gctacaacga
    14761 ctactaaaac catagaaata atagacatgt ttaaaatcct ttcataaata atggcttatt
    14821 tgacattggc tttgttaatc aagtcaaaca aatctttttt agaatcattt ggtactttac
    14881 ggacatcaaa ttcaacaccc aagtcacgca ttttttcaaa tgtagcaaca tcttctttgt
    14941 ccatagacaa aacggtattg accaatgttt tacctgttga gtgagccata gaaccaacgt
    15001 taagagtctt gattggcacg ccgccttcga tggcacgaag ggcatcttga ggtgtttcaa
    15061 acaagataag ggcatgtgtt tctccaaaac gtgggtcttt tgaaatctca atcagttttt
    15121 gaattggaac cacgttagcc ttgacattac ctggagctgc ttgtttaatc aattctttac
    15181 gaaggtcgtc tttagccacg ttatctgaag caacgatgat acgatttgct tttgaatctg
    15241 gagtccaagc agttgcaacc tgaccgtgaa gtagacgtgt gtcaagacgg gcaagattga
    15301 ttttcaattt accgtctccg ataacagttc cttctgggat agcagtttgg gcaactggag
    15361 cagctgcagc gcttgcaact tcttcaactg gatttagctc ttctggaaga gctttgatgc
    15421 catctttggc ttctttaatg atattagcag cgactttttc tacacctgca gcagcgtcca
    15481 tgaggcgctc tgtgtaggct tgaatcaaca tcggtaagtt aagtcctgtg atgatggcaa
    15541 acttacgctc aggattttct cccatcacgc gactagcttg gttaaatgga gaaccactcc
    15601 aaaggtcagc caaaactaga acctcatctt ctgcgtcaaa tgcagcaaca gcgttattaa
    15661 acttagcgta tagatcatca ggaccttcat ttggcataaa ggtcacaact tgaacctttt
    15721 cttgttcacc aaagatcata gatcctgact gatgaatacc cgcagcaaat tcgccgtggc
    15781 tcgcaataat gattccgata ctcattattg tcattcctcc ttttttgttt tagttttcat
    15841 ttaagaa
//
LOCUS       DAVLYR010000028        15382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLYR010000028 DAVLYR010000000
VERSION     DAVLYR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.028"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            85..2307
                     /gene="recJ"
                     /locus_tag="VYO63_000746"
     CDS             85..2307
                     /gene="recJ"
                     /locus_tag="VYO63_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1671706.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2482..4143
                     /locus_tag="VYO63_000747"
     CDS             2482..4143
                     /locus_tag="VYO63_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1671707.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4212..4991
                     /locus_tag="VYO63_000748"
     CDS             4212..4991
                     /locus_tag="VYO63_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1671708.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5103..6323
                     /gene="murM"
                     /locus_tag="VYO63_000749"
     CDS             5103..6323
                     /gene="murM"
                     /locus_tag="VYO63_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1671709.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6328..7560
                     /gene="murN"
                     /locus_tag="VYO63_000750"
     CDS             6328..7560
                     /gene="murN"
                     /locus_tag="VYO63_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1671710.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7639..8340
                     /locus_tag="VYO63_000751"
     CDS             7639..8340
                     /locus_tag="VYO63_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1671711.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            8379..10223
                     /gene="uvrC"
                     /locus_tag="VYO63_000752"
     CDS             8379..10223
                     /gene="uvrC"
                     /locus_tag="VYO63_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1671712.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNEHYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKNDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVTAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            10213..11046
                     /locus_tag="VYO63_000753"
     CDS             10213..11046
                     /locus_tag="VYO63_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1671713.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLQGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSKGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQRHNLKLLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(11224..12024)
                     /locus_tag="VYO63_000754"
     CDS             complement(11224..12024)
                     /locus_tag="VYO63_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671714.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTYLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12195..12800
                     /locus_tag="VYO63_000755"
     CDS             12195..12800
                     /locus_tag="VYO63_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1671715.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVCEKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12813..14213
                     /gene="pepV"
                     /locus_tag="VYO63_000756"
     CDS             12813..14213
                     /gene="pepV"
                     /locus_tag="VYO63_000756"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1671716.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14267..14845
                     /locus_tag="VYO63_000757"
     CDS             14267..14845
                     /locus_tag="VYO63_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1671717.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15005..15157
                     /locus_tag="VYO63_000758"
     CDS             15005..15157
                     /locus_tag="VYO63_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1671718.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LTDSYD"
     gene            15200..15292
                     /locus_tag="VYO63_000759"
     CDS             15200..15292
                     /locus_tag="VYO63_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1671719.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
BASE COUNT         4634 a         2791 c         3598 g         4359 t
ORIGIN      
        1 tataaaaaag caatctggat agagaaaacg tctaaatcat gttataatga agcaatagaa
       61 ttcttagaaa gagtggatgt ctttttgata acacctactt atgaatggca gtttgccctg
      121 caggtagaag atgcggattt tacaaagata gccaagaagg ctggactggg tcctgaggtg
      181 gctcggttat tgtttgagag agggattcag gaccaagaaa gtctgaagaa gtttttagaa
      241 ccttccttgg aggacttaca tgatgcttat ctgctccatg atatggacaa ggcagtggag
      301 cggattcgtc aggctattga agaaggggaa aatattctcg tttatggaga ctatgatgcg
      361 gatggcatga cttcggcttc tattgtgaag gaaagtttgg aacaacttgg tgctgagtgc
      421 cgagtttacc tgccaaatcg ttttaccgat ggctatggcc ctaatgctag tgtttataaa
      481 tactttatcg agcaagaagg aatttccttg attgtgacgg tggacaatgg ggttgctggt
      541 catgaggcta ttgcattggc tcagtctatg ggagtagatg tcattgtgac agaccatcat
      601 tccatgcctg aaaccctgcc agatgcttat gctattgtcc atcctgaaca tccagatgcg
      661 gattatcctt ttaaatattt ggctggttgt ggagttgctt tcaagttggc ttgtgccctg
      721 ttagaagaag tgcaagtgga attgcttgat ttggtcgcta ttggaactat tgcagatatg
      781 gtgagtctga cggatgaaaa tcgtatctta gttcaatatg gtctggaaat gttgggtcat
      841 acccagcgca ttggtctgca agaaatgctg gacatggctg ggattgctgc caacgaagta
      901 acagaagaaa cggttggttt ccagattgct cctcgtttga atgccttggg tcgcttggat
      961 gatcccaatc ctgccattga tttgttgact ggatttgatg atgaggaagc gcatgagatt
     1021 gcccttatga ttcaccagaa aaacgaagag cgcaaggaaa tcgttcagtc tatctatgaa
     1081 gaagccaaga ccatggtgga tcctgagaag aaggttcagg tcttggccaa ggaaggctgg
     1141 aatcctgggg ttctaggaat cgtggctggt cgtttattgg aagaattggg acagacagtc
     1201 attgttctta atatagaaga cggtcgtgcc aagggcagtg ctcgtagtgt ggaagcggtc
     1261 gatatttttg aagctctgga tccccatcga gacctcttca tcgcctttgg aggtcatgca
     1321 ggtgcagcgg gtatgacgct ggaagttgag caactctcag atttatctca ggttttggaa
     1381 gattatgttc gtgaaaaagg tgcagatgct ggtggcaaga ataagttaaa cctagatgaa
     1441 gagttggatt tggaggcact tagcttggaa acggtcaaaa gttttgaacg tttagctcct
     1501 tttggaatgg ataatcagaa acctattttt tatatcaaga attttcaggt cgaaagtgct
     1561 cgtactatgg gggcaggtaa tgcccatcta aagctgaaaa tttccaaggg tgaggcgagt
     1621 tttgaagtgg tagcctttgg tcaaggcaga tgggcgacag agttttctca aaccaagaat
     1681 ctagagttag cggttaaatt gtctgtcaac caatggaatg gccaaactgc cctccagttg
     1741 atgatggtgg atgcgcgagt ggaaggtgtt caacttttta acattcgtgg aaaaaatgca
     1801 gtcttgccag aaggtgttcc agtcttggat tttcctggag aactgccaaa tcttgcggct
     1861 agtgaagctg ttgtcgtaaa aaacattcca gaggatatta ctcagctgaa gaccattttt
     1921 caggaacagc atttctctgc tgtctatttc aaaaatgata ttgacaagac ctattatctg
     1981 acaggttatg ggactagaga tcagtttgct aaattgtaca agactattta ccagttccca
     2041 gagtttgata ttcgctacaa gctgaaagat ttggctgcat atcttaatat tcaacaaatc
     2101 ttgctggtca agatgattca agtatttgaa gaactaggct ttgtgacgat aaaagatggt
     2161 gtgatgacag tcaataaaga ggcgccaaag cgggagatag gagaaagtca aatttaccaa
     2221 aatctcaaac aaaccgttaa agaccaagaa atgatggcgc tgggtacggt gcaagaaatt
     2281 tatgattttt tgatggaaaa agagtagaag ttaggaaaga gttgggaaat caactctttt
     2341 ttgaaaacag accttcattt tgaaaatcat caaaaaaatg gtataatggt aggaaaagat
     2401 tcggctgaaa gtatcagaac ttttagaata agagggtaga attgccctat aatcaagata
     2461 aactaagatt ttggaggaaa aatgagtaat atcagtttaa caacacttgg tggtgtgcgt
     2521 gagaatggaa aaaatatgta cattgctgaa attggagagt ccatttttgt tttgaatgta
     2581 gggttaaaat atcctgaaaa tgaacaatta ggggtcgatg tggtgattcc aaacatggat
     2641 tacctttttg aaaatagcga ccgtattgct ggggttttct tgacccacgg gcatgcggat
     2701 gccattggtg ctctacctta tctcttggca gaggctaaag ttcctgtatt tgggtctgag
     2761 ttgaccattg agttggcaaa gctctttgtc aaaggaaatg atgccgttaa gaaatttaat
     2821 gatttccatg tcattgatga gaatacggag attgattttg gtgggacagt ggtttccttc
     2881 ttccctacga cttactccgt tccagagagt ctgggaattg tcttgaagac atcggaagga
     2941 agcatcgttt atacaggtga cttcaaattt gaccaaacgg ctagtgaatc ttatgcaact
     3001 gattttgctc gtttggcaga gattggtcgt gacggcgtcc tggctctcct cagtgattcg
     3061 gccaatgcag acagcaatat tcaggtggct agtgaaagtg aagttaggga tgaaattacc
     3121 caaactattg ctgactggga aggtcgtatc atcgttgcag ctgtttccag taatctttct
     3181 cgtattcagc agatttttga cgctgcggat aaaacaggtc gacgtatcgt cttgacagga
     3241 tttgatattg aaaatatcgt ccgcacagcg attcgtctta agaagttgtc tttagccaac
     3301 gaaattctct tgattaagcc taaagatatg tctcgctttg aagaccatga gttgattatt
     3361 cttgagacag gtcgtatggg tgaacctatc aatggacttc gtaagatgtc gattggtcgc
     3421 catcgttatg tagaaatcaa ggatggggac ctggtctata ttgctacggc tccgtctatt
     3481 gctaaagaag cctttgttgc gcgtgtggaa aatatgattt atcaggcagg tggggttgtc
     3541 aaattgatta cccaaagttt acatgtatca gggcacggaa atgtgcgtga tttgcagctg
     3601 atgatcaatc ttttgcaacc taagtacctc ttccctgtcc aaggggagta tcgtgagttg
     3661 gatgctcacg ctaaggctgc catggcagtt gggatgttgc cagaacgcat tttcattcct
     3721 aaaaagggga cgaccatggc ttacgagaat ggagactttg ttccagctgg atcggtttca
     3781 gcaggagata tcttgattga tgggaatgcc attggtgatg ttggaaatgt tgttcttcgt
     3841 gaccgtaagg tcttgtcaga ggatggaatt ttcatcgtgg ctattacagt caaccgtcgt
     3901 gagaagaaaa ttgtggctag ggctcgtgtt cacacgcgtg gatttgttta tctcaagaag
     3961 agtcgcgata ttctccgtga aagttcagaa ttgattaacc aaacggtaga agattatctt
     4021 caaggagatg actttgactg ggcagatctt aaagggaagg ttcgagataa tttgaccaag
     4081 tatctctttg accaaaccaa gcgtcgtcca gctattttac cagtagtcat ggaagcaaaa
     4141 taatcgttga aataaacaga gagaaagtcg agtttcggct ttttcttata gaaaaataga
     4201 aggagaaaat catggcagta atgaaaatcg agtattactc acaagtattg gatatggagt
     4261 ggggggtgaa tgtcctctac cctgatgcca atcgagtgga agaaccagag tgtgaagata
     4321 ttcccgtctt gtaccttttg cacgggatgt ctggaaatca taatagttgg cttaagcgga
     4381 ccaatgtaga acgcttgctt cgaggaacta atctcatcgt tgttatgccc aataccagca
     4441 atggttggta caccgatacc cagtatggtt ttgactacta cacggctcta gcagaggaat
     4501 tgccacaggt tctgaaacgc ttcttcccta atatgactag caagcgtgaa aagaccttta
     4561 tcgctggtct ttctatggga ggctacggct gcttcaaact ggctcttacg acaaatcgtt
     4621 tttctcatgc agctagtttt tcaggtgccc tcagctttca aaacttttct cctgaaagtc
     4681 aaaatctggg aagtccagcc tactggagag gtgtttttgg agagattaga gactggacaa
     4741 ctagtcccta ttctcttgaa agtctggcta aaaaatcgga taaaaagacc aaactttggg
     4801 cgtggtgtgg cgaacaggat ttcttgtacg aagccaataa tctcgcagtg aaaaatctca
     4861 aaaaactagg ttttgatgtg acctatagcc atagtgctgg aactcacgag tggtactact
     4921 gggaaaaaca attggaagtt tttttaacaa ccctaccaat tgatttcaaa ttagaagaga
     4981 gactgactta gtttgaactt cagcataggg ggagtagaac taaaataaaa tatgttttca
     5041 ctagactttt caaacgaaag tagtagaata gtaataaaat actggaggaa agagagtagg
     5101 aaatgtaccg ttatcaaatt ggcattccca cattagaata tgatcagttt gtcaaagaac
     5161 atgaattagc caatgtatta caaagtagtg cttgggagga agttaagtct aattggcaac
     5221 atgagaagtt tggtgtttac agggaagaaa aattactggc gacagctagt attttgatta
     5281 gaactcttcc gctaggctat aaaatgtttt acatcccaag aggacctata ttggattatg
     5341 gggataaaga actcttgaat tttgccattc agtctattaa gtcctatgct cgcagtaaga
     5401 gagcggtttt tgtgactttt gacccaagta tttgcctatc tcaaagttta atcaatcagg
     5461 aaaagacaga atttcctgaa aatctggcta ttattgatag tttgcaacaa atgggagtaa
     5521 ggtggtcagg aaaaacggag gaaatgggag acaccattca acctcgtatt caggcgaaaa
     5581 tatacaagga aaattttgaa gaagataaac tttccaagtc aacaaaacag gctattcgaa
     5641 cagcacgaaa caaagggctt gagattcaat atggtggact ggaactatta gattcatttt
     5701 cggagttgat gaaaaaaact gagaagcgaa aagagattca tttgaggaat gaagcctatt
     5761 ataaaaaatt gttagataat tttaaggaca aggcctatat caccttggcc accttggatg
     5821 tttctaaacg ttcgcaagaa ttagaagaac agttagcgaa aaatagagcc ttggaagaga
     5881 cctttactga gtcgactcga acttcaaaag tagaagcgca gaagaaggaa aaagaacgtt
     5941 tgttagagga attgaccttc ttgcaggaat atatagatgt aggtcaagcg agagttcctt
     6001 tagcggctac tttgagtttg gaatttggta ctacctctgt caatatatat gctggtatgg
     6061 atgatgattt taaacgttac aatgcaccaa ttttaacatg gtatgaaacg gctcgctatg
     6121 cctttgagcg aggtatggtc tggcaaaatt taggtggtgt tgaaaactct ctcaatggtg
     6181 gactttatca ttttaaggaa aaatttaatc caacgattga agaatacttg ggtgaattta
     6241 caatgcccac tcatcctctc tatcctctgt taagacttgc tcttgatttc cgtaaaacat
     6301 taagaaaaaa acatagaaag taagtatatg gcactaacaa cactcacgaa agaagagttt
     6361 cagacttatt ctgatcaggt ttcttctcgt tcctttatgc aatctgtcca gatgggggat
     6421 ttgctagaaa aaagaggggc tcgaattgtt tatcttgctt tgaaacaaga aggagaaatt
     6481 caagttgcag ctctggttta tagcttgccc atgctgggtg gtctgcatat ggaactcaat
     6541 tcggggccga tttataccca acaagatgct cttccagttt tttatgcaga gttaaaagaa
     6601 tatgccaagc aaaatggtgt attagagttg cttgtaaaac cttatgaaac ttatcaaact
     6661 tttgatagcc aaggtaatcc aatagatgct gagaaaaaaa gtattattca aggtttgact
     6721 gatttaggtt atcaatttga tggcttaaca acaggttacc caggtggaga accagattgg
     6781 ttatactata aagatttaac tgaattaact gaaaagagtt tgcttaaaag ttttagcaaa
     6841 aagggtaaac ccttggtgaa aaaggctgaa acctttggca ttcgattgaa aaagttaaaa
     6901 cgtgaagaac tatcgatttt taagaatata acaaaagaaa cctctgaacg tagagaatat
     6961 agtgataaaa gtttagaata ttatgagcat ttttatgata cttttggaga acaagcggag
     7021 tttctcatag caagcttaaa tttttcggac tatatgagca aattgcaagg tgaacaaaat
     7081 aaactagaag aaaacttgga caagttgcga cttgatttga gtaaaaatcc tcattctgag
     7141 aaaaaacaaa atcaactgag agaatattct agtcaatttg aaacgtttga agttcgaaaa
     7201 gcagaagcgc gagacttgat tgaaaaatat ggagaagaag atattgtttt agctgggagt
     7261 ttatttgttt atatgcctca ggaaacgact tatctcttta gtggttccta cactgagttt
     7321 aataagttct atgcccctgc actgcttcaa aaatatgtta tgttggaaag cataaaacgt
     7381 ggaataccta aatacaactt cctaggcatt caagggattt ttgatggaag tgatggtgtt
     7441 ttgcgtttta aacagaattt taatggctat attgtacgca aagcaggtac tttccgttac
     7501 catccatctc ctttaaaata caaagctatc cagttactca aaaaaatagt aggacgttaa
     7561 gatgaaaaag tcagtattta gatttctttt agcttctttt agtaaaataa tttttatttg
     7621 ctagaaaggt ggagagacat gcgctggctt tttcgtttga taggggcttt cttttctttt
     7681 gtgtggcgtt tgttttggcg tctggtttgg atagttgtgc tcttatgtgt gcttgctttc
     7741 ggacttctct ggtatctgaa cggggatttt caaggagcgc taaagcaagc agaacggtca
     7801 gtaaaaattg gtcaacaaag tattgaccaa tgggagaaaa cagggcaact gcctaagtta
     7861 agccagacag atagtcacca gcattctgaa ggaaggtggc cacaggcctc tgctcgtatt
     7921 tacctggatc cgcagatgga ttcacgcttt caagaggctt atttagaagc aatccagaac
     7981 tggaatcaaa ctggtgcttt taactttgaa ctcgtgactg agtctagtaa ggcggatatt
     8041 acggctacgg agatgaacga cggaggcact cctgtggcag gagaggcgga aagtcaaact
     8101 aatctcttaa cagggcaatt cttgtccgta acggtgcggt tgaatcatta ttatttgtcc
     8161 aatccatact atggctattc ctatgaacgc cttgtccata cggcagaaca tgagttaggt
     8221 catgcgattg gcttggacca tacagatgag aagtctgtca tgcaaccagc aggttccttt
     8281 tatggtatcc aggaagagga tgttgcaaac ctccgaaaaa tatatgagac taatgagtag
     8341 ggtactatct ttccctactt ttttgctata atggaactat gaacaacttg attaaaccaa
     8401 aactagagct cttgccgacc agccctggtt gctacattta caaggataaa aacggcacca
     8461 ttatctatgt aggaaaggct aaaaatctgc gtaatcgcgt gaggtcttat ttccgtggaa
     8521 gtcatgatac caaaacagag gccttggtgt ctgaaattgt ggattttgaa tttattgtta
     8581 cggagtctaa tattgaggcg cttctgctgg aaatcaacct gatcaaggaa aataagccca
     8641 agtacaatat catgctcaag gatgacaagt cttatccttt tatcaaaatc accaatgagc
     8701 actatccacg gcttatcatt actcgtcagg tcaaaaagga cggtggtctt tattttggcc
     8761 cttatccaga tgtgggggca gccaatgaaa tcaagcgact tctagatagg attttccctt
     8821 ttcgaaaatg taccaacccg ccctctaagg tctgttttta ctaccatatc ggccaatgta
     8881 tggcccacac catctgtaag aatgacgagg cctatttcaa gtccatggcc caggaggtgt
     8941 ctgattttct gaaaggtcag gatgacaaaa tcatcgatga tctcaagagt aaaatggcag
     9001 taacagcaca aagtatggag tttgaacggg cagcggaata ccgtgacctg attcaggcca
     9061 ttggaacact tcgaaccaag caacgggtca tggctaagga tctccaaaat cgcgatgtct
     9121 ttggctacta tgtggataag ggctggatgt gtgtgcaggt tttctttgtt cgtcagggca
     9181 agctcatcga gcgcgatgtc aatctcttcc cctacttcaa tgatccagat gaggatttct
     9241 tgacctatgt gggacaattc tatcaagaaa aatctcacct agttcccaat gaggtactga
     9301 ttccgcagga tattgacgaa gaagctgtca aggctttggt ggataccaag attctcaaac
     9361 cccaacgtgg agagaaaaaa cagctggtca atctagccat aaaaaatgct cgtgttagtc
     9421 tagagcagaa gttcaatctg ctagaaaaat cagtcgaaaa gacccaagga gctattgaaa
     9481 atctaggacg tttgctccaa attccgactc cagttcgcat cgagtccttc gataactcca
     9541 acatcatggg gaccagtcct gtttcagcca tggtggtctt tgttaacggc aggccaagta
     9601 aaaaagatta ccgtaagtac aagataaaaa ccgttgttgg gccggacgac tatgctagta
     9661 tgagagaagt cattcgcaga cgctatggtc gagtacagcg tgaggctttg actcctccag
     9721 atttgattgt gattgatggg gggcaaggtc aagtcaatat tgctaagcaa gtcatccaag
     9781 aggaactggg cttggatatt ccaattgctg ggctgcaaaa gaatgataag caccaaaccc
     9841 atgaattgct ctttggagat ccgcttgagg tggtggactt gtctcgcaat tctcaggaat
     9901 ttttcctcct ccaacgcatc caagatgagg tgcaccgctt tgctatcact ttccaccgcc
     9961 aactgcgctc caaaaattct ttctcatctc aattggatgg gattgacggt ctgggaccta
    10021 aacgcaagca gaatcttatg aagcatttca agtctttgac caaaatcaag gaagccagtg
    10081 tggatgagat tgtcgaagtt ggggtaccta gagtcgttgc agaggctgtg caaagaaagt
    10141 tgaacccgca gggagaagcc ttgcctcaag tagcagaaga aagagtagat taccaaacgg
    10201 aaggaaacca caatgaacca taaaatcgca attttatcag atgttcatgg caatgcgacg
    10261 gcgctagaag cagtgattgc agatgctaaa aatcaagggg ccagtgaata ttggcttcag
    10321 ggagatattt ttcttcctgg tccaggcgca aatgacttag tcgccctgct aaaggacctt
    10381 cctatcacag caagtgttcg aggcaattgg gatgatcgtg tccttgaggt cttggatggc
    10441 gaatatggtt tggaatatcc gaaagaaatc cagtcaatgc gcatgaccca gtttttgatg
    10501 gagcgaatgg atcctgcaac gattgtctgg ctacgaagct tgcctttgct ggaaaagaaa
    10561 gaaattgacg gattgcgctt ttctatctct cataatttac ctgacaaaaa ctatggtggt
    10621 gacttgctag ttgagaatga tacagagaaa tttgaccaac tgctagatgc ggaaacggac
    10681 gtggcagttt atggtcatgt tcacaagcag ttgcttcgct acggcagtaa agggcaacaa
    10741 atcatcaatc cagggtcgat tggcatgccc tattttaatt gggaggcgtt aaaaaatcac
    10801 cgtgcccagt atgccgtgat agaggttgaa gatggggaat tactcaatat ccaatttcgt
    10861 aaagttgctt atgattacga ggctgagtta gaattggcca aatccaaggg gcttcccttt
    10921 atcgaaatgt atgaagaact acgtcgtgaa gataactatc agaggcacaa tctgaaacta
    10981 ttagcaagct taatagaaaa gcataggtat gtagaggatg tgaagaattt ttttgatttt
    11041 ttgtaagagt ttcctaaaat aaccaatgca aactaaaaac gattggtagt ccaatcgctt
    11101 ttatatatct tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgtaggttgc
    11161 tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg tttgaagaga ttttcgaaga
    11221 gtgttattgt aactgagatt gatctgggag gtaagaacca cctagatagg tattgctgag
    11281 tttttcaagg gttccgtctt gatagagttc tttgagcgct ttatcaaatt tctctttaaa
    11341 ctctttttgg tcacttgaga aaacgatata gttgctgggg ctatctgtag aaggtaaatc
    11401 aacgactgag aggtctaaac cacggtcctt gataatcttt tgaacggata ccttgtcaaa
    11461 aactaggaaa tcaaactctc cgttagcaag gtctaggatt cgtttaccaa tatcctcacc
    11521 agaaaaatca attgtagcgg gattatcagt gtgtttctga ttccagttat tgatgaattg
    11581 agcgttagaa gttccggtat cctcttgtgt tgttttacca gcgatctggt caagataagt
    11641 caaaggattt ttcttgttgc tgacaaggac gaggggattg ttggaaattg gaagcgagta
    11701 gaggtatttt tcagcgcgct cttttgtgta actcaaatta ttagctgctg cttgatagtg
    11761 accagaatca agtcctggga agatgctctc ccaagcagtt ctttggaatt gaatctcgta
    11821 atcgctgagt ttttcatcca ctgcttttag gacttcgata tcgaagcctg tcagattgcc
    11881 cttgtcttcg tagtcaaatg gtggcacatc gccagctgta gcaaggacga ttgtcttttg
    11941 agagctggac tctttgggtg tagcttgatt cccacaggca accaaaaatg gtaggatagc
    12001 tagtaatagg ctaaattttt tcatactgtc tccattcaaa tgtaaagtac ttgctagttt
    12061 atcagaaact ttcctgataa accaatatat atcttttatg tctgtgatag aaaaacttaa
    12121 tcatgaaaaa tccctgcaag ctctttcaaa ttatggtaga atggaagcag tagaattaga
    12181 aaaggaaatc gattatgaaa tttcttgaat taaataaaaa acgtcatgcg accaagcatt
    12241 tcactgataa gcctgttgat ccaaaagatg tgcgtacggc tatcgaaatt gcaaccttgg
    12301 cgccaagcgc ccacaacagc cagccttgga aatttgtggt ggtatgtgag aaaaatgctg
    12361 aactggcaaa gttagcttat ggttccaatt ttgaacaggt atcatcagca cctgtaacca
    12421 ttgccctatt tacagataca gaccttgcca aacgcgctcg taagattgcc cgagttggtg
    12481 gtgctaacaa tttctcagaa gaacaacttc aatacttcat gaaaaatttg ccagctgaat
    12541 ttgcacgcta caatgaacaa caagtcagcg actacctagc actcaatgca ggtttggttg
    12601 ctatgaactt ggttctcgct cttacagacc aaggaattgg ttccaatatt atccttggtt
    12661 ttgacaaatc aaaagttaat gaagttttgg aaatcgaaga ccgtttccgc ccagaactct
    12721 tgatcacagt gggttataca gacgaaaaat tggaaccaag ctaccgcttg ccagtagatg
    12781 aaatcatcga gaaaagatag aaagaagaaa aaatgacagc aattgatttt acagcagaag
    12841 tagaaaaacg caaagaagac ctcttggctg acttgtttag ccttttggaa atcaattcag
    12901 aacgtgatga cagcaaggct gatgcccagc atccatttgg gcctggtcca gtaaaagcct
    12961 tggagaaatt ccttgaaatc gcagaccgcg atggctaccc aactaagaat gttgataact
    13021 atgcaggaca ttttgagttt ggtgatggag aagaagttct cggaatcttt gcccatatgg
    13081 atgtggtgcc tgctggtagc ggttgggaca cagaccctta cacaccaact atcaaagatg
    13141 gtcgccttta tgcgcgcggg gcttcggacg ataagggtcc tacaacagct tgttactatg
    13201 gtttgaaaat catcaaagaa ttgggtcttc caacttctaa gaaagttcgc ttcatcgttg
    13261 gaacagacga agaatcaggc tgggcagaca tggactacta ctttgagcac gtaggacttg
    13321 ccaaaccaga tttcggtttc tcaccagatg ctgaatttcc aatcatcaat ggtgaaaaag
    13381 gaaatatcac ggaatacctc cactttgcag gagaaaatac aggtgttgcc cgtcttcaca
    13441 gctttacagg tggtttacgt gaaaatatgg taccagaatc agcaacagca gtcgtttcag
    13501 gtgacttggc tgacttgcaa gctaaactag atgcctttgt tgcagaacac aaacttagag
    13561 gagaactcca agaagaagct ggcaaataca aggtgacgat cattggtaaa tcagcccacg
    13621 gtgctatgcc tgcttcaggt gtcaatggcg caacttacct tgccctcttc ctcagccagt
    13681 ttggctttgc tggtccagcc aaagactacc ttgacatcgc aggtaaaatt ctcttgaacg
    13741 atcatgaggg tgaaaatctt aagattgctc atgtggatga aaagatgggt gctctttcta
    13801 tgaatgccgg cgtcttccgc ttcgatgaaa caagtgctga taataccatt gccctcaaca
    13861 tccgctatcc aaaaggaaca agtccagaac aaatcaagtc aatccttgaa aacttgccag
    13921 ttgtttctgt tagcctgtct gaacacggtc acacgcctca ctatgtgcca atggaagatc
    13981 cacttgtgca aaccttgttg aatatctatg aaaaacaaac tggccttaaa ggtcatgaac
    14041 aagtcatcgg tggtggaacc tttggtcgct tgctagaacg cggagttgcc tacggtgcta
    14101 tgttcccaga ctcgattgat accatgcacc aagccaatga atttatcgcc ttggatgatc
    14161 ttttccgagc agcagcaatt tatgccgaag ctatttacga attgatcaaa taaaacgata
    14221 gaagtctgag atcttatgct tggacttctt tttggaggga aagtagatgt ctcaaatcga
    14281 aagaatcaaa caggctatca tggcggattc acagaatgcc agctatacag agcgtggcat
    14341 tgagcctctc tttgcagcgc caaaaactgc tcgcatcaat atcatcggtc aggctccggg
    14401 acttaaaact caagaagcag gcctttactg gaaagataaa agtggtgacc gcttgcggga
    14461 ctggctaggt gtggatgaag atacctttta caattcaggt tattttgctg ttttgcctat
    14521 ggatttctac tttccaggac atggcaagtc gggtgatctt ccgcctcgta caggttttgc
    14581 agaaaaatgg catccgcagc tcttacagga attgcctgat attcagttaa ccctcttgat
    14641 tgggcaatat gcccaagcct actatttaca ggagaaaatc agtgggaagg taacggagag
    14701 ggtgaaacac tataaagact atctgccagc ctattttccg ctagttcacc catcaccacg
    14761 aaatcaaatc tggatggcca aaaatccttg gtttgaggca gaagtagtgc cagatttgaa
    14821 aaaaagaatt aaaaccattt tatagtcaat gaaaatcaaa gagcaaacta ggaagctagt
    14881 cgtaggctgc tcaaagtaca gctttgaggt tgcagataaa actgacgaag tcggtaacat
    14941 acgcacggta aggcgacgct gacgtggttt gaagagattt tcgaagagta ttagaagaaa
    15001 aagaatgaaa gaaatagcct ttgacgcatt ttaccagctt taccaaaacg accagctttc
    15061 tctagtggat gtgagagaag tggatgagtt tgcagctctt catttagaag gtgcccacaa
    15121 cctaccgctt agtcaattga ctgatagtta tgattaattg gacaaagatc gcttgcatta
    15181 tattatttgc aaatctggaa tgagatcggc gcgtgcttgc caattcctgt tagaacaagg
    15241 ttataatgtt atcaatgtcc agggtggcat gttagccttt gaagaacttt aaaattttgc
    15301 atttctccta cttggtgtgg actgggtagg agagttttat ttttagataa ttcttatttt
    15361 taagaaaatt gaaaacattt aa
//
LOCUS       DAVLYR010000029        15334 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLYR010000029 DAVLYR010000000
VERSION     DAVLYR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.029"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            17..2182
                     /locus_tag="VYO63_000760"
     CDS             17..2182
                     /locus_tag="VYO63_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1671720.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2182..2922
                     /locus_tag="VYO63_000761"
     CDS             2182..2922
                     /locus_tag="VYO63_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1671721.1"
                     /translation="MVKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3071..4558
                     /locus_tag="VYO63_000762"
     CDS             3071..4558
                     /locus_tag="VYO63_000762"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1671722.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(4604..5893)
                     /gene="ftsY"
                     /locus_tag="VYO63_000763"
     CDS             complement(4604..5893)
                     /gene="ftsY"
                     /locus_tag="VYO63_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1671723.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVTEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5897..6715)
                     /locus_tag="VYO63_000764"
     CDS             complement(5897..6715)
                     /locus_tag="VYO63_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1671724.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLQAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6715..7509)
                     /locus_tag="VYO63_000765"
     CDS             complement(6715..7509)
                     /locus_tag="VYO63_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1671725.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(7506..11045)
                     /gene="smc"
                     /locus_tag="VYO63_000766"
     CDS             complement(7506..11045)
                     /gene="smc"
                     /locus_tag="VYO63_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1671726.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLEIILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEVIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11036..11734)
                     /locus_tag="VYO63_000767"
     CDS             complement(11036..11734)
                     /locus_tag="VYO63_000767"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1671727.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(11917..11988)
                     /locus_tag="VYO63_000768"
     tRNA            complement(11917..11988)
                     /locus_tag="VYO63_000768"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11953..11955),aa:Arg,seq:ccg)
     gene            complement(12052..13038)
                     /locus_tag="VYO63_000769"
     CDS             complement(12052..13038)
                     /locus_tag="VYO63_000769"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1671728.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13213..14529)
                     /locus_tag="VYO63_000770"
     CDS             complement(13213..14529)
                     /locus_tag="VYO63_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1671729.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14516..>15334)
                     /locus_tag="VYO63_000771"
     CDS             complement(14516..>15334)
                     /locus_tag="VYO63_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1671730.1"
                     /translation="VNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGG
                     KTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGE
                     KGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQ
                     VGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWS
                     DYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
BASE COUNT         4384 a         3441 c         2783 g         4726 t
ORIGIN      
        1 gaaaaggaga ctaaaaatga agaaaaaatt tctagcattt ttgctaattt tattcccaat
       61 tttctcatta ggtattgcca aagcagaaac gattaagatt gtttctgata ccgcctatgc
      121 accttttgag tttaaagatt cagatcaaac ttataaagga attgatgttg acattattaa
      181 caaagtcgct gagattaaag gctggaacat tcagatgtcc tatcctggat ttgacgcagc
      241 agtcaatgcg gttcaagctg ggcaagccga cgctatcatg gcagggatga caaagactaa
      301 agaacgtgaa aaagtcttca ccatgtctga tacttactat gatacaaaag ttgtcattgc
      361 tactacaaag tcacacaaaa ttagcaagta cgaccaatta actggcaaaa ccgttggtgt
      421 taaaaacgga actgccgctc aacgtttcct tgaaacaatc aaagataaat acggctttac
      481 tattaaaaca tttgacactg gtgatttaat gaacaacagc ttgagtgctg gtgccatcga
      541 tgccatgatg gatgacaaac ctgttatcga atatgccatt aaccaaggtc aagacctcca
      601 tattgaaatg gatggtgaag ctgtaggaag ttttgctttc ggtgtgaaaa aaggaagtaa
      661 atacgagcac ctggttactg aatttaacca agccttgtct gaaatgaaaa aagatggtag
      721 tcttgataaa attatcaaga aatggactgc ttcatcatct tcagcagtgc caactacaac
      781 tactctcgca ggattaaaag ctattcctgt taaggctaaa tatatcattg ccagcgattc
      841 ttcttttgca ccttttgttt tccaaaattc aagcaaccaa tacactggta ttgatatgga
      901 attgattaag gcaatcgcta aagaccaagg ttttgaaatt gaaatcacca accctggttt
      961 tgatgctgct atcagtgctg tccaagctgg tcaagccgat ggtatcatcg ctggtatgtc
     1021 tgtcacagat gctcgtaagg caacttttga cttctcagaa tcatactaca ctgctaatac
     1081 cattcttggt gtcaaagaat caagcaatat tgcttcttat gaagatctaa aaggaaagac
     1141 agtcggtgtt aaaaacggaa ctgcttctca aaccttccta acagaaaatc aaagcaaata
     1201 cggctacaaa atcaaaacct ttgctgatgg ttcttcaatg tatgacagtt taaacactgg
     1261 tgccattgat gccgttatgg atgatgaacc tgttctcaaa tattctatca gccaaggtca
     1321 aaaattgaaa actccaatct ctggaactcc aatcggtgaa acagcctttg ccgttaaaaa
     1381 aggagcaaat ccagaactga ttgaaatgtt caacaacgga cttgcaaacc ttaaagcaaa
     1441 cggtgaattc caaaagattc ttgacaaata cctagctagc gaatcttcaa ctgcttcaac
     1501 aagtactgtt gacgaaacaa cgctctgggg cttgcttcaa aacaactaca aacaactcct
     1561 tagcggtctt ggtatcactc ttgctctagc tcttatctca tttgctattg ccattgtcat
     1621 cggaattatc ttcggtatgt ttagcgttag cccatacaaa tctcttcgcg tcatctctga
     1681 gattttcgtt gacgttattc gtggtattcc attgatgatt cttgcagcct tcatcttctg
     1741 gggaattcca aacttcatcg agtctatcac aggccaacaa agcccaatta acgactttgt
     1801 agctggaacc attgccctct cactcaatgc ggctgcttat atcgctgaaa tcgttcgtgg
     1861 tggtattcag gccgttccag ttggccaaat ggaagccagc cgaagcttgg gtatctctta
     1921 tggaaaaacc atgcgtaaga ttatcttgcc acaagcaact aaattgatgt tgccaaactt
     1981 tgtcaaccaa ttcgttatcg ctcttaaaga tacaactatc gtatctgcta tcggtttggt
     2041 tgaactcttc caaactggta agattatcat tgctcgtaac taccaaagtt tcaagatgta
     2101 tgcaatcctt gctatcttct atcttgtaat tatcacactt ttgactagac tagcgaaacg
     2161 cttagaaaag aggattcgtt aatggtaaaa ttaaaaattg atgtaaatga tttacacaag
     2221 cactatggaa aaaatgaagt cctaaaagga attacgacta agttctatga aggagatgtt
     2281 gtttgtatca tcggtccttc aggttctggt aagtcaactt tcctccgtag cctcaatctt
     2341 ttagaagaag tcactagcgg tcacatcact gtgaacggct atgatttaac tgaaaaaaca
     2401 accaatgttg accacgtccg tgaaaatatc ggcatggtat tccaacactt caacctcttc
     2461 cctcatatgt ctgtattgga caacatcacc tttgctccta ttgagcacaa gttgatgact
     2521 aaggaagaag ctgaggaatt gggaatggag ttgcttgaaa aggttggact agcagataaa
     2581 gctaatgcca atccagatag cctatcaggt ggtcaaaaac aacgtgtggc catcgctcgt
     2641 ggcctagcaa tgaatccaga catcatgctc ttcgatgaac caacttctgc ccttgaccct
     2701 gagatggttg gagacgtact taacgttatg aaggaattgg ctgagcaagg catgaccatg
     2761 attatcgtaa cccatgagat gggatttgct cgtcaggttg ccaaccgcgt tatctttact
     2821 gcagatggcg agttccttga agacggaaca cctgaccaaa tctttgataa cccacaacac
     2881 cctcgtctga aagagttctt agataaggtc ttaaacgtct aaactcaaac tgtaaggatt
     2941 tccttgcagt ttttctacct cgtattggaa tttttgattt ttcggaaaat tatgttagaa
     3001 ttaagtttat gaaatgaggt ttcctcatac ctagcaagac taggaataaa aatagaaatt
     3061 aggtagctag atgtcatcta aggttattgt tacaattttc ggtgcgagtg gagacctggc
     3121 taaacgcaag ctctaccctt ccctttttag actatatcaa tccggcaatc tttccaagca
     3181 ctttgccgtt attggaactg cccgtagacc ttggagtaag gaatattttg aatctgtagt
     3241 tgtcgagtcc atccttgatt tggcagatag taccgagcaa gcccaagaat ttgctagcca
     3301 cttctactat caaagccatg atgtcaatga ttcggaacat tatattgctt tgcgtcaatt
     3361 acaagctgag cttaatgaaa aataccaagc tgaacacaat aagctcttct tcttgtctat
     3421 ggcacctcag ttctttggaa ccattgccaa acacctcaaa tctgaaaaca ttgtcgatgg
     3481 caaaggtttt gagcgcttga tcgttgaaaa accatttggt acagattacg caactgcaag
     3541 caagttgaat gacgaactcc tagcaacatt tgacgaagaa caaattttcc gtatcgacca
     3601 ttatcttggt aaggaaatga tccaaagcat ctttgcagtt cgctttgcaa acttgatttt
     3661 tgaaaacgtt tggaacaagg attttatcga caatgttcaa attacctttg cggagcgctt
     3721 gggtgtagaa gaacgtggtg gctactatga ccaatccggt gccctccgtg acatggtcca
     3781 aaaccacact ctacaacttc tttcgctcct cgccatggac aaaccagcaa gcttcacaaa
     3841 agacgagatt cgtgctgaaa agattaaggt ctttaaaaac ctctatcatc caactgatga
     3901 agaactcaaa gaacacttta tccgtgggca ataccgctct ggtaagattg atggcatgaa
     3961 atacatctct tatcgtagcg agccaaatgt gaatccagaa tcaacaactg aaacctttac
     4021 atctggtgcc ttctttgtag acagcgatcg attccgtggt gttcctttct ttttccgtac
     4081 aggtaaacga ctgactgaaa aaggaaccca tgtcaacatc gtctttaaac aaatggattc
     4141 tatctttgga gaaccacttg ctccaaatat tttgaccatc tatattcaac caacagaagg
     4201 cttctctctt agcctaaatg ggaagcaagt aggagaagaa tttaacttgg ctcctaactc
     4261 acttgattac cgtacagatg cgactgcaac tggtgcttct ccagaaccat acgaaaaatt
     4321 gatttatgat gtcctaaata acaactcaac taactttagc cactgggatg aagtttgtgc
     4381 gtcatggaag ttgattgacc gtattgaaaa gctctgggct gaaaatggtg ccccacttca
     4441 tgactataaa gctggaagca tgggacctca agccagcttt gacctacttg aaaaattcgg
     4501 tgccaaatgg acttggcaac cagatatcac ctatcgtcaa gatggtcgct tagaataaaa
     4561 aaatttcctg caagtttatg ccttgcagga tttttgcttc tgattagatt aaaccttcca
     4621 agagaccttt cataaagttt tctgagttaa actctccaat atcatcgatt ttttcaccaa
     4681 aaccaatcaa ttttacagga atattgagtt cttcacgaat ggctagaacc acacctcctc
     4741 gagcagttcc atcaatctta gtcaaaacaa ttcccgttaa aggtgtgatt ttcgaaaatt
     4801 ctttggcctg tactagggca ttttgacctg ttgatgcatc aagtgccaag aaggtttcat
     4861 gtggtgcttc tggcacaaca cgtttgataa tacgaccaat cttttccaac tcagccataa
     4921 ggttatcctt attttgcaga cgaccagcag tatcaatcat gagaatatcg ataccttcag
     4981 tcacggcacg ttccatacca tcaaagacca cgctggctgg atcagctttt tcaggtccag
     5041 ttactactgg aacatctact cgtcggcccc attcagctag ctgagctact gcacccgcac
     5101 ggaaggtatc tgctgcaacc agcatgacct tcttaccagc ttgtttgtag cggtgggcta
     5161 gttttccgat agaagttgtt ttcccaacac cattcacacc aacaaagagc ataactgtca
     5221 agttatcttg gaagtggatg ctttcatcgt agctaccatc cttttcataa agctcaacca
     5281 atttctcaat gatgacacga cgaagtacat caggtttctt ggcattttca agcttggctt
     5341 cgtaacgtag ttcctccgtt aagttagaag cgacttggac accaacatca ctcataatca
     5401 gcagttcttc cagttcctcg aaaaattctt cgtcaacaga gcggaagtta gcaaagaagg
     5461 cattcaagcg ggcaccgaaa cctgtgcgag ttttcttaag actgcggtca tatttttcct
     5521 gaacagtttc ttctgtttga ggagcttctg gttcaagcac ttcagaatta ttttcttcta
     5581 cagttccttc gtgctcaagc ttctcttcct ctggtaattc ttctgagttt ggtaattctt
     5641 ctatttcttc ttgagaaacc cctacagctg gctctgaatc ctgactttct tcaactgtgt
     5701 cttggatttc ctcttcttgg aacacagctt gttcaacaat ttcaacctct gcttcttcct
     5761 gagaaacttc ctcaacttct gtgaaggtag gatcaacatc ttcagacaaa tcaagatttt
     5821 ccagagcttc ttttacaact tcttcgattt taggttcttc tttttttccg aatagacggt
     5881 caaacaatcc catatcttag ttctccttta gcacatattc ttcgatagcc caggcgacag
     5941 cttcctcatc gttggtcatc ggcgtcacta catttgcggc tgcctttact tcaggaacag
     6001 cgttttgcat agcaacacca agacctgccc attcaatcat agagaggtca ttggcctcgt
     6061 caccacaagc catcacttga ctttggtcga ttccaagatg gctgattagt tttgccaaac
     6121 ctgttgcttt atgaacattc tttggtgacc attctagcaa catttcacgt gatttaaaga
     6181 tttcatattg gtcaaacaat tctggagaaa tcttctgaat ggctgcatcc aagggttctt
     6241 gagcaaaggc agtcacgcat ttgttgtagg tcatttgact agataagtct tcaaagtcca
     6301 ctggaacaaa ggtcaaagct ggattgaatt tggcataaag actttcttgg tccgattgga
     6361 tttgataaac tgttccttct gagatggcat caagaggcag tgataatttc tctgtttctt
     6421 catacaaacg tgccacatca tcatatgaaa agactgtttt atcaaggatt tctcctgtat
     6481 ttttctgaac taatccacca ttaaaagtaa tggtatactc atcttcctga ccgtcagtcc
     6541 ctaactcatg gagaaagaaa tccatggctt ttaagggacg accagttgtc aatacgacct
     6601 tgataccacg atcacgcgca gcttgcaagg tttccttggt acgatccgtc agccttttat
     6661 cagtagtcag caaggtcccg tccaagtcca atgcaatcaa ttttatatct gccattataa
     6721 gccctccata taagctataa ccgaccgttc cttatggtga ccaatcacag tctttgctaa
     6781 ttctaaaatt tcaggtcgtg cattttcagg agctacagga tgtcccacaa cctgcatcat
     6841 atgtaagtca ttaagattgt ctccaaaagc catgacctga tccattgtga taccaagttt
     6901 tttaactaat tcaacaatgg ccactccctt atcgacatag tccagaacaa tatcaatgga
     6961 ttcaaagcca gttgtcatgg ccttaacacc aggaacgttt tcgtttaccc aagcctcccc
     7021 atcttccagc gtttcttctg tgaagttggt tgtaaatttg aaaatgtcat ctgtgatatc
     7081 ttccaaactc gctacttttt ggatattttc attatagtgc tgactcactt tcaaataggt
     7141 ctcatcaacc gtatctagaa catatgaacc cttcttaccc gtcaagagca gtttattgat
     7201 atctacataa ggtgaagttt tcagcttttc aaaagttgcc agataaaagt cacgagacat
     7261 agtcgcttca tacaagtcct gaccttgata ctctaccaaa ctgccatttt ccgcgatgaa
     7321 aataatgtca tcacgaacac cagcaaataa tttttctaga gacagaaatc cccgacccga
     7381 agctaccgca aagtaaatcc ctttttcctt gtaggaaacc aagagagact tgagacgatc
     7441 catatcaaag cgtccattcc catctaggaa ggttccgtcc atatccgttg ctactagttt
     7501 aattgtcatc cttcaatact ttctaaatct tttaacttaa ctgaaacaat ctttgaaaca
     7561 cccgattctt gcatggtcac tccatagatg gaatcagccg ctgccatggt tcccttacgg
     7621 tgggttacga cgatgaactg gctgtccttg tcaaagcggt tgaggtaatc cccaaaacgt
     7681 ttaacattgg cttcatccag cgcagcttcc acctcatcca agataacaaa tggaatagtc
     7741 ttgacacgaa taatggagaa gagcaaggca agagccgata gggctttttc accaccactc
     7801 atgagattaa gagactggat tttcttgcct ggtggttgga cagaaatttc aaccccagct
     7861 gtcagcaagt ctccttcagt caaaatgagg tcagcctgac ctccaccaaa catctgcttg
     7921 aaggtcactt taaaggactc acgaatgacc tcaaaggttg atttaaagcg ttccttgacc
     7981 tcatcattca tctctgtaat ggtctcaagg agcaggtttt tcgcagacaa aatatcatca
     8041 cgttggctat ttaggaaatc cagacggttg tgaacttctt cgtactgttc aatagcgtct
     8101 aaattgacag gacccagtga gcgtatagcc ttctctaaat ccttaacttc ttgctctgcc
     8161 agattgagat tttccaactc atgcgccttt tctaaagctt ctgtgtagct gatctggtac
     8221 tggtctgtta attgactttg tagatggcgc aagcgctcgc taaccttttc tttcttggct
     8281 tcagcacgag tttgcttgcg aatccactct tcattctgct ggcgagcctg atccaaatga
     8341 ctagcaatat catccagttg accctcaata tcatccaact caaactgctt gcgaatcaaa
     8401 ccttgttgga gatttgtttt ttgagttttg gcttcatcag cctgttgact gagcaaatcc
     8461 gtatcaactt tctcaagatt gtcaaccttt tcttgaagaa ggcgctgaat ttcttcttgc
     8521 tcaatatcaa gattatctag ttccttacct aagcgttcaa tatcagcaac ttcataacgt
     8581 ttttgccctt gcagttctgt cttaagtaga cgagcttgag ctagctcttc ctgcaagttt
     8641 tgataacgtt cttggatggc gtttttatta gacttaatct cttcaatctc agcttccaga
     8701 ttattctttt cactagtgat tgtagtaagg cgctcttggc atttttcctt atccgcttgc
     8761 cattctccct cagaaagacg atttaattcc tcttcttgga gtttccaaag agtttctagc
     8821 tcttcaactt gctgactggt ctgctgatag gcgagggaca agccttgctc ctgaatacga
     8881 gcctgctctc cctgagattt gatggcttct aatctttctg tcaatctagc tagctcatct
     8941 tgcaagttct tcaaactcac ttcatctgaa cgcaaacttg cttcttcttc agcaatttct
     9001 ttttgtaatt gctccagttc tggcttgatg aaaatactgt tattctggcg attagctcca
     9061 cctgcataag aaccacctgt gcgcagctct gttccgtcca atgtcaccat acgaacctga
     9121 taacgaacct ggcgcgctgc tgcacgcgca tgctccacgg tatcaaaaat ggccgttgta
     9181 gctagcaaat tcttgaaaat ggcttccagt ctagtatcga aagtaaccag ctcatctgcc
     9241 atgcccagaa atcctggact tgcagcaata gtatcttggt tctgactaga aatcgtacga
     9301 gccttgatag tggtcaaagg aaggaaggtt gcacgaccag atctgtttcg tttgagtaaa
     9361 tcaatcgcct tggttgccga ctcttcatct tccacgatga tatgttggct actcgctcca
     9421 agcgcaatct ctagggcagt ttgataatgc acatcaaagg tcaggtgctc actaactgct
     9481 ccaataatcc cacctagacg atctttctct tggagaacac tcttaacacc tgcataaaag
     9541 ttactatgat ttctcaggat aatttccaaa ctttgcgctc tagcctgctt gtttttgaga
     9601 ctatccagac ggtcaaagag ttgactttgt tgagcttgat aggaagtttt ctgctcctct
     9661 tgctccttag cacagacttg atagtcggcc aataatttct gaacctgctc cttggcagtt
     9721 tcaagctcag ccttttgctg accagccttc tctttagctg tagccaactg ttctttcagc
     9781 ttttctagtt gatctgcttg tttttgagaa agctgacgac tattttccaa ctcattctca
     9841 atacgggtca gttggtttga gacatctgct tcttcttgta aaagggccac aaagcgttca
     9901 cgtaagagct caatcatctg atcaggatca tctgaaaaag ctagcaattc agcctctaat
     9961 cgattgagtt tttgattatt ttggactaga ttttcttcca agagggctaa agagctttct
    10021 ttatcagatt tttcttggct gagtgaactt ctcttatcct ccaaagtagc caaacgggct
    10081 tgtgcttcct gttgattgag ggctacttgc tcagattcca gtttcgatag ggctaatttt
    10141 ctctctaagt cactaatcag actggtcaaa tccatcaaac tgccttggtc tttggccatt
    10201 tcagcctgta aatcttggcg ttgcttttta agagtttgat tttcttcttc taatttttca
    10261 cgcttttggt aataactcat caagagttct tgaacctgag ccaactcttc ttctgtcgac
    10321 tctagttcag ccttattttc cttgatttga gcaaccagaa catctaaata aatagcctta
    10381 cgttgtcctt ccaagtctaa aaacttacgg gcattctcag cttgcttttc aagaggcttg
    10441 atttgattat ccaactcgta gataatgtcc tctaagcggt ccagattatc ctgagtttgc
    10501 tgcagtttac tctcggtttc ttttctgcga gtcttgtatt ttaaaactcc agcagcttct
    10561 tcaaaaatag ctcgtcgttc ctcaggcttg gaattaaaaa tctcctcaac cttcccttgg
    10621 gaaataatag agaaggaatc tcgtcccaat ccagtatcca agaagaggtc atgaatatca
    10681 cgcagacgga ctttcttgcc gtcaatcttg tattcgctat ctccactacg atagatatgg
    10741 cgttccaccc tgatttcttg acctgcatcc ttgataaatc cgtcatgatt atccagagtc
    10801 acaactacag aagcataatt gagcggtttg cgactttcgg ttccagcaaa gatgacatcc
    10861 ggcatcttgc ccccacggag actcttgaca ctagactccc ccaaagccca acgcagactt
    10921 tctgtaatat tggactttcc agatccattg ggtccaacaa ctgccgtcac accttggtca
    10981 aaaacgacct tggtcttatc agcaaaagac ttgaacccct gaatttcgat ttcctttaaa
    11041 tacatgaatc cagccccttc tcaacggcat ttttggcagc ttcctgctct gctaatttct
    11101 tagaacgacc ttggccttga ccgatgctct taccttcaac aagaacttct acatcaaaga
    11161 ccttatcatg agcaggccct gtttcagaaa tcacctgata acgaatagcc acatcaccat
    11221 tgacctgaag caactcttgg agatgggttt tatagtctgt aatcatctca aactcgcctg
    11281 cttcaacctt aggaatgatg acttgataga taaattcctt gaccttggcc acatccttat
    11341 ccaaaagaag ggcaccaaga aaggcttcaa aggcatcacc aagaatggtg tcacgattgc
    11401 gaccacctga tttttcttcc cctttaccca acttgataaa ctggttaaac tggcaatcac
    11461 gcgcaaaacc agctaaactc tcctcacgga caatcatagc acggagtttt gataggtcac
    11521 cttcaggctt tttaggatat tttttatata gatattctga aatcaataac tgtagaacag
    11581 cgtctcctaa aaattccaag cgttcattgt gtgaaatttt taagaggcgg tgctcattgg
    11641 cataactcgt atgagtaaag gcagtttcca gtaaattttt gtctgcaaat tcgattgcaa
    11701 aatgattctt tagtacagtt tgtaattctt tcataccaac ctctttctaa ctgataatag
    11761 tcctttttat tatatcaaaa aaagccccct gagtcactct aaaacgggac tggaaagcat
    11821 ttgggaattc tttagacaga gattctcagt tttagcggca aatttgggtc aggataaaga
    11881 aaaaagccct attaaaggct ttttaggatg tttacatcca ccctgaggga atcgaacccc
    11941 catctcaaga accggaatct tacgtgatat ccattacact aagggtggaa acttgtttta
    12001 ttataacaga aatttgctct aataacaagt tttttggtca aagaccccgt cttagtggga
    12061 agcatcccca ttccagatgg agtttttcac gatcacataa tcaacgtgtt taaggtcagc
    12121 aacctgacgt ccacctgcat aagaaatagc actttgaagg tcttgttcca tctcagttaa
    12181 agtgtcttgc agatgacctt tagcaggaag caagatacgt ttgccttcca catttttgta
    12241 agcacctttt tgatattgtg aggctgaacc ataatattct ttgaactgtt caccatcgac
    12301 ttcaatcgtt ttccctggac tttcaatgtg tcctgcaaag agggaaccaa tcatgatcat
    12361 gctagcaccg aagcggatag acttagcaat atcaccgtga gtacgaattc ctccatcagc
    12421 gataatcggt ttacgcgcag ccttggcaca ccagcgtaga gcagccaact gccaaccacc
    12481 tgtaccaaaa ccagtcttaa ccttggtgat acaaacctta ccaggaccga ttccgacctt
    12541 agtagcatcc gcaccagcat tttccaattc acgcacagct tctggtgttc ccacatttcc
    12601 agcaatgaca aaggtatctg gcaattcttt cttgatgtgt tgaatcatag aaatcacgct
    12661 atccgcatga ccatgagcaa tatcaatagt gatatactca ggagcatcag ccttgagctg
    12721 tctaacgaaa tcatactcat aatccttaac accgacagag atagaagcaa tgagcccttg
    12781 atcgtgcatt cgtttaataa aaggaatgcg tcctgcctca tcaaaacggt gcataatgta
    12841 gaagtaacca cctttagcca gttgctctgc tacattttca tccaaaatcg tctgcatatt
    12901 cgctggcaca acaggtagtt taaaggtgtg atttcctaaa gtgacacttg tatccgcttc
    12961 tgcacggctt ttaatgacac atttatttgg aatcaattga atatcttcgt aatcaaaaat
    13021 tggaaattca tttaacatat cgatgtctcg tttcttttgt aatgacctac ctatgctctt
    13081 gcatcactac gccttttccg acgtttcctt aatttattat aaaccaaagt acagtttttg
    13141 tcaaattatt ttatgaatta aaatatatcg ttcgggtttt acttgtgtta aaaataaaag
    13201 aaagagggga aattattttc tccccacttc ttcatcaata atcgctttaa aattttcttc
    13261 tgaattttcc atcattttac aaaatgtact atagaatgag gcattctgag ggattcgaat
    13321 agaccacttc ttcaattgag ctccatagcc agctactttt cctatttcac tatttactga
    13381 ctgctccata ctaggaaaaa tcagtccagc cttctcagct tttaaaatat aagaatagga
    13441 aatcagctgg aataagtcct cacggttgat tcctttttca gtcaattcca gttttttata
    13501 ttttgcatct agaacaatct ttcgttctct gtcataaaaa tctggatata cttttcgttt
    13561 cccaacagaa aatactgaaa ttccatccgt cttatcctta tttctgggat gtacaaaacc
    13621 ttttggcaac aaggtgtaaa catactcttc ccaaagccag gcaacatcaa agagaatacc
    13681 atagattttt tgatcttgat accctaaacc gtgcttttct tggtttagga tcatcagaca
    13741 aagttcttgt aagtttctgt actcgtgaaa gtatgcatga cgtataggtt tagattgatt
    13801 tccccgaata atcttagcac gatcagctag tttataagag ggcgttacac gcacgatttc
    13861 agatacgttt tcacgactag ttgagagatt atctagtacc ccttgaccaa tgcttttctg
    13921 attcttaatg tattcaatag tgtgacggac caactgcatg aggggattat cataggtgaa
    13981 ctctctcgtt gcgtaggcaa tatttcccgt gaaaggaaga tttttcttga gatggcttct
    14041 tacatcaatc actcccttaa cattactgtc gttatgagaa aatcgatgat attccttata
    14101 aagacctttt cgaatagcag cttgtagata cttgggaaag agatacacca aaagttgata
    14161 aagcctctct tcacgagaca aagcaacatc taaactagtg agattgatat gaagaacctt
    14221 gtttaagaga taatgcaaaa aatggtcatt actctcatca gaaaatcgtg aggaaatcgt
    14281 taatctttcc tgaccatatc caagaaaacc aatcacgttc cctgtcttga ttttctgatt
    14341 gactgtttca aaaatctttt ggtccttatc caaatcagga gtatgagtca aatcatttgg
    14401 aaaaatgaaa atacgttcat cttgggatag actttccaag gttctgtcta aaagaagatt
    14461 gcttagttta ggatattctt caacaaattt ttctttaata atcttgtgtt gattatcagt
    14521 aatccgcatc atcgttttca tcaccttcat tgtcatcagc tactgcctga tctttttgct
    14581 cattatttgt cagttcaaat gcttttttca aagtttccaa agtttcaacc tcatcataag
    14641 aacctcgcaa gtagtcttct aggagaggct taatataatc ggaccagagt aattcatagt
    14701 caaaatctac atccttcaac ttaagaaaat aacttggtcc aatatgataa tgactgttta
    14761 attcctgaac attttcgata gcagcgttca agtttcttag acgaattttt gcttcttctg
    14821 catggatatt cagctcttta tccaacatgc caacttgacc ctcggcagta acttcaacaa
    14881 aacgaaaacg acgacgcata gcaaaatcaa aggtatccac tgaacgatca atatcattca
    14941 tagttccgat gatgtaaaca ttttcgggga tatagaactt ttcatcagtt tcgtgtagat
    15001 ttgcatattg ggtagaaaca cttccttttt caccacgata gccagggtcg atagagaaaa
    15061 agagttcgcc aaaaatctta gaaatctccc cacgattgat ttcatcgatg atgaagacga
    15121 atttcttatc agtatcaatt tctgttgggg aaacatagtc tttcaaacca aatctctttc
    15181 tcaatgtttc taggacagtt tttccaccac ttttgtagta ttcttgctta ttataatttt
    15241 tatctttata caactcgtaa acatatttgc taggtagtga ccatcctgga acaccactat
    15301 cataatttac tgacaaattt tgtctactat taac
//
LOCUS       DAVLYR010000030        15197 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLYR010000030 DAVLYR010000000
VERSION     DAVLYR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.030"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..354)
                     /locus_tag="VYO63_000772"
     CDS             complement(<1..354)
                     /locus_tag="VYO63_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1671731.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINA"
     regulatory      383..479
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            477..986
                     /locus_tag="VYO63_000773"
     CDS             477..986
                     /locus_tag="VYO63_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1671732.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIQKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     gene            1086..1158
                     /locus_tag="VYO63_000774"
     tRNA            1086..1158
                     /locus_tag="VYO63_000774"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1119..1121,aa:Thr,seq:ggt)
     gene            1500..2576
                     /locus_tag="VYO63_000775"
     CDS             1500..2576
                     /locus_tag="VYO63_000775"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1671733.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            2632..3567
                     /gene="dapA"
                     /locus_tag="VYO63_000776"
     CDS             2632..3567
                     /gene="dapA"
                     /locus_tag="VYO63_000776"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1671734.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(3614..4987)
                     /gene="mnmE"
                     /locus_tag="VYO63_000777"
     CDS             complement(3614..4987)
                     /gene="mnmE"
                     /locus_tag="VYO63_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1671735.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(5124..5306)
                     /locus_tag="VYO63_000778"
     CDS             complement(5124..5306)
                     /locus_tag="VYO63_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1671736.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            5430..6029
                     /locus_tag="VYO63_000779"
     CDS             5430..6029
                     /locus_tag="VYO63_000779"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1671737.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPIMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            6019..6498
                     /locus_tag="VYO63_000780"
     CDS             6019..6498
                     /locus_tag="VYO63_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671738.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIVISSGNYLLFCFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            6508..7587
                     /gene="prfA"
                     /locus_tag="VYO63_000781"
     CDS             6508..7587
                     /gene="prfA"
                     /locus_tag="VYO63_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1671739.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLKELNK"
     gene            7587..8426
                     /gene="prmC"
                     /locus_tag="VYO63_000782"
     CDS             7587..8426
                     /gene="prmC"
                     /locus_tag="VYO63_000782"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1671740.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     RQD"
     gene            8410..9012
                     /locus_tag="VYO63_000783"
     CDS             8410..9012
                     /locus_tag="VYO63_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1671741.1"
                     /translation="MIDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            9014..9445
                     /locus_tag="VYO63_000784"
     CDS             9014..9445
                     /locus_tag="VYO63_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671742.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSLEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGEGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            9507..10763
                     /locus_tag="VYO63_000785"
     CDS             9507..10763
                     /locus_tag="VYO63_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1671743.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            10771..11745
                     /locus_tag="VYO63_000786"
     CDS             10771..11745
                     /locus_tag="VYO63_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1671744.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            11745..12359
                     /gene="pmp23"
                     /locus_tag="VYO63_000787"
     CDS             11745..12359
                     /gene="pmp23"
                     /locus_tag="VYO63_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1671745.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            12433..13407
                     /locus_tag="VYO63_000788"
     CDS             12433..13407
                     /locus_tag="VYO63_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1671746.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            13481..15112
                     /gene="rlmD"
                     /locus_tag="VYO63_000789"
     CDS             13481..15112
                     /gene="rlmD"
                     /locus_tag="VYO63_000789"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1671747.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKIFMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
BASE COUNT         4654 a         2899 c         3256 g         4388 t
ORIGIN      
        1 agcatttatc ttatcaatgt tttcagttac gaattgccct ataatgtgag gaaattgggt
       61 tacaaagaga aaagttaaaa acaagtctaa cagaaaagta gaaaggcgag caagagaaaa
      121 tttcactaaa cggataagtc tatttttctt tttttgctta aatacaatcg tatcatttgt
      181 gataaaggca aataggatac cgataatatt tgctagggca gtagccagga gttcctgatg
      241 acttagttga taaatgacta gtcgtgagag aatcgaaact agagttgtcg caaggccaaa
      301 aaagagatag gctaagattt cattgttaaa aaatttttga ataggaattt tcatgtgtta
      361 agtatagcat aaagcaggct attgtgctat actagtaggg ttgaataaag caacccttgg
      421 tgcttagctt ctttcaccaa gcatattaca cgcgggcaac cgccaaagga gaaaagatga
      481 aaaaattaac tattcgtgat gttgcagata ttgcaatcgt cgctgctatc tacgtcgttt
      541 tgactgtcac accgccccta aatgccatca gctatggtgc ttatcagttt cgtatttcag
      601 aaatgatgaa ctttatggct ttttacaatc ctaagtacat catcggagtt accatcggtt
      661 gtatgattgc taatttcttt agcttcggac tgctagatgt ctttgttggt ggtggttcaa
      721 ccttagtatt cctcagttta ggtgtttggc tttttgcaaa atatagcaag gattacctct
      781 ttaacggatt gattcaaaaa gatcatttct tcttttcaat cctcttttca atttccatgt
      841 ttactatcgc tgcagagtta aatattgtag cagaattacc attcttcctt acttggttta
      901 caacaggaat cggtgaattt gcttcattga ttattggggc gattattatt ggtaaaattg
      961 gtcgtcgaat tgatttaagt aaatagaaga agataagcta ggtagttctt acctggcttt
     1021 ttcctatgga aaatttctaa ggaaacttga caagtttttc tgaatatagt atacttctta
     1081 agtaagctga tttagctcag ttggcagagc gcatccatgg taaggatgag gtcgccggtt
     1141 cgatcccggc aattagcatg atatgtatag ggaaactctt gaatacgaga gttttttgtt
     1201 atactcgatc gaaaaaattt cttattgaca aaatcaagta tctctagtaa tatagaatat
     1261 gcgatgagtc gataggtagt cttcggacta ctattgagca taaggaggtc ataacgcagg
     1321 agcggacctt gatgagttgt gtgaacctgc tcatcacatg aagatgcctc ttagtcccta
     1381 gtctagcgac tgggattttt attttccaaa aaatgatgaa aaagctttgc aattcatgga
     1441 aaaagtagta taatacttct attatagaaa tttttagaaa attccgaaag aggttattta
     1501 tgggatatac agttgctgta gtcggcgcga caggtgctgt cggtgctcag atgataaaaa
     1561 tgttggaaga atcaacactt ccaatcgata aaatccgtta ccttgcttct gcacgttcag
     1621 caggtaagtc attgaaattt aaagatcaag atattacaat tgaagaaacg actgaaacag
     1681 cttttgaagg agttgatatt gctctctttt cagcaggtag ttctacatca gctaagtatg
     1741 caccatacgc agtaaaagct ggcgtggtag tagtggataa tacatcttat ttccgtcaaa
     1801 atccagatgt tcctttggtt gttccagagg tcaatgctca tgcacttgat gcccacaacg
     1861 gaatcattgc ctgccctaat tgttcaacaa tccaaatgat ggtggctctt gagccggttc
     1921 gccaaaaatg gggcttggac cgtatcattg tttcaactta tcaagccgtt tcaggtgctg
     1981 gtatgggagc aattcttgag acacaacgtg aacttcgtga agtcttgaat gatggtgtga
     2041 aaccatgtga tttgcatgcg gaaattttgc cttcaggtgg tgacaagaaa cattatccta
     2101 tcgcctttaa cgctcttcca caaattgatg ttttcactga taatgattac acgtacgaag
     2161 agatgaagat gaccaaggaa actaagaaaa ttatggaaga tgatagcatt gcagtatctg
     2221 caacatgtgt gcgtattcca gtcttgtcag ctcactctga gtctgtttat atcgaaacaa
     2281 aagaagtggc tccaatcgaa gaagtaaaag cagctatcgc agccttccca ggtgctgttc
     2341 ttgaagatga tgtagctcat caaatctatc ctcaagctat caatgcagtt ggttcgcgtg
     2401 atacctttgt tggtcgtatc cgtaaagact tggatgcaga aaaaggaatt cacatgtggg
     2461 ttgtttcaga taaccttctc aaaggtgctg cttggaactc agttcagatt gctgaaactc
     2521 ttcatgaacg tggattggtt cgtccaacag ccgaattgaa atttgaatta aaatagtcat
     2581 atcgtttagg agttcagatg aactccttct ttgaaataga gaggtgtttt catgtcttat
     2641 caagatttaa aagaatgtaa aatcattaca gcctttatta ccccctttca tgaggatggt
     2701 tctattaact ttgatgctat tccagccttg attgagcatt tattggccca tcatacggat
     2761 ggaattcttc tcgcaggaac gactgctgag agtccaactt tgacccacga tgaggagttg
     2821 gagttgtttg cggctgtaca aaaggttgtc aatggacgcg ttcctttgat tgcgggtgta
     2881 ggtactaatg atacgcgtga ctctattgag tttgtcaaag aagtagcgga atttggtggt
     2941 ttcgcagctg ggcttgctat tgttccttac tacaacaaac cttctcaaga agggatgtat
     3001 cagcacttta agactattgc agatgcttct gacctaccaa ttattatcta taacattcca
     3061 gggcgtgtag ttgtcgaatt gactccagaa accatgcttc gcttggctga ccatccaaat
     3121 attatcggtg tcaaagaatg tactagcttg gctaatatgg cttacttgat tgagcacaag
     3181 cctgaagagt tcttgattta tacaggtgag gatggagatg ctttccatgc catgaacctt
     3241 ggggcggatg gggttatttc tgttgcctct catacaaatg gggatgaaat gcacgagatg
     3301 tttactgcca ttgcagaaag cgatatgaag aaagccgcag caattcagcg taaattcatt
     3361 cctaaggtta atgctctctt ctcttatcca agtcctgctc cagttaaggc aattcttaac
     3421 tatatgggat ttgaagctgg acccactcgt ctacctcttg ttccagcacc agaagaagat
     3481 gccaaacgca ttatcaaggt tgtcgtagat ggcgactacg aagcaactaa ggcaactgta
     3541 acaggggtct taagaccaga ttactaataa agacaataaa atccatgacc gaatgaagga
     3601 tcatggattt ttcttatttt cctaaacaga attggctaaa gagttgggtg atgagttcat
     3661 ctggagcagc atccccagtg atttctccga ggatttccca agtacgagtc aagtcaactt
     3721 gaagcaaatc aactggcatc cccagctcaa gaccttgatt aacggcttgt aggctttcaa
     3781 ctgctttttc aatcagggaa atgtgacggg cgtttgacaa gtaagtagca tcttgctcaa
     3841 tcaagccagc attttcaaag aagaggttgt tgattcgctc ttcaatcttg tcgatgtttt
     3901 ggtttttaag gactgaaata cggataacgt cttcaggtag tttcgaagtt tcaatcgttt
     3961 ctggcaggtc ggttttatta agtagaataa tgcgattggt atcttggcta atttcaagaa
     4021 gttgtctgtc ttgcgcagtc agtggttcac tggcatttag cactagtaga accaagttgg
     4081 cttccttgag ggcttttttc gaacgctcaa caccgatttg ttccactata tcatccgttt
     4141 cacgaatacc agctgtatca atcaatttga gaggtacacc attgatgttg acgtactctt
     4201 cgatgacatc tcgtgttgtc ccagcgatat cggttacgat agccttgtcc tcacgcaaga
     4261 ggttgttgag aaggcttgat ttcccaacgt tgggacgtcc aatgatagcc gttgaaattc
     4321 cttcacgaag aattttacca cgacgtgctg tcctaaggag cttggttagt aattgctcaa
     4381 actccattgt cttctctcgg acaacagcag tagtggcttc ctcaacatcg tcatactcag
     4441 gatagtcgat attgacctca acttgggcaa gtgtgttaag gatttcttga cggatattat
     4501 tgataagatc agaaagggaa ccatcaagtt gtttgaccgc gatgttcatg gccttgtctg
     4561 tcttggcgcg gatgatatcc atcaccgcct cggcctgtgt caaatctacg cgaccgttta
     4621 aaaaggcacg tttggtaaat tcaccaggtt ctgccaaccg agctccttca cggatagcta
     4681 gctggagaat ctcattggtc accgcaatac caccgtgggt gttaatctcg ataatatcct
     4741 cacgagtgaa ggtctttgga gacttcatag ccccaaccat aacctcgtcc atgactttac
     4801 cagtcagagg atcaataatg tgaccgtagt tgagagtgtg gctggcaacc ttgttcaagt
     4861 cttttccttt aaaaatcttt tgcgcaatag caaaactgtc tgttccgctc aggcggacaa
     4921 taccaatagc cccttcacct agtggagtag agatagcagc gatggtatca aattcacgtg
     4981 taatcataat gtttccttta attaatagct attttcttgg aaattctcca agtttctttt
     5041 caaattgtaa caaatttagg atatttcttc aatgaatgct acttggagat tgaaaaagcc
     5101 taagcaattc tgcttaagct agcttatttg gtacgcattt ccccttgtgg gaagtaagtt
     5161 ccttctggca tgtcgttgat gatgacatgg acagcagatt gaggtgctcc agtgttgcga
     5221 acaaccgctt ccgttacttc cttagcaaga gctttctttt gctcgagcgt gcgtccttca
     5281 aataaatcga tgcgtacaaa tggcataata gcttcctcca ctagtttttg atttcttcta
     5341 ttttaccaca ttttgccgtt taaagcttaa gaaaattatg atatactaga atgtagcaaa
     5401 aatttagaaa tggacgtgaa gcaagaaaca tggcacagtt gtattatcgt tatgggacca
     5461 tgaactctgg aaagacaatc gagattctca aggtggccta taactatgag gagcaaggaa
     5521 aaggtgttgt cattatgacc tcggctctgg atacgcgtga cggtgttggc tatgtgtcga
     5581 gtcgaattgg catgaaacgc cctgcccttg cgattgagga aacaacggat atctttggct
     5641 atatccgaga cctaccagaa aaaccttact gtgttttggt cgatgaagcc cagtttctta
     5701 aacgccacca tgtttacgac ctagctcgtg ttgtggacga gttagacata cccatcatgg
     5761 cttttggttt gaaaaatgac tttcgcaatg aattgttcga aggttccaaa tatctcttgc
     5821 ttttagcaga caagattgac gaaatcaaga ccatctgtca gtattgcaag aaaaaggcga
     5881 ccatggtgtt gcgaacacag gatggactgc ccgtttacga tggagaacag atccagattg
     5941 gtggtaatga aacctatatc tcggtttgcc gtaaacatta ttttgcgcca atgataacct
     6001 ctaacaagga gcaaaactat gacaattgaa ctaagagatg ttacaatgga aaattatttt
     6061 gatgttttaa atttggatgt caaggaatat caaaaacaat tcattgcaac caacgcaatt
     6121 agtttagctg aagcatacgt ctacactaaa aatggagatt ttgtagctcc attggcagtt
     6181 tatgataatg atgtaattat aggttttgtg atgatagctt atgataaaaa gatcgtaatt
     6241 agtagtggaa attatttact attttgtttt atgattgata agaattttca aaatcaagga
     6301 tattttaaac caattatgga taaagtacta gactatgttc gaacagcgcc agcaggttta
     6361 agcaataaac tttggttgtc ttatgaacca gaaaatgaac aggcaagatt ttgttatctc
     6421 agttatggat ttaaagaaac tggggaaata tccgagaatg aagtagtagc aatctatgat
     6481 ttaacaattg agaaataagg agaaaaaatg aacatctatg atcaactaca agctgtagaa
     6541 gaccgttatg aagaactagg agaattgctg agtgaccctg atgtcgtttc agacaccaag
     6601 cgttttatgg agctttcaaa agaagaagct tccaatcgtg acaccgtaat agcctaccgt
     6661 gagtataaac aagtccttca aaatatcgtc gatgccgaag agatgattaa ggaatcaggc
     6721 ggagatgcgg acttggaaga aatggccaag caagaactca aagatgccaa ggctgaaaaa
     6781 gaagaatatg aagaaaaact gaaaattttg ctccttccaa aggatccaaa cgatgacaag
     6841 aatatcatcc ttgaaatccg tggagcagct ggtggagacg aagcggcact tttcgctgga
     6901 gatttgctaa ctatgtacca aaagtatgcg gaagcccaag gttggcgctt tgaagtcatg
     6961 gaagcctcta tgaatggtgt cggtggtttt aaagaagtgg ttgctatggt ttcaggtcag
     7021 tctgtatact ctaagcttaa gtatgaatca ggtgcccacc gtgtgcaacg tgttcctgtg
     7081 acagaaagcc aaggccgtgt tcatacttcg acagcgacag ttcttgttat gccagaagtt
     7141 gaagaggttg aatacgacat tgatccaaaa gaccttcgtg tcgacatcta tcacgcctct
     7201 ggtgctggtg gacagaacgt caataaggtt gcgactgccg ttcgtatcgt tcacttgcca
     7261 accaatatca aggttgagat gcaggaagaa cgtacccagc agaagaaccg cgagaaggct
     7321 atgaagatta tccgtgcacg cgtcgctgac cactttgctc agattgctca ggatgaacaa
     7381 gacgctgagc gtaagtcgac aatcggtact ggtgaccgtt cagaacggat ccgaacttat
     7441 aacttcccac aaaaccgtgt cacagaccac cgtatcggct tgaccctcca aaaactagat
     7501 acgattttgt ctggtaaatt ggacgaagtt gtggatgcct tggtgcttta tgaccaaaca
     7561 caaaaactaa aagaattaaa caaataatga aattagctca attattttca aattttgaag
     7621 aagagttgat aagacaagga gaggaagcgg aaagcctctc ttttgtctat cgtagtctga
     7681 aaaatctatc ttttacagac tttatttttg ccctccagca ggaggtgaca acagaggaag
     7741 aaaaacaatt tgtagaagat atttaccagc aactagcagc tcacaagcca gctcagtaca
     7801 tcattggaca ggcagatttt tatggaatgc atttaaaggt ggatgaacgg gttttgattc
     7861 ctcgtccaga aacagaggag ttagtggagc ttattctaac tgaaaatctt gagacgaatc
     7921 tttcagttct ggatattgga acaggtagtg gagctattgc tctcgctcta gcaaaaaata
     7981 gaccagattg gtcagtgaca gcagcagatg tttctcaaga ggccttagag cttgcatcag
     8041 agaatgctag caatcaaaat cttaatatat tttttaaaaa atctgattgt tttgcagaaa
     8101 tttctgaaaa atatgatata attgtatcca atccacccta tatctctcgt gaagatgagt
     8161 cagaggtagg cttgaatgtt ttgcattcgg agcctcatct agctctcttt gcagacgagg
     8221 atggcctagc tatttactgt agaattgcgg aagatgcaaa agactatctc aaagatggtg
     8281 gtaagattta ccttgaaatt ggatacaagc aaggtcaaag tgttcccgaa ctttttagga
     8341 aacatcttcc tgaaaaaaga gttcgaacac tcaaggacca atttggtcaa aataggatgg
     8401 tcgtggttga tgatagacag gattagacaa gagttggaaa atggtggagc tgtcgttcta
     8461 cctacagaga ctgtttatgg tctttttgcc aaggccttag atgaaaaagc agttgaccat
     8521 gtttaccaac tcaaacgtcg tcctagagat aaggcactca atctcaatat cgcctctttc
     8581 gaggatatct tgcacttttc taagaatcag ccaacttatc tacaaaaact tgtagagacc
     8641 tttttgccag gtcccttgac cattattctc gaagccaatg accgagttcc ctattgggta
     8701 aattctgacc ttgcaactat tggatttcgg atgcccagtc accctatcac actggattta
     8761 attcgagaga caggtccctt gattgggccg tctgccaata tctcaggtca ggcaagtggt
     8821 gtaacctttg aacaaattct gaaggatttt gaccaagagg ttctgggtct ggaagacgat
     8881 gcttttctaa ctggacagga ttcaactatt gtggatttgt ctggagacaa ggtgaaaatc
     8941 ttacgccaag gcgcaattaa acgagaagat attcttgctc ggttgccaga gatttctttt
     9001 gaggaggctt gaaatgctaa gagatttgca agaaacagat gtgaaagcga tatgtgacat
     9061 caaccaagag gctttgggtt atacttttag tctagaggaa acggctagcc aactagctag
     9121 actgtctcag gattcccatc atttcctact tggctatgag gatgcagcta atcatgtctt
     9181 acttggatat gtccacgctg aagtttacga atcactctat tccaaagcag gatttaatat
     9241 cttagcttta gcagtttcac ctcaagcgca aggtgaaggt atcggtaaaa gtttactaca
     9301 agggttggaa caagaagcca aaagatgtgg ttatgggttt atccgcttaa attctgccaa
     9361 tcatcgtctg ggtgctcatg cattttatga aaaagttggc tatacttgtg ataaaatgca
     9421 gaaacggttt attcgcatct tttagtttga ttttcttatt gtaaaatcaa actaatggac
     9481 tagtcacaca ataaaggaga agacctatga tttttgacaa agatgatttt aaagcatatg
     9541 atgctgatct ctggaatgct attgccaaag aagaagaacg ccaacaaaat aatatcgagt
     9601 taattgcttc ggaaaacgta gtttccaagg ctgttatggc agctcaaggg tctatcttga
     9661 caaataaata tgccgagggt tacccaggac gccgttatta tggtggaact gatgtagtag
     9721 acgttgtaga gactcttgct attgaacgcg caaaagaaat tttcggtgct aaatttgcca
     9781 atgtccaacc acattcagga agccaagcta actgtgcggc ttacatgtcc ttgattgagc
     9841 caggtgatac ggttatggga atggatttgg catcaggtgg tcatttgact catggggctc
     9901 ctgttagctt ctctggtcaa acctacaact ttgtttctta tagtgttgat cctgaaacgg
     9961 aactcttaga ctttgatgct atcttgaaac aagcccaaga agtaaaacca aaactgattg
    10021 tagctggtgc ttcagcatat tctcaaatta tcgatttttc aaaattccgt gaaatcgcag
    10081 atgctgtcgg tgcgaagctc atggtggaca tggcccatat cgctggtttg gttgcggctg
    10141 gccttcatcc aagcccagtt ccatacgctc atatcacaac aacaacgacc cacaaaaccc
    10201 ttcgtggtcc tcgtggtggt ttgattttga ccaatgacga agaacttgct aaaaaaatca
    10261 attcagctat tttcccaggt attcagggcg gtcctttaga gcatgttgtg gcggctaagg
    10321 cagtttcctt caaagaagtt ttggatccag ccttcaagga atatgctgcc aatgtaatta
    10381 agaacagcaa ggctatggca gatgtcttct tgcaagaccc tgatttccgt attatttcag
    10441 gtggaactga aaaccatctc ttccttgttg atgtgactaa agttgtagaa aacggcaaag
    10501 ttgctcaaaa cttgttggat gaagtcaata ttaccttaaa taaaaactca atcccttacg
    10561 aaagcttgtc accattcaag acaagtggga ttcgtatcgg agcagcagcc attactgcac
    10621 gtggatttgg tgaagaagaa agtcgcaaag tggctgaact catcattaaa acccttaaga
    10681 attcagaaaa tgaggctgta ttagaagaag tgagaagtgc agtcaaagaa ttgacagatg
    10741 ccttcccatt atacgaggaa taaaactttt atggacattt atattaagaa agccattatt
    10801 caccagttca gtccggatga taccgagctg ttcttagcag ataagtttct caatattact
    10861 ccaaaaatcg aagaatacct acgtaaaaaa attgaacatg tgtattcaga tgaagccaag
    10921 actgggattt tcgaagaaga aaatcccttc ttcaatcata ttacagacga tttgttggag
    10981 acatcagtaa cgctggctaa tctctggaaa gaggagttta gcatttctga aaatctcaag
    11041 accaatgact tgatttttgt tcaattttct aaagaaggtg tagaacattt cgctttcttg
    11101 cgaattgccc tgcgggagac cttgacccac ctcggaggag aagttgataa tccaatcaag
    11161 ctgactcaga ataacctgcc tggatttgga acgggtgctg acgaggcctt ggtggtcaat
    11221 cttcagagtc gcaagtatca cctgattgaa aaacgaatca agtacaacgg gacttttttg
    11281 aactattttt cagataatct tcttgctgtc gctcctaaga tttctcctaa aaaatctatc
    11341 aaggaactgg aaaaaacagc ccagagaatt gctgaatctt ttaacacaga tgattttcaa
    11401 tttcaatcca aggtcaaatc agctattttc aacaacctag aagaaagcaa tgaattgtca
    11461 cctgagaaat tggctaatga cctttttgac aacaatctga cggctcgttt gagctttatt
    11521 gaccaagtca aagaagccgt accagaacct gttcaatttg atgaaattga tgccagtcgc
    11581 caattaaaga aatttgaaaa ccaaaaactc tccttatcaa atggaattga gctcatcgtt
    11641 cccaataacg tctatcaaga cgccgagtct gttgagttta tccaaaacga aaatggaacc
    11701 tactctatct taatcaaaaa tatcgaggat atccaaagta aataatgttt aaacgaattc
    11761 gaagagtgct tgtactagca gtcttccttt ttgctggcta taaagcttac cgcgttcatc
    11821 aagatgtcaa acaagtcatg acctatcaac ccatggtgcg agaaatattg agtgaaaaag
    11881 acaccccagc aaacgaagag cttgtgcttg ctatgattta tactgaaaca aaaggaaaag
    11941 aaggcgatgt tatgcagtct agtgagtctg caagtggttc caccaacacc atcaatgata
    12001 atgcctctag cattcggcaa ggcattcaaa ctctgacagg caatctctat ctggcgcaga
    12061 agaagggtgt agatatctgg acagctgttc aagcctataa ttttggacct gcctatatcg
    12121 attttatcgc ccaaaatggc aaggaaaata ccttggctct agccaaacag tactctcgtg
    12181 agactgttgc ccccttgctt ggtaatacga ctggaaagac ttatagttat attcacccca
    12241 tttccatttt tcacggtgct gaactctatg taaatggagg aaactattat tattctagac
    12301 aggtacgact taacctttac atcatcaaat gtttcactct cttttcaaca tctggctaga
    12361 ccaggtgttt ttgttataag ttttctttaa gatagatata ttactctaaa gaagtaaagg
    12421 aggaaattcc ctatgagaaa gaaactcttt ctgactagcg ctgcgatctt gtgggcagta
    12481 acagctatga atagcgtcca tgcagcaaca gatgttcaaa aagttatcga tgaaacctat
    12541 gtccaacctg aatatgtcct aggttcttcc ctatctgaag accaaaaaaa tcaaactctt
    12601 aaaaaactgg gctacaatgc ctcaacagat accaaagaac tcaagaccat gacacctgat
    12661 gtttattcta aaatcatgaa tgtggccaat gactctagct tacagttgta ttcatcagcc
    12721 aagattcaaa agctaggtga caaatcgcca cttgaggtca agattgaaac acctgaaaac
    12781 atcactaagg taactcagga tatgtaccga aatgcagccg taactttggg tgtggaacac
    12841 gccaaaatca ctgtagcagc acccattcca gttacaggtg agagtgcttt ggctgggatc
    12901 tactattcgc tagaagctaa tggagccaag gtgccgcaag ccaataaaga tttggctcaa
    12961 gaagagctaa aggctttgtc agatatcaat gctgaaaaca aggacaaatc aggctatgat
    13021 gctaataaat taaacgttgc cctagctgat atcaagtcag gactcgccaa agctaaagaa
    13081 agcaagggaa atctgacaga agaagatatc cgcaagattg ttgaagatac cttaaaaaat
    13141 tacaaacttg atcaggtcat aacaggaaac cagatcaata tcatcatcaa ttttgccttg
    13201 aatctctcaa agagtgatat cctcagcaat gcagatttca ctaaaaccct aaatgacctt
    13261 aaacaaagca tcgtatcaca agctggcgac agttttaaaa atatcaacct taactttgat
    13321 gcggataagg cgctagagga cggtggtaac ttcttaagct ccctctggca agcccttgtc
    13381 aacttcttca agagttttgg ttcttaagaa atttcatggt ataatggatg gtaaccgtaa
    13441 cgttgccgtc ttttttgtcg gccaatagaa agagaaaaga atgttaaaga aaaatgatat
    13501 tgtagaagtt gaaattgttg atttgaccca tgaaggggca ggagttgcca aggtagatgg
    13561 tttggtcttt tttgtagaga atgctttacc gagtgaaaaa attttcatgc gtgtcctcaa
    13621 ggtcaataaa aagattggct ttggaaaagt tgaaaaatac cttgtccagt caccacaccg
    13681 taatcaagat ctagatttgg cttacctgcg ttcaggaatc gcggatttag gacacctttc
    13741 ttatccagaa cagctcaagt ttaaaaccaa gcaagtcaag gacagtctct acaagattgc
    13801 tggaattgca gatgtagaag ttgctgaaac gcttggtatg gaacatccag tcaagtatcg
    13861 caataaggcg caggtgcccg ttcgtcgagt gaatggtgtc ttggaaacag gatttttccg
    13921 taagaattcg cataacctca tgccccttga agatttcttt atccaggatc ctgtcattga
    13981 ccaagtcgta gtagctcttc gagacctgct ccgtcgtttt gatttaaaac cttatgacga
    14041 aaaggaacag tctggattga ttcggaatct tgtggtgcgt cgtggtcact attcaggaca
    14101 aatcatggtc gttttggtga caactcgtcc aaaagttttt cgtgttgacc aattgattga
    14161 acaagttatc aagcagttcc cagagattgt gtctgtcatg caaaatatca acgaccagaa
    14221 taccaatgcg atttttggta aggagtggcg cactctttat ggtcaagact atattacgga
    14281 ccagatgttg ggaaatgact tccaaatcgc tggcccagcc ttttaccaag tcaatactga
    14341 aatggcggag aaactctatc aaacagccat tgactttgca gagttaaaaa aagatgacgt
    14401 ggttattgat gcttattctg gtattggaac cattggttta tcagtcgcca agcatgtcaa
    14461 agaagtctac ggtgttgaac tgattccaga agcggttgag aatagtaaaa aaaatgctca
    14521 gctgaacaat atttcaaacg cccactatgt ctgtgacaca gctgaaaatg ctatgaagaa
    14581 ttggcttaaa gatgggattc aaccaaccgt tatcttggtt gatcctccac gcaagggctt
    14641 gacagaaagc tttatcaaag caagcgccca aacaggagcc gatcgcatcg cctatatctc
    14701 ctgcaatgtc gcaaccatgg cgcgtgatat caaactctac caagaattgg gatatgaatt
    14761 gaagaaagtc cagccggtgg atctatttcc tcaaacgcat cacgtcgaga cggtagcact
    14821 tttgtccaaa cttgatgtcg ataagcacat aagtgttgaa attgagctgg atgagatgga
    14881 tttgacaagt gcggagagca aagcaacata tgctcaaatc aaagaatatg tttggaataa
    14941 atttgaatta aaagtttcga cattatatat tgcacagata aaaaagaaat gtggaataga
    15001 attacgagaa cattacaaca agtctaaaaa ggataaacaa attattccac agtgtacacc
    15061 tgaaaaagaa gaagccatca tggatgcttt gagacacttc aaaatgattt aatagaaaag
    15121 aatgacagta tatgactttc tgcatttatt acattcctac ttggtatagg aacagctatt
    15181 attcctttct tgcaagg
//
LOCUS       DAVLYR010000031        14950 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLYR010000031 DAVLYR010000000
VERSION     DAVLYR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14950
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.031"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            149..496
                     /gene="ssrA"
                     /locus_tag="VYO63_000790"
     tmRNA           149..496
                     /gene="ssrA"
                     /locus_tag="VYO63_000790"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(669..756)
                     /locus_tag="VYO63_000791"
     tRNA            complement(669..756)
                     /locus_tag="VYO63_000791"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(720..722),aa:Ser,seq:gga)
     gene            919..1659
                     /locus_tag="VYO63_000792"
     CDS             919..1659
                     /locus_tag="VYO63_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1671748.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1656..2567
                     /gene="pfkB"
                     /locus_tag="VYO63_000793"
     CDS             1656..2567
                     /gene="pfkB"
                     /locus_tag="VYO63_000793"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1671749.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2564..4516
                     /locus_tag="VYO63_000794"
     CDS             2564..4516
                     /locus_tag="VYO63_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1671750.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSAGQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4680..6905
                     /locus_tag="VYO63_000795"
     CDS             4680..6905
                     /locus_tag="VYO63_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1671751.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKPLLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(6950..7378)
                     /locus_tag="VYO63_000796"
     CDS             complement(6950..7378)
                     /locus_tag="VYO63_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671752.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITAGITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7516..8658
                     /locus_tag="VYO63_000797"
     CDS             7516..8658
                     /locus_tag="VYO63_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1671753.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8667..9881
                     /gene="thiI"
                     /locus_tag="VYO63_000798"
     CDS             8667..9881
                     /gene="thiI"
                     /locus_tag="VYO63_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1671754.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGRAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10016..10839
                     /locus_tag="VYO63_000799"
                     /pseudo
     CDS             10016..10839
                     /locus_tag="VYO63_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            10861..11600
                     /locus_tag="VYO63_000800"
                     /pseudo
     CDS             10861..11600
                     /locus_tag="VYO63_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11613..12779
                     /locus_tag="VYO63_000801"
     CDS             11613..12779
                     /locus_tag="VYO63_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1671755.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     ATTFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            complement(13270..14940)
                     /locus_tag="VYO63_000802"
     CDS             complement(13270..14940)
                     /locus_tag="VYO63_000802"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1671756.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT         4347 a         2959 c         3341 g         4303 t
ORIGIN      
        1 aataaagaga gtacgaaaat tctcatctga aagtatttta gaataattct cttcgtgaat
       61 ttcttcaaaa cagatagctt catcttaggt atgtgatttc tttttgcatt tttgagttag
      121 ataaggtata atggttttat tgtcttttgg ggtcgttacg gattcgacag gcattatgag
      181 gcatattttg cgactcgtgt ggcgacgtaa acgctcagtt aaatataact gcaaaaaata
      241 acacttctta cgctctagct gcctaaaaac cagcaggcgt gacccgattt ggattgctcg
      301 tgttcaatga caggtcttat tattagcgag atacgattaa gccttgtcta gcggtttgat
      361 aagagattga tagactcgca gtttctagac ttgagttatg tgtcgagggg ctgttaaaat
      421 aatacataac ctatggttgt agacaaatat gttggcaggt gtttggacgt gggttcgact
      481 cccaccggct ccattattcc tttgcattcc ttggtaaaac gttgttaaat caacgttttt
      541 tatttttatc tttggtattc ctttgcattc ttttgctaaa aagggagtca caaacagacc
      601 ctattttaaa aaaaggatag aaaaaaggat acaacatttg tcgcatccta aaaataatct
      661 tttttcgacg gaagacatgg gattcgaacc cacgcacgct attacacgcc taccgcgttt
      721 ccaacacggc ctcttaagcc tcttgagtaa tcttccaata cttactcaaa tagtctacca
      781 taaaggctct tatcttgcaa taaaaattct agaaataaga aaaaatgata gattttgaaa
      841 gaaaatgata aaaaatgctt gacttcgaaa gaaagtatga tagaatgaat agtgtaaacg
      901 ataacaggag gtgattcagt gttaaaaaca gaacgtaaac aactaatttt agaggagtta
      961 aatcagcatc atgtagtttc tctagaaaaa ttagttagtt tgctagaaac gtcagaatca
     1021 acggttcgaa gagacttgga tgagttggaa gcggaaaaca agcttcgtcg tgtgcatggt
     1081 ggagcagaac tcccccactc cttacaggaa gaagaaacca ttcaagaaaa atctgtcaaa
     1141 aaccttcaag aaaagaagtt gctggctcag aaagcagcct ctctcattaa agaaaaagat
     1201 gtcatcttta tcgatgctgg aacaacaact gcttttttga ttcatgaatt ggtcaataag
     1261 aatgttacag ttgtgaccaa ctccattcac catgccgctc agttggttga aaagcagatt
     1321 ccaactgtca tggttggagg aaacgtcaag acggcgacag atgctagtat cgggggcgtt
     1381 gctcttaacc agattaacca attgcacttt gaccgtgcct ttatcggaat gaatggtgtt
     1441 gacgatggct attatacgac tcctgatatg gaggagggag ctgtgaaaag agctattttg
     1501 gagaatgcca agcagaccta cgtcttggtg gattcgtcaa aaattggaca aacttgcttt
     1561 gccaaggtag caccactcaa acgcgctatc gttatcacta gtcaagggca tgagctcttg
     1621 caggttatta aggagaaaac ggaggtaata gaagtatgat ttatacagtc acactcaatc
     1681 catccattga ctatatcgtt cgtttggacc aagtcaaagt tggtagtgtc aatcgtatgg
     1741 acagtgatga taagtttgct ggtgggaaag gaatcaatgt cagccgtgtc ttgaaacgtt
     1801 tgaatatacc aaatacagcg acgggattta tcggtggctt tactggtaaa tttatcacag
     1861 atactctagc agaggaagaa atcgagacac gttttgtcca agtggcagaa gatactcgta
     1921 tcaatgttaa aatcaaagca gaccaagaaa cagaaatcaa cggaacgggt ccaactgttg
     1981 aaccggttca gctagaagaa ttgaaagcta ttttatctag tctgacagta gaagatacag
     2041 ttgtctttgc aggttcaagt gctaaaaatc taggcaatgt tatctataag gatttgattt
     2101 ccttgacgcg ccagactggt gcgcaagtgg tctgtgactt tgaaggacag accttaattg
     2161 atagtttgga ctaccagcct cttcttgtaa aaccaaacaa tcatgaactt ggagcgattt
     2221 ttggggttaa actcgaaagt ttagatgaaa ttgagaaata cgctcgtgag ttactggcta
     2281 agggtgctca aaatgttatt atctctatgg ctggtgatgg cgcccttctt gtcacatctg
     2341 agggagctta cttcgctaaa ccaatcaaag gaacagtcaa aaattcagtt ggagctggtg
     2401 attctatggt tgctggattc acaggtgaat ttgtcaaatc aaaagacgca gtagaagcct
     2461 tcaaatgggg agtggcttgc ggaacggcaa ctaccttctc agatgacttg gcaacggcgg
     2521 aatttattaa agaaacatat ggaaaagttg aggtagaaaa aagatgaaaa ttcaagacct
     2581 attgagaaaa gatgtcatgt tgctagattt gcaggcaact gaaaaaacag ctgtcatcga
     2641 cgagatgatt aaaaatttga cagagcacgg ttatgtaaca gattttgaaa catttaaaga
     2701 aggaattttg gcgcgtgaag ctttgacttc tactggtttg ggtgatggaa tcgcaatgcc
     2761 tcacagcaaa aacgctgctg tcaaagaagc gacagttcta tttgctaagt caaacaaggg
     2821 tgttgactac gagagcttgg atggacaagc aactgacctc ttcttcatga ttgcagctcc
     2881 agaaggtgcc aatgatactc acttggcagc cttggcagaa ttgtctcaat acttgatgaa
     2941 agacggtttt gcagacaaac ttcgtcaagc aacatctgca ggccaagtta tcgaactttt
     3001 tgaccaagct tcagaaaaaa ctgaggaact tgttcaagca cctgctaatg actctggtga
     3061 ctttatcgta gctgttacag cttgtacaac aggtattgcc cacacttaca tggcccaaga
     3121 agcccttcaa aaagtagctg ctgaaatggg ggttggtatc aaggtcgaaa ccaacggtgc
     3181 tagcggtgtt ggaaatcaac taactgcaga agatatccgt aaggctaaag ctattatcat
     3241 tgcagcagac aaggccgttg aaatggatcg atttgatgga aaaccattga tcaatcgtcc
     3301 agttgctgac ggtatccgta agacagaaga gctaattaac ttggctcttt caggagatac
     3361 tgaagtctac cgtgccgcta atggtgccaa agctgcaaca gcctctaacg aaaaacaaag
     3421 ccttggtggt gccttgtaca aacacttgat gagtggtgta tctcaaatgt taccattcgt
     3481 tatcggtggt ggtatcatga ttgcccttgc cttcttgatt gacggtgctt tgggtgttcc
     3541 aaatgaaaac cttggcaatc ttggttctta ccatgagtta gcttctatgt ttatgaaaat
     3601 tggtggagct gcctttggtt tgatgcttcc agtctttgcg ggttatgttg cctactctat
     3661 tgctgaaaaa ccgggtttgg tagcaggttt cgtggctggt gctattgcca aagaaggttt
     3721 tgcctttggt aaaattcctt atgccgcagg tggtgaagca acttcaactc ttgcaggtgt
     3781 ctcatctggt ttcctaggtg cccttgttgg tggatttatc gcaggtgcct tggttcttgc
     3841 catcaagaaa tacgttaaag ttcctcgttc actcgaaggt gctaaatcaa tccttctatt
     3901 gccacttctt ggaacaatct tgacaggatt tgttatgcta gctgtgaata tcccaatggc
     3961 tgcaatcaac actgctatga atgacttcct aggcggtctt ggaggaggtt cagctgtcct
     4021 tcttggtatc gtccttggtg gaatgatggc tgttgacatg ggtggaccag ttaataaagc
     4081 agcttatgtc tttggtacag gtacgcttgc agcaactgtt tcttcaggtg gttctgtagc
     4141 catggcagca gttatggctg gaggaatggt gccaccactt gcaatctttg tcgcaactct
     4201 tcttttcaaa gataaattta ctaaggaaga acgtaactct ggtttgacaa acatcatcat
     4261 gggcttgtca tttatcactg agggagcgat tccatttggt gccgctgacc cagctcgtgc
     4321 gattccaagc ttcatccttg gttcagcagt agcaggtgga ctcgttggtc ttactggtat
     4381 caaactcatg gcgccacacg gaggaatctt cgttatcgcc cttacttcaa atgctctcct
     4441 ttacctcgtt tctgtcttgg taggagcaat cgtaagtggt gtggtttatg gttacctacg
     4501 caaaccacaa gcataaaaaa tagaaaaatg aaaagattgg accgtttggt gcagtctttt
     4561 tctcttcccg aaatgcctgt gaaatatggt ataatagaag aatggcaaac aagaatacaa
     4621 gtacaacaag acggagaccg tctaaagcag aactggaaag aaaagaagcg attcaacgaa
     4681 tgttgatttc gttaggaatt gcgattttat tgattttcgc agccttcaaa ttaggggctg
     4741 caggtataac cctttataat ttaattcgct tgctagtggg tagcctagct tatctggcga
     4801 tattcggcct attaatctat ctcttctttt tcaagtggat acgaaaacag gaaggactct
     4861 tatctggctt tttcaccata tttgctggct tactcttgat ttttgaggcc tacttggttt
     4921 ggaaatatgg tttggacaag tccgttctaa aagggaccat ggctcaggtt gtgacagatc
     4981 tgactggttt tcgaacgact agctttgctg gagggggctt gatcggggtc gctctttata
     5041 ttccaacagc ctttctcttt tcaaatatcg gaacttactt tattggttct atcttgattt
     5101 tagtgggttc tctcctagtc agcccttggt ctgtttacga tattgctgaa tttttcagta
     5161 gaggctttgc caaatggtgg gaagggcacg agcgtcgaaa agaggaacgc tttgtcaaac
     5221 aagaagaaaa agctcgccaa aaggctgaga aagaggctag attagaacaa gaagagactg
     5281 aaaaaccctt actcgatttg cctcctgttg atatggaaac gggtgaaatt ctgacagagg
     5341 aagctgttca aaatcttcca cctattccag aagaaaagtg ggtggaacca gaaatcatcc
     5401 tgcctcaagc tgaacttaaa ttccctgaac aggaagatga ctcagatgac gaagatgttc
     5461 aggtcgattt ttcagccaaa gaagcccttg aatacaaact tccaagctta caactctttg
     5521 caccagataa accaaaagat caatctaaag agaagaaaat tgtcagagaa aatatcaaaa
     5581 tcttagaagc aacctttgct agctttggta ttaaggtaac agttgaacgg gccgaaattg
     5641 ggccatcagt gaccaagtat gaagtcaagc cggctgttgg tgtaagggtc aaccgcattt
     5701 ccaatctatc agatgacctc gctctagcct tggctgccaa agatgtccgg attgaagcac
     5761 caatccctgg gaaatcccta atcggaattg aagtgcccaa ctccgatatt gccactgtat
     5821 ctttccgaga actatgggaa caatcgcaaa cgaaagcaga aaatttcttg gaaattcctt
     5881 tagggaaggc tgttaatgga accgcaagag cttttgacct ttctaaaatg ccccacttgc
     5941 tagttgcagg ttcaacgggt tcagggaagt cagtagcagt taacggcatt attgctagca
     6001 ttctcatgaa ggcgagacca gatcaagtta aatttatgat ggtcgatccc aagatggttg
     6061 agttatctgt ttacaatgat attccccacc tcttgattcc agtcgtgacc aatccacgta
     6121 aagccagcaa ggctctgcaa aaggttgtgg atgaaatgga aaaccgttat gaactctttg
     6181 ccaaggtggg agttcggaat attgcaggtt ttaatgccaa ggtagaagag ttcaattccc
     6241 agtctgagta caagcaaatt ccgctaccat tcattgtcgt gattgtggat gagttggctg
     6301 acctcatgat ggtggccagc aaggaagtgg aagatgctat catccgtctt gggcagaagg
     6361 cgcgtgctgc aggtatccac atgattcttg caactcagcg tccatctgtt gatgtcatct
     6421 ctggtttgat taaggccaat gttccatctc gtgtagcatt tgcggtttca tcaggaacag
     6481 actcccgtac gattttggat gaaaatggag cagaaaaact tcttggtcga ggagacatgc
     6541 tctttaaacc gattgatgaa aatcatccag ttcgtctcca aggctccttt atctcggatg
     6601 acgatgttga gcgcattgtg aacttcatca aggctcaggc agatgcagac tacgatgaga
     6661 gttttgatcc aggtgaggtt tctgaaaatg aaggagaatt ttcggatgga gatgctggtg
     6721 gtgatccgct ttttgaagaa gctaagtctt tggttatcga aacacagaaa gccagtgcgt
     6781 ctatgattca gcgtcgttta tcagttggat ttaaccgtgc gacccgtctc atggaagaac
     6841 tggagatagc aggtgtcatc ggtccagctg aaggtaccaa acctcgaaaa gtgttacaac
     6901 aataaaaaaa tagcttcttt ccgagtttgg agggaagcta ttttagtggc tattgattgc
     6961 ttttattttc tgaagttggc gcattggact gtttttcgtt ttcagtagca ggtttacttg
     7021 aagcaggagt agaagagtcc tgagttgctg ttttctgatc ttcttttttc tcttccttga
     7081 cgctagattt tggtgtttcc tcttgctgtg ttttttcttg actagtgtta gtctctttag
     7141 ttggactggt gttttcctta ggggattcct tttggatttc tttgacaatg gttgtcgtct
     7201 ggcttgtcgt aggttctttt ttaatatttt tgttattatc caaggcgttc atgatcgtga
     7261 tacccgcggt aatcaagcca aggatactag cgattgtagc aacggttttt tgaaagactg
     7321 taaagccttt tttgatgtgt tctgtttttg gttttgaagt tctacgatag ttagacatac
     7381 attctctcct ttgattatga tttattatac tctgtttctt taaaaaaatc ttaaaaaaag
     7441 agaaattgcc ctttcttgtc gcaggctccg tttcatgata caatagaagg agtgatttta
     7501 gaaagcgtga gaaacatgat ttactttgat aattcggcga cgaccaggcc ctatccagaa
     7561 gcccttgaaa cctatatgca ggtggcttca aaaattttag gaaatccatc tagtctccat
     7621 cgtttgggag accaggcaac acgaatttta gatgcttccc gtcaacagat tgcagattta
     7681 atcggtaaga aaagtgatga aatcttcttt acatctggtg ggacagaagg ggataactgg
     7741 atcatcaagg gagtagcatt tgaaaaagcc cagtttggca agcacatcat cgtatcagcc
     7801 attgaacatc cagcagttaa ggaatcagcc ctctggttga aagctcaagg ttttgaagtg
     7861 gattttgctc cagttgataa caaaggattt gtggatgttg aggcgctaga agatttgata
     7921 agacctgata cgaccctcgt ttccatcatg gcagttaaca atgaaattgg ctctgttcag
     7981 cccattgagg ctatttcaaa actattggca gacaagccaa ctatttcctt ccacgttgat
     8041 gcggttcagg cgcttgccaa gattccgact gaaaagtatc tgacagaacg agtggatttc
     8101 gcgactttct ctggtcacaa gttccacgga atccgtggtg ttggttttat ctatatcaag
     8161 tctggcaaga agattacgcc tcttttaaca ggtggtggtc aggaacgtga ttaccgttcg
     8221 acaactgaaa atgtggcagg aattgcagcg accgctaagg ctctccgttt atctatggaa
     8281 aagctagata tctttaggag caagactggg cagatgaagg cagtgattcg tcaagcactt
     8341 ctggactatc cggatatatt tgtcttttca gatgaggaag acttcgcccc tcatattctg
     8401 acttttggaa tcaagggtgt tcgtggtgag gtcattgttc acgcctttga agactatgat
     8461 attttcatct caacgacctc ggcttgttcg tccaaggctg ggaaaccagc cggaaccctg
     8521 attgctatgg gagtggacaa ggataaggct cagtcagctg tgcgtcttag tctagacctt
     8581 gaaaatgata tgagtcaggt cgagcagttc ttgaccaagc taaaattaat ttacaatcaa
     8641 actagaaaag taagatagga gcattcatgc agtattcaga aattatgatt cgctacggtg
     8701 agctgtcaac caagggcaaa aatcgtatgc gtttcatcaa taaacttcgc aataatattt
     8761 cagacgtttt gtctatctat ccccaagtta aggtaacagc agatcgcgac cgtgcccacg
     8821 cttacctcaa tggagcagat tacacagcag tagcagaatc gctcaaacaa gtatttggaa
     8881 ttcaaaactt ttcccctgtt tataaggttg aaaaatctgt agaagttctg aagtctgctg
     8941 tccaagagat tatgcaggac atctacaagg aaggcatgac ctttaaaatc tctagcaagc
     9001 gtagcgacca cacctttgaa ttggacagtc gtgaactcaa ccaaacgctt ggaagggctg
     9061 tattcgaagc cattccaaac gtacaagctc aaatgaaaag tcctgacatc aatcttcagg
     9121 tggagatacg tgaagaagca gcctatcttt cttatgaaac cgttcgaggg gcaggtggat
     9181 taccagtggg aacttctggt aagggcatgc tcatgttgtc agggggaatc gattcccctg
     9241 tagctggcta tctagcgctt aaacgagggg tagatataga ggcagttcac tttgccagcc
     9301 caccttacac gagtcctggt gctcttaaaa aagcccaaga tttgacccgt aaattaacca
     9361 aatttggggg aaatatccag tttatcgaag ttccttttac agagattcaa gaggaaatca
     9421 aagccaaagc gccagaagcc taccttatga ccttgacgcg tcgttttatg atgcgtatta
     9481 ctgaccgtat tcgtgaggta cgaaatggtt tagttatcat caatggggaa agtctaggtc
     9541 aagtagccag ccaaaccctt gaaagtatga aggctatcaa tgctgttacc aacactccca
     9601 tcattcgtcc tgtggttacc atggacaagt tggaaatcat tgacatcgcc caggaaatcg
     9661 atacctttga catttcaatc caaccgtttg aagactgttg taccattttt gcaccagatc
     9721 gtccaaaaac aaatcctaaa attaagaatg cggagcagta cgaagcgcgt atggatgttg
     9781 aaggcttggt tgagcgagca gtggctggaa tcatgattac tgaaatcaca cctcaagccg
     9841 aaaaagatga agttgatgac ttgattgaca atctgctcta attcagaaaa tccaaaagaa
     9901 tagcgaaaat cagtaaaaaa agttagtttt ttctctaaaa acaggtaaaa aactaacttt
     9961 ttttattttt atgatataat gatataaaat tttgaatata gagagttttc tggcaatgaa
    10021 tcaatcctac ttttatctaa aaatgaaaga acacaaactc aaggttcctt atacaggtaa
    10081 ggagcgccgt gtacgtattc ttcttcctaa agattatgag aaagatacag accgttccta
    10141 tcctgttgta tactttcatg acgggcaaaa tgtttttaat agcaaagagt ctttcattgg
    10201 acattcatgg aagattatcc cagctatcaa acgaaatccg gatatcagtc gcatgattgt
    10261 cgttgctatt gacaatgatg gtatggggcg gatgaatgag tatgcggctt ggaagttcca
    10321 agaatctcct atcccagagc agcagtttgg tggtaagggt gtggagtatg ctgagtttgt
    10381 catggaggtg gtcaagcctt ttatcgatga gacctatcgt acaaaagcag actgccagca
    10441 tacggctatg attggttcct cactaggagg caatataccc agtttatcgg tttggaatac
    10501 caagaccaaa ttggttgctt gggcgttttt tcatctgcaa actggctcca ccaagaagcc
    10561 tttaaccgct atatcgagtg ccagaaacta tcgcctgacc agcgcatctt catctatgta
    10621 ggaacagaag aagcagatga tacagacaag accttgatgg atggcaatat caaacaagcc
    10681 tatatcgact cgtcgctttg ctattaccat gatttgatag cagggggagt acatctggat
    10741 aatcttgtgc taaaagttca gtctggtgcc atccatagtg aaatcccttg gtcagaaaat
    10801 ctaccagatt gtctgagatt ttttgcagaa aaatggtaag ttaagaaagg aaaaaacgaa
    10861 atgcatattg aacatcttag ccactggagt ggtcatctta accgtgaaat gtaccttaac
    10921 cgttatggac atggtgggat tccagttgtg gtctttgctt catcaggtgg tagtcacaac
    10981 gaatactatg attttggcat gattgatgcc tgtgcttcct ttatcgagga aggccttgtc
    11041 cagttcttta ccctatctag tttggatagt gagagctggt tggttacttg gaaaaatgct
    11101 catgaccaag cggaaatgca ccgtgcctac gaatgttatg tgattgagga ggccattctt
    11161 ttatcaagca caagacaggt tggtttgatg gcatgatgac gacaggttgc tctatgggag
    11221 cctatcatgc actcaatttc ttcctccagc atccagatgt ctttaccaaa gtgattgctc
    11281 tcagtggtgt ttacgacgca cgtttctttg tcggtgatta ctacaacgat gatgctattt
    11341 accaaaactc gccagtagat tatatttgga accaaaacga cggctggttt attgaccgtt
    11401 accgtcaggc agagattgtg ctgtgtacgg ggcttggagc ctgggaacaa gatggtttgc
    11461 catcctttta caagctcaaa gaagcctttg acaagaaaca aattccagcc tggtttgctg
    11521 aatggggaca tgatgtcgcc catgactggg aatggtggcg taaacaaatg ccttatttcc
    11581 tcggtaatct ctatttataa aaggagttac ctatgaatta ccttgttatt tctccctact
    11641 atccacaaaa ctttcaacag tttaccatcg aactagctaa taaaggcatc acagtcttgg
    11701 gaattggtca agagtcttac gagcaattgg atgagccctt gcgcaatagc ttgaccgagt
    11761 attttcgtgt tgataatctt gagaacatag atgaagtcaa acgtgcagtt gcttttctct
    11821 tttataaaca tggtccaatt gaccgcatcg agtctcacaa tgaatactgg cttgagctag
    11881 acgcaacact cagagaacaa ttcaatgttt ttggtgccaa accagaggat ctcaaaaaga
    11941 cgaaatataa gtctgaaatg aagaaacttt tcaaaaaagc aggtgttcct gtggtacctg
    12001 gagctgttat caagacggaa gcagatgttg atcaagcagt gaaagaaatc ggtcttccaa
    12061 tgattgccaa acctgataat ggagtgggag cagccacaac ctttaaactt gagacagaag
    12121 acgatatcaa tcacttcaag caagaatggg accattcaac cctttatttc tttgaaaaat
    12181 ttgtcacttc cagcgaaatc tgtacctttg acgggctcgt ggacaaggat ggaaagattg
    12241 tcttctcaac aacctttgac tacgcctata caccgcttga cctcatgatt tataagatgg
    12301 acaattctta ttatgtgctc aaggatatgg atcctaaact gcgcaagtat ggggaagcaa
    12361 ttgtcaaaga atttggtatg aaagaacggt ttttccatat tgagttcttc cgtgaggggg
    12421 acgattatat taccatcgag tacaataacc gccctgcagg tggttttacc attgatgttt
    12481 ataactttgc tcattccttg gacctctatc gtggctatgc agctattgtc gcaggagagg
    12541 agttcccggc gtcagacttt gaaactcagt attgtttggc tacttctcgc cgtgcaaatg
    12601 ctcactatgt ttattcagaa gaggatttgc ttgccaaata tagccagcag ttcaaagtta
    12661 aaaaagtcat gccagctgcc ttcgcggaac ttcaaggaga ttacctgtat atgctgacca
    12721 ctccgagtcg acaagaaatg gagcagatga ttgcagattt cggacaacgt caagaataag
    12781 aactatcgga ttaaggaaat taactccctt aatccttttg ttttgtctga taaaaaataa
    12841 gagcatccca acaaggtagc tatcataaaa cttgttcgat aactatttga agcaggatta
    12901 ggtggtcaga aattgaattt taatatttca attgagtcat agtattgtgt ttgcgtatcc
    12961 ttaaatcagc taaaaggatc catgacgaca cctatacgat atagttttca agataccaaa
    13021 caagtctatt aatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggtttc
    13081 tcaaaacact gttttgaggt tgtggataga actgacagag tcagtatcat atacctacgg
    13141 caaggtgaag ctgacgtagt ttgaagagat tttcgaagag tataaatctt attgatgaac
    13201 tgcttgcagt ctgagaaaaa atgagcttgg atattatttc caaactcact taaagtcaat
    13261 ttcaatccac tagaacaagc ctagtacagt tccatcgctt tcaacatcca tgttgagagc
    13321 tgctggacgt tttggaagac ctggcatggt cataacatca ccagttaagg caacgatgaa
    13381 gcctgcacct aattttggta ccaattcacg aatggtaatt tcaaagtttt ctggtgctcc
    13441 aagcgcattt ggattgtctg agaaactgta ttgagtttta gccatacaga ttggcaattt
    13501 gtcccaaccg ttttgaacga tttgagcaat ttgtgtttga gatttcttct caaagttcac
    13561 tttgctacca cgatagattt cagtgacaat tttttcaatc ttttcttgga cagaaaggtc
    13621 attatcgtac aaacgtttat agttagctgg attttcagca attgtcttaa caactgtttc
    13681 ggcaagtgct actccacctt ctgctccatc agcccagaca ctagccaatt caactggtac
    13741 atcgattgag gcacagagtt cttttaagac tgcaatttca gcttctgtat cagatacaaa
    13801 ttcgttaata gctacaactg ctggaatacc gaacttacgg atattttcaa cgtggcgttt
    13861 caagttagca aaacctgcac gaactgcctc tacattttct tcagtcagag cgtctttagc
    13921 cacaccacca ttcatcttaa gggcacgaag ggttgcgaca ataacaactg catctggaga
    13981 tgttggcaag tttggtgtct tgatatcaag gaatttctca gcaccaaggt ccgcaccaaa
    14041 accagcttca gtaacagtgt aatcagccaa gtgaagggct gttgtcgtcg ccaaaacaga
    14101 gttacagcca tgagcgatat tggcaaatgg accaccgtgt acaaaggcag gtgtaccgta
    14161 aattgtctga accaagtttg gcttaatagc atccttcaaa atcaaagcca aggcaccctc
    14221 aacctgcaaa tcacctacag aaacaggcgt acggtcatag cgataaccaa taacgatatt
    14281 cgccaaacga cgtttcaagt cctcgatgtc cgttgccaag caaagaattg ccatgatttc
    14341 tgaagcaact gtaatatcaa aaccatcctc acgtggaata ccgtttagag gaccaccaag
    14401 accaacagtc acatggcgga gcgcacggtc gttcaagtcc acaacgcgtt tccagaggat
    14461 acgacgttga tcaattccca gctcattccc ttggtgcaag tggttgtcaa tcaaggcaga
    14521 aagggcattg ttggcagttg taatagcatg catatctcca gtaaagtgga ggttgatgtc
    14581 ttccattggc agaacttgtg catacccacc accagcagca ccacccttga tccccatgac
    14641 tggaccaaga gacggttcgc ggatagcaat catggttttc ttgccaatct tgttcaaggc
    14701 atccgcaaga ccaatggtaa gcgtcgactt tccttcacct gcaggtgttg ggttgatggc
    14761 agtaaccaag atcaatttac cgactggatt gctctcaact gcccgaattt tatcaaagct
    14821 gagtttagcc ttgtactttc cgtacaactc caaatcgtcg taagaaatac caagtttctc
    14881 tacaacatca acaattggct tcaactcaat actctgtgcg atttcaatat ctgttttcat
    14941 tcaaaattcc
//
LOCUS       DAVLYR010000032        14950 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLYR010000032 DAVLYR010000000
VERSION     DAVLYR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14950
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.032"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            400..1131
                     /gene="budA"
                     /locus_tag="VYO63_000803"
     CDS             400..1131
                     /gene="budA"
                     /locus_tag="VYO63_000803"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1671757.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            1141..1347
                     /locus_tag="VYO63_000804"
     CDS             1141..1347
                     /locus_tag="VYO63_000804"
                     /inference="COORDINATES: protein motif:HMM:NF021592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1671758.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLDLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALL"
     gene            1384..1893
                     /locus_tag="VYO63_000805"
     CDS             1384..1893
                     /locus_tag="VYO63_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1671759.1"
                     /translation="MVAFFEETARLVFFKWLEKKRSLEKADALAYGLGHGGLELIFLG
                     LTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQSLSVWQIYLLGFERILALGFQLLL
                     TVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQVGWLTNPVLVEVILALELVLVAYG
                     TKEIFCKKS"
     gene            2005..2910
                     /gene="murB"
                     /locus_tag="VYO63_000806"
     CDS             2005..2910
                     /gene="murB"
                     /locus_tag="VYO63_000806"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1671760.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            3071..4228
                     /locus_tag="VYO63_000807"
     CDS             3071..4228
                     /locus_tag="VYO63_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671761.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            4209..5015
                     /locus_tag="VYO63_000808"
     CDS             4209..5015
                     /locus_tag="VYO63_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671762.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            5012..5785
                     /locus_tag="VYO63_000809"
     CDS             5012..5785
                     /locus_tag="VYO63_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671763.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            5782..6852
                     /locus_tag="VYO63_000810"
     CDS             5782..6852
                     /locus_tag="VYO63_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1671764.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGSEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            7121..7360
                     /locus_tag="VYO63_000811"
     CDS             7121..7360
                     /locus_tag="VYO63_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671765.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLNDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            7635..8120
                     /locus_tag="VYO63_000812"
     CDS             7635..8120
                     /locus_tag="VYO63_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1671766.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            8142..10760
                     /gene="alaS"
                     /locus_tag="VYO63_000813"
     CDS             8142..10760
                     /gene="alaS"
                     /locus_tag="VYO63_000813"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1671767.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(10850..12304)
                     /locus_tag="VYO63_000814"
     CDS             complement(10850..12304)
                     /locus_tag="VYO63_000814"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1671768.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            12483..13232
                     /locus_tag="VYO63_000815"
     CDS             12483..13232
                     /locus_tag="VYO63_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671769.1"
                     /translation="MIEFQNVSKLYGDKKALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            13213..14847
                     /locus_tag="VYO63_000816"
     CDS             13213..14847
                     /locus_tag="VYO63_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671770.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSLMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVDPFSTEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
BASE COUNT         4118 a         2976 c         3333 g         4523 t
ORIGIN      
        1 ttatcctttg gaaaatcctt ttgtttgcat ttatttttgc aattattaat acaatattta
       61 tgtttactag gaacaaaaaa taaataatga tttagttaaa taaaaagcga cccctaggga
      121 agtgtgttcc ttaggggttg ttgtgctagt gcaagttttg tttctgtcaa aatctcaggc
      181 tacccgtcgg aaatcgtcca gactcgatac catttttcag ataggaaacg acctctttgt
      241 acttgctttg tttcttcatt ccagacctcc cttgattttg ttacattgta ccctgttttt
      301 ccttcttggc aatgtctaga gcgtttctct cgttcacatc taggggcaaa tgtggtatac
      361 ttaggagtaa gatttataga ataacagaca agaaagtgaa tggataagaa cgtgcaggaa
      421 ccagtgaaat tatttcaata caatactctt ggagccttga tggctggcct ttatggtggt
      481 actatgacag taggagaatt gctagaacat ggtgaccttg gtttgggaac cttggattcc
      541 attgatgggg aattgattgt cttggatggc aaggcttatc aggccaaagg gtcaggagac
      601 cagccagaaa ttgtggaagt gtcaccagat gcccttattc cttacgctgc agtggtaccg
      661 catcaggcag aggtcatttt ccgccagcgc tttgagatga cagacaagga attgaaagaa
      721 cgaattgagt cttattatga tggggaaaat cttttccgct ctatcaagat tcggggggaa
      781 ttttcacata tgcatgtgcg tatgattcct aagtcaacac ccgataccaa gtttgctgat
      841 gtcgcaaccc atcaaccgga atatagtcgt gacaatgttg cggggactat tgttggtttc
      901 tggacgcctg agattttcca tggggtcagt gtggcaggct accatctgca cttcatatca
      961 gatgatttga ccttcggtgg acatgtcatg gattttgtca tcaaggaagg cattatcgag
     1021 gtgggagcag ttgaccagtt ggaccaacgt ttcccagtcc aagaccgtca atacttgttt
     1081 gctaagttca atgttgacga gatgaaaaaa gatattgaaa aggcagaata ggagaagaaa
     1141 atgaccattc atatcattat taccatgttg ctgttgctag cttttctgat aggaagcatt
     1201 tggtttgcca aaaagaaata ccagattaat ctagctgtct tggacttggg tgcagtcgct
     1261 ttctttgtct cttcgcagat tttggaaaaa ctggtgcata tcttgatttt gcatcctcaa
     1321 aaagacggta gtattgccct cttgtaagtt catccgctta tctatattat ctatggccta
     1381 gccatggtag cattttttga ggaaacggct cgtcttgttt tcttcaaatg gttggagaaa
     1441 aagagaagtt tggaaaaggc agacgccttg gcctatggat tggggcatgg tggcttggag
     1501 ttgatttttc taggccttac tagtttactc aatctttaca ttgttctttc ggcagttcaa
     1561 actcagaatc cacaggttat gcaattactg tctgaaaata tgttaaaaac cattcagtca
     1621 ctttcggtct ggcagattta tttgcttggt tttgagcgaa tcttggcgct tggtttccaa
     1681 ttacttttga cagtttgggt ttatcaagct gttcgccaga agaaatggat ttatctccta
     1741 acagcctatg gcttgcatgc cttctttgat ctggcaccat ctcttttcca agtaggctgg
     1801 ttgacaaatc cagtcttggt tgaagtgatt ctagcactgg agctcgttct ggtcgcctat
     1861 ggaaccaagg aaatcttttg taaaaaatca taaaaagagg ggaacctctt tttcttatgc
     1921 aaaatccaaa caaggtattt ttatggtcgt caaatgtctc taaaaatggt ataatggaat
     1981 gaattttgta aaaggaagag tgacatgtct gtaagagaaa aaatgcttga aatcttagaa
     2041 ggaattgata tccgttttca ggaacccttg catagctata gttatacaaa agtaggtgga
     2101 gaggctgatt atttggtctt tccacgaaat cgttttgagt tggctcgcct tgtgaaattt
     2161 gccaaccaag aaaatatccc ttggatggtt cttggcaatg caagcaatat catcgttcgt
     2221 gatggtggga ttcgtggatt tgtcatcttg tgtgacaagc tcaataacgt ttctgttgat
     2281 ggctatacca ttgaagcaga agctggggct aacttgattg aaacaactcg cattgccctc
     2341 cgtcatagtt tgactggctt tgagtttgct tgtggtattc caggaagcgt tggcggtgct
     2401 gtctttatga atgcgggtgc ctatggtggc gagattgctc acatattgca gtcttgtaag
     2461 gtcttgacca aggatggaga aatcgaaacc ctgtctgcta aagacttggc ttttggttac
     2521 cgccattcag ctattcagga gtctggtgca gttgtcttgt cagttaaatt tgccctagcc
     2581 ccaggaaccc atcaggttat caagcaggaa atggaccgct tgacgcacct acgtgaactc
     2641 aagcaacctt tggaataccc atcttgtggc tcggtcttta agcgtccagt cgggcatttt
     2701 gcaggtcagt taatttcaga agctggcttg aaaggctatc gtatcggtgg cgtagaagtg
     2761 tcagaaaagc atgcaggatt tatgatcaat gtcgcagatg gaacggccaa agactacgag
     2821 gacttgatcc aatcggttat cgaaaaagtc aaggaacact caggtattac gcttgaaaga
     2881 gaagtccgga tcttgggtga aagcaagtag gaagctgtat ttgaaaagct gctgctatgt
     2941 catggaaagg ggggtgaaag cctatcggta gcgaagatgt atgcaggtgg ttttactccc
     3001 tgcaagaggt agtggggacc tgacagagcc ccgatcggtt aatctatgaa aaagaaggaa
     3061 tttatgccaa ttgaaaaaac caattatcga attcaaaaac gtctctaaag tttttgaaga
     3121 cagcaacacc aaggttctca aagacatcaa ctttgagttg gaagaaggga aattctacac
     3181 ccttctaggt gcatctggtt cggggaaatc aactatccta aacattattg caggtttact
     3241 ggatgcgacg acaggagata tcatgctaga cggtgttcgt atcaatgata ttccaaccaa
     3301 caagcgcgac gtacatactg tcttccaatc ctatgccttg ttcccacata tgaatgtgtt
     3361 tgaaaatgtt gcctttccac ttcgcttgcg caagattgac aagaaagaaa tcgagcaacg
     3421 cgtagcggaa gttctcaaga tggttcagtt ggaaggttat gaaaaacgtt ccatccgcaa
     3481 actttctgga ggacaacgtc agcgtgtggc catcgcccgt gctatcatca accaaccccg
     3541 tgtggtcttg ttggacgagc ctttatcagc gctggacttg aaattgagaa cagacatgca
     3601 gtacgaattg cgtgaattac aacaacgatt gggcattacc tttgtctttg tcactcacga
     3661 tcaggaagaa gctcttgcca tgagtgactg gattttcgtt atgaatgatg gcgagattgt
     3721 ccagtctgga acccctgtgg acatctacga tgagccaatc aaccactttg ttgccacctt
     3781 tatcggggag tcaaacatct tgccaggtac catgattgag gactacttgg tcgaatttaa
     3841 cggcaaacgc tttgaagcgg ttgatggtgg gatgaagcca aatgaacctg ttgaggtcgt
     3901 tattcgtcca gaggacttgc gcattaccct tcctgaagaa ggcaaactcc aagttaaggt
     3961 cgacacccag ctcttccgtg gagttcatta tgaaattatc gcctatgacg aacttggaaa
     4021 tgaatggatg atccactcaa cccgtaaggc catcgtgggt gaggaaatcg gtctggactt
     4081 tgaatcagaa gacatccaca tcatgcgtct caatgaaacc gaagaagagt tcgatgctcg
     4141 tatcgaagaa tacgtggaaa tcgaagagca agaagcaggt ctgatcaatg cgatcgagga
     4201 ggaaagagat gaagaaaaca agctctaaac tctttgtagt gccctacatg ctttggattg
     4261 ccctctttgt attggcaccc ttggtcttga ttttcggaca atcctttttc aacattgaag
     4321 gccagtttag tttagaaaat tataaatctt actttgcgtc acaaaacttg acctacctca
     4381 aaatgagttt caactcagtg ctttatgcag ggattgtgac ctttgtgacc ttgcttatca
     4441 gctatccaac agccctcttt ttgacccgtc tcaagcaccg tcaactctgg ctcatgctga
     4501 ttatccttcc tacctggatc aatttgctcc ttaaagccta tgcttttatc gggatttttg
     4561 gtcaaaatgg ctctattaac caattcttgg aatttatcgg aattggttca caacagttgc
     4621 tttttaccga tttttccttt atctttgtcg caagctacat cgagctcccc tttatgattt
     4681 tgccgatttt caatgtctta gacgatatgg ataataatct catcaatgct agttatgacc
     4741 ttggtgcaac taagtgggag accttccgtc atgtcatctt ccctctatct atgaacggtg
     4801 tgcgaagtgg ggttcagtcg gtctttatcc caagtttgag tctcttcatg ctgacccgtt
     4861 tgattggtgg gaaccgcgtt atcaccttgg ggacggctat tgagcagaat tttctaacca
     4921 atgacaacta tggtatgggt tcaaccatcg gtgtgattct catcctgacc atgttcatca
     4981 ccatgtgggt gactaaggaa aggagagaac gatgaaaaaa tttgccaacc tttatctggg
     5041 actggtcttt ctggtcctct acctgcctat cttttacttg attggctatg cctttaatgc
     5101 tggtgatgat atgaatagct ttacaggttt tagctggact cactttgaaa ccatgtttgg
     5161 agatgggaga ctcatgctga ttttggctca gacatttttc ttggccttcc tatcagcctt
     5221 gatagcgacc attatcggga cttttggtgc catttacatc taccagtctc gtaagaaata
     5281 ccaagaagcc tttctatcac tcaataatat cctcatggtt gcgcctgacg ttatgattgg
     5341 tgctagcttc ttgattctct ttacccaact caagttttca cttggctttt tgaccgttct
     5401 atctagtcac gtggccttct ccattcctat cgtggtcttg atggtcttgc ctcgactcaa
     5461 ggaaatgaat ggcgacatga ttcatgcggc ctatgacttg ggagctagtc aatttcagat
     5521 gttcaaggaa atcatgcttc cttacctgac tccgtctatc attactggtt atttcatggc
     5581 cttcacctat tcgttagatg actttgccgt gaccttcttt gtaacaggaa atggcttttc
     5641 aaccctatca gtcgagattt actctcgtgc tcgcaagggg atttccttag aaatcaatgc
     5701 cctgtctgct ctagtctttc tctttagtat tatcctagtt gtaggttatt actttatctc
     5761 tcgtgagaag gaggagcaag catgaaaaaa atctattcat ttttagcagg aattgcagcg
     5821 attatccttg tcttgtgggg aattgcgact catttagata gtaaaatcaa tagtcgagat
     5881 agtcaaaaat tggttatcta taactgggga gactatatcg atcctgaact cttgactcag
     5941 tttacagaag aaacaggaat tcaagttcag tacgagactt ttgactccaa cgaagccatg
     6001 tacactaaga taaagcaggg tggaacgacc tacgatattg ccattccaag tgaatacatg
     6061 attaacaaga tgaaggacga agacctcttg gttccgcttg attattcaaa aattgaagga
     6121 atcgaaaata tcggatcaga gtttctcaac cagtcctttg acccaggtaa taaattctcc
     6181 atcccttact tctggggaac cttaggaatt gtctacaacg aaaccatggt agatgaagcg
     6241 cctgagcatt gggatgacct ttggaagccg gagtataaga attctatcat gctctttgat
     6301 ggggcgcgtg aggtgctggg actaggactc aattccctcg gctacagcct caactccaag
     6361 gatctgcagc agttggaaga gacagtggat aagctctaca aactgactcc aaatatcaag
     6421 gctatcgttg cggacgagat gaagggctat atgattcaga ataatgttgc aatcggcgtg
     6481 accttctctg gtgaagccag ccaaatgtta gaaaaaaatg aaaatctacg ttatgtggta
     6541 ccgacagagg ccagcaatct ttggtttgac aatatggtca ttcccaaaac agttaaaaac
     6601 caaaactcag cctatgcctt tatcaacttt atgttgaaac ctgaaaatgc tctccaaaat
     6661 gcggagtatg tcggctattc aacaccaaac ctaccagcga aggaattgct cccagaggaa
     6721 acaaaggaag ataaggcctt ctatcccgat gttgaaacca tgaaacacct agaagtttat
     6781 gagaaatttg accataaatg gacagggaaa tatagcgacc tcttcctaca gtttaaaatg
     6841 tatcggaagt aggagataaa cgtaagaaga cgaatcagtc aagctggttc gtttttaagg
     6901 aatagaagtc agtttttaga gccctatctt tttctattct agttgtttat ttgcttattt
     6961 gcttatttgc ttatttgctt gcctagtagt atactaagcc tatactcttc aaaccatgtc
     7021 agcgttgcct tgccgtactc aagtacagct tgaggctagc ttcctagttt gctttttgat
     7081 ttttattgag tattaacctt acagaaagcg gcaacaaata atgaaaatgt ctactttttt
     7141 caaaaaaagt ttctggccaa cctttatgat tgttaaccaa actgctatac tattccattt
     7201 aaatgatggc ttggatcgac aatacctaac aactgagtcc atatattggg tgataggcac
     7261 ctttatattt ggaaatattc tagtagctgt gtttagtaat atgaaaattt gggataaaaa
     7321 gaaaaatggc agtaagaaaa aatatatcct gaaaaagtaa tcagagatgt agggaattgg
     7381 tctaattcaa cggagatcat ttccagttga cgcaagctta aaaaaacgtt ataataagaa
     7441 agttgagaat tgattttcac ttgtcttagt ccagagaaat ggcggtgctg cgagccatct
     7501 aagctaggaa gtcatgctac tcaatcatac aattgcataa gaacaagaga atgacaagtt
     7561 cattgaatga aggtggtacc gcggtttttc gcccttcgtg atatgagctt gtcttttgat
     7621 ttttggaggt gttgatgaaa acatttcttg tcaaacaaaa gtttcgtctt ggaggcgaac
     7681 gcttcgctat caaggatgac aggggagaaa ttgcctatca ggtggaggga tcatttttta
     7741 agattcccaa aacttttacc atctatgatg cggctggtga acaagtcagt cagatcagta
     7801 aagaaatctt gaccttgctt cctcgttttg agattcagct tcgggatggc tcgagttttg
     7861 tcattcgtaa gaagttgacc ttctggcgag ataagtatga gtttgataat ctaggtcttc
     7921 gtatcgaggg caatatctgg gatttgaatt tcaaattgct ggatgatcgc gatcagttga
     7981 ttgcggaaat taagaaggaa ctcttccatc tgacctctac ctataccgta acggttcttg
     8041 aagacgctta tgcagaccta gtcatttccc tctgtgtcgc gattgactat gtggaaatgc
     8101 tggaaagcca atcacattaa acaagtgaat aaggagacaa tatgaaacaa ctatctagtg
     8161 cacaagtacg ccaaatgtgg cttgatttct gggcgaccaa aggtcactca gtagaaccat
     8221 cagtaagttt ggttcctgta aatgacccaa ctcttttgtg gatcaactct ggggtagcaa
     8281 cccttaagaa atactttgac gggaccatta tccctgaaaa tccacgtatt accaatgccc
     8341 aaaaggctat ccgtaccaac gacatcgaaa acgtagggaa gactgcgcgt caccatacca
     8401 tgtttgaaat gctggggaac ttctctatcg gggattactt ccgtgacgaa gctatcactt
     8461 gggcttatga gcttttgaca agccctgaat ggtttgattt ccctgctgaa aaactttaca
     8521 tgacctacta tccagacgat aaagattctt acaaccgctg gattgaagtg ggagtggatc
     8581 caagtcactt gattccaatt gaggacaact tctgggaaat cggtgcggga ccttctggac
     8641 cagatacaga aatcttcttt gaccgtgggg aagcctttga cccagaaaat atcggtcttc
     8701 gcctgcttgc agaagatatt gaaaacgacc gttatattga aatctggaac atcgttttgt
     8761 cacaatttaa cgcagaccct gctgttcctc gtagcgaata caaggaattg ccacataaga
     8821 acattgatac gggcgctggt ttggagcgtt tggtggccgt tatccaaggg gctaagacca
     8881 actttgaaac ggacctcttc atgccgatta tccgtgaagt cgagaaattg tctggtaagg
     8941 tttatgacca agatggcgac aacatgagct tcaaggttat cgcagaccac attcgttctc
     9001 tttcatttgc tatcggtgat ggtgcccttc caggaaatga aggtcgtggt tatgttcttc
     9061 gtcgtcttct ccgtcgtgct tctatgcatg gtcaaaaatt gggtatcaac gagcctttcc
     9121 tttacaaatt ggttccaact gttggaaaaa tcatggaaag ctactaccca gaagtgcttg
     9181 agaaacgtga ctttattgag aaaatcgtta agagcgaaga agaatcattt gcccgtaccc
     9241 ttcactcagg tcaacacttt gcccaaggca ttgtggcaga cttgaaagaa aaaggtcaat
     9301 ctgtcattgc tggtcaagat gtctttaaac tctatgatac ttatgggttc ccagttgaat
     9361 tgactgaaga aatcgctgaa gaagctggta tgactgtaga ccgtgaaggt tttgaagcag
     9421 ccatgaaaga acagcaagaa cgcgcgcgtg cgtcagctgt caagggtggc tcaatgggta
     9481 tgcaaaatga aactcttcaa aacatcactg tagaaagtgt cttcaactac aatactagcc
     9541 aattgtcttc taaattggta gctatcgttg ctgacaatgc agaagtagga gctgtttcag
     9601 aaggaactgc ctctcttatc tttgcggaaa cgtcatttta tgctgaaatg ggtggacagg
     9661 tagctgacta cggacaaatc ttggatgagt caggtaagat tgtggctact gtgaccaatg
     9721 ttcagaaagc cccaaatggt caagcccttc atacagttga agtccttgca ccgcttgcct
     9781 tgaaccaaga atataccttg gcaattgata gcaatcgccg tcaccgtgtc atgaaaaacc
     9841 acactgcgac tcatttgctt cacgctgccc ttcacaatat ccttggaaac cacgcaacac
     9901 aggcaggatc tcttaacgaa gttgaattcc ttcgctttga ctttacccac ttccaagcag
     9961 taactgctga ggaacttcgt gcgattgaac agcaagttaa cgagaaaatt tgggaagcac
    10021 ttgaagtgaa gacagttgaa acggatattg acactgctaa agaaatggga gctatggccc
    10081 tctttggtga gaaatacggc aaggaagttc gtgtcgtgac tatcggtgac tactctattg
    10141 aactttgtgg tggtactcat gttggcaaca cttctgagat tggtctcttc aaaattgtca
    10201 aagaagaagg aatcggttca ggaactcgcc gtatcttggc agtgactggt aaggaagcct
    10261 ttgaagccta tcgtgaacaa gaggatgctc ttaaagctgt cgcagcaacc ttgaaagcac
    10321 ctcaagtcaa ggaagtacct cacaaggtag aaggacttca agaacaactt cgtcaacttc
    10381 aaaaagaaaa tgctgagttg aaagaaaaag ccgcagctgc agccgcaggc gatatcttca
    10441 aagatgttaa ggaagtcaac ggtcatcgtt acattgctag tcaagtgtct gtatccgatg
    10501 caggtgccct tcgtaccttt gcagataact ggaaacaaaa agactactct gatcttcttg
    10561 tcctagttgc cgctatcggt gacaaagtca atgtccttgt agcaagcaag acaaaagacc
    10621 ttcatgcagg aaaccttgtc aaagaattag caccaatcat cgatggacgt ggtggtggta
    10681 aaccagacat ggccatggca ggaggaagca accaacctaa gatccaagaa ttgttggatg
    10741 cagtagcagg taaattgtaa tcaagtaaag atctatccat ttgggtaggt ctttttgtga
    10801 atacaaaaaa gccaaatccg cttggaccta gcttggttat tatgagctat tagatggtat
    10861 tgactgccca gacacttacg gatctagctg agacagggaa ttgtccataa ccttcctcat
    10921 caattgtaac ttgaccttgg tggttaccaa gtaaatctac aaaggtttga ttagtccatt
    10981 cttgaccgac aaacattgac ttgctgtttt cttggtcatt tgagataagg actgcgattg
    11041 gggattgatt ttcagcacct gaacgtaccc aaccgataca gttagcatgg tcaaagtagt
    11101 cattttgttc tccataggcc aaatcttttc ggatggctag gaggcggtca aggatttctt
    11161 tgaaatcttc ttgagcatac tggcctgaaa tcccatagta gtctccgtaa aagacacatg
    11221 gaaggccgtc ttggcgtaac aaaatgaggg cataggctgc tggcttgaac cattcttcaa
    11281 cggtagactc aagggcttgt cctcgttggg tatcgtggtt gtcgacaaaa gtcacagcct
    11341 tgtcaggctt gagttcaacc aggctatctg tgaaaatgcc acgaaggtca tagtttgcgc
    11401 cagcttgact ggcttcaaag agattctgat ggagacgaac atcgacaagg tcaaagtgtt
    11461 cttccgtttt ttcgagatag tccagattgg cttccttgtc tgggttccaa aattcaccaa
    11521 aaacatagaa atcgtcaccg tatttttcct tcatatcgcg gatgaagttg cgcataaaga
    11581 aagagtcaat atgcttaacg gcatccaaac ggaaaccagc tacaccagtc gtttccatga
    11641 accaatcagc ccagtcatag atgttttgga tgacttcagg atgtttaaag tctaggtcgg
    11701 catacatgag gtagtcgtag tttccgtttt cgttatcgac caattcctcg ttagcccagc
    11761 ccttgttgtc cccttggatc agataaatcc cagacttacg gcgtttggca tcatagtctg
    11821 tacctgtgaa gtggtaccag tgccagtgga agtcattgta ggtatcttgg cgaccatcga
    11881 aggtaaaact agtccagcca ttgatggtga agggttctcc aagttcaact gtacggtcta
    11941 caggatccac ttcaataact tgaaaggctt ccctgtgatc agcagcagcc ttgtggttga
    12001 gaaccacatc ggccataggt tgaattccct gcgctttaag ggcttgaatg gcttgaagat
    12061 agtcttcttt gaaaccatac ttggtgcgga cagtcccttt ttggttgaac tctcctaagt
    12121 caaataagtc atagacccca tagccgacat ctttttcgtt ggttgccttg aaggctggtg
    12181 gcatccagac gtgactgatc cccagatgag ctaggtgtgg agcattttca gccagacgcg
    12241 tccagtgttg accgtcgtgg ggcagatacc attcaaagta ttgcatgagt gtttgatttt
    12301 gcataatgtt tcctcttact tgtcaatctt gttctttatt ctatcataaa gtatctatta
    12361 gcgcaaacgt ttgcgtaaga tagaaggaaa ctattttaac tacttaaaat aaagtattga
    12421 tttttttgta tcaaagtgct agtatagcga tatataatac agataaagat aggagttgtc
    12481 ccatgattga atttcaaaat gttagtaagt tgtatggtga taaaaaggcc ttgagtaatc
    12541 tcaatttgca gattgaaaat ggagagatta tgggcttgat tggtcataat ggggctggaa
    12601 aatcgaccac tataaaatcc ctagtcagta tcatttcacc cagcagtggt cgtattttgg
    12661 tagacggtca ggagttatcg gaaaatcgct tggctattaa acgaaagatt ggctacgtag
    12721 cagactcgcc tgacttattt ttacgcttaa cggccaatga attttgggaa ttgatcgcct
    12781 catcctatga tctgagtaga tctgacttgg aggctagtct agctaggcta ttgaacgttt
    12841 ttgattttgc tgaaaatcgc tatcaggtta ttgaaactct ttctcacgga atgcgtcaga
    12901 aagtctttgt catcggagca ctcttgtctg atcccgatat ttgggtcttg gatgaaccct
    12961 tgactggttt ggatccccag gctgcctttg atttgaaaca gatgatgaag gaacatgcac
    13021 aaaaagggaa gacagtcttg ttttcaactc atgtcctaga ggtggcagag caagtctgtg
    13081 atcggattgc cattttgaaa aaggggcatt tgatttattg tggtagtgta gaggacttga
    13141 gaaaagatta cccagaccag tctttggaaa gtatctacct tagtcttgct ggtagaaaag
    13201 aggaggttgc ggatgcgtct caaggtcatt aaaaaattag ttgatatcaa tatcctttat
    13261 tcatctcaag aagctaatct ggctaatcta cgaaagaagc aggctaagaa tcctgggaaa
    13321 aaagtaaatg tttccgctag agtcttaagt tcttacattt tttccagtct cttgatgatc
    13381 atctgtttta gtaatatagc cattcatttt ccttttgagg aaatacctat ttattttagc
    13441 ttgatgattg ctattttact agtgattgcc ttttcaactt ctttaacggc cttttacaat
    13501 gtcttttatg agagtaagga tttgatatcg tataggccct atgcctttaa agaatcagag
    13561 attataattg ctaaaggact gtctgtcctt ttgccagctc taactggaat tgtaccaatc
    13621 ctagcttatt ttctggtcct ctacattagg ctagctcctt ctctatggct gggtttgcct
    13681 ttgatgctac tgtccttgac cttattattt gtctctgtgg ctctagtgat ggtagtggca
    13741 gtgcatttct tggctcaaac tagggtcttc agaaagtatc agtctatttt ttcgaatgtg
    13801 atgattggga taggagttct cataccttta atatttatct tctttcttca gtcgactttt
    13861 ggaagtattg ttgacaaagt tagagacatt ccatttctcc tttatcctct tcatatcttt
    13921 tacaaaatag cagtggatcc tttttcgaca gaagccatat tgggtctgct cgcttggata
    13981 ggactaactc tcttcctgct ttatctgacc aaaaagaagg tccttcctcg tttttatgac
    14041 gtgatcctgc ttaatagtga ggagaaggtc aaaaaagaac gtcgcagcaa ggagagaatt
    14101 tcaaccacta aaaagggatt tttccgcatg gttttacgct accacctcac cctcttggga
    14161 cagggtactg gcgtggttac agtgcttttt acaagtgctt tctttcctta tctcatgatg
    14221 atcggtctga tttccaaaat ccgagattct cagatagttc cagacattca tcctccatac
    14281 tggttaccct tgttttttgt aggagtgttt atagcagttg tcaataacaa tatcaccagc
    14341 ctgcattcaa ttgccttgtc cttggagagg gaaaatgttg attttcttaa gagtttaccc
    14401 tttgactttg ctcgttatgt gaaagtgaaa ttttggatta tatatgctgt ccagtccttt
    14461 ttaccagttc tgactttgct tggtctttct ctatatctag gcttgcccat catttcgatg
    14521 atttacctta ttgtggcatg gatccttgcc agtgtcatcc tttcttgcca ccattacttt
    14581 aaggacgtta aaaatctgtc aaccaattgg agtagcatta cggacctggt gaatcgttca
    14641 aatggcatag tcgccatagt tttattgttt atttatagtg caatcctgat ggcccttgta
    14701 attgggagca tattcttggt tcagtctctc tcccctatcc ttgccatcaa cttgggagta
    14761 ggagctctta tcgtcttgct tgctcttgct atttttggct atcattatta cctgtcacgc
    14821 atattggcag aaatagaaaa aagatgagct ggttggttgc ttgcttgata aattcaattc
    14881 gacaaacgct tttatagttt gttagaatgt agttgtaaat gattaagatt ggtaatcaaa
    14941 aatataggga
//
LOCUS       DAVLYR010000033        14921 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLYR010000033 DAVLYR010000000
VERSION     DAVLYR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.033"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..1605)
                     /locus_tag="VYO63_000817"
     CDS             complement(<1..1605)
                     /locus_tag="VYO63_000817"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1671771.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENRN"
     gene            complement(1995..2582)
                     /locus_tag="VYO63_000818"
     CDS             complement(1995..2582)
                     /locus_tag="VYO63_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1671772.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2710..3159)
                     /locus_tag="VYO63_000819"
     CDS             complement(2710..3159)
                     /locus_tag="VYO63_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671773.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3164..3553)
                     /locus_tag="VYO63_000820"
     CDS             complement(3164..3553)
                     /locus_tag="VYO63_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1671774.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3581..4207)
                     /locus_tag="VYO63_000821"
     CDS             complement(3581..4207)
                     /locus_tag="VYO63_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1671775.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVFASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4221..4784)
                     /locus_tag="VYO63_000822"
     CDS             complement(4221..4784)
                     /locus_tag="VYO63_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1671776.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(4949..5098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5158..5661)
                     /locus_tag="VYO63_000823"
     CDS             complement(5158..5661)
                     /locus_tag="VYO63_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1671777.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(5854..6684)
                     /locus_tag="VYO63_000824"
     CDS             complement(5854..6684)
                     /locus_tag="VYO63_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1671778.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRLLV
                     LIAIFSVFLLYLSEIHFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGAYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6669..8351)
                     /locus_tag="VYO63_000825"
     CDS             complement(6669..8351)
                     /locus_tag="VYO63_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671779.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLRTELVKETPELQSLFRLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKALSSFIQTEGRYLWEGQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEQEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTDPASLLSNPELLVKANLKETSIFNLAKKLDVDPLALTAFYKERREGCKLN"
     gene            complement(8453..8710)
                     /locus_tag="VYO63_000826"
     CDS             complement(8453..8710)
                     /locus_tag="VYO63_000826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671780.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(8717..9265)
                     /locus_tag="VYO63_000827"
     CDS             complement(8717..9265)
                     /locus_tag="VYO63_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1671781.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9280..10128)
                     /locus_tag="VYO63_000828"
     CDS             complement(9280..10128)
                     /locus_tag="VYO63_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1671782.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10316..11665
                     /gene="trkA"
                     /locus_tag="VYO63_000829"
     CDS             10316..11665
                     /gene="trkA"
                     /locus_tag="VYO63_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1671783.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11669..13108
                     /locus_tag="VYO63_000830"
     CDS             11669..13108
                     /locus_tag="VYO63_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1671784.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKIKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13233..14912)
                     /locus_tag="VYO63_000831"
     CDS             complement(13233..14912)
                     /locus_tag="VYO63_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1671785.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSVMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         4466 a         3388 c         2749 g         4318 t
ORIGIN      
        1 atttctgttc tcaaccgctg cagtaacaaa ggcagtgtag agttcttctg gtcggtttgg
       61 acggcttgac agttcagggt gatactgaca agctacaaag aatttatttt caggaatttc
      121 cacgatttct accaaacgat tgtctggaga aactcctgaa aagacaaagc ctgctgcctc
      181 aaactgctca cggaaggcgt tattaaactc ataacggtga cggtgacggc gttgcaccac
      241 ttcttgattg tgataagcag cagcagcctt agagccacgt ttcaacttag acggataaag
      301 tcccaaacga agggttccac ccatatcctc aatatcaatc tgatcacgca tgatatcaat
      361 gatagggtat tttgtttctg gtgcaagctc tgcagaattg gcaccttcaa gacctaaaac
      421 gtgacgagca aactcgatac atgtcaactg cattcccaag cagactccca acattggaac
      481 atcattttca cgcgcatagc ggatggcttg gattttccct tctgtaccac gttgaccaaa
      541 accacctggt acgatgatcc cgtctgcatc agacaagagt tctgctacat tctctgctgt
      601 cacatcattg gcattgaccc aattgatttt aacttctgca tcattgacat agccagagtg
      661 tttcaaggct tcgaccactg agatataggc atcttgcaac tccacatact taccaacaag
      721 ggaaatctta acttgtttct tgaggttcat gaccttgtcc accatggctg accattctgt
      781 catatccgct gctggtgcgt ctaatttcaa atgatcacaa acaatttggt ccatcccttg
      841 tgcctgcaag ttcagtggaa tttggtaaag atgttcaaca tccaacgatt cgataacggc
      901 ttctggtgcc acatcacaga actgggccag tttattttta attccttgac cagctggctc
      961 ttctgtacga ataaccaaca tatttggttg gattcccaag ccacgcaatt ctttgacaga
     1021 gtgttgggtt ggtttggttt tcatttcacc agcagccttg aggtaaggaa gcaaggttgt
     1081 atggatatac atgacattat ccgcacccac atctgccttc atctgacgaa gagcctctag
     1141 gaatggcaag gactcgatat ctcctactgt tccaccaacc tctgtgataa tgacatcaga
     1201 gtcggtcgtt agagcggcac gcttgatttt ttctttcaaa gcatctgtga tatgaggaat
     1261 gacttgaaca gttgccccaa ggtattctcc acggcgttct ttacgaagaa cttcactgta
     1321 aattttccca gttgtcacgt tggaatattt gttgagattg atatcgataa aacgttcata
     1381 gtgacccaag tccaaatctg tctcagctcc gtcatctgtc acaaaaactt ccccgtgctg
     1441 gtaaggactc atggttcccg gatcaatatt gatataaggg tcaaactttt gaatggttac
     1501 tttgagacca cgatttttca agagacggcc tagactcgct gccacaatcc ctttcccaat
     1561 ggacgatacc acaccaccag ttacaaaaat atatttcgta gacatagatt cctctttcta
     1621 aaatgctcaa ggtcttgtta actacgaggg gatatagaaa acaagaccct actaataaga
     1681 ataatctggg acgactgcac cagattcaca actaatactc ttcgaaaatc tcttcaaacc
     1741 gcgtcaacgt cgccttgccg tagatatgtg ttcctgactt tgtcagtctt atctacaacc
     1801 tcaaaacagt gttttgagca gcctacggct agtttcctag cttgctcttt gattttcatt
     1861 gagtataaaa tacaagaaga taacttcttg aaaaaacaaa aatagctccc taagaactag
     1921 ggagccccga cctctaaaag aggtgcccaa acaatatgat acctaaaaat aggataattg
     1981 tcaatagcta actcttattc ctcgctggtt tcagcgtcgt catctgaaaa ctcgtcgtcg
     2041 tcttcgttga gatccacatc ttctcccaag tcatctggag cgatttcgtt gatttctgca
     2101 tcataagctt ccacttcatt tttttcatca tctggatttt catcatcgta tgaaagagct
     2161 ggatctgctt cgtatgcatc ttcgtcttct ggatcatctg cattgtagtc aatggcatct
     2221 gaatcaccat ccataaaggc attgacacgt tttttcttag cttttggtgc tacttcatcg
     2281 tcgtcatttt cttcaagagc gatgatttct tcgtcgattt cgtccacacc ataccatgaa
     2341 cgaagacccc atttgttgtc cccaagtgag atgaagctac cgtcaaagtt caactctgtg
     2401 tagaacaaag gcaaagcttc gcggatatcg ctgtttgatg ttccaaggta gttttgaatt
     2461 tcgtttacaa gatcgctaaa atgcatctca tgatcgcgac cacgaagttc caagatagca
     2521 cgcgctacct caatcataga tagttcactt ttttcttgcc cagcaaatac ttctaattcc
     2581 aaagcgtttc tcctcattta tactactatc gccagagcga acagactctg acctcatttt
     2641 atcatttact ctttatttta cgataatttt gcggaatagt caaaggttaa gggggagaaa
     2701 gtggcaggat tagactaatt ccaatataaa actccttcct ttttctgttg ctccattttc
     2761 cacaaatcca agcgacttga aacacctcct agaagcatga ttgtaggtgt agatttcctt
     2821 gactctcaat tctttccatc cttttactcg agccaattca atcaaagcac ttagaatctt
     2881 ttttccaagt cctcgatgtt ggtaagcgga attcccaatc acaatgggga gattatcctg
     2941 agatagtgta atatccccaa ttggaaacca ttctcccttc tccttgactt caatccaaaa
     3001 aagctcacca tgccgatcca aataggaata catggcttcc aaggtcgctt gactgtaagg
     3061 aagcttcacc ccatctacga ggtaaaccaa gttcacatcc tgataccaag caaaagcttc
     3121 ctcacaatga ttgaccttat aataaggaac aaaacacaat gcactacctt ttcaacaaaa
     3181 gagttgctgc ttgattaaaa ccatcacacc agttatacca ttttgcttca tacccatctt
     3241 gagctaggat acgatcttct aaatcaaaaa cagagtaaat ctttctttcc tcgcaagctt
     3301 gcgcatagag atgatatagt tcatcaccac catctctatc ccactcagca gaaatcgtat
     3361 cccgacctgc caataaagcc tgataagccc tgtgatgccc atctgtaatc agcaaacaat
     3421 ctccaaaggc aagaatactg attggatcga cttggtttgt ttctgccgac tggtaaagca
     3481 tctgaatatc ttgcaacttc ttttctgata aatatagttg agtcggatga agatctgcta
     3541 tattgacttt catttctttc tcctcaaggg aattcgatac tcacttctgt ttgcctttaa
     3601 atcgccattg gaagcggagc ttgtcataga agggaaactc gataaacagg actcccaagc
     3661 ccacacagag actggcaaag acgtctgatg ggtaatgaac ccccagatag actcttgata
     3721 ccagcacact gactaggtag aggccaagga cgatttgtac gatttttctc cagactggat
     3781 ttttaattcg ttgactgaga atgacaatca gagtacctac catcaaagtt acagccagag
     3841 aatgtccact tgggaaggaa aatcccttct cttccaccag atgtaagata gctggtcgtg
     3901 ggcgctggta gatattttta aaggtcacga ttaaaagacc tgccaaagcc agatttccca
     3961 gcatgaagaa actttctatc ttccatcgct tacgataaaa gacaaaagct gtaatgacaa
     4021 cccaagtgat aatcactggg atatcaatca gacgtgtgag ggctcgaaaa agaatagtca
     4081 aataatctgg taagtctcct cgaatggcag tctgaatcga ttggtcaaaa ttgaccaaca
     4141 tttcagggta aaatttgacc atatagccaa gaataacgaa aagtaaaagg gcaaaactgc
     4201 ccttcattaa aaatgtttgt ttatctttca tagtgtttta aggttggttt caaaaggacg
     4261 tacaacaacc agaatgaaac ggaaaagatt acaccctcaa tcaagttaaa aggtaatacc
     4321 atggtcatta ggtagttgga aagtcccaaa atttttccaa tatcaaagtt agcaaactta
     4381 gcgtacaaag gaacagcata aacatagttg agaaccaaca tggccaaggt taaaccaaca
     4441 gttccagcta gagagcctag taggaaacga agggttgtcc gttccttttt ccaaatcaaa
     4501 gcaaatacga tgacaaaaac tcccaaagct acgatattca tcggcaaacc aatgtaagta
     4561 ttcactcctt ggctgttaag aagcaatttc aatagtgagc gaagcaagag cactcctaga
     4621 gcagcaggca aatccatgac caccagaccc acaaggactg gcaagatact aaattcgatc
     4681 ttgaggaaag atgccgctgg taaaagcgga aagtcaaagt acatcagcac aaatgagatg
     4741 gctgataaaa ttgcaatggt cgaaagtcga cgtgtgtttg tcataacagg ttcctccaat
     4801 tttctataaa atcagaagaa gttggaaagg attcctctat ctattctcac tttttatatc
     4861 ccaaaaattc cctcttactc tattaaagaa aaacaaagca agtggttaca atccggctat
     4921 aaatctatca aaacagacaa agctattctt tcgtcttctc ccatccagac tatactgtcg
     4981 gttgtggaat ctcaccacat cagcttgcgc tcgcggactt ctttaaagag aaggaaaaga
     5041 tcatttccta gttaaagtca ccgccggtcg ggaatcacac cctgccctga agactctttt
     5101 atcataacaa aaaacgcttg caagcgcaag cattttgttc attttcattt tcatttttta
     5161 tttcaattta tccacttcat aggtatggac gagctcaaga cctgcaaagt tctgctggcg
     5221 aagagcctca tagacaatca tgcagacggt attagacaca ttgagactgc ggacatgttc
     5281 atcattcatg ggaatacgga gagccttctc aggatgttct cgcataaaat cttcaggtaa
     5341 gcccttgtct tcacgtccaa agagaaaata atggtcctcg tcagtcgata aatccacctc
     5401 agaatagact ttttctgcga atttcgaaat cagatagagt tttcccttca tctgagatat
     5461 gaaatcttcc aaactctcgt aaaaataaat ctctagctta tcccagtaat ccaatccagc
     5521 ccgcttcatc ttgcggtcat caataggaaa gcccataggc ttgatgatgt ggaggggaga
     5581 attggtcgca gcgcaagtac gcgcgatatt gcctgtattt tgtggaattt gaggttcaaa
     5641 taatacaatg tgatttgtca tgacttgctt cctttcacca ttgcaaaaaa atagccacac
     5701 tgcccggagt caagctcagc aaacagcgtg gttaaggcat cgttaactta cctcacaaca
     5761 ggtttgaagt aaatcagcga aactactttc ttagtataac actttcagaa tcattgtcaa
     5821 tagaaatgac ttgatttttt caattttttc aagctatttc caagggttgt aaaatcgtcc
     5881 ctgattctgc aagataagta gtaaactaac tactaaaaac aaggttgcca agagcaaggt
     5941 aatatagtct ccttttttca aggcctgata actataccat gtgcgttttt tctctttccc
     6001 aaagcggcga agctccatgg cagtcgcaat ggtatcaatg cgttctagcg agctaaaaat
     6061 caagggagta ataatgagca gattgccttt gattcgttgc ataagagaag ctttcttgga
     6121 taattccatc ccacgcgctt cctgagacat cttgatggta aagaattctt cctgcaaatc
     6181 tggaatatag cgcaaggtta gactgacaga ataggcaatc ttgtaaggca caccaatttg
     6241 atttaaactg gaagcaaact gactagggtg ggttgtcatc aaaaagataa tagccagagg
     6301 aatagtgcaa aggtacttaa tagccaaatt tagcagataa aagagctctt gactggtcag
     6361 agtgtaggca ccgattccct gccaaatcac acttctctct ccgtaaagtc caaccccata
     6421 ctcgggagaa aagagataga ccatcaaaac gtttaaaacg gcaaataccg tcgcaaaaac
     6481 ggctacaaag gaaacatctt taaaatggat ttctgacaaa tagaggagaa agaccgaaaa
     6541 gatggcaatc agaacaagca gtctggtatc atagctaatc atggccgcca atgacacgag
     6601 aatgaaaaag aggagtttcc cagcgcctga caagcgatga atcacagtat ctctatgctg
     6661 gtaaccgatt aatttagctt gcatccttct ctcctttctt tgtaaaatgc cgttaaagca
     6721 agtggatcca cgtcgagttt cttagccaag ttgaagatag aagtctcttt taggttggct
     6781 tttactaaca gctcaggatt gctcaacaga ctagctggat cagtatcagc aatcaattcc
     6841 ccatccacca tgacaagggc tcgatctgaa taatccagca tcaattgcat atcatgggta
     6901 atcatgacaa tggtatgccc ttgttgatgc agttcttcga gaaattccat aatctcagta
     6961 tagttcttct ggtcttgacc cgcagtcggt tcatctagga ggataatttc agcgcctaag
     7021 accaaaattg aggcaatagt cacacgtttt ttctgaccaa atgacagggc agaaatgggc
     7081 caattacgga attcatagag accacagatt ttcaaggttt catagactct cgtttcaatt
     7141 tcctgctcgt ccacacctcg caaacggagt cccagagcca cctcatcaaa aatcatattg
     7201 gttgaaatca tttgattggg attttgcagc acatagccta ctcgttccgc ccgctctgca
     7261 acagaatctc cttttatatc ctgtccttcc caaagatagc ggccttcagt ctggataaag
     7321 ctactcaagg ccttggctag agttgacttc cctgctccat tttttccgac aatggcaatc
     7381 ttttcaccct ttttaatatc taaatgaaga gattttaaaa tcggtctatc atcataagaa
     7441 aacgacacgt cctctagtct aaagagtgac tgcaattctg gggtttcttt taccagttcc
     7501 gtccgcaact gaacctgacc ttttgagata gacaagttat ccagatctgc taattgttct
     7561 tccttgacta aatccacacc caattgacgg agagtggtga gataaagggg ttcgcgaatt
     7621 ccattttggg tcaacaaatc agtcgccagt aactggtcag ggctcccatt aaaaaggata
     7681 cgaccatcgt ttatcaagac aatccgatcc acagggcaat gcagaacatc ctccaaacgg
     7741 tgctcgataa taagggtcgt cgtcccctcc tccttatgaa tctggtcaat caattcgata
     7801 atatcctgac ctgacttggg atcaagattg gcgagtggct catcaaacaa gagaatcgga
     7861 ctttcatcaa tcaagacacc agccagactg actcgctgct tttgtccacc tgacaaatcc
     7921 tgagggcgct gatccagtaa aggaagaagg tccagctttt cagcccattt ataaacacga
     7981 cctttcatct catctagggc tgtcacatca ttttccagag caaacgccaa atcttctgcc
     8041 acagacaagc caataaactg cccatctgta tcctgcaaaa ctgtgctaac cagatgagac
     8101 ttatcataga tgctcatatc aaaggctgtt tgacccttta tcaaaaattc tccatatgtc
     8161 tgacccttgt aaatattggg aataatccca ttcaaacact gacccaaggt agatttacct
     8221 gatccagatg gtccaacaat taagactttc tctcccttgt aaatggtcaa gtctatccct
     8281 tgcaaggtcg gttcttgttg tgtttcatac cggaaagaga aatccttcca ctcaattata
     8341 gcttctttca tcttactctc ttcattcgct tcttagactt ctattttatc ataaatcaag
     8401 cccttcttgc agtctcttct cttaaaatct tagcgccaaa aagattccta tcctagctta
     8461 cttgcctaac taatctataa acatcgaaaa agactagttg cccagccttc cccatcattt
     8521 tatactcttc gaaaatctct tcaaaccacg tcagcttcgc cttgccgtag gtatggttac
     8581 tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact tcgtcagttc
     8641 tatccacaat ttcaaaacac tgttttgagc aacctgcggc taacttccta gtttgctctt
     8701 tgattttcat tgagtattag tcctttttca aacttcctgc acgagtttgg gttcctgcat
     8761 aggcaagtaa gagaagagtt cctgcaatag ctacagatac accattggca attcccgcaa
     8821 caatcccttg tgcaaatact ttttctgccg cttcttgata aatcacaaca tctccaagtg
     8881 gtgccaagac accccaaaca agggcatttg caagtagttg aatgaggtta aaaataagaa
     8941 tatctttcca gtcaaaaaca ccattgatca cgcgaacgta ctttctaaaa agtcccacaa
     9001 ctaaaccaaa gagtccgcta gcgataatcc aagtccacca tagaccataa ccagcaagag
     9061 agtccttgat tgcatgacca atcaacccga caagcaaacc gataatcggt ccaaaaataa
     9121 tagaaagtag cgcttgtacc gcatactgaa gctggatgct tgtatttgga acaggggttg
     9181 gaatgttgat catcccgatg acgacaaaga gggcagcgcc aattccgaca gcaacaactt
     9241 gtttaattgt aaatttaatt tccatactat tctcctattt tatccttcta ttttctttat
     9301 ttcaatggtc caagatgaac cgacacctac attataggcc ttggcaaagg aaccttggtt
     9361 gatagccaaa cctaaacgat agagagagtt gatgtaaagg atgggttgcc caattctcac
     9421 atctgcaaat gatttgccat agacaacctg attttgatag accagcatat cagcatgata
     9481 gatggtcact tcaaaacgat caccaaattc tggttccagc ttgtaaaatt cttcccgtgt
     9541 gatagaggtc caaagcgaac cgaaacgcac atccagaata tcaatggctc ccttcaccag
     9601 atgatcttct atgatggtcg ctacgactgg aagctctaca atctgttcca cactgagctc
     9661 tggccctact tcctcaaaag taatgtgacc actggccagt ttagcaccag tataggcata
     9721 gacatcacga ccgtggaagg tataagaatg ctctgtgttt tgacgcctat tggccacctc
     9781 agaaatctca cgaatggcta caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt
     9841 atctggcgtg acaatgtatt gattttttgc agtcttggca actacactct tacgtttcga
     9901 accgacacct ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt
     9961 ctgaaagaga cgatagctcc cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg
    10021 atggattttt aaggttggag actcttctaa agccactcca atcatagccg ataccgcacc
    10081 atcaaccaga ccaaagtctg attgtaatac cagtaaatta ttattcattt tatctccttg
    10141 tatatccttt atcataccat atttcagaga aagtcgctaa tagctatttc aattggttag
    10201 ggtatagttt taccttatag cgaattttca ctaaaagctc ttgttattag ctagtaggtg
    10261 cattttttct tgaaaaaggg tatgatagtt acatcatgaa aaagtaggtt ttattatgaa
    10321 aattatcctt gtcggagggg gaaaagttgg ttttgccctc tgtcgctcct tggttgcaga
    10381 aaagcatgat gttttgctga ttgagcaaga cgaagctgtt ctcaatcata ttgtcagtcg
    10441 ctttgatatc attggtatcc ttggtaacgg ggccgatttt gccattcttg agcaagccag
    10501 cgtccaagat tgtgatatct ttatcgccct gactgagcac gatgaagtca acatgattgc
    10561 agcagttcta gccaagaaaa tgggagctaa agaaactatc gttcgggtgc ggaaccctga
    10621 atattctaac tcttatttca aggaaaagaa tattctcggt ttttctctta tcgttaatcc
    10681 tgagctcttg gctgcccgtg ctatcgcgaa tatcattgac ttccccaacg ccctgtctgt
    10741 cgaacgcttt gctggtggac gcgttagcct catggaattt gtcgtcaagt ccaccagcgg
    10801 tctttgccaa atgcccattt ctgattttcg taaaaaattt ggtaatgtca ttgtctgtgc
    10861 gatagagagg gatcatcaaa ttatcattcc aagcggtgac atgactgtac aggataaaga
    10921 tagaatcttt gtcactggta accgtgtcga tatgatactc ttccataatt attttaaatc
    10981 acgcgccgtg aagagccttc tcatcgttgg ggcaggtaga attgcctatt atctacttgg
    11041 tattctcaaa gatagtcgta tcgatacaaa agtcattgaa atcaatcctg aaatcgccag
    11101 cttctttagc gagaaattcc caaatctcta catcgttcaa ggagatggaa ccgcaaaaga
    11161 tatcctgctg gaagaaagtg ctcaacacta tgatgccgtt gcgactctaa caggagtcga
    11221 tgaggaaaat ctgattacat ctatgttcct tgacagggta ggtgtacaga aaaatattac
    11281 taaggtcaat cgtaccagtc tcctcgagat tatcaatgcg cctgattttt caagtatcat
    11341 cacacctaaa agcatcgctg tagatacgat tatgcacttt attcgtggtc gagttaatgc
    11401 ccagtattca gaccttcaag ccatgcacca tctagccaat ggccaaattg aaaccctgca
    11461 attccatatc aaggaagcca ataaaatgac tgccaaacct ctttctcaac tgaaattgaa
    11521 aaaaggggtt cttattgcag ccattattcg aaagggcaag actattttcc caactgggga
    11581 ggatatgttg gaagttggag acaagctcct agtaacaacc ttgttgccaa acatcaccaa
    11641 gatttatgac ttgattgcga ggtaagaaat gaataaaagt atgattcgtt acctcctttc
    11701 aaagttactt ttgattgaag ctgttctcct cttggttcct gtggctatcg ctgtctatta
    11761 ccgtgaatcg agccaagtct ttacagccct cttttcgacc atagggattc tcgtattgtt
    11821 aggcggttca gggagtttac agaagccaaa aaatcaacgg atttatgcca aggagggagt
    11881 cttgatcgtt gccctctgtt ggatcctttg gtctttcttt ggtggtctcc cctttgtctt
    11941 ttctgggcaa atccccagcc ttattgatgc cttttttgag attagttccg gctttacgac
    12001 tactggagca agtattttga acgacgtttc agttctcagt cgttccctcc tcttctggcg
    12061 aagttttacc cacttgattg gagggatggg agtgcttgtt tttgcacttg ctattatgga
    12121 caatgccaaa aatagccacc tagaggtgat gaaggctgag gttccaggtc ctgtttttgg
    12181 caaggttgta tccaaaataa aaaacactgc ccagattctc tatctccttt atctagctct
    12241 cttctccctc tttgtcatca tctattatct agctggtatg cctctctacg atagttttgt
    12301 cattgctatg gggacagcgg gaactggagg ctttaccgtc tataacgatg gaattgccca
    12361 ctatggtagc tcactgatta cctatctggt tagtatagga gttttggttt ttggggtaaa
    12421 tttcaatctc tactactacc ttatgctccg tcgggttaag gccttctttg gagatgaaga
    12481 gcttcgggct tacttggtca tcgtgctggt ttctacaggc ttgattagcc tcaatactct
    12541 ctacctctac ccaggatttt ccaaaagctt tgaaatggcc ttcttccagg tttccaacat
    12601 cattacaaca actggttttg gatatggaga tattaccaac tggcccctct tctcccagtt
    12661 tatcctcctc ttcctcatga caatcggtgg ttctgctgga tcaactgcag gtggactcaa
    12721 gattattcga ggcctcatcc tttcaaaaat tgccaaaaat caaattttgt ccattctatc
    12781 tccccaccgt gttttgactc tccatgtgaa taaaacggtg attgataagg atacccagca
    12841 taaaattctc aagtactttg tcatctatgc tatgattttg ctatccctta tctttattgt
    12901 cagcctagat agcaatgatt ttctgatcgt gaccagcgct gtctttagct gtttcaataa
    12961 tatcgggcct attctaggaa ccacttctag tttctcaatc tttagtccta tctcaaaaat
    13021 tctcctctcc tttgcaatga ttgcaggccg cttggagatt tacccaatcc tccttctctt
    13081 tatgaagaga acttggtcta agagataaaa tacaaaatcc ctctttttac aattgtgaaa
    13141 gagggatttt tatattaaaa agggggaacg tttttaaatc caagcctgtc tgtaagcaaa
    13201 gtccttactc aaataaaaga tggaaaaact tactacacag cttggtaatg ctccaataca
    13261 aattgtaagg ctttacttgg attctttgtt aaatgaatat attccattcc tctggtagca
    13321 actatctgaa tacctaatct ttctgcatcc accttcatct ctctataata actccgaact
    13381 tgtggagcca gaataattaa atcataaaca cccataatat cataatgagc tccgtacgct
    13441 cctgaattcg caatcactct aacttctgct aattgagcgc cctcgttaat tgcattggct
    13501 aattgcgcac ttgttccaga ccctgcacaa agaaccagta ctttcaactc cttacttttt
    13561 atttcatcta actcaccagg cattatttca cttgtctcct cctctaaaat aatatcattt
    13621 gagcttgcaa catcttcttt catcagtaac tgtctatcat acgctctaca gaatggcaaa
    13681 taaatcaata tgtcgacaac taaaatcaaa gataaaaata caaaggagat agattgaaaa
    13741 ttcgttccaa ttaacaatcc caagggacca ggaaaggccc aaggtaactg gatataaaat
    13801 ccattcattc caaagaaatc aataaagacc tttcctagaa atacattcac tggtggagtc
    13861 atcaaaaaag gtacaaaaag atagggattc aaaataactg gcataccaaa tagaagaggt
    13921 tcattcaccg caaataaaac aggagtaatt gtagctttac ctaccgattt taattgttta
    13981 gaccgcataa agaaaatcaa aataaatggt acaacaaagg tagcccccgt tcctccgata
    14041 gcagcgatat aatttccgaa attaggcgtt aaagaatgat aaggaaactg cccatttgca
    14101 agaagttgag cattctcttc catattgctc agttgtaaag ctgtaacagc aggtaagaca
    14161 atagatggtc catgaattcc aacaaaccaa agcaaagcgc acataaacca aatcaacatc
    14221 atagcaggat atgattctgc ccccttaaaa ataggagtca atagttgttg aaatacttgg
    14281 gcaaaaggaa catctaaact aaaccgagat acaatatcta acaaaccact aataagtaaa
    14341 acaaaagaaa aaggaaaaat atctctaaaa gcttgtgata tagccccagg aacttcctta
    14401 ggtaaatgaa tagtaatgtc tcgcttaata cagaatcggt aaacatttac agtaataaag
    14461 gcactgacaa acgaagttat caatccttga gatcctaaat agtttgtcga caaacttgta
    14521 gatcccgtct tctcatcaac tacaagcgtt acagttaata ccagataact acatatggct
    14581 gccaacatta cagaagtatc atttattacc tttccaggag gcatttttct attaacattt
    14641 ccagccaatg actttgcaac tgtccctgaa acaataacac ccaaaactcc catggttagc
    14701 atatacactt tcctcatcca ggcaatagct ggactcggta actctactcc gaaattttta
    14761 ggaatcataa taataatcat caaaaagctt gaaaacataa ttaaaggcat agtagcaatg
    14821 aatccatctt taatagccat catataatta ttagtagaaa ttttttcaaa tttcccttgt
    14881 tgcttttcaa tggcagcgac tagcttatcc ataaaactcc t
//
LOCUS       DAVLYR010000034        14835 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLYR010000034 DAVLYR010000000
VERSION     DAVLYR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.034"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            78..1796
                     /locus_tag="VYO63_000832"
     CDS             78..1796
                     /locus_tag="VYO63_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1671786.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            1907..2254
                     /locus_tag="VYO63_000833"
     CDS             1907..2254
                     /locus_tag="VYO63_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1671787.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFVGL"
     gene            complement(2533..3369)
                     /locus_tag="VYO63_000834"
     CDS             complement(2533..3369)
                     /locus_tag="VYO63_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671788.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(3382..4011)
                     /locus_tag="VYO63_000835"
     CDS             complement(3382..4011)
                     /locus_tag="VYO63_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1671789.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(4021..4662)
                     /locus_tag="VYO63_000836"
     CDS             complement(4021..4662)
                     /locus_tag="VYO63_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1671790.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(4808..6037)
                     /locus_tag="VYO63_000837"
     CDS             complement(4808..6037)
                     /locus_tag="VYO63_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1671791.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(6027..7193)
                     /locus_tag="VYO63_000838"
     CDS             complement(6027..7193)
                     /locus_tag="VYO63_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1671792.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFIAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            7337..8350
                     /locus_tag="VYO63_000839"
     CDS             7337..8350
                     /locus_tag="VYO63_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1671793.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(8395..8814)
                     /locus_tag="VYO63_000840"
     CDS             complement(8395..8814)
                     /locus_tag="VYO63_000840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1671794.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(8825..10231)
                     /gene="atpD"
                     /locus_tag="VYO63_000841"
     CDS             complement(8825..10231)
                     /gene="atpD"
                     /locus_tag="VYO63_000841"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1671795.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(10317..11195)
                     /locus_tag="VYO63_000842"
     CDS             complement(10317..11195)
                     /locus_tag="VYO63_000842"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1671796.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(11211..12716)
                     /locus_tag="VYO63_000843"
     CDS             complement(11211..12716)
                     /locus_tag="VYO63_000843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1671797.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(12731..13267)
                     /locus_tag="VYO63_000844"
     CDS             complement(12731..13267)
                     /locus_tag="VYO63_000844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1671798.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(13267..13761)
                     /gene="atpF"
                     /locus_tag="VYO63_000845"
     CDS             complement(13267..13761)
                     /gene="atpF"
                     /locus_tag="VYO63_000845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1671799.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(13775..14491)
                     /gene="atpB"
                     /locus_tag="VYO63_000846"
     CDS             complement(13775..14491)
                     /gene="atpB"
                     /locus_tag="VYO63_000846"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1671800.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNILEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(14526..14726)
                     /locus_tag="VYO63_000847"
     CDS             complement(14526..14726)
                     /locus_tag="VYO63_000847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1671801.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         4576 a         3276 c         2692 g         4291 t
ORIGIN      
        1 agataaggtt ttactaaaac taaaaaaaat gatataatca aactgattaa atgttttaaa
       61 aataaaggag aatttgaatg tcttaccaag aaaattacca gaaatgggtt gattttgtgg
      121 agcttcctga ctaccttcgt caagatttgg aaaatatgga cgaaaaaact aaggaagatg
      181 ccttctatac aaatcttgaa tttggtactg caggtatgcg tggcttggtt ggtgctggta
      241 caaaccgtat caacatctac gttgttcgcc aagctactga aggattggct cgtttgattg
      301 agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat tgcctacgat agccgtcact
      361 tctcacctga gtttgccttt gaatctgcgg cagttcttgc taaacacggc atcaaatctt
      421 acgtatttga aagccttcgt ccaactccag aactatcatt tgcagttcgt catctcaact
      481 gtttcgcagg tatcatggtc acagccagcc acaaccctgc tccatttaac ggttacaagg
      541 tttacggtga agacggtgga caaatgcctc cacacgatgc ggacgctttg actacttata
      601 tccgtgcaat cgaaaaccca tttgcagttg aagttgctga tgtggaaact gaaaaagctt
      661 ctggcttgat tgaagttatc ggcgaagctg ttgacataga ataccttaaa gaggttaagg
      721 acataaacat caacccagcc ttgattgaag aatttggtaa agacatgaag attgtctaca
      781 caccacttca tggtactggt gaaatgttgg ctcgtcgtgc tcttgcccaa gcaggatttg
      841 actctgttca agttgttgaa gcgcaagcaa ctgctgaccc agacttctca actgtaacat
      901 ctccaaaccc agaaagccaa gcagcctttg cccttgctga agaacttggt cgtcaagttg
      961 gtgcagatgt tcttgtcgca actgacccag acgctgaccg tgttggtgtt gaagttcttc
     1021 aaaaagatgg cagctacctc aacctttcag gtaaccaaat cggtgctatc atggctaaat
     1081 acatcttgga agctcacaaa aacgctggaa ctcttcctga aaatgccgct ctctgcaaat
     1141 ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag ctacggcgca actatgttca
     1201 acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca agaattcgaa gaaaaacaca
     1261 accacactta catgatggga tttgaagaaa gcttcggtta cttgattaaa ccatttgtac
     1321 gtgataaaga tgccatccaa gccgttcttg tagttgctga acttgctgcc tactaccgtt
     1381 ctcgtggttt gacacttgct gacggtatcg aagaaatcta taaagaatat ggctactacg
     1441 cagaaaaaac aatctctgtt actctttcag gtgtcgatgg tgctgaacaa atcaaagcga
     1501 ttatggctaa attccgcaac aatgctccaa cagaatggaa cgcaacagct atcactgtcg
     1561 tagaagactt caaggcacaa actgctactg ttgctgacgg tactgttaca aacttgacaa
     1621 ctcctccaag tgatgtgttg aaatacacac ttgctgacgg ttcatggatt gccgttcgcc
     1681 cttcaggtac agaaccaaaa atcaagttct acattgcagt tgtaggggaa accaacgaag
     1741 aatcacaagc taagattact aacatcgaag cagaaatcaa tgcatttgta aaataaagac
     1801 ctataaaaga tgagaccaac caacctcatc ttttttcgta tcaaatctaa gcaattttag
     1861 aaactttatg gcatactaga cttatcaatg aaagcgaggt aatctcatgg aacaattttt
     1921 agataacatc aaagaccttg aagtcactac agttgtgcgt gcgcaagaag ctcttgataa
     1981 aaaagaaact gcaaccttct ttatcggtcg caaaacttgc ccttactgcc gtaaatttgc
     2041 aggtacattg tcaggtgtcg tagctgaaac caaagctcac atttacttca tcaatagtga
     2101 agaaccaagc caactcaatg atttgcaagc attccgctca cgttatggaa tcccaactgt
     2161 accaggtttt gttcatatta cagatggaca aatcaacgtc cgttgcgact cttcaatgtc
     2221 agcacaagaa atcaaagatt tcgtaggatt gtaaaaaatc agctaacaag tcttaaaatc
     2281 acaaaaacgc ataatgtaag gtgcaaaaac cttgatatta tgcgttttat tgttcctaaa
     2341 acagttacat cagtatttca ttacagaaca ttttaatttt ggaagtcaaa tcaattttta
     2401 acaatgtttt agaaatcaca gtgggctatt ccatattcaa ttatccagat tcaatcgcta
     2461 ttatcgtttt cctagattac aaaaaaaact acgttttgct aatcaaaacg tagtctcagc
     2521 cactatttta tcttactgtc cttcttttac ttctttggtt gctacatctt ctccaaacca
     2581 tttttggctg atttcttgga acttgccgtc cttgtaaaga ctagaaaaag cttcatttat
     2641 cttcttaacc aaggttgtat cttccttacg ggctccaacc gcaaaagctt ctgtttctag
     2701 tccaactgta aagacattat aatcgtttaa aacaccttct gcttctaaat aatagtttgc
     2761 atagacacgg tcaatcaata gaccatcaat tcgatcgttt ttcaaatcaa tcaaggcttc
     2821 attaaaggtt tggtattgat tcgcttcctt attagcgaca atattcttca aaagttctgg
     2881 atttgcttca aagtccgcat aaccagatga accagcttga gctcctaatg tctttccagt
     2941 catatccttt gcagtcgtga taccagatga tttcttcgta accaatacct gctcattctt
     3001 catatatgag ttactgaaag ccaccttttc acggcgttcg tctgtagcgg aatagccatt
     3061 ccaaatcaga tcaatcgttc cttttgtcaa ttcagcttct ttcaaatccc aatcaatcgg
     3121 ttgccaattt accgtgattc cgtatttttc aaaaacagct gtagctaaat caatatcaaa
     3181 tcctgcataa gaaccatctt tctgagcaaa tcccattgga acaaaagtac tatcaaatcc
     3241 aatagtaata gacttgttag actggtactt tgaccaatta tctccactag tttcgctaga
     3301 atttttccca caagctacta agaacaaagc agtcatcaga ctgactaata caagcatcca
     3361 ttttttcatc catttttcct cctatttagg ttctactttc aataatacat ctgcgatatt
     3421 ttcagcaaac tgcaaatcat gggtaaccac aatctgggtc atcccaagtt ccctattttg
     3481 caagattagc ttctccactt ccaaacgtaa ttctggatcc agggcagaag ttggttcatc
     3541 gtagccaatg atttctgggt caatcatcat agcacgcgcc aaagccactc gctgcttttg
     3601 cccaccagat agtgagaaag gataggcctc tgcgtgtcct cctagtccta actgttccaa
     3661 gagtccactc gccttcttct cagcctcttc ctgcttcatt cccatggtct tcacaggcga
     3721 taaagtcaaa ttttccagaa ctgatagatg aggaaatagt tgaaaatctt ggaagacaaa
     3781 tcccagtagg ttgcgcttct gcaattcatc cagctctaaa ggttgtccat tataaaagat
     3841 ttgccctgaa tcaatggttt caagacctgc aagcatacgt aaaagagttg tcttacctcc
     3901 accagaaggt ccaacgatag ccaggatttg cttttcagga atacttagac tgaaattaga
     3961 caggatttgt ttgtctccaa agactttatt gatatttcgt aattctaaca tggcagcctc
     4021 ctatctataa taactatact tcttctcaac ttttttggaa ataattgtca caatcccaat
     4081 caaaatcaaa taaatggctc ctgccaagaa cataggaacc agactagcat cgcggttagc
     4141 agctgttcga ctagccaaga taaggtctga aattccgaga gcatagacca aagaagtatc
     4201 cttgaccaaa ctcataactt cattaaagac actaggaaga acgatcttgg tcacttgggg
     4261 caagataata tagcgcactc tgtcaaaagg gctaaacttc aagaccttgg cagcctcata
     4321 ctgtcctctt ggaatagtgt caattccccc acggaaaatt tctgcaaagt aagccgcata
     4381 attcaaaaca aaggcaataa tagctgcagg aaggcggtct aaacgaatcc caatacttgg
     4441 gagcacataa tagataaaaa tcagttgcaa gagtaagggg gttcctcgca taacccagat
     4501 gtaaatgtta atcagataat ggaggggctt ccaatggact tgcaaggcaa aggcaatcaa
     4561 aacgcccaag ggaatcgaaa atagcaagac cagtgcaaag acctgtacag tcgtgctcgc
     4621 accattcagt aaactcggta atatctcaaa taaataagac atactgcacc tcctaaaagt
     4681 aattgttcct attatagcat gaataaacaa gattaccaag tatttttgta aaaagattct
     4741 accatataca aataaaaacc gtagctatca aaagagataa actacagttc tatatgttta
     4801 aacattctta caatctctca aacagttctt gcatttgcac atcatctgga accagtttta
     4861 agtaagtgtg agcatggact tttgcttctt caaaatgtcc caattcacgc aagagataga
     4921 tatagtgttc cagaaattct ggattgtcct tcaaatctcc tgtcaactct tgataatgct
     4981 cataagcagt atccaaatcg tccatttctt gataagaacg agcaatcatc cacttggtca
     5041 aaagattttc tggctcctca ctctgcaagt ctagaatatc ctcataacgc tcctgctcca
     5101 gataaatagt ggctaaacga agcaagattt cttctgtatc ctcagcgtct tcttttgcag
     5161 taaggagata attttctgca ccactagcat catgcaattc ataagaaaat tgtgaagcag
     5221 ctagcaagag gcgagtttca aagggatttt tctctaatcc ttgcttagcg atacgcaggg
     5281 cttcttgaac ttgatgttcc ttatgtaaag cctgactgta cccatactca tagccttcaa
     5341 agtcaggaga aatggtatca agctgcttaa agtagagggt ggctttttga tattcttctt
     5401 gatcaaaata aagactggcc aactcaaaag ctgttaagtc atcgtattct aactccaggg
     5461 ctttttctaa aaactcagta gccgtttcaa atttccctaa ctgagcatag gcaaagccaa
     5521 ttcgttgata ggtggaaatg cccgtttgct cataaatcga gcgattatct aactgggcat
     5581 aggcttgaat agccgcttgg taattttcca actcactatc caactctgcc aaacccaata
     5641 tcaagagaga atcctctgag taggtcaagg cctccaataa tttctcacgt gccacatctg
     5701 tcaaaccttc cagctggtaa aggtctgcct tcagagccaa agacgagaca taccagtcac
     5761 tgtcagcttg gatttcctca agataggtaa aggcttcttc tatttgacca tcctcgctag
     5821 caattgcagc tagattaaga tgaacctctg gaaaatcctc tacaattttc aggtaaattt
     5881 ccttggcctg aggatagaaa ccaatccctt caagataagt tgccaactca tacagaagat
     5941 cacttgaatc attttctaaa gctttggcga aataatgctc agccttagtt aaatcttgct
     6001 cctccaaagc ctgtaacatc tgttgactat tgttcactct tgacttcctc accctctaat
     6061 tcatatagac cactgactac cttataccat tcgaaaatgg ctgaaatgac aaccttagca
     6121 gaggcataaa ccggaatacc aagtaaaact ccccagatac caaacataga tcctgaagtt
     6181 aacaaaacaa agagaacatt aataggatgg atgtttaatt gacttcccaa aatcaatgga
     6241 gagacaaaac ggccttcaat agtttgttct acaataaaga caatcactac tttcaaaagc
     6301 atgactggac cagcaatcaa acccaatact agggcaggaa gcatggctag aaagctacca
     6361 agataaggga ccagatttaa aataccagca gtaaccccca gcgtaaccgc atagcgtaga
     6421 ccaataatct tgaagaagat gataaacatt actgctacaa taatagccac tgtcacttgc
     6481 cctcgaacat agttggacaa ctgttgattc acatctgata gaacttgtcc aacaggttcc
     6541 ttcaattttc ttggaatgaa ttgggtcaaa tagttacgca agcctttccc atcacgcaag
     6601 agataaaaga gcatgaaagg aacgataatc aaggcaacaa tcacttgaga agctccgcta
     6661 ataaaggcac tcacccagtt gactgcctga gatgaaacct tacttgccaa aactgtagcc
     6721 tggctagaaa aattggtcaa aacttgctct aattgaggtc tgaaatcatc tggcaggtgc
     6781 tgggctacca atccattaac aatcctatct atatcttcta agtaaacagg aacgtttctt
     6841 gcaaaggtca aaacctgacg ttgcagattt ggaatggcga ctgccaagcc ccaaatgata
     6901 aagagagcga tgataacaaa gacaatagtg atagctataa cacgattaac cttatgcttc
     6961 tccatccaat caacaatagg attcaacaaa taatataaca aaccagacaa aatgactggc
     7021 aacatcacaa ctgctataaa gtctaaaaca ggtgaaaata gaaaactaat cttacttaaa
     7081 ataaaaagat tcaatcccaa taataaggtt actaaaaata ccgtaattgc cttgttatct
     7141 aaaaaccact tgaaaaacca agataggcta aaatgtttct ctttttgctc cataaatact
     7201 tcctttctat tgttctctca ttataccatt tttgaaacaa aataactctt ttttaaagtg
     7261 cttacatcta attgaatgtc tttaatttgc ttgtttttgt gatataatag tcttatcttt
     7321 acacagaggt atagtaatga aagaaactgt ttattttgga acttatacac gtcgtacttc
     7381 tcaagggatt tacaaggcag actttgatac agaaactggt cagctttcaa atctagaact
     7441 ttttgcagct gagccaagtc caacctacct tgcctttgac cagcaccaac atttatacac
     7501 tgttggtagc caagacgata aggggggaat tgcagcctat caaactgacg ggactgtgtt
     7561 aaatcatgtt gttgaagaag gagctcccca ctgttatgtt gctgtcgatg aaaagcgtga
     7621 tttggtttac gcagccaact atcacaaggg acaagtcctt gtttataaac gccaggaaga
     7681 tggtagtctt ctacttagtg atatggatca acacagtggc caaggtccac atgaaaatca
     7741 agcttccccc catgttcact atacagattt aacacctgac cactatctag tgacctgcga
     7801 cttgggaact gaccaagtca tcacctatga cctcgatcaa gaaggaaaat tatctaagct
     7861 ctatacctat cacagcaagc caggagcagg ctcacgccat atcattttcc ataaccacta
     7921 taaaatcgct tatctcattt gtgaactcaa tagtactatc gaagttttaa tctacgatgg
     7981 cgttggcgaa tttgaacgta tgcaggttat ttcaacttta ccagaagctt acgaaggctt
     8041 taatggaacc gctgctattc atctctctaa agacggtaaa tacctctacg cttctaaccg
     8101 tggccatgat tctatcgcag tatataccat ccttgcggac ggtagcttag agttattaga
     8161 aatcgttcca acgcatggtc agactccacg tgattttgat ttgacacccg accaaaaatt
     8221 tctcattgtt gtccatcaag actctgacaa tgcaactgtc tttaaacgta attgtgacaa
     8281 tggtcgtcta gcagaactct ccaacgactt ccatgttccc gaagcagtct gcatccgttt
     8341 tgctccttaa aacaccataa aaaatgaact tggtattttc aagttcattt tttcttatag
     8401 tctatttccg acattaatac ggttaatagc acgttgcaaa gcaatcttag cacgacgttc
     8461 ttggtcaatc aaatgtttgt cttgtgcttc ttcaattgca cgttctgcac gaagtttggc
     8521 acgttctgca cgactgatat cgatatcacg agcacgttct gcagagtcag cgacgattgt
     8581 gatcatatca ttggcaattt caataacgcc accgtttact gcaatccagt tcacgtgatc
     8641 tttatcatcg atacgtttta cctttacttc atcaactgct aaaaccgcaa tcatattttc
     8701 atgtcgtggc aagatcccca tctcaccatc cagagttcga accgatacat agctggcatg
     8761 gtgatcatag acgagaccat ctggtgtcac gatctggaca gttaactgag ccatagatca
     8821 cctcttaaaa tcccattttt tcagcttttg caatcacatc ttcgatagaa cctacaccac
     8881 ggaaggcatc ttctggcaag tggtcgtatt taccatcaag gatttcctta aagccacgta
     8941 cagtttcagc aactggaaca taagaacctg gctgaccagt aaattgttcc gcaacgttga
     9001 agttttgtga caagaagaac tggatacgac gggcgcgagc aaccaaggtc ttttcttcat
     9061 cagaaagctc atccatacca aggatagcaa tgatatcttg caattcatgg taacgttgaa
     9121 ggacacgttt tacttcagca gcaactgcat agtgctcttc tccaacgatt tcaggtgcca
     9181 aggcacgtga gcttgaagca agtgggtcaa cggctgggta gatacccaat tgtaccaact
     9241 tacgttccaa gtttgttgtt gaatccaagt gagcgaaggc tgttgctggc gctgggtcag
     9301 tatagtcatc cgctggcaca tagatagcct ggatagaggt tacagaaccc ttcttggttg
     9361 atgtgatacg ttcttgcaat tgacccattt ccgtagcaag tgttggttgg taaccaacgg
     9421 ctgatggcat acgacccaaa agggcagata cttctgaacc agcctgagtg aaacggaaga
     9481 tattatcgat aaagagaagc acgtcttggc cttccacatc acggaagtat tcagcgattg
     9541 tcaaaccagt aagggcaaca cgcatacgtg ctcctggtgg ctcattcatc tgaccaaaga
     9601 ccatggctgt tttctcgata acgcctgatt ctttcatttc ccagtaaagg tcattcccct
     9661 cacgagtacg ttccccaaca ccagtaaata ctgaaatacc accgtgctct tgggcaatgt
     9721 tgtgaatcaa ttcttggatt aagacggttt taccaactcc ggcaccaccg aaaagtccaa
     9781 ctttaccacc tttaaggtaa ggggcaagaa ggtcaataac cttgatccct gtttcaagga
     9841 tttcagaaga ggtagacaac tcatcaaaag ttggagcttt tttatgaatt ggctgacgct
     9901 ctgcgtcttc tgtaaaagga gcttccaaat caatggtatc tcccaaaacg ttgaagacac
     9961 gtcccaaagt ttctttacct actggtacag agattggacg acctgtgtcc aatacttcca
    10021 ttccacgagt caacccatct gttgattcca tggcgatagt acgaaccata ccatctccta
    10081 actccaaggc tacttcaagg acgatttttg tttttctttc gtcatttttg tagacgacaa
    10141 gtgcattgtt aatctcagga agtttttccc ctgctgcaaa caaaacgtct acaacgggac
    10201 cgataacctg agcaatttta cctgaactca tctccttctc ctattctata taagatactg
    10261 tcggttccta gctcaactag gtcctaagtt cattttcata cgagctggac tagagcctat
    10321 tctaaggcac tagcacctgc tacgatttct gtaatttctt gtgtaatcgc cgcctgtctg
    10381 gcacggttat actgaattgt caaatcattg atgactttct tagcattatc tgtcgctgtt
    10441 tgcatggctg tcatacccgc agcattctca gctgtcttgg catcgataat ggcaccgtaa
    10501 atcatacttt ctgcaaactg aggcaacaac tgctccagaa tttcttctcg gctggtttcc
    10561 aattcaaaag tcaagctgta ctcttcatcc gcttcatttg gatccaagtc aacaatcgga
    10621 agcatttgtt ccacacgcat ttgactggtt agcgtattga catggtggtt gtagcaaaca
    10681 taaagctcat caaagagttc attttggtac atttcaacag tttttgaaat aatcttacga
    10741 acttgatcaa agctaggttg gtctgccaag ccacgtaatt cataaagtgg ttgaataccg
    10801 cgagccttaa agaaatcagc tcccatccca ccgatacaga tcatttcaaa acctttaccg
    10861 tctgggtggt attcttcttt caactccata acagctttca aaatagagga attataacct
    10921 ccaaccaaac cgcggtctga agtgataacg atatagcctg tcttcttcac agaacggcta
    10981 atcaacatcg gattagttga agcaccagct ccattaccat gaaggatatc tgtcaaaagt
    11041 ttacgcactt tctgagcgta aacttggaag ttgcgagcag cttcttcaga gcgacctaac
    11101 ttagcagccg ataccatttg catggcatta gtgatttgac tcgtattttt tgttgaggcg
    11161 atttttgttt taatatcatt tagagacact gccatctgac acctctattc ttattggaag
    11221 ctagattgat tgagaaactc tgtaatcgca gcatccaaga ctgcttcttc tggcaagtct
    11281 tttgtatcac gaatggtttc caaaatctct ggatgttgag catcaaagaa ggcatggaac
    11341 tcttcctcga aacgaacaat atcatctact ggaacagtat ccaagaaacc atgtgtcaaa
    11401 gcataaagaa tggttacttg tttctcaaca ggtaatggtt tgtgaacagg ttgtttcaag
    11461 acctcaacgg tacgacgtcc acggttcaac ttagcctgtg ttgctgcgtc caagtcagaa
    11521 ccaaacttag taaaggcttc caactcacgg tatgaagcaa ggtcgatacg aagtgtacca
    11581 gcaaccttct tcatggcttt gatttgtgca gaaccaccta cacgagatac agatgaaccc
    11641 gcatcgatgg ctggacgaat acctgcattg aagaggccat cgccaaggaa gatttgtcca
    11701 tcagtgatag aaatcacgtt ggttgcgata taggctgaga tatctcctgc ttgtgtctcg
    11761 ataaatggta gggctgtaat tgatccacca ccaagttcat cagaaacttt agctgagcgc
    11821 tcaagcaaac ggctgtggag atagaaaaca tcccctggga aggcttcacg acctggagga
    11881 cgacgaagca agagcgatag ttcacgataa gctaccgctt gttttgatag atcatcgtat
    11941 acaatcaaaa catgcttacc ttgatacata aattcttccg ccatagcaac cccagtataa
    12001 ggagctagga agagcaatgg agatggttgt gaagcagagg ctgtcacaac gattgtgtag
    12061 tccaaggcac cgtactgacg aagtgtttct acttgcgtac gaactgttga ttctttttgt
    12121 ccaatcgcga cgtagataca gatcatatct tgatcttttt ggttcaagat tgtatcaatc
    12181 gcaatggttg ttttccctgt ctgacggtca ccgataatca actcacgttg accacgacca
    12241 atcggtacaa gggcgtcaat agctttcaaa ccagtttgca atggttctga aacagactta
    12301 cgttgcataa caccaggagc tggtgcttct actggacgag ttttatcagt gtggatttct
    12361 ccaagaccgt caactggacg accaagcgga tccacaacac gaccaatcag actttcacct
    12421 acagggactt ccatgatttt ccctgtacgg cggattgtat cgccttcacg gatatctgta
    12481 aagtcaccta ggatgataat accaacgtct gttgactcca agttttgagc cataccataa
    12541 gagccgtttt caaaattcaa caactctcca ctcatgacat tttcaaggcc atgagcacgc
    12601 gcgataccgt ccccgatata ggttacaaca cctgtttcag tcacatcaaa attgggtttg
    12661 aaattttcaa tttgttgctt aattaaagcg ctgatttctt gtgcgttaat tgccaaaaga
    12721 acaccacttt ctatttcaaa ttttctttaa caactttaag ttgttgttta atactcacat
    12781 caattgtctt gtgattggca aaaatgacaa aaccaccaat gagactttca tcgatttgtt
    12841 cttttacact ccttactttc agagacattt ttttctcaat caaagggagc aaacgagtct
    12901 tctgttcatc agttagagga tgagcagacg taatcgtcac ttcaaatcga tttgtttctt
    12961 tttcaagtcg gttcaagcaa tctacaagca catcataaaa aagatttgct ctgtgattgt
    13021 aggccagaac ctggataaag ttttgtaata aaggcgacac agaatcttgg aaaaaagcaa
    13081 ttgttttttc cttatccgac tcgtctactg ccacttgttt taaaaaagaa ggcagacctg
    13141 ttttttcaac aacttgcttg atttgagtca agtctgaaaa gatacggtct tcttctcctt
    13201 tttcaagtac caattggaca aaaggcatgc tgtatttttc aattaccttt actgttttct
    13261 tgtccattaa gcttctccta gctgatcgat atactgatca atgagtgctt tatgggcatg
    13321 actgtcaagg ttttgtgaga tgattttacc agctaagctg atggtcaaat ctgcgacctc
    13381 acccttaaca ctctgtaaag cttctacttt attttgagca atttcttgat tggctttttc
    13441 ttttaagtgt cctgcttcta gtttagcatc tgctaagata ttagccttac tttgctcagc
    13501 tgtttccttt gcattttcaa tgattgtctt agcttcttta cggctaccag ccaattcatc
    13561 ttcgcgtttt tgagccaata cttctgcttt ttgacgggct tcttcagctc tatcaatatc
    13621 tgaagcaatt ttttcagctc tttcttcgaa aatgcctgta atattagacc atgcaaattt
    13681 tttaatcaag actagcaaaa gaataaaaga gccagtgatt aaaataaaat taccaattaa
    13741 ttcacctact gttacgtgca tcagttactc ctttctattc ctcttcaata ttaatcttat
    13801 tccctaaata cacagatgtc aaaagagtaa aaacataagc ttggatgcag gaaataaaga
    13861 cagaaaatgc agtccaagcc aaattagctc caaaggctac tggataccaa taaatagctt
    13921 ggtgggaaag aagaagtaac aaactcgtca tgacctctcc tgcaaagata ttcccaaaaa
    13981 tccgcaaagc caaagataag aagtttgtaa attcttccaa gatattcatc ggtatgacaa
    14041 aaacaggact cataaaagat tttatacttt ttttaaatcc acgacgacga acgctttcta
    14101 tatgtgtcaa caaaatgaca agaaaagata aggttaagtc atactgtaaa ttagcggttg
    14161 gcgaactcca ccagttagtc ccatcgatcg tttgaagctt tgtcattaag ccaaggttat
    14221 tggcaatcac catgaaaagg aataaacaaa ggaaaaagag tgagtaatct ttcatgtagc
    14281 gcgaaccaat gttaggttct gtaaatccaa taacaaagtc atagacatac tcaagtatat
    14341 tttgctttcc tttgggtttc aaggtcatat tgcggcttgc ccaataaata aagacaaaaa
    14401 taactcccac aatcaacaaa gtcatggcta acatagtcag attgaagata acaggaccaa
    14461 tagagatgat tggattaata ctttcttcca tctaaaatcc tcccttttct tctcgttcca
    14521 tgtatttatt tgataataaa tgagaagaca agagttacaa agaaagttcc ttcaataaag
    14581 gcaacaccta aaaacatcaa actacgaaac tcagaaagca tatctggttg gcgtgctact
    14641 gatttaaaca gtccattcat caataaacct tcaccgacag atacgcccat acaggcaata
    14701 cataagccta aaaatgttaa attcataaca aattctcctt taaaataaaa tttacttact
    14761 tagtgtagtc ctaaaattta aatttgtcaa cttttaaccg ctattggaag cgtttaataa
    14821 aaattatttc cattt
//
LOCUS       DAVLYR010000035        14536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLYR010000035 DAVLYR010000000
VERSION     DAVLYR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.035"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..954)
                     /locus_tag="VYO63_000848"
                     /pseudo
     CDS             complement(1..954)
                     /locus_tag="VYO63_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2057..3316
                     /locus_tag="VYO63_000849"
     CDS             2057..3316
                     /locus_tag="VYO63_000849"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1671802.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            3332..3883
                     /locus_tag="VYO63_000850"
     CDS             3332..3883
                     /locus_tag="VYO63_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1671803.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            3876..5153
                     /locus_tag="VYO63_000851"
     CDS             3876..5153
                     /locus_tag="VYO63_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1671804.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            5169..6029
                     /locus_tag="VYO63_000852"
     CDS             5169..6029
                     /locus_tag="VYO63_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1671805.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVIGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            6252..6362
                     /locus_tag="VYO63_000853"
                     /pseudo
     CDS             6252..6362
                     /locus_tag="VYO63_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
     gene            6408..7212
                     /locus_tag="VYO63_000854"
     CDS             join(6408..6776,6778..7212)
                     /locus_tag="VYO63_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1671806.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLLRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKAYNHA
                     LSKDRSKVENIFAKVKTFKMISTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            7404..9695
                     /gene="pcrA"
                     /locus_tag="VYO63_000855"
     CDS             7404..9695
                     /gene="pcrA"
                     /locus_tag="VYO63_000855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1671807.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            9738..10418
                     /gene="radC"
                     /locus_tag="VYO63_000856"
     CDS             9738..10418
                     /gene="radC"
                     /locus_tag="VYO63_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1671808.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(10415..11104)
                     /locus_tag="VYO63_000857"
     CDS             complement(10415..11104)
                     /locus_tag="VYO63_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1671809.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDSGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(11122..11763)
                     /locus_tag="VYO63_000858"
     CDS             complement(11122..11763)
                     /locus_tag="VYO63_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1671810.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(11846..12061)
                     /locus_tag="VYO63_000859"
     CDS             complement(11846..12061)
                     /locus_tag="VYO63_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1671811.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(12069..12416)
                     /locus_tag="VYO63_000860"
     CDS             complement(12069..12416)
                     /locus_tag="VYO63_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1671812.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(12421..13536)
                     /locus_tag="VYO63_000861"
     CDS             complement(12421..13536)
                     /locus_tag="VYO63_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1671813.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(13546..14505)
                     /locus_tag="VYO63_000862"
     CDS             complement(13546..14505)
                     /locus_tag="VYO63_000862"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1671814.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGAPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
BASE COUNT         4335 a         2627 c         3210 g         4364 t
ORIGIN      
        1 ttggttaaat gcatacgaaa agtaggacga taaaaacgtt tatcactcag tttacggcta
       61 tcctgttgaa tgagtttcca gtagcgcttg atagccttgt attcatggga ttttcgatga
      121 aactgattca tgatttggac acgcacacga ctcatagcac ggctaagatg ttgtacaatg
      181 tgaaagcgat caagaacgat tttagcattc gggagtgaaa cagtctggga gactgtttca
      241 gcctgagcct agaaatttga aagcgaagct gtttagccaa gccatagtaa gggctaaaca
      301 tatccatggt aatgattttg acgcgacatc ggacggctct atcatattta agaaagtgat
      361 ctcggatgac agcttgtgtt ctgccttcaa gaacagtgat gatattgaga ttgttaaaat
      421 cttgcgcaat aaagctcatc tccatctccc gattgaaaca gtcactcccc ggactgtttc
      481 aacgtcccag gacataatct caggaagaca agaaaaatca tgtttaaagt gaaaatcatt
      541 gagcttacga ataacagttg aagttgaaat ggccagctga tgagcaatat cggtcataga
      601 aatcttctca atcaactttt gcgcaatttt ttggttgata atacgaggaa tttggtgatt
      661 tttcttgacg atagaagttt cagcgaccat catttttgaa cagtgatagc acttgaatcg
      721 acgctttcta aggagaattc tagtaggcat accagttgtc tcaaggtaag gaatcttaga
      781 cggtttttga aagtcatatt tcttcaattg gtttccgcac tcagggcaag atggtgcgtc
      841 gtagtccagt ttggcgatga tttccttgtg tgtatcttta ttgatgatgt ctaaaatctg
      901 gatattaggg tctttaatgt ctagtaattt tgtgataaaa tgtaattgtc ccatatgaat
      961 ctttctaatg agttgttttg tcgcttttca ttatagatct tatgggattt tgtggtacga
     1021 caggcatgta tagcaaactg aatctggaat agcacagcat atcttctaaa atattgttat
     1081 aaattgattt gaatttcaaa attaaattgt ttgattctta tttcaatttg ttatagtata
     1141 tctgatgtca aagttctcgg cgaatcaaat agcgattccc aagcctgact atcgtgaggt
     1201 agcggattaa aatggtctgg ggatagaccg ttttaagtct gacgctggaa ataagaattg
     1261 tcagaagaag ggatagcgaa atcgtggctc tacgaacagg aacgtgataa taaggcgtat
     1321 atagcggata agagggcctc aaactctaaa gtccaaaaag gtagtcgtaa cctatatgcg
     1381 taaatcacga gagtaattga attcgtacta agattttcta ttttcactgt aaccttttaa
     1441 cgtcctcata tcttgtatac acgaggaaag atgtacgact tatcccgtga ggtctatcac
     1501 tataaagaga aaacgacaga tagaagtgat cctgagtcac ggttatcttt aaaatagatt
     1561 gtctgatagg atgctagcct gaaaacgtct tgttcatgaa atataggacg gtatgtataa
     1621 aacgcttctg tgaactgaga gaagggggag aagttcttgc taaaatttag ttgaacagcc
     1681 gtattccgat atttagataa gagatctagt cttagctcct actcggtttc aggggataaa
     1741 aaaggggcaa tagcgattcg agaaagatta tactcttcga aaatctcttc aaatcacgtc
     1801 aatatcgcct tgccgtacgt gtaggatact gactacgtca gttccatcta caacctcaaa
     1861 acagtgtttt gagcaacctg cggctagttt cctagtgtga tctttgattt ttattgagta
     1921 ttagtaattc agttactaac tcgtcaactc tgatttatcc aataaaattg aaaaggatgg
     1981 aaaaaaggat aaatttatga tatactttat tttgaagacc ttattagaaa tcttgaaaga
     2041 gtattgaaaa cttagaatga gaaaaattgt tatcaatggt ggattaccac tgcaaggtga
     2101 aattactatt agtggtgcta aaaatagtgt tgtggcctta attccagcta ttatattggc
     2161 tgatgatgtg gtgactttgg attgtgttcc agatatttcg gatgtagcca gtcttgtcga
     2221 aatcatggaa ttgatgggag ctactgttaa gcgttatgac gatgtcttgg agattgatcc
     2281 aagaggtgtt caaaatattc caatgcctta tggtaaaatt aacagtcttc gtgcatctta
     2341 ttatttttat gggagcctct taggccgttt tggtgaagcg acagttggtc taccgggagg
     2401 atgtgatctt ggtcctcgtc cgattgactt acaccttaag gcgtttgaag ctatgggtgc
     2461 cactgctagc tacgagggag ataacatgaa gttatctgct aaagatacag gacttcatgg
     2521 tgcaagtatt tacatggata cggttagtgt gggagcaacg attaatacga tgattgctgc
     2581 ggttaaagca aatggtcgta ctattattga aaatgcagcc cgtgaacctg agattattga
     2641 tgtagctact ctcttgaata atatgggtgc ccatatccgt ggggcaggaa ctaatatcat
     2701 cattattgat ggtgttgaaa gattacatgg gacacgtcat caggtgattc cagaccgcat
     2761 tgaagctgga acatatatat ctttagctgc tgcagttggt aaaggaattc gtataaataa
     2821 tgttctttac gaacacctgg aagggtttat tgctaagttg gaagaaatgg gagtgagaat
     2881 gactgtatct gaagacagca tttttgtcga ggaacagtct aatttgaaag caatcaatat
     2941 taagacagct ccttacccag gctttgcaac tgatttgcaa caaccgctta cccctctttt
     3001 actaagagcg aatggtcgtg gtacaattgt cgatacgatt tacgaaaaac gtgtaaatca
     3061 tgtttttgaa ctagcaaaga tggatgcgga tatttcgaca acaaatggtc atattttgta
     3121 cacgggtgga cgtgatttac gtggggccag tgttaaagcg accgacttaa gagctggggc
     3181 tgcactagtc attgctgggc ttatggctga aggtaaaact gaaattacca atatcgagtt
     3241 tatcttacgt ggttattctg atattatcga aaaattacgt aatttaggag cggatattag
     3301 acttgttgag gattaaaccg tagaggtgtt tatgaatatt tggaccaaat tagcaatgtt
     3361 ttcttttttt gaaacggatc gcttgtattt gcgtcctttc ttttttagtg atagtcagga
     3421 cttccgcgag atagcttcaa atccagaaaa tcttcaattt attttcccaa cgcaggcaag
     3481 tctggaagaa agtcaatatg cactggccaa ttactttatg aagtcccctt tgggagtgtg
     3541 ggcaatttgt gaccagaaaa atcaacaaat gattggttct attaaatttg agaagttaga
     3601 tgaaatcaaa aaagaagctg agcttggcta ttttttgaga aaagatgctt ggtcgcaagg
     3661 atttatgaca gaggttgtta gaaaaatttg tcagctttct tttgaggaat ttggcttaaa
     3721 acaattatct atcattaccc accttgaaaa tgaagctagc caaagagttg ctcttaagtc
     3781 tggatttagt ttgttccgtc agtttaaggg aagtgatcgt tacacaagaa aaatgcggga
     3841 ttatcttgaa tttcggtatg taaaaggaga gttcaatgag taagcatcag gaaattctaa
     3901 gctatttgga ggaattacca gtaggtaaaa gggtcagtgt tcgtagcatt tcgaatcatc
     3961 taggagttag tgatggaaca gcctatcggg ctattaaaga agctgaaaac cgtggaattg
     4021 tggagacccg tcctagaagt ggaacaattc gtgttaaatc ccagaaagtt gctatagaga
     4081 gattaacgtt tgctgaaatt gcagaagtga cttcttctga ggttctggct gggcaagaag
     4141 gtttagagag agaatttagt aagttttcaa ttggtgccat gactgaacaa aatatcttgt
     4201 cttaccttca tgatgggggg ctcttgattg tcggagaccg aacccgtatt cagttgctag
     4261 ccttggaaaa tgaaaatgca gttctggtta cagggggatt tcaggttcat gatgatgtgc
     4321 ttaaactggc caatcaaaaa gggattcctg ttctaagaag taagcatgat acctttaccg
     4381 tcgcgaccat gatcaataaa gccttgtcaa atgtccaaat caagactgat attctgacag
     4441 ttgagaaact ttatcgccct agtcatgagt atggttttct gagagagaca gatacagtta
     4501 aagattattt ggacttggtt cgtaagaatc gtagcagccg tttccctgtt atcaatcaac
     4561 atcaggtcgt tgttggtgtt gtaaccatga gagacgctgg tgataaatca ccaagcacga
     4621 caattgataa ggttatgtct cgtagtctat ttttggttgg attatcgaca aatattgcca
     4681 atgtgagtca acggatgatc gcagaagact ttgaaatggt accagttgtt cgaagcaatc
     4741 aaactttgct tggcgttgtg acgcgacgag atgtcatgga gaagatgagc cgttcccaag
     4801 tttcggctct accaactttt tctgagcaga ttggacaaaa gctctcttat caccatgatg
     4861 aagtagtcat tacagtggaa ccctttatgc tagaaaaaaa tggagttttg gctaatggtg
     4921 tattggcaga aattctgacc cacatgaccc aagatttagt tgttaatagt ggtcgcaatc
     4981 tcattatcga gcagatgctg atctactttt tgcaggctgt tcagatagat gatatattgc
     5041 gcattcaggc acggattatt catcatacga gacggtcagc tataattgat tacgatattt
     5101 atcatggtca ccagattgtt tcaaaagcaa atgtgactgt taaaattaat tagaaactag
     5161 gagaaaagat gataacatta aaatcagctc gtgaaatcga agctatggac aaggctggtg
     5221 attttctagc aagtattcat ataggcttac gtgatttgat taagccaggc gtagatatgt
     5281 gggaagttga agaatatgtc cgccgtcgtt gtaaagaaga aaatttcctt ccacttcaga
     5341 ttggggttga cggtgccatg atggactatc cttatgctac ctgttgctct cttaacgatg
     5401 aagtggctca cgctttccct cgtcattata tcttgaaaga tggtgatttg ctcaaagttg
     5461 atatggtttt gggaggtccc attgctaaat ctgacctgaa tgtctcaaaa ttaaacttca
     5521 acaatgttga acaaatgaaa aaatacactc agagctattc tggtggttta gcagactcat
     5581 gttgggctta tgctgttggt acaccgtccg aagaagtcaa aaacttgatg gatgtaacca
     5641 aagaagctat gtacaagggt attgagcaag ctgttattgg aaatcgtatc ggtgatatcg
     5701 gtgcggctat tcaagaatac gctgaaagtc gtggttacgg tgtagtgcgt gatttggttg
     5761 gtcatggtgt tggcccaact atgcacgaag aaccaatggt tcctaactat ggtattgcag
     5821 gtcgtggact ccgtcttcgt gaaggaatgg tcttaaccat tgaaccaatg atcaatacag
     5881 gcgattggga aattgataca gatatgaaaa ctggttgggc gcataagacc attgacggtg
     5941 gattgtcatg tcagtatgaa caccaatttg tcattacgaa agatggacct gttatcttga
     6001 ctagccaagg tgaagaagga acttattaat aaaaagtgaa aagactactg gaagtttatt
     6061 atgaaaaaat ccagtagatc ttttcataat aaaacgcatt gtatcaagtg ttaggggctg
     6121 atatcatgcg tttttctgct tttaagattt tttccaactc tgtttgtaag cgcatcataa
     6181 caaagggtct aggattcagg gctctcctcc tatatactat tagtaaagta aaactaaggg
     6241 aggatatttt agtgtcgcag tctattgttc ctgtagagat tccacaatat tgtcgttttg
     6301 attctaaaaa gagaaatgga attctgttta atgttcgtat tgccaatctt aaatttacct
     6361 tttttagatt agacttcctg cgaaacaaaa tatggtatag tagttccatg aattatgaag
     6421 caagtaaaca actaactgat gcacgattta agcgtcttgt tggtgttcag cgtaccactt
     6481 ttgaagagat gttagctgta ttaaaaacag cttatcaact taaacacgca aaaggtggac
     6541 gaaaacctaa attaagccta gaagaccttc ttatggccac tcttcaatat gtgcgagaat
     6601 atcgaactta tgaacaaatt gcggctgatt ttggtattca cgaaagcaac ttactccgtc
     6661 ggagccaatg ggttgaagta actcttgttc aaagtggttt tacgatttca agaactcctc
     6721 tcagttctga ggacacggta atgattgatg cgacggaagt aaaaatcaat cgccctaaaa
     6781 aaagaattag cgaatgattc tggtaaaaag aaatgccacg ctatgaaggc tcaagcgatt
     6841 gtcacaagtc aagggagaat tgtttctttg gatatcgctg tgaactattg tcatgatatg
     6901 aagttgttca aaatgagtcg cagaaatatc ggacaagctg gaaaaatctt ggctgacagt
     6961 ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa
     7021 ctcaagccgc taacagttga agataaagcc tataaccatg cgctatccaa ggatagaagc
     7081 aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tgatttcaac aacttatcga
     7141 aatcatcgta aacgtttcgg attacgaatg aatttgagtg ctggtattat caatcatgaa
     7201 ctaggattct agttttgcag gaagtctatt gaggtattga gctagtttat gaaaaaattg
     7261 ggtgaaaaag tcgagtgttt tagaaaccca cagtgtagta ttctagtttc aatccactat
     7321 attttgctac tccccgtaaa gtttctattt tccctgattt ctgatataat agaaatattg
     7381 acttcaagag taaggaagag aagatgaacg cattattaaa tggaatgaat gaccgtcagg
     7441 ctgaggcggt gcaaacgaca gaaggtccct tgctaatcat ggcaggggct ggttctggaa
     7501 agactcgtgt tttgacccac cgtatcgctt atttgattga tgaaaagctg gtcaatcctt
     7561 ggaatatctt ggccattacc tttaccaaca aggctgcgcg tgagatgaaa gagcgtgctt
     7621 atagcctcaa tccagcgact caggactgtc tgattgcgac cttccactcc atgtgtgtgc
     7681 gtattttgcg tcgcgatgcg gaccatattg gctacaatcg taattttaca attgtggatc
     7741 ctggtgaaca gcgaacgctc atgaaacgta ttctcaaaca gttgaacttg gatcctaaaa
     7801 aatggaatga acgaactatt ttggggacca tttccaatgc taagaatgat ttgattgatg
     7861 atgttgctta tgctgcccaa gctggcgata tgtatacgca aattgtggcc cagtgttata
     7921 cagcctatca aaaagaactt cgtcagtctg aatccgttga ctttgatgat ttgattatgc
     7981 tgaccttgcg tctctttgat caaaatcctg atgttttgac ctactaccag caaaaattcc
     8041 aatacatcca cgttgatgag taccaagata ccaaccacgc tcagtaccaa ttggtcaaac
     8101 tcttggcttc ccgttttaaa aatatctgtg tggttgggga tgcggaccag tctatctacg
     8161 gttggcgtgg tgctgatatg cagaatatct tggactttga aaaggattac cccaaagcca
     8221 aggttgtttt gttggaggaa aattaccgct caaccaaaac cattctccaa gcggccaacg
     8281 aggttattaa aaacaataaa aatcgccgtc ctaaaaatct ctggactcaa aacgctgatg
     8341 gggagcaaat cgtttactat cgtgccgatg atgagctgga tgaggctgta tttgtagcca
     8401 gaaccatcga tgaacttagt cgcagtcaaa acttccttca taaggatttt gcagttctct
     8461 atcggactaa tgcccagtcc cgtacaattg aggaagccct gctcaagtct aacattcctt
     8521 ataccatggt tggcggaacc aaattctaca gccgtaagga aattcgcgat attattgctt
     8581 atctcaacct tattgctaat ttgagtgaca atattagttt tgagcgtatt atcaacgagc
     8641 ctaaacgtgg aattggtcca ggtacagttg agaaaatccg tgattttgca aatttgcaaa
     8701 atatgtctat gctggatgct tctgctaata ttatgttgtc tggtatcaag ggtaaggcag
     8761 cccaatctat ctgggatttt gccaatatga tgcttgattt gcgggagcag ctagaccact
     8821 taagcattac agagttggtt gagtccgtcc tagaaaaaac aggttatgtc gatattctta
     8881 acgcccaagc gactctagaa agcaaggcac gggttgaaaa tatcgaagag tttctttctg
     8941 ttacgaagaa ctttgatgac accacggatg tgacagaaga ggaaactggt ctggacaaac
     9001 tgagtcgttt cttaaatgac ttggctttga ttgccgacac agattcaggt agtcaggaga
     9061 catcagaagt gaccttgatg accctgcatg ctgccaaagg tctcgaattt ccagttgtct
     9121 ttttgattgg gatggaagaa aatgtctttc cacttagtcg tgcgactgaa gatccagatg
     9181 aattagaaga agagcgccgt ctagcctatg taggtatcac gcgtgcagag aaaattctct
     9241 atctgaccaa tgccaactca cgcttgcttt ttggtcgtac caattataac cgtccgactc
     9301 gttttattaa cgaaatcagt tcagacttgc ttgagtatca aggtctggct cgtcctgcaa
     9361 atacaagctt taaggcatca tatagcagtg gtagtatttc ctttggtcaa ggtatgagtt
     9421 tggctcaggc tcttcaagac cgtaaacgcg gtgctgcccc aaaatcaatc cagtcaagcg
     9481 gtcttccatt tggtcaattt acagctggcg caaaatcagc atctagcgag gcaaattggt
     9541 ccattggtga cattgctctc cacaagaaat ggggagaggg aactgttctg gaagtttcag
     9601 gtagcggtgc taggcaggaa ttgaaaatca atttcccaga agtaggtttg aaaaaacttt
     9661 tagccagtgt ggctccaatt gagaaaaaaa tctaattttc catccttctc acgaataata
     9721 aagtgaggag gatttttatg tacagtattt cattccaaga agattcacta ttaccaagag
     9781 aaaggctggc caaggaagga gttgaagcgc ttagtaacca agagttgcta gctattttac
     9841 tcaggacagg aacacgtcaa gctagcgttt ttgaaattgc ccaaaaagtc ttgaacaatc
     9901 tttcaagcct aacggatttg aaaaaaatga ccctgcagga attgcagagt ttgtctggta
     9961 ttgggcgtgt taaggccata gaattacaag ctatgattga actggggcat cgtattcaca
    10021 aacacgagac tcttgaaatg gaaagtattc tcagcagtca aaagttggcc aagaagatgc
    10081 agcaggaatt aggggataaa aaacaagagc acctggtggc actctatctc aatactcaaa
    10141 atcaaatcat ccatcagcag accattttta tcggttctgt aactcgtagt atcgctgaac
    10201 cgcgagagat tctgcactat gcaatcaagc atatggcgac ttctcttatc ttggtccaca
    10261 atcatccttc aggagcggta gcgcctagcc aaaatgatga tcatgtcact aaacttgtta
    10321 aagaagcctg cgaattgatg gggattgttc tcttggacca tttgattgtc tctcattcta
    10381 attactttag ttatcgtgaa aagacagatt taatctaaag ttcattaacg acatagtcaa
    10441 agagtttttt atctttggga cgattttcaa aaagaagttc tggatgccat tggacaccga
    10501 gaaaggcgac atcatccgta ctcatgacag cctcaatgat accatcttta ggatcatgag
    10561 ccacaacttt taaatttggt gctaagtcct tgatgctctg gtggtggaag gagttgatat
    10621 gagagatttc tccatagatt tcttggagaa cggtatctgg ttctgttacc aagcgttgag
    10681 ttgtgtactc aacagaagaa tcctgccaat ggtcttcgat atcttggtac aaagttccac
    10741 ccatggcaac gttaaagagt tgggtaccac ggcagacaga gaaaatgggc tttttctgtt
    10801 taatagcttc cttgatgagg gtcagttcga agatatctct ttgaaggtga tagtcatcgc
    10861 tatcaatggt tttgggttcg ccataaaatt ttggatcgac attttgccca cctgtcaaga
    10921 tgagcttgtc aatcaaactg atatagtggc aggccatttc ttgatcacca atcggtagga
    10981 tgatgggaat ccctccagaa tctttaacgc cttcaacaaa gccttttgct gcgtagctca
    11041 tcatgatgtc atcatctgga tgagcttttt cgtttcctgt aatcccaata actggttttt
    11101 tcataaaatg attttcgctt tctaatcctc ttttcgcatg aagtagagga gggtttggag
    11161 ttcacttgtc aaatcgacat actgaacgac cacgtctttt ggtaaatgca gatggactgg
    11221 tgaaaaactg agaattcctt tcacaccagc atcaaccaag agattagcaa cctcttgtga
    11281 cttgacgctg ggaacagtta ggatagcagt cttcacatca gcatccttga ttttatcctt
    11341 gatctgagaa atcccgtaaa tgggaatccc gtcaggagtt tgggtaccga cttcaggatg
    11401 gtcgtctagg tcaaaggcca tgataatctt catcttgtta cgttcgtgga agcggtagtg
    11461 gagaagggca tggcccatat ttccaatacc aaccagcatg acattggtaa tagagttgtc
    11521 attgagcaaa tcggcaaaaa atgtcattag ttttttgaca tcatagccaa aaccacgacg
    11581 accaagttca ccaaaatagg aaaaatcacg acgtacggtc gctgaatcaa taccgatagc
    11641 ctctgcaatt tgcttagagt tggcacgttc aatcttttct gcatgaaatc tcttaaaaat
    11701 tcgatagtag agagagagtc tttttgctgt agcttttgga atagcaaact gtttatcttt
    11761 cacaaaatca caacctttct attcttctat tttatagaaa cattgtgaaa aaatcaacaa
    11821 aaataagaaa aaactaagaa aaatcttagt tttgatgtaa aaaatctgca tgagatagaa
    11881 aacggtagag gtctccgacc agaccctgat aaactttttg gcccctaaaa gtcagagaag
    11941 tcacataaag tgtatctggt aaggttacac atcctgacaa agtcaacatg agagcctcat
    12001 gatcctcata cttgagagta cgctctacat gatagcagtc cttataggtc agttcaaaca
    12061 ttttggctct atctttccga ttttgtaaag acaccacgtt ctaccaagct atccatgagg
    12121 aagtagaatt tttcctgatg aatatggtgg tcttctgatt tgaaaatatc aactagacga
    12181 aggccaaact tgtcagtgat attgatttta gcccctgtaa gttccttgtt aatgatgatt
    12241 ttgagttgga agccttcacc gctgtttggc actttttcca aaaggcgagt cagttcatag
    12301 ttaccaacct tagtttcaaa aaaggtgtta tctttgaggg tgaatttttt aacagaaggg
    12361 ctaagagtgt aatcgtaacg acaatttttt aactgaatga ttttttcaaa tgccatatgg
    12421 ctaacctccg ataatttctt ttaaggtttt tgcgagggtt tgtaggtctt caacggtatt
    12481 ttgtggcgac aaactgatgc gaagggattc cttcaagcgt tctgaatttg cgccatacat
    12541 ggcttcaaga acatggctgg attggacaac gcctgcagta caggctgagc cagtagagat
    12601 tgaaattcca gctaaatcta gccgaaggag taagaggtca tttttctgac caggaaatcc
    12661 aatattgaga acataaggga gatgatgttt tcctctattc aagtaatact gaatgccctc
    12721 cagctctgcc agaaaggcag tttctagatt ttgtacatgt tgaaaatgtt cttcttgttt
    12781 ttctaggtct tcttttaggg ctgcaaccat gcctacgata gcaggcagat tttcagttcc
    12841 tgcacgtttt ttctgttcct ggtctccgcc atgtagatag gaatcaaagt ccatgctaga
    12901 tgcgtagaga aaaccgattc ccttaggacc atggaatttg tgggcagaag cagtgagaaa
    12961 atcaatgccc aattcttctg aatgaattgg gattttacca atagcctgaa ctgcatcaac
    13021 atgataggca gcagggtgtt gcttgagtat ttggccaatt tcagcgatgg gcagtaggtt
    13081 tcctgtctca ttattggcaa acatggtaga aaccaaaatc gtatcgtcac gtaaagcctt
    13141 ttgaatttgc tgggctgtga tttcttgatt ttctggctgg ataatggttg cttcaaaccc
    13201 aaagtgttga accaagtaat caattgtttc aaggacagca tggtgctcga tggcagttgt
    13261 gatgatatgt tttccttgtt cttggtgacg aagacagtag ccaatgatgg tagtattatt
    13321 gccttcagtc ccaccagaag tgaaaaagat atgttgaggt tttgtcctta gtaactgggc
    13381 tagttcctga cgggcttctc gcaagagttt gccagcttga cgaccatgac catgaatact
    13441 agaaggattt ccgtgggttt cttgcataac cttggtcata gctgaaatag caactgctga
    13501 cataggagtc gttgcagcat tgtccaaata aatcaaagaa tcaccttatt tctttttatt
    13561 gtaggcaaag agtgggctga ctggttttct ttcgtgaata cggacgatag catcaccaat
    13621 taactcacta gcagtgatgt agcatacatt tttaggagtt ttttcttttg ttgctactga
    13681 atcagtcaca agaatttctt taatattagt attgtcaaga agctcagcag ctccctcgac
    13741 gaagagaccg tggctagaaa cagcataaat ttctgtagct ccttcacgtt caacgatttt
    13801 agaagcttca gagaaggtac gtcctgtatt taaaatatca tcaatcaaga tagctttctt
    13861 accttcaaca tcaccaataa tataaccttc gttacgagtt gcatcgtctt gagggtagtc
    13921 gataatggcg ataggagcat caagatattc agccaggcta cgcgcacgtt tgacacctga
    13981 atttttaggg ctaacgacaa caacatctga accaagcaat cctttatcgc agtaatgttt
    14041 tgcgaatagg ggaacagtga aaagattatc cactggaata tcaaagaaac cttgaacctg
    14101 aacggcatgc aaatcaagag tcaggatacg atcaactcca gccttaacca gcatattggc
    14161 aactagtttt gctgtaagtg gctcacgagg acaagcaatg cggtcttgac gtgcatagcc
    14221 aaaatatgga aggacaacgt tgatactgtg ggcacttgca cgcacacaag catcgaccat
    14281 gattaacaat tccattaggt ggttgttgac agggaaactt gttgattgga tgatgtaaac
    14341 atcataacca cggacacttt cttcgatatt tacttggatt tctccgtctg aaaattgacg
    14401 tgatgatagt tttccaagtg gggcaccaac agcttgggca attttttgtg caatctcttg
    14461 gttagagttg agtgcgaaaa gtttcatgtt ttttctatct gacattatag accgtcctct
    14521 gtaaacttta taaatc
//
LOCUS       DAVLYR010000036        14021 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLYR010000036 DAVLYR010000000
VERSION     DAVLYR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.036"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            357..1520
                     /locus_tag="VYO63_000863"
     CDS             357..1520
                     /locus_tag="VYO63_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1671815.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1517..2194
                     /locus_tag="VYO63_000864"
     CDS             1517..2194
                     /locus_tag="VYO63_000864"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1671816.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2184..3038
                     /locus_tag="VYO63_000865"
     CDS             2184..3038
                     /locus_tag="VYO63_000865"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1671817.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEAHLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3057..4124
                     /locus_tag="VYO63_000866"
     CDS             3057..4124
                     /locus_tag="VYO63_000866"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1671818.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4134..5300
                     /gene="aroC"
                     /locus_tag="VYO63_000867"
     CDS             4134..5300
                     /gene="aroC"
                     /locus_tag="VYO63_000867"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1671819.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5310..6413
                     /locus_tag="VYO63_000868"
     CDS             5310..6413
                     /locus_tag="VYO63_000868"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1671820.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLESIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            6424..6762
                     /locus_tag="VYO63_000869"
     CDS             6424..6762
                     /locus_tag="VYO63_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1671821.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6854..8137
                     /gene="aroA"
                     /locus_tag="VYO63_000870"
     CDS             6854..8137
                     /gene="aroA"
                     /locus_tag="VYO63_000870"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1671822.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSASFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8130..8606
                     /locus_tag="VYO63_000871"
     CDS             8130..8606
                     /locus_tag="VYO63_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1671823.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8603..9451
                     /gene="pheA"
                     /locus_tag="VYO63_000872"
     CDS             8603..9451
                     /gene="pheA"
                     /locus_tag="VYO63_000872"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1671824.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEGVTIPLQSQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9448..10722
                     /gene="psr"
                     /locus_tag="VYO63_000873"
     CDS             9448..10722
                     /gene="psr"
                     /locus_tag="VYO63_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1671825.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11001..11846
                     /locus_tag="VYO63_000874"
     CDS             11001..11846
                     /locus_tag="VYO63_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1671826.1"
                     /translation="MNNMTDLKAIQARSLEMADYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11836..12939
                     /locus_tag="VYO63_000875"
     CDS             11836..12939
                     /locus_tag="VYO63_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1671827.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12941..13927
                     /locus_tag="VYO63_000876"
     CDS             12941..13927
                     /locus_tag="VYO63_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1671828.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13943..>14021
                     /locus_tag="VYO63_000877"
     CDS             13943..>14021
                     /locus_tag="VYO63_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HEV1671829.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVY"
BASE COUNT         4134 a         2595 c         3281 g         4011 t
ORIGIN      
        1 aacaaaaggc ttttctataa aatcaaattt tccagatatc tactgaagct gatttatttc
       61 ttcatctttc ttctactacc tttgtcacta aatttgacag acacatattg ctgatacaac
      121 ctctctacct ataatctcac ggttaacctg aggctagttt catagtttgc tatttgattt
      181 tcgttgataa aaagaaatgc tcctccaaga cggaagagca ttttttactg tgattcttac
      241 acatttactt cctagttttg caggaagtct agtataccat tttttaaaag aagagtaaaa
      301 aagcaagggt agtttctttt ttgagaattg tctaaaagtt tgatataatg gtagttatga
      361 atagaataag agtcagcaaa agggttgaaa agaagcttgc taaggggcta gttttactag
      421 aagccagtga tcttgagaat gtcaatctta aggatcagga agtagaggtg cagggtcagg
      481 aaggaaactt tcttgggact gcctaccttt ctcagcaaaa caagggcttg ggctggttta
      541 tcagcaaaga caaggtggcc ttcaatcaag ctttctttga aacgttgttt agaaaagcca
      601 aagaaaagag aaacgcctac tatcaagatg atttgacaac tgcctttcgt ctctttaatc
      661 aagagggaga tggctttggg ggtctgacag tggaccttta tggcgactac gccgtctttt
      721 cttggtataa ctcttatgtt tatcagattc gtcagactat atcagaagcc tttagacagg
      781 ttttccctga ggttttagga gcttatgaga aaatccgctt taagggtttg gactatgaat
      841 ctgcccatgt ttatggtcaa gaagcacctg actttttcac tgttttagaa aatggtgtcc
      901 tgtatcaagt ctttatgaat gatggcttga tgacaggaat tttcctagac cagcatgagg
      961 ttcgcggtag tttagttgac ggcttggcta tgggtaaatc cttgctcaat atgttttcct
     1021 acacagcggc tttttcagta gctgcggcca tgggaggagc tagccagaca acttctgttg
     1081 atctagccaa acgttcacga gaattgtctc aagcgcattt tcaggcaaat gggctcagca
     1141 cagacgagca tcgttttata gtcatggatg tctttgagta tttcaaatat gccaaacgca
     1201 aagacttgac ctacgatgtg attgtcctag atccgcctag ctttgctcgg aataaaaaac
     1261 aaactttctc tgtggccaag gattatcaca agttgatttc ccagagtctt gagattttaa
     1321 atccgggagg gattatcatt gccagtacca atgctgccaa tgtttcccgt cagaaattta
     1381 cagaacaaat tgataaaggt tttgcaggaa gaagttacca gattttaaac aaatatggtc
     1441 ttccagcaga ttttgcctat aataaaaaag atgaaagtag taattacctc aaggtgatta
     1501 gtatgaaggt tagtaaatga aattaatcgt ttcagtaatg ccaagaagtt tagaggaggc
     1561 tcaggctctg gatgccacga ggtacctgga tgccgacatc attgaatggc gtgccgacta
     1621 tctgcctaaa gaagcgattt tgcaggtagc tccagccatt tttgaaaaat tcgcaggccg
     1681 tgagttggtt ttcacgctac gaactcgctc cgaaggtgga gaaatcgacc tttctccaga
     1741 agagtatatc catctaatca aggaagttgc gcaactctat caaccagact atattgattt
     1801 tgagtactat agctacaagg atgtttttga ggaaatgctg gacttcccaa atcttgtttt
     1861 gagttaccat aatttccaag aaacacctga gaacatgatg gaaatcttgt cagagttgac
     1921 gatcctaaat ccaaaacttg ttaaggttgc ggtgatggct cacacggagc aggatgtctt
     1981 agacttgatg aactatacac gaggctttaa aaccctcaat cctgaacagg aatatgtgac
     2041 catttctatg ggtaaggtgg gcaaggtctc tcgtatcact gcggatgtga ctggttcgag
     2101 ttggtctttt gccagtctgg atgaggttag tgcccctggg cagatttctc tagctagcat
     2161 gaaaaaaatc agggagattt tggatgaagc ttgatggcta tacacgttta gctgcagttg
     2221 ttgccaatcc tattaagcat tctatttctc ccttcatcca caatagagcc tttgaggcga
     2281 cagctaccaa cggtgcttat gtggcttggg agattgaagc gagtgacttg gcagaaacag
     2341 tggccaatat tcgtcgctac cagatgtttg gcatcaatct gtccatgccc tataaggagc
     2401 aggtgattcc ttatttggat aagctgagcg atgaagcgca cttgattggt gcggttaata
     2461 cggttgtcaa tgagaatggc aatttaattg gatataatac agatggcaag ggatttttta
     2521 agtgcttgcc ttcttttaca atttcaggta aaaagatgac cctgctgggt gcaggtggtg
     2581 cggctaaatc aatcttggca caggctattt tggatggcgt cagtcagatt tcggtctttg
     2641 ttcgttccgt ttctatggaa aaaacaagac cttacctaga caagttacag gagcagacag
     2701 gttttaaagt ggatttgtgt gctttagaat atgtttctga actgcaagca aggattgccg
     2761 agtcggattt gctagttaat gccaccagtg tgggcatgga tggccaatcc tctcctgttc
     2821 ctgaaaacat agtcttacca gaaactctct tagttgcaga tatcatatac caaccctttg
     2881 aaacaccgtt tttgaaatgg gctagaagtc agggcaatcc agcagtcaat ggtctgggaa
     2941 tgttgctcta tcaagctgca gaagcttttc aactgtggac aggcaaggaa atgccgacag
     3001 aagagatttg gcagtcttta acagaaaaat accaataaag aaagggagac tctcctatga
     3061 aaatcagaat cgatattcct catcatcctt atgatattca gattgaaaaa ggttgtatgg
     3121 cccaggctgg tcagtggttg cgagaactct ggcaaccgca aaaggtagtc attgtgacag
     3181 ataaccatgt agcctctctc tatgcagaga aggtcaagct cagcctagaa gatgctggtt
     3241 ttcaggtagc tgtttttgat ttcttagaag gtgaagaaag aaagaattta actactgttc
     3301 agaaagtcta tgaatttcta gtcaagcaag gtctgactcg tagcgatgga atcgttgctc
     3361 ttggtggtgg cgttgttggg gacctggctg gatttgtagc ctctacctat atgcggggta
     3421 ttcactttgt tcagattccg actagtttga cagctcaggt tgattcttct atcggtggaa
     3481 agacaggcgt taacacacca tttgctaaaa atatggtggg gacctttgcc caaccagatg
     3541 gggttctgat tgatccactt gttcttgaaa ccctcggaaa aagagagttg attgaaggga
     3601 tgggtgaagt tatcaagtat ggcttgattg aggatccaga actgtgggct ctcttgacgg
     3661 gactgaatgg ttctgttgag agtattttgg aacatgcaga gaccttgatt gaacattctt
     3721 gtcaggtgaa gcgcaagatg gtggttgaag atgagttgga caatggtatt cgtctttacc
     3781 tcaattttgg ccacactatt ggccatgcca tcgaagcgac tgccggttat ggcaaggtca
     3841 tgcatggaga ggctgttgcc atgggaatgg tacagatttc caaggttgct gaggaaaaag
     3901 gcctcatgcc agctggcatt acccaatcta tcacagagat gtgtcagaaa ttcggcttgc
     3961 ctgttgacta tgaaaattgg aaagttgaca agctttatca ggctcttact catgacaaga
     4021 aagcgcgtgg taacaccttg aaattggtct tggtgccaga gcttggttca gcgaccattc
     4081 acccagtttc tctggaagag atgaaagact acttggtaaa ataaggagag tgtatgagat
     4141 atttaactgc aggagaatca cacggccccc gtctaacagc tattattgag ggaattccag
     4201 ctggacttcc attgacagct gaggatatca atgaggatct tagacgccgt cagggtggct
     4261 acggtcgtgg tggtcgtatg aagattgaga gtgaccaggt tgtctttact tcgggtgttc
     4321 gccacgggaa gacgacaggg gcgcctatta ctatggatgt catcaataag gaccaccaga
     4381 aatggctgga catcatgtct gcggaggaca ttgaagaccg ccttaaaagc aagcggaaaa
     4441 ttactcatcc tcgcccaggt catgccgatt tggttggggg gattaagtac cgttttgatg
     4501 atttgcgaaa ttctttggag cgttcatcag ctcgtgaaac caccatgcgg gtggcagttg
     4561 gtgcagtagc caaacgcctc ttggctgagc tggatatgga gattgccaac catgtcgtgg
     4621 tctttggtgg taaggaaata gatgttcctg aaaatctgac agtcgctgaa attaagcaaa
     4681 gagctgccca gtctgaagtt tctattgtca accaagaacg agaacaggaa atcaaggact
     4741 atattgacca aatcaaacgt gatggtgata ccatcggtgg ggttgtggag acagtcgtcg
     4801 gaggagttcc agttggtctt ggttcctatg tccaatggga tagaaaattg gatgcaagat
     4861 tggctcaagc tgttgtctct atcaatgcct ttaaaggggt ggaatttggt cttggctttg
     4921 aggctggtta tcgtaaaggc agccaagtta tggatgaaat tctctggtct aaagaagacg
     4981 gttatactcg ccgtaccaat aatctaggtg gttttgaagg tggtatgact aatgggcaac
     5041 ccatcgttgt tcgtggggtc atgaaaccca ttcctactct ttataaacct cttatgagtg
     5101 tggatatcga aacccacgaa ccttacaagg caaccgtgga gagaagtgat ccgactgctc
     5161 ttccagctgc aggaatggtc atggaagcag ttgtagcaac ggttctggcg caagagatcc
     5221 tcgaaaaatt ctcatcagat aatcttgagg aactaaaaga agcggtagcc aaacaccgag
     5281 actatacaaa gaactattaa ggagttccta tggcaaaaac aatctatatc gcaggtcttg
     5341 ggttgattgg agcctctatg gcacttggta tcaaacgcga tcatccagat tatgaaattt
     5401 taggttataa tcgtagtcaa gcttcgagag atatcgcctt gaaagaaggc atgattgacc
     5461 gtgcaacgga tgattttgct agttttgctc ctttggcaga tgtcattatc ctcagcttgc
     5521 caatcaaaca aactattgct ttcattaagg agttggccaa tttggatttg cgagaaggcg
     5581 ttattatttc agatgctggt tcgaccaagt caaccattgt ggatgcggcg gagcagtatt
     5641 tggctggcaa gtctgttcgc tttgtcgggg cccatcccat ggctggtagt cacaagacag
     5701 gggctgcttc ggcagatgtc aatctttttg aaaatgccta ttatatcttt acaccttcaa
     5761 gcctgacaag tcaggacacg cttaaggaaa tgaaggatct gctttcaggt cttcatgctc
     5821 gttttatcga gattgatgcc aaggagcatg atcgtgtcac ttctcagatt agccattttc
     5881 ctcatatttt ggcttctagt ctcatggagc agactgcggt ctatgctcaa gagcatgaga
     5941 tggcaaggcg ctttgcggca ggtggttttc gagatatgac ccgaattgcg gaaagcgagc
     6001 caggaatgtg gacctccatt ctcttgtcca atagcgagac cattctggat agaattgagg
     6061 atttcaagga acgtttggaa tcgattggtc aggccattag taagggagat gaagagcaaa
     6121 tttggaactt ttttaaccaa gcgcgtgaac aacgccagac catggaaatc cataagcgtg
     6181 gtggtgtgga tagctcttac gacctctatg ttgacgttcc tgatgaagaa gatgtcatcc
     6241 tgcggatttt ggaactgcta cgtgggactt ccttggttaa catccacatc aacgaggaaa
     6301 atcgtgagga tattcacgga atcctacaaa tttcatttaa aaatgctcaa gacttggaaa
     6361 gagccgagca tctcataaca aaaaataccg actatacagt cgtcattaaa taaggagaag
     6421 aacatgtcaa atatttacga tagtgcaaac gaactcagtc gcggtctacg cggattacca
     6481 gaatacaagg ctgttaaagc agctaaagat gcgattgcag cagatgctga ggcaagcaaa
     6541 atctttacag aatatcttgc ctttcaagag gaaattcaaa aactagcaca gacaggtcaa
     6601 atgccagatg cgtccttcca agctaagatg gaaggctttg gtaaacagat tcaaggaaat
     6661 agcctcttgt cagaattctt taccaagcaa caacaattgg ccatttacct ttctgacatt
     6721 gaaaaaattg ttttcgaacc agtttcagaa ttgctgaaat aagaaaagaa ctatcctaaa
     6781 gtcaggaatt tttaaggaat ttctggcttt ttatgataaa ataggtaaaa gtattgcaag
     6841 tatgaggtcc agtatgaaac taaaaacaaa tattcgccat ttacatggta gtatccgcgt
     6901 cccaggtgac aagtctatca gccaccgttc cattatcttt ggaagtttgg ctgagggtga
     6961 gaccaaggtt tatgatattc tgcgaggtga agacgttctt tcgaccatgc aggtttttcg
     7021 tgaccttggt gttgaaattg aggataaaga tggggttatt accattcaag gtgtaggcat
     7081 ggctggctta aaagcgccgc aaaatgccct taatatggga aattctggca cctcgattcg
     7141 cctgatttca ggtgtccttg ctggtgcaga tttcgaagta gagatgtttg gagatgatag
     7201 tctttccaaa cgtcctatgg accgtgtgac ccttccactg aaaaaaatgg gcgtcagcat
     7261 ctcagggcaa actgaacgag acttgcctcc ccttcgctta aaagggacga aaaacctaag
     7321 acctattcat tatgagttgc caattgcctc tgcccaagtc aagtcagcct tgatgtttgc
     7381 agccttacaa gctaaggggg agtcagttat tatcgaaaaa gagtgcaccc gtaatcatac
     7441 tgaagatatg ttgcaacaat ttggtggcca tttaagtgtg gatggcaaga aaatcacagt
     7501 tcaaggacct caaaaactga ctggacaaaa ggtggtcgta ccaggagata tttccagtgc
     7561 atccttttgg ttagtcgcag gtttgattgt cccaaattct cgtttagtgc tgcagaatgt
     7621 ggggatcaac gaaactcgca ccggtattat tgatgtcatt cgtgccatgg gtggaaaatt
     7681 ggaaataact gaaatcgatc cagtcgctaa atctgctacc ttgattgttg agtcttctga
     7741 cttgaaagga acagagattg gtggcgcttt gattccacga ttgattgatg aattgcccat
     7801 tattgccctg cttgcgactc aagcccaagg tgtaacagtt atcaaggatg ctgaggagct
     7861 caaggtcaag gaaacagacc gtattcaggt tgtggcagac gccttaaata gtatgggagc
     7921 agatattact cctacggcag atgggatgat tatcaaaggg aaatcagctc ttcacggtgc
     7981 tagagtcaat acgtttggtg accaccgtat cggcatgatg acagctatcg cagccctctt
     8041 ggttgcagat ggagaggtgg agcttgaccg tgcagaagcc atcaatacca gctatcctag
     8101 tttctttgat gatttggaga gcttgattca tggctaaggt gttattaggg tttatggggg
     8161 ctggaaaatc gactattgca agaggcttgg accctaatta ccttgatatg gatgctctga
     8221 ttgagaagcg cctaggtatg tccattgcga attttttcgc tgaaaaggga gaagcagcct
     8281 ttcgtcaggt agaatcagaa gtcctagctg atttactaca aacagaccaa gtcgtgtcaa
     8341 ctggaggagg agtggttatt tctcagagaa atcgtgactt actcaagact aatacagata
     8401 acatctacct gaaagcagat tttgaaaccc tctacctacg tatcgcagct gataaggaca
     8461 atcagcgacc gctttttcta aataatagca aggaagaact agtagctatt tttcaagaaa
     8521 gacaggcttg gtatgaggaa gtggctagtc gggttttgga tgtgaccaag ctaagcccag
     8581 aggaaattat agaggaacta agatgaaaat agcttatcta ggtcccaagg gatcattttc
     8641 acaccacgtt gtgcagacag cttttcctca tgaggaattg caggcttttg ccaacattac
     8701 agatgtcatc aaggcttatg agcaaggatt agtgaactat tctgtggtgc cagttgaaaa
     8761 ttctattgag ggtagtgttc atgaaacctt ggactatctt tttcatcagg ctcacatcca
     8821 agcagtagca gaaatcgttc agcctattca tcagcagttg atggtggttc caggccatac
     8881 taagattgaa aagatttttt cacatccaca ggccttggct caaggaaaga aattcatcga
     8941 tgaacaatat ccagaggctc aaatcgaggt aacagctagt acagcttatg cggcccgttt
     9001 tatttccgaa catccagatc agccctttgc agcagttgca cctagaagtt ctgctgaaga
     9061 atatggattg gaactgattg ccgaggatat tcaggaaatg gaagccaatt tcacacgttt
     9121 ttgggttttg ggagctgaag gtgtaactat tcccttgcaa tcacaaactg aaaaaatgag
     9181 tttagccttg accctacctg acaaccttcc aggtgcactt tataaagccc tgtcgacctt
     9241 tgcttggcga ggaattgact tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg
     9301 tgaatacttt ttcattatcg atgtggatta taccgataag gacttggtcc actttgccca
     9361 aaaagaatta gaagcgattg gaatccagta taaaattctg ggtgcctatc ctatttatcc
     9421 aatatcagac catggaaagg agagaagatg agtaaagaaa atcccttaag tcatcatgag
     9481 cagttgcgtt atgattattt gctaaaaaat attcactatc tcaatgagag agaaaaaaat
     9541 gagtttgcct atttgcaaga aaagctaact cttgctaggg gaaatagtag ctctagcttg
     9601 gaacaagaaa gagaagagca ggttgactta ccaagctatg cgaaccggag tcgctcacaa
     9661 tccaaatcac aagcactctc tttccctcca aaaaagaaaa gacggaagct ccgtcttaaa
     9721 cgaattttta tggtgatttt ttcacttttg gtctgtgtgg ctttggccat ggtattcatg
     9781 tttttgcgtg gttaccaaga tgctagcgca aagaaaactg ctgatgcccg ggcagctcaa
     9841 gtagaagtat ttaatggtca ggacactaga gatggagtta atattttaat catgggtact
     9901 gatggtcgaa tcggccagaa cagtgttgag acacgaactg actctattat ggtattaaat
     9961 gtcgggggct cagataagaa aatgaagcta gtcagtttca tgcgtgataa tttggtctat
    10021 atagatggtt atagtcaagt gattaatggt agaaaacaga cagataacaa gttaaacgta
    10081 gcctacgagt taggagaaca agaggggcaa aaaggggcag aaatggttcg ccaagtcttg
    10141 aaagataatt ttgacttgga cattaagtac tacgccttgg ttgatttcca ggcctttgcc
    10201 acagcgattg acacgctttt ccctgatggg gtgacgattg atgctcaatt ttcaacattg
    10261 aatgggcgtc ccctaacaga agctacagtc ggagatgatt tatatgctac cgagactgag
    10321 tctccaaccc aaaccattaa agtcggaaaa cagcagatga atggctcgac cctgctcaat
    10381 tatgctcgtt tccgtgatga tgatgaggcg gattacggcc gtacaaaaag acagcaacaa
    10441 gttttaacag caattttaga gcaaattaaa gatcccacta aacttttcac tggttctgaa
    10501 gctcttggaa aggtttttgc tatgacctca accaacgtac cctatacttt cctcctaaca
    10561 aacggtttat ctgttttgga tggagcaaaa aatggtattg aaaaattgac gattccagaa
    10621 ctgggtgact gggtagatgc ctatgatgtt tatggaggct tgggcttgct ggttgatcaa
    10681 aacaaatatc aaaccaagct cgctcaaatg ggcttgagat aagataatac tcaatgaaaa
    10741 ttaaagtgca aactaggaag ctagccgcag gctgctaaaa acacagtttt gaggttgtag
    10801 ataagactga cgaagtcagt tacctatacc tacggcaagg tgaagctgac gtggtttgaa
    10861 gagattttcg aagagtataa gacagaaaaa tcaaagtttg atagctattt ggatagcagt
    10921 caggctttga ttttttacag tctgcaatag attttcaccc cttgtttgtg gtataatgaa
    10981 acgatacgat agaaagaata atgaacaata tgactgattt aaaagcaatt caggctcgta
    11041 gtctggagat ggctgactat tttgtggcct tttgtaaaga acatgatttg ctctgttatc
    11101 tctgtggcgg aggcgctatt ggtacccttc gaaacaaggg atttattcct tgggacgacg
    11161 acctagactt ttttatgcct cgtaaagatt atgagaaatt agcagaatta tggcctcgtt
    11221 atgcagatga acgttatttc ttgtcaaaga gttacaagga ttttgttgat cgtaatcttt
    11281 ttattaccat tcgtgacaag gaaaccacct gtatcaagcc ttatcagcag gatttggatt
    11341 tgccacatgg tctggccttg gatgttttgc ctttggatta ttatccgaaa gatccagctg
    11401 agcggaaaaa acaggttcgt tgggccttga tttattcact cttttgtgcg caaactattc
    11461 cagaaaagca tggtgctctc atgaaatggg gaagtcgcat tttactgggt ttgactccaa
    11521 aatctctccg ttatcgcatc tggaaaaaag ctgagaaaga aatgactaag tatgatttgg
    11581 ctgattgtga tggcattaca gaattatgct caggtcctgg ctacatgaga aacaagtacc
    11641 caatcacatc ttttgaagac aatcttttct tgccatttga aggaacagag atgcctattc
    11701 caatcggcta tgatgtctat ctcagaactg cttttgggga ttatatgacg cctccaccag
    11761 cagacaagca ggtaccgcat catgatgctg tcatcgctga tatggataag tcttatacag
    11821 aatacaaggg agaatatggt ggctaagaaa aaaatcttat tttttatgtg gtctttttct
    11881 cttggaggtg gtgcagagaa gattctatca accattgttt caaatctgga tctagaaaag
    11941 tatgatattg atattcttga aatggagcac tttgacaagg gatatgaatc tgttccaaag
    12001 catgtacgca ttttaaaatc ccttcaagat tatcgccaaa ccagatggtt acgagctttt
    12061 ttgtggagaa tgagaattta ttttccaaga ctggctcgtc gtttgcttgt aaaagatgat
    12121 tatgatgttg aagtttcttt taccattatg aatccaccac tgttgttctc taaaagaaga
    12181 gaagtcaaga agatatcttg gattcatgga agtattgaag aacttcttaa ggatagctct
    12241 aaaagagaat cacatagaag ccagttggat gctgcgaata caattgtagg gatttcaaaa
    12301 aagaccagca attctatcaa ggaagtttat ccagattatg cttctaaatt acagacaatc
    12361 tacaatggat atgattttca gactattcta gaaaaatctc aagagaagat cgatatcgag
    12421 attgctcctc aaagtatctg tactatcgga cggattgagg aaaataaggg ttctgaccgt
    12481 gtagtggaag tgatacgatt attacaccaa gagggaaaaa actatcatct ctattttatc
    12541 ggggctggtg atatggaaga ggaactgaaa aaacgagtca aagagtatgg gattgaggac
    12601 tatgtacatt tccttggtta tcaaaaaaat ccttatcagt atctatctca gacgaaagtt
    12661 ctcttgtcta tgtctaaaca agaaggtttt cctggagtgt atgtggaggc cttgagtctg
    12721 ggactccctt ttatctctac ggacgttgga ggggctgagg aattatccca agaaggacga
    12781 tttggacaaa tcattgagag caatcaagag gcagctcagg cgattactaa ttacatgact
    12841 tctgcctcaa actttgatgt cgatgaggct agccaattca ttcaacaatt tacaattaca
    12901 aaacaaatcg aacaagtaga aaaactatta gaggagtagc atggaaactg cattaattag
    12961 tgtgattgtg ccagtctata atgtggcgca gtacctagaa aaatcgatag cttccattca
    13021 gaagcagacc tatcaaaatc tggaaattat tcttgttgat gatggtgcaa cagatgaaag
    13081 tggtcgcttg tgtgattcaa tcgctgaaca agatgacagg gtgtcagtgc ttcataaaaa
    13141 gaacgaagga ttgtcgcaag cacgaaatga tgggatgaag caggctcacg gggattatct
    13201 gatttttatt gactcagatg attatatcca tccagaaatg attcagagct tatatgagca
    13261 attagttcaa gaagatgcgg atgtttcaag ctgtggtgtc atgaatgtct atgctaatga
    13321 tgaaagccca cagtcagcca atcaggatga ctattttgtc tgtgattctc aaacatttct
    13381 aaaggaatac ctcataggtg aaaaaatacc tgggacgatt tgcaataagc taatcaagag
    13441 acagattgca actgccctat cctttcctaa ggggttgatt tacgaagatg cctattacca
    13501 ttttgattta atcaagttgg ccaagaagta tgtggttaat actaaaccct attattacta
    13561 tttccataga ggggatagta ttacgaccaa accctatgca gagaaggatt tagcctatat
    13621 tgatatctac caaaagtttt ataatgaagt tgtgaaaaac tatcctgact tgaaagaggt
    13681 cgcttttttc agattggcct atgcccactt ctttattctg gataagatgt tgctagatga
    13741 tcagtataaa cagtttgaag cctattctca gattcatcgt tttttaaaag gccatgcctt
    13801 tgctatttct aggaatccaa ttttccgtaa ggggagaaga attagtgctt tggccctatt
    13861 cataaatatt tccttatatc gattcttatt actgaaaaat attgaaaaat ctaaaaaatt
    13921 acattagaac gggggtgacc aagtgattga tgggaaacga ttattattta gtttgaccat
    13981 agtcagttat gccttgacgc tagtaagtgg aattgtgtat c
//
LOCUS       DAVLYR010000037        13976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLYR010000037 DAVLYR010000000
VERSION     DAVLYR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.037"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            685..1961
                     /gene="ltrA"
                     /locus_tag="VYO63_000878"
                     /pseudo
     CDS             685..1961
                     /gene="ltrA"
                     /locus_tag="VYO63_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2413..3591
                     /locus_tag="VYO63_000879"
     CDS             2413..3591
                     /locus_tag="VYO63_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1671830.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3905..4036
                     /locus_tag="VYO63_000880"
     CDS             3905..4036
                     /locus_tag="VYO63_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1671831.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4057..5607
                     /gene="dltA"
                     /locus_tag="VYO63_000881"
     CDS             4057..5607
                     /gene="dltA"
                     /locus_tag="VYO63_000881"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1671832.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5604..6848
                     /gene="dltB"
                     /locus_tag="VYO63_000882"
     CDS             5604..6848
                     /gene="dltB"
                     /locus_tag="VYO63_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1671833.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6862..7101
                     /gene="dltC"
                     /locus_tag="VYO63_000883"
     CDS             6862..7101
                     /gene="dltC"
                     /locus_tag="VYO63_000883"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1671834.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7094..8362
                     /gene="dltD"
                     /locus_tag="VYO63_000884"
     CDS             7094..8362
                     /gene="dltD"
                     /locus_tag="VYO63_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1671835.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8517..8957
                     /gene="adcR"
                     /locus_tag="VYO63_000885"
     CDS             8517..8957
                     /gene="adcR"
                     /locus_tag="VYO63_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1671836.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8957..9661
                     /locus_tag="VYO63_000886"
     CDS             8957..9661
                     /locus_tag="VYO63_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1671837.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            9654..10460
                     /locus_tag="VYO63_000887"
     CDS             9654..10460
                     /locus_tag="VYO63_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1671838.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSFFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10470..11975
                     /gene="adcA"
                     /locus_tag="VYO63_000888"
     CDS             10470..11975
                     /gene="adcA"
                     /locus_tag="VYO63_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1671839.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13146..13919)
                     /locus_tag="VYO63_000889"
     CDS             complement(13146..13919)
                     /locus_tag="VYO63_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1671840.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
BASE COUNT         4149 a         2580 c         2975 g         4272 t
ORIGIN      
        1 ccggtgaacc caatagcgat tcccaagcct gactatcgtg aggtagcgga ttaaaacggt
       61 ctggggatag accgttttaa gtctgacgca ggaaataaga atcgtcagag gaagggatag
      121 cgaaatcgtg gctctacgaa caggaacgtg atagtaaggc gtatatagcg gataaggagg
      181 cttcaaactc taaagtccaa aaaggtagtc gtaacctata tgtgtaaatc acgagagtaa
      241 ttgaattcgg actaaggttt gtgtgaaaaa gataaatctt tctagagtct aaagactctg
      301 cgtcagattt cctattttca ctgtaacctt ttaacgtcct catatcttgt ataaacgagg
      361 aaagatgtac gacttatccc gtgaggtttc atgagcgctg aaagcgtagt aacaacgaat
      421 catgagaagt cagccgagcc catagtagtg aggaaacttc tgtagtggaa gtggagcgaa
      481 ggggtgaata ctcaaacagt ctggggagag actgtttgag gtctgtcgct agaaagagaa
      541 aacgacagat cgaagtaatc ctacttcact tgtgtctgta aaatgagtgg tctgatagaa
      601 ctggactttg acacgtaatg actagatgaa ggttcaccaa tataagatgt ccctcaggca
      661 ccaaaacaag aaaggaatac gcacatgtca aaactgctag ataagatatt atcacgcgaa
      721 aatatgctgg aagcctacaa tcaagtaaaa tccaataaag gctcagctgg gattgatgga
      781 atgactatcg aagagatgga taattatctc agacaaaact ggcgcttgac taaggaactg
      841 ataaaacaga gaaaatataa gcctcaacca gttcttaaag ttgagatacc taaaccagac
      901 ggaggcatcc gtcaactagg aatttcaaca gttatggata gaatgattca acaggccatt
      961 gtccaagtca tgagccccat ttgtgaaccc catttctcag atacgagtta tggtttcaga
     1021 ccaaataggt catgtgaaaa agccatcatg aagctcttag aatacttaaa tgacggctat
     1081 gagtggatag tggatataga cctagagaaa tttttcgata cagttcctca agatagattg
     1141 atgtccttag tacataacat tatcgaagac ggagatacgg aatccttgat tcgtaagtat
     1201 cttcattcag gtgttatcat taatggtcaa cgttataaaa cactagttgg tacaccacag
     1261 ggaggaaatt tatctcctct cttatccaat atcatgctta atgaattgga caaggaatta
     1321 gaaaagaggg gacttcgatt tgtgcgctac gcagatgatt gtgtgatacg gtcggaagcg
     1381 aggcagccgc taagcgtgtg atgtattcag tcagtcgttt tattgagaaa cggctaggtt
     1441 tgaaagtaaa catgaccaag actaagatta ccagaccaag agagttgaaa tatctaggtt
     1501 ttgggttctg gaaatcatca gatggttgga aaagtcgtcc acatcaagat agtgttcgga
     1561 gatttaagct taaattgaag aaactaacac agaggaaatg gagtatagac ctaacaagac
     1621 gtattgagca actgaatttg tctattcgag gatggataaa ctatttctca ttgggaaata
     1681 tgaaaagtat agtcgccagc atagatgagc gcttgcgtac tcgcctacga gtgattatct
     1741 ggaagcaatg gaagaagaaa tcgagacgat tatggggatt gcttaagtta ggagttccta
     1801 aatggatagc agataaggta tctggctggg gcgaccatta tcaattagta gctcagaagt
     1861 cggtacttaa acgtgctata tcaaaaccag tcctggaaaa acgtggactg gtttcgtgtt
     1921 tggattatta ccttgaacga catgcgttaa aagttagttg aaccgctgta tgccgaacgg
     1981 cacgtacggt ggtgtgagag gggctagaga ttatccccta ctcgattaac tcccctgaaa
     2041 tttattttaa ttatgcaaat ttcacgtatt tttgatgctg agacgacgat cctgggaact
     2101 tttcagatat ttttttgact atctaaatct atcattagaa aagcttagag cgccaaagga
     2161 tttgagcgtt tttctgattt ttaagacttt ttccagtctc tttttcgatt gaagatgtaa
     2221 ttattctact aactaacttc ttagtactag ccaacaacga taatcataat tcctcctaaa
     2281 attaggaata ataaaggcaa tagtttttgt tttttcatgt aaaaaacctc acttttgttt
     2341 tctgctattt tatgctaaaa tattaaaaat caaatttaat tccaaagttt gtaactaaag
     2401 ggggagcgct acatgtctaa ttcatttgtc aagttgttag tctctcaatt atttgcaaat
     2461 ttagcagata ttttctttag agtaacaatc attgctaaca tatacattat ttcaaaatca
     2521 gtaattgcca catcactagt tcctatctta ataggaatat cctcttttgt tgcgagtctt
     2581 ttagttccgt tggttactaa aaggttagcg ctaaataggg ttttatcttt atctcaattt
     2641 ggaaagacta tattattggc gatactggta ggaatgttta ccgtaatgca atccgtagcg
     2701 cctttggtga cctatctatt tgttgttgca atttccatac tagatggttt tgcagcaccc
     2761 gtttcctatg ctattgtgcc acgctatgcg accgatttgg gtaaggctaa ttcagcctta
     2821 tcaatgactg gtgaagctgt tcaattgata ggttggggat taggtggact cttgtttgca
     2881 acaattggtc tgttacctac cacgtgtatc aatttagtct tgtatatcat ttctagcttt
     2941 ctgatgttat ttcttcctaa cgctgaagtg gaggtgttag agtcagaaac taatcttgaa
     3001 attttgctca aaggttggaa gttagttgct agaaatccta gattaagact ttttgtatca
     3061 gcaaatttat tggaaatttt ttcaaatacg atttgggttt cttccattat acttgttttt
     3121 gtaacggagt tattaaataa aacggaaagt tactggggat attctaatac agcatactct
     3181 attggtatta taattagtgg cttaattgct tttaggctat ctgaaaagtt ccttgctgct
     3241 aaatgggaaa gtattctttt tcctcttgta gctatggcaa tagtgacact gactattcta
     3301 tattttccaa acgcacagat gtttttatta ttttcagctt tagttggtat gttatcgcaa
     3361 ctaaaagaag ttcctgaaag tgtatttctt caggaaacag tagaggaaaa tcatttagtt
     3421 aatgtctatt ccgttcttga agtgatttct acattagcat tttcagtatt tgttttgcta
     3481 atgagctata ttactgagag ttttggtatt agcatcagtt tttggctatc agccatttgt
     3541 ctgatgatag aagctatttt aatttatatc agacgagatt attttaaatg agagagtggt
     3601 taaaaagtct ttaaaaatca gaaaagcgca tagtatcagg tgttgaaaaa acttgatatt
     3661 gtgtgtttta ttatgggaag atttacttca ttgtctcctg aaatggagtt ttgaagcagg
     3721 atcactctat ttgaaaatat acatagaaaa actgtataat ccttaaaata agctgaaatc
     3781 tattggaaac atacggtaaa caattgaaaa gatactatat aaagtggtac aatggtagaa
     3841 aaataagcta gtaagaatca ctcttatttt agcgaaaaat tacagaaatg aatggttttt
     3901 cttgatgaaa cagagaaaag aattgtacct ctttcttggt cggacagcct tgtattttct
     3961 tatctttcta gggctgcttt acttctttag ctatcttggt cagggtcaag gaagctttat
     4021 ctataatgaa ttttaacttg aaggagaatc cctattgtgt caaataaacc aatagcagat
     4081 atgattgaaa ccattgagca ttttgctcag acacagccta gctatcctgt ctataatgtt
     4141 ttggggcagg aacacactta tggcgattta aaggctgatt cggatagttt ggctgcagtc
     4201 attgaccaac taggcttgcc tgagaagtct cctgtggttg tttttggtgg ccaagaatat
     4261 gaaatgttgg caacctttgt agcgctgact aagtcaggtc atgcctacat tccaattgat
     4321 agccattcgg ccttggagcg agtttcagct attttagaag tagcagagcc aagcttgatt
     4381 attgccattt cagcctttcc cttggagcag gtttctacac caatgataaa tctagctcag
     4441 gttcaagaag cctttgccca agggaacaac tatgaaatca cgcatccagt caagggagat
     4501 gataattact acattatctt tacttctggt acgactggta agcctaaggg agtgcagatt
     4561 tcacatgata atctcctcag ctttacaaac tggatgatta cggataagga atttgcgaca
     4621 ccgagtcgtc cgcaaatgct ggcacagcca ccttattctt ttgacttgtc tgtcatgtat
     4681 tgggcaccga ccttggcact tggtggtacg cttttcactc ttccttcagt catcactcag
     4741 gactttaagc aactctttgc ggctatcttt tcattgccaa tcgctatctg gacatcaaca
     4801 ccatcctttg cagatatggc catgttgtct gaatacttca acagtgagaa aatgcctgga
     4861 atcacgcatt tctactttga tggtgaagaa ttgacggtca aaacagctca aaaactgcgc
     4921 gagcgtttcc caaatgcccg tatcatcaat gcttacggcc caacagaagc gacagtagct
     4981 ctgtcagcag ttgccgtgac agacgagatg ttagcgactc tcaaacgcct accaatcggc
     5041 tataccaagg ctgattctcc aacctttatc attgacgagg aaggaaataa actgccaaat
     5101 ggcgaacagg gagaaatcat tgtttctggg ccagctgttt caaaaggtta tatgaacaat
     5161 cctgaaaaaa cagcagaagc cttctttgag tttgaaggtc tgccagccta tcacacaggc
     5221 gatgtgggaa ccatgacaga tgagggcttg cttctttacg gcggacgcat ggacttccag
     5281 attaagttta acggttaccg cattgagtta gaagatgtct ctcaaaacct caacaagtct
     5341 cgctttatcg aatctgctgt cgcagtaccg cgctataaca aggaccataa ggtacaaaat
     5401 ctattggctt atgtcatctt aaaagacggt gttcgtgagc agtttgagcg agatatcgat
     5461 attaccaagg ccatcaagga agacctgaca gacatcatga tgtcctacat gatgccatct
     5521 aaattccttt accgagacag tttgccacta actccaaatg gaaagattga catcaaagga
     5581 ttgattaacg aggtgaataa gagatgatgg agttttttca acagcttcct catttagagc
     5641 catatggcaa tcctcagtat tttgtttatg tgattgctgc aaccttgccc atctttatag
     5701 gcctcttttt caagaaacgc tttgcctggt atgaagtgtt ggtaagtctc ttctttattg
     5761 tcaccatgtt ggtgggtgga aagaccaatc aactagctgc cttgggtatt tacctttgct
     5821 gggaaatatt gctcctgctt ttctacaagc attatcgaaa aagcaaggat ggcaagtggg
     5881 tcttctactt agttagtttt ctgtccctac ttccgattat ctttgtcaag gtgcaaccag
     5941 ctatcaatgg aacgcagtct ttgcttgggt tcttgggaat ttcttacctg acctttcgtt
     6001 cggttgggat tatcatcgag ctgagagatg gagtgattaa ggattttacc ctctgggaat
     6061 tcctccgttt ccttctcttc atgccaactt tctcgagtgg tccaatcgat cgctttaagc
     6121 gatttaatga aaattatcag accattcctg agcgagatga gttgatggat atgctggatg
     6181 aatctgtccg ctatatcatg tggggctttt tgtataagtt tatcctagct catgttttag
     6241 gagagacctt actacctcct ctgaagaatt tagccttgca gtcaggtggc ttctttaatc
     6301 tctatgcctt ggcagttatg tatacttttg gtctggagct cttctttgac tttgcaggtt
     6361 attctatgtt tgctttggcc atctcaaact tgatgggaat ccgtagccct atcaacttta
     6421 acaagccctt tttatcaagg gatttaaagg agttttggaa tcgctggcat atgagtctgt
     6481 ccttctggtt ccgtgacttt gtctttatgc gaatggtgat ggtgttaacc agaaagaaag
     6541 tctttaaaaa tcgtaatgta acctcaagca tggcttacat tgtaaatatg ctgattatgg
     6601 gattttggca tggtgtgacc tggtactata tcgcctatgg actctttcat ggactaggct
     6661 tggtcatcaa tgatgcctgg gttcgcaaga aaaaaacgct caataaggaa cggaaaaaag
     6721 cagggaaggc tgccctacct gagaatcgct ggattcagtt gcttggcatg gttgtcactt
     6781 tccatgttgt catgttgtca ttcttaatct tttctggatt cttgaataat ctatggttta
     6841 aaaaataaaa ggaaataaaa tatggatatc aaatcagaag ttattgaaat tattgatgag
     6901 ttgtttatgg aagatgtttc tgacatgatg gatgaagatc tttttgatgc aggtgtcttg
     6961 gatagtatgg gaacggttga gttgattgtg gagattgaga accgttttga cattcgtgtc
     7021 cctgtaacag agtttggtcg tgacgactgg aatacagcta ataaaatcat agctggtatt
     7081 gtggagctac aaaatgctta aacgcttatg gatgatcttc ggaccggtct tgatcgctgg
     7141 tttgttggtt tttctgctca ttttctttta tcctactgag atgcatcata atctaggagc
     7201 tgaaaagcgt tcagcagtgg ctactactat cgatagtttt aaggagcgaa gtcaaaaagt
     7261 cagagcacta tctgatccaa atgtgcgttt tgttcccttc tttggctcta gtgaatggct
     7321 tcgttttgac ggtgctcatc ctgcggtatt agctgagaaa tacaatcgtt cctaccgtcc
     7381 ttatctttta ggacaggggg gagctgcatc gcttaaccaa tattttggaa tgcaacagat
     7441 gttaccacag ctggagaata aacaagttgt gtatgttatc tcacctcagt ggttcagtaa
     7501 aaatggctat gatccagcag ccttccagca gtattttaat ggagaccagt tgactagttt
     7561 tctgaaacat caatctgggg atcaggctag tcaatatgca gcgactcgct tactgcaaca
     7621 gttcccaaac gtagctatga aggacctggt tcagaagttg gcaagtaaag gagaattgtc
     7681 gacagcagac aatgaaatga ttgaattatt ggctcgtttt aatgaacgcc aagcctcctt
     7741 ttttggtcag ttttcggtta gaggctatgt taactacgat aagcatgtag ctaagtattt
     7801 aaaaatcttg ccagaccagt tttcttatca agcaatagaa gatgttgtca aagcagatgc
     7861 tgaaaaaaat acttccaata atgagatggg aatggaaaat tatttctata atgagcagat
     7921 caagaaggat ttgaagaaat taaaggattc tcagaaaagc tttacctatc tcaagtcgcc
     7981 agagtataat gacttgcagt tggttttaac acagttttct aaatctaagg taaacccgat
     8041 ttttatcatt ccacctgtta ataaaaaatg gatggactat gctggtctac gagaggatat
     8101 gtaccaacaa acggtgcaga agattcgcta ccagttagaa agtcaaggtt ttaccaatat
     8161 agcagatttt tctaaggacg gcggggagcc tttctttatg aaggacacca ttcaccttgg
     8221 ttggttgggt tggttggctt ttgacaaggc agttgatcct ttcctatcca atcccacacc
     8281 agctccgact taccatctga atgagcgctt tttcagcaaa gattgggcga cttatgatgg
     8341 agatgtcaaa gaatttcaat agataatata atatagaaga gttcaagaat gaaactagtt
     8401 ttcacgtttt ttcttgactc tttttttgct tgtgatataa ttaactggta aacaaaatgt
     8461 caaagaatag tatttttctc ttaaaaaaga gaagtctaaa aggaaaggag tcagatatga
     8521 gacagctagc aaaggatatc aatgcttttt tgaatgaggt gattttgcag gcggaaaatc
     8581 agcatgaaat cctaataggt cattgcacta gcgaggtggc cctgaccaat actcaggagc
     8641 atatccttat gctcttgtca gaggaatctt taacaaattc agaattggcc cgtcgtctca
     8701 atgtcagtca ggcggcagtt accaaggcca ttaagtcttt ggtcaaggaa gggatgttgg
     8761 aaacatctaa agattctaaa gatgcgcgtg tgatttttta tcagttgact gacttggctc
     8821 gtccaatcgc tgaggagcac caccatcacc atgagcatac acttttaacc tatgaacaag
     8881 tggcgactca gtttactcca aatgaacaaa aagtgattca gcggtttttg actgctttag
     8941 taggagaaat caaataatga gatatattac ggtagaggat ttgtccttct attatgataa
     9001 ggagcctgtt cttgaacata tcaattattg tgttgatagt ggggaatttg ttaccttgac
     9061 tggggaaaat ggagcggcta agacgacgct catcaaggct agtcttggaa ttctgcaacc
     9121 acgcattgga aaggtggcta tttcaaagac aaatacgcaa ggtaagaaat tgagaatagc
     9181 ctatcttcct caacaaattg ccagttttaa tgctggtttt ccaagtacgg tctatgaatt
     9241 tgtcaagtcg ggtcgctatc cgagaaaagg ctggttccgt cgtttgaatg ctcatgatga
     9301 ggagcatatc aaggccagtc tggactcagt tggcatgtgg gaacatcgag acaaacgctt
     9361 ggggtctcta tctgggggac aaaagcagcg agcggtaatt gcgcgtatgt ttgcttctga
     9421 ccctgatgtg tttatcctag acgagccgac aacggggatg gatgcaggaa gtaaaaatga
     9481 attttacgaa ctcatgcacc acagcgccca tcatcatggc aaggctgttt tgatgattac
     9541 ccatgaccct gaagaagtta aggattatgc ggatcgcaat attcatctag tccgtaacca
     9601 agactcgcca tggcgttgtt tcaacgttca tgagaatggc caggaggtgg gccatgctta
     9661 gtttattatc ttacgacttt atacaacgcg cctttttggc ggttattgct atgagttttt
     9721 tctcaccggt attgggaacc ttcctcatct tgcgtcgtca gagtttgatg agtgataccc
     9781 ttagccacgt ctcactttca ggtgtagcct ttggtctggt tttggggatt tctccaactg
     9841 tttctactat tgccattgtc ttgattgcgg cggtctttct ggagtatctc cgtacggttt
     9901 acaagagctt tatggaaatc gggacagcta tcctcatgtc aacaggtctg gctgtttctc
     9961 tgattgtcat gagcaagggt aaaagctcga gttcaatgag tttggaccaa tatctctttg
    10021 gttcgatcgt gactatcagt gaagaacagg tcatttccct ctttgtcatt gcggcggttg
    10081 ttttgatttt gacctttctc tttcttcgtc ctatgtatat cttaactttt gacgaagata
    10141 cggcctttgt ggatggcttg ccagttcgta ccatgtccat tctttttaac atggtgacag
    10201 gggtggctat tgcccttatg attcctgcag caggagctct tctggtatcg accattatgg
    10261 tcttgccagc tagtattgcc ctgcgtctgg ggaaaaactt taaatcggtt atgctgcttg
    10321 ccagtgcgat tggctttttg ggaatggtag caggacttta catttcctac tatgcagaaa
    10381 cacctgcaag tgcaagtatt accattattt ttgtaactgt ctttatacta atcagtttag
    10441 taagacgttt tatcaaatag gagacgaacg tgaaaaaaat tagcttatta ctagccagtc
    10501 tatgtgcctt gtttttagtg gcttgttcca atcaaaaaca ggcagatggt aaactcaata
    10561 tcgtgacaac cttttaccct gtctatgaat ttaccaagca agtcgcagga gatacggcta
    10621 atgtagaact cctaatcggt gctgggacag aacctcatga atacgaacca tctgccaagg
    10681 cagttgccaa aatccaagat gcagatacct ttgtttatga aaatgaaaac atggaaacat
    10741 gggtacctaa attgctagat accttggata agaaaaaagt gaaaaccatc aaggcgacag
    10801 gcgatatgtt gctcttgcca ggtggcgagg aagaagaggg agatcatgac catggagaag
    10861 aaggtcatca ccatgagttt gacccccatg tttggttatc accagttcgt gccattaaac
    10921 tagtagagca catccgcgac agcttgtcag cagattatcc tgataaaaaa gagacctttg
    10981 agaagaatgc agctgcctat atcgaaaaat tgcaagcctt ggataaggct tacgcagaag
    11041 gcttgtctca agcaaaacaa aagagctttg tgactcaaca cgcagccttt aactatcttg
    11101 ccttggacta tggactcaaa caagtcgcaa tctcaggact ttctccagat gcagagccat
    11161 cagctgctcg cttggcagaa ttgacagagt atgtcaagaa aaataaaatc gcctatatct
    11221 attttgaaga aaatgcctca caagcccttg ctaacacact ttcaaaagaa gcaggtgtca
    11281 aaactgatgt cctcaatcct ttagaaagtc tgacagaaga ggacaccaag gctggagaaa
    11341 actacatttc cgtgatggag aaaaacctca aggctttgaa acaaacaaca gaccaagaag
    11401 gcccagcaat cgaacctgaa aaggcagagg ataccaagac agtccaaaat ggttacttcg
    11461 aggatgcagc tgtcaaggac cgtaccttga gtgactatgc aggtaactgg caatcagttt
    11521 atcctttcct tgaagacggc acgtttgacc aagtctttga ctacaaggct aagttaactg
    11581 gtaagatgac ccaggctgag tacaaggctt actatacaaa aggctatcag acagatgtga
    11641 ctaagattaa cattactgat aatactatgg aatttgttca aggtggacaa agcaagaaat
    11701 acacttacaa gtatgtcggt aagaaaattt tgacttacaa gaaaggcaat cgtggcgtgc
    11761 gtttcctctt tgaagccaca gatgctgacg ctggacaatt caagtatgtt cagtttagtg
    11821 accacaatgt tgccccagtt aaggcagaac atttccatat cttctttgga ggcacaagcc
    11881 aagaagccct ctttgaagaa atggacaact ggccaaccta ctacccagat aacctatctg
    11941 gccaagaaat cgcccaagaa atgttggcgc attgatgaga aatcgactag ttcataagag
    12001 ctagtcggtt ttttgatact agatggctaa cttcattata gacctttcag aactaaggga
    12061 acctgaaaaa ataaataaaa ctcttgaaat atctgggctt atatgctaga atgtagttag
    12121 tgtataaggt tcattagaac accaaagccc ctaactagtg ccatagttaa gggctttttt
    12181 gacgtatctt agcgatttaa tgggtgttta taggctagtc agtcggtcta cttcttacca
    12241 tctagccatt tgcagacgaa atacaagata attccagcta taacatctgc tataatagta
    12301 agagcaagct ctggtataag gctcattagt ttcacctcct ctcacgaacc cataggaacg
    12361 taatcggtaa ccgatgacaa aaatagtata ccacactaaa tttagatcat caacctcact
    12421 taatgcttga actattgtag attatatgct aaaatgtagt taattgataa ggttcattag
    12481 aacaccaaag cccctaacta tggccgtagt taagggcttt tttgacgtat cttagcgatt
    12541 taacgggtgt ttataggcta gtcagtcggt ctactcctta ctatctagcc atctgcagac
    12601 gaaatacaag ataattccag ctataacatc cgctataata gtaagagcga gctctatgat
    12661 aaggctcatt agtttcacct cctctcacga acccatagga acgtaatcgg taaccgatga
    12721 caaaaatagt ataccacaat aaatttagat catcaaggtc acttaattct tgaaatatag
    12781 ggtctaggcg acaaagtctt taaaatcaga aaaacgcata atatcaggtg tgcaaaaact
    12841 tgatactatg cgttttattg tgggaaggtt tactccattt tctcctgaaa ttgagttttt
    12901 gtccagcctc tgtttttggg gttgctaaga aaataatgtc atgtggtgaa tatttgtaaa
    12961 tcagtcagca gacagaacga atactcttcg aaaatctctt cacatcatgt cagcttcgtc
    13021 tttccgtata tatgtgactg acttcatcag ttctatctac aacctcaaaa cagtgtttcg
    13081 agctgacttc atcagttttc aaatctgtac tttgagcaag ctgagactag cttcctattt
    13141 gattttcatt gaatatcaga aacccattct ccatcaaata attcgactgc gtctaataat
    13201 ttttgatctg gcacggtgtc tgaaataaag gttgtgtatt tggagagggg attaatttta
    13261 aaaaatccag tcttgtaaaa tttagaacta tcaatcagta agatggtttc atgggctttg
    13321 tcaataatat tcttttttga aatagcttgg ctgagagaag cttcataaac atattggtca
    13381 tcaatacctc ttgctgaaca aaatgctaaa tcgatattaa aatgatctaa taaagaattt
    13441 tccttatcat agttgaccac ggaacaggat tgatgtttga cctcgccaga tgtgataaag
    13501 attttggagc tatctttaac agtttcagat agggtttgtg cagtatgtaa accatttgta
    13561 aaaataatca aattatcaag ttcagaaaga tagggacaga gttcgtagac agtagtactg
    13621 gaatccagat agatacacat accagaccga ataaagtctt tagcgagact agcgattagt
    13681 cttttttgcc tagtactttc tccttcacgt atttgatgag aaagttcaat tgtgttcata
    13741 gaggacaggg tcacgtatcc gtgctttctt ttgataagac cttgattttc taagaaaatt
    13801 aaatcacgac gtaaggtact tgtgctggag aaagtgattt ctgccagctc ttttacggca
    13861 attctttttt tctttttgat aatttcaatc aattcaagta cacgttcatc ttttatcata
    13921 agctcctcct aatttatcat ttcaactata ttatagcaca aattggagga atttga
//
LOCUS       DAVLYR010000038        13745 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLYR010000038 DAVLYR010000000
VERSION     DAVLYR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.038"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(544..1524)
                     /locus_tag="VYO63_000890"
     CDS             complement(544..1524)
                     /locus_tag="VYO63_000890"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1671841.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(1526..3778)
                     /gene="pbpX"
                     /locus_tag="VYO63_000891"
     CDS             complement(1526..3778)
                     /gene="pbpX"
                     /locus_tag="VYO63_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1671842.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(3782..4099)
                     /gene="ftsL"
                     /locus_tag="VYO63_000892"
     CDS             complement(3782..4099)
                     /gene="ftsL"
                     /locus_tag="VYO63_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1671843.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(4111..5061)
                     /gene="rsmH"
                     /locus_tag="VYO63_000893"
     CDS             complement(4111..5061)
                     /gene="rsmH"
                     /locus_tag="VYO63_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1671844.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            5227..5421
                     /locus_tag="VYO63_000894"
     CDS             5227..5421
                     /locus_tag="VYO63_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1671845.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            5437..5979
                     /locus_tag="VYO63_000895"
     CDS             5437..5979
                     /locus_tag="VYO63_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV1671846.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNIIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            5990..6232
                     /locus_tag="VYO63_000896"
     CDS             5990..6232
                     /locus_tag="VYO63_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671847.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            6243..6578
                     /locus_tag="VYO63_000897"
     CDS             6243..6578
                     /locus_tag="VYO63_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671848.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(6688..7689)
                     /locus_tag="VYO63_000898"
     CDS             complement(6688..7689)
                     /locus_tag="VYO63_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1671849.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7749..9650)
                     /locus_tag="VYO63_000899"
     CDS             complement(7749..9650)
                     /locus_tag="VYO63_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1671850.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHYSKDYEKVKRSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKSYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIRVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(9672..9965)
                     /locus_tag="VYO63_000900"
     CDS             complement(9672..9965)
                     /locus_tag="VYO63_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1671851.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(9965..10783)
                     /locus_tag="VYO63_000901"
     CDS             complement(9965..10783)
                     /locus_tag="VYO63_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1671852.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(10770..11549)
                     /locus_tag="VYO63_000902"
     CDS             complement(10770..11549)
                     /locus_tag="VYO63_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1671853.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(11564..12055)
                     /locus_tag="VYO63_000903"
     CDS             complement(11564..12055)
                     /locus_tag="VYO63_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1671854.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(12066..13256)
                     /locus_tag="VYO63_000904"
     CDS             complement(12066..13256)
                     /locus_tag="VYO63_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV1671855.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(13268..13702)
                     /locus_tag="VYO63_000905"
     CDS             complement(13268..13702)
                     /locus_tag="VYO63_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1671856.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         4068 a         3105 c         2399 g         4173 t
ORIGIN      
        1 taaaaaattt agactagcta gatcgctacc ctacgtacta tttctggttt ttctttttca
       61 agcaggagta gactatcttt acttctgaag atttctagat gaaacataga cccacctctt
      121 tctattttac tttaaataag tgttctagta aggctttcat ttaccttacg ttcataatat
      181 actacattgg tcagaaaagg tcaagagttt tgactttaac tgaccaatat ttttttacac
      241 tcttcgaaaa cctcttcaaa ccacatcagc ttctccttat ctacaacctc aaaaccgtgt
      301 tttgagctga cttcgtcagt tctatcaacc atctcaaaac tgtatattaa aacgaaataa
      361 gaacaggaca aatcaatcag gacagtcaaa tcgatttcta acaatatttt agaagtagat
      421 gtgtactatt ctagtttcaa tctactatat tttgagcagc ctaaagttag ctttctagtt
      481 tgttctttga ttttcattgt gtatttaaac acaaaaacac cctgaaacat cagggtgcca
      541 ttcttacatc aaatataaaa ttgctagggt caggagactt gctagaagtc ccactcccca
      601 aaagaagaag tcaaccttcc actcgctcca aggatttcct ttaccagaca atcccccaag
      661 ctcaaaatgg tgatgtacag gcgtcatacg gaaaatacgt ttaccacctg tcagtttgaa
      721 ataactgact tgcatcataa cagaagttgt ttcaaaaaca tacacaattc cgataatcaa
      781 gagagtccat tcttggtgga gagccataga gatagctgcc agcatcccac ctagggccaa
      841 acttcccaca tcacccataa agaccttggc aggcttatgg ttaaagatga agaaaccgag
      901 caaaccacca atcatggcaa gaatcactag aagaatatcc atctgacctt gcacataggc
      961 aataactcca taggcagaca aactaatcac aacggaaata ctagctaaac cgtcaacacc
     1021 gtctgtcaag tttactgcgt ttgaaaaacc gactagccag aaaagagcga agaaaatata
     1081 gaaaaatccc aaatgaactg gataaccaaa gacagacagg atatcgccac cgcgctcata
     1141 gaaaagatag aagataactc cacctagaag ctgaagagct aatttttgct taggattaag
     1201 cccctcattg attttacgaa agaccttgag aaagtcatct aaaaatccga ccaagccata
     1261 caagaccaag atgaacaaaa tcattcccac attattgctg aattggctac taaatagggc
     1321 gaaaaagaaa gcaaccaaaa cagaagtaat caagaaaacc aaacctccca ttgtaggagt
     1381 cccagctttt gcctgatgct gtttgacatc ttcatgcatc tgctgtcctg taatttgcgc
     1441 ctttctataa aattggataa aggccggaat tcctactaaa gttagtaaaa atgtcacaat
     1501 tccagcactg atggaaataa acatattagt ctcctaaagt taatgtaatt tttttaatgt
     1561 ccttgatagc tgtgttagca cgaacatctt gcttctgcac agtagagccc gaaccttgaa
     1621 attcaagttc tatattgagc cacttagcaa gggtctcagc agtctccttt gtccaaccat
     1681 acatatctgg aacttcctct gctttatcag ataagataag gacttgctga ttcggggcaa
     1741 gattcttccc ttcttcagca gaactgtttt taatcttcgt tcctgttccc acaacgatgg
     1801 gttgtacaag attgcgacgc aattcttctg ctaaatcacc aggtgaaata tccttgacac
     1861 taggcatagg ataaggactt tgttgactta cttgctccaa agctttagct gttgtttgaa
     1921 gattgagaga gtctttcata gctgaagccc gctccaagat aggattggca aattctccca
     1981 actgaatacc tgaataatgt tcaggttgtt ggaccgtcac atacaagata aaatcaggat
     2041 tttcagccgg actcatcgat acagccgaga aaatatagtc ggttaccccg actagataac
     2101 caccattttt ctcgtcagca atctgagccg taccagactt gagggctaca ttttgcccag
     2161 gaacagttac agttggcttg cctgtgctgt ggttatacat ggttccataa accggatccg
     2221 tccctaccaa aaccatgtta gtccgagtta gactagctgc atctttagaa acaggatttc
     2281 ccacaatttc tttttgagat ttccgagcag tttgatcatt tggatcataa atggcactaa
     2341 taaatttagg ctccagcatg acaccgtcat tagcaatagc tgtaaaggca cgaatcattt
     2401 gcgtctgggt cactgaaatc ccttgtccaa atgagctttg cgcaatgttg acaatattat
     2461 ccgcaggaag ctgaccagca tactcatccg tcaaaccgaa acgggtcgga actccaaatt
     2521 taaaacgatt aagataatca agccaggtag catctcccat cttttgctca aggagggtca
     2581 tcccaacgtt acttgagtgt gcaaaacctt gagaaaaagt catcattctg ccaccagtca
     2641 atccttcatt aacgtcccaa tctcgaatcg tggcatctgc aatttttaac tcactactat
     2701 taaagacttc tcctcctgga aaggtattat tatcaatagc agcagccaac atcatcactt
     2761 tcatagtgga acctggctca tagttacttt ggtaaaggat atcacgccaa acaaagtcct
     2821 ctgtaatgcc ttcttttgta tctgcatcaa aggtcggtcg ttgcgttgtt gccagaattt
     2881 cccctgtttt agcactgacc aaagtcgctg tcatgtactt tccttttacc ttctcttgaa
     2941 aagcatccat ctgggtttcc ataaaggact ggagggggct ggaaatggtt gtataaacat
     3001 ccttaccgtc catcgttcgt tgggaaactt gttctgttcc gggtacaata ttacccagac
     3061 gatccttttc ataggtaata atgccgtctg tccctgcaag aatactgttc aaggaactct
     3121 ccattccaga ggttcccagc aagctcttgc ttccatcttc attttcatgg agctgagcta
     3181 ggccgataaa actagaagca aattgtccgt ttgggtaact acgattggga ctggttgtaa
     3241 aatcaatccc cttgacctct gcagcttcca attctttttt gatagacatc atattggcat
     3301 aggtaatccc atttcccttt gctccaaagg aaacttgctt gagattaggt tgcgagagtt
     3361 gctctcttac ataggattct tccatgtcca aatacttatg aaagacctct gcaaccttgt
     3421 taaattgtgt tttttctacg taaagaatct tacccgttgc tgacttatag ttctcatcaa
     3481 tgaccgcata gacattatag gaggttgcat cctcagcaat cgggactcca tttcggtcat
     3541 aaatagtccc acgtttggca ggaactgtac gggtggtttg atgaactttc ttagcttcct
     3601 tcgctaaatc tgttccaaag cgagtgcctg tcccaataat gaccgcaaaa ttgactaaaa
     3661 aaatggcaaa aacaaagaca gataataaac tcagactttt tccaactctg cgtctgtttt
     3721 cagccggcga tttccgattt ttggtcgcat aacggattac tctttttgtc cacttcatat
     3781 cttactccgc tattctaata ttttcattgt ttaattgcaa atcgtgtgaa ttggcaattt
     3841 ctttcaaacg ttctgcacgt aatagttcat tgacctcttg cttggcatcg tccaattcgg
     3901 ttttcttttc ctctatctgc gcattgattt ttgtcaaatc attctgcact tgcaagagct
     3961 tggtctgcat aaaaataata ctaatggcta caataagagt ggttacagca atggaaaagt
     4021 aaaaagcttt ttccacacgc gaaaaccgtt taagttgcat ctgtagtatt tgacctgttt
     4081 tttccatttt ttctgccatc tttttccctc ttacttgtga atttttctgg ccacgcgcaa
     4141 cttggctgag tgcgagcggt tattggcttc taactcttcc gcacttggca agattggctt
     4201 acgggacacc aattccatct tgggcttgag atcatctggg atgaaaggca agccttttgg
     4261 aacttcaact gttgaagctt ccttgaacaa ttgcttggtc aagcggtctt ctaaggaatg
     4321 aaaggtaatc actgaaattc taccatccag agccaacata tccatagcct gctggatgga
     4381 ctcatctgcc gcccccagtt catcattgac ttcaattcga atagcctgga aaatctgctt
     4441 agcaggatgc cccttcttct tgagttcctt ggcaggtttg accaacttga taatctctgc
     4501 taactcagtc gttgtctcaa tcggcttcac ttcacgcgct tgctcaatct tacgcgcaat
     4561 ctgtttagag aatttgtcct ctccatactt gaagaaaata cgaaccaagt catgatagtc
     4621 ataattgttc accacttcat acgctgtcag actagcatcc tgattcatcc gcatgtccag
     4681 tggcgcatcc tttttataag aaaaaccacg ctcacgctgg tctaattgag gactagacac
     4741 tcccaagtca taacaaattc catcaatttc ctgaacacca gcttcgcgca aacgtgcctg
     4801 taaatgacgg aagttgtcct tgataaaggt caccattccc ttctcaatgt aaggtgccaa
     4861 gcgtttttgc gcattgtcaa tggcattctg atcctggtca aaggcataga gatggccttt
     4921 ttcacttaat ttacttaata aatactcgct atggcctgct ccacccaaag tcgcatcaac
     4981 gtagataccg tcaggtttta cgtcaagcat atcaatcgtt tcgtggagta agaccgttac
     5041 atgatgaaat tcttttgtca tatcttatct attttaccac aaatccgacc agcttgcact
     5101 tgtcagacaa ataggacaag gagactacta tttcttcaaa aaatatgtca aaaatacttg
     5161 acactttatg tttaaaagac tataatatag tcaaatatat acgacataaa tcagaaagga
     5221 gaccagatga atcgtgtgaa agaatttcgc aaggaattgg gtatttccca gctcgaactc
     5281 gccaaggata tcggtgtctc gagacagact atcaatatga ttgaaaacga caagtacaat
     5341 ccaaccttgg aactctgtct caatctcgcc cgcagcctcc aaactgacct caacagtctc
     5401 ttttgggagg aagattttta aaaaaggagc aaactcatga aaaaagaaac cttcactgaa
     5461 aaactgatca aacgcacata cggtatttct ggtccccttg acgaatacaa acggcgtgag
     5521 gccgatagta ttgggaacca agtctttatc gtcctctttt atctgatgat tttcggaaat
     5581 attattccac tccttctggc ctataaatac cctcaagaag tggctctaat ctatcctcct
     5641 ctgattttag tgattgccct catcgcttct ggctatgtca cctaccaaat gaaaaaaaca
     5701 ggcatcacag tcattgaacc agatatgctg aatgagaaag aaagcaagca actacactac
     5761 cctggtctga aagcaggttt gttctttggt ctatggatgt tttttataac tcctcttctc
     5821 agtatactca tagatgaagg tcaggactat tttcattctc ttctcactat aagaaatggt
     5881 gtatcaagta ttctcggttc tattttcttc ggagcgagca tacagttcct catctcccgt
     5941 cgcattgcaa aaactaagaa aaatcaagat gaggattagg aggaaactca tgaaaaaaga
     6001 aaagaaacag ttacgttatc ctggtctgaa agcaggttcg atttatggaa cagtgatatt
     6061 ttttataatt ccactcattg ataccctaac aagtgaaaat ccaaatttta tcagttctct
     6121 tctaaataca aaacatattt ttaaaactat actgggagct ttctttttcg gagtgatgat
     6181 acatattgtc gactcacttc gtattgcaag agctaaaaaa gaccaggatt aggaggtgcc
     6241 ttatgaaatc actagctaga ctactgatca ttcatgtttt tatcagtatt ttccttttct
     6301 tcgcccttac ttcaggagct atttctcata cagttttact actcctactc ctctttcttc
     6361 ctgcgctcaa taaaggactt gagaaaatac aatcaaaacg gatacctgtc ctcaacgcag
     6421 ccctcttctt tctcctcata tcctttccac aacttttaac caaccctgtc caatggaaat
     6481 tttcaatatt cctagtcgta accatcattt caagtttggc ctacttctat aacttttatc
     6541 aagtagttaa agaagtagat caaaaacagt tgatttagga gcttgctccc ataatttctc
     6601 attttatagg caaataatcc cacctagctt gttctaggtg ggattatttt atttcttaac
     6661 agagattgca agtcattttc cttcatttta gaaagtcgac tctttccaat tcacactaca
     6721 atccaagact tgttgccttt cttcttgatt gcgaccttca atctggtcaa tcaaaatctt
     6781 tgtagcctgt tgtccttcct caaaggaggg ctgaaccagc gtcgaaacac ttggagaaga
     6841 aaagcaagtc cactccgtat tgtcaaaacc aatcaaccca acttgcggca agttataatt
     6901 caactctttg ataacggtaa agactagagg tagggcccaa cagttaggga taaataccag
     6961 agttttttca tcgggatcga tttctttttg taaaaattct ttaatttgtt ccaaattcgt
     7021 atgcttatct tcaatggtta gactggcgtg acgcatatta gcatctgtta aagcatccac
     7081 aaaaccactt gcccgctcaa tccgagtact caaacgactc gtatccgctg taatcaagag
     7141 aaaatgttca taaccttttt cgatacagga ctgggtcatg tcataaacgg catcatagtt
     7201 attggtttta acccagctag tccggtgttc atagagctga ctatcaaaaa agaccatttt
     7261 cttctttttc tcatcgatga tacgagaata ttttcggaaa ttagaggtcg gctgaataat
     7321 aaagccgtct actcccaaga gaagcatgct ttcaatatac cggtcctcac tctcttggct
     7381 gtaattacta tttcctatca ttacctggta gccattctgg ctggcgatat cctcaattcc
     7441 cttaacaatt tggtttgaga aactgttggt aatatcacca atcaaaacac cgattaattt
     7501 tgttcgtttg gagtttaagc tacgcgcaac aatgctcggt ttgtaatttg tttcatgaat
     7561 aactttttca atcttttcac gtgtctcttg ggacattttt tcatatttcc cgtttaggta
     7621 aaatgacacg gttgttttcg aggtctgagc catttccgca atgtctttta tggtcagttt
     7681 cttctccaat ctaaaacacc tcctactaat acttattata acatattcac tattcaaaat
     7741 gcctatttct agcactgcaa gcgaatcatt ctctcattta ttttatcata tactagacat
     7801 tttccacgca tttttgttcc gtcaacgagg cacaattttt cacctacacg aatatcctcg
     7861 cttaataaca gaatcagctt gtctgtttcg tcatttttca cttcaaaagc cagagcattt
     7921 tccacttcac gatcatctgt ctggtagaca gaatgacgga tgatttcaaa actctcatgc
     7981 gcaatgatgg tataatccag catctgattc tcaaagaatt ggcgcttggt gagtttgcta
     8041 cttctttcaa gctcattgta tttaggagaa atgatggtat cttccaaatc aaaatcaact
     8101 tcactccata gtctcaactg attgattttc ccatcttgat aggtcacatc cttgtcaagg
     8161 ataaactgag tcaacgcctc atgctgacct tgacacctga tgtcatctac caagagccag
     8221 acatcctcta ccaacatgag gatttttctc ttgtgaagat aaggcaaatc aggttctgct
     8281 gaccaataag ccccttcaat ataatgcact ccctcccttt ctttatggtg acaaaacagg
     8341 gagtgaggat agtattcata ttcccaggat cccgtgattc tttccggagc tttcccatct
     8401 acaatgcagg tcgaatgact ccaagcactc tttaagagat aacgttcata tatctcccga
     8461 taagaataac gcccagcatc tatgaaaata ggttggcctt gatactgtaa gcaaaaacta
     8521 ttctcgtcac tatggctatg ggcacttcct agcggaccat ttttgaaaaa tagataacga
     8581 tgttcatcct taatgcagac atgtccagag tcttcaaaga tcatggactt aggctgccaa
     8641 gctctccttt caaattcctg cagtcgcttg accttttctc gccccaggaa caagaggcta
     8701 agcaaatcga ctttaacatc cagaccgtta agaaggtctt ccttgttcaa aaccacagca
     8761 gacagactca aaatttctgt cgtttctgta gaatcgctat caccaaaagc caaagttcgt
     8821 ccatctaagc ctgtcatcat ttgaatatag gtcgccatct tttccagcaa ctctcggtaa
     8881 ctatcttgca agtctggaag caagagacac aaatccagca aggctttata aacctctaca
     8941 tgatagagaa tcgactgttc aaactggctt ccatctccta aaatctgtgt ctcaatttgc
     9001 tgtttcaact cctctgaagc aaaatggtaa gcttcttcta gatccatctt atctgaaaag
     9061 aaataataga tagcaagcat cggaattgtt tgtaaaatcc cccagttact aagggtgtac
     9121 ttggcgcgat agtggctttt cataaagtca atctgctttt ctagactgac caaaattttc
     9181 tctagttctt tctcctctag caagtcaaat ttcaagagga gcaagagtag tttcaaccaa
     9241 gtaaaggaac gaatacccgt atccaaggtt ctagtcatca aggattgagg agaaaattct
     9301 ctcacctgct caatccaatc aaatagaaag aacttgcact tttgaatata gctcttatct
     9361 ccttctacca cataccctat cataaactgc aagagatatt cttgtcgatt gagcatataa
     9421 gcccattctg gatcatcttc aaatacttga tcccatacca tcggctggat ttgatggatt
     9481 tttgaacaag gctccatatc ccaaggacta tcaaacataa aacgattgtc catcaagcgt
     9541 tcaagggaac gcttgacttt ctcatagtct tttgaatagt gggacaagat ataatcacga
     9601 cattggtttc catcgattct ttcaaaaaat tgtcttcttt cttctttcat tatctattac
     9661 cagaaaaaga actacttaaa aagcagttct tttgtctttc ccattacact ttccttttct
     9721 acatagatga ccacaccttt tgcaatctgc aaggagacca agtcatcttt gatagaaatg
     9781 atttttccat gaattccaga caataacaac acttcatcac cagatgttaa agaagctaaa
     9841 tactcttgtc gttgctccat ctgtttgcga agcaactttt gctgacgaat agaatgaaag
     9901 cttgacagta aaaggggact cactgccaag acaatcacta ttccataaaa caatgttgta
     9961 tccattaagc tataatctta agccagcttc cgataattcc gatgataact gttaaaataa
    10021 cgagtttata tgttgtccat ttcttttctt tgatcaagta gtaaactaaa agtgtaaata
    10081 gggctggtag aagagctgga gcaaccttat caagcattcc ctgaatactt acgatacttt
    10141 gtttagcgtc tgctttaact tcccctgcag caaaggtaat cggcaccata atcttaacag
    10201 atgtcgctgc caaaccagca attacggtta caccgataat attggcaata cgagaaatcg
    10261 ttgccatctg ctcgcttagt ttatcaatca cagttgttcc tagtttgtat ccatacagac
    10321 cagttgacaa tttaatcgct gttaaaatcg tattcatcgc aaggaagaac aagattggac
    10381 cgacaaccaa gccttcttga gcaaacgaag ctgcgatggt tgagaacaat ggagctaaac
    10441 agaattgaga aagagaatcc ccaatacctg ccaatggtcc catcaaggcc atcttgatgc
    10501 tacgtgtttc ttttgttggt cgaccatttt ccaacattac aagatgcaag ctggtaataa
    10561 aaggcaggaa gtgtgggttg gtattataga attcacagtt ttcttccaag gcttggtaga
    10621 aaccttcctg atcctctcca tagtgttttt tcaaagcagg atacatcaca ttggcatatc
    10681 ccaacccttg atagttacta tagttaaatc cattttgaca aaagaatgcc cgcaaagacg
    10741 ttttaagata atcacgtttt gttaatttgt tagatccagt catcgtgtgc ttcctcctct
    10801 accacatgat ccgctgtttt tggcttgtta taaaattcaa tcaaagcaaa gatagtacct
    10861 acaattgcaa taccaattgt tgggatgttt agataagctg cacaaacata tcccaacaag
    10921 acaaagggaa tcaactcttt cttagccatc actgacaaga tcatcgcaaa accgatagct
    10981 gggagcattt taccagcaac tgtcaaacct gtaagtaata ccggtggaat gtagtctacg
    11041 agtttcaaca aggtatccat tgaaagggca cccaagcaac ccaaggtaaa tccaataaag
    11101 gcaaacaacc aaattgttgc atttagagtg aacttaaatt tcttcaaatt atggtttttc
    11161 aagtgcttca tcgctgtttc aggcgcacca gcacgtacag tgtaggcgaa agtttgtaag
    11221 aattgaatcg ccacagcaat cggagtagag agggcaagag ccgcttctgg actgacttta
    11281 ccagcactag tgatagccat caaggtacca aagataccag gtccgattgg gtttggtgga
    11341 acagtacctc cagcacctac accgaatccc atataagcca attcaccaat agctcccatt
    11401 gcaagagcag taggcagatc acctagaata attccgacac caaatgacag aacaatacat
    11461 ctattggtgt aaattcctaa cagcattcca ctaaaacaga aagctgtcca taatccaatt
    11521 aaaatcgctt gaaatacatt aatcgacata acgatctcct cttttaaata tagtccataa
    11581 tgttgacttg aacagctcca tcatttcctg ttggagttgt tttcgtatta aatgttactt
    11641 gatgagtttg gctcaattcc ttgagggctg ccttgtcctc ttcacccagg aagatggagc
    11701 gtgtcacttg ctctttacca ggggcattgt gaatgttccc aatattgatt tctttgatag
    11761 ggacaccacc ttctaccaag gttaaagcgt cctttacatc ctttacaacg ataaagatcg
    11821 tttgagcagg attagccttg tgaatgatat caatcacctt ttgcaaaggg aagaaacgca
    11881 tggcaactga gtctggcaca actgttttca tcagagtttg ttgcatcttg tccgtgctta
    11941 cttcgtcatt ggcaacaatg accgtattac aacctaggta ttttacccaa agttgtcctt
    12001 gtccatgaat caaccgttca tcgatacggg tcataataat atttggcatt gtcatatttc
    12061 tcctcctacc aatataggtt ccagtctttg tagaaacgga taagggcttc taggtaatag
    12121 tagtcacccc agatattgcc ttcatccact cctttacctg aatgccatga gtacacaccg
    12181 tggaggagac ttgtcccacc aggggtaaat tgatcatttg cataatgttc gatcaaggaa
    12241 cgaagcatgg catgcatagc atgtttataa atatctttgt cagcatccac ctctgggaga
    12301 tgttttagca tttcatgaat cccacagacg gcgatagctg ttgctgaaga atctcgtgat
    12361 tgatcactac catcattaaa aatcaaatcc caataggaca catgatcttt tggcagacga
    12421 ttcaagaaat aattggtcac acccttaaac aagtcaaagc aggactcgtc ttttaagtga
    12481 cgataagtca aaggaatacc atagactccc catgattgac cacgtgccca gcatgaatca
    12541 tcactatacc cttgtctcgt tacaccttta aagggttgac ctgtctcagg atcaaaatag
    12601 aaggtgtgga aggacgaagc gtcatcacgg attacattat tagctgaagc atagaaatga
    12661 ctttctgcaa tatcgtagta tttttgatca cctgtttctt gataagcaaa gaataagagt
    12721 tggatattga gcaagcagtc gataatcaaa cggtaatgct ctttcttgcc caagtctccc
    12781 caagcttgaa taaaaccacc tttttcttga tagcgttcaa tcaacttatc tgcagctttc
    12841 aaggttgctt ctctagcctc tccatctcca tttatcttat attcagccat acaagacggt
    12901 gtgtacaaga agccgagatc atggtgatcc aattctactc tcttattgac acgatccagg
    12961 aaagaaagaa catttttatg agcgatgttt ttaaatgcat cctgttgact gtattcataa
    13021 gccaaccaca gttctcctgt ccagaaacca ttggtccatt ccgtgttatc catgattgga
    13081 tagacattat caaaggtagc tggcgtcggg aaatcttcct tgaaatagtc aaggttgagt
    13141 tctaactgcc gaataacctt atcgattgcc tggccgactt cttctttcgt cagaagtggt
    13201 acttctaaga agcgctcagg cgattttatt ttttcaatcg taaccttttt tatcattctt
    13261 ttacccttta aatacccgat tcgaaatctt cttcctcttc ctctgcatcc gttgaaaaca
    13321 attctttacc atttacgact ccgccctggg ccgccactac tgatttatta acaacctcat
    13381 caaagctatg agccattcta gcaaagactg cttccattaa catggctaag ttcaaacccg
    13441 agagaacatt cattgttttg gctggatttt ctcccattat ggtagaagaa accttgaatg
    13501 gcgatcctcc caagagatca cttaggatta aaacttcttc ttcatttgaa attgcaagta
    13561 agattttttg cttgagttca tctgctgaca ttccttccac aaagtcaatc gcctccacat
    13621 tttcttgatt gcctgcaatc aattgtaaag aactataaat ccctgtagca aaatgtccat
    13681 gccctacaag tacaattttc atcttttctc ctttcttgtt taggtttacc ggtttacttt
    13741 atatt
//
LOCUS       DAVLYR010000039        13558 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLYR010000039 DAVLYR010000000
VERSION     DAVLYR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.039"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(156..233)
                     /locus_tag="VYO63_000906"
                     /pseudo
     CDS             complement(156..233)
                     /locus_tag="VYO63_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            304..960
                     /locus_tag="VYO63_000907"
     CDS             304..960
                     /locus_tag="VYO63_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1671857.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            950..1273
                     /locus_tag="VYO63_000908"
     CDS             950..1273
                     /locus_tag="VYO63_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1671858.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKHKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1376..2206
                     /locus_tag="VYO63_000909"
     CDS             1376..2206
                     /locus_tag="VYO63_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1671859.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2360..3214
                     /locus_tag="VYO63_000910"
     CDS             2360..3214
                     /locus_tag="VYO63_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1671860.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3314..4687
                     /locus_tag="VYO63_000911"
     CDS             3314..4687
                     /locus_tag="VYO63_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1671861.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4680..5741
                     /locus_tag="VYO63_000912"
     CDS             4680..5741
                     /locus_tag="VYO63_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1671862.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5743..6435
                     /locus_tag="VYO63_000913"
     CDS             5743..6435
                     /locus_tag="VYO63_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671863.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6464..7033)
                     /locus_tag="VYO63_000914"
     CDS             complement(6464..7033)
                     /locus_tag="VYO63_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1671864.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7168..7782
                     /locus_tag="VYO63_000915"
     CDS             7168..7782
                     /locus_tag="VYO63_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1671865.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7784..9430
                     /locus_tag="VYO63_000916"
     CDS             7784..9430
                     /locus_tag="VYO63_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1671866.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9442..10728
                     /locus_tag="VYO63_000917"
     CDS             9442..10728
                     /locus_tag="VYO63_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1671867.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10780..11313
                     /locus_tag="VYO63_000918"
     CDS             10780..11313
                     /locus_tag="VYO63_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1671868.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11391..11861
                     /gene="nrdI"
                     /locus_tag="VYO63_000919"
     CDS             11391..11861
                     /gene="nrdI"
                     /locus_tag="VYO63_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1671869.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12154..13416)
                     /locus_tag="VYO63_000920"
     CDS             complement(12154..13416)
                     /locus_tag="VYO63_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1671870.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIIIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3951 a         2545 c         3114 g         3948 t
ORIGIN      
        1 ctatataata gtccatgctg tggctggatt cttttcagcc tacttgtact cttcgaaact
       61 ctcttcaaac cacgtcagct tcactttgcc gtagtacggt tactgacttc gtcagttcta
      121 tccacaacct caaaacagtg ttttgagctg acttcgtcag ttctatccac aacctcaaaa
      181 cggtgttttg agcaacctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat
      241 tattaagaca atatgaggag aaagatgaaa gaaaaaggat tttaggaggg ggcgcaggca
      301 gccatgccaa cggctcttgg ctatgtcagt atcggcctgg cctgtggaat tatcggtgcg
      361 ccctatgtga cacttgttga gatgggcttg atgagtctct ttgtttatgc tgggagtgcc
      421 cagtttgcca tgttggcact gattgtggtt caagctcctg tggcagctat tgctatgacg
      481 gtttttctaa tcaacttgcg tctctttttg ttgagtttac acgcatcgac ttatttccgt
      541 cataccagtc tttggtacaa tatcggtatg tctagtatct tgacagatga gacctatggc
      601 gttttgatgg gtgaattggc ccatacagac aaggtaaatc ctatgtggat gcacgggaac
      661 aatctcaaca gctatgtggc ttggtttgtg gggacagtag tcggaacggc tctgggtggc
      721 ctgctaccaa atccagaaat ctttggcttg gattttgccc tggttgggat gtttattggt
      781 atttttgctt cgcaatttca gattatgcaa agacggattc ctgtccgcaa tctgctcatt
      841 atcctagcag ttgttgcggt gtccttcttt ttgctcttga cagtggtgtc tcagtcacta
      901 gctgttctgt ttgcgacgct acttggttgt agcatggggg tggttttaga tggtcagtaa
      961 gtatctttta ttagcagtta ttttctctgg tcttgtgacc tggattcccc gtatgattcc
     1021 cttcatcttg gtcaagcata agggcctgcc tgcgattgtt gagcgttttt tgaagttttt
     1081 gcctgtttcc attatctttg ccttgattct ttcaagcgta gtgacaggta aggttgggag
     1141 ccttcctcaa attaaatggc tagacttctt agccgtcttt ccaacagctt gggtagcctt
     1201 tcgctaccgc aatctagtcg gaacagttct ctttggagtg gtcttgattg ccatcttgcg
     1261 tttggtcttt taaattaccc ataaaaaaac ttatcacaga gatagatatc atataatggc
     1321 gtaaatgctc tttttctgtt aagattataa ggtattctat tttggaggaa atgacatgaa
     1381 aaaaatcgtt aaatactcat ctcttgctgc cctaggactt gttgctgcag gtgtgcttgc
     1441 ggcttgctca gggggtgcta agaaagaagg agaagcagct agcaagaaag aaatcatcgt
     1501 tgcaaccaat ggatcaccaa ggccatttat ctatgaagaa aatggcgaat tgactggtta
     1561 cgagattgaa gtcgttcgcg ctatctttaa agattctgac aaatatgatg tcaagtttga
     1621 aaagacagaa tggtcaggtg tctttgctgg tcttgacgct gaccgttaca atatggctgt
     1681 caacaatctt agctacacta aagaacgtgc ggagaaatac ctctatgccg caccaattgc
     1741 ccaaaatcct aatgtccttg tcgtgaagaa agatgactct agtatcaagt ctctcgatga
     1801 tatcggtgga aaatcgacgg aagtcgttca agccactaca tcagctaagc agttagaagc
     1861 atacaacgct gaacacacgg acaacccaac tatccttaac tatactaagg cagatttcca
     1921 acaaatcatg gtacgtttga gcgatggaca atttgactac aagatttttg ataaaatcgg
     1981 tgttgaaaca gtgatcaaga accaaggttt ggacaacttg aaagttatcg aacttccaag
     2041 cgaccaacaa ccgtacgttt acccacttct tgctcagggt caagatgagt tgaaatcatt
     2101 tgtagacaaa cgcattaaag aactctacaa agacggaacg cttgaaaaat tgtctaaaca
     2161 attcttcgga gacacttatc taccggcaga agctgatatt aaataatttc ttgaaatcat
     2221 ccattctgag taagatgggt ggtttctcag tttttaggga tatagtttta aactatatat
     2281 ctgactatct aataacaatt tgtgaaatca aacaaattgt gagatactag tacggtatta
     2341 tttttaagga gaaagaatca tgaaaatcaa aaaatggctt ggcctagcag cccttgctac
     2401 agtcgcaggt ttggctcttg cagcttgcgg aaactcagaa aagaaagcag acaatgcaac
     2461 aactatcaaa atcgcaactg ttaaccgtag cggttctgaa gaaaaacgtt gggacaaaat
     2521 ccaagaattg gttaaaaaag acggaattac cttggaattt acagagttca cagactactc
     2581 acaaccaaac aaagcaactg ctgatggcga agtagatttg aacgctttcc aacactataa
     2641 cttcttgaac aactggaaca aagaaaacgg aaaagacctt gtagcgattg cagatactta
     2701 catctctcca atccgccttt actcaggttt gaatggaagt gccaacaagt acactaaagt
     2761 agaagacatc ccagcaaacg gagaaatcgc tgtaccgaat gacgctacaa acgaaagccg
     2821 tgcgctttac ttgcttcaat cagctggctt gattaaattg gatgtttctg gaactgctct
     2881 tgcaacagtt gccaacatca aagaaaatcc aaagaacttg aaaatcactg aattggacgc
     2941 tagccaaaca gctcgttcat tgtcatcagt tgacgctgcc gttgtaaaca ataccttcgt
     3001 tacagaagca aaattggact acaagaaagc acttttcaaa gaacaagctg atgaaaactc
     3061 aaaacaatgg tacaacatca ttgttgcgaa aaaagattgg gaaacatcac ctaaggctga
     3121 tgctatcaag aaagtaatcg cagcttacca cacagatgac gtgaaaaaag ttatcgaaga
     3181 atcatcagat ggtttggatc aaccagtttg gtaataagaa acagggaggt gggagagaaa
     3241 attccacctc ttgcttttgt atagagtata gattgtaaag aagactattc gttcatagaa
     3301 aggtagagag aatatggttt ttcctagcga acaagaacag attgaaaaat ttgaaaagga
     3361 tcatgtagtc caacattatt ttgaggtttt gcgtaccttg atttctaaga aatcagtctt
     3421 tgcccagcag gttggactca aggaagtcgc aaattatctg ggtgagattt tcaagcgtgt
     3481 tggagctgaa gtggagattg atgagagcta tacagcgccc tttgtcatgg cacatttcaa
     3541 gagttcgcgt ccagatgcca agaccttgat tttctataac cactatgaca ctgtgccagc
     3601 ggatggggat caggtctgga cagaggatcc ttttacgctt tcggtccgca atggcttcat
     3661 gtatgggcgt ggggttgatg acgacaaggg tcatatcaca gctcgcttga gtgctttgag
     3721 aaaatatatg cagcaccatg atgatttacc tgtcaatatc agctttatca tggagggagc
     3781 ggaggaatcg gcttcaacag acctagataa gtatttggaa aagcatgcag acaaactccg
     3841 tggggcggat ttgttggtct gggaacaagg gaccaaaaat gccttggaac agctggaaat
     3901 ttctggtggc aataagggga ttgtgacctt tgatgccaag gtaaaaagcg ctgatgtgga
     3961 tatccactcg agttatggtg gtgttgtgga atcagctcct tggtatctcc tccaagcctt
     4021 acagtctctt cgtgctgcgg atggccgtat cttggttgaa ggcttgtacg aagaagtaca
     4081 agagcccaat gaacgagaaa tggccttgct agaaacttat ggtcaacgaa acccagagga
     4141 agttagtcgg atttatggat tggagttgcc tctcttacag gaggagcgga tggcctttct
     4201 aaaacgtttc tttttcgagc cagcgcttaa tatcgaagga atccagtctg gttatcaagg
     4261 tcagggtgtt aagactattt tacctgcaga agccagtgcc aagctagagg ttcgtctggt
     4321 tccgggccta gaaccgcatg atgttctgga aaaaattcgg aaacagctag acaaaaatgg
     4381 ctttgataag gtagaattat actatacctt gggagagatg agctatcgaa gcgatatgag
     4441 cgcaccagcc attctcaatg tgatcgagtt ggccaagaaa ttctatccac agggcgtttc
     4501 agtcttgccg acgacagcgg ggacaggacc tatgcatacg gtctttgatg ccctagaggt
     4561 accaatggtt gcattcggtc taggaaatgc caatagccga gaccacggtg gagatgaaaa
     4621 tgtgcgaatc gctgattatt acacccatat cgaattagta gaggagctga ttagaagcta
     4681 tgagtagaga tattatcaag ttagatcaga tcgatgtgac ttttcaccaa aagaagagaa
     4741 ccattacagc ggttaaggat gtgaccattc acatccaaga aggggatatc tacggaatcg
     4801 ttggatattc tggagcagga aaatcaaccc ttgtacgggt gattaatctc ttgcaaaaac
     4861 catctgcagg gaaaattacc attgacgacg atgtgatttt tgacggcaag gtgaccttga
     4921 cggcagagca gttgcgtcgt aaacgtcaag atatcggaat gattttccag cattttaacc
     4981 tgatgagcca aaagacagca gaggagaatg tagcctttgc ccttaaacac tctgaactca
     5041 gcaaggaaga aaagaaggct aaagtagcta agttgttgga cttggttggt ttggcagatc
     5101 gtgctgaaaa ctacccttca caactatctg gagggcaaaa acagcgtgtg gcaattgcgc
     5161 gtgccttggc caatgatcca aaaatcttga tttcagacga gtcaacttct gcccttgatc
     5221 cgaagacaac caagcagatt ttggccttgt tgcaagattt gaaccaaaaa ttaggcttga
     5281 ctgttgtctt gattacgcat gaaatgcaga ttgtcaaaga cattgccaac cgtgtggcgg
     5341 ttatgcagga tgggcatttg attgaagagg gtagtgtgct tgaaatcttc tcagacccta
     5401 aacaaccttt gactcaagac tttatctcaa cagccacagg tattgacgaa gccatggtca
     5461 aaatcgagaa gcaagaaatc gtggaacact tgtctgaaaa cagtctcttg gtgcaactca
     5521 agtacgctgg atcttcaaca gacgagccac ttttgaatga attgtacaag cattatcaag
     5581 taatggctaa tattctctat gggaatatcg aaatcctcga tggtactcct gttggagaat
     5641 tggtggtggt cttgtcaggt gaaaaagcag cgttggcagg tgcccaagaa gccattcgtc
     5701 aagcaggcgt acagttaaaa gtattgaagg gaggacagta agatggaatc attgattcaa
     5761 acctatttgc caaatgtcta taagatgggt tggtctggtc aggcaggctg gggaacagct
     5821 atctacctaa ccctctatat gacagttctt tccttcatta tcggaggctt cttggggcta
     5881 gtggcaggtc tctttctcgt cttgacagcg ccaggtggtg tcttggagaa taaagtcgta
     5941 ttctggattt tagacaaaat tacctcaatt tttcgtgcga ttccctttat catcctcttg
     6001 gcaatcttgt caccactttc tcacttgatt gttaaaacaa gtatcgggcc aaatgcagcc
     6061 cttgtcccac tttcttttgc agtctttgcc ttctttgccc gtcaggtgca ggttgtcttg
     6121 gctgaattgg atggcggtgt cattgaggcg gctcaagcga gcggagcgac tttctgggac
     6181 atcgtgggtg tttacctatc agaaggtctt ccagatttga tccgtgtgac gactgtgacc
     6241 ttgatttccc ttgttgggga aacagctatg gccggtgcgg ttggagctgg tggtatcggt
     6301 aacgtagcca tcgcttatgg atttaaccgc tacaatcacg atgtgaccat cttggcaacc
     6361 attattatca ttttgattat ctttgcaatc caattcttgg gagatttctt gactaagaaa
     6421 ttgagtcata aataaaaaga gccgcgtggc tctttttaac tgatcagatt ttctgtgcaa
     6481 attttttact caaggcttgt ccaatcaagg cacccactag ggctccgatg acaatacttg
     6541 cgataaatag aaggacagtt ccagggtttg gagcgaccat gatacggtcg atatattctt
     6601 gggattttcc tcttgccaga agagtagcca tataggcttt gggcgcaatc cacataagca
     6661 agattggtcc tgttgtacta aaggcgaaaa taatgaaaga aaggaagttc tttgttttgt
     6721 ccttgtattt tcctaaatga gctacttcat ctgctaggag gccacagata attccaggaa
     6781 ggaaggaacc agcaccgtgt ttagttccca agaaaaagag ggcaatgaca aggccgatag
     6841 tggtaatggc tccaaagcgc ggaacttttg cgactaggat catatagacg ctaccgccga
     6901 caagggcagt aaaggcaggc gcataaaaca tgtttccagt ttggtcaaag agattgccca
     6961 aaaggacacc aatccccatg cagaggaagt aaagaactgc aaaaagcagt gtagttaaga
     7021 tagatttctt catgaattgt ctcctgataa ttttttcaca attctcattg taccacggtt
     7081 tggtgggatt gtaaatggat actagaagtt tttgaaaacg cttgaaatat gatataatgg
     7141 tttcatagtt atttttaggg gaatatcatg ggtagatttt tagactttgt ctttaatcgt
     7201 ttctttttag ggatgattgc gacagccttc ttttggctat taactttagc aggagggatt
     7261 atccttggtc tagcgccggc tagtgccacc ttgatgagct tatatgcaga acatggttat
     7321 agctttcggg aatacagttt gaaggaggct tggtctcttt acaagcaaaa ttttgtctca
     7381 agcaacctga ttttctatag ctttttaggt gtgggtctag ttttgaccta tggtttgtat
     7441 ctcttggtgc aattgcctca tcagaccatt gttcatttga ttgcgaccct tttgaatgtc
     7501 ctagtagttg ccctgatctt tttggcttat acagtatctt taaaattaca agtttatttt
     7561 gccttgtcct atcgaaatag tctcaaatta tccttgattg gcatctttat gagtctagca
     7621 gctgtggcca agattctcct tgggactgtg ctacttgtag caattggtta ttatatgcct
     7681 gccctgctat tttttgtagg aattgggatg tggcatttct ttatcagtga tatgttggaa
     7741 cctgtctatg aaatcatcca tgaaaaattg gcgacaaaat agaatgaagc agttttggct
     7801 acatacgctt ctaagaacct atagttcagt gatgatcatt atcattgcga gttttgcaat
     7861 cttactctct tacgctgact gggattcacg tgaaaaggaa gcccagagag tagcccagcg
     7921 tgtaactgct agaacagtga gtgaaattga atattaccat agagagtcaa cccagatagc
     7981 tcaggcttta gttgaaaacc aagctcgtat tgagggaatc tataaatact ttagtcttag
     8041 catgccagac tatttttact ggcaattaga gcggaaagct tcgccttata tatcagtctc
     8101 tctgtatgaa aatgttgatg acctctatgt tcgaaatgat tttgtaactg gagtggccat
     8161 tgcttttcaa gattacaagg aagtctatgt ttctactaaa gacaaacgta gtggagaaaa
     8221 aatcagggct gaggatttca aaccagcagg aaatagtttt gccattccgg tgtcagatcc
     8281 agtgtcagat caagatttag gagtgattta catctccttg gatcctgctg ttttatacca
     8341 tgccattgat aatactagag gtcatactcc gatggcagta acagtgacct caccttttga
     8401 tacggagatt tttcatattg gtgagacagt tgataaggag agtgaaaatt ggctagttgg
     8461 cttaacttct catggatatc aggttcaggt ggcagttcct aaaaactttg ttttacaagg
     8521 aacagtgact agctctgctt tgattgtggg tttgagcctt ctctttattg tcattcttta
     8581 tctgactttg aggcagactt ttgctaatta ccaaaagcag gtagtggatt tagtagaatc
     8641 cattcaagtc attgctcaag gcgaagaggg gcgtcggatt gacatttccg agaaagatca
     8701 ggaattactc ctaatcgcgg agacgaccaa tgatatgttg gatcgattgg aaaagaatat
     8761 ccatgatatt taccagttag agcttagtca aaaagatgcc aatatgcgag ccttgcaggc
     8821 gcaaatcaat cctcatttta tgtataatac gctggagttc ttgcgcatgt atgcagttat
     8881 gcagagtcaa gatgagttgg cagatatcat ttatgaattc agtagtctct tgcgtaacaa
     8941 tatttccgac gaaagagaga ccctcctcaa acaggaatta gaattttgcc gtaaatacag
     9001 ctatctctgc atggttcgct atcccaagtc cattgcctat ggtttcaaga tagatccaga
     9061 gttagagaat atgaagattc ccaagtttac cttgcaaccg ctggtagaaa actatttcgc
     9121 gcatggtgtt gaccacaggc ggacagataa tgtgattagc atcaaggctc ttaaacagga
     9181 tggttttgtg gaaattttgg tggtcgataa tggtagagga atgtcggctg aaaagttggc
     9241 aaatatccga gaaaaattaa gtcagagata ttttgaacac caagccagct acagtgatca
     9301 aaggcagtct atcgggattg tcaatgtaca cgagcgtttt gtgctctatt ttggagaccg
     9361 ctatgccatt actatagagt ctgcagagca agccggtgtt cagtatcgta ttacaattca
     9421 agatgagtag aaagggagaa aatgtataaa gtattattag tagatgatga gtacatggtg
     9481 acagaaggtc tgaagcgttt gattcccttt gataagtggg atatggaggt tgtcgcaaca
     9541 gccagtcatg ccgatgaagc tctagaatat gttcaggaaa atcctgtcga tgtcatcatt
     9601 tccgatgtca atatgccaga caaaacaggg cttgatatga ttcgggagat gaaagagatc
     9661 ttaccagatg ctgcctatat cctgctctca ggttatcagg agtttgatta tgtaaaaaga
     9721 gcaatgaacc ttagtgtggt ggactatttg gtcaagcctg ttgataaggt agagctggga
     9781 aatctgctgg agaagattgc aggtcagctc ggcgagagag ggaagaaaag tcagactctt
     9841 agtcaagaat tagacgaggc tggatttgtt agttatttag gggataagga gaattggtgg
     9901 ataggtctat ccaaggaaaa acaaggttcc ttcaccattc cctactatgt cttgggtcaa
     9961 gactggcaga ttttcatttc tggccaaccc ttagatggct tagtcgttac tccctttgaa
    10021 gctccctatc aagaacactt tgaacgctgg aagctgaatg ctgagaaaac cctcttttac
    10081 ggttctgtaa atctgcagca gtctgagagt ctctttgcct attacgaacc gatttatagg
    10141 gttatcattc agggaaatct caatcaaatc gtagaagagt taaatctctt ggagaaggta
    10201 gttcttgaaa atacgccgcg tgttccgatt actaaacagc tttttattca gtttgtcatg
    10261 gatgttttcc atttatttga acatctcaaa gctgatgacc tgacagacat cgttaaaacc
    10321 attcatgcta ttcaatcctt cgatgaattg gttccttata tcaaggaaac tctgaccagc
    10381 tttttcggtc aataccgtat gaatgaaaat gtggtcagtg tgctggaagt cattggtcgt
    10441 gattaccaaa aagagctttc cctcaaggat atcagtaagg ctctctttat caatcctgtc
    10501 tatctagggc agttgattaa gcgtgaaacc gattcgactt ttgcagagtt actaaacaaa
    10561 caacgtatta aggctgccca gcagctcttg ctttcaacta gtgacagcat cgaagatatt
    10621 tgttatgctg ttggttacag taaccttgga tatttctata aagttttccg aaaattgtgc
    10681 ggaaaatcgc caaaagccta ccgaaaacag gtagaaacta tactataaga tttgtattcc
    10741 tttacaaaat gtgctataat attatcagga ggttctatca tgaaaaagaa accaatttat
    10801 ctatgggtct tgctaatctt gtctgccctt atttcagttc cgtctctgtt tggaattgtg
    10861 agtcccttgc ctagtaaaga agcccttcgt gctgctcaga aacaagttgc aggggtcaat
    10921 gctcagcaat tagaagacca gcttaattat acctatagag tagcagaagc atctcattct
    10981 atttttaatg ttgctttgat tgtgctatct actattttag tggtcgtagc gattgtcttc
    11041 cttgttcgta aaaatttgca atatgcaaac tatacttacg ttggctatgt tttgttagct
    11101 attattggtt cgatttatgg ctatgttggt ttacaagatg ctgtgcagtt ggttcaagat
    11161 gagagcatgc gtttgacagt gagtattggt tcaaaagctg ttagcatttt ctatatcgtc
    11221 atcaatgttc tcttcctagc ccttgtcttc tataagatgt ggcgacaaca gaaagccttg
    11281 gcagaagaag aggaaacaga agagcttgcc taagtattga caaaaaagaa ctatcaagat
    11341 acaatcttgg tagttgtttt ttgattatag ataaaatcat ggaggtacat atgaagacaa
    11401 tttctttagt ttatattagt ttgagtggca acactgaaag ttttgtgacg cgcttgaaag
    11461 actatctctt gtcccagtac aaagggattg aggttcaaaa gattcatatc aaggatttgg
    11521 tcaaggaagg caaaaatttc tacgaaatgg accatcccta tgttgctttt ttgccgacct
    11581 atctcgaagg tgggaatggc gtggataacg gagatgttga gattttgacg acaccggtgg
    11641 gagattttat tgcctatggt aacaatgcta gtaagtgttt tggtgtggtt gggtcaggaa
    11701 atcgtaactt taataaccaa tactgcctaa cagccaagca atacagtcaa cgctttggct
    11761 ttcctgtatt ggctgacttt gaaatgcgag gcatgctgga agatatcaaa catgtcgcag
    11821 ctattatcgc agatttgtat gagttggaaa aggagaatta atactcaatg aaaatcaaag
    11881 agcaaactag gaagctagtc gcaggttgct caaagcacag ctttgaggtt gcagatagag
    11941 ctgacgtggt ttgaagagat tctcgaatag tataaagttc ttataagagg tggttgaagt
    12001 tgttcaactg ttttttgagt ataaacagtc tttaagaacg taaaaaagca taagttcagg
    12061 tcttcaaaac cttgttctta tgctttttat catagaaata tttgctgaat tttctcttga
    12121 aatgaaacct tatcttcatc tcgcgcttgt cccttaccct gcttttccga ttgcgagtgc
    12181 gtagataaag aagatggcga agccaaagag gaaaatcaat cccgcaatgg caaggtgttt
    12241 gagccaagaa ggaagatttt tgttttcacg tgttaccaag gcgtaattca aaagagcaaa
    12301 gaaaggtgtt gtcaggaaag agccaatcat ggcaaagcgg agcatggttg aaacctgacc
    12361 agcgaagaac ttgataatga tgataccgat gatagcagtg atggtcatcc agatgttcaa
    12421 agatttacga ttatcctctt tttgactgat tagcagtcgg agagattcct gattaacgcg
    12481 agaatagcca tcgataacag ttataactgt tccaaagata cagaggaagg caataaaggt
    12541 aatcaagtaa cgggaccatt cgccaagaac agaggcatac atgcccacga attgagagat
    12601 gtatttggct gaagcagctt caaccgcctg ccctgtagga tactgaatca gtgctcccag
    12661 tgccacaaag aagacggcta ggatagctgt tccaatataa ccagtgttaa agtcaaacag
    12721 agcgtcttct gtgttaaagt tgacggtctt tctcttttca gctgaccaaa gtgaattgat
    12781 ggctgaaatt tcaataggag ccggcatcca tcctaggagg gagacgatga agggcagagc
    12841 tgccatttgc caaggtgtct tctcgacaaa atcagaactg tattctggat gcttgaccgc
    12901 cgcaatgata actgcaagaa cagtcgcaat ggttaaggca gacataatcc atttgaccat
    12961 gccgtctaaa agtttgtagc ctccaaagag tagcatagcc caaatgattg caacgagaat
    13021 gagggaccac tgagtaatgc taagtccaat cattgggaag gcactggcga tgatagctga
    13081 gcacagaatg gcaacaccag ccgtgttgac catagccgaa aagacattga ggataaagaa
    13141 aatccagaga tagagttttc ctttttcggc ataaccttca accaaagtct ttccagtatc
    13201 agctgtgtat tcagcaccaa aacggaaaaa tggatattta aagacattgg ctaagatgac
    13261 caagagaagt agagaccaac cgtaagaacc gccagcttga gttgaggata caatgtggga
    13321 acctccaaca gcggcagttg ccattaggat tccaggtccc attgctttta gcttgcttgc
    13381 ccaggttgat tgatttaagg aaatagcttg cgacatgata aatactcctt tttgaatttt
    13441 cagaataatc tgtatatgta atctatattc tacaaaaaat tttggaaaaa tgcaagagta
    13501 ttttgaaaaa agatagaaaa tttacaaaga aaatctgaaa taactgttat gatataga
//
LOCUS       DAVLYR010000040        13310 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLYR010000040 DAVLYR010000000
VERSION     DAVLYR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.040"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            5..1315
                     /locus_tag="VYO63_000921"
     CDS             5..1315
                     /locus_tag="VYO63_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1671871.1"
                     /translation="MLVSSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGAL
                     DTYHPIAYRFAFIVVMVSILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFV
                     SLGVFLLTYPFYFIRLFERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYL
                     ATKPKKLQATAVLVSFIAANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFK
                     EKLAIFVGSPILMLAMGVLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFN
                     SSLPYRDFRNFTFGPVLDYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLV
                     LNKEYLKGHGIGSSYIMELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSL
                     LILNNLFFMPRSSFSESFFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            1344..2378
                     /locus_tag="VYO63_000922"
     CDS             1344..2378
                     /locus_tag="VYO63_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1671872.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            2578..3315
                     /gene="mecA"
                     /locus_tag="VYO63_000923"
     CDS             2578..3315
                     /gene="mecA"
                     /locus_tag="VYO63_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1671873.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            3466..4752
                     /locus_tag="VYO63_000924"
     CDS             3466..4752
                     /locus_tag="VYO63_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1671874.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            4754..5623
                     /locus_tag="VYO63_000925"
     CDS             4754..5623
                     /locus_tag="VYO63_000925"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1671875.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAAAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            5699..5770
                     /locus_tag="VYO63_000926"
                     /pseudo
     CDS             5699..5770
                     /locus_tag="VYO63_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5918..6856
                     /gene="msrB"
                     /locus_tag="VYO63_000927"
     CDS             5918..6856
                     /gene="msrB"
                     /locus_tag="VYO63_000927"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1671876.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            6914..8638
                     /locus_tag="VYO63_000928"
     CDS             6914..8638
                     /locus_tag="VYO63_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671877.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            8640..10388
                     /locus_tag="VYO63_000929"
     CDS             8640..10388
                     /locus_tag="VYO63_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671878.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLVIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            10780..12039
                     /locus_tag="VYO63_000930"
     CDS             10780..12039
                     /locus_tag="VYO63_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1671879.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKCPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    12115..12250
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            12289..12789
                     /locus_tag="VYO63_000931"
     CDS             12289..12789
                     /locus_tag="VYO63_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1671880.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            12865..13233
                     /gene="rplL"
                     /locus_tag="VYO63_000932"
     CDS             12865..13233
                     /gene="rplL"
                     /locus_tag="VYO63_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1671881.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
BASE COUNT         3943 a         2524 c         2900 g         3943 t
ORIGIN      
        1 gttcttgttg gttagtagct taattgcttg ttggaatgat atcaagtatt acttaatcca
       61 ttttattttc tatttaacca tttttgtatt tctggtatca agaccgacca ttgattattt
      121 tagggatggt gctttggata cctatcatcc aatagcctat cgttttgcct ttatagttgt
      181 catggtttcg attctgggct tgaccacagg aggcattctg gctcgttact tcatagctag
      241 gaagaaaata aaagtagcaa atataggaaa ttctctaaaa gaggtttata tcaagcggtt
      301 acgctttgta tcactaggag tttttcttct aacctatcct ttctatttca ttcggttatt
      361 tgaacggctc ttgtatcgtt tgcagacttc ctactatgcc tactatgcaa attttgaaag
      421 taaactgcct tattttacct acattttgtc tacctttacg gtctatgcaa tgtgtatgta
      481 tctggcaacc aagccaaaga aattgcaggc cacagcagtg cttgtctcct ttattgcagc
      541 taatactatt catttggcaa ttgggacacg aaatcccttt attttaagta ttttatttgc
      601 ttttgtttat tactttatgc gggagcaaac tgaaaaagga aaatggattg ggtttaaaga
      661 aaagttagcg atttttgtag gttctcctat tctcatgtta gcgatgggag tactcaatta
      721 tgtacgggat aatgtccaag tttctcatac aggtttctgg gatatcttac ttgactttat
      781 ctataaacaa gggactagtt ttggtgttct ggctcgaggt tttctattta acagtagcct
      841 cccttaccga gatttccgta attttacttt tggtcctgtt cttgattatt ttgcaagggg
      901 gagtttggga gccattttcg gaggaaaagc ctttgaacat acaaccaata gtgtggaact
      961 agctattgat agtaatagtt atgcccacaa tctatcctat cttgtcttga acaaggaata
     1021 cttgaaaggg catggtatcg gaagtagtta tatcatggag ttgtataccg actatggtat
     1081 gattggagtc tttctgctta gtttcttact cggcgtatta tttatagcca tgctgcaagt
     1141 agcctatcgc tcaaggacaa tcctatttgc tttatcccta ctcatcttga ataatctatt
     1201 ctttatgcca agaagcagct tttcagaaag tttcttcaat ttatttacaa tgcaattctg
     1261 gggaattgtt cttgtgatta tatttgtagc aaaaatgctt acaaaggaaa actagtatct
     1321 actaaacaaa ggagaaaaaa atcatgtttg aacattattc agtagctgat ttgtttgcaa
     1381 atctttataa aaaacgaaaa gcaaatattt tagctctcat cgctttattt gctctcattg
     1441 ctgtaccatt tacaattaaa gcagttagga ataaaaacac tgtcaaagat acaacaagtt
     1501 attcaactta tcttatctat aaaatcactc ctccagaaga ttcggccaaa acgattttga
     1561 atcatcaaat tggtggttat agtgattttt atgggaaatt gattgatggt aatttgaatg
     1621 gagcttatct tttcaatgat gtagaaccca gtgagttgaa aaaaattgcc agtgaattag
     1681 acacgacgga aacaaccttg aaaaattcaa cgaatgacta ttggtggaaa aaattgaccg
     1741 tctactatat gattgacgat gcaggggttg gtgtgaaaat tttgacatca agtaaagatg
     1801 ccaatgactt gttagagaaa aaaattgatg ggttgattga gaaatttaaa catgcttatg
     1861 caaatgtgaa aattgaaaaa ctggaaacca tcaactctaa agaattgaac gcaaatggtg
     1921 aaacagcgct tggcttaaat gtgaaaaatc tgattcttcg tttagttgtt attggagtgg
     1981 tttgtgtgat tttggttgtg atgggaaatg tgttagttta tctctttaat ccaacaatca
     2041 atagagtagg tgatttttct cagtatcaaa ttgattttgt aacagagatt acaacaattg
     2101 ctaacctagc agatgttttg tcatacaaaa atactggaca ggaattgacc atcgttagct
     2161 caaataaagc tatcctagat aaattgaaac agagtcaaga agctttaaaa ggaatgcatt
     2221 ttgtagattt gcaggatgta tcatctcttt tggaaagaga tacagtcctt cttgttgaag
     2281 agtacggagt gactcgttat aagaaatttg agcaaagtct tcaaattctc agaaacttaa
     2341 atcgttctat cctcggtgta gcaaccttta aattataagt attctgatgt ataaggtctt
     2401 caaaatcggc taagagccga ttttgaaggc tttttctatc tgtctgcaga aattttctct
     2461 tggagtctcc tatagaaaga ccctagggaa aatctatccg tatagaggag ccttcttttg
     2521 ataaaatcgt aaaaatctag tataatagat agaactgaaa gtatgaggtt actagcaatg
     2581 aaaatgaaac aaattagtga tacaacttta aaaatcacga tgtctttaga ggatttgatg
     2641 gatcgtggaa tggagattgc tgactttctc gttcctcaag aaaaaacaga agagttcttt
     2701 tatgctatct tggatgagct agagatgcct gatagctttc tggatacagg catgttgagc
     2761 ttccgtgtga ctccaaaacc tgataaggta gatgtctttg tgaccaagtc aaagattgac
     2821 caaaatctag attttgaaga cttatcggat ttgccagata tggaagaatt ggctcaaatg
     2881 tctccagatg aatttatcaa aaccctggaa aaaagcatcg cagacaaaac caaggatgat
     2941 atcgaagcca ttcaatctct tgagcaagtt gaagccaagg aagaagagca agagcaggct
     3001 gaacaagaag ctgagagtaa gaaagaacct tacatctact acatcctttc ttttgctaag
     3061 ttggctgact tggtagcttt tgccaagaca gtgacttttg aaatggaaac ttctgaactc
     3121 tacaaaatga acgagcgcta ttatttgacc attttagtgg atattgaaaa tcatccaagc
     3181 ccatatccag cttggctgtt ggcccgtatg cgcgagtttg cagacgatag tgatatcagt
     3241 cgctcagtct tacaagagta tggtcaagtc ttgatgagtc acgatgcagt gctcaatctg
     3301 caaaaaatcg gctaatcctt tctggaaagc tattttcaga ttttaagaag agcgaatcgt
     3361 ctgattcgtt ttttcttttt atactgaaat agtgatttac tataatagga attttcacaa
     3421 aattctgtta taatggctat attagaaaat ttcgaggaga taaagatgac agttaaaatt
     3481 gctttactag gatttggtac cgttgcaagt ggtgtgcctt tcctcctaaa ggaaaatgga
     3541 ggaaaaatca atcaatcagc acattcagat atcaaagttg ctaaggtatt ggtcaaggat
     3601 gaagatgaaa aaaatcgctt gcttgcagca gggaatgact ttaactttgt aaccaatgtg
     3661 gatgatattt tatcagacca ggatattact atcgtagtgg aattgatggg gcgtattgag
     3721 cctgctaaaa cctttatcac tcgtgccttg gaagctggaa aacacgttgt tactgctaac
     3781 aaggaccttt tagctgtcca tggcgcagaa ttgctagaaa tcgctcaagc taacaaggta
     3841 gcactttact acgaagcagc agttgctggt gggattccaa ttcttcgtac tttagcaaat
     3901 tccttggctt ctgataaaat tacgcgcgtg cttggagtag tcaacggaac ttccaacttc
     3961 atggtgacca agatggtgga agaaggctgg tcttacgatg atgctcttgc ggaagcacaa
     4021 cgtctaggat ttgcagaaag cgatccgacg aatgacgtag atgggattga tgcagcctac
     4081 aagatggtta ttttgagcca atttgccttt ggcatgaaga ttgcctttga tgatgtagcc
     4141 cacaagggaa tccgcaatat cacaccagaa gacgtagctg tagctcaaga gcttggttac
     4201 gtagtgaaat tggttggttc tattgaggaa acttcttcag gtattgctgc agaagtgact
     4261 ccaaccttcc taccgaaagc gcacccactt gctagtgtga atggcgtaat gaacgctgtc
     4321 tttgtagaat ctatcggtat tggtgagtct atgtactacg gaccaggtgc gggtcaaaaa
     4381 ccaactgcaa caagtgttgt agctgatatt gtccgtatcg ttcgtcgttt gaatgatggt
     4441 accattggca aagacttcaa cgaatatagc cgtgacttgg tcttggcaaa tcctgaagat
     4501 gtcaaagcaa actactattt ctcaatcttg gctccagact caaaaggtca ggtcttgaag
     4561 ttggctgaaa tcttcaatgc tcaagatatt tcctttaagc aaatccttca agatggcaaa
     4621 gagggtgaca aggcgcgtgt cgttatcatc acacacaaga ttaataaagc ccagcttgaa
     4681 aatgtctcag ctgaattgaa gaaggtttca gaattcgacc tcttgaatac cttcaaggtg
     4741 ctaggagaat aagatgaaga ttattgtacc tgcaaccagt gccaatatcg ggccaggttt
     4801 tgactcggtc ggtgtagctg taaccaagta tcttcaaatt gaggtctgcg aagaacgaga
     4861 tgagtggctg attgaacacc agattggcaa atggattcca catgatgagc gtaatctctt
     4921 gctcaaaatc gctttgcaaa ttgtaccaga cttgcaacca agacgcttga aaatgaccag
     4981 tgatgtccct ttggcgcgcg gtttgggttc ttccagctcg gttatcgttg ctgggattga
     5041 actagccaac caactgggtc aactcaactt atcagaccat gaaaaattgc agttagcgac
     5101 caagattgaa gggcatcctg acaatgtggc tccagccatt tatggtaatc tcgttattgc
     5161 aagttctgtt gaagggcaag tctctgctat cgtagcagac tttccagagt gtgattttct
     5221 agcttacatt ccaaactatg aattacgtac tcgcgacagc cgtagtgtct tgcctaaaaa
     5281 attgtcttat aaggaagctg ctgctgcaag ttctatcgcc aatgtagcgg ttgctgcctt
     5341 gttggcagga gacatggtga ccgctgggca agcaatcgag ggagacctct tccatgagcg
     5401 ctatcgtcag gacttggtaa gagaatttgc gatgattaag caagtgacca aagaaaatgg
     5461 ggcctatgca acctaccttt ctggtgctgg gccgacagtt atggttctgg cttctcatga
     5521 caagatgcca acaattaagg cagaattgga aaagcaacct ttcaaaggaa aactgcatga
     5581 cttgagagtt gatacccaag gtgtccgtgt agaagcaaaa taaagaatag aagataggat
     5641 ggggaaactc ttgaccagag ggtttcatat cctttttgtg aaaagaagtt tatactcaat
     5701 gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc tcaaaacagt gttttgaggt
     5761 tgtggataga actgaagaag tcagctcaag acactgtttt gaggttgcag atagaactga
     5821 cgaagtcagt aacatatata cggtaaggtg acgctgacgt ggtttgaaga gattttcgaa
     5881 gagtattagt taaaaacgtg ataaaggaga aataaagatg gcagaaattt atctagcagg
     5941 tggttgtttt tggggcctag aggaatattt ttcacgcatt tctggagtgc tagaaaccag
     6001 tgttggctac gctaatggtc aagtcgaaac gaccaattac cagttgatca aggaaacaga
     6061 ccatgcagaa acggtccaag tgatttacga tgagaaggaa gtgtcactca gagagatttt
     6121 actttattat ttccgagtta tcgatcctct atctatcaat caacaaggga atgaccgtgg
     6181 tcgccaatat cgaactggga tttattatca ggatgaagca gatttgccag ctatctacac
     6241 agtggtacag gagcaggaac gcatgctggg tcgaaagatt gcagtagaag tggagcaatt
     6301 acgccactac attctggctg aagactacca ccaagactat ctcaggaaga atccttcagg
     6361 ttactgtcat atcgatgtga ccgatgctga taagccattg attgatgcag caaactatga
     6421 aaagcctagt caagaggtgt tgaaggccag tctatctgaa gagtcttatc gtgtcacaca
     6481 agaagctgct acagaggctc catttaccaa tgcctatgac caaacctttg aagaggggat
     6541 ttatgtagat attacgacag gtgagccact cttttttgcc aaggataagt ttgcttcagg
     6601 ttgtggttgg ccaagtttta gccgtccgat ttccaaagag ttgattcatt attacaagga
     6661 tctgagccat ggaatggagc gaattgaagt tcgttctcgt tcaggcagtg ctcacttggg
     6721 tcatgttttc acagatggac cgcgggagtt aggcggcctc cgttactgta tcaattctgc
     6781 ttctttacgc tttgtggcca aggatgagat ggaaaaagca ggatatggct atctattgcc
     6841 ttacttaaac aaataaaaca gagagtgggg cttcccactt tcttcatttc tagaatatga
     6901 atagaaggga tttatgaaac acctattatc ttacttcaaa ccctacatca aggaatcaat
     6961 tttagccccc ttgttcaagc tgttagaagc tgtttttgag ctcttggttc ccatggtgat
     7021 tgctgggatt gttgaccaat ctttacctca gggagatcaa ggtcatctct ggatgcagat
     7081 tggcctgctc cttatctttg cagtaattgg cgttttagtg gccttgatag ctcaatttta
     7141 ctcagcaaag gcagcagtag gctttgctaa ggaattgaca aacgatcttt atcgtcatat
     7201 tctttccttg tccaaggaca gcagagaccg tctgacaact tctagtttgg tcactcgctt
     7261 gacttcggat acctaccaga ttcagactgg tatcaatcaa ttcctgcgtc tctttttgcg
     7321 agcgcccatt atcgtttttg gtgccatttt tatggcttat cgaatctcag ctgagttgac
     7381 tttctggttc ttagtcatgg ttgccatttt gaccattgtc attgtagggc tatctcgatt
     7441 ggtcaatcct ctctacagta gtctcagaaa gaaaacggac caactggttc aggaaacgcg
     7501 ccagcaattg caagggatgc gggttattcg tgcttttggt caagaaaaac gagagttaca
     7561 gatttttcaa acccttaacc aagtttatgc tagattacaa gaaaagacag gtttctggtc
     7621 tagtttatta acacctctga cctatctgat tgtcaatgga actcttctcg ttattatctg
     7681 gcaaggctat atttcaattc aaggaggagt gctcagtcaa ggtgctctca ttgctcttat
     7741 caattacctc ttacagattt tggtggaatt ggtcaagctt gccatgctga tcaattccct
     7801 caaccagtcc tatatctcag ccaagagaat cgaggaggtc tttgcggaat ctcccgaaaa
     7861 catccattca gaattagaac aaaagcaagt taccagtggt cgggttttac aagtccaaga
     7921 attgactttt acctatcctg atgcggccca gccttctctg agagacattt cctttgatat
     7981 gactcaagga caaatccttg gtatcattgg ggggactggt tctggtaaat caagcttggt
     8041 gcaactctta cttggacttt atccagtaga caaggggaac attgaccttt atcaaaatgg
     8101 acgtagtcct cttaatttgg agcagtggcg gtcttggatt gcctatgtac ctcaaaaggt
     8161 cgaactcttt aaaggaacca ttcgttccaa cttgactcta ggtttcaatc aagaagtatc
     8221 tgaccagaaa ctctggcagg ccttggagat tgcgcaagct aaggattttg tcagtgataa
     8281 ggaaggactt ttggatgccc tagttgaggc aggggggcga aatttctcag gtggacaaaa
     8341 acaaaggttg tctatcgccc gagcagtctt gcgccaagct ccgtttatca tcctagatga
     8401 tgcaacctcg gcactggata ccattacaga gtccaagctc ttgaaagcta ttagagaaaa
     8461 ttttccaaac acgagcttaa ttttgatctc tcaacgaacc tcaactttac agatggcgga
     8521 ccagattctc ctcttggaaa aaggtgagtt gctagctgtt ggcaagcacg atgacttgat
     8581 gaaatccagc caagtctatc gtgaaatcaa tgcatcccaa catggaaagg aggactagaa
     8641 tgaaacgaca aactgtaaac cagacgctca aacgtttagc cgtagattta gcaaaccatc
     8701 ccttcctcct tttcctagcc tttctaggaa ctattgccca agttggctta tcaatttacc
     8761 tacctattct gattgggcag gtcattgacc aagtcctagt ggctggttca tcaccagttt
     8821 tttggcagat ttttctccag atgctcttgg tggtaatagg aaatactctg gtacaatggg
     8881 ccaatcctct cctctataat cgtctaatct tctcttatac tagagatttg cgagagcgaa
     8941 tcatccataa gctccatcgt ttaccgattg cttttgtaga taggcaaggt agtggagaga
     9001 tggttagtcg tgtaaccacg gacatcgaac agttggcagc tggcttgacc atgattttta
     9061 accaattttt cattggtgtt ttgatgattt tggttagtat tctagccatg ctccaaattc
     9121 atctcctcat gactctctta gtcttgctgt tgacgccact gtccatggtg atttcacgct
     9181 ttattgccaa gagatcctat catctcttcc agaagcaaac agagacgagg ggaattcaga
     9241 ctcagttgat tgaagaatcg cttagccagc agactataat ccagtccttc aatgctcaaa
     9301 cagaatttat ccaaagattg cgtgaggctc atgacaacta ctcaggctat tctcagtcag
     9361 ccatctttta ttcttcaacg gtcaatcctt cgactcgctt tgtaaatgca ctcatttatg
     9421 cccttttagc tggagtagga gcttatcgta tcatgatggg ttcaaccttg accgtcggtc
     9481 gtttagtgac ttttttgaac tatgttcagc aatacaccaa gccctttaat gatatttctt
     9541 cagtgctagc tgagttgcaa agtgctctgg cttgcgtaga gcgtatctat ggagtcttag
     9601 atagccctga agtggctgaa acaggtaagg aagtcttgac cagtgaccaa gttaagggag
     9661 ctatttcctt taaacatgtc tcttttggct accatcctga aaaaattttg attaaggact
     9721 tgtctatcga tattccagct ggtagtaaga tagccatcgt tggtccgaca ggtgctggaa
     9781 aatcaactct tatcaatctc cttatgcgtt tttatcccat tagctcggga gatatcttgc
     9841 tggatgggca atccatttat gattatacac gagtatcatt gagacagcag tttggtatgg
     9901 tgcttcaaga aacctggctc acacaaggga ccattcatga taatattgcc tttggcaatc
     9961 ctgaagccag tcgagagcaa gtaattgctg ctgccaaagc agctaatgca gactttttca
    10021 tccaacagtt gccacaggga tacgatacca agttggaaaa tgctggagaa tctctctctg
    10081 tcggccaagc tcagctcttg accatagccc gagtctttct ggttattcca aagattctta
    10141 tcttagacga ggcaacttct tccattgata cacggacaga agtgctggta caggatgcct
    10201 ttgcaaaact catgaagggc cgcacaagct tcatcattgc tcaccgtttg tcaaccattc
    10261 aggatgcgga tttaattctt gtcttagtag atggtgatat tgttgaatat ggtaaccatc
    10321 aagaactcat ggatagaaag ggtaagtatt accaaatgca aaaagctgcg gcttttagtt
    10381 ctgaataagc cattctcttt tgaaagttta tggacgaaaa aagttgcctt cgagtgactt
    10441 ttttgttaca atagctagaa aaattgttca ctgtaatact caatgaaaat caaagagcaa
    10501 actaggaagc tagccgtagg ttgctcaaag cacagctttg aggttgtaga taagactgac
    10561 gaagtcagct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagctcaaaa
    10621 cactgttttg aggttgcaga tagaactgac gaagtcagct caaaacactg ttttgaggtt
    10681 gcagatagaa ctgacgaagt cagtaaccac acctacggca aagtgaatct gaagtggttt
    10741 gaagagagta caacttgtct tttagaaaag gagcctataa tgaaagtctt tcagcatgta
    10801 aatatcgtga cttgtgatca aaatttccat gtttatcttg atggaatctt agcagtcaag
    10861 gattctcaaa tcgtctatgt cggtcaagat aagccagcgt ttttagaaca agctgagcag
    10921 attatagact atcagggagc ttggattatg cctggtttgg tcaattgtca cacccattct
    10981 gcaatgacag gtctgagagg gatccgagat gacagcaatc tccatgaatg gctcaatgac
    11041 tatatctggc cagcagaatc tgagtttact cccgacatga ctaccaatgc ggttaaagaa
    11101 gccctaacag agatgctcca gtcaggaaca acaaccttta acgatatgta taatcccaat
    11161 ggtgtggata tccagcaaat ttatcaggtg gtgaaaactt ccaagatgcg ttgttatttt
    11221 tctccgactc tcttttcttc agagacagaa acaactgctg agactataag cagaactcga
    11281 tccatcatag atgaaatctt aaaatataaa aatccaaatt tcaaggttat ggtagcacct
    11341 cattctccgt atagctgcag tagagacttg ctggaagcga gtttggaaat ggcaaaagag
    11401 ctaaatattc cgctccatgt ccatgtggcg gagaccaagg aggagtcagg aattatcctc
    11461 aaacgatacg gcaaatgccc ccttgctttt cttgaagaac tgggttattt agatcatccg
    11521 tccgtatttg ctcacggggt cgaattaaac gagagagaaa ttgaacgctt ggcatcttct
    11581 caagtggcta tcgcccacaa tcctatcagt aacctcaaac tggcatcagg aattgctcca
    11641 attatccagc tccaaaaagc gggagtagca gtcggaattg cgactgactc ggttgcttcc
    11701 aataacaatc tagatatgtt tgaggaagga aggactgcag ctcttcttca gaagatgaaa
    11761 agtggggatg ccagccagtt tccaatcgaa acagctctca aggtactgac aatcgaaggg
    11821 gctaaggtcc ttggaatgga aaatcagata ggaagtctgg aagtcggcaa gcaagcagat
    11881 tttctggtca ttcaaccaca agggaaaatt catctccaac ctcaggaaaa tatgctgtct
    11941 cacctggttt atgcagttaa atctagtgat gtagatgatg tttatatcgc cggagaacag
    12001 gttgttaagc aaggtcaagt cctgacagta gaactttaaa agaaaaatta cgaaaaattt
    12061 taaaaaaagt ttgcaaaaat cttgcattct ttttttgact atgctatact tatatacggt
    12121 ttaaaaaaac tgcctaagac agtaggggag ctcgactcat aaatatccta ccgaggacaa
    12181 aacgtatcat gtaaaaagaa gcgtattgta ctttcgtgtc taggtttggg cgcgtttttc
    12241 tttttgaaaa attccccaag caaaataatt acggaggtga acacactaat gagtgaagca
    12301 attattgcta aaaaagcgga actagttgac gtagtagctg aaaaaatgaa agctgctgca
    12361 tctatcgtcg ttgtagacgc tcgtggtttg acagttgagc aagatacagt tcttcgtcgt
    12421 gagcttcgtg gaagcgaagt tgagtataaa gttattaaaa actcaatctt gcgtcgtgca
    12481 gctgaaaaag ctggtcttga agatcttgca tctgtatttg ttggaccatc tgcagtagca
    12541 ttttctaatg aagatgttat cgcaccagcg aaaatcttga acgacttttc taaaaacgct
    12601 gaagcacttg aaattaaagg tggtgcaatc gaaggcgctg tcgcatctaa agaagagatt
    12661 cttgcacttg caactcttcc aaaccgcgaa ggacttcttt ctatgctcct ttctgtactt
    12721 caagcgccag tgcgcaacgt tgctcttgca gtcaaagcgg ttgcagaaag caaagaagac
    12781 gcggcttaat cttaagctac acagcgtagc ctagctacga aaaaaactat tataaaattt
    12841 aaaacttatt tggaggaaat aacaatggca ttgaacattg aaaacattat tgctgaaatt
    12901 aaagaagctt caatccttga attgaacgac cttgtaaaag ctatcgaaga agaatttggt
    12961 gtaactgcag ctgctcctgt agctgttgct gcagctgatg cagctgatgc tggtgctgct
    13021 aaagattcat tcgacgttga attgacatct gcaggcgaca aaaaagttgg cgttatcaaa
    13081 gttgtacgtg aaatcactgg tcttggtctt aaagaggcta aagaacttgt tgacggtgca
    13141 ccagcacttg ttaaagaagg cgttgcaact gcagaagctg aagaaatcaa agctaaattg
    13201 gaagaagctg gagcttcagt tactcttaaa taatagagca actacattag tagcttaaaa
    13261 acatgattaa accgctattc ttaggagtag cggtttttct ttttgtttaa
//
LOCUS       DAVLYR010000041        13236 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLYR010000041 DAVLYR010000000
VERSION     DAVLYR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.041"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..235
                     /locus_tag="VYO63_000933"
     CDS             <1..235
                     /locus_tag="VYO63_000933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671882.1"
                     /translation="FEVVDETDEVSSKHGFEVVDETDEVSSKHGFEVVDETDEVSSKH
                     GFEIVDETDEVSNHTYGKATLTWFEEIFEEYKN"
     gene            complement(316..2139)
                     /gene="lepA"
                     /locus_tag="VYO63_000934"
     CDS             complement(316..2139)
                     /gene="lepA"
                     /locus_tag="VYO63_000934"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1671883.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(2218..2946)
                     /locus_tag="VYO63_000935"
     CDS             complement(2218..2946)
                     /locus_tag="VYO63_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1671884.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            complement(2948..4615)
                     /gene="recN"
                     /locus_tag="VYO63_000936"
     CDS             complement(2948..4615)
                     /gene="recN"
                     /locus_tag="VYO63_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1671885.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGVPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLHLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            complement(4622..5053)
                     /locus_tag="VYO63_000937"
     CDS             complement(4622..5053)
                     /locus_tag="VYO63_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1671886.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            complement(5046..5861)
                     /locus_tag="VYO63_000938"
     CDS             complement(5046..5861)
                     /locus_tag="VYO63_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1671887.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDVKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            complement(5854..6729)
                     /locus_tag="VYO63_000939"
     CDS             complement(5854..6729)
                     /locus_tag="VYO63_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1671888.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            complement(6726..6938)
                     /locus_tag="VYO63_000940"
     CDS             complement(6726..6938)
                     /locus_tag="VYO63_000940"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1671889.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            complement(6916..8256)
                     /locus_tag="VYO63_000941"
     CDS             complement(6916..8256)
                     /locus_tag="VYO63_000941"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1671890.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            complement(8384..9022)
                     /gene="udk"
                     /locus_tag="VYO63_000942"
     CDS             complement(8384..9022)
                     /gene="udk"
                     /locus_tag="VYO63_000942"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1671891.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            complement(9130..9432)
                     /locus_tag="VYO63_000943"
     CDS             complement(9130..9432)
                     /locus_tag="VYO63_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1671892.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            complement(9673..10551)
                     /gene="truB"
                     /locus_tag="VYO63_000944"
     CDS             complement(9673..10551)
                     /gene="truB"
                     /locus_tag="VYO63_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1671893.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            complement(10561..11835)
                     /locus_tag="VYO63_000945"
     CDS             complement(10561..11835)
                     /locus_tag="VYO63_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1671894.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            complement(11931..13190)
                     /locus_tag="VYO63_000946"
                     /pseudo
     CDS             complement(11931..13190)
                     /locus_tag="VYO63_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
BASE COUNT         3699 a         3167 c         2439 g         3931 t
ORIGIN      
        1 ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa catggttttg aggttgtaga
       61 tgaaactgac gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt
      121 cagctcaaaa catggttttg agattgtaga tgaaactgac gaagtcagta accatacata
      181 cggtaaggcg acgctgacgt ggtttgaaga gattttcgaa gagtataaaa actaaaaaag
      241 cagaccatct aagcctgctt tactattgat tcttatataa atttcctgtg aacaaggaaa
      301 ggcatttctg ataacttatt cttcatccat actcaagacg ctgaggaagg cttcttgcgg
      361 aacttcaact gatccgatgg atttcatgcg tttcttacca gctttttgtt tttcaaggag
      421 tttacgctta cgagaaacgt caccaccata acatttagca agtacgttct tacgaagggc
      481 cttgatatca gtacgagcga caatcttgtg tccaatagcc gcttggattg gaacctcaaa
      541 ttgttggcga gggatgattt tcttgagctt atcaacgatg agtttcccac gttcataagc
      601 aaagtcctta tgaacgataa agctgagggc atccaccttg tctccgttga gaaggatatc
      661 cattttcact agcttagatg ggcgatattc tgacaattcg tagtcaaaac ttgcataacc
      721 acgtgtcgaa gacttaagtt tatcaaagaa gtcaaagacg atttcagcaa gaggaatttg
      781 atagataaca ttgacacggt tatcatcaat atagtccata gtcacaaagt ccccacgctt
      841 acgctgagct agctccatta ctgctccgac gaactcctgt ggtaccatga tttgcgcctt
      901 gacataaggc tcttcaatgg tcgcaatctt agttgggtct ggaaactcag atgggttaga
      961 cacatccata gactcaccgt cggtcaaatt aactttgtaa ataacagacg gagctgtcat
     1021 gatgagatca atattgaact cacgctctaa acgttcctgg ataacatcca tatggagaag
     1081 tccaagaaat ccacaacgga aaccaaatcc aagtgcctga gatgtttctg gttcaaactg
     1141 aagactagca tcattcagtt gcaatttttc aagcgcttca cgcaggtcat tgtacttgtt
     1201 tgattcgatt gggtagagac ccgcaaagac cataggattc atctgcttat aaccatgtaa
     1261 tggttctgcc gcaggattgg ttgccaaggt aacggtatca cccacacgag tatcctgaac
     1321 cgtcttgata gacgccgcaa tgtaaccaac atcaccagtc gcaaggaaat cacgaccaac
     1381 cgcttttggt gtaaaaatac cgacttcggc cacatcaaag gtcttactat tgctcatgag
     1441 ctgaatctta tcaccaggtt tgaccactcc gtccatgaca cgcacttgga ggataacccc
     1501 acggtaagca tcgtaaacag agtcgaaaat caaggcctta agtggcgccg tcacatcacc
     1561 cgttggtgct ggtacttttt ctacaatttg ctcgaggatt tcttcaatcc caataccagc
     1621 cttggcagaa gccaaaactg cttcactggc atccaaacca atcacatctt caatctctgt
     1681 acgcacgctc tccggatctg cagccggcag gtcaatttta ttaatgatag gcatgatttc
     1741 caaatcatta tccaaagcca gataaacgtt ggcaagagtt tgagcctcaa ttccttgagc
     1801 cgcatcgacc accaaaatag caccctcaca ggcagctagc gaacgtgaaa cttcataggt
     1861 aaagtcaacg tgccctggtg tgtcaatcaa gtggaaaata taagtttccc catcttttgc
     1921 agtgtaattc aactcgatgg cattcaactt aatagtaatt ccacgttccc gctctagatc
     1981 catgctatcc aaaagctggg cctgcatttc acgacttgaa accgtctctg ttttttccaa
     2041 aatgcggtct gctagagttg atttcccgtg gtcaatatgg gcgataatag agaagttacg
     2101 gatcttcccc tgtcgttttt tcaattcttc taagttcatg attctcttcc tttcagggta
     2161 tctatttatt ataaattgtt tttgatattt tgacaagacc ataccctgct aggagtacta
     2221 atcttcagcg acaaagccgt cattttcgat aaagtggtgt tctgtcattc cttggtctgt
     2281 aaagacaatc ccgtgaagga caccaccata aacagctcct ccatccattc caatcttgcc
     2341 atcttctgta gtccaaagct cagatgtacc gcgttcttgc tgtaacaaac catagaccgg
     2401 tgtatgaccg aagacaatgg tttttccagt atgattttca gctccgtgga atggttttct
     2461 aagccatact tttttataat ctgttgtttc atgccagtcg tccaaagtca aatcaatacc
     2521 tgcgtgaaca aatatatact tgtctgtctc tactacaaat ggcatttgac gaatgaattc
     2581 gaccaagtct gacgcttcag cggcaacccg cttggcatct tctactccat caactggtgc
     2641 atccaaggga cgacctagga tagagttaat ggttgtatct ccaccattgc gacgataatg
     2701 gtcataactt tcttctgggt catctagcca agtcaaaaac atatactcgt ggtttccaga
     2761 caaacagata gccccttgat tgtccaccaa gtccttgacc atttcaagaa cacggtgact
     2821 atcctcacct ctgtcaatca aatcacctag aaagagcaac tgggtctgac catcccaggt
     2881 tttaagaagg tcttccagca tcccagcttt tccgtgaaca tctccaatta cataataatc
     2941 tgtcatctta tttctccctg tttctcaaca attctctggc ttgcgtcagg gctgcttctg
     3001 tcacatcatc acctgccaac atcttggcaa cttcctccac tcgctcttcg actgtcaaga
     3061 gacgaacagt cgaaaccgtt gaatggtcat tactaatctt ctcaataaag aattgataat
     3121 ctgcaatcgc aattacttgt ggcaaatggg agatagccaa aacctgacca tgctgaccaa
     3181 ttttatgaat tttctgagca atagcttgag caacacgacc tgaaactccc gtatccacct
     3241 catcaaagac aatgctagtc ttgccttctt tacgtgaaaa ggcagactta atggctaaca
     3301 tgagacgaga taattcccct ccagaagcaa ccttaaccaa gggtttaaag tcttctccag
     3361 ggttggttga aatataaaac tcaaccattt catttccccc acgactgaat tttcccttac
     3421 taaaatgaac ctgaaactgg gctttttcca tataaagatc ttgcagttct tgtttaatct
     3481 cagcttcgag ttgctgagcc aaattatgac gagcagaagc aagttgacct gccaaattga
     3541 caagattgac ttccaacttc ttaagctctg cttccatgtc ctcagacgaa agattattgc
     3601 ctgtcaagag attgtattct tccgtaatct tggcaaaata aagcaaaaca tcatcaacag
     3661 tcccaccata cttacgagta atagtatgaa ggaggtgcaa acgattctca acctgcatga
     3721 ggcgattgcc atcaaaatca aggtcctcaa tgatagcttc caaacgtttg ctaatgtctt
     3781 ctaaaacata gtaggtctca gacagagagc ttgaaatttc acggtattca gggtcatact
     3841 cttcgacact ttccatgtca ttcatggctg aacgaacatt ggccagactt gaaaaatctt
     3901 cattgtccaa catactgtag gcattggtca gtgtatccgc aatatttttg tggttgagga
     3961 gtttatctcg ctcttgattg agagccaagt cttctccagc ctgcaagttt gctgcctcaa
     4021 tctctgccat ttgaaattcc aacatttcga tacgtgcctt gtgttcctgt tggtttttct
     4081 tgacttccag aacctgcttg cgcattttcc gataggcatc aaaactcgtt tgataggttt
     4141 ctttcaagtc ccaaaaagcg gcatcaccaa attcatccaa catctggata tgcagttggg
     4201 gacgcattaa ctcctcatgg tcatgctgac catgaatatc tacaagatgt tgcccaatag
     4261 ctcgcaaaac agacagatta accatctgac catttacacg gctgatacta cgaccatttt
     4321 gcaagatttc ccgacggatg ataatttcat cacctaattc taaaccttgc tcatcaaaaa
     4381 tttcctgtaa aagacgacta ttctcaactg agaaaagccc ctcaatctct gcctttggta
     4441 caccatgacg aataacatct gtcgtcgcac gagctcccaa catcatattc atggcatcaa
     4501 tgataatcga cttccctgca cccgtttcac cagtcaggac agtcatcccc ttttcaaaat
     4561 tgagggaaat agcctcaata atggcaaagt tttttatcga aatttcaagt aacatataga
     4621 cctaccaatt ttttacttgt tcaaagattt cctctgctag acttccactt ctggcaatga
     4681 ctaaaatcga gctatcatca gtcaaacagc taaaaatctt gtctgcaaaa gtctcgatta
     4741 actgagcttt tacaaaagcc gtatttcctg gaataacttg gagattgatc atcttatcca
     4801 tcaattcagc cgattcgata ttgtcttcag ccagttgcag actttttacg attgattttg
     4861 gtaattcgta gacataggtg ttgtctctca aaggaatttt gacaatacct aactctttga
     4921 tatctcggga taccgtcgcc tgagtggcag tgatacctgc ttctttcaaa tgttctacaa
     4981 tttcttcttg cgtgccgatt tgataatctg tcaccaatct tctaattttt tcaagtcttt
     5041 cttttttatt catttttaaa ttgactatgc gccctctcta ctgcttcttt aatctcagca
     5101 agaatctgat tgcttgctga cttttctttt ttcaaatacg ctaaaaattc aatatttcca
     5161 tgtccacctt ggatgggaga aaagtccaag ccaaggactg aaaaacctgc ctctactgcc
     5221 atagctgtta cagattcaag gacattctga tgaatcttaa catctcgaat aattccattt
     5281 ttcccaatct gctcacgtcc tgcctcaaac tgaggtttga caagtgctac cacctgacct
     5341 tgatcagcca agacacggtg caaggctggc aaaatcagac taagggaaat gaaactcaca
     5401 tcaatactgg caaagctcgg ctcctgctcg aaatcagtct tttcagcata gcggaaattg
     5461 aactgctcca tgctgacaac tcgtgggtct tggcgtaatt tccaagccaa ctgattggtc
     5521 ccaacatcga ctgcaaagac caacttggca ctattctgta gcatgacatc ggtaaaacct
     5581 ccagtagagg ccccgatatc aatcgtagtc gcgccatcca ccgacaaatc aaagacctgc
     5641 aaggcctttt ccagtttcaa accaccacgg ctgacatact tgagtttctc ccccttgagt
     5701 tttaattcgg tgtcatctgg aattttctct cctggcttgt caaaccgttc tccattaagg
     5761 actgctacga ctaggccagc catcacacct cgcttggcct gctctctcgt ttcaaacaac
     5821 ccctgtttat aagctagtac atccactctt tccttagcca ttgattctca aactttctac
     5881 tacacttaca atcgattctg tttcaaaggg aagctgctgg gcaatttctt ctaatttttc
     5941 attagcttga tccagggttt ggttacaaaa ggcaatggac tcttccaagc ccaacaaggc
     6001 aggataggtt gatttttctg cctgcagatc cttttgaggt gtcttgccga tttcctcaaa
     6061 actagctgtc acatccagta catcatctct gacttgaaaa gcaagtccaa tcaattcacc
     6121 cacagttttc agcttcacct gcatttcagg tgacaattca gctataatag ctgccgcttg
     6181 gaagggatag gctagtaact tcccagtctt attggcatga atagtctgaa gttcttccag
     6241 agacaagtgc tggtgttcgc cctccatatc caaaacttgc cctgccacca tacccagact
     6301 acctgaagca agggataagt tggcaatcaa gtccaccttg atctgacttg gcaaatctgc
     6361 ctgcgcaatc aaggcatatg ggtctaagaa taaagcatct ccagccaaaa tggccatagc
     6421 ttcaccgaat ttcttgtgat tggttaaccg ccctcttcga taatcgtcat catccatagc
     6481 aggaaggtca tcgtgaatca agctccctgt atgaatcatc tctaaggcag tagctacctg
     6541 cgcgtgagca ggtttgatgg taacctgcaa ggcttccaga acttctaaca agagaaaagg
     6601 ccgaatacgc ttgccaccag catgaataga atagagaaca gactcccgta aactagaggc
     6661 aaactgctgg tctccataaa aatcttccaa agccgactcg acaagagcta atttttcttg
     6721 ctttttcatt caaaatcact ttctgttccg tcttcttgca tgaccttgac caaggtcttt
     6781 tcagccttgt ccagcgtagc ttggagctct tttgacaaga ccatgccctt ttgaaaggca
     6841 gtaatcgcat cttccagagc aatttcacca ttttccaaac tttggacaat ggtttccagt
     6901 tctgctagat tttcctcaaa tttcttttgt tttgacatct ttaacctcta attctacttg
     6961 accatctcgc atcaaaagcg ttacttggtc ttttttcttc aaactctcaa ccgaatctac
     7021 aacggactct tcttttttga caatagcata accacgcgcc acgattcggc tagtatccaa
     7081 catgagcaaa gcttccgaaa gtcgcttggc ctcagcaacc ttggcgtcat aaactaacgc
     7141 catttggcta cctaagagct tgtccaactg tcctaaacgg tcttgatagc gttggatttt
     7201 ggtaacaggt gataattgta ctaattgatg agttcttgct tgaactaatt gtttgttatc
     7261 agaaatccga gttcgcaaac tttgtttcaa acgcagttgc agttggtcca agcgttgcaa
     7321 ataaccgtca tacaagcgct caggttgtct aaagataaca gactgactgc attttttcaa
     7381 agcctcttgt ttcttagata gaacatttcg gactgccgtt accatccgtt tttcctgatt
     7441 ttgcaaatga gctaatacat ccaacttggt cacaggtgtt gccagttcag ccgccgctgt
     7501 tggcgttgca gcgcgtcgat ctgccacaaa atctgccaag gtcacatccg tctcatgccc
     7561 cacactagag ataactggca aacgagattc aaaaatagct cgtaccacaa tttcttcgtt
     7621 aaaggcccag agatcctcaa tagaaccacc tccacgacca ataatgagca aatccaaatc
     7681 gtcccgttga ttagcacgcg caatatttct agcaatttcc tccgcagccc cttcaccttg
     7741 aaccttggtc ggataaagaa ggatgtcaac acctgggaat cgcctgctga cggtcgtgat
     7801 aatatctcga ataacggctc cactacggct ggttactaca ccaattctct tagaaaattg
     7861 gggcagagct tgcttgaagc gttcttgaaa caggccttct tctgtcaatt ttttcttaag
     7921 ttgttcaaac tgaatcgcaa gcgccccaac cccatcaggc tcagcttttt caatgatgat
     7981 ggagtagcta ccacttggtt catagacctg tacacgccca atcacattga tcttcattcc
     8041 ttcttccagg tcaaacccta atttctgata aatcccagac cagatggtcg cttgaataac
     8101 tgcatggtca tcctttaggg agaaatattg gtgagtaggt cgtttacgaa agttggaaac
     8161 ttgaccagtt aaatagaccc gttccaagta tgggtcttta tcgaatttca ttttcagata
     8221 cttggtcaaa gttgttaccg ataaatactt ttccatctcc acctactatt catttacttg
     8281 ctctttcatg ggtattatta taccaaaaat atgcctaaaa atctccattt atgtaccatt
     8341 atgagggaaa aatagaaaaa ggaggcaagg cctccacatg tgattatttg ctgtttcgag
     8401 cttcttccaa aatctttgca atcttggtcg tcaacaggtc gatagccacg gtattgctaa
     8461 ccccttcagg aatgacgata tcagcataac gcttagttga ctcgataaac tggtggtaca
     8521 ttggtttgac cacacctaag tactggttaa taacgctatc aaggctacgg ccacgctcct
     8581 ccatatcacg cttgatacga cgaataatgc gcacatcgtc atccgtatcc acaaaaatct
     8641 tgatatccat caaatcgcgc agacgcttgt cctccaagac caaaataccc tcaacgataa
     8701 agacatcttg aggttcctga cgataggtct tgctactccg tgtatgctct gtatagtcgt
     8761 aggtcgggat gtccaccgga cgccctgcca acaattcctt aatctgctcg atcatcaagt
     8821 ctgtatcaaa ggcaaaagga tggtcatagt tggttttgac acgctcttca aaggtcaaat
     8881 gagactgatc cttgtagtat gaatcatgct caatcatgga aatcttttca tcaggaaaat
     8941 gcgataaaat ggctcttgaa acactggttt taccaccacc agaaccacct gtcactccaa
     9001 taatgattgg tctattttgc atgctatcct ccgttatttt atccgtcgtc tattctatca
     9061 cattttttac aaaatttata gtctcatcag caaaaaacac cagagtcttc cctctgatgt
     9121 tcgacagttt taacgaaagc cctgtagggt ttgagcaagc atctgcaaga aagccagtct
     9181 ccctctttcc tggctaggag ctttggcaat ccataaacca aaatcaatga cagacttagg
     9241 tgcctggtaa tgatgcgtca tgaggtaaag tgacaaccct tgactcaaat tggccatgac
     9301 cgattggtat ttttcatttc tgtctaaatt ttgtatagca agacgaaaca ggtgactcaa
     9361 ctcactattt tcatttattt ctttttgttg aagaagttgc tccacccttt ctcgaaactc
     9421 atttcgcatc atggaattat cacaccaaat cctgggccta agttcccatc agaacctgta
     9481 atggactcta aatatttatc tgaaagaatt ttaaattgct tcatttcaca cctctaaatt
     9541 tctatataga ttatttgagt acctacaact cgcaacaatt ggaatattat aattcccaaa
     9601 accattctac tattagtatg gggttttatc aagcaattgt tatctatcac ccgattttta
     9661 ttcctaaacg atctagctaa aaaccttcct tggcttatag agattgcccc gtttttctag
     9721 aatggctaac aatttatcat cttcaaaggc agccagttct ttgtccgttt ggtctagctc
     9781 aataaaacga ccaaagcgaa cttctgtagc ctcttctgga gttaggaaaa ctttgacaag
     9841 gtcacctgtc ccaatctcta aaggatggag aaaatctaat tgcccagcct ctactttttc
     9901 agcaatttcc tccaaggcaa gagcgtcttc taattgtaag ccagcagcac tagtacgagt
     9961 caaatgggac atatgagccg cataaccaag cttttcaccc aaatcaactg acaaagtacg
    10021 gatgtacgtc cctttactgc attttacacg aaaagtgaat cgggcaagtt ggccatcata
    10081 agaaatcgga cttgttcgct caaattgata aatggtcacc tgacgttctg gacgctccac
    10141 ttcctgacca gcacgcgcat actcatagag cttgcgacca ttaaccttaa ctgccgaata
    10201 cataggggga atctgagtaa taggcccagt caagctagca atcgcttcat caacaagctt
    10261 ttcatccaag agagacaaaa caggggtttc tgcgaccact tccccactag catcctcagt
    10321 cttcgtggaa tagcccagag tgatttcccc ctcatagatc ttaccctcgt cctgcataaa
    10381 ctcgaccatg cgtgtcgcct tgccaaccgc aatcggcaaa acacccacca catccggatc
    10441 caaggttcca ccatgaccaa ttttcttggt tcccaaaatc ttacgcagtt taaaaaccgc
    10501 atcatgcgag gtcattcctg cttccttttt taagttaata ataccgttca tttttgcttt
    10561 ctactccccc ttcagtgctt cgaacttcgc tttcattgtt ggatttttcc gggtcaattt
    10621 tttaacagag acatcttcca atttgttgtt agctaaacgg agttggtttt cagatgtggt
    10681 caggaatttc ttaacctctt ccatgcgttt gatggccttg tcgatttcat caatagcttt
    10741 tccaaagtta gtcgaagctg aattatagtt cttagcaaaa gctagcttaa aggcatccaa
    10801 atcttcctca aaatgcgtaa tgtcaatatt ttgctcgcga accaaggcca actcctgctt
    10861 gtattttagg gaatttagcg ccgcattacg taagagacca atcaattgga taaagaattg
    10921 aggacgaaca acatacattt tttcatactc gtgactgacg tcaacaatcc ctgtgttaaa
    10981 gtagtcatta tcagcctcaa gcatggtcac caaaacggca tactcacagt tcttctcccg
    11041 acggtccttg tccaattcct tgtaaaaatc tgcattcttg tgcttcttct ctgttccgtc
    11101 cgcttcgttt ttcatctcaa acatgataga aatgatttca actccatttt catcacactc
    11161 acggaagata aagtcccctt tagacccacg cgaagagacc ttgttatcct tctcaaagta
    11221 agcatttgga aaggcgaaac tacgaacctt gttaaactca ctctctgcat actgttctag
    11281 gctttcccca atcgcttttg tagattgttg agccttaaaa ttcttataaa actcgacttg
    11341 ttcactagct gccttgagct gggcttcgta gttttgctta acagaagcca aagataattc
    11401 attttccttt tcctgcaaaa gtagttggtt tttaacctga tcccgttctt tttctaggtc
    11461 agaaagggtc ttttgtagtt gattttcatg ctccaaacgc aaggtagcca actgattttc
    11521 taagagctgt acttccttgt ccttagccaa gagagcctca tggcttgtct gttcaacctc
    11581 tttcttggcc aattcttttt ctgtatcaaa gttttggatc tgactctgta attgcgcaat
    11641 ttcttgatcc ttctgagcca gtttagtctg ttgctcattc atagcctttt gctcagccaa
    11701 ggccagttcc tgcctcatcc tatcgtgtag ttccttatca aactctgccg ttctcacttg
    11761 ggacaaaagt tcagcatact ggctttcatt tactgtaaag acttccccac agttgggaca
    11821 tttgatttcg ttcattacat tcctctttct agacttctgt aaccattata tcatgcctga
    11881 gggaaaatca aaaacagtga gtcaaaaccc actgtttatc ttcttttact ttaaattttt
    11941 tccaaggcca agcgtaagtc ttcaatcaaa tcttctacat tttcaagacc gattgacaga
    12001 cgaatttggt ttggtgtgac acctgctgct tctaggtctt tttctgacaa ttgaccgtga
    12061 gtggttgttg ctggatggac aacaagcgat ttagcatctg ccgcgtttgc aaggtcagaa
    12121 aagatttcta aattatcaat gaccttgcgt gcttcttcct cgtcaccttt gacgtggaag
    12181 gtaaagattg aaccgacacc ttttggcaag tatttctcag ccaaggcatg ataaggacta
    12241 tctgcaagtt ttggataatt taccttttct accttaggat ggttgacaag aaaatcaaca
    12301 attgtctcag cattttgtac atggcgttcc acacgaagtg aaagagtttc aagtctttgt
    12361 agcaagagga aagcattgaa tggtgacaag gctgcacctg tatcacgaag caattgaact
    12421 cgaacagcta taataaaggt tgctgcaccc acatcacgaa tatagctcaa attgtggcag
    12481 cttgaaccct cgtcaacaaa ttgagggaat ttccctgaag ccgtccagtc aaaacgacca
    12541 ctatcgacaa ttattcctcc aatagttgta ccatgcccac cgataaactt agtcgcagag
    12601 tgaatggcaa tgtcaacgcc atgagagaag acgttaatca aataaggtgt tgcaaaagta
    12661 ttgtctgaca caagtgggat ttgatgttta tgagcaatct ctgccagttt ttccaggtct
    12721 ggaatattaa tcaaggagtt acccaaggtt tcaatcaaga caagcttggt attgtctttg
    12781 atagctgctt ctacttcctc caaattatca atatcgacaa aggttgttgt gataccataa
    12841 cgaggaaggg gttctttcaa aagattgaag gttccaccgt aaatagtcga agcagccact
    12901 acatggtcac cagcatgggc aagcgccaaa atcgtataag tcactgcagt catacctgat
    12961 gctgttgcta gcgcttcaac accaccttca agggcagctg ttgtaggatt ggtgatacga
    13021 gtataaatgt tccctggttt cctcaaggca aacagatcag caccttccta cgtgtcatca
    13081 aaaacaaaag atgttgtttg ataaatcggc actgcacgag actcagtagc tggagtcaca
    13141 acttgaccag catgtagttg tagagtttca aatttaaaat cacgagtcat aaggtgacct
    13201 ccatattatt ttattaagga tattgtagca ccttga
//
LOCUS       DAVLYR010000042        13087 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLYR010000042 DAVLYR010000000
VERSION     DAVLYR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.042"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            112..294
                     /locus_tag="VYO63_000947"
     CDS             112..294
                     /locus_tag="VYO63_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1671895.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            239..1088
                     /locus_tag="VYO63_000948"
                     /pseudo
     CDS             239..1088
                     /locus_tag="VYO63_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1140..1502)
                     /locus_tag="VYO63_000949"
     CDS             complement(1140..1502)
                     /locus_tag="VYO63_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671896.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNIEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1793..3673)
                     /locus_tag="VYO63_000950"
     CDS             complement(1793..3673)
                     /locus_tag="VYO63_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1671897.1"
                     /translation="MVRFIGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3667..4536)
                     /locus_tag="VYO63_000951"
     CDS             complement(3667..4536)
                     /locus_tag="VYO63_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1671898.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4620..7265)
                     /locus_tag="VYO63_000952"
     CDS             complement(4620..7265)
                     /locus_tag="VYO63_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1671899.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7356..8636)
                     /locus_tag="VYO63_000953"
     CDS             complement(7356..8636)
                     /locus_tag="VYO63_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1671900.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKNFLLDQLVACDGGTGLMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8807..10891
                     /locus_tag="VYO63_000954"
     CDS             8807..10891
                     /locus_tag="VYO63_000954"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1671901.1"
                     /translation="MKPLLETIDTRFGTASKHAFSWGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(10931..12610)
                     /locus_tag="VYO63_000955"
     CDS             complement(10931..12610)
                     /locus_tag="VYO63_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1671902.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGSNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3653 a         3113 c         2660 g         3661 t
ORIGIN      
        1 tgggttaaaa aagaaattaa ctgagtaaaa actctcgaaa ttcaattcgc tatattatca
       61 ctcaacttga acaaatacca gaaccacaaa aaacagatgt aaaggaaaaa tatggactta
      121 aaatttgaag gggttgattt agaatacaaa aaagctaaaa acaatcttcc agagtctttc
      181 tgggaaacct actcagcatt tgcaaataca aatggcggga aaataattct cgggatagat
      241 gaaaaaaaca ttgacaccta ccaaagagtc aatagactcc ctgcgaaatt atgaagcaag
      301 taaacaatta actgatacac gatttaagcg tcttgttggt gttcagcgca cgacttttaa
      361 agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa
      421 acctaaatta agcctagaag accttcttat ggccactctt caatatgtgt gagaataccg
      481 cacttatgaa gaaattgcgg ctgattttgg tatccacgaa agcaacttaa tccgtcggag
      541 ccaatgggtt gaagtaactc ttgttcaaag tggtgttacg atttcaagaa ctcctctcag
      601 ctctgaggac acggtaatga ttgatgcgac ggaagtacaa atcaatcgcc ctaaaaaaac
      661 aattagcgaa tcattctggt aaaaagaaat ttcacgctat gaaggctcaa gcgattgtca
      721 caagtcaagg gagaattgtt tctttggata tcactgtgaa ctattgtcat gatatgaagt
      781 tgttcaaaat gagttgcaga aatatcagac aagttggtaa aatcttggct gacagtggtt
      841 atcaagggct catgaagata tgtcctcaag cacaaacttc acgtaaatcc agcaaactca
      901 agccactaac agctgaagat aaagcctata accatgcgct atccaaggag agaagcaagg
      961 ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc
     1021 atcgtaaacg cttcggatta cgaatgaatt tgattgctgg tattatcaat catgaactag
     1081 gattctagtt ttgcaggaag tctattgaaa tactaagcct acacagactg atttcaattt
     1141 taccattctt catagtcaaa accatcataa tctggatatt gttctggcga cgttccaacc
     1201 gaacgtacga gataagcttc ttcgtcctct gtctcgattt ctctcagcac ttggcattca
     1261 aaacgccatt catcaccgaa gtcgaaaata aacttaaatt tttgtttgac acttaggcta
     1321 tccagataga cattttctgt gtaacgttct tcaacatcgt cactaacaaa gctaagaaag
     1381 taagaatctg catgactcca ctcaatatta tccatgaaaa atgcgtgagc atgatcattt
     1441 gcaaaatcaa aactccataa aatcacatct gctagttccg ctaacgtttt tgtttttgga
     1501 atctatatat gtcggtagca acctttttta aatttgacgg aaatgatcca agcctctaca
     1561 gatgctggct tttcagcttc tatttcttgt tttactaatt ttaactgtag catttgaaac
     1621 cagataattc ttctgtttca cgcgaaacac cctgcgccaa gcaaggtgtt cttgttttta
     1681 tactcaatga aaatcaaaga gcaaactagg aagctagctg caggctgctc aaaacactgt
     1741 tttgaggttg tagatggaag ctgacgtggt ttgaagagat tttcgaagag tattaagtcg
     1801 tataaatcgt cgaagctgtc gcaatggtat gccactggtt agctgtagtt gctgcgaaat
     1861 ccttgtctgc gtagaagtca gtaaatggta ggatttcaac ttccagctcg tcgatgcggt
     1921 caagctggcc agccagataa acctcgatac ggctttctgc tcgtttgatg cgttgcaaga
     1981 gtccgcccat acggatgtca actgtatcca aaccaaagac cttgttttct ttcagccatt
     2041 ggtgactaaa gagggcatgg aagtcttcaa tttggcttct aagttctggt aattcttgtc
     2101 tggcgatttg ttgtaaactt tctttatcat ccgcttggta ggcctgacga atgcgtcgtc
     2161 ccacatctac tttgctactt aaaatagcat tcaactgggc ctgagtttca aagagataag
     2221 catagtttcc agctttttct ttaatgttag caagcgtctc agcagcctga gcgaagtgcg
     2281 gtttgtcctg ttcaggtgtc atgtgtcggt caagtatcgg acagagaaca tcctgataaa
     2341 agacatatcg gttgggattg atgccactga gattgcctgg taggtctggt aagaggttgg
     2401 caaggtcaat ctgcataaaa tcctcaaccg ttagaccagt attggtcttg aagtgcgcag
     2461 acaaactatc taggtcatgg cgatagctga gttctgccca gatttgcaag cttggtagga
     2521 tagagaactg ggcagtttca cccccattgt ctccccaacc cgttacgatg acttctttaa
     2581 tctgattggc acggcaggct ttattagcct cgatagccac tagacggcta aaatggttgt
     2641 gaggtgtaaa gccaatccac ttccaagctc cccctgcaaa tgcaaggtca tggctaatct
     2701 tgtgatgatt gcggaaatta cggttgtatt tttcctcgct atcctgataa taatcccagt
     2761 aaaccagagt cacacggtct ttgagacggt ctaggtagac acgagtttcc tctggaattt
     2821 ccacatcacg gtcgtactgg ccatccgctg acatgagttt gaagaacata tcactccaca
     2881 tctggcagtg gaaaccatat ttgtcagcaa tatccagcac gcgctccaag tgttggcaca
     2941 tgaggagact acgatccaca acaccgttca aaatcaggta gcgtcccaaa ccaaccaagt
     3001 gggcttcgtc catcccgata ttgaccttgc gagtcttcag tttagacaac gtggcaaaca
     3061 tgccatcaat caggtcataa accttttctt cgccaatgag aagaatgtcc tctacatcac
     3121 ggagctcctg cacttccttg acaccccatt tgacaaaggc cgacaagtgg gccaaggtct
     3181 ggatgcatgg tacaaaggtc atgtcaaact gttgggcata ggcttcgatt tcctgcaact
     3241 cctctgctga ataagctcca cggaaatagc caaagtaagg ctgtccttca atctggtaag
     3301 tgtcttccat gtaaagctca aaggttgagt agcccatgag agccaagacc tcaatcatct
     3361 gcttggcaga agccacattc agcaccgcat ttcgagaaca gtcaaccatg taagccaaat
     3421 cttcgtaagc tgcttgttcc tcaatctcta ctttatcagc ttctgctaga actgttacca
     3481 acaaggacaa ggcacgataa agttggtgag gtttgcgata ggtcaattga tagtgacctt
     3541 cctcaccctg gatagagata gatgcttggt cagactgagt gacagccact tccacatctg
     3601 gtagagaaat gtgacctttt aaaacctcaa tagcttgcgt ttgtttggga ctaagtccta
     3661 taaatcttac cactgctttt cctcctgtag ccagttgaca agagcaccgt agagattggc
     3721 atctgcgtga taggtgcagg cctggataac tggtgcgacc gtgtattctt cgtaggcatc
     3781 gacaaagtct tcaacagcct tcttaacacc ttggataaaa tctggatttt gactgataga
     3841 gccacccaga ctgatgacat ctggatcgat gagatactgg atattgagca agccttgcgt
     3901 caaattgcgg ttcatgcgct caatggcttc ttgacaaaga gcattaccag ctgcggcctc
     3961 ttggtaaatc ttgcgaccgt cccaatcagt atgaccagat ttttcaatca cgtatcgtac
     4021 catattccca gttgatgcta gttgcgacca gttattaagt ttttcagcag gggcaagggt
     4081 tgtcatgtag ccaaattctc cacccagacc gtggcgacct cgatgaagtc taccattgat
     4141 aatcatggct ccgccaatcc ctgtcccaat cacgacacag gctgcatttt caagctctgg
     4201 atgagctagt agttcactga gtccaacgca gttggcatca ttttctaaat ggacaggtag
     4261 ctgataagag ctaagcgcct cataccaaga aaagccatgg atgtagggca ccgcactgaa
     4321 gccatcaatc acacctgtct cttgattgac tgcacctgga acactcatag caatcccact
     4381 gtaatcctgt tctgacaagc gttgatctag ccacgctagt aaatcctcca agttttcagg
     4441 cgttgaaata cttgtcttat ccagtatttt cccatcagga gtcagactgg caaacttaat
     4501 cccagtccct ccgatatcaa tcgttgcaat ggtcattgtt tttaccataa aaaggggcga
     4561 atcagcagtt agttcttact ttttcgcccc tcctttcttt tgatgtttac tttttgaaat
     4621 taaatttctt cttttttgat gaattctgta cgaatctctt gtggagccaa tagtcctgaa
     4681 tgaactggat atggtcgctc aagtaagtca agaatggttt gttgatgttc tgatatgcgc
     4741 acattttctt gactcatatt atagtaacgg aggacatagc cttcttcatt ttccgccact
     4801 ttaaaggctg ttggacaaac ttgcggtatg ctgagaacag aatggctcaa gaggctacca
     4861 gtcgcaacca cgcttccttc ctgtctagca agctgaaggc tggtaaacgg tgtctgcaag
     4921 gctttggcac gacgataggc tgagaagcgt tcttgggctt ggtggcattc aatcgcaaat
     4981 tcgacttcaa actcacgcaa gcattgtgct tctggcgttg ggaagtagcc ccagtcacct
     5041 agctcacctg atgcacgcaa aatggtcacg gcaatggtgt tatccccaag gatttcgtat
     5101 tcattcaatc ccttgttgga tacagtcacc cctttttcat cgtcatacag actgacaaaa
     5161 gcttgttggt gttgaggatt ttcaggattt tcccatgaag cagctggttt gtttggtcgt
     5221 gtcaccacct catagatact ttcagaatca ttgcttggac gcgtgttatg agtcttgacc
     5281 aagagacgga tacggtgatc cttggcagtg ttagtaaagc gagtcttgaa gcggatttgt
     5341 ggattgtcaa caaagacaat caactcagtt tccagaggaa tgtttgtcaa ttcttctgac
     5401 cgtccagcct cacgcttcat aaactcgatg ataccttgtt gctcttcttc tagcttttca
     5461 tccgcactga caggcacggt caattcatgt ttgagcaaga ttttagcata gcgatctgtg
     5521 ttttccaaga cctcgtggcc cttaagctct gcaaagattg gctctgttcc ttttggttgg
     5581 aaatagatat actcgtttcc gatgtcaccc cggtcttcaa agcggataaa gtcttcatag
     5641 gcttcgtgag ttgtcttgtc atagactgtg atgttgtcat ccacactcac cgttacgaat
     5701 ggtgtatcaa tcactccgtt ttggtaaata ccctcacggt gttcttgttt tccttccagc
     5761 aattggaagg ttgtccaaga aagcggcgct aggtgaactg gaatggtcac gcgcacttgt
     5821 cgagcaatac gagcttggcg gaacttatct tttggtaaat cgtactcaaa attagctccg
     5881 aggtcttcga ttgtagcctc tacaggacga ccatccaagt cctccacacg gtaacttggc
     5941 aagataagag cagccatctt tttgtagcct tctgttgggt gcaattcctt gaaatcacaa
     6001 gtcgccacat caatcactgt gctgacagta tcgaccttat catgcaagcc tgtgttaatg
     6061 acagtaaaga gatagtcact ttgagcctta tccgtagcaa ttttaccctt ccactcgttg
     6121 agcaagttac ttttaacaaa gtttcctact tggttgacct tggcaaaacg cgtttccatc
     6181 tcgcggtgaa cttcgtccac gctacagcca cagatactat catgtggcgc gttctgcaaa
     6241 agtgttttcc aagcataggt caactggtcc ttgtggttgt gtccaccagt gataatagtc
     6301 aagggttcta ccacttgctc taggaggttg ctattttctt ggaaggcttg tttcaggtaa
     6361 atgcgggatg aagaagtgtt ggcaagtgtg taccagccat ctgtttcctg actggtcaac
     6421 tcgcctgtaa cagttgataa gtgttcagga agcgcacctt ctacagcttg aacatattca
     6481 tcaaaagaac tatgaacaaa gattacatcc gggaagagtt catttgccac acgaatggct
     6541 tcgctcagat ttttctgtac aggctggtgg tcacagccgt tcatcatcaa ccattggttg
     6601 gtcgaagcgt aggcacgcac atctgacaat ttttgtttcc agaaggtcaa ggcctcatct
     6661 ttgtcaactg ggatttcatt cccattactg taccagttgg caaagagaat acctaaaaca
     6721 cgactaccat ccacaccctg ccagtacatt tctgaaaact gggatgtaaa ctgctcatct
     6781 tcaaggactt ggttgtcaaa tccaatcggc ttcacaccac gaccaaaggc cgccacatga
     6841 atgcctgatt tttgaagaat ttgaggtgct tgtcccatat ttccaaaggt atctggaaag
     6901 tagccaatct gggttgattt accccatttg gcagcttctt gttgaccaat caaggtattg
     6961 cggacattgg cttcacttga aatcaaataa tcatcctgca agatgtaaaa gggaccaatt
     7021 ttaagcttgc cctcgtcaat gtagcgttgg accttgtcgc gattttcagg gcgaatttgt
     7081 aagtagtcgt caaggacaat agtttgtcca tccaagtgga aactcttgaa ctcagggtca
     7141 ttttcaaaga gatcaaagag attgtcaaac aattccacca actgcatacg atgactttca
     7201 aaaggcaagt accactcacg gtcccagtga ctatgtgaga taatatgtac aacaacattt
     7261 tccatgaagt aaaacctcat tctaaattta aattttaacg ttttaaatgt aaacttgtga
     7321 ttctgataag aatcggttga gctaaagcga gccccttagc gaatatccaa gtaatccaag
     7381 accaactcac agaacatcat gttagcccag gagaaccatt cacgagagta gagagttggg
     7441 tcatccacgt ggaagctttc atgcataaga cctgtaccgc catcgcaggc aaccagctga
     7501 tccagcaaga agtttttctc tgccttatct cttgttgtca agccttggat agaaagggcg
     7561 attggccaga tatagcgata gaaggtatga gaacttccaa gaccgctagc gtattctcct
     7621 tggtagaaat atggattttc agagctcaga atggtacggc gagtagcttg atacacttca
     7681 tcatcgaccg aacagtagcc cagatagggc gcagctagta gacttggtac gtttggatca
     7741 tccataatgc tggcatttcc taggccatcc acttcaaagg cataaatctt ttcaccctta
     7801 ctgttggtgg tgtaagcgta gttttcgatt ccttcttgga tttcatcctg aagacgcttg
     7861 gcatcagcaa taacactctg gctatcagct aggtttaatg ctgcgaagat ttcttgcaca
     7921 taacccaaga ctactacagc aaacatattt gacggaatca agtaactata ctggcaacag
     7981 tcatcactcg gacgaaaggc tgaccaagtc atacctgtca ctgcaaagtc aggtccaaag
     8041 ccatcattta ccaaggtgtc ttccttacgg tccgtatctc ggacaaaacg ataaggagag
     8101 ttcttgtggt cttgttcaac tgtccataga tggagaattt ccttagtcgc tgcgacaaaa
     8161 gtctcatcaa actgactagt ctcgccagtt tctttccaga ggagataagc caactgcaaa
     8221 ggatagcaaa gcgagtccac ctcatacttg cgttcccaaa tccagccatt aaggtcggtg
     8281 tggtcggtct cgtggtgccc cttccagttt tcctctatgt tgaaggaatt ggcataagga
     8341 tccttgagta ccaaggtcat ctgacgtttg accaaacctg caatggtctg acgcaggagg
     8401 gcatctcttt tagctacatg caggtagggt ctgagctggg ctgtcgaatc ccgaagccac
     8461 atagcaggaa tatccccagt caagacaaaa gttgaaccat cttctaagat ttcaaccgta
     8521 ttgtccaagg tatcggtgta gcaacgctcg aaaacatcca cccactctgg atggtcctta
     8581 gcccgctctg ctacttcatc tagccattct ctaacaattt ctttcgaata aatcatcgtg
     8641 caattccctc tttcaaacaa gtttcatgtt cagtatatag cttttgagac agaaaataca
     8701 tacacaaatg atagtcattt ttctacaaaa tagttatttt tgaaactcct tttctaaagt
     8761 ccctcttttt ctgatataat gtagaaaaac agctaaagga aagactatga aaccactact
     8821 tgaaaccatc gatacccgct ttggtactgc tagcaagcat gccttctctt ggggaaatac
     8881 cctgccatac acaggcgtgc cttttgggat gaattacttt gtgccccaga ccagtgacca
     8941 ggatggatct tggttttttg atccgcatct gcctatcttt caggggattc gattaactca
     9001 ccagcccagt ccttggattg gggactactc ttggctcctt ctgacacctg tcaccagtca
     9061 actgggggga gacagcctct ttcaccgcca gtcttcttat gatatagata aagcttgctt
     9121 ccagcctcac tatttgaagc tcttttctct gcgttatcag attgaaaccc agctcacacc
     9181 gacttgctac ggtgcttcta ttcgtttgaa gcaaaagcaa ggcaaagccc tctccctcta
     9241 tcttcacgca acagatgaac tgacagtaga gcaagtagat aagcgaactc ttgccctgcg
     9301 acaagaaggt aaaactgaaa ccaacaaaaa ttcgctaacg atgttcactg ccctgcaaat
     9361 gaacacggat attcttgcta tcagccaaga agctggggac tggcgaattg acttagcaag
     9421 tagtcaaacc gagatgcaac tagccacttc tttcatctct ccttctcaag cactgcttaa
     9481 tctacctcaa gaagattttg atagctgtaa agcaagtgcc caagcggatt gggaaaatct
     9541 cctccatcgt tttgacatta tagagacagg agaggctgac cgaaccttct ttgaccactg
     9601 cctctacaga ctcttcctct tcccacagac tttttatgag gttgatgagt cagggcaagc
     9661 catccacatg gatctggcta ctggtactgt caagcctggt gtcctcttta gcaacaatgg
     9721 tttctgggat accttccgca ccaccttccc cctctttgcc cttatcatac cggagcacta
     9781 tcaacgcttt ttagaaggtt tcctcaatag ctaccgcgat actggtttcc ttccaaaatg
     9841 gctggctcca gatgaacggg gtatgatgcc aggtacactt ttagacggta ttatcgcaga
     9901 tagcgcctgc aaggacatgg cccctgacct agaaggagaa ctcttccaag ccatgctcga
     9961 aacagccagc aaagccgacc ctctcggaat caatggccgc cacggactag cccaatacca
    10021 agaactaggt tacctctcta ccgaccacca cgaaagtgtt agccatactc tagactatgc
    10081 ctatagtgac ttttgtatcg ccagctgtgc caaaaaacta gagaacatag aaatcgctga
    10141 aacctacaag gctgcttcac aaaattaccg ccagctattt gacgctgaga caggttacat
    10201 gcgagcacga gacaatcaag gaaactttca ccctgacttc tctccttata gttgggggcg
    10261 agactacgct gaatgctctg ccattcaagc tactttaggc gttctccacg acatccctgg
    10321 cttaatccaa ctgatgggtg gaaaagaaac ctttagcaac tatcttttga aagcctgtca
    10381 agatgctccc ctctttgaaa caacaggcta tggttacgaa attcacgaaa tgagcgagat
    10441 ggctactgct ccttttggac aaatagccat ttccaaccag cctagtttcc acattcctta
    10501 tctcttccgc tacagcgatt accctgacta cactgccctt cttatcaaga cgctccgtca
    10561 gaaagctttt cacccaagtt gggaagccta tcctggagat gaagacaatg gtagtctctc
    10621 tgcttggtac atctggtcag ctctcggatt ttatccaacc tgtccaggaa aaccaagcta
    10681 tgacctcgga atccctctct ttgaccatct acgagtctac ctcgctaaag aagataaatg
    10741 gctggatatc catactaaac aaaaccacaa ccattttaac tttgtcaaag aatgccgact
    10801 ggacaaaaca ctcgtatcaa ctattcaaca ccaagacctc ttaaaagctg aacaactaac
    10861 tttcaccctc agctggttac caagtcacta ataaccaaaa gaacctttgc atcgcgcaaa
    10921 ggttcttctt ttatgaaact tggacttgaa gttgatccac aagctgacca tccaccgtta
    10981 ggttcagata gaaatccaag gttttatctc ctgcgaaata cagggttggt agatgcaaat
    11041 cttttttgct ttcccctgct tggaattgaa ggacttgaat ctcatcctga taggcgacac
    11101 catggacacc tgtccccggt tgaatcgaaa tcttagcctc taaagaggag ctactttcac
    11161 tacgttcaat cctaaaatga agagtctctc cctttgccac agctaggctt ttttctgcaa
    11221 atgctagccc ctgaacagct actgtttgtg ataacctcgg agttttatag agtgaaatct
    11281 tggtcaacaa aggcaaagcc tgtgattctg taatgactac acgtatcttc tgtgcttcaa
    11341 caactgctcc tcgtaagaga cgtttgtaac caacagtatg acccgaacca aactcctgcc
    11401 agacaccatc cacctctact tgcacatgaa aagcagcgat tcgttgccct agcttcaaat
    11461 cttctcttaa ctcaattaca tcaaaagttt taggagaacc taagtcgagt tctaactgga
    11521 tgggcaagtc tgcatcgctt gcccaagagc tggtctcaag gccgtctgtc aaatggcgac
    11581 aagcaaagtc tgcggaaaga gctggaccag atacctcagc tcccagagcc aaatcttctt
    11641 tatagagctc attgcgatag gtcgcaaatt cataaagtcg ttcaatatcc tttgcatcaa
    11701 agagcccagc ttgattcggc ggaatattaa gcaagagtgg agttcctcgc cctactgagt
    11761 gaaagtagat ttcgaccaac tcctcgagag acttaggatc ctgatcctca tggtagaacc
    11821 agcctggacg gatggaaaca tctgcctctc cgattgaaaa aatcgtgccc gagggatccc
    11881 cgtgctgaag atagttcagc tctgcttctg ttcctagttt atcaggattc accttttgcc
    11941 acagtggatc acctgcatac cctcgttcat tgccaatcca gcggatactg gtgccttctg
    12001 ttgaaaaaat caagcaagcg ccctgcaggt cacgaatggt ttcaaaccat ttttcaaatt
    12061 cataattaac cttttgcgcg ccctctcctc tggcaccatc catccaaacc tcagcgaact
    12121 taccagcatt cccatagtta ggatttgata agatttcctt caactgagcc agataatagg
    12181 cattgtagtc cgcttctcgg tccacatgat agaggggact atgggcatcc cacggtgaca
    12241 ggtagacccc catatccata tcaaactctg tggcagcttg ggatacttca aggagcaagt
    12301 cgccctttcc tctcctccaa ggactgacct taaccgaata atctgtgtga gctgtcggat
    12361 aaaggacaaa gccatcgtgg tgcttgacca ccaaaatcaa ctttttgaag cccgtttcct
    12421 tgagcacacg aacccactca cgcgcatcca actgactcgg gttaaagcgc tcaggatcct
    12481 cctgtccagt cccccattct tggtcataaa aggtattaga accgaagtgg ataaaagctg
    12541 ctagttcatc tcccagataa gctagctgag tctgacttgg taacggtcca tgcggtttga
    12601 ttttcttcat cgttatatat ctccttcttt agcaacttct cttgttcaca aggttatcca
    12661 caggttgtgg ataataaacc atgatctaac agccagctct ttccaactcc aactggtagg
    12721 ctcaggattt ttctcctcaa tattccctct tcaaacagct ctgtcatatg gttctgctaa
    12781 cacctctcag aatgctactc tccataatta ttctcaggct tctttcaggt cattgtgtga
    12841 agaccattca ctctccagac ttttccacag attgtgaata agtgattatt ctaaactatc
    12901 acctcgtcga ctataacaaa aaataaaaag agggtcaata agataattat ctcttcctaa
    12961 atactcagaa aagattctat aatttgtaca attgaacaag aaatgcttct ccaaaatcta
    13021 tcttcaaaag aatgttgcat ctttttatcc tttcacacaa acatttctct ttatacagat
    13081 ccaagtg
//
LOCUS       DAVLYR010000043        12953 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLYR010000043 DAVLYR010000000
VERSION     DAVLYR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.043"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            113..1459
                     /gene="celB"
                     /locus_tag="VYO63_000956"
     CDS             113..1459
                     /gene="celB"
                     /locus_tag="VYO63_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV1671903.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVIIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDVFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            1839..2015
                     /locus_tag="VYO63_000957"
     CDS             1839..2015
                     /locus_tag="VYO63_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671904.1"
                     /translation="MSLDIDKEKMTIMGIDFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            2416..4629
                     /locus_tag="VYO63_000958"
     CDS             2416..4629
                     /locus_tag="VYO63_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1671905.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSKTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAIPLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNKNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPMYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTI
                     PVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELTLQGDSSIVPSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(4851..5327)
                     /locus_tag="VYO63_000959"
     CDS             complement(4851..5327)
                     /locus_tag="VYO63_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1671906.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            5550..8629
                     /locus_tag="VYO63_000960"
                     /pseudo
     CDS             5550..8629
                     /locus_tag="VYO63_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            8982..9367
                     /gene="tnpA"
                     /locus_tag="VYO63_000961"
                     /pseudo
     CDS             8982..9367
                     /gene="tnpA"
                     /locus_tag="VYO63_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9746..10375)
                     /locus_tag="VYO63_000962"
     CDS             complement(9746..10375)
                     /locus_tag="VYO63_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1671907.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(10385..11386)
                     /locus_tag="VYO63_000963"
     CDS             complement(10385..11386)
                     /locus_tag="VYO63_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1671908.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11417..12058)
                     /locus_tag="VYO63_000964"
     CDS             complement(11417..12058)
                     /locus_tag="VYO63_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1671909.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12077..12892)
                     /locus_tag="VYO63_000965"
     CDS             complement(12077..12892)
                     /locus_tag="VYO63_000965"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1671910.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4039 a         2211 c         2758 g         3945 t
ORIGIN      
        1 gtccaattgg aaaagaactc acttagttta taatcctgat ctagtataat ttgtatttga
       61 aactagtctt tagaaagtaa aaattattat gttttattaa ggaggtctat ttatggatca
      121 acaaaacggg ttgtttggtt ttcttgaaaa ccatgttatg ggaccaatgg gcaaacttgc
      181 tcagtttaaa gtagtgcgtg ctatcacggc tgcaggtatg gctgctgtac catttactat
      241 tgtaggatca atgtttttgg tattcagtat tttgccacaa gctttctcat tttggccaat
      301 tgtggcagat attttctctg cttcatttga taaattcaca tcactttaca tggttgcaaa
      361 ctatgcgact atgggttctc tatctcttta tttcgttcta tcacttgcat atgaattgac
      421 aaaaatttat gcagaggaag aagaactcaa tatgaatcct cttaatggtg ccttgcttgc
      481 cttgatggct tttgtcatga cagtaccgca aatcattttt gatggtggaa tgatgaagac
      541 tgtgacaagt ctaaaagaag gtgcagtaat tgcagatgga tgggcaatgg gaaatggagt
      601 cgcacgtttt gggacaacag ggatttttac cgcaatcatt atggcaattg tgactgttat
      661 tatttatcgt atgtgtgtta aacataattg ggttattaaa atgcctgaag ctgttccaga
      721 aggagtttct cgtggattta ccgctttggt tccgggattt gttgttgcat ttgttgttat
      781 ctttatcaac ggtcttcttg tagcaatggg aacagatgtt tttaaagtca ttgcaattcc
      841 atttggtttt gtatccaatc tgactaattc gtggattggt ttaatgatta tttatttact
      901 gactcaacta ctttggattg taggtatcca cggtgcgaac attgtttttg catttgttag
      961 tccaattgct cttgctaaca tggctgaaaa tgctgctggc gggcacttcg ctgttgcagg
     1021 tgaattttct aatatgtttg taattgcagg tggttctggt gcaactttag gactatgttt
     1081 atatattgct tttgcctcta aatctgaaca gcttaaagca ataggacgag catctgtagt
     1141 tccagcctta tttaatatta atgaaccatt aatttttgga ttacctatta tctataatcc
     1201 agccttggct ataccattta ttttagcacc aatggttact gctactattt attacgtagc
     1261 gaattctcta aactttatta agccaattat cgcacaggtt ccatggccaa ctccagtagg
     1321 gattggagct ttcttaggga cagcagatct tcgagctgta ttagttgctc tagtatgtgc
     1381 atttgcagca ttcctagtct atcttccatt catccgtgta tatgatcaaa aattggtgaa
     1441 agaagagcaa ggtatctaat aagtaaaaaa gtatactcga tgatagaatc catatagttt
     1501 aaataatgag cgttaacatt agcagtgaag aggtcttttt aagatattct tctctgcttt
     1561 ttcatatttt ttcgctatgt agggtatgaa gcaaaataga aagtagttcg aatagttgat
     1621 aaacagggaa ctgctgttat tttaaaaaaa caataaagta aagtatgtta tcgtagaata
     1681 cgatacaata gtaaaaaagc aaaaagaatg cgttttatat ttgggtttga ggcttataaa
     1741 ataggataag catgctgaaa acttagatat gacaaacaaa ataaaatttt taaaagctac
     1801 atagaaacat ggtataatgt atctaaagga ggtagcatat gtccttagat atagataaag
     1861 aaaagatgac aatcatggga atagattttg aaaaccgatc cgtttttaag agtgtttggt
     1921 atgctttaag tacaaatatg attgaaggat ggcgacctac ggttagtgat gttgaaaaat
     1981 tacgagatga agctcttgct ctggggatga cttaatgaag cgcaactgtt atgaatctta
     2041 tgactatgtt gatccggata atttatatac cgatccgggt tcttcagttc taagaaataa
     2101 gcaggaagga aaaaagcacg tgagcttgaa tattgtatga ttgctagtaa gagtttgaaa
     2161 ttatttatca atcctatctt agttaatagt ttaaaagtag tttgtttcac aactgcataa
     2221 gcttagaaaa gttgaaaagt gataactctt taataaactc ttggtttgtt aaaagatatt
     2281 ttgtccacta cattttttca attgactatt atttgtcata gatataacag atagaacatt
     2341 gcattcagaa taatgaaaaa cgcatcaaaa atggtgcgtt ctgtttttat gctaccataa
     2401 aaggagggaa acaaattgaa aatttttaag ggagagtttt atcgcatctc tgtattaaca
     2461 gacaagctag taaggttaga atactctaaa actggcagtt ttgaggatag aactacacaa
     2521 cttatctata atagagattt tggccaagtt tcgttagatt atatcgagac atcaaacgta
     2581 ctagatatta tgacggacta ttttcatctg cactttaata aaggagaatt taacgccgaa
     2641 aatttattta tagaattaaa aggaaatttt gccgtatatg gtagtcgctg gtattttggt
     2701 gaatcgattg aaacgttaaa aggaacagct cggactctgg ataaggcaga tggagcaatc
     2761 ccattagaag atggaattat tagtcgaaat ggtatagcct tattggatga ttctcaagga
     2821 tttatttggg atgaacagtc tggttatatt gagagagaaa atcaaattga cctctatttc
     2881 tttgcctatg ggcatgatta tagaggagca atcagagact tttaccattt gactggttca
     2941 acacccttgt taccaagata tgctttaggg aattggtgga gtagatattg gccttatacg
     3001 tcggatgaat acttggattt aatagacaga tttgaaacag agaaaattcc attatctatc
     3061 ggtgtgttag atatggattg gcatataact gacattccag ctcgttttgg aagtggctgg
     3121 acaggatata gttggaataa aaacttaata cctaatccag aacagttatt gcaacaactt
     3181 catgatagaa agttaaaact ctctttaaat gtccatcctg ctgatggtat acgggcttat
     3241 gaagaagctt atcctcaagt cgccaaacga ttagggttaa atgtagaact agaagaacct
     3301 gccatttttg atttttttaa tccctctttt agggaagcct actttaaaga tgttcattat
     3361 gagctagaaa agcagggagt agatttttgg tggattgact ggcaacaagg gactcaaggc
     3421 atattggatc cactatggct tttaaaccat tatcattatc aggatagttg taaaaatgca
     3481 gaaggtggtt tgattttatc aagatatgca ggtcctggta gccaccgcta ccctgttggt
     3541 ttttcagggg acactattat tagttggaat tccttaagat ttcaacctta ttttacagcg
     3601 acagcatcta atatcggtta tagttggtgg agccatgata tcggtgggca catgctggga
     3661 gattatgacg aagagctgca aactagatgg ctacagtttg gtgtttttag tccgatcact
     3721 cgattacata gttctagaag tccttttaat agtaaagaac cttggttttt ttcagaaaca
     3781 acatctaaga ttatgaagaa ataccttcgt ttgaggcatc agatgattcc atatctatac
     3841 actatgaatg taaagacaca cgaggaaggt gccccattaa tcagcccaat gtattatttc
     3901 tacccagaga atgatgagag ctataatgtt ccaaaccaat acttttttgg aacagaacta
     3961 atggtggctc ccattgtaga aaagatggac ttggcattcc aatctgcaaa agtggatgta
     4021 tggttccctg aaggtgaatg gtatgacttc ttttcagaga aaaaatatac aggtggtgtg
     4081 aagttgagtg tttatagaga catttcgact atacctgtgt ttgcaaaaag tggtgcgatc
     4141 attcccttgg ttggttctga gatagatatg ggtgttgatt tacctgaagt tgtagattgg
     4201 tatgtattcc caggcaaaca acattctttt gaaatgcttg aagatcaaaa tggtcaaaga
     4261 tataaaacaa gattatcaat cgattgggaa atggggatgg tagagttaac attacaagga
     4321 gattctagta tcgttccaag caatagaagg catagaattc attttaaagg aacgaatgtg
     4381 tctataattg aattaccaaa taagaatgat acagctagat ttgaatgtaa agataataaa
     4441 acaatatctt taaatgatga agtttttaga ctactaaaga cagcttctct tccatatgaa
     4501 ttaaaagata gattgttaaa tcaattcatc aatgcgaaaa actctcatga cttaatgaat
     4561 atcttgcatc atcaggataa ggaattgaga gggcgtttgt tggaaatgat atttactagc
     4621 caaaactaat tgatataatt agaaagtcat ttgtttagtg tatatgaaga ttctgtatgg
     4681 tattcgttat cactggtcac attgaaaatg tgaaataagc acctacaaca tagactttac
     4741 aacacttcat gtttcatatc atggggtgtt tttctattta agtcaaagcc aatcaaaaaa
     4801 tctcactatt catcaggtta aaggaaacct aatcatagtg agattgtgaa ttatagtaga
     4861 gtttgtatcg cctcttcaat ttgttttggg tctgtttttg aagaaaagcg ttcaaagact
     4921 tgcccatctc gaccgatgag aaacttagcg aaattccatt cgactcgttt tcctagtggg
     4981 ccggatttct ggtcttttaa ccagacatag agagggtctg cttccttacc gttgacctta
     5041 atcttggcaa aacgtgggaa ggtggtttga aaatgtaggc tacagaaggc gttgatttcc
     5101 tctgcgctgc cgggtgcttg tcccataaac tgattgcaag ggaaatccaa tatttcaaag
     5161 ccctgatctt gatagcgttc atagagttct tgaagtccct ggtactgggg cgttaaacca
     5221 catccagtag cagtgttgac aatcaagaga actttgccac gatagctatc caagggagtt
     5281 gcttggttgt tttggttcaa aacggaaaaa tcataaagtg aagtcatgag ggcctctttt
     5341 ctttttctac attgtaacag tttttacttt tttcgtcgag tcggatggaa gatattgttg
     5401 aagaataagc cctcaatatg atagaaaccg tttacaaata actttaaaaa tggtataatt
     5461 taattaaacc ggtttactta gttgctataa atctctaatt tgagatttag ttcctattga
     5521 ttttacaata tgtttattgg agtgtataca tgcaaacaaa aacaaagaag ctcattgtga
     5581 gtttgtcttc acttgtttta tcaggatttt tattaaacca ttatatgaca gttggagcgg
     5641 aagaaacgac tacgaatacc attcagcaaa gccagaagga agttcagtat cagcaaaggg
     5701 atacaaaaaa tttagttgaa aatggtgatt ttggtcagac ggaggacgga agcagtccgt
     5761 ggacaggaag caaagctcag gggtggtcag cttgggtaga ccagaagaat agttctgcag
     5821 atgcctcaac tcgagtcatt gaggctaagg atggggctat cactatctca agccctgaga
     5881 aattaagggc agcggttcac cgtatggttc ctattgaagc taagaaaaag tataaactgc
     5941 gtttcaagat taaaacagat cataaagtcg ggattgccaa agttcgtatc attgaggaaa
     6001 gtggtaagga caagcgattg tggaattctg caacgacgtc aggaacaaag gactggcaga
     6061 ccattgaagc agactatagc ccgactttag atgttgataa aatcaagctg gagttattct
     6121 atgaaacagg aactgggact gtttccttta aggatattga gctggtagag gtagcagccc
     6181 agctttctga ggattctcaa acagataaac agcttgagga aaagattgat ttaccaattg
     6241 gaaaaaaaca tgttttttct cttgcggact atacttataa ggtagaaaat cctgacgttg
     6301 cttcagtcaa aaatggaatt ttagaacctc ttaaggaagg gacaaccaat gtcattgtca
     6361 gtaaagatgg caaggaagtg aaaaagattc ctttgaagat tctagcctct gttaaggatg
     6421 catacacaga ccgtttggat gactggaatg gcatcattgc tgggaatcaa tactatgatt
     6481 ctaaaaatga acagatggcc aaattaaacc aggaattgga aggaaaggta gctgatagcc
     6541 tatctagtat ttcaagtcag gcggaccgca cctatttgtg ggaaaaattt tcaaattata
     6601 aaatgtctgc aaatctgact gccacttatc ggaaattgga ggagatggcc aagcaagtga
     6661 ccaatccttc ttctcgttat tatcaagatg aaactgtcgt tcgaacagtc agggattcca
     6721 tggaatggat gcataaacat gtctacaata gtgaaaagag cattgttggg aactggtggg
     6781 attatgaaat cggtacacct cgtgccatca acaatacctt gtctctgatg aaagaatact
     6841 tctctgatga ggaaattaaa aaatatacag atgtgattga aaaatttgta ccagatcccg
     6901 aacatttccg aaagacgact gataacccat ttaaggctct aggtggaaac ttagttgata
     6961 tgggaagagt aaaagtaata gctggtttac tgcgtaagga tgatcaagaa atttcttcta
     7021 ccattcgctc gattgagcaa gtgttcaagt tggtagacca aggtgaaggt ttttatcaag
     7081 atggatccta tatcgaccat accaatgttg cctatacggg tgcttatggg aatgttttga
     7141 ttgatggcct gtctcaactg ttgccagtca ttcaaaagac caagaatcca atcgataaag
     7201 ataaaatgca aaccatgtac cactggattg ataaatcgtt tgctcctttg ctggtgaatg
     7261 gagagctgat ggatatgagt cgtggacgct cgatcagtcg tgcaaatagc gaggggcacg
     7321 tggccgcagt agaagtacta agagggattc accgaatagc ggatatgtct gaaggagaaa
     7381 ccaaacaacg tttgcagagt cttgtgaaga ccattgttca atcggatagt tattatgatg
     7441 tctttaagaa tttgaagact tataaggata tcagtttgat gcaatccttg ttaagtgatg
     7501 caggagtcgc aagtgttcca agaacaagtt acctatctgc ctttaacaag atggataaaa
     7561 cagccatgta caatgcagag aaagggtttg gatttggctt gtcactcttt tccagtcgta
     7621 ccttgaatta cgaacacatg aacaaggaaa ataaacatgg ttggtatacg agtgatggga
     7681 tgttctatct ttacaatggc gatttgagtc actatagcga tggctactgg ccaacagtta
     7741 atccatataa gatgcctggt acaacagaga cggatgctaa gagagcggat agcgatacag
     7801 gtaaagttct accgtctgct ttcgttggaa cgagcaaact agatgatgcc aatgcgacag
     7861 caaccatgga tttcaccaac tggaatcaag taatccatat aaagtctatg tcaatgataa
     7921 agaagcctcc cttacagaac aagaaaagga ttatcctgaa acccaaagtg tctttttaga
     7981 atcgtccgat tcgaaaaaga atattggtta ctttttcttt aagaagagtt caatcagtat
     8041 gagtaaggct ttgcaaaagg gagcctggaa ggatatcaat gaaggacagt cagacaagga
     8101 agttgaaaat gaatttctta cgattagtca ggctcataag caaaatggag attcttatgg
     8161 ctatatgcta attcctaacg tggatcgtgc caccttcaat caaatgataa aagagttaga
     8221 aagtagcctc atcgaaaata gcgaaaccct tcagtctgtt tatgatgcta aacaaggagt
     8281 ttggggcatt gtgaaatatg atgattctgt ctctactatt tccaaccaat tccaagtttt
     8341 gaaacgtgga gtctatacca ttcgaaaaga aggggatgaa tataagattg cctactataa
     8401 tcctgaaacc caggaatcag ctccagatca ggaagtcttt aaaaagctag agcaagcagc
     8461 tcagccacaa gtacagaatt caaaagaaaa ggaaaaatct gaagaggaaa agaaccattc
     8521 ggatcaaaag aatctccctc agacaggaga aggtcagtca atcttggcaa gtctagggtt
     8581 cttgctactt ggggcatttt atctattccg tagaggaaag aacaactaat ttgttcatca
     8641 tctagatgaa ttacatgaaa ctgtggaaaa tagaaaaaat ccgaagaagg ccaaatatct
     8701 tcggattttt cttttaaaaa aggataagtt gatacttatc cttttttact tcattattat
     8761 gtgactatag cgttttgaaa ctagaataga acgttataac tgctaaaaga tttctataaa
     8821 ttcatttgat tttcctaatc aatttgttcg tatcctattt cactccacta tataagtccg
     8881 tctcgctccg tgaggtgcgc gtcgaaggtt ataccaaaaa aactccaaac gcgatacaat
     8941 aaaggtattc aatccaattg taaagcgaaa ggagaaaaaa tatggcacaa aagactcata
     9001 gtttatcata cacaaagtgg atgtgttcta tcatattgtg ttcaccccta agtatagacg
     9061 aaaagtcatc tataatcaat atagaagtag tttaggcgaa atatttcatc gcttgtgtag
     9121 ttataaaggt gttgaaatga tcgagggtca cttaatgcca gaccatgtac acatgttagt
     9181 cagtattccg tcgaggctaa gtgtttcaag ttttatgggg tatttaaaag gcaaaagtgc
     9241 actcatgatg tttgacaaac acgccaatct caagtacaag tttgggaatc ggaatttctg
     9301 ggcggaaggt tattatgtaa gtccagtagg gcttaatgaa gccacaatta agaaatatag
     9361 tcaagattaa agaaattatc gagtggatga tttcttcacg agtatgaaaa tttgagaact
     9421 agtaaagtat gatatatagt aaaatgaaat aagaacagga caaaatcgat caggacagtc
     9481 aaatcgattt ttaacaatgt tttagaagta gaggtatact attccagttt caatctacta
     9541 tagcactaga taaattgagt gtaaaagaat atgaggatcc ctttagggat agtggtaagt
     9601 aataccaaag tctcttaaag aggcaagtga cgagtcaaga gcaataaggc ttgaacaacg
     9661 tgaaagccag cgtctttagg cgctggctga tgatttgggc ttatagctct gagataaacc
     9721 acccgttaga cgggtagtta tgattttatc tgagtgtaat atactgttgg gcaatctcgc
     9781 tgatgcggtc aaagttgcct tgggaagcga gtttattgag ttcgccacca attccaacgg
     9841 catctgcacc agcagcgaac cattgaggga tgttgtttag gccgactcct ccggttacca
     9901 ttacggaaac ttgtgggatc ggtgccttga ctgcagagat atatgctgga ctgagagtac
     9961 tacctgggaa gagtttgatg atttcactac cggcttcaag tgcagtcgtg atctctgtga
    10021 gggtaataca gcctggaatg tacggtgtgc tgtagagatt gcacattttc gcagtttcag
    10081 catggaaaga tggagaaaca acgtaatttg ctccagctag aatggcatct ctagcagtta
    10141 cggcatcaag cacagtacct gcaccgatac aaacactctg atcgtcctga tacaagtcta
    10201 caagttcctt gatgatttgt cctgcatact gattggtata ggcgatttca atagctttga
    10261 taccgccctt gatacaagca atcgaggctt gtagtccttc ttcctttgta tttcctcgaa
    10321 tgacagcgac aattttctgt ttttttagtt caataatcgt atctgatttg gtcatgtaat
    10381 tctcctaacg aatgatatct tgtgcatttg ccagtaaatt ttcaatacta gttgcggaag
    10441 tggagagatg gtctcctgga agagtgcatt tgagagttgc gctcgcaact gcaaagtcaa
    10501 tggtagtttt tagggaggaa tgatggagta gttggtaaag ggcaccagat ataaaggcat
    10561 ccccgctacc aattcgttga tagactgcag tttttagttg gacagactct tcaaatcgtt
    10621 cttccagagc ataggcttga tagacatttt tgtcttgctc atcactagat cggagggtat
    10681 ggaaaatggc cttgaaacca taggcttctt ttaaaagtcg catgcgattt tccacctctt
    10741 ctaggctagc actgtctctt ggaaacatct ctagattttg gtcatcaatc atgagaggat
    10801 caataccgaa gcaatagtca gtaaaacgtg caaacttaga aaattcatac ttggcttcta
    10861 ggactgaaat catctttgtt ctcagattga gatccattga aacgacaatt cctcggcgct
    10921 tggcttcttc caagagtagg agaaggatcg cacggacctc ttgaccgata gctatggtga
    10981 ttccactaaa atgaaaatgg ctaatcccct gaaagagaga atccatatct agcatgtttg
    11041 gccgaatctg gctgatactc gtatgcttac gatcgtagaa aacttcactt tgacgacaac
    11101 caaagccgtt ctccaaatag tagaggccga ttcgatcgcc aagccgacag attgaactgg
    11161 tatcgatttg gtgctgtttc aagaatgtga gaaaacgatc tccaatctcg ttggcaggga
    11221 gtgcggtaaa gactttcgtt gagataccca gggcttgcaa attacaagcg atgttaattt
    11281 ctgatccgcc aaaataagtc gaactggcaa catgatcgcc gatactggtg gcatctaatg
    11341 gtgagattcg aattaatggt tcgccaaaca gtaatatttt cgtcatccat tagttccttt
    11401 ctggtttaga atagctttac ttggctaata cttctttcaa ataagcagcg acttcatcat
    11461 cttggcagtt ttcaaagaag tattcttgga agtatttgcc agagatggtg tctttgagga
    11521 agtcttggtc gattgtttta aggatggtca tcaaatcatt gtgggtgatt tgtttcacct
    11581 cgtttaagat acgagcgtta cgttgttcag ggattacacg ttctcttggg tagccaccgc
    11641 ccatttcttc agcaaataag cgttcaaaca tcaatttgag ggtcagttct gccccccagc
    11701 caaatccttt ggcataaggg atagacaagg cgttaccacc attgatttga gaataaaggt
    11761 aagcgtcagt tgggtccact gctagaccac agacaacacc agggaagctg tttaaagcaa
    11821 gcatagcccc tacacccgta ccacagccgg taacaacaaa gtcaactgcc tttgtattta
    11881 aaaggatggc agccattagt ccgttctgca cataagttaa ttgactttct ccttcttctc
    11941 cacgcatacc atagttaaat aattggtagt cttttttatc tgtcgcttct tttagactat
    12001 cgtaaataat gtgattcttg ctagcttgac tattttcatt gattaatgcg attttcataa
    12061 gatcttcttt ctatttttac tcaggttgtt ttccgatgta ggctaagata ccgccatcta
    12121 catataggat gtggccattg acaaaattgc tggcatcact agcgagaaag acagcagggc
    12181 ccatcaaatc ttcaggattt ccccaacgtg cagcaggtgt ttttgcaatg atgaactggt
    12241 caaatgggtg gcgagaacca tcttcttgca actcacgaag aggtgctgtt tgaggagtgg
    12301 caatataacc cggtccaatt ccgttacatt ggatattggc tccaccgtat tcagacgcaa
    12361 tgttgcgggt caacattttc aagccccctt tagcagcagc ataagcgcta actgtttcac
    12421 gtcccagttc gctcatcatc gaacaaatat tgataatctt tccatgccct ttctttatca
    12481 tagaaggaat aactgccttt gaaacgataa atggtgcgtt taagtcaata tcgatgacct
    12541 tacggaaatc agcggcgctc atttcgcaca ttggaactcg gcggataata ccagcgttat
    12601 taacgaggat gtcaatgaca ccaacctctt gttcgatttg cttgaccatg gcttggatac
    12661 cgtcctcgtc tgtcacgtca cagacatatc catgggcttc gatgccaact tcacgataag
    12721 cttcaatccc tttattgacc agatcttgat tgatatcgtt aaagacaata gtagcgccgg
    12781 cttcagcgta ggatttggca atagcaaatc caattccata agaagcgccg gtgatgagag
    12841 caatttttcc ttttaaagaa aactgctcaa ttgagaatga tgtatttgtc atttgatact
    12901 tcctttcaaa tttgttaaga cttctctata accaaattat attctagtaa acc
//
LOCUS       DAVLYR010000044        12487 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLYR010000044 DAVLYR010000000
VERSION     DAVLYR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.044"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(388..1614)
                     /locus_tag="VYO63_000966"
     CDS             complement(388..1614)
                     /locus_tag="VYO63_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1671911.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFKNEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1657..2349)
                     /locus_tag="VYO63_000967"
     CDS             complement(1657..2349)
                     /locus_tag="VYO63_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1671912.1"
                     /translation="MLKWEDLPVEMKSSEVESYYHLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(2522..4273)
                     /locus_tag="VYO63_000968"
     CDS             complement(2522..4273)
                     /locus_tag="VYO63_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671913.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDDQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(4263..6008)
                     /locus_tag="VYO63_000969"
     CDS             complement(4263..6008)
                     /locus_tag="VYO63_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671914.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6136..6666)
                     /locus_tag="VYO63_000970"
     CDS             complement(6136..6666)
                     /locus_tag="VYO63_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1671915.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(6663..7119)
                     /locus_tag="VYO63_000971"
                     /pseudo
     CDS             complement(6663..7119)
                     /locus_tag="VYO63_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7253..8080
                     /gene="xth"
                     /locus_tag="VYO63_000972"
     CDS             7253..8080
                     /gene="xth"
                     /locus_tag="VYO63_000972"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1671916.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8092..8190
                     /locus_tag="VYO63_000973"
     CDS             8092..8190
                     /locus_tag="VYO63_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1671917.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            8235..8474
                     /locus_tag="VYO63_000974"
     CDS             8235..8474
                     /locus_tag="VYO63_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1671918.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      8857..8952
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9352..9933
                     /locus_tag="VYO63_000975"
     CDS             9352..9933
                     /locus_tag="VYO63_000975"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1671919.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            9933..11195
                     /locus_tag="VYO63_000976"
     CDS             9933..11195
                     /locus_tag="VYO63_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1671920.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11245..11706)
                     /locus_tag="VYO63_000977"
     CDS             complement(11245..11706)
                     /locus_tag="VYO63_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1671921.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(11703..12467)
                     /locus_tag="VYO63_000978"
     CDS             complement(11703..12467)
                     /locus_tag="VYO63_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1671922.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYYPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
BASE COUNT         3647 a         2730 c         2479 g         3631 t
ORIGIN      
        1 attctatcat gcaaaagaat gactagaaca tgaaagacag gcacaaaact ttgaacaata
       61 ttctgaaaac aaaaaaagac gcgagtttcc acaatctcac tcatggaaac tcacgtcttt
      121 caaaaaatag ggtgaattga aaactagaac aagacactag attttctaaa tgatgtctag
      181 taaggattct aaatgctaca ccaaattgct cacatcttat ttcaaaccac gggactctca
      241 caagaaagga aaatcctata aaaattctct ctctgaggag aaagaggcct caactacagg
      301 tgtaaactat tatcaattga aaacaacagt ttttacactc aattagactt tttagggaca
      361 ggatcacttt cgcttgactg tagtttgtca tttttttgat aaagttagca ctttttcaga
      421 aactgttttg aaagtctcaa tgatatagtc tacttcttca tcgcttaatt tagtatgaag
      481 agggagggta atttcattct taaagaaggc ataggcctta ggatagttcg tcatatcaaa
      541 tccaagattc ttataggctg tcaagagagg aagcggtttg tagtgaacat tacttgcaat
      601 tcctgcttta gccaattctt ggatgatgag gttgcgttct tctaggcttg ctccttctac
      661 acgggtgatg tagaggtggc gtgaagattc gacagtttca gtcttgtgtg ccaaaggatg
      721 gatgcgagaa cctgcaaaac cactatcata gcggtccaca atgtccttac ggcgttgcaa
      781 caaacttgga tagcggtcca attgtaccaa accaagtgaa gccatgatat cggtcatgtt
      841 gcacttatag gctggtgtaa cgatatcgta ttcccatgac cccagttgca tcttggcaag
      901 agcatcctta gtttgcccgt gaagggaaag gatttggaat tccttgtaca tctcttcgtc
      961 atcaatcact ggattggctt tccaagtcgc acttccacct tctgccgttg taaagttctt
     1021 aacggcatgg aatgagaagg aagtaaagtc agcgatagaa ccagaaggtt gtcctttata
     1081 agtagatccc aaagcgtggg cactatcaga gacaatgaca atacggttaa aggccttttg
     1141 ccacttgctt gaagcagtaa agaagtcacg ttttttctcc acgacttgga acaaacggtc
     1201 ataatcgcaa acaatccctg cgagctctac tgggataatc accttagttt tctcagtgat
     1261 agcttgctca agcaggtcat agtccatctc aaacgtatct gcttggatat ccaccatgac
     1321 aggggttgct cccacgtgcg taatgacact acatgaagct gtataggtca tggctggaac
     1381 gatgacttca tcaccaggtc ccacttccaa aacgcgtaaa atcaactcca gagcggctgt
     1441 cgcagagttg agacaaacag tcttaggtgt ctgtgtgtaa agagacaagc ggcgctccag
     1501 ttcttttgtt ttaggacctg ttgtgatcca accagaacgc agggtatccg ctacttcagc
     1561 aatttctgct tctgtgatat caggcggtga aaatggaata ttgtaatttg gcattgattt
     1621 gctcctttta tctgtactca ttttcttatg actactttat tttagtacct caaacacggt
     1681 ttgaaacatg attttgatgt ccccaaagaa actaaactct cggagatagg cgaggttata
     1741 gcgcatcttt tcaggaagaa catgctccac ataggcctga tcaactgaca gacctttctc
     1801 cgtcatttga ctgatgatgg tgtcctcatc cttgtagttg atgctggctg gagaggtaat
     1861 tcctgcttgc aagagcaagg ttgccatcat ttcagggcta tactgctctg tataacgtgg
     1921 cacttcaggt cgtgtaccga caaaggacat ctcaccttta aggacattga ccaactgagg
     1981 cagttcgtcc aaacggacac gtcggatgaa atttccaacc ttggtaatac ggctatcgtt
     2041 agcagaagtc accagacttc cttttttatc cgcatccgtc accatggtac ggaacttcca
     2101 aatcttgaac cgacggttgt actgggtcac acgctcttgc ttgtaaatca ctggcccctt
     2161 gctatccaac ttgatccaaa tgctcaagat gagaaagatg ggagaggtta gaaccagtaa
     2221 gaccaaggcc aaaaaccagt ccaagcaacg cttgaaaatc agcgaaccct tccttttaga
     2281 gacaagatgg tagtaagact caacctcgct tgatttcatt tccacaggca agtcttccca
     2341 tttcagcata ctgttctcct ttgtttttat tatactattt gtcaacattt ttcattatac
     2401 cacaaaatgg aaaaggcggt gaaagaaatc tgtaatttga aggatttcct tctagtactc
     2461 aatgaaaatc aaagtacagc tttgagattg cagataaagc tgacatggtt taaagagatt
     2521 ttcaaagagt atcggccatg gcccctgcct gcaaactata catcttgtga taggttcctc
     2581 ccagagtcaa gagttcctca tgggttccac tctcgataat gcgtccctta tccaagacat
     2641 agatgcagtt ggcgtcttga atagtagaaa ggcggtgagc gatagcaata gttgttcggc
     2701 cctgtctcat cttcgccaga gaagcttgaa ccaagctttc tgtttcagag tcaatattgg
     2761 ctgtcgcttc atccaaaatc aggattttag gctggctggc gactgttcta gcaaaggcaa
     2821 gaagctggcg ctgcccagta gagaagctcg aaccacgctc ggaaacaggg gagtcgtacc
     2881 cctgaggaag ttcttgaata aaggaatctg catccacaaa ggctgccgca gcctgaacct
     2941 gatcatcact gatttcttgg tacatggcga tattggactt aatagttcca tgatagagga
     3001 agggttcctg caagaccaaa ccgatgtttt ttctcagctc ttcttgactg aaatccctga
     3061 tatccacatc atccaagaga actctccctg actggaattc ataaaagcgc atgaggacat
     3121 tgataataga cgatttccct gaacctgtat gacctacaaa ggcaatggtt tcacccttat
     3181 taacagagaa agaaatgtca tccagaatcg gatgtttacc gtcatatgag aaacacacat
     3241 gttcaaaacg gatattgcct tcttggactt tggcttgccc attttcttga agaggttcat
     3301 aggtcctctc gtctatcagg gcaaagacac gacctgcaga aaccatagcc gtttgcagag
     3361 ttgaaaagtt ttgcgtcacc tcaatcaagg ggtcaaaaag gcggttgatg tactggataa
     3421 aggcatacat ggtcccgacc gttatcccga tagaaaaacc acggtagcca aagtaggcca
     3481 tcaagactgc atagcctaga agtttcagca aactcatggc aggtctcaaa aagagggcat
     3541 ccaaggctac agaacggttg gcgtagacca agtgttcttg gttgatttca tcaaattctg
     3601 cctgcaggcg cttctcttga ttaaaagcct gaataatcct gattccctcg atattctctg
     3661 ccagcttact attgatatct gacaagagac ttctggtttt ctcaatgatt ttcactgatt
     3721 ttttccgata gagattgacc aaaaggaaaa tcaaggggag aaagagcaag actaatgctg
     3781 tcaaacgaaa atccaatacc aacatggtat aaagggttgt cagaaagatg aaaactgctg
     3841 agataaagct ggataaaatc cccgaaaaca tatcactgat agtctcggtg tcatttgtca
     3901 aacgagagac gatagagcct gctggcgtct tgtcaaagta agacatgccc agtttctcca
     3961 tattggcaaa ggcatcccga cgaatatccc taacaatact gtaagacacg cgcgcaaaga
     4021 gaagattgcc tacatactga actacagttt gtaggatgta gagaccatag tagaccagca
     4081 aaacggtaac ggctagttgg ttaagattgc tgagatactg gtcgataaag tgggaagcca
     4141 cgaggggtat gacactttta atgaccgtcg tcgctaggag aaaactgagt gccaaaaagg
     4201 tcaggagtct ataaggcttg agataagaca tcaagcgctt caatacagcc cattgttctt
     4261 gtttattctg catcttcttc tcctttcatt tctaactgct gagactggta ggtttgggca
     4321 taccagccat ccatcgctag caagtcttcg tgcgtgcctc gttcgataat ttgaccattt
     4381 tgtagaacta aaataaaatc tgcatggaca acagcactga ggcgatgggc agtgataatg
     4441 gttgtcttgt cctttcgcgt ctccttgagg ttgtcgataa tcgcatactc tgtcttggca
     4501 tctacggcgg ataaggaatc atccaaaatc aagatatcag ggtctaaaat catagcccga
     4561 ctcatagcca accgttgctt ttgaccacca gaaagactga ctcctttttc accaatcagc
     4621 gtatcaaatc cttgaggcat gtctacaata tcttggtaaa ctcgggctag cttagtagct
     4681 tcctcgaccg ctgaaagggg caagttagga ttgccaaagc ggatattgtc taggattgaa
     4741 gttgcaaaga gaaactggtc ctgaggaaca tagcccatga gactgcgaag gtctgtcaga
     4801 cgatagtccc gaatatcgtg accgtttaga taaatggcac ccttatccac atcgtattca
     4861 cgcaagagga gcttgattaa ggacgttttc ccagagcctg tctgcccaac caagcccagt
     4921 gtttgccctt ttgccaaact aaagtgaata tccgtcagtg tttcctcatt ttcaaaagca
     4981 aagctgtcaa tggcatactc caaacgccca ttttcaatac catccagagg aaactcaggg
     5041 tcttgtacag gagattcctg agacaaaaga ttttcaatcc gctggtaaga aaccttccct
     5101 cgctgagtag tattaaagag gaagccgatg gccataagag gccagaccag catatccaaa
     5161 tagctgataa aggtgactag attcccaact gtaatctgcc cttcctgaac catcaaggag
     5221 ccaaccaaaa gcgttaaaac ataggacgaa ccaacaaaca cgagaaccat agggtcaaag
     5281 agactatcat atttcatggt ttgcaggttc ttttggaagg ttaattcatt gactgcctga
     5341 aaagacttca actcgtctgc ctgataaccg aaagacttgg tcactttgat acctgatacg
     5401 gactcctgta ccttgttatt gagttcagaa aaagcagctt gggattcgcc aaaggcctta
     5461 tgagtctttc tccctaggcg actagtcgca taggccatga aaggtagggg gagaatggca
     5521 acaagagtca tctgccatga gatgctaaag agcatggtca acaaagtcac cagagccgtg
     5581 atagaggcat ccaccgcaga catgacaccg ccacctgcta aacgagtcaa ggcattgata
     5641 tcattggttg cgtgtgccat cagatcaccc gtccgatagg tttgataaaa ggctgatgac
     5701 atttttgtga aatgcttaaa caagcgagac cgcatgatct gtcccaagca ataagaggtc
     5761 ccaaggatat acatacgcca cacatagcgc aaatagtaca taccaaaggc tgcaagtagc
     5821 aagtaaaata ggctaagaag gaggtcctgc tgggttaatt gccccgatgt gatggcatca
     5881 atgacccgcc ccataaccat aggaggaatg agattgagaa cggaaaccaa gaccagggcc
     5941 acaatcccga ctagataacg gcgtttttct aacttgaaaa accaccaaag tttttgaata
     6001 atggacataa aatccctttc tgattgcaaa tggaaacctg aggccaatac tcaatgaaaa
     6061 tcaaagagca aactaggaaa ctagccgcag gctgctcaaa gcactgcttt gaggttgtag
     6121 atagaactga cgaagtcagt aacctacata cggcaaggcg acgttgacgc ggtttgaaga
     6181 gattttcgaa gagtacaaga ccccaggttt ttctttttca taagttacga ctgtaacagc
     6241 acccttgtca tattcagcaa taaagatatt ggctacattg tcatgccctt gtttactgag
     6301 gttatcaagc aaccactctt cgctacgacc aatcgattcc aagacatcta cttgaatcac
     6361 accgtcagtc acaactggat acttaggatt ttcatctccc atttgcacaa cgataagttg
     6421 cccattttgc tcttgcacag ctcgtttgac ttgcttcatc tggaaaatcc cttggctacg
     6481 aagtttgagg gctacatccg atgcagacaa accaactgaa cgacaggctt ctgggtcaat
     6541 attcccattt ttgataagga gagttggctt accatcaatc aaacgtttca caaagtgaac
     6601 attgttgttg agccatttga gagtcaagac cagtttgatt aaaatttcga tataattaag
     6661 tgtcatttgt tcacatccac cagttcaatt gcgtctgtct tatacaattc gactttctct
     6721 aacaggtact tgtctggctc acttccgttt agggcacgat aatagcgatc tcccaccttg
     6781 ataagtgcgc catctgtgtt tgtggaagta ttaacataga cttctgactt gtctactccc
     6841 aaatctttgg aaacaacctc gatgaaatga agtgaagttt gaaatttatt gttagaaact
     6901 tgattgtttt gataagtcgt gatgctgacc aaaagcagag ctaaataagg ccagagctga
     6961 aatcatgacc aactcacgaa attcggttcc ctttttatca tggtatgcct tgaaagcaaa
     7021 aaaaactgtc acagctaata gaacaatccc aaagccaacc atgataccat tttgctgacc
     7081 gatttggctg agtacatagt cataagagta gaatttcata tattttcact ccctttcata
     7141 aaatcattct taattataaa cgaaagagag taatttttca aaccatacgt tggggaaata
     7201 gatctttttt tggtataata aaatctataa tctgaatgaa aaaggtaact ttatgaaact
     7261 tatctcatgg aatattgatt ccctaaacgc tgccctaact agtgactcag ctcgtgccaa
     7321 attgtcccaa gaagtcctac aaaccttggt cgctgaaaat gctgatatca ttgctatcca
     7381 agaaaccaag ctttctgcca aaggacctac aaagaaacac gtggaaattt tagaagaact
     7441 cttcccaggc tacgaaaaca catggcgctc ttcccaagag cctgcccgta aaggctatgc
     7501 tggaaccatg ttcctttata agaaagaact tacgcctacg atcagttttc cagaaatcgg
     7561 tgccccttct accatggact tggaaggccg tatcatcact ctagaatttg atgcattttt
     7621 cgtaacccaa gtttacactc caaacgctgg tgacggtctc aaacgcttgg aagaacgcca
     7681 agtctgggat gttaaatatg ctgagtattt ggctgaacta gacaaagaaa aaccagtcct
     7741 tgcgaccggt gactacaacg tagcccacaa tgaaatcgac cttgcaaatc ctgctagcaa
     7801 ccgccgttca cctggattta ctgacgagga acgtgctgga tttaccaacc ttttggcaac
     7861 tggatttaca gacaccttcc gccatgttca tggcgatgtt cctgaacgct acacttggtg
     7921 ggcacaacgc agcaaaactt ctaaaatcaa caatacaggc tggagaatcg actactggct
     7981 cacaagtaac cgtatcgctg acaaggtcac taaatctgat atgattgact caggtgctcg
     8041 ccaagaccat acaccgattg tcttggaaat tgacttgtaa ggattttcta tatggactac
     8101 aatgcggtca ttcccgagtt tcttgtatcg aatatcgaac agtcccggtc cttctactgt
     8161 ggtttactgg gttttaggat tgaatactaa cgtccagaag aaaactttct ctttctcttg
     8221 aagagtgtca actaatgtta gaggagggaa caaaggatca actagctgaa ctgacctacc
     8281 cctttggtcg tggagtcaat ctctcctttg gcataaagga tgttccaaaa ctctatcaaa
     8341 aagtgatgga ggcaaactat cctatttatc gtctcttgac caaaagaaag tttcgtgtca
     8401 gtgatcccta tatctatcct cataaatttg cagttttgga tccagatggc tattttttaa
     8461 gatttagcga gtagaataat aaaaattggg aaaagtcttt aaagttagaa aaaggcatat
     8521 tgtcaggtgt ctaaaaactt gatagtatgc cttttatgat ggaaaaatct tctaatactc
     8581 ttcgaaaatc tcttcaagcc acgtcaacgt cgccttaccg taggtatatg atactgactc
     8641 tgtcaattct atccacaacc tcaaaacagt gttttgaggt tgtggatagc ttcctagttt
     8701 gctctttgat tttcatttag tataaatgtt agcttacaat gaagtttttg ttcaccctct
     8761 cttctttttg ttcgcttttt aaaccaaaac actaacaaaa tagttgattt ctaaagttat
     8821 ttagtgtaaa ataaaaacat tggctcaagc ctatttaaaa ttgaatatcg ttttacttgt
     8881 ttatgtcgtg aattggcacg acgtttctac aaggtgccgg aacacctaac aataagtaag
     8941 tcagcagtga gatatggccg tttttgccat gtctatttag gggacttact tagagattaa
     9001 gtaggtcttt tttgaatatt tttaatattt cccttaatta gtgcggacgg gaggtgaaat
     9061 tatcctgtga ataatttcag gaatccaggg aatttcatcc ctaatttttg agcccctagc
     9121 tctttcattt ctaggcttag gctaaaatag ttccccgaac tattttactc tcaaaaatcc
     9181 cgtcatagac aataacaaga ttattgtcta tgataccact acaacgggaa atatttgata
     9241 agactcgctt cgctcgtaat agttttatca aatatatagt attagcaaaa gggatcacta
     9301 tagaaatatt taaacaacaa gtaaattaac ttttagactt taggaggtct tatgaaatta
     9361 ttagaagagc gcatcctcaa ggatgggcat atcttgggtg ataacatcct caaggtagat
     9421 tcctttttaa cccaccaagt tgactttagc ttgatgcgag agattggtaa ggtttttgcg
     9481 gaaaaatttg ctgctactgg cattaccaag gtcgtaacca ttgaagcgtc gggtattgcc
     9541 ccagccgttt ttacagctga agccttaaac gttcccatga ttttcgccaa aaaagctaag
     9601 aacatcacca tgaacgaagg catcttaact gctcaagtct actcctttac caagcaggtg
     9661 accagcactg tttctatcgc tggaaaattc ctctcaccag aggacaaggt tttgattatc
     9721 gacgatttcc ttgctaatgg ccaagctgct aaaggcttga ttcaaatcat cgaacaggcc
     9781 ggtgccacag tccaagctat cggtatcgtg attgagaaat ccttccaaga tggccgtgat
     9841 ttgcttgaaa aagcaggcta ccctgtccta tcacttgctc gcttggatcg ttttgaaaat
     9901 ggtcaggtcg tatttaagga ggcagatctc taatgcaaac tcaagaaaaa cactcgcaag
     9961 cagccgttct gggcttgcag cacttactag ccatgtactc aggatctatc ctggttccca
    10021 tcatgattgc gacagccctt ggctattcag ctgagcagtt gacctacctg atttctacag
    10081 atatcttcat gtgtggggtg gcaaccttcc tccaactcca actcaacaaa tactttggga
    10141 ttggactccc agtcgttctt ggagttgcat tccagtcggt cgctcccttg attatgattg
    10201 ggcaaagcca tggtagtggc gctatgtttg gtgcccttat cgcatctggg atttacgtgg
    10261 ttcttgtttc aggcatcttc tcaaaagtag ccaatctctt cccatctatc gtaacaggat
    10321 ctgttattac cacgattggt ttaaccttga tccctgtcgc tattggaaat atgggaaata
    10381 acgttccaga gccaactggt caaagtctct tgcttgcagc tattactgtt ctgattatcc
    10441 tcttgatcaa catctttacc aaaggattta tcaagtctat ctctattttg attggtctgg
    10501 ttgttggaac tgccattgct gctactatgg gcttggtgga cttctctcct gttgcggtag
    10561 ctccacttgt ccatgtccca actccactct actttgggat gccaaccttt gaaatctcat
    10621 ctattgtcat gatgtgtatc atcgcaacgg tgtctatggt tgagtcaact ggtgtttatc
    10681 tagccttgtc tgatatcaca aaagatccaa tcgacagcac gcgccttcgc aacggttacc
    10741 gcgcagaagg tttggccgta cttctcggag gaatctttaa caccttccct tacaccggat
    10801 tttcacaaaa cgttggtttg gttaaattgt caggcatcaa aaaacgcctg ccaatctact
    10861 acgcagctgg tttcctggtt ctccttggac tgcttcctaa gtttggcgcc cttgcccaaa
    10921 tcattccaag ctccgtcctc ggtggtgcca tgctggtaat gtttggtttt gtatcaattc
    10981 aagggatgca aatcctcgcc cgtgttgact ttgctaacaa tgaacacaac ttccttatcg
    11041 cagctgtttc aatcgctgca ggtgtcggtc tcaacaacag taatctcttt gtcagcatgc
    11101 cgacagcctt ccaaatgttc ttctcaaacg gaatcgtcgt agccagccta ctcgctattg
    11161 tcctcaatgc cgtattaaat cataaaaaga aataagaaaa agaggtgcga gcctcttttt
    11221 tgttgacaaa ataaaaacat ctacctattc atatatgcta gtgtcatagt cctgaaaatc
    11281 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc
    11341 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt
    11401 atactttttc agttttagat tgctttccaa taattcacct attaacaata atctatgata
    11461 ggagctatgt agccactctg gcaatttatc tcctacttcc cacccatatt tttccttcat
    11521 caaagctagt tgtttttcgt caccaaattt catattttca gataaccatc tcacacaatc
    11581 gggatctgat aaggtacaga cgattaaact taggttatcg taacggcttt cagtttcata
    11641 atcaggccag atgactttta agaaatcata cacctcttct gatgcaacaa gttgtttcgt
    11701 tttcataatt tccgatactt tcctctacct ttaaattcta ttatttcttt gtctcttaat
    11761 atttgaagct gttgcctaat cttttctttg atatgattgt tcttaacaaa gatatttttt
    11821 aggtcacttt caaaacgata catatcttca agggtaaatt ctgaaccctc tatcttatct
    11881 atacaattta gaatttctat tgtccaacct cttgatgaca gagagctctt ccttaaaaat
    11941 aaaccttgct taaattcttt tgtaactttt tctggatctc taacttgtcc atcttgcaca
    12001 agaaatatcc ttcctttaga aggtacttgt gataaatcaa tgttacaacc aatccaacct
    12061 gctcgtctag cagttggtgc aagtggtttt ctttgaataa tcgatttcgg tgtaacaaat
    12121 tgcttcggaa ggacaagaaa gttatttacc tcaaaatttt ttgtgtaagt taaaaaaaag
    12181 aaattaggat tattatctgc ctgcacacgc ttcatcatcg ttgcataagc accatcattg
    12241 attgttgatg aaaaatttcc ttttttgctc tttagttcaa actcctcact acaatgatta
    12301 cagtaaaaat ctgctacagg ccgattattt tcaaaatgat ttaagggatt gttcccacaa
    12361 ttagggtaat aagactgacg ataaacccaa tcctccgtta agatacgtgc cttttggcta
    12421 ttacttttat aggtttctac taattctaaa ttaaagtgta attccattta aaaattcctc
    12481 actaata
//
LOCUS       DAVLYR010000045        12423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLYR010000045 DAVLYR010000000
VERSION     DAVLYR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.045"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..1140)
                     /locus_tag="VYO63_000979"
     CDS             complement(<1..1140)
                     /locus_tag="VYO63_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1671923.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKEDIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQI"
     gene            1198..2364
                     /locus_tag="VYO63_000980"
     CDS             1198..2364
                     /locus_tag="VYO63_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1671924.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            2503..2829
                     /locus_tag="VYO63_000981"
     CDS             2503..2829
                     /locus_tag="VYO63_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1671925.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            2816..3409
                     /locus_tag="VYO63_000982"
     CDS             2816..3409
                     /locus_tag="VYO63_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1671926.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3402..4313
                     /locus_tag="VYO63_000983"
     CDS             3402..4313
                     /locus_tag="VYO63_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1671927.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4376..5440
                     /locus_tag="VYO63_000984"
     CDS             4376..5440
                     /locus_tag="VYO63_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671928.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5618..6478)
                     /locus_tag="VYO63_000985"
     CDS             complement(5618..6478)
                     /locus_tag="VYO63_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1671929.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFERVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(6753..7739)
                     /locus_tag="VYO63_000986"
     CDS             complement(6753..7739)
                     /locus_tag="VYO63_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1671930.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEEREQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINTLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(8011..9486)
                     /locus_tag="VYO63_000987"
     CDS             complement(8011..9486)
                     /locus_tag="VYO63_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1671931.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(9696..10619)
                     /locus_tag="VYO63_000988"
     CDS             complement(9696..10619)
                     /locus_tag="VYO63_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1671932.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10633..11562)
                     /locus_tag="VYO63_000989"
     CDS             complement(10633..11562)
                     /locus_tag="VYO63_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1671933.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGTLWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11881..12101)
                     /locus_tag="VYO63_000990"
                     /pseudo
     CDS             complement(11881..12101)
                     /locus_tag="VYO63_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12055..12315)
                     /locus_tag="VYO63_000991"
     CDS             complement(12055..12315)
                     /locus_tag="VYO63_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671934.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         3676 a         2669 c         2274 g         3804 t
ORIGIN      
        1 tatttggctt attttatcca aataaattat tgttctcttg tactccttct tccatttaaa
       61 tcctctaatt aaacctacaa tcgtcccaaa gccataagca aagtgaatgg aaaataaaag
      121 aaagggcatc acaattagaa atccattttt atgttttaat aaagtcagca aagtgagtaa
      181 tgacaaaagt agaaaatagg cacctaatag taaagttatg aatacgaatg tgatcggtaa
      241 caatgctaga ctaaacacaa gactcaaaac aaataaacaa ggaacatagt gaaataatga
      301 taaacactta aactgaacat gacttgtcaa gccaatccac aaaccatttg aatacttttg
      361 atgcagcatt ttcttgaatg ttggtcgaat atactgataa gatagaatac ttgggctata
      421 gcggatttta taaccatgtt ctcgaattct ataatgaata tcattatctt cagttcggcc
      481 aagttgctca tttactaaac caaccttctg gaaaacctct cgtttataca tcccatgaaa
      541 aatagaagaa acatatctat cctcggaact atttcgataa ttggcaatgc tactgccaaa
      601 catattttcc tcaacaagat gcaaggtctc tgcccatttt ccttttcctt cgacaatcgt
      661 cggtctaggc cccccacaga caaattcacc ttgttgaata atagccacat tgttcattac
      721 aaaactctca gtaacttttg aatgagcatc aatttttaaa ataaggtccc ctacagaatg
      781 tttaactccc aggttaaaac cactagcttg atttttctta ggattgttat acaatctaat
      841 tgagttaaac tctgtatctt cctttataaa ttgctgaatg atagctgtgg tcccatctgt
      901 ggacatagca tttataaata gaatttcaat atcctcttta ggataggttt gattttttaa
      961 gtcttcaatt agaccaggca gatatttttc ttcattgtat gcactgatta caatggatac
     1021 gagaagactt tcaacatttt gagtcactac tactctcctg aatttttcct tccaccattc
     1081 tatcataatt ttcaagactt tcccattttt atcttgaaag ccagaaacct tacttgacat
     1141 tatagtgaaa aagccttaaa ataagctgtt attaaaatca gctagaagag gtattatatg
     1201 aaaaagcaat cactcttttt tgttccaggt attatcctga ttggtgtttc cttgcgaact
     1261 ccttttactg ttttacccat tattttggga aatatttcgc aaggtctgga ggtagaagtt
     1321 agttcgcttg gtgtcttgac cagcctgcct ctccttatgt ttaccctctt ttcaccattt
     1381 tctacccaac tggctcagaa aatcggcttg gagcatctct tcacctacag cctcttcttc
     1441 ttgaccatcg gctcacttat tcgactaatc aatctgcccc tgctctatct aggaaccttg
     1501 atggttgggg caagtgtcgc agtcatcaat gtcctgcttc ctagtcttat ccaagccaat
     1561 caaccaaaga aaattggttt tctgaccacc ttatatgtaa cgtctatggg gattgcaacg
     1621 gctctggctt cctatctagc tgtgcccatt acacaagcca gttcttggaa aggacttatc
     1681 cttctcctca cgttactctg tctagcaact tttttggtct ggctcccaaa tcaccgctat
     1741 aatcatagac tagctccaca aaccaaacaa aaaagtcaaa taaaggtcat gcgtaataaa
     1801 caggtttggg caattattat cttttcaggt tttcaatcct tgatctttta caccgtcatg
     1861 acctggttac ctaccatgtc tatccatgca ggtctatcca gtcacgaagc tggcttgctg
     1921 acttctatct tatctctgat tagcattcct ttttcaatga ccatcccaag cctgacaacc
     1981 agtttatcta ctcgcaaccg tcagctcatg ctcactctgg tttcactagc tggtgtggtc
     2041 ggcatttcca tgctcttttt cccaatcaat aatttcattt actggcttgc catccatctc
     2101 ctcatcggaa ccgcaaccag tgccctcttc ccttatctca tggtcaactt ttcactcaag
     2161 acaagcgccc ctgaaaagac agcccaattg tccggcctat ctcaaacagg aggctatatc
     2221 ctagcagcct ttgggccaac cctctttggt tacagttttg acctgttcca ctcttgggta
     2281 ccatctgtag ctgccctctt gctcatcgat atcctgatga ctgtggccct ctttacagtg
     2341 gacagagcgg ataagatcct ttaaacttct agttttagct agaagttttt tatatataaa
     2401 aattttacac atttctcttg acagggcttt cttttagatg tacaatgtat gtgtagaaaa
     2461 attatatata aaaatcttac acattagaaa aggaggtttc ccatgtactt tccaacatcc
     2521 tctgccttga ttgaatttct catcttggct gtactggagc agggtgattc ttatggttat
     2581 gagattagcc aaaccattaa gctgatcgct aatatcaaag aatccacact ctatcccatt
     2641 ctcaaaaaat tggaaggcaa tagctttctg acaacctact ctagagagtt ccaaggtcgc
     2701 atgcgcaaat actactcctt gacaaacggt ggtatagagc agctcttgac cctaaaagat
     2761 gaatgggcac tctatacaga caccatcaat ggcatcatag aagggagtat ccgccatgac
     2821 aagaactgaa tacctgactc agctagaact ctatctcaag aaactacctg aagctgaccg
     2881 tatcgaagcc atggactatt tcagagagct ctttgacgat gctggagtcg aaggagaaga
     2941 agaactcatc gctagtttgg gaactcccaa agaagcggcc cacgaagttc tatccaatct
     3001 tctcgataaa aaaatcaatg aagcacccgc tcaaaaaaat aaccgacaaa ttttacatat
     3061 cgccttgtta gccctccttg cagcacctat cggcattcct ctgggaatcg ccatcctcgt
     3121 gaccctgttc gcaattcttg tagccgcttt gactgtcatt ctggctttct ttgcagtttc
     3181 catactgggt atcatcggcg gattcctatt tttagttgaa agtttcactg tcctcgccca
     3241 agccaaatca gcctttatct tgatttttgg ttctggttta ctggctatcg gtgcttcttc
     3301 gctagtttta cttggcattt cctatgcaac tcgcttcttc ggtctactca ttgttcgtct
     3361 ggtacaattt gttcttaaaa aaggaaagag aggtaatcag catgcgtaaa tggacaaaag
     3421 gatttctcat ctttggtgtg gtgactaccg ttatcggctt tatcctgctt tttgtaggta
     3481 tccaatctga cgggattaag agcctacttt ccatgtccaa agaacctgtc tatgatagcc
     3541 gtacggaaaa gctaaccttt ggcaaggaag tcgaaaacct agaaattact ctccaccaac
     3601 acacgctcac catcacagac tctttcgatg atcaaatcca catttcttac catccatctc
     3661 tttctgctca ccatgatctt atcaccaatc agaacgatag aactctgagt ctcactgata
     3721 agaaactgtc tgaaactccg tttctctctt ctggaattgg tgggattctt catatcgcaa
     3781 gtagctactc tagtcgtttt gaagaagtta ttctccgact accaaaaggg agaactctaa
     3841 aagggatcaa catctcagcc aatcgcggac aaaccaccat cataaatgct agccttgaaa
     3901 atgcgaccct caatacaaac agctatatcc tccgaattga aggaagtcgt atcaaaaaca
     3961 gtaaactcac aacgcccaat atcgttaata tctttgatac agttcttaca gatagtcagc
     4021 tagagtcaac agagaatcac ttccacgctg aaaatatcca agtccatggc aaggttgaac
     4081 tgactgccaa agattatctc agaatcatcc tagaccagaa agaaagccaa cgaattaact
     4141 gggacatctc aagcaactat ggttctatct tccaattcac aagagaaaag cctgaatcaa
     4201 gaggtacgga attaagcaac ccttacaaaa ctgaaaaaac cgatgtcaag gatcaactca
     4261 ttgcgagatc tgatgataat attgatctaa tatccacacc aagcagacgt tgacaaaaac
     4321 gcttacattt gctactatgg aaacatattt atctaactaa aaaggaggtt ctaccatgaa
     4381 acaagaatgg tttgaaagta atgattttgt aaaaacaaca agcaagaaca agcctgaaga
     4441 gcaagctcaa gaggttgcag acaaggctga agaaacgata gccgatctcg atacaccaat
     4501 tgaaaaaaat actcagttag aggaggaagt ccctcaagct gaagtcgaat tggaaagcca
     4561 gcaagaagag aaaattgaag ctcctgaaga cagtgaagcg agaacagaaa tagaagaaaa
     4621 gaaggcatct aattctactg aagaagagcc agacctttct aaagaaacag aaaaagtcac
     4681 tatagctgaa gagagccaag aagctcttcc tcagcaaaaa gcaaccacga aagagccact
     4741 tcttatcagt aaatctttag aaagtcctta tatccccgac caagctccaa aatctaggga
     4801 taaatggaaa gagcaagtgc ttgatttttg gtcttggcta gtggaagcga tcaaatctcc
     4861 tacaagtaag ttggaaacaa gtatcacaca cagttacaca gcctttctct tgctcattct
     4921 gttttctgca tcttcctttt tctttagtat ctatcacatc aaacatgctt actatggaca
     4981 tatagcaagc attaacagtc gcttccctga gcagctagct cctttaactc ttttttctat
     5041 catctctatc ctagtagcga caacactctt cttcttttca ttcctcttgg gtagtttcgt
     5101 tgtgagacga tttatccacc aggaaaagga ctggacgcta gacaaggttc tccaacaata
     5161 tagtcaactc ttggcaattc caatcttcct cactgctatt gctagtttct ttgctttctt
     5221 tgatagccta cgatttacag ccctcttgtg tgtgattagc attggaatca ttctgcttgc
     5281 cagtctccat atcattacaa gaccaagtca agcaagtgaa accgactcct tctatcaatt
     5341 attcttgtct gtccttgtga acggagttat tatcctcctc ttctttgtag ctgaagtggc
     5401 actgattgga gattatcttc gtatcttggc ctttctttaa acttcatact cttcaaaaat
     5461 caaattcaaa ccacgtcagc ttcgccttac cgtactcaag tacagcctgc ggctagcttc
     5521 ctagtttgct ctttgatttt cattgagtag caatcctgtc atccttggca ggattttttc
     5581 tatgtcaaaa aagcaagtcg attgacctgc ttctgcttta cttttcttgt gctaatgttt
     5641 taaaatcttt gtctaatatg ggttgcactt ctatttgatt ggcgtctagt tggtggtaaa
     5701 aagatggggg taatacctct aagaattttg catagtccaa gcgagcaatg gcttcatagt
     5761 cttctggttt agagccatct cctgtgattt gaaccaagtc aatctcccac tggacttcct
     5821 tatccaccaa ttgctcaata taggctgcag gaacaaacgg ttctctcggt aaaactgtct
     5881 tgactccttg agctagatgg taaacgccat gcacttttcc ttctccatcc tgatagaagg
     5941 aaactctctc aaagtaggca tctgtctctt gaaccgcacg cacacgcgct tcaaactgag
     6001 ccaagccatc tgcctctaaa aagcttttca agttctcacg actaaagaaa ataggattaa
     6061 aaattccttg gcaaatcgta aaacgagcta cagggtctgc tagataggct tgaatacttg
     6121 cttggaaata agcttcaaaa tcaaaaccat ctagcccttc aatatcagtc cctgtataag
     6181 caagcaaagc atctaaaaat gacttgataa tctgccagtc tgtctttgtt agtaggtcag
     6241 gaagatcgac acggtaagcc ttttgaccat cagcataact gaccttaaag agaaattgtg
     6301 attgccccac tatcgcacac tcgatatatt cgagacgatt aagaggctga cggagataga
     6361 ctacatcata gctatgtgac tccaaaccgt ctagcaaggc caagatcgac ttggcattca
     6421 aaatctcttg ttctcctaaa atgctctgtt tatttggaat aaaaaatgtt ttcgccataa
     6481 ctggactcct ttgaaatcgt ttacatacag tataccttat ttttctgaaa gatacaaaaa
     6541 caaactttat actcttcgaa aatctcttca aaccgcgtca gcttcgcctt gccgtagata
     6601 tatgtaactg acttcgtcag tcttatctac aacctcaaag cagtgctttg agcagcctgc
     6661 agctagcttc ctagtttgct ctttgatttt cattgagtat tagatttttg agtacaaagc
     6721 atagaaaaac agcccctaag gactgtttga tcttatacag tagctgcctg atccaagctt
     6781 tcaccgatag cggctaggcg ctcaatcact tcagcttgtg tcaattcatt ttttgaaaca
     6841 tagcggttac gtgggtgaac acggcactcg tgtgagcatc cacgaaggta cttgtcttca
     6901 ttttcttctg atgtcaagat acgacggtta cagaagggat ttccacagtt gacataacgt
     6961 tcacatggtg ttccatcaaa ccagtctttc cctacgatag ttgggttgac atggttgaca
     7021 tcaacggcaa tacgctcgtc aaagacgtac attttcccat cccaaagctc accttgaact
     7081 tctgggtctt taccgtaagt tgcgattcct ccgtgcaatt ggccgacatc tttgtagcct
     7141 tcacggacca tccagcctga gaatttctca cagcgaacgc cacctgtaca gtaaaccacg
     7201 acacgcttgt ccatgaattt ttccttgtta tcacggaccc attgtggtaa ctcacggaag
     7261 ttgcgaatat ctgggcgaat agccccacgg aaatgtccta ggtcgtactc ataatcgtta
     7321 cgtgtgtcaa ggacaacggt atctttatca agaagcgctt ctttgaactc ttttggagac
     7381 aagtaagcac ctgttgtttc aagtgtgttg atgtcattgt caaagtcgtt gtcttccaaa
     7441 ccaaggtgga caatttcttt cttgtagcga acaaacatct tcttgaaggc ttgttctctt
     7501 tcttcgtcaa tcttgaacca gagatcttcc atacctggga ggctgtgaac gtagtccatg
     7561 tatttttgag ttgtttcata gtcacctgaa actgttccgt taattccctc gtcagcgact
     7621 aggatacggc ctttaaggcc gattgattta cagaaagcca agtggtctgc agcaaattgc
     7681 tctgcatttt caattggagt ataaaggtag taaagtaaga cacgaatatc ttttgccata
     7741 agatttgttc tcttttctat tcttaaattt tctaaatttt ttatttatcc atactagtat
     7801 acctgcttga gaaaacgaat gcaatttatt tgactggaaa ttgtagaggg ttctcgaaat
     7861 ttcctgaatg gttaaaataa ggacaagaga aaacatggat atctatatcc ttgtgccaaa
     7921 aaaaccactg ccctccccag accaacctga ggaaagcagt gattcttatt ttaggagtta
     7981 ggaatgaata cacgaaatca atttagctga ttattttttg tttttcaaga attcatcgta
     8041 ttgtttttgc atttcgttca atactttttc gtaggcacct tcagatttca atttttccat
     8101 caattctgga atagctttat ctgggtctac agtaccagtg ttgatagctg tatcaaattg
     8161 ttgcattgtg ttagcaatag ctgagatttc agatttcaca ttgtcagtat tgaagataaa
     8221 tccaagcgct ggagattctt tagcttctgc caattctttc ttagaatttt cgatttgttg
     8281 gtctgtaacg ttttcgttga tgtaaaggat ccagttgtta ccagtgttcc atccacccat
     8341 gtgagtgttt cctttgtagc catcaagaac gcgaacacgg ttttctttac cttcaatttt
     8401 ttcccagttc ttgccttctg gaccgtaaac aagaccgttc aagagttctg gattcgtatt
     8461 caagaggttc aagatttcca ttgatttttc tttgttctta gagttgtttg agatgacaaa
     8521 gttagcaact tgtgttgttt ggtttttctt gatgaagtta gtaattggtt tgatttggat
     8581 atctttgttg gcaacacgtg aaagcaagct gttaccgtag tcagctggtc ctactgtttc
     8641 ttcacgaacg aaccaagtat cttgttgaag gtcaaaggaa gtatcgcttg ttgcgacatc
     8701 ttttggaatg tagccagctt catagaattt gtgaagagtc ttcaagtgtt ctttgaaacg
     8761 aggcacttcg taacggttta caactttagt agtatcgcct tcaaggtcaa taacgaatgg
     8821 aagcccgttt gctactgggt agtcaaaatt atcagatggg atgaaaactt taccaatagc
     8881 aaatggtact acgtctggag ctttttcttt gatttgtttc aagactggct caagagtttc
     8941 gtaagaagta acacctgaaa tatcgatacc atatttggca aggagagttc cgttgaaggc
     9001 aaagttttga gatgatgcta cgttggctgc aactggtaca gcataaatct taccatttac
     9061 agtattaccc ttgatataag ctggatcaag tgccttgtaa aggtctttac cttctttttt
     9121 gtacaattct gtcaagtcag cataagcacc tttttgagca tttacaatat agttatctgc
     9181 aaaggcgata tcatagtttt caccagatga tgtgataact gacattttct taccatagtc
     9241 accccagcca aggtattgga tatccaattt ggcaccaact ttttcttcga tgattttgtt
     9301 ggcatttgct aacaattcat ccaagttatc tggtttgtca ccgatttggt acattttgat
     9361 aacaggtttg tcacctgaat cagcagcttt tttactgtta cctgtcaagt tgccacaagc
     9421 agcaagacct gcagccaaag cgactacact agcagatgca aaagcatatt ttttccagtt
     9481 tttcatgata aaaactcctt tttttatttt taaacttata aacaatgtaa tgatcttata
     9541 ctcaatgaaa atcaaagagc aaactagaaa actagccgca ggctgtactt gagtacggca
     9601 aggcgacgtt gacgcggttt gaatttgatt ttcgaagagt attaacttca cacaagggaa
     9661 gttgggaact gagaaatgtt atttctcaat aagcactatt ctttcacacc accgatagtc
     9721 aaacctttta caaagtagcg ttggaaaaat ggatacaaaa tcgcgattgg aagggttgca
     9781 accacaacca tggccatacg acctgtttct ttcggtagag caactcccag ttgaccagtc
     9841 aagccgaccg ctttggcaat gtagtccata ttttgttgga tttgcatgag caaatattgc
     9901 aatggataca agttgtcact cttgatgtaa agaagggcgt tgaaccagtc attccagaaa
     9961 ccaagagctg ttaagagcgt gatggttgcg atacctggta gtgacaatgg caaacagatt
    10021 tggaagaaaa tccgggcttc actggcacca tcgatacgag ccgattctag aatggcttct
    10081 ggaatggtct tcttgaagaa ggaacgcatc aagatgatgt taaatggtga gagaagcatt
    10141 ggaacaatca aggcccaaac agtatcccca agctgaagca cacgagtcac catgatataa
    10201 cctggtacca aaccagcgtt gaacaacata ctgagcagaa cgaagatggt aaagaatctg
    10261 cgatacttaa aggttgtccg tgaaatagcg taggcatagg ttgttgtgat aaagacattt
    10321 gtcaatgtcc caactacggt tacaaagaca gagatgaaga gggcttgtag gattttatcc
    10381 ttaaactgtg ccaaaaactc aaaaccgtct aagccaaatt gggatgggaa gaagctatag
    10441 ccgtattgga ggaggctttt ctcgtctgtc actgaaataa tgataacgaa tacaaaaggt
    10501 aggatacaag agagggcaat caaacccgaa atgatactga agaagatatc tgctttctta
    10561 ctgaaggagt gaatgccgac attatcaatt ttttcttttt taattttctt ttttgccata
    10621 ttctcctcct ttctagaaca aagctgagtt tggatcgact cgtcttgcaa gcaagtttga
    10681 taggataacc agaatcaaac caacaacgga ttggtaaaga ccggctgctg cagccatacc
    10741 gatatctgct gtctgagtca aaccattaaa gacatatacg tccaaaacgt tggttacatt
    10801 gtaaagctga ccagcattgt gtgggatttg atagaagaga ccaaagtctg cgcggaagat
    10861 atttccgact gcaaggatgg tcaatacagt tacaagcgga gtcaactgag gaatggttac
    10921 gttgcgaata cgttgccact tgctagctcc gtccactgtc gctgcttcgt agtaggttgg
    10981 atcaattccc atgatcgtcg catagtacat gacactgcta tatccaaagc ctttccaaat
    11041 acctaggaaa agtaggagat agggccagat gcccaggtca gcgtaaaaat tgacttcttt
    11101 gagaccaaga ctttccaata gatgattgaa caccccttta tcaatattta ggaaggcatc
    11161 tgtaaagaaa ctgatgataa cccaagacaa gaagtaaggg aacaacatag aagtttggaa
    11221 aatcttcacc attctcttag aacggagctc gctgaggata atggcaatcc ctacagatac
    11281 aactaaacct agaaagataa agccaagatt gtagaggaca gtatttcgtg tgataataaa
    11341 ggcgtctctt gaactaaata agaatctgaa attatcgagt ccgacccatt tactatttat
    11401 gatactatct atgaaaccat tactggtcat gtggtagtct ttgaaggcaa ccacgttccc
    11461 aaatactgga atgtaaaaga atagaatcaa ccagagtgtc cctggcaaaa ccatcaagag
    11521 aaagatccag ttgtctctca atgtttttga aaactttttc ataatttcct ccctttttat
    11581 tttgatatcc atctaaaaat tcttttttag acttttgata acgattacat tattagtata
    11641 ctcctatttg caggttaggt taaactccta attatagaaa aaactccaca aattatgtag
    11701 cagatttaaa actttatcac cactatcaaa caaatgtcct aaatcaattg tttattttat
    11761 ctctattagc ccagtgatgg cgtcactctg ttataagcat ccaacaacgg ggtatactga
    11821 aaaatctcca gactagggaa ctcagagata gttcctaatc tggagatttt taatatgtta
    11881 ttaggcgttt gctttcaact tagcaataac ctctttaaga ttatcaatca cctctgcttc
    11941 agtatgctca gagctttttc atctgccaag aacaaaactg ctttttgaag ttctttttga
    12001 gagttttcaa ggacatcctt atctactgtt tcaaggtttg agtctttaag aagtttactt
    12061 aattccttgg ctaatttctt gagtttgatt tgcagactca tcttctcctg ctgtttcttt
    12121 gcccgctgtt tgtcctccat ccttagttgc tgactggctt tccttaatgg actctaggga
    12181 agcaatggca tctttgactg tttgcaaaat atcacgtaaa ccttgctctg tcaaactatc
    12241 atctgcaaaa gctttcttag cctctgccaa aaccagacgt gctgaatctg tggtaggatt
    12301 cgatacacct gtcaatgatc tcaaaagatt ttctaaggtt tgagtctgct tactaatact
    12361 agactaaaat caaaaagcat tatataacag tgatatgaaa tcaactaaag aagaaatcca
    12421 aac
//
LOCUS       DAVLYR010000046        12249 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLYR010000046 DAVLYR010000000
VERSION     DAVLYR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.046"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            226..897
                     /locus_tag="VYO63_000992"
     CDS             226..897
                     /locus_tag="VYO63_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1671935.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(949..1146)
                     /locus_tag="VYO63_000993"
                     /pseudo
     CDS             complement(949..1146)
                     /locus_tag="VYO63_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1139..2047
                     /locus_tag="VYO63_000994"
     CDS             1139..2047
                     /locus_tag="VYO63_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1671936.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2044..2505
                     /locus_tag="VYO63_000995"
     CDS             2044..2505
                     /locus_tag="VYO63_000995"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1671937.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2495..3382
                     /locus_tag="VYO63_000996"
     CDS             2495..3382
                     /locus_tag="VYO63_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1671938.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3385..5268
                     /gene="cbpE"
                     /locus_tag="VYO63_000997"
                     /gene_synonym="pce"
     CDS             3385..5268
                     /gene="cbpE"
                     /locus_tag="VYO63_000997"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1671939.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5348..6478
                     /locus_tag="VYO63_000998"
     CDS             5348..6478
                     /locus_tag="VYO63_000998"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1671940.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6488..7750
                     /locus_tag="VYO63_000999"
     CDS             6488..7750
                     /locus_tag="VYO63_000999"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1671941.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAIGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7754..8551
                     /gene="proC"
                     /locus_tag="VYO63_001000"
     CDS             7754..8551
                     /gene="proC"
                     /locus_tag="VYO63_001000"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1671942.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            8771..9409
                     /locus_tag="VYO63_001001"
     CDS             8771..9409
                     /locus_tag="VYO63_001001"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1671943.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            9406..10296
                     /locus_tag="VYO63_001002"
     CDS             9406..10296
                     /locus_tag="VYO63_001002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1671944.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10336..10653
                     /gene="yabA"
                     /locus_tag="VYO63_001003"
     CDS             10336..10653
                     /gene="yabA"
                     /locus_tag="VYO63_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1671945.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10656..11525
                     /gene="rsmI"
                     /locus_tag="VYO63_001004"
     CDS             10656..11525
                     /gene="rsmI"
                     /locus_tag="VYO63_001004"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1671946.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11752..>12249
                     /locus_tag="VYO63_001005"
     CDS             11752..>12249
                     /locus_tag="VYO63_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1671947.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRK"
BASE COUNT         3669 a         2121 c         2915 g         3544 t
ORIGIN      
        1 gatacaaggt ggagcagatt catatggtaa aaatcctaat ggtaggtacg attgggaaat
       61 agaaccagta ttaactctgc cggttcatgg attttgtccc agaggcacct atgatttagg
      121 atatattgga ggaggcaatc atctttgcaa aggaagtgct gcgagatttt aagtaaaatt
      181 tattagtaat atgaagaaac aaggggagaa agcagagaat ttaatatgaa aaaacgggct
      241 attcaaattt tactagcatt gtccttaatt ttttacaaat caacttggtt ttggagagtt
      301 ttcaatcacc tcgcaaagcc ctatctacca gcaagtcgtg aattttttca gattctgctt
      361 ttgatggaga gcggagttct tttcttagcg gtcatctatc tactggtttt tgcaggaaag
      421 aaaatttttc atttcaagtg gcagctgagg tacttcatct accttttact gggctacatc
      481 atttcatata tgtctgactt cctcttttcg tatttcatat ccctgtcttc aaatcagatt
      541 tctttgaatg aaacgataga aatgatgggg agacaggagt tcccttattt cttgctcatc
      601 gtttgcttca tcgcccctat tgctgaggaa ttgatttatc gaggtgtgct tatgacaacc
      661 tttttcaaaa actcaccttg gtacggagat gttttgcttt ctgctattat tttcggttat
      721 attcatatca attttgcttt aactcctctt gcttttttca tttatgctag tggaggtctg
      781 attttagctc tattgtatcg catgactaaa aatctctact atccaatact agttcatatt
      841 ctcatcaata tcactgcctt ctgggatgtt tggttactcc tattttcagg aagttagctt
      901 actaaaaaaa tgtcggaatt ttccggcatt ttcttttttc acaaatagtc aacgtttttc
      961 tttccgatac tgaagtggtg tgtagccact tatttttttg aattgatttt gaaaataaga
     1021 ttggcgtgag aaaggcagat agtgaagata gttaagaaga ataggatgtt cttttttcct
     1081 ttttggaaaa cttctaaaat atggtataat gaaaagataa agaagttagg ggtagaagat
     1141 gaacattcaa caattacgct atgttgtggc tattgccaat agtggtactt ttcgtgaagc
     1201 tgctgaaaag atgtatgtta gtcagccgag tctgtctatt tctgttcggg atttggaaaa
     1261 agagttgggt tttaagattt tccgtcggac cagctcaggg actttcttga cccgtcgtgg
     1321 gatggaattt tatgaaaaag cgcaagaatt ggttaaagga tttgatattt ttcaaaatca
     1381 gtatgccaat cctgaagaag aaaaagatga attttccgtt gctagccagc actatgactt
     1441 cttaccacca actattacgg ccttttcaga gcgctatcct gactataaga acttccgtat
     1501 ttttgaatca actactgttc aaatattaga tgaagtggcg caagggcata gtgagattgg
     1561 gattatctac ctcaacaatc aaaataaaaa ggggattatg caacgggttg aaaagttagg
     1621 tctggaggtc atcgaattga ttcctttcca tacccatatt tatctctgtg agggtcatcc
     1681 tttagcccag aaagaggaat tagtcatgga ggatttagcg gatttaccaa cggttcgttt
     1741 cactcaagag aaagacgagt acctttatta ttcagagaac tttgttgata ccagcgctag
     1801 ctcacagatg tttaatgtga cagaccgtgc caccttgaat ggtattttgg agcggacgga
     1861 cgcctatgcg acaggttctg gatttttaga tagtgacagt gttaatggca ttacagttat
     1921 tcgtctcaag gataacctag ataaccgcat ggtctatgtt aaacgtgaag aagtggagct
     1981 tagtcaagct gggactctct tcgtagaagt catgcaagaa tattttgatc aaaagaggaa
     2041 atcatgaaaa aaagagcaat agtggcagtc attgtactgc ttttgattgg gctggatcag
     2101 ttggtcaaat cctatatcgt ccagcagatt ccactgggtg aagtgcgctc ctggatcccc
     2161 aatttcgtta gcttgaccta cctgcaaaat cgaggtgcag ccttttctat cttacaagat
     2221 cagcagttgt tattcgctgt cattactctg gttgtcgtga taggtgccat ttggtattta
     2281 cataaacaca tggaggactc attctggatg gtcttgggtt tgactctaat aatcgcgggt
     2341 ggtcttggaa actttattga cagggtcagt cagggctttg ttgtggatat gttccacctt
     2401 gactttatca actttgcaat tttcaatgtg gcagatagct atctgacggt tggagtgatt
     2461 attttattga ttgcaatgct aaaagaggaa ataaatggaa attaaaattg aaactggtgg
     2521 tctgcgtttg gataaggctt tgtcagattt gtcagaatta tcacgtagtc tcgcgaatga
     2581 acaaattaaa tcaggccagg tcttggtcaa tggtcaagtc aagaaagcta aatacacagt
     2641 ccaagagggt gatgtcgtca cttaccatgt gccagaacca gaggtattag agtatgtggc
     2701 tgaggatctt ccgctagaaa tagtctacca agatgaggat gtggctgtcg ttaacaaacc
     2761 tcagggaatg gttgtgcacc cgagtgctgg tcataccagt ggaaccctag taaatgccct
     2821 catgtatcat attaaggact tgtcgggtat caatggggtt ctgcgtccag ggattgttca
     2881 ccgtattgat aaggatacgt caggtcttct catgattgct aaaaacgatg atgcgcatct
     2941 agcacttgcc caagaactca aggataaaaa gtctctccgc aaatattggg cgattgttca
     3001 tggaaatcta cctaatgatc gtggtgtaat tgaagcgccg attggccgga gtgaaaaaga
     3061 ccgtaagaaa caggctgtaa ctgctaaagg gaagcctgca gtgacgcgtt ttcacgtctt
     3121 ggaacgcttt ggcgattata gcttagtaga gttgcaactg gagacagggc gcactcatca
     3181 aatccgtgtc cacatggctt atatcggcca tccagtcgct ggtgatgagg tctatggtcc
     3241 tcgcaagact ttgaaaggac atggacaatt tcttcatgcc aagactttag gttttactca
     3301 tccgagaaca ggtaagacct tggaatttaa agcagatatc ccagagattt ttaaggaaac
     3361 cttggagaga ttgagaaagt aagaatgaaa aagaaattaa ctagtttagc acttgtaggc
     3421 gcttttttag gtttgtcatg gtatgggaat gttcaggctc aagaaagttc aggaaataaa
     3481 atccacttta tcaatgttca agaaggtggc agtgatgcga ttattcttga aagcaatgga
     3541 cattttgcca tggtggatac aggagaagat tatgatttcc cagatggaag tgattctcgc
     3601 tatccatgga gagaaggaat tgaaacgtct tataagcatg ttctaacaga ccgtgtcttt
     3661 cgtcgtttga aggaattggg tgtccaaaaa cttgatttta ttttggtgac ccatacccac
     3721 agtgatcata ttggaaatgt tgatgaatta ctgtctacct atccagttga ccgagtctat
     3781 cttaagaaat atagtgatag tcgtattact aattctgaac gtctatggga taatctgtat
     3841 ggctatgata aggttttaca gactgctgca gaaaaaggtg tttcagttat tcaaaatatc
     3901 acacaagggg atgctcattt tcagtttggg gacatggata ttcagctcta taattatgaa
     3961 aatgaaactg attcatcggg tgaattaaag aaaatttggg atgacaattc caattccttg
     4021 attagcgtgg tgaaagtcaa tggcaagaaa atttaccttg ggggcgattt agataatgtt
     4081 catggagcag aagacaagta tggtcctctc attggaaaag ttgatttgat gaagtttaat
     4141 catcaccatg ataccaacaa atcaaatacc aaggatttca ttaaaaattt gagtccgagt
     4201 ttgattgttc aaacttcgga tagtctacct tggaaaaatg gtgttgatag tgagtatgtt
     4261 aattggctca aagaacgagg aattgagaga atcaacgcag ccagcaaaga ctatgatgca
     4321 acagtttttg atattcgaaa agacggtttt gtcaatattt caacatccta caagccgatt
     4381 ccaagttttc aagctggttg gcataagagt gcatatggga actggtggta tcaagcgcct
     4441 gattctacag gagagtatgc tgtcggttgg aatgaaatcg aaggtgaatg gtattacttt
     4501 aaccaaacgg gtatcttgtt acagaatcaa tggaaaaaat ggaacaatca ttggttctat
     4561 ttgacagact ctggtgcttc tgctaaaaat tggaagaaaa tcgctggaat ctggtattat
     4621 tttaacaaag aaaaccagat ggaaattggt tggattcaag ataaagagca gtggtattat
     4681 ttggatgttg atggttctat gaagacagga tggcttcaat atatggggca atggtattac
     4741 tttgctccat caggggaaat gaaaatgggc tgggtaaaag ataaagaaac ctggtactat
     4801 atggattcta ctggtgtcat gaagacaggt gagatagaag ttgctggtca acattattat
     4861 ctggaagatt caggagctat gaagcaaggc tggcataaaa aggcaaatga ttggtatttc
     4921 tacaagacag acggttcacg agctgtgggt tggatcaagg acaaggataa atggtacttc
     4981 ttgaaagaaa atggtcaatt acttgtgaac ggtaagacac cagaaggtta tactgtggat
     5041 tcaagtggtg cctggttagt ggatgtttcg atcgagaaat ctgctacaat taaaactaca
     5101 agtcattcag aaataaaaga atccaaagaa gtagtgaaaa aggatcttga aaataaagaa
     5161 acgagtcaac atgaaagtgt tacaaatttt tcaactagtc aagatttgac atcctcaact
     5221 tcacaaagct ctgaaacgag tgtaaacaaa tcggaatcag aacagtagta gaaaagaagg
     5281 ttttagggcc ttctttttcc tatcaactct tttctatttc ctgttattca tgttataatg
     5341 gataaatatg aataatcgga gtgagactat gaaatacaaa cggattgtct ttaaggtggg
     5401 tacttcttct ctgacaaatg aggatggaag tttatcacgt agtaaggtaa aggatattac
     5461 ccagcagttg gctatgctgc acgaggctgg tcatgagttg attttggtgt cttcaggtgc
     5521 cattgcggct ggttttggag ccttaggatt taaaaagcgt ccgactaaaa ttgctgataa
     5581 acaggcttca gcagcggtag ggcagggact tttgttggaa gaatatacga ccaatcttct
     5641 cttgcgtcaa atcgtttctg cacaaatctt gctgacccaa gatgactttg tggataagcg
     5701 tcgttataaa aatgcccatc aggctttgtc ggttttgctc aaccgtgggg caattcctat
     5761 catcaatgag aatgatagtg tcgttattga tgagctcaag gttggggaca atgacactct
     5821 aagtgctcaa gtagcggcga tggtccaagc agacctttta gttttcttga cagatgtgga
     5881 cggtctctat actggaaatc ctaattcaga tccaagagcc aaacgcttgg agagaatcga
     5941 gaccatcaat cgtgagatta ttgatatggc tggtggagct ggttcgtcaa acggaactgg
     6001 gggtatgtta accaaaatca aggctgcaac tatcgcgacg gaatcaggag ttcctgttta
     6061 tatctgctca tccttgaaat cagattccat gattgaggcg gcagaggaga ccgaggatgg
     6121 ttcttacttt gttgctcaag agaaggggct tcgtacccag aaacaatggc ttgccttcta
     6181 tgctcagagt caaggttcta tttgggttga taaaggggct gcggaagctc tctctcaaca
     6241 tggaaagagt cttctcttat ctggtatcgt tgaagcagaa ggagcctttt cttacggtga
     6301 tatcgtgaca gtatttgaca aggaaagtgg aaaatcactt ggaaaaggac gcgtgcaatt
     6361 tggagcatct gctttggagg atatgttgcg ttctcaaaaa gccaagggtg tcttgattta
     6421 ccgtgacgac tggatttcca ttactcctga aatccaacta ctttttacag aattttagag
     6481 gtaaactatg gtgagtagac aagaacaatt tgaacaggta caggctgtta aaaaatcgat
     6541 taacacagct agtgaagaag tgaaaaacca agccttgcta gccatggctg atcacttagt
     6601 ggctgctact gaggaaattt tagcggctaa tgccctcgat atggcagcgg ctaaggggaa
     6661 aatctcagat gtgatgttgg atcgtcttta tttggatgca gatcgtatag aagcgatggc
     6721 aagaggaatt cgtgaagtgg ttgccttacc agatccaatc ggtgaagttt tagaaacaag
     6781 tcagcttgaa aatggtttgg ttatcacaaa aaaacgtgta gctataggtg tcatcggtat
     6841 tatctatgaa agccgtccaa atgtgacgtc tgatgcggct gctttgactc ttaagagtgg
     6901 aaatgcggtt gttcttcgta gtggtaagga tgcctatcaa acaacccatg ccattgtcac
     6961 agccttgaag aagggcttgg agacgactac tattcatcca aatgtgattc aactggtgga
     7021 ggatactagc cgtgaaagta gttatgctat gatgaaggcc aagggctatc tagaccttct
     7081 cattcctcgt ggaggagctg gcttgatcaa tgcagtggtt gagaatgcga ttgtacctgt
     7141 tatcgagaca gggactggga ttgtccatgt ctatgtggat aaggatgcag acgaagacaa
     7201 ggcgctgtct atcatcaaca atgctaaaac cagtcgtcct tctgtttgta atgccatgga
     7261 ggttctgctg gttcatgaaa acaaggcagc aagcatcctt cctcgcttgg atcaaatgct
     7321 ggttgcagag cgtaaggaag ctggactgga accaattcaa ttccgcttgg atagcaaagc
     7381 aagccagttt gtttcaggtc aagcagctga gacccaagac tttgacaccg agtttttaga
     7441 ctatgtcctt gctgttaagg ttgtgagcag tttagaagaa gcggttgcgc acattgaatc
     7501 ccacagcacc catcattcgg atgctattgt gacggaaaat gctgaagctg cagcatactt
     7561 tacagatcaa gtggactctg cagcggtgta tgttaatgcc tcaactcgtt tcacagatgg
     7621 aggacaattt ggtcttggtt gtgaaatggg gatttctact cagaaattgc acgcgcgtgg
     7681 tcccatgggc ttgaaagagt tgaccagcta caagtatgtg gttgccggtg atgggcagat
     7741 aagggagtaa gagatgaaga ttggatttat cggtttgggg aatatgggtg ctagcttggc
     7801 aaaatctgtc ttgcagacta ggacgtcaga tgagattctc cttgccaatc gtagtcaagc
     7861 taaggtagat gctttcattg cagactttgg tggtcaggct tccagcaatg aagaaatgtt
     7921 tgcagaagca gatgtgattt ttctaggagt taagcctgct cagttttctg aactgctttc
     7981 tcaataccag accatccttg aaaaaagaga aagtcttctt ttgatttcga tggcagctgg
     8041 attgacctta gaaaaactag caagtcttat cccaagtcaa caccgaatta ttcgtatgat
     8101 gcctaatacc cctgcttcta tcgggcaagg agtgattagt tatgccttgt ctcctaattg
     8161 cagggctgag gacagtgagc tcttttatca gcttttagcc aaggctggtc tcttggttga
     8221 actaggagaa agtttaatcg atgcagcgac aggtcttgca ggttgtggac cagcctttgt
     8281 ctatcttttt atcgaggcct tggcagatgc aggtgttcag acaggattac cacgagaaat
     8341 agcattgaaa atggcagcac aaactgtggt aggagctggg caattggtcc ttgaaagtca
     8401 gcaacatcct ggagtattga aagaccaagt ctgtagccca ggcggttcga ctatcgctgg
     8461 tgtagcaagc ctagaagcgc atgctttccg aggaacagtc atggatgcag ttcatcaagc
     8521 ctacaaacga acacaagaac taggtaaata agaggtagtt ttatctgcct cttttatggt
     8581 ggttgatact cttcgaaaat caaattcaaa ctgcgtcagc ttcaccttgc cgtactcaag
     8641 tacagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat gaaatgagaa
     8701 gacaaaaaga ttgtcacaaa cccctatttt tttgatagaa tagaagtagt aaaaaagaaa
     8761 tgagttagac atgtcaaaag gatttttagt ctctcttgag ggaccagagg gagcaggcaa
     8821 gaccagtgtt ttagaggctc tgctaccaat tttagaggaa aaaggagtaa aggtgctgac
     8881 gacccgtgaa cctggcggag tcttgattgg ggagaagatt cgggaagtga ttttggatcc
     8941 aagtcatact cagatggatg ctaaaacaga gctacttctc tatattgcca gtcgcagaca
     9001 gcatttggtg gaaaaagttc ttccagccct tgaagctggc aagttggtca tcatggatcg
     9061 ttttatcgat agttctgttg cctatcaggg atttggtcgt ggcttagata ttgaagccat
     9121 tgactggctc aatcagtttg cgacagatgg cctcaaaccc gatttgacac tctattttga
     9181 catcgaggtg gaagaagggc tggctcgcat tgctgcgaat agtgaccgcg aggtcaatcg
     9241 tctggacttg gaaggtttgg aattgcacaa gaaagtccgt caaggctacc tttctctcct
     9301 ggaaaaagaa ggaaatcgca ttgtcaagat tgatgctagt ctccctttgg agcaagttgt
     9361 ggaaactacc aaggctgtct tgtttgacgg aatggacttg gccaaatgaa acaagatcaa
     9421 ctaaaggctt ggcaaccagc tcagtttgac cgttttgtcc gtatcttaga acaagaccag
     9481 ctcaatcacg cctatctctt ttcaggtttc tttggaagct tggaaatggc gcaattttta
     9541 gctaagagcc tcttttgtac ggataaagtt ggcgtcttac catgtgagaa atgccgaagt
     9601 tgcaagctga ttgaacagga agagtttcca gatgtcacct tgattaagcc agtcaatcag
     9661 gtcatcaaga cagaacgcat tcgggaattg gtgggacagt tttctcaagc agggattgaa
     9721 agccagcaac aggtctttat tatcgagcaa gcggataaaa tgcatcccaa cgcagccaat
     9781 tctctgctca aggtcatcga agaaccccag agtgaagttt atattttctt cttgactagc
     9841 gatgaggaaa agatgttacc gacaatccga agtcggactc agatcttcca ctttaaaaag
     9901 caagaagaaa aacttatctt actcttagaa caaatgggac ttgttaagaa aaaagcgact
     9961 cttttagcta agtttagtca atcgcgagct gaagcagaaa agttggctaa tcaggcaagt
    10021 ttttggacct tggtcgatga aagtgaacgc ctgctgactt ggttagtagc taagaaaaaa
    10081 gaaagttatc tacaggttgc caaattagcc aacttggcag atgataagga aaaacaggat
    10141 caggttttac ggattcttga agttctctgt gggcaggacc tcttgcaggt aagagtaaga
    10201 gtgattctac aagatttact agaagctaga aaaatgtggc aagctaatgt cagctttcaa
    10261 aatgccatgg aatatctggt cttgaaagaa atataaactc aaaaatgaat gataaagaaa
    10321 ggaaagggct gttttatgga caaaaaagaa ttatttgacg cgctggatga tttttcccaa
    10381 caattattgg taaccttagc cgatgtggaa gccatcaaga aaaatctcaa gagcttggta
    10441 gaagaaaata cagctcttcg cttggaaaat agtaagttgc gagaacgctt gggtgaggtg
    10501 gaagcagacg ctcccgtcaa ggccaagcat gttcgcgaaa gtgtccgtcg tatttaccgt
    10561 gatggatttc acgtatgtaa tgatttttat ggacaacgtc gagagcagga cgaagaatgt
    10621 atgttctgtg atgagttgtt atacagggag taagcatgca aattcaaaaa agttttaagg
    10681 ggcagtctcc ctatggcaag ctgtatctag tggcaacgcc gattggcaat ctagatgata
    10741 tgacttttcg tgctatccag accttgaaag aagtggactg gattgctgct gaggatacgc
    10801 gcaatacagg gcttttgctc aagcattttg acatttccac caagcagatc agttttcatg
    10861 agcacaatgc caaggaaaaa attcctgatt tgattggttt cttgaaagca gggcaaagta
    10921 ttgctcaggt ctctgatgcc ggtttgccta gcatttcaga ccctggtcat gatttagtta
    10981 aggcagctat tgaggaagaa attgcagttg tgacagttcc aggtgcctct gcaggaattt
    11041 ctgccttgat tgccagtggt ttagcgccac agccacatat cttttacggt tttttaccga
    11101 gaaaatcagg tcagcagaag caattttttg gcttgaaaaa agattatcct gaaacacaga
    11161 ttttttatga atcacctcat cgtgtagcag acacgttgga aaatatgtta gaagtctacg
    11221 gtgaccgctc cgttgtcttg gtcagggaat tgaccaaaat ctatgaagaa taccaacgag
    11281 gtactatctc tgagttatta gaaagcattg ctgaaacgcc actcaagggc gaatgtcttc
    11341 tcattgttga gggtgccagt cagggtgtgg aggaaaagga cgaggaagac ttgttcgtag
    11401 aaattcaaac ccgcatccag caaggtgtga agaaaaacca agctatcaag gaagtcgcta
    11461 agatttacca gtggaataaa agtcagctct acgctgccta ccacgactgg gaagaaaaac
    11521 aataaaggga gacaggatgt aataattctg tctgtttctg tttaacttaa ttagtgatga
    11581 taatataaag atgtatcact tggtatagaa gctttggtat taagtttttt attaagccca
    11641 tacggaatac cgatggttgg agcagcagtt atagcgttct tagaaggtat aaatagaaaa
    11701 ataaggtcat tttaaatcaa aggattgata aatcagaaag aaggtgattt tttgcgaaca
    11761 tacgaaaata aagaagaact aaaagctgag atagagaaaa catttgagaa atatatttta
    11821 gaatttgata atattccaga aaatttaaaa gataagagag ctgatgaagt tgacagaact
    11881 ccagcagaaa accttgctta tcaggttggt tggaccaact tggttcttaa atgggaagaa
    11941 gatgaaagaa aggggcttca agtaaaaaca ccatcggata aatttaaatg gaatcaactt
    12001 ggtgaattat atcagtggtt cacagatacc tacgctcatt tatctctgca agagttgaaa
    12061 gcaaaattaa atgaaaatat taattctatc tctgcaatga ttgattcgtt gagtgaggaa
    12121 gaattatttg aaccgcatat gagaaagtgg gctgatgaag cgactaaaac agcgacttgg
    12181 gaagtgtata agtttattca tgtaaatacg gttgcacctt ttggaacttt cagaactaaa
    12241 atcagaaaa
//
LOCUS       DAVLYR010000047        12225 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLYR010000047 DAVLYR010000000
VERSION     DAVLYR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.047"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            143..1372
                     /gene="arcA"
                     /locus_tag="VYO63_001006"
     CDS             143..1372
                     /gene="arcA"
                     /locus_tag="VYO63_001006"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1671948.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1430..2446
                     /gene="argF"
                     /locus_tag="VYO63_001007"
     CDS             1430..2446
                     /gene="argF"
                     /locus_tag="VYO63_001007"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1671949.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2611..3558
                     /gene="arcC"
                     /locus_tag="VYO63_001008"
     CDS             2611..3558
                     /gene="arcC"
                     /locus_tag="VYO63_001008"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1671950.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNSGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3769..5280
                     /locus_tag="VYO63_001009"
     CDS             3769..5280
                     /locus_tag="VYO63_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1671951.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5302..6633
                     /locus_tag="VYO63_001010"
     CDS             5302..6633
                     /locus_tag="VYO63_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1671952.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEENLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            6728..6855
                     /locus_tag="VYO63_001011"
                     /pseudo
     CDS             6728..6855
                     /locus_tag="VYO63_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(7020..7310)
                     /locus_tag="VYO63_001012"
     CDS             complement(7020..7310)
                     /locus_tag="VYO63_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671953.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(7298..7549)
                     /locus_tag="VYO63_001013"
     CDS             complement(7298..7549)
                     /locus_tag="VYO63_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1671954.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            7753..7830
                     /locus_tag="VYO63_001014"
                     /pseudo
     CDS             7753..7830
                     /locus_tag="VYO63_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7948..8832)
                     /locus_tag="VYO63_001015"
     CDS             complement(7948..8832)
                     /locus_tag="VYO63_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1671955.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(8977..10128)
                     /locus_tag="VYO63_001016"
     CDS             complement(8977..10128)
                     /locus_tag="VYO63_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1671956.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10274..12040)
                     /locus_tag="VYO63_001017"
     CDS             complement(10274..12040)
                     /locus_tag="VYO63_001017"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1671957.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
BASE COUNT         3594 a         2558 c         2378 g         3695 t
ORIGIN      
        1 cataaaattg tgctaaatat tataatttat attattttcc gagaaattat agccattgac
       61 attacctagg aattgcgtta taacatacat gtaagcggta cccaaaatgg tgccaagtca
      121 aaatttttaa ggaggaaaat acatgtcttc acatccaatt caggtcttct cagaaattgg
      181 gaaactgaaa aaagttatgt tacaccgtcc aggcaaggag ttagaaaact tgttgccgga
      241 ctatcttgaa aggcttcttt ttgatgatat tcctttcttg gaagatgctc aaaaagaaca
      301 tgatgcattt gcccaagctc ttcgcgatga aggaattgag gttctctacc tagaacaact
      361 cgctgctgaa tcattgacct ctccagaaat ccgcgatcaa tttatcgagg aatacttaga
      421 cgaagccaac atccgtgatc gtcaaaccaa ggttgctatt cgtgaattgc ttcacggcat
      481 caaggacaac caagaattgg ttgaaaaaac aatggctggg attcaaaaag ttgaattgcc
      541 agaaattcct gacgaagcta aagatctaac tgacttagtt gaatcagatt atccatttgc
      601 aattgacccg atgccaaacc tctatttcac tcgcgaccca tttgcaacaa ttggaaacgc
      661 cgtatcgctt aaccacatgt ttgcagacac tcgtaaccgt gaaacactct acggtaagta
      721 tatcttcaaa taccacccaa tctatggcgg aaaagtggat ttggtctaca accgtgaaga
      781 agatacgcgt atcgaaggtg gagacgagct agttctttct aaagacgtcc ttgcagtagg
      841 tatctctcaa cgtacagacg cagcttctat cgaaaaactt ttggtcaaca tcttcaagaa
      901 aaatgttggc ttcaagaaag ttttggcctt tgaatttgct aacaaccgta aattcatgca
      961 cttggatact gtcttcacta tggtagacta tgacaagttc actattcacc cagaaatcga
     1021 aggcgacctt cacgtttact cagttactta cgaaaacgaa aaacttaaaa tcgttgaaga
     1081 gaaaggtgac ttagctgaac ttcttgctca aaaccttggt gtagaaaaag ttcatttgat
     1141 tcgttgcggt ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg acggttctaa
     1201 cactttgacc atcgcacctg gtgtggtagt tgtttatgac cgcaataccg tgaccaataa
     1261 gattttggaa gaatacgggc ttcgcttgat taagattcgc ggaagtgaat tggttcgggg
     1321 ccgtggtgga cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt aatcgctgtt
     1381 cggcattcgt caatagaaaa tgtaaaaaat agaaagagga aataataaaa tgacaaattc
     1441 agtattccaa ggacgcagct tcttagcaga aaaagacttt acccgtgcag agttagaata
     1501 ccttattggt ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc aacaccacta
     1561 ccttgctggc aagaatatcg ctctcctatt tgaaaaaaca tctactcgta ctcgtgcagc
     1621 ctttacaact gcggctatcg accttggtgc tcacccagaa tacctcggag caaatgatat
     1681 tcagttgggt aaaaaagaat ctactgaaga tactgctaaa gtattgggac gtatgtttga
     1741 cgggattgaa ttccgcggat tcagccaacg tatggttgaa gaattggcag aattctcagg
     1801 cgttccagta tggaacggtc taactgacga atggcaccca actcaaatgc tcgctgacta
     1861 cttgactgtt caagaaaact tcggtcgctt ggaaggcttg acattggtat actgtggtga
     1921 tggacgtaac aacgttgcca acagcttgct cgtaacaggt gctatccttg gtgtcaatgt
     1981 tcacatcttc tcaccaaaag aactcttccc agaaaaagaa atcgttgaat tggcagaagg
     2041 atttgctaaa gaaagtggcg cacatgttct catcactgaa gatgctgatg aagcagttaa
     2101 agatgcagac gttctttaca cagacgtttg ggtatcaatg ggtgaagaag acaaattcgc
     2161 agaacgtgta gctcttctta aaccttacca agtcaatatg gacttagtta aaaaagcagg
     2221 caatgaaaac ttgatcttcc tacactgctt gccagcattc cacgatactc acactgttta
     2281 tggtaaagac gttgctgaaa aatttggtgt agaagaaatg gaagtaacag acgaagtctt
     2341 ccgcagcaag tacgctcgcc acttcgatca agcagaaaac cgtatgcaca ctatcaaagc
     2401 tgttatggct gcgacacttg gtaaccttta tattcctaaa gtataatttt agataataaa
     2461 ccgtctacca acagctatga gggctgcgac taatagcttt agtccggtcc tcttttatgt
     2521 aatggtaatc tattatttct tataaaatat gtgaaaaatc attaaattga aatctaaacg
     2581 cattctattg agtgtgataa aggagaattt atggcaaatc gtaaaattgt agtagctttg
     2641 ggaggaaatg cgattctttc ttctgaccca tcagcaaagg ctcaacaaga agctttagtt
     2701 gaaacagcta agcatcttgt aaaattgatt aaaaatggag atgatctgat tatcactcac
     2761 ggtaatggac ctcaagttgg gaatctcttg ctccaacatt tggcatcaga ctctgaaaag
     2821 aaccctgcct tcccactcga ctcacttgtc gctatgacag aaggtagcat cggtttctgg
     2881 ttgaaaaatg ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa cgttgcctct
     2941 gttgtaacgc aagttgtcgt agataaaaat gatccagctt ttgttaactt gagtaaacca
     3001 atcggtcctt tctattcaga agaagaagca aaagcagaag ccgaaaagag cggagcgact
     3061 ttcaaggaag atgctggccg tggctggcgt aaggtcgttg cctcaccaaa acctgttgac
     3121 atcaaagaaa ttgaaaccat ccgtactctt ttaaatagtg gtcaagtcgt cgtagctgca
     3181 ggtggtggcg gtattcccgt cgtcaaagaa aacaatggac atttgactgg tgtcgaagcg
     3241 gttattgata aagacttcgc ttcccaacgt ttggcagaat tggttgatgc agacctcttc
     3301 atcgttttga caggtgtaga ttatgtattt gttaactaca acaagccaaa ccaggaaaaa
     3361 ttggaacatg tgaatgttgc ccagctggaa gaatatatca aacaagatca gtttgcacca
     3421 ggtagcatgc ttccaaaagt agaagcagct atcgcttttg tcaatggtcg tccagaagga
     3481 aaagcagtta ttacttccct tgaaaatcta ggcgccttga ttgaatctga aagtggaaca
     3541 attattgaaa aaggataagt tgttttacta ataagatgta ttctatttct agtatcttta
     3601 tatcaaatta gaaattattc ttgaaaacat gtacaatatt tcaaaagata ttagtttttt
     3661 agactttaat atggtaaaac aaatataaat agaaagcgtt ttcttaaatg tttatttaag
     3721 aaagtagttg gttttttaca ctttgttaga catcaggagg aaaaacaaat gagtgaaaaa
     3781 gctaaaaaag ggtttaagat gccttcatct tacaccgtat tattgataat cattgctatt
     3841 atggcagtac taacttggtt tatccctgcg ggggccttta tagaaggtat ttacgagact
     3901 cagcctcaaa atccacaagg gatttgggat gtcctcatgg caccaattcg ggctatgcta
     3961 ggtactcatc cagaggaagg ttcgctcatt aaagaaacga gcgcagcgat tgatgtagcc
     4021 ttcttcatcc ttatggttgg tggtttcctt ggcattgtca acaaaactgg tgctcttgac
     4081 gtagggattg cctctatcgt gaagaagtat aagggccgcg aaaaaatgtt aattttggta
     4141 ctaatgcctt tgtttgccct cggtggtaca acttatggta tgggtgaaga aacaatggcc
     4201 ttctatccac tccttgtgcc agttatgatg gccgttggtt ttgatagcct gactggtgtt
     4261 gcaattattt tgctcggttc tcaaatcggc tgtttggcat ctactctgaa tccatttgcg
     4321 acaggtattg cttcagcgac tgcgggagtt ggtacagggg acggtatcgt acttcgtctg
     4381 atcttctggg ttaccttgac tgctcttagt acttggtttg tttaccgtta tgcggataag
     4441 attcaaaaag atccgactaa gtcactggtt tatagtactc gcaaagaaga tttgaaacac
     4501 tttaacgtag aagaatcttc atctgtagaa tctacactta gcagcaaaca aaaatcagtt
     4561 ctcttcttat ttgtgttgac attcatcttg atggtattga gcttcattcc atggacagac
     4621 cttggcgtta ccatttttga tgactttaat gcttggttga ctggtcttcc agttattggt
     4681 aatattgtcg gttcatctac ttctgcacta ggtacttggt acttcccaga aggcgcaatg
     4741 ctctttgcct ttatgggtat cctgattggt gttatttatg gtcttaaaga agataagatt
     4801 atctcttcct tcatgaatgg tgctgctgac ttgctcagtg ttgccttgat cgtagcgatt
     4861 gctcgtggta ttcaagttat catgaacgac ggtatgatta ccgatacaat cctcaactgg
     4921 ggtaaagaag gcttgagcgg tctatcttca caagtcttta tcgttgtaac ttatatcttc
     4981 tatctaccta tgtcattctt gatcccatct tcatctggtc ttgccagcgc aactatgggt
     5041 atcatggctc cacttggaga atttgtaaat gtccgtccta gcttgattat cactgcttat
     5101 caatctgctt caggtgtctt gaacttgatt gcaccaacat ctggtattgt gatgggagct
     5161 cttgcacttg gacgtatcaa cattggtact tggtggaaat tcatgggcaa actcgtagtc
     5221 gctattattg tagtgaccat cgcccttctt ctccttggaa ccttccttcc attcctataa
     5281 aatagtgagt gaggtgattc catgaaaata gatataacaa atcaagttaa agatgaattt
     5341 cttatatcat taaaaacctt gatttcctat ccttcagtac tcaatgaagg agaaaatgga
     5401 acaccttttg gacaagcaat ccaagatgtc ctagaaaaaa ctttagagat ttgtcgagac
     5461 ataggtttca ctacctatct tgaccctaaa ggttattacg gatatgcaga aatcggtcag
     5521 ggagcagagc ttctggccat tctctgtcat ttggatgttg ttccatcagg tgatgaagca
     5581 gattggcaga caccgccatt tgaagcaact atcaaagacg gctgggtatt cggacgtggt
     5641 gtccaagatg ataaaggccc ttcgctcgca gctctctatg cagtaaaaag cttgctggac
     5701 caaggtattc agttcaaaaa gcgcgtacgc tttatctttg gtaccgatga ggaaaacctc
     5761 tggcgctgca tggcacgcta caataccatc gaagaacagg ccagtatggg ctttgcacct
     5821 gactcatctt ttcctctgac ctatgctgaa aaagggcttc tacaggtcaa acttcatggc
     5881 ccgggatcgg atcaactaga gcttgaagta ggaggcgcct ttaacgttgt accagacaag
     5941 gccaactacc aaggtcttct ctatgaacag gtttgtaacg gtctcaaaga agctggttat
     6001 gattaccaaa ccactgaaca aaccgtaacg gttctcggag tgccaaagca tgctaaggat
     6061 gctagtcaag gtatcaatgc tgtcatccga ctagctacca ttcttgctcc tctccaagaa
     6121 caccctgctc tcagttttct tgcaacacaa gcaggtcaag acggcacagg aagacaaatc
     6181 tttggtgata tagcagatga accttctggt cacctatcct ttaatgtcgc aggtctcatg
     6241 atcaatcatg aacgttctga aatccgtatt gacattcgga ctcctgtctt agctgacaag
     6301 gaagaactag tagagttgct tacaagatgt gcacaaaact accaactccg ctacgaagag
     6361 tttgactatc tagcgcctct atacgtcgca aaagacagta aactcgttag cacactgatg
     6421 caaatctacc aagaaaagac tggcgataac agtcctgcta tttcatccgg tggtgccact
     6481 tttgctcgca ccatgccaaa ttgtgtagcc ttcggcgcct tattcccagg agcgaagcag
     6541 acagaacatc aggcaaatga atgtgccgtt ctagaagatt tgtaccgtgc tatggatatt
     6601 tatgccgaag ccgtctatcg acttgcaact taatcaggca actgtttcta caaaaaaaaa
     6661 tcgaccgatt aatgaactgc accccaaaag ttagacagaa taaatctaac ttttggggtg
     6721 ttttattatg aaattgaatt atgaagataa agttcagatc tatgaactaa gaaagcaagg
     6781 acaaagcttc aaacagcttt caaaaagatt tatgtggatg tttctggttt aaagtcatct
     6841 gaatctttga gatgagcttt ataaatcgct tttttcagtg tttgcactgg tgtttcgata
     6901 aactcaaact ttttagccgt ggtattgcct gattttatag tatattgaaa ctagaatagt
     6961 acacctctac ttctaaaaca tttttagaaa tcgatttgtc ctgttcttat ttcattttac
     7021 tatatttgag ccacttcgtc tttaacggct ttattcataa gctcttgtaa tttttcttta
     7081 ctatcaatta cttctgattt tccgttgtaa tttattgtaa taggttttaa cttacctaat
     7141 ttctcgacac gctcattaat ttgatctttt ttgaaggctg catatgtttt tcctaagatt
     7201 ttttcgaaaa tatatttatc agatagcggt ttgccttctt cttcagcttg gtttttgtat
     7261 tgatttgaaa cataaggaac aaatccttca tagtaaccta atgctcccat aagttcaaaa
     7321 gcttgttttc taattcaaac cattgcaact cagatttcag cttttcagat aaatcctgct
     7381 catccaaata atgacttaaa attagtgctg aactcgtttc tgtatcctgt acaggctgat
     7441 cacccatacc atcaaaaaat aaactcgttc ctagcaagac cgaacaagct cctattgcat
     7501 atggcctcaa agaaaaacgc tgctttctct caaattgaaa ttctttcatc ccatctccca
     7561 tcattcatta ttactgtata ttttgtatat cagaaatagt ttgtattcac aaatctttct
     7621 agttattccc ttatcattcc taattaaggg agataacata caataatttt tagttaaatg
     7681 tatatcgatg ttttttgttt ttcttaataa acgcaataca aaaagagcct gttgccaagc
     7741 tctttgtact caatgaaaat caaagagcaa actaggaaac tagccacagg ttgctcaaaa
     7801 caccgttttg aggtagtaga taagactgac gaagtcagct caaaacactg ttttgaggtt
     7861 gcagatagaa ctgacgaagt cagtaacatc tatacggcaa ggcgacgctg acgtggtttg
     7921 aagagatttt cgaagagtat tagtctatta tttcttctca gcgcgaaggg ctgacaagat
     7981 ttgtgttcgg atatcatcca caccatttgg agtatttggt aaaaagatgg tttgatttcc
     8041 tttagaggca aaggtattca aggtatccaa atactggttg gtcaagagga tagacatgat
     8101 ttgttcttct gtcatgccaa cattggcttc cttgagttcg gtgatagact ctgccaatcc
     8161 atccacaatc gccttacgtt gttgggcaat ccccacacca tgaaggcggt ctttttctgc
     8221 ttcggcttca gctgcagtga caattttaat cttgtcagct tccgccaatt cttgtgctgc
     8281 gacccgctta cgttgcgccg cattgatttc attcatggat tgcttaactt ctgcatccgg
     8341 ttcgactttg gtaatcaagg ttttcacgat aatgtagccg taagtggtca tttcttctgc
     8401 tacttggtgt tgaacctcaa gggcaatctc atcttttttc tcaaacaatt catccaaggt
     8461 taattttgga acagaagagc gaagagcatc ttcgatataa gatttaatct gagattctgg
     8521 acgtatgagt ttatagtaag catctgtcac gctctgctcg ttgacacggt actgagtcgc
     8581 tacattcatc ataacgaaca cattgtcctt ggtcttagtc tcaaccacaa tatcactttg
     8641 caacaagcgc aactgaatcc gtgctgcaat cgagtcaatc ccaaaaggca agcgaatatg
     8701 aataccgcta ttagcaacct tttggtattt cccaaagcgt tcaataatcg ccaccgactg
     8761 ctgacgaacc acataaactg tactcagtgt gactatcacc aataggagca cacaaacaat
     8821 cagaaaaatc atgaaaaata ttgccataat ggaacctcca caagtatttt tctagtatta
     8881 tagcacattt aaagaaggct gtgccgtttt tactgcgatt tttcctgaaa tgtcaataat
     8941 tagaggtgaa ttgtcctatt gtcgtccaat ctcttgctaa aataactctt tataaaaggc
     9001 aatcgtttct tctaaggttg gcataaatgg atttcctggt gcgcaggcat caatcaaggc
     9061 attcttagaa aggtattcaa agtcgaaatc tttttcttca ataccaagtt cagtcagttt
     9121 cttaggaata cctactgtct cagaaagctt ctcaatctca acaatcgcat aatcggcaca
     9181 ttcttgatct gatttacctt ctacatgaag tcccaaggct ttggcaacat tgcggaaagc
     9241 ttctggtaca cgtttagcat tttcacgttc tataactggt agcaacatgg cacagcaaac
     9301 accatgtggc aaattatata ctgcaccgag ttggtgagcc attgagtgaa cataaccaag
     9361 accagcatta ttaaagctca taccaccaag gaagatggca ttgaccatac cttcacgcgc
     9421 ttcaatatca tgtccatttt ctacagcacg aggaagatat tccttgataa gctcaatagc
     9481 accaatagac agtttcttgg ttacatcata agcaccaggt gttaccaaag cttcaacagc
     9541 gtgagtcaga gcatccatac cagtagcagc agtcaggcct ttaggtttgg aaagcatgag
     9601 ttccgggtca ttaacagaga taagagcaag gctattcttg tcaaccatta ccatcttaac
     9661 cttgcgttct tcatcagtaa tcacatagtt aatagtgatt tctgcagaag ttcctgccgt
     9721 tgtattgata gccacaactg gcaagccttt tttagcagac ttatgaagcc cttcataatc
     9781 ctgtggtttt ccaccatttg tagccatgat agaaatacaa ctagctgcat cctgtggaga
     9841 acccccgcca agactgatga taaagtcaca gccatgttct tgcaaagcag ctaccccatc
     9901 tgtgacattt ttacaagtag ggtttggctc tacatcgcta aagatgacat attcgattcc
     9961 ttctgtatcc agtggtttta aaaccttagg taaaatgtca ctgccttcga tgtacttatc
    10021 tgtcaccaaa agtgcctttt tatagccaag ttccttgata taaggaccca cttcatttac
    10081 aacaccttta ccaataaggt tgactgccgg aacataaaat gtagccatat aattttcctc
    10141 ctgcgcctcc gcgctatcta tttaacatca ttatattagt ttttgtattc gttttcaatt
    10201 atttgtgaat tgtttaacaa atctttttaa aaggatagga gggacataag ttcacctcct
    10261 atatacacaa tttttattta tgtagcggtc ccaacaactg acatgcacga tagtctgctg
    10321 attctagatc ttctgttcca aataaggacc aatttttagg tctaaagata tcttcctcgg
    10381 gtacattatg catattgaca ggaattctca acatagaagc caaggtaatc aagtctgctc
    10441 caatgtgtcc atatgttatg gctccgtgat tagctcccca attattcatg acgtcataga
    10501 cagacttgaa agcacctttt cctgtcaaac gtggagcaaa ccaagtagtt ggccatcctg
    10561 gatctgtacg attatctaaa gtatggtgaa catcttcagg aagttcaagt gtataacctt
    10621 ctgcaatttg tagcactgga ccaacccctt ttagaagatt gagacgtacc attgttactg
    10681 gcatatcccc cttcgtcaag aaacgagttg agaatcctcc tccacggaag tattcgcggt
    10741 ttgctggtgg gaagtctgta ttttcaagca tagcctgcac ttcactttct tccaactccc
    10801 agaatggttt cataacaggt ttgccatctc gagtagcttg ccctgtacca tccaatgtac
    10861 aagaaccaga gttgattaga tgtaagaagc cagctgcagc acaaccctct aaagtatgtc
    10921 ccgttacacg tttaacagcc tctggactcc aataagtgcg cacatcagca aagatttgtg
    10981 gagtatttgt taatagataa ttaaagagca tagacacacc atttagtgaa tcattctctg
    11041 tcgcaaatac aaatggtttt cgaataccat tccagtcaaa ctgagtattg aggaaagttt
    11101 ccataaagtc cccatttggg aaatggtctg tccactgacg ttgaccttgg aaaccagcta
    11161 ctaaagcatg gtgaccaact gcttcttcct caaaaccaag ttcagcaagt cttgggttac
    11221 caaccattaa gtcacgtcca atcatgaaca tcttaataac aaattcccat tgtctatctt
    11281 tttcttcacg gcttaaaaca aggtcttcac ggttatggtc gaatccttct tttacgtttt
    11341 ctttcaccca tttgagcgca cgttcgaact cttcagggtc gtaaatacca cggtccatac
    11401 ggcgtgtgaa ctccgtcata tctaccgatt catttcgcat tcctaggtat tcttggaaga
    11461 aatccggatt tacaatagaa ccaccaatcc ccatcgaaac actccccatt gataagtaag
    11521 ctgtatctct catcaagcca gttgcaagag ctgcccgcgc ataacgtaaa agtttttctt
    11581 tgacatcttc tggaatagct gtatcattag cttcctgaac atctctacca taaatcccaa
    11641 aggctggaat ccctttttga gcatgtgaag ctagtacagc tgcaagatag acagctcctg
    11701 ggcgttctgt cccattaaat ccccaaatag catgaggaat atctggagac atatccatag
    11761 tttcactacc ataacaccag catggtgtaa ctgtaattgt tgcgcaaaca tttgattttt
    11821 taaacaactc atgggaagct gcagcctctg gaacacgacc aatggtagat ggagaaatca
    11881 cacattccac aggttcccca tctggatatt tcaatgtgct tgaaatcaaa tctgccacac
    11941 ttttagccat gttcattgtt tgtacttcaa gtgattcgcg tacaccttga cgacgaccat
    12001 caatagtcgg acgaatccca atacgtggat gttgaatcat actattttcc tcttattttt
    12061 ctgtttcttt catactaccg atgataattt ttaaaattac ttataagtat aaacctctac
    12121 ctctgccaaa cttaaaggaa ttcctgcgtt tctcaaagaa attctaacct tttttccctt
    12181 cagccctttt aagtctatgg atagattatt gctttctaaa ctgtc
//
LOCUS       DAVLYR010000048        12080 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLYR010000048 DAVLYR010000000
VERSION     DAVLYR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.048"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            136..429
                     /locus_tag="VYO63_001018"
                     /pseudo
     CDS             136..429
                     /locus_tag="VYO63_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            536..830
                     /locus_tag="VYO63_001019"
                     /pseudo
     CDS             536..830
                     /locus_tag="VYO63_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1163..2469
                     /locus_tag="VYO63_001020"
                     /pseudo
     CDS             1163..2469
                     /locus_tag="VYO63_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2509..3519)
                     /gene="tsaD"
                     /locus_tag="VYO63_001021"
     CDS             complement(2509..3519)
                     /gene="tsaD"
                     /locus_tag="VYO63_001021"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1671958.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHELPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(3509..3946)
                     /gene="rimI"
                     /locus_tag="VYO63_001022"
     CDS             complement(3509..3946)
                     /gene="rimI"
                     /locus_tag="VYO63_001022"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1671959.1"
                     /translation="MIEIKRIQQHPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(3943..4626)
                     /gene="tsaB"
                     /locus_tag="VYO63_001023"
     CDS             complement(3943..4626)
                     /gene="tsaB"
                     /locus_tag="VYO63_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1671960.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            4804..4989
                     /gene="cibA"
                     /locus_tag="VYO63_001024"
     CDS             4804..4989
                     /gene="cibA"
                     /locus_tag="VYO63_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1671961.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            4991..5143
                     /gene="cibB"
                     /locus_tag="VYO63_001025"
     CDS             4991..5143
                     /gene="cibB"
                     /locus_tag="VYO63_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1671962.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5466..5681)
                     /locus_tag="VYO63_001026"
     CDS             complement(5466..5681)
                     /locus_tag="VYO63_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671963.1"
                     /translation="MYNLFAFAIGVRLLAFIVFVACVYGGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            5961..6194
                     /locus_tag="VYO63_001027"
     CDS             5961..6194
                     /locus_tag="VYO63_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1671964.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6196..7875
                     /locus_tag="VYO63_001028"
     CDS             6196..7875
                     /locus_tag="VYO63_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1671965.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(7973..8050)
                     /locus_tag="VYO63_001029"
                     /pseudo
     CDS             complement(7973..8050)
                     /locus_tag="VYO63_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8254..10167)
                     /gene="mnmG"
                     /locus_tag="VYO63_001030"
     CDS             complement(8254..10167)
                     /gene="mnmG"
                     /locus_tag="VYO63_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1671966.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVSCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10177..10632)
                     /locus_tag="VYO63_001031"
     CDS             complement(10177..10632)
                     /locus_tag="VYO63_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1671967.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(10773..11894)
                     /gene="mnmA"
                     /locus_tag="VYO63_001032"
     CDS             complement(10773..11894)
                     /gene="mnmA"
                     /locus_tag="VYO63_001032"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1671968.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3399 a         2852 c         2430 g         3399 t
ORIGIN      
        1 ttctcttgtc tcgaccttct tcaaagaatt gctataatac tattacaaat acatctgcac
       61 tacacttcaa attttagcac tgtataaaaa cgtttcaaca cactaacttt aagaaaactt
      121 ccactattaa ttgaaaaagt tgatagagat aaattaaaga tctatattga aactcatccc
      181 gatgcttatt tgactgaaat agctgctgaa ttcaactgcc ctccaacaac tattcattac
      241 gctctaaagg ctatgggata tagtctaaaa aagagccgta cctactgcga acaagaccca
      301 gaaaaagtaa atcggttcct taaagaattg aatcacttaa gctacctgac tcctatttat
      361 atttatgaga caggggttaa gacctatttt tatctcgaat atgatcgagc cttgagcagg
      421 cagttagtct ctctggaaga agatataatt atttgaatta agatcgagac aacgcacacc
      481 agagattgcg atactgttat agaagtacta atgccctttt ttgtttcaat atactatggc
      541 tccgatgacc tataaagata cgatgacgag tgactttttc gaagcttgct tccaaaaatt
      601 cttactacct actttagata caccatccct tatcattatg gacaatgcaa ggtttcacag
      661 aatgaacatg tgtaaggagc agggcataga ctgttaccac ttcctatcta ttcacccgag
      721 tataatccca ttgagaaaat atgggcttac atcaaaaaca tctcagaata atattgtcaa
      781 attacgatgc ttttcttgag gcacttttgt cctattcttg tttcagctga ttatactccg
      841 ttattgggca gctacggaac agtcgatggg acgatggggg gacataaaaa aatcctccag
      901 ttttgttttt tataacagta tactggagaa ttgacaatct cggtagatac ctcgttatag
      961 cgcggttact tattaggcag ttacaaaaca actgtgaaca gaaaacattc cagagtcaga
     1021 caagactttg gaatgctttg gctctataat ttctgtagtg ggtaatccca ccccaggaat
     1081 tatagggtcg tttcttgtag aaaaaaagcc ccatatgacc tataatgaaa agcgtctaac
     1141 caactcatta gaaagggttc atatggaaca acttaagaat accacagatt tgctcggatt
     1201 ggaagacaaa aatatcaaaa tcttgtctgt tctgaaatac caaacccatc tagtcgttca
     1261 ggcaaagttg gattcccccg ctcctccttg tcctcattgt caagggaaga tgatcaaata
     1321 cgacttccag aaagcctcta aaattccgct tctcgactgt cagggtttac ccacggtact
     1381 gcatctcaaa aagcgccgct ttcagtgcaa gaattgcctt aaggtggtcg tttctcaaac
     1441 atccattgtc aagaaaaatt gccagatttc caacatggtg agacaaaaaa tcgctcagct
     1501 cctccttgaa aagcagtcta tgactgagat tgcccacaga ttggcggtct caacttccac
     1561 cgtcatccga aaactgaggg aatttaagtt tgaaaccgat tggaccaagt tgccaaaagt
     1621 tatgagttgg gatgagtata gcttcaaaaa gagcaaaatg agcttcattg cccaagattt
     1681 tgagtccaaa tccatcctcg caattttaga cgggcgaact catgcggtga ttcgaaacca
     1741 tttccaacgc tatcagagag aggttcggga gctggtcgag gtcatcacca tggacatgta
     1801 cagcccttat tatcggctcg ctaagcaact ctttccaaag gcgaagattg ttcttgaccg
     1861 cttccacatt gtccaacatc tgagccgagc tatgaaccga gtacgaatcc aaatcatgaa
     1921 ccaatttgac cgaaaatcct tggagtatcg ggcgctcaag cgcttttgga accctcgctt
     1981 tttcgtttct aggctcgggc taaatcagtc cactggactg atttactaca ccaggatagc
     2041 ttcaagctct gtcagaaacg attctatcag cccacgtttc gaatgcactt aacccatcgg
     2101 gaagtacgag ataagctgct ttcttactct gagggattac aggtttacta cgaactctat
     2161 caactcctgc tctttcattt tcaagagaag aatgccgacc atttctttgg attgattgag
     2221 caagaactgc caacggttca tccgcttttt caaacggtct tttggacttt tttaagggat
     2281 agagataaga ttatcaacgc acttaagctg ccttattcca acgctaaact tgaagcgacc
     2341 aataatttga ttaaggttat caagcgcaaa gcctttggtt tccggaactt taacaatttt
     2401 aaaaaacgga ttttgatgac tttgaacatc aaaaaagaga gtacgaattt cgtactctcc
     2461 agattgtagc ttttcgccta cccactacac ttgacaaaga gccactcttt attccatggt
     2521 atcaaaggca agacttggtt tggcattgag gtccaagttt gcaaagtttt ctttgttcca
     2581 ctcgctgacg ctggcatagg caatcatacc tgcattgtct ccgcagagtc gcagaggggg
     2641 gatgataacc ttgacatctg tgatttcggc tgctaggcgt tctctgagac ctttattggc
     2701 tgccacacca cctgccacaa ctagggtttt aacaggatat ttctccaaag ccttcttggt
     2761 ttttgccatg agaatgtcca taactgctgc ttggaaggaa gcacacaaat cttctgtaga
     2821 caggctttct cccttttgct cggcattgtg atgaagattg ataaaggcag atttcaaacc
     2881 tgagaaggag aactccagat tatcttcctt aatcatggca cgggggaaat cataaatatc
     2941 ctgcccctga tgagccagct cgtcaatctc acgacctgca ggataggtca agcccatgac
     3001 acgaccgacc ttgtcataag cctccccaac tgcatcgtct cgtgtctccc caacaatctt
     3061 gtaatcgcca gcctcagaaa catagaccaa ctctgtgtgc ccaccactga ctaaaagggc
     3121 tagcaaggga aactccaaag gctccacact ctgagctgcc atgaggtgcc cagccatgtg
     3181 attaacagga atcagtggaa gttcgtgagc ccaagcaaag gccttggcag ctgacaaacc
     3241 aactagcaag gctccgacca agcctggtcc gtaggtaacc gcaacagctg tcacgtcctc
     3301 ttcggtaatc cctgcttctg ccaatgcctc ctcgatacag gctgtaatga cctcgacatg
     3361 gtgacgactg gctacttcgg gcactacgcc accaaaacgt ttgtgactct caatttgact
     3421 agcaatgaca ttggacaaga gctcatcgtc gtttttcaag acggcgacac tggtctcatc
     3481 acaggatgtc tcaaatgcta aaatatatct atccttcatc tatttctctc ttcatgataa
     3541 tggcgtcctc gactgggtca tggtagtagg cctttcgctc agcgataact gccatctttt
     3601 ctttcttgta aaatgcttgc gctcgttgat ttgactgtct gacttcgagg aaaatttcct
     3661 tgtctgtcgg caattgagca aacaaggctg acgcaattcc ctgaccctga taagctcctt
     3721 tgacagcgat ttgcaggact tctgcttcaa aaagattctc ctgcacagct agaaatccaa
     3781 tcacttctgc cccatcataa gccaatgcat accaagtctg gtcttgggac agatctgctt
     3841 ggatttgctc cagagtccaa ggactgacta ggtaaacagc tgccataaca gcgtagatgg
     3901 cttgagctag gtcaggatgt tgttgaattc gcttgatttc tatcataggc gtttaatgta
     3961 ggactcacca gactcggtgt gattcttgag ccagttttcc tcagcttcga cacgcttgag
     4021 gtagttcggc acaaaatcat gcaaggagtc tgcttccttg tcccaagcta aaagagcaag
     4081 attggctgca ttgggcaatg tttctttgta atcagtcctt ggcaagtgtt tttgaatctg
     4141 ctcaacaaag gggccaactt ctccgacaaa ggttacctga ctagtaccct tgactttttc
     4201 tagcacctct tcaaaagata ggtgcgcttc tgccatgaca ggtttggcat tttcataaaa
     4261 tcctgcataa acattattgc gacgcgcatc catcaagggg acaaacaaac cttcttgttg
     4321 gtggggcacc agagccaaga gactcgacat accaaccaac tcgatgttca gggtgtaagc
     4381 taaggtctta gcagttgcta ccgcaattcg caagcctgta tagctacccg gcccttcagc
     4441 taccacgatt cggtccaaat ccttgggtgt ccaatccaaa cttgccatca aaaaatcgat
     4501 ggcaggcata agagtaatac tgtgattttt cttaatatta atcgtcgtct cggcaagaac
     4561 ctgcttatcc tctaaaatag ccagagaaag agccttgctg gacgtatcaa aagctaatac
     4621 tttcataaca cattcctatc tttttgtctg cttactatta tactacaaaa gctggcacat
     4681 gggaattttc tttgccccca gacaagagtg ccctcactta actaaaaata atttaaaaaa
     4741 atgctcactt ttccttttct tttccgaata taaaagtgaa caagaaaaaa ggaggaaagt
     4801 tcaatgacaa attttgacat tcttgacaat caatttttat ccttatctga aaatgaatta
     4861 tcagatattg atggcggtct cgctcccttg gttatctttg gagtagcagt atcttggaag
     4921 gctattgcag gtggaacagc acttataggt tctggtttgg cagctggtta ttttttagga
     4981 ggagattaat atgatgaaag atttgaacaa ttatcgtgaa atttctaata aggaattgca
     5041 agaaatcaag ggtggctttg gtgtcggtgt tggtatcgct ttatttatgg caggttatac
     5101 cattggaaaa gaccttcgta aaaagtttgg taagtcatgc tagataagaa acacattttt
     5161 agaaggataa attttattgt cttcatctct tacagtttgc tcagcattct caatgatttg
     5221 aacattacta ccatcccttt accattcgat ttatctgttt gtattgtttt atttttatgc
     5281 ttcaactcta tttttgatca gaacaatgac tcccataaaa ataataagct ttgaaaattc
     5341 cattgtcatg tcatgttaga aaaatgcaaa gaccacctca tcttgataga tggggtggaa
     5401 ttttcgtgtc gtaaatctac tatctctaca tccccaaaca aaaaacccca gcataagcag
     5461 ggcatctaag catttaattc aaagtaaaat acaaaccaaa cgacataggt cacgaggagg
     5521 agaaaaagcg agtagagagt cacaaaggtc attttccaca agaacttggt ttgtcgtcgt
     5581 tccagtttgg caaatagaag atttcctcca taaacgcaag caacaaaaac gataaaagct
     5641 agcaaacgaa ctccgatagc aaaagcaaat aagttataca tagggcaacc tccttgactt
     5701 aaaatctata tggaattatg acaagcaata aatttcactt ccgttatcaa cataatacat
     5761 tttctttatt tttgcaaacg cttaccaaag aaatcgtccc ctaactttct cgtttccgtc
     5821 ttttactaat ttttcatttt gtggtataat tgaaataatt gtaacgaatc aaggtcaatc
     5881 tagacacaaa atggaatgaa atcaagcaaa tctctgctaa aagtttggaa taagctgacc
     5941 tgtaaataga aaggaactat atgatttaca aagtttttta tcaagaaaca aaagaacgta
     6001 gcccacgccg tgaaacaaca cgcgcgcttt acctagacat cgatgccagc tcagaacttg
     6061 agggccgtat cactgctcgc caacttgtcg aagaaaatcg cccagagtac aatatcgaat
     6121 atatcgaact cttgtctgac aaattgctcg attacgaaaa agaaactggc gccttcgaaa
     6181 ttacggagtt ctaatatggc ctacactctt aaacctgaag aagtcggcgt ttttgccatc
     6241 ggtggtctag gagaaatcgg gaaaaacact tacggaattg aataccaaga cgagattatc
     6301 atcgtcgatg ctgggattaa attcccagaa gatgacttgc ttggtatcga ctatgtcatt
     6361 cctgactact cttacatcgt ggacaatatc gaccgcgtca aggctgtttt aatcacacac
     6421 ggacacgagg accacattgg tgggattccg ttcctactca agcaagcaaa tgtccctatc
     6481 tacgctggac cgcttgcctt ggctttgatc cgtgggaaac tcgaagaaca cggcctcttg
     6541 cgcaacgcca aactttacga aatcaaccac aacaccgagt tgacctttaa aaatctcaag
     6601 gcaactttct ttagaacgac tcactctatt ccagagcctt tggggattgt cattcatact
     6661 cctcaaggga aaatcgtctg tacgggtgac tttaagttcg actttactcc agttggagaa
     6721 cctgcggact tgcatcgtat ggctgcgctt ggtgaagaag gcgtgctctg tctcctgtct
     6781 gactcgacaa atgcggaagt accaaccttt accaactctg aaaaagtcgt tggtcagtcc
     6841 attatgaaga ttatccaagg tattgaagga cgtatcatct ttgcatcctt tgcctcaaat
     6901 atcttccgtc tccagcaggc aacagaagct gctgttaaga ctggacgcaa gattgcggtc
     6961 tttggtcgtt ctatggaaaa ggccattgtc aacggaatcg atcttggcta catcaaagct
     7021 cctaagggaa cctttatcga gccaaatgaa atcaaagatt atcctgcagg agaagttctt
     7081 atcctctgta caggtagtca gggtgagcct atggcagccc tctctcgtat cgccaacgga
     7141 acccaccgtc aagtacaatt acaaccaggt gataccgtta tcttctcttc tagtcccatc
     7201 cctggaaaca ctactagcgt caacaagctg attaacatca tttctgaagc tggtgtcgaa
     7261 gttatccacg gtaaagtaaa caatatccat acatctggac acggtggtca gcaagagcaa
     7321 aaactcatgc tctgcttgat taagccaaaa tacttcatgc ctgtccacgg tgaataccgc
     7381 atgcaaaaag tccacgctgg actagcagtg gatactggtg ttgagaagga caatatcttt
     7441 atcatgagca atggcgatgt gcttgccctt actgctgact cagctcgtat cgcaggtcat
     7501 ttcaacgccc aagatatcta tgtcgatgga aatcgtatcg gtgaaattgg cgcagctgtc
     7561 ctcaaagatc gtcgcgatct atctgaagac ggtgtcgttc tggcagtcgc aactgttgac
     7621 ttcaaatcgc agatgattct atctggtcca gacatcctca gccgaggctt tgtctacatg
     7681 agagagtctg gcgacttgat tcgccaaagc cagcgtatcc tcttcaatgc cattcgtatc
     7741 gcactgaaaa ataaggatgc tagcgtgcaa tctgtcaatg gtgccattgt caacgctatt
     7801 cgccccttcc tctatgaaaa taccgaacgt gaaccgatca tcatcccgat gatcctcaca
     7861 ccagatgaag aataatactc ttcaaaccac gtcagcttcg ccttgccgta tatatgttac
     7921 tgacttcgtc agttctatcc acaacctcaa aactgtgttt tgagctgact tcgtcagttc
     7981 tatccacaac ctcaaagcag tgctttgagc aacctacggc tagcttccta gtttgctctt
     8041 tgattttcat tgagtacaaa tcaacaaaac agccccgtct tcggagctgt ttttctctat
     8101 gctttctttt gagattaaaa ctcaatgaaa atcagactag aaggctaagc gaaagcataa
     8161 cttgagttag ctcccatagt tcggggaact atgggaggtt ggagatgaat caaagccaag
     8221 ctttgaactc attcgtaaga agccgacgac gtatcatttt gatttttgaa gagttttaga
     8281 aatactacga tttttacctt ccagatacac catcaaaata gaaatatctg ctgggtttac
     8341 tcccgaaata cggctggctt ggccgatggt ttctggattg atgagtttga acttctgacg
     8401 agcttccgtc gcaatagaat cgatgtcatc ccagtcgata ttggctggaa tgcgtttttc
     8461 ttccatacgt ttcatcttgg caacctgatc catggctttg gaaatatagc cttcgtactt
     8521 gatttctgtt tcaatcaatt cgataatctt gtcatccaag tcttctgcag ctggtccgat
     8581 gaaggccacc acatcttggt aagaaacttc tggacggcga aggaattctt tggctgtcac
     8641 cgcatctgtc aacggcttga agcccatttc ctcaacctta gcattggttt ccttgactgg
     8701 cttgagtttg atactgtcta ggcgtttcat ctcattatca aattgatttt tcttgatttc
     8761 aaaacgagcc cagcgttcat catccacaag gccaatctcg cgtcccatct cagtcaagcg
     8821 catatcagca ttgtcatgac gaagaatgag acggtattca gcacgacttg tcaagagacg
     8881 gtagggttca atggttccct tggtcaccaa gtcgtcgatc atcaccccga tataaccgtc
     8941 actgcgcttc aaaatcaact caggcttgcc ttggattttc agagccgcat tgatacccgc
     9001 gataatccct tggccagcag cttcttcata acctgatgtt ccatttgtct gaccagcagt
     9061 gaagagacct gagattttct tggtttccag agtcgcacgc aactgatgag gcaagaccat
     9121 atcatactca atagcataac ctgtccgcat catctctgca ttttccaaac ctttgatgga
     9181 atgaaccaag tcacgctgga catcctcagg cagactggtt gaaagtcctt gaacatagac
     9241 ttcctcagta ttgcgccctt ctggctcaag gaagagttgg tgacgttcct tgtccgcaaa
     9301 gcgcacaatc ttgtcttcaa tcgacggaca gtaacgaggc cccactccct tgaccacacc
     9361 tgtaaacata ggcgcacggt ggaggttgtt ttggataatc tcatgactgg taccattggt
     9421 ataggtcaac cagcatgaca cttggtcctt gacataatcc tcatcacgtg aagtgtatga
     9481 gaagtgatta ggcgcttcat ctcctggttg gatttctgtc acgtcgtagt tgatagaaga
     9541 ggccttgaca cgtggagggg ttcctgtctt gaaacgaccg atttcgagac ccagttcctt
     9601 gagattgtca gctaggttaa tagaagccaa gctgtggtta ggacctgatg agtacttgag
     9661 gtctccgatg ataatttccc cacggagagc agtccctgtc gtcacaataa cagccttagc
     9721 agcatattct tgatgggtgg ctgtacgcac accgacaacc ttgccatctt ccaccaaaat
     9781 ctcatcaatc atggtttgac gaagggtcag attttcttgg ttttcaaccg tcttgcgcat
     9841 ctccttagag taaagttcct tgtcagcctg cgcacgaagg gcacggacag ctggcccctt
     9901 ccctgtgttt agcatcttca tctggatgta agtcttgtca atggttttgg ccatctcgcc
     9961 accgagggca tcgacttcac gcacgacaat ccccttggca gaaccaccga tagagggatt
    10021 acaaggcatg aaagccagca tttcaatatt gatggtcgca agcaggacct tacagcccat
    10081 acggctagcg gccaaggaag cctcaacccc agcgtgtccc gcaccaatta caataatatc
    10141 gtattcttca gtaaaatgat aagtcatgtt tctctcctat tcttcaagat gaatgtgtct
    10201 tagttggcct tcccaatctg gtagggccgt ttttaaaaag gctggaacta gctggatatc
    10261 ctcaagctta tccaagtcaa tccactcaca gggctgcctt ttctcatctt cctgcatggt
    10321 caacggggca tcttcaagca aatccaccag ataatgaaac tcgatattgt gataggaaac
    10381 gccgtcttgt tcaaaacgat tttcaaccac aaaagctagc tgcccagctt gagatttaac
    10441 acccagttct tccttcactt cacggactac cgcgtcttcc gttttttcgt taacttgaat
    10501 cgcaccgccg ataatgtaat acttgccctt gtctttggtg actagaagct tgcgattttg
    10561 aagaatcaaa gctgtcgccc gaacaccaaa aaccgtattg tctacttttg tccgaaagtc
    10621 ttgttgagtc attcttgtcc tttcccttaa acgacacaaa aacagtcaaa actacaaaga
    10681 agtgcaggac aaaaaagcct gcaacatcca tgagctttga ccatcatttc tattgctgtt
    10741 attattgtag caaattgaga aaatttgtca atctaaatgt actgacaaac ttgtccatcg
    10801 cggtaagcat tgtcaatcaa accgccacct agacactctt cgccatcgta aaagacaact
    10861 gcctgtcctg gtgtaatcgc gcgttgtggt tccgcaaaga taacttctgc cttgtctcct
    10921 ttgacatgga cggtcacctt agagtcaggc tgacggtaac ggaatttagc cgtacattct
    10981 agcgtaaact cctcgggcat atcacgagta aagtggactt ggctagcctc taggctagtt
    11041 gacatgagcg aatcatggta gaaaccttgg cctacataga gaatattctt gctcaggtct
    11101 tttccgacaa cgaaccacgg cgcgttgtca ccaccgtgtt gtccaccgat accaagcccg
    11161 ccacgctgac caatcgtata gtacataaga cctgcatgct cgcccatatc gcgaccatcc
    11221 acagtcatca tgcgaccagg ctgagctggc aggtagttgc tgagaaagtt tttaaagttc
    11281 ttttctccga taaagcaaat ccctgtcgag tctttcttct tagcagtcga aagacctgct
    11341 tcttctgcta gttttcgaac ttcaggcttt tccaaatgtc ctagtgggaa catggttttt
    11401 tgaagttgtt cttgcgaaag ttggctgagg aaataggtct gatccttgcc attgtccacg
    11461 ccacgaagca tgtgaacggt accatcctca tcacgcacca ctcgagcata atgcccagtc
    11521 gctacatagt ctgcccccaa ggtcatagca tagtccaaaa aggccttgaa cttgatttcc
    11581 ttgttgcaca taacgtccgg atttggcgtg cgccctgcac ggtattccgc taggaaatac
    11641 tcaaaaacgc ggtcccagta ctctttttca aaattgacag agtagtaggg aatgccaatc
    11701 tggtctgcca ccgcagccac atccttgtaa tcttcggtcg ccgtacagac gccgttttca
    11761 tctgtgtcat cccagttctt catgaagata ccgatcacat cgtagccctg ctccttgagc
    11821 aaaagagccg tcaccgacga atcaacacca ccactcatcc ccacgacaac acgtgtttta
    11881 gagttatcac tcatggtaag tctcccatct attcgtttat tcacgattga aggtcgtgtg
    11941 tgcttcacga ttgaaggtcg cttgagcagt attcattata acacgcttgg ttagagaaga
    12001 caaggaagag cttaaaaatt gttttttaaa acttatagtc aatatacaga ttatgtgaat
    12061 gacgtgagta taccttcttc
//
LOCUS       DAVLYR010000049        11516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLYR010000049 DAVLYR010000000
VERSION     DAVLYR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.049"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            108..761
                     /locus_tag="VYO63_001033"
     CDS             108..761
                     /locus_tag="VYO63_001033"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1671969.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            771..1235
                     /locus_tag="VYO63_001034"
     CDS             771..1235
                     /locus_tag="VYO63_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1671970.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1248..2516
                     /locus_tag="VYO63_001035"
     CDS             1248..2516
                     /locus_tag="VYO63_001035"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1671971.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2530..2790
                     /locus_tag="VYO63_001036"
     CDS             2530..2790
                     /locus_tag="VYO63_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1671972.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2801..4162
                     /gene="pdrM"
                     /locus_tag="VYO63_001037"
     CDS             2801..4162
                     /gene="pdrM"
                     /locus_tag="VYO63_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1671973.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4316..5284
                     /locus_tag="VYO63_001038"
     CDS             4316..5284
                     /locus_tag="VYO63_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1671974.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5300..6292
                     /locus_tag="VYO63_001039"
     CDS             5300..6292
                     /locus_tag="VYO63_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1671975.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6510..7553
                     /locus_tag="VYO63_001040"
     CDS             6510..7553
                     /locus_tag="VYO63_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1671976.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7617..9302
                     /gene="lpdA"
                     /locus_tag="VYO63_001041"
     CDS             7617..9302
                     /gene="lpdA"
                     /locus_tag="VYO63_001041"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1671977.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9366..10355
                     /locus_tag="VYO63_001042"
     CDS             9366..10355
                     /locus_tag="VYO63_001042"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1671978.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10428..11498)
                     /gene="xerS"
                     /locus_tag="VYO63_001043"
     CDS             complement(10428..11498)
                     /gene="xerS"
                     /locus_tag="VYO63_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1671979.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3383 a         2175 c         2608 g         3350 t
ORIGIN      
        1 aaatcgtata tatagtcgat ttattaaatc tttagataaa ctgtaaaaag tggtttccat
       61 agccactttt tgctataata gaggcagtaa aaatgaagga gtcggctatg gaacactcgt
      121 cttggcatgc tttgattaag gcgcaattac ctgagggtta tttcgggaaa atcaatcagt
      181 ttatggagca ggtctattct caggggatta tttatccacc caaggaaaag gtttttcagg
      241 ctctcttgac aacactgctt gaagaagtta aggtggtaat tctagggcaa gacccctatc
      301 acggaccagg tcaagcgcag ggcttgagtt tttctgtacc tgactctatc ccagctccac
      361 catccttgca aaatatcttg aaagaattgt cagatgatat cggagttaag aaatctcatg
      421 atttgacagc ttgggctgag caaggagtct tgcttcttaa tgcttgtttg actgttcctg
      481 ctggacaggc caatggtcat gctggtcaaa tctgggagcc ttttactgat gctgtgattc
      541 aggtggtcaa tcatctagat agaccagtcg tttttgtact ctggggagct tatgcacgta
      601 agaagaaggc attagttacc aatcctcatc acttgattat cgaatcagcc catccaagtc
      661 ctttgtcggt ttatagagga ttttggggtt ccaagccctt ttccaaggcc aatgcattct
      721 taaaagagac aggacaagag ccaatcgatt ggcttagata aggagaaaat atgcctcagt
      781 tagcgacgat ttgctacatt gataatggga aagaactgct catgctccat cgtaataaga
      841 aacccaatga tgtccatgaa gggaaatgga ttggtgtggg tggtaagcta gagagaggag
      901 agacgcccca ggaatgcgcg gcgcgtgaaa tccttgaaga aacagggctc aaagccaagc
      961 cagttctaaa aggtgtcatc acttttcctg aatttacacc agatttagac tggtacacct
     1021 atgtttttaa ggtgacggag tttgagggcg acttgattga ctgcaatgag gggacgctag
     1081 aatgggttcc ctatgatgag gttttgagca agccgacttg ggaaggtgac cacacctttg
     1141 ttgagtggct tttagaggat aaacccttct tttcagccaa gtttgtttat gatggggata
     1201 aattgttgga tacccaagtt gatttctatg aataaaggag aaagcagatg ctactaatca
     1261 aaaatggtcg tgtaatggat cccaagtctg gtttggatca agtttgtgat gtcttagttc
     1321 aagatgggaa aattatcaaa attgcgtctg agatcacgga agaaggagca gaaacgattg
     1381 atgctactgg tcttgtggtt gctcctggct tggtcgatat tcatgttcat ttccgtgaac
     1441 ctggtcaaac acataaagaa gacattcata ctggtgccct agcagccgct gcaggtggtt
     1501 ttactactgt cgtcatgatg gctaatacca gtccaaccat ttcagacgtg gagactttgc
     1561 aagcagttct ccagtcagct gccaaagaga agattaatgt caagacagtt gcgaccatta
     1621 ctaaaaactt taatggtaaa aacttgactg actttaaggc actcttagaa gctggtgcgg
     1681 ttggtttctc tgatgacggt attccgcttg agagcagtaa gattgtcaag gaagccatgg
     1741 aggaagccaa aaaactcaat acctttatca gccttcatga ggaagatcca ggtttgaacg
     1801 gtgttcttgg ctttaatgaa aatattgcta gagaacattt ccatatctgc ggtgctactg
     1861 gggtggctga gtacgctatg atggcgcgtg atgtcatgat tgcctatgca actaaagccc
     1921 atgttcacat ccagcatttg tctaaggaag aaagtgttaa agtagtggag tttgctcagg
     1981 ggttaggtgc agaagtcaca gcagaagtag cgccacagca tttctctaag acagaagcac
     2041 ttcttttaac acaaggtagc aatgctaaga tgaatccacc gcttcgtttg gaatcagacc
     2101 gtcgtgccgt tatcgaaggt ctcaaatcag gtgtcatcac agttattgcg actgaccacg
     2161 cgcctcatca tgtagatgaa aaaaatgttg aggatattac caaagcgcca tctggtatga
     2221 ctggcttaga aacatccctg tctctcagct tgacctattt agtagaagct ggtgagttga
     2281 gcttgatgga attacttgaa aaaatgacat acaacccagc caagctttac aactttgaag
     2341 caggttactt ggctgagaat ggtccagcag atatcactat ttttgatgcc aaggctgacc
     2401 gctttgtgga ctcccatttt gcttccaaag cagctaattc accattcatc ggtgaaacct
     2461 taaaagggca ggttaaatat accatctgta agggacaaat cgtctatcaa gcttgatagg
     2521 agttagaata tgaattattt tcgaaaacgt agggagagac aagcgaaatc taatagtgga
     2581 atttactgtc ccgcagctaa tcgttccaaa attcaatatg aaactaaaga aaaagctgac
     2641 catgcggttc aatatgataa cggaggttta gttagaagct attattgtcg tacatgtgcg
     2701 tgttggcata ctacttcaaa atctaataaa cctccgctaa gtttgaaaaa ctatggtctg
     2761 aaactctttg gtttagataa atcagaatag aaagagaagt atgtataaga caaagtgttt
     2821 acgagagaag ttagtattat ttttaaaaat tttcttccca atcctgatct accaatttgc
     2881 caattattct gcctcttttg ttgatactgc aatgacaggt caatacaaca ctatggactt
     2941 ggctggtgta tctatggcaa ccagtatctg gaatcctttc tttacatttc taacagggat
     3001 tgtgtcagcc ttggtgccta tcattggtca ccatcttggt cgaggcaaaa aggaagaagt
     3061 tgcgtctgat ttttaccaat ttatttattt ggccttgggc ctatctgtgg tcttgctggg
     3121 gatggtactt ttcttggcac caataatctt gaatcatatt gggttagaag cagcagtagc
     3181 ggcagtagcg gttcgctatc tttggttttt atctatcggg attatcccct tgttgctctt
     3241 tagcgtcatt cgttccttgc tggattcgct gggcttgacc aaactgtcca tgtacctcat
     3301 gcttttgtta ctccctctca atagcggatt taactatctc ttgatttacg gtgcctttgg
     3361 tgttccagaa ctgggagggg ctggtgctgg tttaggaaca tccttggcct actgggtctt
     3421 gcttgggatt tctgttctgg ttttatttaa acaggagaag ctcaaagcct tacaccttga
     3481 gaaacgaatt ccacttaata tggataacat taaggaagga gttcgtttag gtctgcctat
     3541 tgggggaact gtcttcgcgg aagtggctat cttttcagtg gttggcttga ttatggctaa
     3601 gttttcgtcc ttgattatag ctagtcacca gtcagctatg aacttttcaa gtcttatgta
     3661 cgcctttcct atgagtatct catcggctat ggctattgtc gtttcctatg aagtgggagc
     3721 caagcgattt gatgatgcga aaacctatat tggtctagga agatggactg ctctcatttt
     3781 tgcggccttc accctaacct tcctttacat ttttagggga aatgtggcca gtctttatgg
     3841 taacgaccca aaatttatcg atttgacagc gcgtttttta acttatagtc ttttcttcca
     3901 gttagcagat acctttgcgg caccgcttca gggaattttg cgggggtata aggatacagt
     3961 tattcctttt taccttggtt tgcttggtta ttggggcgta gcaatccctg tggctacgct
     4021 atttgattcc ctaacagatt ttggagctta ttcttactgg atcggcttga ttattagttt
     4081 gattgtgagt ggggcgctct accgttggcg tttaactgtg attatgaaga gatttgaatc
     4141 tttagcaaaa tccaaacgct aaaaagtttg tgaaaaaata ttcttgacaa gaaaatccct
     4201 tgttttcacg gggttttctt ttttattttg tgaaatttct tttagaaaaa ctttgacagt
     4261 atttgccaaa agcggtaaaa tagtaaggta agaagaaagt tagaaaggca agaagatgtc
     4321 aactttagat aaaaatcttt tgctagaaat gttccgtaag atggaagaaa tccgtcgcat
     4381 ggacttaaaa attgcacaat tagtaaagaa agggaaagtg ccaggaatga cgcacttttc
     4441 tgttggtgaa gaggcagcta acgtgggggc tatgttagct ctcaatccag atgatctgat
     4501 tacctcaaac caccgtggac acgggcaagc tattgctaaa gggattgacc tcaacggaat
     4561 gatggctgaa atccttggta aatacactgg aacctgtaaa gggaaaggtg gatctatgca
     4621 tatcgctgac cttgatgctg ggaaccttgg tgccaatggt atcgtaggtg gtggtatggg
     4681 gatcgctgtc ggtgcagccc tcagtcagca aatgcaaaat accggtaaaa tcgttgtctg
     4741 cttctttgga gatggtgcga ccaatgaagg tgttttccac gaagcagtga acatggcttc
     4801 tatctggaac ctgccagtca ttttctattg cattaacaac ggttacggta tctctgcgga
     4861 tatcaagaaa atgaccaata tagaacatat ccatcaacgt agcgccgctt atggaattcc
     4921 tggaatgttc atcgaagatg gtaacaatgt catcgatgtc tatgaaggat ttcagaaagc
     4981 tgtggaccat gttcgcagtg gcaatggttc agtcttgatt gaaagtgtaa cttatcgctg
     5041 gctcggtcac tcatcatctg accctggtaa atatcgtacg cgtgaagaag tggaattgtg
     5101 gaaacaaaaa gatccaatcg aaaacctccg caattacctt attgaaaata acattgcaag
     5161 tgccgaagaa ttggaagaaa tccaagcgca agtaaaggaa gcagtagaag cttctgttaa
     5221 atttgcagag gaaagcccat tcccaccgct tgaatcagcc tttgaagata tttacgcaga
     5281 ctaaggagaa agagataaaa tggaaacaaa aacaatgtcc ttccgtgaca ccattatcct
     5341 tgctatgtct gaggaaatgc gtcgcgatga aaatgtgttc ttgatgggag aagatgtcgg
     5401 tgtcttcgga ggagacttcg gtacttctgt tggaatgctt gaagaatttg gtccagaacg
     5461 tgtccgtgac tgtccgattt ctgaagctgc catctctgga gcagcagcag gagcagccat
     5521 gacaggactt cgtccaatcg tcgatatgac cttcatggac ttctctgtta ttgccatgga
     5581 caatatcgtc aaccaagctg ctaaaacacg ttacatgttt ggtggtaaag gtcaggttcc
     5641 aatgactgtt cgttgtgcag ctggtaacgg agttggctct gccgcccagc actcacaatc
     5701 tctagagtct tggtttactc acattccagg acttaaggtt gtggcacctg gaacacctgc
     5761 ggacatgaaa ggcctgctta agtcttctat ccgtgataac aacccagtta ttatacttga
     5821 gtacaagtca gaatttaacc aaaaagggga agtgccagtt gatccagact acacaattcc
     5881 acttggggtt ggtgaaatca aacgccaagg aacggatgta acagttgtta cttatggaaa
     5941 aatgcttcgc cgtgtggttc aagctgctga agaattagca gaagaagaaa tttcagttga
     6001 aattgttgac ccacgtaccc ttgttccgct tgataaggat atcatcataa actcagtgaa
     6061 gaagactggt aaggttattc tggtcaacga tgcccacaaa acaagtggct atatcggtga
     6121 aatttcagct attatttcag aatcagaagc atttgactat ctagatgcac caatacgccg
     6181 ttgttcagga gaagatgtgc cgatgcctta tgcacaaaac ctagaaaatg caatgattcc
     6241 aacagttgaa agtatcaaag atgcaatccg aaaaacttat aacaaagact agagttacat
     6301 aatataagtt atgtaactaa aataaagctg acgagaaact ttgtatcttt taggtgcaga
     6361 gctcttcgtc attaatatct tatattcgag cacgggctaa aagctcggaa aaaagataaa
     6421 tctccttggc ttcatcgaag ccatcgtcga tttcctattt ttcagtcgct tttgacgccc
     6481 tttgtatcat aaatagaatt ggagaggtca tggctgatga caagctaaga gcgactcctg
     6541 cagctagaaa gttagcggat gatttaggga taaacctcta cgacgtttct ggctcaggtg
     6601 caaacggtcg tgtccacaaa gaagacgtgg aaacttataa agacacaaac gtggttcgca
     6661 tttcgccact tgcaaaacga attgccctcg aacataacat tgcttggcag gaaatccaag
     6721 gaaccggtca tcgtggtaaa atcatgaaga aggatgtttt ggccctgctt cctgaaaata
     6781 tcgaaaacga tagcatcaaa tctcctgctc agattgaaaa agtggaagaa gtccctgata
     6841 acgtaacacc atatggtgaa atcgagcgta ttccaatgac accaatgcgt aaggttattg
     6901 cccaacgcat ggttgaatct tacttgactg cgccaacctt caccctcaac tatgaagttg
     6961 atatgactga aatgttggct cttcgtaaga aggttcttga gccaatcatg gaagcaactg
     7021 ggaagaagac tactgtaaca gaccttcttt cgcttgcagt tgttaagact cttatgaaac
     7081 acccatacat caacgcttca ttgacagaag atggcaagac tattatcact cacaactatg
     7141 tcaatcttgc catggcagtt gggatggata atggattgat gacacctgtt gtttacaatg
     7201 ctgagaagat gagtctttca gaactggtgg tagcctttaa ggatgttatt ggccgtacct
     7261 tggatggtaa attggctcca agtgagttac aaaattcaac attcacaatc agtaatttgg
     7321 gaatgtttgg tgttcagtcc tttggtccga ttattaacca acccaactca gctatccttg
     7381 gtgtcagttc gacaatcgag aagccagttg tcgtcaatgg tgaaattgtg attcgcccaa
     7441 tcatgagttt aggattaacc attgaccacc gtgtcgtaga tggtatggct ggtgctaagt
     7501 ttatgaagga cttgaaagag ttgattgaaa cgccaatctc aatgttaatt taagagcaag
     7561 agtattcatt tttaagatat agataaagga aggaagacat ggccttagaa ataattatgc
     7621 caaaagccgg cgtggatatg acagaaggac aaatcgtcca atggaataaa aaagtcggag
     7681 aatttgtaaa agaaggagaa atccttttgg aaatcatgac tgataaagtc agcatggaat
     7741 tggaagccga agaagatggg tacttgattg ccattctcaa aggagatggt gaaactgtcc
     7801 ctgtaacgga agttatcggt taccttggtg aagaaaggga aaacatccca acagctggag
     7861 cagcatcgcc agaagctagc cctgtacctg tagcaagtac ctcaaacgat gatggtaaga
     7921 gcgatgatgc ttttgatatc gttgtgattg gtggaggtcc tgctggttat gttgcagcca
     7981 ttaaagctgc ccaactcggc ggtaaggttg cccttgttga gaaatctgaa cttggtggaa
     8041 cctgcttaaa ccgtggatgt attccaacca agacctacct tcatagcgct gaaattattg
     8101 aaaatatcgg tcatgctgca aatcgtggta tcgtgattga aaatcctaac ttcactgttg
     8161 atatggaaaa acttttagaa actaaatcta aagttgttaa tactcttgtt ggtggtgttg
     8221 caggacttct tcgtagctac ggagttgctg ttcataaagg aattggtaca atcactaaag
     8281 acaagaacgt cttggtaaat ggttctgaat tgcttgaaac caagaaaatc attcttgctg
     8341 gtggttcaaa agtcagcaag atcaacgtcc ctggtatgga atctccactt gtcatgacta
     8401 gtgatgacat tcttgaaatg aacgaagtgc cagaaagcct tgttatcatc ggtggtggag
     8461 ttgtcggtat cgaactcggt caggccttca tgacatttgg ttcaaaagtg actgttatcg
     8521 aaatgatgga ccgtatagtt ccagctatgg atgtggaagt ttctaagaac cttcgcttga
     8581 tccttgagcg taaaggaatg accatcttga ctggtactaa actgcaagaa atcattgagg
     8641 aaaatggtca acttcgtatc aaggttgaag gaaaagacga tatcatcgca agcaaagctc
     8701 ttctttcaat tggtcgtatg ccagaccttg aaggtattgg agaggttgag tttgaattgg
     8761 atcgtggttg tatcaaggtc aacgaataca tggaaacttc agttccaggc atttatgcac
     8821 caggtgacat caacggtact aagatgttgg ctcacgcagc tttccgcatg ggtgaagttt
     8881 ccgctgaaaa tgcccttaaa ggaaatcatg cagttgccaa attgaatttg actcctgcag
     8941 ccatctacac tcttcctgaa gtagcagcag taggtttgac agaagaacaa gcccgtgaga
     9001 aatacgatgt tgccatcggt aagtttaact ttgctgctaa cggtcgtgct attgcatctg
     9061 acgcagctca aggtttcgta aaagttatcg ctgataagaa atacggagaa atccttggtg
     9121 tacacattat tggtcctgca gccgcagaat taataaacga agcatcaagc atcatcgaaa
     9181 tggaaatcac tgttgaggaa atgctgaaga ccatccacgg gcacccaact tactctgaag
     9241 tgatgtacga agcgtttgca gatgttctag gaatggccat ccattcacct aagaaaaaat
     9301 aaaacgaaga tagattagac tggaaggtta tttccagttt ctatcatata aagtaaaggg
     9361 atatcatgaa atacattatc aatcattcaa atgacactgc ttttaatatt gccttggaag
     9421 aatacgcctt taaacacctt ttggatgagg atcaaatctt cctactttgg attaacaagc
     9481 catctatcat tgttggtcgt caccagaaca ctattgaaga aatcaaccgt gattatgttc
     9541 gcgaaaatgg tattgaggta gttcgccgta ttagtggtgg tggagctgtt taccacgatt
     9601 taaataacct caactacacg atcatctcca aagaagatga aaataaggca tttgacttca
     9661 agagcttctc aactccggtt atcaatacct tggctcaact cggggttaaa gctgagttca
     9721 caggtcgtaa tgaccttgag attgatggta agaagttctg tggcaatgcc caagcctata
     9781 tcaacggccg tatcatgcac cacggttgct tgctctttga cgttgatttg tcagtcctcg
     9841 caaatgccct taaggtttca aaagataaat ttgaatcaaa aggtgtgaaa tccgtccgtg
     9901 cccgtgtaac caatattatc aatgaattac caaaaaaaat cacagtcgaa aaattccgtg
     9961 atttactctt ggaatacatg aaaaaagagt acccagagat gactgaatac gtcttttcag
    10021 aagaagaatt ggccgaaatc aatcgcatca aggatactaa gtttggaact tgggactgga
    10081 actatggtaa atcacctgaa tttaacgtcc gtcgtggaac caaattcact agtggtaagg
    10141 ttgaagtctt tgctaacgtt actgaatcaa aaatccaaga catcaagatt tatggtgact
    10201 tctttggtat tgaagacgtt gctgcagtag aagctgtcct tcgtggggtg aaatacgaac
    10261 gcgaagatgt tcttaaggcg ctaaaaacca ttgatatcac acgctacttc gctggtatta
    10321 gccgtgaaga aatcgctgaa gcggtagttg gataaatcaa aaagaagttg gccaactaac
    10381 caacttcttt ttttgatatt tatttacata atttaaaata cgtaaaatca taaactatcc
    10441 agagcattct tttgttcatc actaacaata tgggtataga ggtcagtgac ttgtgtgcta
    10501 gcatgtccta gttggtgact gactaaaact tgtgatttag tcgcatcata gagcctagtt
    10561 gctagtgtat ggcgcagttt atggggtgtt acacgcactt taaaatcctc tgagtattta
    10621 gcaaccattt tctcaacgct agaagcatcg atacgattag gaacacctct gtagagagtt
    10681 aaaaaaaggg ctgtatctgt tttttccgtt ttatagcgtt gattccgaat ggccagataa
    10741 ttctctaaat aaggtttagc aaaagcagcg acattgactg agtcacgttt gccacctttt
    10801 cgagtaacat caataaccat catttttaga ttgagatctc ttagatctag attaacagct
    10861 tcagataagc gaacaccaga tgccaagaga agggcaataa tggctaaatc tcgttcttta
    10921 tttttgttga atgatgagag agctcgattt gaaagctgtt gtgggtgctc ttgatcgata
    10981 taagttagaa aaccttctgt ttcatcacct agaaagagtt tttgcttgat attttcagct
    11041 ctggcagcaa gggtttcttt ctttttcttg gttgaaactt ttttcattac attacgatag
    11101 aaataaggtt ccccctgatc gttttcaacc tcctcggtta gatacttgta aagactagaa
    11161 agtgctgata aggttcgatt gatagttgtc tgtgaaacac cttgttttgt tgtattagca
    11221 ttcagcaagg gacgttcacg tagataaagg ataaaggatt ccatgtcttt cttagacata
    11281 ttttccaaaa ctgataaagg aatatcggat attttatcag cgtttgaaat accagactcc
    11341 aaaacccagc tgaaaaatcg gtcatattcc ttaaggtatt cgtacagggt tgtaaaactg
    11401 tagggcacag ccagtttaga ttggtagtat tccagaacat accagggcat gagttgtttt
    11461 agtttgtcga ttcgttccag taaaatctca cgtttcatgt attttctcca taatta
//
LOCUS       DAVLYR010000050        11398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLYR010000050 DAVLYR010000000
VERSION     DAVLYR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.050"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            17..577
                     /locus_tag="VYO63_001044"
     CDS             17..577
                     /locus_tag="VYO63_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1671980.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            579..1478
                     /gene="htpX"
                     /locus_tag="VYO63_001045"
     CDS             579..1478
                     /gene="htpX"
                     /locus_tag="VYO63_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1671981.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(1627..3186)
                     /locus_tag="VYO63_001046"
     CDS             complement(1627..3186)
                     /locus_tag="VYO63_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1671982.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(3425..3742)
                     /locus_tag="VYO63_001047"
     CDS             complement(3425..3742)
                     /locus_tag="VYO63_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1671983.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            3912..4454
                     /locus_tag="VYO63_001048"
     CDS             3912..4454
                     /locus_tag="VYO63_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1671984.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            4454..5083
                     /gene="nth"
                     /locus_tag="VYO63_001049"
     CDS             4454..5083
                     /gene="nth"
                     /locus_tag="VYO63_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1671985.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            5294..5815
                     /gene="pyrR"
                     /locus_tag="VYO63_001050"
     CDS             5294..5815
                     /gene="pyrR"
                     /locus_tag="VYO63_001050"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1671986.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            5834..6757
                     /locus_tag="VYO63_001051"
     CDS             5834..6757
                     /locus_tag="VYO63_001051"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1671987.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            6807..7886
                     /gene="carA"
                     /locus_tag="VYO63_001052"
     CDS             6807..7886
                     /gene="carA"
                     /locus_tag="VYO63_001052"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1671988.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            8199..11375
                     /gene="carB"
                     /locus_tag="VYO63_001053"
     CDS             8199..11375
                     /gene="carB"
                     /locus_tag="VYO63_001053"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1671989.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         3267 a         2254 c         2518 g         3359 t
ORIGIN      
        1 agaaaggact tatattatga cttggattat tcttggagtt atcgctctta ttgttatttt
       61 tgtgattgtt agctataacg gtttggttaa aaatcgtatg caaaccaagg aggcttggag
      121 tcagattgat gttcagttga aacgtcgaaa tgacctcttg ccgaacttga ttgagactgt
      181 aaaaggttat gccaaatatg aaggttctac ccttgaaaag gtggcagaac tacgtaacca
      241 agtggcggca gcgacttcac cagcagaagc tatgaaagcc agtgatgccc tcactcgtca
      301 ggtttcaggt atttttgcag ttgcagaaag ctatccagat ttgaaagcta gtgctaactt
      361 tgttaaattg caagaggagt tgacaaacac agaaaataaa atttcttact ctcgtcaact
      421 ctataacagt gttgtcagca actacaatgt aaaattagaa actttcccga gcaatattat
      481 cgctggaatg tttggattta aagcggcaga tttccttcaa acacctgaag aggaaaagtc
      541 ggttcctaaa gttgatttta gcggtttagg tgactaagat gttgtttgat caaattgcaa
      601 gcaataaacg aaaaacctgg attttgttgc tggtattttt cctactctta gctcttgttg
      661 gttatgcggt tggttatctc tttataagat ctggacttgg tggtttggtt atcgcactga
      721 ttatcggctt tatctacgct ttgtctatga tttttcaatc gacagagatt gtcatgtcca
      781 tgaatggagc gcgtgaggtg gatgagcaaa cggcaccaga cctctaccat gtagtggaag
      841 atatggctct ggtcgcccag attcctatgc cccgtgtttt catcattgat gatccagcct
      901 taaatgcctt tgcgacaggt tctaatcctc aaaatgcggc tgttgctgcg acttcaggtc
      961 tactagctat catgaatcgt gaagaactag aagctgttat gggacatgaa gtcagtcata
     1021 ttcgtaatta tgatatccgt atttcgacta ttgcagttgc ccttgctagt gctatcacca
     1081 tgctttctag tatggcaggt cgtatgatgt ggtggggtgg agcaggtcgc agacgaagtg
     1141 atgatgaccg agatggaaat ggtcttgaaa tcattatgct agtggtttcc ctactagcta
     1201 ttgtactggc acctctcgct gcaaccttgg ttcagctcgc tatttctcgt cagagggaat
     1261 ttctggcaga tgcatctagt gtcgagctga ctcgcaatcc ccagggaatg attaatgccc
     1321 tagataagtt ggacaatagc aaacctatga gtcgccacgt cgatgatgct agcagtgctc
     1381 tttatatcaa tgatcccaag aaaggtgggg ggttccaaaa actcttttat acccacccac
     1441 ctatctcaga acggattgaa cgtttaaaac agatgtaaaa tgaaggctgg aaaaaagtct
     1501 ttaaaatctg aaaaacgcat aatatcaggt gtgaaaactt gatattatgc gttttactat
     1561 gggaagattt acttcttttt ctcctaaaat tgtgtttttg ccccacctat ctgctatgtt
     1621 gcaaatttat aaatcttcta aattaactag cttcaaaccg tgttctgtca tgcgatagat
     1681 gatcgagcag atttctttta agaaacgacg gtcatgcgaa acagtgatga gaccgcctgg
     1741 ataggttgca aagagttttc tgatttgggg ttgagaagtg ggagaaaagt ttcgtgtggg
     1801 ttcatccagc aggagaaagt ttggtttgcg caggactaaa tccaaaagca ggagttttcc
     1861 ctgttgtccg ccagataagg agcgaatttg atgctgcatt tctggataac tgaaattgag
     1921 actggctagg tgagattgga ttttctgaag ttcctctttt tccccagttt tgctgatata
     1981 agctattggt gataaatcca attgcagttt tttgtggtaa tcttgtggca taaaaccaag
     2041 ggaaatctct cttttatcat tcagaagttg ctttaatttg gctaacagag ttgatttccc
     2101 aacaccattt ggcccgataa taccgatttt ttcttgccca cggacagtta gttgtagttt
     2161 ttgaaccaaa actcggtcgt caatggacaa attttctttt tccagttgga ctaagacttt
     2221 agaagctggt aatggttgga tgtctgaaaa gaaaagttgg atttgttcct cttcaagtgg
     2281 cttttgagtc atggactgag ctgtcttttc gtagcgtttt tcttgtgaga ggacagtttt
     2341 catcttttta gccaataggc gaccggcagt actatctttg gtagctcgaa gcgcagtttc
     2401 tacattttgc ttaactctcc gatgtttttc catggtttta tcgtaggctc tttggttgtt
     2461 agcagcttgc tgactttgtt tggcaaaatt agcctttctc tgctcactat agctatcata
     2521 gtctaaatgc tctactagcg tttccgcttc tttacggtgt ttgaccagtc gcaagtgaac
     2581 aatagtgtct gctgtttcag aaagaaagtc ttcatcatgg gaaatgaaaa taacggtttg
     2641 cctggtcttt tgaatctggc cttttagcca atcaactgtc tcaaggtcta ggtcatttga
     2701 aggttcatct aaaaatagaa tctcaaaggg tttggctaac tcatggataa gctgaatttt
     2761 caaagcttct ccccctgata gattgccaat cttttggtca cttgcgaaac gattgctatc
     2821 aaaatgcaat tcctccgcca aacgatagag gatactgtag tctaaatcaa tagaatctaa
     2881 aaagaagtag tcgtgtaaag ttttcttttt cagctcctcg ggaagttttt gaggaatgta
     2941 ggccagtgac tgatagtcag attggatgtt tcccttgata gtgaaatcag acaaagcttc
     3001 ccccattaaa attttaagta aggttgattt gccatttcct tcttcaccaa taatagcaac
     3061 cttttccccg tcttggatgg tcatgcttag gtcagataca agatctcgta aatctttgtt
     3121 ttgtgtgatg gttagatgat tgatttttat catatgattt ccctttctag aatgttcata
     3181 gtgcataaca aaaagctcta ctttacctag tagagcccaa agtctatgtc aaaaaaactc
     3241 tattaacatg ccagaaaaag agcataaaag agcacacaaa aagagacaaa aacaagatct
     3301 actaaataaa ggttctttat ttgatagact tagttgttca tgtctccctt acctccgagt
     3361 attagtatct ttatattata cctttaagga aattttgtca acagaaaact ggaaattcta
     3421 tgctctaaaa tccaaaaaca ggtccataga gacttagtac gtgtttgaca tgctcgtaat
     3481 ggaatttatc atagtttcgc caagcagtgc gtgcttgatc gtttagtttt aggctaccca
     3541 gtccaatcag aagactggtg ggttggtaaa atttctcaag atcaattaag atactgttgt
     3601 tcaacttttc cgtttttttg agctcttgca tagtagtgtt tagcagctct tgtagtcgaa
     3661 tatcatctgc cgtaccctgt ttacagtttt ggtagctttt atttaacttg gataggagtt
     3721 gaatgagttt cttgtcttcc atgtgagcat cctccttgat ctgatgtact ctttccctat
     3781 agtatagcac taaaaaacaa aactgtcata gtaaatatgt taaaattctg aattttaatt
     3841 acaaagtttg gtgtgctgat ttgtagcctt ttcatggtat aatcaagaga gtaaatcttt
     3901 atggaggaag tatgaaatta aatattcaag aaattcgtaa gcagtctgaa ggtttgaact
     3961 ttgaacaaac gttagattta gttgatgacc tgcgtgcacg taatcaagaa attttagatg
     4021 taaaagatat ccttgcagtt gggaaagtac aatatgaaga ccgtatgtat ttcttagatt
     4081 atcaactatc ttataccatt gttcttgctt cgagtcgcag tatggagcca gttgagttag
     4141 ttgaatctta tccagtcacg gaagttttca tggaaggcgc aactaaccat ctaaatcaag
     4201 aaattttaga tgatgacttg gtcttgccca tcgaaaatga ggcgcttgac cttgctgaga
     4261 gtgtatcaga caatatcttg ctaaacattc ctatcaaggt cttgacggct gaagaagaag
     4321 ctggtcaagg atttgtctca ggaaatgact ggcaaatcat gacagaggaa gaataccaag
     4381 ctcaacaagc agtaaagaaa gaagaaaaca gtccttttgc tggcttacaa ggactatttg
     4441 acggagatga ataatggtct tgtcaaaaaa acgagcacga aaggtgctag aagaaatcat
     4501 tgccctcttc ccagatgcca agcctagtct tgattttacc aatcattttg aactcctggt
     4561 tgcggtcatg ttatcagccc agacgacaga tgcagcggta aataaggcca caccaggtct
     4621 ctttgttgcc tttccaacac cccaagctat gtctgtagcg acagagagtg agattgcttc
     4681 acatatttct cgcttgggat tgtatcggaa taaagctaaa ttccttaaaa aatgtgccca
     4741 acagttacta gacgattttg atggtcaagt ccctcagaca cgtgaagaat tggagagttt
     4801 ggcaggtgtt ggtcgcaaga cagccaatgt tgtcatgagt gtaggatttg ggattccagc
     4861 ctttgcagtt gatactcatg tggagcgtat ttgcaaacac cacgatattg tcaaaaaatc
     4921 agcgacgcca cttgaggtgg aaaagcgggt catggatatc ttgccgcctg agcagtggtt
     4981 agctgcccat caggccatga tttattttgg aagagccatt tgccatccaa aaaatccaga
     5041 gtgtgaccag tacccacaat tatatgattt tagcaatttg taagatgcta cattgaaact
     5101 ttttgcttga ttttaagcat gctttgtgct atagtaaatg ataactaaat attcctttaa
     5161 acctgtcccg tgaggcaggc aaggagcgcg ataaagtaga agggtgaagt ctatactgtg
     5221 tgcagtaggg ctcgcttggc tataaatttc aagtctcctt gtttaaggag acttttcttt
     5281 ttggaggttt gtcatgaaaa caaaagaagt tgtagacgaa ttgaccgtca aacgagcgat
     5341 tacgcgtatt acttatgaga ttatcgaacg caacaaagat ttgaataaga tcgtcttggc
     5401 tggtattaaa actcgtggtg tctttattgc ccaccgaatc caagaacgtt tgaagcagtt
     5461 agaaaatctt tcagttcctg ttgtggaatt ggatactaaa cctttccgtg atgatgttaa
     5521 aagtggagaa gatacttctt tggtttctgt cgatgtgaca gaccgcgaag ttatcttggt
     5581 ggatgatgtg ctctatacag gtcgtaccat ccgtgctgct attgataata ttgtcggtca
     5641 tggtcgtcct gcgcgtgtga gtttagcagt tctagtcgat cgtggacata gagaattgcc
     5701 aatccgtcca gattacgttg gaaaaaatat cccaaccagt cgttctgaag aaatcatcgt
     5761 agagatgaca gaacttgatg accaagacag agttctgatt actgaagaag cttagaaagt
     5821 taaaggagta gccatgtcag aaaatcaaca agcattgaac catgtggtgt ccatggaaga
     5881 cctcactgtc gatcaagtaa tgaaattgat caagcgagga attgagttta aaaatggagc
     5941 ccagcttccc tatgaagacc atccgattgt ttccaatctc ttctttgagg attctacacg
     6001 gacacataag tcctttgaag tcgcagagat taaacttgga ttggaacgac ttgactttga
     6061 tgtgaagact agttcggtta ataagggtga gacactttat gacaccattt tgactctgtc
     6121 tgctttagga gtggatgtct gtgtgattcg ccatccagag gtcgactact atagagaatt
     6181 gattgctagt ccaacgatta cgacttccat catcaatggt ggagatggtt cgggccaaca
     6241 ccctagccag agcttgcttg atttgatgac catttatgag gaatttggcc actttgaggg
     6301 tcttaaagtt gctattgcag gtgacttgga ccactcacgc gttgccaaat ccaatatgca
     6361 gattttgaaa cgcttgggat ctgaactctt tttcgctgga cctgaggaat ggagaagtca
     6421 agagtttgca gactatggac agtttgtaac cattgatgaa atcattgatc aggttgatgt
     6481 catgatgttt ctccgtgtgc aacacgaacg ccatgatagt ggagctgtat tttcaaaaga
     6541 agactaccat gcccaacatg gcttgactca agaacgttac gatcgtttga aagaaacagc
     6601 aatcctcatg cacccagctc caatcaatcg tgatgtagaa atagcagacc acttggttga
     6661 agcaccaaaa tcacggattg tccaacaaat gaccaatggt gtctttgttc gaatggcaat
     6721 cttagaatcc gtactagcga gtagaaacgc caactaaaac acaagacgtt aaaagacgcc
     6781 agtgagcgtc cacgagaggt gaaaatatga caaaaagaat tctagtatta gaagatggca
     6841 cagtttttga aggcaaggcc ttcggagcag atattgatgt aacaggcgaa atcgtcttta
     6901 atacagggat gaccggctac caagaatcca ttacagacca gtcttataat ggacaaatct
     6961 tgacctttac ttatcctttg gtaggaaatt atggtattaa tcgtgatgat tatgaatcca
     7021 ttattccaac ttgtaaggga gtcgttgttt ttgaagaagc gcgtagagct agcaactggc
     7081 gcaaccaaat gaccttggat gaatttttga aagccaagaa aattccaggg atttcaggaa
     7141 ttgatacgcg tgctcttacc aagattatcc gtaagcatgg tactatgcgt gcaaccttga
     7201 cccatgttgg ggacagtatg gaccatgtga cggaccagct ccaagcaaca gttttaccga
     7261 cagacaatat caaacaggtt tctactaaaa cgtcttatcc agctccagga gttggtttga
     7321 gcgtggtgct agtggacttt ggtctcaagc actcaatcct acgtgaactt tctaaacgca
     7381 actgtaacgt gacagttgtt ccttattcga caacggctga agaaattctc catctcaatc
     7441 ctgacggagt tatgttgtca aatggtccag gtaacccaga agacgttcca caagcactcg
     7501 acatgattcg cggtgtacaa ggaaaaattc caatctttgg tatttgtatg ggacaccaac
     7561 tctttgcaat ggcaaacggg gctaagacct ataagatgaa gtttggccac cgtggattca
     7621 accatgcggt acgtgaaatc gcaacaggac gagtagactt caccagccag aaccatggtt
     7681 atgcagtcag ccgcgaggat ttgccagagc acttgattat tacccacgaa gaaatcaatg
     7741 acaagtcagt ggaaggtgtg cgccacagat accaacctgc tttctctgtt caataccacc
     7801 cagatgcagc tcctgggcca cacgacgcta gctacctatt tgacgagttt atcgagatga
     7861 tggaagtttt taaacaatca aactaagaac gagtaaaagc tcgtcggaga atttatgtaa
     7921 ctgaacacgg acggaaagct cagaatgtag agaaaccaac ttggattgtc aatccttcct
     7981 tgatttccta aaattcaatc gctttctatt cgtcctttgt atcttattta atgtcgccct
     8041 gagaggcgac gaatagaaag gagaagggag ctccgtattt gctgaactga atacgggcta
     8101 cggacggtgc taaaaagata gttattccta gaactgacag ttcttcgtca taactcctat
     8161 tttagctttg tccgcttgac acccttaata tcttatatat gcctaaacgt actgatattc
     8221 aaaaaattat ggtgattggt tctggtccga ttattattgg tcaggctgct gagtttgact
     8281 acgctgggac tcaggcttgc ttgtcgttga aagaggaagg ttatgaggtt gttttggtta
     8341 actcaaatcc tgcaaccatc atgacggaca aggaaattgc tgacaaggtt tacattgaac
     8401 cgattacact tgagtttgtg acacgtattc ttcgtaagga aggtccagat gccttgctac
     8461 caacactcgg tggtcagaca ggtctcaaca tggccatgga attatctaaa aatggtatcc
     8521 tagacgagct tggcgttgaa cttctgggta ccaaattatc tgccattgac caagcggagg
     8581 accgtgatct ctttaaacaa ttgatggaag agcttgaaca acccattcca gaatctgaaa
     8641 ttgttaacac agtagaagaa gcagttgcct ttgcagcgac aattggctac ccagtcatcg
     8701 ttcgtccagc ctttaccctt ggtggtactg gtggtggtat gtgtgccaac gagaaagaat
     8761 tgcgtgaaat cactgaaaat ggtttgaaat tgtcacctgt tacccaatgt ttgattgagc
     8821 gttcgattgc cggtttcaag gaaattgaat acgaagtgat gcgtgactca gctgacaatg
     8881 ccttggttgt ttgtaacatg gaaaactttg acccagttgg gattcacaca ggggattcca
     8941 ttgtatttgc ccctgcgcaa accatgtcag actatgaaaa ccaaatgctt cgtgatgcga
     9001 gcttgagcat tattcgtgcc ctcaagattg aaggtggatg taatgttcag ctagctcttg
     9061 atcctaacag cttcaagtat tatgttatcg aagtaaaccc tcgtgtatca cgttcgtcag
     9121 cccttgcttc taaagcgaca ggatatccga ttgctaagtt ggctgccaag attgcagtag
     9181 gtttgacctt ggatgaggtt ataaacccag ttacaggttc aacctatgcc atgtttgaac
     9241 cagcccttga ctacgtagtt gctaagattc cacgtttccc atttgacaag tttgaaaagg
     9301 gtgaacgccg tctcggtaca caaatgaagg cgactggaga agtcatggca atcggtcgta
     9361 acatcgagga atctcttctc aaggcctgcc gctcccttga aattggggtg caccacaatg
     9421 aaatacctga acttgcagca gtttctgatg ataccttgat tgaaaaggtt gtcaaagccc
     9481 aagatgaccg tctcttctac gtatcagaag ccattcgtcg tggctacaca cctgaagaaa
     9541 ttgcagagct tacaaaaatt gatatcttct atcttgataa actcttgcat atctttgaaa
     9601 ttgagcaaga attgggtgct catccacaag atctagaagt tttgaaaaca gccaaactta
     9661 atggtttttc agaccgtaag attgctgaac tctggggaac gactgatgat aaagttcgcc
     9721 aacttcgctt ggaaaacaag attgttccag tttataagat ggtagacact tgtgcggcag
     9781 agttcgattc cgaaacgcca tatttctatt caacctatgg ttgggaaaat gaatctatca
     9841 aatctgataa agaatctgta cttgtcctag gttcaggtcc aatccgtatc ggtcaagggg
     9901 ttgagtttga ctacgcaacc gttcactctg ttaaggctat ccaggcagct ggttatgaag
     9961 ctatcatcat gaactcaaac ccagagaccg tttcaacaga cttctctgta tcagataagc
    10021 tttactttga gccattgaca ttcgaagatg ttatgaacgt cattgacttg gaacaaccaa
    10081 agggagttat cgttcagttc ggtggtcaaa cagccatcaa ccttgcagag ccattggcaa
    10141 aagcaggtgt gaccatcctt ggtacacagg ttgctgacct agaccgtgcc gaagaccgtg
    10201 acctcttcga gcaagctctc aaagaattgg atattccaca gccaccagga caaacggcta
    10261 ccaatgaaga agaagcagct cttgcagctc gtaagattgg cttcccagtt cttgtccgcc
    10321 catcttatgt acttggtgga cgtgccatgg aaatcgttga aaacgaagaa gaccttcgtt
    10381 cttacatgcg aactgctgtt aaggctagtc cagaccatcc agttcttgtc gactcttata
    10441 tcgttgggca agagtgcgaa gttgatgcca tttcagatgg taaaaatgta ctcattcctg
    10501 gtatcatgga gcatatcgaa cgtgctggtg tccactcagg tgactcaatg gctgtttacc
    10561 caccacaaac cttgtcgcaa aaggttcagg agaccatcgc agactacacc aaacgcttag
    10621 caatcggtct tcactgcctt ggaatgatga acatccagtt tgttatcaag gatgaaaaag
    10681 tctacgttat tgaggtcaat ccacgtgcca gccgtaccgt tccattccta tctaaggtaa
    10741 ccaatattcc tatggctcaa gtagcgacca agctcattct tggtcaaagt ttatcagaac
    10801 ttggctacca aaatggactt taccctgaaa gcacccgtgt tcatatcaag gcacctgtct
    10861 tctcctttac caaactagct aaggtagaca gcttgctcgg tcctgaaatg aagtcaacag
    10921 gtgaagttat gggttctgat gctactttgg aaaaagctct ctataaagcc tttgaagctt
    10981 cttatctaca cttgccaact tttggtaatg tcgtctttac catcgctgat gatgccaaag
    11041 aagaagcctt gaacttggct cgtcgtttcc aaaacattgg ttatggaatc cttgcgacag
    11101 aagggacagc agccttcttt gctagtcatg gcttgcaagc ccaacctgtt ggtaagattg
    11161 gtgatgacga taaggatatc ccaagttttg tacgcaaagg aagaattcaa gctatcatta
    11221 acacagtagg aacaaaacga actgctgacg aagatggtga gcaaattcgt cgttcagcca
    11281 ttgaacacgg ggtgcccctc ttcacagcgc tagatacagc taatgccatg cttaaggtac
    11341 ttgaaagtcg tagctttgtt acagaagcga tttaaataga aaaataagat atagcaga
//
LOCUS       DAVLYR010000051        11128 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLYR010000051 DAVLYR010000000
VERSION     DAVLYR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.051"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..234
                     /locus_tag="VYO63_001054"
     CDS             <1..234
                     /locus_tag="VYO63_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1671990.1"
                     /translation="YNQDKNMCKQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKL
                     IQKGFSLLELTEETSILFHLKKEVEIYYQAKER"
     gene            complement(523..993)
                     /locus_tag="VYO63_001055"
     CDS             complement(523..993)
                     /locus_tag="VYO63_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV1671991.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(959..1765)
                     /locus_tag="VYO63_001056"
     CDS             complement(959..1765)
                     /locus_tag="VYO63_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1671992.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(1981..2622)
                     /locus_tag="VYO63_001057"
     CDS             complement(1981..2622)
                     /locus_tag="VYO63_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1671993.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(2619..4727)
                     /locus_tag="VYO63_001058"
     CDS             complement(2619..4727)
                     /locus_tag="VYO63_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1671994.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(4779..5066)
                     /locus_tag="VYO63_001059"
     CDS             complement(4779..5066)
                     /locus_tag="VYO63_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1671995.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            5491..5811
                     /locus_tag="VYO63_001060"
     CDS             5491..5811
                     /locus_tag="VYO63_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1671996.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(5864..6436)
                     /locus_tag="VYO63_001061"
     CDS             complement(5864..6436)
                     /locus_tag="VYO63_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1671997.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            6678..7349
                     /gene="sdaAB"
                     /locus_tag="VYO63_001062"
     CDS             6678..7349
                     /gene="sdaAB"
                     /locus_tag="VYO63_001062"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1671998.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            7358..8230
                     /gene="sdaAA"
                     /locus_tag="VYO63_001063"
     CDS             7358..8230
                     /gene="sdaAA"
                     /locus_tag="VYO63_001063"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1671999.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            8252..8884
                     /locus_tag="VYO63_001064"
     CDS             8252..8884
                     /locus_tag="VYO63_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1672000.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            8934..9011
                     /locus_tag="VYO63_001065"
                     /pseudo
     CDS             8934..9011
                     /locus_tag="VYO63_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(9277..11127)
                     /locus_tag="VYO63_001066"
     CDS             complement(9277..11127)
                     /locus_tag="VYO63_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1672001.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
BASE COUNT         3393 a         2435 c         2008 g         3292 t
ORIGIN      
        1 tacaatcaag ataaaaacat gtgtaagcaa atctgctaca ctttactgga ggacgccaag
       61 aataagaaaa gctacgatag gctggctatc tgctatgtcc gtattgggat ttgtagggat
      121 gatgcgaaac ttatccaaaa agggttctcc cttctggagc taaccgagga aacttctata
      181 ctgtttcatc tcaaaaaaga agtagagatc tattaccaag cgaaggaaag ataaaaaagc
      241 cgagggattt cctcgactct ttacatatta tttgttcgtt agttcttaat accagctatt
      301 gttaagccag aagtttttag ctctgccaag ccacatagtg tgtttactac tatgtgtcca
      361 tgtcaccatg aatcgccgaa cgatttaacg agaaagtatg acttttccgt ttatcccaac
      421 tcaattatga catttttttc aaaagtcaat atatctcact ttttcaacga caagaaagag
      481 gctgataatc taccaacctc ttattctgaa cccatcactc catcactttt tagcttcatt
      541 cgctttctta gcgactgcaa tctggtattc gacttggtca ttccccttac cggtacaacc
      601 atgagcaatt gtagtcgctc ctatctgatg cgctatttca accaattttt tagaaatcag
      661 agggcggctc aaggcagata ccaagggata cttttgttca taataggcat gtgactgaag
      721 agccactagc acataatctg tagcaaattc gtccttaaca tcaatgacat aagattctac
      781 tgccccaacc ttaagagcct tatcatggat gaaatctaag tctttccctt cacccacatc
      841 catacaaact gaaacaacat catagtcttt ctttaaccag gtaatagcaa ctgatgtgtc
      901 caaaccgcca gaataggcca aaattacttt ttctttactc atgttctttt ccttctttct
      961 atttttcaat ggatgcttta aaggcatcct caatgagttt gtctaattcc ccagactcct
     1021 tcaacttttg aatggtttta tcgactgcct ctttcaattc cttgctatct tttttcatgg
     1081 ctaccgcgta ggaatcatct tgctcttttt caaaattgag gtctgcgatt gctaaatcag
     1141 gattattttc cacaaatccc ttggaaacag gttcttcaaa gataacggca tccacttgtc
     1201 ctgattttaa atctgtgatt aaattcccat ttttaggcag agatacgagg gaagaatttt
     1261 gtagcaaatc tttcgccatc gtctcttgaa tcgaaccttt ctgcgctcca acctttttct
     1321 gcgccaagtc gtttacagac tgataagtag tcaagtcaga ttttttgaca atgagtttat
     1381 tttttgcagt atagtaggga atggaaaagt caaacacctt gctccgttca tctgtcttag
     1441 aaacacctga tatggcaagg tctgcttttc ctgattgaac actagccagt acattatcaa
     1501 aactcatggg agatagttcc aattcgacac ctagttctgt tgcgatagcc ttggctaatt
     1561 cgatatctga acccacaatc tgatttttcc catcaaccac tttttgatat tcaaatggag
     1621 caaaatctgg attgagggct acaaccaatt ttcctttgga cttgatggct tcaattccag
     1681 ccgattgatt gttactacaa gcaaatagta gggggagcat cactaaacca aacatcgtca
     1741 tcaacacctt cttcatctta ttcataacaa agccttcttt atgtttattc ttttttattg
     1801 tataaataat actcctatac tcatatactc atcctttgtc aagcctaaat tgaaaaaatt
     1861 ttcaatttga aacaaaaaaa aacctagaaa tgctacataa aatgtcattc ctaggcgtat
     1921 ttatgctatt tttctctgga aaacatgagt attcagccag tcaaaaggag ctgaatgaac
     1981 tcattttccc tcgcccaatt caatgacacg atgacattgt tgggctacat aagcatcgtg
     2041 ggtcacaata atgactgttt tcccttctcg attcatctct aagagaaact tcaagaccaa
     2101 atctctattt tcaggatcca gcgaacctgt aggttcatcg gctaaaatca gctggctggg
     2161 ttttaagatg gctctagcaa ctgcaattcg ttgttgttcg cccccagaca actcggagac
     2221 cctttgatgc aaagtagctg acaaacctac tctctctaaa atctcttcca cctttttgag
     2281 cttgtctttc ttaggcaatt tcacatattt cagcgccagc atgagattgt actcgaccgt
     2341 ttcatcatca atcagggcaa aattttgaaa cagataagag atatgttcac ggattattgt
     2401 ttgcgactta gcagaattaa ccgctagatt tgtctgacca aaaatctcat accgtccgct
     2461 ataatcacca tctatcaaac ccaataaatt taacaaggtc gacttcccac taccactctt
     2521 accaacaata gctaccaaat ccccctgatc aatcctgaga gataagttat ccaaaatcac
     2581 ttttccccca atggttttgg taatattttt caactcaatc ataagatgcc ccctttcaat
     2641 aactctacta gacttctttt ctccatccta gaagctaagc ctagcacaaa tagtatatcc
     2701 agacatgtaa aacctgcaaa cagtagaagt ggtaagaacg catgggcaaa gaaaatcaag
     2761 actagaagag ggaaactata gcccagcaag agcagaacga ggagaggacg gtagcgatcg
     2821 accagtttcc accccataaa cttcttggta atgatatccc tgcgcttcaa taagaaagtt
     2881 gttactagta agaagtagga aatcatcatg ctaaggagac caaacaaagc aaagagtagg
     2941 ttaaaattcc gaacagcatc tctataagaa tccactttct cttgttgaat ggcttgaata
     3001 gatgaaaatt ttaaataatt tccatctgac aatttctcaa ctaactctgt aatctctttt
     3061 tgatgttgaa ccgtattttc aattttaatc ggattattta agccagttgt tgacagggag
     3121 gctttctcat cccacatcat atcagaatca ttgaccaagc taataattgg attggagaga
     3181 ttttcctttc gcttatcact atatgggaaa aatgaccaat ctccttcata ataggcaatc
     3241 tcgacatcca tctcctctat cgttcgtttt tgctgctctt catacttcat cgaatgaaag
     3301 gcaattaact tccccaagag ctgattttta tcttcttcac ctttcgtact tgctggcatc
     3361 aaaataactt ttttaatacc ggtatctggt agcttgaatc ccttgctctt tagaaaattg
     3421 cgattggcat agtaaacatc caccgtatct gttaactgat attgctgaat ctgttctgat
     3481 tggacaaaat tttttacagg aagactgcta ctctgcacat agcccgcctg cgttttttct
     3541 accaaatcct gataaaatcg atagaaataa tctgtagatt tccctgaccc tgctagctct
     3601 tcttgccaca gattatcatt gagtttgaag gtttctaagg tcaggtaatt accttgactt
     3661 acccactgtt gctgataagc aagttctttg ttttcttgtt ctaaacttct gcccacccca
     3721 atcagtaagg ccgtcagtaa aatagttgtc cctattttca tcacataatt gaagataaga
     3781 ccaaatttga aagatgaaaa acctttcagc agagagctga ttgtcatttt ttggattaag
     3841 aggtaagtca accaactgat aaagagataa agctgcaaca gcaaaaaatg agacaaccac
     3901 agcataggaa acaaatcttt tggcttataa tcaagcaaga aaaacacgcc tagattgatc
     3961 acaagagccc cacctaggag gaggtaaagg ttgcctttta caacatcagc taaaacagcc
     4021 ctatcttgaa aaccaagtaa tttttgtacc ccaactcttt tcatctccat catcggttga
     4081 tacactgtca ctaacacaag aagcaaaata gccaagacaa aaacaatggc agataaaagc
     4141 aaatctcgat ttatgacttc cactgcactt ttgtaggtcg gctctagcaa ggtagcctgg
     4201 tctatcttga aaaaatcgct ccatttctgt acaatcctat ccttgtccat ctcttgtgta
     4261 gaagttatcg tatagcgacc atttaaacta cgagatgtat ccttgatata ggtttgaaaa
     4321 gtcataagct gaataggttt ggcttttaga aaggtcggaa tcgtaccaag tttattggaa
     4381 atttctttat tactatagac tccttcacca tctgtggtaa aatcaagaga agaaatccca
     4441 aactcttggt aggggaaggt atctttatca aaaacaccag acttgaccac ctcatcacca
     4501 ctgtctgttt tgatgatgga gactttatac tcctttgata catcctcaaa aaatcgaaga
     4561 acagacgctg caggttcgtt aatatctttc aaatacaaat ccaaagaatc tacagtcttt
     4621 cccatttctt gaatccgaac cacttctcgt gtatagttta cattaaaaac aaaaaaagtg
     4681 gtacacagaa gcaggagtaa catacagaaa ttactgattt ttttcataaa agtgcctcca
     4741 ctacagtttt ccctctccaa ataggagagg gaagaatttt actcaaatcc atagaaatat
     4801 tccattcctg ttggcggaat cttggtgagg gaagctctag cccatgcctc aggttttgca
     4861 taatccttag aggttctacc cccatgtcta acagttgctg tatgatatcg agtagaatgt
     4921 aagtaatcag aatatccaaa agttcctgtc caacctactc catagttcca ttgaccaccc
     4981 tcaacccata ctgctaaagc atttgtagta gcaagtatac cacataccat taaagatgca
     5041 acaccaattt taactaattt tctcatgatt tatttctcct attatcgaga gctgttccgt
     5101 aaaaacagct atagtaattt atcaacaagt atcgtagtcc attggactca ccttccttct
     5161 atctcatttc attttttaac aacgaactta ctcttaaccg ggagaatggt gcgcttctgt
     5221 atcaggtcct tgaacaacca gagtagtatt aagcacactc taactattac tcaagaatca
     5281 gtccaataaa caatatggat agcgctttca aatttttaaa ctcctatccc atccttttat
     5341 ctatataata agcgaaaata taataactgt caagtaactg aagtgaattt tataaaaaaa
     5401 ttacaagcca aatttgtaaa gtttacacta agccgctagg gcaatcgtct atcagaatgt
     5461 ccgtttattt gtcaataatc cgagaaaatc ttgcaacgct tagaagtcta taaaaactat
     5521 caacatttat atgacttgcg aatagcaatc ctgctaaacc tttccacact ctatctatac
     5581 aatcaagata aaaacatgtg taagcaaatc tgctacactt tactggagga cgccaagaat
     5641 aagaaaagct acgataggct tgctatctgc tatgtcccta ttgggatttg tacagataat
     5701 gctaaactta tccaaaaagg tttctcactt ctggagctga ccgaggaaac ttctatgctg
     5761 tctcatctca aaaaagaagt agagacccat tatcaaccaa agaaattata aaaaaagtcg
     5821 agggagctcc tcgacctttt acatattatt tgttcgttag ttcttaatac cagccattgt
     5881 taagccagaa gtttttagca gcagtccatg aaccgtaacg tcctgcaacg taggcatctg
     5941 ctacacgttc ttggttttca gctgagtagt caccgttcag gtatgaatct gttaattggt
     6001 aacgtccgat ataacgtcca tttgtagctg tatagctacc acctgattct ttttgagcga
     6061 tccattcttt ggcttctgct tcagatccgc ttacagttga aacaactgtt tcacttacta
     6121 ctggagctga aacagtttca tcttgagcgg ctggtgccgc ataagtagct ggcgctggtg
     6181 ttgcaacagg cgctacaggg ccatcgataa ccaactcttg atcaacataa atcaaatgaa
     6241 tgttatcaat gtggttgttt tctgccaatt tttcaactgt tgtgttgtga gtttcagcga
     6301 tttctgaaag tgtatcacct tctttaacag tgtaagttga tgattcttga gcagatacaa
     6361 atgatggagc aaatactgca aacaaggcag ctactcctgc aagagttgct ttaatctttt
     6421 tagttattga tttcatattc gaaaattctc cttctttcta tagttctatc atacccttta
     6481 aatattaccg tttcttgaca cttttatgta gaaatattac aaaattattt tttatttccc
     6541 taaataaggt gttttttgtc ataaaagata ctatttttat gctatttgag gtattagaaa
     6601 ggttttcatc cacaattaaa gccaagcccc tcattctttg atataataga gataagaatt
     6661 tttataaggg gaaactgatg aaatcacttc gttttcaatc tgtctttgat atcatcggac
     6721 cagttatgat tggcccatct agtagccata ccgctggtgc tgttcgtatt gggaagattg
     6781 tctcttccat ttttgatgat actccgacag aagttgaatt ccaacttttt aactcatttg
     6841 ccaagaccta tcgtggtcac ggaacagacc tagcccttgt tgcaggtatt ttaggaatgg
     6901 atacagatga tcctgaaatt ccaaatagtc tggaaattgc ccacaagcgt ggtatcaaga
     6961 ttgtctggac cattcagaaa gacagcaatg cccctcaccc taacaccact aaaattactg
     7021 ttaaaaatgc ccacaagacc atcagcgtga ctggtatttc tatcggtgga ggaaatattc
     7081 aggtaaccga actcaatggt tttgccgtct ctctcaatat gaatacaccg actatcatca
     7141 tcgttcatca agatattcca ggtatgattg cccttgttac agaggcgctt tcccgctatg
     7201 gtatcaatat cgcccagatg aatgtcactc gtgaaaaagc tggtgaaaaa gccattatga
     7261 ttatcgaagt tgacagtcgc aactgtgatg aggctatcga agaaattcga aaaatccctc
     7321 atctccacaa tgtcaatttc tttaaatagg aggaagcatg ttttattcta tcaaagaatt
     7381 ggtcgagcaa gcagatctag actttcaagg aaatgtcgca gaactcatga ttacaacaga
     7441 gtttgaattg accggtcgcg aacgtgaaga agtcttcctt ctcatggaac gcaatctgga
     7501 agttatgaaa gcctctgtcc aacttggcct caatgaaaat aaatctcgta gtggcctgac
     7561 aggtggagat gctgccaaat tggatcacta catcgaaaac ggaaaaactc tgtcagatta
     7621 cacgattctc tctgctgccc gaaatgccat cgcagtcaat gaacacaatg ccaaaatggg
     7681 cttggtctgt gccactccaa ccgctggaag tgctggctgt ctgccatccg ttctcactgc
     7741 tgctattgaa aaattagacc tcagccacga gcaacaattg gatttcctct ttgctgctgg
     7801 tgcctttgga ctagtcatcg caaacaatgc ctccatctca ggtgctgagg gtgggtgtca
     7861 agctgaagtt ggttcagcct ctgctatgag tgctgccgcc ttgactctgg ctgcaggtgg
     7921 aacaccttat caggccagtc aagctattgc ctttgtcatt aaaaatatgc taggcctcat
     7981 ctgtgaccct gttgcaggtt tggtcgaagt tccctgtgtc aaacgtaatg ccatgggagc
     8041 tagctttgct ttcatcgcag cagacatggc cttggcaggt atcgaatcta aaatccctgt
     8101 ggatgaagtg atcgatgcca tgtaccaagt aggagcaagc atgccaactg cctttcgtga
     8161 aacagctgaa ggtggactcg ctaccacccc tactggtcgt cgcctccaaa aagaaatttt
     8221 cggagaataa gcttatcaaa taggagaaac catgacctct atcacagcga tttttttcga
     8281 tttggatgga accctcgttg atagttctat cgggattcac aatgccttta cctatacctt
     8341 taaagagctg ggggtgccta gccctgatgc caaaactatt cgtggtttta tgggaccacc
     8401 tctcgaaagt agttttgcga cctgcctgtc caaagaccaa atttctgaag ccgtgcagat
     8461 atatcgttct tactataagg caaaaggcat ctatgaagct caactctttc ctcagattat
     8521 agacttgctt gaggagttat cgagcagtta tccactctac atcaccacga caaaggatac
     8581 atctactgct caagacatgg caaaaaactt ggaaatccat catttctttg atggcattta
     8641 tggttctagc cctgaagcac cccataaggc agatgtcatt caccaagcct tgcagacaca
     8701 tcaactagca ccagaacaag ccatcatcat cggagatacc aagtttgata tgctgggagc
     8761 tcgagaaaca ggtattcaga aattggccat cacttgggga tttggagagc aagcagatct
     8821 actaaactat caacctgatt atatcgctca caaaccctta gaagttttgg cgtattttca
     8881 atagcataga ctgggtaaca attccatttc agaagaaaat gaagtaatac tcaatgaaaa
     8941 tcaaagagca aactaggaaa ctagctgcgg gctgctcaaa acactgtttt gaggttgcag
     9001 atagaactga cgaagtcaga tcaaaacact gttttaaggt tgtggatgga actgacgaag
     9061 tcagatcaaa acactgtttt gaggttgcag atagaactga cgaagtcaga tcaaaacact
     9121 gttttgaggt tgtggataga actgacgaag tcagtaacca tatacggcaa ggcgacgttg
     9181 acgtggtttg aagagatttt cgaagagtat aagtctctac ataataaaac gcatattacc
     9241 aaggttgtgt aactggatac tatgcgtttt tcttttttat tcaatgtggg ttgtttgatt
     9301 agcaaaggcg ataatagctt gcttcaactc atctccactg agagtgcgga actctccaat
     9361 cttttgattg atggattcta aaggcatgac attaacctta ccaacgaaaa tcttatccat
     9421 aacttgatta tcaacgagtt cggttgatac caagagttct tcgtagagtt tttcacctgg
     9481 gcggattcca acttcaacga ttggaatttc actttcagtg tggccactta gaagcaccat
     9541 cttcttggcc aagtcataaa tcttgactgg tttgcccata tcaaggataa agacttcccc
     9601 atctttggca taagcaccag catggataac cagacggcta gcttctggaa tggtcataaa
     9661 gtaacgggtc atgcggaagt ctgtcaccgt tacaggccca ccttcagcaa tctgacgttc
     9721 aaagactgga atgacactac cacggctacc aagaacattc ccaaaacgaa ctgcacagta
     9781 ggttgattgg ctacgttggt taaagccagt gacaatcaac tccgccacgc gcttggttgc
     9841 tcccataaca tttggtggat tgactgcctt atctgtcgaa atcataacca tcttagacac
     9901 tttagcttca tcaacagcct tagcaacatt gtaagttcca cggatattgt ttttgaaggc
     9961 ttcttttgga ttgcgctcca tcataggaac atgcttgtgg gcagccgcat gataaacaat
    10021 agcaggtttg tactgctcaa agacttgcaa caaacgatca tagtcttgaa tgtccgcaat
    10081 cacaggtaca taatcaatcc cttggaactt acgaatcaat tcatgataaa caaggtagat
    10141 tgagttttcc ccatgaccga gcaagacaat gcgttcagga ttgaagcgac taacttgacg
    10201 acagatttca gaaccgattg aacctccagc tcctgtgact aagatggtct tacctgtcag
    10261 ttctgcaccc agacgcgatt cgtcaagacg gatttcctga cgacccaaaa ggtccgtaat
    10321 atcaattttt tggaagccag tacctgcttg gtgaaggccc tgaacaacag tttcaacctt
    10381 aggcatcttg taacatttga cacccagctt attacacatc tgcaagatac gctcatattc
    10441 tgacggatcc agcgacggaa tcgcaacgat gacacgctcg atttgatggc gtttggctaa
    10501 ttcaggcaga ttgtcataag agcccaaaac aggaatacca ccaagttttt gacccttttt
    10561 cttagaatcc ttatccaaaa taccgaccag ttctaattca ctggttggat gttggtaact
    10621 atccataaag agagccccac catcaccggc accaatcaag aaggttcgac ggtgttctcc
    10681 atcaccacta ccttttttgc gtctggagta gattaactgc caagtaatcc gtggcaataa
    10741 aatcaagaag gtactcaaca agataaagag aatgatgaaa cggatggaga agagtggcaa
    10801 gaaggcataa cagatactat atgacaagac actgctagca gtcacaccaa aaaagatttt
    10861 catgaaatcc gtaatcttgc tgtaacgact aatgctcgcg ttcaaccccc aaaaaccaat
    10921 catcaattga tagaacagga aggccaaact cgtatagata atgtagtcaa caggtgctgg
    10981 attaatcagc ccataaaata aaatataaga tacaatgatg gaaaccacca tactgaaaat
    11041 atcaaatatt ccccagaaaa cctgcttttg ttgtttattt aaaatttcca ccagatcaat
    11101 cacataatct gttagttttt tattcata
//
LOCUS       DAVLYR010000052        11093 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLYR010000052 DAVLYR010000000
VERSION     DAVLYR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.052"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..463
                     /locus_tag="VYO63_001067"
     CDS             <1..463
                     /locus_tag="VYO63_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1672002.1"
                     /translation="EEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGL
                     STIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIP
                     ISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1384..2745
                     /gene="trpE"
                     /locus_tag="VYO63_001068"
     CDS             1384..2745
                     /gene="trpE"
                     /locus_tag="VYO63_001068"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1672003.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEELEKALNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            2742..3308
                     /locus_tag="VYO63_001069"
     CDS             2742..3308
                     /locus_tag="VYO63_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1672004.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQTIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            3319..4323
                     /gene="trpD"
                     /locus_tag="VYO63_001070"
     CDS             3319..4323
                     /gene="trpD"
                     /locus_tag="VYO63_001070"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1672005.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIITGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            4320..5087
                     /gene="trpC"
                     /locus_tag="VYO63_001071"
     CDS             4320..5087
                     /gene="trpC"
                     /locus_tag="VYO63_001071"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1672006.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5074..5673
                     /locus_tag="VYO63_001072"
     CDS             5074..5673
                     /locus_tag="VYO63_001072"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1672007.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     YFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            5651..6874
                     /gene="trpB"
                     /locus_tag="VYO63_001073"
     CDS             5651..6874
                     /gene="trpB"
                     /locus_tag="VYO63_001073"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1672008.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGCPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            6867..7643
                     /locus_tag="VYO63_001074"
     CDS             6867..7643
                     /locus_tag="VYO63_001074"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1672009.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(7615..7716)
                     /locus_tag="VYO63_001075"
                     /pseudo
     CDS             complement(7615..7716)
                     /locus_tag="VYO63_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            7945..8676
                     /locus_tag="VYO63_001076"
     CDS             7945..8676
                     /locus_tag="VYO63_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672010.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            8691..9842
                     /locus_tag="VYO63_001077"
     CDS             8691..9842
                     /locus_tag="VYO63_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1672011.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(9894..10553)
                     /locus_tag="VYO63_001078"
     CDS             complement(9894..10553)
                     /locus_tag="VYO63_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1672012.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACGIIFGKLLL
                     V"
     gene            10623..11069
                     /locus_tag="VYO63_001079"
     CDS             10623..11069
                     /locus_tag="VYO63_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672013.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVQKYSRLKLE"
BASE COUNT         3476 a         1982 c         2582 g         3053 t
ORIGIN      
        1 tgaagaaaaa tttaaaaata tgtggttgct tatgggagga gaatctcatg ttaataagga
       61 tcgtgaaaga ggtgtgcgtt cttttctgaa tgataaaaat tactttatgg atttaaaagt
      121 catttatggc ttatctacaa ttgattcagt ttattcttat gcaatgcagc acttgactac
      181 ccaaaattat ccggacatta tttttgtttc aggagatgaa aaggttcagg gcttgattcg
      241 tgcgtgctat gagaaaggaa ttcttattcc agatgatatt tctatcattg gatttgataa
      301 tattcctatt tctcagtatt atactccagc tttgtctaca attgctccca attatgttaa
      361 attggctaaa gaaatgatag aaggtgtttt agcaattatt aaaggggaaa gtgtcacatc
      421 tgttgaagtt tctcctaaat ttgttaggag acagagtttc tagtattgtt taattcttga
      481 aaatcctagt ggaaaagatg gagtgttcta aaataagttc tgtttagcct ccattcagtt
      541 tctattgagt tggatccaat ttctaacaag tgggacatgt tcatgttcga tctaaggaag
      601 atatggtcac attcgaatga tatcaaaaaa atcgtaaaaa agtcttgaca aagaaaaaac
      661 aaagtattaa aataagttca acaaatcaga aaagtaacta tttttgatct tcagggagcc
      721 tgtggtgatt gtgaataggt ggttggaagt agtgaaagtg ggctgatttt gaaaatgaat
      781 ttgaaacaat gaaaattcgg tgcgcacacc ttacagtgca gcttgttgtt aaacaaggca
      841 gagatgtaaa gggatagtcc ctttataatt gaggtggcac cgcgttacca acgccctcac
      901 acggaagtat attctgtgtg tgggcttttt tctatccgtc gttacctacg ataaggcgac
      961 gttgacgcgg tttgaagaga ttttcgaaga gtataaaggt gtaccgagat gaacatctct
     1021 tcatcaatgt ctctggtcgt ggaggcaagg acctagctgc gattgcagat tactagaagc
     1081 taaaaaataa tagatggaaa aattacagtc ttttctctca cagagagctt gtggttgctg
     1141 aaaacaagca gagaaagctg taaagttggg ttcatctgaa acgagagaag aaaacatcat
     1201 tctcaaggga gtgcccttta tcgcacagcc tgttacagga ggtatctgag acaggaatga
     1261 gagataggag aaatcctata attgaggtgg caccgcgaat ttcgtcctca cgcaagttat
     1321 tttgcgtggg gatttttcat acagtcgcca cgaactagaa ttagaactga aagggaaatt
     1381 acaatggaac gaatcattca tggagatgtc ttatcaccaa tcttggctta tatgcgctta
     1441 aaggggcaac ataaggttat tttagagagt attccgagag acaaggaaac ggctcgtttt
     1501 tctatcctag cctataatcc agtttttgag attaagtttg aaaatggagt tctctatcaa
     1561 aatggtcaag tgattgatcg ggatcccttg gatttccttt atgaagtgat tcataagagt
     1621 cagcaccact cagagctccc ttttggtggt ggagccattg gttttgtggg ttacgatatg
     1681 atttcgcttt atgaagaaat tggtcaaatc cctgaggata caattgggac gccagacatg
     1741 catttctttg tctatgagag ctatatggtc tttgaccaca agaaggagaa gatacatgtc
     1801 atcgaggatg ctctctatag tgagcgtagt caagaagaat tggaaaaagc cttgaaccaa
     1861 gtgcttgagg aattacgcat tcctgctcca aatgagtttg aagacttgga tctatctccg
     1921 ttagacttca aaccgcatat cgctcctcag aagtttgagg aaatggtgga aacagctcgt
     1981 gacttgattc gtaatggtga tatgttccaa tgtgtactca gccagcgttt ctcagcagaa
     2041 gttactggaa atccatttga cttctacaga aatctccgcg taactaatcc ttctaattac
     2101 ctttatttct atgattttgg ggattatcaa atcatcggag ctagtccaga aagtttggtt
     2161 tctgtcaaaa atggcatcgt gacaaccaat ccgattgcag ggacgcgacc aagaggggct
     2221 acggatgaag aagacaaggc cttggcgact gacctcctct ctgatgagaa ggaaacagca
     2281 gaacatcgaa tgttggtaga cttggggcgt aacgatattg gccgcatctc tgaaacggcc
     2341 agtgtccaag ttactaagta tatggaggtg gagctcttcc gctacgtcat gcatttgacc
     2401 agtgtggtca agggacgttt gcttgcagaa ctcactgcca tggatgcctt gaaagcaacg
     2461 cttcctgctg gaaccgtttc tggagcgcca aagattcgag cgatgagacg tatctatgaa
     2521 ctggaaacgg aaaaacgggg agtatacgct ggggcaatcg gctacttgtc tgcgacgggt
     2581 gatatggatt tggccatcgc catccgaacc atgattctca aaaatcaaag agcctatgtg
     2641 caggctgggg cagggattgt ctacgactct atcgcccaaa acgaatacca agaaaccatt
     2701 aacaaagcaa aatctatgac tagaattgga gaactaagac catgatttta ttgattgaca
     2761 actatgattc ttttacctat aacttggccc aatacattgg gaattttgca gaagttcagg
     2821 ttctgagaaa tgatgattcc aagctgtatg aagaagctga aaaagcagat ggtctggtct
     2881 tttctcctgg tcctggttgg ccagttgatg ctggaaagat ggaagacatg attcgtgatt
     2941 ttgcaggcaa gaagccgatt ctagggattt gtttgggcca ccaagccatt gcagaagtct
     3001 ttggtggtaa gctaggtttg gctccaaaag tcatgcatgg gaaacagagc aatatcaact
     3061 ttgaagcgcc atctgttttg tatcaaggta ttgaggatgg acgtgcggtc atgcgttatc
     3121 acagtatttt gattgaggaa atgccagaag actttgaagt gacagctcgt tcgactgatg
     3181 accaaaccat catggggatt caacataaaa acctaccgat ttatggcttc cagtaccatc
     3241 cagagagcat tggaacgcca gacggcttgt cttctattcg gaattttatc gaggaggttg
     3301 taaagtgagg aaactaggat gaaagagatt attgaaaaac tagcaaaatt tgaaaattta
     3361 tcaggtgtgg aaatgacgga tgtcattgag cgtatcataa ctgggcgtgt aactgaagcg
     3421 cagattgctt ctctcctctt agctcttaag atgaaggggg aaacacctga agaacgcaca
     3481 gccattgccc aagtcatgag aggacatgcc cagcatattc caactgagat tcatgatgcc
     3541 atggacaatt gtggtacagg tggggacaag tctttcagtt ttaatatttc cacaactgca
     3601 gcctttgtct tggctggtgg cggtattcac atggccaagc atggtaaccg ctcgatttct
     3661 tctaaatctg gttccgcaga tgtcctcgaa gccttgggaa tcaatcttga cctcaaacca
     3721 gctgaactag gtaaggtctt tgataaaact ggcatcgtct ttctcttcgc taaaaatatg
     3781 cacccagcta tgaaatacat catgccagct cgtttggaat tgggaattcc aacgattatg
     3841 aacttgactg gtccactgat tcatccaatg gctttagaaa cacagcttct tggaattagt
     3901 cgtccagaac tcctagaaag tacagctcag gttttgaaaa atatgggtcg caaacgtgcc
     3961 atcgtggttg ctggaccaga agggttggat gaagctggct tgaacggaac aaccaagatt
     4021 gcacttcttg aaaatggcga aatcagcttg tcaagcttta ctccagagga tttgggaatg
     4081 gaaggctatg ctatggaaga tattcgtgga gggaatgctc aggaaaatgc agaaattttg
     4141 cttagcgttc tgaaaaacga agcaagtcca ttcttggaaa cgacagtctt gaatgctggt
     4201 cttggtttct atgctaatgg taagattgat agcatcaagg aaggagttgc cttggcccgt
     4261 caagtgattg ctagaggcaa ggcccttgaa aaactcagac tgttacagga gtaccaaaaa
     4321 tgagtcagga atttttagca cgaatcttag agcagaaggc gcgtgaggtg gagcagatga
     4381 agctggagca aatccagcct ctgcgccaga cctatcgctt ggcagaattt ttgaagaatc
     4441 atcaggactg cttgcaggta atcgctgagg tcaagaaagc tagccctagt ttgggagata
     4501 tcaatctcga tgtggatatt gtgcaacagg cccagactta tgaagaaaac ggagcagtga
     4561 tgatttcggt gttgacagat gaggttttct ttaaagggca tttggattat ctacgggaaa
     4621 tttccagtca ggtagagatt ccgacgctca acaaagactt tatcatagat gaaaagcaaa
     4681 tcatccgcgc tcgcaatgca ggtgcgacag ttatcttgct tattgtggca gccttgtccg
     4741 aagaacgcct caaggaactg tatgactacg cgacagagct tggtctggaa gtcttagtgg
     4801 agactcacaa tctagctgaa ctagaggtag cccacagact tggtgctgag attatcgggg
     4861 tcaacaaccg caacttgact acctttgagg tcgacttgca gaccagtgta gatttagccc
     4921 cttactttga ggaaggtcgc tattacattt ctgaatctgc cattttcaca gggcaggatg
     4981 cggaacgact agccccatac tttaacggaa ttttggtagg gacagctctt atgcaggcag
     5041 aaaatgtggc ccagagaatc aaggagttgc agattgacaa aggttaaaat ttgtggacta
     5101 tcgaccaaag aagcggtgga aacagccgtt tcagcaggag ccgactatat cggttttgtc
     5161 tttgcaccta gtaaaagaca ggtgacttta gaagaggcag ctgagttggc aaagcttatt
     5221 cctgcagatg tgaaaaaggt tggagtattt gtttcaccaa gtcgggtaga actgctggaa
     5281 gcgattgaca aagttggctt ggacttggtt caagttcacg gtcaggtggc agatgattta
     5341 tttgagaatt tgccttgtgc cagtattcag gctgtgcagg tagatggaaa tgggcatgtc
     5401 cctaattctc aggcagatta tctactcttt gatgcccctg tggcaggaag tggccagccc
     5461 tttgattggg gtcaactgga tacgactgga ctagcacagc ccttctttat cgcaggtggc
     5521 cttaatgaag ataatgtagt aaaagcaatt caatatttta ctccctatgc agtagatgta
     5581 tcgagcggag tggagacaga tggacaaaaa gatcatgaaa agattagaag atttatagag
     5641 agggtaaagc atggcatatc aggaaccaaa taaagatgga ttttacggaa aattcggcgg
     5701 acgttttgtc ccagaaacat tgatgacagc agttttggag ttggagaagg cctaccgtga
     5761 aagtcaggca gacccaagtt tccaagagga attaaaccag cttttgcgcc agtacgtagg
     5821 acgtgaaact cctctttatt acgcaaaaaa cttgacccag catatcggcg gagccaagat
     5881 ttatctcaaa cgggaagacc ttaaccatac aggagcccac aagattaaca atgccttagg
     5941 acaagtttgg cttgccaaac gcatgggtaa aaagaaaatt atcgcagaaa cgggtgctgg
     6001 tcagcacggt gtggcaactg caactgctgc ggccctcttt aacatggaat gtaccatcta
     6061 catgggtgag gaagatgtca aacgccaagc cctcaatgtc ttccgaatgg agcttttggg
     6121 agctaaggtc gaggccgtga cagatggttc gcgcgtgctc aaggatgcgg ttaatgcagc
     6181 ccttcgttca tgggtggcta atatcgatga tacccactat attcttggtt ctgccttggg
     6241 gcctcatcca tttccagaaa ttgttcgtga cttccaaagt gtcattggtc gagaggctaa
     6301 acaacagtac cgtgacttga caggacaaaa tctgccagat gccctagtag cctgtgttgg
     6361 tggtgggtcg aatgccatcg gtctcttcca tccctttgta gaagatgagt cagtagccat
     6421 gtatggagct gaagcggctg gacttggtgt ggatacggag caccacgcag ctaccttgac
     6481 caagggttgt ccaggtgtcc ttcacggttc cctcatggat gtgctccaag atgcccatgg
     6541 tcaaattctt gaagccttct ctatctcagc aggtttggac tatcctggta tcggtccaga
     6601 gcattctcac taccacgata ttaaacgtgc cagctatgtc cctgtgacag acgaagaagc
     6661 tttggaagga ttccaactct tgtctcgtgt ggaagggatt atcccagctt tggaatctag
     6721 ccatgctatc gcctttgcgg tgaaattggc caaagaactt ggaccagaaa agtctatgat
     6781 tgtctgccta tcaggtcgtg gggacaagga tgtggttcaa gtcaaagacc gcttggaagc
     6841 agatgcagca aagaagggag aagctcatgc ctaagacact aacaaaaaaa ttaaatgcta
     6901 ttaaagcagc tggaaaagga atttttgttc cctatatcat ggctggagac cacgagaaag
     6961 gtttggatgg actaggagaa acaatccact ttctagaaga tttgggtgtc tctgcaattg
     7021 aagtgggtat tcccttttca gaccctgttg cagatggacc tgttatcgaa gaagctggct
     7081 tgcgcagttt agcccacggg acctctaccc aggctttagt tgaaaccttg aaaaccattg
     7141 aaacagagat tccactggtc atcatgacct acttcaaccc cctctttcag tacggtgtgg
     7201 agaactttgt caaagatctg gctgatacag ctgtgaaggg cttgattatc ccagacttgc
     7261 ctcatgaaca tgccaacttt gtagaaccat ttttggcaga tacagatatc gccttgattc
     7321 ctttagtaag cttgaccaca ggaattgagc gccagaaaga gttgattgaa ggcgcggagg
     7381 gattcgtcta tgccgttgcc atcaatgggg tgacagggaa atcaggcaat taccgtgcag
     7441 atttggacaa gcacttggcg caacttcatc aagtggccga catcccagtc ttgacaggtt
     7501 ttggtgtatc tagtcaagcc gatctggaac gcttcaatgc ggtgtcagat ggtgttatcg
     7561 tcggttcaaa aatagtaaaa gctctccacc aaggagagcc gattcaggat tttatcagac
     7621 aagcagtagc ttaccaaaaa taatcaaaca agtagcaagt aagaggaaag gcacgaaagg
     7681 aagtctttcc tttttctttt gcaggagaaa ggtcagtttt atagtgaatt gaatctggaa
     7741 tattacacta cggttcctaa aatattttca gaaagcgatt tgaatttccc aataaatttg
     7801 ttcagttttt aattgatttt actatagaag atataatttt ctaaattact ctgacacaga
     7861 actttaagaa aaagataatt attgtagtaa tttctttaag aaaatgatat aattcattaa
     7921 aaagtatttg gaggaggaag aattatgttg aatcaggatc tctttgattc gcttgaggca
     7981 caaaaaattg tagatacttt gatgaaaggg caaaaagatt atgtagatga acgtctagaa
     8041 aaaagagaga caatgatagt gtcgaatggt tatgcatgga cgcgacctaa tcatattgat
     8101 actgcatttg catcagcaga tttgtttgag tataaattac aattagcagg acagacttgg
     8161 ggatatttag aatttgaaac aaatacagaa aaatatggga aagtattgtt aattataaag
     8221 ggtaagaagc gacttacgaa ccaatttcct ttggtacaaa aaaataagag tggctactta
     8281 tttgaatacg ctcagatgaa tacactttat cttaatcaac attcttccta caaaaatgat
     8341 gaagatagtc attcctttcc aattcagatg gagttagttt ctgatgaaat gattcaagaa
     8401 attgaacaag ctactaaaaa ttcgaatatc gaaaaattta tgattttaac ttatgaggcg
     8461 gactcagaaa acaatattat atctgtagat gttgttatgc ctgatgcacg aactggtcag
     8521 ctacacttga ttcaagattt gtctgagtat attcaatcaa gttcgtacca tttcgaagaa
     8581 gctaaatatc aagatattcc taatttttca gaattatcgg aaacagaaga ttttgaaatt
     8641 attccaagaa tagaaaaaca agaaggtcaa aagtagtaag gagtatataa atgtttaatg
     8701 gtcgggtatt gaaagaatta cggctgttaa atggtttaag tagagcagaa ttagctcaga
     8761 gaattaattt aacggaacaa gccatttggc agtttgagtc caacgaaacg aaacctaaat
     8821 tatcaaccaa aatgcatttg gccaaccaat ttcatgttga tttaacttat tttgaacagg
     8881 aagaagagag cattcgattt gattcttctg taattgcctt tagaaatgca gacctagcaa
     8941 cacggaaaac aatagatatt caaactatgt atttacataa ggtagatagt ttgattgatt
     9001 attttgaaag ttttgtaatt atacctaata ttataattca tgacctaagt aatgtagtga
     9061 gtgaatctta tcataaggga gaatccattg aggaattggc tctttatgcc agggaaaaat
     9121 taggtatttc aaaagataat catgatttgc tttataaatt agaacgttca ggtatctata
     9181 tcgtggaacg attaattaat ggccaagctg atgcttatag cgcatggtca aaattgggaa
     9241 gaccttatat tgtgttggga acgaataaat catctgtacg tcgaaatttt gacttagctc
     9301 atgagctagg acatattctt ttacataaat ataaagatat gaatgaagat ggcaatcgtt
     9361 tggagcaaga agcaaattat tttgcatcat gttttttatt gccaaaagaa gagtttttag
     9421 tcaaatttga agagagggtt ggcaagcgtg tcagcaatcc tgatagttat attttattga
     9481 agtcggattt gaatgtttcg atacaggctt tagagtatcg agcttttaag ttaggattat
     9541 tgactccaaa gcaacattct tacttttatc gtcaaattgc gcaaaaaggt tacaaaatga
     9601 ttgaaccttt ggatgatcaa atttttgtta aaaaaccaag caaagtaaag agtattctgg
     9661 acgtcgtttt gagtaatcat ctagtcagtc tagcgactat aatgtctaaa caaagtattc
     9721 gtttacagtt tataagcgaa atattttcag tcgaaatgaa attttttgat cagtatcaag
     9781 aagatagaag aacagatcga tttgataaca tcatcccttt gtacaaaaga aataatttat
     9841 aatttaactg tgaaataatt tcatgaagga catatggcag aaattggatt ttatcagaca
     9901 agcagtagct taccaaaaat aatcccacaa gcagcaagta agaggaaagg cacgaaagga
     9961 agtctctcct ttttcttttg caggagaaag gccaggatac ccgtcgcaga agcaaactga
    10021 atcaagatca gtaactccgt tacgctaaag acgagagcac aagaagctaa aaagaggaaa
    10081 tcccctgcgc ccatgcggat atcgataaaa tgagccaaaa ttccaagagc aaggaaggag
    10141 accatgacca gattccagcc agaggaagct attaggatta ggtggaaagt catccagacc
    10201 agtaagggat attcctgatg gtgaaagtcg tagataccca aggtcaaacc agcggtgatt
    10261 aggacgactt gccccaagga aagcaattcc caagagtaaa gcagaaagag gagtcctaag
    10321 actaattcaa agagggcata ccagacagga tagcgaactt tgcagtagcg acagcgaaag
    10381 cgattgaaga cctgtgagag aatcggaatc aaatctaagg gacgcaagcg agtctgacag
    10441 gaatcgcagt gactggctga actgataatg gattgctctg gaaaacggtc aatgaccaaa
    10501 ccaagaaagg aagcgagaat gctcccgacg agaaaaaaat aaaaatcaat catacttatc
    10561 tattcgtaaa aaatgggaga aatagtataa tggagaaaca tagataagat ggtgaggaaa
    10621 agatgacaat tcgttttgaa gaaaaggtga gtatagaaaa cgctcaattt gtatgccagt
    10681 ggtccaactc cctaggcaaa tcctttcaag aacaatggat gggaccaagg attccttttc
    10741 tactgaccct tcaagccttg gaaggagtct tttcaatctt tgatgaacaa gagtttgtgg
    10801 gacttatcca gaaaatcagg ctagaagaca gcaatcttca tatcgggaga ttttttatca
    10861 acccccagaa acaggagcaa gacttaggta gccaggcttt aaggaaattt gttagtttgg
    10921 cctttgaaaa tgaagatata gatagtattt ctctaaatgt cttcgaggca aatcaaagag
    10981 ctcagaatct ttaccaaaaa gaaggatttg aaatcgttca aatggttgaa gcacctgtac
    11041 aaaaatatag tagattgaaa ctagaatagt acacctctac ttctaaaaca ttg
//
LOCUS       DAVLYR010000053        11050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLYR010000053 DAVLYR010000000
VERSION     DAVLYR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.053"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            11..733
                     /locus_tag="VYO63_001080"
     CDS             11..733
                     /locus_tag="VYO63_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1672014.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VYO63_001081"
     CDS             730..1578
                     /locus_tag="VYO63_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1672015.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VYO63_001082"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VYO63_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1672016.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VYO63_001083"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VYO63_001083"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1672017.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VYO63_001084"
     CDS             complement(3249..5957)
                     /locus_tag="VYO63_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1672018.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQCSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATDQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VYO63_001085"
     CDS             complement(5959..6660)
                     /locus_tag="VYO63_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672019.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDVLNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9320)
                     /locus_tag="VYO63_001086"
                     /pseudo
     CDS             complement(6910..9320)
                     /locus_tag="VYO63_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
     gene            complement(9373..10065)
                     /locus_tag="VYO63_001087"
     CDS             complement(9373..10065)
                     /locus_tag="VYO63_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1672020.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10600..10962)
                     /locus_tag="VYO63_001088"
     CDS             complement(10600..10962)
                     /locus_tag="VYO63_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672021.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
BASE COUNT         3226 a         2530 c         2062 g         3232 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gttgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ccgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gcatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcccac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggatagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccaag aaccttggta
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 acagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ctcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgatcggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcggcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaaccagc tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttgtcttc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag ttgcagacgt
     5221 accttgccat tatctgataa agtttgttca aggtcttcct gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ctgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gcattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgcctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatact agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat atgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tatcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtaggtt catcacaaag
     6181 aagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tatttagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aagacatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctga tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagcactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct gcaacctcaa aacactgttt tgagcaacct gcggctagct tccttgtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttgaaaactg cttatattct taaatcctta ttaaaaggca gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatgtc tttaactgct ctgccaataa
     7321 tttatgggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggctagca cgcgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 atgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatt agagattggt cataagtatt gccaatcgaa atcggcccat gttctgggga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctct ctgagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttttcctcac ctttttgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgacc ggaaagaccg cctctaggag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctctcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat atgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agacttttcc tgttcatact ttccattaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttcca acccttcctt agtaaaacat tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcgc
     8881 aagtgcctta ctaatattca gctgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaca tactgatcct gaagatttcc accctgataa
     9121 tctgaactat caggaagtgg acctccagac cagagacttt tttcattaaa ttgaatccgt
     9181 tcagccccta taaggccaaa tacttttgct cctaaagaac cattgcctat cggtaaagct
     9241 tcttcttccc aacccatgta agtggttgaa gctggttgct tgtaggccag tacataatct
     9301 tgttttttat tccttatcat acttccatac taacataaaa agcctagaaa tcaattctag
     9361 acttacaatt ttttattttc caaggtagta ttcagaaacg acgttcaatt tttcgtcgaa
     9421 ttcgaatacc aatggtggga agttagggat ttccacgtcc atgatctcgt catctgacaa
     9481 acctttgatg tgttttacaa gggcacggat tgagttaccg tgagctccta cgaatacgtt
     9541 tttaccatct ttaagagctg gagcgatttt atcttcccag aatggaagag cacgttccaa
     9601 agtcactttc aagttttcag catctgggat aactgagtcg tcaagtgaag cgtaacgacg
     9661 gtctgtgtga gctgagtgct catcatcacg gtccatgttt ggaggcaata catcgtatga
     9721 acgacgccag atgtgaactt gctcatcacc aaattgttca gcagcttcag ctttattttt
     9781 accagtcaaa ccaccgtagt gacgttcgtt caagcgccat gatttttcaa ctggaaccca
     9841 caattggtca gaagcttcaa gagccaagtt agttgttttg atagcacgtt tcaatactga
     9901 agtgtaagct tggtcaaatt tgataccagc ttctttgatc aatttaccag cgtcaatcgc
     9961 ttgttgtgta cctttttcag acaaatcaac atcagcccaa ccagtgaaaa ggttagcttt
    10021 gttccattca gactcaccgt ggcgagcaaa aaccaatttt accattagat ggattctcct
    10081 ttaaattttc cgaggtttcc cctcgtttat ctattctatt ttacacaatt tttcacaaaa
    10141 aagctagtca aaaccaaaaa ggaaaggact gtttgacaat gtttggcaat cacattgttt
    10201 ttggttgttt ctattctatc ttcattattt aacctttttt tctgcgcatc gaataatttt
    10261 tgagttgact cttttagttt agtaaacgag tcattatttt ccatagctgt gcctttgaaa
    10321 ccttcctcag ttttagtaaa ataatcatca aaaatatttt tataaagatc tatataatct
    10381 gcataagtgc gtattgattc aatcgcatcg tctgttacaa ccggtgtatc gaaagtgatt
    10441 gtttcttttg aactattgga tgttgctact gtcttctcat cattacttgt tgaagtattc
    10501 tttgaacttt atgttccaca tgcaactaga agagaaagag ataatagagc tactcctata
    10561 ccgataaact tttttgttct tttcatgcta aaataccttt tattcttctg atttgaattt
    10621 cttcaatgtt gcaaggaata gagatcctcc gataatagca agaatccccc caacccatcc
    10681 taagaatgga atgagactaa cttcaccaga aacaatcatt agtacagacg gagctgcacc
    10741 tacacgctta tctcctttat agtaaacaat agcaatgata ccaagaacaa gatttgcaat
    10801 cttcaaggca ttcaagagta aggcaacacc tgaaaaagcc cctgcagtag ctcctccatt
    10861 aagagttgta gcagctgtgt ttaccgctgt tcccaacaag acaaaaggac aaataagaag
    10921 taaaattcct cccacaagac cgacgatacc acttgatttc ataataaaat ctcctttttt
    10981 attttttttc aaataaaatt gtatcatatt tgctttttaa aaaaaaagta ttttctgata
    11041 ttataaaata
//
LOCUS       DAVLYR010000054        10951 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLYR010000054 DAVLYR010000000
VERSION     DAVLYR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.054"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            55..738
                     /locus_tag="VYO63_001089"
     CDS             55..738
                     /locus_tag="VYO63_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1672022.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            738..1472
                     /locus_tag="VYO63_001090"
     CDS             738..1472
                     /locus_tag="VYO63_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1672023.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESRKVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            1626..2483
                     /locus_tag="VYO63_001091"
     CDS             1626..2483
                     /locus_tag="VYO63_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1672024.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            2625..3497
                     /locus_tag="VYO63_001092"
     CDS             2625..3497
                     /locus_tag="VYO63_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1672025.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            3779..4462
                     /gene="rpiA"
                     /locus_tag="VYO63_001093"
     CDS             3779..4462
                     /gene="rpiA"
                     /locus_tag="VYO63_001093"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1672026.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            4476..5687
                     /locus_tag="VYO63_001094"
     CDS             4476..5687
                     /locus_tag="VYO63_001094"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1672027.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            5689..6246
                     /locus_tag="VYO63_001095"
                     /pseudo
     CDS             5689..6246
                     /locus_tag="VYO63_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            6246..7055
                     /locus_tag="VYO63_001096"
     CDS             6246..7055
                     /locus_tag="VYO63_001096"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1672028.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            7531..7926
                     /locus_tag="VYO63_001097"
     CDS             7531..7926
                     /locus_tag="VYO63_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV1672029.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEIIEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAAVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            7936..8511
                     /locus_tag="VYO63_001098"
     CDS             7936..8511
                     /locus_tag="VYO63_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1672030.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            8536..9048
                     /locus_tag="VYO63_001099"
     CDS             8536..9048
                     /locus_tag="VYO63_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1672031.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            9706..10416
                     /gene="deoD"
                     /locus_tag="VYO63_001100"
     CDS             9706..10416
                     /gene="deoD"
                     /locus_tag="VYO63_001100"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1672032.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(10439..10951)
                     /locus_tag="VYO63_001101"
     CDS             complement(10439..10951)
                     /locus_tag="VYO63_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1672033.1"
                     /translation="MIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSDHEWM
                     LTDIKNFLTKHKAWVIDGNYSWCCYQERMQEADQIIFLNFSPLTCLFRTFKRYLKYRG
                     KVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRELDQF
                     LDKKRKSYNS"
BASE COUNT         3298 a         1942 c         2529 g         3182 t
ORIGIN      
        1 aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt gactatgaat
       61 ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt gacgattctt
      121 atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc ttttgggcaa
      181 cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat tttccgtggg
      241 acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa tgctccgact
      301 attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat tttgattatc
      361 tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa tgcggttcca
      421 aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ctaaaaatgc gatgcgttat
      481 gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga atttatcacc
      541 attatcaagg acagctccct cttatcagct attggggtca tggagttgtg gaatggggct
      601 acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc agcattttac
      661 tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa acatatggga
      721 caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta cataaatctt
      781 ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga gaagttgtcg
      841 ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg aatttgttgg
      901 aggaagcaac caaggggaag gttatctttg agggagtcga tattacggac aagaagaatg
      961 acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac ctctttccta
     1021 atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt gaaagcagga
     1081 aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca gataaggcag
     1141 acgcttatcc acagagtctg tcgggtgggc aacaacaacg gattgccatc gcgcgtgggt
     1201 tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg gatcctgaaa
     1261 tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg actatggtta
     1321 tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc tttatggcag
     1381 acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc caagaacaac
     1441 ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa gacaagatta
     1501 gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa cctaggaaag
     1561 aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa gaaaagagaa
     1621 gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag gggcagttgg
     1681 ctgaaaagac tgcaaaatta aaggaagaaa cagctctagt gcctggtttg gtagtgattt
     1741 tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg tcagctcttg
     1801 cggctggttc ccgtagcgaa gtagtgcgag ttccagagac cattactcaa gaggaattgt
     1861 tagacctgat tgctaaatat aatcaggatc cagcttggca tgggattttg gtccagttgc
     1921 cattaccaaa acatatcgat gaagaggcgg ttttattagc cattgaccca gaaaaggatg
     1981 tggatggttt ccatccctta aacatgggac gtctttggtc tggacatcca gtcatgattc
     2041 cctcgacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgac ttggaaggta
     2101 aaaatgcggt cgtcatcggt cgttcaaata tcgttggaaa acctatggct cagcttcttt
     2161 tggctaaaaa tgcgacagta accttgaccc actcacgaac tcataatctt gccaaggtgg
     2221 ctgctaaagc agatattctt gtagtcgcaa tcggccgcgc caagtttgtg actgctgact
     2281 ttgtcaaacc aggtgcggtt gtcattgacg ttgggatgaa ccgagatgaa aatgggaagc
     2341 tctgtggaga tgttgattat gaggcagttg caccacttgc tagctatatc acgccagtcc
     2401 ctggaggtgt cggtcctatg accattacca tgctgatgga gcaaacttat caagcagcac
     2461 ttcggacatt ggatagaaaa taagagaaac attcgattaa tagagtgtat tttctatagc
     2521 tatatctaaa atggcagaaa tgaatattaa attttagata taagattaca aaaggaggtc
     2581 tgcgtctcct ttttattgta taataaaagt gagaggaaaa aaggatgaaa gtgattaatc
     2641 aaaccttatt agaaaaagtt attattgaac gttctcgttc aagtcataaa ggagattatg
     2701 gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc atggctgctt
     2761 tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg gaaaatatcc
     2821 cggctctgca cagccattta cctgagccta tggccttttc tcttcaagac cagcaattgt
     2881 taaaagagca attggagaag gcagaagttg tcttgttggg gcctggttta cgagacgatg
     2941 cttctggaga aaatctagta aaacaggtct ttgttaattt aagccaaaat cagattttga
     3001 ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt ccctcaagcc
     3061 agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt acgattgaaa
     3121 agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga acaattttgg
     3181 tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat taccagttac
     3241 aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct gggatgatcg
     3301 caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca gtagcgaccc
     3361 atcttcattc agccatagcc caagaactat ctcaggaaaa ttatgtggtc ttaccgacgg
     3421 aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagag agggtaagca
     3481 aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat ttatgattct
     3541 tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt tgtttttgta
     3601 actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta ttcgtttggt
     3661 aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt gatgttttta
     3721 cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag gtaaatcggt
     3781 ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg atggaatggt
     3841 cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg gtcgtcgtat
     3901 caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca ctaaacaggc
     3961 agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg atgtgacggt
     4021 tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg gtggtgccct
     4081 tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg tggatgaaag
     4141 caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc agtatggtgc
     4201 agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc gtgaaaaaga
     4261 cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct tggatgtcat
     4321 tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg tggagcatgg
     4381 tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag ttcagatttc
     4441 aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat cgtattcatt
     4501 tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac tttgtcaatg
     4561 caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca gttggtttga
     4621 atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact cctcttaaga
     4681 ctgtagcagc tgaaatcaat ccaactggat atgcaacaaa attagaggaa gtatctcttg
     4741 gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact gagcctttcg
     4801 atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa ttctcaggac
     4861 gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc gatgattttg
     4921 gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac cctgttttgc
     4981 agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc tgtgaatacg
     5041 ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct cgcccttatg
     5101 taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct gtatctccat
     5161 tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat gctgtgggta
     5221 aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac aacaagtcaa
     5281 atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt gaaaaaggat
     5341 tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt aatgctcacg
     5401 gttaccgtga ttgtttgcat gagtttgatg aacgcttacc tgaaattatc gcagctatga
     5461 gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg tatgcaggga
     5521 cggatcacac tcgggaatat attccattgt tggcatatag ccctgccttt aaaggaaatg
     5581 gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat aactttggtg
     5641 tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat gacgcgctat
     5701 gctttgctgg tgagaggtat caatgttggt agtaagaata aggtcgttat ggcggagctt
     5761 cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa cagtggcaat
     5821 attttcttta cttcgataga ttccaaagcc caattggttt aaaagctaga gactttcttt
     5881 gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga ctttgaggcg
     5941 gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga ttttctcttt
     6001 tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt agagctgaaa
     6061 gatgaagtgc tttattttag aaaacttggg attttctggg ggaaattttc tgaagaatcc
     6121 tattctaaga ctgcctatca taagtacttg ctgaaggtgc ctttctaccg ccacattact
     6181 attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaataa taaaggagac
     6241 acataatgac atttttaaac aaaatccatg aaactgctac tttcctgaaa gaaaagggaa
     6301 ttgcagcccc tgagttcggt ctaatccttg ggtcaggact tggagaattg gcagaagaaa
     6361 tcgaaaatcc agttgtagta gactatgctg agattccaaa ctggggccgt tcaacagtag
     6421 tcggtcatgc tggtaaattg gtatatggtg aactggcagg tcgcaaggtc ttggctcttc
     6481 aagggcgttt ccatttctat gaagggaatc ctctggaagt ggtgactttc ccagttcgtg
     6541 tgatgaaagt tcttggatgt gaaggtgtta ttgtaaccaa tgcagctggc ggtatcggat
     6601 ttggtcctgg taccttgatg gctatctcag accatatcaa catgacgggg caaaatccat
     6661 tgatgggtga aaacttggat gactttggcc cacgtttccc agatatgtct agggcctaca
     6721 caccagaata ccgtgccact gcccatgaag tggctaaaaa acttaatatc aagcttgatg
     6781 aaggtgtcta tatcggagtt actggtccga cttatgaaac accagcagaa attcgttcct
     6841 ataagacact gggagcagat gcagttggta tgtctacggt tcctgaagtt atcgtggcgg
     6901 cccactctgg cttgaaagtt ctgggaattt catgtatcac taactttgcg gccggtttcc
     6961 aagaagaact caatcacgaa gaagttgtag aagtgactga acgtgttaaa ggtgatttca
     7021 aaggcttgct taaagcgatt cttgctgaat tgtaagaaaa aagatttaaa agggggagtg
     7081 cctctgtttt ttcaggattg actgcctatc cggattaaag aagaaacaga ggaatactat
     7141 gagcttcttc ctgctcttat aactgaaaga agcggaagaa taggtgtgtc tgatctgata
     7201 gccagcattg tgaaagacaa gattctagga tactagcatt agcttcctag ccaagtagac
     7261 tagtatgata aggagagatg agaatgaatt gactttctga atttctcagt cttatcatat
     7321 atagcacaat gagatttcgc ttgagtctgc ttgtaaataa acgaaaagaa agataagaaa
     7381 taatgaaaat tggtcaacga attatgcgct ttggcataaa aaattaagta tcggagttgt
     7441 atctgttgta gtcggctttg atttctagct ccagctggaa tttcagccaa tgaagtaaag
     7501 caagatgtaa catctgaagt ggtaataggt gtgctagatt ctaaggagga attgaaagag
     7561 tcagaaaatg atgctccaaa actagaaact cctcttagag aggagccaag actagctcct
     7621 caaacgcttc cggaagcaag tgaagttctt gaaaacaaaa gggaagagtc aaaagtagag
     7681 ataatagagc cagctcaagc ggatgatatc cgcaaggttg ttggggaatt agccaaggat
     7741 ataagtatta ctaagttgta tatgacaggt cattctcttg gatgttacct agctcagatt
     7801 gcagcggttg aagcttacca aaaatatcct gatttttata accatgtatt gaggaaagtg
     7861 acaactttca gtgctcctaa agtgattact tccagaactg tttgggatgc taagaatggt
     7921 ttctgagatg ttggtttgga aagtcgtaaa ttagctgtta gtggaaaaat taagcattat
     7981 gtggttgata atgacaatgt tgtgactccc ttgattcata ataatcgtga tattgttaca
     8041 tttacaggta attcacgctt taaacaccgt tctcgtggct attttgaaag tccaatgaat
     8101 gatattccta actttaatat tggtaaacaa gctaccttgg ataaacatgg ttatcgtgat
     8161 ccgaaattgg ataaagtgcg attctttaag aaacaggctc tacctcaatc ttctagtcaa
     8221 ccaagcgctg aaccaatgga aaatattgcc ttaggaaaac aggttactca aagttcgaca
     8281 gctttcggag gagatgctag aagagctgtg gatggcaaag tcgatggtaa ctatggtcac
     8341 aattctgtca ctcatacaaa cttccaatct aagccttggt ggcaagtaga tttggctaaa
     8401 gaagaaacca ttcgccaaat caatatttac aaccgaacag acactgccca ggatagattg
     8461 gcaaactttg atgtcattct tttagacagt tctggtaaag aaattgagtg aaaacgtata
     8521 atatctccta aagatgtgtc agcacaaatt acgattaacc ataaaaaagc gcgctatgtt
     8581 cggattgggc tagaaggcta taatgccctc agtcttgcag aagttgaagt tttccgcttt
     8641 atagctacga atgctgaaac ggcgacacaa gtttctaagc cagttcaacc aatcagtcag
     8701 actcctgtga aagataaaac attgacaatt caacacagtg gagcttacat tgcccgctac
     8761 tccataactt gggaagaagt tacagtagat aaagatggaa accaagttgt tcgtagtcat
     8821 tcttgggaag gaaacggtcg caaccagact gcaggttttg tcctcaacct cccaatcaaa
     8881 gaaaatatga gaaatctgcg agttaagatt gagaaaaaga cgggcctact atggaataga
     8941 tggcaaacaa tctatgaaaa cagaccaatt ttagctcaac cccaccgtaa aattacccat
     9001 tggggtacga cattgaattc caaggtgagt gacgatgatg tcttgtaatc tgatggtaca
     9061 atgacagtta gtttgtctag tttataagaa agtactacct gagcttgaat agaactcagg
     9121 tagctctctg tgaaagaaca aaattaatac tcaatgaaaa tcaaagagca aactaagaaa
     9181 ctagccgcag gttgctcaaa gcactgcttt gaggttgtag ataagactga cgaagtcagt
     9241 cacatatata atccaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtataaac
     9301 agaaaggtag agcgcgtgtt ctaatttgaa cacgagtaga aaacttttct aaaaataaaa
     9361 acgaaaggat gggtaaactg tattcgctga actgaatacg ggcgactctc ctctaaatca
     9421 aaattaagaa agaaaggaat tgaccccacc ctaaaagcag tgggaaaaag atagttggtc
     9481 tagcgagcat cgctcactgc gcccaactcc tattttccct tcgctttttg atgggtttgg
     9541 tatctttctc aatataaaat ataaaataaa gaaaggtaga gcgtgtgttt tgatttgaac
     9601 acgagcggaa aactcggaaa atagataatc tgactgaaaa atcaggattt ctcgtcaggt
     9661 tcctaatttt cagtcgtttt cttctcgctc tttgtatcat aaattatgtc tatccatatt
     9721 gctgctcagc agggtgaaat tgctgataaa attcttcttc ctggggatcc tcttcgtgct
     9781 aagtttattg cggagaattt ccttgatgat gctgtttgtt ttaacgaagt gcgtaacatg
     9841 tttggttaca ctggtactta caagggtcac tgtgtatctg tcatgggaac tgggatggga
     9901 atgccatcta tttcgattta tgcgcgtgag ttaatcgtag actacggtgt gaagaaattg
     9961 attcgtgtgg gaactgcagg ttctttgaat gaagaggttc atgttcgtga attagttttg
    10021 gcgcaggcgg ctgcaaccaa ctcaaacatc gttcgtaatg actggccaca gtacgatttt
    10081 ccacaaattg ctagctttga tttgcttgat aaagcctacc atatcgccaa aaaacttggt
    10141 atgactactc acgttgggaa cgttttgtca tctgatgtct tttactcaaa ttactttgaa
    10201 aagaatatcg agcttggtaa atggggagtc aaggctgtgg aaatggaagc agcagctctt
    10261 tactatcttg ctgcccaata ccatgttgat gcgctagcta tcatgaccat ctctgatagc
    10321 ttggtcaatc cagacgaaga cacaactgca gaagaacgtc aaaatacctt cactgatatg
    10381 atgaaggttg gtttggaaac cttgattgca gaataattat agccaaaaag gaagcccttt
    10441 atgaattgta ggacttcctt ttcttatcca gaaattgatc tagctctctc tgatttcgaa
    10501 gaatagtgac tttatgtgaa tattcttggc aaagtttttg gtaattttct ttttgagttt
    10561 tgctacgccc atcccaaaga atccatctga taaactccca gtcaaagcgt tcagggcaat
    10621 ctgccgccat actttctctg acttttccac ggtatttaag ataacgctta aaggttctaa
    10681 agagacaggt caatggcgaa aaattgagaa agatgatttg gtcagcttct tgcattcgtt
    10741 cttggtagca gcaccaagaa taattaccat cgatgaccca agctttatgc ttggtgagaa
    10801 agttttttat atcggttaac atccattcgt ggtcactgtc ttgccaacca ggttgaaatt
    10861 ggagtgtgtc catgtgcagt tttggaatgg agtagtagtt agataacttt tctgctagag
    10921 ttgacttacc agaaccagaa tatccgataa t
//
LOCUS       DAVLYR010000055        10756 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLYR010000055 DAVLYR010000000
VERSION     DAVLYR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.055"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(30..446)
                     /locus_tag="VYO63_001102"
     CDS             complement(30..446)
                     /locus_tag="VYO63_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672034.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(458..1153)
                     /locus_tag="VYO63_001103"
     CDS             complement(458..1153)
                     /locus_tag="VYO63_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672035.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1347..3848)
                     /locus_tag="VYO63_001104"
     CDS             complement(1347..3848)
                     /locus_tag="VYO63_001104"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1672036.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4330..5418)
                     /locus_tag="VYO63_001105"
     CDS             complement(4330..5418)
                     /locus_tag="VYO63_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1672037.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5436..6104)
                     /locus_tag="VYO63_001106"
     CDS             complement(5436..6104)
                     /locus_tag="VYO63_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1672038.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(6123..8561)
                     /locus_tag="VYO63_001107"
     CDS             complement(6123..8561)
                     /locus_tag="VYO63_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1672039.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(8712..10010)
                     /locus_tag="VYO63_001108"
     CDS             complement(8712..10010)
                     /locus_tag="VYO63_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1672040.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLACQNEIKQS"
     gene            complement(10027..10335)
                     /locus_tag="VYO63_001109"
     CDS             complement(10027..10335)
                     /locus_tag="VYO63_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1672041.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(10381..10701)
                     /locus_tag="VYO63_001110"
     CDS             complement(10381..10701)
                     /locus_tag="VYO63_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1672042.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         3286 a         2344 c         1779 g         3347 t
ORIGIN      
        1 aaattttgct taagaaaaaa caagttttgt tattttttac gattcatcca agtcatccct
       61 atacaattat aggtggataa gatttcaaag cccatagaac gatagaagcc caaggttctt
      121 tctgtctctt ctgtcaccag ctggacttga taggcatctt tgtaatcctc taaagcctct
      181 ttcatcaagg cactaccaat tccttgacgc tgatagattg gtaaaacgat taaatcctga
      241 accaatactg atgagaatcc atctccaacc aaacgaatca agcccaccac agcatcacca
      301 tcaagtgcca gataaattac taatgaatga gataaggcct gctccagcat ctctggttga
      361 tgggtataat ttgtccaacc gacagcctga tagagatgta aaacatcctc tagcttgaca
      421 atttcttgct ttttaatagt aatcatgtca acacctctta aagttctctc aagctcttgt
      481 actgctgtcc atttttatca aaattttcag gacgcaacca tgtttccaaa cgatctttga
      541 cttgaggcca gtccttatca atcatagaca accaatccgt atctctcgta cgtcccttat
      601 aaaccactgc ctgacggaag gttccttcat aaataaagct caaacgctcc gcagctcgtc
      661 tggatggcag atttaaggca tcgcatttcc actcgtagcg acgatagttt agctcctcaa
      721 aaacatagcg agctaggaga tactgagctt cagttcctat ccgtgtaccc cttagctctg
      781 gagaaaaagt gactgctccg acttctatta ctcggttatt ctggtcaatg cgcatgagag
      841 aaaaagatcc caaagcctta ccagtcgcct tgtctatgat tgcatagtaa aaacggtcct
      901 tacgagccaa catctgatgt aaaaggctaa ccagctcccc catatctgct actggctcct
      961 gaaaaaggta ggtccacatc tcctgaggcg tatcaggccc ataaacagct agcaaatcct
     1021 ccgcatgctt ttccactgaa agagcctcta tcctagcata acgcccttct aagaaatcaa
     1081 tagaaggcag ttcacctggt gtataacctt ccattgactc cccaatcatt tgaccatatt
     1141 catttactgg catagctttc tccttttttc acttttcaaa ctttatagtt gcccatagta
     1201 taccatactt tcataagaag attcaaaaaa tcctccagat tctactctga aggattccta
     1261 ccttatttca caacgatatt aacgagttta gttggctcaa acagtttggg aaactgtttg
     1321 aggttggaaa tcagcaaata aattggttat tttacaacaa tattaaccaa tttatttggt
     1381 acactaatca ctttcacgat ttccttaccg tcaatttctg ctttgacttt ttcatccgct
     1441 agagcaattt cttgcaattc ttcacgtgat aggtctttag cgaccatgag tttggcacgg
     1501 actttacctt tgatttggac aacgatttcg atttcgtctt caaccagttt actttcgtcc
     1561 caagttggcc aagctacata agagattgac tcacctgttt ctgcgactgt ttgccagagt
     1621 tcttctgcca agtgaggtgc aaatggtgca atcaattgaa taaagccttt ggcatagtca
     1681 acataaagct tatcttcctt gttagcagca ttgacaaaga ccataagttg ggcaatagct
     1741 gtgttgaatt tgagagactc aatttgctca gtaacagctt tgactgtttc gttgtaaacc
     1801 ttgtcaagag caccattgtt ttccgcaagg atttctttac ttgtaatcaa acggtaaact
     1861 cggtcaagga acttacggct tccttccaaa ccttcttctg accaagcaat cgaagcatcg
     1921 agtggtccca taaacatttc ataaacacga agggtatcgg caccgtattg ttccaccaca
     1981 tcgtctgggt taacaacgtt cttgagcgat ttagacatct tggctggcgc ttgctccaac
     2041 tcttcccctg tttctacatg gaagaaggaa ccatcacgtt tttcaacctt gtcggttgcc
     2101 acaagagcac cacggtggtc acggtagctt gttcccaaaa tcatcccttg gttaaagagt
     2161 ttttggaatg gttccttagt cggaacaaca ccgaggtcat agaggaattt gtgccagaaa
     2221 cgagcgtaaa gcaagtgaag aacagcatgc tctgcaccac ccacgtagat atctactggc
     2281 aaccattgtt tgaggaggtc ctcatcagcc aacttctcag tattgtgtgg atcaatatag
     2341 cggaggtagt accagcttga accagcccat tgtggcatag tgttggtttc acgacgacct
     2401 ttgacaccat cttcacgagt cacttcaagc caatctgtca agttagctag tggactttca
     2461 ccagtacctg aaggacggat atccttggtt acaggcaaga caagcggcaa ttcagtttca
     2521 ggaacagctg ttgaagttcc atcttcccaa tgaatgattg gaattggctc accccagtaa
     2581 cgttgacggc taaagagcca gtcgcggaga cggtaggtaa ccttctcctg accacagcct
     2641 ttttcttcca accaagccac aatcttggca atagcgtctt ctttgttgag tccatctagg
     2701 aagtctgaat tgacatgcag gccatcctct gtgtaggcag cttcttcgac atttccacct
     2761 tcaagtactt cgacgattgg aaggtcaaat tgtttggcaa attcccagtc acgttggtcg
     2821 tgggcaggca cagccataac cgcacctgtt ccataactag caaggacata gtctgcaatc
     2881 cagattggca tttccttacc attgacaggg ttgatggcat aagcaccagt ccaaacccct
     2941 gttttttctt tagcaaggtc tgtacgagcc aagtcagact taaggctggc ttggtgttta
     3001 tagtctgcta cagcttctgc ttgctctgaa cttgtgatag cgtctactaa ttcatgttca
     3061 ggagccaaga cagtgaaagt cgcaccgaaa agtgtgtccg gacgagtagt aaagactgta
     3121 aattccttgt ctgttccttt tactttgaaa gttacattgg caccagttga tttaccaatc
     3181 cagttgcgtt gcatatcctt gatagactct gaccaatcta gttcatctaa gtcattgagc
     3241 aagcgctctg cgtaagccgt gattttgagc atccattggc gcattggttt gcggacaact
     3301 ggatagcctc cacgctcaga agttccgtca ggaagcactt cttcattggc aatggcagtt
     3361 cccaattcct caacccagtt tactggcact tcagcttcat aggccaagcc tttttcgtaa
     3421 agcttggtga aaatccattg agtccacttg tagtagtttg gatctgttgt gttgacttca
     3481 cgatcccagt cataagaaaa tccaagcgca ttaatttgac gtttgaagtt ggcaatgttt
     3541 tccgctgtaa attctgctgg gtcattacca gtatccatag cgtattgctc tgcaggcaaa
     3601 ccaaaagcat cccaacccat tgggtgaagg acattgtagc cttgcgcacg tttgtaacga
     3661 ctgaggatat cggttgcagt ataaccttct gggtgtccta cgtgcagacc agctccagac
     3721 ggataaggga acatatcaag cgcataaaac ttaggttttg atgcatctgt tcctgtctta
     3781 aatgtatgat gttctgccca gtagccctgc cacttaggct caatttcttt atgattgtaa
     3841 aaactcatgg tctctctcca attttgtgat gttactatta taccattttt gagggaattt
     3901 gaaatgtgtt taaaggaaaa actgtatact gtatctcatt aattttgatg actatgaatt
     3961 gctctatctc ccacttttta tcccattgtt atgcagtaaa tcaaggagta agtcaagaag
     4021 cttatctcgt atagcacaaa atacttattt tagaccgaag ataaaactgg aatgctaatt
     4081 aatgtattca caataaatag ccgacaacct atgtcgcttt ttgattgccg acaacctatg
     4141 tcgctttttg atttatgaag aacatatagt agactgaatc tgaaatagta cgaaacaatt
     4201 gctaaaacat ttatagaaat taattttact ttcccaatcg atttgttctc atcctatttc
     4261 aatcttctat agaattcaat acagggatca ataaaagact accatttgga aaacagtagt
     4321 ccttattgtt tattttgaat ttacataggc atctacagcg ataatagctt gagcaacatc
     4381 tgaaggcgaa atcttaaacg gcatctgatg aattgtctca ccctccatag ttgcttgttt
     4441 accaactttt attaaatcat catatccaac ttgatccaaa tgcatttctt ttagagttgt
     4501 tggcatacca atttttttgt aaaactcaat atacttatca agttcttctt taggtctatt
     4561 ttccaataat agttgtacta aagttccata agctactttt tcaccatgtg ttaaatgatg
     4621 aatgtcacct gtcaatgcag taaaaccatt atgaattgca tgcgccgcag ctaatcctcc
     4681 actttcaaaa cctagaccac tcaataaagt attagcttca acaatatttt ctaatgctgg
     4741 tgtcaccact ttagcttcac aagctgccat agcctgtaaa ccatctgcaa acagcgtttc
     4801 ttcacatttc ttcgcaattg caactccagc caatgtttgc tgttgtccca acatagtttt
     4861 tccatttgcc tgcataaccg cacgcgcctc aacccaagtt gctaaaccat ctgcaatacc
     4921 agacgctaat aaacgcttag gggcttgtga aataactttt gtatcaacca aaactaaatc
     4981 tggattttta gaataaaata gataatgatc aaatgcacct tcatctgtat aaataacaga
     5041 taaagcagat acaggtgcgt cggtcgatgc aattgttgga gcaataataa caggcttttc
     5101 aatcaaatct gcaatagctt ttgcgctatc aatcgtcttt cccccaccaa gaccgataat
     5161 actatcacaa ttttctttct cagccaaggc aacaactcga ttgatttcat tgtcagaagc
     5221 ttcaccatta aatagcgcca gaacaatatg gaaaccatac ctatgtaggt aatcttcaaa
     5281 tcgttttcca acaatatcat aaaccaactg atcgcataat agaatagggc aatttcccaa
     5341 atccaaaatt gatttggcat tttcaaacaa ggcattttcc ccctgaatat atctagaagg
     5401 actagcaaaa attctcatat gtatttcctc tctatctaaa tggaacgact attttgaata
     5461 acaaaccaat cgtcagaaaa atcatcaacc gccttttgga tagatggcat ggcgaaagct
     5521 gattcaaaaa catccgctcc tgctgtaaca gcatgcgcac ctgcagctaa agcattattt
     5581 acttgtgcta catttttaaa ggatgcagct aaaatcttac taggagagtt ctgtctatca
     5641 atagcaagag ctaattgacg aatgacagaa tttgaatcaa tgttcagatt ttccattcta
     5701 ttataatatg gagctaggta atccgctcct gcttcgatag ctaataatcc ctgaataact
     5761 gtataaatag ctgttgcagt gatatggtag ccctcttttt ttagcgcctt tattgcacgt
     5821 aatccagctg gagtaacagg tactttgata aatatatcat ctcctgcttg tcttcgaatt
     5881 ttatgagcat cctttaagat gccttcaaaa tcttgagaaa tcacctgaac atgaatagag
     5941 ggtgtagagc caatcaattc tcttacatct ttgattcgtt caaaaaaatt aatagaaccc
     6001 tctctttttg caatagtggg atttgaagtt accccagcta gcggcaaaat ttcagaccac
     6061 tttttaatct catctaaatt taatgtgtca agcataaatt ccataaaaag aacctcttta
     6121 ttttacaaag tatgctcagt acgtcctata atgtcatctt gggttgcctt agaaagaaca
     6181 ttgaagaatg cagagtatcc tgcaacacga acaattaagt ctctgtgttt ttcaggatgt
     6241 ttctgagcgt caatcagcgt ctctctggaa acaacattgt attgaatatg gtacccatgt
     6301 aaacgattaa agaatgttcg taacaaagca attagtttta atttatcttc ttctttggct
     6361 aacgtttgag gatttacttt ctgatttaag agaaccccac ctacgatttc atctgttggt
     6421 aattttgaaa cagattttaa aacagatgta gggccgtgtt gatccatatt atgtgatggt
     6481 gaacaaccct ctgctaacgg tgtacccgcg ttgcgtccat ctggagttgc taatgttcca
     6541 cgtccctgcc ctacgttggc tgagatagaa gatgttcctg aataacgaat tcctccaatt
     6601 ggacctcttc cataacgtgt attaggatat ttagcaattt catcaacata aatgtcataa
     6661 gcagcagtaa ccaatttgtc agcataatca tcatcattac catacttagg tgcatcatga
     6721 atcaacattt cttgaatgac cttaccttct tctccggcat aatctgtttc cagtgcatgc
     6781 caaagctgac ttgggcttat acgttcttcc tcaaacacca attttttaat tgcagctaat
     6841 gaatccgaca aatttgcaat tccaacttgc aatcctgata tataatcata tactgctcca
     6901 ccttctttaa ggtgttttcc acgaccaata caatcatcag tcaatgctga acatagaata
     6961 tcaggaactt ctcgttccaa tgataaatca atagaatttt caacaataac actcattcgt
     7021 gtcaaatatc ttagtgtttt atcccaagca ttttctaatt cagaaaagtt cttcatatcc
     7081 ttaaaatgac caaagcttgg tgcaaaccgt ttacccgaag ccggatcaat tccatcattc
     7141 atcgtgataa gtagaacctt agggaagttc atataactca tacctgtgca acgatagccc
     7201 catttccctg gaactgccgt ttcaacacat ccaatggcac tgtaatcata agcatcatct
     7261 tccaatactc cttttgcaat aaaagaagga ataataatct catcattatt aaatgcaggc
     7321 ataccaaaac caagtttcat cacttcaata cactcattca tgaaacgagc atctaaacct
     7381 gcatggtaac gtacagttag attaggttgc ggtagatggg tttgtgcaac tgattttaat
     7441 accaaataag ataatgggtt aacagcatcc ttcttatctc gagtctgtcc accaattgta
     7501 acattttgat ataaaggact tcctgctgaa gaaaatgtat gtgattgact gcgaacctta
     7561 ttaattgtaa ttgtcttaat ccaaagattt gtcagacgtt caacaatgct atcttctgtt
     7621 tctttaccac tttctaaatc agccttcata tatggataca tatattgatc aaaacggcca
     7681 tatgaaagag agtggccatt agattcaatt tgtaaaatac attgaataaa ccaaactgat
     7741 tgaatagctt ctgcaaaagt agttgccggt tcatatggga ctctagagca aatatctgca
     7801 atctcaagta attctttctt acgtttagga tttgcatttt cggctaaact tttagcaaga
     7861 gcaacaaagc gctttgcata tactttaata gcatcgatta cgataaatat agagtcgtaa
     7921 aaatgatatt tatcaatact tgctggatct gttaaatcta gagctacctt cgctttacga
     7981 gcccgctctt caaaacctct taaaccaaat tgcaaaagtt tctgatagtt aactgctaag
     8041 tgagcatctc cagaattcat cttaccttcc ataccgaaga atcctgtttc catataaaca
     8101 gacacttctt caggtaataa ggcaccagct ctagcacgta aattattatt ttcccaaaac
     8161 ggagcaatac ttctaagttg ttcttttgtt tcttctgtaa tatagaaaac atctccatca
     8221 cgcttttcaa aaagatccaa ctcattgaga acaaattcta gcgtatattc cggaaaaata
     8281 ggagcatctt tattggaaga agcttgattt cccgcaatca tagattcttc ttcaatatag
     8341 atagacatat tttccaaaat ttctttcagc atatatgcac gttttaggac attaggttgt
     8401 tccttgtatc gttcataggc gcgtgttgct agaactgctc tctcagcatc aatataaggt
     8461 tttttattta aaacatcttc tcgatatttg ttcatccttt cagtaaggct gccaaaatat
     8521 tctgtcttga ttgttgttct tgctacttct gtatttacca tcgtaagcac ctttctttcg
     8581 aaaatatttt tatttctttc gaaaatataa taccgatttt tatagaaatt ttcaagcttt
     8641 taactagaaa aattttaact ctctaatttt tagcacaaat gtgtgagttt aacagtaaca
     8701 cacaaatacc tctaagattg tttgatttca ttttggcaag ctaaacgatc ttgtatctta
     8761 aagaatggaa agtaaaccat tgtagacatc gccaatacta ataactgagt cacaacacct
     8821 tgccatccac ccaccaaaaa tcctgataga atagctggtg tactccaagg caatgttacc
     8881 cctgagaatg gttgcatgaa acctgttgca atagctccat atactatcac agctgcaagt
     8941 acaggaacaa gaatgaaagg tacaaacata actggattca tgacaatcgg aaatccaaat
     9001 acaactggct catttacgtt aaatattgct ggaaaagctg caacttttcc taaggcttgg
     9061 tattgttttg attttgctgc aaaaagcatg gcaactacaa gaccaaacgt aatccctgaa
     9121 cctgatagaa ttaaaaatga atctaaaaat tgttgagtaa caatatgtgc accattttct
     9181 aatgatagat taccagaggc taacatagct ttattagcat caagattaga taaaagcaga
     9241 gctgtcacta ctccatttac taccgattgc ccatgaacac caaaccacca caaaaatgat
     9301 ataaagaatg caattccaat agcaccatac aaagatccag ttaaaccttg caacggaact
     9361 tgaatagcag aataaatcat ttctatgaat gttccgccat tagtcaatga cttcgctaaa
     9421 atatatacaa tcatagaaga taagaaaatt acaaatgctg gaatcattgc ttcaaactgt
     9481 ttggcaatag cttgtggaac ttgttctggc atcttaataa caatttttct ctttataaag
     9541 aaggtataaa tacttcctac taccaaacct ataatgatag caccgataat tccttggcct
     9601 ccaaaccaaa ctttactaat agcgtcccca atcgcctcac cttgtttagg gatataagat
     9661 gatcttagca aaataaagaa tgcagataca gatagaactc cagctggtaa agcctctact
     9721 ccgctattct tagcataaga ataggcaatt gaaaaacaag aaattagacc cataatagca
     9781 aaagttcctg aatatacttg cataaacggc tctgtccaat tagctccaaa aacactagca
     9841 atgctcttat ttaatccttc gaacggcaat tgtcccataa tcaagaacaa actgccaact
     9901 actgtcaatg gcaaaattgc taacatccca tcttttagag ctataatgcc acgcatattc
     9961 acaaacttca tcatcggtgc aatgattttc tgaacatcca tctttgacat aataaatctc
    10021 cttttcttac ccactaatca aagatagggc caaatctaat acttttttcc catctaacat
    10081 accatagtcc atcatcggaa taacagctat cggaacatca cacttatcac aaatttcttt
    10141 tgatttatct aatgtataag caacttgtgg acccaatagt gcaacatcta tatttggcgc
    10201 ataatccgct aatttagact gagaaaacgc ctctatttct gcctcaactc cactagattg
    10261 cgctgcaatt ttcatattat ttacaagcat accagtagaa aaacctgctg cacaaaacaa
    10321 accaatcttc accattatgt tttcctcctc tatgttaata acaatgataa tactctagta
    10381 ttatttttta tgaagttctt ttctcaaact aataatttct ttgattaaat taatctccgt
    10441 catgctagtc atgagatgat cttgtgaatg aacaaagaga gctgtaattt ctgtctttgt
    10501 accagacgct tcttgtgcca agaattttgt ttgtagatta tgggcttcta ataaggcggc
    10561 atcagctaac tggatttctt cttcacaccg ttcacatgta ccatttttta tgtaatctaa
    10621 tgctttataa atatgttgtt tcgcatcgcc agcatataaa attaaaccca tgataatttg
    10681 atctggaaca ataatttcca taaattcctc ctcatttcac tatgtaaaaa gcttttttca
    10741 tttgaaattt taaaat
//
LOCUS       DAVLYR010000056        10648 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLYR010000056 DAVLYR010000000
VERSION     DAVLYR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.056"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..614
                     /locus_tag="VYO63_001111"
                     /pseudo
     CDS             1..614
                     /locus_tag="VYO63_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(742..1764)
                     /gene="cbpF"
                     /locus_tag="VYO63_001112"
                     /pseudo
     CDS             complement(742..1764)
                     /gene="cbpF"
                     /locus_tag="VYO63_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1783..2604)
                     /gene="cbpG"
                     /locus_tag="VYO63_001113"
     CDS             complement(1783..2604)
                     /gene="cbpG"
                     /locus_tag="VYO63_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV1672043.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2685..2924)
                     /locus_tag="VYO63_001114"
     CDS             complement(2685..2924)
                     /locus_tag="VYO63_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672044.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3074..3340)
                     /locus_tag="VYO63_001115"
                     /pseudo
     CDS             complement(3074..3340)
                     /locus_tag="VYO63_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3365..3676)
                     /locus_tag="VYO63_001116"
     CDS             complement(3365..3676)
                     /locus_tag="VYO63_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1672045.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLT
                     S"
     gene            complement(3601..3843)
                     /locus_tag="VYO63_001117"
     CDS             complement(3601..3843)
                     /locus_tag="VYO63_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1672046.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(3844..4476)
                     /locus_tag="VYO63_001118"
     CDS             complement(3844..4476)
                     /locus_tag="VYO63_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672047.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4490..5485)
                     /locus_tag="VYO63_001119"
     CDS             complement(4490..5485)
                     /locus_tag="VYO63_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1672048.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDQDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5482..6180)
                     /locus_tag="VYO63_001120"
     CDS             complement(5482..6180)
                     /locus_tag="VYO63_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1672049.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6258..7268)
                     /locus_tag="VYO63_001121"
     CDS             complement(6258..7268)
                     /locus_tag="VYO63_001121"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1672050.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7252..8259)
                     /locus_tag="VYO63_001122"
     CDS             complement(7252..8259)
                     /locus_tag="VYO63_001122"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1672051.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAESTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8246..9199)
                     /gene="mvaD"
                     /locus_tag="VYO63_001123"
     CDS             complement(8246..9199)
                     /gene="mvaD"
                     /locus_tag="VYO63_001123"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1672052.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9181..10059)
                     /gene="mvk"
                     /locus_tag="VYO63_001124"
     CDS             complement(9181..10059)
                     /gene="mvk"
                     /locus_tag="VYO63_001124"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1672053.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10234..10512)
                     /locus_tag="VYO63_001125"
     CDS             complement(10234..10512)
                     /locus_tag="VYO63_001125"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672054.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
BASE COUNT         3146 a         2469 c         1879 g         3154 t
ORIGIN      
        1 aaaccagata aggaccaaga gcttaaagct gaaattcaat ccatttttat cgaacacaag
       61 ggaaattata cttatcgccg ggttcattta gaactaagaa atcgtggtta tctggtaaat
      121 cataaaagag ttcaaggctt gatgaaagta ctcaatttac aagctaaaat gcgacagaaa
      181 cgaaaataaa ggagacgttg gtaagaaggc agagaatctc attcaaggcc aatttgaagg
      241 ctctaaaaca atggaaaagt gctacacaga tgtgacagaa tttgccattc cagcaagtac
      301 tcaaaagctt tacttatcac cagttttaga tggctttaac agtgaaatta tcgcctataa
      361 tctttcaact tcacccaact tagaacaagt acaaacaatg ttggaacagg cattcacaga
      421 gaagcactat ggtggagtcc ttctttggga ttctgaaatc ggaaatgttt tatggctatg
      481 agaaaacatt taaatcactt aatcaattgg aacaagctat tatagactat attgattatt
      541 acaacaacaa gagaattaag gtaaaactaa aaggacttag tcctgtgcag tacagaacta
      601 aatcctttgg ataaattatt tgtctaactt ttggggggca gtacagaaaa accttgatac
      661 tatgcgtttt ttgattgtga agacttttat ccacctatta tacaaagtta agtatccatc
      721 acagtttaca attagccttc attacttaac ccattcacca ttatagttta agtagtaacc
      781 acctactgtg gtattaacag ctaaagcacc tgaatcatag gcatagtacc agttaccatt
      841 gacttggaac cagcctgttt tcatatctcc attacttgca tttagatagt accaagtcga
      901 accttcctga taccaaccag ttgccatagc tcctgatgaa cggagatagt accacttgtt
      961 accaaggtat tgccagccag ttctcatatc accattttca gcatctagat agtaccaagt
     1021 tgagccttcc ttataccagc cagttgccat agctcctgac gaatggaggt agtaccatct
     1081 attacctaga aattgccaac cagtttgcat aatgccagtt gttggatcta gatagtacca
     1141 tggagctgct tttagcttct cttcatcata ggtaagaggg gaatccttaa cccaaccacg
     1201 tgctagctct ccaaccgcca atctgttgat gcgcgaatca aagccaccat ccttctgtaa
     1261 ataataccaa tgaccctctt cgtaaatcca accagttttt aaagtatgat aactacgctg
     1321 atcttcaaaa taatagactc atttctctgc tgggctatca tattcttccc catgatgttt
     1381 gttggtattc gtagcagttt ttgcttctaa aacttgcttg ccaacaaatt cttgtaatac
     1441 accatcttga ccaaaataaa accaatctgg tctaagagaa atctctattc ttggagaagg
     1501 accaatcgta acccccttgt gtggagcagg tatatattgc cagccgacaa ccatctctcc
     1561 ggataaagga tcaaaataat agtacttccc atctatcact ctccagtagg tttcttttag
     1621 atcccccttt ttgtagtagg ttctaccatt ttcttggaca aactgccatc cttcagaatc
     1681 atctgcaaat actgtactgg tccctagcaa accgaagaaa aatgtggcta gtgcgacttg
     1741 catcattttt ttcaaaagct tcattttata tatcctccaa cattaaatcc actcaccaga
     1801 tgaagcgaag ttataaactt taccatcgat agtttggcta cctgtaacca ttgctccatt
     1861 tgaattgaga taataccatt ttccatttac tttttgccaa tctgtaagca tcttaccgga
     1921 actgtctaaa taataccaag tatcatttat ctcctgccaa cccgtttgtt tatcatgttg
     1981 tctataatgg taccaactac catttatttt cttccatcct tcaagagagt aacctttaag
     2041 aaccgaataa ataaatggca aatttcgttc atttaattta actgcactgt taatttgatt
     2101 agctccatct cctaaagtat gcactcctac tacacggtga ctagcatcat aaactgtaga
     2161 tccactagac ccctctaaag tatcaacttg gtaatccaag aacatgccat catcacttaa
     2221 aacttgtttt ttatctgtat acatttgatg aattttaaaa ttatatgatg gatagcctgt
     2281 gatagtcaca gttattcctg tcaaattttt ttgactagta ggaagaccca aagtccctaa
     2341 ttttgcacca atgggctctt ctagaattaa taaagccaag tcatattccc ttgcatcctt
     2401 agaatttaaa tttctaaatt ccttcaaata acgaacttcc tttactttga tctttccaaa
     2461 tagttcttga cttggactaa cagccggaag aacataaata tcatccgctt ctttcccata
     2521 gtcatgtctg tagtagttgt gagccgctgt aattaacaca ttatctttaa taaaattcgc
     2581 acttcccctc ccttctacat tcattccatc ggctactcca taatacttag ataaaaccat
     2641 agctgaagtc gaatacggat actgtaaagt attatcaatt ttaatcaaat catcattacc
     2701 gataatactt ctgattgctt ttggtagtat gaaccatacg ttggtgaaat ctcagataat
     2761 gaagaatcat tagatcctgg acctttttct agtgtctcac ttacctcata ttcttcaccc
     2821 gtactagaaa taacactcaa agcagatact gtcgataact ggctagccaa taaagtactc
     2881 gcaataattg aaatacccaa ttttttataa acagttttct tcattattgt agcctcctaa
     2941 tgtaattata gcctactatt ctaaatttct taatctacta tagaatcaag aaatctacca
     3001 ccttctttaa ataccctcca ttatcacata aacaggtaaa cttttcaatt aatgactgcg
     3061 cttttcaatc acgctagagg tacttgcttg cttctttgat actaagttca gccattcttt
     3121 ccttgttttc atcaataaag catgttaccc aagttggatt cgttttggag tagtttcgca
     3181 gaatccagcc aatggcttta ttgataaaaa attctgtttg gttcaagtta tgaagtagaa
     3241 tcttttccat taattgagta ttggtcttct cttttcttaa caactggtgg tcaatagcga
     3301 cacgtctcag ccagatatta tctgataggc tccattttat actcaatgaa aatcaaagag
     3361 caaactagga agttagccgc agattgctca aaacactgtt ttgaggttgc agatagagct
     3421 gacgtggttt gaagagattt tcgaagagta ttaagattat ttcttctagt tcagggtgtt
     3481 catacaccaa actccctact actcgatcta ggatatctac cgtgtcccac caggattttg
     3541 tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt
     3601 tcaaatagtt agcagccaca tattggtatt ttctaggctc cttttcccag caagtgtctg
     3661 caaaatccca atcgataatc tttgtttttt tcgcttctgg aaaatatttt tatagagttt
     3721 atttctttca ggcaccgcaa tacctagaaa agaaaattga tggcgcatat aggcttccat
     3781 ggaccttgct tttttagagt cttttgctgc ttctagctcc tcaagtaaat ctgctaaact
     3841 catctaaaac tcctcttgcc ccaccaaatg gtgctgaaag gcatagacag ccgcctgggt
     3901 acgatcgctg acttcaagtt tggcaagaat attggacacg tgggtcttga ccgtcttgag
     3961 agagataaag aggtcatctg cgatgcgctg attttcgtag cccttggcga tgagttggag
     4021 aacatctcgc tcacgcgcag tcaattcttc atgaagttcc atatgattgc ggtggtattc
     4081 aaccttcttg ctaacctctt gctcaatggc cagctcgcca gcagctacct tactgacggc
     4141 atgaagcaat tcatctgcac tagaagtctt gagcatatag cctttggcac cagcatctaa
     4201 gactggcatg attttttcat tgtccaaata agaggtcaca atcaaaatct tggcttcagg
     4261 ccattcttta aggattgcta aggtcgcgtc aatcccattc atctcaggca tgacaatatc
     4321 catgacaatg acatctggac gcagttccaa ggccaagtca atcccttgag acccgttgga
     4381 cgcctcaccc acaacttcta catcgtcttg gaggtcaaag tagcttttca aacccaaacg
     4441 gaccatttca tggtcatcta ctagtaaaat tttcatcttt actcctttat cattccttat
     4501 ctaacagggg aatacggata tcaactgcca gcccttgctt gggagctgtt aataactgaa
     4561 ccgtccctgc catatcttca acccgctcct tgatatttcg cagtccataa ctcaagtcgt
     4621 ctaagctccc taactggaaa ccaatcccat tgtccaccac cttcagttgc aattcaacat
     4681 ctgtctgata gaggtagaca tctaggcaag atgcctgggc atggcggagg gtattgctaa
     4741 tcaactcttg caggatacgg aagatatgct cctcgatttt cttaggcaat ttcgtcatat
     4801 tctgcttgag actaacccta agatcactct tgtcctcaag ctcttttaag agaatttgaa
     4861 tcccttctat caagctcttc tgctccagtt caactggtcg caaatgcaag agcaaaaccc
     4921 gcaaatcctt ctgggctgtt tctaaaatag ctgtgacact ctgcaactgg gtctgcatct
     4981 tttctctatc caatttcaaa gcctgctgac tgatacccga taaaatcatg tgggccgcaa
     5041 acaactcctg actgactgta tcgtgcaaat cccgagcaat tcgcttccgt tccttctcga
     5101 tgatttcctc ttcctgagca aggctatgat tttcagcttt ttgaagagcc tctgtcaaaa
     5161 ggttaagttt acctgataag gacttgaaac tggcatccaa atcttgatct acaacctgaa
     5221 ctacttcttg ccctgccaat aaacgcttga gattagcctg catttttctt agagaaagct
     5281 cttcgatccc tcgccaaaac agggctaaga gacaggtcat ggacatgctg aaaaccaaca
     5341 ataaaaagac aaatttttct gttttttcga catcgtgcaa aaagatagac cagtcaaaat
     5401 caagtatttc cagcaagctg tgggagaaaa aaaagacaaa taggaaggag gtgagagcaa
     5461 taatgacata ggcttgtttt ttcatcctct aaccacctcc acatcaccaa tcatagtggt
     5521 caagaaaatc ttgacactct tgttactctt gagatagtct tttgtttctt gatgatagtg
     5581 ttcattgcgg agggctcgct tgggctggtt gaaaaaagtc aaatccccat agagacagtt
     5641 aacgctgaga ctgacttcca catctacagg tacgatgatt ttggtcgttc ctaccatctt
     5701 tctgaggata atgacattgt catgattggt taagatgacc ctctccagat gaatagtgtc
     5761 cttacccatg aagcgaaaga gattgatatc atcgaattgg caagtctggt agcttgaaaa
     5821 atgatgaaga tttccaaacc aacgattttt ctccttctta accgtcacga cctcttcaaa
     5881 aaccaaattg gtctgctctt tttcctggtt catcatcggg taaagaagaa agaggctata
     5941 gataaccgca acaaaaatag ctagaatcac aaaaggattg agcataacga tgaaaaagaa
     6001 gagaatggtt gccgctacta aaagaagatt atttccctct ttaccagtgt agtagcgaat
     6061 caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa aaatgctctg ataccatcaa
     6121 aatcagagct cctgtcagaa gacaggcttc gataaataaa aagattttaa attttctcat
     6181 aggttcatcc tctcccttct attttatcac aattcaaaaa agtcacctca gtctgaggat
     6241 ggaaaaaaag gcgctggtta cgcctttttc atctgatcct ttgcttcttt taattttcca
     6301 taaagaagat agtctacttt ttgtagatct gctatggtgg cacagttaag ggaacacata
     6361 atcaagcgta gatctgcttt ccagccttgg acaatgccaa tcacttcttc aactgtgtag
     6421 gtttcaacca attccagaac ggttcgtgac aatcccacag ccttagcacc aaaaaccaag
     6481 cacttaatca tatccagcgg attccgaacc cctccactaa ccaagagttc gaccttatct
     6541 ttccattctt gggcattgag aagggcctgc atggtagact gaccccattg attgaggtaa
     6601 tcacgctggc cactacgacg gttttcgata taggcaaagc tggtgccacc acgacccgat
     6661 aggtccactg tacgaacacc gaattcatag gctctttcga ttgtcttggc atccattcca
     6721 aagcccactt ccttgaggac aataggaacg ggaatttgct tgctataatc tgctagatgc
     6781 gattgccagc ttctaaactt cctttctccc tcgggcatga gtaattcctg catgacattg
     6841 acatgcactt gcaatagaac aggattcatc tcttctacag tctgaagtcc taactcgaca
     6901 ggtttgtcca atccaatatt ggttccaagg aggagattgg gatgactaga cttgacagaa
     6961 aaagaatcat ccgttggatt tttgagggct gcgctataag aacccgttac aaataaaata
     7021 ccacaggatt ccgccacctg agccagcttt tgattgattt ctcttccctt attacttcca
     7081 ccagtcatgg cattgatata aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa
     7141 agatcgattt catccagatt gtaaagaggc aaggaagaat gaatcagctc cacctcatca
     7201 aagctattat aggaactttt ctgctcaagg gcatagagga tatgctcgtc cttacgattt
     7261 gtcgtcatgt cctatccttt cttgatataa gagctcaatc cccagatcgg cccaacgatt
     7321 ttttaaggtt ttggttgatt ccgcatcaaa actcagggcg atgccacagt caccaccacc
     7381 agcaccacta ctcttggcaa cggcctgcaa atcttgactg gcttctttca actgtctaag
     7441 caaaggcgtg taaatatctg tactcaagcc ttctaaaagc ttgctggcta cttctacttg
     7501 ctcgataact ttttcggctt tcccctgttc caaggcttcg accaaagaaa ccaccgtttc
     7561 ttttgaggaa cttaaaaaat tttgattgat attttgcttg atttgctgga ccatgtgact
     7621 cgatacagcc acttccttgg tccatcccac taagaaatca cattctaaag ttggtttcac
     7681 ttgtgaaatt gaaaagcccc aatcacgctc cagaactgtc gccaagtttt cttcttctaa
     7741 ccaagcagcc accttctggc gatcaaatga ctggtagaga accaaatcct ctgccacaat
     7801 acaggcaagg tcgcccatgg aaccattgtc tcctcgctta agcaagacag cgctagtcag
     7861 cttgaacaag agctcctgat caacagaaac atcatacaga gccagtaaag ccttgacaac
     7921 caagacaacg acgctgccac tagaacctag accaaacttt ttcccttctc gttccatttt
     7981 gccacagatt tctagagaaa aaggtcttaa attctgacca cgaacagcga ggaagtctcc
     8041 catcaaagca atcgtttctt gaatcaagct atagtcagga ttaggcctta agtccactgc
     8101 gaaatcaaac atatctgaat agatacggta gctgtcagaa aaagcaatct cagccctcat
     8161 atagatggga atatccttta tcaaagctaa ctgccctggc tctaaaatag catattcacc
     8221 tgcccaatag agttttccgc aagttttaac agcaatcatc ttgactcaaa tcctttgttt
     8281 ttgacacaat caagcgataa cgatgaccga aaatttctga taaatgctcc aagtctttct
     8341 cctgacagaa gaccttaaca ttgggaccag catccatggt aaagtagcag gcctctcctt
     8401 tctcacgaag ctggcgaaca aagtccatag cctcataaga ggcatccgtc agataagaaa
     8461 aggctggact agcagtcttt gtcgtagcat gcatagccag ggcatttttc tccgttaatt
     8521 ctccaatctt ggcaaaatca ttttccttga gataaatcag catatcctga tagtccttct
     8581 cagactgacg aacccagtcg tcgaaagtcg tcgaggtttc cacacaaagt ttcatcccgt
     8641 cacggctaga gattggtttt ttcttgtcct ctagcaccaa cataatcata gctagtttca
     8701 agtctgtctc tacagggtaa atttctccac tatccttatc ccaggctcct agtggtccat
     8761 aaaaactccg agaagaagaa cctgaggcaa atttggcttc ctgtgccaac tgacttctat
     8821 ccaatccaag cttgaaataa gcattacaag ccttgaccag ggcggacaaa ccactagaac
     8881 ttgaggacag acccgctgcc gtaggcatat tgttttgagt atcgatacgg acaaagccct
     8941 caccagctgg acgataacgg tcaataatct tactcatctt ggcatgctcg acctcatttt
     9001 gtagctgacc attgatgtaa aattcgtcag ctgttacatt ggctggtaaa ggcgacaagg
     9061 tcgtctctgt atacatattt tccaaagtta gagaaatact gctagtagca ggcaccatct
     9121 ctttttcttt tttctttccc caatatttga taatagcaat atttgcgtag gaacgtactg
     9181 ttacaggctc tctatccatg tctgaacagc tcctttctct tctaatcttt ctgctagttc
     9241 ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa cctcctagcc caccaccgct
     9301 catcttggca cccagagcac catggctaag agtcgtttca accaaaaagt ctgcctcagg
     9361 gctactgact ccaatttctt ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc
     9421 ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg
     9481 caaaaacggt agggcatcct tgcccttatt ttgaaccact tggatggctt cacgagtatg
     9541 accataaaca cccgtatcgg caatcaccaa ataggcggat aaatccatct caagttctgt
     9601 aaatcctacg ttcttgataa agcgaatagg ttggtcacta agacaggtct tagcatccaa
     9661 accactagga ttcatatggg caatcatttc agctcgattg accaagattt ctagtacatc
     9721 atgaggcaga tcagcctgat agtagtcaaa tactgcacga atggccgcta tgctgatagc
     9781 cgctgacgaa cccatccccc gtttctcagg gatagccgag tcaatctcac aacgaatgca
     9841 ggcttctgtg atattcaaat actccagtga ggcataaacc gccatggaca aggtatcctc
     9901 ctcataaagg cgccaaggac tctctgcagg aactacctta caggtcacct ccacctccaa
     9961 aagaggcagg gaaatggcag gataaccgta aacgaccgca tgttccccta ttaaaattat
    10021 cttactatgt gcctgaccga caccaacttt ttttgtcatt ttttcctttt actagacgaa
    10081 aaaacgtctt atttttcata caagtattaa ttctttccta tctattttat tatattttca
    10141 caaaaaaagc gattgtttcc attcacaatc gcttctttca ttattgaacc cattcgccat
    10201 tatagttgac agaatagcca tctacggtcg tattcactgc caaggcacct gagcgctata
    10261 agcgtagtac catctgccat tgacctggaa ccaacctgtc gtcatagaac gacgaaagaa
    10321 actccatacc attaagtaaa gaggaaagtc gtgagggagc atgcgccatt gacaacctgt
    10381 tttagtgacg tacaaagtct cattaacaag tactcgtttc ggccatttat aggtgcggtg
    10441 tttggagaaa tagggttcaa tcttcgccca ttcttgatcg tttaaatcag tatcatatgc
    10501 tttgcgtatc ataactctag cttaacattt ttttgtgaat acaggttcta aataatcgac
    10561 cacgaaaatt tcttaagtgg aaaacgcctt atgaagtatg ctacgggaaa cttatgcact
    10621 taatttgaca attcaagatg taaaaata
//
LOCUS       DAVLYR010000057        10615 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLYR010000057 DAVLYR010000000
VERSION     DAVLYR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.057"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            16..459
                     /locus_tag="VYO63_001126"
     CDS             16..459
                     /locus_tag="VYO63_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1672055.1"
                     /translation="MFCLEADNQEQLFDQVATLLEEREIVTPTYREALITREKSFPTG
                     LDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFII
                     NNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            497..802
                     /locus_tag="VYO63_001127"
     CDS             497..802
                     /locus_tag="VYO63_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1672056.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            850..2325
                     /locus_tag="VYO63_001128"
     CDS             850..2325
                     /locus_tag="VYO63_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1672057.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNTAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2348..2425
                     /locus_tag="VYO63_001129"
                     /pseudo
     CDS             2348..2425
                     /locus_tag="VYO63_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2675..9376
                     /locus_tag="VYO63_001130"
     CDS             2675..9376
                     /locus_tag="VYO63_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1672058.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNIVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLQKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLID
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKDQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     VPKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEITVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVTQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKLIDSSE
                     ASQTNKAQLPSTGSAASQAAVAAGLTLLGLSAGLVVTKGKKED"
     gene            complement(9624..10480)
                     /locus_tag="VYO63_001131"
                     /pseudo
     CDS             complement(9624..10480)
                     /locus_tag="VYO63_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3355 a         2035 c         2295 g         2930 t
ORIGIN      
        1 tttgacaaaa accttgtgtt ttgcttagaa gcggataatc aagaacaact ctttgatcag
       61 gttgcaactt tattggaaga acgagaaatt gtcactccaa cttatcgtga agccttgatc
      121 acgcgtgaaa agtcatttcc aactggttta gatatggaat ttctaggaaa ggacttgcca
      181 aatgtagcga ttcctcatac agatattgtt cataatctag ctgagaaagt ggtggttgtt
      241 cgattagaaa aaccagtaac ttttcacaat atgatagctc ctgataagga agtagaagta
      301 tccctactct tctttattat taacaattca agttcaagtc aaacaaatat tctggctcag
      361 ttgatggact ttttcacagg aaatggacat cttgaagacc tatcaaaaat ttccgaacca
      421 gaaaaacttt atgcttacat tgctgaagca accgcttaat cttgtctatt aaaaaataaa
      481 atcggaggaa atccaaatga ttaaaattct tgctgcctgc ggtgcaggtg ttaactcaag
      541 tcaccaaatt aaaagtgctc tagaagaaga actttcaaac cgtggttatg atgttcactg
      601 tgatgcagtc atggtgaaag atgtaaacga agaccttatg aaaggttacg atatctttac
      661 accaatcgct gcaacagacc ttggttttga accaggtatc ccagttatcg aagctgggcc
      721 aatcttattc cgtatcccag caatgagcgc tccagtattt gacaatattg aagcagctat
      781 taaagaacac ggattaagct aattctaatt tgttaacatt catataacat aaaaaaggga
      841 ggaactgtta tggatgcaat ctttgaccta atcggaaagg ttttcaatcc cattttagaa
      901 atgggtggac ctgtcatcat gttaatcatt ttgacagtat tggctttact ttttggagtg
      961 aaattctcca aagcgcttga aggtggtatc aaacttgcca tcgctcttac aggtatcggt
     1021 gctatcatcg gtatgctaaa cactgctttc tcagcatcac tagcaaaatt cgttgaaaac
     1081 actggtatcc aattgagtat taccgacgtt ggttgggcac cacttgctac aatcacttgg
     1141 ggttctgctt ggacactata cttcttgctc atcatgttga ttgtcaacat agtgatgcta
     1201 gctatgaaga aaacagatac acttgatgtc gatatctttg atatctggca cttgtctatc
     1261 acaggtctct tgattaaatg gtatgctgat aacaatggtg tgagtcaagg ggtttcactc
     1321 tttattgcta cagcagctat cgtccttgtc ggtgtgttga aaattatcaa ctctgacttg
     1381 atgaaaccta catttgatga ccttcttaac gccccaagtt catcaccaat gacatcaact
     1441 cacatgaact acatgatgaa cccagttatc atggttttgg ataagatttt tgaaaaattc
     1501 ttcccaggcc ttgataaata tgactttgat gctgctaaat tgaacaagaa aatcggtttc
     1561 tggggatcta aattcttcat cggtttcatc cttggtatcg ttatcggtat tatgggaact
     1621 ccacatccaa ttgcaggtgt tgcagatgca gataaatggc gtcttgttat caaaggatgg
     1681 ttgtctcttg gtttgactgc cggtgtatct ttggaactct tctcacttat cggttcatgg
     1741 ttcatcgcag ccgtagaacc actatcacaa ggtattacaa acgttgctac taaacgtctt
     1801 caaggacgta aattcaatat cggtcttgac tggccattca tcgctggtcg tgctgaaatc
     1861 tgggcttgtg ccaacgtact tgcaccaatc atgttgattg aagcagtgct tctttcaaaa
     1921 gttggaaatg gtatcttgcc acttgcaggt atcatcgcta tgggtgttac tccagctctc
     1981 ttggttgtaa ctcgtggtaa attgctccgt atgattatct tcggaacact cttgttgcca
     2041 ctcttccttc tttcaggtac acttattgca ccatttgcaa cagaacttgc taaaggtgta
     2101 ggtgccttcc cagaaggtgt gagccaaact caattgatta ctcactctac tcttgaagga
     2161 ccaatcgaaa aacttcttgg ttggacaatt ggtaacacta caactggtga tatcaaagca
     2221 atccttggtg cagtagtctt ccttgtattc tatatcggta tctttgcttg gtacagaaaa
     2281 caaatgatca aacgtaacga agagtacgca gcaaaagcaa aataatgcgc tcctataaaa
     2341 tataaactca atgaaaatca aagagcaaac taggaagcta gccgcaggtt gctcaaaaca
     2401 cagttttgag gttgtagata agactgacga agtcagctca aaacatggtt ttgaggttgt
     2461 agatgaaact gacgaagtca gctcaaaaca tggttttgag gttgtagatg aaactgacga
     2521 agtcagtaat catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag
     2581 agtgtaaatt gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag
     2641 ctaactgcac taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa
     2701 aagagtttat agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg
     2761 tgcagtttta ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact
     2821 tattactcat actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga
     2881 taaagctttg gaaactaaaa atatagttga aaggacagaa caaagtgaac ctagttcaac
     2941 tgaggctatt gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt
     3001 gtctgctaaa ccggaagaaa aagctccaag gatagaatca caagcttcaa atcaagaaaa
     3061 accgctcaag gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga
     3121 caggaaagtg gattttaatc aaaattggta ctttaaactc aatgcaaatt ctaaggaagc
     3181 cattaaacct gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag
     3241 tatctttaac gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg
     3301 tggggaagct tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt
     3361 tcgccttact tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt
     3421 ggggcattat ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcaaaa
     3481 agatggtcgt gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg
     3541 gtattcagga agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt
     3601 tgagaaaaat gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt
     3661 tgaaactcat gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc
     3721 cgaatatcaa atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag
     3781 tcgtacctta aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc
     3841 aaaactctgg actgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta
     3901 ccgtgacggt caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg
     3961 gactccaaat gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt
     4021 gcaccacgac catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct
     4081 caaacaaatg aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga
     4141 gcaaaccttg caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac
     4201 gtggtatggt ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca
     4261 cccagaagct cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag
     4321 aggcaaaaac aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa
     4381 tggtgatgcc cactctttag caactgttaa acgtttggtt aaggttatca aggatgttga
     4441 taagactcgc tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg
     4501 gcatgagaaa attgctgatg aactcgatgc tgttggattt aactattctg aagataatta
     4561 caaagccctt agagctaagc atccaaaatg gttgatttat ggatcagaaa catcttcagc
     4621 tacccgtaca cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc
     4681 tgagcgtaat tatgaacagt cagattatgg aaatgatcgt gtgggttggg ggaaaacagc
     4741 aaccgcttca tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac
     4801 aggtacggac tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa
     4861 gagctcttac tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta
     4921 ccaaagccaa tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa
     4981 ctgggaaaac aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg
     5041 tgcttattcg aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa
     5101 gactttcaat aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa
     5161 tgaactttat cttgaatgga aagttgccta tcaaccaggt accttggaag caattgctcg
     5221 tgatgaatct ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc
     5281 agttcgtctt attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat
     5341 ctactatgaa attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg
     5401 cttccaattg catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg
     5461 tgaacgctat aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg
     5521 tgttgccatt gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga
     5581 tctcttgaaa tcgaaccaag tcactgtctt tactggtaag aaagaaggac aagagaagac
     5641 tgttttgggg acagaagtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc
     5701 taccactgtt ccgtttgtat acagtgatgg tagccgtgca gaacgtcctg taacctggtc
     5761 ttcagtagat gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga
     5821 agtagaagct cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat
     5881 tgctccaaat actgacttga attctgtaga caaatctgtt tcctatgttt tgattgatgg
     5941 aagtgttgaa gagtatgaag tggacaagtg ggagattgcc gaagaagata aagctaagtt
     6001 agcaattcca ggttctcgta ttcaagcgac cggttattta gaaggtcaac caattcatgc
     6061 aacccttgtg gtagaagaag gcaatcctgc ggcacctgca gtaccaactg taacggttgg
     6121 tggtgaggca gtaacaggtc ttactagtca aaaaccaatg caataccgca ctcttgctta
     6181 tggagctaag ttgccagaag tcacagcaag tgctaaaaat gcagctgtta cagttcttca
     6241 agcaagcgca gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggccc
     6301 tcttcaaacc tatgcaattc aattccttga agaagcgcca aaaattgctc acttgagctt
     6361 gcaagtggaa aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc
     6421 tcactatcaa gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag
     6481 tggtgaaggg gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt
     6541 cactctgaac gctgaatatg agggagctaa agaccaagtt gaactcacta tccaagccaa
     6601 tactgagaag aagattgcgc aatccatccg tcctgtaaat gtagtgacag atttgcatca
     6661 ggaaccaagt cttccagcaa cagtaacagt tgagtatgac aaaggtttcc ctaaaactca
     6721 taaagtcact tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt
     6781 actaggtaaa gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat
     6841 cgtttcagtt gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga
     6901 aagtgttcgg acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc
     6961 gattcgtcca gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg
     7021 tacgcaatta acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa
     7081 catttctgac caatggaccg gttcagaatt gccacttgcc tttgcttcag actcaaatcc
     7141 aagcgaccca gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa
     7201 tcgttggaca aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga
     7261 ttcaggtatc ttgagcaaac gctccgttga taatctaagt gtcggattcc atgaagacca
     7321 tggagttggt gtaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac
     7381 agctcctaaa aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa
     7441 ctggaaacca gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca
     7501 ctttagcttt gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa
     7561 caagcgtgga acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa
     7621 gcaaggacaa acaagaatcc aagttgacgg caaagactta gcaaacttca accctgattt
     7681 gacagactac taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag
     7741 caacaatggt ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat
     7801 cgcgaaagct gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa
     7861 gagcttactt tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg
     7921 tcaagcactt gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga
     7981 aacaaaagac ctgacagttg aatgggaaga agttccagcg gaaaatctga caaaagcagg
     8041 tcaatttact gttcgaggcc gtgtccttgg tagtaacctt gttgctgaga tcactgtacg
     8101 agtgacagac aaacttggtg agactctttc agataaccct aactatgatg aaaacagtaa
     8161 ccaggccttt gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta
     8221 tctcaatgac ggagatcatt cagaaaatcg tcgttggaca aactggtcac caacaccatc
     8281 ttctaatcca gaagtatcag cgggtgtgat tttccgtgaa aatggtaaga ttgtagaacg
     8341 gactgttaca caaggaaaag ttcagttctt tgcagatagt ggtacggatg caccatctaa
     8401 actcgtttta gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta
     8461 ccaagcctac gacgcagacc atccattcaa caatccagaa aattgggaag ctgttcctta
     8521 tcgtgcggat aaagacattg cagctggtga tgaaatcaac gtaacattta aagctatcaa
     8581 agccaaagct atgagatggc gtatggagcg taaagcagat aagagcggtg ttgcgatgat
     8641 tgagatgacc ttccttgcac caagtgaatt gcctcaagaa agcactcaat caaagattct
     8701 tgtagatgga aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta
     8761 taaaggtcaa cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt
     8821 agatagtgga gaagatagct ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca
     8881 agtcaaggaa taccgtatcc acttgactaa ggaaaaacca gtttctgaga agacagttgc
     8941 tgctgtacaa gaagatcttc caaaaatcga atttgttgaa aaagatttgg catacaagac
     9001 agttgagaaa aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa
     9061 agttggaaaa gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact
     9121 ccgtgaagtg gtagaagttc cgacagaccg catcgtcttg gttggaacca aaccagtagc
     9181 tcaagaagct aaaaaaccac aagtgtcaga aaaagcagat acaaaactaa ttgattcaag
     9241 tgaagctagt caaactaata aagcccagtt accaagtaca ggtagtgcgg caagccaagc
     9301 agcagtagca gcaggtttaa ctcttctagg tttgagtgca ggattagtag ttactaaagg
     9361 taaaaaagaa gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc
     9421 ttgtcctaaa tagaagataa agagcctgag acaaaatagt tctcaaccac aaaaaagcta
     9481 gatatttcca attgtggaac tctagctttt taattttgag tcattctctt attgtatagt
     9541 agattgaaac tagaatagta cacttttact tctaaaacat tgttagaaat cgatttgatt
     9601 gtccttatcg atttgtcctg ttcttatttc attttactat attttaggag gttattggcc
     9661 ataagtacca atcccatgtc aattctcact tgacgcttcc ctctcagatt acatctcttg
     9721 taacccaaag aagcctttat ctgcccaaag acaggttcca catcaatctt gcgttgagcg
     9781 aaaatctgtc taccttgggg agataaaagc gcctgacatt ctttagcttt caagttttga
     9841 tagcgttcgt tcatatacag tcccttttga ggggctgatt caggttcgtc ggcgtagtaa
     9901 accttgattt cttgttgaaa gtctgtctgt gttttctgat atttggtatg gtgaaaacga
     9961 taataccagc catcaggatg tgtgtagcta tcctccttgt cattatagtg ccaattcgct
    10021 aagtttttag ctgactcttt ataccctctc ttttgtttct tatcaaacat ggcatattta
    10081 atcagaaggg gagtgatttc ttcttgaaaa tagacctgta tctgttcttg aagtttggcg
    10141 gagaatttct ctgtcgtttt cttccacaca aaactatact tgttggcatt ggcttcaatc
    10201 ttagttctgt caataaacaa acaatctaag gtcactaact cttccatttt tcaatttttc
    10261 gaccagagaa aatcccttgt gaataggcaa atagaagagt agatacaagc attttagggt
    10321 gataagacgg gcgatcaaag gcatgatagg aggtgtagaa gtgacgttcc tccaaggtat
    10381 tcaccacttt ttcaatagta aaaacgagat gatcttgtgg taagaaggaa ctgatttcta
    10441 gtggtaaagt tgtttgattt gtgttatagt gaatatgcat gggatagcct ttctaaatgg
    10501 tttattgggc aattctattt taccaagact atcgtaacca tgcaaaaaag caacggcaaa
    10561 tccgttgcct ttttggatat aagagactat tgtcccagac tcttttcttt gtata
//
LOCUS       DAVLYR010000058        10411 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLYR010000058 DAVLYR010000000
VERSION     DAVLYR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.058"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            61..978
                     /locus_tag="VYO63_001132"
     CDS             61..978
                     /locus_tag="VYO63_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1672059.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            989..1879
                     /locus_tag="VYO63_001133"
     CDS             989..1879
                     /locus_tag="VYO63_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1672060.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            1908..3524
                     /locus_tag="VYO63_001134"
     CDS             1908..3524
                     /locus_tag="VYO63_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1672061.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            3534..4853
                     /locus_tag="VYO63_001135"
     CDS             3534..4853
                     /locus_tag="VYO63_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1672062.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(5064..5136)
                     /locus_tag="VYO63_001136"
     tRNA            complement(5064..5136)
                     /locus_tag="VYO63_001136"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5101..5103),aa:Lys,seq:ctt)
     gene            complement(5401..6672)
                     /locus_tag="VYO63_001137"
     CDS             complement(5401..6672)
                     /locus_tag="VYO63_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1672063.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            6942..7142
                     /locus_tag="VYO63_001138"
     CDS             6942..7142
                     /locus_tag="VYO63_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672064.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            7346..7525
                     /locus_tag="VYO63_001139"
     CDS             7346..7525
                     /locus_tag="VYO63_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672065.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            7662..7841
                     /locus_tag="VYO63_001140"
     CDS             7662..7841
                     /locus_tag="VYO63_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1672066.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            7878..8330
                     /locus_tag="VYO63_001141"
     CDS             7878..8330
                     /locus_tag="VYO63_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1672067.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            8621..9091
                     /locus_tag="VYO63_001142"
     CDS             8621..9091
                     /locus_tag="VYO63_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1672068.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            9110..10198
                     /locus_tag="VYO63_001143"
     CDS             9110..10198
                     /locus_tag="VYO63_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1672069.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            10179..10337
                     /locus_tag="VYO63_001144"
     CDS             10179..10337
                     /locus_tag="VYO63_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEV1672070.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
BASE COUNT         3206 a         1782 c         2267 g         3156 t
ORIGIN      
        1 acagcgccaa gcgctatctt ttatagaaaa aatgaaacgt ttcaaaaaaa ggaggttgct
       61 atgaatagca aagcgaagca agtttctctt tgggaaagaa tcaagaaaca aaaactcttg
      121 ttattgatga ctgtccccgg tttagtttta acctttatct ttaaatacat ccctatgtat
      181 ggggttttaa tcgcatttaa agattacaat cctttaaaag gaattttagg gagtgattgg
      241 attggttttt ctgagtttac aaaattcata tcctctccca actttggtat cttgttagcc
      301 aacacattaa aattaagtat ctatggttta ttgcttggct ttttaccacc aatcattctt
      361 gcgattatgc tcaatcaact cttgagtgaa aaagtcaaaa aacgaattca gctcatttta
      421 tacgcaccaa actttatctc agtcgttgtt attgtcggta tgattttcct cttcttttca
      481 gtgggaggac caatcaacaa ttttctttct atgtttggaa tgaaggctga cttcttgaca
      541 aatccagact tctttagacc cttatacatc tttagtggta tctggcaagg aatgggctgg
      601 gcttcaacgc tctacacggc aacattggta aatgtagatc cagccttagt agaagcagct
      661 cgactggatg gagccaatat cttccaacga atctggcaca ttgatattcc agctcttaag
      721 cctattatgg ttatccaatt tgttttagct gcaggtggaa ttatgaatgt cggatatgaa
      781 aaagcattct tgatgcagac atcgttaaat ttgccaactt ctgaaattat ctcgacatat
      841 gtctataaag ttggtcttgt atcaggagac tattcttact caacagcggt tggtttgttt
      901 aatgcagtga ttaacgtagt attgcttgtt gcagttaacc aaatcgttaa acgcatgaat
      961 aatggtgaag gaatttaagg aggaaagtat gaaaaattcg attatggata caaaatttga
     1021 tagacgtatc ttactcttaa ataaaatcat tattgtcttt atcgttttga tgactttgct
     1081 tcctttactt tatatcgtcg tagcatcctt tatggatcct aaggttctgg ttagtagagg
     1141 gattagcttt aatccagccg attggactgt agaaggttac cagcgtgtat tcagtgacca
     1201 atctattcta agaggtttta tcaattctct actatactct tttggatttg cagctttaac
     1261 agtattgcta tctgtgttta cagcttatcc tctttctaag aaagacttgg ttggacgtcg
     1321 ttggattaac tacttcttga ttgtgactat gttctttggt ggtggtttag tcccaactta
     1381 cttgctcgta aaagaattgg gaatgctcaa tactccatgg gctatcattg ttccaggtgc
     1441 tgtcaatgtt tggaatatta ttcttgctag ggcctatttc caaggattgc ctgaagaatt
     1501 agttgaagct gctgtcattg atggagcgaa tgacttgcag attttcttta aaatcatgct
     1561 tcctcttgca aaaccaatta tgtttgttct cttcctctat gcttttgtag gacagtggaa
     1621 ctcatacttt gatgcaatga tttatatcaa ggatccaaac ttggaaccat tgcaacttgt
     1681 acttcgtaaa attctcattc agagccaacc aggtcaagac atgattggag cacaagcggc
     1741 tatgaatgaa atgaaacgtt tagctgaatt gattaaatac gcaactattg tcatttccag
     1801 cttgccattg attgttatgt atccattctt ccaaaaatac tttgataaag gaattatggc
     1861 tggttcactt aaaggataaa aaaagaaaaa ataaaaggag ttttctcatg aaattcaaaa
     1921 cattctcaaa atcagcagtt ttgttgacag ctagtttagc agtacttgca gcctgtggct
     1981 caaaaaatac agcttcaagt ccagattata agttggaagg tgtaacattc ccgcttcaag
     2041 aaaagaaaac attgaagttt atgacagcca gttcaccgtt atctcctaaa gacccaaatg
     2101 aaaagttaat tttgcaacgt ttggagaagg aaactggcgt tcatattgac tggaccaact
     2161 accaatccga ctttgcagaa aaacgtaact tggatatttc tagtggtgat ttaccagatg
     2221 ctatccacaa cgacggagct tcagatgtgg acttgatgaa ctgggctaaa aaaggtgtta
     2281 ttattccagt tgaagatttg attgataaat acatgccaaa tcttaagaaa attttggatg
     2341 agaaaccaga gtacaaggcc ttgatgacag cacctgatgg gcacatttac tcattcccat
     2401 ggattgaaga gcttggagat ggtaaagagt ctattcacag cgtcaacgat atggcttgga
     2461 ttaacaaaga ttggcttaag aaacttggtc ttgaaatgcc aaaaactact gatgatttga
     2521 ttaaagtcct agaagctttc aaaaacgggg atccaaatgg aaatggagag gctgatgaaa
     2581 ttccattttc atttattagt ggtaacggaa acgaagattt taaattccta tttgctgcat
     2641 ttggtatagg ggataacgat gatcatttag tagtaggaaa tgatggcaaa gttgacttca
     2701 cagcagataa cgataactat aaagaaggtg tcaaatttat ccgtcaattg caagaaaaag
     2761 gcctgattga taaagaagct ttcgaacatg attggaatag ttacattgct aaaggtcatg
     2821 atcagaaatt tggtgtttac tttacatggg ataagaataa tgttactgga agtaacgaaa
     2881 gttatgatgt tttaccagta cttgctggac caagtggtca aaaacacgta gctcgtacaa
     2941 acggtatggg atttgcacgt gacaagatgg ttattaccag tgtaaacaaa aacctagaat
     3001 tgacagctaa atggattgat gcacaatacg ctccactcca atctgtgcaa aataactggg
     3061 gaacttacgg agatgacaaa caacaaaaca tctttgaatt ggatcaagcg tcaaatagtc
     3121 taaaacactt accactaaac ggaactgcac cagcagaact tcgtcaaaag actgaagtag
     3181 gaggaccact agctatccta gattcatact atggtaaagt aacaaccatg cctgatgatg
     3241 ccaaatggcg tttggatctt atcaaagaat attatgttcc ttacatgagc aatgacaata
     3301 actatccaag agtctttatg acacaggaag atttggacaa aattgcccat atcgaagcag
     3361 atatgaatga ctatatctac cgtaaacgtg ctgaatggat tgtaaatggc aatattgata
     3421 ctgagtggga tgattacaag aaagaacttg aaaaatacgg actttctgat tacctcgcta
     3481 ttaaacaaaa atactacgac caataccaag caaacaaaaa ctagaggttg attatgggag
     3541 ataagaaata cacagtagaa aaagccaatc gttttatagc agaaaataaa catctcgtta
     3601 atactcaata taagcctgaa gaacattttt cagctgagat tggttggatc aatgatccaa
     3661 atggatttgt ctattttcgt ggagaatacc atctctttta tcaattctat ccatatgata
     3721 gtgtttgggg gcctatgcac tggggacatg ctaaaagtaa ggacttggtg acttgggagc
     3781 acttgccagt ggcacttgct cctgaccaag attatgaccg aaatggttgt ttctcaggct
     3841 ctgccattgt caaggatgat cgcctctggc tcatgtacac tggacatatc gaagaagaaa
     3901 ccggtgtccg ccaagtgcaa aatatggcat tttcagatga cgggattcac tttgaaaaga
     3961 tttcccaaaa tccagttgca actggatcag acttgccaga tgagttgatt gctgctgatt
     4021 tccgtgatcc aaaactcttt gaaaaagatg gacgctatta ctccgtagta gctgccaaac
     4081 ataaggataa tgtgggctgt atcgttctac tagggtccga taacctagta gaatggcagt
     4141 tcgaatccat ctttttaaaa ggggtagaac accaaggctt tatgtgggaa tgcccagatt
     4201 acttcgagtt agatgggaaa gattgcctta ttatgtcacc catgcgttat cagcgtgagg
     4261 gagactcata tcataacatc aactcatcgc ttttgttcac gggtaaggta gattggggag
     4321 aaaaatgttt tatcccagaa tcagttcaag aaattgatca tggccaagac ttctatgcgc
     4381 ctcaaacatt gttggacgat caaaatcgtc gtatcctgat tgcttggatg cagacatggg
     4441 ggcgtaccct tccaacccat gaccaagaac acaaatgggc atgtgccatg actttaccta
     4501 gaattttaag attggaagat ggcaaactaa gacaattccc tgttaaaaaa ggccaatatc
     4561 aaatccaaat agataaagat tgtcattacc acttaggaaa tgatatagat tatcttgaat
     4621 ttggttatga tagtaatgcg cagcaagttt acattgatcg tagccatctt attcaaaaaa
     4681 ttctaggtga agaagaacag gacactagtc gacggtatgt agatattgaa gctaaagaat
     4741 tggaagttgt tctagataaa aattccatcg agatttttgt caatcaaggt gaagcaagct
     4801 tgactgcaac ttattactta acggtgccag ctgagctatc acgaattgat taaaaattaa
     4861 gttatttctc ctaaagaaaa agttctcttt ctaaaatagt ggaaagagga ctttttgtgt
     4921 tttgggtata taagcttagt ttatggtatt tgtaaaattg gtgttggatt atgatttaag
     4981 ctagttttct aaagaatttg aaaaaaattt tatttaagca aaaaaaaacc cttggttcca
     5041 aggcttttcc tgttgtattt atatgccccc tacagggatc gaacctgtga cccacggatt
     5101 aagagtccgc tgctctgcca gctgagctaa ggaggcaaag aaaaaagctg tattggtgcc
     5161 gaaacttcac ggtttgtatt gaacccgcgc aattaagcag gtgggcaact cgctctaact
     5221 gaagctgttt ccgtgtgaga cggcctacat gctgttagaa gacttttgtt tccctaataa
     5281 tacaaaaaat agtcggtcaa cacttaagtg tgaagtcgta caccacagcg tttctatact
     5341 tatatgatac cactttttca aaaaaaatca agaggaaagt gcaatttttt gaaaaggatt
     5401 tcagattttt cgcaaacggt cgatagcttc ctttctttga gccaaagccc gttcatattt
     5461 accagtatct tctgcttgga aatagtgatg attacgaatt ttttctggca gatagtcttg
     5521 cttgacccaa tttccaggat agttgtgtgg atagagatag tcttgggcat tccccagttc
     5581 cttgcttcca ctgtagtgcc catcacgtag gtgtcgcgga ataggcaagt gccctgatgt
     5641 tttgaggtca gcaagtgcct tatccatagc tacataggct gagttggatt ttggagaaag
     5701 ggccaaatca atcacgacat tggcaatgag aatgcgggct tctgggaacc caatcttctg
     5761 ggcagcatcc agagcagtca cggtatgaat ctgggcctca ggattggcta agccgatatc
     5821 ttcataggcg ataacagtca agcgacgagc gagactaggc agatccccag cctcaatcaa
     5881 gcgggcagta tagtggagac tggcatccac atctgagcca cgaatagact tttgcagggc
     5941 agatagaaca tcatagtgac cgtctccatc cttgtccata gtgatgtagc ttctctgaag
     6001 actattttcc atgatgtcta aggtgatgtg gcgaatgccc tcgtcattct caggggtaga
     6061 gagaacagct aagtccagtg agttaaaggc agagcgaagg tctccgtttg tagaggttgc
     6121 gatgaaatcc agcgcatcct catctagttc tattggaaaa tcaaaaccac gttcagggtt
     6181 acttagagct atctgcaggg cctctttgac gtcttggtta gacagaggtt ccaactcgaa
     6241 aatttgaact cggctacgaa tggcaggagt gacagagaag aaaggatttt cagtcgttgc
     6301 tccaatcatg atgaccagtc cactttccaa gagaggcaag aggaagtctt gcttggtctt
     6361 atctagtcga tgaatttcgt ctagcaatag gacgagacca ccagaaaatt tagcttcttc
     6421 cgagatttct tgcagtcgct ttttactatc aactgtcgca ttgaaagttc gaaaggcata
     6481 cttggtcgtt ccagcgatgg cagaggcaat actggttttg ccgattccag gagggccata
     6541 tagaatcatg gaggacaggc ggttggcttc caccatgcgg cggatgattt ttccaggtcc
     6601 gaccagatgc tcctgaccga tgacctggtc gatggtttta gggcgcatgc gaagcgcgag
     6661 attgtctggc atagcagtcc tttctagcgt ggatttgagt agaatgactc atctactata
     6721 tcttactatg tccattctag cctattagtt gtgatatttc aatggttttg taataatcag
     6781 ggagtgagat agaatctttt ttactattta atttaatgag gagaggtatc agcatgagag
     6841 atttgatttt ccttaaagct caaaatagga tagttgagcc taaagaagtg gaagtgctga
     6901 cttgagaaat aatattgctg aatgtcattg tatgaactat aatgaaaaga gaggtgataa
     6961 gtaatggaaa tgatggacca agtcaggaaa ttttgatttt tacaaaacaa attcgtcatt
     7021 ggattcttag tgatcaagtt atttcaggga aaagaaaact tttcttccgg gaagatactc
     7081 caaaagagat tttagatttg tacgaaaaca ttaaatctaa acttgatttt gcttaccaat
     7141 aagtttattc taataatggg ttgaaaaaat atgaaaaata gaataattga tgtttttgaa
     7201 gtagtaaatc gcatccttgt aataactgtt gaaaatcctg atttcgaaga cttgagagtt
     7261 aatcatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgtacttgag
     7321 tacggtaagg cgacgttgac gtggtttgaa tttgattttc gaagagtatc agtttgtaaa
     7381 aattggagat aaaaaatata gagtacgcag tgttcctatg attcattcaa ctccgcctca
     7441 atctgtctta gatcgagata cttttacaat tgactataca gatgatgaat tgctcgataa
     7501 agaagcagtt tttactactc attagaaaaa taggtctgaa taaattggct tcccttagaa
     7561 ccacatctag gggcttttat gtataataga ataataagta tcactccttt acaatacgtg
     7621 taacacgtgt tataatggtg ttgtcaggag gtaaaagcgc tatgcctatg acacaaaaag
     7681 agatggttaa actccttacg gcccatggtt ggataaagac tagaggcggt aaaggctccc
     7741 atattaaaat ggagaagcaa ggggaaagac ttatcacaat ccctcatggt gagctgaata
     7801 agtacactga aagagggata agaaagcaag ctgggttgta aaattcagcc cctgtttttt
     7861 gatatggagg tagtgaaatg ttagttacgt atccagcttt attttattat gacgacacag
     7921 acggaacaga agcgacttat tttgtccatt tcccagattt tgaatactca gctacacaag
     7981 gagaggggat ttctgaggct ttggctatgg ggtcggagtg gctagggata actgttgcag
     8041 atttgataga gagtgatgga gagttacctc agccatcaga tatcaatagc ttgtctttaa
     8101 ttgataatga tccttttaaa gatgatgaag atttcgtgtc aacctatgac cttgataaat
     8161 cttttatttc tatggtatct gttgatgtat cagaatacct aggaagtcag gaacccataa
     8221 aaaagacttt gaccatacca aaatgggcag ataagttggg acgagaaatg ggacttaact
     8281 tttctcagac tttgacagac gctattgcag ataagaaagt tcaagcctaa gattaagcgt
     8341 ggtatcgtgg taatagtcaa taaaaaagca cgtccacttg tgctagttac tcgcctattg
     8401 attttataga tttattagcc ctttcgcggg ctttttatat tgatttttat gagaaatgaa
     8461 gaaatcagac ttttaagaaa atcactgata ccaagggttt aaatgaggta atatggtata
     8521 attaggacat aaaataattt tgtggtaaga tggtagtatc tattttagca tatttccgag
     8581 caatcggggc gattaaagag tcgcatagaa agaggacaaa atggcaacat acggattttt
     8641 agatgtttta gaggaagagt tggacaagaa ctttcccttt gactttgaga ttagctggga
     8701 caaacgcaac catgcggtag aagtgagttt tctattggaa gcgcaaaatg ctgcaggtgt
     8761 ggagatggtg gatgaagacg gagaggtttt atcagatgac attctttttg aggaagcggt
     8821 ccttttctac aatcctgcca agtcaacagt caatgaggaa gactatttga ctgttattcc
     8881 ttacctgcct aaaaaaggat tttcccgtga atttttagct tattttgcgc ttttccttaa
     8941 agatactgct gaggttgggc tagatgtcct catggacttt ttggaagacc cagaagcaga
     9001 ggaatttgtc atggaatgga accaagaagt ctttgaagaa ggaaaagttg gcttggaaga
     9061 gggagaattt tatccttatc caagatacta ggagttggtt ggaggtgtta tgaagaaaat
     9121 tgggacttat ttggtttatg tgctagcttt tgtctttatt atgctagctt ttgcttgtgg
     9181 aacaatcgca tttgcagagt tggggtattc cgcagtttta gcctttactt ttggttatgc
     9241 cttcgctctt ctaagcatgt atttaatctt tattcttcat gagctgggtc atgctttttg
     9301 tggctacttg acaggctatc ggctggtggc ttttggatta ggacatttta ttttgaccaa
     9361 aaagtcaggc aggtttcatc ttagtagaac agccattctg aaaaatgttg gtgctcaata
     9421 cattggttta aaagaggatg aaagcgatca aagaatcatc ctgatgcttt caggaggctt
     9481 gatggttcat ctcggcttga tattattggc gatagtgttt ggatttttga caagaagctg
     9541 gtattttgca gggacttgga tttttcttaa tttatctttc ttcctaaata acattttgcc
     9601 agtcgacatc accgatggag caaaaatttg ggaattacta caacaccctg aaaataccaa
     9661 atacgcctac ctggtattga gacattctgc tcaaactttg ctggctcctc aagaatatga
     9721 tttgaaagac tttgttcaag cagttcctga ggatgcacga gggagttttg cggatggtgt
     9781 attaggtatg caaggtgaag tttccatttt ggaagggaag gaagaagtag ctaaacagca
     9841 gtttcaagca ttacttgaga ggacaactac tccgatgatg cagacagttg cccaactatc
     9901 cctgcttcat atcgccttgt tggaaggaga ttttgagcaa gcggagcaat atgctagtat
     9961 tcgacaggtc aaatcgtttc tgtctctcaa actgtccaat cttcaagtga tacaagcttg
    10021 gtatcaattt aaggtaaaga aagatgtaga tcgaactcgt aaggctctca agattgctag
    10081 acagaaaatg gatagtagtc gaatgttgag ggacgagaaa cgctattatg aaaacttgtt
    10141 agcagagctg gaaaaggaaa tgttagaagg aactgaagat ggaactagaa atttctgatt
    10201 tcccaggctg taagattgct ttgttttgtg gggataagct cttgactatc ttacgtgatg
    10261 ataaggcaag tattccctgg gccaatatgt gggaactgcc aggtggtgga aagagtgtca
    10321 gggaaaatct gatatagttt tgtattaaaa gaatatcctt tcaaggttat tcttttttaa
    10381 ttttgtccca aaataattga ctttgtcccg a
//
LOCUS       DAVLYR010000059        10386 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLYR010000059 DAVLYR010000000
VERSION     DAVLYR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.059"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..630)
                     /locus_tag="VYO63_001145"
     CDS             complement(<1..630)
                     /locus_tag="VYO63_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1672071.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYL"
     gene            complement(632..1459)
                     /locus_tag="VYO63_001146"
     CDS             complement(632..1459)
                     /locus_tag="VYO63_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1672072.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKVRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDIIQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1481..2798)
                     /locus_tag="VYO63_001147"
                     /pseudo
     CDS             complement(1481..2798)
                     /locus_tag="VYO63_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189734.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            3056..3667
                     /locus_tag="VYO63_001148"
     CDS             3056..3667
                     /locus_tag="VYO63_001148"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1672073.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3712..4440
                     /gene="rlmB"
                     /locus_tag="VYO63_001149"
     CDS             3712..4440
                     /gene="rlmB"
                     /locus_tag="VYO63_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1672074.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKNLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENSLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4479..5390)
                     /gene="trxB"
                     /locus_tag="VYO63_001150"
     CDS             complement(4479..5390)
                     /gene="trxB"
                     /locus_tag="VYO63_001150"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1672075.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5456..5680)
                     /locus_tag="VYO63_001151"
     CDS             complement(5456..5680)
                     /locus_tag="VYO63_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1672076.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5758..6501)
                     /locus_tag="VYO63_001152"
     CDS             complement(5758..6501)
                     /locus_tag="VYO63_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1672077.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6501..7301)
                     /locus_tag="VYO63_001153"
     CDS             complement(6501..7301)
                     /locus_tag="VYO63_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1672078.1"
                     /translation="MNVTTILASDWYQNLMQLIPNGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7459..7815)
                     /locus_tag="VYO63_001154"
     CDS             complement(7459..7815)
                     /locus_tag="VYO63_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1672079.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7809..8339)
                     /locus_tag="VYO63_001155"
     CDS             complement(7809..8339)
                     /locus_tag="VYO63_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1672080.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(8339..8833)
                     /locus_tag="VYO63_001156"
     CDS             complement(8339..8833)
                     /locus_tag="VYO63_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672081.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            8968..9414
                     /locus_tag="VYO63_001157"
     CDS             8968..9414
                     /locus_tag="VYO63_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1672082.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9411..10058
                     /locus_tag="VYO63_001158"
     CDS             9411..10058
                     /locus_tag="VYO63_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1672083.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILTASVLQYIAIVYYM"
BASE COUNT         3074 a         2398 c         1815 g         3099 t
ORIGIN      
        1 aagatagtct ttcaaggctt ttgacaaagc ctgataacca gcaatactga ggtgcagtcc
       61 atcagttgta tattcttttt tgagttggcc tgcttggtct gtcaaacaat caaatactgg
      121 cacaaattcc acctgcatat aggcagatgc aagctcttga taggcttgat tccagttctg
      181 aattttttca ttcgagcgga tatagactgc ctgctggtac tcctctccct cattgacagg
      241 caaaatggaa agcaatttaa tctctgtcaa tggataatcg cgagcaacgg attgaatgat
      301 agcttcgaga ttattgagag cctcattcac aggaacatcc tttccgatat catttgtccc
      361 aatcagaaga aaaattttat ctactgctcc accatataga tgagcatcaa gattctctag
      421 taacagtcct gtctgatagc cacgaattcc tcgattgaca atcgtctttg aagtcccaaa
      481 tagctcctgt agaggataat actcgacaat ggaatcccca ataaaaagaa tgtttggttc
      541 tacaacagaa atgtgattta ggtgacgata cttagtttga attttttctt gttcctttag
      601 gagccaattt tctaataact gtactgccac ttcattctcc tttttctaac cagttttcca
      661 agacttgata aactcctcct tggctgttgg ctggtgctag agcagtcgcc acagctttgg
      721 ctttctcatc agcattttcc atcgcatagg caattccagc catttcaagc atttcaacat
      781 cattttcact atcaccaaaa gccatgattt cttgggattt caagtcccag cgcttgagta
      841 attcctccaa gccccatgct ttatgaatcc cagcttggat gatatcaatg caaccatagc
      901 cactggatac agctcggaca cggccatcaa agagagcatt gatttcttcc aaaaccgaac
      961 tcaaacgttc ctcaccaaca accatgctca tcttgagcac accaccaaag aggtcagatg
     1021 ttaattcatc cacaaattgc atccgttggt agaatttttc aatcatttct ggagtcataa
     1081 aactttcaag atctgtaaaa atcgtacctt ccttgacaaa atcacccttc atccccgtta
     1141 caacaaactg gtcctgacac gctcgaacct tgaaatgagt caaagccttg ttgacaatgg
     1201 cgtcatccca tgtctgagcc tgaatcaatt cattgttttc aaaaatacga gcgccattag
     1261 caacaaccag aaccactcga tccaccaagg gactcagtag ttgtctcatg cggtgaattt
     1321 cattgcccgt cgcaatgaca aaacgaatgc ccctttgatc caactgatct aaaatctttt
     1381 ccaatcgtgg gagatcaagc tgacctctag catccagcaa ggtcccatcc atatctgtcg
     1441 caattacctt aatcatcatt tactttcctc tggattcaag ctaataccac ttcaatccca
     1501 catgttcatt catttcttta taggctgtat caattcgttc cttagtcgct tcatctaact
     1561 ggtcacgatg actgccatcc tctataaaat cttccaaaat ataagtttgg taatgataca
     1621 agggatatcc ttctggtgaa aaggttccct ttggtgttcg attgacccaa gtaggatgat
     1681 ctttagctgt tccgatagtt gtttttccat cattcttctt gatggtgaca tccatgagaa
     1741 caccacgttc agtccactta gcattctctt catctcccat agattcaatt cgttgattgg
     1801 aaatgaagtt ccccattgaa tagataatga gtttcttatc tccatctttt tcaaccgttt
     1861 cagatggttc aacaacgtga ggatgccctc caaagataat atccgctccc caatcgatca
     1921 tcttgtgata aagagctttt tgttcttcag ttggttccaa tcgatactca acacccatct
     1981 gaggcatgat aatggtgata tctgcttcct tctctgcccg ttcaacttca gccttcatct
     2041 tatcttcgtt taaatctaaa agataacgat tatagtcttc ctgagaaata tactgctcaa
     2101 ttccattgaa accataggaa taggctgaca atgcaacctt gataccattc acttccttaa
     2161 tgaccagcgg aacctgatca cgtggttcgt gcgtataaac tccgattgga gtgattccag
     2221 ctttctcaat aatatcggcc gttgaaataa ctccctcaat ttgcgaatcc aaaatatgat
     2281 tatgagctaa atccagcaca tgataacctg cctccttaat agcatccata acttcagcag
     2341 gagcattaaa gagaggataa cccgctaaat aatgatcctt attaatggtt tcttcaaaat
     2401 caccaatagc taaatctgct tgcttgaacc aaggagtcac atactcaaaa ttttcatgaa
     2461 agtcataggt accgtcttct tttttagctg aaaagaaaag tccatcgtgg tagagcaaat
     2521 ccccgtgggc cataattctg gcagtttttt cctcttccta ctcctgagac ttttgcttag
     2581 ttccctcttg agaaatagta tctcgttttt gactagtaaa gagattcctt ggaagctttc
     2641 aaacaacaag accaagccca ttgataaacc aactgctagc aagagtatcg ccacaaatcc
     2701 cttattgctc cacttgcgat aactcctaaa aagtttacca agcccttcat aaaacgaaaa
     2761 gctaaaccac cctgatttcg attttgtctt ctttgcatct tcattctccc tactttctta
     2821 tgcaagcctt ttctttttat tatatcacag ataagtattt ctttcacaat tgaattgaac
     2881 ttcccatcta ttttctataa atcctaaatg ccataatgct ttcaattctt gtcattttgt
     2941 gatatcatgt agaggtaatg aaaggaaatg aactaatcca cagtggctta ttccaagtat
     3001 accacttggg ctttggcagt agttaactgc actaaacata atataaggag agaaaatgtc
     3061 tgcaatagaa cgtattacaa aagctgctca cttaattgat atgaacgata ttatccgtga
     3121 agggaatcct actctacgcg cgattgctga ggaagtcact ttccccctat ctgaccagga
     3181 aatcatccta ggcgaaaaga tgatgcaatt ccttaaacat tcccaagatc ctgtcatggc
     3241 tgaaaaaatg ggactccgcg gtggtgttgg actggctgct ccccagttag atatctcaaa
     3301 acgcattatc gctgttttgg tacctaatat tgttgaagaa ggcgaaactc cacaggaagc
     3361 ctacgatttg gaaggcatta tgtacaatcc aaaaatcgtc tctcactctg ttcaagatgc
     3421 tgctcttggc gaaggagaag gttgcctgtc tgttgaccgt aacgtgcctg gctatgttgt
     3481 tcgccatgcc cgcgttactg ttgactactt tgacaaagat ggagaaaaac accgtatcaa
     3541 actcaaaggc tacaactcca ttgttgttca gcatgaaatt gaccacatta acggtatcat
     3601 gttttacgat cgcatcaatg aaaaagaccc atttgcagtt aaagatggtt tactgattct
     3661 tgaataaaga aaatcccgtt gcaagacggg gttttgtgtt ataatagagg catgaaaaca
     3721 aatgatattg tctatggtgt ccacgccgtt accgaagccc tccttgcaaa tacaggaaac
     3781 aaactctacc tccaagaaga tctccgaggt aagaatgttg agaaagtcaa ggaactagct
     3841 acagaaaaga aggtgtccat ttcttggaca tcaaaaaaaa atctctctga gatgactgaa
     3901 ggtgctgttc atcaaggttt tgttctacga gtgtctgaat ttgcctatag cgagctagat
     3961 tacatccttg caaaaacacg ccaagaagaa aattcacttc tattgattct agatggtcta
     4021 accgatcccc ataatctggg ttctatcttg cgaacagccg atgcgaccaa tgtttcaggt
     4081 gtcatcattc ccaagcaccg tgctgtcgga gtaactcctg tcgttgccaa aacagccaca
     4141 ggtgctattg aacacgttcc aattgcccga gtaaccaacc tcagtcaaac cttagataaa
     4201 ctcaaggatg aaggtttctg gacctttgga acggatatga acggtactcc ttgccacaag
     4261 tggaatacaa aagggaaaat cgccctcatc attggaaatg aaggaaaagg tatctctagc
     4321 aacataaaaa aacaggtcga tgaaatgatt accattccga tgaatggaca tgttcaaagc
     4381 cttaatgcca gtgttgctgc ggccattctc atgtacgaag ttttccgaaa tagactataa
     4441 aaaagtttcc agtcatctga ttggaaactt ttttatgatt aactatgttc tgtaatgaat
     4501 ttataggctt cttgaccagc gatagctcca tctccaactg ctgttgttac ttggcgaagg
     4561 tctttcaagc gaacatctcc aactgcaaag ataccgtcga ctgcagtctt catgtggcta
     4621 tctgtcacaa tccagcctgc ctgatcttgg atattcaatt ctttaacaaa atcgctaaga
     4681 gggtccaaac caacatagat aaagacacca ccgaaggctt gctctgtcac ttgacctgtt
     4741 ttcacatttt caaaaacgac agattctact ctgttttcac ccttgatttt ccttactaca
     4801 gaatcccata taaagctgat tttctcattg gcaaaggcgc gatcttgtaa aaccttttgg
     4861 gcacgaagtt ggtcacgacg gtgaacaatg gtaacagtct tagcaaaacg agtcaagaag
     4921 agggcttctt caacagctga atctccacca ccaactacca ataaatcttg gtcacggaag
     4981 aaagcaccat cacacacagc acagtaagaa acaccacgac tgttcagttc ttcttctcca
     5041 ggcactccca aaggacggtg tttagaacca gttgctacga taactgtacg tgtttcatat
     5101 gtttggtcat cagtcatcac tttcttaaaa tcaccatggt cttcgacatt ttcaacataa
     5161 ccataaatgt gctcaacacc aagattttca agtggttcaa acatcttttc agccaattca
     5221 ggtccactaa tattagcgta tcctgggtaa ttttcgatat cagatgtatt attcatctga
     5281 ccacctggca gaccaccttc aatcaaagct acttttagat tgcttcgagc agcatacaag
     5341 gccgcagtca tccctgcagg tccagcaccg ataataatag tatcgtacat atagattcct
     5401 tctttcttgt tgtaactatc tttattctaa ctctttcttg tcaatcacgc aaggattatg
     5461 ccttgaaaac aagaattaaa ctcataaccg caaaggataa tactgggaca accaagactc
     5521 caatcataat catatcatag agatgggctt ggcgagcctt ggctgtctta tcaactcccg
     5581 acatggctct cagaccaatc caaatcccta aaaaaatcag aacaaggatg gtggtcaaga
     5641 tcaaactctc gaaatataaa gaaaatagtt gcagtagcat gatttctctc atttctatct
     5701 tttttaaaga gtaaactcag ctagtccaac taactgagtt ttcctttatc tattatatca
     5761 aatataagtc cgtttgtaac tagcgaagaa ttcttttgtc cgctcttctt taggggtgtg
     5821 gataatctca tccggagttc cagactcgat gattttcccc ttatctaaga agagaatctt
     5881 atccgcaact tgggctacaa aggacatgtc atgactgacc aaaatcatgg tctgacctga
     5941 cttagcagca tctgcaatag acttttctac ttcaccgacc aattctgggt caagggctga
     6001 agttggttcg tctaagagca aaacatctgg tttcatagca agcgcacgcg ctagggcaac
     6061 ccgttgcttc tgtccacctg ataaatggcg aggataatgg ttttcacggt ccgaaagccc
     6121 aaccttagcc aactcttcct tggcaatctt agtcgcttct tggtcagata atttcttgac
     6181 aacaaccaag ccttctttca cattatcaag tgctgttcgg cgttcaaaca aattaaactg
     6241 ttggaaaacc atagacaact tacggcgtag ggcaaggatt tcttcttgag tgattttaga
     6301 aaaatcaact gaaaaaccat caatctgaat agagccactg tcaggtgttt caagataatt
     6361 gagactgcga agaaaggttg attttccagc tcctgaagaa ccaatcaagg ctacaacttc
     6421 ccctttttga atatccaagt ccagatgatc caagacagtc tgtcctgaaa aggatttgct
     6481 taaattcgaa atcttaatca ttaacgaagg tctcctttca catctgtttg cactgtatca
     6541 ggtgcagaaa tagccatttt tctctcgatg aaacgaccga gactttcaat tccgatattg
     6601 actacccaat aaacaagggc aacggagatg aagcgttcaa aatagcgata atcagctcca
     6661 cctagaatct gagcttgggc aaagacttcc acaacacccg cactaaaagc tagagatgtt
     6721 cccttggtca aaccgatgag ggaattaatc aaggttggag tagctaccac cgctgcatta
     6781 ggaataatca ctcgtcgata aacttgcgct cgggtcatac ccagactgcg tgccgcctca
     6841 atctcaccag gattaactga gagaatggct gcacgaatgg tttcactagc ataagctgcc
     6901 tcattaaagg caaaggcgac aatcgcaaaa gctgcagctg gaatcgcatt gatattgaga
     6961 ccagttcccc attgctgatt gagggctttc aaagccaaag ggattccgta gtaggtcaac
     7021 atgagttgca ccaaaatcgg tgtccctttt aagaaactaa caaagaaggc ctgcaaggga
     7081 tataaaatct tgacacgatt gatcttcaca atggcaaaaa gaagcgccaa aaccaagcca
     7141 aaaagggcac caccaattgt caacataatt gttgttggaa gttgttggac aattctaggg
     7201 attccatcaa agaccgaacg taggctaaac agcttgccat tcggaatcaa ttgcatcaag
     7261 ttttggtacc aatctgatgc taaaatcgtt gtaacattca taaaaacatc ctcttctgat
     7321 aatgattcat tctaccatgc ttctgccgtc aatgaaatta tttgctacgg tcagatacag
     7381 actttgtcta cctactagtt tatcatactt tgaaacaggg aagttatata ttttatctat
     7441 caaagagata gataaaaact atttcagtcc cagttcttca taagattttc gataaccgat
     7501 ttgcttaacg gttccatttt ctactaaaat gggccgtttt aacaacatac cgtcacttgc
     7561 tagcaactca gccgcttctt ggtttgacaa acttcctacc ttatctttta gccctaattc
     7621 acggtatttg ataccactgg tgttgaaaaa ttgcttcaat tcaaatcctg aggtttctag
     7681 ccaattcaaa atgacttctt ggctaggtgt ttcttccacg atatggacgg ctttatagtc
     7741 cacacctaat tgatttaatt cttgttttgc ttttttacaa gttgaacatt tggggtattc
     7801 gataaattct aacatgtgct tctccttcta ttgcttctat ctttaaaatc tactccttca
     7861 tgctccaaga gccaagcttt cttttccact cctgcagcat aacctgtcag acgcttgcct
     7921 gctcccaaca cacgatgaca aggtactagg atagaccaag gattgcgtcc cactgctcca
     7981 ccaattgctt gagcagaagc cacttgcagg tcttgagcaa tttgtccata ggtcactgtc
     8041 tgaccataag gaatgccctg taaatagtcc caaactctct tttcaaaatt cgttccgatt
     8101 ggcgccaaga gcaagttgga taaatcctga ggcttgcctt taaagtaatc atctaagcaa
     8161 gcaataactg ggtctaaaat aggatgacta acaacttctt ctatcgtttc atctcctagt
     8221 cccctctcaa aatgcttctg ctcctgaacc caaattccat acaaataatg gtcatcagct
     8281 acaagtgata gaataccaat tggtgaggag tagagtattt ttacatactt cttttgcatt
     8341 atttctttaa tttttgatag gccaagcgtt cgccatctac tggcacctta ccaacttgtt
     8401 taaaaccaag tttttcaaaa atatgttgca taaccttgtt ttcagcatgc gtatctgagc
     8461 gaaaatcaag ataatcaaaa ccttcaatca agccctctaa gaaggtttga gcaactcctt
     8521 ttccctgcac atctgctgcc acagcaatac ggtgaaagac tagatactct gactctccag
     8581 cttgccagtt tccctcataa atagcttcat aggcctcctc tggactcttg gtcacagcag
     8641 cataggctag cagttctccc tcttccaagg ctacgtaggc ttgccctgag atgatatcat
     8701 cgataataac atctgcattt ggatagccat tttgccactg gtcactacca gcatctgcta
     8761 aacatttttt ggcatcctcc atcacctgca tgatggcatc tacttcattt ggaaaagcta
     8821 aacgaatttc catctttttt cctcatttct gactagtttc tcccatcata gcataattta
     8881 agtatttcta caagcagtta aaacttgact ttgtgttttt gttattttat aatctagtta
     8941 tcaaaactat ataaaaagga gttgaaaatg aaaactacct tttcctaccc aaaatgggca
     9001 gaaattccaa acattgacct ctatctggac caggttttgc tctatgtcaa tcaggtctgc
     9061 gcccctatct ctcctaataa agacaagggc ctaacagcat ctatggtcaa caattatgtg
     9121 aaaaatggtt acctgacaaa gcctgacaaa aaaaaatacc aacgccaaca gattgcccgt
     9181 ttgattgcta tcacaaccct caagtctgta ttttctattc aagaaatagc ccagacactt
     9241 aatactctac aaactcaagc aagttcagac caactctacg atgcttttgt ggactacatg
     9301 aaccaaggaa ttgacccagc taaccctatt atccaaacca gctgccaaac cgttaaactc
     9361 tatcatcaaa ctctagactt aatcgatcat actcaagagg aggtaatcca atgaacacta
     9421 gtcttaaact cagcaaacaa ctcagttttg gagaggagat tgctaatagc gtgacccatg
     9481 ctgtgggtgc agtcatcatg cttatcttgc tgcctatttc atccatctat agttatgaag
     9541 cacacggatt tttatcctct atcggcgttt ccattttcgt catcagtctc tttctcatgt
     9601 tcctatcatc caccatttat cactctatgg cctatggttc gacccacaaa tatgttttgc
     9661 gaatcattga ccattctatg atttacgttg ccattgccgg ctcatacacg cccgttgtct
     9721 tgaccttgat gaataactgg tttggctatc tgattattgt catccaatgg ggaacgacca
     9781 tctttggtat tctctataaa atctttgcta aaaaggtcaa tgagaaattt agccttgctc
     9841 tttacctgat tatgggctgg ttggttctgg ctatcattcc tgccattatc agtcaaacga
     9901 cacccgtttt ctggagtctc atggtaactg gcggactctg ttatacagtt ggagctggat
     9961 tttatgccaa gaaaaaacct tatttccaca tgatttggca tctctttatc ctaactgcgt
    10021 ccgtacttca atacatcgct attgtttatt acatgtaaaa ctctcagtag gcagagcctg
    10081 cttagagttt tacaaacgaa tttcccaaag aggaagcaat atgaaagcag tcattgcttg
    10141 tgttcataaa attctcagaa tcatttacaa actactctcc accaagtaga cttatcaaaa
    10201 agaaaaggcg ctaggactga agaaacagtt ctaacgccca aattaaaaaa atttcaacta
    10261 cagtatagca caggaaatga ttttttgcgc ttttttactg ttttttcaaa taaaaaaaga
    10321 caaagtttta tccttgtctt ttagttttag tattgctttg gtttcaatgt caataagtgt
    10381 gttctt
//
LOCUS       DAVLYR010000060        10362 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLYR010000060 DAVLYR010000000
VERSION     DAVLYR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.060"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(308..700)
                     /gene="rpsI"
                     /locus_tag="VYO63_001159"
     CDS             complement(308..700)
                     /gene="rpsI"
                     /locus_tag="VYO63_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1672084.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(719..1165)
                     /gene="rplM"
                     /locus_tag="VYO63_001160"
     CDS             complement(719..1165)
                     /gene="rplM"
                     /locus_tag="VYO63_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1672085.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISELI"
     gene            1522..1839
                     /locus_tag="VYO63_001161"
     CDS             1522..1839
                     /locus_tag="VYO63_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1672086.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1990..2802)
                     /gene="folK"
                     /locus_tag="VYO63_001162"
     CDS             complement(1990..2802)
                     /gene="folK"
                     /locus_tag="VYO63_001162"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1672087.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2845..3399)
                     /gene="folE"
                     /locus_tag="VYO63_001163"
     CDS             complement(2845..3399)
                     /gene="folE"
                     /locus_tag="VYO63_001163"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1672088.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3380..4702)
                     /locus_tag="VYO63_001164"
     CDS             complement(3380..4702)
                     /locus_tag="VYO63_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1672089.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLESIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGCFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADH
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKARNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4704..5648)
                     /gene="folP"
                     /locus_tag="VYO63_001165"
     CDS             complement(4704..5648)
                     /gene="folP"
                     /locus_tag="VYO63_001165"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1672090.1"
                     /translation="MSSKANHAKTAICGIINVTPDSFSDGGQFFALEQALQQARKLIA
                     EGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDTWKSQVAEAAL
                     AAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLIFPHFGFGQTF
                     TEKELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGLTKKENLLLLR
                     DLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTASAHVTSIAARQ
                     GVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5773..6480)
                     /locus_tag="VYO63_001166"
     CDS             complement(5773..6480)
                     /locus_tag="VYO63_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1672091.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLMVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6533..7951)
                     /locus_tag="VYO63_001167"
     CDS             complement(6533..7951)
                     /locus_tag="VYO63_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1672092.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTKVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8141..8953
                     /locus_tag="VYO63_001168"
     CDS             8141..8953
                     /locus_tag="VYO63_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1672093.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9292..10311
                     /gene="adhP"
                     /locus_tag="VYO63_001169"
     CDS             9292..10311
                     /gene="adhP"
                     /locus_tag="VYO63_001169"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1672094.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3049 a         2339 c         2020 g         2954 t
ORIGIN      
        1 agtagattga aactagaata gtacacctct acttctaaaa cattgttaga attcgatttg
       61 attatcctcg gtttgtccta ttcttatttc accttactat aaaaataagg taaacttttg
      121 agtgctttga aacattgata taatcgtatt gattaacgtt ttgaggcaac tgaccttgtc
      181 aggttgcagc cacatttgta agcgactaaa gtcgcaacaa ctgtgtcaat tgcaccaatt
      241 tagtaagaaa gtataaaaaa agaacacccc gaaaggtgct ctgtataagt atagtaattc
      301 tttcgaatta acgtttacta aattgtgatg ctttacgagc tttcttaaga cctggtttct
      361 tacgttcaac tttacgtgag tcacgtgtaa gaagtcctgc gcgtttcaat gaatcgcgga
      421 agtctgggtc tacttgaaga agggcacgag cgataccgtg acggatagct cctgattgac
      481 cagcgtatcc accacctaca acgttaacga aaacgtcgta tgaacctaca gttgaagtaa
      541 ctgcgaatgg ttggttgatg acaagacgaa ggtcagcgtg tgggatgtac tcttcaacat
      601 cttttttgtt aacagtgatt ttaccagttc ctggaacaag gcgaacgcgt gcaacagcgt
      661 ttttacgacg tccagtacct gcatattgtg cttgtgacat actttattgt tcctttcctt
      721 agataagttc tgaaatgtca agaacttctg gttgttgtgc agcgtgagtg tgctcagctc
      781 caacaaatac tttcaacttc ataccttgag cgcgtccaag tgtattgtgt ggaagcatac
      841 ctttaactga tttctcaatc aaacgtactg catttttaga acgaagttca cctgcagaga
      901 tttgtttcaa tccacctggg tggtttgagt gagtgtagta gattttatca gttgcttttt
      961 taccagtcaa tttaactttt tcagcattga taacaatcac gaagtcacct gtatcagtgt
     1021 gtggtgtaaa tgttggtttg ttttttccgc gaagtacgct agcaactact gcagaaagac
     1081 gtccaagtgg tacatcagtt gcgtcaacta cgtaccattt acgttcaact tggcctggtt
     1141 tagccataaa tgttgttttg ttcatgattt ctcctatata tagttcgatt tttgtttacg
     1201 gtgatgggtg ttccttccca tcgaaaagta tttggaaggt tccggggcct ttcaaatggg
     1261 gtaaacaata ccgcctacta tcataccaaa ttttacccct aaaagtcaat agatatgaaa
     1321 aatatttttc ggtaacttct caaagtaact gaaagttata taatcaagtt aatttgggaa
     1381 tttacctatt aacttcggta gatttttggg aatttttttc cttctttttc cctgtagtta
     1441 aaatcctttt caaaaaatca ccttttcttg atttttaatc ctatttgctg tatgataagg
     1501 gaaaagaaag gggacagaga tatggctttt accaataccc acatgcggtc tgctagtttt
     1561 ggcattgtta ccagcttgcc cgatgacatc attgactctt tttggtatat catcgatcat
     1621 ttcttaaaaa atgtctttga attggaagaa gaactcgagt ttcaattgct taataaccaa
     1681 ggaaagatta ccttccactt ttcaagtcaa cacctcccta cagccattga ttttgacttt
     1741 aaccatcctt tcgaccctcg ttatccccca agagtactgg ttttagacat ggacggtaga
     1801 gaaactatcc tcctcccaga agaaaatgac ctattttaat actcaatgaa aatcaaagag
     1861 caaactagga agctaaccgc aagctgtact tgagtacggt aaggcgacgc tgacgtggtt
     1921 tgaatttgat tttcgaagag tataaaaact ctagccttca gttgcaagtg actgaaaact
     1981 agagtttttc tattttttca aagcatcata caagttgcgg atcggttgtt ttaatatcgg
     2041 atggataaaa tgaggcgcaa tttcctgtaa ggactcaagg acaaaaaggc gttccgctat
     2101 gtaaggatga ggcaatatga ggtcgtctgt ataaaggatc tggtcctcca caaagagcaa
     2161 gtccaaatca atcaaacgag gtccccaatg cacttctctc acccgtccca gctctgactc
     2221 aatggctaac aaggtttcta acaagtcttg tgctggtagc caggtttcca cctcaaccac
     2281 ttgattggca aagctatcct gctccactcc accccaaggc tccgtcgcta agacactgga
     2341 ctctttgaga atatggatgc cacgagctcg cagtttgtca atggcttgct tcaagtttgc
     2401 ttgtttatct cccatattgc ttcctagggc gataaaggct cgttgcttgc ggcgatgaat
     2461 ggttaccgag caagtatcta gtgacaaatg caccggtgcc caaggttttt tcagttccaa
     2521 cttcatttct tggacaagag gataagactc aaaggtacgt tccaccagtt tataggctac
     2581 cgtttcaatc aaatcttcac tcgtttcctg aaaccaagtc gtccactgct gacacaattc
     2641 tccgtaatgg acagaggctg ttaaatccaa gtctgtagct gccttggtca tatcatagga
     2701 taggatggct gaaacgacaa atttctgccc caattctttc tcactaggaa aaagaccatg
     2761 ataggcaaac atttccaaat ccttaatctg cagttgatcc ataatgattc ctttctagaa
     2821 aaatccgctt gaagcggatt ctttttatag ccccattaaa cgataagctt ggtcacggag
     2881 atccttatct gtttcaaata gaccacgagc tactgtcgtc aaggttgcag tgcctggttt
     2941 tctaacacca cgcatactca tacacatatg ttccgcctca atgacaacaa aggctccttt
     3001 agcacctaga tagtccatca aggcatcggc cacttcgata ttcaaacgtt cttgaatttg
     3061 tggttttttc gaataaactt caaccgtacg ggctagctta gacaagcctg ccacacgacc
     3121 atctggaatg taggcaatgt gcgctctacc ataaaatggc aagaagtggt gttcacacat
     3181 ggtatggaaa aagatatcct tttctaccac catattatcg tcaataattt caaaggattt
     3241 tgacaaatgt tcctctgctg tttgaccaag acctgaaaaa atctcttgat acatacgggc
     3301 tacacgagca ggtgtttcct gcaagccctc gcgattagcg ttctctccta cagcctcgat
     3361 aatcattttt acagctgctt caatcttttg tgtatccatt ctcgttcttc ctctccatca
     3421 gataggccct cacttggctc aagaaataca aggaacctgt gacaatccta actgtttgtt
     3481 tctcttcttt tttatctgtc aaattctgct ctagaaaatc atgccaatct tggtagctga
     3541 gatttctagc cttagctgcc tctttcagca cgctttcatc cgtcgcccga ctatccgcaa
     3601 aatgtgttag agtaagctcg gtatctggca tggctcccag caagtccaac atatcctcca
     3661 aggctttggt tttgatacaa gtgaagagga tttccttatg atgatccgca aaacgttctt
     3721 gcaaggttac caacaaggcc ttgatagcat ggggattgtg ggctccatcc aaaatcatca
     3781 agggatctct tgacacgatt tccaaacgcc ctggccaact tgtttcttcc aaggcttgac
     3841 caagaaagtc attgcttgct agctctcgac catcttcttg acaaaaagta tcaagtaaag
     3901 ctatggccat cccagcattc tctatttggt acaaaccaag caggctagtc tggaagcaac
     3961 cttgtctgac agcacttgta tagtcaaaga cttcccctgt caccacactt tcttgatgac
     4021 gaacctgata atctgtcccg taggcaagtc tcggcgcatc tttctcttcc gcaatgcggt
     4081 caatcacagc taaggcttct ggagcaatac gccctgttac caagggcatc ccttgtttga
     4141 taatacctgc cttctgctct gctatggact ccaaggtgtc accaagtagg gccacatgat
     4201 ccaagccaat agttgtaatt cctgtcaaaa tgggctgaca gacattggta ctatccaaaa
     4261 gtccacccat gccaacttcc atgatggcca catctacttg ctctgaggca aagtagtcat
     4321 aggccagggc tgtgataatc tcaaactcgg ttgtgccctg taaattggcg accgcttctc
     4381 cctccagcaa agactgatag tctgccatga gagcttctag cctcgcttct gagatcgatt
     4441 ccccattgat gctaatctgg tctgtgtaat gaatgagata gggcgagcta aacacgccaa
     4501 ctctcaaccc tagcttttct agcatctttt tcaaaaaagc aatagtcgag cccttgccgt
     4561 tagtccctcc gatatggagg accttgagtt tgagatgggg attgccacgc aaagctaaca
     4621 gttccaccat tcgttccaag ccaaaatgcg gttgatccgt ccggtagtta gcaatccact
     4681 gattgttttc aatttctttc atcttattta tattgtttta aatctaaatt ttccgcttca
     4741 tcagccagac gaatggcaga ggcaatttca actgccatcc tgtgactagc tacgtcatgc
     4801 acgcgcacca cttctacacc ctgtctcgca gcgatactag ttacatgagc cgaagccgtg
     4861 tcccgatttc ggaaaccaag ctctgtctca ggattgactt caaaaccatt ctcctctagg
     4921 atattgatga caaatcgctt gcgcgacact ccgagaaaga ttggatagcc cttctgatgt
     4981 agtttatcca ggtcccgtaa aagaagcaga ttttctttct tggtcagacc aaagccaatt
     5041 cctggatcca acaggatatt ttctggtgca ataccagctt ctgccgctct cgctagtgct
     5101 cgttcaaaga aagcctccat caattcttcg attggcaatg tttcaaagtc cgctaactct
     5161 ttttctgtaa aggtttgacc aaaaccaaaa tgagggaaga taagcgaact aggatgctga
     5221 ggccgagcca taactgggtt aaacatgatg accacttgcg ctctcgcttc agctaccaca
     5281 tgagccattt tctcatcacc cataagacca gtgatatcat tgactagatc ggcaccagca
     5341 gccaaagcag cctctgctac ttgactcttc caagtatcaa tagagatgag gacatcactt
     5401 tccttgcgaa tcgctttgat cactggaaca acacgctgga tttcctcttc tatctcaaca
     5461 tagctactgc ccggccgagt cgattctccg ccgatatcga gcatactggc tccttctgct
     5521 atcaatttac gagcctgctg gagtgcctgc tcaagagcaa aaaattgacc accgtccgaa
     5581 aaggagtctg gggttacatt gataattccg caaatagctg tttttgcatg attggcttta
     5641 cttgacatat cggtcactcc ctcaaggctt ttcatcttat tatttttcta ttttaccata
     5701 aaaagaaaaa gatggacacg attgcattca tctttcccta gtagaaagag gtcggtttct
     5761 acataaaagc ttttaaacag aaattcctaa tgtccgactc ataatcacca caagagccaa
     5821 caaacagaaa gcaatcccat taacaatcat gtgaagcaag atcgacattt ccaaacgttg
     5881 ggtcttgtag gccgtccaag atagaactgt cgacatacct ccataaatca ataaagaagg
     5941 taaattactt ggttgatgca ataaagcaaa cacaatcgta ccgactacaa atcccaagtt
     6001 ctccttgcct cggaaaatct ttttaggaac aatcccacga cacaagattt cctcacaaat
     6061 cggagcaagc aaggctagca agaagaaact ggaaatcaac gaactatttt gaaccatatc
     6121 attaatctga gactggttag ctgttgtcgt ctcatttgac agttgcaaca agatagaacc
     6181 aagtatattt gacccgacaa taactaaata actcaaaccc aaacgtgcca aatctttagc
     6241 tctaaaaaaa gaaaaattaa aactagctaa cttggtttta cgagctccca taataaatag
     6301 agccagaacc acaattgaaa ggccagcaac catcagccct gactgtagca gggctacttc
     6361 ttttaaagtc aaaatagagg taaccactag gggaacctga tataaaagga cggcaagtaa
     6421 aaagaccaaa atccagcctg cacgattcaa caattctttc cacatatttt tctctttcat
     6481 cacctatctc ctttgtatta aaaaaggggg gaaatccccc tgggtcattc tattataagg
     6541 ccatgctaat gtagttaagg ataaacaagg catccaaaat ccaaatcatg gcatgaacat
     6601 ctttaacttg acctttaaca agcttagtca aagtgtaagt caagaaacca actgcaatcc
     6661 cttgagtgat agagtagctg aatcccataa agatagatgt gaagaaggca ggaactgctt
     6721 cagacatatc atcccaatgg atatttttca agctaccaag catcataatc ccaacgataa
     6781 tcaagattgg agctgtagcc gctgttggta cgatcgctag aagtgggcta aagaagcttg
     6841 aaatcgcaaa acagatagct acaaccaagg ctgtcaaacc agtacgtcca cctgcaccga
     6901 taccagcagc agactcaaca taagtcgtta cgtttgaagt acctgcaatg gcaccgactg
     6961 tcgttccaat caaatcagag taaagagcct tatccaattt ggctgattgg tgattttcac
     7021 catttgtcgc tacaatacca actttttcac ctgtaccgat caaggtacca attgtgtcaa
     7081 aaatatctgt caatgagaag gcaagaatgg ccatcagagt ttcaggcaag cgagctgtat
     7141 ctgaaaccaa agctcctaaa ccttctgaac caagagctgc accaaagatt gtcttcaaat
     7201 cttcaaaagc tgcaccaaca tggttattag caaaatcgat actagacaaa tcaaccaaac
     7261 caactgcaat agcaagaaca gttgttgtca agatagagag aataattccc cctttaatcc
     7321 ctttgatgac aaagaagata gtaatggcaa gtcctgcaag agccactaaa acagctggat
     7381 tattaaagct gaccaatcct ggaactgctg aagagtttgc tgcaatcgtt gcttgagctt
     7441 tgtcagcccc ttctcctaca acagtatagt tgcctggatc aatcgtgaat ttcaaaagtc
     7501 cagcattctt aatccctaca taggcaagga agacaccgat accagctgaa atagctgagc
     7561 gaagagcatt gggaatcgat tcaatgatca ttttacgaac ctttgtcaag gtaataatca
     7621 atgaaataat cccacagatg aagaccatag ctagggcttc ttgccaagaa taaccaagcc
     7681 cgaatacaac tgtaaaggta aagaaggcat tgagtcccat acctggcgct tgggcataag
     7741 gtaagttagc ataaaaagcc atcatcaagg tacccgctac tgcaccaata atcgtcgcta
     7801 ggaagacacc ctgagcaggc attcctgttt gtgaaagtat ttgtgggttt acaaagagaa
     7861 tatagctcat tgcaaagaaa gttgttaaac cagcgagaac ctctgtacga acgtctgtac
     7921 cgttctcttt tagtttaaat aatttgtcca tcatcaatct ccttttaatt tttacgaaca
     7981 ataatagaat tatatcattt atcattcact ttttcaatgt ctttgtttga tttgtttaag
     8041 aaattaatcc aatttcattt tttgtttgga atctgctttt ctgcctgctt tctgttataa
     8101 tagaagacat cttatctgaa aacacactag aaaggtccat atgacgaaaa aaattattgc
     8161 agttgacctg gatggaaccc tgctcaactc agacagtcaa atttctgact ttaccaaaaa
     8221 aaccatcaaa aaagttgctg aaaaaggcca tcaggttatt attacgacag gtcgccctta
     8281 ccgtatgtca aaagattttt accgtgaact gggcttagac actcctatga ttaacttcaa
     8341 cggatccctt actcatttac cagaccaagt ttgggatttt gaaaagtgtt tgactgtaga
     8401 caaaaaatat ctgctagata tggttcaacg ttcagaggac attcaagccg attttatcgc
     8461 tggagaatat cgtaaaaaat tctacattac aaatcccaat gaagagattg ccaatcccaa
     8521 actatttggt gtagaagctt tccagcctga agatcaattc cagcctgaat tggtgaccaa
     8581 ggaccctaac tgtatcctct tgcagaccag agccagtgac aaatatgcct tggcaaaaga
     8641 catgaacgcc ttctaccagc atcaactttc tatcaatacc tggggaggtc cgctcaatat
     8701 ccttgaatgt accccaaaag gtgtcaacaa ggcctttgct ttggactact tgctcaagat
     8761 aatgaatcgt gacaaaaaag atttgattgc ctttggagat gaacacaatg ataccgaaat
     8821 gctcgctttt gctgggaagg gttatgccat gaaaaatgcc aatccagagc tacttcctta
     8881 tgcagatgag caaatttccc ttaccaacga ccaagatggg gttgccaaaa ccctacaaga
     8941 cttattctta taacctatac tgatactcaa tgagaatcaa agagcaaact aggaagctag
     9001 ccgtaggttg ctcaaagcac tgctttgagg ttgtagatga agctgacgtg gtttgaagag
     9061 attttcgaag agtatgacca gcttgcaagc tggtctttgt gctcttttca cagtctcatc
     9121 aaccagttaa tcgattgttc tactttttgt ctccaaaacc ttggaaaagg ctttcttttg
     9181 tgatatactt cacatataaa tacaagagag ctcgtcacac tcgactctgt tgacaaaaaa
     9241 tcaatccagt cccgttgtcc ctgttctcgg tcaatatcaa ggaggatagc tatgaaagct
     9301 gttgttgtaa atccagaaag cactggtgtt gctattgaag aaaaagtact ccgtccactt
     9361 gaaactgggg aagcacttgt agaagttgaa tactgtggcg tttgccacac cgacctccac
     9421 gttgctcatg gtgactttgg tcaagtccca ggacgtgttc ttgggcacga aggtatcggt
     9481 atcgttaaag agattgctcc agatgtgaaa agccttaaag tcggtgaccg cgtcagcgtt
     9541 gcttggttct ttgaaggatg tggcacttgc gaatactgta caactggtcg cgaaaccctt
     9601 tgccgtacag tgaaaaatgc tggctactca gtagacggtg gtatggctga acaatgtatc
     9661 gtaactgctg actatgctgt caaagttcct gacggacttg atccagccca agcttcttct
     9721 atcacatgtg ctggagtaac aacctataaa gctatcaaag aagcaaaagt tgaaccaggc
     9781 caatgggttg ttctttacgg tgctggtgga cttggtaacc tcgctgttca atacgctaaa
     9841 aaagtattca atgctcatgt tatcgcagtc gatatcaaca atgacaaact tgcccttgca
     9901 aaagaagtag gcgctgacat tgtgattaac ggcctcgaag ttgaagatgt agctggactc
     9961 attaaagaaa aaactgatgg aggagctcat tcagctgtcg taactgctgt gtctaaagtt
    10021 gccttcaacc aggctgttga ctccattcgt gctggtggtc gcgtcgtcgc tgttggtctt
    10081 ccttctgaaa cgatggaact cagcatcgtt aaaacagtcc tcgatggaat ccaagtcatc
    10141 ggttctcttg tcggaactcg taaagactta gaagaagcct tccaatttgg tgcagaaggt
    10201 ctggtagtcc cagttgttca aaaacgtcca gtagaagatg ctgttgccat tttcgacgaa
    10261 atggaaaaag gccaaatcca aggacgtatg gtactcgact tcacccacta atctaataga
    10321 aacctatttt aaaagttgga agcttcttcc aactttttta ta
//
LOCUS       DAVLYR010000061        10196 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLYR010000061 DAVLYR010000000
VERSION     DAVLYR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.061"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            231..706
                     /locus_tag="VYO63_001170"
                     /pseudo
     CDS             231..706
                     /locus_tag="VYO63_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            744..1046
                     /locus_tag="VYO63_001171"
     CDS             744..1046
                     /locus_tag="VYO63_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1672095.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            1054..1855
                     /locus_tag="VYO63_001172"
                     /pseudo
     CDS             1054..1855
                     /locus_tag="VYO63_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1904..2368
                     /locus_tag="VYO63_001173"
     CDS             1904..2368
                     /locus_tag="VYO63_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1672096.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2525..2950
                     /gene="lacA"
                     /locus_tag="VYO63_001174"
     CDS             2525..2950
                     /gene="lacA"
                     /locus_tag="VYO63_001174"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1672097.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2981..3496
                     /gene="lacB"
                     /locus_tag="VYO63_001175"
     CDS             2981..3496
                     /gene="lacB"
                     /locus_tag="VYO63_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1672098.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3507..4436
                     /locus_tag="VYO63_001176"
     CDS             3507..4436
                     /locus_tag="VYO63_001176"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1672099.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4438..5418
                     /gene="lacD"
                     /locus_tag="VYO63_001177"
     CDS             4438..5418
                     /gene="lacD"
                     /locus_tag="VYO63_001177"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1672100.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5652..6488
                     /locus_tag="VYO63_001178"
     CDS             5652..6488
                     /locus_tag="VYO63_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1672101.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6524..6841
                     /locus_tag="VYO63_001179"
     CDS             6524..6841
                     /locus_tag="VYO63_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1672102.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6843..8537
                     /locus_tag="VYO63_001180"
     CDS             6843..8537
                     /locus_tag="VYO63_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1672103.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            8746..10152
                     /gene="lacG"
                     /locus_tag="VYO63_001181"
     CDS             8746..10152
                     /gene="lacG"
                     /locus_tag="VYO63_001181"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1672104.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTDYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         3303 a         1737 c         2101 g         3055 t
ORIGIN      
        1 gtgttttgag taacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta
       61 gaacagaaaa atgcttggag tatttgtttg tgtgtttatt tttatataac aaactataaa
      121 cacaacaaaa atataaaaaa agagacaaaa aagaacagaa agtaattaac aacgatttca
      181 tatagtgtta tacttatagc ataaagttaa tggaaagaag ggaaaacctt atggtattag
      241 attatttctt tgacaaaaac cttgtgtttt gcttagaagc ggataatcaa gaataactct
      301 ttgatcaggt agcaagcttg ttggaggaac gagaaattgt aactccaact tatcgtgaag
      361 ctttgatcac gcgtgaaaag tcatttccaa ctggtttaga tatggaattt ctaggaaagg
      421 acttgcaaat gtaatgattc ctcacacaga tattgttcat aatctagctg agaaagtggt
      481 ggttgttcga ttagagaaac cggtgacttt ccataatatg attgcgccag gtaaggaagt
      541 agaagtatcc ttgctcttct ttatcatcaa taattcaagt tcaagtcaaa cgaacattct
      601 tgctcagttg atggactttt tcacaggaaa tggacatctt gaagacctat caaaaatttc
      661 cgaaccagaa aaactttatg cttatataga tgaagcaatc gcttaatctt gtctattaaa
      721 aaataaaatc ggaggaagtc tatatgatta aaattcttgc tgcctgcggt gcaggtgtta
      781 actcaagtca ccaaattaaa agtgctctag aagaagaact ttcaaaccgt ggttacgatg
      841 ttcactgtga tgcagttatg gtgaaagatg taaacgaaga ccttatgaaa ggttacgata
      901 tctttacacc aatcgctgca acagaccttg gttttgaacc aggtatccca gttatcgaag
      961 ctgggccaat cttattccgt atcccagcaa tgagcgttcc agtatttgac aatattagac
     1021 ttcctgcgaa acaaaatatg gtatagtagt tctatgaatg atgaagcaag taaacaacta
     1081 actgatgcac gatttaagcg tcttgttggt gttcagcgta ccacttttga agagatgtta
     1141 gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta
     1201 agcctagaag accttcttat ggccactctt caatagtgcg agaatatcga acttatgaac
     1261 aaattgcggc tgattttggt attcacgaaa gcaactttat ccgtcggagc caatgggttg
     1321 aagtaactct tgttcaaagt ggtgttacgg tttcaagaac tcctctcagc tctgaggaca
     1381 cggtaatgat tgatgcgacg gaagtacaaa tcaatcgccc taaaaaagaa ttagcgaatg
     1441 attctggtaa aaagaaatgc cacgctatga aggctcaagc gattgtcaca agtcaaggga
     1501 gattgtttct ttggatatca ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag
     1561 tcgcagaaat atcggacaag ctggtaaaat cttggctgac agtggttatc aagggctcat
     1621 gaagatatat cctcaagtac aaactccacg taaatccagc aaactcaagc cgctaacagt
     1681 tgaagataaa acctataacc atgcgctatc taaggagaga agcaaggttg agaacatctt
     1741 tgccaaagta aaaacgttta aaatgatttc aacaacctat cgaaatcatc gtaaacgctt
     1801 cggattacga atgaatttga ttgctggcat tatcaatcat gaactaggat tctagttttg
     1861 caggaagtct aataaagaaa ctagcaaggt caggccttac tttattggtg gacatccagg
     1921 atttaattgt cctctacttg atgatgaagt ctatgaagat tattatctag agtttgagaa
     1981 agaagagact tgctctgttc cacgtccttt cccagaaaca ggtatgttgg atttccaaga
     2041 tagaagtcca tggctagagg gacaaaaaga aatagatctt agttatgatc ttttcagcaa
     2101 agatgcagtg actttggatg aattgcaatc tcgaacaatt gcccttcgtt ctcttaaaca
     2161 tgatagggga ttgaaagtgc attttgcaga atttccaaac ctcatcatct ggtcaacttt
     2221 gaacaaggga cctttcatta cctttgaacc atggtctggc ttgtcaacat tccttgaaga
     2281 aggagatcat ttagaagata agaagaatgt ttgtctctta gaagccaatc aggttgaaga
     2341 attagggttt gagatagaag ttttataaaa aagtaaaaaa taaataacaa aaacaaacac
     2401 aaactgttga cttcttcaaa caatagtagt atattatgtt tgtgaggact aattgactgt
     2461 tttttaaaga cagccaatac actgctggaa tccagcatag aaaaaataaa aaggagtata
     2521 caatatgtct attgttatcg gtgcagatgc tgcaggtttg agattgaaag aagttgtgaa
     2581 agacttctta gaaaaagaaa acttccacct tgtggatgtt acagctgaag gtcaagactt
     2641 tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa gaagaacaaa accttggtat
     2701 cgtgattgat gcttatggag ctggtccatt tatggttgca actaagatca aaggaatggt
     2761 tgctgcagaa gtttctgatg aacgttcagc ttatatgact cgtggccaca acaactcacg
     2821 tatgatcact atgggtgcac aacttgttgg tgatgaattg gctaaaaaca tcgctaaagg
     2881 atttgttaat ggtaaatacg acggtggtcg tcaccaaatc cgggttgaca tgttgaacaa
     2941 aatgggctaa ttagattata gtaagaaagg taagttaaaa atgagaattg caattggatg
     3001 tgaccacatc gtaacagatg aaaaaatggc ggtttcagaa tttttgaaat caaaaggata
     3061 tgaagtcatt gactttggta catatgacca tacacgtact cactacccaa tctttggtaa
     3121 aaaagtaggg gaagctgtaa ctagcggtca agctgatctt ggagtatgta tctgtggtac
     3181 tggtgttggt atcaacaacg ctgtaaataa agttccaggt gttcgttctg ccttggttcg
     3241 tgatatgaca acagcccttt atgctaaaga acaattgaac gctaacgtta ttggttttgg
     3301 tggtaaaatt actggtgaat tgcttatgtg tgatatcatc gaagctttca tccatgctga
     3361 atacaaacca actgaagaaa acaaaaaatt gattgcgaaa attgaacatg ttgaaagtca
     3421 caatgctcaa caaacagacg caaacttctt cactgaattc cttgaaaaat gggatcgcgg
     3481 agaataccac gactaagagg taacctatga ttttaacagt tacaatgaac ccatccattg
     3541 atatttccta tcccttggat gagttgaaga ttgatactgt caatcgtgtg gtggatgtaa
     3601 ccaaaacggc tggtggtaag ggactcaatg ttacccgagt actttcagaa tttggcgatt
     3661 ctgttcttgc tactggttta gtgggtggca aacttggtga gtttttggtt gaacatatcg
     3721 ataatcaagt aaagaaagat ttcttctcaa ttcagggaga aactcgtaac tgtatcgcta
     3781 ttctccacgg agacaaccaa acagaagttc ttgaaaaagg tcctgaagta ttggaacagg
     3841 aaggtcaaga ctttttggaa catttcaaaa aactcttgga gtcagttgaa gtagtggcca
     3901 tctcaggtag tctgccagct ggccttccag ttgattacta tgcgagcttg gtagaacttg
     3961 ctaatcaagc tggcaagcct gtagtcttgg actgctcagg tgcagcactt caggctgttc
     4021 ttgaatcacc ccataaacca acagtcatca aaccaaataa tgaagaattg tctcagcttc
     4081 ttggaagaga agtttctgag gatttggatg aattaaaaga agtacttcaa gaacctttgt
     4141 ttgcagggat tgaatggatt atcgtttcac ttggtgccaa cggtactttt gccaaacatg
     4201 gtgacacttt ctacaaggta gatattccta gaattcaggt ggtaaatcct gttggatctg
     4261 gagactctac tgtggcagga atttcttcag gacttcttca caaagaatcg gatgcagaat
     4321 tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca agaaaaaatg actggtcatg
     4381 tcaacatggc caactatcaa gttctatatg atcaattaat agtaaaagag gtataaaatg
     4441 gctttaacag aacaaaaacg tgtacgctta gaaaaacttt ctgatgaaaa tggtatcatc
     4501 tcagctcttg catttgacca acgtggtgct ttgaaacgcc tcatggctca acaccaaaca
     4561 gaagaaccaa ctgtggccca aatggaagaa cttaaagtct tggtagcaga tgaattgact
     4621 aaatacgctt catctatgct tcttgaccct gagtatggac ttccagcaac taaagctctt
     4681 gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag gttatgacac aacaagcaca
     4741 aaacgcttgc cagactgctt ggatgtctgg tctgcaaaac gtattaaaga agaaggtgca
     4801 gatgcagtta aattcttgct ttactatgat gtagatagct cagacgaact caatcaagaa
     4861 aaacaagcct acatcgaacg catcggttct gagtgtgtgg ctgaagatat cccattcttc
     4921 cttgaaattc ttgcttacga tgaaaaaatt gcggatgcag gttctgtaga atacgcgaaa
     4981 gtaaaaccac acaaagttat cggcgctatg aaagtctttt cagacccacg ctttaacatt
     5041 gatgtcttga aagttgaagt tcctgttaac attaaatatg ttgaaggctt cgctgaaggt
     5101 gaagtagttt atacacgtga agaagcagca gccttcttca aagcgcaaga tgaagcaacg
     5161 aacttgccat acatctactt gagtgctggt gtatcagcta aactcttcca agatactctt
     5221 gtatttgctc atgaatcagg tgcaaacttt aacggagttc tttgtggccg tgcaacatgg
     5281 gctggatcag ttgaagcata catcaaagat ggtgaaacag cagctcgcga atggcttcgc
     5341 acaactggat ttgaaaacat tgatgagctc aacaaagttc ttcaaacaac agcaacttca
     5401 tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa catgaatcta aaaaaattta
     5461 aaaaaagttg tatgtaaagg tttacaaaat aacttacttg tgctatactt aaatcacaag
     5521 ttaatacaag gtgagtgtta ctaagtaata ttaggcatga tcacaggtga attagaaatc
     5581 agctgatttt ctagttcatt tgtggtcatt ttttgtactc atataccttt aagatataaa
     5641 aggaggttga catgtatcga attctaaatc caatgaatca caatgtctcg cttgtcagaa
     5701 atgataaggg agaagaggtg attgtaattg gtaaggggat tgcattcgga aagaagaagg
     5761 gggatttgat tgctgaaaat catgttgaga aaatctttcg gatgaagacc gaagagtcca
     5821 gagaaaactt tatggctctt ctcaaagatg ttccgcttga ttttatcaca gtgacctatg
     5881 aaatcattga taagctatca aagaaatatc attatccgat tcaagagtat ctctatgtaa
     5941 ccttgacaga tcatatttac tgttcttatc aagctctaac tcaaggaagg tacaaggata
     6001 gtaatctgcc agatatttct gctaagtatc ctgtcgcttt tcaaatcgca aatgaagctt
     6061 ttgaaattta ctgtcagaag ctagcagatc attttcctga ggacgaaatt attcggattg
     6121 cttatcattt cattaatgct gaaggtgaaa atgaagtgga acttgtggag tcgattgata
     6181 agaggaaaga aattctcagg aatgttgaag aagttttaac ggactatgca attcaacgaa
     6241 ctaaaaagaa taaccatttc tatgatcgct ttatgatcca tttgaattat ttcttggatt
     6301 atttagacag atctagagat gataaccaat cacttctgga tatggaagat catattaaac
     6361 aatcctatcc aaaagccttc gagattggtt ccaagatcta tgatgtgatt acgcaacata
     6421 cgggtcttga tttgtataaa agtgaacgag tttatctagt tctacatatc caacgtttat
     6481 tgtcataaaa atttatttaa aactatataa ggagaattct atcatgaata gagaagaagt
     6541 aacattgtta ggttttgaaa tcgtagccta tgctggcgat gctcgttcaa aactattgga
     6601 agccttgaag gctgctgaag ctggtgattt tgaaaaagcg gacgctctgg tagaggaagc
     6661 tggtagctgt attgcagagg ctcaccacgc gcaaacaagt ctattgacta aggaagcttc
     6721 tggtgaggac ttagcgtata gtgtgaccat gatgcatggt caagatcact tgatgacaac
     6781 tatcttgtta aaagacctaa tgcaccattt aattgaactt tacaagagag gagttaaata
     6841 aaatggataa gttaataatt tttattgaga aaggaaagcc tttctttgag aaattatcga
     6901 gaaatatata cttaagagcg ataaaagatg gttttatttc cagtatgcca gcagtattat
     6961 tctcaagtat ttttatacta atagctgctg tgcccaatat ttttgggttt aaatggtctg
     7021 atgaacagtt agcttttatt ttgaaacctt acaactactc aatggggatt ttagctctct
     7081 tagtagctgg gacaactgca aaatcgttaa cagattcagt taatacacgt agtatggaga
     7141 aaaccaacca aatcaactac atgtcaactt ttttagcagc tgttgttgga ttgttaatac
     7201 tggcagctga tccaattgaa ggtggatttg ctaatggtct tttaggtact agaggattac
     7261 ttactgcatt tttggcagct tttattacag taaatatcta taaagtatgt attaaaaata
     7321 atgtaaccat tagacttcct gaagaggttc caccaaatat tgcacaagtt tttaaagatg
     7381 ttattccgtt tgccttatct gttctttcaa tatatggatt ggatttaatt gttagaaata
     7441 tttttggtac aaatgtagca gaatctgttg gtaaaatatt agcccctcta ttttcagcta
     7501 ctgatggtta tatcggttta gctattgtat ttggtgctta tgctttcttt tggtttgttg
     7561 gaattcatgg gccatctgtt gtagaacctt taattgtagc tatcagttat gcaaatatag
     7621 aagctaatgt tcagcttgtg caagctggta tgcacgcaga taaaatttta aatccagtta
     7681 ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac actagtagtt ccatttatgt
     7741 ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg acgtgcgtct gtagttccta
     7801 ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc aattgtttta aatcctatct
     7861 tctttattcc gtttgttact gctcctatta tcaatgtttg gattatgaaa ttttttgtag
     7921 atgttctaca aatgaatagt ttcagtatta ttttaccttg gacaacacct gctccaattg
     7981 gtattgttat gggaacggcg ctggctccat tgtcattcgt cttggctata actttaatca
     8041 ttatcgacac tttaatctat tatccatttg ttaaagttta tgaccatcaa attttagaag
     8101 aagaacggaa aggaaactct tcatctgaat tgaaagaaaa agttgctgca aacttcaaca
     8161 ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtaga agcagcacaa aatacaatca
     8221 ctaaagaaac aaatgtcctc gttctctgtg caggtggagg aacaagtggt ctccttgcaa
     8281 atgctttgaa taaggcagca gcagaataca atgtccctgt gaaagcagca gcaggcggct
     8341 atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt tatccttgcc cctcaagttg
     8401 cttcaaactt tgaagatatg aaagcagaaa cagataagct cggtattaaa ctagcgaaaa
     8461 cagaaggcgc tcaatacatc aaattaactc gtgatggaaa aggtgctctt gcattcgtac
     8521 aagcgcaatt cgattaaggc tagagactct gaaatagtct cctatcgtta cggaaatcgc
     8581 tatggcgaat ttcctattat taattcgtcg gtaaaaagat atcgttttta cctcctcatg
     8641 tcacaattcg gtgacttggt acaagaagtg agatggagaa ggatggctca ttgactcctc
     8701 tcctctcact tttactttat ttaaatcaag aaataggtga aaaaaatgac aaaaacactt
     8761 ccaaaagact ttatttttgg tggcgcaaca gctgcctatc aagcagaagg tgctacacat
     8821 actgatggta aaggaccagt agcgtgggat aaatatcttg aagataacta ctggtacact
     8881 gcagaaccag ccagtgattt ttacaatcga tatccagttg acctcaagct agcagaagag
     8941 tatggtgtca atggtattcg aatttctatt gcttggtcac gtattttccc gactgattac
     9001 ggccaagtaa atgctaaagg tgttgagttt tatcataatt tatttgcaga gtgtcacaaa
     9061 cgtcatgttg agccttttgt aactcttcat cactttgaca cgccagaagc tctccactca
     9121 aatggagact tcttaaaccg tgaaaatatc gaacattttg tagactacgc tgccttctgt
     9181 tttgaagaat ttccagaagt aaactattgg acaaccttta atgaaattgg accaatcggt
     9241 gatggtcaat atttggttgg gaaattccct ccaggtatcc agtacgacct tgccaaagtc
     9301 tttcaatcac accacaatat gatggtgtct catgcacgcg cggtaaaatt gtacaaagag
     9361 aaaggctata aaggggaaat tggtgttgtt cacgccctgc caactaaata tcctctagat
     9421 cctgaaaatc cagcagatgt tcgtgcagct gagttggaag atatcatcca caataaattc
     9481 atcttagacg caacttatct aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat
     9541 atcttatcag tcaatggtgg tagcttggat cttcgtgaag aagattttac agcattagaa
     9601 gctgcaaaag acttgaatga tttcctagga atcaactact atatgagtga ctggatggaa
     9661 gcctttgatg gagaaactga aattatccat aatggtaaag gtgaaaaagg aagctctaag
     9721 tatcaaatca aaggtgttgg tcgtcgtgta gctcctgact atgtaccacg cacggattgg
     9781 gattggatta tctaccctca aggtttgtat gaccaaatca tgcgtgtgaa gaaagattat
     9841 cctaactaca agaagattta catcactgaa aatggtctcg gctataaaga tgagttcgtt
     9901 gataacactg tttacgatga tggtcgtatt gattacgtga aacaacactt ggagatcttg
     9961 tctgatgcga ttgcagatgg agctaatgta aaaggttact tcatttggtc attaatggat
    10021 gtcttctcat ggtcaaacgg ttatgagaaa cgttatggtc tcttctacgt agattttgaa
    10081 actcaagaac gttatcctaa gaaatcagct cactggtaca agaaagtagc ggaaactcag
    10141 attatagact agtagaatta gtcattagat atagaatttt agtgagtcaa aaagat
//
LOCUS       DAVLYR010000062        10061 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLYR010000062 DAVLYR010000000
VERSION     DAVLYR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.062"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(658..774)
                     /locus_tag="VYO63_001182"
     CDS             complement(658..774)
                     /locus_tag="VYO63_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1672105.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(989..1342)
                     /locus_tag="VYO63_001183"
     CDS             complement(989..1342)
                     /locus_tag="VYO63_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1672106.1"
                     /translation="MTVRHPGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAQNVTIL
                     EGSNIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            complement(1460..2428)
                     /locus_tag="VYO63_001184"
     CDS             complement(1460..2428)
                     /locus_tag="VYO63_001184"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1672107.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2573..3133)
                     /locus_tag="VYO63_001185"
     CDS             complement(2573..3133)
                     /locus_tag="VYO63_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV1672108.1"
                     /translation="MSNFLGSVHLLGVLFPDSTFLNAFESAIVAPLVEESLKLLPLVF
                     VLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIHTDLPEGFDFTISRILERIISGIAS
                     HWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAI
                     PVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(3139..4490)
                     /locus_tag="VYO63_001186"
     CDS             complement(join(3139..4049,4049..4490))
                     /locus_tag="VYO63_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1672109.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNSMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
     gene            complement(4487..4831)
                     /locus_tag="VYO63_001187"
     CDS             complement(4487..4831)
                     /locus_tag="VYO63_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672110.1"
                     /translation="MYEEKQYFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDFMNCTPKV
                     RQKKSNFWGVLL"
     gene            complement(4852..5349)
                     /locus_tag="VYO63_001188"
     CDS             complement(4852..5349)
                     /locus_tag="VYO63_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1672111.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(5422..6783)
                     /gene="radA"
                     /locus_tag="VYO63_001189"
     CDS             complement(5422..6783)
                     /gene="radA"
                     /locus_tag="VYO63_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1672112.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYISGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(6797..7312)
                     /locus_tag="VYO63_001190"
     CDS             complement(6797..7312)
                     /locus_tag="VYO63_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1672113.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     TPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(7314..7757)
                     /locus_tag="VYO63_001191"
     CDS             complement(7314..7757)
                     /locus_tag="VYO63_001191"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1672114.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(7817..7910)
                     /gene="ffs"
                     /locus_tag="VYO63_001192"
     ncRNA           complement(7817..7910)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYO63_001192"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(7943..8410)
                     /locus_tag="VYO63_001193"
     CDS             complement(7943..8410)
                     /locus_tag="VYO63_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1672115.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(8611..9897)
                     /locus_tag="VYO63_001194"
     CDS             complement(8611..9897)
                     /locus_tag="VYO63_001194"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1672116.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         3045 a         2403 c         1683 g         2930 t
ORIGIN      
        1 actttcctga tagagttgtt tcacatctta tttcaactca ctatagaagg aggaaaagga
       61 gggctgggtg gctaacttca ttatagaact ttcaaaaaga tgaaaaaata gcgtcaaaga
      121 ggcttttttc tctttaaaac aggaaatttc aaagacaatc ttgcgactat ccaaactgta
      181 cgaatagcga gacataactt acttcgctaa tactatagta gattgaaact agaatagtac
      241 acctcgattt ctaaaacatt gttagaaatc gatttgactg tcctgaacga tttgtcctgt
      301 tattatttca ttttactata tcttataaaa agatagaaat gtcaagcaaa aatgacaagt
      361 attttgataa taaacttgcc atcccatcaa ctttctacta tataaagtat gaactcatag
      421 caaggactca aactcagcca cggtcatgcc taattgctca ctcgcaacct caggtgttag
      481 aagaccttga cgtactagat ttactaaacc tacttttaat ccctcttcaa ggccttcttc
      541 tcgtccttgt tcacaaccct ctgcacgacc acgttcaata ccacgctcta gtccttgttc
      601 aataccttcg catagtcctg tgctaacaag acttgttctt ctcgcatatg tagttgacta
      661 aacattttcc tgtcctcctc ggaccagctc ttgtagtcca gcagttgatt tgcttgggta
      721 atggctcgtt gcggttgctg ggtaaagggc ttgttgccga aaaactccaa ccatggcttg
      781 cgaaccttgt ctttgctggt ttctctgtat tttttaattc caagaatgcc atcttaacca
      841 gatggttttc ctgtccattg ttggtaatcg ccaatacctc acctgttgtg tcttcgcgca
      901 tactaacgct atgaaaagcc aggccatctg agaaataatt gctatccacg atagcaatga
      961 cgtaaacagg agcgatgtgt ttgtagatct agtgagtatc accttctcgc tgacgaattt
     1021 tttcaagatt ttgattaacc tgactgcaca ggtaagccca caagtgattg atgaaaaaat
     1081 tctgatgatg gacttgaatc tcaataatta cttgagtacc gttatccaac tccgccaaga
     1141 cgtctatact ggtataaaaa tcctgcaccg agtaaggcat ggagagtaat acgtgaatat
     1201 tgctaccctc caaaatggtc acattttggg ctggcaaatc cagcatatcg cgaataaatt
     1261 gacaagtaat ttctggattg ctaaagattt tcttagctac caagtcattg gttgggctaa
     1321 tgcccggatg acgtacagtc atatttcttc tctttttatt atatcacagt tttaaatcta
     1381 gctttactag attcaccgct actatctatt tattcggaaa aaagacgaaa aaacctgaga
     1441 atcatctcag gcttggtcat taaatttttt tctcaatatc gaaaagtgga gaaagtggtc
     1501 gtttttcatg aatacgtacg atagcatccc ctaggagatg agcgattgaa atctgctcaa
     1561 tcttatcaat caaacgctct tctggcagat agatggtatc caaaacaacc aatttcttaa
     1621 tagctgattt ttggatattg tccgtagcag gaccagaaag aactgggtgc gtacagcttg
     1681 catagacttc aacagcacca gcttccgcaa gagcatctgc cgcatgacaa atcgttccag
     1741 cggtatcaat catatcatca atcaagatac aagtcttgcc ttcaacctta ccgatgatgt
     1801 tcataacttc actagtattc atcttatcaa cgctacgacg tttatcaata atagcgatag
     1861 aagttttcaa aaattctgcc aacttacgag cacgagtcac ccctccatgg tccgggctga
     1921 caaccacata gtcagaacca accataccac gacgctcaaa ataatctgca atcagaggag
     1981 cacccatcaa atgatccaca ggaatatcaa agaatccttg aatttgcgca gcatgcaagt
     2041 cgatggtcaa taaacgatcc actccagcta cttcaagcat atttgcgaca agttttgaag
     2101 tgattggctc acgcgctctc gcctttctat cctgacgtgc atacccatag taaggcatga
     2161 caacattgac agattctgca ctcgcacgct tcaaagcatc taccataatc aaaatttcaa
     2221 gcagattgtc atttacaggc gaactagttg attgtaggat aaagacgtgt ttcccacgga
     2281 ttgattcttc aatgttgacc tgaatctctc catctgaaaa ttggcgaaca cttgatttcc
     2341 ccaactctat cccaatctcc tgcgccacac gttctgccaa ttctttatta gaagaaaggg
     2401 caaatagctt taaatcagaa aaagacatga tttcctccgg tatatatgta taacttgtgc
     2461 ttttcacaag attttccatc taccattgta gcgctttttg cactattttt caatcaaaaa
     2521 taaaagaagg gcaccatatt tgtacccttg catcattctt ttgaaaaata ttctaggtca
     2581 tcaactcatt gtgtttctca acaaagcaat aagcatgata aaaaccatag agagcaatag
     2641 ccgtaaccac tggaatcgct aaaggcaact ctgtttccaa ctccacaaaa ggagagttaa
     2701 acaagaagtg agttcccaag gctaaaccta gaaaaataag gccctgtttc ttgccaacct
     2761 tctgtccttt ataggctctg taaagcaagt aaacacctac tacagctaga cctgaaaaag
     2821 tccagtgaga ggcaattcct gagatgatac gctctaaaat tcgcgaaata gtaaagtcaa
     2881 agccctctgg caaatccgta tgaatataac caatatcctc aatcatttgg aatcccaaac
     2941 cggaagcaat tccaagtaaa aacaaagatt ttaattttcg cacaggaatc aaagccaaaa
     3001 caaaaacaag tggcaataat ttcaaggatt cttctaccaa aggagccaca atagcacttt
     3061 caaaggcatt taaaaatgta ctatctggga aaagaacccc cagtaaatgt actgacccca
     3121 aaaagttaga caaataattt atccaaagga tttagttctg tactgcacag aactaagtcc
     3181 ttttagtttt accttaattc gtttgttgtt gtagtaatca atgtagtcca caatagcttg
     3241 ttcaaggttt tctaaagatc taaagttctt ctcatagcca taaaacattt cggattttaa
     3301 aatgccaaag aaagattcca tcatgctgtt gtctgggctg ttgcccttgc gtgacatgga
     3361 tgcttgaatt cccttactct ctaggaaccg atgataagaa tcgtgttggt attgccagcc
     3421 ttggtcacta tggagaatcg tattctcata gtatttctct gtaaaggcct gttctaacat
     3481 actcttcact tgttctaagt ttggcgaagt agaaagatga taagcaatga tttcactgtt
     3541 aaaaccatct aaaactggtg ataaatagag tttctgtgtg ctatttggaa tggcaaactc
     3601 tgtcacatct gtatagcact tttccattgg cctcgatgct tcgaactggc gttgaatgag
     3661 attctctgct ttcttgccaa tctctccttg gtaggaagaa tacttccgtt tacggcgaat
     3721 tcgagccgtt aaaccaagaa ttctcatcaa ccgttgaact ttcttatgat tgaccacaaa
     3781 accacgattc cttagttcta aatggattcg acgatagcca tagttgcctt tatgttcatt
     3841 atcaatgccc tgaatttcgg ttttaatctc tttatcttta tcaaccccat ccagttgttt
     3901 caactgataa tagtaagtcg agcgagctaa acgtgctgtt tcaagaagaa aatctagtcg
     3961 aaatcctcct gaaaccattt ctctaactgt ctctgccttt ctcgctctag ggcttcgtcc
     4021 ctttcctcta actcttttaa cttttttagg taggccacct cagtccgtaa gcgttcattc
     4081 tcctcctgga gtcgttctag ttctgtcatt tcttcccaag tcttcttccg tttacgtccc
     4141 attttagctg gtctccctct tgttttctca acaatagtat acccgttttt cttgtattgt
     4201 gccggccaaa atgaaagcat tccttggttt gggagggcat aatcaagact aatacttctt
     4261 tgtgaacaac cttccagtaa ggctttatcc atcatttctt gtttcaattt agaagaatag
     4321 tgacgattct tccctttttt aacgatttct atgccataac ggtcaattaa tttcaccatg
     4381 tactttagac cagaaacatc cacaccaaat ctttttgaaa gctgtttgaa gctttgtcct
     4441 tgctttctta gttcatagat ctgaacttta tcttcataac tcaatttcat aataaaacac
     4501 cccaaaagtt agattttttc tgtctaactt ttggggtgca gttcataaaa tcatggatat
     4561 aagtattagc aaaactagac aaccagcctg aaaggaacat ccctcccaat aaagacagaa
     4621 tcaaaacctt ctttggcaat tcccattttt tcccaatacg gaagagaaaa taaagagccg
     4681 gaatcatgta aaagagagct agaaagatag aaactcccat tagtccatat tccgcacctg
     4741 acctcgaacc gtccgtatag tagatggttt catactgtaa accaatacat agcaataaaa
     4801 taaaaataaa atactgcttt tcttcataca ctttctttct aaatgaagta tttataattc
     4861 tacgactgtc atacttcctg tatcaacatt gtaaatagca ccagagataa tgacatcgtc
     4921 tggtattagg ggagactcga taagcagttg catatcctcg cgtacactct cttctatatc
     4981 ttggaagggc aagaagtcct ggtctgacac atctacacct aattcctctt ttaaatactc
     5041 ctgaaaaggt tcattttcaa aggtctgagc accacagtct gtatggtgca ataccacaat
     5101 ctctcttgtc cccatttgtt gctgggaaat aactagcgaa cgaatcatgt cttcagtcac
     5161 tcgaccacct gcattccgca agatatgagc atccccaagt gccaaaccta gagcttgcgc
     5221 aacgtgcaaa cgtgagtcca tacaggtcac aatagctact cttgttttgg gtttaagtgg
     5281 cagatttaac tgcccatgta gggcaacata agcctgatta gcttgcataa actgttcaaa
     5341 atacgacacg attccctcct tgaaaatttg atagtcaaat atttctccta tcttatcatt
     5401 tttaagagaa tttgtcacgg attatgcaaa gacctttttc aaaacttcct gaatcgttgt
     5461 cacgccaatg acctgaattt ccttaggcag agtgattcct gtcaaggaat tcttaggtac
     5521 ataaatctta gtaaagccca gtttagcagc ttcgttgatg cgttgctcaa tacgattcac
     5581 gcgccgaatc tctcctgtca agcccagttc tccgacaaaa cattcctgag gattagttgg
     5641 cttgtctttg tagctcgaag caatagcaac tgcaacagcc aagtcaatgg caggttcatc
     5701 caatttaaca ccaccagcag atttgagata ggcatcctga ttttgcaaga gaagccctgc
     5761 ccgtttttcc aaaacagcca taatcaagct agcacggtta aaatcaagtc ctgtcgtagt
     5821 acgcttggca tttccaaaca tggtcggtgt taccaaagcc tgaacctccg ccaaaatcgg
     5881 acgcgtccct tccatggtta caacgatgga ggaaccagtt gccccatcca aacgctcttc
     5941 taggaaaact tgactcggat tgagtacctc aaccaagccg cccgactgca tctcaaaaat
     6001 cccaatctca ttagtggaac caaaacgatt tttgaccgct ctcaaaatac gaaaggtgtg
     6061 gtgacgctcc ccttcaaagt aaagcaccgt atccaccata tgctccaaca tacgaggccc
     6121 agccaaggtt ccttctttgg tcacatgacc tacgataaag atggcaatgt tattggtctt
     6181 ggccaactgc atgagttcag cggtcacttc acgtacctga gaaacagacc cctgcacccc
     6241 tgaaatctca ggagacatga tggtctggat ggaatcaata ataagaaagt ctggctggat
     6301 acgctccact tctgcacgaa cactctgcat attggtctct gcatagagat aaaactcact
     6361 atcaatatca cctaagcgct ctgcacgtag tttaatctgc tgggcagact cctccccact
     6421 gatatagaga actgtcccca cttgggacaa ctgggttgag acttgtagga gaagagttga
     6481 tttcccaatc ccaggatccc caccgatgag gacgagactt cctggtacca ctccgcctcc
     6541 aagcacacgg ttgaattcct ccatctccgt cttggttcga ttgacattga tggaagtcac
     6601 ctcagctagt ttcatgggct tggttttctc acctgtcaag gacacacgcg cattcttgac
     6661 ctcggcaacc tcaacctctt ccacaaaaga agaccaagac ccacagttgg ggcaacgtcc
     6721 cagatattta ggggaattat acccacaatt ttgacataca aatgtcgctt ttttctttgc
     6781 gatgacaaac ctctttctat atctctaact cacactcaat cacttggcaa aaatcaatct
     6841 tctcatttgg cacaaactgg cgcatgagca ttcgatgagc aacaactacc acagtctgat
     6901 gttctcgata cttagacata cattctagaa accgagactt catttccgta gctgtctcat
     6961 attgaatagg actattagga agcaactccc ccttgttttc taaaaacagt cttctagctg
     7021 tttcaaagtt ttctattcct gttttataga cctgccattc atgtaataaa ggctctactc
     7081 ttaaaggaag acccgtagca cagaccacat acgaagccgt ttctaaagct cttgtgactg
     7141 cagaagatac gattatttca gctgacgaga gtaaaggagt tttgctcaat ttctggactt
     7201 gctgccgtcc catctcagac aagggtgcca aatctatccc aaatcctata taagaacgct
     7261 cctctaactc acggtaatct ggctccccat gacgtacaaa gataatcttc attctagtgc
     7321 cctgtcgatc caaatccacc agttcgaacg ccatcagctg catctccatc tgcaattaag
     7381 aaagtagcaa aaacagcctg gacaatacgt tccccaactt caagaacaac ctcttggtct
     7441 gtgatattct tcatctgcgc aaaaatatgc ccttcatttc caggatttcc ataataatcc
     7501 ccatcaatga ccccaactga gttaattaaa accaagccct tcttacgagg atttgaagaa
     7561 cgatcataga ggtagagaac ctcagtcggc tgcatataag ccttaacccc tgtcggaacc
     7621 aagacaatct ctcctggcgc aacaactgta cgcacagcaa cctttaagtc gtaaccagcc
     7681 gcatgcgctg tctcacgctt gggcaataaa ttttcatctg taaaactcga aaccaattca
     7741 aaaccacgaa ttttcataat tttctctttt ctattatcat ttattctaga ttattctatc
     7801 ttatttattc ggaaaaagca cgaaaaaaga gcacacaatt caaatcgctt agggctgctg
     7861 gattcctccc ctgacccgct tcacgcagaa ctgttgctcc actatttatt atatcacatt
     7921 cctattcatt ttaaaagcaa aattattttt tccgtctatt tctaaaaaaa tcctgcatga
     7981 tagctgcgca ttcatcttcc aaaattccta tttcaacctc cacacgatga ttgagacgct
     8041 catctgtcaa gatattgtac aaactcccag cagcgccaaa tttctggttt ttagccccat
     8101 agaccacatt tggaatgcgg gcaagcccaa tcgctccact acacatgaca caaggttcaa
     8161 tggtcacaaa aagtgtgcaa tccagcaagc gccagctctc ctcactcaag ttcgcatcct
     8221 ctatagccat aatttccgca tgcataaccg ctcgctgtaa ttcctcacgc gcattatgcc
     8281 cacgaccaat gatttcccca tctttgacaa tcacacaacc aattggaatt tcatcgtgtt
     8341 caagagcaat ctcagcctct ctcaaagcct ccctcataaa gacttctttt tcttcaagcg
     8401 tataatacat ctcttttctc ttttcctact tatcgatttt attattatat catgaatccc
     8461 aacacaaaag aagccaccga atgcggtgac tctataggga gattattatg aaaaagaaaa
     8521 gtttaggata tttgttacaa caagttagga ggtcttcttg taaccgtcta tagtataccc
     8581 gacctatctt aaacaaatct taaaaatctc ttaggaccaa acactttcta aaatatttgt
     8641 ttgttcacga ccaggaccta ctgagaaagt agaaatacga acgccaacca attcactcac
     8701 acgacgaaca tagttacgcg cattctcagg aagatcttcc aaattgcgaa ctccggtaat
     8761 atcttctgac caacctggca actcttcata gataggcttg caacgtttca attgctcaag
     8821 actagctgga tagtagtcaa tacgttgacc gtcaagatca taggccacac agattttcac
     8881 agtatccaaa ccgctcaaaa catcaataga gttcaaagaa aggttagtaa taccagaaac
     8941 acgacggcta tgacgcatca caactgagtc aaaccaacct acacgacgtg gacgaccagt
     9001 tgttgtacca tattcatgac ccacttcacg gatacgttct cccacttcat caaacaactc
     9061 agttgggaaa ggaccatctc ctacacgact cgtataagct ttacatacac ctacaacctt
     9121 gtcaatcttg cttggaccga caccagaacc aattgtcaca ccaccagcta cagggtttga
     9181 tgacgtaaca aatggataag taccttggtc gatatctagc ataacacctt gtgcaccttc
     9241 aaaaagcaca cgtttgccat tatcaagcgc atcattcaag ataacagatg tatctatcac
     9301 gtatttcttg atttgttgac catattcgta atattcttca aaaatatcat cgaaaacaat
     9361 cgctttactg tcatacaatt tttcaaaaag acgattcttt tcagcaaggt tacgttctaa
     9421 acgctcacgg aaaatatctt tatctaaaag atctgcaata cgaattccaa cacgagcagc
     9481 cttgtccata taagctggac caattccctt aattgtcgta ccaatcttat tgtcgccctt
     9541 agcttcttct tgcaagcgat ccaactcgat atgataaggc aaaataacat gcgcacgatc
     9601 agaaatacgc aagttatcag ttgttacacc ttcctcatga agatagctca actcttttac
     9661 aagagattta ggatttacaa ccataccatt cccaatgaca gatatttttt cagggaagaa
     9721 aatcccagat ggaatcaagt gcaacttaaa tttcttaccg tcaatcacaa tcgtgtgacc
     9781 agcattatca ccaccttggt aacgtgcaat tacttctgca ttcgctgaaa ggaagtctgt
     9841 aatcttccct ttaccttcat caccccattg ggtacctaca acaacaactg aagtcataat
     9901 tttgtctgag ccctcaggct cttcctttct cacatacatg gcaggactct cacctgcaat
     9961 tatatcttac aatttattat aataaaaaat cgccttttta tcaagaagaa acaatagaaa
    10021 gatttgctat ttccaactat taaaaaatga tttagaaaaa t
//
LOCUS       DAVLYR010000063         9947 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLYR010000063 DAVLYR010000000
VERSION     DAVLYR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.063"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            300..683
                     /locus_tag="VYO63_001195"
     CDS             300..683
                     /locus_tag="VYO63_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1672117.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            735..1424
                     /locus_tag="VYO63_001196"
     CDS             735..1424
                     /locus_tag="VYO63_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1672118.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1466..1699
                     /locus_tag="VYO63_001197"
     CDS             1466..1699
                     /locus_tag="VYO63_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672119.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1850..2461
                     /locus_tag="VYO63_001198"
     CDS             1850..2461
                     /locus_tag="VYO63_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1672120.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2622..3416
                     /locus_tag="VYO63_001199"
     CDS             2622..3416
                     /locus_tag="VYO63_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1672121.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3413..4048
                     /gene="trmB"
                     /locus_tag="VYO63_001200"
     CDS             3413..4048
                     /gene="trmB"
                     /locus_tag="VYO63_001200"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1672122.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQDPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4174..4653
                     /gene="rimP"
                     /locus_tag="VYO63_001201"
     CDS             4174..4653
                     /gene="rimP"
                     /locus_tag="VYO63_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1672123.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMGKTRKKTVQIPYSLVSKARLAVKL"
     gene            4697..5833
                     /gene="nusA"
                     /locus_tag="VYO63_001202"
     CDS             4697..5833
                     /gene="nusA"
                     /locus_tag="VYO63_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1672124.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5855..6148
                     /locus_tag="VYO63_001203"
     CDS             5855..6148
                     /locus_tag="VYO63_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1672125.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6141..6440
                     /locus_tag="VYO63_001204"
     CDS             6141..6440
                     /locus_tag="VYO63_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1672126.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6457..9249
                     /gene="infB"
                     /locus_tag="VYO63_001205"
     CDS             6457..9249
                     /gene="infB"
                     /locus_tag="VYO63_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1672127.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9500..9850
                     /gene="rbfA"
                     /locus_tag="VYO63_001206"
     CDS             9500..9850
                     /gene="rbfA"
                     /locus_tag="VYO63_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1672128.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3025 a         1830 c         2281 g         2811 t
ORIGIN      
        1 agagatactc ttaaatataa gtaaatgatg atttggttag tcctagaacc aatcaactta
       61 tgtttatcgg cttggcagat tttatgtttg tcatttgttt gtatagaggt atttcagaaa
      121 cagaattcta tcaacaacta atcgcttata taggtgtatt ctctgcttgt ttatcacgct
      181 tctgctcatg tggggcttga agtattataa aaagtagctt tctatccttt tctgacagga
      241 tatttgagta gaaggctcta ttaaattgtt agtgaaataa atgaatggag gtatttaata
      301 tgaaatacag attatttttt gttattttct tgagtagtgt gttggatatt cttttaggga
      361 catttttaca aatctctatc gtatctattg ggtggcttgt tctttacagt ggtttgtttg
      421 aagcaggagt tttccttctt gctaataaag gggtggcggt aaaaatcaag gaagtagata
      481 ttcgaaatcg ctttaaattt atttttggaa aaaccttatg gtttcaaatt cttttgctca
      541 tctttttgat aatcaaactt tatcttggtt tggatgcgag gttgatttta ttctatggac
      601 atattttcat tgtctttaat gccttaatgt atctattatc tagtagtcag gttagcctta
      661 aaaaaaacaa actgtcttct taatcttaca gcagtagagc ctgacaaggt gaggttatag
      721 tagggaggat ttctatgaaa aagtatcaac ttctattcaa aataagtgca gtcttctctt
      781 acttattttt cgtatttggt ctttctcagc tgacgcttat cgtccaaaac tattggcaat
      841 tttcttctca gataggcaat ttattctgga ttcaaaatat cttgagttta ctttttattg
      901 gagtcatgat tgtggttctt gttaagacag gccatggtta tctcttccgc attccaagaa
      961 aaaaatggct ttggtattcg attttgacag tattagtggt agtgttccag atctctttta
     1021 acgttcagac agctaaacat gttcagtcaa ctgcggaagg ttgggctgta ttgattggtt
     1081 atagtgggac taactttgca gagctaggta tttatatagc cctgttcttt ctggttccac
     1141 tgatggaaga attgatttat agaggattac tgcaacatgc tttctttaag cattcgcgat
     1201 ttggtcttga tttgcttctt ccttctattt tatttgctct ccctcatttt tcaagcctgc
     1261 ctagtctgtt agatatcttc gtctttgcaa cagttggaat catctttgct ggtttgaccc
     1321 gctataccaa gagcatttat ccatcctatg cggtgcatgt gatcaataat attgtagcga
     1381 ccttcccgtt tttgctcact tttctacata gggtcttggg gtaaaaaaac aaaaggcttg
     1441 cttttcagcc atagaggagg tcatcatgta taaacactta tttttcctag attccaaaac
     1501 tttagatcgg ttgacaccct atattctagt cttggcttct gacaccattg cttttaatgt
     1561 ttttgtgcta acctttgtat ctgcggtggt ttttaatttc ctaaattcca tgctagcttt
     1621 aatggctata ttcatagggg ctggctatgt ggtcggattt tggttactaa tactcaatga
     1681 aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg caaggcgacg
     1741 ttgacgtggt ttgaatttga ttttcgaaga gtataaaatg gtttgttttg gaaaggctag
     1801 aactaaagaa tgacgtgtag gaagtctgtt tgttgaggag gataatttta tggagttttt
     1861 tgataaattt catgccttgt gttttggatt tttagtacta ataattgtca ttacagttcc
     1921 ttatacgatt aaccatgggg gtttttttca aaatgaatct gcattgattc ttgtaagtct
     1981 tcttgtaacc tcgctgagtg ttgcttatgc tagaaagttt gaaatgattt cttttgggat
     2041 gttaagcaag aaacaacttt tgcttttcat tgcaatcttt cttctaagtg tacttgagac
     2101 gctggtttat attcatttct tcgctgtttc ttctggctca ggggtccaac acttggcgga
     2161 agtcagcaga ggaatttccc tgtctttgat tttgactacc tcagtttttg gccccatcca
     2221 ggaggaactc attttcagag gacttcttca aggtgcggtt tttgacaatt cttggttagg
     2281 gcttgtgcta acttcctctc tcttttcttt catgcatgga ccttctaatg tcccttcgtt
     2341 tattttttat ctacttgggg gtttgttgct gggctttgct tataaaaaga gtcaaaacct
     2401 atgggtttct actctagtcc atatgcttta caacagttgg ccactcttat attatttata
     2461 aaaattatga aaaggtaagc agaagacggt gcttaccttt ttcaagctat aatgataccc
     2521 aagccaatcc agaggctttt ctttgagaat cgggttagag tggttgacga ataggccaaa
     2581 aactagtaga atagtaagga aactttatac ggaggaaaga aatggatttg ggtgataatg
     2641 agctaacact gactcccata cctgggaaaa gtggcaaggc ttatatgggt agctatcctg
     2701 atgggaagcg catctttgta aaaatgaaca cctctccaat cctacctggt ctagctagag
     2761 aacaaattgc tccacaatta ttatggagtc gtcgtttggc agatgggcgt gatatgtgtg
     2821 ctcaagaatg gttgacaggc aagatattga ccccctatga tatgaatcgt aagcaaatcg
     2881 tcaatatttt aacccgtctt catcgctcac gtccgttgat gacacaattg agtcgtttgg
     2941 gctatgccat ggaaacacct gtagatttac tacagtcttg gcaggaaacg gctccagatg
     3001 ctttgcgtaa aaatcatttt atcagtgaag tgatggctga tttacgtcag actattccag
     3061 gatttagaga ggaccatgcg accattgtcc atggagatgt acgacatagt aattggattg
     3121 agacagatag tggcttgatt tatttagtag attgggattc ggttcgcttg accgatcgca
     3181 tgtttgatgt ggcccatatg ctctgccatt atatttcaga acatcagtgg aaggaatggt
     3241 tgacctacta cggttacaag tacaatcaaa cggtattaag taaattgtat tggtatggtc
     3301 aattgtctta tttgagtcag atttccaagt attatatgaa ccaagattta gaaaatgtca
     3361 atcgggagat tcatggtttg cgtcatttcc gagacaagta tggaaagaga agatgagagt
     3421 tagaaatcgt aaaggggcaa cagaattact agaggcaaat ccccagtatg tggtcctcaa
     3481 tcccttggaa gccaaggcaa aatggcggga cttgtttggc aatgataatc ccattcatgt
     3541 ggaagttgga agtggaaagg gtgcctttgt ttcaggtatg gccaagcaag accctgacat
     3601 caactatatc gggattgata ttcaaaagtc tgttttgagc tacgctttgg acaaggtgct
     3661 tgaagttgga gtgcctaaca tcaagctctt gtgggtagat ggttctgact taactgacta
     3721 ctttgaagac ggtgagattg atcgcttgta tctgaacttt tcagatccat ggccgaaaaa
     3781 acgccatgaa aagcgtcgtt tgacctacaa gaccttcttg gataccttca aacgtatctt
     3841 gcctgaaaat ggagaaattc atttcaagac ggataaccgt ggcttgtttg agtacagttt
     3901 agtgagcttt tctcaatatg gcatgaaact caatggtgtc tggttagatt tgcatgccag
     3961 tgattttgaa ggcaatgtca tgacagaata cgagcaaaaa ttctcaaaca aggggcaagt
     4021 tatctaccga gttgaggcag aattttaaga gatagcctaa aattaggctg tacaagtgct
     4081 tttgctttac ataagttggc aaacgtgcta tactgatagt aagaatatga aaagtgaggc
     4141 ggggaaatat cttcgcctct tgcttatgag gaggtggacg caatcgcaac aatcgtagaa
     4201 ttagtcagag aagttgtaga acctgtcata gaagctcctt ttgaactcgt ggatatcgag
     4261 tatggaaaga ttggcagtga catgattctc agtatttttg tagataaacc cgaaggaatt
     4321 accttgaacg acacggcaga cttgacagaa attatcagtc ctgtcctaga caccatcaag
     4381 ccagatccct tcccagaaca atatttccta gaaattacca gtccaggttt ggaacgccct
     4441 ttgaaaacca aggatgccgt cgctggagcg gttggaaaat acatccatgt cgggctctac
     4501 caagccatcg ataagcaaaa ggtctttgaa ggaaccttgt tggccttcga agaggacgag
     4561 ttgactatgg aatatatggg caagacgcgt aagaaaaccg tccaaattcc atacagttta
     4621 gtatcaaaag cacgtttagc agttaaatta tagaaaaaga aaggatagct tttgaggatt
     4681 caaaagtgaa gaaaacatga gtaaagaaat gctagaggcc ttccgcattt tggaagaaga
     4741 caagggaatc aaaaaagaag atatcatcga cgcagtagta gagtcgcttc gttccgctta
     4801 tcgcagacgc tatggtcagt cagacagcgt agctattgac ttcaacgaaa aaacaggtga
     4861 ctttacagtt tatactgtcc gtgaagttgt tgatgaagta tttgatagcc gtttggaaat
     4921 cagcttgaaa gatgctcttg ccattaattc agcttatgaa cttggagaca aaatcaagtt
     4981 tgaagaagca ccagctgagt ttggtcgtgt agcagcccaa tctgccaaac aaaccatcat
     5041 ggaaaaaatg cgcaagcaaa cacgtgccat cacttacaat acttacaaag aacatgagca
     5101 agaaatcatg tctggtacag tagaacgctt tgacaaccgc tttatctatg tcaaccttgg
     5161 tagcatcgaa gcccaattgt caaaacaaga ccaaattcct ggagaagttt ttgcttctca
     5221 tgatcgtatc gaagtttatg tttacaaggt tgaagacaac cctcgtggtg tgaacgtctt
     5281 tgttagccgt agccatccag aaatgatcaa acgtttgatg gagcaagaaa ttccagaagt
     5341 ttatgatgga actgttgaaa tcatgagcgt ggctcgtgaa gcaggtgacc gtacgaaggt
     5401 tgctgttcgt agccacaatc caaacgtgga tgctatcggt acaatcgttg gacgtggtgg
     5461 tgctaatatc aagaagatta ctagcaaatt ccacccagct cgttacgatg ctaaaaatga
     5521 ccgcatggta ccaatcgaag aaaatatcga tgttatcgag tgggtagcag atccagctga
     5581 atttatctac aatgccatcg ctcctgctga ggttgaccaa gttatctttg atgaaaacga
     5641 cagcaaacgt gccttggtgg ttgttccaga taacaagctt tctcttgcca ttggtcgtcg
     5701 tggacaaaac gtgcgcttgg cggctcactt gactggttac cgtatcgata tcaagtctgc
     5761 tagcgaattt gaagccatgg aagacgctgc ttcagtagag ttggaagtag aaaacgatac
     5821 tgtagaagaa taaaagctgc tagaggaggg aaagatgaaa acaagaaaaa tccctttgcg
     5881 caagtctgtt gtgtctaacg aagtgattga taagcgtgat ttgctccgca ttgtcaagaa
     5941 caaggaagga caagtcttta ttgatcctac gggcaaggcc aatggccgcg gcgcttatat
     6001 caaactagac aatgcagaag ccctagaggc gaaaaagaag aaggtcttta accgcagctt
     6061 tagcatggaa gtggaagaaa gcttttatga cgagttgatc gcttatgtgg atcacaaagt
     6121 gaaaagaaga gagttgggac ttgaataagc aaaagataag taatctcttg gggcttgctc
     6181 agcgagcagg gcgcatcata tcgggtgaag aattggtggt caaggccatt caagacggca
     6241 aggccaagtt ggtctttcta gctcatgatg ctggacccaa tctgaccaag aagattcaag
     6301 ataaaagtca ttattatcaa gtagaaattg taaccgtgtt ttcaacactg gaattaagca
     6361 tagcagtcgg gaaatcgaga aaggttttgg ctgtaacaga tgctggattt acaaagaaaa
     6421 tgaggtctct tatggaatag aagaggagga catgatttgt ctaagaaaag attgtacgaa
     6481 atcgcaaaag aacttggaaa agaaagtaaa gaagttgtag cgcgtgcaaa agagttgggc
     6541 ttggatgtga aaagccactc atcaagtgtg gaagaagctg tcgctgcaaa aattgctgcc
     6601 agctttaagc ctgcagctgc tccgaaagta gaagcaaaac ctgcagcacc aaaagtaagt
     6661 gcagaaaaga aaaccgaaaa atctgagcca gctaaaccag ctgtagctaa ggaagaggca
     6721 aaaccggctg agccagttgc tccgaaaaca gaaaaagtag cagcgaaacc gcaaagccgt
     6781 aatttcaagg ctgagcgtga agcccgtgct aaagagcagg cagaacgacg caagcaaaat
     6841 aagggcaata accgtgacca acaacaaaac ggaaaccgtc agaaaaacga tggccgtaat
     6901 ggtggaaaac aaggtcaaag caaccgcgac aatcgtcgct ttaatgacca agctaagaag
     6961 cagcaaggtc agcaaaaacg tagaaatgag cgccgtcagc aagaggacaa acgttcaaat
     7021 caagcggctc cacgtattga ctttaaagcc cgtgcagcag ccctaaaagc agagcaaaat
     7081 gcagagtacg ctcgttcaag tgaggaacgc ttcaagcagt atcaggctgc taaagaagcc
     7141 ttggctcaag ctaacaaacg caaggaacca gaggaaatct ttgaagaagc ggctaagtta
     7201 gctgaacaag cacagcaagt tcaagcagtg gttgaagtcg tccctgagaa aaaagaacct
     7261 gcagtggata cacgtcgtaa aaaacaagct cgaccagaca aaaatcgtga cgattatgat
     7321 catgaagaag atggtcctag aaaacaacaa aagaatcgaa gtagtcaaaa tcaagtgaga
     7381 aatcaaaaga atagtaactg gaataacaac aaaaagaaca aaaaaggcaa taacaagaac
     7441 aaccgtaatc agactccaaa acctgttacg gagcgtaaat tccatgaatt gccaacagaa
     7501 tttgaatata cagatggtat gaccgttgcg gaaatcgcaa aacgtatcaa acgtgaacca
     7561 gctgaaattg ttaagaaact tttcatgatg ggtgtcatgg ccacacaaaa ccaatccttg
     7621 gatggggaaa caattgaact cctcatggtg gattacggta tcgaagccaa acaaaaggtt
     7681 gaagtggata atgctgacat cgaacgtttc tttgtcgaag atggttatct caatgaagat
     7741 gaattggttg agcgtccacc agttgttact atcatgggac acgttgacca cggtaaaaca
     7801 acccttttgg atactcttcg taactcacgt gttgcgacag gtgaagcagg tggtattact
     7861 cagcatatcg gtgcctacca aatcgtggaa aatggtaaga agattacctt ccttgataca
     7921 ccaggacacg cggcctttac atcaatgcgt gcgcgtggtg cttctgttac cgatattacg
     7981 atcttggtcg tagcggcaga tgacggggtt atgcctcaga ctatcgaagc cattaaccac
     8041 tcaaaagcgg ccaacgttcc aatcatcgta gccattaaca agattgataa accaggtgct
     8101 aacccagaac gcgttatcgg tgaattggca gagcatggag ttatgtcaac tgcttggggt
     8161 ggagattctg aatttgttga aatctcagct aaattcaacc aaaatatcga agaattgttg
     8221 gaaacagtcc ttcttgtagc tgaaatccaa gaactcaagg cagacccaac agtgcgtgca
     8281 atcggtacgg ttatcgaagc gcgcttggat aaaggaaaag gtgcggtcgc aacccttctt
     8341 gtacaacaag gtaccttgaa tgttcaagac ccaatcgttg tcggaaatac cttcggtcgt
     8401 gtccgtgcta tgaccaacga ccttggtcgt cgtgttaaag ttgctggacc atcaacacca
     8461 gtctctatca caggtttgaa cgaagcaccg atggcgggtg accactttgc cgtttacgag
     8521 gatgaaaaat ctgcgcgtgc agcaggtgaa gagcgtgcca aacgtgccct catgaaacaa
     8581 cgtcaagcta cccaacgtgt tagccttgaa aacctctttg atacccttaa agctggggaa
     8641 ctcaaatctg ttaatgttat catcaaggct gatgtacaag gttctgttga agccctttct
     8701 gcctcacttc aaaagattga cgtggaaggt gtcaaagtga ctatcgtcca ctcagcggtc
     8761 ggtgctatca acgaatcaga cgtgaccctt gccgaagctt caaatgcctt tatcgttggt
     8821 ttcaacgtac gccctacacc acaagctcgt caacaagcag aagctgacga tgtggaaatc
     8881 cgtcttcaca gcattatcta caaggttatc gaagagatgg aagaagctat gaaagggatg
     8941 cttgatccag aatttgaaga aaaagttatt ggtgaagcag ttatccgtga aaccttcaag
     9001 gtgtctaaag tgggaactat cggtggattt atggttatca acggtaaggt tgcccgtgac
     9061 tctaaagtcc gtgttatccg tgatggtgtc gttatctatg atggtgaact cgcaagcttg
     9121 aaacactata aagacgacgt taaggaagtt acaaacggcc gtgaaggtgg attgatgatc
     9181 gacggctaca atgatattaa gatggatgat gtgattgagg cgtatgtcat ggaagaaatc
     9241 aagagataag attttttgct cctttcttag gtggtgaggg acgcaagcaa accgatggtt
     9301 tcattgctta tttttgagcc tagggtctca aaaatcccct gtgatgggac tgataaatca
     9361 gttccatcac tttcaccacg gcgaaagaag cagatgactt caaattgaac ttcgtttcaa
     9421 tttaaactga aaatcaagaa gtttaaaata gctaggtctg ctggcctagc ttttggttca
     9481 aagtagagaa aggaatatca tggcaaatca tttccgtaca gatcgtgtgg gcatggaaat
     9541 caagcgtgaa gtcaatgaga ttttgcaaaa gaaagtccgt gatccacgtg tccaaggtgt
     9601 gaccatcaca gatgttcaga tgctgggtga cttgtctgtt gccaaggttt attacaccat
     9661 tttgagtaac cttgcttcgg ataaccaaaa agcccaaatc gggcttgaaa aagcaactgg
     9721 taccatcaaa cgtgaacttg gtcgcaattt gaaattgtac aaaatcccag atttgacctt
     9781 cgtcaaagac gagtccatcg agtatggaaa caagattgac gagatgctac gcaatctgga
     9841 taagaactaa agaagagggg ttgcccctct tttttggtgg aggaaaatag gttgaatttg
     9901 aaatggaaaa atattctttt aaaatatatt gaaactagaa tagtacg
//
LOCUS       DAVLYR010000064         9663 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLYR010000064 DAVLYR010000000
VERSION     DAVLYR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.064"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            28..1956
                     /gene="glgB"
                     /locus_tag="VYO63_001207"
     CDS             28..1956
                     /gene="glgB"
                     /locus_tag="VYO63_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1672129.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRKDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATNITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            1946..3088
                     /locus_tag="VYO63_001208"
     CDS             1946..3088
                     /locus_tag="VYO63_001208"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1672130.1"
                     /translation="MKNEMLALILAGGQGTRLAKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGIDSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLELGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3078..4217
                     /gene="glgD"
                     /locus_tag="VYO63_001209"
     CDS             3078..4217
                     /gene="glgD"
                     /locus_tag="VYO63_001209"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1672131.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4214..5647
                     /gene="glgA"
                     /locus_tag="VYO63_001210"
     CDS             4214..5647
                     /gene="glgA"
                     /locus_tag="VYO63_001210"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1672132.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5706..6077
                     /locus_tag="VYO63_001211"
     CDS             5706..6077
                     /locus_tag="VYO63_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1672133.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVPGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            6117..6350
                     /locus_tag="VYO63_001212"
     CDS             6117..6350
                     /locus_tag="VYO63_001212"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1672134.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAELG
                     IAFCSKEMLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            6359..7475
                     /locus_tag="VYO63_001213"
                     /pseudo
     CDS             6359..7475
                     /locus_tag="VYO63_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(7472..7918)
                     /locus_tag="VYO63_001214"
     CDS             complement(7472..7918)
                     /locus_tag="VYO63_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1672135.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            8082..9386
                     /gene="eno"
                     /locus_tag="VYO63_001215"
     CDS             8082..9386
                     /gene="eno"
                     /locus_tag="VYO63_001215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1672136.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFLGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
BASE COUNT         2882 a         1738 c         2146 g         2897 t
ORIGIN      
        1 aaaacgctta caggagaaag ggagtatatg gataatagag aagcattaaa aacctttatg
       61 acgggtgaaa atttttatct ccaacattat ctaggagcac atagggaaga actaaatgga
      121 gagtatggct ataccttccg tgtttgggca cctaatgctc aggctgttca cttggttggt
      181 gattttacca attggattga aaatcagatt ccaatggtaa gaaaggactt tggggtctgg
      241 gaagtcttta ccaatatggc tcaagaaggg catatttaca aatatcatgt cacacgtcaa
      301 aatggtcatc aactgatgaa gattgaccct tttgctgtca ggtatgaggc tcgtccagga
      361 acaggggcaa tcgtaacaga gcttcctgat aagaaatgga gggatggact ttggctggca
      421 cgaagaaaac gttggggctt tgctgaacgt cctgtcaata tttacgaggt tcatgctggc
      481 tcttggaaga gaaatcctga tggcagtcct tatagttttg cccaattgaa agatgaactc
      541 attccttacc tagttgagat gaactatact catattgagt ttatgccctt gatgtcccac
      601 ccactaggtt tgagttgggg gtaccagctt atgggttact tcgctttaga gcatgcctat
      661 ggtagaccag aggagtttca agattttgtc gaggagtgtc atacccataa tattggggtt
      721 attgtggact gggtaccagg tcactttacc atcaatgatg atgccttagc ctattatgat
      781 gggacaccga cttttgaata ccaagaccat aataaggctc ataaccatgg ttggggtgcc
      841 cttaattttg accttggaaa aaatgaagtc cagtccttct taatttcttg cattaagcat
      901 tggattgatg tctatcattt ggatggtatt cgtgtggatg ctgttagcaa catgctctat
      961 ttggactatg atgatgctcc atggacacct aataaagatg gcggaaatct caactatgaa
     1021 ggttattatt tccttcagcg cttgaatgag gttattaagt tagaatatcc agatgtgatg
     1081 atgattgcag aagaaagttc gtctgcgacc aatattacgg gaatgaaaga gattggtggt
     1141 ctaggatttg actacaaatg gaacatgggc tggatgaatg atatcctccg tttctacgaa
     1201 gaagatccga tctatcgtaa gtatgacttt aacctggtga ctttcagctt tatgtatgtt
     1261 ttcaaggaga attatctctt gccattctcg cacgatgaag tggttcatgg caagaagagt
     1321 atgatgcata agatgtgggg agatcgttac aatcaattcg caggcttgcg caatctctat
     1381 acgtaccaaa tttgtcaccc tggtaagaaa ttgctcttca tgggtagcga atacggtcaa
     1441 ttcctagaat ggaaatctga agaacagttg gaatggtcta acctagaaga cccaatgaat
     1501 gctaagatga agtatttcac ttctcagcta aaccagtttt acaaagatca tcgctgtctg
     1561 tgggaaattg ataccagcta tgatggtatt gaaatcattg atgcggataa tcgagaccag
     1621 agtgttcttt cctttattcg taagggtaaa aagggagaaa tgttagtctg tatctttaat
     1681 atggtacctg ttgagcggaa agattttaca atcggactac ccgttgcagg aatttacgaa
     1741 gaagtatgga atactgagtt ggaagagtgg ggaggcgttt ggaaagaaca taatcaaact
     1801 gttcaaacgc aagaaggact atggaaagat tatgagcaga ccttaacctt taccctaccg
     1861 gctatgggag caagtgtatg gaaaatcaaa cgtcgcttga aatctactaa aaccgtcaca
     1921 aataaaaacc aaaaaggagt agaaaatgaa aaatgaaatg ttagctttga ttcttgctgg
     1981 tgggcaagga actcgtctcg ctaaactcac tcaaagcatc gcaaaaccag ctgtgcaatt
     2041 tggtgggcgc taccgtatca ttgactttgc cctatcaaac tgtgccaact cagggattca
     2101 caatgttggg gtcgttacac agtatcaacc acttgctctc aacaaccata ttgggaatgg
     2161 ttcaagctgg ggactagacg gtattgattc aggtgtctct attcttcaac cttattctgc
     2221 aagtgaagga aatcgttggt ttgaggggac tagtcacgct atttaccaaa atatcgacta
     2281 tatcgacagt gtcaatcctg agtatgtctt gattttgtct ggggatcaca tctacaaaat
     2341 ggactatgat gatatgctcc agtctcataa ggataataat gccagcttga cagtagcagt
     2401 tttagacgtc cctcttaaag aagcaagccg ttttggtatc atgaacacag atgctaacaa
     2461 tcgtattgtt gaatttgaag aaaaaccagc tcaacctaaa tctacaaaag cttctatggg
     2521 aatctacatt tttgattggc aacgccttcg taatatgtta gtcgctgctg aaaagagcaa
     2581 ggttggcatg tcagactttg gtaaaaatgt cattccaaat taccttgagt taggtgaaag
     2641 tgtttatgct tacgaattta gtggttattg gaaagatgtt ggtactattg agtcactttg
     2701 ggaagcgaac atggagtata tttctccaga aaatgccttg gatagtcgta accgtcaatg
     2761 gaagatttac tcaagaaact tgatttcacc accaaacttc ctcggggcaa atgctcatgt
     2821 ggaagactca ttagttgtag acggatgttt cgttgatgga actgttaaac attctatcct
     2881 ttcaacaggc gcgcaagttc gcgaaggagc ggaagtcctt gattcagtta tcatgagtgg
     2941 agctatcatt ggtcaaggag ctaagattaa acgtgccatt attggtgaag gtgcgattat
     3001 ttctgacggt gtcgaaattg atggaacaga tgaagtacaa gttgtaggat ataatgaagt
     3061 agtgggggta gcaacagatg aagattgata aatattctgc cattttagga aatacagttg
     3121 gttttcacaa tatgtcgaca ttgacggacc accgtccagt agcaagtttg ccatttggtg
     3181 ggaaatatcg tttgattgat ttcccacttt caagccttgc taatgcaggt gttcgtagtg
     3241 tctttggtat tttccagcag gataatatca gctcagtatt tgaccatatt cgttcaggac
     3301 gcgagtgggg cttatcaacc cttcttagcc attactatct aggaatttac aatacccgtg
     3361 tagaaagtag tacagttgga aaagaatact accaacagct tcttacttat ttgaaacgtt
     3421 ctggctcaaa ccaaacggtt gccctcaact gcgatgttct tattaacatt gatttgaacc
     3481 aagttttcca cctacatagt acaacaaaag agcctatcac tgtagtttat aaaaaactag
     3541 ctaagaaaga tatttcagaa gtaaatgcaa tcttggatgt agatgaaaca gaccatgttc
     3601 tttctcataa actctttgat agcaagtcaa cagctgaaac gttcaatatg tctacagata
     3661 tctttgtcgt tgatacacct tggttgattg aacacttgga agaagaagct aaaaaagaac
     3721 atccagagaa attgcgctat gttttacggg atttggctgt aaaagaggga gctttcgcct
     3781 acgagtacac gggctatcta gccaatattc actctgttaa atcttattat caagcgaata
     3841 tcgatatgct tgaatcacaa aaattctatt ctcttttctc accaaaccaa aagatttata
     3901 caaaggtcaa aaacgaagag ccaacttact atgctaatac atctaaggta agcacttctc
     3961 agtttgcctc tggtagtatt attgaaggtc aagtagctaa ttctgttcta tcacgtaata
     4021 ttcatgtcca taaggatagc ttggttaaag atagcctgct cttccctcgt gttgttattg
     4081 gagaaggggc tcaggtcgaa tatgctatct tggacaaagg ggttgaagtt gagcctggtg
     4141 ttgtgattcg aggaactgca gaacatccag ttgtggttaa gaaaggtgct aaagtaacag
     4201 aggatattca ttcatgaaaa ttttatttgt agcagcagag ggtgcaccct tttcaaaaac
     4261 aggtggtttg ggagacgtca ttggcgctct tccaaaatca ctggtaaaag ctgggcacga
     4321 agttgcagtg attttaccct actatgatat ggtagaggct aaatttggaa atcagattga
     4381 agatgtgctt cattttgagg tgagcgttgg ttggcgcaga cagtattgtg gaattaagaa
     4441 aacagtatta aatggtgtaa ccttctactt tattgacaat caatattatt tcttccgtgg
     4501 tcatgtttac ggtgattttg atgacggaga acgctttgcc tttttccaac tggctgccat
     4561 tgaggctatg gaaaggattg actttattcc tgatcttctc catgttcatg actaccatac
     4621 agctatgatt cctttcttgt tgaaggaaaa ataccgttgg attcaagcct atgaggacat
     4681 tgaaacagtt ttaaccattc ataatttaga attccaagga caattttcag aaggaatgtt
     4741 gggtgatttg tttggagttg gctttgaacg ttacgctgat ggcacccttc gatggaacaa
     4801 ctgtctgaac tggatgaagg caggtattct ctatgcgaac cgtgtttcaa ccgtttcacc
     4861 tagctatgct catgaaatta tgactagtca gtttggatgt aatttggatc agattcttaa
     4921 aatggagtct ggtaaagtat ctggtatcgt gaatgggatt gatgctgatc tttataatcc
     4981 tcagacggat gctcttttag actatcattt caatcaggaa gatttgtctg ggaaagccaa
     5041 aaataaggca aaattgcaag aaagagttgg cttgcctgtt agagcagacg ttccactggt
     5101 gggaattgtt tctcgtttga cacgtcaaaa aggttttgat gtggtggtcg aaagtcttca
     5161 ccatatcttg caagaagatg ttcagattgt tcttttggga actggcgatc cagcctttga
     5221 aggagctttc tcatggtttg ctcagattta cccagacaag ctatcaacaa atatcacttt
     5281 tgatgtcaaa cttgctcagg aaatctacgc tgcttgtgac ctcttcctca tgccaagtcg
     5341 ttttgaaccg tgtggcttgt ctcaaatgat ggctatgcgt tatggaacct tgccattggt
     5401 ccatgaagtt ggaggcttgc gagatacagt tcgcgctttc aatccaatcg aaggaagcgg
     5461 tactggcttt agctttgaca atctatctcc ttattggtta aattggactt tccaaacagc
     5521 attggacttg tatagaaacc atccagacat ttggagaaac ctacaaaaac aagctatgga
     5581 gagtgacttc tcatgggata cagcctgcaa gtcatacctt gacttgtacc atagtttagt
     5641 taattaatag ataaaatcgt atgatgactt catacgattt ttaggctgtt tagagaggag
     5701 aactgatgtc tcaagtaaaa ggcttgtgtg ttttggatgt agatggaacc ttaatcctag
     5761 aagaagtgat tgatctttta gggagagagg caggtcatga ggcggaaatt tcgcagatta
     5821 caagtcgagc aatgcgagga gagttggtct ttgaaagcag tttaagaaaa agagtgtcct
     5881 tgttggaagg tcttcctatt ttggtctttg ataacgtctt caactcaatt catctatcgc
     5941 taaatgtccc agagttcatc tctattctcc aaaagaatgg catcctagtt ggtctggtgc
     6001 ctggtggatt tacaccaata gttggagaga ttagcaaaat cccttggtat tgcctatttc
     6061 actgccaacc agcttgaagt caaagaaggt ttttaacagg aaaattagtt ggacaaatta
     6121 taagtcccca ggtcaaaaaa gaaactctgg aaaaatggag aaagaaacta aaactttcta
     6181 aagaaagaac ggtggcaatc ggtgatgggg tcaataatct attaatgttg aagtcggcgg
     6241 agttaggaat cgccttttgt tccaaagaaa tgctcaaaaa agaaatacca catcatgttg
     6301 acaagaggga ttttttagaa gttcttcctt tgattgactg tttagaatga gggaaaatat
     6361 gaagattgta attgcaccgg attcgtttaa ggaaagcttg acagctcaac aggtagctga
     6421 agcaataaaa agaggcttcc aacaatcgat agcagatgta gaatatctcc tctgccctgt
     6481 tggtgatggg ggagaaggta ctgtagatgc tatccgacat tctcttgacc taaaagaaaa
     6541 atggatccaa gtgacagacc cttttggaca aaaagaagcc atgcgctatt ttcaaaaagg
     6601 ggaactggca ctatttgaag tagctgactt ggttggtctt ggaaaaattc cgctagagaa
     6661 acgaaatcca cttcaaatcc aaacttgtgg tattggagag ttgattctcc atctcatttc
     6721 taaagggatt aaagatattt atatcggtgt tggtagcacg gccagtaatg atggaggact
     6781 ggggattgct gctggtttag gttatcaatt ttatgatagg gatggaaatg tcttgcctgc
     6841 ttccagtcaa tccttattaa acttagcttc tgtttcaaca gaaaattgct ataaaattcc
     6901 tgaaggtgtt caaattcata ttctagcaga tgtcgtgagt cccttatgtg gtcatcaagg
     6961 tgcgacttac acttttggca atcaaaaagg tctacatccg actatgtttg cagtcgtaga
     7021 tcaggcgatc caagattttt atgaaaaatt ctcacctgca acattagaaa ttaaaggagc
     7081 aggagcaggt ggaggccttg ctggtggttt gtgtgccttt gctcaggcaa gtatcgtgtc
     7141 tggaattgat accttgcttg gacttaatca actttgataa gaaagtttca gatgctgact
     7201 tgcttgttgt tggagaagga agacttgaca gtcaaagctt tgctgggaaa gcgcctatag
     7261 gtgttgcaaa aagaacccct gttggagttc ctgttattgc tatttgcggt agtcttgctg
     7321 aagatttacc tcccctacca tttgaaaata tccaagctgt cttttctatt ttggagaaaa
     7381 gtgaaccttt agaagatagt ttgaaaaatg ccagtctcta tctggagcgc acggctgcca
     7441 atattgggca cttattaaat atgtgcaaga tttagctaaa ccatttcttc cataaggatg
     7501 ttttggctgg ttcttccttt ttaacttccc aaagttttgg aaaagcaagt cgaaggtctt
     7561 tttcttctac ttgaactggt gcattgctat gaataaccag gccaaaagga gaagtaatat
     7621 gctcttcaga tactatggtg gcttgactat cagtttcttt tgcttctttt aagtagaaaa
     7681 cttgcttatc aaattcgata gttggtgaaa tcttcacaaa tagtggctct gctttttcct
     7741 gaaggttttc taaaataaat aaaaagcctt tttctaactg agggctattt gctgtgtcaa
     7801 tatctacata tccaagaact ctttcctcaa aagtaccaag atagcgtctt tgctcatccg
     7861 gatttatttt taacccacca tgagcttttt caagtaattg ttttgataaa tctgtcatga
     7921 aaacagtata tcataaaagt tagaataaaa cagacaaaag aaagcgatta ctttataaag
     7981 ttaaggaaaa gttgcacaaa gataacgttt tttcttgaaa aaggaggggt tttaaggtat
     8041 tatagaggtg taaaaaattt aactcataag gagagtaaaa aatgtcaatt attactgatg
     8101 tttacgctcg cgaagtccta gactcacgcg gtaacccaac acttgaagta gaagtttaca
     8161 ctgaatcagg tgctttcgga cgtggtatgg ttccatcagg agcttctact ggtgaacacg
     8221 aagcagttga acttcgcgac ggtgacaaat ctcgttacgg tggtcttggt acacaaaaag
     8281 ctgttgacaa cgtaaacaac atcattgctg aagctatcat tggctacgat gtacgtgatc
     8341 aacaagctat tgaccgtgct atgatcgcac ttgacggtac tcctaacaaa ggtaaattgg
     8401 gtgcgaatgc aatcctcggt gtgtctatcg ctgtagctcg tgctgctgct gactaccttg
     8461 aaatcccact ttacagctac cttggtggat tcaacactaa agttcttcca actccaatga
     8521 tgaacatcat caacggtggt tctcactctg acgctccaat cgctttccaa gagttcatga
     8581 tcttgccagt tggtgcgcca acatttaaag aagcccttcg ttacggtgct gaaatcttcc
     8641 acgctcttaa gaaaatcctt aaatcacgtg gtttggaaac tgccgtaggt gacgaaggtg
     8701 gattcgctcc tcgtttcgaa ggaactgaag atggtgttga aactatcctt gctgcgattg
     8761 aagctgctgg atatgtacca ggtaaagacg tatttctcgg atttgactgt gcttcatcag
     8821 aattctacga taaagaacgt aaagtttacg actacactaa atttgaaggc gaaggtgctg
     8881 ctgttcgtac atctgcagaa caaatcgact accttgaaga attggttaac aaatacccaa
     8941 tcatcactat tgaagatggt atggatgaaa acgactggga tggttggaaa gctcttactg
     9001 aacgtcttgg taagaaagta caacttgttg gtgacgactt cttcgtaaca aacactgact
     9061 accttgcacg tggtatccaa gaaggtgctg ctaactcaat ccttatcaaa gttaaccaaa
     9121 tcggtactct tactgaaact tttgaagcta tcgaaatggc taaagaagct ggttacactg
     9181 ctgttgtatc acaccgttca ggtgaaactg aagattcaac aatcgctgat atcgcagttg
     9241 caactaacgc aggacaaatc aagactggtt cactttcacg tacagaccgt atcgctaaat
     9301 acaaccaatt gcttcgtatc gaagaccaac ttggtgaagt agctgaatat cgtggattga
     9361 aatcattcta caaccttaaa aaataaaata gttcagtgaa ctattttatc ccgaaccatg
     9421 aaattcaaaa gttcggccgt tgatttaaca acgtttctag cccctcggat tttatccgaa
     9481 gggctatttt tgtatttaga gggcaaaaaa gggacaaaac tttttggctc tttgtcaact
     9541 gtagtgggtt gaagtcagct aagctcgaga aaggacaaaa ttcgtccttt cttttttgat
     9601 gttcagagcg ataaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt
     9661 gcg
//
LOCUS       DAVLYR010000065         9464 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLYR010000065 DAVLYR010000000
VERSION     DAVLYR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.065"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            44..1717
                     /locus_tag="VYO63_001216"
     CDS             44..1717
                     /locus_tag="VYO63_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1672137.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            1829..2920
                     /gene="ulaG"
                     /locus_tag="VYO63_001217"
     CDS             1829..2920
                     /gene="ulaG"
                     /locus_tag="VYO63_001217"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1672138.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            3034..5010
                     /gene="tkt"
                     /locus_tag="VYO63_001218"
     CDS             3034..5010
                     /gene="tkt"
                     /locus_tag="VYO63_001218"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1672139.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            5127..5426
                     /gene="yajC"
                     /locus_tag="VYO63_001219"
     CDS             5127..5426
                     /gene="yajC"
                     /locus_tag="VYO63_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1672140.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            5473..5901
                     /locus_tag="VYO63_001220"
     CDS             5473..5901
                     /locus_tag="VYO63_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1672141.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEILYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            5903..6313
                     /locus_tag="VYO63_001221"
     CDS             5903..6313
                     /locus_tag="VYO63_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672142.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            6614..9265
                     /gene="adhE"
                     /locus_tag="VYO63_001222"
                     /gene_synonym="adhC"
     CDS             6614..9265
                     /gene="adhE"
                     /locus_tag="VYO63_001222"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1672143.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         3027 a         1748 c         1975 g         2714 t
ORIGIN      
        1 aaagaattgc tgtgatatca atagatttgg gggatttttt aatatggtac tggataaggc
       61 aagttgtgat ttgcttcaat atttgatgga tcaagaaacg tccaaaacga ttatggcgat
      121 ttcgaaagat ttgaaagagt caagaaggaa aatttattat cacattgaca aaatcaatgc
      181 tgctctgggt gacgaggcgc ttcacatcat tagtattcca cgaattggta ttcacttaac
      241 ggaagagcag agagatgctt gttgtaaact attatcggaa gtagattcgt acgattatat
      301 catgagtgcg catgaacgta tgatgataat gttactatgg ataggtattt ctaaagaacg
      361 tattacgatt gaaaaattga tagagttaac agaggtatct aggaatactg ttctcaatga
      421 tttgaatagt attcgttatc aactaacttt ggaacaatat caggtgatct tgcaagtgag
      481 caagtcacag ggataccacc ttcatgccca ccctcttaat aaaattcagt atcttcaatc
      541 gcttctatat catattttta tggaagaaaa tgccactttt gtatctattt tagaagataa
      601 gatgaaagag aggttagatg atgagtgttt gctttctgtt gaaatgaacc aattttttaa
      661 ggaacaggtt cctttagttg aacaagattt agggaagaaa ataaaccatc atgaaataac
      721 ttttatgttg caggttctac cttatttgct gttaagctgt cataatgttg aacagtatca
      781 agaaagacat caggatatag agaaagaatt ttctttgata agaaaaagaa tagagtatca
      841 ggtgtctaag aaattaggag aacggttgtt tcaaaagttt gaaatttctt tgtcaggact
      901 tgaagtttct cttgtagctg ttctcctcct ctcctatcgt aaagatttgg atattcatgc
      961 agaaagtgat gattttcggc aattaaaact tgctttagaa gaatttatct ggtattttga
     1021 atcacaaatc cgaatggaga ttgagaacaa ggatgatttg ttacgaaatt tgatgatcca
     1081 ctgtaaagcc ttgttattta gaaagactta cggtattttt tctaaaaatc ctctaacaaa
     1141 acaaattcga tccaagtatg gagaattatt tttagtcact agaaaatctg cggaaatttt
     1201 agaaggagca tggtttattc ggctaacaga cgatgatatt gcctatttga cgattcatat
     1261 tggaggattt ttaaaatata caccatcgtc tcaaaaaaat atgaaaaaag tttatctcgt
     1321 ttgtgatgaa ggtgttgcgg tttcgagact tttgctgaaa caatgcaaac tttattttcc
     1381 aaatgagcaa attgacactg tatttacaac agaacaattt aagagtgtgg aagatattgc
     1441 acaagttgat gtagtgatta ctactaatga tgatttggat agcagatttc cgattttaag
     1501 ggttaatcct atccttgaag cagaagatat tttgaaaatg ctagactatc ttaaacacaa
     1561 tatatttcgt aataagagca aaagtttcag tgaaaatctt tctagtctta tttcgtctta
     1621 tattgtagac agcaagttgg ctagtaagtt ccaagaagag gttcaaacac ttataaatca
     1681 agaaatagta gttcaagctt ttttggaaga tatttgaagg acagtccaat gatgaacaca
     1741 aacctgtgtt tttcttggtc ttttttagtg ttttgaaggg tggtatacta atctcaaaga
     1801 taacaattat atccaaagga ggcaacatat gccaaacgtc aaagaaatta caagagagtc
     1861 atggatttta gccactttcc cagagtgggg aacatggttg aacgaagaaa tcgaagaaga
     1921 agtcgtacct gaaggcaact ttgccatgtg gtggctaggc aactgtggta cttggattaa
     1981 gacaccagct ggtgctaacg ttgtcatgga cctttggtca aaccgtggaa aatcaaccaa
     2041 aaaagtgaaa gatatggttc gtgggcacca aatggcaaat atggcaggtg ttcgtaagtt
     2101 gcaaccaaac ttgcgtgttc agccaatggt tatcgatcca tttgctatca acgaactaga
     2161 ctattactta gtttcacact tccacagtga tcatatcgac ccatacacag ctgcagcaat
     2221 tctcaacaat cctaagttag agcatgttaa gtttatcggt ccttaccact gtggacgaat
     2281 ctgggaagga tggggtgttc caaaagaacg tatcatcgtt gttaaaccag gtgacactat
     2341 cgaattaaaa gatatgaaga ttcatgcagt agaatcattt gaccgtactt gcttggtaac
     2401 tctcccagtg aacggtgctg atgagacagg cggtgaactt gctggcttgg ctgttacaga
     2461 tgaagaaatg gctcaaaagg ctgttaacta tatctttgaa acaccaggtg gaaccatcta
     2521 tcatggtgca gattctcact tctcaaacta ttttgcaaaa catggtaaag actttaaaat
     2581 tgatgttgct ttgaataact atggtgaaaa tccggtaggt atccaagaca aaatgacatc
     2641 tatcgacctt cttcgtatgg cagaaaatct gcgtaccaaa gtcattatcc cagttcacta
     2701 tgatatctgg tctaacttca tggcttctac taatgagatt ctagaacttt ggaaaatgcg
     2761 aaaagatcgc ttgcaatacg atttccatcc atttatctgg gaagttggcg gtaagtacac
     2821 ttatcctcaa gatcaacact tagtagaata ccatcatcca cgtggttttg atgattgttt
     2881 tgaacaagac tctaacattc aatttaaagc tttgctataa tataaaaata ttctttggag
     2941 gttatccgta tgttgcatga tacggataat tttcatataa tagtaaaata gaatgtgtga
     3001 ttcaataatc acctcaaata gaaaggaaat tctatgtcaa atctatctgt taatgcaatt
     3061 cgttttctag gtattgacgc cattaataaa gccaactcag gtcatccagg tgtggttatg
     3121 ggagcggctc cgatggctta cagcctcttt acaaaacaac ttcatatcaa tccagctcaa
     3181 ccaaactgga ttaaccgcga ccgctttatt ctttcagcag gtcatggttc aatgctcctt
     3241 tatgctcttc ttcacctttc tggttttgaa gatgtcagca tggatgagat taagagtttc
     3301 cgtcaatggg gttcaaaaac accaggtcac ccagaatttg gtcatacggc agggattgat
     3361 gctacgacag gtcctctagg gcaaggtatt tcaactgcta ctggttttgc ccaagcagaa
     3421 cgtttcttgg cagccaaata taaccgtgaa ggctacaata tctttgacca ctatacttac
     3481 gttatctgtg gagacggaga cttgatggaa ggtgtctcaa gcgaggcagc ttcatacgca
     3541 ggtttgcaaa aacttgataa gttggttgtt ctttatgatt caaatgatat caacttggat
     3601 ggtgagacaa aggattcctt tacagaaagt gttcgtgacc gttacaatgc ctacggttgg
     3661 catactgcct tggttgaaaa tggaacagac ttggaagcca tccatgctgc tatcgaaaca
     3721 gcaaaagctt caggcaagcc atctttgatt gaagtgaaga cggttattgg atacggttct
     3781 ccaaacaaac aaggaactaa tgctgtacac ggcgcccctc ttggagcaga tgaaactgca
     3841 tcaactcgtc aagccctcgg ttgggactac gaaccatttg aaattccaga acaagtatat
     3901 gctgatttca aagaacatgt tgcagaccgt ggcgcatcag cttatcaagc ttggactaaa
     3961 ttagttgcag attataaaga agctcatcca gaactggctg cagaagtaga agccatcatc
     4021 gacggacgtg atccagtcga agtgactcca gcagacttcc cagctttaga aaatggtttt
     4081 tctcaagcaa ctcgtaattc aagtcaagat gccttgaatg ttgtggcagc taaattacca
     4141 acctttctag gtggatcagc tgaccttgct cactcaaaca tgacttatat caagacggat
     4201 ggacttcaag acgatgccaa tcgcttgaat cgtaacattc agtttggtgt tcgtgaattt
     4261 gcaatgggaa caatcttgaa cgggatggcc cttcatggtg gacttcgtgt atacggtgga
     4321 actttcttcg tcttctctga ctatgtgaag gcagctgtcc gcttgtcagc cttacaagga
     4381 cttcctgtga cttatgtctt tacccatgat tcaatcgcag ttggggaaga tggtccgact
     4441 cacgaaccag ttgagcattt agcaggtctt cgtgctatgc caaatctaaa tgttttccgt
     4501 ccagcagatg cgcgtgaaac gcaagcagct tggtaccttg cagtgacaag tgagaaaaca
     4561 ccaactgccc ttgtcttgac acgtcaaaac ttgactgttg aagatggaac agacttcgac
     4621 aaggttgcta aaggtgctta tgttgtatat gaaaatgcag ccgactttga taccatcttg
     4681 attgcgacag gttcagaggt taatcttgct gtctcagctg ctaaagaatt ggctagtcaa
     4741 ggcgaaaaaa tccgcgtagt cagcatgcca tctacagatg tctttgataa acaagatgca
     4801 gcttacaagg aagaaattct tccaaatgca gtccgccgtc gtgttgcagt cgaaatgggt
     4861 gcaagtcaaa actggtacaa atatgttggt ctcgatggtg ccgttctagg tattgatacc
     4921 ttcggagcct ctgccccagc accaaaagta ttggcagaat atggctttac tgtagaaaat
     4981 cttgtaaaaa ttgttcgaaa cttgaaataa tcctaaaaat cagggcgtaa gctctggttt
     5041 ttcttaccag aaaagtaagg tacaatcttg taaaagtagc tgaaatttga tatagtagtc
     5101 ctatgtaaaa gacaaaggag aaaacaatga atccaaatat tactttttta atcatgcttg
     5161 taggtatgat ggccttgatg ttctttatgc aacgttctca aaagaaacaa gctcaaaagc
     5221 gtatggaaag cttaaataaa ctacaaaaag gctatgaagt gattacaatc ggtggacttt
     5281 atggaacagt cgatgaagta gatacggaga agggaacaat agttcttgac gtagatggag
     5341 tttacttgac ttttgaacta gctgctatca agacagtatt accgctgaaa gaaacagctt
     5401 cactagaagg cgcaattgaa aaataagacg ggatcacgaa ctcccgtttt tctataaaag
     5461 aaaggaaatg ggatgaaaaa attagtcttt gtctgtctgg gaaatatttg ccgtagccct
     5521 atggccgagt ttgttatgaa atcaatgaca gataactacg aaatccaaag tcgagcaact
     5581 tcctcttggg aacatggcaa tccgattcat aaggggactc agggaatttt tcaacagtat
     5641 gagattcttt atgacaagaa caagacatcg cttcagatta gtaaggaaga ttttgaagcc
     5701 tttgattata ttatcggaat ggacgcttca aatatttctg acttacgtca gatgtgtcca
     5761 gtagactgtc aagataagat ttactcattt tcatctgaaa gtgttccaga cccttggtat
     5821 acaggagatt ttgaagaaac ctatcgacgt gttcaagagg gctgtcaagc ttggttagaa
     5881 cgtttagaaa aggagagtta aggtggaaaa tcttacgaat ttttacgaaa agtatcgtgt
     5941 ctatctgact cgtccacgtt tagagctttt ggcagtagtt accattgttt tctgtgctgt
     6001 actcgtcttt tttctaaata ttccaggaaa aggtgtctta aaactcgata atggaacgat
     6061 tgtttatgat ggcagtcttg tccgtggtaa aatgaatggt caaggtacca ttaccttcca
     6121 aaatggagac caatatacag gtggcttcaa caatggagcc ttcaacggaa aaggtacctt
     6181 tcaatctaaa gaaggctgga cctacgaagg tgattttgta aatggtcagg ctgaaggaaa
     6241 agggaaacta acaacagaac aagaagtcgt ttatgaagga acttttaaac aaggcgtttt
     6301 tcaacaaaaa taaagcctcc ttatccaaag gaggtattat tagaaccaca aggtaagcgc
     6361 ttacctgtaa atccctttct ttccaaatcc ctcttccaag caagtttgtg aaataaaaaa
     6421 tatttgaaat aaatttcaca aacttcaaag ataaaacctg ataagaaaag aaaatgagaa
     6481 aagtttcaca agagtttaaa aattctttgt gaaatgttta tgaaactgtt tacaaagttg
     6541 aaataatgcg ttataataaa cttgtgaaaa aattaacaaa ggatatcgtt ccttgaaagc
     6601 tatggaggaa aatatggctg ataaaaaaac tgtgacacca gaggaaaaga aactcgttgc
     6661 tgaaaaacac gtagatgagt tggttcaaaa agctctagtt gcccttgaag aaatgcgtaa
     6721 attggatcaa gagcaagttg actacatcgt tgctaaagca tcagtagcag ctttggatgc
     6781 ccacggagaa ttggctttac atgcctttga agaaacagga cgtggtgtat ttgaagataa
     6841 agcaactaag aacttgtttg cctgtgaaca cgtagtaaac aacatgcgtc acactaagac
     6901 agttggcgtt atcgaagaag acgatgtaac aggattgact cttattgctg aaccagttgg
     6961 tgttgtttgt ggtattactc caacaacaaa cccaacatca acagcaatct tcaaatcatt
     7021 gatttcattg aagacacgta acccaatcgt ctttgccttc catccatcag cacaagaatc
     7081 atctgctcat gcagctcgta tcgtccgcga tgcagctatc gcagctggtg ctcctgaaaa
     7141 ctgtgtgcaa tggattactc aaccatctat ggaagcaaca agtgccctta tgaaccacga
     7201 aggtgttgcg acaatccttg caacaggtgg taatgccatg gttaaggcgg cttattcatg
     7261 tggtaaacca gctcttgggg taggtgccgg aaacgttcca gcttatgttg aaaaatcagc
     7321 aaacattcgt caagcagcac acgatatcgt catgtctaaa tcatttgata acggtatggt
     7381 ctgtgcatct gaacaagcag ttatcattga taaagaaatt tacgatgaat ttgtagcaga
     7441 gttcaaatct taccacactt actttgtaaa caaaaaagaa aaagctcttc ttgaagagtt
     7501 ctgcttcggc gtcaaagcaa acagcaaaaa ctgtgctggt gcaaaattga acgctgacat
     7561 cgttggtaaa ccagcaactt ggattgcaga acaagcagga tttacagttc cagaaggaac
     7621 aaacattctt gctgcagaat gtaaagaagt tggcgaaaat gagccattga ctcgtgaaaa
     7681 attgtcacca gttattgcag ttttgaaatc tgaaagccgt gaagatggta ttactaaggc
     7741 tcgtcaaatg gttgaattta acggtcttgg acactcagca gctatccaca cagctgacga
     7801 agaattgact aaagaatttg gtaaagctgt taaagctatt cgtgttatct gtaactcacc
     7861 ttctactttt ggtggtattg gggacgttta caatgccttc ttgccatcat tgacacttgg
     7921 atgtggttct tacggacgca actcagttgg ggataacgtt agtgccatta acctcttgaa
     7981 tatcaaaaaa gtcggaagac ggagaaataa catgcaatgg atgaaacttc cttcaaaaac
     8041 atactttgaa cgtgattcaa ttcaatacct tcaaaaatgt cgtgacgttg aacgtgtcat
     8101 gatcgttact gaccatgcca tggtagagct tggtttcctt gatcgtatca tcgaacaact
     8161 ggaccttcgt cgcaataagg ttgtttacca aatctttgcg gatgtagaac cggatccaga
     8221 tatcacaact gtaaaccgtg gtactgagat tatgcgtgcc ttcaaaccag ataccatcat
     8281 cgcactcggt ggtgggtctc caatggatgc tgccaaagta atgtggctct tctacgagca
     8341 accagaagtg gacttccgtg accttgtcca aaaattcatg gatatccgta aacgtgcctt
     8401 caagttccca ttgcttggta agaagactaa attcatcgcg attccaacta catctggtac
     8461 aggatctgaa gtaacaccat ttgccgttat ctctgataaa gcaaacaacc gtaaataccc
     8521 aatcgctgac tactcattga caccaactgt ggcaatcgta gatcctgctt tggtattgac
     8581 agttccagga tttgttgctg ctgatactgg tatggacgta ttgactcacg cgacagaagc
     8641 atacgtatca caaatggcta gtgactacac tgatggttta gcacttcaag ccattaaatt
     8701 ggtctttgaa aatctcgaaa gctcagttaa gaatgcagac ttccactcac gtgagaaaat
     8761 gcataacgct tcaacaatcg ctggtatggc ctttgccaat gccttcctag gtatttctca
     8821 ctcaatggcc cataagattg gtgcgcaatt ccacacaatc cacggtcgta caaatgccat
     8881 cttgcttcca tacgttatcc gttacaacgg tacacgtcca gctaagacag caacatggcc
     8941 taagtacaac tactaccgtg cagatgaaaa ataccaagat atcgcacgca tgcttggact
     9001 tccagcttct actccagaag aaggggttga atcttacgca aaagctgtct acgaactcgg
     9061 tgaacgtatt gggatccaaa tgaattttag agaccaagga attgacgaaa aagaatggaa
     9121 agaacattct cgtgaattag ccttcctggc ttatgaagac caatgttcac cagctaaccc
     9181 acgtcttcca atggtagacc atatgcaaga aatcatcgaa gatgcatact atggctacaa
     9241 agaaagacca ggacgccgta aataattgtt tatcagtcta gaagcaagac aaaaactcaa
     9301 tttgagggaa agatccagta atttttctat gataaaaggc atcctatcaa ggtttttgaa
     9361 cacctgatag gatgcctttt tatgatattg aggccttttt gccctttttg aaaaactaga
     9421 atagaaacaa aatatataat agattgaaac tagaatagta caca
//
LOCUS       DAVLYR010000066         9288 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLYR010000066 DAVLYR010000000
VERSION     DAVLYR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.066"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(151..771)
                     /locus_tag="VYO63_001223"
     CDS             complement(151..771)
                     /locus_tag="VYO63_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1672144.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(771..1253)
                     /locus_tag="VYO63_001224"
     CDS             complement(771..1253)
                     /locus_tag="VYO63_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1672145.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1253..2095)
                     /locus_tag="VYO63_001225"
     CDS             complement(1253..2095)
                     /locus_tag="VYO63_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1672146.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2190..2990)
                     /locus_tag="VYO63_001226"
     CDS             complement(2190..2990)
                     /locus_tag="VYO63_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1672147.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3134..4513
                     /gene="glmU"
                     /locus_tag="VYO63_001227"
     CDS             3134..4513
                     /gene="glmU"
                     /locus_tag="VYO63_001227"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1672148.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4523..5068
                     /locus_tag="VYO63_001228"
     CDS             4523..5068
                     /locus_tag="VYO63_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1672149.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5080..5394
                     /locus_tag="VYO63_001229"
     CDS             5080..5394
                     /locus_tag="VYO63_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1672150.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAAIFLLILLVLAMK
                     LL"
     gene            5411..6103
                     /locus_tag="VYO63_001230"
     CDS             5411..6103
                     /locus_tag="VYO63_001230"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1672151.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6180..6671
                     /gene="rocS"
                     /locus_tag="VYO63_001231"
     CDS             6180..6671
                     /gene="rocS"
                     /locus_tag="VYO63_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1672152.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            6769..7359
                     /locus_tag="VYO63_001232"
     CDS             6769..7359
                     /locus_tag="VYO63_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1672153.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAD
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7368..9131
                     /locus_tag="VYO63_001233"
     CDS             7368..9131
                     /locus_tag="VYO63_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1672154.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2856 a         1628 c         1998 g         2806 t
ORIGIN      
        1 attttgctct acaatcctgt tgagaagaca cgtgttcata tcaaaaaggt attggcaagt
       61 tgcaatacct ttttacaagg ttcttttgtc ttatttttgt ttcaactgac tatatctcct
      121 atggttctag ttcagaaggc taggctataa ttatgattga taagaagtat cattccaagt
      181 attgagagtg aatgtttcaa aatcatgggt ttctataatg gtcaggctgg catttgctag
      241 accgccatct ttacgaagaa gtggttcttt atagcctagg agagtacgaa gactggcagt
      301 aagattggcg ccgtgtccga caattagaat acgctcagct ggactatctt ttaatgattt
      361 gataaattgg atggtccgtt gagttgtact atagagggat tcggctccga acattcgagt
      421 gtcaaattga gcaagatttg aacgaaaagc ctggatttgt tgcgggtaaa tagcttccaa
      481 ggttgcaatt ttcaaacctt ctaacttccc aagttgccat tcacggagat taggaacgat
      541 ttctaaagaa cagggggtat agagttgact ttggataatc tcagcagatt tgaccgctcg
      601 aggtaaatca cttgaataaa tctgatcaaa aggaatttcc ttgagatact ggccaagtcg
      661 ttttagggtt tcaatggatt caggaagaag aggagaatca ccgctagcac cttgaaaacg
      721 accttcttgg ttccagaggg tacgaccgtg gcggacaaag tagagtttca ttacttgatg
      781 tcctccaaaa tatctacaaa gtctgccttt acaaagctag ccaagtcttg tggcgcgacg
      841 ataatgctgt gtccgacttc gcctgcagag acaatcattc gatccaaatc tagagcaatt
      901 ttatcgataa aaatgggata attgtgtttc tgacgaattc cgacaggatt attggctcca
      961 tgaatgtaac cagttgtttt ttctaagtcc ttttgtggaa tcatgctcac ttttttattg
     1021 ccagaaattt tagctagttt cttttcagac aagtgctgag tgatagggac aattccgata
     1081 atcggtccgg tcttgtctcc caaaagcgcc aaggttttga aaatctgatc tcgttcataa
     1141 ccttgaggaa gctctccttc tagggcattg atttgaatcc cctgatgagg gatagctgct
     1201 ttagatagga tttgttccac caatgttttt ttgattttaa ctttttttgc cattatttat
     1261 atttatcctc caattgactc atccaaatac caagccagat tcccagcgca aagaagaagg
     1321 cgatgatgac ataaccgaca agtgaaagtc ctgtgtattg gatactttca gcgtttcctg
     1381 catttggaat taagatcaaa agggtacttg ataggacgat accgatgatg aaatgataga
     1441 cgcgtgagtg gtagttgttt aaggcataat ccatcaattt tgaaaaaacg atgagagttg
     1501 cacctgcacc aattccaatc ggaaagaagg ttcccaagag atcaaaagtt ttaaaaccag
     1561 tcaacatagg agcatagagt cccaaaatca aaagtaagtt cgatgggctg aggccaggaa
     1621 ccaagacgcc aagggccaat agtgcgcctg ctaggacgaa gttaagaaag ctggcgctta
     1681 aggttccaac gacaaaattt aaggcataga gtcctaatcc agaaatgata aaggttgtcc
     1741 ataaccaagc taaatcaatc ttgtctcggt cagattctcg agttgattct ttgaggaggc
     1801 taggaactgt accgataata gctcccgcaa agctccataa tacaaagacc tgataatttt
     1861 caagcaggta ttcaatcggg taggaaaata agccgattcc cagaagcata ccgatggcaa
     1921 ctggaataaa gtacaaaaca ttttctttaa ggtctttaaa gggatgggcc agaaagccaa
     1981 tcattcgttc atagattcct aagattgctg ctagaacccc tccggaaatt cccggtagga
     2041 taaatccaag agcaattaca atccctttaa taacgcgtgc taaccatgag agcatatatt
     2101 tcctccaact atttctattt caaaaaatcc ttactatatt gtatcacaat cagacacaaa
     2161 aagaaaaagc aaatgataaa caaatgcttt taaggtttta aaaaaagttt tcgaaaggtt
     2221 tcttctttat tttttaaggg agagataacg ttgatatcta aatcgtggtc aaagccggca
     2281 atttttcctt tagatgtgta ttggtgaata tcataatcta aatcagtttt aggactgctc
     2341 tccaaaaatc ctgagtctga gccgtaggac ggaatccaaa cagaggtaaa cttgcctgta
     2401 tcaatactgt gttcttccat gaagtagaca ccaacgtaga tgccgatgtt tttagcacct
     2461 agtgatgcta gttttgctcg aaagttttcg acaccttcgt tcatattaga catggttttg
     2521 tcttccacgt caagccaata gtaactaggg ctgtaaggag aggcagcatt gtagaaaact
     2581 tcggcagcct tttccatttc ttggacactt tttccagcta cataagcgta gacagcaact
     2641 gggacattcc gcttttgaag ttcagtgata tgactcttat aggccttgtc tattccattg
     2701 ataaatgaag catcattttc ttttgttgtt tgagcaccac tgtgaacacg aacaatagca
     2761 cctgaaatat tttgtgagag ggcatcgtag ttgatttcct caggacgctg ccagccagag
     2821 aggtcaataa tcggtttgtc taagtgtttc aaagcctgtg cttcaatctg tgctatattg
     2881 gattttgttt taaacgattg gctgtcatta agtgggcgat tgatgattaa aatgaacatc
     2941 ataatcccaa aaaaactaaa taaaataagt ggatgaattt gttttctcat atcttataag
     3001 tctaccctaa aaatcaaaaa aaatcaaaaa aatgggttaa ggaagagact ttagagcatt
     3061 ttttcattca agagtgcgga atgatttgaa atatggtata ataaaaggga atttctacag
     3121 aaaagagaag attatgtcaa attttgccat tattttagca gcgggtaaag ggactcgcat
     3181 gaaatctgat ttgccaaaag ttttgcacaa ggttgcgggt atttctatgt tggaacatgt
     3241 tttccgtagt gtgggagcta tccaacctga aaagacagta acagttgtag gacacaaggc
     3301 agaattggtt gaggaggtct tggctggaca gacagaattt gtgactcaat ctgaacagtt
     3361 gggaactggt catgcagtta tgatgacaga acctatctta gaaggtttgt caggacacac
     3421 cttggtcatt gcaggagata ctcctttaat cactggtgaa agcttgaaaa acttgattga
     3481 tttccatatc aatcataaaa atgtggccac tatcttgact gctgaaacgg ataatccttt
     3541 tggctatgga cgaattgttc gtaatgacaa tgctgaggtt cttcgtattg ttgagcagaa
     3601 ggatgctaca gattttgaaa agcaaatcaa ggaaatcaac actggaacat acgtctttga
     3661 caacgagcgt ttgtttgagg ctttgaaaaa tatcaatacc aataacgctc aaggcgaata
     3721 ctatattaca gacgtcattg gtattttccg tgaaactggt gaaaaagttg gcgcttatac
     3781 tttgaaagat tttgatgaaa gtcttggggt aaatgaccgt gtggcgcttg cgacagctga
     3841 gtcagttatg cgtcgtcgca tcaatcataa acacatggtc aacggtgtta gctttgtcaa
     3901 tccagaagca acttatatcg atattgatgt tgagattgct ccggaagttc aaatcgaagc
     3961 caatgttacc ttgaaagggc aaacgaaaat tggtgctgag actgttttga caaacggtac
     4021 ttatgtagtg gacagcacta tcggagcagg agcggtcatt accaattcta tgattgagga
     4081 aagtagtgtt gcagacggtg tgacagtcgg tccttatgct cacattcgtc caaattcaag
     4141 tctgggtgcc caagttcata ttggtaactt tgttgaggtg aaaggatctt caatcggtga
     4201 gaataccaag gctggtcatt tgacttatat cggaaactgt gaagtgggaa gcaacgttaa
     4261 tttcggtgct ggaactatta cagtcaacta tgacggcaaa aacaaataca agacagtcat
     4321 tggagacaat gtctttgttg gttcaaattc aaccattatt gcaccagtag aacttggtga
     4381 caattccctc gttggtgctg gttcaactat tactaaagac gtgccagcag atgctattgc
     4441 tattggtcgc ggtcgtcaga tcaataaaga cgaatatgca acacgtcttc ctcatcatcc
     4501 taagaaccag taggagccta tcatggagtt tgaagaaaaa acgcttagcc gaaaagaaat
     4561 ctatcaagga ccaatattta aactggtcca agatcaggtt gaattaccag aaggcaaggg
     4621 aactgcccga cgggatttga ttttccacaa tggggctgtc tgtgttttag cagtaacgga
     4681 tgaacaaaaa cttatcttgg tcaagcagta ccgcaaagct atcgaggctg tctcttacga
     4741 aattccagcc ggaaaattgg aagtaggaga aaacacagcc cctgtggcag ctgcccttcg
     4801 tgaattagag gaagaaacag cctatacagg gaaattagaa ctcttgtacg atttttattc
     4861 agctattggc ttttgtaatg agaagttaaa actatattta gcaagcgatt tgacaaaagt
     4921 ggaaaatccg cgtccgcagg atgaggatga aaccttggaa gtccttgaag tgagcttaga
     4981 agaagcgaaa gaattaatcc aatcaggtca tatctgtgat gccaagacaa ttatggctgt
     5041 tcagtattgg gagttgcaga aaaaatagag gaggtcagta tgggtaaatc tttattaacg
     5101 gatgaaatga ttgaaagagc taatagaggc gaaaaaattt caggtcctcc tttgctagat
     5161 gataatgagg aaactaagat tttaccaacc tcttcttccc gttttggtta tgccaatcct
     5221 aaggatcatg gttttagcca ggaaaccttg aagattcagg tcgaaccatc tattcataaa
     5281 agccgtcgta ttgaaaatac caagagaaat gtcttcaatt ctaagttgaa taaaatctta
     5341 tttgcggcca tctttctctt gattttgctt gttttagcaa tgaaactttt gtaatagaaa
     5401 aggaattgaa atgaaaatag gaattattgc tgctatgcca gaagaactgg cttatctggt
     5461 ccagcattta gataataccc aggagcaagt tgttttgggg aatacctatc atacaggaac
     5521 cattgcttct catgaagtcg ttcttgtaga aagtggaatt ggtaaggtca tgtctgctat
     5581 gagtgtggcg attttggctg atcatttcca ggtggatgcc cttattaata cgggttcagc
     5641 tggggcagta gcagaaggta tcgctgttgg ggatgtcgtg attgctgaca aattagccta
     5701 tcatgacgtg gatgtcacag cttttggcta tgcttatgga caaatggcgc aacaaccgct
     5761 ttatttcgaa tcagacaaaa cctttgttgc tcaaatccaa gagagtttat ctcaattgga
     5821 ccaaaactgg catcttggtt tgattgctac aggagatagt tttgttgcag gaaatgacaa
     5881 gatagaagcg attaagtccc atttcccaga agttttagcc gtggagatgg agggggcagc
     5941 tattgctcaa gcagcgcatg ccctcaatct cccagtctta gtcatcagag ctatgagtga
     6001 caatgccaac catgaagcaa acatcttttt tgatgagttt attatcgaag ctggacgtcg
     6061 ctctgcccaa gtcttgttga cctttttgaa ggctttagat taagcggaaa tttgacagtt
     6121 tttctagctt atgataagat ttaagtaaag aaaagctaga aaacgtttca gaggatatta
     6181 tgagtattga aatgaccgtc agtgagattg cagaggtctt aggattatct cgccaagcaa
     6241 tcaataaccg tgtcaaagaa ttaccagaag aagacacaga taaaaatgac aaaggggtaa
     6301 cagttgttac cagaagtggc ttgattaagc tagaagaaat ctataaaaaa acgatttttg
     6361 aagatgagcc tgtcagtgaa gatgtcaaac aacgtgaact gatggagatt ctagtggatg
     6421 agaagaatgc agaaattctt cgtctttatg aacaattaaa agccaaggat cgtcagttat
     6481 cagaaaaaga cgagcagatg cgtatcaaag accgtcagat tgctgagaaa gacaaacaat
     6541 tagatcagca acaacaattg acccttcaag ccatgaagga tcaagaaaat cttaagctag
     6601 agctggacca agcaaaagaa gaagtccaat ccactaagaa aggctttttt gctcgtttat
     6661 ttggaggata atcaaggagc tgtttaggtt aaatgctaac ctgatggctt cttttgtttg
     6721 tgaatagtat agttgattag aacagagaca gtagaggaga ggtacagaat ggaacgatta
     6781 gaagattaca ttgcttttga cttagaattt aatcagcacg aaggacaaac acacttgatt
     6841 caagtatcgg cagtgcgttt tagagatggt cgggaaatcg atgcctatga ttcctatgtt
     6901 cataccagcg tccctctaaa aagctttatc aatggtttga ccgggattac agctgagacc
     6961 ttaaaagatg ctcctccagt tgagaaagtc ttagaagaat tccaagagtt tgtgggttct
     7021 ttgctgatga taggctacaa tgctgctaaa agtgatttac ctattttagc agatcatggt
     7081 ttagactata gccaacagta caaggtggat ttgtatgatg aagcttttga acgtcggagt
     7141 tcagatttac atggtattgc caatctcaaa ttgcaaactg ttgcctcatt tcttggattt
     7201 caaggtcagt ctcataatag tttagaggat gctcggatga cggcgcgtgt ttacgaatcc
     7261 ttcttagagt cggatcaagc tagggaattg ttagagacag aaagtagctt atcaaacaat
     7321 ccttttggtg tcttggactt gtctcaatta tttgactagg agtgcctatg aaacctgaaa
     7381 aacctaaaaa attgggtctt aaacagattt acctcaatct tgataaaatc ctctttctat
     7441 ttttcttgat ttttatgata gtggagtatt tgtgggtacc attcaattca tggttggcta
     7501 gcttcttatt agatcagaca ggttatcttt ttatttccta taacaatgtc ttagccatcc
     7561 ttactcattc tccattgatt agtctagctt ttttagcctt gattgtaatt aaccttctgg
     7621 ttgcttattt tcaaatagga ctcctcttta ttggtgctcg tcatctcctc tataatgaaa
     7681 agcgaaggct aattgagtat atccgcaagg ttttccatga aagttttgga tttatgaaaa
     7741 agctaacttg cgctaaagcc ttgtttatct ttttctatat agctatgctt tttcctttta
     7801 tacggaaaat gctgaagatt tattatctta ataaaatcgt cattccagag tttatccaag
     7861 cttatttaga agataaatat tggatgtggt ggctgagcat cctacttcta tctcttatct
     7921 ttctctatgt ctctgtcaga ttgatgtttg cccttccgaa gattgtatat gatcagctga
     7981 ctgttcgaga agcagtgatg tttagcttgg agaaaaccaa gaagagagtt actttttatg
     8041 cttggaattt attttacatc ttgctcaaag cacatttatt attttatctt cctttgattc
     8101 ctttattatt ggctcaaact ttggtagatg atatcactca gagagagtct ctgattcttg
     8161 gtattttgaa ttttgtcgtg attaaaaatc tctattatat ggctctgact tattttttgg
     8221 ttaaatttgt ttcatttttg acaggaaagg agctggatat gcttcctagg agagaaaaag
     8281 atcacatcgt gcgatggggt gtcatgactt gtgccagtct tttctttgcc ttagaaggtt
     8341 atatttatct ggaggctccc atggttcatc tacctcaact tatttctcac cgaggggttt
     8401 ccaatgcaaa tgggattcaa aatacagtag agtccttgga aactacagca caactcaaac
     8461 cagacttggt ggagacggac gtgcaggaaa caaaagatgg gcagtttgtc atgatgcatg
     8521 atgctaactt gaaaaatcta gcaggtatca ataaaagtcc tcaagactta aacttggagg
     8581 agcttaaagg gattgatatt tttgaaaatg gctaccagac taaaatttca agctttgaag
     8641 attatctcag tcgagccaac gaacttggtc aaaaattact aattgaaatt aaaaccagta
     8701 aaaaagatag tccagacatg atgaaccgct ttttagcccg ttatgctgca aagctcaaga
     8761 tttatggaca tcaaatccag tctttagact accatgttgt cgaaaaagta agacagtatg
     8821 atgccgaact gccagtttat ttcatcatgc cctacaattc tgtctttcct aaaacaagag
     8881 cgacaggata tactatggag tactcaacct tggatgaata ttttgtaagc aaactatgga
     8941 caacggatca gaaactttat gtctggactg tcaatgattc agaagctatc agcaaatctc
     9001 ttcacttagg agtagatgga gtgattacag atgatttgga aaaagttcag cgggaaatag
     9061 aagtagccca agaagatcca gaatatacgg atttgctctt gaaaaaagca ttggaattct
     9121 ttgaatttta gtaataaaaa agaggaacgg ttgttcctcc catgataggt aagtattctt
     9181 gtgaatgctt gcttttttgt ggttgcaatc cgtttacgag aattgtttgt attgttcttg
     9241 tttcaattta ctttatagtg ctttgaagtt ggaatgctac actataac
//
LOCUS       DAVLYR010000067         9250 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLYR010000067 DAVLYR010000000
VERSION     DAVLYR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.067"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            6..1247
                     /locus_tag="VYO63_001234"
     CDS             6..1247
                     /locus_tag="VYO63_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1672155.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            complement(1478..2890)
                     /gene="sufB"
                     /locus_tag="VYO63_001235"
     CDS             complement(1478..2890)
                     /gene="sufB"
                     /locus_tag="VYO63_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1672156.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(2944..3384)
                     /locus_tag="VYO63_001236"
     CDS             complement(2944..3384)
                     /locus_tag="VYO63_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1672157.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(3371..4597)
                     /locus_tag="VYO63_001237"
     CDS             complement(3371..4597)
                     /locus_tag="VYO63_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1672158.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATAFYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVINPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(4608..5870)
                     /gene="sufD"
                     /locus_tag="VYO63_001238"
     CDS             complement(4608..5870)
                     /gene="sufD"
                     /locus_tag="VYO63_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1672159.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(5898..6668)
                     /gene="sufC"
                     /locus_tag="VYO63_001239"
     CDS             complement(5898..6668)
                     /gene="sufC"
                     /locus_tag="VYO63_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1672160.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(6818..7012)
                     /locus_tag="VYO63_001240"
     CDS             complement(6818..7012)
                     /locus_tag="VYO63_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1672161.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(7040..8695)
                     /gene="dnaX"
                     /locus_tag="VYO63_001241"
     CDS             complement(7040..8695)
                     /gene="dnaX"
                     /locus_tag="VYO63_001241"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1672162.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATDKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(8695..9192)
                     /locus_tag="VYO63_001242"
     CDS             complement(8695..9192)
                     /locus_tag="VYO63_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1672163.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
BASE COUNT         2703 a         2072 c         1739 g         2736 t
ORIGIN      
        1 ctactatgaa aaaaatattt ttaactttgt taactgtctc tcttttaggg ggtgcttcta
       61 ctgctgttgc tcaagatttt accattgccg ctaaacatgc gattgctgtt gaggcaaata
      121 ctggtaaaat tctctatgag aaggatgcaa cgcaacctgt cgaaattgct tccataacaa
      181 aattgattac tgtttatctg gtctatgaag ctttggaaaa cggaagtatt accctctcca
      241 ctcctgtaga tatttctgat tatccttatc aattgacgac aaattctgaa gccagtaata
      301 ttcctatgga ggcccgtaat tatactgtcg aagagttgct tgaagcaact ctggtatcta
      361 gtgccaacag cgccgctatt gccctagctg agaaaattgc tggctcagaa aaagatttcg
      421 tcgatatgat gcgggcaaaa ctcttggaat ggggaattca ggatgccact gttgtcaata
      481 cgacaggtct taacaatgaa actctagggg ataacattta cccaggttct aaaaaagatg
      541 aggaaaataa gcttagtgct tatgatgtcg ctatcgttgc tcgcaacctc atcaaaaaat
      601 acccacaagt cttagaaatc accaaaaaac cttcttctac ttttgctggg atgacaatca
      661 cttcaaccaa ctacatgtta gaaggtatgc ctgcttaccg tggtggtttt gatgggctaa
      721 aaacaggaac aacagataag gctggagagt cttttgttgg tactactgtc gaaaaaggca
      781 tgagagtcat cacagttgtt ttaaatgcag atcatcaaga caataatcct tacgctcgat
      841 ttacagctac atcttcccta atggattata tttcttctac atttacactt cgcaaaatcg
      901 ttcaacaagg cgatgcctat caagatagca aaacccctgt acaagatgga aaagaagata
      961 cagtaactgc agtggctcca gaggatatct atctaatcga acgtgttggg aatcaatctt
     1021 cccaatctgt tcaattcaca cctgattcca aagcaatccc agcaccactt gaagctggaa
     1081 cagtggttgg ccatttgact tatgaagaca aggacttgat tggtcaaggt tacatcacca
     1141 cagagcgccc tagtttcgaa atggtagcag acaagaaaat tgaaaaagcc ttcttcttaa
     1201 aagtttggtg gaatcagttt gtccgctttg ttaacgagaa attataaaaa atcgcgattt
     1261 taaaccgcga ttttttgata caagttcatc ttcaaaatcc ttgattttga gtaaatctaa
     1321 tactcaaaat caaagaacaa actaggaagc tagccgcagc ttgctcaaaa cactgttttg
     1381 aggttgtaga tgaaactgac gaagtcagta accatacata cggtaaggcg atgctgacgt
     1441 ggtttgaaga gattttcgaa gagtataaaa tcaaatttta tccaactgat ccctccattt
     1501 catagctaat caagcggttc agctcaactg cgtattccat tggaagttct tttgtaaagg
     1561 gttctacaaa tcccatgaca atcatttcag ttgcctcaga ttctgacaat ccacggctca
     1621 tgagataata caattgctct tctgaaatct tagatacttt ggcttcgtgt tccaaagcca
     1681 cttgcgagtt gtgaatttca ttaaatggaa tagtatctga tgctgacaag tcatccatga
     1741 taatggtatc acattcaatg tgggaaacag atttcttaga gttcttgtta aaggtgactt
     1801 gtccacggta gtcaaccttt cctccacctt tagcgatgga tttagacaca atagacgagc
     1861 tggtatgtgg agcattgtga atcatcttag cacccgtgtc ttggtgttgc cctgcattag
     1921 caaaggcgat agagagcatg gtaccacgcg ctccttctcc atcaaggtaa acagatggat
     1981 atttcatagt cgttttggca cccaagtttc catcaatcca ctcaacagtg gcatcctttt
     2041 gagccttagc acgctttgtt accaagttat agacgttatc agaccagttt tggatagttg
     2101 tataacgcat ataagctccg tccaaagcaa aaatttctac aatggcagcg tgtaagctat
     2161 tgcttgaata tgttggtgct gtacatcctt ctacgtagtg gacgcttgct ccctcatcaa
     2221 cgataatcaa ggtacgttcg aactgaccta tattttcgtt attgatacgg aaataagttt
     2281 gaagtggaat atctaccttg acaccttttg gcacgtagat aaaagttcca cccgaccata
     2341 ctgctgagtt gagggctgcc aacttgttat ctgtcggcgg taccaacttc gcaaagtatt
     2401 gtttaaataa gtctgggtat tccttgagtg cggaatctgt atctgtaaag ataataccta
     2461 atttttggaa ctcttccttc atgttgtggt aaaccacttc tgactcgtac tgggcagaag
     2521 cccctgctaa ataagcacgt tcagcttctg gaatcccgat acgttcaaag gtttctttaa
     2581 tcttttcagg tacatcatcc caagaacggg ctggtttgtc agatggtttt tggtagtaga
     2641 ttaagtcatc aaagtcaatc tctgacaagt ctgctcccca agtttgcatg ggcatttttt
     2701 tgaaggtttc ataagacttc aaacggaact ccaacatcca ctcaggctca cccttagcag
     2761 cagataattc acgaataaca ccttcgttga gtccttttcc tgtcgataag acaggctcta
     2821 catcgtcatg gaaaccaaat ttatattcac caaggtcaat tggttttggt tctactcttt
     2881 cttcagccat agtatccttt ctttcttcat ttctaataat tcataagaca aaagaaactt
     2941 gtcttactgt ttttcttgat tttcaattgt tttcttaagg gcattccaag ctagggttgc
     3001 acacttgatt ctttgaggga atttggcaac acctgacaag aatgccgcgt ctccaagttg
     3061 gccttgacgc tcatcttttt gcccttgaac catttcagaa aaaatagtcg ccagttctaa
     3121 aatttcttgt ttggtttttc ctaaaacggc atctgtcatc atactagcag aagcagttga
     3181 aatcgtgcat cctgaattta gaaaagcaat atcttccaaa cggtcctctg catcaaactt
     3241 gacagagagg ttgatgacat ccccacaagt cggattgttg agactgattt gctcagcatc
     3301 ttctaacttc ccttggtgat gtggattttt cgaatggtct gctaccactg ccatataaag
     3361 gctatctagt ttagaaagtg ccattgaaaa actcctttgt cttttgtagg gcatcgacta
     3421 gtttgtcgca atctgccttg gtattgtaga tataaaaact tgcacgagct gttgctggga
     3481 cttccaaata ctgaagcaag ggttgcgcac aatggtgacc agcacgaaca gccactcctt
     3541 cataatccag agccgtcgca agatcgtgag gatggagatc acctaggtta aaggcaataa
     3601 cacccgaacg ttgagccaaa tcctgagaac cgtaaatggt caatccctca attgcctgca
     3661 gttttggata gacgtacgca atcaattcct gttcatgagc ttcaacggca tccataccaa
     3721 tcttttccaa ataatcaact gcagtcgcaa gtccaatagc tcctgccata tttggcgttc
     3781 cagcctcaaa tttccaaggc aattccttcc aactagcaaa ttgctcgtag acaaaatcaa
     3841 tcatctcgcc gccaaattct actggagaca tttgctcaag atacttttct ttgccgtaaa
     3901 ggacaccgat accagtcgga ccagccatct tgtgacccga aaaggcgaaa aagtccagat
     3961 ccaagtcctg gacatcaatc ttcatatgag gtgtagattg agcaccatcc actaccatga
     4021 ttgccccaac ttggtgggct aattgagtga tttccttgat cggattgatc acaccaagaa
     4081 cattggaggc atgagctagg gaaacaaatt taaccttatc agtcaattta gctcgcaaat
     4141 cctccatatc caaggcaccg tctttaagat agacatagac aagctctgct ccagtctttc
     4201 gacaagcttc ctgccatgga atgatattag aatggtgttc cattactgaa atcaagacct
     4261 ggtctccctc agtgagaatt tcctcagcaa agcgtgccac ccagttaagg ctggttgtcg
     4321 ttcctctggt aaagagaact tcctttgtag agcctgcatt aataaactta cgaatggttt
     4381 cacgagcagc ttcataaaaa gctgtcgctc gttccgctaa ggtatggaca ccacggtgaa
     4441 cattggcatt gtcctgctca tagtaactgt taatagcttt cagaactact aggggttttt
     4501 gtgtcgtcgc agcattgtcc agatagacca gaggttcatc attgacaatc tgatctaaaa
     4561 ttggaaaatc cttgcgaatc gcttctacat ctaacatagg cttcccctta gcgttttgac
     4621 aatttctctt cgatagttgc aatcatttca tcacgaactt ccttgactgg aatctccacg
     4681 ataacagatc caaggaaacc acgaacaacc aaacgctctg cagttgcctt atccaatcca
     4741 cgactcatga ggtaatacat gtcttctgga tcaacttgtc cgatagacgc tgcgtgtcct
     4801 gcagtgacat cattttcatc aatcaaaaga attgggttag catctgaacg cgcttggtct
     4861 gaaagcatga gaacacggct ctcttgttgc gcatctgctc ccttagcacc cttgatgatg
     4921 tggccgatac cattgaaagt caaagttgct ttttcaagga taaccccatg ttgtaggata
     4981 tttccgatag agttgcagcc atagttagtt acacgagtat caatcccttg tacctgacga
     5041 ccacttgaaa gagctacaac cttgaggtca gcatggctac cattaccaat caagtcacta
     5101 tcaaaatcag caacgacatt tccttcgttc atgacaccga tagcccagtc aatacttgca
     5161 tcgttgccta atttaccacg acggctaatg taggcagtga cgttttcacc tagacggtcg
     5221 atagcagcaa acttgacttg cgcaccagaa cgtgcaatca cttccactgt gatattggca
     5281 gttactttgt cacttccttc accgcgtgac tctaaacgct ccagataact aatcttagaa
     5341 tttttaccag cgataatcat aatatgcttg ttaaacggca cattgctatc gctatcttgg
     5401 tagaaaattc cttcaattgg ctctgtgatt tctacgttat ctggaatata gagtacagca
     5461 ccactgttaa agtaagctgt gtggtaagcc gccaacttgt catcatcata cttaacagat
     5521 gacatgaaga attcttcgat cagctctgga atttcttcta aagctgagtg aaagtctgtg
     5581 aagacaacac cctgttcagc taactcaact ggagtttgtt cgaaaacagt ttgagttcct
     5641 acttgcacca acttcaagtg atgatctaaa gctgtgaaat ctggaacatt tgctgatggc
     5701 tcattttctg taatcgttcc atcacccaga ttccaacggt ggaatttgac acgctcaata
     5761 actggtaatt ccaaagtctc aatcttgtca aaagcttttt gacggagatc agccaaccag
     5821 cttggttcag cgtgcatttc tgaaaaaagt ttaatatttt ctctagtcat ttacttctcc
     5881 taaaagatac gaggaaatta caattcttcc ttgtagtcgt agccaagttc ttcagctaat
     5941 tttgcgtatc cttcacgttc caaacgcgca gccaattctg gaccaccaga aaggacaaca
     6001 cgaccttcca tcatcacgtg taccacatca ggtgtgatat agttcaaaag acgttggtag
     6061 tgagtgatga tcatagcacc aaaaccttca ccacgcatgg cattgacacc tttagacaca
     6121 actttaagag cgtcaatatc aagacctgag tcaatctcgt ccaaaagagc aaatgttggc
     6181 tccaacatca aaagttgaag aatttcattg cgttttttct caccaccaga gaagccttcg
     6241 ttgaggtaac gctctgccat ttcttctttc atgttgagca attccatttt ttcatctagc
     6301 ttagtaataa actcacgaac tgaaatcttc tcatcatctt ctttacccgc attcatagcg
     6361 gcacgaagaa actcagcatt ggtaattcca gggatttctg atgggtattg catagcaagg
     6421 aaaagtccca tacgcgcacg ctcatccact tccaactcaa ggatgtttac gccatcaaac
     6481 aaaacttcac ctttagttac ttcatagttt ggatttccca tgatagcggc agaaagagtc
     6541 gatttacctg taccatttgg tcccatgata gcggcaattt ctcctgtttt cagggtcagg
     6601 ttaacccctt ttaaaatttc ttttccttca atctcaacgt gaagatcttt aatctctaat
     6661 actgacatga tagttccttt ctttttcaaa acttttacag tacttactag aaaaacgtct
     6721 gatttcccca gaaatagagc aaaaattcta taaatatact tttctagtat aacaaaaaaa
     6781 agcctaaaag gctttgattt tgtctggaag ctgagggcta gtctttccta ttaaaatgct
     6841 gatcgatgac atcaacgatt ttgcccatca cataactgac tcggaaattt ctaaagagta
     6901 aaattgccca aaaggctgcc ataatatagc ggccagtcat ccaggcttca ttgaaaaaat
     6961 aggtctcagc agctgtaaac agcgccataa taataaattg ttgtttattc ataaacttat
     7021 tgtatcatga aatctttctt tagtcttcct ctaccttcac tttatcagcc aaaaattcaa
     7081 atccttctgg aatcaggctt tcttctactt ctttttcagt ttgagaagat ttggctttgg
     7141 ctgaaaaggc tgcgcgaact tctttccatt cctccatgga aatagctaaa atctcaggtg
     7201 aaaaacctgc cgcctgactg aggatattac caaacatggt attgagattg tctcgtttca
     7261 tagtttgacc agcattgaag ttagactcaa aagcaagaat agcatggtgt tcattggcag
     7321 caaccggttg agaaccaact agcagagcct tgtccggccc acctagactt tcaattacct
     7381 ctccccaggc attctgcaaa cgaattagat tttgacgtgc taaatcagga ttttcgacgg
     7441 cctcttgtaa gatagattgc actttattgc gatcgacacg atagactgtt ttgtccgtag
     7501 ctggtcgact aggagctggt gcaacttgtt taggaaccgc acctacatta gaaagctctt
     7561 gtttgagacg ggcaacttcc tgtctcagcg tagcaatttc attttcaacc gctcctgata
     7621 gagctggttc gggcttgatt tccgccaaac ggacggtcat catttcagca taaatcttgg
     7681 gctgcaaact agacttaata tctgctaaac tcactgttgc taagcgaatc atttcaaaca
     7741 gatttttttg aggaagtgcc aaattttcta caaagactga actatgatga gtattttctc
     7801 cccctgtttg aacaattaac aagtctctta aatagtgcaa aagatcggtc acaaaacgag
     7861 tcatgctctt accattgtca aaaagaagat tcaagcaaga caaagctttg ggaacatcct
     7921 gttgagacaa ggccgccaca taatcatcca aggctgatag gctaatggtg ccagtaattt
     7981 cttcagagat agcagtcgtc agctcatttc cctgtgtcaa actcagggct tgatccaaaa
     8041 tagacaaggc gtcccgcatt ccaccttccg cccgtctggc aatgatttcc acagcctctg
     8101 gttcagaact gatattttct ttttctaaga tatagtgaat atgttcctta atatcctgtg
     8161 tcttaattga tttaaactca aaacgttgca cacgggatag aatagtagca ggaatcttgt
     8221 gcaattcagt agtggccaaa ataaagacta cattctgtgt tggttcttcc agcgtcttta
     8281 ggagggcatt aaaagcccct gtagacagca tgtgaacctc atctatgata taaaccttat
     8341 aacgagcaag gctaggcgca taggtagatt tatcacgaat ttcgcgaatt tcatctaccc
     8401 cattattaga agctgcatcc atttcaatga catcttctaa actaccgtcc gtcactgctt
     8461 gacaaatata gcagttattg caaggttcgc cacccacttg attgggacag ttcatagcct
     8521 tggcaaagat tttagcaaca ctggtttttc ccgttccacg aggaccagaa aaaagataag
     8581 cgtgacttat tttctcttgc tccaccgctt gtttaagagt cttagccaca acttcttgac
     8641 caactaactg ggagaagttt tgacttctat attttcgata aagtgcttga tacattaaga
     8701 tttttcctca aacatcgtaa agtcccatgt tgtcttttca agcaaaatag cgacaaattg
     8761 ttccaaataa tctcgatcca tagcatcgta atcctcaatc tctgaagaat ccagatccag
     8821 aactccaagt aactgaccat tcttcatcat cggcaccaca atttcacttt tagctagact
     8881 atcacaagaa atatagttga gataggtcgt cacatctcca acaataacag tttcctgaaa
     8941 gtgagctgcc tcaccacaaa cacccttgcc tagtgcaata cggatgcagg aaacacctcc
     9001 ttggaagggg cctaaaacca attcctttcc atcgaacaaa taaaagcctg caaatacggt
     9061 attaggaaaa cgtgatttta tgagagcact ggcgttggaa agattagcca aaacattggt
     9121 ttcgccttcc aataggaagg acaattcttc atttaacatt tgataacgtg attgtttttc
     9181 tgattttaac atagaactat tatatcaaaa aaggagcaga gacaaaagaa aaatcccttg
     9241 tttagtaaac
//
LOCUS       DAVLYR010000068         9127 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLYR010000068 DAVLYR010000000
VERSION     DAVLYR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.068"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(11..1516)
                     /gene="lytC"
                     /locus_tag="VYO63_001243"
     CDS             complement(11..1516)
                     /gene="lytC"
                     /locus_tag="VYO63_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1672164.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(1554..2312)
                     /locus_tag="VYO63_001244"
     CDS             complement(1554..2312)
                     /locus_tag="VYO63_001244"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1672165.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(2410..3087)
                     /locus_tag="VYO63_001245"
     CDS             complement(2410..3087)
                     /locus_tag="VYO63_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1672166.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLIKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(3096..4040)
                     /locus_tag="VYO63_001246"
     CDS             complement(3096..4040)
                     /locus_tag="VYO63_001246"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1672167.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(4222..4734)
                     /locus_tag="VYO63_001247"
     CDS             complement(4222..4734)
                     /locus_tag="VYO63_001247"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1672168.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(4821..5579)
                     /locus_tag="VYO63_001248"
     CDS             complement(4821..5579)
                     /locus_tag="VYO63_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1672169.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            5787..5933
                     /locus_tag="VYO63_001249"
     CDS             5787..5933
                     /locus_tag="VYO63_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672170.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(6080..7210)
                     /gene="ugpC"
                     /locus_tag="VYO63_001250"
     CDS             complement(6080..7210)
                     /gene="ugpC"
                     /locus_tag="VYO63_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1672171.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(7493..7578)
                     /locus_tag="VYO63_001251"
     tRNA            complement(7493..7578)
                     /locus_tag="VYO63_001251"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7542..7544),aa:Leu,seq:aag)
     gene            complement(7660..8235)
                     /locus_tag="VYO63_001252"
     CDS             complement(7660..8235)
                     /locus_tag="VYO63_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1672172.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFSPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(8235..9023)
                     /gene="codY"
                     /locus_tag="VYO63_001253"
     CDS             complement(8235..9023)
                     /gene="codY"
                     /locus_tag="VYO63_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1672173.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT         2692 a         2048 c         1533 g         2854 t
ORIGIN      
        1 aaatcatcgc ttaataccaa acgctgacat ctacgcgccc ttggattcct ttcatgtatt
       61 cagaagaggt atattgccaa cctttttctc ctgaataata agggttttcc cattctaaag
      121 cattcgtata ggccgctacc cagtttacat gttttaaaat atctgggtgt tttaaacgcg
      181 tctgtaataa actacgatag ctatagacat atacattttg ataacccgcc tgcttcatcg
      241 tgtccatgta cttgttgatg attttaaccc aagtgtctgt atcacttgga gctctcttgc
      301 tcttatttac atattcccag ttctcaacat cataatagat agggtaagac aggttcatat
      361 tgtatttctt tataagttca atggtctgtt tagcgtcatt ctcagcatcg gtctcatttt
      421 cagcataggt atagagatag acaccataag gaattcccag acggtttaac tccttaatgt
      481 tatgcgccaa ttccttgtct tctttaccgc tataacctag acgaacaatg acaccatcca
      541 cctcgttctc atcaataacc tttttccaat cattgatacg accattgtgc tcactaatat
      601 caatgatttt cttagcatgt tcggttccca cttgttcttc tctattatta aagaaatagc
      661 gtttaccatc tctgtgaacc cagccgacat tcaaatcttt cttttctttg agctcaccag
      721 aggccgcaaa gatatagcga gcaccatcca taatcacttc gtcagtcgcc atggcaccac
      781 ttccagtcaa atagtagttg ccttgccact cattccgagc catatagccc cacttcttga
      841 aatagtacca tttgccgccc actttttgcc actcttggtt agcataagtt ccatcggatt
      901 ttagataaaa ataagcagaa taggctggat catagagcca ttcattttgt atcatagctc
      961 cttgaccatt caagaaataa cttccttgcc attggctttt agccatgtaa ccccacttct
     1021 tgaagtagta ccacttattt ccaatcaatt gccattcttg atgagcataa gccccatctg
     1081 acttgagata aaaccaactc ttgtaattac tgtcatagat ccactcgttt tgggccatat
     1141 atccacctga tttcaggtag taatttccat gccattcatt ctgcgagtaa cgaccatctg
     1201 aattaatata aaaccaagcc ttatagtagt tgtcaaaaat ccattcactt tgagctttag
     1261 aaccatcatc ctttacataa taatccccat cccagtgggc agcagcatta gtctgtactt
     1321 ctgcgctcgt ttgcgtttta ttagaagact gattttgctc tgaactactt gaagctgtcg
     1381 ttgtatcctg cgaagttttt gctacttcag tttcatttgc agcgacatga ctagttgcca
     1441 agcctagtaa acagatactt gctaatccaa tttttatttt caatctttcc tctcctataa
     1501 aaaatgtaac agacatctga atgctgttcc acctagcttt tgctacttac tgattatttt
     1561 acaaagtcaa gcaaagccaa gaagctttca gcttcaagtg acgcaccacc tacaagggca
     1621 ccgtcaacgt ctgggcaagc catgtatgaa gcaacatttt caggtttaac agaaccaccg
     1681 tattgaacac gaactttgtc tgcgacttct tgaccaaagt cagcagctac aacgtcacga
     1741 acaactttac acattttttg tgcatcatct tgtgaagctg atttaccagt accgatagcc
     1801 cagattggct cataagcgat aactgaggca gcaacttgtt cagcagtcaa tccagccaat
     1861 gcagcagata cttgagcacc tacgaattca gcagctttac cagcttcgta agtttcaagt
     1921 gattcaccac aacagatgat tggaagcata ccgttcgcaa agattgcttt tgcttttttg
     1981 ttgatatctt catcagtttc atggaagtag tcacggcgtt ctgagtgacc gataacaacg
     2041 tagtcagtac cgatttcttt caaaacttgt gggctagttt caccagtgaa agcacctgca
     2101 ttttcaaagt agcagttttg agcagcaact ttaaggtttg agccttttgc aacagcaaga
     2161 acagttgtca aatcaagagc tggagcagcg atacctgctt caacaagatc tgatgaagga
     2221 agttttgatg caactgcttc aacgaatgct ttagcttctt ctggattttt gttcattttc
     2281 cagttaccag cgataaatgg tttacgtgac atttcacata cctctttttt caatttattt
     2341 tctatttatt ctatcacaat tatacacagc ttgcaaatct tactcggatt tcaagcctac
     2401 ttgcatggat taatccttcc agagatccat ggcatttctg aaatctgcag atacctgtgt
     2461 caactgagga ttgcttgctt ctctttctgc tctcttggcc tcaatttgag ccacactctt
     2521 gattccttca tggcgccagt ttctcaaaat cgcctgaatg tacttccagt ttggttttcc
     2581 attcaaaaca gcttcacgaa gagcctcctt aatcaagtca gcactggttc catcttcctt
     2641 tagtgtcttg atcaaatcct caatctcaaa aggcgtcaac aatcgtccca actcctgctg
     2701 gaaggtttcc accaaatcct tgagctggtt ttgaggtgtt agctggtctg aacttgaacg
     2761 aactgctcca aacaggtcat ccaaacgttc caaggccaaa ctagcatcaa agagcaattc
     2821 aatttcgcca tttaattcga tagtacgata ctggagcagt cccctttccg tcagattaga
     2881 aatggactgg ttgacatccg aaatttcctt gccaatcctt tcagcaatct ggcttggcga
     2941 catttcttct aagcctgtcg tattttgcaa atagaaaaat tgccaaacca gaaaatcgtc
     3001 gctagaagga aagagttcct taaaatgcaa gagcagggca ctcggtaaaa ccaaggtacc
     3061 tgatttaaaa gcgtctaaat atgtcataat tcctcttata aatacccata tgcagatgca
     3121 tcatcttcta tctttctcca atcaaaaggc gtctcctgat agaccgcatg gtttacccaa
     3181 ttactgaaaa agagggctgc agatgaagac caacaaagac aaggtacctg attaacatca
     3241 tcatctttaa agtaattttc tggaatatgt gggtcgaaac ctgcatcacg atctcgaaaa
     3301 tactcttttg ccaaggtatc acggtcatac tccaaatgac caaaactata aatttctcgt
     3361 aaatcacgac tggccaaaat agaaacccca acctgaggtc cttctgataa aatctcgaga
     3421 ttggtcttgt ttaagacctc ttccttagaa atctccgtgt gccgtgaatg aggggataca
     3481 tagctatcat caaagcctct aaatagaagg tgaccctctt ttagggtgtc ctgaggataa
     3541 atacctgata gcttactgtc catctggtat ttttctacac catagcgcag ataaagccca
     3601 gcctgagccc cccaacagat atgaagggtc gaatagacat gagtcttaga ccactcaagc
     3661 atctgtctaa attcctccca atagtccact tcctcaaatg gtaaatgctc aactggagca
     3721 cccgtgatga tcatcccatc aaaatactca tccttgactt caggaaaagt tttatagaag
     3781 gtctccatgt gctctgaacg agttgtttta gaacggtggc tctccatata gagaaaatca
     3841 atatccagtt gtaggggtgt attagccaag tggcgcaaca actgggtctc tgtgaccatt
     3901 ttctgtggca tgagatttaa aattaaaatc ttcaaaggac ggatatcttg gtgggcagca
     3961 cgttgatcat ccatgacaaa gatattctct gtccgtaaaa tctcaacagc tggcaatttt
     4021 ttatcaattc gaatcggcat aaccctctcc taactgtact ctttcaggaa taattgcata
     4081 tgtttgaaga caaatctcaa acacttagtc cttttattat actgcaagaa gatatagttt
     4141 tcaattatag tttttctcta actagttata gtctattttt atatcctagt gtagagaaaa
     4201 cagccctagg gactgttttc attaataatg cataagaact ttgtagtcgt agtcaccaat
     4261 tttttcacgg ccgttcaatt catccaattc aacaaggaag gcacaacctg ccataacacc
     4321 accaagtttt tcaatcatct cgatagttgc cttaacagtt ccacctgtcg ccaaaaggtc
     4381 atctacaata agaacacgtt gacctggctt aatggcatcc gcgtgcatag tcaaggtatc
     4441 gacaccgtac tctttttcat agtcagcaga aataacttcg cgtggcaatt tacctggctt
     4501 acgaacaggc gcaaaaccaa ttcccaactc aaaggcaact ggacaaccca cgataaatcc
     4561 acgagcttca ggtcccacga tcatgtcgac tttcttgtca gtagcatact gaacgatttc
     4621 acgaacagcg tagctataag catttccatc agccatcaaa ggactaatat cacggaaggt
     4681 aatgccttcc tttggataat tttcaattgt tgcaatgtaa tcttttaaat tcatcttttt
     4741 ctttctttca aagtttttta ctctctatta tagcatattt tttaagaaag aaaaaaggaa
     4801 aagttaactt caataattat ctaacgtttt gacgatttat aactagccat cgcaataaag
     4861 cccaatttct gtttattctt agcaaacatt ttatacatag ttaaaaactg ctttctattc
     4921 tcctttttac aagcatttac acaaattttc aaagttccta gcaaaccttc gtcataaatc
     4981 atacccgata atttcattaa tgtcatttca ccagtcaatg ctttcacatc acaataacct
     5041 gattctatca tcacctgttc ccaaccatct tgagttaaag gacctacatt tacatgaatt
     5101 gcttgtgata attcctgtct gatagactct ttagcttcct taagaagcac atcatgtgtc
     5161 aagagaagac ctccaggttt taataccctt agatattcca ttacacattt tttcttagct
     5221 tgatcggctt gcatagtcag catagcttca tttataacaa tatcaaaact agcatcttga
     5281 taaggaagtt tcattgcatt tgctctttca aaactgatta aatgagcaac acctgccgtt
     5341 ccagcagatt ttttagccac ttctaaagct tgagcatcca tatcaacagc agttatcttg
     5401 caaccaaaac gctgtgccaa ctcaattgct gtagttcccc tattacacgc aacctctagt
     5461 attctctttt cttttgaaaa tcctccttct gcaattaacc aatctgtggc acgctttcca
     5521 cctggacgta agcgtttttt ccccaatttt gctaaaaact tatgacctgc ttctgacatt
     5581 tgaacacctc tgttttttct tcattataac acaaaatgat attatctgac aattctaatc
     5641 tattatttga taaactagaa tcaaaagcat acaagtcacc ataactatag cataaattta
     5701 ttattacctc tctagttcga attcaattgc tcacgtagtt tacgcatata gcttcacttg
     5761 tagatggtac ccacaaataa tactcaatgg aaatcaaaga gcaaactagg aaactagccg
     5821 taagctgctc aaagtacatc tttgaggttg cagataaaac tgacgaaatc agtaatcata
     5881 cgtacggcaa ggcgacgttg acgtggtttg aagagatttt cgaagagtat taaagagcat
     5941 cacaatatag atgcataata tgacttccct agcaaaaagg agagaagcat tttgtttctc
     6001 tccctagatt aactgatatc tctccactac aattgataaa gagatatctt ttcttgtagt
     6061 gctttgaatt ttatttattt tagtagattg ttttttcagt ttctacatcg aagaagtgtg
     6121 ctttgttcaa gtcaaatcca agctcaactg ttgcacctgt ttgcaagtag tcacgagcat
     6181 caacttttgc aacaaactcg tctttaccaa cttggcagta aaggtgagat tctgaaccaa
     6241 gcagttctga tacagagata gtcgctttta caacacagtc tgggaatgtt tcaaggaaag
     6301 caggttctgc attcacgtct tctggacgga taccaaagat caattctttt ccttcgtagc
     6361 ctttttcacg aagaactttc aatgctcctt ctggcacttt caaacggaaa ccgtcagaaa
     6421 caatttcgct accaaccaat ttcacggtga tgaagttcat agctgggctt ccgatgaatc
     6481 ctgcaacgaa tttgttaact ggatttttgt aaacttcttg aggagtaccg atttgttcta
     6541 cacgtccgat agtacctgta ccagcagggt tcttagtagc tgacataata acgatacggt
     6601 ctgcaagtgt catcgcttct gtttggtcgt gagttacata gatagttgta gctccgatac
     6661 gacggtgaat tttagcgatt tcagcacgca ttgatacacg aagtttggca tccaagtttg
     6721 acaaaggttc gtccatcaag aatactttcg catcacggac aatcgcacgc cccatggcaa
     6781 cacgttgacg ttgaccacct gaaaggtcag ctggtttacg ttccaagaat tctttcaatc
     6841 caagtatttc agctgcttct tgaacacgtt tgttaatgtc ttctttgctg tatttacgca
     6901 atttcaaacc gaaagccatg ttgtcataaa cagtcatgtg tgggtaaaga gcgtagtttt
     6961 ggaataccat ggcgatatca cggtcttttg gagctacgtc gttgacaacc acgccatcga
     7021 tagatgcagt accttctgta atgtcttcaa gaccagcaat catacggagt gtagttgatt
     7081 taccacatcc tgaaggtcct acgaaaacga taaattcttt atctttgatg ttcaagttga
     7141 aatcttcaac tgaatagtgt tcgctgtttg gatatttttt gtaaatattt ttaagattca
     7201 attctaccat gaggtgaact ccttttgtct tttgataatt atattataga tgaaaacgct
     7261 ttatatttct atggcaagat gaccaaaaaa ataaaaaagt tctgtgcaac ttgcacaaaa
     7321 ctttagagaa tatcggagaa tttgaagaag ataatcttta ttattcttct atgacaaaag
     7381 cagaaacaag gatcagtttt cccatatttt cgctgattct ccactacatt tgactggatt
     7441 ctaatttttt agagaaatac aaaagagcta gctttagcta actcttttcc tatgcggaga
     7501 gagggacttg aaccctcacg acctaaagcg gtcacaggat ccttagtcct gcgcgtctgc
     7561 caattccgcc atccccgcat cgattacttt actagtatat caacttttgg gatgcttgtc
     7621 aacacttttt ttcaaatttt ttcattttca ccaaccaggt tactcagaaa gttcatttag
     7681 attttcatct actaacttag ctccgagtgt atttttgaaa tgacctaggg caaattgatg
     7741 attttcaggc cagatggaag caacagctgg tttaacaatc tcgatgtcat atcctagatt
     7801 ataggcatct atagctgtat gtaggacaca gatatccgtc aagacacctg ttaagataac
     7861 ggtagacact ctacgctctc tcaaacgaat atctaggtca gtccctgaaa aagctgagta
     7921 atggcgttta tccatccaaa agacacgact gtctgaacca tgctcttgat aaaagatccc
     7981 caaatctcca tataaattcc gtccactcgt cccaatcaga ttatgaggag aaaataactt
     8041 actttccgga tggaaacaat cgttttcttc atgagcatca atagtaaaga agatataatc
     8101 tcctcgttca aaagctaatc gagttacctt gctgatggca tccgaaatcg cctgagctgg
     8161 agcacctgct gttagtttcc cactatcagc aacaaaatct tctgtataat caatcgaaat
     8221 taaagccttt gtcattagta atctcttttc ttcacttctt caaaaatatc tgaaatcaag
     8281 accttaagat aggttccctt cattccaagt gagcgacttt caataatccc cgcagactca
     8341 agtttacgaa gagcattgac aatcacagag cgagtgattc cgatacgatc tgcaatcact
     8401 gacgcagtca acttcccttc atttccattt aattccccta aaattgctga aacagcacgg
     8461 agttcggagt aagaaagggt attgaccgcc atggtgacag cagtacgacg acgaatattt
     8521 ttctcatctt cttcacgttg gaagttaaga agctgaatcc caacaacggt actggcaatc
     8581 tcaacaagaa ccaagtcctc atcttcgaat tttttatcat tacgccaaat aatcaaagaa
     8641 ccaaggcgaa tccccgatac atgaatcggt gcaatagtcg tcaagccatc tggaaaatca
     8701 tctctactct caacagggaa aatactcata tcatgctcaa caggcaagtt tgcttctgtt
     8761 tcgtaaatca tattagcccc ttgaacgtag tcatctggga aaatcttagt ttggaagaat
     8821 tgctctacgc gatctgtatt tgttttataa cgcataaaat agccaagcag acgtccctta
     8881 ctattgataa tgcaggcatt gcaatgaata atatccgcta actgacgcgt aatagcgttg
     8941 taaggaagct catcctgcaa ctgctcctct gagcgtttca aaattgatgt aatttttcta
     9001 gttttttcta ataaatgtgc catttttcac ctcgaattta atcgctatca ttataacata
     9061 aaaacgtctc tttttcaata attatctgaa aattccttat tgacttgcat tgacttacaa
     9121 tttaatt
//
LOCUS       DAVLYR010000069         9123 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLYR010000069 DAVLYR010000000
VERSION     DAVLYR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.069"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            379..1854
                     /locus_tag="VYO63_001254"
     CDS             379..1854
                     /locus_tag="VYO63_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1672174.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1820..2535)
                     /locus_tag="VYO63_001255"
                     /pseudo
     CDS             complement(1820..2535)
                     /locus_tag="VYO63_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2790..3650
                     /gene="speE"
                     /locus_tag="VYO63_001256"
     CDS             2790..3650
                     /gene="speE"
                     /locus_tag="VYO63_001256"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1672175.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            3647..4906
                     /locus_tag="VYO63_001257"
     CDS             3647..4906
                     /locus_tag="VYO63_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1672176.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4906..6033
                     /gene="nspC"
                     /locus_tag="VYO63_001258"
     CDS             4906..6033
                     /gene="nspC"
                     /locus_tag="VYO63_001258"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1672177.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6030..7115
                     /gene="aguA"
                     /locus_tag="VYO63_001259"
     CDS             6030..7115
                     /gene="aguA"
                     /locus_tag="VYO63_001259"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1672178.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7125..8000
                     /gene="aguB"
                     /locus_tag="VYO63_001260"
     CDS             7125..8000
                     /gene="aguB"
                     /locus_tag="VYO63_001260"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1672179.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8181..8990)
                     /locus_tag="VYO63_001261"
     CDS             complement(8181..8990)
                     /locus_tag="VYO63_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1672180.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2653 a         1776 c         2061 g         2633 t
ORIGIN      
        1 gatgatgttt tctagcaaat ataaacccga gtaaaaaatg cctacggaca ggcagggttg
       61 aatgccgaag cgtggttgaa aagccacatt attgataggg ttaaaagcct acttttataa
      121 gttgatgtta ggacacttgt cctaattcat aaatttttag tgtggtgaaa gcacacgtca
      181 tcttgtgaaa cgatcaataa agtacgtaat atttgctact agagagttag gaaacatcgg
      241 gaacagacat actcaacaga aaccaaaata aacacgtcag aagattgcag agcaggtgaa
      301 aacctgctct tttttcatga gtcaaccttt agttccttag ttttcataag gtcctaaaaa
      361 tattgaaagg agtatgtttt gaaagagtta gatcaaaacc aagccccaat ttatgaagcc
      421 ttggtgaagt tacgcaagaa aaggattgtt ccctttgatg ttccaggtca caagcgtgga
      481 cggggaaatc cagaacttgt cgaactctta ggagaaaaat gtgtaggcat tgatgtcaat
      541 tcgatgaaac ccttggataa tttaggccat cctatttcga ttattcgtga tgcagaggag
      601 ctggctgcag atgcttttgg agctagccat gcctttctaa tgattggtgg aacaacttca
      661 tcggtgcaga ctatgattct ggcaacctgc aaggcaggag ataagattat tctgccacga
      721 aatgtccata aatctgctat caatgcgttg gttctatgtg gtgccattcc catctatatc
      781 gagatgagtg tagatcctaa gattggtatc gctttaggtc ttgaaaatga ccgagtagca
      841 caggccataa aggaccatcc agatgctaag gctatcctaa tcaacaatcc tacttactac
      901 ggcatctgtt cagacctaaa ggggttgaca gaaatggctc atgaagctgg catgatggtt
      961 ttagtagatg aagcccatgg agcgcatttg catttcactg gtaaacttcc aatttctgct
     1021 atggatgcag gagctgatat ggcagcagtt tccatgcata agtctggtgg gagtttgaca
     1081 caaagttcgc ttcttttaat cggggagcag atgaatcccg agtacgttcg tcagattata
     1141 aacctgaccc agtctacatc tgcctcttac ttattgatgg ctagtttgga tatttcacgt
     1201 cgcaacttgg cccttcgtgg taaagagtcg tttgaggaag tcattgagct atccgagtac
     1261 gctcgtcatg aaatcaatgc cattggcggt tactatgctt actcaaaaga gttaatagat
     1321 ggtgtgtcgg tctgtgattt tgatgtaacc aagttgtcag tttacactca gggaataggc
     1381 ttaacaggta tcgaggttta tgacctcttg cgagacgaat acgacattca gatcgagttt
     1441 ggtgatatcg gcaatatctt ggcctatatt tccatcggcg accgcatcca agacatcgag
     1501 cgcttggttg gtgctctggc tgatattaag agactctatt cacgagatgg gaaggactta
     1561 atagctggag aatatattca gcccgagttg gtgctgtctc cacaagaagc cttctattca
     1621 gagagaaaaa gtttgacctt agatgagtct gttggacagg tctgtggaga atttgtcatg
     1681 tgctatcctc cagggattcc tatcttggct cctggtgaac gcattacacg agaaattgta
     1741 gactatatcc aattcgccaa ggaacgtggt tgctccctcc aagggacgga agatccagag
     1801 gtcaatcata tcaacgttat taagagaaag acaaactata agaaaagtca atagttttat
     1861 ctaaactatt tcttatttca atttgatgat ttggcgatga ttttagagca cggcaaaaag
     1921 cccttgaatt agaagcggtc aatcgcttaa tttctatcag cttatcaaat cctgcctcaa
     1981 tccttttctg aggattaggg tagcgtgtca agagttggta ggtatattct gaatgctttc
     2041 caacgatttt atccaactca ggaaagatga tatcaagaca acgagtgtat tgtactttcc
     2101 aatcagactg tttttcttga gacgatgaat atgtctagcc agtattttta gttctacttg
     2161 ccgattatcg tgttgaaatt gttcacgatt ggggtcagaa agaagtttaa gagcgatgcc
     2221 atgagcgtct ttcttatccg ttttagtctt gcgaagtgat aatgatttgg caaattcctt
     2281 gatgagcaaa ggattgtagg tgtaaacttt atatccttgt tcatgcagga agttcagtag
     2341 attaaaggca taatgtccag tatcttcaag agcgatgaga cagtcttggt tgagctgtcg
     2401 aagagacaga tctaagagtt caaaaccagc tttattattt gaaaaagtga gtggtttaag
     2461 aacagttttt ccttgaacat tcaaggctgt aacatcgtgt ttatttttag cgatatcaat
     2521 gcccacataa agcatgggag tacctccaga tgtagtattt caagtctact tggttatcca
     2581 cgaacttttt gccttgttac cttagacgag ataaaacgtc tatgcgttat caaactcatt
     2641 accaattgaa acaaaagctg tggttagagc ctttcggaaa tcgtcaagcg attggaggaa
     2701 atgaactaat ccacagtggc ttattccaag tataccactt gggctttggc agtagctaac
     2761 tgcgctaaat ataatataag gagaaataga tggatttatg gttttctgaa gttcatactc
     2821 cagatgtcaa attgtctctg agaacagcca agcaacttta cgctggaaaa agtgaatggc
     2881 aggatatcga agtcttggat acgccagctt ttgggaaaat actgatttta aatggccatg
     2941 tcttgttctc agatgcggat gatttcgtct acaatgaaat gaccgttcac gttcccatgg
     3001 ctgtccaccc aaatccaaag aaagtattgg ttattggggg tggtgacggc ggtgttgccc
     3061 aagtattaac cctctatcct gaactggagc aaattgatat tgtggaaccg gatgagatgt
     3121 tggtcgaggt ctgtcgtgag tatttcccag actttgctgc agggctagat gatcctcgtg
     3181 ttaccattta ctaccaaaat gggctacgct ttttgcgaaa ctgcgaagat gattacgata
     3241 ttatcatcaa cgatgcgaca gatccatttg gccatacgga aggactcttt accaaggaat
     3301 tctacggcaa tagttatcga gctctgaagg aagacggcat catgatttac cagcatggga
     3361 gtcccttctt tgacgaggat gagtcggcct gccgaagcat gcaccgcaag gtcaatcaag
     3421 cctttccaat cagtcgggtt tatcaggccc atattccaac tagcccagct ggctattggt
     3481 tgtttggatt tgcatcgaaa aaataccacc ctgtcaaaga ttttgacaag gaaggctgga
     3541 aaaaacgcca gcttttcaca gaatactaca ctgcaaactt acacgtggga gcctttatgt
     3601 tgcccaagta tgttgaggac attttagaag aagaggaagg aaaaaaatga gtcgtttact
     3661 agttattggt tgtgggggcg ttgcccaagt tgctatttca aagatttgtc aagatagcga
     3721 aacatttaca gagattatga ttgctagccg taccaagtca aaatgcgatg acttgaaagc
     3781 gaagctagaa ggcaaaacaa gtactaaaat tgaaactgca gcacttgatg ctgacaaggt
     3841 tgaagaagtg attgccctga ttgaaagcta caaaccagaa gctgttttga atgtagctct
     3901 gccttatcaa gatttaacca ttatggatgc ttgtttggca acaggtgttc actatatcga
     3961 tacagccaac tacgaagcag aagacacaga agaccctgag tggcgtgcta tctacgaaaa
     4021 acgttgtaag gaacttggtt ttacagccta ctttgactac tcatggcagt gggcttatca
     4081 agagaaattc aaagaagcag gcttgactgc tcttcttggt tctggttttg acccaggtgt
     4141 aactagtgtc ttttcagctt atgccctcaa acactatttt gatgaaatcc attatatcga
     4201 cattttagac tgtaatggcg gtgaccacgg ttatccattt gcaaccaact ttaatccaga
     4261 aattaatctc cgtgaggttt ctgcgccagg ttcttactgg gaagatggga aatgggtcga
     4321 agtcgaagct atgtctatca agcgtgagta tgatttccct caagttggac aaaaagatat
     4381 gtatctcctt caccatgaag aaatcgaatc attggccaag aacattccag gtgtcaaacg
     4441 cattcgtttc tttatgactt ttggtcaatc ttacttgacg cacatgaaat gtcttgaaaa
     4501 tgttggactc cttcgtacgg ataccattaa ctttaacggc caagaaattg ttccaattca
     4561 atttttgaaa gccttgcttc cagatcctgc cagtcttggg ccacgtacag tcggaaaaac
     4621 caatattgga tgtatcttta caggtgtcaa agacggtgtc aaaaagacta tctatatcta
     4681 caatgtctgc gaccatcagg aatgttacgc agaggttggt tcgcaagcta tttcttatac
     4741 gacaggagtt ccagccatga ttgggacaaa attagtcatg aacggaactt ggaaacaagc
     4801 tggagtgtat aaccttgagg agttagatcc agatccattc atggaagctt tgaatgagta
     4861 tggtttgcca tgggttgtgg ttgaaaatcc acaaatggtg gactaatgaa gttagaacaa
     4921 gtaccaacac cagcctatgt tattgacttg gccaagttag aagctaattg ccgcattcta
     4981 caatatgtac aagaagaggc cggttgcaag gtcttgcttg cccagaaggc atattccctc
     5041 tacaaaactt atcccttgat tagccagtat ctatcaggta cgacagctag tggactctat
     5101 gaggccaaat tggcaaggga agaatttcct ggtgaagtcc atgtatttgc gcctgctttc
     5161 aaggatgcag acttggagga attgctagag ataatggacc atatcgtctt taactcagag
     5221 agacagttgc gtaaacacgg tccgcgttgt cgagaggctg gtgtcagtgt tggtttgcgc
     5281 ctcaaccctc agtgttcaac tcaaggagat cacgcgctct atgacccttg tgcaccaggt
     5341 tctcgctttg gagttactat agacaagatt ccgagtgatt tgctagattt ggttgacgga
     5401 cttcattttc ataccctttg cgagcaggga gcagatgatt tacaaacaac tttgaaagca
     5461 gtagaagaac agtttggtcc ctacttacat gaggtaaaat ggctcaatat gggtggtggt
     5521 catcatatta caagagaagg ttacgatgtg gatttgctga tttcagaaat caagcgtatc
     5581 cgaaaaactt acaatcttga aatctatatc gagcctggtg aagccattgc gcttaatgcg
     5641 ggttatttag caactgaggt attagatatt gtagaaaacg gtatggaaat cttggtttta
     5701 gacgcctctg cgacctgcca tatgcctgat gtacttgaga tgccctatcg tccacctttg
     5761 agaaatggct ttgagtcaca ggaaaaagcc catacctaca gactttcttc taatacctgt
     5821 ctgacgggcg atgtgattgg tgattatagt tttgaaaatc cagtccaaat cggagacaga
     5881 ctttattttc aagacatggc catttattct tttgtcaaaa ataatacctt taatggtatt
     5941 ggattgccaa gtctctatct catggacgaa cagggagact gtagcttact caaagctttt
     6001 ggctatcaag actttaaagg gagattatca tgatggacag tccaaaaaaa ttaggctatc
     6061 acatgccagc agagtacgaa ccccatcatg gtaccctcat gatatggccg actcgaccag
     6121 gatcatggcc ttttcaagga aaggctgcta aaagagcatt tactcagatc atcgagacca
     6181 tagcagaagg ggaaagagtc tatcttttgg tggagcaggc ctatctatct gaagcccaat
     6241 cctatcttgg agacaaggtt gtttatttag acattcccac caatgatgcc tgggcgcgtg
     6301 atactggccc aaccattctc gtcaatgata aaggtaagaa attagccgtg gattgggcct
     6361 ttaatgcttg gggaggcacc tatgatggtc tttatcaaga ttatgaagag gatgaccaag
     6421 tagccagtcg ttttgctgag gccttggaaa ggcctgtcta tgatgctaaa ccttttgtac
     6481 tggaaggagg cgcaatccat agcgatggtc aaggaactat tctcgtaact gaaagttgct
     6541 tgcttagtcc tggtcgcaat cctaacctga ctaaagagga gattgaaaac acattattag
     6601 aaagtcttgg tgctgaaaaa gttatttggc ttccttatgg tatttatcag gatgaaacca
     6661 atgaacacgt cgataatgtt gctgcctttg ttggtcctgc tgagcttgtt ttggcttgga
     6721 cagatgacga aaatgatccc cagtatgcca tgtcaaaagc agatctcgaa ctcttagaac
     6781 aggaaacaga tgcaaaaggt tgtcacttca ccattcataa attgcctatc cctgcagttc
     6841 gacaagttgt gacagaagaa gatttgccag gctacatcta tgaagaagga gaagaaaagc
     6901 gatacgcagg tgaacgacta gcagcttcct acgtaaactt ttatatcgcc aacaaggctg
     6961 tcttggttcc acagtttgag gatgtaaacg accaagtggc cttagatatc ctcagcaagt
     7021 gtttcccaga ccgtaaagtt gtcggaatac cagccagaga tattctctta ggtggtggca
     7081 atatccactg tatcacccaa caaattccag aataggagaa aaagatgaga aatgtaagag
     7141 ttgcaaccat tcagatgcaa tgcgctaagg atgtggcaac aaatatccaa accgcagagc
     7201 gtttagtacg tcaggctgct gagcaaggag cccaaattat tctcttgccc gagttgtttg
     7261 aacatcccta tttctgtcag gaacgtcagt atgactacta ccagtatgcc caatctgtag
     7321 cggaaaatac tgccattcag cattttaagg tgattgctaa ggaactacaa gttgttttac
     7381 caatcagttt ctatgaaaaa gatggtaatg tcttgtataa ctctattgcc gtcattgatg
     7441 cagatgggga agtgctgggc gtttatcgaa agacccacat accagatgac cattattatc
     7501 aagaaaaatt ctatttcacg cctggtaaca ctggtttcaa ggtctggaat actcgctatg
     7561 ctaagattgg tatcggtatc tgttgggatc aatggttccc tgaaacagcg cgctgtcttg
     7621 cattgaatgg tgctgaattg ctcttttatc caacagccat cggttcagag ccgattttgg
     7681 atacagatag ttgtggtcac tggcaacgta ctatgcaagg gcacgcagca gcgaatattg
     7741 ttccagtcat cgcagccaat cgttatggtt tagaggaggt tactcctagt gaggaaaatg
     7801 gcggacagag ttccagtctt gacttctacg gttcctcctt tatgacggat gaaacaggag
     7861 ctattctaga acgagctgaa agacaagaag aagctgttct gttagctact tataacctag
     7921 acaagggagc aagtgaacgc ctaaactggg gcttgtttcg agatagaaga ccagaaatgt
     7981 atagacaaat tacagattag tgtgggagaa atgagagatt cattctgcta gactaacttc
     8041 ttattagcaa ctataagata ctatggcatc tagtaaatcg atttttatga ttcgctattc
     8101 ttctctattg attagtccgt attttaaaat attagcaaaa aagcaaatag cagtaacttc
     8161 tgtctatttg cttttctttt ttatagaata tatttctcaa tagcacgcgc aacgccgtct
     8221 tcttcgttgc ttgaggtaac ggcatccgca agagatttga cataatcgct ggcatttccc
     8281 attgcaatcc caagccctgc aaactggagc atttcgatat cgttattagc atcgcccatg
     8341 gccataatct ctgaggaatc aatcttcaaa atctcagcta gtcgtgaaag agcagtagcc
     8401 tttgtcgttc caagtggcat tgcttcataa atgacaggct gcgaacgaac tccactgaat
     8461 cgttggcaaa gctcttcagc aaaacgctgc tcaaaatcgt ctgtttgttc ttttgttcct
     8521 aaaaacatac cttggaacat ccggaacttt ccactagtcg cttcctcaag agaaatttca
     8581 gtcaggtctg aaaatactag tttagcatca ttttcaataa cttgattggg ctttccaccg
     8641 agaacaaaat aatgtgactc gtcaaaaagt gtcaactgaa catcactctt ttcagcaagg
     8701 tcatagaggt attcgatgtc agctggacta agttcttgcc agtcaaccaa gccccagtcg
     8761 ctagtctggt gagttgaaca accgttgtta acaatcacat actcattctg gaggtcgagt
     8821 cccagttttt tgtagtaggg gaggacaccg aaaagggggc gacccgtaca gagaaccagt
     8881 ttgacacctt tttcaatggc tttgtgaata gcagtaatgt gagcttgtgg gatttccttg
     8941 gcttcattga ggagggtgcc gtccatatcc aaggctagta gtttaatcat aggtcttcct
     9001 tctttatctt ttgctattat tatagcatat tttggagaag aaattgatag aaagcttgag
     9061 actaattgat tttatagttt aagatgtttt gatgacaatt catgatttga agaggatatt
     9121 tcg
//
LOCUS       DAVLYR010000070         9091 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLYR010000070 DAVLYR010000000
VERSION     DAVLYR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.070"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            310..2097
                     /locus_tag="VYO63_001262"
     CDS             310..2097
                     /locus_tag="VYO63_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1672181.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2094..2570
                     /locus_tag="VYO63_001263"
     CDS             2094..2570
                     /locus_tag="VYO63_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1672182.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2598..3503
                     /locus_tag="VYO63_001264"
     CDS             2598..3503
                     /locus_tag="VYO63_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1672183.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3490..4305
                     /locus_tag="VYO63_001265"
     CDS             3490..4305
                     /locus_tag="VYO63_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1672184.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4312..4716
                     /locus_tag="VYO63_001266"
     CDS             4312..4716
                     /locus_tag="VYO63_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1672185.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4969..6135
                     /locus_tag="VYO63_001267"
     CDS             4969..6135
                     /locus_tag="VYO63_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1672186.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6251..7288
                     /locus_tag="VYO63_001268"
     CDS             6251..7288
                     /locus_tag="VYO63_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1672187.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7496..8350
                     /locus_tag="VYO63_001269"
     CDS             7496..8350
                     /locus_tag="VYO63_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1672188.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWNIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8500..8757
                     /locus_tag="VYO63_001270"
     CDS             8500..8757
                     /locus_tag="VYO63_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1672189.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8865..>9091)
                     /locus_tag="VYO63_001271"
     CDS             complement(8865..>9091)
                     /locus_tag="VYO63_001271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672190.1"
                     /translation="TDEVSSKHCFEVVDRTDEVSNHIYGKVKLTWFEEIFEEYKSPHN
                     KTHTIKVFMYCPPKVRQKESNFWGAVHFNT"
BASE COUNT         2611 a         1700 c         2049 g         2731 t
ORIGIN      
        1 ccataaaagc gaaaaaaata gtagtctttt gaataaaaaa ttagaataat agtttgaaaa
       61 attaagcgat atagggattg ctcatatttt ataaatgtat tctatacaaa attattttag
      121 gtcctatatt ccgaatttaa agagcgtttg aaatgggtct aaaaatgttt gaaactaatt
      181 gaatgtgtgt ataatagtaa aaatgcagta aatatcccga caatttctat tgacaattca
      241 aacagattgg tttataatta atataacaac aaatgaaagc gcaaactttc gcggtcggaa
      301 ggtagtttta tgacacgatt tgagatacga gatgatttct atctcgatgg aaaatcattt
      361 aagattttat ctggtgccat tcattatttt agggttcctc cagaggattg gtatcattcg
      421 ctctataact tgaaggctct tggttttaat acggtagaga cttatgttgc ttggaattta
      481 cacgagcctc gtgaaggtga gtttcatttt gacggtgatc tggatttaga gaaatttctc
      541 caaatagcgc aggatttggg tctctacgca attgtgcgtc cgtctccatt tatctgtgcg
      601 gaatgggaat tcggtggctt accagcttgg ctcttgacca agaacatgcg aattcgctca
      661 tccgacccag catatatcga ggcagttggt cgctactatg atcagttatt gccaagactg
      721 gttcctcgtt tgttggacaa tggtggcaat attctcatga tgcaggttga aaatgagtat
      781 ggttcttacg gagaagataa ggcttacctg agagcgattc gacagctaat ggaagagtgt
      841 ggcgtaacct gtcccctctt tacatcagat ggtccatggc gagctactct gaaagctgga
      901 accttaattg aagaggacct ctttgtaaca ggaaactttg gttctaaggc accttacaac
      961 ttttcgcaga tgcaggaatt ctttgatgaa catggtaaga aatggccact catgtgtatg
     1021 gagttctggg atggttggtt taatcgttgg aaagaaccga ttatcacacg ggatcctaag
     1081 gaattggcag atgcagttcg agaagttttg gaacaaggct ccatcaatct ttacatgttc
     1141 cacggtggta caaactttgg tttcatgaat ggttgctcgg ctcgaggaac tttggacctg
     1201 ccacaagtta cgtcttatga ttacgatgcc cttctggatg aagaaggaaa tccaactgct
     1261 aaatatcttg cagtcaagaa gatgatggca acacattttt cagagtatcc gcagttggaa
     1321 ccactctaca aagagagtat ggagttggat gctattccac tagttgaaaa agtttctttg
     1381 tttgaaacct tagatagctt gtcaagtcct gtagaaagtc tctatcctca aaagatggag
     1441 gagctgggac aaagttatgg ctacctactt tatcgaacag aaacaaactg ggatgcagaa
     1501 gaagaaagac ttcgtatcat tgatggtcga gatagggccc agctgtatgt cgatggtcag
     1561 tgggttaaaa ctcaatatca gacagagatt ggggaagata ttttttatca aggtaaaaag
     1621 aaagggctat ctaggttaga tatcttgata gaaaatatgg ggcgtgtcaa ctatgggcat
     1681 aagttcttag cggatacgca acgtaaggga attcggacag gggtctgtaa ggatctgcat
     1741 ttcttactaa actggaaaca ctatccactc ccactagaca atcctgagaa aattgatttt
     1801 tcaaaaggat ggactcaagg acaaccagcc ttttacgctt atgactttac agtcgaagag
     1861 ccaaaagata cttacctaga cttgtctgag tttggtaagg gggttgcctt tgtcaatggg
     1921 cagaatctag gacgtttttg gaacgttggc ccaactctct cactttatat ccctcatagc
     1981 tatctcaagg aaggtgccaa ccgcatcatt atctttgaaa cagaaggtca atataaagaa
     2041 gagattcatt taactcgtaa acctacacta aaacatataa agggggaaaa cttatgacaa
     2101 ttgtaggatg ccgtattgat ggacgtttga tccacggaca agtagccaat ctttgggctg
     2161 gaaaactaaa tgtttcacgc attatggttg tagacgacga agttgtcaac aacgatattg
     2221 aaaagagtgg tttgaaactt gcgacaccac caggtgtgaa attgagtatt ttgccagttg
     2281 agaaagctgc agccaatatt cttgctggca aatacgatag ccaacgtctc tttatcgtgg
     2341 ctcgtaaacc agaccgcttc cttggtttgg tagaagcagg tgtaccactt gaaaccctta
     2401 atgttgggaa tatgtctcaa acaccagaaa ctcgttctat tacacgttct atcaacgtag
     2461 tagacaagga tgtggaagac ttccacaaac tggcagaaaa aggtgttaaa cttactgctc
     2521 agatggttcc aaatgatcca atttcagact ttttgagctt attaaaatag gaaaaaattt
     2581 tttaggaggt cattgttatg atacaatggt ggcaaatttt acttctcact ttgtactcag
     2641 cttatcaaat ctgtgatgag ttgacgatcg tttcatctgc aggttcccct gtatttgctg
     2701 gtttcattac tggtttaatc atgggagatg tgactactgg tttacttatc ggtggtaact
     2761 tgcaactgtt cgttcttggg gttggtacct tcggtggtgc ttctcgtatc gacgcaactt
     2821 ctggtgcggt tcttgcgaca gccttctctg tttcacaagg aattgatgca ccgcttgcaa
     2881 ttactacaat cgctgtacca gtagcagctc tcttgactta cttcgacgtt cttggtcgta
     2941 tgactactac cttcttcgct caccgtgtgg atgctgcaat cgaacgcttt gactataaag
     3001 gtattgaacg caactacttg cttggtgcga ttccgtgggc tctatctcgt gcccttccag
     3061 tcttctttgc ccttgctttt ggtggtgcct ttgtacaatc agtagtagac ttcgttgaag
     3121 cctacaaatg ggttgcagat ggcttgacac tcgcaggacg tatgcttcca ggtcttggat
     3181 ttgcaatctt gcttcgttac cttccagtta aacgtaacct tcactacctt gctatgggat
     3241 ttggtttgac agctatgttg actgttcttt actcatatgt aacaggtctt ggtggcgctg
     3301 ttgctggtat cgtaggtact cttcctgctg aagttgctga aaaaattggt ttcgtgaaca
     3361 acttcaaagg tttgtctatg attggtattt ctatcgtagg tattttcctt gcagtgcttc
     3421 acttcaaaaa tagccaaaaa gtagctgtag cagcaccttc tacaccatca gaaagtgggg
     3481 aaatcgaaga tgacgaattc taattacaaa cttacaaaag aagattttaa tcaaatcaac
     3541 aaacgtagct tgtttacttt ccaattaggt tggaactacg aacgtatgca agcttctggt
     3601 tacctttaca tgatcttgcc tcagttgcgt aaaatgtatg gtgatggaac tcctgaattg
     3661 aaagaaatga tgaaagttca tactcaattc ttcaatactt caccattctt ccataccatt
     3721 atcgctggtt ttgaccttgc catggaagaa aaagatggtg taggttcaaa agacgctgtt
     3781 aacggtatca agacaggttt gatgggacca ttcgctcctc ttggggatac aatctttggt
     3841 tcacttgtac ctgctatcat ggggtcagtc gcagcaacta tggctatcgc tggccaacct
     3901 tgggggatct tcctttggat tgcagttgca gtagcgtatg acatcttccg ttggaaacag
     3961 ttggaatttg cttacaaaga aggggttaac cttatcaaca acatgcaaag taccttgaca
     4021 gctttgattg acgctgcatc tgtacttggt gtcttcatga tgggtgctct tgtagcaaca
     4081 gtgattaact ttgaaatttc ttacaagttg ccaatcggtg aaaagatgat tgatttccaa
     4141 gacatcttga accaaatctt cccacgtttg cttccagcaa tctttactgc ctttatcttc
     4201 tggttgcttg gtaaaaaagg tatgaactct actaaagcta tcggtattat tatcgtactt
     4261 gctttggctc tttctgccct tggtcacttt gcacttggaa tgtaattcct tatgactaaa
     4321 tcattaattt tggtgagcca tggtcgcttc tgtgaggagc ttagaggtag cacagaaatg
     4381 attatgggcc cacaagacaa catttacaca gtagctcttc ttccagaaga tggcccagaa
     4441 gaatttactg ctaaatttga agctgttatt gaaggattgg atgatttcct agtctttgcg
     4501 gatcttctcg gtgggacacc ttgtaatgtg gtgagtcgct tgatcatgga aggtcgtgat
     4561 attgaccttt acgcagggat gaatcttcca atggtgattg aatttatcaa tgcgagcctt
     4621 acaggcgcag atgcggacta caagagccgt gctgcagaaa gcattgtgaa agttaatgac
     4681 ctgttagcgg gcttcgatga tgacgaagat gaataatact cttcgaaaat ctcttcaaac
     4741 tacgtcaacg tcgccttgcc gtaggtatat gttactgact tcgtcagtct tatccggcaa
     4801 cctcaaagcg gtgctttgag cagcctgcgg ctagtttcct acagatttta gttggaactc
     4861 gattcaattc atgtgacaac gtgaaaatcg ttagagcatt ttatatagaa tatacatggg
     4921 aatggagctt actcccattc ccatatttaa tagaaaaaga ggaactcaat gctacattat
     4981 acaaaagaag acttgctcga attgggtgca gaaatcacta cgcgtgaaat ctaccaacag
     5041 cctgatgtat ggagagaagc ttttgaattt tatcaagcaa aacgtgaaga aattgcagcc
     5101 tttctacaag aaatcgctga taaacatgac tatattaagg ttatcttgac aggtgctggg
     5161 acttctgctt atgtgggaga taccttgcta ccttacttta aggaagtcta tgacgaacgc
     5221 aaatggaatt tcaatgctat tgcgacaaca gatatcgttg ccaaaccagc aacctatttg
     5281 aaaaaagatg tggcaactgt ccttgtgtct tttgctcgta gtgggaactc gcctgaaagt
     5341 gtggcgactg ttgatttggc caaatccttg gtggatgagc tttatcaagt gacgattact
     5401 tgtgcaacag atggtaaatt ggctcttcaa gctcacggtg atgaccgtaa tctcttgctc
     5461 ttgcaaccag ctgtctctaa tgatgctgga tttgccatga cttctagctt tacgtctatg
     5521 atgttgacag ctctcttggt ctttgatcct acagaatttg ctgttaagtc tgaacgtttt
     5581 gaagttgtat ctagtcttgc ccgtaaagtt ttagacaagg tagaagatgt caaagagctc
     5641 gttgatttag actttaaccg tgtcatctat ctaggcgctg gtcctttctt tggacttgct
     5701 catgaagctc agctcaagat tttggaatta actgctggtc aagttgcgac catgtatgaa
     5761 agcccagttg gcttccgtca cggtccaaaa tctcttatca acgacaatac agttgttttg
     5821 gtctttggta caacgacagc ctacactcgt aagtacgact tggacttggt tcgtgaagtt
     5881 gctggtgacc agattgctcg tcgtgttgtg cttttgagtg atcaagcttt tggtcttgaa
     5941 aatgtcaaag aagtggtcct tggttgtggc ggtgtcttga atgatattta ccgtgtcttc
     6001 ccttacatcg tttatgccca actctttgct ctattgactt cactcaaggt agaaaataaa
     6061 ccagatacac cgtctcctac aggtacagta aaccgtgtag tacaaggtgt cataattcac
     6121 gaatatcaaa agtaagacag tgtttatgaa ttcttgacaa gaggatttgt aaattatcag
     6181 ataaaccata gattgtcagt acgctttcta tggtttgttt gcttgagaga aatagtaaaa
     6241 ggagaacaga atgaaagcat acacagagcg tgtatttgga aatgttgagg gcgaggatgt
     6301 cttggcctat cgatttgaga cagacggtgg ctaccaactt gaggttatga cttatggcgc
     6361 gaccatcttg cgctatgtcg cacctgacaa ggctggaaat tttgccaatg ttatcttggg
     6421 atttgatgac tttgatagtt atgtaggcaa tagtcccaag catggagcaa gtgtaggtcc
     6481 tgtagcgggt cgtattgcag gtgcgacctt tgagctcaat ggtaagacct atgaccttga
     6541 ggttaataat gctagcaact gtaatcacag tggttcaact ggttgggatt ccagcttgtt
     6601 tgaagttgaa gaagtaagcg atcatggctt gactctctac acagagcgta cagatgggac
     6661 aggagggttc cctggaaatc tcaagatttg gatcagttat cacttggaag aaactggtgc
     6721 ctatgaaatc atctacaagg taacgaccga tcaggatacg ctggtcaatc caaccaacca
     6781 cagctatttc aacttgtctg gtgatttcac gcagacgatt gaccgtcatg tcttccaact
     6841 aaacacagag ggcatttact caatcgctcc tgacggtgtt cctgccaaaa ctccagaagc
     6901 caaccgtgat gtggtcaaac acatctacaa tggtaccttg ttgaaggata tctttgcaga
     6961 agaagatgag caaatccagc tggcatcagg tttggatcac ccatttgccc ttcctgcagg
     7021 ccatgacaat gctggattcc tttatgacca aaattcaggt cgcttcctgc ttttcaagac
     7081 agaagctcct tgctttgtgg tctacacagc aaactttgtg gatgaaagtg tcatcatagg
     7141 aggtcagcca atgctacagc acaatgggat tgctcttgaa gcgcaagctt taccagatgc
     7201 cattcacagt gaccttaaag gccaagtcat tcttaaagct ggtcaaacct tcaccagtaa
     7261 gacacgttat gaacttgttg tgaaataaaa aaagctggtc aaaaagtctt taaaattaaa
     7321 ataacgcata ttgtcaggtg atgaaaagtt tgataatatg cgttttatta tgaaatgatt
     7381 tactttatct tatcctgaaa tggtgttatt tttgagttat ctggcttgac tttcttgatg
     7441 gaaattatat aatagttgta ataacaataa ttacaacgag aaaagaggac catttatgac
     7501 ctatgaatac aagagtcaca tttatttggc agagacagtt ttaaatgtaa aggatttggc
     7561 cagtcaaaca gccttttatc agcaagttat tggtttagaa atcttatctc aaactgagac
     7621 agagtccatt ctaggccttg gtggaaaagt cttggtacag ctgattcaag cacaagagag
     7681 cggagaagtg agggaacatt atggtcttta ccatttggcc attctcttgc ccacacgcaa
     7741 ggctttggct gatgtcttga aacacctgac agatttgcaa attcctcttg tcggcggtgc
     7801 agaccatggt tacagtgaag ctatttactt ggaagatttg gagggaaatg gcattgaact
     7861 ctatcgagat aagccagttt ccacatggaa tattcgagaa gatggacgca ttatcggagt
     7921 gacggaggct cttgcggcgc aggacattta tgagttgggg gaaagagtag agccctttat
     7981 cctggcagaa ggtacaagaa tggggcatat tcatctttcc gtcaaggata gtcgaaagtc
     8041 cagccagttt tatcaaaagg tgttagggct agagaataaa ttcagtgtgc ctggcgctag
     8101 ttggattgcg gctggagatt accatcatca tttagcagtt aacgaatggg gaggaaaagg
     8161 tctggcttcg cgtaagcaag gcttaccagg tttagcctac tatgtcatcg aagtcgcaca
     8221 taaaaaagaa ctgttaacga ttgcccaacg agcacaagaa gttgacgcac caatcaaatg
     8281 gatgacatcg agccaattgg aaatcacaga ctcagatggc atcgtgaccc gtattcgttt
     8341 agcaaggtaa aattaggaat ttttaataag ttataagtat tgctagtttg ctttttgaac
     8401 ggtctttggt aacagaactg cgtgagctgg atgggtgaga attttgacaa agaagctgat
     8461 tagagatata atgaagaaaa atcgagcaag gaaaaagaaa tgacaaattc aaagtatatt
     8521 acttgtttga aacgttcaga gggccagttg tgtggaattc aaaagatgat tgaaggagat
     8581 cgtgactgtg ctgatattgt gacgcaactg acagcagtga aatctagtgt agagcgcgtg
     8641 attgagatga taattaccga aaatcttact gaatgtatca atcagccact agatgattct
     8701 gaagctcaaa aggaacgcct agaaaaggct atccgatact tgattaaacg gaaataaatg
     8761 aatgttttga gacgatatgt gcaaaggtct attaactatt agaatagttt aatgtgagtc
     8821 aaaagaagtt gtaaaaaatc tttaaaatta aaaaacgcat agtatcaggt attgaaatgt
     8881 actgcccccc aaaagttaga ttctttctgt ctaacttttg gggggcagta cataaaaacc
     8941 ttgatagtat gcgttttatt gtggggagac ttatactctt cgaaaatctc ttcaaaccac
     9001 gtcagtttca ccttgccgta gatatggtta ctgacttcgt cagttctatc cacaacctca
     9061 aaacagtgtt ttgagctgac ttcgtcagtt c
//
LOCUS       DAVLYR010000071         9023 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLYR010000071 DAVLYR010000000
VERSION     DAVLYR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.071"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..254)
                     /locus_tag="VYO63_001272"
     CDS             complement(<1..254)
                     /locus_tag="VYO63_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1672191.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEK"
     gene            complement(305..469)
                     /locus_tag="VYO63_001273"
     CDS             complement(305..469)
                     /locus_tag="VYO63_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1672192.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            complement(550..3201)
                     /locus_tag="VYO63_001274"
     CDS             complement(550..3201)
                     /locus_tag="VYO63_001274"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1672193.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVIAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(3194..3772)
                     /locus_tag="VYO63_001275"
     CDS             complement(3194..3772)
                     /locus_tag="VYO63_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1672194.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAEILQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(3772..4332)
                     /locus_tag="VYO63_001276"
     CDS             complement(3772..4332)
                     /locus_tag="VYO63_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672195.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHIFPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLDFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(4319..4767)
                     /locus_tag="VYO63_001277"
                     /pseudo
     CDS             complement(4319..4767)
                     /locus_tag="VYO63_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(5179..5574)
                     /locus_tag="VYO63_001278"
     CDS             complement(5179..5574)
                     /locus_tag="VYO63_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1672196.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQPAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(5670..5924)
                     /locus_tag="VYO63_001279"
     CDS             complement(5670..5924)
                     /locus_tag="VYO63_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1672197.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(5934..7268)
                     /locus_tag="VYO63_001280"
     CDS             complement(5934..7268)
                     /locus_tag="VYO63_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1672198.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYEYMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(7268..7501)
                     /locus_tag="VYO63_001281"
     CDS             complement(7268..7501)
                     /locus_tag="VYO63_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1672199.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            7532..7609
                     /locus_tag="VYO63_001282"
                     /pseudo
     CDS             7532..7609
                     /locus_tag="VYO63_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7950..8132)
                     /locus_tag="VYO63_001283"
     CDS             complement(7950..8132)
                     /locus_tag="VYO63_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672200.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(8134..8913)
                     /locus_tag="VYO63_001284"
     CDS             complement(8134..8913)
                     /locus_tag="VYO63_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672201.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         2539 a         2058 c         1720 g         2706 t
ORIGIN      
        1 tttttttcaa tgccatccca tctgtctgtc gaagaacttc caatatattt atttttgggt
       61 aatctttcca tctcatattg tttttgagga gcatatggtt cccaataatc taatcctttt
      121 tttgttccag aacggccttt aagaacttct acatttctag aagctttaac gttataatat
      181 gaatagatta aacattgttt cccatccact tcatctattt gatcaacatt tgtactaaac
      241 caatattcag acacactttt attggctgga gaaccatata aagcttgtaa aattgaagtt
      301 ttatttactc catatctatt acagacacct caggattatt taacttataa gttttaacag
      361 ctacggaatc aatttcaaca gcaacttgaa catctatgcc tgatttttta aggccacttg
      421 tagtgccacc tgcaccgtta aataaatcaa tagcaacaat tttccccata gtattctcct
      481 aaagtttctc ctttttatta taacattatc aaatgtaaaa cccaacccga tagggttagg
      541 tttttaacat catttcacca acttcttcat ctcatcaata cgtgctacgg tcgcatcata
      601 cttggcttgg tagtcggctt gtttgtcttt ttctttttgg acaacttctg gtttagcgtt
      661 ggcgacgaag cgttcgttgc tgagtttttt gccaaccatg tcgagttctt tctgccattt
      721 ggcaagttct ttttccagac gtgccaattc ttcttcgaca ttcaagaggt ctaccagtgg
      781 caagaagatt tcggctcctg taataacgct tgacatagcc agttcaggtg cagggatggt
      841 tgatgcgatt tccaagtgtt ctggatttgt aaagcgtttg atatagttga cattgctgtt
      901 aaagaaggct tccaagtcgc tatcgcttgt cttaacaagg atggtgatag gtttgcttgg
      961 tgctacattt acttccgcac gcgcattacg aacggcacgg atcaagtctt tgagactttc
     1021 cacaccagtg tgggcagcaa ggtcctcaaa ggctgggttg acagttgggt atgctgctgt
     1081 cacgatagag ccttcagcgt actgaccaaa gatttcttct gtcacgaacg gcattattga
     1141 gtgaaggaga cgaaggattt tatccagcgt atagaggaga acagatcgag taattacctt
     1201 atcgtcttca ttgtcgctgt aaagaacttc cttggtcaac tcaacatacc agttggcaaa
     1261 ttcttcccaa atgaagttgt aaaggatatg accagccaca ccaaactcga acttatcaaa
     1321 gttttcagta acttttgcaa tggtttcgtt gagattgtgg agaatccagc ggtccgtcac
     1381 attaccagcc tcacctgttg caacttttgt gacattgtca tgcgccacat ccagcgtcaa
     1441 acctccattg ttcatgagga tatagcgaga aatgttccaa atcttgttaa taaagttcca
     1501 tgaagcatcc attttctcat aagagaaacg cacgtcttgt cctggcgcag aaccgtttga
     1561 aaggaaccat cgaagggcat cggcaccgta tttctcgata acatccattg ggtcaatccc
     1621 gttaccgaga gacttagaca tcttgcgtcc ttgctcgtca cgaatgagac cgtggataag
     1681 gacgttttgg aatggttgac ggcctgtgaa ttccaatgac tggaagatca tacgagacac
     1741 ccagaagaag atgatgtcgt aacctgttac caaggttgaa gttgggaaat aacgtttaaa
     1801 gtcttctgag tcgacttcag gccagcccat ggttgaaaat ggccagaggg cagaactgaa
     1861 ccaagtatcc aagacgtctt cgtcctgagt ccatccgtca ccttctggag cttcttcgcc
     1921 gacatacatt tcaccatcag cattgtacca ggcagggatt tggtgacccc accaaagctg
     1981 acgagagata acccagtcgt ggacattttc catccattga aggaaggtat cgttgaaacg
     2041 aggtgggtag aattcgacct tgtcctctgt gtcttggttg gcaatggcgt tcttagccaa
     2101 ttggtccatc ttgacgaacc attgagtaga caagcgtggc tcaactacga cacctgtacg
     2161 ttctgagtga ccaacactgt ggacacgttt ttcgattttg acaagggcac cgatttcttc
     2221 caacttagca atgactgcct tacgagcttc aaaacgatcc atgcctgaaa attcaaaggc
     2281 aagctcattc atagttccgt cgtcgttcat gacgttgact tgtggcaagt tatgacgttg
     2341 gccaaccaag aagtcatttg gatcgtgggc aggtgtgatt ttcacgacac cagtaccaaa
     2401 ctcaggatct gcgtgctcat ctccaacgat tgggatgagt ttattagcga ttggaaggat
     2461 gacgttttta ccaatcaagt ccttgtagcg cgggtcttct ggattaaccg caaccgcaac
     2521 gtccccaaac atagtctcag gacgagttgt agcaacttca agggcgcgtg aaccatcttc
     2581 cagcatgtaa ttcatgtggt agaaggcacc ttccacatcc ttgtgaatca cctcaatatc
     2641 agaaagggct gtgcgagctg ctgggtccca gttgatgata aactcaccac ggtagatcca
     2701 gcctttcttg taaaggtcca caaagacctt acgaacagct tttgacaaac cttcgtcaag
     2761 agtgaaacgc tcacgagaat agtctacaga gagccccatc ttgccccatt gttccttgat
     2821 ggtagtggca tattcgtctt tccattccca gactttcgtc aagaaagact cacgaccaag
     2881 gtcatagcgg gaaatgccct caccacgcaa gcgctcctca accttagcct gagtcgcaat
     2941 accagcgtgg tccattcctg gaagccaaag cgtatcaaaa ccttgcatgc gtttttgacg
     3001 gatgataata tcttgcaaag ttgtatccca agcgtgacca aggtgaagtt tacctgtaac
     3061 gtttggtggt ggaataacga ttgaataagg cttagccttt tgatcgcctg aaggcttgaa
     3121 aacatcagca tcaagccatt tttggtaacg accagcctca acctcggctg gattgtattt
     3181 agatgaaagt tctttagaca tgtgtgtgtc ctttctctat tttgtttatt ttattttgaa
     3241 tttgcttagc agcttcttct gcagacaaat tcgtattatt tattttaaag tagtggtgca
     3301 actcattcgg ttgatattgg gaatttaatt gaagtatttc agcggtctct ataatttctc
     3361 tttcagatac ctcaatatgt cgttttaagg gtttgtactt taatcgattc tccgttcgat
     3421 ttcgacgtat gcactcttca agacttgttt ccaactcaac aaaaagaatc tcttgatgat
     3481 aatcatccaa caaactctga atttgcttta aatatatcag atgatactga tttgaaaaat
     3541 caattacatc tgttaaaatt actgatcgct gatttcttgc actcgctcca aggaatgaaa
     3601 aagtgattcc acgaacaaat tcccacattt cctctgtata gtcctgatag atttctagtg
     3661 caaaatcgat ggcttgatgg ttataaaata gggtagcatc cgtcagtcga gataattctt
     3721 gaccaatggt catttttcct gatgctggag caccaatgat gaaaagatgc atcaaatcac
     3781 ctcccattca ctcttcagca aggcatatct taaatcatca cagcagttgc cttgagcatc
     3841 tttgcggtct cttatgcgag cttcgagggt aaagtcaagc ttttccgcga ctcgtttact
     3901 ttgaaggtta tatccaaagc aagttagttc aatcttgtga agacccaaat ctttaaaggc
     3961 taagtcaatc aaggcacgcg cagcttctgg cacataacct cgcccccaat agtctgggtg
     4021 taaggtatag ccgatttcca gcacatcatc ttcatggcga tggttgaaat cgacagagcc
     4081 aacgatctta tccgttcctt tgacgacaat cccgtagcct gctgggagat tttccttttg
     4141 attacgctcc ggaaaaatgt gctccagata ataaatctca tcttccaagg tcttgactgg
     4201 aggaaaacct gctggatagg cgacctctgg caaactagca tagtcaaaga tatcctcagc
     4261 atccgccaca gtacggactc gtaaaacgag acgttctgtt tcgattttat ctggcagctc
     4321 agttcttgcc atccttcttc ctcgcttttt tgatgaaact gcccttcata tctacacgct
     4381 tgtccagata gcaataaacg cgctgatatc catctcccat gaaataggtt gggcaaacag
     4441 ttgattttta aaatgtccct tttcatccag gagttctggg gcaacaagtc gctcaagaat
     4501 cttggcaaag atgtggcaaa taccgtattc ctcaacaatc ctatctaccc gataatctaa
     4561 aacaagtgga caggcgtcta aaataggagt ctgagttcgt tcagaaattt cataatgcac
     4621 tcccaaacgt tccaatttct cctgatgact gataaaacca gcctgctcca tcgcaagcat
     4681 agaagtttca tcagaaatat tcacagtaaa tttttgatat tgtttgatct gctctgcggc
     4741 attctctctc gcaacgactc caatcacaac ccaatctcct agactataag aggaactaca
     4801 ggtcatgatg ttatagctaa aattctgatc ttgatatcct aaaataaaaa caggaaaacc
     4861 ataatatagt ttattagttt taaatgattg ttttataaca acctcctttg actaagacac
     4921 aaaagaaaag ccctgccatc tacatgacag ggacgaatgt gtttatccgc ggtaccaccc
     4981 aatttcgggc caagcccgca tctttagtct ttaaaataaa aagacagaaa tatttcattt
     5041 tttcagtcat tagcaaccag ttttgacaca tttgtggact tctcagcgcc gccactttct
     5101 gtaaaatgtg ggattcaaaa cacctctaat ggctctattg tagcaagttt ttctcggaaa
     5161 tgcaaggctc aagaaaaatt acttgaactt gatgccattt tctttcaatt tcttgatggt
     5221 agctgggccg attcctttca aggcaaggag ttctttttca gtccagtttt tgaaatctga
     5281 agcagacttg attccttcat catagaaagt cttggcacga tcaagtggaa gtccccccaa
     5341 gtttgctgca aaatcttcta cagaattagc tactttttga gcttgctctt tggtagcttc
     5401 tactgcttgt tcaacttttt tagaaacagc cttaccagct gcgcttgctg gctgttctgc
     5461 acgcttcacg actttttttg tctcatctac aaccttagtc acagtacttg aaaaggcacc
     5521 tgcgcgacgg aggccatttc gtaattgttt tttacgattg agtttctttg acatgataaa
     5581 atcctttcaa taaaaaatcc ctgctctgac aaggatttaa cataaacatt ctatcaagat
     5641 tttagtgaaa aatcaagaat ctttctcgtt taataatttc gctcaccaaa caagccttct
     5701 ggcaaatcat ggaatccctt gcctgctttg aagttttcaa acttaccaag caagaactga
     5761 taagcatcca agcgactctc gtagcgaatc atggcatctt tggcacgctc gtcctggtct
     5821 tcttcgtaaa ctttttgact ttccttgtgc gccatgtcgt taatcatgat ttgggtattg
     5881 acccaagctt caaattcctt cataaattct tgttcgtaac tcattttttc tccttattct
     5941 aatccacgaa aatagtccgt atcaatctca cggtagggct ggatatcctg aacatactcc
     6001 aacactcctt ggaattctcc gtcttcatcg tgtactgcag cataggtgat gtggacaaac
     6061 ttacctcgcg actcagactt gaaccacatt tcatacttgt ctttgcttcc ctcacgaagc
     6121 cccttcatga tagttttgac cttgtccaag tacttaggcg gatggcagag ttcgacattg
     6181 cgcccgactt gagacggcgt ccgtttgaaa atcatctcat cagctggcgt attgtcattg
     6241 taatactgga aaatatcatc tttattgaca aaggtaatct ccatagggag gtgattgagg
     6301 atgagattgg cctgctcgac tgaaagatag ccattaccaa aagcctgttg actatggcgg
     6361 tccagcacag cttccttttc cttaggggta aaggtaatgg taaaatggcc ttctggcgta
     6421 tctatgactt gttgaacttg accttctgcc gtatctagct gtacaggctc ctctgcaatc
     6481 ttttcctcaa taaagctctg tcgttctggc acccatttct ctgacggacg gatgatggca
     6541 tagccatagg catcgctctc ctccgcaatc tgaagccagt catcctgagt aaaagactca
     6601 aggagaatca tgaggaggat ggactcttcc ttgaaaatca tactttcaaa ctctgtcgca
     6661 aaagcttcaa aagcttcctt tacactgcta attgacactt ctggtagtga cttggctgtc
     6721 gttagagctg tttgaaagag ttccctaatc tgatcatcca ctccccacat aactttggga
     6781 ggtgaatcgt gtccatagcg ctccatgata ggaaagaaga gttcttcctt acgttggtaa
     6841 tggatgtcaa attgacccac aagtcccatc tgacgcacca aacccttacg catctccgcc
     6901 agcatttcct cgtcttccat atactcatag gtatctaaca atctacgaat gcgaatcaag
     6961 gccgcacgga gagccagatt ttcttctttg aagacacgaa ctgggtgacc tggatgctca
     7021 gtatctgaaa cttcgacacc tttgatagca tttttaaaaa gattggcatg gacatcacag
     7081 agttccataa catcttcaaa ggtgacacct gagtctgagt tcatcagctc gtgctccata
     7141 agggaaatct cgatggctga gacacccgca aaggtcgcat caaagcgctc ttgaaccgac
     7201 tcaggagagg cgccattgtg caattctaac aaaatatccc gtaggacatg aatccgttca
     7261 tctgccatta gtctaatcca atcacttcgt agccattcgc ttccagtgtg cgtacaatct
     7321 tgtccatagg agttcctgct agcttagaac cctgtttgag cgataccttg cgaccaactg
     7381 tattgcgcat taagggattg gcaaggggtt taaaacccaa ctccactaga atttccaaga
     7441 cttctggatg cttgtccacc acttctgcaa caggaattga cacatcgatg atattgtcca
     7501 tgacgacctc cattgtatgt tctaatactc aatgaaaatc aaagagcaaa ctaggaagct
     7561 agctgcagac tgctcaaaac agtgctttga ggttgtggat agaactgacg aagtcagctc
     7621 aaaacaccgt tttgaggttg tagatagaac tgacgaagtc agtaaccata cctacagcaa
     7681 ggtgaagctg acgtggtttg aagagatttt cgaagagtat aaaatgattt tactgcttat
     7741 attataccat atggagagag attaaaaaac tagcagtggg gttcctgcta gttttctgcg
     7801 acttggatta ccatctagat aagtttaaag aaccaatcca atatcttgta aaacagcaat
     7861 tgaactgcaa aagaaatgat catccaaaag aatttcacaa tcccaataat cggtttgata
     7921 aaaagaatag caagaagacc aaaaaaattt cattctctct cctctggctt aatgagccac
     7981 cgagccgtat ccattagctt atctgcaata aaaagtttgc ccagaccaac aacgacatgg
     8041 tcagctttct ttatcgtttt catcactttt acaccttgca cggtcaaaaa gtaattacca
     8101 taaattttag ctagagtttt tatgagtccc atattactct ccttcgatga tttcagcctc
     8161 ttttcggatt gtttcaacat cttcttgata ttgaacttca ctatagttaa ctagcttgga
     8221 caattctttc tgcaagcttt cacccatcgg tttcatagtt gcaaaggtta aaccacctga
     8281 aatgatacca cccaagatag gaataaattt ccccattcct ttggccagcc ctcccttggt
     8341 aagattcaca ccaaatattc ttaagacttt tttcaaaata ggataccaaa gcgtcttagt
     8401 taaagcttta ttaggtactg ttttcattac ctgtttggca attgttatac caccagcacg
     8461 tagcaaagca gcagttccat tcacccccaa catgacgcct agataaagca agagggtatt
     8521 ttgagcatct tcactcaact catcgcgtga agcccaaaga tcctcataac cataaatata
     8581 acctaattct tgggccaatt tcagagagaa agcataaaat tgagccacat cagctggaat
     8641 ggtaattgcc atagcaagcc ctccaggtaa tcctgccaaa acagaagtcc cactcgctaa
     8701 taaaacatta tccctaatac aggcattagc cactcgatct aagatttctt gagataagag
     8761 agacgttgga ccttgttcca ataaggtagg aatgtcctgt ggacccaatt ctttggaaaa
     8821 cttatccact aaaaatttcg aacgatcaac cttaacaact ggtagtttta ccacttgttg
     8881 taatacttgc atagctaagt cttgttcagc catatacaaa ctccttattt cttttttaca
     8941 ccttatcata ttatatttcc tacaaaaaaa gcattttcta attagataac attaaaaaat
     9001 acagaacccc tttttttata gta
//
LOCUS       DAVLYR010000072         8952 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLYR010000072 DAVLYR010000000
VERSION     DAVLYR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.072"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(413..2692)
                     /gene="pulA"
                     /locus_tag="VYO63_001285"
     CDS             complement(413..2692)
                     /gene="pulA"
                     /locus_tag="VYO63_001285"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1672202.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPYGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(2804..4762)
                     /gene="ligA"
                     /locus_tag="VYO63_001286"
     CDS             complement(2804..4762)
                     /gene="ligA"
                     /locus_tag="VYO63_001286"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1672203.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERGNLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(4855..6033)
                     /locus_tag="VYO63_001287"
     CDS             complement(4855..6033)
                     /locus_tag="VYO63_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1672204.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGFIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSIHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(6113..6976)
                     /locus_tag="VYO63_001288"
     CDS             complement(6113..6976)
                     /locus_tag="VYO63_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1672205.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYFFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(6979..8880)
                     /locus_tag="VYO63_001289"
     CDS             complement(6979..8880)
                     /locus_tag="VYO63_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1672206.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGDELDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSKKTSGNVVLTVENTAI
                     GYDGEVLSQPINVDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYIEKRATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         2692 a         1978 c         1613 g         2669 t
ORIGIN      
        1 aatccactac gtcaaggcga ggctaatgtg gtttgaagaa attttcgaag agcgtgaatg
       61 agtatcatct atagtaaaat aaaaaaactg aacaatttgg ttggggacag ccaaaccaat
      121 ttctcacaat gtttcagaaa caagggtgtg ctactccaat ttcagcctac tataactgtc
      181 atagattgct gaaacaaagt ctaggtaaaa gtcttcataa taaaaagacc tcctatcaag
      241 tgttcaaaaa ctttgatagg aggtcttgtt ttgtgaaaat atttatcaaa ttttctatac
      301 aagtgagctg ttagccaggt tctttctatt ctttcaattt caatgaatgg attttttact
      361 aatactcata actgggaatt tgtctgtgta aaaatagcga gatagatggt atttataaaa
      421 cactcaagac agctagacta atatcattta aaacattatc ttcttttgag cgactgttgg
      481 ttaccaacat agctaaattt cctgcatttt caaattgata gggttctgat ttagcattca
      541 caaccaccaa gaggtgttct ttgccgtgaa cttcatagat aaggtagccg ctatgttcaa
      601 tcgcagaatg cacaaagaca tgatggtaaa tttcatcata gctagagtaa gaaaaggcac
      661 cagtttttgt cttcaatcgg atgacttgac ggataaactc aatactgtct tgacgctcat
      721 taatcaagtt ccagttcact tggttcacac tgtcaggagc attatagcta ttcatcgcac
      781 gctctctatc atcatgggtc aactcaccat tttcaccagt cgcaaccagt ttggtacgac
      841 caaattcttg accgatttcc ataaaggcca tcccctgcat gagcagattc atggctgtgg
      901 cagtttcgac cttgcgcatg atttgctctg aactttggtc tggatgaagg gttgccaata
      961 aatcgtgaag attgtaattg tcatgggctt ctacatagtt aagcacctga tttggatgtg
     1021 tatagcttcc taattcacga cttcctagga ttgctttagc tagaattggc tctgtcgcag
     1081 caccactgac aaaacctgac ttgatagcac cataaacttc tccccctttg acagcatcgc
     1141 gctgattgtc attaaagaaa ccaatatttg gcatctggta ggcattgtcc ttcttggcct
     1201 tatcataagg ggcaagacct gttcccatat cccatccttc tccatagagg ataatgttgg
     1261 agtcgatttc atccaagctt tgacgaatca tctgcatggt cttgacatca tgaatcccca
     1321 tcaagtcaaa acggaagccg tcaatattat attcctgcac ccagtataga agagaatcaa
     1381 tcatatactt gcgaaacatt tcgtgttcac tggctgtttc atttccaaca cccgttccat
     1441 tctggaaggt accatatgga ttcatacgat aatagtaatc agggactgtt gtttggaatg
     1501 gtgcatcaac aactgagaag gtatggttat agactacatc cataatgact ccaatacccg
     1561 catcgtgata agcttgaacc atcaccttca aatcacgaat gacctgagct ggatcatctg
     1621 gattagttga aaaactagtt tctggcgcgt tatagttttg tggatcataa ccccagttgt
     1681 aggttacatt tccatcctca tcgtattctt tatgacggtc tgcaattggt tgcaattgaa
     1741 cataattgta gcccagcttc ttgatgtaat caaaagcagt tgactggccg tattggttaa
     1801 ctgttccagc ctgagcagca cccaagaaag ttcctcgaag atgttcatct acacccgatg
     1861 taggtgattt agtcaaatca cgaatatgca tttcacagat aactgcctta catggatttt
     1921 ccaagcgcca agtagcctcc gaaccgtgct taacctcgaa gttttcaact tgcttttcta
     1981 catggctcag aatagctgaa cgtttgccat cagggctggt cgcgattgta taaggatcac
     2041 gtgtcagtgt ttggtgatga gggaattgga cttgatactg ataagcctta cctaccaaat
     2101 cttcctcaac atccaaactc cagacaccga ttgtattgtc cttatgatta taagagtagc
     2161 tattgcctct tttcatctca aaagtcttcc aaacgggtgc atcattagca gctgattcat
     2221 aaacgacaac ttgcacttct gtcgctgtag gtgaccagag ggaaaaatga gcctgattgt
     2281 cctctacacg gcaacccaat tctccttggt aaccccaatg atggtcaaaa ctagcactgt
     2341 taatggcctt atcaaaggca aaaggatttt gatttttata gaaaggactg gcaatagcag
     2401 gattttcaga gtaataaatc ctatcatcgc cttccaaaat ccagacctct gttaataggg
     2461 gatagtgatt aaaacggata gaatattctt tactagtttg acctgtatga accacaaaat
     2521 tcaagctttc tataacatgt gaacttgggt gttcaaagct aaataaagct ccaaaataat
     2581 cttctttgta ggttagcaaa tcaattcgtt gatcctgact ttttacaaag gagcaagtgt
     2641 catattctcc attcttacga tggtaatgaa tgcgcatagg gtagttatac attttttatt
     2701 tttccttttt actttgtttc tatttcacta ataaattttt gtcaatctcg tctcaattaa
     2761 cagacatagt catattctct aaactctgtt tttaaacgat ccattacaaa ctttctagcc
     2821 atgcctcatc tctgacctgg ataccaagtt cttgtgcttt ttgcagttta cttccagcgt
     2881 ctgcacctac cacgacgagg tcggtctttt tagaaacact acctgtcact ttggcaccca
     2941 gactttcgag tttactttta gcttctgagc gcttgagtcg ttccaatttt cctgtcaata
     3001 ccacggtcaa acctgacaag gccgcatccg ctactaccgt ctgtccttta tagtccagat
     3061 tgaccccagt ttctttcaat tctctgagca gaatttcaga gccttctgtc gcaaaataag
     3121 tctgaagact tttggcaatc acgccaccta gactttcaat actagccact tcctctgaat
     3181 ctgcctgata cagattttca attgaatgga aatattgaag taaaagctga ctagccttgc
     3241 ttccgacatg acgaattccc aaaccaaata agagcttctc ggcagaattt tcctttgatg
     3301 cttggatagc ctgatacagt ttagcagcgg acttttcctt aaccccctct aaaaggagga
     3361 aatcctcttc ttgcaaacga taaatatccg ccacatcctt gactaaatta gcagcaaaaa
     3421 gcttctcaac aatagatgga ccaaggcctg taatattcat agcatcacga gaagcaaagt
     3481 gaatcaagcc ttccatgatt tgagcagggc aacgcggatt gatacaacgt agggccactt
     3541 catcttcaaa gtgcaacaag tcagagttac aacttggaca gtttgtaggg atatctagtt
     3601 tttcttcaga aacccgtttg gactctacca cacgtaaaac ggcagggatg atgtcaccag
     3661 ccttatatac aatgaccgta tcgtcttttc ggatatcttt ttcagcaata taatctacat
     3721 tgtgcagggt cgcacggcta acagtcgtac cggcaagttg tactggtgtt agattagcag
     3781 ttggagttac aacaccggta cggccaactg tccagtcaac tgataagagt tgagcttctt
     3841 tttcttcagc agggaacttg taggctactg cccactttgg agccttaacg gtaaaaccaa
     3901 gttcttcttg acttgctagg tcgttgacct tgattaccac tccatcaata tcgtaaggca
     3961 gatttccccg ttcctgtcct acttcttgga taaaattcca gatttcatct atgttttcag
     4021 ccaagattcg cttaggattg accacaaaac ctagttgttc taggtacttc aaaccctttt
     4081 cttggctatc acgagttgaa gggctggctt cttgatagag aaacgttgca agattacgct
     4141 tggcaactac tgctgtatcc aactgacgca gagttcctgc tgccgcatta cgaggattag
     4201 caaattcagg ctctccattt tcttggcgca cttggttaac ttggtcaaag gaagcgcgtg
     4261 gcatgtaaca ttccccacga actgtgatat ctagttcttc tggcaaagtc aaagggatgt
     4321 ccttaacacg cttgaggttt tctgtgatat tttcaccaat tgaaccatct ccacgtgtta
     4381 ccccagcaac caaaatcccc ttttcataag tcagcgagat agataagcca tcgattttca
     4441 gctcacaaat ataggtcgga tgagccactt ccttacgaac acgcgcatca aaagcatcta
     4501 gctcctcacg tgaaaaagca tcctgcaaac tataaagagg atactgatga ctgtattttt
     4561 caaaaccatc taaaaccttg ccaccaacac gatgagtcgg actgtctgct agcacttgct
     4621 ctggataagc agtttctaac tcgaccaact cacggtaaag gcggtcatac tcactgtctg
     4681 aaaccgaggg attatcgctg gtatagtact cagtcgcata gcgattgagc aaagcgacta
     4741 actcattcat tcttttattc ataagaccat tttaccataa aacaagccct cctcacaaac
     4801 gagaagggcg gaaaaaacac ttagtttgaa attatttttg aaactcaagc aaccttatat
     4861 caatttttca aaatgagttc gaacataaat aaacgatata caagacaaga tgataacacc
     4921 acttccaatt atcaggaaag aagagagatg tatacttggc aagactgtca taaatccttt
     4981 tgcaataggc ataaatagaa tagctaaggt aaaaattgta ctcagtactc ttccaagaaa
     5041 ttcgctctca accttggttt gtacttgagt aaaaaagtga atattaaaaa tcgtcataaa
     5101 caattcacaa actaaatttc cagaaaagga aagaaaagtt ggaagtggta atcccatcat
     5161 aaaaactccg acacctgtca aagccagtaa aatcaaaaga ttataaatat tagctttaat
     5221 tttactagct agaagagccc caatgatgaa accaatagcc cccatagtta aaatacttgc
     5281 ataggctcct tctgacccgt aaagctgatt cgaaaaggga agtagaaatt caaaagctgc
     5341 aaaaaagaaa ttaacgctgg aagctaccag caaaaggaag aaaatttctt gctgatgcca
     5401 gatatagtgt aacccatcct tgatatctac aaaaatatct ctcccagtaa aagccttttt
     5461 ctcttgaact tttgcttcct cttttggaag gaaagccact agaacaaaag caatgaaaaa
     5521 agtcagcgag tctagcagta gcgtcatatg gagacttgca aactgtaaaa caaggaagga
     5581 aagaacagga gagctaacac ctacaacctg caaaaccagc tctaagcgag aattatagat
     5641 cacaatctca tctttctcca ccacttcagt tatgatagct ttattggctg tgcgagaaaa
     5701 ggcaaaagca atagcctgca caatgttagc aacaatcaaa gcgccaatca tccagctatc
     5761 attccttatg aaagaaatag ccagacaaag aatcccacaa acaagatctg ccgtcattaa
     5821 aatcttacga cgagaaaaac ggtctgaaat aactccgcca aagggattga cgagaataga
     5881 tgtgacgagc tcagaaatct gatacattcc taaaactgtc tgtcctatag tccccataga
     5941 agccaaccag acactatttc cataatcata gagcatattt cccattttat tgatagcccc
     6001 acggctaatc aactgcactg catagcgatt catattaaag ctcctctcaa attttgaaac
     6061 tattgtatca aaaccgaaag gagcttttta ttttttccct tatttgggaa aattaacttt
     6121 tgacaaattt ttcgtagtgt tcctgataat aggctacttg ctctggaaga cctaacacat
     6181 caaaaatatg tatggcctct tgcatctgct tacagccttc tttacactgt cctttttgat
     6241 ataaggcaaa accttttaaa taatggaaaa cattacgctc ataaagctta atacctttgt
     6301 caataatctt ctctgtataa gcctcaaaat agttggcatt ataaaaagaa gaatgctcta
     6361 aacaatgctg gtaacaattg agggccaaaa tcaaaactaa tctcttatgg cgactaatct
     6421 cttggtaaaa ttcctccctc tccataactt ctctaccaat ccgagtgaca tagtctacat
     6481 cgtagaaact atagaggtta ccgaaaagaa tcaactcata catggtccat tcttctgttt
     6541 tgaagaaata atctgctacc ttatccaaat catcctgctt catatcataa ctcgcatctc
     6601 tttgacaaat cagaccttgt agcaaaatcc agttcagctc aaaataaagg ggagtcgtcg
     6661 aactcttaga cttttcaagt tgttctcttt gaagcttttg aaaacctgca atatcgtttg
     6721 aatagtaaag tgggataatc tgtgccatca tagacacatg ttcatgatta tgaaaattcc
     6781 ttgccttatc catgaaattt tcgattgtta catgaatgtt atccaaaatc tcaaagaaac
     6841 gggagactgc caggtcagac tccccaagct caaagcgaga taactgagag gtagagcagg
     6901 attcgcctgc tgcttccttt aaagaataat ttccacttgt tcgaaattca cgaaatactt
     6961 ttccaagatg ttccatcttt acacctgatc tgataattct tcccactcaa gcatagcttc
     7021 ttcctgacga tggctgattt tgtccagctc agcctgtaat tccatgagtt tgtcggcatc
     7081 gtttgtttcc aacatttgtt cagaaatggc ttggctttga ctttctagct cttcaatttc
     7141 agcttctaga ctttcgattt gtcgcatgag tttgcgaact tctttttgac tttctttctg
     7201 ggcctgatag tcattgactg gacttgcttc ctttgcttga ttgctagttg aagcttcctc
     7261 agtctgactc atttctgctg ttgctctctt ctcaatatag tagtcgtaat ctccaaggta
     7321 gagagttgaa ccattctcag acaattccaa aacatgagtt gccacacgat tgataaagta
     7381 acgatcatga ctgacaaaaa gcaaggtacc atcaaagtca atcaaggcat tttctagcac
     7441 ttccttacta tcaatatcca agtggttggt cggctcatcc agaatcaaaa agttattgtt
     7501 ttccatagac aatttagcta aaagcaaacg agctttttcg ccaccagata gcatgccgac
     7561 tgatttttta acatcatctc ctgagaaaag gaaggctcca agacggttgc ggatttcaac
     7621 ttctggtgtc agtttgaaat cattccagag ttcatccagc accgtattac ttggtgtcag
     7681 cttgctttgg gtttggtcat agtaaccaac ctcaacatta gcgccaaagc gcttttctcc
     7741 cttgataaaa ggaatctggt ccacaataga cttgataaag gttgacttgc cgataccatt
     7801 tggaccaacg atagcgacag cattcatctt acgaagatct acgttaatcg gttgtgacag
     7861 gacttccccg tcataaccaa tagcagtatt ttcaacagtc aaaacaacat tgcccgacgt
     7921 ttttttagac tggaaggtca tgttggctgc tttcttgcca gcttcaggct tgtccaaacg
     7981 ctccattttt tctagttgtt tacggcgaga ttgagcacgt ttagtcgttg aagcacgaac
     8041 tagattgcga ttgacaaagt cttccagagc agcgatttcc ttctgttgct tttcatagtt
     8101 ttttgcctca gtagctagct tttgctcctt caattcgaca aaacgagagt aattccccac
     8161 atagcgatcc aaggaatgct tggtcaaatc tagcgtaatt gtcgcaacct tgtccaagaa
     8221 ataacggtcg tggctgacga taatgagggc accgctatag tttaccaagt aattctctag
     8281 ccaggcgatg gtttcaatat ccaagtggtt agttggctcg tccaagacca agagattggg
     8341 cttttcaagg agcattttag ctagagccaa acgagtattt tgaccacctg aaagttcagc
     8401 tattttcatc tgccacatag actcgtcaaa cttgaatcca ttcaaaatcg ctcgaatatc
     8461 agcttcatag gtaaagccgc ctgcttggcg aaaattctca gataagtggt cgtaatctga
     8521 catcagttta tccaattcat cgccagactt ttcacccatc tccaactcca tctgacgaag
     8581 ttgtttatct gtccgacgta agtcatcaaa gacatgaagc atctcatcat agatggtatt
     8641 ttcagactca aaacggctat cttgagctag gtaagacaga gaaatatctt ttttcttatt
     8701 gatttctcca ctagttggct cctcttctcc aactaaaatc ttcaaaagag tagacttacc
     8761 tgcaccattt ttcccaacaa gggcaatccg atctcgttca tcaacctgca ggttgatatt
     8821 atcgaaaaga acctctcctg caaaagaacg ttcaatttta ttagcttgta aaataatcat
     8881 acaagtagta tagcatgttt ccctaagtca ttcaagataa tcgtaagtct tttagtacaa
     8941 cttttataac at
//
LOCUS       DAVLYR010000073         8799 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLYR010000073 DAVLYR010000000
VERSION     DAVLYR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.073"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            59..3160
                     /locus_tag="VYO63_001290"
     CDS             59..3160
                     /locus_tag="VYO63_001290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1672207.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQKSEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQVDD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3242..4249
                     /gene="pfkA"
                     /locus_tag="VYO63_001291"
     CDS             3242..4249
                     /gene="pfkA"
                     /locus_tag="VYO63_001291"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1672208.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     L"
     gene            4308..5813
                     /gene="pyk"
                     /locus_tag="VYO63_001292"
     CDS             4308..5813
                     /gene="pyk"
                     /locus_tag="VYO63_001292"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1672209.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6547..6772)
                     /locus_tag="VYO63_001293"
                     /pseudo
     CDS             complement(6547..6772)
                     /locus_tag="VYO63_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7620..8492
                     /locus_tag="VYO63_001294"
     CDS             7620..8492
                     /locus_tag="VYO63_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1672210.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            8505..8669
                     /locus_tag="VYO63_001295"
     CDS             8505..8669
                     /locus_tag="VYO63_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672211.1"
                     /translation="MTVILGFLFLFLMTWWVISIVNSQKPLKNETFIGYVQRYDIDGN
                     GYQSLPCPQN"
     gene            complement(8638..8793)
                     /locus_tag="VYO63_001296"
                     /pseudo
     CDS             complement(8638..8793)
                     /locus_tag="VYO63_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2661 a         1560 c         1898 g         2680 t
ORIGIN      
        1 agccatttat aggtgaaaat ggtataatat agtgagaagg atagaggaga agtgtaaatt
       61 gatcgcacaa ctagatacaa aaacagtcta tagttttatg gaaagcgtca tttcgatcga
      121 aaagtatgtg agagcagcta aagaatacgg ctacactcat ttggctatga tggatattga
      181 caatctttat ggcgctttcg actttctaga gattacaaaa aaatacggca ttcatccttt
      241 gctagggctt gaaatgacag tgtttgtaga tgatcaggaa gtgaatttgc gctttttagc
      301 tctatctagt gtgggctatc agcagttgat gaagctttcg acagccaaga tgcaggggga
      361 gaaaacttgg tcagtcctgt cccagtacct ggaggatatc gcggtcattg tgccttattt
      421 tgatagagtt gagtcgttag aactaggctg tgattactat ataggggttt atccagaaac
      481 actagcaagc gaatttcatc atcctatctt acctctttat cgggtcaacg cttttgaaag
      541 cagggataga gaagttcttc aagttttaac agcgattaaa gaaaatctac cgctcagaga
      601 agttcccttg cgttcgagac aagatgtctt tatatcagca agttctttag agaaactatt
      661 ccaagagtgt tttccgcaag ctttggacaa tttagaaaag cttatttcag gcatttctta
      721 cgacttggat actagtctga aactgcctcg ttttaatcca gctagaccag cagtagagga
      781 gttgagagag cgtgctgaac tggggcttgt tcagaagggg ttgactagta aagaatatca
      841 agatagacta gaccaagaat tgtctgttat tcatgatatg ggctttgatg attatttctt
      901 ggttgtttgg gatttgttgc gttttggacg atcgaatggc tattatatgg gaatgggaag
      961 gggttctgca gtaggcagtt tggtttctta tgccttagac atcacgggga ttgacccagt
     1021 agagaaaaat ctgatttttg aacgctttct taatcgtgaa cgctatacca tgcctgatat
     1081 tgatattgat atcccagata tttatcgtcc agattttatc agatatgttg gtaataaata
     1141 tggtagtaaa catgcggcac aaatcgttac tttttcaacc tttggagcca agcaagctct
     1201 tcgagatgtc ttgaaacgct ttggtgtgcc agagtatgaa ttatctgcaa ttactaagaa
     1261 aatcagtttt cgtgacaatc ttaagtcggc ctatgagggc aatctccagt ttcgtcagca
     1321 aatcaatagt aagttagaat accaaaaagc ttttgagatt gcttgcaaga tagagggcta
     1381 tccaagacaa acctctgtcc atgcggctgg tgttgtaatt agtgaccaag atttaaccaa
     1441 ctatattcct ctaaagtatg gtgatgaaat tccactgact cagtatgatg ctcatggagt
     1501 tgaggctagc ggacttttga agatggactt tctgggacta cgaaatttga cctttgtcca
     1561 gaagatgcaa gagttgcttg ctgaaataga aggtattcac cttaaaattg aagaaataga
     1621 tttggaagac aaagaaacgt tagctttatt tgcctctgga aatacaaaag gtatctttca
     1681 atttgagcaa ccaggtgcca ttcgtctgct taagcgtgtg caaccagtct gttttgaaga
     1741 tgtcgtcgcg actacttctc taaatcgacc gggtgctagt gactatatca ataattttgt
     1801 ggcaagaaag catgggcagg aagaagtgac tgttctggat ccagtactgg aggatatttt
     1861 ggctccaacc tacggcataa tgctctatca ggagcaggtt atgcaggttg cccagcgatt
     1921 tgccggattt agtcttggga aagccgatat tttgcgtcgg gctatgggga aaaaggatgc
     1981 ctctgccatg catgagatga gggcttcctt tattcaaggt tcattagaag ctggtcatac
     2041 tgtggaaaaa gcagagcagg tctttgatgt tatggagaag tttgcaggtt atggttttaa
     2101 caggtcacac gcctatgcct actcagcctt ggccttccag ttggcttatt ttaaaacaca
     2161 ttatccagcc attttttatc aggtcatgtt aaattcttcc aacagtgatt acttaataga
     2221 tgcacttgaa gcaggttttg aagtagcctc tctatccatc aacaccattc cctatcacga
     2281 taaaattgcc aacaaggcca tctatctagg tttgaaatcc attaaaggag tcagtaatga
     2341 tttagctctc tggattattg aaaatagacc ttattctaac attgaagatt ttatagctaa
     2401 attacctgag aattatctga aacttcctct gctagaacct ttggtaaaag ttggtctttt
     2461 cgattcattt gaaaaaaatc gtcaaaaagt atttaataat ttagctaatc tatttgaatt
     2521 tgtgaaagag ttgggaagtt tgtttggaga tgctatttat agttggcaga aatcggaaga
     2581 ttggacggaa caagaaaaat tttatatgga acaagagctt ttagggatag gtgtcagcaa
     2641 acatccacta caagctattg caagtaaggc tatttacccg attaccccaa tcggaaattt
     2701 gtcagaaaat agctatgcta ttattttggt tgaagttcag aaaataaaag tgattcgtac
     2761 caaaaagggt gaaaatatgg ctttcttaca ggtggatgac agtaagaaaa aattggatgt
     2821 tacactcttc tcagatttat atcgacaggt tggtcaggaa gtaagagaag gagcattcta
     2881 ctatatcaaa gggaaagtcc aatcacgtga cggtcgtctg caaatgattg cacaagaaat
     2941 aagagaagca gttgctgaac gcttttggat tcaggttaaa aatcatgact cggatcaaga
     3001 gatgtcacgt attttagacc agtataaagg cccaatccca gtcatcatcc ggtatgaaga
     3061 ggaacagaaa accatcgttt ctccccatca ttttgtagct aaatctgatg aattagaggc
     3121 gaaattgaat ggaatagtta tgaaaacgat ttatcgctaa aattacggaa aatagaagaa
     3181 ttttcaacgt aaatgtggta taatcagtaa gaatgttaaa agaaaaagga gcataaccaa
     3241 tatgaaacgt attgctgttt tgactagtgg tggagacgcc cctggtatga acgctgccat
     3301 ccgtgcagtt gttcgtcaag caatttcaga aggaatggaa gtgtttggta tctatgacgg
     3361 atatgctggt atggttgccg gtgaaattca tcccctagat gcagcttcag taggggacat
     3421 catttctcgt ggtggtactt tccttcactc agctcgttac ccagagttcg ctcaacttga
     3481 agggcaactt aaagggattg agcaattgaa aaaacacgga attgaaggtg tagttgttat
     3541 cggtggtgac ggatcttacc acggcgctat gcgtttgact gaacatggct tcccagctat
     3601 tggtcttcca ggtacaatcg ataacgatat cgttggtact gactttacaa tcggttttga
     3661 cacagcggtt actactgcca tggacgctat cgataagatt cgtgatacat catcaagtca
     3721 ccgtcgtact tttgtaatcg aagttatggg acgtaacgct ggtgatatcg ctctttgggc
     3781 tggtattgca actggtgctg atgaaatcat catccctgaa gcaggcttca agatggaaga
     3841 tatcgtagca agcatcaaag ctggttatga atgtggtaaa aaacacaata tcatcgtctt
     3901 agctgaaggt gtgatgtcag cggctgaatt tggtcaaaaa cttaaagaag ctggagatac
     3961 aagcgacctt cgtgtaacag aacttggaca tattcaacgt ggtggttctc caactgcgcg
     4021 tgaccgtgtt ttggcgtcac gtatgggtgc acatgctgtt aaacttctta aagaaggtat
     4081 cggtggtgtt gcggttggta ttcgtaacga aaaaatggtt gaaaatccaa ttcttggtac
     4141 tgcagaagaa ggggcattgt ttagccttac tgcagaaggt aagattgtgg ttaacaaccc
     4201 acacaaagcc gatattgagc tatctagctt gaataagagc ttgttataat ttacaattta
     4261 gttaataaga ccaaaaaggt catatatata aaggagtcac aaaaatcatg aacaaacgtg
     4321 taaaaatcgt tgcaactttg ggtcctgcgg tagaaatccg tggtggtaaa aaattcggtg
     4381 aggacggata ctggggtgaa aaacttgatg ttgaagcttc agctaaaaac attgctaaat
     4441 tgattgaagc tggtgctaac acattccgat tcaacttctc acacggcgac caccaagaac
     4501 aaggtgagcg tatggcaact gttaaacttg cggaaaaaat tgcaggtaaa aaagttggtt
     4561 tccttcttga tacaaaagga cctgaaatcc gtacagaatt gttcgaaggt gaagctaaag
     4621 aatattcata caaaactggt gaaaaaattc gtgttgcaac taaacaagga atcaaatcaa
     4681 ctcgtgaagt gattgcgttg aacgttgctg gtgctcttga tatctatgat gatgttgaag
     4741 ttggtcgtca agttttggtt gacgatggta aacttggtct tcgtgtggtt gctaaagatg
     4801 atgcaactcg tgaatttgaa gttgaagttg aaaacgatgg tatcatcgct aaacaaaaag
     4861 gtgtgaacat ccctaacact aaaattcctt tcccagctct tgctgaacgc gataacgacg
     4921 atatccgttt cggtcttgaa caaggtatca acttcatcgc aatttcattc gtacgtactg
     4981 caaaagatgt gaacgaagtt cgtgcaatct gtgaagaaac tggaaacgga catgttcaat
     5041 tgttcgctaa aatcgaaaac caacaaggta tcgataactt agacgaaatc atcgaagcag
     5101 ctgatggtat tatgattgct cgtggtgata tgggtatcga agtaccgttc gaaatggttc
     5161 cagtttatca aaaaatgatt atcaagaaag tcaatgctgc aggtaaagtt gttatcactg
     5221 caacaaacat gcttgaaaca atgactgaaa aaccacgtgc aactcgttca gaagtatcag
     5281 acgtattcaa cgctgttatc gacggaactg acgctacaat gctgtcaggc gagtctgcaa
     5341 acggtaaata cccactcgag tcagtaacta caatggctac aatcgacaag aacgctcaag
     5401 ctcttcttaa tgaatacgga cgtcttgatt cagattcatt tgagcgtaac tctaagacag
     5461 aagtaatggc ttctgctgtt aaagatgcta ctagctcaat ggatatcaaa ttggttgtaa
     5521 ctcttactaa gacaggtcat actgcacgtt tgatttctaa ataccgtcca aatgctgaca
     5581 tcttagcatt gacatttgac gaattgacag aacgtggctt gatgttgaac tggggtgtta
     5641 tcccaatgtt gacagatgct ccatcttcaa ctgacgatat gttcgaaatc gctgaacgta
     5701 aagcggtaga agcaggtctc gttgagtcag gcgatgatat cgttatcgtt gctggtgtgc
     5761 cagtaggaga agctgttcgc acaaacacaa tgcgtatccg cacagtacgt taagaaaaat
     5821 ataaaaacct atcatatcca gctttctagc ctgtatgatg ggctcttttt ttgtccaggg
     5881 ggcaaaaagg gggcaaaact ttcataatgg attgatactc ttcgaaaatc tcttcaaacc
     5941 acgtcagcgt cgccttaccg tatatatgtt actgacttcg tcagttctat ctacaacctc
     6001 aaagcagtgc tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga
     6061 gtatgaaata agatatgcac aaattgatta ggaaattcaa atgaatttct acaaatgttt
     6121 tagcaatcgc aatctacttg tctagattcg atctgatata ttttcgattt aatgatatgg
     6181 tatttaaaac ctccaaagta gcttgctcca ttcttttact tacgtgagtg tagatgttat
     6241 ttactgtttt agcgtttttg tgttctactc taaccattat agcattcttc tcagctagtg
     6301 tactaaggag tgtgtgcctg aaaatatggg aactaagggc tggtttatcg gtttctctag
     6361 tttagtattt gccttttgca aagtgatctt aaatgccttt ctctaaattt acatatcact
     6421 attgtttaac aaaatctaat ctactttagg tcacttattc tttttttgaa atgtagaatg
     6481 aactttttca aagtttttcg aatcttttaa aatctgtttg ttttatatcg ccattctccc
     6541 ccttttttaa ttctccctat atagcctgac aactttcccg atggtacgaa tatggttgct
     6601 ttcgtctagg tggatgtcgg agtattcggg attgagtttt tttgaggtag ccttggcgga
     6661 gtttcttgac atagttagtg ccgtctactt ggaagatgcc gatggtatta tagtcaatct
     6721 gtggggtatt cttgataaat aggtagtcgc tgtttcttat ctttggctcc atggacttgc
     6781 tgacgacata agcgattggg tcgtagtcgt ctgggataat ggaaactcca taactaaatc
     6841 gtcgtcctgc atcaagcggc tacctgcaga gataaactac ctaacacgag agtaagtagt
     6901 ctgtctgtag tcgtccagtc tgatgatttt tacgatactt cgtttttctg atcatacagt
     6961 tgcctctcgg cataggtcag aactttacct tgtctgggtg gtgcccgttg gtcgtagata
     7021 gattggatat cgctaggaga atccttttga actggaggaa agagggcatc aatcaagcta
     7081 ctgaatactt taactaagtc aaatatagta tttttcttag tagacctaac ccttttttca
     7141 taatttctaa tggtgttttt acttatacct atcttagtac ccaattctta ttgagtccaa
     7201 ccattactag tctatattgt tttatagttg attgagtttg gaatagtacg ctgtagctgc
     7261 taaaacattt ctaggaatta atttgccttt cctaatagag ttgttcatat cttatttcaa
     7321 tctactatat ttttcacctc taacaatcgc aatctcttct ttatccatga acgaaaacgc
     7381 tttctatctt tgtaagtaaa gcatcacacg aaatccacga aaatgaaaac ctttgttgtg
     7441 ttttcgtaaa aaatttgttg acagagcacg aaacgcctac tataattaaa tcaagtttaa
     7501 ggaaataaca aaaaaacaaa tcggagggaa agacgatgaa cacattaaat gagaaagtaa
     7561 tcaatatctg taaagcagta gttaaagaaa ctttaatcca agacatttag gagaaagaaa
     7621 tgaagaaatc aagaaaatta gccacattag gaatctgttc agccttgttt ttaggcttgg
     7681 ctgcttgtca acaacaacat gctacttctg aggggacgaa tcaaaggcaa agcagttcag
     7741 cgaaagttcc atggaaagct tcatacacca acctaaacaa ccaggtaagt acagaagagg
     7801 tcaaatctct cttatcagct cacttggatc caaatagtgt tgatgcattt tttaatctcg
     7861 ttaatgacta taataccatt gtcggctcaa ctggcttatc aggagatttc acttccttta
     7921 ctcacaccga atacgatgtt gagaaaatca gtcatctctg gaatcaaaag aagggcgatt
     7981 tcgttgggac caactgccgt atcaatagtt attgtctttt gaaaaattca gtcaccattc
     8041 caaagcttga aaagaatgac cagttgcttt tcctagataa tgatgcgatt gataaaggaa
     8101 aggtctttga ttcacaagat aaggaagagt ttgatattct attttcgaga gttccaactg
     8161 aggcaactac agatgtcaag gttcacgctg aaaagatgga aacattcttc tcacaatttc
     8221 aattcaatga aaaagctcga atgctgtctg tagtcttgca cgacaatctg gatggcgagt
     8281 atctgtttgt aggtcacgtt ggggtcttag tacctgctga tgacggtttc ttatttgtag
     8341 agaaattgac tttcgaagaa ccctaccaag cgattaaatt tgctagtaag gaagattgct
     8401 acaagtattt gggcaccaag tatgcggatt atacaggcga gggactggct aagcctttta
     8461 tcatggataa tgataagtgg gttaaacttt aatagtgtta tgatatgaca gtaatcttag
     8521 gatttttatt tctatttttg atgacttggt gggttatttc aatcgttaat agccagaaac
     8581 cactcaaaaa tgaaacattt attggctatg tccaaagata cgatattgat gggaatggct
     8641 atcaatcatt accgtgtcct cagaactgag aggagttctt gaaatcgtaa caccactttg
     8701 aacaagagtt acttcaaccc attggctccg ccggagtaag ttgctttcgt gaacaccaaa
     8761 atcagccgca atttcttcat aagtgcggta ttctcacac
//
LOCUS       DAVLYR010000074         8781 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLYR010000074 DAVLYR010000000
VERSION     DAVLYR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.074"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(585..1613)
                     /gene="queA"
                     /locus_tag="VYO63_001297"
     CDS             complement(585..1613)
                     /gene="queA"
                     /locus_tag="VYO63_001297"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1672212.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKIVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            1764..2471
                     /locus_tag="VYO63_001298"
     CDS             1764..2471
                     /locus_tag="VYO63_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1672213.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            2611..2787
                     /locus_tag="VYO63_001299"
     CDS             2611..2787
                     /locus_tag="VYO63_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1672214.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            2917..3852
                     /locus_tag="VYO63_001300"
     CDS             2917..3852
                     /locus_tag="VYO63_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1672215.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            3845..4633
                     /locus_tag="VYO63_001301"
     CDS             3845..4633
                     /locus_tag="VYO63_001301"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1672216.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            4634..5017
                     /locus_tag="VYO63_001302"
     CDS             4634..5017
                     /locus_tag="VYO63_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1672217.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            5033..5422
                     /locus_tag="VYO63_001303"
     CDS             5033..5422
                     /locus_tag="VYO63_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1672218.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            5506..6636
                     /locus_tag="VYO63_001304"
     CDS             5506..6636
                     /locus_tag="VYO63_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1672219.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            6641..7378
                     /locus_tag="VYO63_001305"
     CDS             6641..7378
                     /locus_tag="VYO63_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1672220.1"
                     /translation="MCLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            7389..8162
                     /locus_tag="VYO63_001306"
     CDS             7389..8162
                     /locus_tag="VYO63_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1672221.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            8152..8769
                     /locus_tag="VYO63_001307"
     CDS             8152..8769
                     /locus_tag="VYO63_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1672222.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
BASE COUNT         2525 a         1630 c         2013 g         2613 t
ORIGIN      
        1 catcttatag cgactataaa aaagtgaaca agcctagtct tctgtacatt agaattcaga
       61 tttgttcact ttttgtttaa tactcaatga aaatcaaaga gcaaactaga aaactagtcg
      121 caggttgctc aaagtactgc tttgaggttg tagatagaac tgacgaagtc agtcacatat
      181 ataatccaag gtgacgttga cgcggtttga agagattttt gaagagtata agtctttgat
      241 tttgagacta aggagagtca gggtcaaagt agtgaatgat tctgaaataa ctcataatag
      301 tctcttgaga ggacagataa gagtaacaaa tatggtgcta ctcatgtgtc ttgcattttg
      361 atttacaaac agcgaagaaa atataggaag ctgaaataaa atatagatga atcaatttgg
      421 agagtcaacg caatctatgg caatattttc gaagctaggg ggaagcattt taatttcaat
      481 gtactaggcg tcttctacag ctagctagag tttcaattgt agatgttctt tgaaataata
      541 tcttagcgat ttattggtat tagaagaatt agagaaattc tttctcaata aataaacatg
      601 gcgtcaccaa aactgaagaa gcggtagtgt tcttggatgg catggtggta ggcatctaag
      661 actaattcac ggcctgcaaa ggcagaaacc aacatgacca gagttgattt tggcagatgg
      721 aagttggttg agaaggcatc cacgatcttc cactcatacc caggtttgat aaagatattg
      781 gtccaaccag aatctgcttg gatttgccca tcaaacttgg aaccaatagt ttccaaggtg
      841 cggatagaag tggttccgac agcgatgacg cgaccaccat tttctttgac agagcgaagg
      901 gtggcagcag cttcctcaga aagttgatag aactctgagt gcatttcgtg ttcgtccaga
      961 ttatccacag aaacaggtct aaaggttccg agtccgacat ggagagtcag atagactaga
     1021 tgaacaccct tagcttggat ttctgccagc agttctttgg tgaagtgaag accagcagtc
     1081 ggtgctgcag cagagccgct ttccttggcg tagacggttt gataacgttc acggtcatct
     1141 aatttttcgt ggatataagg tggcagaggc atttctccca gactttccaa gacttctagg
     1201 aaaattcctt ggtattcaaa gcggacaatg cgtcccccgt gggtcaattc ttctgtaacg
     1261 acagcgctga ggcggccatc accaaagctg atacgagtac cgaccttgag gcgtttggca
     1321 ggtttagcca gaacttccca ctcgtctcca ctagtgttct taaggaggag aagttccaca
     1381 tgacctcctg tctccacttt ttgaccatag aggcgggcag ggagaactcg ggtgtcgttc
     1441 atgacaaggg catcaccagg ttccagcata tcaataatag agtggaaatg tttatcttgc
     1501 atttctcctg tctcacggtt gacgatgagg agtttggagg catcacgttt ttcaaggggc
     1561 gtttgggcaa tcaattcctc tggcaagtgg aaatcaaaat cagctgtatt catatatttg
     1621 ttcattcttt ctaagttcta atcctatcca ttataacacg tttcaagttt ggacgctctt
     1681 tttttagaaa ttaaatcgtt ttcacttgac aaaaattggt ctataccata taataaatat
     1741 agacagaaat ggaggatgaa aagatgaaag ttattaaagt tgaaaaccaa gttcaaggtg
     1801 gaaaagttgc ttttgagatt ttgaaggaaa aattggctaa tggagctcaa actctaggac
     1861 ttgcgacagg aagtagccca cttgaatttt acaaggaaat tgttgagagt gaccttgatt
     1921 tttcaaatct aaccagtgtc aaccttgatg agtatgtggg ccttgatggg gacaatccac
     1981 agtcttatcg ttacttcatg caagaaaact tgttcaacca aaaaccattt aaagaaagtt
     2041 tcttgcctcg tggtgttaag gacaatgctg aagctgaagt tgaacgctac aaccaaattt
     2101 tggctgacca tccagttgac ctccaaatct tgggaatcgg tcgcaatggg catatcggct
     2161 ttaatgagcc tggtactcca tttgacagtc aaacgcatct agtagaactt gaccagtcta
     2221 ctatcgaagc caatgctcgc ttctttgcca agattgaaga cgtcccaacc caagctattt
     2281 caatggggat taaaaacatt ttggatgcca agtctattat tctttttgct tatggtgagt
     2341 cgaaagcaga agccattgcc ggaacagtat ctggcccagt gactgagaac ctacctgcaa
     2401 gtagcctcca aaatcaccct gatgtaacca tcatcgcaga tgctgaagcg cttagcttac
     2461 tcgaaaaata aaaaagcaat gttctattga acgctttcaa ctcttgtata aatggaagaa
     2521 aaaatcaaaa ttaaaccgca tttttgcttg acaattattc cttttacgtg tagaatgaaa
     2581 tagatattga acttgaaggg agtgaaaaaa atgtctaaaa cagtagtacg taagaatgaa
     2641 tctcttgacg acgcacttcg ccgtttcaaa cgtgcggtta ctaaagctgg tactcttcaa
     2701 gaaacacgca aacgtgaatt ctatgaaaaa ccttctgtaa aacgtaaacg taaatcagaa
     2761 gtagctcgta aacgtaaaaa attctaattg aaatgaaagg ctagagaaat ctagtccttt
     2821 ttcttttaaa taaatactcc aaagcctgca aaaatctgaa acttcctcct acaatttgat
     2881 ataatagaga gaagaattca tttgaaggag gaaatgatgt cggttttagt aaaagaagtg
     2941 attgaaaagc ttagactaga tattgtctat ggtgaaccag aattgcttga aaaggaaatc
     3001 aatacagcgg atattacgcg acctggtctt gaaatgacag gctattttga ctactataca
     3061 ccagagcgga ttcaactttt ggggatgaag gagtggtctt atctgatcag catgccttcc
     3121 aacagccgtt atgaagtttt gaaaaaaatg tttctacctg agacaccagc agtcattgtt
     3181 gcccgtggtt tggtggttcc agaggagatg ttaaaggctg ctagagaatg taagattgct
     3241 attttaacca gccgtgcagc taccagtcgt ttatctggag agttatctag ctatctggat
     3301 tctcgtttgg cagaacgtac cagtgtgcac ggtgtcttga tggatattta tgggatgggc
     3361 gtcttgattc agggagatag tggaattggt aagagcgaga caggtcttga gcttgtcaaa
     3421 cgtggtcacc gtttggtagc cgatgaccgt gtcgatatct ttgccaagga tgagattact
     3481 ctctggggtg aaccagctga aattttgaaa cacttgattg aaattcgtgg ggttggtatt
     3541 atcgatgtta tgagtctcta cggtgcgagt gctgtcaagg attcttcaca ggttcagctt
     3601 gctgtctatt tggaaaatta cgatacgcat aagacctttg atcgtcttgg aaacaatgca
     3661 gaggaacttg aagtttctgg cgtagccatt cctcgtattc gtattccagt taaaacaggt
     3721 cgtaatatct ctgttgtgat tgaggcagct gccatgaatt atcgtgccaa ggaaatgggc
     3781 tttgatgcta cccgtttgtt cgacgaacga ctgacaagtc tcatagctcg aaatgaggtg
     3841 caaaatgctt gatccaattg ctattcaact aggaccccta gccattcgtt ggtatgcctt
     3901 atgtattgtg acaggcttga ttcttgcggt ttatttgacc atgaaagaag cacctagaaa
     3961 gaagatcata ccagacgata ttttagattt tatcttagta gcctttccct tggctatttt
     4021 aggagctcgt ctctactatg ttattttccg atttgattac tatagtcaga atttaggaga
     4081 gatttttgcc atttggaatg gtggtttggc catttacggt ggtttgataa ctggggctct
     4141 tgtgctctat atctttgctg accgtaaact catcaatact tgggattttc tagatattgc
     4201 ggcgcctagc gttatgattg ctcaaagttt ggggcgttgg ggtaatttct ttaaccaaga
     4261 agcttatggt gcaacagtgg ataatctgga ttatctacct ggctttatcc gtgaccagat
     4321 gtatattgag gggagctacc gtcaaccgac tttcctttat gagtctctat ggaatctgct
     4381 tggctttgcc ttgattctga tttttagacg gaaatggaag agtctcagac gaggtcatat
     4441 cacggccttt tacttgattt ggtatggttt cggtcgtatg gtcatcgaag gtatgcgaac
     4501 agatagtctc atgttcttca gccttcgagt gtcccaatgg ctgtcagttg tctttatcgg
     4561 tctcggtata atgatcgtta tttatcaaaa tcgaaagaag gccccttact atattacaga
     4621 ggaggaaaac taaatgttag aagttgcata tattcttgtt gccctagctt tgattgtctt
     4681 tttggtctat ctgatcatta ctgtacaaaa gcttggtcgt gtcatcgatg aaacagaaaa
     4741 gacgattaaa accttgactt cagatgtgga tgtgaccttg catcacacca atgagttgtt
     4801 ggctaaggtc aatgtcttgg cagatgatat caatgtcaag gtggctacga ttgatccact
     4861 cttcagtgct gttgcagatt tatctctatc tgtttcagac ctcaatgacc atgcgcgtgt
     4921 cttgagcaag aaagcttcat cagctggttc aaaaacactc aagactggtg caagtctgtc
     4981 agctcttcgt cttgcaagta aatttttcaa aaaataaaaa aggagaatcc ttatgggtaa
     5041 attatcctca atccttttag gaaccgtttc aggtgcagct cttgccttgt ttttaacaag
     5101 tgataagggc aaacaagttt gcagtcaggc tcaagatttt ctagatgatt tgagagaaga
     5161 tccggagtat gccaaggagc aagtctgtga aaaactgaca gaagttaagg agcaggctac
     5221 agattttgtt ctgaaaacaa aagaacaggt tgagtcaggt gaaatcactg tggacagtat
     5281 acttgctcaa gctaaatcct atgcttttca agcgacagaa gcatcaaaaa atcaattaaa
     5341 taatctcaag gagcaatggc aagaaaaagc cgaagctctt gatgactcag aagagattgt
     5401 gattgatata acagaagaat aaaccatcac catctccgga cggactatgt atctggggat
     5461 ggtgattttt atctggaatc tagtctttgt ggtataataa ttactatgca gaaaaaacca
     5521 acgtcagcct atgtgcacat cccattttgt acccagattt gttattattg tgatttttca
     5581 aaggtcttca tcaaaaatca gccagtcgac agctatttag agcatctgct ggaagagttt
     5641 cgttcttatg atattgaaaa gttgtcaacc ctttatatcg gtggtggaac accgacagcc
     5701 ctgtcggctc cgcaactgga ggtgttactg aatggcttga ctaaaaactt ggacttgtct
     5761 gtcttggaag agttgaccat tgaagccaat ccaggcgatt tggatgcgga taagatagct
     5821 gttttgaaaa attcggctgt caatcgtgtt tcgctaggtg tccagacctt tgatgataag
     5881 atgttgaaaa agattgggcg cagtcatttg gagaaggata tttatgaaaa tatcgatcgc
     5941 ctgaaactgg ctggttttga caacatctcc attgatttga tttatgctct gcctggtcag
     6001 accatggagc aagtaaagga aaatgtggct aaagccattg gactggatat tccccacatg
     6061 agtttgtata gtttgatttt agaaaaccat acggtcttta tgaaccggat gcgacgtggg
     6121 aaattgcctc tgcctaagga ggaactagaa gcggagatgt ttgagtacat cattgcagag
     6181 ctggagcgag cgggttttga gcattatgag atttctaatt tctccaaacc cggctttgaa
     6241 agtcgtcaca atctcatgta ctgggacaat gctgaatact atggtattgg tgcaggggca
     6301 tctggttatg tcaatggagt acgctataaa aatcatggtc ccattcgtca ttatctcagt
     6361 gcggttgagg aaggcaatgc ttgtattaca gaagatcacc tgagtcaaaa ggagcaaatg
     6421 gaagaagaaa tgttcttggg actccgcaag aaatccgggg tttccatggc gcgatttgag
     6481 gaaaaatttg gacagtcttt tgctggactt tatggagaaa ttgtcagaga tttggttcaa
     6541 caaggcctca tgcagattga gggtgaccac gttcgcatga caaagagagg tctctttttg
     6601 ggagatactg tagcagaacg atttattttg gagtaggatc atgtgcttaa cttatcaaat
     6661 gaaaatgaaa attccttttg atatggctga tatgaacggt catatcaaac ttccagatgt
     6721 gattttgctg tcactgcaag tttcagggat gcaatcgatt gaactgggag ttagtgataa
     6781 ggccattttg gaagactata atctggtctg gattatcgca gaatatgata ttgaggtggt
     6841 tcgtttgcct cgttttgcgg aagaaattac catcgaaacg gaagccttga gctacaatcg
     6901 acttttttgc taccgtcgct ttactattta taatgaagcg gggcaggagc tcatccatat
     6961 gatggcgacc tttgttctca tggaccgcga cagtcgaaaa gtccatgctg ttgaacctga
     7021 gattgtggct ccttaccagt ctgattttga taaaaaactt atccgtggac cgaagtatga
     7081 atccttggaa gagccgatca gcaaggatta ccatgttcgt ttttacgact tggatatgaa
     7141 tggtcatgtc aataacagta aatacttgga ctggattttt gaggtcatgg gagcggattt
     7201 tttgacccac tatattccca agaaaatcaa cctcaagtat gtcaaggaag ttcgaccagg
     7261 tggggtgatt acatcggctg ttgaacggac tggactggaa agcaagcatg aaattacaag
     7321 tgatggggtt atcaatgccc aagcaatcat tacttggcaa gaaataaaaa aggtttaggg
     7381 agaaatagat gaaatataaa ggctatttaa ttgatttaga cggaaccatt tataagggga
     7441 aagaccgaat ccctgctgga gagacttttg ttcatgaatt gcaaaagcgg gacattccct
     7501 atctctttgt aaccaacaat acaacccgca ctccagagag tgttaaggag atgttggctc
     7561 agaattttaa tatcgatacg cctctatcga ctgtctacac agcgactttg gcaaccatcg
     7621 actatatgaa tgatttgggg cttgaaaaga ctgtctatgt cgtcggagaa gcaggactca
     7681 aggaagccat caaggcggct ggttatgtgg aagacaagga aaaccctgcc tacgtggtag
     7741 ttggtctgga ttggcaagtc gactatgaaa aatttgccac agcaactctt gctattcaaa
     7801 agggtgccca ttttatcgga accaatcctg acctcaacat cccgacggaa cgtggtcttt
     7861 tgccaggtgc tggctcactg attacgctgc ttgaagtagc gacacgagtg aagccggttt
     7921 atatcggaaa accaaatgcc atcattatgg acaaggcggt tgagcactta ggtttggaac
     7981 gtgaagagtt aatcatggtt ggggacaatt atttgactga tatccgggct gggattgaca
     8041 atggcattcc aacgctcttg gtgacgacag gttttactaa ggcagaagaa gtagctggcc
     8101 tgccaatcgc accgacacat gtggtttcta gccttgcgga gtgggatttt gatgaaaact
     8161 aaattgacct tttggggctc tatgctcttt ctcctctccc tctcaatcct tctgaccatt
     8221 tatctggctt ggattttcta tcctatggag attcagtggc taaacttaac gaatcgagtc
     8281 tatctaaaac cagaaaccat tcaatacaat tttcatatct tgatgaatta tctgaccaat
     8341 ccttttagtc aggtcttaca gatgcctgat tttcgttcgt cagcagctgg tctgcaccat
     8401 ttcgcagtgg tcaagaatct ctttcatttg gttcagctag tagctctagt gacactgcca
     8461 agtttctatg tctttgtcaa taggattgtg aaaaaggact ttttgtctct ttatcgaaaa
     8521 agtctcctgg ctctagtagt cttacctgtg atgattggac ttgggggagt tttgattggt
     8581 tttgaccaat tctttactct tttccatcaa attctctttg tgggagatga tacctggctt
     8641 tttgatccag ccaaggatcc tgttattatg attttgccag agaccttctt tcttcatgcc
     8701 ttcctcctct tttttgccct ctatgaaaac ttctttggct atctgtatct gaaaagtcgt
     8761 aggaagtgaa aggaaagatg t
//
LOCUS       DAVLYR010000075         8775 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLYR010000075 DAVLYR010000000
VERSION     DAVLYR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.075"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            325..3600
                     /gene="rexB"
                     /locus_tag="VYO63_001308"
     CDS             325..3600
                     /gene="rexB"
                     /locus_tag="VYO63_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1672223.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYIDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERKNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3597..7247
                     /gene="addA"
                     /locus_tag="VYO63_001309"
     CDS             3597..7247
                     /gene="addA"
                     /locus_tag="VYO63_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1672224.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFCFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     HVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7507..8685
                     /locus_tag="VYO63_001310"
     CDS             7507..8685
                     /locus_tag="VYO63_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672225.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSIC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDYRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2701 a         1853 c         1870 g         2351 t
ORIGIN      
        1 aaacttcttc tgatatcgaa ttagagtccg atgttgatgg cgataaatca gacaatctac
       61 ccgtcgacat taagatactg ggacaacaca ttgaagtgta ttccgtacct aagaaacaat
      121 cataaaactc aaagccttgt taccgaggct tttttattat gtgattttca gtagccagac
      181 ttattctatc taatacaagt aaagctaagt atttaagatt tagcagatga tgaagaaaag
      241 aaaggatggc ctaaatattt taacagattt acgaggctaa tcatggtata atatagggtg
      301 atgaaaaatt ccaacgaggc tgagatgaaa ttactttata ctgatattcg aacttctttg
      361 acagaaattc taacaagaga ggcagaagag ctagttgcag ctggcaagcg ggtcttctac
      421 attgccccca actctctttc ttttgaaaag gaacgcgccg tgctggaatg cttgtcccag
      481 caggcttctt tttcgattac cgtcacgcgc tttgctcaaa tggctcgtta tctggtcttg
      541 aatgatttac cagctaaaac tactcttgat gatatcggtc ttgggttggc cttttacaaa
      601 tgccttgccg aactcgatcc caaggacttg cgtgtttatg gcgctattaa gcaggatcct
      661 caattgatcc agcagttaat tgagctttac catgagatga ccaaatctca gatgaatttt
      721 ttggacttgg agaatttaac agatgaggat aagagggcgg atttactctt gatttttgag
      781 aaagtaacag cctatcttaa tcaagggcag ttagcccagg gaagtcagtt gtcccatttg
      841 attgaggcta ttgagaatga caaggtaagt agtgatttta atcaaatcgc cttggtcatt
      901 gacggcttta ctcgtttttc tgctgaggaa gagcgggttg tggacttact tcacggcaaa
      961 ggtgctgaga ttgttatcgg ggcttatgct agtaagaaag cctataccag tccttttagc
     1021 gagggcaatc tctaccaagc cagcgtaaaa tttctccatc atctggcttc taaataccaa
     1081 acgcccgctc aggactgttc tcaaactcat gagaagatgg atagttttga caaggcctct
     1141 cgtttgttgg agtcttctta tgacttttca gaactcgctt tggatgtcga tgagaaagac
     1201 cgtgaaaatt tacaaatctg gtcttgtttg acgcaaaagg aggagttgga gctagtagcc
     1261 cgtagcattc gtcagaaatt acatgagaac tcagacctga gctacaagca ttttcgtatt
     1321 ctcttggggg atgtagcttc ttaccagtta tctctcaaaa ccatttttga ccagtatcag
     1381 attccttttt atcttggtag aagcgaagcc atggctcatc atcccttgac tcagtttgtc
     1441 gagtctattt tagctttaaa acgttaccgt tttcgtcagg aggatttgat taatcttctt
     1501 agaactgact tgtatattga cctcagtcag tctgatattg atgcttttga acaatatatc
     1561 cgctatcttg gtattaatgg cttgccagcc tttcagcaaa ccttcaccaa atcccaccat
     1621 ggaaaattta atcttgagcg tttgaatgtc ctccgcctga gaattttagc acctcttgaa
     1681 accctctttg ccagccgaaa acaaaaggct gaaaacctcc tacaaaaatg gagtgtcttt
     1741 ctaaaagaag gagctgtgac caagcagtta caagatttga caaccacttt ggaagctgta
     1801 gaacaggaaa gacaagccga agtttggaag gctttttgcc atgttttaga acaatttgcg
     1861 actgtttttg ctggttcaca ggttagtctg gaagacttcc tagccttgct ccattctgga
     1921 atgagtttgt cccaataccg taccattcca gcaacagtgg acactgttct ggtgcagagt
     1981 tacgatttga ttgcaccatt gactgctgac tttgtctatg ctattggact aactcaggac
     2041 aatttaccaa aaatttctca aaacaccagt cttctgacag atgaagaaag gaaaaaccta
     2101 aaccaaacga ccgaagaagg cgttcaatta ctgattgcca gcagtgaaaa tctcaagaaa
     2161 aatcgctaca ctatgctttc cttggtcaat tctgctcgta agcagttgtt cttgtcggct
     2221 ccaagccttt ttaacgaaag tgaaagtaag gaatctgcct atcttcaaga gttgatccat
     2281 tttggattta ggcggagaga gaagaggatg aatcacaaag gactgtctaa ggaggatatg
     2341 gggtcctatc acagtctttt gtctagtctg gttgcctatc accagcaggg tgagatgagc
     2401 gatactgagc aagatttgac ttttgtcaag gttctgtcgc gtgtcatagg taaaaaacta
     2461 gatcagcaag gtctggaaaa tccagctatc ccaaccagtc caagcagcaa gaccttagcc
     2521 aaggacacct tgcaagctct ctatcctgcc aaacaggagt tttacctgtc tacgtcgggt
     2581 ttgacagagt tttatcgcaa tgaatacagt tatttcctac gctacgtttt aggcttgcag
     2641 gaggaattac gtttgcatcc tgatgcccgt agtcacggga atttcttgca tcgtatcttt
     2701 gaacgcgcct tacagttgcc taatgaagat tcctttgacc aacgtctaga acaagctatt
     2761 caagaaacca gtcaagaacg cgaatttgaa gctatttatc aagaaagttt ggaagcccag
     2821 tttaccaagg aagttttgct tgatgttgca cggacgactg gacatattct ccgacacaat
     2881 ccagccatcg aaaccatcaa agaagaagca aattttggtg gaaaagacca agcctttatt
     2941 caattagaca atggacgcag tgtctttgta cgaggcaagg tggaccggat tgaccgtttg
     3001 aaagctaatg gagcgatagg agtagtagac tacaaatcca gtctgactca gttccagttt
     3061 cctcatttct ttaatgggct caattctcag ttaccaacct atcttgctgc cctaaaaaga
     3121 gaaggggagc agaacttttt cggcgccatg tacttggaaa tggctgaacc tgtccaatct
     3181 ctgatggcgg taaaaagtct ggcaggagca gtggtagaag ccagcaaatc tatgaaatac
     3241 caagggctct tcttggaaaa agaaagcagt tatttaggcg aattttataa caaaaacaag
     3301 gctaatcaac tgacagatga ggaatttcag ctcctactgg actacaatgc ctatctttac
     3361 aagaaagctg ctgagaagat tttagcaggc cggttcgcca tcaatcctta tactgaaaat
     3421 ggcagaagca ttgccccata cgtccagcaa catcaggcta ttacaggctt tgaagccaat
     3481 taccatctgg gccaagcccg tttcctagaa aagttggacc tagctgatgg caagcgtctg
     3541 gtcggagaaa aactcaagca agcttggttt gaaaaaataa gagaggagtt aaatcgatga
     3601 agcttattcc ctttttaagt gaggaggaga ttcaaaaact gcaagaagca gaagcaaatt
     3661 cgagcaagga acagaagaaa actgccgaac aaatcgaagc tatctacact tctggtcaga
     3721 atatcctggt ctcagcatcg gctggttctg gaaagacctt tgtcatggta gagcgcattc
     3781 tggaccaatt ggcgcgtggt gtcgaaattt ctcaactctt tatctcaacc tttaccgtca
     3841 aggctgcaac tgaacttaaa gaacgtttag agaaaaaaat cagcaagaaa atccaagaaa
     3901 cagatgatgt cgatctcaaa caacacttgg gttgccagtt ggcagaccta cccaacgctg
     3961 ccatcggaac catggactct ttcacacaaa aattccttgg caaacatggt tatctgcttg
     4021 atattgcacc taatttccgt attttacaaa accaaagcga gcaacttctt ctcgaaaacg
     4081 aagtctttca tgaggtcttt gaagcgcatt accaaggtaa acagaaagag agctttagtc
     4141 atttgctgaa aaactttgct gggcgtggca aggacgaacg gggtctgcgc cagcaagtct
     4201 ataaaatcta tgacttcctc caatccacca gtaatcctca aaagtggctg agtgaatctt
     4261 tcctcaaagg ctttgagaaa gctgatttta ccagtgaaaa agaaaaactg actgagcaaa
     4321 tcaaacaagc cctctgggat ttggaaagct ttttccgtta ccatctggat aacgatgcca
     4381 aggagtttgc aaaggctgcc tatttagaaa atgttcagtt aattctggat gaaattggct
     4441 ccctaaatca ggagttcgat agtcaggctt atcaggcagt acttgcgcgt gttgtcgcca
     4501 tctctaagga gaaaaacggt cgtgctctga ctaatgccag ccgtaaggct gatttgaagc
     4561 ccctggctga tgcctacaac gaagagagaa agacccagtt tactaaacta ggacaattat
     4621 cagaccagat agcgattctc gactatcaag aacgttatca tggagacact tggaaactag
     4681 ctaaaacctt ccaatctttc atgagcgatt ttgtagaggc ttatcgtcag agaaaacgac
     4741 aggaaaatgc ctttgaattc gctgatatca gccattacac cattgagatt ttagaaaatt
     4801 tcccacaagt tcgtgagtct tatcaggagc gcttccatga agtcatggtc gatgagtatc
     4861 aggataccaa ccatattcaa gaacggatgc tggaattgtt gtctaatggc cacaatcgct
     4921 ttatggtggg agatatcaag caatccatct atcgtttccg tcaggcagac ccgcagattt
     4981 tcaatgagaa attccaacgc tatgcgcaaa atcctcaaga aggcaagctc attctcctca
     5041 aggaaaattt ccgtagtagt tcagaagtgc tgtcagcaac caatgatgtc tttgaacgtc
     5101 tcatggacca agaggtcggc gaaatcaact atgataacat gcaccagctt gtttttgcca
     5161 ataccaaact gactcccaat ccagacaaca aggctgaatt tctcctctac gacaaggacg
     5221 atacaggtga ggaagaagag agtcaaacag aaacgaaact aacaggcgaa atgcgcttag
     5281 ttatcaagga gattctgaaa cttcatcaag aaaaaggtgt tgcctttaag gaaattgccc
     5341 ttctgacctc cagccgcagt cgtaatgacc agattctcct cgccctgtct gagtacggga
     5401 ttcctgtcaa aactgacgga gagcaaaaca attatctcca atccctagaa gtgcaagtca
     5461 tgctagacac tcttcgtgtc attcacaatc ccctgcaaga ctacgccttg gttgccctta
     5521 tgaagtctcc aatgttttgt tttgatgagg atgagctagc acgtttgtcc cttcagaaag
     5581 cagaggataa agtccacgaa aatctctatg agaaactggt caatgcacaa aaaatggcaa
     5641 gtagtcaaaa aggcttgatt cacacagctc tagctgaaaa actaaagcaa ttcatggata
     5701 tcctagcttc ttggcgcttg tatgccaaac cccactctct ctatgacttg atttggaaga
     5761 tttacaacga ccgtttttat tatgactatg ttggggcttt gccgaatggt cctgctaggc
     5821 aggccaatct ctatgcccta gcactgcgtg ctgatcaatt tgaaaagagc aatttcaaag
     5881 gtttgtcgcg ttttattcgt atgattgacc aagtcttaga agcccagcac gatttggcaa
     5941 gcgtggccgt cgcaccgcca aaagatgcag tagagctcat gaccatccac aagagtaaag
     6001 ggctggagtt tccttacgtc tttatcctca atatggatca agatttcaac aagcaagact
     6061 ctatgtcaga agtcattctc agtcgtcaga atggtcttgg tgtcaaatat attgccaaga
     6121 tggagacagg ggcagtagaa gaccactatc ctaaaaccat caaactctcc attcctagtc
     6181 tgacctatag gcagaacgaa gaggaattac agctagcaag ctattctgag cagatgcgtt
     6241 tgctgtatgt tgctatgacg cgggctgaga aaaagctcta tcttgtcggc aagggttctc
     6301 gtgaaaagct ggaatccaag gaatacccag cagccaaaaa tgggaaacta aatagcaata
     6361 ctagactgca agcacggaat ttccaagatt ggctttgggc tatcagtaaa gtgtttacta
     6421 aggacaagct caactttagt tatcgtttta ttggcgaaga tcagttgacc agagaagcta
     6481 tcggagagtt ggaaaccaag agtcctctcc aagatagctc ccaagcagac aatcgtcagt
     6541 cagataccat caaagaagct ctggaaatgc tgaaggaggt ggaagtttat aatactcttc
     6601 accgcgcagc tattgaactt cctagtgttc aaaccccaag tcaaatcaag aaattctacg
     6661 aaccagttat ggatatggaa ggtgtcgaga ttgctggtca aggtcagtca gtaggcaaga
     6721 aaatcagctt cgatttgcca gatttttcaa ccaaagaaaa ggtaactgga gctgagattg
     6781 gtagtgctac tcacgaactc atgcagagaa ttgacctcag ccagcaacta acccttgcta
     6841 gcctaacaga aacactcaaa cacgttcaaa ctagccaagc tgtcagagac aagatcaatc
     6901 ttgataaaat tcttgctttc tttgacacag tactcggtca ggaaattctt gctaataccg
     6961 accatctcta tcgcgagcaa cctttctcca tgctcaaacg agaccaaaag agtcaggaag
     7021 actttgttgt ccgtggtatc cttgatggct atctgcttta cgaaaacaaa attgttctgt
     7081 tcgactacaa gacagaccgc tatgatgaac caagtcaact cgtagaccgc tatcgtggtc
     7141 agttagctct atacgaagag gctttatcac gagcctattc gattgaaaat attgaaaaat
     7201 acttgatttt actcggtaaa gacgaggttc aagttgtaaa agtataactt atactctttg
     7261 aaaatctctt caaaccacgt cagcttcgcc ttgctgtata tatggttact gacttcgtca
     7321 gttctatcca caacctcaaa acagtgtttt gagctgactt cgtcagttct atccacaacc
     7381 tcaaaacagt gttttgagct gacttcgtca gttctatcta caacctcaaa acagtgtttt
     7441 gagctgactg cggctagctt tctagtttgc tctttgattt ttattgagta ttagaaagga
     7501 gacctcatga cacttccagt tagaaaatcc ctgcacgatg cagttttaca ggcttcgaaa
     7561 gcggatactt gggaacaagc taccaaggaa tggaatgaag tttccttgat ttttaacggt
     7621 atcggccgta gcatttgtgt ctgtggaaat gtcatcaaat acgcttatga actctttaac
     7681 ggtgtcacag gccaacgcct ctttccaatt ggtagcgact gtgttcgtca ttttcatcga
     7741 ttgtcccttg accagcaatt ggaagaggaa gaaaaactgc tcagaaaggt tgaaaatcta
     7801 accagaaaag ctcagaaaaa ggaaaaaatc aaggtcaata aaagtgactt tgacgagcga
     7861 cttctaaaat ggctctggga aaaaggtgtt ttcaaaccca atcgtggcaa tcaatttgcg
     7921 cctgagagag actaccagct tttcctagaa gtctttcagg gaggaagttg gaccaaggcg
     7981 gaaccaaaga aaaaggctcg gatggaagaa gtccttgaaa agtgtatcaa acccttttta
     8041 cttgggaaat cggatgacca actctacctt gtcaagctag gcaaggagaa aattgactac
     8101 gaacaagaac tccgtatcca ggcagagaaa gaacgcaaga agagggataa aatcgccaag
     8161 caatatgcag acaatctggt tctagccatg ggacccgcag agcgtgctta tcaagattac
     8221 tttggcttta cagagaccct gactcaagaa gaacgcaagt gggaaaaaat tctttttggt
     8281 aaaaatagag cagaacgggc tattaaggcc aaacaatacc aaaaagagct ggaaaaagac
     8341 caacgaattg ccagtcagga tccaattgaa agaaaacaga aacagacttg gctcctcaat
     8401 tcctattttc gtgagcttcc tgaagaaaaa gctcgatttt ctaggctctt attagaatat
     8461 cgaaaaaatg gtgaagtacc cttttcaact gaatacctgt cagatcatct catcgacttt
     8521 ttctataaaa tgaaagcctt tgaatttgaa atcgcaccag aacaagtccg agattttcta
     8581 aaaaaaagcc tgcaggaaga ctatcgctca tcagcacaag gaagctggat agaaggaatt
     8641 ctcctcaact gcctcaaacc atttttagaa cgattagtga tatgagtaac acctaccgtg
     8701 taaatttcat ggtaggtgtt attgtacccc caaataatat aaaacaaagt atgaaagcgc
     8761 atctcaattt taaga
//
LOCUS       DAVLYR010000076         8775 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLYR010000076 DAVLYR010000000
VERSION     DAVLYR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.076"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            7..5256
                     /locus_tag="VYO63_001311"
     CDS             7..5256
                     /locus_tag="VYO63_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1672226.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVIGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGEPEVQPTLPEAVVTDKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQIVT
                     SYEELHGKKISEPVETVTILKEMKPKILVKGTKENPKEKTVPVLTLTKVTEDAMNRSA
                     NLNYELDNKDNAEISSIIAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMI
                     YDRGQGSETSKLDEKTLRLELKKVEIKNISSTNLVKVNDDGTEIPSDFMSEKPSDEDV
                     KKMYLKITSRDNKVTRLAVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEYIHY
                     IEKPVPKVNNVYYNFNELVRDMQEHPNDEFKLGADLNATNVSAFGKSYVTKDFKGKLL
                     SDGDNHYTIHNLSRPLFGNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTTIEN
                     VKVKGNIVGKDWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVE
                     RAYVNANLIGKKAKAAGIAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESGGVV
                     GSMKHHGSVEDSVSMMKVPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGHSSY
                     QRSKDKNRIRPISEEEAKSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQDYK
                     VDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDI
                     DHVMIHYADKTKEIKAVHQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKDIKD
                     RLATVELISPEVRALMDKRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDKLVK
                     SLIENADHQLNSDEAAMKALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLMMFK
                     PDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFT
                     NDTDLNDWFIHAAKNVYIVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAH
                     MFLISTYNTMAYSSFEKYGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSVRDQ
                     LLKSQNRVPTPVWDNQNYHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWYMGA
                     MASIFQDPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAY
                     AQGMLQTPDKSGNGEYGALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNYNDA
                     LMMLDHLEADAVIPKLHGNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIVINN
                     IDDLVNNNLMTKHGAPSDRTYNPEDFDSAYVNINIMTGIYGGNTSQGAPGAASFKHNT
                     FRMWGYFGYENGFISYASSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKKQYF
                     KDVKSKAEKGFTAIEIDGRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTVDLK
                     SKVFKALLKNTDGFFNKLFKEDI"
     gene            5502..6353
                     /gene="ylqF"
                     /locus_tag="VYO63_001312"
     CDS             5502..6353
                     /gene="ylqF"
                     /locus_tag="VYO63_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1672227.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWCQYFESQEIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6340..7119
                     /locus_tag="VYO63_001313"
     CDS             6340..7119
                     /locus_tag="VYO63_001313"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1672228.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKEN"
     gene            7135..8685
                     /locus_tag="VYO63_001314"
     CDS             7135..8685
                     /locus_tag="VYO63_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1672229.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         2919 a         1487 c         1806 g         2563 t
ORIGIN      
        1 ttagaattgt cattattaaa aaaagataaa ttttccattc ggaaaataaa gggtattgtt
       61 ggttcggtat tcttggggag tcttttattt gcaccgtcag ttattggtgc atcgacttat
      121 cattacttag attatagtag tttgacacaa actgaacgtg atcaactcaa acaaggtaga
      181 cctgatgaat ccaaagagtc atatgctttg gattatgaaa aagacgcact accaaacaca
      241 ggcagttctc aatctattat gactgcattg ggtttgttag ctatcgggag tcttattgtg
      301 attatcacaa aagataatag aaataaaaaa atagctacat ttttgattgt aggggcgaca
      361 ggtttagtta cactatcaac tgcttcagca cttaatttga atgctaacat ccatgagtct
      421 ggacgtgatg gtgtgttgca aatttctggt tacagatatg ttggttactt ggagcttgat
      481 gataaaactg tttcgtcggt ttctccagca tcaactgttt cgccagttga gcaacccaaa
      541 gttgtaacag agaaagggga accggaggtt caaccagcct tacccgaagc tgttgtgact
      601 gacaaaggcg aaccggaagt acaaccgaca ttaccagaag ctgttgtgac tgacaaaggc
      661 gaaccagaag ttcatgagaa gcctgactat acgcaaccaa taggtgcaaa ccttgttgag
      721 cctgaagttc atgagaaact agcatacact gaaccagtag gtacaacagg agtggatgaa
      781 aatggaaact tgattgaacc acctgttaac gacattccag agtatactga accaatatct
      841 acagtttcag aagttgcatc agaaagagaa gagttgcctt ctttacatac cgatattcgt
      901 acagagacta ttcctaaaac cactatagaa gaatctgatc ctagcaaatt tataggcgat
      961 gattctgtta aagaagttgg tgaggatggc gaacgtcaaa ttgttactag ctatgaagag
     1021 ttacatggca aaaaaattag tgaaccagtt gaaacggtaa caattttgaa agaaatgaaa
     1081 cctaaaattc ttgtaaaagg tacaaaagaa aatccaaaag aaaaaacagt tcccgttttg
     1141 accttgacca aagtaacaga agatgctatg aatcgttctg ccaacttaaa ttatgagctt
     1201 gataataaag ataatgctga aatatcaagt attatagctg aaattaaaga tggtgataca
     1261 gtagttaaaa aggttgattt atctaaagaa aaattaactg acgctgtaca aaatttggat
     1321 ttgtttaaag attataagat tgcaacaaca atgatttatg atcgtggtca aggttctgaa
     1381 acgtctaaac tggatgaaaa aactttgcgt ttggagttga aaaaggtcga aattaaaaat
     1441 atttcatcta ctaatttagt gaaggtaaat gatgatggta ctgaaatacc tagtgatttc
     1501 atgagcgaaa aaccttctga tgaagatgtg aagaaaatgt acttaaaaat cactagtcgt
     1561 gataataaag taactcgttt agcagttgat aagattgagt tagtaactga aaaagaaaaa
     1621 gaactttata aaattacagc aagcgctcaa gatttaattc aacatgtcga tccatctaag
     1681 actagaaatg aatacattca ttacattgaa aaaccagttc caaaagtcaa taatgtatat
     1741 tacaatttca atgaacttgt gagagatatg caagaacatc caaatgacga gtttaaattg
     1801 ggtgctgacc ttaatgccac aaatgtatca gcatttggta aatcatatgt tacgaaagat
     1861 ttcaaaggta agctcttgag tgatggtgat aatcactata ccattcataa cttgtcacgt
     1921 ccgttatttg gcaatgttat tggtggaacg attaagaata ttaatcttgg taatgttgac
     1981 ataaatatgc catgggcgaa ccaagttgca gctgtagcga atattattaa aggtggaact
     2041 accattgaaa atgttaaggt taaaggtaat atagtaggta aagattgggt atctggattt
     2101 attgataaga ttgataacca aggtacactc agaaatgtgg cctttatcgg taacgtaact
     2161 tctgtcggcg atggtggaca attcttgact ggtatcgttg gtgagaactg gaaaggactc
     2221 gtcgaacgcg cgtatgttaa cgcaaatctt attggcaaga aagccaaggc tgcgggcatt
     2281 gcttattgga cacaaaatga aggaaataac aatactgttc gtcaggaagg agcaatcaaa
     2341 aaatctattg ctaagggaac aattcaagta actgaggcga ttgaaagtgg tggtgttgta
     2401 ggaagcatga agcaccacgg ttctgtcgaa gattcagttt ccatgatgaa ggtacctaat
     2461 ggtgaaattt tctatggttc tagtgatatt gattatgatg atggctactg gacaggagat
     2521 aatgtaagac gaaattatgt tgtaataggt gttagtgatg ggcattctag ctaccagcgt
     2581 tcaaaagata aaaatcgtat cagacctatt tctgaggaag aggcaaaatc taaaattgaa
     2641 gctacaggaa ttaccgcaga taaatacgaa atcaatgaac ctgtggtcaa tcgcttaaat
     2701 cgtttaacca gaagagaaga tgaatataag tcaacccaag actataaagt agatcgagat
     2761 ctggcttatc gtaatattga gaaactgcaa ccattttata acaaagaatg gattgtcaat
     2821 caaggtaaca aacttgcaga agattcaaac ttagctaaga aagaagtcct ttctgttacg
     2881 ggtatgaagg atggtcaatt cgtgaccgat ttatcagata ttgatcatgt gatgattcac
     2941 tatgcagata agactaaaga aatcaaagct gttcatcaaa aagagtctaa agtagcgcaa
     3001 gttcgtgagt atagtatcga tggattagat gacattgttt atacaccaaa catggttgat
     3061 aaaaatcgtg atcaattgat taaagacatt aaagataggt tagcaactgt tgaattaatt
     3121 tctcccgaag tccgtgcttt gatggataaa cgagatacat ctagagatcc taatgctaat
     3181 tcggatgagc gtaagaatgg ttatatccga gacctttatt tcgaagaaag tttttctgaa
     3241 acgaaagcta atcttgataa attggtcaaa tctttgattg aaaatgctga ccatcaatta
     3301 aatagtgacg aagcagctat gaaagcactt gttaagaagg tggatgaaaa taaagctaaa
     3361 atcgtgatgg ctcttactta ccttaatcgt tactatgata taaagtatgg ggatatgact
     3421 atcaaaaact tgatgatgtt taaacctgat ttctatggta agtcagttga ccttcttgac
     3481 ttcttgattc gaattggatc aagtgagcga aatatcaagg gtgacagaac tttggatgct
     3541 tatcgtgata tgattggtgg aactattggt aagtctgaat tacatggctt cttagattac
     3601 aacatgcgtc tcttcactaa cgatactgat ttaaatgatt ggtttatcca tgcggctaag
     3661 aacgtataca ttgtggagcc taaaacaacg aacccagatt ttgttaataa acgacatcgt
     3721 gcatttgatg gtttgaataa tggtgttcat aatcgtatga tcctacctct attaactctt
     3781 aagaatgcac atatgttctt gatttcgact tacaatacaa tggcttatag ctcttttgaa
     3841 aaatatggta agtatacaga agctgaacga gaagcattta aagataaaat taaagaagtt
     3901 gctcacgcac aacaaactta tcttgacttc tggtcgcgtt tagcattgcc aagcgtacgt
     3961 gaccaattac tcaagagtca aaatagggtt ccaacacctg tttgggataa ccaaaactat
     4021 cataacgtcg aaggtgtaaa tcgtatgggt tatgataaga ataataaacc aattgcacct
     4081 attcgtgaat tatatggacc tacttggcgt tatcatacta caaattggta catgggagct
     4141 atggcatcta ttttccaaga tcccaataat aatgaccaag tttatttcat gggaacaaat
     4201 atgattagtc cgtttggtat ttcagccttt actcacgaaa caacacacgt aaacgataga
     4261 atgctttact tcggtgggca tagacaccgt caaggtacag acgttgaagc ctatgcgcaa
     4321 ggtatgttgc agacacctga taaatctggt aacggtgaat atggtgctct tggtcttaac
     4381 atggcttatc atcgtgaaaa tgacggtgac caatggtata attatgatcc agataaattg
     4441 aaaactcgtg aagatatcga ccgttatatg agaaattata atgatgctct tatgatgtta
     4501 gaccatcttg aggctgatgc tgttatccct aaattacatg gtaatattag tcgatggttc
     4561 aagaaaatgg atagacaata tcgcaaaaat ggagagctac atcaatttga taaagttcgt
     4621 gaattaactg aagatgagaa gaagaaaatt gttatcaaca acattgatga tttagtcaac
     4681 aataatttga tgacaaaaca tggcgctcct agtgacagaa catataatcc agaagatttt
     4741 gatagtgctt acgtaaatat taatataatg accggtatct atggtggtaa tactagccaa
     4801 ggagcacctg gagctgcatc attcaagcat aatactttcc gtatgtgggg ctattttggt
     4861 tatgaaaatg gattcattag ttatgcatct agcaaatatc aaggcgaagc tgacaaaacc
     4921 aataaaaaat tattgggtga tgatttcatt atcaagaaag tatctaagga taaattcaat
     4981 aatcttgaag agtggaagaa acaatacttc aaagatgtta agtcaaaagc agaaaaagga
     5041 tttactgcta ttgaaattga tggtcgtcaa attacaaatt atgctcagtt gaaaacttta
     5101 tttgctgaag ctgttcaaaa agatattgat ggtatgtcag atccgaaaat taaagatcat
     5161 ttcaaaaata cagtagactt gaaatccaaa gtattcaaag cccttcttaa aaacacagac
     5221 ggtttcttta ataaactgtt taaagaagac atttaaatgt ttaaaatata aagaggacag
     5281 ttttttgctc cctctgaaaa gtcatcattt gatggctttt ttctatatag ggtaaaagat
     5341 agggtaaaag gctatcatcg gacaaaataa agaaggcatg atataatata aagtagattt
     5401 ttatatcata aaacaagaac tgtttggaca tcattcattt ttagattgag atctcttagt
     5461 tctagattaa cagcttcaga gctacgaaag gaaaaaactc aatggctact attcaatggt
     5521 ttcctggtca catgtctaaa gcgcgtcgac aggtgcagga aaatttaaaa tttgttgatt
     5581 ttgtgacgat tttagtagat gcacgcttgc ctctatctag tcaaaatcct atgttgacca
     5641 agattgttgg tgataaacca aaactcttga ttttaaacaa ggccgacttg gctgatccag
     5701 caatgaccaa ggaatggtgt cagtattttg aatcacaaga aatccagacg ctagctatca
     5761 actccaaaga gcaagtgact gtaaaagttg taacagatgc ggccaagaag ctcatggctg
     5821 ataagattgc tcgccagaaa gaacgtggga ttcagattga aaccttgcgt accatgatta
     5881 tcgggattcc aaacgctggt aaatccactc tgatgaaccg tttggctggt aaaaagattg
     5941 ctgttgttgg aaacaagcca ggggtcacaa aaggtcaaca atggcttaaa accaataaag
     6001 atctggaaat cttggataca ccggggattc tctggcctaa gtttgaggat gaaactgttg
     6061 cacttaagtt ggcattgact ggagctatca aggatcagtt gcttcctatg gatgaggtta
     6121 ccatttttgg tatcaattat ttcaaagaac attatccaga aaagctggct gaacgcttca
     6181 aacaaatgaa aattgaagaa gaagcgcctg tgattattat ggatatgacc cgcgccctcg
     6241 gtttccgtga tgactatgac cgtttttaca gtctcttcgt gaaggaagtc cgtgatggca
     6301 aactcggtaa ctatacctta gatacattgg aagacctcga tggcaacgat taaagaaatc
     6361 aaagaactcc ttgtgacagt caaggagtta gaaagcccta tttttttaga gcttgaaaag
     6421 gataatcgct caggagttca aaaggaaatc agcaagcgta aaagagccat tcaagctgaa
     6481 ttagatgaaa atttgcgctt ggaatccatg ctttcttatg aaaaagaact ttataagcaa
     6541 ggattgacct taattgtagg tattgatgag gttggtcgtg gtcctcttgc tggtcctgta
     6601 gtcgctgcgg ccgtcatttt acctaaaaat tgtaagatta aaggtctcaa cgacagcaag
     6661 aaaattccta aaaagaaaca tctggagatt ttccaagccg ttcaagacca agccttgtcg
     6721 attggaattg gtatcataga taatcaggtc atcgaccaag tcaacatcta tgaagcaacc
     6781 aaactagcca tgcaagaagc aatctcccag ctcagccctc aaccagagca ccttttgatt
     6841 gatgccatga aactggactt gcccatttca caaacctcca ttatcaaagg agatgccaac
     6901 tccctctcta tcgcagcagc atctatagta gccaaggtaa cacgtgatga attgatgaag
     6961 gaatacgatc agcagttccc tggctatgat ttcgctacta atgcaggata tggcacagct
     7021 aaacatctgg aaggcctcac aaaactagga gttaccccaa ttcaccgaac cagctttgaa
     7081 cccgttaaat cactggtttt aggtaaaaaa gaaaattaat tgaaaggaaa taccatggag
     7141 gaacagtcgg aaatagtccg ttctaagaaa gaattcgcct ttgcatccag cactatacta
     7201 tcccaagttg gtcgaggaat cattgtcggc ctcatcgttg gaattatcgt cggatccttt
     7261 cgtttcttaa ttgaaaaggg cttccacctg atacaaggag tttatcaaga tcaagggtac
     7321 ttagtgcgca atctttttgt actggttttg ttttatatac tcatctgttg gctcagtgcc
     7381 aaactaacac ggtcagaaaa agatattaaa ggctcaggaa ttcctcaagt cgaagccgaa
     7441 ctgaaaggcc tcatgtccct caactggtgg ggcattcttt ggaaaaaata tgtgctaggt
     7501 attcttgcta ttgccagtgg actcatgctg ggtcgagagg gacccagcat tcaacttgga
     7561 gcagttggtg gtaaaggaat tgccaagtgg ctcaaatcca gtccagtaga ggaacgttcc
     7621 ttgattgcca gtggagctgc agcaggttta gccgcagcct ttaatgctcc tattgcagca
     7681 cttctctttg ttgtagaaga agtctatcac catttttcgc gctttttctg ggtctcaact
     7741 ctagcagcca gcatcgtagc aaactttgtg tctctactca tgttcggttt gacaccagta
     7801 ttggatatgc cagataacat tcctcccatg accctagatc agtattggat atatctcgtc
     7861 atgggaattt tccttggatt ttcaggtttt ctctatgaga aagctgtatt aaacgttgga
     7921 agagtttatg acttgattgg tcaaaaaatc catttggata gggcttatta tcccatcttg
     7981 gcttttatcc ttatcatacc agtcggaatc ttcttacctc aaatcattgg tggcggaaat
     8041 cagcttgtcc tttctttaac tgaacaaaat tttagtttcc aagttttatt agcttacttt
     8101 ttaatccgct ttatttggag tatgattagc tatggaagtg gactgccagg aggaattttc
     8161 ctccccattt tagctcttgg ttctttgctt ggtgccttag ttggtgttat ctgtgtcaat
     8221 cttggacttg tcagtcaaga gcaattccct atatttgtca ttctaggaat gagtggctat
     8281 tttggagcca tatcaaaagc tcccttaact gctatgatcc tcgtaactga gatggtagga
     8341 gatattcgca acctcatgcc acttggtctt gtcactcttg tttcttatat tatcatggat
     8401 ttgctcaaag gtacgccagt ctatgaagcc atgctggaaa aaatgcttcc agaagaagta
     8461 tctagcgaag gagaagttac acttatcgaa ataccagttt ctgataaaat tgctgggaaa
     8521 caagttcatg aactcaactt accacacaac gtcctcatca caactcaagt ccataatggc
     8581 aagagccaaa cagttaacgg ctcaaccaga atgtatctgg gtgatatgat tcacctggtt
     8641 attccaaaaa gtgaaattgg aaaagtcaaa gatttgttgt tgtagtacga tcatttacat
     8701 aatttatgtt atgtaaatga tcggcttgat ttattcggct tgatttattt agaaaaccga
     8761 ttctcagaaa tgaga
//
LOCUS       DAVLYR010000077         8774 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLYR010000077 DAVLYR010000000
VERSION     DAVLYR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.077"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            105..992
                     /locus_tag="VYO63_001315"
     CDS             105..992
                     /locus_tag="VYO63_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEV1672230.1"
                     /translation="MLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIAST
                     LYFYLSPISYASSEIIARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATAL
                     MPPLCTVGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDS
                     KMRRILLLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHT
                     LKLTISGNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKD
                     KEAKEKEKANKFYTESEEQ"
     gene            1159..3246
                     /gene="topA"
                     /locus_tag="VYO63_001316"
     CDS             1159..3246
                     /gene="topA"
                     /locus_tag="VYO63_001316"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1672231.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEENIK"
     gene            3352..3711
                     /locus_tag="VYO63_001317"
     CDS             3352..3711
                     /locus_tag="VYO63_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1672232.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            3804..4436
                     /locus_tag="VYO63_001318"
     CDS             3804..4436
                     /locus_tag="VYO63_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1672233.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGVLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQVGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            4480..5136
                     /locus_tag="VYO63_001319"
     CDS             4480..5136
                     /locus_tag="VYO63_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1672234.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5155..5775
                     /locus_tag="VYO63_001320"
                     /pseudo
     CDS             5155..5775
                     /locus_tag="VYO63_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            5803..6237
                     /locus_tag="VYO63_001321"
     CDS             5803..6237
                     /locus_tag="VYO63_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV1672235.1"
                     /translation="MVAGSMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6249..7286
                     /gene="leuB"
                     /locus_tag="VYO63_001322"
     CDS             6249..7286
                     /gene="leuB"
                     /locus_tag="VYO63_001322"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1672236.1"
                     /translation="MAKKIVALVGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLATIGSPQYDGAVVRPEQGLMTLRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSV
                     PDIAGQGIANPISMILSVSMMLRDSFGGYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7283..7552
                     /locus_tag="VYO63_001323"
     CDS             7283..7552
                     /locus_tag="VYO63_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1672237.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            7556..7915
                     /gene="leuD"
                     /locus_tag="VYO63_001324"
     CDS             7556..7915
                     /gene="leuD"
                     /locus_tag="VYO63_001324"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1672238.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8134..8277
                     /locus_tag="VYO63_001325"
     CDS             8134..8277
                     /locus_tag="VYO63_001325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672239.1"
                     /translation="MTSDKAGLERKFAAKEGKRNKPGVVLCGSMDELCALAQLNPKIE
                     AFY"
     gene            8296..8550
                     /locus_tag="VYO63_001326"
     CDS             8296..8550
                     /locus_tag="VYO63_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672240.1"
                     /translation="MLLGCILPWKPEAFEKLKAYGDGREELMTDVRGTSCFVIKFGKA
                     GEQLAAKLWEEGKMVYASYASMTKRLKLAMRARCNGVYGR"
     gene            8540..8701
                     /locus_tag="VYO63_001327"
     CDS             8540..8701
                     /locus_tag="VYO63_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672241.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNL
                     WDYRQVEYY"
BASE COUNT         2680 a         1580 c         1962 g         2552 t
ORIGIN      
        1 aagaattaga actttccaga agtgatttag cgattttact atgtgccatg cttatcgcct
       61 ctatcggatt aaatatggat tcgactcccg tgattattgg agccatgtta atctctcctt
      121 tgatgacacc tattctggga gtggggctct ctctagctat atttgatttt aaattgttaa
      181 gaaaatcttt taaaatatta gctattcaaa ttcttgccag tctaatagct tcaacacttt
      241 atttttatct ttctcccatt tcgtatgcta gttcggagat tattgctaga acctctccga
      301 ctatttggga tgttctcatt gcttttgtag gagggatagc aggtattatt ggtgctagga
      361 aaaaagagac caataatatt gttcctggtg ttgctattgc aaccgccttg atgcctcctc
      421 tttgtacagt aggttatgct attgcttctg ctaatctaaa atttatcata ggctcctctt
      481 acctattcct catcaattgt agctttattg tcattgcgac ttatataggt gttaggttga
      541 tgatggttaa gaaacattat tttaaagata atgaagaaga ctctaaaatg cgtaggattt
      601 tgcttctagt tgctgttttg ctgatgattc cgagtttcat ctctgcaacg actttagtga
      661 gagaaacgtt gaaaaaagag tcccttaaga aatttatatc agagcagttt caggggcata
      721 atattttgaa aaaaacctat tctaaaaaga ctcataccct aaagctaacc atttcaggaa
      781 attatttgac agaagaagaa ctcgatatga tttcaagtaa gagaggtgac tatggtttaa
      841 gtgatgtttc tgttcaagtt tcacaattgt ctgattcaga acaacttagt aaggaagaac
      901 tggtggagta tttcttccag tatatcaagg ataaggaagc aaaagaaaag gaaaaagcta
      961 ataagtttta tacagagtct gaggagcaat aatttcttga gaatagctgg tttttctcgt
     1021 gagtcttcta tgtatatcaa aggaagactg aggttttaag tataaaactt ttcttctatt
     1081 atagtagcta agtcttgaca gggttcaaaa aatggtttac actttataaa gtttattact
     1141 ttgaaaaggt gtgatactgt ggctacggca acaaaaaaga aaaaatcaac agttaaaaaa
     1201 aatctagtca tcgtggagtc gcctgctaag gccaagacga ttgaaaaata tctaggcaga
     1261 aactacaagg ttttagccag tgtcgggcat atccgtgatt tgaagaaatc cagtatgtcc
     1321 gttgatattg aaaataatta tgaaccgcaa tatatcaata tccgaggaaa aggccctctt
     1381 atcaatgact tgaaaaaaga agctaaaaaa gctaataaag tttttctcgc gagtgacccg
     1441 gaccgtgaag gagaagcgat ttcttggcat ttggcccata ttctcaactt ggatgaaaat
     1501 gatgccaacc gtgtggtctt caatgaaatc accaaggatg cagtcaaaaa tgcttttaaa
     1561 gaacctcgta agatcgatat ggacttggtc gatgcccaac aagctcgtcg gatcttggat
     1621 cgcttggtag ggtattcgat ttcgcctatt ttgtggaaga aggtcaagaa gggcttgtca
     1681 gcaggtcgcg ttcagtccat tgcccttaaa ctcatcattg accgtgaaaa tgaaatcaat
     1741 gccttccagc cagaagaata ctggacagtt gatgctgtct ttaaaaaggg aaccaaacaa
     1801 tttcatgctt ccttctatgg agtagatggt aaaaagatga aactgaccag caatgacgaa
     1861 gtcaaggaag tcttgtctcg tctgactagt aaagactttt cagtagatca ggtggataag
     1921 aaagagcgca agcgcaatgc tcctttaccc tataccactt catctatgca gatggatgct
     1981 gccaataaaa tcaatttccg tactcgaaaa accatgatgg ttgcccaaca gctctatgaa
     2041 ggaattaata tcggttctgg tgttcaaggt ttgattacct atatgcgtac cgattcgact
     2101 cgtatcagtc ctgtagcgca aaatgaggcg gcaagcttca ttacggatcg ttttggtagt
     2161 aagtattcta agcacggtag caaggtcaaa aacgcatcag gtgctcagga tgcccatgag
     2221 gctattcgtc cgtcaagtgt ctttaataca ccagaaagca tcgctaagta tctggacaag
     2281 gatcagctca agctatatac ccttatctgg aatcgttttg tggctagcca gatgacagcg
     2341 gccgtttttg ataccatggc tgttaaattg tctcaaaaag gggttcaatt tgctgccaat
     2401 ggtagtcagg ttaagtttga tggttatctt gccatttata atgattctga caagaataag
     2461 atgttaccgg acatggttgt tggagatgtg gtcaaacagg tcaatagcaa accagagcaa
     2521 catttcaccc aaccgcctgc ccgttattct gaagcaacac tgattaaaac cttagaggaa
     2581 aatggggttg gacgtccatc aacctacgcg ccaaccattg aaaccattca gaaacgttat
     2641 tatgttcgcc tggcagccaa acgttttgaa ccgacagagt tgggagaaat tgtcaataag
     2701 ctcatcgttg aatatttccc agatatcgta aacgtgacct tcacagctga aatggaaggt
     2761 aaactggatg atgtcgaagt tggaaaagag cagtggcgac gggtcattga tgccttttac
     2821 aaaccattct ctaaagaagt tgccaaggct gaagaagaaa tggaaaaaat ccagattaag
     2881 gatgaaccag ctggatttga ctgtgaagtg tgtggcagtc caatggtcat taaacttggt
     2941 cgttttggta aattctacgc ttgtagcaat ttcccagatt gccgtcatac ccaagcaatc
     3001 gtgaaagaga ttggtgttga gtgtccaagc tgtcatcagg gacaaattat tgagcgaaaa
     3061 accaagcgta atcgcctctt ctatggttgc aatcgctatc cagaatgtga atttacctct
     3121 tgggacaagc ctgttggtcg tgactgtcca aaatgtggca acttcctcat ggagaaaaaa
     3181 gtccgtggtg gtggcaagca ggttgtttgt agcaaaggcg actacgagga agaaaatatt
     3241 aaataagagg aggggcccga aagtaaattt caggctcctt ttggttagaa cttgacaaaa
     3301 tacatcattt tatgcgaaac tagaagaaga ttattttaac taggagaagt tatgcgtctt
     3361 atctatctga taattggttt tttatcactg gccttggcta ttgttggggt tgttttaccc
     3421 ttgttgccga caacgccttt ccttttgttg tctattgctt gtttctccag aagttccaag
     3481 cgtttcgaag actggcttta tcataccaag ctctatcaaa catatgtagc tgattttcgc
     3541 gagactaagt ctattacgcg tgaacgaaag aaaaaaatca tcgtctctat ctacgtcttg
     3601 atgggaattt ctatttattt tgcacctctt ttaccagtca aaatcggtct gggtgctttg
     3661 accatcttta tcacctatta tctcttcaag gtcattccag acaaagaata attaaaacag
     3721 tagttatttc ccttgataaa attgaaagca tattcataac aatatgatat aataaatttg
     3781 aagtaatatt caaggagaat caaatgattt acgaattttg tgctgaaaat gtgaccttgc
     3841 ttgaaaaagc gatgcaggct ggagctcgtc ggattgaact ctgtgataat ctagcagttg
     3901 gtgggacaac ccccagctat ggagtgacta aggcagcggt tgaactggca gctaactacg
     3961 atacaaccat catgaccatg attcggccac gtggtggtga ctttgtctat aatgacctag
     4021 aaattgctat catgctagaa gacattcgtt tgactgctca ggctggaagt caaggggttg
     4081 tatttggagt tttaactgct gataaaaagt tggataagcc taatctggaa aagttaattg
     4141 ctgcatcaaa aggaatggaa attgtctttc acatggcctt tgatgaacta agtgatgaag
     4201 atcaaccgga agctattgac tggctcagtc aagtcggtgt cactcgtatc ctaactcgtg
     4261 ctggtgtgtc tggcgactcc ttagaaaaac gttttgttca ctatcacaga attttggagt
     4321 acgctaaagg taaaattgaa attctaccag gtggggggat tgaccttgac aaccgtcaaa
     4381 cctttatcga ccaggtgggg gtaacacagt tgcatggtac taaggttgtt ttttaaaaaa
     4441 tagaaaggaa ctgctagctt tgggtagcag ttttcactta tgtttgaaat ttttaaatcc
     4501 tatcaattta atcaagaaaa ggctcatgat tatggtttta tagaaaatag cgaagtctgg
     4561 acatatagtt gccagatttt gcaaggtgac tttgtcatga ctgtgtccat cactgctgat
     4621 aatgtgaact ttcaagtctt tgaccaagag actggcgacc tctatcctca cgtttatatg
     4681 gaaagcatga ggggaagttt tgtcggaaat gtccgtgagg cttgtctgga gattctttac
     4741 cagattcgga aggcttgttt tgatgtgcaa gattttatct gtcatcagac taagcgtatc
     4801 atgactcaag ttcaggaaaa gtatggaaac cagttggagt atctgtggga aaaatcgcct
     4861 gatacagctg tattgcgcca tgaaggcaat caaaagtggt atgccgtctt gatgaaaatc
     4921 tcttggaata agctggaaaa gggcagagaa ggacaagtgg aagcagtcaa cctcaagcat
     4981 gaccaagtag ctaatttact ttcacaaaag gggatttatc cagcctttca tatgagcaag
     5041 cgctactgga ttagtgtgtc ccttgatgat actttatcag atgaagaagt actggaattg
     5101 atagaaaaaa gttggaactt aacctctaaa aaatgaaata ttttaataat tttcatgaac
     5161 tttcaattag ctaaatattc tttactgaag aaattttcag aaaatatagg atttaccaca
     5221 ctagaggaat atggtgcatc ttcaaatacc tgattgagaa tgtcaagacg gatcgtcaga
     5281 tcatctattc gcctcactgt catgatgacc tcggaatggc agtggcaaat agccttgctg
     5341 ctgtcaagaa tggtgcagga cgtgttgaag ggactatcaa tggtattagg gagcgagctg
     5401 gaaatgctgc tttggaagaa attgcagtgg ctctcaatat tcgccaagat tactaccaag
     5461 tagaaaccag tattgtccta aatgagacca tcaatacgtc agaaatggtt tctcgcttct
     5521 ctggtattcc agttcctaaa aacaaagccg tcgttggtgg caataccttc tcccacgaat
     5581 ctggtattca ccaagatgga gtccttaaaa atcctctcac ttatgagatc atcacacctg
     5641 aattggttgg tgttaagatt ccgcttgggt cgccatgctt ttgttgagaa actgagagaa
     5701 ttggccctag attttacaga agaggatatc aaaccactct ttgctaagtt caaggcactg
     5761 gccgataaga agtaagaaat cacagatgca gatattcgag ctttggtagc tggaagcatg
     5821 gttgaaaatc cagaaggctt ccactttgat gatttacaac ttcaaactca tgcagataat
     5881 gacattgaag cgctcgttag cctagccaat atggatggtg agaaagtcga atttaatgcg
     5941 acagggcaag gttccgttga agcaatcttt aatgctatcg ataagttctt taaccaatct
     6001 gttcgtttgg tgtcctacac tatcgatgcg gtaacagatg gaatcgatac ccaggatcgg
     6061 gttttggtca ctgttgaaaa cagagataca gaaaccatct ttaatgcagc agggcttgat
     6121 tttgatgtgt tgaaggcttc tgctattgtc tatataaacg ctaatacctt tgttcaaaaa
     6181 gagaatgcag gtgagatggg acgcagtgtt tcttaccacg atatgcctag tgtgtaaagg
     6241 agaaggctat ggcaaagaaa atagtagctc tagtaggaga cggaattggc ccagaaatca
     6301 tggaggttgg tttagaagtt ctggaggctc tagctgaaaa aacaggtttt gactatgaga
     6361 ttgacagacg accgttcgga ggtgcagata ttgatgcagc atggcctccc ttacctgatg
     6421 aaacccttaa ggcaagtagg gaagcagatg ctatcctact agcaactatc ggtagtcctc
     6481 agtatgatgg agcagtggtt cgccctgaac aaggcctgat gactctccgt aaggaactta
     6541 atctttacgc taatattcgt cctgtaaaaa tctttgacag tctcaagcat ttgtcaccac
     6601 tcaaactgga acgaattgct ggtgtagact ttgtcgtggt gcgtgaattg acaggcggga
     6661 tttactttgg agatcatatt cttgaagagc gcaatgcgcg tgatatcaac gactatagct
     6721 atgaggaagt ggagcggatt attcgcaaag cctttgaaat tgcaagaaat cgcagaaaaa
     6781 tccttactag tatcgataag caaaatgttc tagcgacctc aaaactctgg cggaaagtag
     6841 ctgaggaagt cgcacaggat ttctcagatg taaccttgga acaccagctg gtagactcag
     6901 ctgctatgct tatgattacc aatcctgcta agtttgatgt tattgtaacg gagaatcttt
     6961 ttggagatat tttatctgat gaatcaagcg tcttatctgg tacacttggg gttatgccat
     7021 cagccagtca ttctgaaaat ggaccaagtc tctatgaacc tattcacggt tcagtacctg
     7081 atattgcagg tcaaggaatt gccaatccta tttccatgat tttatcagtt tccatgatgt
     7141 taagagatag tttcggaggt tatgaggatg cagagcgtat caaacgtgct gttgagacaa
     7201 gtttggcggc aggaatttta acgagagata taggaggtca ggcttcaaca aaggaaatga
     7261 cggaagctat tattgcaagg ttatgaagtt agacgaaaaa attactctag tccttttgat
     7321 ttggaatgtc atcattttct tgatttatgg cattgacaaa ttcaaggcaa gaagaagagc
     7381 ttggcgcatc ccagagaaaa tcttactcat tttagccctt acttgtggtg gttttggggc
     7441 ctggttagct ggaatcactt ttcaccacaa gactcgaaaa tggtacttta aaacagtttg
     7501 gtttcttggg atggtgacca cactagtagc cttatatttt atttggaggt aatggatggc
     7561 agggtcttcg agggaacacg ctgcttgggc tctagcggac tatggtttta aggtcgtgat
     7621 tgcaggatct ttcggtgaca ttcattacaa taatgaactc aataatggca tgttgccaat
     7681 cgttcagcct agagaggtta gagagaaact agcccagcta aaaccaaccg accaggtaac
     7741 tgtggacttg gaacaacaaa aaatcatctc accagttgaa gaattcacct tcgagataga
     7801 tagcgagtgg aaacataaac tcctaaatag tttggatgat atcggtatta ccttgcagta
     7861 tgaagagttg attgctgctt atgaaaaaca acgaccagcc tactggcagg attagaaaaa
     7921 atagaaaagg aaatatagta aactgaaata agatgtaaac aaatgaattg gagcttaaca
     7981 tccatttcca gcaatttttt agaaactaca gtggactatt ctggattcaa cacattataa
     8041 aattatgaca aaacacattc acaagaaggc tacgacattt taaaaggtga gggcggatgt
     8101 atcgtttgcc ctactaaagt tggttacatt atcatgacca gcgacaaggc aggacttgag
     8161 cgtaagtttg cagccaaaga aggtaagcgt aacaaaccag gtgttgttct ctgcggtagc
     8221 atggatgaac tttgcgcttt agcgcaactc aacccaaaaa ttgaagcatt ctactaaaaa
     8281 cattgggatg aagatattct tcttggttgt atccttcctt ggaaaccaga agcctttgaa
     8341 aaactcaaag catacgggga tggccgtgaa gaacttatga ctgatgtacg tggtactagc
     8401 tgttttgtta tcaagtttgg aaaagcaggt gaacaattgg ctgccaagct ttgggaagaa
     8461 ggtaaaatgg tctacgcctc atatgcttca atgacaaaac gattgaaact cgctatgaga
     8521 gcaaggtgta atggtgtcta tggtcgataa ggacggcaaa ctcatcccag aacaaggagg
     8581 agcacgttca acctcaccag caccagttgt gatccgtaaa gggcttgaca ttgataaaat
     8641 catgatgcac ctgtcagaca cctttaactt atgggactac cgtcaggttg agtattatta
     8701 ggatagagaa gaagtctagt gttaaggaca ttacagctca taatactagg cttttttata
     8761 gaaattcttt tctt
//
LOCUS       DAVLYR010000078         8704 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLYR010000078 DAVLYR010000000
VERSION     DAVLYR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.078"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            83..154
                     /locus_tag="VYO63_001328"
     tRNA            83..154
                     /locus_tag="VYO63_001328"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:116..118,aa:Glu,seq:ttc)
     gene            160..233
                     /locus_tag="VYO63_001329"
     tRNA            160..233
                     /locus_tag="VYO63_001329"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:194..196,aa:Asn,seq:gtt)
     gene            complement(274..816)
                     /locus_tag="VYO63_001330"
     CDS             complement(274..816)
                     /locus_tag="VYO63_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1672242.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(1076..2329)
                     /locus_tag="VYO63_001331"
     CDS             complement(1076..2329)
                     /locus_tag="VYO63_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1672243.1"
                     /translation="MKCFVGLDISSTKLDVCIMLSDTSTPVTASLSNDLSGATEIKNH
                     ILDLNHTYHFERIVIGMEATSLYSFHPAMFFHEDRQLKELHVEIMVEQPNKIKKYRDA
                     FEENKNDTIDAFYIADYFRIERFSPSFLKEEKYMALQHLTRTRLQIIEQLTRTKQHFI
                     ENIYYKCNTLAYQFKKEELSTNLWSSTMMTLMTDEMTLDELANMPLQELDDWLQKLGK
                     GHFKDSEKLAQTIQAAIRGSYRLSKLQQDSVNVILGLLAREIRNLEKMIKEIDKAIED
                     MVETIPEYQCLTSIPGVGKVYAAGIIAEIGQIERFKDHPQVAKFAGLNWREKQSGNSN
                     SQHTSLVNRGNRYLRYYLVEAANSVRRYDDEYKDFYKKKYHEVPKHQHKRAILLTARK
                     FVRLVDVLLRNHQLYTPPRRIMEDN"
     gene            2685..4443
                     /locus_tag="VYO63_001332"
                     /pseudo
     CDS             2685..4443
                     /locus_tag="VYO63_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(4473..7016)
                     /locus_tag="VYO63_001333"
     CDS             complement(4473..7016)
                     /locus_tag="VYO63_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1672244.1"
                     /translation="MKSFLKTYRTYFISLIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLSPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYTLMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVLFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSTYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVDFDY
                     FSPIPYEKIENTNNLISVTSSSNEDAAIQYQIEVPENSQIYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7078..8700)
                     /locus_tag="VYO63_001334"
     CDS             complement(7078..8700)
                     /locus_tag="VYO63_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1672245.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         2796 a         1484 c         1667 g         2757 t
ORIGIN      
        1 acatatttta gagaaaaaat ctcaaaagtg attgacaatt agcaagaaat tgatataatg
       61 gttatatctg ctacagatag aaggtccgtt ggtcaagggg ttaagacacc gccttttcac
      121 ggcggtaaca cgggttcgaa tcccgtacgg actattttat ccgccatagc tcagttggta
      181 gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactggcg gagtatagat
      241 aaaagggaac acagctgtgt tcctcttttt gtatcaattt gtatcaccaa gcattttcat
      301 aaggaagtct gttatttctt gagaactttc tttttttcca tgtgcaatcc aagtttggca
      361 gacaccaaaa agtgcatgag ttagatagat gctactatat tctaattcag tggtatttag
      421 attcagttgc ataaatcgct tttgtaaatc tgtactaagc atgatatgaa gtttatttcg
      481 taagaaattt tggatttctt tagtcccatt ttcagaaaga agggcagcca gaagtggttc
      541 tgactctaga tattcaaaaa cttctaaaat agcgtctctt ttgtgatgag catgtttttg
      601 aaaaatatat tcaaatgtat ggaatagctt gctttgatag tgctcaatca tatcatactt
      661 atccttatag tgagtataga agctggaacg actaattccg gctttttcta ctaatttgac
      721 agtagaaatt ttatcaaatg gctgttccat cagtaattgt accatagcgt tttcaatagt
      781 tcgctttgtt tttaagcgtt tgttactttc ttgcatattt cctccttgta aacaaattag
      841 actatatgtc taaaaataga ttttttatct tgtaatttag attttttaat gtataatcta
      901 ttatatcaaa attttagaca atatgtttaa aaaagtctag tggtaaaatg tcaagcgacc
      961 ccccgctcaa agtttgaaaa ctatttcaaa aaaagggtgc actaaagaag ccctactgaa
     1021 aacgatgttt tcacactgtt tttagtagat tttcaaggag aggtgcagat ataacttagt
     1081 tatcttccat aatcctcctt ggtggcgtat agagttgatg gttgcgtagt agcacatcca
     1141 ccagacgcac aaattttcta gcggttagta agatggctcg tttgtgttgg tgtttaggta
     1201 cttcatggta tttcttctta tagaaatcct tgtattcgtc atcataacgc cttaccgagt
     1261 tggcggcttc aaccaggtag tagcgcagat aacgattgcc cctattcaca agcgaagtat
     1321 gttgtgagtt tgagttacca gattgctttt ctctccaatt cagaccagca aattttgcga
     1381 cctggggatg gtctttaaag cgttcgattt gtccaatttc ggcaatgata ccagcagcgt
     1441 agaccttccc gaccccaggt atggaggtca ggcattgata ttcaggaatg gtttccacca
     1501 tatcttcaat ggccttatca atttccttga tcattttttc caggtttcga atttcccgag
     1561 ccagaagtcc gagaatgaca ttgactgaat cttgttggag tttagagagg cgataagaac
     1621 ctctgatagc ggcttgaatg gtttgagcaa gcttctcaga gtccttaaaa tgccctttac
     1681 ccagtttttg gagccaatca tctaattctt gaagaggcat gttagcaagt tcgtctaagg
     1741 tcatctcatc tgtcatcaag gtcatcatcg ttgaactcca taggttagta gagagttctt
     1801 ctttcttgaa ttgataagcc aaggtattgc acttgtagta gatattttca atgaagtgct
     1861 gtttggttct ggttaattgc tcgatgattt ggagtctggt tcttgtgaga tgttggagag
     1921 ccatgtattt ctcttctttt aaaaaagaag gtgaaaatcg ctcaatacgg aaataatccg
     1981 caatgtagaa agcatcaatg gtatcgtttt tgttttcttc gaaagcatcc cgatacttct
     2041 tgattttatt aggttgttcg accataatct caacatgtag ttctttcaac tgcctatctt
     2101 catggaagaa catagcaggg tggaaactgt agagactggt tgcttccatg ccaatgacaa
     2161 tcctttcgaa gtgataggta tgattcaaat caaggatatg attcttaatc tctgttgccc
     2221 cagaaaggtc attagagagg gaagcagtga cgggggtgct agtatcactc aacatgatac
     2281 acacatctaa ttttgtagag ctgatgtcta aaccgacaaa acatttcatt aaggaaggtc
     2341 tcctttccat tagatttgga agtttttttg actactgtaa ggtttcccag aaacaccttg
     2401 ttccaacatc ctcgcataag agaatcctta tcacagcctg acagagccgc ctgcatccta
     2461 ctagatacag aagtcaagct gtggaaacag ctagtgtgtt ggaagtttga acaaggcttg
     2521 ggtaacagac tttttaatga agtccaagca acaaggagaa aaagaaacaa ccatgagtcc
     2581 attccatgac tctattgttc tagaaaacct tgcaatagtc aagtgataac gaataaaact
     2641 tgggattgaa atatccccat gattatatat tacgaggaga aactatgttt aaagaatgga
     2701 aagcaattta aaaaaaacaa cctttattat tgtcatgatc gggatttctc ttattccagc
     2761 tctgtacaat atcatatttt tgtcatcaat gtgggatcca tatgggcaat tgtctgactt
     2821 acctgtggca gttgtaaata atgataaaga ggcttcctat aatggtaata ctatggcaat
     2881 aggaaaagac atggtgtcca atttaaaaga aaataaaacc ttggattttc attttgtaga
     2941 tgaagaggaa ggaaagaagg gattggaaga tggcgattac tatatggtag tgactttacc
     3001 aagtgattta tctgaaaaag caactacatt atccaatatt caatcgacag cagcttatca
     3061 atcattgaca agtgagcaac aaactgagat aagtgattct gtatctcaaa attcaactga
     3121 tagtattcaa tcggctcagt caattgtagc tttagtacaa gatttacagg gaagtttaga
     3181 aaacttacaa aatcaatctt ctaatctttc gactttaaaa aatcaagcta atcaagtatt
     3241 acctattact tctacttctt tgataggatt gtcaagtgga ttaacagaga tacaaggagc
     3301 tgttactagc aaattagttc ctgccagtca gtcgattgca tcaggtgtaa acgcatatac
     3361 tacaggtgtt gataaagttt ctcagggcgc aagtcaacta agtgaaaaaa atgctacctt
     3421 gacaggtagt ttggatcaat tagtttcagg ctcaaacacc ttgacacaaa aatcttctag
     3481 cttgacagca ggagttggtc aattagttga aaaaactcca gaattagtat ctggtattga
     3541 aaaattatca actggctcca attaattgaa tcaaaagagt caagaattga tagcaggagt
     3601 tgataaattg cagtcaggct ctagccaact agctgacaaa tccagtcagt tactttcagg
     3661 tgtttctcaa ttagaaagtg gagctaataa attggcagat ggagctggga aactagcaga
     3721 aggtggaaca aagttaactt ctggattgga aggtttacag acaggacttg cttctttagg
     3781 acaaggacta ggtaatgcta gtgatcaact caaatcagca tcaacagaat ctaaaaatgc
     3841 agagattttg tcaaatccac tcaatctttc caaaacagac aatgaccaag ttcctgtaaa
     3901 tggaattgca atggctcctt atatgatatc agttgctctt tttgttgcag caatatcaac
     3961 gaatatgatc tttgcgaagt taccttcagg acgtcatcca gagagccgtt gggcttggtt
     4021 gaaatctcga gctgaaataa atggtattat agctgttttg gcagggattt tggtatatgg
     4081 aggagttcat cttattggtc taactgctaa tcatgaaatg agaacattta ttctcatcat
     4141 cttaacaagt ttagtattca tgtctatggt gaccgcttta acaacatgga atagccgtat
     4201 aggagctttc ttctcgctta tttttctttt attacagtta gcatcaagtg caggtaccta
     4261 tccgcttgct ttgacaaatg atttctttag agctattaat ccttggttgc caatgagtta
     4321 ttcagtttcg ggattacgac aaacaatctc catcaacaag tcattttcct agctgtcata
     4381 ctagctctat ttactggttt aggtatgcta gcctatcaac ctaagaaaat ggaagaagat
     4441 taaaaaaatc gaccgattaa ctggtcgatt ttttatgcct tagatgactt tcgtctgtga
     4501 ttatagattc caaatagtaa gagagaagta aaggaacaga ttgctccagt aataaaacca
     4561 ttgggaatga aggaaagtgt aatagttcct ttccccttgg gaatgtcaac tttcataaat
     4621 ccagtttgag cttgtttaat ttctattttc ttaccatctt ggtaggcaga ccaacctttg
     4681 tcataaggaa tggtgaagaa aatagatgta tcttgttgga catcatatgt agcaaaaacc
     4741 ttgtttttag aagttgatac tgtgacaggt tgttctttaa ttttttgaat tgcctcggtg
     4801 aaagttttgg tatctaaacg atagaaggta ggagattcaa atgatacttg tgaatttcca
     4861 gggaaactaa cattgatatt gaaagttttt ttctctttag tatatcctag attaaagaag
     4921 gagaagacat tatcagttgt aaaagtcttt ttttcaccat ttacaaggat gtcaaccttc
     4981 ttttgtttat cgttagaaaa gtgaaggttt atgaaagaga gataaatttg gctgttttct
     5041 ggaacttcaa tttgatactg gattgctgca tcctcatttg aagaacttgt gacactaatc
     5101 aaattattag tattttctat tttttcataa gggattggag aaaaataatc aaaatcaacg
     5161 ttagcaagtt gatttaaaaa cgaggcctga ttatccaagg tatgttcatt gaacttgaca
     5221 tcattgtaaa cagattgact agcaactgca atcggaagag agtattgatt ttcatatagg
     5281 gtaagattat ctttttgata gatatcttta aaaccatact tatcaatagg actgtctgag
     5341 atattgtact ggataccaaa taaactatca gccaaaatac tattatttgc atatcggaga
     5401 ttgagattag tcccagagga tttaaaccca agtttatcta aagtagagct tgatgaacga
     5461 tttcgaacag atgaaaattg agagattcca ttgtagttga atttcatact gtcatttcct
     5521 gtctgagttt gtagtttttc agtacgagta aattgatttc caatatatgt tgagaaagat
     5581 tccatagctg ggatatctcg actataagta cttcgagaag caaatcccca ttccttagca
     5641 attccgtcca tttgagatga agcatttaaa ctcatttcaa ccagtataaa taaagagatt
     5701 agaatggcaa atagattcac agatataaac tttttgataa ctgcaaggag taaaagagaa
     5761 tagacaacca aaaattcaag agtaagcaga atattcaaat ctgttaaaaa agaataatgc
     5821 gattttagat agatggtagc taaaaatcct gttactacaa gaaaaagcga aactaaaaaa
     5881 ttccagactt taagttcttt cagacgcttt aagacttctg ctgctgtgta aattaacaag
     5941 gtagagaaaa tccaagcata gcgatgtaaa aacatgtttg gagtatgcat gccttgccaa
     6001 aataagtcaa gagcttctat gtaaaagctt gcaattagaa atgcaaagaa taatacatat
     6061 atgagtttca cgtgaaactt aatagatttc agcgtaaaaa ataaaatggt caaaataaaa
     6121 ggaagtagtc caacaaaaat cattgggatg gccccatact ttgttgtgtc aaaggaacca
     6181 atgaattgct tagcaaagag atcaagatac cagctacttt cagtttgaaa ctttgtaact
     6241 tcagtcaatt tttccccatg tgtctgtaaa tcaaacagag tgggaagagt cataatcaaa
     6301 ctagccatac cagctaaaaa ggagataact atgaaatcaa gaacagatga ttttcgagtc
     6361 ttaaagtccc acgaaatttg acagagatac cagaaaataa gaaacaatac tgtcatatat
     6421 ccaaaataat aattttgaat aaataagatt gacagacttg taaagtacaa taggagtttc
     6481 ttttcagtta tcagtagatg taaaccagtt ataattaaag gaatcaagat aaaaacatct
     6541 agccaggttt ttatctctaa ttgactgaca gtgaaactca tcagagtata ggaagtagat
     6601 aaggctagtt ttaaagtctg agggatagat tgaaacaatt tattcaaact aaaaaaggtt
     6661 gacagaccaa tcaatccaaa ttttaagaga gtggtcagat aaacagcatc tggcatattc
     6721 gttagatcaa aaaagtaaac cagaggtgag agaaaactac ccaagtaata actagacaga
     6781 gcatagaagt ttagccctag accacttgta aaggtgtaaa acagactact atttccatgt
     6841 aggatatttc gtaaggctac atcaaaaata acgtattgat gaaagccatc tcctaataga
     6901 ggagagttgt cgctattcca gtagatacct tgagatagat atactccaga cataatcact
     6961 acaggaatga tgagagaaat aaaataggtt cgatatgttt ttaaaaatga tttcatgtta
     7021 cctcgtagaa tgatagaaaa ctcagttggt taacccaact gagttttgaa gttttattta
     7081 gtctttccaa agttctttaa cttttgcttg tacttctgca ttttctagga attcatcgta
     7141 ggtttcatcg atacggtcaa tgacgccatt tttagataag acaatgatat ggttagccaa
     7201 agtttgaata aattcgtggt catggctagc aaagatgatt gattctttaa agtttttcaa
     7261 tccatcattc aagcttgaga tagattccaa gtccaagtga tttgttggat catcaagtac
     7321 aaggacattt gattttaaga gcatgagttt tgaaagcatg acacgaactt tttctccccc
     7381 tgacaagaca tttacaggtt tgttaacttc atctccagag aagagcatac ggccgaggaa
     7441 gccacgtagg aaagtattgt catcttcttc tttacttgcg aattgacgca accagtcaag
     7501 aattgattct cctcctgcaa aatcagctga gttatctttt ggtaggtaag attgactagt
     7561 tgtaactccc cacttgacag ttccttcata gtcaatatct cccatgattg cacgaattaa
     7621 tgcagtcgtt tgaatatcat tttgtccaat aagtgctgtc ttatcatctg gacgcaagat
     7681 gaaactaata ttatccaaga tagtttcacc atcaatcttt acagttagat tttctactgt
     7741 caagagatca ttaccaatct cacgttccgc tttaaagttg ataaatggat atttacgact
     7801 agatggtaca atctcttcta gctcaatctt atcaagcatt ttcttacgtg atgttgcctg
     7861 ccttgactta gaagcattgg cagagaaacg agcaacgaat tcttgcaatt gtttaatttt
     7921 ttcttctgct ttagcattac ggtctgctag caatttagca gcaagctcag aagattcctt
     7981 ccagaagtcg tagtttccga catagagttt gatttttcca aagtcaaggt cggccatgtg
     8041 agtacaaact ttgtttaaga agtgacggtc gtgggatact acgataactg tgttatcaaa
     8101 gtcaatcaag aagtcttcta accaagtaat cgattggata tccaaaccgt tagtaggctc
     8161 gtccaagaga agaacatctg gtttaccaaa aagtgctttg gcgaggagaa cctttacttt
     8221 ttcaccgttg gccaattcgc tcatgttttg gtagtgtaat tcttctggaa tgtttaggtt
     8281 ttgaagtagt tgagaggctt cactctctgc ttcccaacct ccaagttcgg caaactctcc
     8341 ttcgagttcg gcagcacgaa ccccgtcctc gtctgagaaa tcttccttca tgtagatagc
     8401 atctttctct ttcatgatgc tataaagttt ttcatttccc atgataacga catcaatggc
     8461 acgttcatct tcgtagtcaa agtgattttg acgaagaaca gagagacgtt catctggacc
     8521 aagagagatg tgaccagtag taggttcgat atctccagct aaaattttta aaaaggttga
     8581 ttttccggca ccattagcac cgattaatcc gtaagtattt ccttctgtaa atttgatatt
     8641 gacatcatca aaaagtttgc gatcactaaa acgtagtgaa acatcagata ctgtaagcaa
     8701 tatt
//
LOCUS       DAVLYR010000079         8600 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLYR010000079 DAVLYR010000000
VERSION     DAVLYR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.079"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            89..541
                     /locus_tag="VYO63_001335"
     CDS             89..541
                     /locus_tag="VYO63_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1672246.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(576..1964)
                     /locus_tag="VYO63_001336"
     CDS             complement(576..1964)
                     /locus_tag="VYO63_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1672247.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2030..2992
                     /locus_tag="VYO63_001337"
     CDS             2030..2992
                     /locus_tag="VYO63_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1672248.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKNYMEG"
     gene            complement(3021..3179)
                     /locus_tag="VYO63_001338"
     CDS             complement(3021..3179)
                     /locus_tag="VYO63_001338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672249.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHVFEVVDETDEVS
                     NHTYGKAT"
     gene            complement(3243..4253)
                     /gene="ccpA"
                     /locus_tag="VYO63_001339"
     CDS             complement(3243..4253)
                     /gene="ccpA"
                     /locus_tag="VYO63_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1672250.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4678..5277)
                     /locus_tag="VYO63_001340"
     CDS             complement(4678..5277)
                     /locus_tag="VYO63_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672251.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5279..6376)
                     /locus_tag="VYO63_001341"
     CDS             complement(5279..6376)
                     /locus_tag="VYO63_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1672252.1"
                     /translation="MLERLKSIHYMFWVSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VEQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     EAMTSMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(6376..7113)
                     /locus_tag="VYO63_001342"
     CDS             complement(6376..7113)
                     /locus_tag="VYO63_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672253.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(7115..7999)
                     /locus_tag="VYO63_001343"
     CDS             complement(7115..7999)
                     /locus_tag="VYO63_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672254.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(7986..8180)
                     /locus_tag="VYO63_001344"
     CDS             complement(7986..8180)
                     /locus_tag="VYO63_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672255.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIHGALGFGFM
                     IFVVFPMGMEWLKGESHDGG"
     gene            complement(8177..8383)
                     /locus_tag="VYO63_001345"
     CDS             complement(8177..8383)
                     /locus_tag="VYO63_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672256.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         2600 a         1985 c         1539 g         2476 t
ORIGIN      
        1 aatattcttt tgaaaaatac tttttttgct ttacttctat agtgaaaagg tttacaatta
       61 tagtaagaaa atttcaggag gtttcattat ggcacaacgt taccaaaata tcatggtcgc
      121 aatcgatggt tctaaggaag cggacttggc ttttgtcaag ggagttcatt ctgctctacg
      181 aaacgacgct aaactcacca tcgcacatgt cattgacaca cgcgctctcc aaagcgtatc
      241 cacctttgat gctgaagttt acgaagaact ccaagtcgac gctgaaagtc tgatgaaaga
      301 gtacgaaaaa cgtgctaaag atgctggggt agcagatgtt catatcgtca ttgaaatggg
      361 aaatccaaag accctgctag cacgtactat tccagatgcc gaggaagtgg acctcatcct
      421 cgttggcgca actggtctca acgcctttga acgcctcttg gtcggctctt catctgaata
      481 catactccgc catgctaagg tcgatttgct ggttgtgaga gaacaagaaa aaaccttata
      541 atcacaaaga aaaggagccc ctagctcctt tttgtttacg atttatttct ctctttatgg
      601 cgttcgtaag ccttgagctg gcgctgcagt tcctttttaa tagcaggttc tggagcatat
      661 ttttcttccc aattatctgg ttttaagatt ttatgggtca ctggatcaaa atgagccttg
      721 ccatctggaa aaattttccc catattggcc tgatggacaa tatcaaaaat acgttctggg
      781 tccaccccca tcaagacaaa actgccgtag gtgaagtaaa gcgtgtcaat caaggcatcc
      841 acttgcccta tcaaatcttg ctgagcaggt gtcttcttgg ctactttacc tgctgcctta
      901 tcaagggcct gatgaagttg cgatacagct tgaccaaaat cttcttcaga aggactggct
      961 gatcgaacaa actccaccaa ttcttctatt ttaaaaccag ccctatgggt tgcaccctct
     1021 aaatcccaag ctcgaggttc ttcttgggtt cgttcatcca tcatgtggtg gaaggtcttg
     1081 accttattga aatgatagtc acggctgaca aaaacttttt ctgaagacaa aacaccaaaa
     1141 tgttccaagg ccttgtggat cccatcttgt tgattgctag tggtaatgtg cttggcagct
     1201 tccttgacac tgctactacc atttcccata gcgaccgaca taccgacgcc agccagcatt
     1261 tcaaggtcat tatctgagtc accaaaagcc atgacttggt tgaggtcaaa gccatattct
     1321 ttcccaactc ggcgaatgcc ttctaattta gaatttccct gattgatgac atccgatgca
     1381 aaaggattgc tacgtgtcaa tttcaagtct tcaaaatcag ctgccgcctt ctcagattct
     1441 tctggtgtca tcagcatcaa aacttggtaa ataggttgat tcatcaggtg aagcaggtcc
     1501 tcttcctttt ggggaacaac cttgctgacc atgcgattaa aagactgact caccgtccga
     1561 gttaaaacag agggaacgaa gcgactaatt cgttgggaaa aagaacccag accaaaggac
     1621 atgattttag aacccaacat ggcatccttg gtccctaagg caatctccgt gccctctttt
     1681 ttagcatagc taattagatg gcgcaaatgt aacttggaaa tagggctcgt gaacaagact
     1741 ctgtctttac taaagatata ctggccatta taggttaccg caaaatccag atccaaatcg
     1801 tccatcaatt ccttaacaaa aaaaggtcct cgccctgtcg ctacgccaac tagtacccct
     1861 tgttctttga caatcttaat cgcgtcctta gtggatttca aaacactctt gcgattgttg
     1921 accaaggttc catcgatatc aaaaaaaaca gctttgactt ccatcctatc ccaatctccc
     1981 cttttgtgat acaatgatta taccacattt cagaaagagt gagtaaatca tgcctaagaa
     2041 aatccttgtt ttacatacgg gtggaactat ttccatgcag gccgatgctt ctggcgctgt
     2101 tgtgacgagt tcagataatc ccatgaacca tgtgtccaac ccacttgaag gaatccaagt
     2161 ccacgccttg gactttttta accttccaag tccccatatc aaacccaaac atatgctggt
     2221 cctctaccag aaaattaaag aggaagcaga taactacgat ggagtggtga tcacacacgg
     2281 aaccgatact ttagaggaaa cagcctattt ccttgatacc atggaagttc cccatatgcc
     2341 tatcgttcta acaggagcca tgcgtagctc caatgagctc ggtagtgatg gtgtttataa
     2401 ttacctaagt gctttacgag tggccagcga tgacagggct gctgacaaag gagttttggt
     2461 cgttatgaac gatgaaatcc acgctgccaa gtatgtcacc aaaacacata cgactaatgt
     2521 cagcaccttc cagactccaa cacatggccc cctcggtctc atcatgaaac aggaaatcct
     2581 ctacttcaaa acagctgaac ctcgtgttcg ctttgacctt gatcacatac aaggtttagt
     2641 ccctatcatc tcggcttatg ctggtatgac agatgagctg attgatatgc tggatttaga
     2701 acacttggac ggtttgatta tccaagcctt cggagctggt aatattccca aagaaacggc
     2761 tcaaaaatta gaaagccttc tgcaaaaagg aattccagtc gctctggtat cacgatgctt
     2821 taacggtatt gccgagcctg tttatgccta ccagggtggg ggcgtacagt tgcaaaaagc
     2881 aggcgttttc tttgttaaag aactcaacgc ccaaaaagct cgcttgaaac tcctcatcgc
     2941 cctcaatgcc ggactaacag gacaggcttt gaaaaactat atggaaggct aatactcttc
     3001 gaaaatctct gcaaaccacg tcacgtcgcc ttaccgtatg tatggttact gacttcgtca
     3061 gtttcatcta caacctcaaa aacatgtttt gagctgactt cgtcagttct atctacaacc
     3121 tcaaaacagt gtgttgcgca gcctttaacc agtcgcctag tccgctctat ggtattcatt
     3181 aagtcaacat ctcttgttta agagcacaaa atcaggaaat cttctcgatt ccctgatttt
     3241 ttctatttac gttttcgtgt tgagctacgt tctgtcaaac catgaggtaa gagaacttca
     3301 cgttcttcca actcttcctt atgcataatc ttggtcaaca tacgcatact aatggcacca
     3361 agatcataaa gaggttgggc aatcgttgtc aagtttggac gggtaaagcg tgagatttgt
     3421 gaatcatcac tagtaataat ttcaaaatct tctggcacag aaacaccctt atcagccaaa
     3481 ccgttcaaga ctcctgctgc caactcatca cctgtcacaa ctgctgcagt tgcatttgat
     3541 gaaatcaaac gctctgctaa ggcgtaacca tcatcatagc tatatttaga ttcaaatacc
     3601 aaaccctcac tataagtgat tcctgctttt ttcaaggttt ccttgtagcc aactaaacga
     3661 accttaccat tgatgtcatc cactagcgga ccgctaacga aagcaatacg ctcattttct
     3721 ttagcaaggt aactcactgc atcaattgtt gcttgcttat agtcaatatt gacacttggc
     3781 aactggtgct caacatcgac agttcctgcg agaacaatcg gagtacgtga acgcgaaaat
     3841 tctgagcgaa ttttatctgt caagtgataa cccatataga taatgccatc tacctgcttt
     3901 gaaaagaggg tattgacaac agaaacttct ttctcgttat cttcatcgct attagctagg
     3961 acaatattgt acttgtacat ttctgcaata tcatcaatcc ccttagccaa actcgaaaaa
     4021 taaccattgg taatatttgg aatcacgaca ccgacagtgg ttgtcttttt acttgcaaga
     4081 ccacgcgcaa ctgcatttgg acgataatcc aaacgatcaa ttacctctag cactttttta
     4141 cgggtattct cttttacatt tttattgcca ttgaccacac ggctgaccgt cgccatggaa
     4201 acacctgctt cacgagcgac atcataaatg gttactgtat catctgcatt cattcctttt
     4261 cctgtccttt ctatctcaca cattctttta caagtagagg tactgattga agctctatat
     4321 ctacttacaa aagtgaagat gtgaaaattt cgttttcata tttctactta ttccattcta
     4381 tcactaattg taaacacttt caagtgtttt ttgaagattg attgaaaaaa tttcatagaa
     4441 aacctaggtt tagctccttg ctaccacctt agactaaaca caaaggagga aactaagccc
     4501 tcctaaagtt atagtaaaat gaaataagaa caggataaat cgatcaggac agtcaaatcg
     4561 atttctaaca atgttttaga agtagaggtg tactattcta gtttcaatct actataggta
     4621 ttgttccatt cactaccgtc aattttagca catagccttc ataaaaatat tatatcatca
     4681 taaccaacca gattcttttg cgatattagc tgcctctgtt cgattaccag catccagttt
     4741 tgaaagaata ttggtgacat agtttcgaat agttccgttt gagaggtaaa gctgatctgc
     4801 gatttcttgg ttggataagc cccgggcgat tccctttaag acagcaatct cttgttctgt
     4861 caacggattg ggacgggtca tcaccacttc catcaattca ggcgaatact ccttgcgtcc
     4921 ttcgaggacg gtgtgcaagg tttgcatgag gtctgcaatg cttcgttcct ttaatacata
     4981 agcatccact ccagccttga ccgcacgttc aaaataccca gcacgcttga aggtcgtcac
     5041 cacaaccacc tttgtttcaa gcctttctga tcgtatccac tccaagactt caagacctgt
     5101 cttaacaggc atttctacgt caaggatggc gatatctaca gactcctttt ctaatagttg
     5161 gattgcttct tgcccattct tggcttgaag gacagactcc acatctggtt gaagcgtgag
     5221 caattgacac atggcatctc gcaacatact ttgatcttct gcgactaata ctttcatctt
     5281 agtttctctc cttataaggt agtcgaactt gcacttccgt tggatgtttc tgactgatta
     5341 cacttacttc tcctgaaaat ggaaaaacac gatctcggac tgtatggagc tcatctcctt
     5401 ttagaaaagc aaagccgcag ccatcatcac ttactgttaa aatgagttcc ttctctgtcc
     5461 gttctaattt taagtaagct ttagacgctt tggcatgttt gatgatattg gtcaccaact
     5521 caagcaaaat catagaagtc attgcctcca attcttgagt aaggctagca gtatctagtt
     5581 ggttatccgt ttccacctca attccagcaa tttctaacat ctttttcaca gtctctagtt
     5641 cggatgtcaa agttctagac ttaagatttt ccacaatggt tcgaacttca cgcatcgatt
     5701 ctttgctaat ttgctgtatt tctcttaatt ccttttccac ttgtggataa gcctgcattt
     5761 gaaatagctg caaggctaga tctgtcttga cactaagcat agcaaaggta tgtcccagac
     5821 tatcatgcaa atcctgaccg atacgattgc gctcattttc tgctagcaat agatttatct
     5881 gggtattttg ctcgacctga gcttctttca aatcctcgac aaggcgaatt cgaaccgagc
     5941 ccaaagtcat caaatcgaca aaagtaagaa ttacaagcat atagactaga aactcaactt
     6001 cgattctctg aaaaatcaaa agtcgcccca caacaaggac ttgagcaagg agaaatgtcc
     6061 atacatgtaa agactttaaa ctacgtacgc caaaatgata aattaagaga ttggaaagga
     6121 aaaagaaaaa ccagatataa ttcccagcaa caaaggcggt attcccagct acatagacca
     6181 gcatgatccc ccagaaaagc caagataggc gctggttctt agttattaaa acacccaaat
     6241 acgtcactac aaatagaata tcaaccaata aatgccaagc aggaagctcc ccaatcacta
     6301 caggtaggat gggaaaaagc ataaaaatca aactgaccca aaacatatag tgtatgcttt
     6361 tcagtctttc aagcattaag cattctcctt atgaccttga aggtaaatag tcaaaccaaa
     6421 taaaactgct gaaaaaacaa gtaaataaac tgtggctgat agattgatgc caccctcgtt
     6481 taagaaggtc ttgagcaact ccatcaattg ataggtcggg agacacttac caactgcttg
     6541 catccagtct ggaaataaag aaatgggcat ccagagtccg cctaaaacag ccaatcctag
     6601 ataaagaaga ttgcccacaa cagacatcag ctgactagtt ggtaagagag tcaaggtcaa
     6661 gccaagtgct acaaaagcaa tactccccac aatcagcaaa atcgcagctc caacccattt
     6721 tactagaggc atgtctacac ctcttacaaa atgtccaact gtaaaaacaa ccagaattga
     6781 gaccaaataa tcaaccagca tacttgttat ctttgataga taatattcta ccatatttac
     6841 agggctatga cgcaatgttt tctgccagtt gttcatctta tcggtatgta aaacagctgg
     6901 aaatgagagg atagttgtcg acatcatgga aaaggcagtc atggagatga ggtaatcacg
     6961 cataaaattc gcgggttcac ctggtgtgtc ctgatagata ccagaaaaaa ataaatagaa
     7021 agccgtcggc atccctaccg acaatagata atagattaat tgtcgtttgg tcaataaaaa
     7081 ttctatctta ctaagtgcta tccatcgttt catcttagtt atctcccttc tgcgtctctt
     7141 caaagattgt attcaacaaa ctacgattat taacttcgat ttcttgcatc ctacatcctg
     7201 cttgggctaa tagttcccag aaagcatctg cttctcgagt gactacttgt aaagaatctt
     7261 gctttagggt ccagttttca accaaatttg actgctctac gacttccttg taagctatag
     7321 gaaggataaa gtgcttttct atttcctcgc tacgcatagc tagaggcgtc gtatctcgaa
     7381 tcaactctcc cttatttaag agcaaaatgc ggtctgcagt gtgttccact tcctcaatat
     7441 aatgggacga atagagaatg gtgactcctt gcgcttttaa ctcctggaca atttcccaaa
     7501 aacgttgacg agttgaggta tccatggacg cagtaggctc atctaaaaaa acaatctttg
     7561 gtcgcccaat taaggtcaag atgaaagaaa agagacgctt ttgaccgcct gataattttt
     7621 ctgccaactg ttctttttgt tgcttgacaa actgcaatag ttgatcaatt tcctgattgc
     7681 tcaaaggatt tggatagata cgttgaaaga aagcaatcaa ctctttaact tttaatttct
     7741 gaacgattac attttcttga gggaggtaag ccctagtata gtctaactta gaactcgtca
     7801 ctggtaaacc ttgaatagat acttgaccgc ttgtgaccag tttatctcca agcaaacaat
     7861 ctaagagtgt tgtctttccg gcaccatttg gaccaattaa ggcaacacat tcaccatcgt
     7921 tgatttcaaa ggagatgttc cgcaaaattt ccttgtcttt tattttctta ctcaatttct
     7981 caactttaac caccgtcatg agactctcct ttcaaccact ccattcccat agggaaaacg
     8041 acgaaaatca taaatccaaa ccccaaagca ccatgaatga attggcgaag caaggcttga
     8101 tcaaaccaat cagtaaacat ttccactaac cataccaaga gtgacaggcc gataaagaaa
     8161 tagatgatcc ctctcttcat tcctcaaact cctttttcac atctctgact aatttcaaac
     8221 cttctctaac aagccaagac atgattccaa agccagcaaa gaccttccaa ggaaaatggt
     8281 agaaatcctc atccaatccc gaaaacatga gataggtcat aactcctgct actactaaac
     8341 tcattgcaat aatcatttta tttctcatct cttcttcccc catttcatac tacaattata
     8401 gtcttttaaa atcagaggag gcagaagctt ctgtcactag aaaatatgac aaatgtcata
     8461 aaaaattctg ttcaaaacaa gcaagataca ctatacaata aaacacaatc agaaaaatct
     8521 aaggcaactt cctcaaaaga gatatcaaac tcaattcaca ccataatgta aactaatact
     8581 tatttaaaat caaaaagagt
//
LOCUS       DAVLYR010000080         8591 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLYR010000080 DAVLYR010000000
VERSION     DAVLYR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.080"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(34..684)
                     /locus_tag="VYO63_001346"
     CDS             complement(34..684)
                     /locus_tag="VYO63_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1672257.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            796..1638
                     /locus_tag="VYO63_001347"
     CDS             796..1638
                     /locus_tag="VYO63_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1672258.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            1714..2151
                     /locus_tag="VYO63_001348"
     CDS             1714..2151
                     /locus_tag="VYO63_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1672259.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            2220..2522
                     /locus_tag="VYO63_001349"
     CDS             2220..2522
                     /locus_tag="VYO63_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672260.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(2613..3686)
                     /locus_tag="VYO63_001350"
     CDS             complement(2613..3686)
                     /locus_tag="VYO63_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1672261.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            3842..4519
                     /locus_tag="VYO63_001351"
     CDS             3842..4519
                     /locus_tag="VYO63_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672262.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            4512..5486
                     /locus_tag="VYO63_001352"
     CDS             4512..5486
                     /locus_tag="VYO63_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1672263.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(5535..7478)
                     /gene="thrS"
                     /locus_tag="VYO63_001353"
     CDS             complement(5535..7478)
                     /gene="thrS"
                     /locus_tag="VYO63_001353"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1672264.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(7536..7859)
                     /locus_tag="VYO63_001354"
     CDS             complement(7536..7859)
                     /locus_tag="VYO63_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1672265.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
BASE COUNT         2379 a         1592 c         1992 g         2628 t
ORIGIN      
        1 aaaactttca gggttggtag gggacttgag aaattagttg attttctcga catagagttg
       61 ttttgcaatg tccatattca cttgtaaatc ctcgtcgtta ctgaggatag tgaaggtatt
      121 gctgaagcca tcaaactgct taacttggag ctggtcaccg atgtgaagtg agtgcttgtc
      181 cagataatgg agaatgtcaa aactatcgtg catccgagtc aggcggtagg cgccagcttc
      241 cttgatatca gctagtggga ggttattgat ttcaacgagg agttctccct tggcaggaat
      301 agttcctccg tgggggcagg ttttagggaa acctagcagt ttatctagtc tttccacgaa
      361 caggtcagag acagtgtgtt ccaagacctc agcttcctcg tgaatctggt cacttgtata
      421 gtctaaatga tgaactagaa aaacttcaat caagcggtgc ttacgataga gctcagagac
      481 cagtttgaga ccgaggtcag tcagtagata gccacattcc ttgtccttta ggatgagatt
      541 ttcacttttc attcgtttga tcatttcagt tacggcaggg ggagagactt gcatgcgagc
      601 cgcaatttcc ttgttggtaa tcttatgcag gtctatgcca atttcataaa tacattttag
      661 atagtcttct ttgtttgggg tcattcgttt cctcttgtct aatatcttta tctagtatat
      721 caaaaaaagc tagatttctc tagctgtgac tttttatgtt atacttattg caagagaaaa
      781 aacgaggaga tggatatgac gaaaatagct cttctttcag atattcatgg aaataccacc
      841 gccttggagg ctgttttggc agatgctcgg cagctaggag tggatgaata ctggcttttg
      901 ggagatattc tcatgccagg gacagggcgt agaaggattt tggacttgtt ggatcaacta
      961 ccgattacgg ctagagtttt gggaaactgg gaagacagtc tttggcatgg tgtccgtaag
     1021 gaattggata gtactcgccc tagtcaacgc tatctcttgc gccagtgcca gtatgtttta
     1081 gaggaaattt ccctagaaga aattgaagtg ctccacaatc aacctcttca aattcatcgt
     1141 cagtttgggg atttgacagt gggaattagc catcatctgc ctgataagaa ctgggggcga
     1201 gagttgattc atactggcaa acaagaggag tttgaccgct tggtgactca tcctccctgt
     1261 gatattgctg tttatggaca cattcaccag cagttgcttc gttacgggac tggtgggcaa
     1321 ttgattgtca atccgggttc gattggccaa cctttctttt tggacgctca gctacggaag
     1381 gacttgcggg cccagtatat gattttggag tttgatgaca agggcctggt agatatggac
     1441 ttccgacggg tagattacga tgtggcagct gaattgcagc tggctaaaga cctgagactt
     1501 ccctattttg aggtttacta tgaaagtctg gtcaatggga tccaccatac tcatcatcag
     1561 gaatttttga gagaattggc tcagaaagag ggttgcgatc gggagttaga cgactggttg
     1621 aaaagtggta acgattgaca ttacaactaa aaagggtata ataatagaaa aagaagagta
     1681 gaggcagggt agcctcgtaa aaaggagaag aggatgcaaa ttccaagtag atttaccatt
     1741 gcaactcata tgctgataat cattgccctc gaggggaagg aaagcaaggt gaccagtgat
     1801 tttctggctg ctagtgttgg ggtcaatcct gtcattatca gaaagatctt gtcccagttg
     1861 aagaaggcag agctgatttc agtagcgcgt ggaacgggcg gaacagagat tgtcaaggac
     1921 cttaaggata ttagtctttt agatgtttat caggcggtcg aatgtcttgg taagacaggt
     1981 caactcttca gtttccatga caatccgaat ccaaattgcc ctgtaggagc tcatattcat
     2041 gatgttttgg atcaaaaatt ggagagaatt cagttgacta tggaggcaga acttggtcag
     2101 accagtctag aaaaagtcgt ggccgatgca gagagtcaga tgaaggatta agagggataa
     2161 atgccctctt tttttctttg gataattatg ataaaatgta gctattaaag gagtaagaaa
     2221 tgtatctcgt tatagaaatt gtattagcct ttatagtgtc attttggaag gataatagaa
     2281 gtttgtggaa tccagtatta tttttattat ccctcatttc aagttatttt tatctatcct
     2341 acctttttta taaaaatgga tacgagaatg ttcagttagc tttttatgta tttgcctttg
     2401 tcttacttcc ttttttgctt tttctaagtg gcatcttttt aatctataat ggggtcattt
     2461 tgttgaaacg agagggacgt tccaagcccc attatctctc aatgctcttt gatttttatt
     2521 gactattaga ttctatttaa aaagacttag aatgaacatg aaagtatgta ttctaggtct
     2581 tttgttttgt atacatttaa ttttcaagag tattatctat ttttccatgc atggtaacga
     2641 gtatcaatca ataactgggt aaggcgaccc aaggtttctc tttcttctgg agtgagggat
     2701 tgagcttgga caaagagatg atgaatgtat tcaagcgcct ttaaggaagc caagtcattg
     2761 atagagctaa taccagcatc aagaatagtg ccaaagaaga ggtcgaagta gagctgaagt
     2821 tcttcgtcag ggactgtggc cacccattct ttaaaggttg tgtctacttg ccggctatca
     2881 ctgttggtct tatccagttg aacgaagtgc ttgtcctcaa tctgccaact aaaggtatcg
     2941 tgctgggcga tgccacccag ggcagtactc tgaacgatga tttggtgagc aggaatttcc
     3001 agcatcatac cgataatgga accttgtgga atgaatatct tgcttctatc cattatcctt
     3061 tgataacccg cagtctgtgt caattcttga tggagaccag gtgcatcaaa tgtataaact
     3121 gctgtgatct gattttgcaa actttgctca atttggctag cagcatagat agcgagattt
     3181 cctcccttgg aatgcccagc cagaataacc ttttgcttag gatgatgggc aaaaaagttc
     3241 tttaaatagc gaagggcgtg cttttgagca ggaatttcct tcatataggt caggtggaaa
     3301 tcttccttcc agccaatgat actgtcatct gtcccacgaa agacaatcag ataggtatcg
     3361 aggctgacac gataagtcat agccgcaaat tgcttttgca gttcagggtc gatgtcgttg
     3421 ataaaatggg agagtttgca atttttgaag cgcttgtgtt gagccaattc atctaataat
     3481 tgaaggcgat ttttgctagt aagcatggtg ggctctcttg gaacctgagg tgctagatct
     3541 aaaagacgtt gaggaagtgt ggagaccaga ttatcaaagg agaggtaggt gatttctatt
     3601 aaggttagaa tgtctaactc attcaaggga aggtcgtaat aagaatcata tgcgacatct
     3661 ttcagatagt caaaaatatt ggccataaga gctcctttct ttttatttat cttagcaaat
     3721 ttccctcaaa ttagctagta gcatagcctg tttgtactgg ctaaaaacag gctatttcaa
     3781 attcagtttc agaccatcta gcatggaaaa atctgttata ataatggaaa aggagaagcg
     3841 catgcacaag attttattaa tagaagatga tcaggtcatt cgtcaacaga ttgggaaaat
     3901 gctctctgaa tggggatttg aagtggtcct ggtagaagac tttatggaag ttttgagtct
     3961 atttgttcag tcggaacctc atctggtcct catggatatt ggtttgccct tgtttaatgg
     4021 ttatcactgg tgtcaggaaa tccgcaagat ttccaaggta cctatcatgt ttctttcttc
     4081 gagagaccag gctatggata ttgtcatggc aatcaatatg ggggcggatg actttgtgac
     4141 caagcctttt gaccagcagg ttcttttagc taaggttcag ggcttgttgc gtcgttccta
     4201 tgagtttggg cgtgatgaga gtttgctgga atatgctggt gttatcctca ataccaaatc
     4261 catggattta cattatcaag ggcaagtctt gaatttgacc aagaatgaat tccagatttt
     4321 acgcgtgtta tttgagcatg caggcaacat cgtagcacgt gacgacctga tgcgggaact
     4381 ttggaacagt gactttttca ttgatgataa taccctctct gtcaatgtgg ctcgtttgcg
     4441 taaaaagttg gaggagcagg gattggtagg atttatcgag accaagaaag gaatagggta
     4501 cggattgaag catgcttgat tggaaacaat tttttctagc ctatctgcgc tcccgtagtc
     4561 gtctttttat ctatctgctt tctttggcat ttcttgtctt actctttcag tttttatttg
     4621 ccagtctagg aatttacttc ctctactttt tcttcttgtg ttgctttgta accatattat
     4681 ttttcacttg ggacatattg gtggaaacgc aggtctatcg ccaggaactt ctctatggag
     4741 agagggaagc caagtctcct ttggaaatag ctttagcaga aaaattagaa gcgcgtgaga
     4801 tggaactcta tcagcagagg tcaaaagcag aaagaaaact gacggatttg ctggattact
     4861 ataccttgtg ggtccatcag ataaagaccc ccattgcagc cagtcaactc ttagttgcag
     4921 aagtggtcga ccgccaactg aagcagcaac tagaacagga aattttcaaa atcgactcct
     4981 ataccaacct agttttacag tacctgcgtt tagaaagttt ccatgatgat ttggtcttaa
     5041 agcaggttca aattgaggac ttggtcaagg aaataattcg taaatatgct cttttcttta
     5101 ttcaaaaagg cttaaatgtc aatctacatg accttgataa agaaatcgtg acggataaaa
     5161 agtggctgct agtggttatt gagcaaatca tctcaaacag tctcaagtac accaaggaag
     5221 gtggtctgga gatttatatg gatgaccaag agctttgtat caaagatacg ggaatcggga
     5281 taaaaaacag tgatgtcctc cgagtatttg aacgtggctt ttcaggatac aatggccgtt
     5341 tgacccagca gtcctctgga cttggccttt atctatctaa gaaaatttct gaagaactgg
     5401 ggcaccagat tcgtatcgag tctgaggtcg gaaaaggaac gacagtgcgg attcagtttg
     5461 ctcaagtgaa cttagtcctt gagtaaaaat agatgagaaa aagcctccag attggaggct
     5521 tttatgctag actcttattt ctcaacgcgt gatttgttgg cgatatcagc taggatagct
     5581 tgaacaaaat tatcaactga gacagtttgt gtttcttttt ggccgtagcg acgaacgttg
     5641 actgtttcgt cttccatttc tttgtctcca acaattaatt ggtaaggaat cttgctggtt
     5701 tgtgaagcac gaatcttgaa ctgcattttt tcattgcgct catctacgtc tgcacggaca
     5761 ccgcggtcac ggagtttctt ggccacttcc caagcgtagt ccacgtgttt ttcgttagat
     5821 actgggatga gggttacttg gtgtggtgcc agccatgttg ggaaggcccc cttgtagttc
     5881 tcaatcaaga tagctgtgaa gcgttccata gttgagataa ccccacggtg gatcatgact
     5941 ggacggtgat cttcgccatc agctccgatg tatttgaggt cgaagcgttc tggcaacaag
     6001 aaatcaagtt ggatagtaga aagggtttct tcttttccaa gggcagtttt aatctggata
     6061 tccaattttg gtccgtagaa ggctgcttca ccttcggctt caaagtagtc cacgcccatt
     6121 tcatcaagag ctgcacgaag catggtttgg gcattttccc acatctcatc gttatcaaag
     6181 tacttatgag tatcttgagg gtcacgaaga gagaggcgga agcggtagtc agtcaagttg
     6241 aagtcttcat aaacatcgat aatcaactga agggcacgtt ggaattcttc ttggatttgt
     6301 tctggagtaa cgaataggtg accgtcgttg agtgacattt cacgtacacg ttgaaggcca
     6361 gtgagggcac cagatttttc gtaacggtgc atcataccga tttcagcgat acggattggc
     6421 aattcacggt aagagtgaac atggtgtttg aaaacttgga tgtggtgcgg acagttcatt
     6481 ggacgaagga caaattcttc cccgtcaccc atgtccatgg ttgggaacat gtcttcttgg
     6541 taatgatccc agtgaccaga agtcttgtaa agctcaacag aagcaagtgg tggagtgtag
     6601 acgtgttggt agccagaagc caactctttg tttacgatgt agcgttccaa ttcacgacgg
     6661 atagtcgcac catttggcaa ccagaatggc aaaccttgtc ccacttcttg tgaaatcata
     6721 aagaggtcaa gctctttacc aagtttacgg tggtcacgtt ccttagcttc ttcacgcatt
     6781 tgaaggtagt ttttcaagtc tttcttgtca aaccaagctg taccgtagat acgttgcatc
     6841 atagcgttgt cgctgtttcc acgccagtac gcaccagcta catggagaag gtggaagatt
     6901 tggatacgac ctgttgatgg aacgtgaggt ccacggcaga ggtctacata ttcaccctga
     6961 cgatagatag tcaaaccgcc ttcgtcttct gagtgttctt caatcaattc caacttgtaa
     7021 gggtcatttt tgaagatttc acgtgcctcg tctttagtca cttcttcacg aatagatggg
     7081 aagttttctt tgacgatttt ttgcatttct tcttcgatac gaggaaggtc ttcgttagag
     7141 atttgaccag ctgtgttgtc agtatcgtag tagaaaccat cttcgatggc tggaccaact
     7201 cccaagtgaa tgtctgggaa aagacgacga gctgcttggg cgaacaagtg agctgctgag
     7261 tgacgcaaga ttggaagggc atcttcgtga tcaggtgtca caatttcgat gcttccatct
     7321 tcagtgatag cgcgagtagt gtcgatgagt ttgccgttga atttaccagc caaggctttt
     7381 ttagctaggg aattgctgat agattgggca atttcaaaag ttgttacgcc agattcgaat
     7441 tcacgaacag cgccatctgg gaaagtaatg ttaatcatga tgttctcctt ttttattatt
     7501 tcatagtttc taagcattga ttttttatca gcagactact tccaagtcct aacgttcaat
     7561 tgcttttctt tatcagagag gaagttttcc atttccgtca gaatgtctat atccaaacgt
     7621 tgagaaagtt ctaatagcca ccagatattt tctgaaagtt tttgttccag tgtgtagggt
     7681 gtttcatcat agtagcgtcc ttgctttgtc atcaccagtc gttggaaatt tccaatatca
     7741 ttagataaag ccaagaggtc ttcttctacc gtccacttga aatcatgatg cttaacttcc
     7801 agttcgtgat aagcttgtcg gattgcccaa ctgcgctcca ctaattcttg caactccata
     7861 attagtatcc taataaaaaa atgcgacatc ctcgaaagga cgtcgcaacg tggttccacc
     7921 ttcatttatg tacctcaaaa gagagggaca cctctgatcg gctctaacgt ggccaccgtt
     7981 ttatgttttc ataaaaaact caagagtagt atcagtttag acttcctatg cgtttccagc
     8041 aaccacgcac tctctagtag aaagggacta agtgacttgt ctctatggat tattatagca
     8101 tgattgtgag atttttcaag gattttgtga aagttttttg tttttcatat tttgtagaat
     8161 atgcttgatg ctaataccag aatccatgcc caccagagca actgtttcat agattagcaa
     8221 gtaaattagt ataaactcac caaaggttat gatagtaaag taaaagagca gtccgttacc
     8281 aactaaccat caaagtgaga aacggaaacg gtagctatag agtaacgata caatcaagat
     8341 taccagaggt gtaaagaaga gaagattgtt tatatagtgt aaaggggtca gaaacaacta
     8401 gcaagaacaa ctcataaagt ggccaataaa agaataatat gaccagatag taggggagat
     8461 gggaatagaa tctacgcatg ataatcacct cgttttaaaa aaacagacaa cttagatctc
     8521 ttgctatgat tattatagca tgtttaaaac aagaaagtaa atttgttgac aaagaagatt
     8581 tttcttttca c
//
LOCUS       DAVLYR010000081         8474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLYR010000081 DAVLYR010000000
VERSION     DAVLYR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.081"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(74..727)
                     /gene="phoU"
                     /locus_tag="VYO63_001355"
     CDS             complement(74..727)
                     /gene="phoU"
                     /locus_tag="VYO63_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1672266.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(739..1497)
                     /gene="pstB"
                     /locus_tag="VYO63_001356"
     CDS             complement(739..1497)
                     /gene="pstB"
                     /locus_tag="VYO63_001356"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1672267.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            complement(1510..2313)
                     /gene="pstB"
                     /locus_tag="VYO63_001357"
     CDS             complement(1510..2313)
                     /gene="pstB"
                     /locus_tag="VYO63_001357"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1672268.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2324..3208)
                     /gene="pstA"
                     /locus_tag="VYO63_001358"
     CDS             complement(2324..3208)
                     /gene="pstA"
                     /locus_tag="VYO63_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1672269.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3198..4115)
                     /gene="pstC"
                     /locus_tag="VYO63_001359"
     CDS             complement(3198..4115)
                     /gene="pstC"
                     /locus_tag="VYO63_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1672270.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4115..4993)
                     /gene="pstS"
                     /locus_tag="VYO63_001360"
     CDS             complement(4115..4993)
                     /gene="pstS"
                     /locus_tag="VYO63_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1672271.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5567..6871)
                     /locus_tag="VYO63_001361"
     CDS             complement(5567..6871)
                     /locus_tag="VYO63_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1672272.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6872..7645)
                     /locus_tag="VYO63_001362"
     CDS             complement(6872..7645)
                     /locus_tag="VYO63_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1672273.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7635..7913)
                     /locus_tag="VYO63_001363"
     CDS             complement(7635..7913)
                     /locus_tag="VYO63_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1672274.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7913..8314)
                     /locus_tag="VYO63_001364"
     CDS             complement(7913..8314)
                     /locus_tag="VYO63_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1672275.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT         2521 a         1988 c         1588 g         2377 t
ORIGIN      
        1 aaaatatttt ttcgttcaaa aaaaggagcc atcagttgat ttcaagctcc tttttataca
       61 gaattaaact attttatagt tcgacaatct tacctgtttc aaagtagaca acccattcac
      121 agatattttt agcatagtca ccgatacgct ccaagtagga aataacttgg aaataatcac
      181 gacccgtaac aatggcttct ggatttttct taatctcttc agtcgcaagg tcacggatag
      241 tttcaaaata gtggttaatt tgctcatcca tggaggccac ccggtatgcg tcgtcaacag
      301 aaccattaag ataaagatca agtgctgctt caacaacgct tttaacttca cgtcccattt
      361 ttttaatttc ttcctctaca gctggaatgc gctcttcccc cttcatacgg atggttgcct
      421 gagcaatggc tacagcgtga tcccccatac gctccacatc tgatacagcc ttaaggacag
      481 tcaagactgt acgcaaatct tgagagactg gttgttggag tgcgatcatt tcaaatgatt
      541 tcttttccag tttcacttcg tattcattta cttctgcatc atcttcgatg acctcttttg
      601 ccaggtcacg gtcatgcgtg acaaaagcac gtaccgtacg attgatttgt gagagcactt
      661 cttgtcccat agcgtagaac tggttatgta atttctctaa atcttcttca aattgagatc
      721 gtaacatctt tcatctcctt atccaaattt tcctgtaata tagtcttccg tttccttgtg
      781 ttggggatta aggaacatct gcttggtatc attaaattca atcaaatctc catctaggaa
      841 aaatcctgtc ttatcagaga tacgtgaagc ttgctgcatg gaacgggtta ccagaagcat
      901 ggtgtacttg tcttttagac catacaaggt ttcctcaatt ttaccagctg aaatcggatc
      961 caaagccgaa gttggctcat ccaagaggat gattttagga ctagttgcca agacacgggc
     1021 cacgcagaca cgctgctgtt gaccacctga caatccaata gctgaatcat atagacgatc
     1081 cttgacctca tcccagatag aggcaccttg caaggctttt tctacggctt catccagaac
     1141 ctgcttatcc ttaattccat tgatacgaag cccgtagaca acattctcat agatagtcat
     1201 agggaaagga ttaggttgtt ggaaaaccat tccgatttcc ttacgtaatt caaccgtatc
     1261 tgtacgcgga ctgtagatgt tgtgaccatt gtacaccacg gatccagttg tggtcacctc
     1321 tggattgaga tctcccatgc ggttgagaga cttgaggagg gttgacttcc ctgatccaga
     1381 tggaccaatc aaggctgtaa tttccttagg ttggaaagat agggaaacac tattcaaagc
     1441 cttcttttta ttataataaa cggacaggtc tgatacctgt aaaatcgcat ctgtcatacg
     1501 gtttcctttc taaccaaagt gaccagatac atagtcattg gtggactgta gcttggcatt
     1561 ttggaaaata gttgcagtct tgtcatactc aatcaaatca cccaagtaaa agaagcctgt
     1621 atagtcactt gcacgagcag cctgctgcat attatgcgtt acaatgatga tggtaaagtt
     1681 tttcttgagc tcaaacatgg tctcttctag ttgcatggtc gcaatcggat ccaaggctga
     1741 ggctggctca tccattaaga ggatatctgg cttaacagag atggcacgag cgatacagag
     1801 acgttgttgc tgaccacctg ataaggtcaa ggctgacttg tggagatcgt ctttaacctg
     1861 atcccaaagg gcagcctgac gaagggaggt ttctacgatt tcatctagga cttgcttatc
     1921 cttaactcca gcacgttcat gcgcaaaggt aatattacgg taaattgact tagcaaatgg
     1981 attggggcgt tgaaaaacca ttccaatgtg tttacgcatt tcataaacgt tgatttctgg
     2041 acggttgaca tcaattccac gatagagaat ctgcccagtt actttagcaa tatcaatggt
     2101 atcattcatg cgattgagac tgcgtaagta ggtagatttc cccgatcccg acggaccaat
     2161 caaagctgta attttatttc tttcaaattg catatcaatc cccttaatgg attcattttt
     2221 accatagtaa acatggacat ccttagtaga aagggctact ttttcttcag gaaaggtaag
     2281 gatatgcttc tcatcccagt tatatgttga catggcttct cctttaggca gcggttaatt
     2341 tcttgtgtag atagcttccg aacttacgag ctccaaagtt aaaaatcagg ataaagatca
     2401 ggagcacagc ggcagaacct gctgatacaa tggttccatc tggaatagtg ccttcactat
     2461 tgactttcca gatatggaca gccaaggttt ctgcttgacg gaagatagag atggggctag
     2521 tcacactgag gatattccag ttagaccagt caagagctgg cgccgattgc cctgctgtat
     2581 agatcagagc tgcagcttcg ccaaagatac gaccagatgc caaaacgaca cccgttacaa
     2641 tacctggaag cgcttctgga ataacaacat gaaccactgt ctcccagcga gaaatcccaa
     2701 gagccagacc agcctcacgt tgggtatggt gaacgtgttt caaactatcc tctacattac
     2761 gcgtcatctg aggcaagtta aagactgtca aggccaaggc acctgaaatg attgaaaatc
     2821 catactcaaa ctggactaca aagatcaagt aaccaaagag acccaccacc actgatggta
     2881 aagaggacaa gatttcaata caagtccgca caaagttggt aacaggacct tttttagcat
     2941 attcagccaa gtaaatccca gctcccatag aaagaggtac agaaataatc aaggtaatga
     3001 ccaataggaa aaaggaattg taaagctgaa tgccaatccc accacctgct tgaaaagcag
     3061 aagaccttcc agtcaagaaa gaccaagaga tatggggcaa gccccgaacc aagatataga
     3121 gaatcaagga agccaagatt gtcacaatga tgctagcaat cgtatagagg acagctgttg
     3181 caagtttatc taatttctta gcgcgcataa tttttctttc ctctttcttt cgtaatcaat
     3241 ttaatcacac tgttaaaaac taaactcatc aagagcagta ccaaggccag tgaccagaga
     3301 acattattat ttacagttcc catgacagtg ttcccaattc ccatagttaa tatagaagtt
     3361 aaagttgcag ctggtgtggt caaggaagtt gggataacag ctgagtttcc gacaaccatc
     3421 tggatagcta gagcctcacc aaaggcacgc gccatcccaa agaccactgc agtgaaaata
     3481 ccagaacggg ccgccttcaa gatcacacgc cagatagtct gccagcgagt ggctcccata
     3541 gcgaaactgg cttcacgata ataacgagga accgcacgca agctatccgt tgtcataaag
     3601 gttacggtcg gcaaaatcat gacaaagagg acggaaatcc ctgacaaaat cccaaaacca
     3661 gtcccaccaa agacactgcg aacaaaggga acgacgactt gcaagccaat aaatccgtac
     3721 actactgaag gaatcccaac caggagttca atagctggtt gcaaaatctt cgcccctttt
     3781 ggtgatactt cggtcataaa aactgctgca ccaatagcaa agggtgttgc gataagggct
     3841 gagagaatgg taacgataaa ggaacccaaa atcataggaa gggcaccaaa ttctttacta
     3901 gaaggattcc aagttcctcc caaaagaaag tcaaagatat tcacaccatt gacaaagaag
     3961 gtcgacaagc ctttttgcgc tacgaaaacc aaaatcatgg ccacaaggat gactatcaaa
     4021 gaaagacagg caaaggtcaa accttttcct aatttctcca gacgagaatt ctttgatgga
     4081 agcaacattt tcttagctaa ttcttcttga ttcattattg tctcccttcc aacactgtca
     4141 cagttccggc agcatctttt tcaaccttca tttccttaat cggaatatac ttcaatcctt
     4201 tgacaatccc ttcttgggtc tcatccgaga gaacaaaatt gagaaattct gcagccaact
     4261 cattgggctg ccccaatgta tacatatgct cataagacca caagggccaa ttattgctac
     4321 ttatattttc tggacttaag tcatagccat tcaacttcat gcttttgacc gaatcatcta
     4381 tataggtaag agataaataa gagatagctc ctggactttt tgatacgatt gattttaccg
     4441 ctccatttga atcctgctcc tgactttgca tggcagactg accttccata atgacagtat
     4501 caaaggtagc acgagagcca gagccggctg cccgattgat aacagagatg ggtaagtcct
     4561 taccaccaac ctctttccaa ttggttacct cacctatgaa gatttgacga agttgctctg
     4621 tcgttaggtt atcaacatca acctccttat tgacaatcag agccaagcca gctaccgcga
     4681 ccttgtggtc aacaagagca gaagcatcaa ttccgtcttt ttcctcagca aatacatctg
     4741 agtttcctat atcaactgcc ccagactgaa cctgggacaa gcctgtacca gaacctcccc
     4801 cttggacatt gaccgttttt ccaacatgga tcgtgccaaa ttcatctgcc gctacttcaa
     4861 ccaagggttg caaggcagtt gagccaacag ccgttatgga ttctccacga tcaatccagc
     4921 tagcacagcc tactaaacaa gccgtcagcc aaaaagcgat aagagacaga gcaagctttt
     4981 ttcttttttt cactgttttt ctcctcgaaa ataattatga atactgtgaa ttttttaagt
     5041 agttctttat gagttgacgc atgaattctt accaaatttc tgcgcaattg attatttata
     5101 taatataggc tatattactc tttcctaacc tccttttttc atatgtggat aaaatctctt
     5161 gtctatccct tcccccattg tcacccatta tagtcatttc gtgtctcttt ttcccctttt
     5221 taatgcaagg gaaattactc ttcttagatg ataatccaaa agctagaaag gtatctcaaa
     5281 cctctctact ctcccagact agtttacaac taaaaggaaa agattctatt ttatgagaaa
     5341 tctagtttac aagcggtaag aacgctaata actaaacttc ttgtactctt tgaaaatctc
     5401 ttcaaagcag tgttttgagc tatctatggc tagcttccta gtttgctctt tgattttcat
     5461 cgagtagtaa aactacatgt gatggcaatc aagatatcaa gaatcatcct actaaaaaaa
     5521 tccatacttt cactataaca tagaataaga tatttgacta gcattttcat ttgaatctga
     5581 ggccttttgg aaaataattt ttcaaaacat ttccagtaac ctttgcaaag cccaagccat
     5641 tgcctttaac caaaacttgg taccaaccat ttggcagact ttctgccagc tgaacggttt
     5701 ctccagccgc atacttgaca aacgcttctt ggccaatttc aaccgactgt tcgacctgac
     5761 tcggtttcaa ggctaaacca agagcgaaac tgggctcaaa gcgtttcttc ttaaaagtac
     5821 ccagatgcag tccattgcga gcaatcttga gcttccctaa atctggcaaa agttctggca
     5881 agagataaag ctggtctcca aaaatctgca agatacccgg tagattgacc ttcaaatggt
     5941 tttgggcaaa ttcctgccac aaggcaactt gttcacggct gaggttactc ttacttgcct
     6001 taaatttagg agctggattg ttacccttaa actgtagatg ggcaacaaac tgaccctctc
     6061 ccttaaactg atgagggtac atccgagccg tttctggcag gtcaatacca gctaccattc
     6121 cattgatatg ctctactggc aacaagtcaa aatcatactc ttccagcaac caattgacaa
     6181 tctcttcgtt ttcctcgggt gcccaggtac aggtcgaata aaccagacga ccaccttcag
     6241 ctaacatggt cactgcatcc tccagaattt ctctttgcaa gctagcacat tgactcggat
     6301 aatctaagct ccaatagtcc atagcatcag gttgcttacg aaacattcct tcaccagagc
     6361 aaggggcatc aagaacgatt aagtcaaaat agcctttaaa gaccttgacc aagcggtcgg
     6421 cagattcatt ggtcaccacg acatttgtcg ctccaaaacg ctccatgttt tcaaccaaaa
     6481 tcttagcccg tttgcttgaa atttcattgg aaacaagtag cccctcccct gctagatagg
     6541 ctgccagttg agttgatttg ccccccggtg cagcagccaa gtccaagacc ttcataccag
     6601 gactgggttg ggctacttga gccaccattt gagcagcagg ttcttgcgaa taaactaaac
     6661 ctgtagcatg ctcaggcgat ttccctgaaa ccttcccata gtggccccaa ggggtttgag
     6721 taatggcatc agaaaaggaa agttgctctt cttttaaggg attgacccga aaggccgaaa
     6781 ccgcttcctc ctcaaaagag gcaagaaaat ctcttgcctc atctcctagt atttctttat
     6841 atttttcaac aaatccttct ggaaatttca tttaagttct tttcctttcg taaatatagg
     6901 actgaatttc ctcctgcatc tcaagaggca ccatcatgac cggctgtctg gtttgaaaat
     6961 caggagcttc accaaaaagg gtcacaaccc gatagcccag actttcccct aaaatactag
     7021 ctgcggcata atcccatggt tgcagatagg tgagataggt caacaaatgc cctgacaaaa
     7081 tcttggcaaa actaatggcc gcacttccat agacacgaac accaagaacc gctcggctca
     7141 aatcagccag cccccattca ttggtttcca gcataccact attccctgca atgagaaaat
     7201 ctccaagtgg tttagtttta aaaggagcta ggggcctatc atttagacaa actggaaatt
     7261 ccccaccacc gtggtaacaa tcccctttga ccacatcata aatcagacca aactgtccct
     7321 gaccattttc aaaataagcc atcataacag caaaatcttc ctgctgggct acaaaattat
     7381 tggtaccatc aatgggatca atgacccaaa ccttgccctc ttgaaccgag gctcgcagac
     7441 aaccttcttc agcacaaatc ttatcctcag gataacggga caaaatctca ccaaccaaga
     7501 gttcctgaac ttctttgtcc agtctggtca ccaaatctgt tggagaggac ttggtttcaa
     7561 cacgcaagtc ttcctgcata tggtcaagaa tgtactggcc tgctttctta acaagctctt
     7621 tagcaaattc aaatttactt tccaagagaa atctttcctt cccctttttc tttggcagcc
     7681 tgaactgctc ggtaaagtga atagccacta accgtttcaa attctcgtcc caaacgtttc
     7741 tcttcgctct tgcttggcac gaccgctttg aatcccttat aggagtccag taacttttta
     7801 gcctctactt tgccttcata ggcagcttca acatcattaa aaaaagaaag cactgaagca
     7861 agttcttcag tgctccacga caaatctagt gggtaactat actgtttgtt cattaactaa
     7921 taccagctct cattcttgct tcttttagtt cttgcttacg ataactacga gggagaaaag
     7981 cacgaatctc atcttcatta aaaccgattt gcatacgctt ggcatcaata ataattggac
     8041 gacgcaaaag actaggatac tgctcaatca aatgaagcaa ttccgatacc gaaatactct
     8101 ctacatcaat attcaatttt tgaaaaattt ttgaacgagt tgaaatgatg tcatcagtac
     8161 cattttcggt caaggaaagg atgtgttgca attcttttct tgttaaagga ctggtcataa
     8221 tattgtgttc cacaaaggga accttatgtt tttctaacca ggccttagcc ttacgacatg
     8281 atgtacagct cggtgataga aatagtgtaa tcatgctttt ctcttcttat ctatactttg
     8341 ctacttctat tatacaaaaa aataaagcgc ttgactaggg atttttagaa aaaaagccta
     8401 ttttttcaag aaaaataggc tttttgcgaa cgattgacac aattggattt ggttaattca
     8461 ctcttaacga tggt
//
LOCUS       DAVLYR010000082         8384 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLYR010000082 DAVLYR010000000
VERSION     DAVLYR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.082"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(162..365)
                     /locus_tag="VYO63_001365"
     CDS             complement(162..365)
                     /locus_tag="VYO63_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672276.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(358..1440)
                     /locus_tag="VYO63_001366"
     CDS             complement(358..1440)
                     /locus_tag="VYO63_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1672277.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTIFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     IIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1566..2348)
                     /locus_tag="VYO63_001367"
     CDS             complement(1566..2348)
                     /locus_tag="VYO63_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1672278.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2348..3691)
                     /locus_tag="VYO63_001368"
     CDS             complement(2348..3691)
                     /locus_tag="VYO63_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1672279.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3803..5119)
                     /locus_tag="VYO63_001369"
     CDS             complement(3803..5119)
                     /locus_tag="VYO63_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1672280.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5313..6539
                     /locus_tag="VYO63_001370"
     CDS             5313..6539
                     /locus_tag="VYO63_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1672281.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGILGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6738..8312
                     /locus_tag="VYO63_001371"
     CDS             6738..8312
                     /locus_tag="VYO63_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1672282.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2392 a         1631 c         1864 g         2497 t
ORIGIN      
        1 ccctgtgcaa tacagaacta aatccttcgg ataaattaat tgtctaactt tttggggggc
       61 agttcattct acacccacta ttatgcgttt tattgtggga agattttcct cattttctag
      121 tgaaattgaa ctttttctaa catcttttga tagatgactt cttaatcaat tatcatttcc
      181 attgttgttt cttggggttt catcccttgt ctttgataaa agcggagagc accctcgtta
      241 tcattccaga cacttagggt tagattattg cagccaattt ctttagcata atagcagaaa
      301 aatgacaata aaatagcccc tagatatttt gaaaggaaag cataggggct atatagttta
      361 atcaaaataa agcaaatctt tgctggtgct tgtaaagagg tcgaagccat ccttggtaac
      421 aacaccgcag tcttcaatac gaacaccgac tttaccaggg atatagatac ctggttcaac
      481 agagaagcac atgccttctt cgatgaccat gtcgtttcct tccatgatag atgggaattc
      541 atggacatcc ataccgatac catgcccgag acggtggttg aagtactcac cataaccagc
      601 tttttcgatg atctcacggg cagcgcggtc cacttcatga gcagtcacac ctggcttgat
      661 aaagtcaaga gcagcttgtt gggcttcaag agtcaagttg taaatatctt tcttgaattg
      721 gtctggtttg ccgacagcga ctgtacgagt catatctgac gcatagccat tgaccagaac
      781 ccccaggtca aagaggagaa gagcatcatt ttcaacctta ttagctgctg gaatgccgtg
      841 tggattcgca gcattatcac cagtcaagac catggtatca aagctcattt cgtaaccttc
      901 acgtttcatg gcaaagtcaa tttgtgcgat gatatctgtc tcagtcttat caagagaaat
      961 attgtcaaaa ccaacatgaa cagccttgtc agcataaaga cctgcaacca tcattttttg
     1021 tacttcatca gctgatttga tgaggcgcat gcgttggata cgaggagtga ggttttcaaa
     1081 ctcagcagtt tcaaaaattg ttttcaaacc atggtatttg gtcaagatga gattgtcaaa
     1141 ctcaacagcg acacgtttga agtcaagttg tggaagagca tgtttgattt tttgccatgg
     1201 attttcagaa tcgacatagc ccactactgg gaaggaaacg gtgctacttg cacgttctac
     1261 ttcaagagct gggacaaaga ggagaggttc ctgatccgct aggacaaaga ggaacatttg
     1321 gcgttcatgg ggatcactgt aaaaaccagt gaggtaattg attgtgacgg ggtcagatac
     1381 gacagcgacg tctagttttt ctgattcaag atatgttagg atttgttgta atttagacat
     1441 atgctacctt ctttctaccc ctctattttg gcaaaaaatg agagaaaatg caagggagaa
     1501 gggtaaaaga aaagacaaaa aactccgaca agataacgga gttctttgat atcattttcc
     1561 tttgtttaag aaaagtcagc cttgcttttg acgtcactgt actcttcagc gatttcttgg
     1621 ctgaggatat cctcataggc agggagttgg atatcctgac catatttctt cttgagggca
     1681 gtagtaacta ggcgataagc cagattggca ttacgagaga ggataggccc gtggaagtag
     1741 gaaccaaaga cattcttata atgaacccct tcaccgacct tttcttcgtt gtttccattt
     1801 ccatagacaa cctgtcccag cggtttttgg tcatcagaga ggaaggtacg accttggtga
     1861 ttttcaaatc catagtaggt ttcatcgaaa tcttcattgt gaatcttgat gtcaccgata
     1921 aaacggttat tggtctggtt gagcgtgtag tgtcccatga cccctagccc ttcgatacgt
     1981 tttcctgaag cttcaacata atattgaccc aatagttgga aaccaccgca gatagccaga
     2041 actacaccgt cgttttggat gtagttgtca atgctctctt ttttagcagg taggtcgtct
     2101 gcaatgatac tttgttcaaa gtcttgacca ccaccgaaaa aggcgatgtc gtagtgattt
     2161 tcatcaaagt catcatggag agaaacgatg tcaacggtca catgggctcc cagtttttca
     2221 gccacatact tgagcatgag gatgtttcca ttgtccccgt aggtattcat gagatttccg
     2281 tagaggtggg caatgttgag ctgatagggg taattgccat cttttgagga aagtgaagta
     2341 taaaccatta gttcatctcc tttctaacaa tctgacgact agccagcagt tcacgaaatt
     2401 ccagcatggc agtataagtt gccagaatat aggcatgctt gcagtcttga ttctcaatgg
     2461 tcttgagaac ttgctccaga ttactcgttt cagtgatttt ctcagctgga tagccagtca
     2521 ctcggaggcg acgagcgatt tcagaatgac gaacaccgcc agcgttgatt tcaggaatgt
     2581 ccatgtcagt gatttgttca aagtctgcat cccagatcca gctagtgtca attccatctg
     2641 catagttggc attaaggagg acagataggc taaatggata aggtgctagt ttgatcattt
     2701 cgatagcttg ggttgcaccg actggatttt taatcaagac aagggtacat tccttgtcac
     2761 cgatatgaaa ggtttcttgg cgtccaaaga cagcacggct cttgtcaaat ccctgtttga
     2821 tgagttgcga atcggcacct aggaaacggg cgatggccac agcagctagg gcgttataga
     2881 tattatagag cccgccgatt tggataccgt attcttggcc gtctatgaca aagcgagagc
     2941 gattgttggt caactcaacc agttttgtca aacgatagtc gagatcagga cgtttacatc
     3001 cacaaccttc acagatatag gcacccaagt ttgcataggt attatgctca tatttgagga
     3061 tgccttggca gtcaggacag agaatccctt cggtattgta gtgagccagt tgggctggtc
     3121 ccttttccaa gtcaaaacca aaatactcta tagggtttgg aatagttggc ttgtagaaaa
     3181 gtggactgtc tccgttaagg agaacagtag cagttggaac tttccgaatg gcatccaata
     3241 tcatgttata ggtagtatag atttcaccga aacggtccat ctggtcacgg aagatattag
     3301 taatgacaaa aagactaggc tggatatagt cacagatacg agatagactg gcttcgtcaa
     3361 tttcgaggac ggcaatattt ttcccagttt tagaagattt ggctgttagg aaggttgttg
     3421 caatccctgt aatcatgttg gcaccgcttg ggttggttag aacttgacca taaacctctt
     3481 ttaaaatgcc gacagtgagg gcagttgtca gggtttttcc atttgttcca gtgacaacga
     3541 caatctcgta gttcttagct aggttttgta aaatatcttt atcaaattga agggcgactt
     3601 tccctgggag cgtacttcca cgtccaagac ggcttaaaac gaagtgggaa gaacgcccag
     3661 caagaaggcc caaagtagtt tttaagttca tgtttctatt atatcacaaa agtatatcac
     3721 aaaagaggga aaagacagat ttggtgtttc gtagaagtaa aaacagccca aagagggctg
     3781 tcagttaaga tgagatttca acttaaatcg caaacaagtc attcaagttc tcagccaagg
     3841 ttgggtgagt gaagatttgt tttgtgaagt aagtgtaagg aatcttgttg tccatagcaa
     3901 cagtaataat gttgatgatt tcttgagaac cttctgagaa gatgcttgca ccaagaattt
     3961 cttttgtttc agtattaaca acagctttga aagctccgcg aaggtctcca tttacgtgac
     4021 cacgaggcat ggctgcaaca gggatttctt tcactgcgta tggaagtttc aaatcagctg
     4081 cttggctttc agtcaaacca acttgtgaaa gtgcaggtgt gatgaacata gtatttggca
     4141 cattgagacg gtcctcaagt gtgtagctgc catctccagc aaggtagctg taaacaacac
     4201 ggaagtcatc aagtgaaatg taagtaaatt gaagaccacc gttgacatct ccaactgcaa
     4261 agacaccagg aacgtttgtt tgacagtgtt tatctacttt aatagcgcca cgttcagtta
     4321 gttcaatatc tgtattttca agttgaagtg gttctacatt tggtttgcgt ccagttgcgt
     4381 agagaagggc gtcgaaacgg taagtttcgt cttcagttac gacaagcact tggtcaccgt
     4441 cgtttttaat ttcagtagta tggatatttt gaagcaattc aataccgtct tcttccatgt
     4501 attgtttagc aagagctgcg atggaaggtt ctgcacgagg taggaatgta tccaaggcat
     4561 ctaggactgt aaccttgctt cctagtttat tgtaaaggcc agcaaattca agaccgatat
     4621 ttccgccacc aaggactcca agtttttcag gcaatttatc caagctttgg atacctgttg
     4681 agtcaaagac gtttttgctt gtagcaagtc cagggattgg caagacgttt gaaacagcac
     4741 cagtgttgat gacgatagtt tcagcagtca attcttgttt ttcatcacca gcttggattt
     4801 cgatgacttt atttgaaagg aagtgagctt cagcatcaaa gatatcgacg ccagtaccag
     4861 caacagtcgt atagttttta ccgttgaggc gaccagtgat cgtgtttttg gtagcaatga
     4921 cttcttcaaa agacaagtct ttctcagcag caaccagcaa agttttagtt gggatacaac
     4981 cgatgttgat acaagttcca ccgtacatag ctttgctacg ttcaacgagg gcaacttttt
     5041 tgccagctga agccaattta ccagctagtg ttttaccagc tttaccaaat ccgataacga
     5101 ttaaatcata agttaacatt tagaattcct cctattcgaa ttacattcta gcacaagaaa
     5161 aaaacttttg cacaattctg ctacgaaaaa gacagaaaaa tatgaaaacg cttttcaggc
     5221 aaaaaactag taattttttg acaagcttga taaactaaaa tcctatctat ttttatataa
     5281 gatagaataa atgttcgaaa ataggaggtt ttatgaaatc gaatcgttat attattgcct
     5341 ttgctggggt cattttacac ttaatgctgg gttcgactta tgcctggagt gtttatcgta
     5401 accctattat tgaaaaaacg ggatgggatc aggcttctgt tgccttcgcc tttagtctag
     5461 caatcttttg tttgggctta tcggctgcat ttatggggcg tttggtagaa aaatttggtc
     5521 cgagagtcat ggggagtcta tctgcttttc tatacgcagg tggaaatatc ttaacaggat
     5581 ttgcaataga ccgtcaggaa ctgtggttgt tgtatctagc ttatggcatt ttaggtgggc
     5641 ttggtttggg agcaggctat attacccctg tgtcaacgat tataaaatgg tttcctgata
     5701 aacgtggtct cgcaacaggt ttagcgatta tggggtttgg ttttgcttct ttattgacta
     5761 gtcccatagc gcaacatctc atcgcagggg tagggcttgt agaaactttt tatattttag
     5821 gtgcaagtta ctttattatc atgctcctag cttcacaatt cattaagcgt ccaaatgagc
     5881 aagagcttgc aattttatct gcttcaggga aagaaaaaac agactctctg acgcaaggga
     5941 tgactgcaaa tcaggctcta aaaagcaatc ggttttatat gctttggatt attttcttta
     6001 tcaacatagc ttgtggttta ggcttaattt cagcggcatc gccaatggca caggagatgg
     6061 ctggcttgtc tacaagtcat gcagcagtaa tggtgggtat tttggggatt ttcaatggat
     6121 ttggtcgctt gctctgggcg agtttgtctg actatatcgg tcgccctcta acctttagta
     6181 tattactgct tgtcaatctt ttcttttctc tctcactttg gctctttaca gattccgttt
     6241 tatttgtagt tgctatgtct attttgatga cttgctatgg agctggtttt tctttgattc
     6301 cagcttatct cagtgatatt tttggaacca aggaattggc cgctctgcat ggttatattt
     6361 taacagcttg ggcaatggct ggtttagcgg gacctatttt attagcagag acttataaaa
     6421 tggctcattc gtacacacaa accttgttcg tttttctcat tttatacagt atcgccttgg
     6481 ctttgtctta ttatctaggt cgttcaatca aaaaagaaag tcaaaaaccg cttacatgat
     6541 tttctgtaga aatggggcta tcttttgctc tggtcttgtg ttatactaga taggttgcaa
     6601 agaaaacagt acttttcttt tgtggaaaag aagcaacacg attttatatc tagttatatg
     6661 aaaatacgtc ataaaaagaa aagtataaac taagccctat agggtggctt cgcaccacct
     6721 ttagaaagaa gaataacgtg aaatttaatg aattaaactt gtctgctgat ttgctagcag
     6781 aaattgaaaa agctggtttt gtagaagcta gtcctatcca agaacaaact attcctttgg
     6841 cccttgaagg caaggatgtt atcggtcaag ctcagactgg tacaggaaaa actgcagcct
     6901 ttggcttgcc tacccttgaa aaaatccgta cagaagaagc gactatccaa gctttggtca
     6961 tcgctccaac tcgtgaacta gctgtccaaa gtcaagaaga actcttccgc tttggtcgta
     7021 gtaagggagt caaagtccgt tcagtatatg gcggatcaag cattgaaaaa caaattaagg
     7081 ctcttaaatc tggtgcccat atcgtggtgg gaactccagg tcgcctcttg gacttgatta
     7141 aacgcaaggc cttgaaatta caagacattg aaacccttat ccttgacgaa gcggatgaaa
     7201 tgcttaacat gggcttcctt gaagacatcg aagccattat ttcccgtgta cctgagaacc
     7261 gtcaaacttt gcttttctca gcaactatgc cagatgccat caaacgtatc ggtgttcagt
     7321 ttatgaaagc ccctgaacat gtcaagattg cggctaagga attgacaaca gaattggttg
     7381 accagtacta tatccgtgtt aaggaacaag aaaaatttga caccatgact cgtctcatgg
     7441 atgtggcaca accagaactc gctattgtat ttggtcgtac caaacgccgt gtggatgaat
     7501 tgactcgtgg tttgaaaatt cgtggcttcc gtgcagaagg aattcatggc gacctagacc
     7561 aaaacaaacg tcttcgtgtc cttcgtgact ttaaaaatgg caatcttgat gttttggttg
     7621 cgacagacgt tgcagcgcgt ggtttggata tttcaggtgt gacccatgtc tacaactacg
     7681 atattccaca agatcctgag agttatgttc accgtatcgg tcgtacaggt cgtgctggta
     7741 agtcaggtca atctattact tttgttgctc caaacgaaat gggttacctt caaatcattg
     7801 aaaacttgac taagaaacgc atgaaaggtc tcaaacctgc aagtgtagaa gaatccttcc
     7861 aatcaaaaaa acaggtagct ctcaagaaaa tcgaacgtga ttttgcagat gaaaccattc
     7921 gtgccaactt tgagaaattt ggtaaggatg ctcgcaaatt ggctgctgag tttactccag
     7981 aagaattggc aatgtatatc ttgagtctga cagtccaaga cccagatagc cttccagaag
     8041 tggagattgc acgtgaaaaa ccactaccgt ttaaaccatc aggtaatggt ttcggtggta
     8101 aagctaaggg aggtcgtgga ggccgtcgtg gggacgaccg tcgagagcgt gatcgccgtg
     8161 gcaatggtcg ccgtgatgag ttcaaaaaag gaagtcgtgg caacgatcgt tttgataagg
     8221 aaaaacgtta ccgtaaggat aataaaaaac cacgcaatac tttaagcgaa aagcaaacag
     8281 gctttgttat tcgtaacaag ggcgataaat aagaaaaagc ggttcaaaat gaatcgcttt
     8341 tttatataag gagaccgaat gtgaaaagac aaataaaatt atta
//
LOCUS       DAVLYR010000083         8218 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLYR010000083 DAVLYR010000000
VERSION     DAVLYR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.083"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            146..793
                     /locus_tag="VYO63_001372"
     CDS             146..793
                     /locus_tag="VYO63_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1672283.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            924..1337
                     /locus_tag="VYO63_001373"
     CDS             924..1337
                     /locus_tag="VYO63_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672284.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1356..1541
                     /locus_tag="VYO63_001374"
     CDS             1356..1541
                     /locus_tag="VYO63_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672285.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(1808..2251)
                     /locus_tag="VYO63_001375"
     CDS             complement(1808..2251)
                     /locus_tag="VYO63_001375"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1672286.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(2281..4503)
                     /locus_tag="VYO63_001376"
     CDS             complement(2281..4503)
                     /locus_tag="VYO63_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1672287.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5519..6148
                     /locus_tag="VYO63_001377"
     CDS             5519..6148
                     /locus_tag="VYO63_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1672288.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(6271..8163)
                     /gene="pepO"
                     /locus_tag="VYO63_001378"
     CDS             complement(6271..8163)
                     /gene="pepO"
                     /locus_tag="VYO63_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1672289.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         2222 a         1893 c         1586 g         2517 t
ORIGIN      
        1 ttattttcag ggttttattt ctatcatttt attttacttt catttggtgg gagaatattt
       61 gaaaaaaaga aaggtagttt aacctatgaa aaaatcacta aaaatttttg ctacatctaa
      121 atagtttgac ctcttcgggg ttgctttggt cgttgggatt gcgattgcat ctggttacct
      181 caactcacac ctcgataaat ttgtagactg gggatcatgg acagctcttg taccctttgg
      241 attgatttcc gtaaccaacg ttgggatttc catgttgtcc actcgtttca cggggaaatt
      301 aagcaaatgg ggaaattact ttggtattgt taataccatt ttgtccggtg ctattgatta
      361 tatccttgga aataaggcgg ccatcattac ctatcccgtc accttcctca tttatacctt
      421 tgctataaag aagtgggaag cttcgcaaga aggcagaccc aaccaaatga gccaaaaaca
      481 ggtaaaattg gcggccatca tcatttccat catcgccttc ctctttgcct ttgtgaccaa
      541 ctatatcgga tatgggggca agatgaatct ccttgcctac gtaacaacta ttgcctttgc
      601 actgtccctc attgccaatg ctttgaatgc attaaaacta acaactcagt ggggcttttg
      661 gttgatttac aatttcgttc agctgacaaa agctggtatt caaggaaact tcgccaatat
      721 cggtaaatac atcttttata tcctcaatgc aatcggagct ttatttgtct ggaatgatga
      781 agaagttaga taatagaagg aaactcaaat aaagaatact agccaactta agagaatctc
      841 ccttctgcta tttttatcat tttagttgta actcccattc ttttggagta gaatgaagtt
      901 agataaaaga gggaggtctt gtcatgaaaa aactctatag aatccatttt atagcaattg
      961 cagttattga tttgctacta tttgcatttt ttatcactag actcgagaca tcttttgagt
     1021 ggctcttgct ctctggttta attttcttcc ttgctcaagg actcctgcta tttctcttgg
     1081 tcgtccgact caaacatcaa ttcgctgaga tttatcctca aatcaataaa aagattcgct
     1141 tctactattt aggggttctc accattgatt ttctattttt tgttctctta gccttcatta
     1201 gttctcagcg tttttcatct cttatgccaa tcatcactgc ttgccattct actttttatt
     1261 atatgacagc tgactaccta agagaaaact atccagactt ttacgacaaa cacatctctt
     1321 tatgggagtg tctctaagga aaaggaggtt ttagcatgaa aaaaatcatc ttcatcaaaa
     1381 ccattcaact ccttgtcatt gatggaatca tgctggcatt tttgacattt aaaagggggc
     1441 ttacttggga ctggattttg atttatagcg gttggctcat tttctttcat cctgtgctat
     1501 tgacctatct ttcaaaccaa ctttgtgacc actttagtta actctattcc cagattagac
     1561 cgagattctg gcgttttgct ttacaaattc tcctatggga tagcctgatg attctctcct
     1621 tggtgtcttt aagtgatatt ccacttttcc ttcagggaac tctcctcatc ctaggacatc
     1681 tcatcccttc ctatcgcatc tgccaaagcc tgaaaagaga cttcccccaa gcatatcaag
     1741 aaccgatttc tttttggagt attttatgat agatgagaaa gaccaagccg actgggcttg
     1801 gtctttctta tctcttttta gtatctagga taatggtaac aggtccatta ttaaccagct
     1861 caacctgcat atctgctcca aagatgcctg tctgaacggg cacttcttgc gctaattttt
     1921 gattgaaagc atcatagaag tctgatgcca tatcaggttt agctgcccct gtaaaggctg
     1981 gacgattgcc tctcttagta tccgcaaaga gggtaaactg agaaatagag aggatttctc
     2041 cttcaatatc tttgacagac aggttcatct tgccttctgc gtctgaaaaa atccgcatat
     2101 tgaccagttt tctcacagca tagtccaaat cttcctcttg gtcctctggt ccaacaccaa
     2161 ccagcaataa aagtccctga ttgatttttc cctgaatctg gccttctata ctcacttggg
     2221 cttttttaac ccgttggata atgattttca taatagcctt tctagtaaga gctaggacaa
     2281 ctagccgttg gtccgtttga cagagtaaac ttctggcaca ctcttaattt tatcgacaac
     2341 cgtggtcagt gtagagaggt tggcaatacc gaaggacaca tggatattag caaacttcat
     2401 atccttggtt ggttgggcat tgaccgttga aatattcttg gttgtatttg aaagaacttg
     2461 cagtacatcg ttcaacagtc ctgtacggtt gagaccgtag atatcgatat gggccatata
     2521 ctccttattt gagctagggt actggtcttc ccattccaca tcaaggagac gttgctcgta
     2581 gttttcttgg gcacgcaggt tcatacagtc cacacggtga atagccacac cacgaccctt
     2641 ggtaatgtag ccaacaatat cgtcaccagg cacggggtta caacacttag caatccgcac
     2701 taggagacca gaagcacctt caataaccac tcccccctca tgcttgacct tgagggtttc
     2761 tttattttca accttgacct cgccaccttt gacaagctcc tctgcctcag ctttggcctt
     2821 ggcacgctct tcctcacggc gttccttttc agtcagacgg ttaaagacgg taatcgcacc
     2881 gatttcccca aaaccaatgg ccgcaaagag ggagtcttct gtcttgtaac tggtcttttg
     2941 cagaacttga tccatgtggc gcttgtccat aaatttattt gccacatagc cattttcttg
     3001 gaactgagcc atcagcatct cacgaccctt gttgacagac aattccttat cttggttttt
     3061 aaagaactgg cgaatcttat tgcgcgcctt gctagtcttg accatattga gccagtcacg
     3121 gctaggtcca aaggagttcg ggttggcgat aatttcaacc tgatcccctg tctttaactt
     3181 ggttgtcagt ggaaccatgc ggccattgac cttggcacca gttgcttttt caccgacctt
     3241 ggtatggatt tcgtaggcaa aatcaatcgg tcctgaatct ttgggaaggg aacggacagc
     3301 tccatctggg gtaaaaacgt aaatctcctc agccaaatag ttttccttaa cagagtccac
     3361 aaattcctta gcatcatcag cctggtcttg gagctccatc atctccttga tccagttcat
     3421 tccaatagct gattccttgc tgttaacttg cccctttata cctttcttat aagcccagtg
     3481 agccgcaacc ccgtactcag ccacctcgtg catttccttg gttcgaatct ggaattcaat
     3541 cggccctttt ggtccataaa cagtcgtatg gatagactga taaccattgg ccttgcggtt
     3601 ggcgatatag tctttgaagc gacctggcat cggtttccaa aattcatgca cgtaaccaag
     3661 catggcataa acatcacttt gggtatctaa aatacaacga atagcaatca gatcatagat
     3721 ttcctcaaac cgttttctct tgtcctgcat tttgcggaaa attgagtaaa tatgcttggg
     3781 acgaccataa atcttccctt tcaagtgacg ttctgtcgta tactcctcta attttgtgac
     3841 tacctcatcc accaaggcct cacgctccct gcgcttttcc ttcatcatat gggtaatctt
     3901 gtaaaactcc gttggattga gataacggaa agacaagtct tctaattccc atttgacact
     3961 ggaaatcccc aaacgatggg caagcggggc atagatttcc atggtttctt tggaaatacg
     4021 ctcctgcttg tcttttcgaa gatgtttcag ggtccgcata ttgtgcaagc ggtcagacag
     4081 tttgaccaaa ataacgcgga tgtcctcaga catggccatg agcatcttgc gatgattttc
     4141 cgctaattgc tcctcgatcg atttgtactc gaccttgcca agcttggtaa ctccgtcaac
     4201 aatcacccgc acatcaggac caaactctct ttccaaatcg tccaaggtcg catctgtatc
     4261 ttccaccaca tcatgcaaga atccacaagc tactgttaca gcatccagct ttagcttagc
     4321 taaaatacct gccacttgga tagggtgaat gatataaggc tcgcctgatt tgcgatattg
     4381 accactgtgg cattcaacag catagaccaa ggccttatgg acaaaatgaa catcctcttc
     4441 cgttaaatat tctttggtta aagcgacaac ttcttcgcct gttaaattca cttctttcgg
     4501 catctctact ctccaattct tcctaccatt ttatcacttt tttaagaata tgaaaactag
     4561 attggaacag aataagaaaa aaataattca aaattgcttg ataattctga attattggcc
     4621 cataatatac tacgaagtta gattttaaac ttaggtgata gaaggagaga tagaagaacg
     4681 gaaaccatat tgtaacccaa agactttctg atttccccaa ttccattgaa gatacgaaag
     4741 ataaacggtg gaactcgtat cacatacact ggtaccttga ctggattttg gaattaatac
     4801 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gttgctcaaa gcactgcttt
     4861 gaggttgtag ataagactga caaagtcagt cacatatata cggcaaggcg acgttgacgc
     4921 ggtttgaaga gattgttgac gcggtttgaa gagatttttg aagagtataa aaatcctcaa
     4981 gatactttct tctatccttt agtttataag gagaatacct atgaaaaaaa ctgctatttc
     5041 tatctttgct ctcctaatgt taggagtttg ctgcctgttc ctattcagcc agcaaagcta
     5101 taaaaaactc gttcaatact atgctaacga ccagaacctg cccagtagga taacttatag
     5161 tgaatatagc gacaaatgat aagccaacta cggtagcact ctaaacatca cgtctatcaa
     5221 acaagctaat gacggagttt atgcaaccta tgaagggcaa ttgacacctt tccaatattg
     5281 ataaattgat aaccagcctg tcttcatcta gtcatgctgg tttttaagtt cattttaaat
     5341 ccttacctat tctccctaac tgtgctatac ttaatttata ctcaatgaaa atcaaagagc
     5401 aaactagaaa gctagccgca ggctgttcaa agcactgctt tgaggttgca gataaagttg
     5461 acgcggtttg aagagatttt cgaagagtat tagtacattc tttgagattg gagctagtat
     5521 gaaaatccat aaaaccgtga atcctgttgc ctatgaaaat acctattatc tagaaggcga
     5581 aaagcacctc atcgtcgtcg atcctggtag tcattgggaa gccattcgtc agacaatcga
     5641 gaagatcaac aaaccgatct gtgctattct cttgacccac gcccattatg accatatcat
     5701 gagtctggac ttggttcgcg agacgtttgg caatcctcct gtctatatcg cagagagcga
     5761 agccagctgg ctctacactc ctgtcgataa tctctccggt ctccctcgcc acgatgatat
     5821 ggcagatgtg gtcacaaaac ctgcagaaca cacctttgtc tttcacgaag aataccaact
     5881 agaggaattt cgttttaagg ttctaccgac cccagggcac tctatcggtg gtgtttccct
     5941 agtctttcct gatgctcacc tagtcttgac gggagatgct ctattccgcg aaactatcgg
     6001 acggaccgac cttccgactg gtagcatgga gcaactcctt catagtatcc agacccaact
     6061 cttcacccta ccaaactacg atgtctatcc aggacatggt ccagctacta ctatcgctca
     6121 cgaaaaggcc ttcaatccct ttttctagca agatgatgac aatcgaaatt taagtaaact
     6181 atccagcaaa tctttctatt acaaaaggca tcctatcaag gttttcacac gtgattggat
     6241 gccttttttc tgatgactag attttttgta ttaccaaata atcacgcgct cctctggtga
     6301 acgccacatt ccgtctcctt ctttgacatc ataggttgta aagaaatcgt cgaagtttgg
     6361 tacttgcaca ttgacacgga gtttggctgg tgcgtgcaca tcgacgctag ccaaaagttt
     6421 cataaattct ggtcgacctt tcatgcgcca gatgcgaccg aagttgtaga agaactcttc
     6481 tgctgagaag tctgcttctc tcttagctgc ttcaagcgct gctgcgattc ctcccaagtc
     6541 agccacgttt tctgatacag tcaatttacc gttaatggtt gctccataag aatcctgtcc
     6601 atcaaattgg tcaatgactt tttgtgtttt ctccttgaag gcagcatagt cgctctctgt
     6661 ccaccaatcc ttgaggctac cattttcgtc aaaggaagcc ccgttagtat caaaggcgtg
     6721 ggaaatttca tgggcaatca ctgccccaat accaccgtag ttagcagaag atgactgatg
     6781 caagtcatag aaaggcgcct gtaaaatggc cgctggaaag acaatcaggt tcttctgagg
     6841 attgtagtag gcattgacca tatgagcagg catgccccat tccttatagt ctacaggctg
     6901 gttccactta ctccaactgt gcttgatttc cacacgcgca aaggctagag cattctcaaa
     6961 aagactggca gtttcattca ctaccttatc cttgtaacgt gcaggcaatt cttctggata
     7021 gccaatataa ggtttgatca cattgagctt cacgatagcc tgtttacagg tttctggagt
     7081 gagccagtca ttcttaagca gacgctcctt atagacatca atcatggttg ccactttttt
     7141 ctccacatcc gccttggctt ctggagagaa cttctcacgg gcgtaccaaa gacccagggc
     7201 ttgcttgaaa ggttcttgtg ctagatgata agctgctttg accttatctt ttgcctctgg
     7261 aactccagaa agggcacggc tgtaggcacc agacaaaaca cggatatcct ctgttaaata
     7321 gctggttgaa agattgacaa cactcaaaat caaggttgct ttaaggagag accaggcttc
     7381 ctcactgtag aattgctctg ctgcttgcca gaaacgttcc tcgtctacaa taaccttgtc
     7441 tggtaattgc ccaataactg ctttgaagaa gtcatccaaa ggtagggcag gcgcgaattt
     7501 cttaaaatct tcgtaagaat atggatgata gagtttagca tattctgaac tttcttcatt
     7561 agagagcacc actgccgcaa ctcggcggtc caattcaagt cttttttcta gcaagtcttc
     7621 aatttcttca tcagagaaat cataagcctt gaggagattt gcgctgcttt ctttccaaag
     7681 agtcaagagc tcttcgcgct gaggatgttc ttctgcatag taggtcgtat ctggcaagat
     7741 tgtgcttgga gcgctagccc atagaacatt gattctagca tccataaagt ctggcgatac
     7801 accaaaagga aggaagtttg gttttcctgc aagctcaaac tctgctagtt tagctgtaaa
     7861 atccgcaaaa gtttccaatt cttggaattc tttaaggagt ggtaagacag gtgtgatacc
     7921 gtcagcttct ctcttgtcaa aatcacgaac taggcggtgg tatttgacaa agttttccaa
     7981 gatagcatcc tcaggcactt cttcacctgc taaccacttg tctgttgtcg ccagcatcag
     8041 gtcttcaatt tcctggtcta aatcaacaaa acctccagtt tgagacttat ctgctgggat
     8101 ttcagctgtc tgttgccatt ctccattgat agcatcataa aaatcatctt gataacgtgt
     8161 catcttgttc tcgctttcat ttgtatttgc atttatctta acaaaaatca ctagggaa
//
LOCUS       DAVLYR010000084         8051 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLYR010000084 DAVLYR010000000
VERSION     DAVLYR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.084"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            104..481
                     /gene="mscL"
                     /locus_tag="VYO63_001379"
     CDS             104..481
                     /gene="mscL"
                     /locus_tag="VYO63_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1672290.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(523..1617)
                     /gene="hemH"
                     /locus_tag="VYO63_001380"
     CDS             complement(523..1617)
                     /gene="hemH"
                     /locus_tag="VYO63_001380"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1672291.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(1695..2570)
                     /gene="srtG2"
                     /locus_tag="VYO63_001381"
     CDS             complement(1695..2570)
                     /gene="srtG2"
                     /locus_tag="VYO63_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
                     /protein_id="HEV1672292.1"
                     /translation="MTVQKRARFKNVFLVFFCVFVALFSWQRVVEASDYDHYNPIEKD
                     ASSTGFETLQHLNKDVCGWISLDGTKVDYPLLQSQDNVKYLDRNAFGDYTIMGSIFLD
                     YRFNPNFTDFNTIIYGHSMASGAMFGEIKKFADKEFFDQHRYGSIYYNGRERGLEIFG
                     ILEVDAYDTEIYRTLSSKDEEHQAYYQYLLSKAKYKRDVSLTSTDKIVLLSTCFLNIT
                     NGRHILLAKITDAPVKVAQDESGEAVGTRYFDHGIPSHWIYILAFLLLIIVLLLLVLI
                     ILIIRRDKKTKEQKK"
     gene            complement(2648..3646)
                     /gene="srtG1"
                     /locus_tag="VYO63_001382"
                     /pseudo
     CDS             complement(2648..3646)
                     /gene="srtG1"
                     /locus_tag="VYO63_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
     gene            complement(3664..4860)
                     /gene="pitB"
                     /locus_tag="VYO63_001383"
     CDS             complement(3664..4860)
                     /gene="pitB"
                     /locus_tag="VYO63_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HEV1672293.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(4905..5456)
                     /gene="sipA"
                     /locus_tag="VYO63_001384"
     CDS             complement(4905..5456)
                     /gene="sipA"
                     /locus_tag="VYO63_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HEV1672294.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(5456..7216)
                     /locus_tag="VYO63_001385"
     CDS             complement(5456..7216)
                     /locus_tag="VYO63_001385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672295.1"
                     /translation="MIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYIGSNDSI
                     TSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVLSKYVQL
                     LPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLEDDAKYVL
                     KFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRTKIKDYS
                     EKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSYRKVTSP
                     VQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQLPSLTV
                     KKILEGEQSPNKSFTINVTLSDKDGKPINGKFGNTTVTNGKAQISLKNSQETALSYLP
                     RDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVIKKVTGAFANLL
                     QSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKILDLPLNA
                     RYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTLVLGVVL
                     PLGGIFFIILLGHLVVNRRK"
     gene            complement(7538..8041)
                     /locus_tag="VYO63_001386"
     CDS             complement(7538..8041)
                     /locus_tag="VYO63_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEV1672296.1"
                     /translation="MNVQYDFKKIQYFTSILVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
BASE COUNT         2266 a         1650 c         1490 g         2645 t
ORIGIN      
        1 ggcctatatt tttagaaaat aatgctccat ttatgagcct tttaattgcc ttctataatg
       61 atttaggata caatgaaaca cgaaacattg aaaggaaaac agtatgttaa aaaatctaaa
      121 atcgttcttg cttcgaggaa atgttattga ccttgctgtc ggtgttgtaa ttgcctctgc
      181 ttttggtgct atcgttactt cacttgtaaa cgacattatc actcctctta ttttaaatcc
      241 cgctttgaaa gctgctaaag ttgaacgtat cgctcaactt tcttggcatg gagtcggcta
      301 tggtaacttc ttaagtgcta ttatcaattt tatctttgtg ggtaccgccc tcttctttat
      361 tatcaagggc attgaaaaag cacagaaact gactggcata aagaaagaaa aaactgccga
      421 aaaaaaacca accgaattgg aagtccttca agaaataaaa gctctccttg agaaaaaata
      481 atcgattaaa aggatctagc gcaaagctaa atcctttttt ctttactctt tgggtaactt
      541 gcctgctttt tcaagcattt ttttgatcag ataaggcatc ttaacattct cccgcccttt
      601 tttctcaatc agttttttca caaattctgg catttgtaaa tcttcagatt cagctaaaat
      661 gtttttcgtc tcgtctgaag gaactaactc atcaaaggtc tcttcttctg ggagaaattc
      721 cttaaactct tgattttcat tgactctaac tgtattgacc aaagcatcaa tcaaacgaga
      781 atctgtattt ggcattggtg gacgatggta gtttacccca aattcctggc ataagtcata
      841 acattctaca tcattgtcaa acaagacctc aatatgctca ctgatgaaac taataggtac
      901 aaaaatataa tggtctggat gttctgtctg ttctctgaga tactccaaaa catctggttt
      961 aatccatgga ataccaatat cactctcact ctgccaagtg ttggtatatt gttctgaact
     1021 caagcccagt ttttcagcga ctagcttgct attttcgaaa atttggtcta tataaggatc
     1081 accaaagtcc aaggcaaaaa tgggcacact atgggctgaa aagatgactt taaagctgtc
     1141 ttgctttact tcttctttta aaatcttagc aatttcatct gcccaatagt ttaagagcac
     1201 ttcttcctga taccagtcct taatgaccaa aaattgaatt tgtttgcttt ctaaaaattt
     1261 ttcatagccc atgacagagt agaaagaata atggggctcc aaaatcaagc agatacattc
     1321 ttcaacacca tcagtttcca tctgcttgat cacgtctgga ataaagggac gagaaaattt
     1381 gttggcaaag tagataccgt actcctcccc taaacgttct ttgactaaat tgacctcctc
     1441 acgtgtaatc cgctgcaaag gcgtcccacc gatacgaata tagttgtcat agagagtctg
     1501 gatttcgtgg tcttggggtc taactccacg acgaatgttt gtaaagaatt ctgctactcc
     1561 ttcaaaacta atctcctctg gagatccaaa ggtcatcatt aatatggctt ttttcatgtt
     1621 atcatcctta tgatattatt ttctctatcc attctatcat gaaagaaaaa aatagtaaac
     1681 gtttcctacg tctactattt cttttgctct tttgtttttt tatctcgtct tataattagg
     1741 atgataagga caagaagtaa gaggacgata ataaggagta gaaaggctag aatgtaaatc
     1801 caatgactgg gaattccgtg atcgaaatac cgtgttccta ctgcttcccc actttcatcc
     1861 tgtgctactt tcaccggtgc atccgttatt ttagctaaaa ggatatgtcg tccattagtt
     1921 atgttaagga aacaggtgct taataaaaca atcttgtccg ttgaggtcaa ggaaacatct
     1981 cgcttgtact tggctttact tagcagatat tgatagtaag cctggtgttc ctcatccttg
     2041 gaactcaagg ttcgataaat ctccgtgtca taggcatcca cttctaaaat cccaaaaatt
     2101 tcaagaccac gttctcgacc attgtagtag atagaaccgt agcgatgctg gtcgaagaat
     2161 tccttatcag caaatttctt aatctcaccg aacatagccc ctgaagccat agagtgtccg
     2221 tagatgatcg tattaaaatc agtaaagttg ggattaaagc gatagtcgag aaaaattgat
     2281 cccattatcg tataatcgcc aaaggcattg cggtcaaggt atttgacatt atcctgactt
     2341 tgtagaagcg gatagtctac cttggtccca tcaaggctaa tccaaccgca aacatctttg
     2401 ttcaagtgct gtagggtttc aaaacctgtg ctcgaagcat ccttttcaat aggattatag
     2461 tgatcatagt cacttgcttc tactactctc tgccaactaa aaagagctac aaaaacacag
     2521 aagaatacca gaaatacgtt tttaaatcgc gctctttttt gaaccgtcat cagcactcct
     2581 ttctatgcga gatttcttaa aaggaaagga ggcgggacaa aaccgcctct tttctattca
     2641 cccttagtca gtaaaagggt ctgcttcttg catttttttt actctctctt ctacgacgta
     2701 ggatcaagta gaggacaagg agtaagaaaa tcaagagcaa gaacaataca aacaagataa
     2761 ggtaccaaat ccatagtggg attgatgaga ggaaacgtcc aagagacgag tcatcaaaga
     2821 cactgtatgg aaatggtttt gatttttttg tatggaaagt atttttaggg acggtgtctg
     2881 taatctttgc gactacgata tgacgaccat tggtcacatc gagaaaacag gtactgagta
     2941 ggatgatacg atcactcggt gagagtgaga tatcccgctt gtgcatggcg actgagagca
     3001 ggtggtctag ataggcctgc tggcggtcct caccctgtat tcctggatca tagatgttaa
     3061 agtcataggc atcaacctcc aacatctcaa agatctcgag ccctttttcc acaccattgt
     3121 agtatatact accgtaacga tgctggtcaa aaaattcctg atcagcaaac ttagccacat
     3181 caccaaacat gaccccattt tctacgtggt gcccgtagat aatggtatta aagtcttcga
     3241 acttaggatt atttcgtgca tcgagaaaga tagcgcctgt cgctgcaaac tcacctttgg
     3301 agtccttgtt gagatacttt tcattgtcct tggcttggac taagggatag tcgatattgg
     3361 tgccatagac attgatccaa ccgaggactt cgggattgag ttcttggagt ttgctgaagc
     3421 ctgaaaaact ggccagctca tcctattgcg tcgtgacagg tcgataagct tcgtactcac
     3481 ttgacgaagc taaggagtag acttggttgg aatcccaaag ggcgtagaaa ccaaagagaa
     3541 agatcaaagc tgcaaaaatg agcagcaagt gattgatcac cccatccaca gcaagaacga
     3601 gacgcctaga cagactcttt tttggtttct cacgcttggt tttcatcatc tgcccccagc
     3661 cgtttaggct tctctacgtt ttttagacaa gacaacaaat ccagccaaac caagaccaat
     3721 catcaaaatg aatggaaggt tatcaatcaa gagaccagta ggagtaacgt cgtcgatttt
     3781 gttagtaact ttgttgtcgt ttttcttttc acctatgagg atatctgtta atgtaaaatc
     3841 tacatttttt tgtccttgag atgtttgttc tgcaccattt gtagtataaa ttgatgaatc
     3901 tgtataacct gctactccag tttcagttac tgtatattta gaacctgctg gtagttgttc
     3961 aactgtaaat gtttcaccat ctttaagagt aaaattgtac tcaactgtat ctgctggggt
     4021 cactactgcc aacacctctg acttagatcc atgttttact ataatattcg taacaggggt
     4081 ttctgctgtt ttatttgtac ttggaagttg aatcttgata tggaattgga aatctcttgt
     4141 tttatcccct gaagctccat caactttttt agtaattgtt aatgctttcg cattggggtc
     4201 tacttgactc ggatcatcag gattaggctc cttacttgcg tctttggtat atgtgttatc
     4261 aaataaatta taaacgcctt gttcacttgg ttctacttta ggggcgttag atttattatt
     4321 ttccttaaag tatactgatg aaatatagac accaccattt actttattct taacaataac
     4381 gtgcatttca tatgtacgtt tgtcgtatct catggcatca attgttttac catttttggt
     4441 aatcgcctgc catccagttt ttgcttccgc tacatcataa acgtattcac cagcatggcc
     4501 ataagcaact cccgaaaaaa tgtcttctgt tgctttttga atcttatcag cagttgggtg
     4561 atcattttta ttgtattcta catttctatc tggaattgca actccggttt gatatggcgc
     4621 accgttagaa gattgtcctg ttttttctgt aaacttaaat gtaaatgtcg ctgttggtgt
     4681 tgttattccc tctgcaatat tcaacgtttt attaatcttc acaacagcat tattttcacc
     4741 atttgctttg gttattgctg aattatcatc cgcaaaagtc gggacacctc tgaatacgga
     4801 caaagcggtt atagctgcaa tgcttgcagt gaatagcttt ttaaagaatg tttttttcat
     4861 gattatttct tttgtttttt tattttcttt tttcattatg atcatcaaaa gcctcgtctt
     4921 ctaatgactg ttacaacttt gccttgagta tcctgtatag ggatggttcc aaaagcacga
     4981 ctgtctacag ccgttgttcg attgtccccg aggacaaaaa gttgtcctgc tgggactttc
     5041 atcgggaagg ttgccccttc cttatagagc agcgtttctt tgtagatatc ttgctcttgt
     5101 tgaggagaac cgttgatgat gagaccattt tcgtttatat cgattgtact tccttcggtt
     5161 gctatgacac gcgccactct ttccttacct ttataactat agactagcaa atcaccaatc
     5221 gaatagcgtt tatccaacct ataatagacg accaagtcgc catcttttaa ggcgggcttc
     5281 atgccatcgt cattgtatct taataatcca aagacgaaaa agtaaaggat ggctagtaca
     5341 aatccaacca tcagaagttt actgactaag taaaggatat ctccccagac actcggcgga
     5401 gacttatccc gattgacttg tgttactggt ttcttatgtt tctttttaag cagcatcact
     5461 tcctcctatt caccacaagg tgaccaagta agatgataaa gaagatccct cctagaggaa
     5521 gaacgactcc aagcacgaga gtgctactca agaagtcaat tcccgtttca ggtacgctgt
     5581 ttttattatt cgtgactgtc gcagacttat ttgcatcaag agttccttct tttttatcat
     5641 aggacacctg atacccacga gacgaacttg cttcttcttc gatactataa cgagcattta
     5701 gaggaaggtc gagaatcttg atggttttat ctttctttag atcaactgtc gccttaccat
     5761 tggtgaattg tagcgttgtt ttttgattat ttactattgc actatacgat ccattcaatg
     5821 gtttattttg cgcatccttt acgttaatgg taatcttaaa ggattgcaga agattagcaa
     5881 aagcacctgt aacttttttt atgactgaaa gtgtcggaag tctgtggttg gtgacgattg
     5941 tttgaacatc ctctgacaaa gtcccctctt gtttttcata ggtgacatga tatcccgttc
     6001 tagagttctc tacttcttcc accttatagt gggtatcacg aggcagataa ctgagggcag
     6061 tttcctgact atttttgaga gaaatctgtg ctttcccgtt agtcactgtt gtattcccaa
     6121 acttgccgtt aatcggcttg ccatccttat ctgaaagggt aacattaatt gtgaaagatt
     6181 tattaggtga ttgttcacct tctaggattt tctttacggt gagacttgga agctgtcggt
     6241 aaataacttt gaaggatggt ttctctttcg tacctgtctt ttgaacttcg actgtgtatc
     6301 ccggagcgtc tggtgctttc agttcgtagt catatccctt agctaccttt gcaaaactag
     6361 tattttcagt aaaactattc gtttgaactg gactagtgac ctttcgatag gaatccttta
     6421 tacttccatc tttcttttgg tttaagttta attcgacact aggtggacga tttgctgatg
     6481 gatttgattt tccatccaca cctttccact caatctctac aggaaccgtc aatggatctg
     6541 acggcttgaa agtgggcttc tcagaatagt cttttatttt ggtacgtgag gtcccaatac
     6601 cataggaata ggcaactttt gcccccttgt tggagtagag tttagtagca tcggcttctt
     6661 cggcatcatc actagtaagt gtcttatcac ccgcaatcgc atcaaatgcc tcttggctag
     6721 aggtgacagt aaacttgaga acgtacttgg cgtcatcctc taaagtgtaa ctcggattaa
     6781 acttggctgt tacttccact aatccctgtt cgttcttctt agtttcaatc gtaacttgat
     6841 tgtcattcag ttcttgctcg ttaccatcct tgattttgac gacacgaagg tgtgaagcat
     6901 caccaggtaa cagctgcacg tacttggaca agacatctga tatagttaca tggtgtacgc
     6961 ctagaggtag aattttatct gtgatttctt tgaatttttg ttgcaagtca ttttcattat
     7021 tggcagaaaa tatttcgttg gggattccag aattataacc actgatatat cctttaagag
     7081 atgtgatact atcgtttgaa cctatatatc tgaatttgat tgaataaaag ccatctattt
     7141 ttggagctat taaactagat atattcagca ttcctctctc taaaccagag gtaaaccaat
     7201 atggagtatc aatcatacgt cccacatttg gattagaata atagttatat attgttctcc
     7261 cactattaga ctcgtaatac atattagctt cgccatctga cagcagaata actacttttt
     7321 ttgccccagc ccttgcactt tgcaacaatc tttgagcttc agttaaccca gcatttatat
     7381 ttgtcccagt gccaatgccc gcgtccatac cataagaacg actagagtca gcaattgtta
     7441 acgaactaac aatatttttt gagctattag aatccgtgct ccaagatact aaagttttag
     7501 catcatcata tgaagagata tttgaatacc aaccagctca atttggccaa gttccatatt
     7561 gataactcag ataatattca ttataataac ggtcattata ctgattatca atctttccgg
     7621 caaaaccaac tatagacaga cggttttggg aattagaaag aattgtatca atgagcccct
     7681 gacgaccatt cacacctttc agtgtatgtt ttagtgcttc aatcctacta atgtatcgtc
     7741 tactagagta agaccacaca tctcgctctt ccatactccc tgaaagatcg gcaaccaaga
     7801 ctacatccaa gggttctgac tgggtctccg ttcccagttt agatgtgata tccagtgaca
     7861 actcatactg gtctttttgc cctgatatcg gagtaatcgt tttgtgcaga gttgtctgag
     7921 gttcagttat tgaatctgca cgtaaagaat taattggtgc acacaaaaaa agaatagcga
     7981 gaaagataac taaaatactg gtaaaatatt gaatcttctt aaaatcatat tgaacgttca
     8041 tgctatctcc c
//
LOCUS       DAVLYR010000085         8033 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLYR010000085 DAVLYR010000000
VERSION     DAVLYR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.085"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            104..2737
                     /gene="polA"
                     /locus_tag="VYO63_001387"
     CDS             104..2737
                     /gene="polA"
                     /locus_tag="VYO63_001387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1672297.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVGTEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2822..3259
                     /locus_tag="VYO63_001388"
     CDS             2822..3259
                     /locus_tag="VYO63_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1672298.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(3495..4505)
                     /locus_tag="VYO63_001389"
     CDS             complement(3495..4505)
                     /locus_tag="VYO63_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1672299.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4654..5823
                     /locus_tag="VYO63_001390"
     CDS             4654..5823
                     /locus_tag="VYO63_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1672300.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5820..6590
                     /gene="recO"
                     /locus_tag="VYO63_001391"
     CDS             5820..6590
                     /gene="recO"
                     /locus_tag="VYO63_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1672301.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6587..7579
                     /gene="plsX"
                     /locus_tag="VYO63_001392"
     CDS             6587..7579
                     /gene="plsX"
                     /locus_tag="VYO63_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1672302.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7585..7818
                     /locus_tag="VYO63_001393"
     CDS             7585..7818
                     /locus_tag="VYO63_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1672303.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            7864..8033
                     /locus_tag="VYO63_001394"
                     /pseudo
     CDS             7864..8033
                     /locus_tag="VYO63_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2282 a         1486 c         2061 g         2204 t
ORIGIN      
        1 ctaaattctc tggctctatc tatttattcg gaaaaagttt gaaaaatact tgctctagct
       61 cttcccaatg gtattttttg attctttcct ttataatggg tgtatggata agaaaaaatt
      121 attattgatt gatgggtctt ctgtagcttt tcgggcgttt tttgcgctgt atcagcagtt
      181 ggaccgtttt aagaatgcgg ctggtttgca taccaatgcg atttatggtt ttcagttgat
      241 gttgagtcat ttgttggagc gggttgagcc gagtcatatt ttggtggctt ttgatgcggg
      301 aaagacgacc ttccggacag agatgtatgc ggactataag ggtggtcggg ccaagactcc
      361 tgatgagttt cgtgagcaat ttcctttcat tcgtgagttg ctggatcata tggggattcg
      421 tcactatgag ctggctcagt atgaggcgga tgacatcatt gggacgctgg ataagctagc
      481 agagcaggat ggttttgata ttaccattgt cagtggggac aaggatttga ttcagctgac
      541 ggatgagcat acggtggttg aaatttccaa gaaaggtgtg gctgagtttg aggcctttac
      601 gccagattac ctcatggaag aaatgggcct cacaccagct cagtttatcg atctcaaggc
      661 gctcatgggt gataagtcgg ataatatccc tggggtgacc aaagtcggtg aaaagacggg
      721 tattaagctc ttgctggagc atggttcgct tgaggggatt tatgaaaata ttgatggaat
      781 gaagacttct aagatgaagg aaaatctcat caatgacaag gaacaggcct ttttgtctaa
      841 aacactagcg accattgata ccaaggcacc gattgcgatt ggtttagagg acttggtcta
      901 tagtggtcca gatgttgaaa atcttgggaa attttacgat gagatgggct tcaaacagct
      961 caagcaggct ttaaatgtgt cgtcagctga tgtggctgag ggtttggatt ttactattgt
     1021 tgaccaaatc agtcaagata tgctgagtga agagtctatc ttccactttg agctttttgg
     1081 tgagaattac catacggata atttggttgg atttgcctgg tcttgtgggg ataagctcta
     1141 tgccacagac aagcttgagc tgttgcaaga cccgattttc aaggatttct tagaaaaaac
     1201 atctctgaga gtttatgact ttaagaaggt taaagttctt ttgcaacgtt ttggtgtgga
     1261 tttgcaggcg cctgcttttg acatccgttt ggctaaatac ctcctttcga ctgtggagga
     1321 caatgaaatt gcgaccatcg ctagtcttta tggtcagact tacttggttg atgatgaaac
     1381 tttctacggt aagggtgtta aaaaggccat tcctgaacgt gagaaattct tggaacactt
     1441 agcttgtaaa cttgctgttt tggtaggaac agagcctatt ttacttgaaa aactcagcga
     1501 aaatgggcaa ttagagcttc tttatgatat ggagcaacct ctggcttttg ttcttgccaa
     1561 gatggaaatt gctgggattg tggtcaagaa agagaccttg cttgagatgc aggctgaaaa
     1621 tgagcttgtc attgaaaaac tgactcaaga gatttacgag ctggctggtg aggagtttaa
     1681 tgtcaactcg cctaagcagt tgggcgtgct tctctttgag aaattgggac ttcctctaga
     1741 atacactaag aaaaccaaga caggttattc gacagcagtg gatgtgttgg agcgtctcgc
     1801 tcctattgct ccgattgtta agaaaatcct ggattaccgt caaattgcta agattcaatc
     1861 tacttatgta attggcttgc aggactggat tttggctgat ggaaagattc atactcgcta
     1921 tatgcaggat ttgacccaga ccgggcgttt gtctagtgtg gatccaaact tgcaaaatat
     1981 tcctgctcga ttggaacaag ggcgcttgat tcggaaggct tttgtgccag agtgggagga
     2041 tagtgtgcta ctcagctctg actattcaca gattgaattg cgcgttttgg cgcatatttc
     2101 taaggatgag cacttgatta aggccttcca agagggggca gatatccata cttcgacagc
     2161 catgcgggtc tttggcattg agcgtcctga taatgtgact gcaaatgacc gtcgcaatgc
     2221 caaggcagtt aactttggag tggtttatgg gatttcagac tttggcttgt ctaataattt
     2281 gggaattagt cgtaaggaag ccaaagccta cattgatacc tactttgaac gttttccagg
     2341 tattaaaaac tacatggatg aagtggtgcg ggaggcgcgt gataagggct atgtagagac
     2401 cctctttaag cgtcgccgtg agttgccaga tatcaattcg cgcaacttca atattcgtgg
     2461 ttttgcggag cgaactgcta tcaactcacc tatccagggt tcggcagcag atattctcaa
     2521 gattgccatg attcagctgg ataaagcctt ggttgcaggt ggttatcaga ctaagatgct
     2581 gttacaagtg cacgatgaaa tcgtccttga agtgcctaaa tctgaattgg tagagatgaa
     2641 aaaattggtg aaacaaacca tggaagaagc cattcaactc agtgttcctc ttatcgcaga
     2701 tgagaatgaa ggggcaacct ggtacgaggc taaataaaaa gggggctagt cctccttttt
     2761 tgtagtagaa ttctgcaaac cttttcaaaa tgtgctatac tgatgaaaaa ggaggatttc
     2821 tatgagtcaa gaatttatca atccaagtga tggcgtgatt cgtcagtatc tcgcaacgag
     2881 taaaaccctt gctgtggtgg gtttgtctga ccgtgaagaa acaactagca atcgagtaac
     2941 aaaggaaatg caggctcggg gctataaaat catcccagtc aatcccaagg cggcaggtgg
     3001 cgaaatcttg ggtgaaaagg cttatgctag cctcgctgaa attccttttc ctgtagatat
     3061 tgtcaatgtt tatcgtcgca gtgaatttct gcccgatgtg gcgcgtgatt ttctcaaagc
     3121 tgatgccaag attttttggg cacagctagg acttgaaagt ttagaagcga aagagatttt
     3181 gcgtgatggt ggatgtgatg atatcgtgat gaatcgctgt atcaagagag aacatactcg
     3241 cttgattgag gaagcataag aaaaaggtag ctgatgggct accttttgtg ttatactcaa
     3301 tgaaaatcaa agagcaaact agcaaactag gaaactagcc gcgggctgct caaaacactg
     3361 ttttgaggtt gtagatagaa ctgacgaagt cagctcaaaa caccgttttg aggttgcaga
     3421 tagaactgac gaagtcagta acatatatac ggcaaggcga cgttgacgtg gtttgaagag
     3481 attttcgaag agtattagaa aatgccgata agggtctgca taccaagagt ggtgaggatg
     3541 atggcaatcc agcagatggc tccgagaaca atggattttc cactggattt gaccatagcg
     3601 accagattgg ttttgagacc gatagcactc atggccatga caatgaggaa tttagagagt
     3661 tgtttgagag gggtaaagaa actactagac acaccgagag aggtcagaag ggtggttagg
     3721 agcgatgcaa ggataaagta aaggataaaa agtgggaaga cttttttcag ttgtaggctt
     3781 tgcttgtttt cttgttggcg actttgccag taggatagaa agagcgtgat agggataatg
     3841 gccaaagtac gtgtgagttt aacaatggtt gcagactcga gggtattgct ttggtaaaga
     3901 ctgtcccaag cgctggcggc agccgttaca gaggaagtgt cgttgaccgc agtccctgca
     3961 aagagggcga agccttcatt ggatagatga agccaggtgc cgagggttgg aaagatgagc
     4021 gcagccaaga cattgaagaa aaagataacg gaaatggctt gggctacttc cttttcctta
     4081 gcatcaataa cgggcgctgt cgctgcaatg gcagaacccc cacagataga agaacctact
     4141 ccaaccaagg tagccagttt tgtatccagt gcaaagaaac gctggaagag gtaggcaata
     4201 atcagagcta ttgagatagt ggacaggatg acagggagtg aagattggcc aactgcgaag
     4261 acctgcgaga tattgagacc aaaaccaagc aagacaacgg catattggag caacttcttg
     4321 gaactaaagg tcaaaccagc atccagttgt ttataggacg agagaaaggg atgtaggagc
     4381 atgcctatga aaatggcaaa aacgggcgcg ccaatcacag ggaagaatcc tcctaagtac
     4441 caagatagga tggaaatgag aaggcaggcc aagatacctg ctccattttt tgatagaaat
     4501 gacataaaaa cctccaaata aaatctatta ccattataga cctataaaca ggaaaagtaa
     4561 aatagaaagt ggaaagctat tctctaatgt atttttgcgg tcggggggct tttgtagtat
     4621 aatagagata cgttttgaaa gtaggaggta tctatggact taactaagcg ctttaataaa
     4681 cagttagata aaattcaagt ttcgttgatt cgtcagtttg accaggctat ttcggagatt
     4741 cctggggtct tgcgtttgac cttgggggaa cctgatttta caacgccaga ccatgtcaag
     4801 gaggcggcca agcgagcgat tgatcagaac caatcctact atacagggat gagtggtctg
     4861 ctgactctac gtcaggcagc cagtgacttt gttaaggaaa agtaccaact ggactatgct
     4921 cctgaaaatg aaatcttggt tacaattggg gcgacagagg ctttatctgc gactttgacg
     4981 gctattttgg aagagggaga caaggtactt ttgccagctc ctgcttatcc aggctatgaa
     5041 ccgattgtta acttagttgg ggcagaaatt gttgagattg atacgactga aaatggtttt
     5101 gtcttgactc ctgagatgtt ggagaaggcc attttggagc agggtgataa gctcaaagcg
     5161 gttattctca actatccagc caatccgaca ggaattacct acagtcgaga gcagttagag
     5221 gccttggcag ctgttttacg caagtacgaa gtttttgttg tctgtgatga ggtttactca
     5281 gaattgacct acacaggcga agcccatgtg tctctaggaa cgatgttgag agaccaggct
     5341 attattatca atggtttgtc taaatcgcat gccatgacag gttggcgttt ggggctgatt
     5401 ttcgctccgg cgaccttcac agcccagtta atcaagagtc accagtactt ggtcactgcc
     5461 gcaaatacca tggcgcaaca tgctgcggta gaagccttga cggctggtaa aaatgatgcg
     5521 gagcccatga agaaggaata tatccagcgt cgggactata tcatcgaaaa aatgactgct
     5581 cttggttttg agattatcaa accagacggt gccttctata tttttgctaa aattccagcg
     5641 ggctacaatc aagactcctt tgcttttctg aaggattttg ctcagaagaa ggccgttgcc
     5701 tttatccctg gtgcagcctt tggacgttac ggggaaggct acgtccgcct atcttatgca
     5761 gccagcatgg agactatcaa agaagccatg aaacgacttg aggagtacat gagagaagca
     5821 tgattcagtc tatcacgagt caaggcttgg tgctttacaa tcgcaatttt cgtgaggatg
     5881 acaagctcgt caaaattttt acagagcagg ttggcaaatg catgtttttt gtcaaacacg
     5941 ctggtcagtc taagctggcg cctgttattc agcccttggt gctggcacga tttctcttgc
     6001 gaatcaatga tgacggactc agttacatcg aagactatca tgaggtcatg acttttccca
     6061 agattaatag tgacctcttt gtcatggcct atgcgaccta tgtggcagct cttgcagatg
     6121 ctagtttgca ggacaatcag caggatgctc ccttgtttgc ttttttgcaa aagactttgg
     6181 agttgatgga agcaggcttg gattatcagg ttttgaccaa tatttttgaa attcaaattt
     6241 tgactcgatt tggaatcagc ctcaatttta atgagtgtgt cttctgccat cgggttggtc
     6301 aggcttttga cttttctttc aaatatggag cctgcctctg tccagagcat tatcatgagg
     6361 ataagagacg ttgtcatctc aatcccaata tcccctatct gctcaatcaa tttcaagcta
     6421 ttgattttga gactttggag accatttcgc tcaagcctgg aatcaagcaa gagctacgcc
     6481 aatttatgga tcaattatat gaagagtacg ttgggattca cctaaaatca aagaaattta
     6541 ttgattccct agcagactgg ggacaattac taaaagagga aaagaaatga aaaaaatcgc
     6601 agtagatgcc atggggggcg attacgcacc tcaggccatt gttgagggtg tcaatcaagc
     6661 cctatctgac ttttcagata tcgaggttca actttacgga gatgaagcta aaatcaagca
     6721 atatctgaca gcgacagagc gcgtcagcat tatccatacg gatgagaaga ttgattcgga
     6781 tgatgaacct acgagagcta ttaggaataa gaaaaatgcc agtatggtat tggcagccaa
     6841 ggctgtcaaa gatggtgaag cagacgctgt cctttcggct gggaatacag gtgccttgtt
     6901 ggcagcagga ttcttcatcg tgggtcgtat caagaatatc gaccgtcctg gactcatgtc
     6961 taccttgcct accgttgatg gaaaaggttt tgacatgcta gaccttggtg ccaatgcaga
     7021 aaatacagcc cagcacctcc atcaatatgc ggttctaggt tccttctatg ctaaaaatgt
     7081 ccgtggcatt gcgcaaccac gcgttggttt gctcaacaac ggaacagaga gtagcaaggg
     7141 cgacccgctt cgtaaggaaa cttatgaatt actggcggct gatgaaagtt tgaactttat
     7201 cggaaacgtg gaagcgcgtg atttgatgaa tggcgttgca gatgttgttg tggcagatgg
     7261 tttcacggga aacgctgtgc tcaaatccat cgaagggaca gctatgggaa tcatgggctt
     7321 gctcaagaca gctattacag gtggtggtct tcgagcgaaa ctaggtgccc tccttctcaa
     7381 ggacagcctc agaggtttga aaaaacagct caattattca gatgttggtg gagcggtctt
     7441 gtttggtgtt aaggcacctg ttgtcaagac tcatggctca agcgatgcca aggctgttta
     7501 tagtacgatt cgccagattc gtaccatgct agaaacagac gtagttgccc agactgcgcg
     7561 tgaattttca ggagaataaa agagatgaca gaaaaagaaa tttttgaccg tattgtgacc
     7621 attatccaag agcgacaggg agaggacttt gtcgtgacag aatccttgag tctgaaagac
     7681 gatttggatg cggattctgt tgacttgatg gagtttatct tgaccctgga agatgaattt
     7741 agtatcgaaa tcagcgatga agaaattgac caactccaaa acgtaggaga tgtggttaaa
     7801 atcattcaag gaaaatagca atcggagttc caagtcaacg gaagtagatg gtttttagaa
     7861 atgagaaata tcggacaagc tggtaaaatc ttggctgaca gtggttatca agggctcatg
     7921 aagatatatc ctcaagcaca aactccacgt aaatccagca aactcaagcc gctaacagtt
     7981 gaagataaag cctgtaatca tgcgctatct aaggagagaa gcaaggttga gaa
//
LOCUS       DAVLYR010000086         7931 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLYR010000086 DAVLYR010000000
VERSION     DAVLYR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.086"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            113..694
                     /locus_tag="VYO63_001395"
     CDS             113..694
                     /locus_tag="VYO63_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1672304.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETSGDLTIVIQKKQ
                     GAPSAKSKMEGVFGNCEILKKDKGYYILRSVKE"
     gene            691..1968
                     /locus_tag="VYO63_001396"
     CDS             691..1968
                     /locus_tag="VYO63_001396"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1672305.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILVPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            1986..2648
                     /gene="deoC"
                     /locus_tag="VYO63_001397"
     CDS             1986..2648
                     /gene="deoC"
                     /locus_tag="VYO63_001397"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1672306.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            2635..3024
                     /locus_tag="VYO63_001398"
     CDS             2635..3024
                     /locus_tag="VYO63_001398"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1672307.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3114..4166
                     /locus_tag="VYO63_001399"
     CDS             3114..4166
                     /locus_tag="VYO63_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1672308.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4310..5845
                     /locus_tag="VYO63_001400"
     CDS             4310..5845
                     /locus_tag="VYO63_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672309.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            5838..6896
                     /locus_tag="VYO63_001401"
     CDS             5838..6896
                     /locus_tag="VYO63_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672310.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            6899..7855
                     /locus_tag="VYO63_001402"
     CDS             6899..7855
                     /locus_tag="VYO63_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672311.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2235 a         1441 c         1905 g         2350 t
ORIGIN      
        1 ttgaagaaaa tggcttcgtg actcgctttc ttcggtcttt ttcgttttat tatcaatttg
       61 taaacgattt acaatttgag tcaaattatg gtaaaatagt gtcatgagta aaatgtatta
      121 tgcagaaaat cctgacgctg ctcacgacat tcatgagttg agagtagagt tgttgggaca
      181 aaaaatgacc tttttaacgg atgcgggtgt ttttagcaag aaaatagttg actttggaag
      241 tcaactattg ctcaagtgtc ttgaggtcaa ccaaggagaa actgtccttg atgtaggctg
      301 tggttacgga cctttgggtt tgtccttagc taaggcttat ggagttcagg ctactatggt
      361 tgatatcaac actcgtgcct tagatttagc tcggagaaat gctgaaaaaa ataatgcaaa
      421 agcgacgatt ttccagtcca acatctatga acaagttgaa ggacattttg atcatgtcat
      481 ttccaatcct cctatccgtg cgggcaagca agtcgttcat gaaatcattg agaaaagtaa
      541 agatttcttg gaaactagtg gggatttgac aatcgtcatc cagaaaaaac aaggggctcc
      601 aagtgctaag tccaagatgg agggtgtgtt tggtaattgt gaaatcctta aaaaagacaa
      661 gggatactat atccttagaa gtgtgaaaga atgagagcag ttgatttaat acaaaaaaaa
      721 cgagacggtc aagaactgac ttcaagtgaa attgaatggc tggtagaagg ctatgtgtca
      781 ggaactgttc ctgattatca gatgtctgcc tttgctatgg ctgtttattt taaaggaatg
      841 acgactcgag aaatctctga tttaacgatg aatatggtta agactgggca agagtttgac
      901 ctgtcagcca ttgatggtgt gaaagttgac aagcattcta ctggtggtgt cggcgataag
      961 gtaaccttga ttttagttcc tcttgttgct agcttcggtg tgcctgtagc aaaaatgagt
     1021 ggtcgtggtc ttggccatac aggcggaaca attgataaat tggagtccat taagggctat
     1081 caagtagaac gtagtcaaga ggattttatt cgtcaggttc aggatattgg agtatctgtc
     1141 attgggcaat cagaccagct ggtcaaagca gataagcttc tctatgccct tcgtgatgtg
     1201 accgcaactg tcgacacgat tcctttgatt gcgagttcgg tgatgagcaa gaaaattgcg
     1261 gcaggggcgg atgctatttt gctagacgtg actgtcggtg agggtgcctt catgaagacg
     1321 gttgatgagg cgcgtgagtt ggctcaaact atggtagatc ttggtaaggt tgttggacgg
     1381 aagacagtag cagtgattac ggatatgagc cagcctttgg gacgagcgat tggaaatcgt
     1441 ctggaaatcc ttgaagcatt ggagattttg caaggtcaag gacgtcagga tattacccac
     1501 tttatttgtg aattggctca aattatgctt ggtttggcaa atgttaacaa aactgttgaa
     1561 gaagttcgcc aacatcttga gaatggtcaa gcactggcta agtttgagga aatggtgcaa
     1621 gcccaaggtg gagacttgga agacctctat cgtcctgtaa atgtagctca tgtggtggaa
     1681 atccctgctc aggaaacggg tgtcatttca gctcttccag ctatggactt tggtctttat
     1741 gccatgaggc tgggtgctgg tcgtgcagtt aagtctgatg ccttggacta tgaaacagga
     1801 attgtttttg aaaagaagat tggagactcc gttcaaaagg gtgaaattgt tgcaaaagta
     1861 tacacaaatg aaaaaattcc acctcaacta gttacagatt tccaaaaatg tgttaaaata
     1921 agtgatgaag tgaaaaaaat ccgtgaaatt gtagaaatca tctcctaaat ggagggaaaa
     1981 ccgaaatgaa attaaataaa tatatagatc atacgctttt aaaacaagat gcaaagaaaa
     2041 aacaaattga tagtttgttg tctgaggcta gagagtatga ctttgccagt gtttgcgtta
     2101 atccgacctg ggttgaacat gctaaaaaag gacttgaagg cacagatgtt aaggtttgca
     2161 cagtagtagg tttccctttg ggagcaacaa cttcagccgt gaaagcattt gagacaaaag
     2221 aagctatcca aaatggtgca gatgagattg atatggtgat caatgttgga gctctcaaat
     2281 caggtaattt agccttggtt gagtcagata ttcgcgcagt agtggaagca agtggtgata
     2341 agttagtgaa agtcattatt gaagcttgcc ttctgacaga ccaagaaaaa attgttgttt
     2401 gccaattggc ccaaaaagct ggggctgact ttgttaaaac atctactggt ttttcaactg
     2461 gtggtgctac gatagcagat gttacattaa tgcgtgaaac agttggatct gatatgggag
     2521 tcaaggctgc tggtggagct cgttcttatg cagatgctct tgcctttgtc gaagcaggtg
     2581 cgacccgtat cggaacgtca gctggggtag ctattttaaa aggagaattg gcagatggcg
     2641 actactgagt tgattgaact ggcaattgaa accagcaaac atgcatatgt cccctattct
     2701 cactttccta tcggagcagt tttagtagcc aaagacggaa gtgtttacac gggagtgaat
     2761 atcgaaaatg ctagctatcc tttgaccaat tgcggtgaga gaacagctat ttttaaggct
     2821 atatctgaag gccaaagaga attttcagaa ttgattgtct atggtcaaac tgaaaaaccg
     2881 atctcgccat gtggtgcttg tcgccaagtg atggctgaat tttttgaaca agatctaaaa
     2941 gtgaccttag tcgcaaaaga taaatcgacg gtcgagatga cggtcgggga attacttcca
     3001 tactctttta cagacttaaa ctagtctgag tcgctctctg agtggcacgg tccttgtggc
     3061 caaccaatcc atacttgcaa catcgttgca catcttattt aggaggttca gtaatgaaca
     3121 agaaacaatg gctaggtctt ggcctagttg cagtggcagc agttggactt gctgcatgtg
     3181 gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt gaagacaaaa gcagcaatcg
     3241 tcactgatac tggtggtgtt gatgacaaat cattcaacca atcagcttgg gaaggtttgc
     3301 aggcttgggg taaagaacac aatctttcaa aagataacgg tttcacttac ttccaatcaa
     3361 caagtgaagc tgactacgct aacaacttgc aacaagcggc tggaagttac aacctaatct
     3421 tcggtgttgg ttttgccctt cataatgcag ttaaagatgc agcaaaagaa cacactgact
     3481 tgaactatgt cttgattgat gatgtgatta aagaccaaaa gaatgttgcg agcgtaactt
     3541 tcgctgataa tgagtcaggt taccttgcag gtgtggctgc agcaaaaaca actaagacaa
     3601 aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat ctctcgtttt gaagcaggat
     3661 tcaaggctgg tgttgcgtca gtagacccat ctatcaaagt ccaagttgac tacgctggtt
     3721 catttggtga tgcggctaaa ggtaaaacaa ttgcagccgc acaatacgca gccggtgcag
     3781 atattgttta ccaagtagct ggtggtacag gtgcaggtgt ctttgcagag gcaaaatctc
     3841 tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat cggtgttgat cgtgaccaag
     3901 aagcagaagg taaatacact tctaaagatg gcaaagaatc aaactttgtt cttgtatcta
     3961 ctttgaaaca agttggtaca actgtaaaag atatttctaa caaggcagaa aaaggagaat
     4021 tccctggcgg tcaagtgatc gtttactcat tgaaggataa aggggttgac ttggcagtaa
     4081 caaacctttc agaagaaggt aaaaaagctg tcgaagatgc aaaagctaaa atccttgatg
     4141 gaagcgtaaa agttcctgaa aaataatgga tggaagtcat ttcttaggaa gcggccaagg
     4201 gccgcttttt taagagttga gacaaagtca ttttgtctca gtaaagcttg ctactagaca
     4261 aactagcaag ttttattgaa ataaaataag actctgaaag gaagagcaca tggcacacga
     4321 aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt ggatttgttg ccaacgacaa
     4381 aatcaacttg cacctacgaa aaggtgaaat tcatgcactt ttaggagaaa atggggctgg
     4441 taagtccacg ctaatgaaca tgttagcagg ccttcttgaa ccaactagtg gtgaaatcgc
     4501 ggtcaacggt caagttgtca atctcgactc cccatctaaa gcagctagct tgggaatcgg
     4561 gatggttcac cagcacttta tgttggttga agccttcaca gtggctgaaa acatcatttt
     4621 aggtagtgaa ttgactaaaa atggtgtgct agatatcgct ggagctagca aagaaatcaa
     4681 ggctctttct gaacgttatg gcttagctgt tgacccttct gccaaggtag cagatatctc
     4741 agttggagcc caacaacgtg tagaaatttt aaaaacactt tatcgggggg ctgatatcct
     4801 tatctttgac gaaccaacgg ctgttttgac tccatcagaa attgatgagt tgatggctat
     4861 tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg attacccaca aattggatga
     4921 aattcgcgca gtttctgacc gtgttacagt tatccgtcgt gggaaatcaa ttgaaaccgt
     4981 tgaaattgca ggggctacca atgctgattt ggcggaaatg atggtaggac gttctgtttc
     5041 ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg gttttgtcta tcaaagattt
     5101 ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat ctgtccttgg atgttcgtgc
     5161 tggagagatt gttggtattg cggggattga tggaaatggt cagtctgaac tgattcaagc
     5221 cattacaggt cttcgtaagg ttgaatctgg tagcattgag ctaaaagggg attcaattgt
     5281 aggcttgcac ccacgtcaga ttacagaact aagtgttggg cacgttccag aagaccgtca
     5341 ccgtgatggc ttgattttgg aaatgatgat atctgaaaat attgcccttc aaacctacta
     5401 taaagaacca catagtaaaa atggaatttt gaattattca aatattactt cttatgctaa
     5461 aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa ttagttcctg cagctgcact
     5521 ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa attgatcgag atcctgatct
     5581 ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt gccattgagt atatccacaa
     5641 acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt gttgtcagct ttgaattgga
     5701 tgagatttta aacgtctcag accgtattgc cgttatccac gatggtaaga ttcaaggtat
     5761 tgtatcacca gaaacaacca ataaacaaga acttggtgtc ttgatggctg gtggaaactt
     5821 gggaaaggag aagagtgatg tttaaaaaat tacaacaaat ttcggttccc ttgatttctg
     5881 tatttctagg aattttactc ggagccattg tcatgtggat cttcggttat gatgctattt
     5941 ggggctacga agaattgttc tatacagcct ttggcagtct gcgtgggatt ggagaaatct
     6001 tccgtgctat gggtcctctg gtcttgattg gtcttggttt tgcggttgcc agtcgagctg
     6061 gtttctttaa cgtcggactt cctggtcagg ctttggcagg ttggattctc agtggttggt
     6121 ttgccctgtc gcatccagat atgccccgtc ccttgatgat tctagcaacc atcgtgattg
     6181 ccttgattgc tggtgggatt gtcggagcga ttccaggtat tcttagggcc tatctaggga
     6241 cgtcagaggt tattgtaacc atcatgatga actacattgt cttgtatgta gggaatgcct
     6301 ttatccatgc tttccctaaa gacttcatgc aaagtacaga ttcgaccatt cgtgttgggg
     6361 ctaatgcaac ctatcagaca ccttggttgg ctgagttgac tggtaactca cggatgaata
     6421 ttggtatttt ctttgccatc attgccgttg cagttatttg gttcatgctc aagaaaacaa
     6481 ctcttggttt tgaaatccgt gcagttggtc ttaatccaca tgcttcagaa tatgctggta
     6541 tttctgccaa gcggactatt atcctatcta tgattatttc aggtgccttg gcaggtcttg
     6601 gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta tgttcaaggt tcgtcattag
     6661 ctatcggatt taacggaatg gcggttagtt tgcttgcggc caactcacca attggtatac
     6721 tctttgcagc cttcctattt ggcgttctcc aagttggggc tcctggtatg aatgctgcgc
     6781 aggtaccatc tgagcttgtc agcattgtaa cagcgtctat tatcttcttt gtcagtgttc
     6841 attaccttat cgaacgcttt gttaaaccga aaaaacaagt taaaggaggt aagtaaagat
     6901 gtctattata accttgctcc ccctcttggt gtcttctatg ctgatttact cagcacctct
     6961 catctttaca agtatcggtg gtgttttctc tgaacgtggt ggtgtggtaa acgttggcct
     7021 tgaaggaatt atggttatgg gtgccttttc tggagttgtc tttaaccttg aatttgcaga
     7081 acaatttgga gcagcaactc catggctatc cttgcttgta gcaggattgg ttggtagtgt
     7141 tttctctatc atccacgcag cagcgacggt tcatttccgt gcagaccatg ttgtcagcgg
     7201 tacggtattg aacttgatgg cgcctgcctt ggctgttttc ttagttaaag ttctttataa
     7261 caaaggacaa accgacaacc taagtcaaac ttttggacgc tttgatttcc cagtcttggc
     7321 aaatatccca gtgattggtg atatcttctt caagtcaact agtctacttg gttatctggc
     7381 gattgccttc tcattccttg cttggtttat tctctttaag actcaatttg gtcttcgtct
     7441 ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg ggaatcaacg tctacaagat
     7501 gagatattta gggattatta tttcaggttt tctaggtgga attggcggag cgatttatgc
     7561 gcaatcaatc tcagttaact tctcagtgac aactattgtt ggacctggat ttatcgccct
     7621 tgctgcgatg atctttggga aatggaatcc aatcggtgct atgctttcta gtctcttctt
     7681 tggactttca caaagtttgg ctgttatcgg ttctcaattg ccgttcctac aaggagtgcc
     7741 agcggtttat cttcaaattg caccttatgt tttgacaatt cttgtcttag cagccttctt
     7801 tggaaaagca gtcgcaccaa aagcagatgg tatcaactac atcaaatcaa aataagcata
     7861 caaaaaaacg tcagttcaat gactgaactg ccccccaaaa gttagacaga aaaaatctaa
     7921 cttttggggt g
//
LOCUS       DAVLYR010000087         7797 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLYR010000087 DAVLYR010000000
VERSION     DAVLYR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.087"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(117..1253)
                     /gene="lctO"
                     /locus_tag="VYO63_001403"
     CDS             complement(117..1253)
                     /gene="lctO"
                     /locus_tag="VYO63_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1672312.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1878..3368)
                     /gene="lysS"
                     /locus_tag="VYO63_001404"
     CDS             complement(1878..3368)
                     /gene="lysS"
                     /locus_tag="VYO63_001404"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1672313.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINKPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3496..3642)
                     /locus_tag="VYO63_001405"
     CDS             complement(3496..3642)
                     /locus_tag="VYO63_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1672314.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            3675..4592
                     /locus_tag="VYO63_001406"
     CDS             3675..4592
                     /locus_tag="VYO63_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275026.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1672315.1"
                     /translation="MYIAVWGSLGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSAMQDSGIQVLFQGNNLLRILQGL
                     GVTIGISILSVLLSMMFGTVMGIIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFG
                     LARNFNINISGETSVIIVFTLWGTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYY
                     HIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTTSFW
                     IYGTILILYFAVCYPISKLSTHLEKHWRN"
     gene            4593..5351
                     /locus_tag="VYO63_001407"
                     /pseudo
     CDS             4593..5351
                     /locus_tag="VYO63_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            5363..6223
                     /locus_tag="VYO63_001408"
     CDS             5363..6223
                     /locus_tag="VYO63_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1672316.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGH"
     gene            complement(6303..6950)
                     /locus_tag="VYO63_001409"
     CDS             complement(6303..6950)
                     /locus_tag="VYO63_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672317.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKPGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(6947..7645)
                     /locus_tag="VYO63_001410"
     CDS             complement(6947..7645)
                     /locus_tag="VYO63_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672318.1"
                     /translation="MGKLSSYMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
BASE COUNT         2365 a         1678 c         1410 g         2344 t
ORIGIN      
        1 actgtcctga ttaatttgtc ctattcttat ttcattttat tatagttttc gatagcgatt
       61 tattcttcca atacacgaag aaaaacctcc acattcagtg gaggcaatct gttttatcaa
      121 tacaatttta agtcacgagg gtcaactggg aaggttgggt tgtatggatt gtgacggagc
      181 ttaaagtgtt tgacatcttc aatggtctga gctccagaca attgcataac tgtcttcaat
      241 tccgcattca agtgttcaaa gacttgacgc acaccgacac taccaccgag agccaagcca
      301 taaataacag ggcgaccaat agctaccaag tctgctcctg aagccaaggc tttaaagacg
      361 tgttgaccac gacgaacacc agagtcaaag acaatcggca cacgtctatc aactgcttct
      421 gccacttctt gaagcgagtc aaaggcagct ggtccaccgt cgatttgacg accaccgtgg
      481 ttggttaccc agataccaga agctcctgca gcaagtgaac gttcaacgtc ctcacggcat
      541 tgtggtccct tgacatacac aggaagacca gagtattcag cgataaattc tacatcgcgt
      601 ggagacaagc gttgtttagc tgatttgtaa acaaagtcca ttgatttacc agcaccttct
      661 ggcaggtatt cttcaacaat cggcatgcca actgggaaga caaaaccatt acgcttatcc
      721 acttcacgat tcccccctac agtagcatct gccgtcaaga caatcgcttt ataaccttca
      781 gccttcacac ggtccatgat gtggcggttg ataccgtcat ccttactaaa gtaaaattga
      841 aaccaatgag gtgtcccttg aagggcttca gaaatctctg gaaggtcaac agtagagtaa
      901 gaactggttg tataaagaga accaaactca tgcacaccac gcgcagtcgc cacttccccc
      961 tgttcattcg ctaatttatg ggccgcaaca ggcgccataa tgattggtga agatagtttt
     1021 tcacctgcaa attcaatctc tgtacttgga ttttcaacat cgcaaagtgt atgaggaacg
     1081 atgagtttgt ggttaaaggc gcggatattc tctcttaaag tgaaagtatc ttccgcccca
     1141 ctagcgatat agccaaatgc tgctttagga ataacttgtt gcgccattgg ctccaaatca
     1201 taggtattga tgaagtctac atgaccttct gcattgcttg ttttgtatga cataaaatgc
     1261 cctccttgat taagtaagcg tttgcttttt actacacaac tataataact cttttttcaa
     1321 tcattttcaa gagttttgct taaaaaccat cttatttttt catacaatat actagcccta
     1381 ctttccttaa actctaaact ttagtctata ttaagtgaat cgttgagatt ttttgctgcc
     1441 aggcatttga actacttttt tatttgcgga ttctatctac taatactata aaaaaacaag
     1501 tgaataagac accagaacat cctctaatag gacctatgga cttatatcag tcccatatat
     1561 actgtttaag caagatacta tcctagttga cgctaacttc ctatcccact tattaatttt
     1621 ttatttatag tgaatctgaa caaatcaatt gggaaatcca agtcaatttc tagtactttt
     1681 tgaacagttt cagcgtacta tcgtaaaatg aaataaaaca tgcacaaatt gattagggaa
     1741 tttaaaccaa tttctaacag tgtcttagaa atcaaagtgt actatttcaa cttcaatata
     1801 ctacacttca ttttctcctt aaattcagtc tctttttgaa tcaaaaaatc ccctggtaag
     1861 agccagaggg taagaattta tttcattgtt gggaagagca atacatcacg gatagttgtt
     1921 gtatcagtga ggagcatgca gagacggtcg ataccgattc ccaaaccacc tgttggtggc
     1981 ataccgtatt caagagcctc gatgtagtca tagtcgatgc ctgtcgcttc atcatcacca
     2041 agttctttag ctttagcttg ggcttcaaaa cggctaagtt gatcgattgg gtcgttcaac
     2101 tcagtaaagg cattaccgta ctccttagtc atgataaaga gctcgaaacg gtcagtaaag
     2161 cgttggtctt caggattttt cttagcgagt ggagatacag ctactggatg tccatagaca
     2221 aaggttggtt ggattaaagt ttcttcgaca aactcttcaa agaaggcatt gatgatgtga
     2281 ccaacctcag tgtagtgttt ctcaactgga actttcttct cagcagcgat agctttagct
     2341 tcttccaaag tcatgtcttg ccagaaatcg acaccagtaa tttctctgat agcatccacc
     2401 atatgaacac gcttaaatgg tttgttaatc ttgatttcag taccttggta gttgactggg
     2461 ccatcacctt tgactgattt agcagcgtgt tggataatgc cttccgtcaa gtccatgatg
     2521 tcttggaaat ctgcataagc ttggtaaact tcgatagaag taaactcagg gttatgagta
     2581 gcgtccattc cttcgttacg gaagatacgg ccaatttcat agacacgttc cataccaccc
     2641 acgataaggc gttttaagtg aagctcagtc gcgatacgaa gcaccatgtc aatgttttgg
     2701 gcattgtggt gggtgataaa tggacgggca gcagcaccac cggcttcatt atgaagaaca
     2761 ggtgtttcca cttcaaggaa tcctttttgg tcaaggtaac gacggatttc agagatgatt
     2821 tttgaacgag tgacaaagcg ttcaaagctt tcacgattag aaatcaagtc aaggtaacgt
     2881 ttacggtaaa ttgtttcaac gtctgtcaaa ccatggaatt tctcaggaag aggacgaaga
     2941 gccttagaca agtgtgtgat gtgggttgcc ttgatagaga gttctcccat atccgtacgc
     3001 atcacttcac cttcgacacc aaggaagtca ccaaggtctg cttttttgaa gatttcgtag
     3061 ttttcttcac cgacagcatc cttacgaaca tagatctgaa tctggccttc gcggtcttga
     3121 aggtgggcaa aaccaacttt tcctttacca cgtttggtta ccaagcgtcc tgcgatagta
     3181 gctgtttcgt ttttatcgtg taattgttct ttatcgaggt tggcatattt atcttttaat
     3241 tcttgtgaat ttgcagtacg ttcaaaacgt tttccgaaag gatcgattcc ttgttcgcgg
     3301 agcgcagcca ttttttcacg gcgaacgatc tgctggtcat ttagttcttc catatgttct
     3361 gtagacatgg gatcctccta gttttactca aaattgaata cgatgagtca ttttttgcga
     3421 tacccccatt ttatcataaa taagcaagaa attcacggat aatctttaaa aactaaaaag
     3481 aacctgacgc taaaatcact ggaaagaagt gaaagtatct tccgccccac tagcgatata
     3541 gccaaatgct gctttaggaa taacttgttg cgccattggc tccaaatcat aggtattgat
     3601 gaaatctaca tgaccttctg cattgcttgt tttgtatgac ataaaatgcc ctccttgata
     3661 agtaagcgtt tactttgtat attgcagttt ggggaagttt gggatccttt ctgctcggtt
     3721 taatcgttag tatcatccga cattatcgaa tccctgtttt ggcgcaagta gcgacagcct
     3781 acattgaatt gtcacgtaat acgccccttt tgattcaact cttctttctc tacttcggtc
     3841 ttccccgaat cgggattgtc ctatcttcag aagtctgtgc aacgcttggg cttgtctttt
     3901 taggaggctc cgccatgcag gattcgggaa tccaagtact ctttcaagga aataacctcc
     3961 tgagaatctt acagggattg ggcgttacga ttgggatatc catcctgtct gtcctcttat
     4021 ccatgatgtt cggaacagtc atgggaatca tcatgacctc ccattctaga atcatacgat
     4081 ttttaacacg attgtatctg gaatttatcc gtatcatgcc ccagctggtg ctactcttca
     4141 tcgtttactt tggcttggct cgaaacttta atatcaatat ctcaggtgag acttcagtta
     4201 ttatcgtttt taccctctgg ggaacagctg aaatgggaga cttggtacgt ggagctatca
     4261 cttctctccc taaacatcag tttgaaagtg gacaggcact cggcttgact aatgttcaac
     4321 tttactacca catcatcatc ccacaagtct tgagaagact gctaccgcag gctatcaatc
     4381 ttgtcactcg gatgattaaa accacttcat tagttgtttt gattggggtt gtggaagtga
     4441 ccaaagttgg acaacaaatc atcgatagca atcgcctgac catcccaact acttcatttt
     4501 ggatttatgg aaccattcta atcttatatt tcgcagtttg ctaccctatt tccaaactat
     4561 ccactcactt agaaaaacat tggagaaact aaatgtctga aactatctta gaaatcaagg
     4621 aactaaaaaa atccttcgga gacaatccca tcctccaagg actttctcta gaaatcaaaa
     4681 aaggggaagt tgttgtcatc ctagggccat ctggttgtgg gaaaagtacc ctccttcgtt
     4741 gcctcaacgg cttagaaagt attcaaggtg gagatattct tctggatggt cagtctatcg
     4801 ttgaaaataa aaaagatttt cacctagttc gccaaaagat tggcatggtc ttttaaagtt
     4861 atgaactctt tccccatctg gatgtcttac aaaacctcat cctaggccct atcaaaactc
     4921 aaggatggga caagaaagaa gtaacggaag aagctttgca attactagat cgtgtcggtt
     4981 tgctggataa acaacatagc tttgcccgtc aattatctgg tggacagaag caacgtgttg
     5041 caattgtccg tgccctccta atgcatccag aaatcatcct ttttgacgag gtgactgctt
     5101 cgctggatcc agaaatggtg cgtgaggtgc tggaacttat caatgatttg gcccaagaag
     5161 gccgtaccat gattttagta acccacgaaa tgcagtttgc ccaagccatt gctgaccgga
     5221 ttatcttcct cgaccaaggg aaaatcgctg aagaaggaac agctcaagcc ttctttacca
     5281 atccgcaaac caaacgagcc caggaatttt taaacgtctt tgactttagc caattcggct
     5341 catatctata aaggagattc ttatgaaact attcaaacca ctcttaactg ttttagcact
     5401 tgcctttgcc cttatcttta tcactgcttg tagctcaggt ggaaacgctg gttcatcctc
     5461 tggaaaaaca actgccaaag ctcgcactat cgatgaaatc aaaaaaagcg gtgaactgcg
     5521 aatcgccgtg tttggagata aaaaaccgtt tggctacgtt gacaatgatg gttcttacca
     5581 aggctacgat attgaactag ggaaccaact agctcaagac cttggtgtca aggttaaata
     5641 catttcagtc gatgctgcca accgtgcgga atacttgatt tcaaacaagg tagatattac
     5701 tcttgctaac tttacagtaa ctgacgaacg taagaaacaa gttgattttg cccttccata
     5761 tatgaaagtt tctctgggtg tcgtatcacc taagactggt ctcattacag acgtcaaaca
     5821 acttgaaggt aaaaccttaa ttgtcacaaa aggaacgact gctgagactt attttgaaaa
     5881 gaatcatcca gaaatcaaac tccaaaaata cgaccaatac agtgactctt accaagctct
     5941 tcttgacgga cgtggagatg ccttttcaac tgacaatacg gaagttctag cttgggcgct
     6001 tgaaaataaa ggatttgaag taggaattac ttccctcggt gatcccgata ccattgcggc
     6061 agcagttcaa aaaggcaacc aagaattgct agacttcatc aataaagata ttgaaaaatt
     6121 aggcaaggaa aacttcttcc acaaggccta tgaaaaaaca cttcacccaa cctacggtga
     6181 cgctgctaaa gcagatgacc tggttgttga aggtggccat taactcttaa aagggactgg
     6241 attttaagct ccaatccctt tttaagattt tacctataac atcctgagtc tatctaagat
     6301 gttcaatctg aacacagtgt acatacttta tcttctattg catatacttt atcacataag
     6361 atacgaatat cctcttcact atgactagca atcaaaattg ttgtcccttt ttcactagag
     6421 agctttctaa acaatgttct catattttct acacttgatt tatccaaggc attcataggt
     6481 tcatctagta aaagaataga gggattctcc ataattgctt gagcaatccc tagctttttc
     6541 ctcataccta gcgaataagt tttaactttc tggtcttttt gctcatatag accaactatt
     6601 ttcagtgtat cattgatttc ctgattacca actactcctc gtatgcttgc caaatattgt
     6661 aaattcttaa agccactata ataatttata aaaccaggtt cttcaatcaa agctcccaaa
     6721 ttagctggaa tttttctctc aggaacaata ttttccccat tgattaacac ttctccataa
     6781 gacggactat ataaaccagc tattaattta aacaatacac ttttccctga gccattcgca
     6841 ccagtgattc ctatgatttc ccctggttta caactaaagt taaggttttg aaaaacacat
     6901 gtctttttta atttcaactc aatatttttt aatatgatta tttcattcat tctataaacc
     6961 tcctcttttg acgagtgaaa tagaaaatac ttttaaaaag aaagactaaa aatagcaact
     7021 gaagaaataa atctcgtcct atatctccat tccctcgatt caaaatataa aatagataat
     7081 tagttcgatt ccctacaaat agaccaccaa acacaatcat gagtaaaaag aaactaacgc
     7141 aagcaaagtt catttgtaca ttcgctccta gtgcgtataa cataaagcaa agcaaggata
     7201 gcacacaagc attcaaagaa tcaactataa acaaaactcc tatatcccag tttggtaact
     7261 gtaacataaa ttgaatcact agaactttta ccatgattaa aatgagtcca taaagaaaac
     7321 aggtccaaat ctgataccta atccagagct taatagaaga aaatcgaatc acttccataa
     7381 agaaatcgga tgctcttcta cgatatacaa ctgctagaaa aaatgtatga ataataaacc
     7441 aatgaagcaa ccatatggat aagactaaaa aagagttttg actcagatta ggccctttta
     7501 aaatttctat aacaagtcta tcatttcctt ctaaccaata ccataatcct aaacaaatca
     7561 atatccaaaa aagaacataa ccccaaaagt acttggtata gattatctga ttcaacctcc
     7621 acatgtaact acttaacttt cccatttcca acttttacac tctcctccag tttctatgaa
     7681 cagaaaatac agtcaatgga attatgatag atagtaatgt aagccaataa atcaatgcat
     7741 acggattgga atttaccata tactgaaaaa ttggcaataa tccaattatc ccattgt
//
LOCUS       DAVLYR010000088         7741 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLYR010000088 DAVLYR010000000
VERSION     DAVLYR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.088"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..459
                     /locus_tag="VYO63_001411"
     CDS             <1..459
                     /locus_tag="VYO63_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672319.1"
                     /translation="RYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLK
                     HHYQIYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHY
                     SNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(494..916)
                     /gene="nusB"
                     /locus_tag="VYO63_001412"
     CDS             complement(494..916)
                     /gene="nusB"
                     /locus_tag="VYO63_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1672320.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREYT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(909..1298)
                     /locus_tag="VYO63_001413"
     CDS             complement(909..1298)
                     /locus_tag="VYO63_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1672321.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(1320..1880)
                     /gene="efp"
                     /locus_tag="VYO63_001414"
     CDS             complement(1320..1880)
                     /gene="efp"
                     /locus_tag="VYO63_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1672322.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2009..3451)
                     /gene="gatB"
                     /locus_tag="VYO63_001415"
     CDS             complement(2009..3451)
                     /gene="gatB"
                     /locus_tag="VYO63_001415"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1672323.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(3451..4917)
                     /gene="gatA"
                     /locus_tag="VYO63_001416"
     CDS             complement(3451..4917)
                     /gene="gatA"
                     /locus_tag="VYO63_001416"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1672324.1"
                     /translation="MTFNDKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDTDNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSASVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILSAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(4917..5219)
                     /gene="gatC"
                     /locus_tag="VYO63_001417"
     CDS             complement(4917..5219)
                     /gene="gatC"
                     /locus_tag="VYO63_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1672325.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5432..6976
                     /locus_tag="VYO63_001418"
     CDS             5432..6976
                     /locus_tag="VYO63_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1672326.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7231..7428
                     /locus_tag="VYO63_001419"
     CDS             7231..7428
                     /locus_tag="VYO63_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1672327.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            7539..7715
                     /locus_tag="VYO63_001420"
     CDS             7539..7715
                     /locus_tag="VYO63_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1672328.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
BASE COUNT         2175 a         1531 c         1762 g         2273 t
ORIGIN      
        1 cgttactgga aactcatcca acaggatagt cgtaaattga gcgataaacg tttttatcgc
       61 cctacttttc gcatgcactt aacaaataaa gaaattcttg acaagatttt aagctattca
      121 gaagacttga aacaccacta tcagatctat caactcttac tttttcactt tcagaataag
      181 gaaccggaga aatttttcgg actcattgag gacaatctga agcaggttca tcctattttt
      241 cagactgtct ttaaaacctt cctcaaagat aaagaaaaga ttgtcaacgc ccttcaacta
      301 cactattcta atgccaaact ggaagcgacc aataatctca tcaaacttat caagcgcaat
      361 gcctttggtt ttcggaactt tgaaaacttc aaaaaacgga tttttatcgc tttgaacatc
      421 aaaaaagaaa ggacgaaatt tgtcctttct cgagcttagc ttttcttcaa cccactacag
      481 ttgacaaaga gccttattgt tcttctgtta caaactggct gagcagtcca ttgataaaac
      541 gggcagattt ttgatcggag aagtcctttg caagctcgat agcttcatta acagcaacca
      601 gctgaggagt gtcaaatgaa gtgatttcaa agactcccaa gcgaaggagg tttctctcca
      661 cgagcgttaa gcgttcaatg gtccaacctg cttttaaatg ctgagtgatt tgcttatcta
      721 gttcttcctt tttagcttga acaccagaaa cgaggtctat caaaaaggct ggaagttgta
      781 catccgtata ttcacgatca tgagtatagg cgaaacgaca agcagtttcg acatccgtac
      841 cgaactcaag gctcatgaga gcttgaaaag cgcatttacg gagttggcgt ctagattcta
      901 atagtggact agtcattgag gaagtcctcg tcaaatagat ctttcaattc tggttttggt
      961 gttttatctg ggacgatacc tgcaacgtga atattgatag cagcgagttc tacatcagcc
     1021 atattacgga cggcatcttt gacagctttc tggatagcaa ccgctacctt aggaactttt
     1081 actccgtact caaggtagag atagatatct gctgtgagtt cttcgtccac gtttttaaga
     1141 taaatgccac ggccgagtga aagttttgaa agggtatcag acactgatct gtttgaaaaa
     1201 gagtgaacac cctctacctt tgcagtagcg atagcaatga ttttttcaag tacacgtggg
     1261 gcgataacga tttcgccaag ttgttcttca attcccatag aatgacctct ttctagagat
     1321 taggcacgag aaacgtaagt tccttctgca gtgttgataa cgagtttttg tcctgcttcg
     1381 atgaagtctg gaacgtttac gacaagtcca gtttccatcg ttgctggttt accagaacct
     1441 gtaacagtag cacctttgat agatggttga gtttcagcaa ctgtcaactc aacagtagta
     1501 ggaacggtga caccgatcac ttcagttccg tagaattgga ttttcacatc agagttttca
     1561 aggatgtaaa gcaattcgtt ttcaacattg actacaggga tttcgtattg gtcgtaagtt
     1621 tctgtattca tgaagtatgc tgtgtcatcc attttgtaca agtattgagc tgggacagtc
     1681 tcgataatag cttgttcaaa tttttcctct ggacggtagc ttgtgtcaaa tgtagaacca
     1741 gtacggacat cacgcaattt catacgcatg atcgtgtttc ctttacctgg tttgtggtga
     1801 ctagcttcca aaacgcgaat caatttgccg tcagctgttt caaaggtcat accagctttt
     1861 aatttacttg cttcaatcat gtttttacct ctttaataaa tattattact cttcatttta
     1921 ccataaaata ctctggaaat aaagccaaaa tagttattgg gagttggtag agaagaaaaa
     1981 accaacctta gggctggttt tgttctgtct agttttcttt caacttcgcc aattcctgtg
     2041 caagtagttt aagggcaact tgtgggttgg cttggccttt ggttgccttc ataaggaatc
     2101 ctgtaaaggc cttgtcggcg ttacgtttgc ctgacttgaa gtcggcaaca gcagcttcgt
     2161 tatcggcaaa gacttggtgg atgattggga tcaagatagc tggatctgaa atttgaacca
     2221 tacctgcttt ttccacgtat tcacgcgcgc caccgccatt tttagctaga tggacaaaga
     2281 ctttcttggc aatcttagat gaaatagtac cgtcttcgat gatggtaatc atttcaacca
     2341 agttttctgg tgtcaattcg atttgttcca gtgttttacc ttcagcattc aagaactgag
     2401 cgacttcccc ttggagccag ttagagactt gtttggcatc accacctagg gcaacagctt
     2461 tttcaaagaa gtcagaagtg actttattag cagtcaactg actagcatcg tagtctgata
     2521 aaccaaggtc agatacataa cgcgcacgac gttcttttgg aaactctggc aactcagtcc
     2581 gcatttcctc aatccactcg tcagaaattt caaagagggg taggtctggt tctgggaagt
     2641 agcggtagtc agcagcccct tccttgacac gcatgaggat ggttgttttg ttagcttcat
     2701 cgtaacggcg tgtttcttgg cggatttgac cacctgagcg aagaatttca gcctggcgtt
     2761 ggacttcgta ttcaagacct ttacgaacgt ttgagaagga gttgaggttc ttcaattcag
     2821 tcttggtacc gaatttttct tgaccataag gacgaaggga gatgttggca tccacacgca
     2881 tagaaccttc ttccatctta acgtcagaaa tgccagcgta ctggataact tccttgaggg
     2941 ctgtcagata agcataggct tcttcaggag aacgcatatc tgcctcagat acaatctcaa
     3001 tcaagggaac cccttggcgg ttgaggtcaa cataagagta gccatctgta ccatgggtgt
     3061 ttttaccagc gtcttcctct aagtgggcac gttcgatacc gattttcttg gtcgtaccgt
     3121 cttctagttt gacttcaatc cagccattat atccgattgg ttcatcaaat tgagaaattt
     3181 ggtaggcttt ggggttatca ggatagaagt agttcttgcg gtcaaagtgc atctttttgt
     3241 ggatgtccat gttgagggca agagcagcct tgataccggc atcaacaacc cctttattga
     3301 gaactggtag aactcctggg aaagaccagt caattacgtt agtgttggca ttttggtcat
     3361 ttccgaagtg ggcagaagta ggtgagaaga tttttgaatt ggtgttgagc tctacgtgga
     3421 cttcaagtcc gatgactgtt tcaaagttca ttagttgtca cctccaaaaa tcacgggttg
     3481 ttgtttgtgg tagtctgttg ttgcttcaaa agcagcagca gcttggtaaa tggtttcctc
     3541 agagtacttg ggaccaatca attggagtcc gacaggtaga ccttgagaga atccagcagg
     3601 aatcgaaatt ccaggcagtc ctgccaagtt tacaggtatg gtcaagaggt cggctaagta
     3661 catggcaact gggtcatggt tgagagaatc caagtcatag gcaacacttg gagcagttgg
     3721 acccaaaatc aaatcgtaat ccgcgaagac tttttcgaaa tcttgaatga taagtgtacg
     3781 gacctgtcca gccttcttgt agtaggcatc gtagtaacct gatgaaagac tgaaagtacc
     3841 cagcatgata cgacgtttta cctcttcacc aaaaccttgg cttcggctgt ttacatagat
     3901 ttcatcaagg ttggttgcat cttctgcgcg atagccgtaa cggataccgt caaagcgttg
     3961 caagtttgat gaagcttctg atgaagcgat gatgtagtat acggcaactc cgtatttaga
     4021 gtgaggaagg ctgacttctt cgacaatagc accaagtttt tcaaagtgtt tagcggcact
     4081 tagaatggtt tccttaacct ctgggttaat tccttcaccg aggtattcct taggcaaagc
     4141 gattttcata cccttgatgt cttggccgat ttttgaagta aagtcggcga tgcggacaga
     4201 agcagaagta gagtctttag catcttcgct ggcaatagcg ttgagcaaga gggcattttc
     4261 cttaacagta ggagcaaaag gtccaatctg gtctaatgag ctgccaaagg caatgagacc
     4321 gaaacgtgaa actgttccgt aggttggttt gagaccaacg attccgttga aggcagcagg
     4381 ttggcggatg gaaccaccag tatcagaacc aagtgacaag cgaacttgtc ctgaggctac
     4441 agctgcggca gaaccacttg atgacccacc aggaaccttg ctgtggtccc aagcgttttt
     4501 agttgctccg tagtgtgaag tttcacctga accacccata gcaaattcgt ccatgttggt
     4561 ctttccaacg acaatcatgc ccttggtttt tgcattggca acagctgtcg catcaaagat
     4621 tggctcatag ttgtagagca tttttgaggc agcagttgtg agaataccgt ctgtagagat
     4681 gttatcctta acagcaagtg gaattcctga aaggacattg tcagtatcaa ttccagcttc
     4741 atcaatggct ttagcttgaa caagagcttg ctcctcagcg atggtgacaa atgaattgag
     4801 ggcttcctca cgagacttga tattttcaag tgttgcttgg gtcaattctg ttgcagaaat
     4861 ttccttagag acaaggagat tgtgcaactc ttcaatagtt ttatcgttaa aagtcattag
     4921 gcatctcctc catcgtctag gatagctggc accttgatat agtagttgtc tttttcaggt
     4981 acgtttttaa acaagcgatc acggtctgtt ccttcttcgg ccacatcagg gcggagtaca
     5041 gtcttgcggt cagccatagt cgtagtaggt gcgacaccag ttgtgtcaac ttcgcccagc
     5101 aattcaacca tgtcaacaat cttagacaag gtggtcgcaa aggcagcagt ttcttcttca
     5161 gagaatctta attttgaaag attggcaacg tgtgttacct cttcttgcgt aattttcatc
     5221 ttgcatcctt tcgtgaaatg atgattttta gtctgttcta ttttaccata ttttcctata
     5281 aataagggag ggggaagtga aaagaaatag aaattgaaaa aaatgctaaa atccgatata
     5341 atggagtgtt gaaaggaatg gtaaaatcca atgtaaaaat cattctcagc tattgaaaca
     5401 agaaaaatag agaatcaaag aaagagaact tatgaatatt caagaagaaa ttaagaaacg
     5461 tcgtaccttt gccattatct cccacccgga cgcggggaaa acaaccatca ctgagcagtt
     5521 actctacttt gggggtgaga ttcgtgaggc tggtacggta aaagggaaga aaacagggac
     5581 ttttgctaaa tctgactgga tggatatcga gaagcaacgt gggatttctg ttacttcatc
     5641 tgttatgcaa tttgactacg acggcaagcg cgtgaatatc ttagacacgc cagggcacga
     5701 ggacttctca gaagatacct atcgtacctt gatggcggtg gatgctgcgg tcatggtcgt
     5761 ggactctgcc aaggggatcg aggctcaaac caagaaattg tttgaggttg tgaaacatcg
     5821 tggcattcca gtctttacct ttatgaacaa gctggaccgt gacggtcgtg agcctttgga
     5881 tctcttgcaa gaattggaag aaatcttggg cattgctagc taccctatga actggcctat
     5941 cgggatgggg aaagcctttg agggcttgta tgacctctat aaccaacgtt tagagcttta
     6001 taaaggggat gagcgttttg ccagtctaga agacggggac aaactctttg gtagcaatcc
     6061 tttctacgag caagtcaagg atgacattga gcttttaaat gaagctggga atgagttttc
     6121 agaggaagct attctggctg gagaattgac gcctgtcttt ttcggttcag ccctgacaaa
     6181 ctttggtgtg cagaccttcc ttgaaatctt cctcaagttt gctccagaac cacatggtca
     6241 caagaaaaca gacggtgaaa ttgtggatcc ttatgacaag gatttctcag gctttgtctt
     6301 taaaatccaa gccaacatgg atcctcgtca ccgtgaccgt attgcctttg tccgtatcgt
     6361 atcaggcgaa tttgagcgcg gcatgagtgt caatctccct cgtactggta agggggctaa
     6421 actatctaat gttacccagt ttatggcgga gagtcgtgag aatgtgacca atgccgtggc
     6481 aggtgatatt atcggggttt acgataccgg tacttatcag gttggggata ccttgacggt
     6541 tggaaaaaac aagtttgaat ttgaaccact gccaaccttt actcctgaaa ttttcatgaa
     6601 agtttctgct aagaatgtta tgaagcaaaa atccttccac aaggggattg agcaattggt
     6661 gcaagaagga gccgttcagc tttataagaa ttaccaaaca ggtgagtaca tgctgggagc
     6721 tgttggtcaa ctccagtttg aagtctttaa acaccgtatg gaaggcgaat acaatgcgga
     6781 agtggtcatg agcccaatgg gtaaaaagac cgttcgttgg atcaagcctg aggacttgga
     6841 tgaacggatg tcgtcaagtc gcaatatctt agccaaagac cgttttgacc aaccagtctt
     6901 tctctttgaa aatgactttg ccctccgctg gtttgcggac aagtatccag acgtagagtt
     6961 agaggagaag atgtgactca gtaccaacaa ttggaactaa agttccaaat tgttggacgc
     7021 tagtcgctat ttggcgaact agctaactgc ctcactaact acgtttggca aatgaaatcg
     7081 atttgtaaaa ctccgtgtcg taatgtaaaa attgtaaacc gtaatgggac ttcatctaaa
     7141 atctccactg gtatggtttt acgaacgaat aggcgaaaat aatggttacg tagaaacata
     7201 tgatgggaat aaaaagaaat gagataaaaa gtgaaagaag agaaaaaagc gattgtctta
     7261 ggtgcagata atgcttatat ggataaagtt gaaacaacaa taaaatctct ttgtgttcat
     7321 cattataatc ttaagtttta tgtttttaat gatgaccttc cgcgagaatg gttccaattg
     7381 atggaaaaaa gactagagac tttgaatagt gagattgtga atgtttagat tgacagtaat
     7441 attctgaaag gttatcgtct cccctttgag ggactttcat atgcggcttt tttaggtatt
     7501 ttattccaaa gtatgtaagc gagtctcgtg tactgtattt ggattcagat attgttgtaa
     7561 gaaaatcgat agatgagctg tgggatttgg acttgacggc tataccctta gctgcagtta
     7621 gagatgattt ttatacacat aattttaatt ctggtgttct gttaatcaac gatggcatgt
     7681 ggagagcaga aaatgtcacg caagacctga tttaagacac aaataaaaaa tcttccaatt
     7741 c
//
LOCUS       DAVLYR010000089         7682 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLYR010000089 DAVLYR010000000
VERSION     DAVLYR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.089"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            16..387
                     /gene="rnpA"
                     /locus_tag="VYO63_001421"
     CDS             16..387
                     /gene="rnpA"
                     /locus_tag="VYO63_001421"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1672329.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            362..1186
                     /locus_tag="VYO63_001422"
     CDS             362..1186
                     /locus_tag="VYO63_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1672330.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            1205..2191
                     /locus_tag="VYO63_001423"
     CDS             1205..2191
                     /locus_tag="VYO63_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1672331.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            2242..2865
                     /locus_tag="VYO63_001424"
     CDS             2242..2865
                     /locus_tag="VYO63_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1672332.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLEKQAKILDIPTVKGIYYLAYSDGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            2996..4453
                     /locus_tag="VYO63_001425"
     CDS             2996..4453
                     /locus_tag="VYO63_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1672333.1"
                     /translation="MEVISSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            4476..4757
                     /locus_tag="VYO63_001426"
     CDS             4476..4757
                     /locus_tag="VYO63_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1672334.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            4834..5319
                     /locus_tag="VYO63_001427"
     CDS             4834..5319
                     /locus_tag="VYO63_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1672335.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            5336..6001
                     /locus_tag="VYO63_001428"
     CDS             5336..6001
                     /locus_tag="VYO63_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1672336.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            6005..6868
                     /locus_tag="VYO63_001429"
     CDS             6005..6868
                     /locus_tag="VYO63_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1672337.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            6870..7574
                     /locus_tag="VYO63_001430"
     CDS             6870..7574
                     /locus_tag="VYO63_001430"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1672338.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIIVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
BASE COUNT         2368 a         1354 c         1732 g         2228 t
ORIGIN      
        1 agatggagtt gagttttgaa gaaaaacttt cgtgtaaaaa gagagaaaga ttttaaggcg
       61 attttcaagg aggggacaag ttttgctaat cgcaaatttg tggtctacca attagaaaac
      121 cagaaaaacc attttcgagt aggtctatca gttagcaaaa aactggggaa tgccgtcact
      181 agaaatcaaa ttaagcgacg gattcggcat attatccaga atgcaaaagg gagtctggta
      241 gaagatgtcg actttgttgt cattgctcga aaaggagtcg aaaccttggg atacgcagag
      301 atggagaaaa atctactcca tgtattaaaa ttatcaaaga tttaccggga aggaaatggg
      361 agtgaaaaag aaactaaagt tgactagttt gctaggactg tctctgttaa tcatgacagc
      421 ctgtgcgact aatggggtaa ctagcgatat tacagccgaa tcggctgatt tttggagtaa
      481 attggtttac ttctttgcgg aaatcattcg ctttttatcg tttgatatta gtatcggagt
      541 ggggattatt ctctttacgg tcttgattcg tacagtcctc ttgccagtct ttcaggtgca
      601 aatggtggct tctaggaaaa tgcaggaagc tcagccacgc attaaggcgc ttcgagaaca
      661 atatccaggt cgagatatgg aaagcagaac caaactagag caggaaatgc gtaaagtatt
      721 taaagaaatg ggtgtcagac agtcagactc tctttggccg attttgattc agatgccggt
      781 tattttggcc ctgttccaag ccctatcaag agttgacttt ttaaagacag gtcatttctt
      841 atggattaac cttggtagtg tggatacaac ccttgttctt ccgattttag cagcagtatt
      901 caccttttta agtacttggt tgtccaacaa agctttgtct gagcgaaatg gcgctacgac
      961 tgcgatgatg tatgggattc cagtcttgat ttttatcttt gcagtttatg cgccaggtgg
     1021 agtcgcccta tactggacag tgtctaatgc ttatcaagtc ttgcaaacct atttcttgaa
     1081 taatccattc aagattatcg cagagcgcga ggccgtagta caggcacaaa aagatttgga
     1141 aaatagaaaa agaaaagcca agaaaaaggc tcagaaaacg aaataaataa ggaggaatct
     1201 ggtaatggta gtatttacag gttcaactgt tgaagaagca atccagaaag gattgaaaga
     1261 attagatatt ccaagaatga aggctcatat caaagtcatt tctagggaga aaaaaggctt
     1321 tcttggtcta tttggtaaaa aaccagccca agtggatatt gaagcgatta gtgaaacgac
     1381 tgttgtcaaa gcaaatcaac aggtagtaaa aggcgttccg aaaaaaatca atgatttgaa
     1441 cgagcctgtg aagacggtta gtgaagaaac cgttgacctt ggtcatgtag ttgatgctat
     1501 taaaaaaata gaggaagaag gtcaaggtat ttctgatgaa gtcaaggctg aaatcttaaa
     1561 acatgaaaga catgccagca ctatcttaga agaaactggt cacattgaga ttttaaatga
     1621 acttcaaatc gaggaagcga tgagggaaga agcaggcgct gatgaccttg aaactgagca
     1681 agaccaagct gaaagtcaag aactagaaga cttgggcttg aaagttgaaa cgaactttga
     1741 tattgaacaa gtagctacgg aagtaatggc ttatgttcaa acgattattg atgacatgga
     1801 tgttgaggct acactttcaa atgattataa ccgtcgtagc atcaatttgc aaattgatac
     1861 taacgaacca ggtcgtatta tcggctacca tggtaaagtc ttgaaggcct tgcaactgtt
     1921 ggctcaaaat tatctttaca actgctattc aagaaccttc tatatcacta ttaatgtcaa
     1981 tgattatgtt gaacaccgtg cagaagtctt gcagacctat gcgcaaaaat tggcgattcg
     2041 tgttttagaa gaaggacgca gtcatcaaac agatccaatg tcgaatagtg aacgcaagat
     2101 tatccaccgt attatttcac gtatggatgg cgtgaccagt tactctgaag gtgatgagcc
     2161 aaatcgctat gtcgttgtag atacagaata agtaaaatca gggttgacct gattttttgc
     2221 tagctagagg aggttaaact gatgttgaat aagataagag actatctaga cttttctggt
     2281 ttgcagtacc gtagtcctga taaagcggga gcagagcgag agaagatgct ggcattccgt
     2341 cacaaaggac aagaggcccg aaaggctttt acagaactgg ccaaagcctt tcaagcaagc
     2401 catccagaat ggcaactcca acagactagt cagtggatga atcaggccca gcgtttgaga
     2461 ccacattttt gggtttatct acagagagac ggacaagtga cagaacctat gatggcctta
     2521 cgtttgtatg ggacatctac tgactttgga atttctttgg aagtcagttt catcgaacgt
     2581 aagaaggatg agcaaacttt agagaaacag gccaagattt tagacattcc aaccgttaaa
     2641 ggaatttatt atctagccta ctctgatggc caaagtcaac ggtgggaggc gaacgaagaa
     2701 aagcgtcgta ctttacgcga gaaggtgaga agtcaagaag ttcgaaaagt tttagtgaag
     2761 gtagatgttc ctatgacaga aaattcgtct gaagaagaaa tcgtagaagg cttattgaag
     2821 tcttattcta aaattcttcc ctattatcta gctacgagaa aataagataa tttgtaaaac
     2881 atcataaatc atacagtcca agagtgaaca gtccgctgtg taattcttgg tctttttgtt
     2941 tgcgctttcg cattatataa taaacttaca aaaacaattc aaaaggagaa caattatgga
     3001 agtcatttca agtgttctaa attggttttc tagcaatatt ttgcagaatc ccgcattttt
     3061 cgtaggttta ttggtgttga taggatatgc acttttgaaa aaacctgccc atgacgtttt
     3121 ttcagggttt gttaaagcaa cagtagggta tatgttgctt aacgtgggtg ctggtggttt
     3181 ggttacaacc tttcgtccaa tcttagcagc tcttaactac aaattccaaa ttggtgcagc
     3241 ggttatcgac ccttactttg gacttgctgc agcaaacaac aaaattgcag cagagtttcc
     3301 agattttgtt ggaactgcaa ctacagctct attgattggt tttggaataa atatcttgct
     3361 cgtagctctt cgaaagatta cgaaggtaag aaccctcttt attactggtc acatcatggt
     3421 acaacaagct gcaacagtat ctcttatggt tctattctta gtaccacaat tgcgcaatgc
     3481 ttacggtaca gcagcgattg gtatcatctg tggactttac tgggcagtta gttcaaatat
     3541 gactgttgag gcaactcaac gcttgactgg tggtggcgga tttgcgattg gtcaccaaca
     3601 gcaatttgca atctggtttg tagataaagt agcaggacgc tttggtaaga aagaagaaag
     3661 tttagacaat cttaaattac ctaagttcct ctcaatcttc cacgatacag ttgttgcatc
     3721 tgctaccttg atgctcgtat tcttcggagc cattctttta atcttgggtc cagacattat
     3781 gtctaataaa gaagtcatca cttcaggaac tctattcaat cctgctaaac aagatttctt
     3841 tatgtacatt atccaaacag cctttacctt ctcagtttac ttgttcgttt tgatgcaagg
     3901 tgtccgaatg ttcgtatctg agttgacaaa cgccttccaa ggtatttcaa acaaattgtt
     3961 gccaggttca ttcccagcgg ttgacgttgc agcttcttat ggatttggtt ctccaaatgc
     4021 tgtcctgtca ggatttatct ttggtttgat tggtcaattg attacaattg ttttgctcat
     4081 cgtctttaaa aatccgattc ttattattac aggatttgta ccagtgttct ttgacaatgc
     4141 agccattgcg gtctacgctg ataaacgcgg cggatggaaa gcggctgtta tcctttcctt
     4201 tatatcaggt gtccttcaag ttgctctagg agctctttgt gtggcccttc tcgatttggc
     4261 atcttatggt ggctaccatg gaaatatcga ctttgaattc ccatggcttg gatttggata
     4321 tatcttcaaa taccttggta ttgttggtta tgtacttgtg tgtctcttct tgcttgttat
     4381 tcctcaactt caatttgcca aagcaaaaga taaagagaaa tattacaacg gtgaagttca
     4441 agaagaagct tagtatctag aaaaggagaa ataaaatggt taaagtatta gcagcgtgcg
     4501 gaaatggaat gggttcatca atggttatca agatgaaggt tgaaaatgct ctccgtaagc
     4561 ttaatcaaac agattttaca gtcaattcat gcagtgtcgg tgaagctaaa ggtttagcag
     4621 taggatatga catcgtaatc gcttctcttc atttgattca agaattggaa gggcgaacta
     4681 atgggaagtt aattgggctt gataacttga tggatgataa agaaatcacc gaaaaactca
     4741 gtcaagcact acagtaaaag gttggagggg gctggacaga aactgagagt tatcgtttct
     4801 gtccttctcc ctctttaaat aaaggaggca gatatgaatt taaaacaagc tttaattgac
     4861 aacgactcga tccgactagg tttagaggct aacgattgga aagaagcagt caaggtagca
     4921 gtagatccct tgattgaaag tggggcaatt ttgccagagt attacgatgc tatcattgaa
     4981 tcgactgaag agtatgggcc ttactatatc ttgatgccag gtatggctat gccccacgct
     5041 agacctgaag ctggtgtgca aagtgatgcc ttttcattaa ttaccttaca aaatcctgtt
     5101 gtattttcag atgggaaaga ggtatctgtt ttgttggcac tagcagcaac aagttcaaaa
     5161 attcacacaa gtgtagccat tccacaaatt attgccctgt ttgaattaga agattctatt
     5221 gcacgtttac aggcttgcca gactaaagaa gatgtcttgg ctatgattga agaatctaag
     5281 gatagccctt atctcgaagg attggatttg gaaagttaga aagaggaata aagaaatgac
     5341 aaaaagaata cctaatttac aagttgcatt agaccattca gacttgcaag gagcgattaa
     5401 agcagctgtt tctgttggtc aggaagtaga tattatcgaa gctggaactg tttgcttgct
     5461 tcaagttgga agtgaactgg ctgaagtctt gcgtagcctt ttcccagata agattattgt
     5521 ggcagacaca aaatgtgctg atgctggtgg aacagttgct aaaaataatg cggttcgtgg
     5581 agcagactgg atgacttgta tctgttgtgc aaccatccct actatggaag cagctctaaa
     5641 ggctatcaag actgaacgag gagaacgagg cgaaatccag atcgagcttt atggcgattg
     5701 gacttttgaa caagctcagc tttggctaga tgcaggtatc tcacaagcta tttatcacca
     5761 atctcgtgat gctcttcttg ctggtgaaac ttggggtgaa aaagacctta ataaggttaa
     5821 aaaactcatt gacatgggct tccgtgtatc tgtaacaggt ggtctagatg tagatactct
     5881 caaactcttt gaaggtgttg atgtctttac ctttatcgca ggtcgtggaa ttacagaggc
     5941 tgtggatcca gcaggagcag cgcgtgcctt caaggatgaa atcaaacgaa tttgggggta
     6001 aatcatggta cgtccaattg gaatttatga aaaggcaacc ccaacacact ttacttggct
     6061 agaacgttta aattttgcca aggagttagg ctttgatttt atcgagatgt ctattgacga
     6121 acgtgacgag cgtttagcaa gacttgactg gagtaaggaa gaacgcttgg aagttgtcaa
     6181 agaaatctat gaaactggtg ttcgtattcc ttctatctgt ttttcaggcc atcgtcgcta
     6241 cccattggga tcaaaagatt cagttctaga ggaaaaatct ctagaactca tgaaaaaatg
     6301 tatcgaatta gctcaagact tgggagttcg tacgattcaa ttagctggtt acgatgttta
     6361 ctatgaggaa aagtcacccc agacacgcca acgttttatc aaaaatttga gaaaagcctg
     6421 tgactgggct gaagaagctc aggtggtact tgctattgaa attatggatg atcctttcat
     6481 cagtagcatc gaaaaatatt tggctataga aaaagagatt gactctccct tcctctttgt
     6541 atatccagat attggtaatg tgtctgcatg gcataatgat atctatagtg agttttatct
     6601 tggtcatcat gccatcgcag ctctccatct caaggatact tatgcagtga cagaaagttc
     6661 aaagggccag ttccgagatg tacctttcgg gcaaggttgt gtcaaatggg aagaagcttt
     6721 cgatatttta aaggaaacca attataatgg acctttccta atcgaaatgt ggtctgaaaa
     6781 ttgtgaaaca gtagaagaaa cacgcgcagc cattcaagag gcgcaagctt ttctctatcc
     6841 actcattaag aaagcaggtt tgatgtaaga tgaatcaagt aatcaatgct atgcgtaaac
     6901 gagtctgtga tgccaatcaa tcattgccaa aacatggact tgtcaaattt acctggggga
     6961 atgtatctga agttaatcgc gaactcggtg tcattgttat caaaccatca ggcgtggatt
     7021 atgacgaatt gacacctgaa aacatggtag tgactgatct agatggtaag atcctagaag
     7081 gggatttaag accatcttcc gacctcccaa ctcatgtgca attatataag gcttggtcag
     7141 aaattggtag tgtggttcac acccattcga cagaagctgt tggttgggct caggcaggtc
     7201 gtgatattcc tttctacgga acaacccatg cagattattt ctacggttca atcccttgcg
     7261 cccgtagttt gaccaaggac gaagtagaag tggcctatga aaaagatact ggcctggtta
     7321 tcgtagaaga gtttgaacat cgcggactta acccggttga agtaccagga attattgtac
     7381 gcaatcacgg tccattcacc tggggcaaaa atccagagaa tgctgtttat cactctgtcg
     7441 tactagagga agtatcaaag atgaatcgct ttacagaaca aatcaatcca agagttggac
     7501 ctgctcccca gtacatacta gaaaaacact accaacgtaa acatggacca aatgcttatt
     7561 atggtcaaaa gtaagaacga tgaaggagga gaaaaagata aatttagctc ctctttttac
     7621 atttgatttt tattgagagt aaagttggaa ttgaagtaat tttaaaagat tttttagaaa
     7681 ta
//
LOCUS       DAVLYR010000090         7464 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLYR010000090 DAVLYR010000000
VERSION     DAVLYR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.090"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(613..1833)
                     /gene="divIB"
                     /locus_tag="VYO63_001431"
     CDS             complement(613..1833)
                     /gene="divIB"
                     /locus_tag="VYO63_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1672339.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQKDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            complement(1843..2901)
                     /locus_tag="VYO63_001432"
     CDS             complement(1843..2901)
                     /locus_tag="VYO63_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1672340.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(2905..4257)
                     /locus_tag="VYO63_001433"
     CDS             complement(2905..4257)
                     /locus_tag="VYO63_001433"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1672341.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(4361..5002)
                     /locus_tag="VYO63_001434"
     CDS             complement(4361..5002)
                     /locus_tag="VYO63_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672342.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(5004..7025)
                     /locus_tag="VYO63_001435"
     CDS             complement(5004..7025)
                     /locus_tag="VYO63_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1672343.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLIDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(7096..7395)
                     /locus_tag="VYO63_001436"
                     /pseudo
     CDS             complement(7096..7395)
                     /locus_tag="VYO63_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         2230 a         1647 c         1289 g         2298 t
ORIGIN      
        1 agtaggtaat atcttaatct tatcaacaat tctcataaca aacctaacct ttaataaata
       61 aaagaggggc taagtaaaac atactctagt ccctcagtcc gcaatcaata aaataatgac
      121 taattctatt aatctcttgc tgcaagacta atacggaaaa ttcaaatgac tgtctaaatt
      181 gaaaaagctt gatattagta agtgaacaaa cttcaacaac cagcaagagc aaggattgct
      241 aaaccaaatc cgatacctgt tgcaagtcca gctgaaaaat cactaactgg tactgaaatt
      301 tcttcaattt cctcaaataa aaagttatct tgcatgatta tcactcccta tctttttaac
      361 aacaggctac tccaattaag agaactaccc cagttccaag taaaactccc ccccaaaatt
      421 caccatctgt aggggctacg atttcttcaa tttcttcaaa taagaatact tcatctgaca
      481 tatgtgttac cttcttcata aaaaattttt ttgtaatcga ttacattgca aatgtaacat
      541 aaagaaaaac agatgtcaaa tattaaacgt aaaaacatgg caactaaaga gctataggag
      601 aaaaggtaaa acctagcgac gcgatgagcg ctgggtcgtt tggtttcgat tgctctcttc
      661 ctcttgtttt ttctgttctt cttctaaacg tttacgttcc tcttcctgcc ttttctcagc
      721 ttcttcggcc tcttttttag ctttttcttc tgcttccata atcaatttat ccgctacagt
      781 gtagctgtag actccagctt ccatgtcaat cacactcggt tctgacaatt gtggcttaat
      841 cttactgtaa tatggcagtt tcttactcat ttcagataga ggaaccaaga cttcgtccga
      901 atcattcatg gtcaatcgaa ttaaatcgga tgtcaccttg cttggggcta attccacctt
      961 ttggatagcc gccttgagtt ctgggctaat ttgagcaagt tctgagacaa aaaccttgat
     1021 ttgttcacta tcattaaaga gaactgataa ataagtttct ggtaaactgt tcagactcac
     1081 agaactagtc tcaagctgac cactggaaag aataggataa tgattttcac cagaaatata
     1141 gtaggccaca atatcatatt ccttgacctt aatagtgaac ttagttggaa attgatagac
     1201 aagttgagct gattcaaccc aatagttaga cttaatctgc ttttcatatt ttgccttgtc
     1261 tagcagaagg ttaatcgtat aatccgaatc ctgaatgcct gaagcctgtc gaatatcatc
     1321 agctgtagtt tgcaccgttc cctcaacacg aatatctttc atggtcgcat aaggactgag
     1381 caagtaggca gagacaatca ataaaagcag acttggaaat aaaatcgtga aggctcgcaa
     1441 gatatggata ccaggaatct ttgctttggc tggtttttcc tttgtagcct ttttagcaag
     1501 ctttttatcc tgttcctcct tctctttaga ctctggttct tctttctctt ctttctcttt
     1561 gtcagcctct gaggatgcta ctttttcttc agactcttcc ttagctgatt ctgaatcttt
     1621 ctggtctgtt tcactctcct ggtcctgttt atcctctgac ttctcagatt cttctcccat
     1681 tcgagcttgt ctttcctttt ccttctcctc agctagagcc gcctcttctt cagccttctt
     1741 ttttagatat tcttggtttc gtttctgcca ttctgataac tctttcaatt cttcgagggt
     1801 ttctttgtcc tcatttttct tatcttttga catttacttt ccttatgata aatctttttt
     1861 caacaattga taaaaatctg ctagagattt caattcctta gaagctttca tcttagcttg
     1921 gtaatcttcc ttgtgactta gtaagtgaga aagcttctct tccaaactat ccaaggtcaa
     1981 atcgctttct tgaaggtctt ctgcatagcc tttcttaaca aagtaagctg cattttcaat
     2041 ctggtcacca cgactagctt cacgaccaag cggcacaatg acatgcaatt ttgctatcgc
     2101 caagagctca aaaatcgtat tggcaccacc acgtgtcaca acaatatcag ccaattccat
     2161 caagggttga tagagatcgg tcacatagtc aacacgaaaa agattttggc tcaactcatt
     2221 caggctagaa tctccagtta gattgataat attgtagcgc tctgttagtt cttttttatg
     2281 gtctgtcacc aattggttaa agacacgagc acctgcagaa ccgccaacaa acaatacagt
     2341 cggcaattta tgattaaagt ggctttgaat atccaccaat tcatctggtt ctggattttt
     2401 ttgatctgaa accttggtca ctgctcccac atgctcaacc ttagccaaac tcgaagcttg
     2461 ttcaaaggtt gaatacatct tagtcgcaaa tttataggcg attttattgg ccaagcccat
     2521 agacaggtca gattcgtgaa taaagacagg cactcctgac acacgcgcag cgataacagg
     2581 cggtactgag acaaagcccc cctttgaaaa aagggtctgt ggacgaagtc gcaacatgat
     2641 aaagagcgat tggacgattc cccagccaac tttgaagacg tccagcatat tttgccaaga
     2701 gaaatagcga cgcaatttcc cagtcgcaat ggagtggaaa gtgacatcca aacctgactt
     2761 aaggatttct tggtgttcga taccacgctt gtccccgata tagtggactt cccaaccatc
     2821 ttcgatgaac ttgggcatta acaaaagatt gagggtaacg tgtccaaccg tccccccacc
     2881 tgtaaagaca atttttttca tattttattc ttttaactcc gctactgtgt cgataaagag
     2941 gtcgccacgt acttcaaagt tagcatacat atcccagctg gcattggcag gactaagaag
     3001 aaccacatct ccttgagtcg caagctcata ggccttgcgg gtcgcatctg caatatctgt
     3061 cgcctccaca taagcgacac cagccttgtc tgctgcccgt ttgacacgtt ctgcagattg
     3121 acccaggatg accatcttct tgagtccagt aatatctggc accaattcgt caaactcatt
     3181 gccacggtcc aaaccacctg caatcaagac gaccttgctg ttgtcaaatc ctgacaaggc
     3241 tttttgagta gccaagatat tagttgattt actgtcgtta tagaatttaa cacccttgat
     3301 gtcatccaca aactggagac ggtgtttgac accaccgaag gctgaaagag tttccttgat
     3361 ggtttgattg tccacaccac gaagcttggc tacagcaata gtcgcaaggg cattttccac
     3421 attgtggcta cctggaacac cgatttcatt cgctgccatg actacttcac cacggaagta
     3481 gagttgacca tcttccagat aagctccatc aaccttttca agtgttgaaa atggtacaac
     3541 agtggcttct gtcttggaag tcaagtcttt tgccaagtct tgattaaagt tcaagacaag
     3601 gaaatcagct gctgtcatct tgttctggat attccacttg gctgctacat attcctcaaa
     3661 taatccatga taatcgatat gagttggcat aaggttagta ataaccgcaa tctctggatg
     3721 aaattcttga acacccatga gttggaaaga agaaagttcc ataacaagcg tgtccttgtc
     3781 cgacgcagtt tgggccactt gactagctgg atagccgata ttccctgata aaagaccatg
     3841 ttgcccagca gcagtcaaaa cttccccaat catagtcgtt gtggttgtct taccgttcga
     3901 tcctgtgata ccaataatcg gtgcttctga aatcaaataa gccaattcca cctcagtcaa
     3961 gactggaatt cccttggcca aagccttttc aatcatggga ttgttgtagg ggatacctgg
     4021 atttttcacc ataagggcaa actcttcatc caagagttcc aaaggatggc cacctgtaat
     4081 gaccttgatc ccttcttcca gcaaactttg ggcagctgga ttgtcctcga atggtttccc
     4141 gtcatttact gtcactatgg cgcctagctt atccaacaaa cgagctgcag attcaccaga
     4201 cttggccaaa cctaaaacaa gaactttctt atttttaaat tgatctatta ctttcatgtc
     4261 tcgaactcca tttctactcc tactatttta ccatttttat ggaaataaaa aagccacaaa
     4321 gtgtgtttgt gactctttct tctaactgaa tcttaccata ttatctatgt gataaatcgg
     4381 taactcgaat gacctgatcc acttgctccc aaatcagagg attgtgggtc gcaataataa
     4441 tggtccgatt cggatttttt aaagattcta ggatggaaag taattcctca gagtttttgg
     4501 ggtctaagga agcggttggt tcatctgcaa gaatcaaagg cggatccttt aaaattatct
     4561 tcgctaatgc aacacgttgt gcttctcctc ctgataactc aaatataggt tgcttcaaat
     4621 ccaaataaga gaggtttaca cggtttagag cttgtttcat caaagagatt ttctcttttt
     4681 ccttcaactt tttaccaact aaacccagat tgagattctc tttgacggtt tggctttcaa
     4741 ttaagccaaa atcttgaaat aagtatccca agtaatctct aaagaaaaca gaaggcttga
     4801 tgtccttaag agaagtgcca tcatagatga tttgcccttt gtcatatggc tctaatcgtc
     4861 caatcatatt caagagtgtt gtcttaccac agccacttgt accgattaag gcataaattt
     4921 tcccaccttc aaaatgaaga tttgtatctg aaaatagctg acggcttcca aattttttag
     4981 atatattctt tagttcaatc atcctatttt cctttcataa ttgtcataga aacacgagat
     5041 tctttctgcg cttgacggta aagcgtcaga actgcactag ctagaaagac taataaagtg
     5101 agcaagccaa tcaccaagtc tcgactgctt aaaataaaga gactagcacc aaatacaaaa
     5161 ctggcaaatt gactaaccat atactgagca tgtgtttcaa aaaatcgtaa acctgaaatt
     5221 cgtttaatca aaatatctcg gcggaattgc tcgaaatata gaagattgac agaataaaat
     5281 agtaacaagg aactagctat ccccacaata gctcctaaga ttaaagttgc tgtttcagtt
     5341 tgaacttcat tataacgagt tagataaaca cttcttcctt ctttaagata ggatacttgc
     5401 tcataaattc cagctttctt caagagttct agcccactct catatccttt gataaagagt
     5461 tgttttccag cattgataga ccaactagat aaggatataa aactatcacc tgtagaagtc
     5521 ggcgtgaata caactaaaat cggatcaatc aaatactgag tggatacggg attctcaccg
     5581 ttattataaa caaaccgctt ttctcccatt gaaagataac taacgtgcgc tttcatctca
     5641 taatccaaag gagcacttgc ctcctcacca gattttccat aataactcaa tctttcttca
     5701 aaaactttct taagttctgc ttctcgagag cgcaaatgtt ctgggagcaa gaggataaac
     5761 tcaccttttt ggagatgggc taacttctgt ttggtctcag catctaccac gaccttttcc
     5821 ttgtccaaat aactgggact aacatagagc gtattagcat ctggactata ggtatccagt
     5881 gtctctccct gttcattttt tccttgtgga ttggcaaaat ggagcagatt atcctttaca
     5941 taaagagctt gttcttcttc gattgcttcc ttggcaaagg cataccactt gctctgattt
     6001 tctgtatctt ttcctctatc acctaagcca aaagaaattt gataatagtc tgctctatcc
     6061 tgccatgctt gttttgaaat ttcaagttct ttcaatcgtt ggtaagacgt caaacctgtc
     6121 ttaacagcgt atcctactgt aaagacagca accaactgac acaatagggt taaaatcatc
     6181 aggcgtttaa ggggtaatct ccccttaata acgggaacta atgctttgta attcaaactc
     6241 attagataaa gaagcattag taaaattgaa atcgccaata aaaacaacag atagaaacta
     6301 atcccaaaac cataggtggc taacaagata ggataaaaca aaccttgact aaaaagaacg
     6361 actcccccac ctaggaagga aaggagggct gatagaagga gccatttgat atcagtagat
     6421 aaagaatgcc ccatgatgga taagagagtc tgaccagaaa agagttttat acctgctgct
     6481 ctcatttcct taatccgagt gataatcact aaagcaaaga aagataaacc aaatattgct
     6541 aaactaatta aaataagggg atttagtaat attcgaaaag caagaaaata gggcggtatc
     6601 tttcggtcag cacttgcttt ataacccaaa tctcctaatt tatcggcaag cttttctttc
     6661 gtcaaggagc ctgacaaaag gagataacta tttagcggat cactacgttc acgactttct
     6721 tggctagctt cttggaattc ttttggtaaa gttccctgac cataagttgc ataagtaaag
     6781 tgagtcgtcc catccttact cggctctaca attcttctag ctattaaact ctgttctgag
     6841 tttgcaaaat tctccaattc ctgttcaaat acctcacgcg tcggttcctg agtatctttt
     6901 ttgacacgaa gtaaagaaac ggaatcatag cttgcatata aatattgtgg cgcacgtaag
     6961 acaataatcc aagcaaggaa gaagctgaga aaaaaagttg ataataatat gaatagtttc
     7021 ttcatagtag actccttgta aacaaaattc cccctgtaat ttcttacaag gggaacgatt
     7081 taaatcaatg aacgattagt cataatcaca gtaaaatgct acttgttctc cccatttagt
     7141 ccaaatccat gcataggagg tttttccagc aggagcttct cctttatcag aatgaccatc
     7201 ccatctgctc acgatggatg aataatgata ttttttacca tgatagtaat ttgaaaaagc
     7261 ctaaccacct cctgaacctt ctccatatgt ccatactcct ccatctggat attgtacagc
     7321 agctgatgca gctcccaata atgtaaaatt tgaaataaga gctagagcaa gtaatctatg
     7381 ttttttcgtt ttcattttat tttttctttc aaaaaaatca caccttgagc aacaatgcaa
     7441 caaaataaat cctcctctct cttt
//
LOCUS       DAVLYR010000091         7102 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLYR010000091 DAVLYR010000000
VERSION     DAVLYR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.091"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..846)
                     /locus_tag="VYO63_001437"
     CDS             complement(1..846)
                     /locus_tag="VYO63_001437"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1672344.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKACAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            1102..2129
                     /locus_tag="VYO63_001438"
                     /pseudo
     CDS             1102..2129
                     /locus_tag="VYO63_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2430..4319)
                     /locus_tag="VYO63_001439"
     CDS             complement(2430..4319)
                     /locus_tag="VYO63_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1672345.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGTEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKEQSKQLLTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLLGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            4457..6022
                     /locus_tag="VYO63_001440"
     CDS             4457..6022
                     /locus_tag="VYO63_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1672346.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYANAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            6022..6762
                     /locus_tag="VYO63_001441"
     CDS             6022..6762
                     /locus_tag="VYO63_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1672347.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(6871..7086)
                     /locus_tag="VYO63_001442"
     CDS             complement(6871..7086)
                     /locus_tag="VYO63_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672348.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         2231 a         1606 c         1294 g         1971 t
ORIGIN      
        1 ttatacaaat aaacggacaa aactgtaaag tagcaaaaca ctaccaagca cgatacggta
       61 tttaccaaaa agggtgaagt cgtgtttttt cacatagctg gtcaagaagc gaatagccac
      121 catgctgacc gcaaaagcta ctcccatcgc gaccaagagc aaaaacaatt gcccaaagct
      181 caagagttct ccggctttca caaatttgaa aatctttaag gcactagctc caaacataac
      241 gggaatccca agatagaagg taaattctgt cacaactgaa cgactggttc catttaacaa
      301 accaccgaca atcgttgcac ctgaacggct agtccctggt aaaagagcaa gaacttggaa
      361 gagtccgata tagaaagcgg tcgtataagg aagcttgtcc aactctgtta cacttggctc
      421 gatagcacac gctttattgc gcttttccaa atagatgaag gcaaccccgt agataatcaa
      481 catgagagca actgaaacca tgttatggaa gtgggtatca aaccaatcat caaatttaaa
      541 gacaccaagt aaaggtaaag tagcaatcaa gaccttcaac catagtctcc aagtcttacg
      601 aacttcctgt ttgtccttag tcggtttaaa aggattgagc ttgttaaaat aaatcaccat
      661 aactgctaaa atagcaccaa gctgaatcac gacattaaac atggacataa aggcttcatt
      721 ttgattttgg tattggataa actcctctgc taaaatcaag tgacctgtac tggaaatcgg
      781 caaccattcc gtaattcctt caacaatccc gaagaagata gattttaaaa tttcaataag
      841 atacatatat ttctccttat attatattta acgcagttag ctactgccaa agcccaagtg
      901 gtatacttgg aataagccac tgtggattag ttcatttcct ccaatcgctt gacgatttcc
      961 gaaaggatct aaccacagct ttttgtttca attggtaatg agtttgataa cgcatagacg
     1021 ttttatctcg tctaaggtaa caaggcaaaa agttcgtgga taaccaagta gacttgaaat
     1081 actatatctg gaggtactcc catgctttat gtgggcattg atgtcgctaa aaataaacac
     1141 gatgttacag ccttgaatgt tcgaggaaaa ctgttcttaa accactcact ttttcaaata
     1201 ataaagctgg ttttgaacta ttagatctgt ctcttcgaca gctcaaccaa gactgtctca
     1261 tcgctcttga agataccgga cattatgcct ttaatctact gaacttcctg catgaacaag
     1321 gatataaagt ttacacctac actcctttgc tcatcaagga atttgccaaa tcattatcac
     1381 ttcgcaagac taaaacggat aagaaagacg ctcatggcat cgctcttaaa cttctttctg
     1441 accccaatcg tgaacaattt caacacgata atcggcaagt agaactaaaa atactggcta
     1501 gacatattca tcgtctcaag aaaaaacagt ctgattggaa agtacaatac actcgttgtc
     1561 ttgatatcat ctttcctgag ttggataaaa tcgttggaaa gcattcagaa tatacctacc
     1621 aactcttgac atgctaccct aatcctcaga aaaggcttga ggcaggattt gataagctga
     1681 tagaaattaa gcgattgacc gcttctaaaa ttcaggatat cctctcagtc gcacctcgtt
     1741 ctatcggaac aacatctcct gctcgtgaat tcgaaatcat cgaaaacatc aaacattaca
     1801 agaggctcat tgacaaggcg gaaaaatgtg tcaatgactt gatggctgag ttcaactcgg
     1861 tcatcacgac ggttactggg attgggaatc gtttaggggc ggtcatttta gccgagattc
     1921 aaaatattca tgcctttgat aatcctgctc aattacaagc tttcgctgga ctggattctt
     1981 ctatttatca gtcaggtcag attgatttag ctggaagaat ggtcaaacgg ggttctcctc
     2041 atctgcggtg ggcactcata caagctgcca aagcatgcgc tcgcttttca cctgctttta
     2101 aggcctatct taagactaag ttagaataag aaaaacatta caatgtagcc atcatccacc
     2161 ttgcaaaaaa acttatccga accctgtttt atatcctaaa aaagagctgc catttgacga
     2221 acaaaaagtg atgtaatttc tgaaatcaac acaggattac ctttgaaaaa atggactttt
     2281 tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc atcaaattga aataagaaat
     2341 agtttagata aaattattga cttttcttat agtttgtctt tcttctatct tccattatat
     2401 catacttttt cagtctatgg tagctctctt taaaaggctc cgatactgcc gccacctccg
     2461 cctccagaga agccaccgcc agaacttcca cttccagaag atacggagta ggtacttgct
     2521 gtatttgcga cactagcata atggctcatt tgcgcgcttg aatgataaaa catactgtgc
     2581 cagccataag ctacatagag attgatatct ggattttcaa cttgaatctg atgaaccttc
     2641 atcaaatgac taaccttgtc cgcatagcca agtaaggttg catagaccaa gagacgattc
     2701 caaacaacaa tactttccaa ttcagcctta tccagtcgtg caatctcacg taacatattt
     2761 tcaaaactgg tccagagata gtagacctca gctcccgctt catttagaac accatcacga
     2821 ttatctagtc gaagcttcca ataatagaaa acagacaaaa ctaaccctag aaaaccaagt
     2881 attggcaaag ggaggtaaag atagccatga acgtctaaac tgtacaagaa caaaccaaat
     2941 ccgataaata ggggcaggat agtcaaggca cccataccca cttgcaaggc cttttcccca
     3001 ccagttaaag gacgataata atctgggagc ccccagaagg aaactcgttt tctcactcct
     3061 tcttgcatct ggttcaatac ctcttcaaaa gaagatttga gttgaagccc tcttgcttga
     3121 atccgttttt catcagaaac tttggctcta cgataaagac tatcagatac cttgtaatcc
     3181 gcaaacaaat tggaaagagt ttcttctttt ttacctgaaa aggctagatt tagacagtct
     3241 ctctcaaagc ttgacaaacc atcttctttt actagcctca aaccaactgc atctccttct
     3301 gaaatgatag agacattccc acggtctatc acatctagca aggtagcttg aataagttga
     3361 tcaaaggtga attttccagc tcccttgacc aagggactca cttcctccaa ggaggtcgag
     3421 tagactgctt ctgataaaac cataggctct aattccattg gtggttcata gagacgatga
     3481 tttttggcat atttgactga aggagtggtc tttcttctat aaataaaata gaagcagaca
     3541 ctcaataaca aggagatgga aaggatcgaa gggaggaccc aagtaagtag ttgtttactt
     3601 tgctcttttt ctctaacaat cgagtcttct atcttattaa actcttctaa tcgattccct
     3661 ttcaatccct gatccctagc gctagcaaaa tcggtccgag gccaataggc atgcaactca
     3721 actccacgct tagccggaag attgtctaaa cggatagtat aatcaaggtt actcttttca
     3781 atcgttccct ctctaaaaag tttccctgta tggaaaaaga gtttttcagt ccccttgtct
     3841 ccccttacat gaaattcaaa cttttcaata gactctgaac tatctgtcag aggttgccaa
     3901 tttaattcag cgatatcatc ataaaggaaa agtaaatttt ttaagttcca gacgaggtca
     3961 acttcaacta tgtcgccctc ctgacctgga ttatagactc tcacagtata accatccgct
     4021 tcttctgtta cttcgctagt cacatctgct agttctgcac cgtttttcgc ggcctgaatc
     4081 tttggatgag ggtcaatgtc aaacccgcta ggcatcttac cagcacgtcc aagtcccacg
     4141 atttggccct taaagtcctc ctcaaactgg taaactatct tctgtctaaa ctctgccgta
     4201 ttatctgcat gaatatacaa atccccttga taagagttta tcttgaaatc aatggcaaaa
     4261 acagagagtg gaagaaggca aaacaagcct aacaccagta agaaaaaagt ttttttcatc
     4321 aactaagccc cctttttatc tttcttatca ttatagcatt ttttcgtaag taaaggctta
     4381 cttatgttga aaaattacgc atttttcgat aaaataggag acagttattt tttaatagat
     4441 cagaatagga gaaatcatga gaaaaatata cttatctatt ttcacaagtc tcttgctgat
     4501 gctaggactt gtcaatgttg ctcaagccga tgaatattta cgcatcggga tggaagcagc
     4561 atatgctccc tttaactgga cccaggatga tgatagcaac ggagctgtca aaatcgatgg
     4621 gaccaatcag tatgccaacg gatacgatgt tcaaatcgcc aagaaaatcg ctaaggactt
     4681 aggtaaagaa cctttggttg ttaaaaccaa gtgggaaggt ctagtccctg cccttacttc
     4741 tggtaagatt gacatgatta tcgcaggtat gagtccaact gcagaacgca aacaagaaat
     4801 tgccttttcg agcagttact acactagcga accagttcta ctggtcaaaa aagattctgc
     4861 ctatgcaaat gctaaatctt tggatgactt taatggtgcg aaaatcactt ctcaacaagg
     4921 tgtctacctt tatgacttga ttgcacaaat cacaggtgct aaaaaagaaa cagccatggg
     4981 agacttcgct caaatgcgcc aagctcttga ggctggtgtc atcgacgcct atgtttctga
     5041 acgcccagaa gctctgactg ccgaagccgc taactctaag ttcaagatgg tccaagtaga
     5101 accaggtttc aaaactgggg aagaagatac agctatcgcc attggacttc gtaaagatga
     5161 cactcgtatt agccaaatca atgccagcat tgaaaccatt tcaaaggacg accaagttgc
     5221 cctgatggat cgtatgatca aggaacaacc tgccgaagct acaacaactg aagagactag
     5281 cagtagtttc tttagccaag ttgctaaaat tctttctgaa aactggcaac aactcttgcg
     5341 tggtgctggt atcactcttt taatctctat cgtcggaacc atcacaggtc tcattattgg
     5401 acttgccatt ggtgttttcc gtactgctcc tctctctgaa aacaaagcca tttacggcct
     5461 acaaaaacta gtcggctgga ttctcaacgt ctacattgaa attttccgtg gtacgccaat
     5521 gattgttcaa tcgatggtta tctactatgg aactgcccaa gctttcggga tcaaccttga
     5581 ccgtacactg gctgctatct tcatcgtttc aatcaatacc ggtgcctaca tgactgaaat
     5641 cgtccgtggt ggtatcctag cagttgacaa gggacaattt gaagctgcga ctgctcttgg
     5701 tatgacccat aaccagacca tgcgtaagat tgtcctacct caggtagtcc gtaacatcct
     5761 acctgcaact ggtaatgaat ttgtcatcaa tatcaaagat acatctgtat tgaacgttat
     5821 ctctgttgtc gaactttatt tctcaggaaa taccgtggca acacaaacct atcaatactt
     5881 ccagacattt acaatcatcg ccgtgattta ctttgtcctc accttcaccg taacacgtat
     5941 cctacgattt atcgagcgcc gaatggacat ggatacctat actacaggtg ctaaccaaat
     6001 gcaaacggag gatttgaaat aatgacacaa gcaatccttg aaattaaaca cctcaaaaaa
     6061 tcctatggac aaaacgaagt gctaaaagac atttcactca ctgtccacaa gggagaggtc
     6121 atctctatca tcggaagctc tggaagcgga aaatcgacct tcctacgctc cattaaccta
     6181 cttgaaacac caactgatgg acaaatcctt tatcatggac aaaacgtcct cgaaaaaggc
     6241 tatgacctca cgcaataccg tgaaaagttg gggatggttt tccaatcctt taacctcttt
     6301 gaaaatctca atgttcttga aaacacaatc gtcgctcaga caactgtcct aaaacgcgaa
     6361 cgcacagaag ctgaaaagat tgccaaagaa aacctggaaa aggtcggcat gggagaacgc
     6421 tactggcaag ccaaaccaaa acaactctca ggtggtcaaa aacaacgtgt ggccatcgct
     6481 cgtgccctct ccatgaatcc ggacgctatt ctctttgatg aaccaacatc agctctcgat
     6541 ccagaaatgg ttggagaagt cctcaaaatc atgcaggacc tggctcagga aggcttgacc
     6601 atgattgtcg taacccatga aatggaattt gcccgtgatg tctctcaccg tgttatcttt
     6661 atggataagg gcgtgatcgc tgaagaaggt aaaccagaag acctcttcac caatcctaaa
     6721 gaagaccgaa caaaagagtt ccttcaacgc tatctcaaat aaaaagaaaa ggctgcatca
     6781 accgtgcagt ctttttgctg tcctcaaaat gaaaaggcag attctctatc tctactactt
     6841 gaaggtcaag aatccgccgt tatccaaaga ttaatcttca aatttttcat aatttttttc
     6901 atagaaatca attaggccta gggctgtttc aaatgaaata ttttttactt ttgcacgacc
     6961 ctgcgccaga gcaatgatag acatttcacg cgcattcgtt tctttagaga tacggtagcc
     7021 tgtgattttc ttatcacgaa cccagccaac aactgattct actttttcaa agtttgactt
     7081 agccatgttt ttctcctatt tt
//
LOCUS       DAVLYR010000092         7099 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLYR010000092 DAVLYR010000000
VERSION     DAVLYR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.092"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..185
                     /locus_tag="VYO63_001443"
     CDS             <1..185
                     /locus_tag="VYO63_001443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672349.1"
                     /translation="LGTDCSKHRFKVVDRTDEVSSKHCFEVVDRTDEVSNHTHDKPTL
                     TWFEEIFEEYHSPFHN"
     gene            complement(507..1643)
                     /gene="dnaJ"
                     /locus_tag="VYO63_001444"
     CDS             complement(507..1643)
                     /gene="dnaJ"
                     /locus_tag="VYO63_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1672350.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1848..2102)
                     /locus_tag="VYO63_001445"
     CDS             complement(1848..2102)
                     /locus_tag="VYO63_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672351.1"
                     /translation="MLVTVERNGNVIYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHIC
                     QELHLYKTQDPHYNKFEFDLAKRSLKITTRDNTTIFISNE"
     gene            complement(2215..4038)
                     /gene="dnaK"
                     /locus_tag="VYO63_001446"
     CDS             complement(2215..4038)
                     /gene="dnaK"
                     /locus_tag="VYO63_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1672352.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4518..5042)
                     /gene="grpE"
                     /locus_tag="VYO63_001447"
     CDS             complement(4518..5042)
                     /gene="grpE"
                     /locus_tag="VYO63_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1672353.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(5069..6103)
                     /gene="hrcA"
                     /locus_tag="VYO63_001448"
     CDS             complement(5069..6103)
                     /gene="hrcA"
                     /locus_tag="VYO63_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1672354.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(6267..6704)
                     /locus_tag="VYO63_001449"
     CDS             complement(6267..6704)
                     /locus_tag="VYO63_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1672355.1"
                     /translation="MHLKGWRYRTSRFGLFYFDQCQPDDVIYRIYDSRFLKKYKHELQ
                     DFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMSKGLKWEVMRSRLRSCTTTFLGGLV
                     VCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFRLKRKYRVDE"
BASE COUNT         1978 a         1583 c         1350 g         2188 t
ORIGIN      
        1 agctaggcac agactgctca aaacaccgtt ttaaggttgt ggatagaact gacgaagtca
       61 gctcaaaaca ctgttttgag gttgtggata gaactgacga agtcagtaac catacccatg
      121 acaagccgac gctgacgtgg tttgaagaaa tttttgaaga gtatcattca ccatttcaca
      181 actaaatcga agactttctg ccgtaatgaa aacacctgaa acagcttggt ttcagctgtc
      241 cggaaacttt aagacttagg ttcaaagttt aggtatggaa cttcgaagga ggtcgctacc
      301 gtccgtcatt acttaggaaa gtcttaaaaa atctataaag taaaaagagc ccgacagctc
      361 tttttgttta tactcaatga aaatcaaaaa gcaaactagg aaactagcca cgggctgctc
      421 aaaacactgt tttgaggttg tagatacgac tcacatatat acggcaaggc aacgctgacg
      481 tggtttgaag agattttcga agagtattat tctccatcaa aggcatcttt tatatggtca
      541 aagaagcctt ttttctttgg atttactttc aagtcaccag cagtcgcgaa ttctttcaag
      601 gctacttttt ggcggtcgtt caagcctgtc ggtgttacga cattaacagt aacgtattgg
      661 tcaccaactg caccgccacg aaggctcggt gcccccttac tacgtaggcg gaatttctta
      721 ccagtctgag ttccctctgg aataaccaat tcaacatcac cgtgaacagt tggaatatct
      781 actgtatcac caagagccgc ttggacaaag ttgaggttga gattgtagaa gatagtcgtt
      841 ccttcacgtt caaacttgtc gctagcttct acagaaacta ctacatacaa gtcaccataa
      901 ggtccaccgt taaagcctgc ttcaccttga ccagcgaggc gaatttgttg acctgtttcc
      961 acaccagcag ggattttcac atgtacgcta tgagcttgtt tctcatgacc tgttccatga
     1021 caggttgtac atggatattt gatttctttt cctcgaccgt gacagacatc acaggttact
     1081 tggcgacgca tcataccaag aggagtctgc gtatcgacgt taatgacacc agcgccatga
     1141 cagcgtccac aagtgactgg acttgtccct ggcttagcac cagagccatt acatgtacga
     1201 cagccagctt cacgatgata cttaacttcc ttctcagttc cgaagatagc ttcttcaaag
     1261 gtcaaattga cacgatactg gagatcatct ccttggcgag gagcgtttgg attgcgcgaa
     1321 gaaccgcctc cgccgaagaa acttgagaaa atatcctcaa aaccaccgaa gccacctgcc
     1381 ccattgaaac caccgaaacc accagctcca ccaaaaccac cattggcgcc tgcagcacca
     1441 tactggtcat aggcagcacg tttttggtcg tcactcaaag tctcataggc ttcttgaact
     1501 tccttgtact tgtcctcagc accaggctcc ttgttgatat ctgggtgata ttttttggaa
     1561 agcttacgat aagccttttt gatttcgtct gccgaagcgt ttttggatac ccccagacga
     1621 tcataaaatt cagtattgtt catacaagat accaagaccg taaaattcga ctgaaaaata
     1681 ggaaatctga cgctggagcg acgctcctag acagatttct cttttttccg agaatttagg
     1741 tcgggttcaa ttcctttctt ttatattgat gagataatac tccagccact cggccctatc
     1801 cctttttacc gaaaaacaga ggctgggttg caacctctgt ggttagatta ttcatttgaa
     1861 ataaaaatgg ttgtattatc tctagtagta atcttcaaag accttttagc taaatcaaac
     1921 tcaaatttat tatagtgtgg gtcttgtgtt ttatataggt gtaactcctg acatatatga
     1981 tccgaaaagt aaatcataac atctttaaac acagaagtgt ggtcatattt actcttacca
     2041 acagctactt ctgtaaattc tttataaatg acatttccat ttctttccac agtcaccagc
     2101 atttctgttg gattataatc caaattaaat aattattcaa aaaaacttta tcttttcatg
     2161 ataatctgca atgtcagaaa gattattttt attaacactc ataacactag ccccttattt
     2221 ttcagtaaac tctccgtcta cgacgtcatc gcctgcgttt cctgttgctt gtgcgccttc
     2281 tgctccttct tgagcttgtt gcgctgctgc ggcttgttcg tagagtttca cagcaaggcc
     2341 ttgagctttt tcgttcaacg cttcaagttt cgctttcatg tcgtccaagt tgttgtcttc
     2401 ttgagctttc ttaaggtcat caagggcagc ttgggcagcg tcacgttctg cgtcgaagcc
     2461 tttaccttca gtttccttga ttgtcttttc agtcgcaaag attgcttggt ctacttcatt
     2521 acgaaggtct acttcttctt tacgtttctt atcggcttca gcgtttgctt ctgcatcttt
     2581 catcatgcgg tcgatttctt cgtcagtcaa acctgagttc gattggatga caatagtttg
     2641 ttctttttga gttccaaggt ctttggcctt aacagacacg ataccgttct tgtcgatgtc
     2701 aaatgttact tcgatttgag gaattccacg aggtgcagct gggatatcag tcaattggaa
     2761 gcgtccaaga gtcttgttat ctgctgccat tgggcgttca ccttgaagaa cgtggatatc
     2821 aacggctggt tggttgtctg ctgctgttga gaagacttgt gatttagatg ttgggattgt
     2881 agtgttgcga tcgataagtt ttgtaaatac tccacccatt gtttcgatac caagtgacaa
     2941 tggcgttaca tcaagaagga caacgtcctt gacatcacca gtaatcacac caccttggat
     3001 agccgcaccc atagcaacta cttcatcagg gtttactgat ttgtttggtt ctttaccagt
     3061 ttcagcttta acagcttcaa caacggcagg gatacgagtt gaaccaccaa caaggataac
     3121 ttcgtcgatt tctgacaagc tcaaacctgc atctgaaagg gcttgacgaa ctggaacttt
     3181 tgtacgttca acaaggtcac gagtcaaatc atcaaatttc gcacgagtca aagtcatttc
     3241 caagtgaaga ggtccagcct cacctgcagt gataaatggc aagctgattt gtgttgaagt
     3301 tacaccagaa aggtctttct tcgctttttc agccgcatct ttcaaacgtt gcattgccat
     3361 cttgtcagta gacaagtcga taccgttttc tttcttgaat tctgctacca agtggtcaat
     3421 gattttttgg tcaaagtcgt caccaccaag tttgttgtcc cctgcagttg acaatacgtc
     3481 gaagacaccg tcacccaatt caaggataga gacgtcgaat gtaccaccac caaggtcaaa
     3541 taccaagatt ttttcttctt tgtcagtctt gtccaaacca taagcaagag ctgctgcagt
     3601 tggttcgtta acaatacgtt ctacttcaag accagcaatt ttaccagcat cttttgttgc
     3661 ttgacgttga gcgtcgttga agtaagccgg aactgtgata acagctttgg ttactttctc
     3721 accaaggtag tcttcagcgt agcctttcaa gtattgaagg atcatagctg agatttcttg
     3781 tggagtgtat tcttttccat ttgcagaaac tttttcagaa gttcccatct tagatttgat
     3841 agagataact gtatctgggt ttgtgactgc ttgacgtttt gcagcatcac caacgatgat
     3901 ttctccgttt ttgaatgaga ctacagatgg agttgtgcgg tttccttctg ggtttgcgat
     3961 gattttgctt tcagttcctt caagaactgc aactgctgag tttgttgtac ctaagtcaat
     4021 accgataatt ttagacatgt gtttttctcc ttgttagtta attttctatt tttctttttg
     4081 atactcttct taagtggcga cgccgtcaga gcttgacttc gtcaatctct ttgactaaac
     4141 tttgagccta aggtctcaaa atttgcgcaa atagcaccac ggcgaagagt atcgtctttg
     4201 gttcgcttcg ctcactatta caaagccaaa catattttat ctttcttcat agtttattgt
     4261 cgtttcggac aagttttcta ttatgtaaat tgcgacacga ggagtcgaaa tcgattttat
     4321 ttcgacgacg agttagtaag gaagctaggc aaacgtcata gcgattgccg ttttctgacg
     4381 agttgcttta gctaccgtca gaattgccga atcgaacacg ccctaagctc tgagtaaaaa
     4441 agatagattt tcttgtgttc atcgaacact tcgtcaatct cctattttta ctgcgagctt
     4501 tttacgagct ttgtatctta gttatacacc actaccattg ctgggcgtag gatgcggtca
     4561 tggagtttgt agcctttttg aaagacttgg gcgatggtat ctactgggtg ttcatcgtct
     4621 gctgggagag tttggatggc catatggtag ttatggtcaa attcgccatc tgctgcgatt
     4681 tcttcaattc cttcttcttt caaagcgtga atcaagcttt cttgcaccat cgccaagccc
     4741 ttcttcacat catctgtcaa accttcaact gcaagtgcac gctcaaggtt gtcaagagat
     4801 ggtaagattg cttttgccaa gtcctggcta cgataacgtt gcaagttttg acgttcttca
     4861 ttggcacggc gttggatatt ttgcatttct gcatgagcgc gaagatattt gttttcgaac
     4921 tcatctgcac gttcatttgc caagtccaac tcagactttt caggagttgt ttcttcagct
     4981 gtttccacaa cttcctcttc ttgaacttct tctacttctt cattttttat atcttgggcc
     5041 atgttcgcct cctttaatga tttcaatctt aatgtacttc gtaatgatta ctgctgaggt
     5101 agcggtaaaa atctgtcaac ttcatggtca aaacacggtt gaccacattg acttgattga
     5161 ttagctgttg gtaatccaga ttaactggac cgataatggc tagaattcca actcctcgat
     5221 aaggaatgag gaacttacta ctaatcaccg ctaggtcagc taaacaggac tcttgaccgt
     5281 ctgcaacacg aacattttgc atctgatcct cacgcaaccc ctcacgaatc tccaaggcca
     5341 ccttttgcgg ttggtcaaag aactgatagg cttctagatt ggcaaaattc aagagattga
     5401 ccttgcccgc catcacaatg ttttcgttga acatttcctt aaagatgtgt tcaaagagat
     5461 cgatgacatt atccgttgtt gtaaagtaac gctggataat ctgcggaatc tccgtccgaa
     5521 tcttgtagtg aatatctaaa acggtgtgac cgaggaaacg ttcctgaatg atgctcttca
     5581 gtttcagcaa atcctcctgc aagaagttcc ttggaatcag aaactgacta gtaaccgttc
     5641 gcgactcgtc tagggtaaat accgccaagg ctgtatgttg ccccaaaaca acgatatcaa
     5701 aggctgtcaa acgttgcctg ctcggctcaa catccagtgc cactaccgta cagccactca
     5761 ggtctgttag caagttagca gcctcttgca gaatatcctc caatttgaag aattcctgat
     5821 caaaggcttt gacaatctca tatacctcat tttcagccag ccggtgaaaa tccagtgagt
     5881 gtttcacata gtactgaaaa ccagcaacac ttggcatccg accgcttgaa gtatgagcct
     5941 tctcaagcaa cccttgtttt tctagttccg ccatgtcatt acgaatggtt gcactgctag
     6001 agttaataga ctcttgcaag gcttttgatc cgacaggttc gtgcgttttg gtaaagatgt
     6061 caataatcag atttaaaata tcctgctgac gctctgtaac catctcatca ctcctctctg
     6121 tctgatcaat tagcactcaa tgcatccaag tgctaactta tgattctatt atacaccctt
     6181 aaaagagaat gtcaagacaa aaactcaaaa aattagcact cttttatcaa gagtgctaaa
     6241 aatcaatctg aagacctaga accttgctat tcatctactc gatatttcct ttttagtctg
     6301 aaataaccat gaattagata agaaatcaga aaagcatata aaacaaaaat aagaaagaaa
     6361 gacatagaaa gatcctctct aaacaaactc gtacaaacaa caagaccacc taagaaagta
     6421 gttgtacagg aacgaagtct agatcgcata acttcccatt tgagcccctt actcatatag
     6481 acttgatcct ctggaagtaa atcagaagac gatttacgaa gaatcgaaca agaacctgct
     6541 cctatcaatt cccaacctct atctctaaaa tcttgcagtt catgcttata ttttttaaga
     6601 aatctagaat catagatacg gtagatgaca tcgtctggtt gacattggtc aaaatagaac
     6661 aaaccaaaac gactcgttct atacctccaa cctttcaaat gcatctcatg taaatattct
     6721 tcttacttgt ccaaatcaac aatggtgaaa atccgaaatt ctactctgct attcattgtc
     6781 ttaccccaaa attagaaaac atgcctggcg ttatttatta gataattctt tccacttttg
     6841 actcaatctc caaaaaatat aagaaatctg aatcgcaaaa actatcaata aaacccaatc
     6901 tattatgaaa atcaaaaaca ctttccaact gaaagaacta cctccagtga caaactttga
     6961 gaaaaacggt agtagagcta aaaagagaag taaaatagga agcatccgca ttgttaaaat
     7021 ccgtttggca taaaaaaatc tttatttaaa ctaaaatatt atatcaaaat ttacgccagt
     7081 ttttgaacgg ctgatgtag
//
LOCUS       DAVLYR010000093         6950 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLYR010000093 DAVLYR010000000
VERSION     DAVLYR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.093"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..143)
                     /locus_tag="VYO63_001450"
     CDS             complement(<1..143)
                     /locus_tag="VYO63_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1672356.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     GKEA"
     gene            307..459
                     /gene="rpmG"
                     /locus_tag="VYO63_001451"
     CDS             307..459
                     /gene="rpmG"
                     /locus_tag="VYO63_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1672357.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            469..645
                     /gene="secE"
                     /locus_tag="VYO63_001452"
     CDS             469..645
                     /gene="secE"
                     /locus_tag="VYO63_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1672358.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            700..1236
                     /gene="nusG"
                     /locus_tag="VYO63_001453"
     CDS             700..1236
                     /gene="nusG"
                     /locus_tag="VYO63_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1672359.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            1358..1837
                     /locus_tag="VYO63_001454"
     CDS             1358..1837
                     /locus_tag="VYO63_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1672360.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGELSHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            1931..2002
                     /locus_tag="VYO63_001455"
     tRNA            1931..2002
                     /locus_tag="VYO63_001455"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1964..1966,aa:Glu,seq:ttc)
     gene            2254..3799
                     /locus_tag="VYO63_001456"
     rRNA            2254..3799
                     /locus_tag="VYO63_001456"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            3851..3923
                     /locus_tag="VYO63_001457"
     tRNA            3851..3923
                     /locus_tag="VYO63_001457"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3884..3886,aa:Ala,seq:tgc)
     gene            4048..6949
                     /locus_tag="VYO63_001458"
     rRNA            4048..6949
                     /locus_tag="VYO63_001458"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2081 a         1303 c         1879 g         1687 t
ORIGIN      
        1 gcttcttttc ctttctgttg ataggctagt aacagacgat tgatttgtct ttcagaaaga
       61 ttgagttcga cacaggcccg tttctttgtt ttctttcctt gggctatagc ttttatcaca
      121 agatattttt tcgtttcatt catattcagt tggatccttt tcatatgact attctaccaa
      181 atgggacatt ttcacgttcg atttactaaa gacattatca cattcgaatc acaatagtca
      241 atctatttcg ctaagatgct tgtcaaagcc tagctttctt gttataatgg ataagatgga
      301 ggcgttatgg cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc gagaaactat
      361 tcaatcaaga tcagcggaaa ccccaagcct acacgactag aagtaaataa attttgtaag
      421 cattgtggca agtacactac acacagagaa acgagatagg agagagcgat gcgttttatt
      481 ggagatattt ttagacttct taaagacaca acgtggccaa ctcgcaagga aagctggaga
      541 gattttcgtt ctatcatgga atacacagct ttctttgtag taattattta catttttgac
      601 cagttgattg tttcaggttt gattcgattt attaacattt tttagaagat tagtggagtt
      661 aattacacta gaaatcttct atttatgaaa ggaaatatca tggatagttt tgataaaggg
      721 tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa tctattacaa
      781 cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc aacacaaaca
      841 gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt tccaggttat
      901 gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa cacaccaaat
      961 gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt attggaacaa
     1021 gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga tttcgatgtt
     1081 gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac tggtaagatt
     1141 acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa tgacacagtt
     1201 gcagaagtaa acctaaacca aatcgcagaa ttataaccct gagagaggct ggagcctctc
     1261 ttttttgtgc agtttaggag ctaaagggaa cagaatggag aaaatggaac aaatgtgttt
     1321 tctaatctgt tagactgtat ctagaaaggg gaaaattatg attaaagaat tgtatgaaga
     1381 agtccaaggg actgtgtata agtgtagaaa tgaatattac cttcatttat gggaattatc
     1441 ggattgggac caagaaggca tgctctgctt acatgaattg attagtagag aagaaggact
     1501 ggtagacgat attccacgtt taaggaaata tttcaaaacc aagtttcgaa atcgaatttt
     1561 agactatatc cgtaagcagg aaagtcagaa gcgtagatac gataaagaac cctatgaaga
     1621 agtgggtgag ctcagtcatc gtataagtga ggggggtctc tggctagatg attattatct
     1681 ctttcatgaa acactaagag attatagaaa caaacaaagt aaagagaaac aagaagaact
     1741 agaacgtgtc ttaagcaatg aacgatttcg agggcgtcaa agagtattaa gagacttacg
     1801 cattgtgttt aaggagttta ctatccgtac ccactaggaa gtcatgcaaa aaaaatgaaa
     1861 aaaattagaa aaagtagttg acaaagtttg aaaagactgt ataatagtaa gagttgaaaa
     1921 taacaactca ggtccgttgg tcaaggggtt aagacaccgc cttttcacgg cggtaacacg
     1981 ggttcgaatc ccgtacggac tatggtatgt tgcggttgga acacttgatg aaaaaaagtt
     2041 taaaaaaact taaaaatctt caaaaaagtg ttgacaagcg aaagcagttg tgatatacta
     2101 atatagttgt cgcttgagag aagcaagtga caaagacctt tgaaaactga acaagacgaa
     2161 ccaatgtgca gggcgctaca acataagttg tagtactgaa caatgaaaaa acaataaatc
     2221 tgtcagtgac agaaatgagt gagaactcaa acttttaatg agagtttgat cctggctcag
     2281 gacgaacgct ggcggcgtgc ctaatacatg caagtagaac gctgaaggag gagcttgctt
     2341 ctctggatga gttgcgaacg ggtgagtaac gcgtaggtaa cctgcctggt agcgggggat
     2401 aactattgga aacgatagct aataccgcat aagagtagat gttgcatgac atttgcttaa
     2461 aaggtgcact tgcatcacta ccagatggac ctgcgttgta ttagctagtt ggtggggtaa
     2521 gggctcacca aggcgacgat acatagccga cctgagaggg tgatcggcca cactgggact
     2581 gagacacggc ccagactcct acgggaggca gcagtaggga atcttcggca atggacggaa
     2641 gtctgaccga gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaag ctctgttgta
     2701 agagaagaac gagtgtgaga gtggaaagtt cacactgtga cggtatctta ccagaaaggg
     2761 acggctaact acgtgccagc agccgcggta atacgtaggt cccgagcgtt gtccggattt
     2821 attgggcgta aagcgagcgc aggcggttag ataagtctga agttaaaggc tgtggcttaa
     2881 ccatagtagg ctttggaaac tgtttaactt gagtgcaaga ggggagagtg gaattccatg
     2941 tgtagcggtg aaatgcgtag atatatggag gaacaccggt ggcgaaagcg gctctctggc
     3001 ttgtaactga cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag
     3061 tccacgctgt aaacgatgag tgctaggtgt tagacccttt ccggggttta gtgccgtagc
     3121 taacgcatta agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg
     3181 acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt
     3241 accaggtctt gacatccctc tgaccgctct agagatagag ttttccttcg ggacagaggt
     3301 gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa
     3361 cgagcgcaac ccctattgtt agttgccatc atttagttgg gcactctagc gagactgccg
     3421 gtaataaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggc
     3481 tacacacgtg ctacaatggc tggtacaacg agtcgcaagc cggtgacggc aagctaatct
     3541 cttaaagcca gtctcagttc ggattgtagg ctgcaactcg cctacatgaa gtcggaatcg
     3601 ctagtaatcg cggatcagca cgccgcggtg aatacgttcc cgggccttgt acacaccgcc
     3661 cgtcacacca cgagagtttg taacacccga agtcggtgag gtaaccgtaa ggagccagcc
     3721 gcctaaggtg ggatagatga ttggggtgaa gtcgtaacaa ggtagccgta tcggaaggtg
     3781 cggctggatc acctcctttc taaggataag gaactgcgca ttggtcttgt ttagtcttga
     3841 gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc aggaggtcag
     3901 cggttcgatc ccgctaggct ccattggtga gagatcacca agtaatgcac attgaaaatt
     3961 gaatatctat atcaaatagt aacaagaaaa taaaccgaaa acgctgtagt attaataaga
     4021 gtttatgact gaaaggtcag aaaataaggt taagttaata agggcgcacg gtggatgcct
     4081 tggcactagg agccgacgaa ggacgtgaca aacgacgata tgccttgggt agctgtaagt
     4141 aagcgatgat ccagggattt ccgaatgggg gaacccaaca ggtagtacct gttacccaca
     4201 tctgttaagg atgtgaggag gaagacgcag tgaactgaaa catctaagta gctgcaggaa
     4261 gagaaagcaa aagcgattgc cttagtagcg gcgagcgaaa cggcagaagg gcaaaccgaa
     4321 gagtttactc ttcggggttg taggactgca atgtggactc aaagattata gaagaatgat
     4381 ttgggaagat cagccaaaga gagtaatagc ctcgtattta aaatagtctt tgtacctagc
     4441 agtatcctga gtacggcggg acacgtgaaa tcccgtcgga atctgggagg accatctccc
     4501 aaccctaaat actccctagt gaccgatagt gaaccagtac cgtgagggaa aggtgaaaag
     4561 caccccggga ggggagtgaa atagaacctg aaaccgtgtg cctacaacaa gttcgagccc
     4621 gttaatgggt gagagcgtgc cttttgtaga atgaaccggc gagttacgtt atgatgcgag
     4681 gttaagttga agagacggag ccgtagggaa accgagtctg aatagggcgc cttagtatca
     4741 tgacgtagac ccgaaaccat gtgacctacc catgagcagg ttgaaggtgc ggtaagacgc
     4801 actggaggac cgaaccaggg cacgttgaaa agtgcttgga tgacttgtgg gtagcggaga
     4861 aattccaaac gaacttggag atagctggtt ctctccgaaa tagctttagg gctagcgtcg
     4921 acattagaga ttcttggagg tagagcactg tttgggtgag gggtccatcc cggattacca
     4981 atctcagata aactccgaat gccaatgaat tatggtcggc agtcagactg cgagtgctaa
     5041 gatccgtagt cgaaagggaa acagcccaga ccaccagcta aggtcccaaa ataattgtta
     5101 agtggaaaag gatgtggggt tgcacagaca actaggatgt tagcttagaa gcagctattc
     5161 attcaaagag tgcgtaatag ctcactagtc gagtgaccct gcgccgaaaa tgtaccgggg
     5221 ctaaaacaat ttaccgaagc tgtggatacc tttataggta tggtaggaga gcgttctatg
     5281 tgtgatgaag gtataccgtg aggagtgctg gaacgcatag aagtgagaat gccggtatga
     5341 gtagcgaaag acaggtgaga atcctgtcca ccgtaagact aaggtttcca ggggaaggct
     5401 cgtccgccct gggttagtcg ggacctaagg agagaccgaa aggtgtatcc gatggacaac
     5461 aggttgatat tcctgtacta gagtatgtag tgatggaggg acgcagtagg ctaactaaag
     5521 cagacgattg gaagagtctg tctaagcagt gaggtgtgaa ttgagtcaaa tgcttaattc
     5581 tataacattg agctgtgatg gggagcgaag tttagtagcg aagttagtga cgtcacactg
     5641 ccaagaaaag cttctagcgt ttaaacatac tctacccgta ccgcaaaccg acacaggtag
     5701 tcgaggcgag tagcctcagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac
     5761 cccgtaactt cgggagaagg ggtgctgact ttaagtcagc cgcagtgaat aggcccaagc
     5821 aactgtttat caaaaacaca gctctctgct aaatcgtaag atgatgtata gggggtgacg
     5881 cctgcccggt gctggaaggt taagaggagt gcttagcgta agcgaaggta tgaattgaag
     5941 ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt
     6001 aagttccgac ccgcacgaaa ggcgtaatga tttgggcact gtctcaacga gagactcggt
     6061 gaaattttag tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag accccatgga
     6121 gctttactgc agtttgatat tgagtgtctg taccacatgt acaggatagg taggagtcta
     6181 agagatcggg acgccagttt cgaaggagac gctgttggga tactaccctt gtgttatggc
     6241 cactctaacc cagataggtg atccctatcg gagacagtgt ctgacgggca gtttgactgg
     6301 ggcggtcgcc tcctaaaagg taacggaggc gcccaaaggt tccctcagaa tggttggaaa
     6361 tcattcgcag agtgtaaagg tataagggag cttgactgcg agagctacaa ctcgagcagg
     6421 gacgaaagtc gggcttagtg atccggtggt tccgtatgga agggccatcg ctcaacggat
     6481 aaaagctacc ctggggataa caggcttatc tcccccaaga gttcacatcg acggggaggt
     6541 ttggcacctc gatgtcggct cgtcgcatcc tggggctgta gtcggtccca agggttgggc
     6601 tgttcgccca ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac agttcggtcc
     6661 ctatccgtcg cgggcgtagg aaatttgaga ggatctgctc ctagtacgag aggaccagag
     6721 tggacttacc gctggtgtac cagttgtctt gccaaaggca tcgctgggta gctatgtagg
     6781 gaagggataa acgctgaaag catctaagtg tgaaacccac ctcaagatga gatttcccat
     6841 gattatatat cagtaagagc cctgagagat gatcaggtag ataggttaga agtggaagtg
     6901 tggcgacaca tgtagcggac taatactaat agctcgagga cttatccaaa
//
LOCUS       DAVLYR010000094         6933 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLYR010000094 DAVLYR010000000
VERSION     DAVLYR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.094"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(141..1022)
                     /locus_tag="VYO63_001459"
     CDS             complement(141..1022)
                     /locus_tag="VYO63_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1672361.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(1161..2489)
                     /gene="vncS"
                     /locus_tag="VYO63_001460"
     CDS             complement(1161..2489)
                     /gene="vncS"
                     /locus_tag="VYO63_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1672362.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(2486..3142)
                     /gene="vncR"
                     /locus_tag="VYO63_001461"
     CDS             complement(2486..3142)
                     /gene="vncR"
                     /locus_tag="VYO63_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1672363.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(3191..3274)
                     /gene="pep27"
                     /locus_tag="VYO63_001462"
     CDS             complement(3191..3274)
                     /gene="pep27"
                     /locus_tag="VYO63_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1672364.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(3454..4833)
                     /gene="vex3"
                     /locus_tag="VYO63_001463"
     CDS             complement(3454..4833)
                     /gene="vex3"
                     /locus_tag="VYO63_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1672365.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGENIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(4885..5532)
                     /gene="vex2"
                     /locus_tag="VYO63_001464"
     CDS             complement(4885..5532)
                     /gene="vex2"
                     /locus_tag="VYO63_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1672366.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(5545..6822)
                     /locus_tag="VYO63_001465"
     CDS             complement(5545..6822)
                     /locus_tag="VYO63_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672367.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         1926 a         1525 c         1356 g         2126 t
ORIGIN      
        1 atgttgtcct attctagttt caaaacgcta tagaagcagc attgtgctag tctagattca
       61 gtttactata ctaaaacgag tagcttgaaa tcaaaaaacc caccctcaca ggcaggtttt
      121 atctgtatta ttcagctaga ttatgcttta ccttctgaac cgaatacgtc gatacgttct
      181 tcaaccgatg cttggatagc ttttacaccg tcagccaaga atttacgtgg gtcgaagagt
      241 tttttcttgt cgtattctgc ttcgtttgct tcgtagtcac gagcaaattt acgagttgcg
      301 ttagcgaatg cgatttggca ttctgtgtta acgttaactt tggcaacacc aagtttgata
      361 gctgcttgga tttgctcatc aggaatacct gatccaccgt gcaatacgat tgggaatcct
      421 ggaagagctt ctgtcaattt ttgcaagtgg tcaaggtcta gaccttccca gtttactggg
      481 taagggccgt ggatgttacc gataccagct gccaagaagt cgataccagt ttcaaccatt
      541 gctttagcgt cttcgattgg agccaattca cctttaccga tgattccgtc ttcttcacca
      601 ccgatagtac caacttcagc ttctactgag atacctttag cgtgtgcttt ttcaacaact
      661 tctttagcca atttaaggtt ttcttcaact ggaaggtgtg aaccgtcaaa catgattgaa
      721 gtataaccaa cttcgataca ctcaagtgca tcttcgtagt gaccgtggtc aaggtggata
      781 gctactggta cagtgatacc cattgattca acaaggttag cgatcaagtt gcgagcaact
      841 ttgtaaccac ccatgtattt agcagcaccc attgaagttt ggatcaaaac tggagctttt
      901 ttagcttctg ctgcgcgcaa gatagcttga gtccactcaa ggttgtttgt gttaaatcca
      961 ccaactgcat aaccgttgtc acgggctgct tggacaaatt tttctgctga aacgattgcc
     1021 attttatcag gcctcctgta tatttttatg ggtcatccca tttacattgt tcattttatc
     1081 actttttgcc aaaaaaatct agtttttccc gcagtttcga ttgattttct tctaactcca
     1141 tctatgtaaa ccctttctcc ctagtcttgg acgacttttg gaaaatctat aaagaaggtt
     1201 aaacgattct cctccatctc gaaacgataa gctaattttt catgttctaa tagactctta
     1261 accacaaaga gccccatacc agaccccttg accttgcgac tagcattgtc agaaaaagac
     1321 tgggctagtt tttcttgttc ctctgagcta cagctatttt cgataaaaag ttctccttct
     1381 ctttctccaa ttcgaactaa gccacctgga acagagtgct taatggcatt gctgatgaga
     1441 ttagaaagaa tcaacttcat aactgatggg tttagataag cctgctgatg ggtcaaacta
     1501 ttgtctatct ggagctctct ttcctttgct agcaaggcat aatctttgac cagattttgc
     1561 gtcatctgga ggaggtcaat tgtttcccta tcatctcgca attcctgcac agaagagagg
     1621 gaaagtatct gcagaacatg gtgattgagt tcatccacaa tccccaaggc aactcccaga
     1681 tactggtctc tatccttata acgaccgata ttctctctca tattttcgat taggattttc
     1741 aaactagcca gcggtgtttt caattcatga gaagctcctc gtaggaattc gaccttcatc
     1801 ttctccagct ggagaatggc ttcattcttt tcatgcaagt ccgcaataac agtcaagaga
     1861 tgctggtaga ggctattgat ttgttccttg agattaccta tctcatcctt agaatccacg
     1921 cgcaatcgca cttgggaatc caggtccatc atccgacggg tcacccgctt gatttccaaa
     1981 atcggtgcaa caatagtccg agcgtagatg taggccacca agagggaaat cagaaaggag
     2041 gccagcaagg tatagggaag aaactggaga ctgatttgct ccgcttcctt ttgtaaatcc
     2101 atggaagcta gaaactggag aatcatagta ccaccgtctt gcgttttcac ctcgcgctcc
     2161 tcaataaaga gagaggttgt ctggcggtct gtgtccagag gaagactgtc cttgacttct
     2221 aacttgtcct cggtcatctc acctttgacg gtccccttga tatcactagt ctgggaatac
     2281 aagtctaaca cttgctcgat actctgccta tctttccctt ctagggactg ggcaatggct
     2341 gttgcctttt gaccaatggt ttcctgacga tgactcagat aagtcgaagg aaaaagaaaa
     2401 taaatagcta aatgaaggca gataaccaga acactaaata tcgagaaggt atagataaat
     2461 atctttgcaa ataaacctgt tcgtttcatt ttcgctccaa tttataacca acattgcgca
     2521 cagtgaggat acaatccaag tctagctttt tccgcaattc cttgatataa acatcaataa
     2581 cacggtcaaa gggaacctca tctgtcgctt tccagacggc atcgataatc tgagatcgag
     2641 tcaaggcccg gccttcattt ttcactagat agtccagaat ttccaactct ttggcattga
     2701 taggcacttc ttgacctgcg aggcttgcac tgtagctttc aaagtccacc ttggtatcct
     2761 tgtaagaaaa gattcgtcct gtatcgtagt agcgcttgaa aatcgcgtcc accctcactt
     2821 ttaaaaggga gagggagaaa ggtttttcca gatagccatc tgccaaagag gcaaaggcac
     2881 tcatcttgta ttcctcatct tgaaaagctg tcaacatcaa gacaggaacc tgactggttt
     2941 tacgaatctc agctaggact tctaagccgt tgagcttggg catctggata tccagtaaaa
     3001 ccagggccac ctcatagcta gaaaattgct ccagagcttc ctgaccgtcc gctgcctcaa
     3061 tagtttcata gccacaatcc gtcaaataat cactgacccc ctcacggatc atctcttcat
     3121 cttctacaat taaaattttc atactttaac tgctctctat tttttatttt tcttagaata
     3181 aatacctacc ctattttcta ttatagtctc ttgctggcct tttgtctgca agcaactgac
     3241 cactagataa aacgttgtga aattcctttc tcataaattc cataacttta gtatattata
     3301 tttaagcact aaagtacaaa gaaagcaact gaaagcaatg attttcacca ctgctttcgg
     3361 atttattttg aattgttaaa tagccattcc tatccactat tcttgaatag aaacacaaga
     3421 tgcaatcttt attctagact cattttttca aatttattca ccatccagca agagctcttt
     3481 tggttgtttt ctaaggagat tgcttgaagc aagcgccata acgagaacca ctagaaccaa
     3541 ggcaaggaca aaaatgatga taaagtctga tgtctgaatg gaaatgtcta ggctcgacaa
     3601 ggtcttgcta aagccatcta cttctgcacc accaccaagg ttagaggctt gagccgcctt
     3661 actagcctgt ttggcaacac ctgaagtcac attggcaagg acagtgtttc caattgcacg
     3721 ggcagtgtaa ttagctagga agtaagcaga aactagagca gggatagcaa tcaagataga
     3781 ttcggtgatg aattgaccca agatacttgc ctgcttgagg ccgatagaga ggagaattcc
     3841 cacttccttg cgacgggcgt tgatccaaag gctgagcaag agggcaagga ggagaactga
     3901 gaagctcaag ctaccccaga agaggaggtt ggccatcttg tacataccag agatagattg
     3961 ctcaagagct gggtagttag aggagctctt gacgagtgtg tagctcttcc agttgatacc
     4021 actgatgcca ttcaactctt tcataacatc atccaagttc ttgtctgctg ttacaaagaa
     4081 ggttgcgtcc ccataaatgg ctgtgtcttc tgtgtatcca taaagttttg cagcagtgtg
     4141 aatgtctgta atagctgtgt tttcgtaaag ttcttgtgag taggttactg ctgacttatt
     4201 atgaccatca aagagtccct tgattgtcac ttcaactgtt tccttggctc ctttttcatt
     4261 atctgcatcg tagatattag agtccagttt aaccttgtcc cctactttcc agccgtgttt
     4321 ggctgccaag tccttgtgca agaggatttt atccttgtcg tcgttggtta agtgctctcc
     4381 ttcgactagt ttataagaac cagagacaaa cttgtcttct ttagaggagt cattgacacc
     4441 tgtaatcatc aagctacttc caaaacgctt ggcacgatca gcagtgagat tcttcttggt
     4501 ttctggcgtt tcaatcaggt catatccagt caaatctccg atagcgttga tacgtttgac
     4561 ataagactca atggccttgt tttcggtgat ttttttgatg ttttcaccct tgatattccc
     4621 agcaccacga ggcgttcctt ggttgacgcg acgattgatt tgcatggaga agctattggt
     4681 gatattttta aaggtctcct gagaagcctt ggcagtagct cccttgattg acaagccgac
     4741 caaactcaag ctcgccatga ggagaataat caggaagatg acaatcgatt tgaaaaactt
     4801 ccttgtaaca taggcaaatg cgttgtgtaa catagattcc ctttctagat tttgttttaa
     4861 tcattctatt aaaataagct caaattattt actagtattg cgcgtttcag tcagtttctt
     4921 atcctttaat tcaagtgtaa tatctgacgc ttgtgccact tctttactgt gagttacgac
     4981 aatcacacat ttacctgttt tctgggcaag tgatttgagt agttcgacaa tatctccagc
     5041 agttttagga tccagatttc ctgttggctc atcagctaga ataactggag cttctgagac
     5101 caaactgcga gcaatggcaa cacgttgctg ttgaccacct gataactgga gaacattccg
     5161 cttgatctgg ctttcatcca aaccaagctc aagaagtgta ttcttgcttg cctttttgtt
     5221 gaccaatcgg atattttcca gcggagaaag ataatctatc aagttataat tttgaaagac
     5281 cagggaaata tggtgcatgc gatggtaaga atagcccttc ttacgaatat cctctccttg
     5341 aaaaaggata gaaccttcaa caggactatc tagaccagca agtagggaca agagtgtgga
     5401 ttttcctgct cctgactccc caataatact gtaaaatttt ccgggttcaa aattataatt
     5461 gatctgatat aggactgctt cagcagtatt cttataacgg taggtaacat cttgtaattg
     5521 taataaagtc atgatttctc cttcttaact aatagatgat aaaatttctt tcggtgattt
     5581 tctaaataag aataggaaac aaagggctac agataagcaa ctaagcagaa ctagaaaaac
     5641 ataggattct gcaaaagata agatgctagt tgataaactg cttgctttgg ctagtgtatc
     5701 ttgtaagctt gcctgatctc cacttgctag tagagtttgg agtaggtaag ttgtgattgc
     5761 gtttcctgca acaaatgctg gaagcaaagc tccaagagat accaaaacta cctctaaaca
     5821 gaattgtagg aagatcgagc tcttgccttt tccaagtgca agtaaaatcc ccacttcata
     5881 gacccgttct ctcaaccaga gagacaaaac cagaattaag gctccagctc ctgctatcaa
     5941 catcccataa aggaagatgg tcaggaaggt ttggaaagtt gcaactgagt ctttgatttg
     6001 ttcaaaagcc ttgttttcct tttcgacttg gtagccttga ttttccaagg ccaagttttc
     6061 tacctgcttc atgagtccgt ccatttcctt aggattttct acatagaagc gtgctgcact
     6121 gacttgagct tcactattgc ccaaaagggt ttggctactt tcatagtctg taaagacttg
     6181 attttcactg aagtcagaag acaagcctgt gaatttctct tgttttttac cagaaaagat
     6241 gccgataatc tcaaactcta ctgtttgtcc ttttccagat tcagactgac cagcatccaa
     6301 gccaatcttg tcatgaagcg aaagaccgtt cttcttagcc aattcttcgt ggataaggat
     6361 tttcttggaa tccccttttt gaaggtgtcg cccttctttt agattgaaag ccgaactggt
     6421 aaaggttaca tccttggatg aatcctcaag agccgttaag ctaaccaagt tattgtctgc
     6481 agctgataaa tcatcacgct ccacgctctg ctcgccagtc actgcttcct tgtcttttag
     6541 ttttgcgacc gtctcaagtt caggagagac attttccagc cccttaatct tgcttacaga
     6601 tgctaggtct gacaacttga atgtctgacc attctctatc ttcttaatag aaaaagatgt
     6661 attgagtgat ttataaagat tgctttctac tgttttgttg gacttcatca gagtcaaaca
     6721 ggctgaaatt ccggccaata agaccaataa aatcagaaat aaaataaaac ttctcagtcg
     6781 ctttctgctg acataagccc aagatctttg gattggattc atttgtcacc tccatatttg
     6841 taagactatt ataaaaccca aatatgaaat gtttatgaaa tacgaaaaaa aaatatcgag
     6901 taggggataa tctctagccc ctctcacacc acc
//
LOCUS       DAVLYR010000095         6913 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLYR010000095 DAVLYR010000000
VERSION     DAVLYR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.095"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            33..1358
                     /gene="brnQ"
                     /locus_tag="VYO63_001466"
     CDS             33..1358
                     /gene="brnQ"
                     /locus_tag="VYO63_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1672368.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            1562..2599
                     /locus_tag="VYO63_001467"
     CDS             1562..2599
                     /locus_tag="VYO63_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1672369.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            2601..3071
                     /locus_tag="VYO63_001468"
     CDS             2601..3071
                     /locus_tag="VYO63_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1672370.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLA
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            3074..3790
                     /locus_tag="VYO63_001469"
     CDS             3074..3790
                     /locus_tag="VYO63_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1672371.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3830..3967)
                     /locus_tag="VYO63_001470"
     CDS             complement(3830..3967)
                     /locus_tag="VYO63_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672372.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            4099..4524
                     /gene="rplK"
                     /locus_tag="VYO63_001471"
     CDS             4099..4524
                     /gene="rplK"
                     /locus_tag="VYO63_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1672373.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            4732..5421
                     /gene="rplA"
                     /locus_tag="VYO63_001472"
     CDS             4732..5421
                     /gene="rplA"
                     /locus_tag="VYO63_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1672374.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            5694..5852
                     /locus_tag="VYO63_001473"
                     /pseudo
     CDS             5694..5852
                     /locus_tag="VYO63_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(6259..6339)
                     /locus_tag="VYO63_001474"
                     /pseudo
     CDS             complement(6259..6339)
                     /locus_tag="VYO63_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            6491..6709
                     /locus_tag="VYO63_001475"
     CDS             6491..6709
                     /locus_tag="VYO63_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1672375.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT         2062 a         1352 c         1503 g         1996 t
ORIGIN      
        1 gtcagtattt tataaatatg aaggagattt ttatggctaa aaaaggtacc ctaacaggtt
       61 tgctcctgtt tggaatattt tttggtgcgg ggaacttgat ttttccgcct tctctaggtg
      121 ctctatctgg agaacatttt cttcctgcca tcgcaggttt tgtcttttca ggcgttggta
      181 tcgccgtctt gacccttatt attggaacgc taaatcctaa agggtatatc tacgagattt
      241 caacgaagat agcgccttgg tttgcgactc tttacctctc agttctttac ttgtcaatcg
      301 gtccattctt tgctacccca cgtactgcta caacagctta cgaagtaggg attagccccc
      361 ttttgtcgga tgcaaataaa ggacttggct tgattgtatt tacggttctg tattttgcgg
      421 cagcctattt gatttcgctt aatccatcaa aaatcttaga ccgcattgga cgtattttaa
      481 cgccagtctt tgcaattttg attgttatct tggtcgttct gggagctatc aaatatggtg
      541 gaacaagtcc tcaagctgct tcagctgctt atcaagcttc tgcctttggt acaggtttcc
      601 tagaaggtta caataccttg gacgcccttg cctcagtggc ctttagcgta atcgcagttc
      661 aaaccttgaa acaacttgga ttttcaagta agaaagaata catttcaact atttgggttg
      721 ttggtatcgt tgttgccctt gccttcagcg ctctttacat cggtttaggt tttcttggaa
      781 atcatttccc agtaccagct gaagcgatga agggtggaac accaggtgtt tacatcttgt
      841 cacaagccac tcaagaaatc tttggctcaa cagctcaact cttccttgca gctatggtta
      901 ccgtaacctg cttcacaacg actgttggtt tgattgtgtc aacagctgag ttctttaatg
      961 agcgcttccc acaaatcagc tacaaggttt atgcgacagc ctttaccttg attggatttg
     1021 ctattgccaa tttgggtctt gatgcgatta tcaagtactc aattccagta ctggttatct
     1081 tgtacccaat cacgattgct atcgttatga ttgtcattgt caacaaattt gttgctcttt
     1141 caaaaccagg tatgcagttg acaattgctg tggttacagt tattgccatt gcaagcgtac
     1201 taggaagctc gtttaaggtt gagtttcttg caaatcttgt tagcgttctt ccttttgcca
     1261 aggcatctct cccatggttg gtgccagcca ttgttggaat cttgctctca ttggttctac
     1321 caaacaagca agaaagcgat gtttttgaaa tggaataatc actaatactc ttcgaaaatc
     1381 aaattcaaac cgcgtcaacg tcgccttgtc gtactcaagt acagcctgcg gctagtttcc
     1441 tagtttgctc tttgattttc attgagtata aaatcacttt tgtagtcaag tctacaggag
     1501 tgattttctt tttttatccg atgataaatg tgttataata ggtagcaaaa gaggtgaaga
     1561 aatgaatcaa acagtagaat atatcaaaga actgacagcc attgcgtcgc caacaggctt
     1621 tactcgtgag attgcggact atttagttaa gactctagaa ggttttggtt accagccggt
     1681 tcgcacatcc aagggcggtg tcaatgtaac tattaaaggt caaaatgatg agcaacatcg
     1741 ctatgtgact gcccatgtag atacgcttgg tgctattgtc cgtgctgtca aaccagacgg
     1801 ccgtctcaaa atggaccgta tcggtggctt tccttggaac atgattgaag gagaaaactg
     1861 taccattcat gtggctagca caggtgaaaa agtatcagga accatcctca tccaccaaac
     1921 ttcttgccat gtctataagg atgcaggaac tgcagaacgc acgcaagaca atatggaagt
     1981 gcgtttggac gccaaagtaa ctagtgaaaa agaaactcgt gctcttggca ttgaggtcgg
     2041 tgattttatc agttttgacc cacgaactgt cgtgacagag acaggtttta tcaagtctcg
     2101 ccatttggat gacaaggtca gtgcggcgat tttgctcaat ctccttcgca tttataagga
     2161 agagaagatt gaattgcccg taacaactca ttttgctttt tcagtctttg aagaagtggg
     2221 acacggtgca aactctaaca ttcctgctca ggtagtagaa tatctggctg tggatatggg
     2281 agccatggga gatgaccagc aaacagacga atatacagtg tctatctgtg tcaaggatgc
     2341 ttctggacct tatcactatg acttccgtca acatttggtg gctttggcga aagagcaaga
     2401 tattccattt aagctggata tctatccatt ttatggttcg gacgcttcag cggctatgtc
     2461 tgcaggggca gaagtcaaac acgcccttct cggtgctggt atagagtcta gccattccta
     2521 tgagcgtacc catattgact cggtgatcgc aacagaacga atggtcgatg cttatcttaa
     2581 gagcacgttg gtggactaat atgtgcctta tttgtcagag aattgacctc atcaagaagg
     2641 aagaaaatcc ttactttgtc aaagagttgg aaacaggcta tcttgtggtt ggagaccacc
     2701 agtattttga aggctatagt ctctttctag ccaaggagca tgtcagcgaa ttgcaccatt
     2761 tgaaaaagga gacaagactc cgttttctag aagaaatgag tttagtccaa gaggcagttg
     2821 ccaaggcctt tgctgctgag aaaatgaata tcgaactgct aggaaatggc gatgctcatc
     2881 ttcattggca tctgtttcca cgacggacag gtgatatgaa tggtcatggt ctcaagggtc
     2941 gtggaccagt ctggtgggtt ccctttgaag aaatgacagc agaaacctgc caagcaaaac
     3001 cggatgagat taaaagatta gtcaaacgtt tatcgtcaga agtagataaa ctattagaaa
     3061 taaaggagta gaaatgaaga aaagatacct agtcttgaca gctttgctag ccttgagtct
     3121 agcagcttgt tcacaagaaa aaacaaaaaa tgaagatgga gaaactaaga cagaacagac
     3181 agccaaagct gatggaacag tcggtagtaa gtctcaagga gctgcccaga agaaagcaga
     3241 agtggtcaat aaaggtgatt actacagcat tcaagggaaa tacgatgaaa tcatcgtagc
     3301 caacaaacac tatccattgt ctaaagacta taatccaggg gaaaatccaa cagccaaggc
     3361 agagttggtc aaactcatca aagcgatgca agaggcaggt ttccctatta gtgatcatta
     3421 cagtggtttt agaagttatg aaactcagac caagctctat caagattatg tcaaccaaga
     3481 tggaaaggca gcagctgacc gttactctgc ccgtcctggc tatagcgaac accagacagg
     3541 cttggccttt gatgtgattg ggactgatgg tgatttggtg acagaagaaa aagcagccca
     3601 atggctcttg gatcatgcag ctgattatgg ctttgttgtc cgttatctca aaggcaagga
     3661 aaaggaaaca ggctatatgg ctgaagaatg gcacctgcgt tatgtaggaa aagaagctaa
     3721 agaaattgct gcaagtggtc tcagtttgga agaatactat ggctttgaag gcggagacta
     3781 cgtcgattaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct tacctactga
     3841 ctgcgtcggt tctattcaca acctcaaaac agtgttttga gctgacttcg tcagttttat
     3901 ctgcaacctc aaagctgtac tttgagcagc ctgcggctag cttcctagtt tgctctttga
     3961 ttttcattga gtacaaaaaa taaacttttc tcttgcaatt ccagataaat agtgtataat
     4021 ggatgggtat gtgaaaaaca tacttgtggg aggtaaaaat ctctaattac cgccaaaacc
     4081 acaaaggagg atttaaaaat ggctaaaaaa gtcgaaaaac ttgtaaaatt gcaaatccct
     4141 gctggtaaag ctacaccagc tccaccggtt ggacctgctc ttggtcaagc tggtatcaac
     4201 atcatgggat tcacaaaaga gttcaacgct cgtacagctg accaagctgg tatgatcatt
     4261 ccagttgtta tctcagttta cgaagataaa tcatttactt tcgttacgaa aacaccacca
     4321 gctgctgttc ttttgaaaaa agctgcaggt gttgaaaaag gatcaggtac acctaataaa
     4381 actaaagttg ctacagttac tcgtgcacaa gtacaagaaa ttgcagaaac taagatgcca
     4441 gatttgaacg cagcaaacgt agagtctgca atgcgtatga tcgaaggtac tgctcgttct
     4501 atgggattca ctgttgttga ctaatcaata acacagtaga aaatctcaca gcagtctatt
     4561 agttgctttt catactaggc aagtgactga ggcttgtact tgggtacagc aagggagctt
     4621 aaggccgtag gaagagaaaa atagtagact gaaaacccgc aagacttcat catttcgaga
     4681 agtgacgtgg gagatgaaaa tcgattgaac cacttacaag gagaatagaa aatggctaaa
     4741 aaaagcaaac aacttcgtgc tgctcttgag aaaatcgaca gcacaaaagc atacagtgta
     4801 gaagaagctg tagcacttgc aaaagaaact aactttgcaa aatttgatgc aactgtagaa
     4861 gttgcttaca acttgaacat cgacgttaaa aaagctgacc aacaaatccg tggagcaatg
     4921 gtattgccaa acggtactgg taaaacttca cgtgttcttg ttttcgcacg tggtgcaaaa
     4981 gctgaagaag caaaagctgc tggtgcagac tttgttggtg aagatgacct tgttgctaaa
     5041 atcaacgacg gttggttgga cttcgacgta gttatcgcta cacctgatat gatggctctt
     5101 gttggacgtc ttggacgtgt ccttggacca cgtaacttga tgccaaaccc taaaactggt
     5161 actgtaacaa tggatgttgc taaagcagtt gaagagtcta aaggtggtaa aatcacttac
     5221 cgtgctgacc gtgcaggtaa cgttcaagca atcatcggta aagtatcatt tgaagctgaa
     5281 aaattggttg aaaactttaa agctttcaac gaaacaatcc aaaaagcaaa accagctaca
     5341 gctaaaggaa cttacgtaac aaacttgact atcacaacta ctcaaggtgt tggtatcaaa
     5401 gttgacgtaa actcacttta atcagtagtt aaagtaatgt aaaaaagttg aagacactat
     5461 gtctcaactt tttttgatgt acgacgggca tgtcgtatat ctgaggtgta agtcctcggt
     5521 gggcacctac taccggtgaa cccaatagcg attcccaagt ctgactatcg tgaggtagcg
     5581 gattaaaacg gtctggggat agaccgtttt aagtctgacg ctggaaataa gaatcgtcag
     5641 aggaagggat agcgaaatcg tggctctacg aacaggaacg tgataataag gcctatatag
     5701 cggataaggt atctggctgg ggcgaccact atcaattagt agctcagaag tcggtactta
     5761 aacgtgctat atcaaaacca gtccttgaaa aacgtggact ggtttcgtgt ttggattatt
     5821 accttgaacg acatgcgtta aaagttagtt gaaccgccgt atgccgaacg gcacgtacgg
     5881 tggtgtgaga ggggctagag attatcccct actcgatttc gaaatttagt ggaatgaatc
     5941 tggaatagtt catcgagctt tctaatactc ttcgaaaatc tcttcaaacc acgtcaacgt
     6001 cgccttgccg tgcgtatggt tactgactac gtcagttcta tccacaacct caaaacagtg
     6061 ttttgagctg actacgtcag ttctatccac aacctcaaaa cagtgttttg agctgactac
     6121 gtcagttcta tccacaacct caaaacagtg ttttgatctg acttcgtcag ttctatccac
     6181 aacctcaaaa cagtgttttg atctgactac gtcagttcta tccacaacct caaaacagtg
     6241 ttttgatctg actacgtcag ttctatccac aacctcaaaa cagtgttttg agcaacctac
     6301 ggctagtttc ctagtttgct ctttggtttt cattgagtat aacacattgt tagaagttgg
     6361 tttaaattcc ctaatcaatt tgttcacatt taccttcgat atattatatc ccatagttaa
     6421 ggttggtcat gcagatgatt atagtcatgg agccgtaaaa cttagtgttt ctttagttga
     6481 caaagatgcc atgaaaaaaa tatctgtaac tgtaatagga tattttgaaa taaatataga
     6541 tgaaaatatc accgatattc tatacgtaaa tggtactgct attctttatc attatttacg
     6601 ttctattgtt tcaatagttt cggcaattga tagcagtgaa gcaatgttgc tacctaccat
     6661 taatgtttta gagttactag ataaatctca accttttgaa gaagaataat ttattagctc
     6721 actaaattga gggtaaggaa aagtaaaatc agtaagaaaa atgtcttgca ttatacagca
     6781 accttttggg aatgagtaga tggattgaat aaaattttat taagagtgga tgatttatct
     6841 gtagattatt attggacagt tagtcttgaa gtagtctaag aattaggtta taatcagtag
     6901 aatccttgct aat
//
LOCUS       DAVLYR010000096         6877 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLYR010000096 DAVLYR010000000
VERSION     DAVLYR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.096"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(78..827)
                     /locus_tag="VYO63_001476"
     CDS             complement(78..827)
                     /locus_tag="VYO63_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1672376.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            960..3296
                     /locus_tag="VYO63_001477"
     CDS             960..3296
                     /locus_tag="VYO63_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1672377.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFGVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            complement(3733..6855)
                     /locus_tag="VYO63_001478"
     CDS             complement(3733..6855)
                     /locus_tag="VYO63_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEV1672378.1"
                     /translation="MTVPDRLPARMRMDGRSIFQQIKNYFDTENKEYFKHPNTYDGIS
                     MHLEPNILTSMEHFDLTGFHCECKDFQNQGVCKHWVAMDLYFRSLPQAIQERIGKASH
                     KPSFASQLIPTLPSEELQDELVEEKATAPSLALHGQVEIRNHSLAWTLKLQVEQAPRA
                     YVIKDIAHFIFLIFQKEDYFVSQKIGTIRLSLNQFNQASQNLLLYIKKYFIDRNEHSY
                     FNFSYGINPRDYGRYLETPVSYLNDLVPLFQALDVFQYVTSKAEYPLIFLDDSPFIPE
                     EEIFKVVKSNNHYEIINTAYFGFIIQEKLWIRHNHFHIIKEEHRYFLDKLATWIYHYQ
                     ENSPLIFSKENKAELMQVCNIISNYVPISIPDELQIHDFIPTFAFSKTRNEIALNMVW
                     SFGEKQVHSKQDLLTLPYTYQASKARKIYHQLLSAGFKEEFHSLSKIKIVDFFLKELP
                     RFRTLGQVQLDESLEKLLVEDPAVIDIFDDESFLSVQFDFSMISEDEVEKAIQALWNQ
                     ESHYQTKQGKVLVFDDESLKVAQSLQDLRAKFSDGKIKMHKSRAFSLSETFKDNEHVN
                     FSRDFKKMAYDLTHPEEFDIKPYEVKAKLRSYQKEGVKWLSMLDHYHFGGILADDMGL
                     GKTLQTITLLEANLKPDQKALILAPASLLYNWKEEFRKFVPHKQVEVAYGSKTERIKQ
                     IEKSATITITSYPSFRSDLEHYQKQSYDYLILDEAQMIKNSQTKTAQALREFDVKTCY
                     ALSGTPIENRLEEIWSIFQIVLPGLLPSKKEFSKLSPQLVAKLIQPFVLRRKKDEVLT
                     ELPELSEHLYSNELSSSQKTLYLAQLRRMQEMVSGASAYEIKRHKIEILAGLTRLRQI
                     CNTPALFLEDYKGDSGKMDSLFELLDTIREKGSRPLIFSQFTSMLDLIEQELEKKEMS
                     HFKITGQTPSDKRQEMVNLFNQGEKDCFLISLKAGGTGLNLTGADTVILCDLWWNPAV
                     EMQAIGRSHRLGQTKQVDVYRLITLGTIEEKIQELQESKKELFNTVLEGQESRSNLSV
                     DDIKEILGVE"
BASE COUNT         1851 a         1311 c         1577 g         2138 t
ORIGIN      
        1 aagtagaggt gtactattct agttccaatc tactatataa atgaaaacct tagctgtttg
       61 gccaaggttc atttcttcta tgcaagttct tctcttttct tatctgggtt ccagatgaag
      121 cttgcgataa agctaaagat gatggttagg atagcgagga taatagcaag gatgagggca
      181 gggatgcgga taaaccacca gagtggattt ggttttttat cattgtgcca ttgtttcgtt
      241 tcttcgtcca gacgttggaa ttctgtttga atacgattgg cgactgtttc aatgccttca
      301 tcattcattt tcttgatatc tgagatatcg attggatttc caaagttcat atcgacacgt
      361 tcacggctaa tcaagccctt caaagtcatg ggaccggtgt aggtaaccgg catgatacgg
      421 accttggcca ttttggcaat cagtgctgcg ccccccttga catcgtttga gtggcggcta
      481 ccacttggaa acatgatgag agagcggtca ctttttttga gaacgttgat aggatatttg
      541 atggctgagg cgctgggatt ttcacggtcg atgggaaagg cgccacacat acgaatccac
      601 caaccaaaga tacggttggt aaagagttct ttttttgcca taaagatgaa ctgttttggc
      661 ttggtcgcaa aggccatata aacaggatcc caccaggtac ggtgaggcgc aactaaaata
      721 taattttcat cttgattagg aattttatca gtattatgat agtgagcatt gccattgatg
      781 gaccataaga gcaatacaac taatccacgc aaataagtat aaaacatgcg atctccttcg
      841 attgttttct tgttattatt ataccttatc aaaggagggc tggcaaactt ttcccttgac
      901 tagatgcata tttgggatga aattagaatt ctgttaaaaa aaatgatata atagaattta
      961 tggataaaaa taagattatg ggattaaccc aaagagaagt caaggaaaga caggctgagg
     1021 gtttggtcaa tgactttacc gcatcagcca gtaccagcac ttggcaaatc gttaaacgaa
     1081 atgtctttac cctttttaac gctttgaact ttgccattgc tttggccctt gcctttgtgc
     1141 aggcttggag caatctggtc ttctttgctg ttatctgctt taacgctttt tctgggattg
     1201 tgaccgagct acgagccaaa cacatggtgg acaagctcaa tctcatgacc aaggaaaagg
     1261 tcaaaaccat ccgtgatggt caggaagttg ctcttaatcc tgaagaatta gtgctaggag
     1321 atgtcattcg tttgtctgca ggagagcaga ttcctagtga tgccttggtt ttggaaggct
     1381 ttgcggaagt caatgaagcc atgttaacgg gagaaagtga tttggtgcaa aaggaagttg
     1441 acggcttact tttgtcagga agtttcctag ccagtgggtc agttttatct caagttcacc
     1501 atgtcggtgc agacaactat gctgccaaac tcatgcttga ggctaagacc gttaaaccca
     1561 tcaactcccg tatcatgaaa tcgctggaca agttggctgg ttttactggg aagattatca
     1621 ttccctttgg tctggctctc ttgctggaag ccttgctttt aaaaggcctg cctctcaagt
     1681 catctgttgt aaactcgtcg acagctcttt tgggaatgtt gcctaaggga attgcccttt
     1741 tgaccattac ttcgctcttg actgcagtga ttaagctagg tttgaaaaag gtcttggtgc
     1801 aggagatgta ctctgttgag accttggcgc gcgtggatat gctctgtctg gacaagacgg
     1861 gcaccatcac ccaaggaaag atgcaggtgg aggctgttct tccactgacg gaaacttacg
     1921 gtgaagaggc tattgccagc atcttgacta gctacatggc ccatagtgag gataagaatc
     1981 caactgccca agccattcgc cagcgttttg tgggagatgt tgcttatcct atgatttcca
     2041 atcttccctt ctcgagcgac cgcaagtggg gggctatgga gttagaaggc ttggggacag
     2101 ttttcttagg ggcacctgag atgttgctgg attctgaagt cccagaagcc agggaggcct
     2161 tggagagagg atcacgtgtc ttggtcttag ctctcagtca ggagaaatta gaccatcaca
     2221 aaccacagaa accatctgat attcaggctc tagccttgct ggaaatcttg gaccccattc
     2281 gagagggagc agcagagacg ctggactatc tccgttctca ggaggtggga ctcaagatta
     2341 tctctggtga caatccagtt acggtgtcca gcattgccca gaaggctggt tttgcggact
     2401 atcacagcta tgtagattgc tcaaaaatca ccgatgagga attgatggcc atggcggagg
     2461 agacagctat tttcggacgt gtttcccctc atcaaaagaa actcatcatc caaacgttga
     2521 aaaaagcggg tcatacaacg gctatgacag gggacggggt taatgatatc ttggcccttc
     2581 gtgaggcgga ttgttctatc gtgatggcgg agggggatcc agcaacccgt cagattgcca
     2641 atctggttct cttgaactca gactttaatg atgttcctga gattctcttc gagggtcgtc
     2701 gcgtggtcaa taacattgcc cacatcgccc cgattttctt gataaagacc atctattcct
     2761 tcctgttagc agtcatctgt attgccagtg ctttactagg tcggtcagag tggattttga
     2821 tttttccctt cattccgatc cagattacca tgattgacca gtttgtggaa ggtttcccac
     2881 cattcgttct gacttttgag cgaaatatca aacctgtcga gccaaacttc ctcagaagat
     2941 ccatgcttcg tgccctacca agtgccctca tggtcgtgtt tggcgttctt tttgtgaaaa
     3001 tctttggaac gagtcaaggt tggtctgagt tagaaatctc aactctcctc tattatctct
     3061 tggggtcaat tggtttctta tccgtattta gagcctgcat gccattcact ctatggcgag
     3121 tcctattgat tgtctggtca gtaggaggct tcctagccac agctctcttc ccaagaattc
     3181 aaaaactgct tgaaattgca accttaacag gacaaacatt acctgtttat ggtgtcatga
     3241 tgttggtctt tacagtgatt ttcatcctga ctagtcgcta tcaagctaga aaataaagaa
     3301 agactgcaat ctgtggattg cagtttttta tacgatggat ctatcgtaga tctgatgtgc
     3361 aaggcctacg tgtctattat ctatcggtga acccaagagt gattctcaag cctgcttgga
     3421 ttgaggtaat agattcaaat atctgtagtt agactatttg aagtctgatg caagaaataa
     3481 ttctcttcga aaatatcttc aaaccaggtc cgttctatcc acaatctcaa agcggtactt
     3541 tgagcaactt gtggtcagct ccctagtttg atagtataaa aactgtcaga taaaggggtg
     3601 gcgaaattgt atctaaacga acagaaacgt gatggtaagg cgtatagagc tcataggatg
     3661 gcaaggaaag tctagaacct aaaaaagaac tagataaatc cactaagtgt ggttactcta
     3721 gttctttttc gtttattcaa cacccaagat ttctttaata tcatcaacgc tcagattgct
     3781 tctgctttct tgtccttcaa ggacggtgtt gaagagttcc tttttacttt cttggagttc
     3841 ttgtattttt tcctctatgg taccaagtgt gataaggcga tagacatcga cttgtttggt
     3901 ctggccaagg cggtggcttc ggccgatggc ttgcatctca actgctggat tccaccagag
     3961 gtcacagagg atgacggtat cagctcctgt tagattcagt cctgttccac ctgcttttag
     4021 ggaaatgagg aagcagtctt tttccccttg attgaaaaga ttgaccattt cttgacgttt
     4081 atctgaaggt gtttgtcctg ttattttaaa gtgagacatt tcttttttct ctagttcttg
     4141 ctcaatgaga tcaagcatag aggtgaattg tgaaaagata agtggtcgtg accctttttc
     4201 acgaatggtg tctaggagct cgaataagct gtccattttt ccactatccc ctttgtagtc
     4261 ttctaaaaag agagcagggg tattacagat ttgtctaagt cgggtcagtc ctgctaagat
     4321 ttcaatttta tgacgtttaa tctcataggc gctggctcct gaaaccattt cttgcatgcg
     4381 tcgaagttga gctagataga gcgttttttg cgaactgctg agttcattgc tgtagaggtg
     4441 ctcgctcagt tcaggcaatt ctgtcagaac ttcgtctttt ttcctacgca gaacaaaagg
     4501 ttgaatcagt ttagcaacga gttgaggtga caattttgaa aattcctttt tacttggtag
     4561 gagacctggt aatactatct ggaaaataga ccaaatctct tcgagacggt tttcaatagg
     4621 agttccagat agggcatagc aggttttgac atcaaattct cttaaagctt gtgctgtttt
     4681 ggtctgacta ttttttatca tctgtgcttc atctaggatg aggtagtcgt aagactgctt
     4741 ttgataatgt tcaaggtcag atcgaaaaga tggatagctt gtaatagtga tagtagcaga
     4801 tttttcaatt tgtttgatac gctcagtttt gctaccatag gctacttcaa cctgtttgtg
     4861 aggcacaaat tttcggaatt cttccttcca attataaaga aggctggctg gagctaggat
     4921 aagagctttc tggtctggtt taaggttggc ttccagaaga gtgatggttt gcaaggtttt
     4981 ccctagaccc atgtcgtctg ccaggattcc cccaaaatga taatgatcca gcatactcag
     5041 ccacttgact ccttcttttt gataggagcg gagtttggcc ttgacttcat aaggcttgat
     5101 atcaaattct tcagggtggg tcaggtcata agccatcttt ttgaaatcac gagaaaaatt
     5161 gacatgttca ttgtctttga aggtttcaga aaggctaaag gcacgagatt tgtgcatctt
     5221 gatttttccg tctgaaaatt tagcgcgaag atcttggaga ctttgagcaa ccttgagact
     5281 ttcatcgtca aagactagaa cctttccttg cttggtttgg tagtggcttt cttgattcca
     5341 taaagcttgg atagcttttt caacctcatc ttctgaaatc atagaaaaat caaactgaac
     5401 tgaaaggaag ctttcgtcgt caaaaatgtc aatgacagca ggatcttcta ctaagagttt
     5461 ttcaagcgat tcatctagtt gaacttgacc cagagtacga aaacggggga gttcttttag
     5521 gaagaaatca acgattttga ttttggaaag actatgaaat tcctctttga aaccagcaga
     5581 aagcagttga tgatagattt ttctagcttt gctagcttgg taagtatagg ggagggttag
     5641 gagatcttgt ttagaatgga cttgtttttc tccaaaggac cacaccatat taagggcaat
     5701 ttcatttcta gtcttggaga aggcaaaggt ggggataaag tcatggattt gtaattcatc
     5761 tgggatactg ataggaacat agtttgagat gatattacag acttgcatga gttcagcctt
     5821 attttctttt gaaaagataa gaggagaatt ttcttgataa tgatagatcc aagtagctaa
     5881 tttatccaaa aagtaacggt gttcttcttt gatgatgtgg aagtgattgt gtcgaatcca
     5941 aagtttttct tgaataatga aaccaaaata agctgtattg atgatttcat aatggttatt
     6001 agatttgaca actttaaaaa tttcctcttc cgggataaag ggactatcat ctaagaatat
     6061 aagagggtat tcggcttttg aggttacata ttggaagaca tccaaagctt gaaaaagtgg
     6121 gacgaggtcg tttaaatagc taacaggagt ctctaaatag cgtccgtaat ctctagggtt
     6181 aatgccataa ctgaagttga aataagaatg ttcatttcta tcgataaaat attttttaat
     6241 atagaggagt aggttttggg atgcttgatt aaactgattc aaggataatc tgattgtccc
     6301 gattttctgt gaaacaaagt aatcttcctt ttggaaaatg agaaaaatga agtgagctat
     6361 atccttgata acataggcac gaggagcttg ttctacctgt aattttaagg tccaggctag
     6421 gctatgattt cggatttcga cttgtccatg aagggcaagt gaaggcgccg tagctttttc
     6481 ttctaccagt tcatcttgga gctcttcaga tggcaaggta ggaatcagtt ggctggcaaa
     6541 acttggttta tgactcgctt taccaatgcg ttcttgaata gcttggggga gactacgaaa
     6601 ataaagatcc atggcaaccc agtgtttaca gactccctga ttttggaaat ccttacattc
     6661 gcaatgaaag ccagtcaaat cgaagtgctc catactggtc aggatgttgg gctccagatg
     6721 catggaaata ccatcatagg tattggggtg cttaaaatat tccttatttt cagtatcaaa
     6781 atagtttttt atctgttgga aaatagagcg gccatccatt ctcatacgag caggtagtct
     6841 atcgggaacc gtcattgaag taacctctgt tctaatc
//
LOCUS       DAVLYR010000097         6769 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLYR010000097 DAVLYR010000000
VERSION     DAVLYR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.097"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..111
                     /locus_tag="VYO63_001479"
     CDS             <1..111
                     /locus_tag="VYO63_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
                     /protein_id="HEV1672379.1"
                     /translation="ISRSKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(162..368)
                     /locus_tag="VYO63_001480"
     CDS             complement(162..368)
                     /locus_tag="VYO63_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1672380.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDILEAVKNCPTRALIGNQEA"
     gene            420..896
                     /locus_tag="VYO63_001481"
     CDS             420..896
                     /locus_tag="VYO63_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1672381.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKGFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMVTGSWFANPGDVIKIK"
     gene            905..1576
                     /locus_tag="VYO63_001482"
     CDS             905..1576
                     /locus_tag="VYO63_001482"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1672382.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            1707..2045
                     /locus_tag="VYO63_001483"
     CDS             1707..2045
                     /locus_tag="VYO63_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1672383.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            2048..2677
                     /locus_tag="VYO63_001484"
     CDS             2048..2677
                     /locus_tag="VYO63_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1672384.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            2917..4068
                     /locus_tag="VYO63_001485"
     CDS             2917..4068
                     /locus_tag="VYO63_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1672385.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            4381..5130
                     /gene="truA"
                     /locus_tag="VYO63_001486"
     CDS             4381..5130
                     /gene="truA"
                     /locus_tag="VYO63_001486"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1672386.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            5120..5893
                     /locus_tag="VYO63_001487"
     CDS             5120..5893
                     /locus_tag="VYO63_001487"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1672387.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            5871..6332
                     /locus_tag="VYO63_001488"
     CDS             5871..6332
                     /locus_tag="VYO63_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1672388.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     AIIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            6559..>6769
                     /locus_tag="VYO63_001489"
     CDS             6559..>6769
                     /locus_tag="VYO63_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672389.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEMINKV"
BASE COUNT         1947 a         1231 c         1492 g         2099 t
ORIGIN      
        1 ataagtcgca gtaagattta tttagagact aaaaaaagac cactttatct tatcaaagaa
       61 aaaagtgacc ttcctgattt tacagaaaaa aataaagtga aaagactata attttacatg
      121 gaaatgtgct aaactagagg gagtggatta tcgccattga tttaggcttc ctggtttcca
      181 atcagggcgc gagttgggca atttttaaca gcctctaaga tatcctgact aggagaaatt
      241 tctttttcca gttggtcagg gtcatcgtaa aaacgcacga ttccattatc gtggtaatca
      301 aataaatcag aataagtttg gcaaagccca caggcgatac atcgttcagg tataagtgtg
      361 attttcatgt tcatatttat attgtaataa gaaaagtaaa aaaaaacaag gagtaaggta
      421 tggcaaaaga accgtggcaa gaagatatct atgatcaaga agaatcaaga gcagagcgtc
      481 ggcatcgaaa ccacggaggg gctgatagga tggctaatcg tattttgacg atcctagcta
      541 gtattttctt tgtaattgtg gtggtgatgg tcatcgttct catctatcta tcatcggggg
      601 ggagtaatcg cacagcagcc ttaaaaggct ttcatgattc tgatgcaagt gtagtacaaa
      661 tctcatcttc aagcagttct cagcctgagc agagttcaga gccagaatct acttctagta
      721 gttcagaaga agctgctaat cctgaaggaa cgattaaagt tctcgcagga gaaggggaag
      781 cagctattgc cgctcgtgca ggaatctcca ttgctcagtt agaggccttg aatcctgggc
      841 acatggttac aggatcttgg tttgctaatc caggtgatgt tataaaaata aaataggagc
      901 cgatatgaaa acaattcaaa ttgctattga tggtcctgct tccagcggta agagtacggt
      961 cgcaaagatt attgctaagg attttggatt cacctacctt gatacaggag ctatgtatcg
     1021 tgcagcgacc tatatggctc ttaagaacca attaggagtt gaagaagtcg aagcccttct
     1081 agccttgttg gaccagcatc caatcagctt tggacgttca gaaactggag accagcttgt
     1141 ttttgtagga gatgtggata ttacccatcc tatccgtgaa aatgaagtga ccaatcatgt
     1201 ttctgctatt gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc aacaagaaat
     1261 tgcccagcaa ggcgggattg tcatggatgg tcgcgatatt ggaactgttg tattgccaca
     1321 agcagaattg aaaattttcc tagtagcttc tgttgatgag agagcagagc gtcgttacaa
     1381 ggaaaatatt gccaagggaa ttgaaacaga ccttgaaacc ctaaaaaagg aaattgctgc
     1441 gcgtgactac aaggatagtc atcgtgagac ttctcctctc aaacaagcag aggatgctgt
     1501 ctaccttgat acaactggtt tgaacattca agaagtagtt gaaaaaatca aagcagaagc
     1561 tgaaaaaaga atgtaaatgt agaaaagccg atagattgat atcggttttt ttctaaattt
     1621 gtcaaaatgc tgattttagg gtataatagt tgctgttcga gaaattttga ttttcaaaga
     1681 atagtgaatg atgataagga gaaaccatga acaatttacc aaattgtcca aaatgtaact
     1741 cagagtatgt ctacgaagac ggtgccctac tggtttgccc agagtgtgct catgagtgga
     1801 atcctgctga agttgcagaa gtagaagaag gtcttgtcgc tatcgatgcc aacggaaata
     1861 aattgactga tggtgataca gtaactctca tcaaggactt gaaagtaaaa ggtgcgccaa
     1921 aagatttgaa acaagggacg cgcgtgaaaa atatccgcat cgtagaaggc gaccacaata
     1981 tcgactgtaa gattgatggt tttggtgcca tgaaacttaa atcagagttt gtgaggaaga
     2041 tttaagcatg tcaaaacact ataaacttgt attttatagc cgtatcttct tgtttctagc
     2101 ggcttttacg ggagtttatc ttgaaattac taagcatggt ggttttggga tgcttctcta
     2161 ttacacggtt ctgtccaacc tcttggtaac tatttttacc ctttatcttc taaaggttat
     2221 gagccgtgta ggtgaaaatt ggcaaaggcc aagtctcttg cgcttaaaag gtggggtcac
     2281 catgagtatc atgattacct gtgtgattta ccatttcctc ttggcgccca ttgcgactaa
     2341 tttctatacc ctagaaaatt tcctttgcca ctatatcgtt cccatctggt ttttagcgga
     2401 taccctcttt tttgacaaac agggtcaata caagatttgg gatccagcag tgtggaccat
     2461 tttacccttt ctgtatatga tgtttgctct ttttaatggc ttggttctaa aactcaatat
     2521 tccaaatgct aaggataatc ctttccctta cttctttttg aatgtgaaca agggttggaa
     2581 tgttgtgttt aagtggtgtc tgattatctt tgttgcctat atggtagcag gatttatttt
     2641 ctactttatc aagcaaatca agagaaagtc atcctaatac tcttcgaaaa tctcttcaaa
     2701 ccacgtcagc ttccatctgc aacctcaaaa cactgttttg aacaacctgc ggctagcttc
     2761 ctagtttgct ctttgatttt cattgagtat aagatacaag ggattcattt tcgaatttaa
     2821 gaaggagtct acaaaccaat gaggcttctt cttttcttgc ttatttgata actatcaaaa
     2881 aagtagaaga ttaagagaaa tatagaaaga gatttcatga aacaattttt agaacgggcc
     2941 agcattttgg ctctctccct cgttttgatt acctcctttt ccatttcgag tgccctacca
     3001 gccatgtttg actattatca gggttattct aaggaacaaa ttgagctctt ggtgagcttg
     3061 ccttcctttg gaatcatgat gatgttactg ctaaatggtt tcttagaaaa aatatttcct
     3121 gagcgcttac agattagttt gggcttgctg attttatcat tgagcggtac agctcccttc
     3181 tggtaccaag cctatccctt tgtctttgga acacggcttc tctttggttt gggtcttggg
     3241 atgatcaatg ccaaggccat ttctattatc agtgaacgct accaaggaaa aaggcgaatt
     3301 cagatgttag ggctacgcgc ttctgcagag gtcgttggag cttctctcat taccttggcc
     3361 gtcggtcagt tgttggcctt tggttggaca gctatctttc tagcctatag tgctggattt
     3421 ttggtgctgc ccctttatct gctctttgtc ccttatggaa aatcaaagaa agaagtcaag
     3481 aaaagagcga aggaagcaag tcgtttaact cgagaaatga aaggcttgat ttttacctta
     3541 gctatcgaag cggcagttgt agtttgtacc aatacagcta ttaccatccg tattccaagt
     3601 ttgatggtgg aaagaggatt gggggatgcc cagttatcta gttttgttct tagtatcatg
     3661 cagttgatcg ggattgtggc tggggtgagt ttttctttct tgatttctat ctttaaagag
     3721 aaactgctcc tctggtctgg tattaccttt ggcttggggc aaatcgtgat tgccttgtct
     3781 tcatccttgt gggtggtagt agcaggaagt gttctggctg gatttgccta tagtgtagtc
     3841 ttgacgacgg tctttcaact tgtctctgaa cgaattccag ctaaactcct caatcaagca
     3901 acttcatttg ctgtattagg ctgtagtttc ggagccttta cgaccccatt tgttctaggt
     3961 gcaattggct tactaactca caatgggatg ttggtcttta gtatcttagg aggttggttg
     4021 attgtaatct ctatctttgt catgtaccta cttcagaaga gagcttagga ttgattccta
     4081 agtttttctt ttgatactca atgaaaatca aagagcatag ttgattgagt ttggaatagt
     4141 acgctgtagc tgctaaaata tttctagaaa ttaatttgac ttttctaata gagttattca
     4201 tatcttattt caatctacta tagaaagcta gccgcaggtt gctcaaaata gtgttttgag
     4261 gttgcagatg gaaactgacg tggtttgaag agattttcga agagtatgag atttttatac
     4321 ccatacaata tttgttttag gtccttgttt acagactaat ttcttgataa aatagaggtt
     4381 atgacgagat ataaagcaac tatttcctat gatggttatg cctttgctgg ctttcagcgc
     4441 cagtctcatg cgcgtagcgt tcaggaagaa attgaaaaaa ccttgaccag attaaataaa
     4501 gggcaaacca ttactgttca cggtgctggt aggacagata gtggggttca tgccctggga
     4561 caggtcattc attttgacct gccttatcag atggatgagg agaaactccg ttttgccttg
     4621 gatacccagt ctcctgaaga tattgatgtg atttcgattg agcttgtggc agatgatttt
     4681 cattgccgtt atgccaaaca tagcaagacc tatgagttta ttgtggatag aggacgtccc
     4741 aaaaatccta tgcgccgtca ctatgccacc cactttccct acccactcga tgtggaacga
     4801 atgcagattg caatcaaaaa gctagaggga acccatgatt ttaccggttt tacagcctct
     4861 gggactagtg tagaggataa ggttcgcacc atcacagaag ctagtttaat agtcgatgag
     4921 acaggacaat ttttgacctt taccttttca ggaaatggtt tcttgtataa acagattcgc
     4981 aatatggtgg ggactcttct caaaatcggt aacaaccgca tgccagtaga gcagattgat
     5041 ctcatcttgg agaagaagga caggcaactt gcaggtccca ctgcagcacc aaatggtttg
     5101 tatttaaagg agattcgtta tgaagaataa tcgtatttta gcactttctg gaaatgatat
     5161 ttttagtggt ggtggactgt cagctgattt ggctacctat accttgaacg gcttgcatgg
     5221 gtttgtagca gtgacttgtt tgacagcctt gacagaaaaa ggatttgaag tctttccaac
     5281 tgatgatacc atttttcaac atgaattaga tagcttgcgt gatgtggaat ttgggggaat
     5341 taagattggt cttctcccta ctgtcagtgt ggctgagaag gccttggact ttatcaaaca
     5401 acgcccagga gtacctgtgg tgttggatcc tgtcttggtc tgcaaggaaa cgcatgatgt
     5461 agctgtcagt gagctctgcc aagagttgat tcgctttttc ccttatgtca gtgtgattac
     5521 gcctaatctc ccagaagcag aattattatc cggtcaggaa attaaaacct tggaagacat
     5581 gaaaactgca gcgcagaaat tgcatgattt aggagctcca gcagtcatta tcaagggagg
     5641 caatcgtctt agtcaggaca aggcggtgga tgtcttttat gatggacaga cctttactat
     5701 cctagaaaat ccagttatcc aaggccaaaa tgctggtgca ggttgtacct ttgcctctag
     5761 cattgccagt cacttgatta aaggtgataa acttttgcca gcagtagaaa gctctaaggc
     5821 tttcgtttat cgtgctattg cacaagcaga tcagtatgga gtaagacaat atgaagcaaa
     5881 caaaaacaac taaaatcgcc cttgtatccc tattaaccgc cctttctgtg gttctaggtt
     5941 atttcttaaa aatcccaaca cctacaggaa ttctaactct tttagatgct ggtgtcttct
     6001 ttgcggcctt ttactttggt agtcgtgaag gagcggtagt cggaggacta gcaagtttct
     6061 tgattgacct cttatcaggc taccctcagt ggatgttctt tagcttggtc aaccatggct
     6121 tgcagggatt tttcgcagga tttaaaggaa aaagtcagtg gttaggcctt attttagcaa
     6181 ttattgccat ggtaggaggc tacgccttgg gttctacttt gatgaatggc tgggcagcag
     6241 ccctcccaga aattctacca aatttcatgc aaaatatggt agggatgatt gtaggattta
     6301 ttcttagtca aagtatcaag aagattaagt aatactcttc gaaaatctct tcaaaccacg
     6361 tcagcttcca tctgcaacct caaaccactg ttttgagcag cctgaggcta gctttctagt
     6421 ttgcactttg attttcattg agtataaaga ggttgagtaa aaagtatttc aaataagaaa
     6481 aacgcatagt atcaggtgtt gaatgaactg ccccccaaaa gttagacaga aaaaatctaa
     6541 cttttggggt gttttattat gaaactaact tatgatgata aagttcagat ctatgaactt
     6601 agaaaacaag gatatagctt agagaagctt tcaaataaat ttgggataaa caattctaat
     6661 cttaggtata tgattaaatt gattgatcgt tacggaatag agttcgtcaa aaaaggaaaa
     6721 aatcgttact attctcctga tttaaaacaa gaaatgatta ataaagtct
//
LOCUS       DAVLYR010000098         6647 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLYR010000098 DAVLYR010000000
VERSION     DAVLYR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.098"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(73..165)
                     /locus_tag="VYO63_001490"
                     /pseudo
     CDS             complement(73..165)
                     /locus_tag="VYO63_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(201..484)
                     /locus_tag="VYO63_001491"
                     /pseudo
     CDS             complement(201..484)
                     /locus_tag="VYO63_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(474..614)
                     /locus_tag="VYO63_001492"
     CDS             complement(474..614)
                     /locus_tag="VYO63_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1672390.1"
                     /translation="MVSCHSSLRSYIAEPILKQAGFTVQNLDGAYSLYKMVNPEGVEY
                     GN"
     gene            706..1470
                     /locus_tag="VYO63_001493"
     CDS             706..1470
                     /locus_tag="VYO63_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1672391.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            2035..3414
                     /locus_tag="VYO63_001494"
     CDS             2035..3414
                     /locus_tag="VYO63_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1672392.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3428..4093
                     /locus_tag="VYO63_001495"
     CDS             3428..4093
                     /locus_tag="VYO63_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1672393.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4228..4302
                     /locus_tag="VYO63_001496"
                     /pseudo
     CDS             4228..4302
                     /locus_tag="VYO63_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4448..4987
                     /locus_tag="VYO63_001497"
     CDS             4448..4987
                     /locus_tag="VYO63_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1672394.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            5054..5464
                     /locus_tag="VYO63_001498"
     CDS             5054..5464
                     /locus_tag="VYO63_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1672395.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            5580..>6647
                     /gene="pavB"
                     /locus_tag="VYO63_001499"
     CDS             5580..>6647
                     /gene="pavB"
                     /locus_tag="VYO63_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1672396.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKAS"
BASE COUNT         1810 a         1281 c         1522 g         2034 t
ORIGIN      
        1 aaaacagtgt tttgagctga cttcgtcagt tccatccaca accttaaaac agtgttttga
       61 gctgacttcg tcagttccat ccacaacctt aaaacagtgt tttgagcagc ctgcggctaa
      121 cttcctagtt tgcactttaa ttttcattga gtattacttc atcttctcat gaaattgagt
      181 ttttgcccag ctcctcttga ttattctact tgaccgggcc aagcattcat accaccttct
      241 acgttggtaa cggtgaggcc ttgggagctg agaaattggc aggtagatgc agagcggact
      301 cctccgtgac aaatgacata gtattcatgg tcaggtttga gttctttgta gccttgctct
      361 aaggtattta tggaagattt ttgggtcctg cttggaattc atgtgcttca cggacatcga
      421 taagttccag attttcattt tgatattttc tataaaagtc agccatgctg atactagttt
      481 ccatattcta ctccttctgg gttaaccatt ttgtatagtg aataagcgcc gtcaaggttt
      541 tggacggtaa atcctgcttg cttgaggata ggctctgcga tatagctgcg caaactactg
      601 tggcaactga cgatgtaggc ttgtttttct cctcagttca tcagcttcct ccttgacact
      661 cggtcagatt ttgatacaat agtacaaaat tagaggaggt gggctatgat tcagaaacat
      721 gcgattccca ttttagagtt tgatgataat cctcaggcgg ttatcatgcc caatcacgag
      781 gggttggatt tgcagttgcc aaagaagtgt gtctatgctt ttttaggtga ggagattgac
      841 cgctatgcga gggaagtagg ggcgaactgt gttggcgaat tcgtttctgc taccaagacc
      901 tatccagtct atgtcatcaa ctacaagggt gaggaggtct gtctggctca ggctcctgtt
      961 ggctccgctc cagcagccca gtttatggat tggttgattg gctatggtgt ggagcagatt
     1021 atctctactg ggacctgtgg tgtcctagct gatatagagg aaaatgcctt tctagtccct
     1081 gttcgcgctc tgcgagatga aggagccagt taccactatg tggcaccttc tcgttatatg
     1141 gaaatgcagc cagaggctat tgctgccatt gagcgagttt tggatgatag agggattcct
     1201 tatgaagaag tcatgacctg gtccacagat ggtttttacc gagaaacggc tgaaaaggtg
     1261 gcttatcgta aggaagaagg ctgtgctgtt gtggagatgg agtgttctgc tcttgcggca
     1321 gtagctcaat tgcgtggagt tctctggggt gaattgttgt tcacagcaga ttctctagcg
     1381 gacttggacc agtacgacag tcgtgactgg ggctcggaag cttttaataa ggcactagaa
     1441 ctgagtttag caagtgttca ccacctttag ttgtactggc aaaggatttg ttttatcata
     1501 aaatgtctag ctcatacttt tcaaaaatat gtttaaacga ggtcaccttc ctcttgtcct
     1561 aggcatgttg aggttgggaa aaatctttaa aatcagaaaa acgtatcata tcaggtgatg
     1621 aaaattttga tactatgcgt tttatgttgg taagatttag agtgagatga aatgatactc
     1681 ttcgaaaatc tcttcaaacc aggtcagctt caccttgccg tatagatgtt actgacttcg
     1741 tcagttctat ccacaacctc aaaacagtgt tttgagcaac ctgtgactag ctttctaagc
     1801 gatgccttgg ttctcattgc ctataatcaa aaagagaaat tttctcctga aaagcatata
     1861 gagtagctgg cgttaaaagc tcctgtcttg cttttttgac ctatagtcac atctatcaag
     1921 tattgttctt gcctaagcta tcaataaaaa ggtggcattt tttaggcttg gtgttagtag
     1981 attttgcctt atcctatcta agtcatttcg aactttttat ggtacaatgg aaacatgtta
     2041 ttcaaattat ctaaggaaaa aatagagcta ggcttatctc gtttatcgcc agcccgtcgt
     2101 atttttttga gttttgcctt ggtcatttta ctaggctctc ttcttttgag cttgcccttt
     2161 gtccaagttg aaagctcacg agcgacttat tttgatcatc ttttcactgc tgtctctgca
     2221 gtctgtgtga caggtctctc aacccttcca gtagctcaca cctataatat ctggggccaa
     2281 ataatctgtt tgctcttgat tcagatcggt ggtctagggc tcatgacctt tattggggtt
     2341 ttctatatcc agagcaagca aaagcttagt cttcgtagcc gtgcaactat tcaggatagt
     2401 tttagttatg gagaaactcg atctttgaga aagtttgtct attctatttt tctcacgacc
     2461 tttttggttg agagcttggg agctattttg cttagttttc gccttattcc tcaacttggc
     2521 tggggacgtg gtctttttag ttccattttt ctagcgatct cagccttctg taatgccggt
     2581 tttgataatt tagggagcac cagtttattt gcttttcaga ccgatttact ggtcaatctg
     2641 gtgattgcag gcttgattat tacaggcggc cttggtttta tggtctggtt tgatttggct
     2701 ggtcatgtag gaagaaagaa aaaaggacgt ctgcactttc atacgaagct tgtactatta
     2761 ttgactatag gtttgttgtt atttggaaca gcaactactc tctttcttga gtggaacaat
     2821 gctggaacga ttggcaatct ccctgttgcc gataaggttt tagttagctt ttttcaaaca
     2881 gtgacgatgc gaacagctgg cttttctacg atagattata ctcaggctca tcctgtgact
     2941 cttttgattt atatcttaca gatgtttcta ggtggggcac ctggaggaac agctggggga
     3001 ctcaagatta cgacattttt tgtcctcttg gtctttgcac gaagtgagct tctaggcttg
     3061 cctcatgcca atgttgcgag acgaacgatc gcgccgcgaa cggttcaaaa atcctttagt
     3121 gtctttatta tctttttgat gagcttcttg ataggattga ttctgctagg gataacagcc
     3181 aaaggcaatc ctccctttat ccacctcgta tttgaaacca tttcagctct tagtacagtt
     3241 ggtgtaacgg caaatctgac tcctgacctt gggaaattgg ctctcagtgt tatcatgcca
     3301 cttatgttta tgggacgaat tggtcccttg accttgtttg ttagcttggc agattaccat
     3361 ccagaaaaga aagatatgat tcactatatg aaagcagata ttagtattgg ttaagaaagg
     3421 aaagagcatg tcagatcgta cgattggaat tttgggcttg ggaatttttg ggagcagtgt
     3481 cctagctgcc ctagccaagc aggatatgaa tattatcgct attgatgacc acgcagagcg
     3541 catcaatcag tttgagccag ttttggcgcg tggagtgatt ggtgacatca cagatgaaga
     3601 attattgaga tcagcaggga ttgatacctg cgataccgtt gtagtcgcga caggtgaaaa
     3661 tctggagtcg agtgtgcttg cggttatgca ctgtaagagt ttgggggtac cgactgttat
     3721 tgctaaggtc aaaagtcaga ccgctaagaa agtgctagaa aagattggag ctgactcggt
     3781 tatctcgcca gagtatgaaa tggggcagtc tctagcacag accattcttt tccataatag
     3841 tgttgatgtc tttcagttgg ataaaaatgt gtctatcgtg gagatgaaaa ttcctcagtc
     3901 ttgggcaggt caaagtctga gtaaattaga cctccgtggc aaatacaatc tgaatatttt
     3961 gggtttccga gagcaggaaa attccccatt ggatgttgaa tttggaccag atgacctctt
     4021 gaaagcagat acctatattt tggcagtcat caacaaccag tatttggata ccctagtagc
     4081 attgaattcg taaagaggga tgacccctct tttttgatgc ctaagatggc aaatagagac
     4141 agaagcccct tgtcttctag taaaagttct tcaaaggctg gactttatgg taaaatagaa
     4201 agaagtgaca agagagagta atactcaatg aaaatcaaag atcaaactag gaaactagct
     4261 acgggctgct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagctcaaaa
     4321 cactgttttg aggttgcaga tagaactgac gaagtcagta acatctatac ggcaaggcga
     4381 cgttgacgcg gtttgaagag attttcgaag agtataagaa aaaatcagtc ccctaaagga
     4441 gtagattatg aagttattgt ctatcgcaat ttctagctat aatgcagcag cctatcttca
     4501 ttactgtgtg gagtcgctag tgattggtgg tgagcaagtt gggattttga ttatcaatga
     4561 cgggtctcag gatcagactc aggaaatcgc tgagtgttta gctagcaagt atcctaatat
     4621 cgttagagcc atctatcagg aaaataaatg ccatggcggt gcggtcaatc gtggcttggc
     4681 agaggcttct gggcgctatt ttaaagtagt tgacagtgat gactgggtgg atcctcgtgc
     4741 ctacttgaaa attcttgaaa ccttgcagga acttgagagc aaaggtcaag aggtggatgt
     4801 ctttgtgacc aattttgtct atgaaaagga agggcagtct cgtaagaaga gtatgagtta
     4861 cgattcagtc ttgcctgttc ggcagatttt tggctgggac caggtcggaa atttctccaa
     4921 aggccagtat accatgatgc actcgctgat ttatcggaca gatttgttgc gtgctagcca
     4981 gttctaactg cctgaacata ctttttatgt cgataatctc tttgtcttta cgccccttca
     5041 gcaggtcaag accatgtact atctgcctgt cgatttctat cgttatttga ttgggcgtga
     5101 ggaccagtct gtcaatgagc aagtgatgat taagtgcatt gaccagcaac tcaaggtcaa
     5161 tcgactcttg atagaccaac ttgatttgtc ccaagtgagt catcccaaaa tgcgagaata
     5221 tctgctgaat catattgaac tcacgacggt gatttccagt accctgctca accgatctgg
     5281 aacagcggag catctggcaa aaaaacgcca attgtggacc tatattcagc agaaaaatcc
     5341 agaagtcttt caggctattc gtaagaccat gttgagccgt ttgaccaaac attctgtctt
     5401 gccagatcgc aaactgtcca atgtcgtcta tcaaatcacc aaatctgttt atggatttaa
     5461 ttaatataag tgttttataa gagggattta agaaaaattt taactttttc ttagtccttt
     5521 ttaatttcag gagattatac tagagtcatc aaataaagaa agactctaag gagaatccta
     5581 tgaaattcaa tccaaatcaa agatatactc gttggtctat tcgccgtctc agtgtcggtg
     5641 ttgcctcagt tgttgtggct agtggcttct ttgtcctagt tggtcagcca agttctgtac
     5701 gtgccgatgt ggtcaatccg accccaggtc aagtcttacc tgaagagaca tcgggaacga
     5761 aagagggtga cttatcagaa aaaccaggag acaccgttct cactcaagcg aaacctgagg
     5821 gcgttactgg aaatacgaat tcacttccga cacctacaga aagaactgaa gtgagcgagg
     5881 aaacaaactc ttctagtctg gatacacttt ttgaaaaaga tgaagaagct caaaaaaatc
     5941 cagagctaac agatgtctta aaagaaactg tagatacagc tgatgtggat gggacacaag
     6001 caagtccagc agaaactact cctgaacaag taaaaggtgg agtgaaagaa aatacaaaag
     6061 acagcatcga tgttcctgct gcttatcttg aaaaagctga agggaaaggt cctttcactg
     6121 ccggtgtaaa ccaagtaatt ccttatgaac tattcgctgg tgatggtatg ttaactcgtc
     6181 tattactaaa agcttcggat aatgctcctt ggtctgacaa tggtactgct aaaaatcctg
     6241 ctttacctcc tcttgaagga ttaacaaaag ggaaatactt ctatgaagta gacttaaatg
     6301 gcaatactgt tggtaaacaa ggtcaagctt taattgatca acttcgcgct aatggtactc
     6361 aaacttataa agctactgtt aaagtttacg gaaataaaga cggtaaagct gacttgacta
     6421 atctagttgc tactaaaaat gtagacatca acatcaatgg attagttgct aaagaaacag
     6481 ttcaaaaagc cgttgcagac aacgttaaag acagtatcga tgttccagca gcctacctag
     6541 aaaaagccaa gggtgaaggt ccattcacag caggtgtcaa ccatgtgatt ccatacgaac
     6601 tcttcgcagg tgatggtatg ttgactcgtc tcttgctcaa ggcatct
//
LOCUS       DAVLYR010000099         6546 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLYR010000099 DAVLYR010000000
VERSION     DAVLYR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.099"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            7..870
                     /locus_tag="VYO63_001500"
     CDS             7..870
                     /locus_tag="VYO63_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1672397.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1253..1873
                     /locus_tag="VYO63_001501"
     CDS             1253..1873
                     /locus_tag="VYO63_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672398.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1959..2402
                     /gene="tsaE"
                     /locus_tag="VYO63_001502"
     CDS             1959..2402
                     /gene="tsaE"
                     /locus_tag="VYO63_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1672399.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2392..2910
                     /locus_tag="VYO63_001503"
     CDS             2392..2910
                     /locus_tag="VYO63_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672400.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2918..3934
                     /locus_tag="VYO63_001504"
     CDS             2918..3934
                     /locus_tag="VYO63_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1672401.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4019..5275
                     /locus_tag="VYO63_001505"
     CDS             4019..5275
                     /locus_tag="VYO63_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1672402.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5330..6496
                     /gene="recA"
                     /locus_tag="VYO63_001506"
     CDS             5330..6496
                     /gene="recA"
                     /locus_tag="VYO63_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1672403.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
BASE COUNT         2071 a         1078 c         1483 g         1914 t
ORIGIN      
        1 tctttaatga atacactcgc agagaaattc agattgaaaa gaaaagagtt gagactctcc
       61 caacaaactc ttgcagaagg aatttgtgaa caaagccaga ttagtaaaat tgaaagaggt
      121 cattttattc cctccgcaga cttgttattc aaactctctc aacgacttga agtccctttg
      181 gattactttt tcaatgaaca aatcgaagtt aagtctaacc tctctaattt caaacatcta
      241 tctgctcgcc tattagatga cagaaattat gacgatttag aatatcttta taaaatagag
      301 gtagaacgaa gcacttttct aacattagaa gaccgaactt accttgaatg gattaaatct
      361 ataattgact tctatcaata tgacagtaag tgtgaggcaa tttcgtcatt ggaaaatata
      421 ttattaaaag tctcttcaaa tactctgatt tatttaaagg cgttgaatac tctatctaat
      481 ttctattcct tagtgggtcg tgaacaagaa tatgaggcaa actactctca tttaatagag
      541 ttgtatcaga caaaaaattt ggaccatcaa gagtttttat ttggctacat cagagttcgt
      601 tacaactacg ctcactacct agtatcaaag gaaaaatata atgaagccat ccaagaagct
      661 cttgaaacga ttgaactctg taaacaaaga cagacaagct accaattggc tcctctactt
      721 attcttgtag gaaatgctgg agccaaattt ctagacagag aacaagtcaa aaattattat
      781 atagaagcaa gagagttatg taagatttat aacaatcctt taatgttgat gaagatagaa
      841 aattatttga aggaactaga tactgtttag ttaatcttga cattatagtt tttctgaaac
      901 gttaaataac ctgtaaggct gatagttaga ttaatactat cagccttata attatgtgac
      961 tctgagtccg tctcgctccg tgaggtgcga gacaaaacaa tcacccgttt gacggatttt
     1021 catgatttca aatttatatt cttcgaaaat ctcttcaaat cacgtcagct ctatctgcaa
     1081 tctcaaaaca ctgttttgag caacctgtgg ctagctacct agtttgatct ttgattttca
     1141 ttgagtatta gtctctagtg tttgtttagc gtcttaaaga gacatttgag aagattagaa
     1201 acagttctct cttttctcct agtataatga ttggtaggaa gggagagaga agatgaaatc
     1261 aatgagaatc ttatttttgt tagctttaat tcaaatcagt ttgagtagct gtttcctatg
     1321 gaaggaatgc atcttgtcct ttaaacaaag tacagctttt ttcatcggaa gcatggtttt
     1381 cgtttcagga atctgtgctg gagtaaatta tctttatacc cgtaagcaag aagtccatag
     1441 tgtcctagcc agtaagaagt cggtgaagct tttttacagt atgttactct taattaattt
     1501 gttaggagct gttcttgttt tgtcagataa cttgttcatc aaaaatacgc tgcagcaaga
     1561 attagttgac tttttattgc catccttctt tttcctattt gggctagatt tgctgatttt
     1621 tttacccttg aaaaaatacg tgcgcgattt tcttgctatg ctggacagaa aaaagacagt
     1681 gttggtgact attttagcaa cacttctttt cttaagaaat ccaatgacca ttgtctcact
     1741 tctgatttat attggactgg gcttgttttt tgcagcctat cttgtcccaa attcggttaa
     1801 gaaggaagtt tccttttatg gtcatatttt ccgagatctt gtattggtca ttgttacgct
     1861 cattttcttt tagagaaagc ctgtttttta tggatgttat gatgataagt ttagtaggaa
     1921 aatagacaca aaagaaaagt ttttggtata ataagagtat gtacacaaaa aatgaagaag
     1981 agttgcaagc cttaggggag cgtttgggcc atctattagc aaagaatgat gttttaatct
     2041 taactggaga actgggtgca ggtaaaacga cctttactaa aggacttgca aaaggattac
     2101 agatttctca aatgattaaa agtcccacct atactatcgt gagagagtat gaaggtcgac
     2161 ttccacttta tcacctagat gtttatcgta ttgaaggaga tgctgattct atcgacttgg
     2221 atgagtttat ctttggtggc ggcgtgactg ttattgagtg gggaaatctc ttaggagatg
     2281 ccttgccaga tgcttattta gaattggaaa ttctaaaaga agcagatgga cgccgtttaa
     2341 attttcaggc aaagggtttg cgtgctgaga aattgttaga ggagcttcaa tatggagtat
     2401 gaattgctca ttagggaagc agagcccaaa gatgcagctg aattagtggc ctttttaaat
     2461 cgtgtgagtt tggagacaga ctttaccagc ctagacggag atggtattct cttgactagt
     2521 gaggagatgg aaatattcct caacaagcaa gctagttcgg acaatcagat aaccttactt
     2581 gcattcttaa atggcaaaat tgctggtatt gtaaatatta cagctgatca gcgtaagaga
     2641 gtccgtcata ttggagatct cttcattgtg attggaaaaa gatattggaa taatggcttg
     2701 ggaagtttgt tgctagaaga agcgatagag tgggcacaag caagtggcat tctgcgtcgt
     2761 ctccaactga ctgtccaaac tcgtaatcaa gcagcagtcc atctttatca aaagcatggc
     2821 tttgtcattg aaggtagcca agagcgtggg gcatatatag aagaagggaa atttatcgat
     2881 gtttacttga tgggtaaact gataggttag tagaaatatg gttaaaaaaa ttattggaat
     2941 ggtgctagct ttactttctg taactgtagt aggagtaggt gtttttgctt atactattta
     3001 tcaacaaggg acagaaacct tagctaaaac ctataaaaaa atcggtgaag aaaccaaggt
     3061 tattgaagcg actgaacctc taaccattct gttaatggga gtggacaccg gaaatgttga
     3121 acgaactgaa acttgggtcg gtagaagtga tagcatgatc ttgatgacag tgaatcctaa
     3181 aacgaaaaaa acaacaatga tgagtttaga gcgggatatt ctgacgcgca ttgaatcagg
     3241 gaatggtcag gctcatgaag cgaaactgaa ctcagcatat gcagatggtg gagcagagct
     3301 tgctatagaa accattcaaa aaatgatgaa tatccatatt gatcgctatg tgatggtcaa
     3361 tatgagaggg ttgcaaaaat tagtggatgc agtaggaggt attacagtca ataatatcct
     3421 aggtttccca atttctatca gtgaccaaga agaatttaat accatttcta tcggtgttgg
     3481 ggagcaacat attgggggag aagaagccct agtctatgca cgaatgcgtt accaagatcc
     3541 tgagggggat tatggtcgtc aaaaacgtca acgtgaagtt attcaaaaag tcatggaaaa
     3601 agctctcagt ttaaatagca ttggtcatta tcaagagatt ctaaaagctt tgagtgacaa
     3661 tatgcagacc aatattgatt tgtctgcaaa aagtatccct aacttgctag gctataaaga
     3721 ttcatttaaa accattgaaa ctcagcagtt gcagggtgaa ggagagatac ttcaaggtgt
     3781 ttcttaccag attgtttcga gagcacatat gttggaaatg caaaatatac tccgacgttc
     3841 tttgggacaa gaagaagtta ctcagcttga aaccaatgcg gttttatttg aagatttatt
     3901 tggcagagca cctgttggtg atgaagataa ttaacttttg atacaaataa aaaaatcaat
     3961 cgtaggaaaa ttcctgcgat tttttcaaaa aaatacgaat agataggtag gaggaaacat
     4021 gaaagcagaa atcattgctg ttggaacaga gattttgaca ggacagattg tcaacaccaa
     4081 tgcccagttt ttgtcggaaa aactagctga gattggggta gatgtatatt ttcagacggc
     4141 tgtaggagac aatgaagttc gtctcttgtc tttgcttgag attgccagtc aacgtagcag
     4201 tctggtgatt ttgacaggcg gtttggggcc aactgaggac gacctaacca aacaaaccct
     4261 agctaaattt ttagggaaag cattagtctt tgatcctcag gctcaggaga agttggatat
     4321 cttttttgcc ctgcgaccag actatgcccg aacaccgaat aacgaaagac aagctcaaat
     4381 tgtagaagga gcgattccac tgccaaacga aacaggactg gctgttggag gaaaattaga
     4441 agtagacgga gtgacctatg tcgtccttcc aggtccgcca agtgaattga aacccatggt
     4501 cttaaaccaa cttctaccca agttgatgac agggagcaag ctgtattccc gagttcttcg
     4561 tttctttggg attggcgaga gccagttggt tacgattttg gctgatttaa ttgataatca
     4621 gatcgatcct accttggccc cttatgccaa gacaggagaa gtcactctac gtctgtcaac
     4681 aaaggctagc agtcaagaag aggcgaatca agcgctggat atcttggaaa atcaaatctt
     4741 ggactgccag actttcgaag gaatttcttt acgagacttt tgctatggtt atggggaaga
     4801 aactagttta gccagcattg tggtagaaga actgaaaagg caagggaaaa ccatcgcggc
     4861 tgcagagagt ttgacggcag gtcttttcca agctaccgtg gcgaattttt ctggagtttc
     4921 aagtatattt gagggtggtt ttgtgaccta tagcttggag gaaaaatcaa ggatgttgga
     4981 tattcctgcc aagaatttgg aagaacatgg tgtggtgtct gaatttacag ctcagaagat
     5041 ggctgagcag gcacgaagca agacccagtc tgattttgga attagtttga ctggagtggc
     5101 aggaccagat agcctagaag gacacccagt tgggacagtc ttcataggct tggcgcaaga
     5161 tcaaggaact gaggttatca aggttaatat tggaggcaga agccgagcag atgtacgtca
     5221 cattgcggtt atgcatgcct ttaacctagt tcgcaaggct ttattaagtg actaactttt
     5281 gatataatag tagataggtc tgaggatcat tagaatgtag gagaatagaa tggcgaaaaa
     5341 accaaaaaaa ttagaagaaa tttcaaaaaa atttggggca gaacgtgaaa aggccttgaa
     5401 tgacgctctt aaattgattg agaaagactt tggtaaagga tcaatcatgc gtttgggtga
     5461 acgtgcggag caaaaggtgc aagtgatgag ctcaggttct ttagctcttg acattgccct
     5521 tggctcaggt ggttatccta agggacgtat catcgaaatc tatggcccag agtcatctgg
     5581 taagacaacg gttgcccttc atgcagttgc acaagcgcaa aaagaaggtg ggattgctgc
     5641 ctttatcgat gcggaacatg cccttgatcc agcttatgct gcggcccttg gtgtcaatat
     5701 tgacgaattg ctcttgtctc aaccagactc aggagagcaa ggtcttgaga ttgcgggaaa
     5761 attgattgac tcaggtgcag ttgatcttgt cgtagtcgac tcagttgctg cccttgttcc
     5821 tcgtgcggaa attgatggag atatcggaga tagccatgtt ggtttgcagg ctcgtatgat
     5881 gagccaggcc atgcgtaaac ttggcgcctc tatcaataaa accaaaacaa ttgccatttt
     5941 tatcaaccaa ttgcgtgaaa aagttggagt gatgtttgga aatccagaaa caacaccggg
     6001 cggacgtgct ttgaaattct atgcttcagt ccgcttggat gttcgtggta atacacaaat
     6061 taagggaact ggtgatcaaa aagaaaccaa tgtcggtaaa gaaactaaga ttaaggttgt
     6121 aaaaaataag gtagctccac cgtttaagga agccgtagtt gaaattatgt acggagaagg
     6181 aatttctaag actggtgagc ttttgaagat tgcaagcgat ttggatatta tcaaaaaagc
     6241 aggggcttgg tattcttaca aagatgaaaa aattgggcaa ggttctgaga atgctaagaa
     6301 atacttggca gagcacccag aaatctttga tgaaattgat aagcaagtcc gttctaaatt
     6361 tggcttgatt gatggagaag aagtttcaga acaagatact gaaaacaaaa aagatgagcc
     6421 aaagaaagaa gaagcagtga atgaagaagt tccgcttgac ttaggcgatg aacttgaaat
     6481 cgaaattgaa gaataagctg ttaaagcagt ggagaaatcc gctacttttt cgatttttga
     6541 ttcaag
//
LOCUS       DAVLYR010000100         6535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLYR010000100 DAVLYR010000000
VERSION     DAVLYR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.100"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            75..635
                     /locus_tag="VYO63_001507"
     CDS             75..635
                     /locus_tag="VYO63_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1672404.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            636..2021
                     /locus_tag="VYO63_001508"
     CDS             636..2021
                     /locus_tag="VYO63_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672405.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            2023..2673
                     /locus_tag="VYO63_001509"
     CDS             2023..2673
                     /locus_tag="VYO63_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1672406.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            2684..3376
                     /gene="tenA"
                     /locus_tag="VYO63_001510"
     CDS             2684..3376
                     /gene="tenA"
                     /locus_tag="VYO63_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1672407.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            3381..3905
                     /gene="thiW"
                     /locus_tag="VYO63_001511"
     CDS             3381..3905
                     /gene="thiW"
                     /locus_tag="VYO63_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1672408.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            3905..4708
                     /locus_tag="VYO63_001512"
     CDS             3905..4708
                     /locus_tag="VYO63_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1672409.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLNYRPTVIKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            4701..5333
                     /locus_tag="VYO63_001513"
     CDS             4701..5333
                     /locus_tag="VYO63_001513"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1672410.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(5542..6333)
                     /gene="thiD"
                     /locus_tag="VYO63_001514"
     CDS             complement(5542..6333)
                     /gene="thiD"
                     /locus_tag="VYO63_001514"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1672411.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQAKDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(6417..6515)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1781 a         1310 c         1432 g         2012 t
ORIGIN      
        1 ttcaatctgc tatacaaagt atttaggtcg gtcttcttga gtgccccact tttcttttaa
       61 aaaaggagtt ttttatgttg aaaaaatggc agttaaaaga tgttatcttg cttgctttct
      121 tgtctatctt ttttggtggg gttttcgttg gttcaggata tgtgtataat attctcagtc
      181 tactcttaac acctcttggt ttgcaggcct ttgccaatga aatcctcttc ggtctctggt
      241 gtatggctgc gcccattgct gccatctttg ttccgagagt cggaagtgca acgattggag
      301 aagtgctagc tgcgcttgct gaagtccttt atggtagcca atttggtcta ggagctcttt
      361 tgtctggctt ggttcaaggt ttgggaagtg aatttggttt tatcgtaact aagaatcgct
      421 atgaaagttg gctctctcta actgctaata gtattgggat tacgcttgtt agctttgtct
      481 atgaatacat taagttaggt tactacgcct tttcccttcc gtttgtcctt tccttacttg
      541 tggtacgttt tatttctgtt tatttcttct gtaccatctt ggttcgtgcc attgtcaaac
      601 tctatcatca gtttgcaact ggaggaaaag catagatggg tctggaacta cgagcgattc
      661 agtccccaat cttctctgag ccgtttgatt ttacttttca tgcgcaagcc tttaccttgt
      721 tagttgggag cagtggttca ggaaaatcca gcctttttca agtcattgcc caagtcagtt
      781 ctcttcccta tagtggtcaa gtcctgatag atgggagcga ggtcagtcag ctttctatca
      841 tcgcacgtgt ccagaaggtt ggcattctct ttcaaaatcc caatcatcaa tttaccatgg
      901 agaacttgtt tgaggagctg attttcacct tggagaatat cggctatcac cttcaggaaa
      961 ttgattctaa aatagcagag gttgtccagc aatgtcgttg cgaggcaatc ttacaccgtc
     1021 ccattcatca cctatcaggt ggggaaaagc aaaaagcagc gctggctgtc ctctttgcca
     1081 tgaatcctag ggtctatctc ttggatgagc ccttcgcttc tattgaccgc aagagcagaa
     1141 tcgagatatt ggagattcta aaagagttgg ctcttgatgg gaagacagtt attttgtgcg
     1201 accatgattt atctgactat aaagcctata tcgaccatat ggttgagcta agagacggaa
     1261 aactaaggga agtgtttcaa atcccttcct atgagatgac acaggttgct tcaaaggaag
     1321 ttgcttctag cccggaacta ttccatatga accgtgtgac tggtgagctt ggtaatcgcc
     1381 ccctcttttc aattgctgat ttcacattct atcaagggat ttcctgtatc ctgggtgaca
     1441 atggtgtcgg gaaatcaacc ctctttcgct ctattcttca atttcaaaag tataaggggc
     1501 gcattgcatg gaaggggaca gtcctgaaaa agaaaaagag tttgtatcgt gatctgactg
     1561 gtgttgttca ggaagctgag aagcagttta tccgagtcag tctgcgagag gagcttcaat
     1621 tagatggacc tgattctgaa agaaatcagc ggatttttca agctttacga tattttgatt
     1681 tggagcaggc agtcgataag agtccctatc aattaagtgg tggtcagcaa aaaattcttc
     1741 agctcttgac catcttgacc agtaaggctt ccgtgatctt gctagatgaa ccttttgcag
     1801 gtttggatga tagagcctgc cattattttt gcaagtggat tgtggaggag aggaatcaag
     1861 gaagaagttt tctgctcatt agtcatcgtt tagacccttt gatttctgtg gttgattatt
     1921 ggattgagat gactagtcag ggtcttcggc atgtgaaaga agtgaccatt accaaaccac
     1981 ttacatctca gagtagcaat acccaagggg aggtgagata gtatggtcaa agtagcaacc
     2041 cagacaccga ttatcagtct cttcttgctg attttatcct tggaaacatc tttcattcct
     2101 tcgattgctc tgaatctttc ggtagtcgca ttttgtattc tctttatgct ctattaccgt
     2161 cgatttaaaa tgttagcttg gatgatcata cttgccattt taccatcttt tgccaactac
     2221 tgggcagttc agttacacgg agatgcttca caggcagtca tgcttggaac gagggccttt
     2281 gtgacagttt gtatcggcct tgtctttgtt tcctctattt cactaaaaga gcttctcttg
     2341 tacttggctc aaaaggggct atcacgctct tggtcctatg ccttgattgt ggtattcaat
     2401 tcctttcctc tcattcagca agaaatcaag tccctcaaag aagcttgcct attacgtggt
     2461 caagaactgc atttttggtc gcccttgatt tacagtaagg ttctgatgac agtctttagg
     2521 tggcgccatc tttacctgag agctctgtct gctcatggat atgacgaaca tgcacagttg
     2581 aagaatagct atcggacttt ttatattcct aaaaaaacaa aattaatcta cctgcttttc
     2641 tttttattgc ttcaaaccag tctattttta taaaggagtt attatggaat ttacagatat
     2701 tgcgatggaa ttatccaaga aagcttggca ggcttccttt catcatccct ttattttaca
     2761 attacaagag gggaatttag aacctgccat tttccgctat tacctgattc aggatgccta
     2821 ctatctgaag gccttctcag aaatctatca tcttttggct gataagactt caaaccaaga
     2881 gatgaaaaga ctcttgaaac aaaatgctca gggtttagtg gagggtgagt tatttattcg
     2941 ccaacaattt ttcaaggaaa tggaaatcag cgaccaggaa atggagcaac atccaattgc
     3001 tccaacctgt tatcattaca tttctcatat ttataggcaa tttgcagaac cgaacttagc
     3061 catagctttt gcaagcttgc ttccttgtcc ttggttatac catgatatag gcaaatcact
     3121 taatcttaaa ccatcaccaa atcctctcta tcaacaatgg attgaaactt atattacgga
     3181 tgagttagag caacagatca gagaggaggg agcactggtc aatcagctct atcgagaaag
     3241 tgatgagaca gataagcaaa aaatgctaga tgccttccac atcagtgttc atatggaagc
     3301 caagttttgg gagatggctt accaacacca gacatggaag agcgatttac agtctttaga
     3361 aaaaggagaa gaatagaaac atgagaaagc accaactaca agttcacaaa ttgaccattt
     3421 tatctatgat gattgccctt gatgtagtcc ttacacctat ctttcgaatt gagggaatgg
     3481 caccgatgtc cagtgtagtc aatattctag cgggaatcat gatgggacct gtttatgcct
     3541 tggccatggc tacagtcaca gcctttatcc gtatgacgac tcaagggatt ccgcctttag
     3601 ctctcacagg agcgactttt ggagcccttc tggcaggtct cttttataag tacggtcgaa
     3661 aattttattt ttctgccttg ggagaaattg tgggaacagg tattattggt tccattgttt
     3721 cctatcctgt tatggtactc tttacaggat cagctgctaa gcttagctgg tttatctaca
     3781 cgcctcgatt tttcggagca accttgattg gtacagcgat ttcctttatt gcctttcgat
     3841 ttttaatcaa gcaggaattc tttaaaaaag tgcagggata tttctttagt gaaaggatag
     3901 actgatgcag gaatttacaa atccctttcc tataggctct agttccctaa ttcactgtat
     3961 gaccaatgag atttcttgtg agatgctggc aaatgggatt ttggctctgg gatgcaaacc
     4021 tgtcatggca gatgatcccc gtgaggttct tgattttact aagcaaagtc aggctctttt
     4081 catcaatttg gggcatttgt cagctgagaa ggaaaaagca atccgcatgg cagcttcgta
     4141 tgcaaaccaa tcttctctcc cgatggtagt agatgcggtt ggcgtaacga cttcatccat
     4201 tcgtaagagc ttagttaaag accttttaaa ctatagacct acggtcatta aaggaaacat
     4261 gtcagaaatt cgaagtcttg ttggcttaaa gcaccacggc gttggggtcg atgcgagtgc
     4321 taaagatcaa gaaacggagg atttgcttca agtcttgaaa gactggtgtc agacctatcc
     4381 tggtatgtca ttcttagtta ctggccccaa ggacctcatc gtttcgaaaa atcaggtcgc
     4441 tgtactggag aatggctgta ctgaattaga ctggataaca gggacaggag atttggttgg
     4501 agccttaaca gccgtttttc tcagccaagg aaagactggt tttgaagctt cttgcttagc
     4561 agtctcttat ctcaatatcg ctgctgagaa aatagttgtt caaggaatgg gattggaaga
     4621 atttcgttac caagtactca atcagctttc gctcctaaga agagatgaaa attggctaga
     4681 taccatcaaa ggagaggctt atgaatagag aagcacttag actatatctg gtaaccaatc
     4741 gctatcaaga ttccgtggaa agctttcttg caaaagttga gacggcctgc cgttcagggg
     4801 ttaccatagt ccaattgcga gaaaaaaatc tcacaaccaa tcaatattat caactggcaa
     4861 aacaagtcaa ggaaataaca gatgcctatc aggtaccctt gataatcgat gatcggttgg
     4921 atgtttgtct tgcggttgat gctgctggtc tgcatattgg agatgacgaa ctcccagttt
     4981 cggttgcccg acaagtcttg ggtcctgaaa aaatcctcgg tgtcaccgct aaaacggtta
     5041 aaagagctct ggaagcggag aaatcaggtg cagattactt ggggacagga gccatttttc
     5101 cgacaaccac caaggaaaat gcacccatca ccctgatttc aaccttgaaa acaatttgcc
     5161 aaacggttgc cattccaata gttgctattg gcggcttgac ttcggagaat attgatcagc
     5221 ttatgggcac tggcatagct ggtgtagctg ttgtacgtga tttgatgcaa gcagaagata
     5281 ttgaggcaaa aacgcaagcc tttttaacaa agttgcatga cattctttcc taattaatac
     5341 tcaatgaaaa tcaaagagta aactagaaag ctagtcgcag gttgctcaaa gtactgcctt
     5401 gaggttttag ataagactga cgaagtcagc tcaaaacagt gttttgaggt tgcagatgga
     5461 agctgacgta gtttgaagag attttcgaag ggtataaaaa ctctctaatt cccttaaagt
     5521 gggaactaga gagttttttt cttaatcttt aaaagttgta tggttgactg gaccagaacc
     5581 atgaccgagt tgaggggcat cttgaatagc ttttgtgata aaggccttgg ccttatcaac
     5641 tgcctggtaa agactcttgc ccttggctag ttcagcagta atcactgcag caaaggtaca
     5701 tccagtacca tgggtgtgac aggtttgaat tcgtgggctt tcccagacaa attgttcatt
     5761 cttggtaaag aggaaatctt tagcaccacc tttgagatgt ccgcctttga taaccacaga
     5821 ctgaggacca aattctttta aaatcaggcg accagcacgc tgcatgtctt cggggtcatg
     5881 gattgaaaaa ccaacaatct cttctgcttc aggaagattt ggcgtaataa tagttgctag
     5941 aggtagtaag tttgttttga gatagtctct agcatttgag tcaatcaagg catctccact
     6001 tgtagcaacc ataacaggat caaggacata gggacaatcc agttttttaa gatagggttg
     6061 gatgatttcc atgatttcag tagtagccaa cattccagtt tttacagcct gaggtggaat
     6121 atcagaaaag acactctcca attgggcttt caacatttga ggagaaacgt gctcgattag
     6181 ctgaacacct ctggtatttt gagcgacaag actggttaca acagccattc catagacatc
     6241 tttcgcttgg aatgacttta aatctgccat aatgccagca ccaccactag ggtcagtccc
     6301 tgcaatggtc aaagcaacgg gtaaataagt catctaaaac ctcccaacca actgaagttt
     6361 gtattcttaa gaacaagcta gtctgtttta gaaaggcaat agaagactgc tagtccccat
     6421 tatcatttcc acttccatca gctagcatta cctagattga gtcagaggga tctaacagtc
     6481 gttaatctca gctttacgca cccctagtgg ttgttttctt ttttctttag tatag
//
LOCUS       DAVLYR010000101         6491 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLYR010000101 DAVLYR010000000
VERSION     DAVLYR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.101"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(80..1276)
                     /locus_tag="VYO63_001515"
     CDS             complement(80..1276)
                     /locus_tag="VYO63_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1672412.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(1366..6345)
                     /locus_tag="VYO63_001516"
     CDS             complement(1366..6345)
                     /locus_tag="VYO63_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1672413.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
BASE COUNT         1681 a         1459 c         1209 g         2142 t
ORIGIN      
        1 ttgagaggaa gcgtttttat tttaagcaaa agaaaagagg aactttcatc cctctcttct
       61 ttgatttgtt tataaaatct tatttttctg tcagggctgc aagtcctgga agaaccttac
      121 cttcaagaag ttccattgat gctccaccac ccgtactaat ccatgagaac ttgtctgcac
      181 ggccaaggtt aatcgctgcg gcagctgagt caccaccacc gatgattgat ttaactcctg
      241 gttgtttcac gatagcgtcc atcacaccga ttgtaccagc ttggaaatct gggttttcaa
      301 atacacccat aggtccgttc catacaactg ttttggcacc agtcaaagct tcgtcaaatt
      361 tggcgataga ttttggaccg atgtcaagac caaggaagcc ttcagaaact gcttcacctt
      421 cagtgtcacg cacttcagtg taaccagcaa atgcgttagc ttcttttgag tcaactggca
      481 agatcaattt accatttgct ttttcaagaa gagctttcgc aacatccaat ttgtcttctt
      541 ctacaagtga gttaccgatt tcgatacctt gtgctttgta gaatgtgtaa gtcatcccac
      601 caccgataag gactttatca gctttttcaa gcaagttttc gataacaccg atcttgtctg
      661 aaacttttga accaccaagg atagccacga atggacgttc tggagtttca actgcttctt
      721 ggatgtaggc aatttcgttt tcaagaagga aaccagcaac tgctttttca acgtttgctg
      781 agataccaac gttagatgcg tgtgcacggt gagctgtacc gaatgcatcg tttacgaaga
      841 taccatctcc aagtgatgcc cagtatttac caagttcagg atcgttttta gattctttct
      901 tgccgtcaac atcttcgtaa cgagtgtttt caaccaagag aacttgtcca tcttcaagag
      961 cgttgattgc cgcttccaat tcagcaccac gagtgacacc tgggaaaaca acatcttgac
     1021 caagttttgc tgccaagtca gctgctacag gagcaagtga tttaccagct ttatcagctt
     1081 cttctttcac acgtccaagg tgagagaaaa gaattgcacg tccaccttgt tcgatgatgt
     1141 acttaatagt tggaagagct gctgtgatac ggttatcgtt agtgattacg ccatctttca
     1201 atggtacgtt gaagtcaaca cgaacgagga cttttttacc tttcaagtca acgtctttaa
     1261 cagtaagttt tgccatgtta caaaaactcc tttatttatt ttattcgaaa ctattatatc
     1321 acactttgga aatataagga aagatttcac aagatttttc gatatttaat ctttatcttc
     1381 tcttttccgt ctcaaggtca aggctaaact agcaagaccg aggctggcta agcctgctat
     1441 tagggcttca ttagcatcag ctgttcctgt atttggcaat tgtttgcttg cttcttctga
     1501 tttaactgct gtattttgag ctggtttttc ctgtgtagct actactgctg gtactgtttt
     1561 tacttttgtt ccaatttcaa caatttctgg aatcgcttct tggatgactt ctgttgaacg
     1621 aagacgacgc tgaccgtttt catcaacttc aaagacatgt cttaacagtc catctcgtcc
     1681 ctgtatgatg actcgttgtt ccccaactag catctcagca ttttcatgct cttgacgatg
     1741 aaaggcaacc ttttcctctt gaacttccaa tttaggagcc ttttgttgat cagaagtcgg
     1801 ttgataagta ggttttggaa ggactttgtt ttgtggtgct ggtttagcag gttgtggttt
     1861 ttcagcagat tcataaacaa aggcataatg agtaaagtgt ggtgctgtaa agataacatg
     1921 actatccgtt tgttcaaaag tcaattctac tgcctctttg ccttcaggta agaaaaatac
     1981 tttcttaact ttcttatctt tttcaattgg gattttcaca atagaagcgt aagagagatc
     2041 aacatcttga cctttttcat ccaaaccttc tatttcaaag acatgagcat cttctccagc
     2101 aaagtatttc ttctcttcag cacttgcttg aacacgctct actttcaaac ccttgatgac
     2161 agtcttctct ttatttgaga agtgtacttc aacacctgtt ttactatcag aaaagactgc
     2221 tggcttgtct tctttcaata aagcaaccag atcttttaga ctctgtttcg ctgttgtaag
     2281 ctcttctgga ctcaaatcta ctttttcaac caaggccttg tttttctcaa gaagcggagc
     2341 tagggcagaa cctgaaggat ggtttggttt ggcagccaag agttcttgaa cctcacctgt
     2401 taagcgatca agctctgtct tttcttccgt aaatttctct tgaccattca gagctttatg
     2461 agcttctgtc aatttattaa gtcggtcatt gacttgatct tgggtgctag tttcatggtc
     2521 tttcaacact tgttcggccg cttcaattgc tttcaagaag gcttctttta cttctgatga
     2581 agcaaagtta tagaccacct tgttcttagc agcttcaact tctgctaatt tttgtttgag
     2641 gtactcatcg ttcaatgctg cttttggaga taccaaaaca tcaaagctaa cttcatggcc
     2701 ttggtaatga agcgtcaagg tttgacgacc agtcttttga gcatcgtaac cagaaatttc
     2761 aactccctca tcagtaaagg aatgttcttc catggtgtca ttgctataag ccactgcaaa
     2821 gcgtccttca gacaagtcta agctatcacc aactaggtaa tcttttttcg gttcctgact
     2881 tacttcaatt cccaaaatag ttttcggact tgcttcgtct tggccagtga cagtcactga
     2941 caaattagca tttaccggtt gtcccaaata ttggagagta agattctgtt ctcccttatg
     3001 atgcgtatca aaacctgata ctgatacacc tgcgtgagtt aggcgaatga gttcgtcctc
     3061 agttcctcct tcatactgaa ctcgaagaac accacctcta aggtccaaat cctccccttc
     3121 ggcgtagctt gttttcttag gtcctgtttc taggctgact gacttgattc ttctgtcatc
     3181 ttttggaacg gaaactgcta ggacattact aatttccttg cctggcaact gggtacgata
     3241 ataaagaaga ccagattgat ttgtcaaatc caatggtgca cttgctaggt ctcctccaac
     3301 ttcgctcttc aaggttgcga gcggagtttg agaatttgga ttatcataaa cggtaatagt
     3361 tgctccagcc ggtacatctg caaagccaac ttgtaccttg ttattgccta gagaacgggc
     3421 tgcagccttg gccatcggaa tattgacact ctcagtatca agcttttcat acattttcca
     3481 gttatagata cgaatagcct tccatggagt tccattgtca gaagtgacaa cattcaagcg
     3541 ccagtcttga gcagtgattg gtttatcaag agtgatatct gtcacgtgtg ctttgttacc
     3601 acggacttcc ttagctagct tccactcacc atctgcatct ttataataaa ggtcaaagtc
     3661 tttagtgttc atcaagccat cgttaacaga ctcaccacca gctcctgcat gatccatgac
     3721 ccatctaaca acggtacgtg gcttggtcaa acgaatatcc acactaccac tcaactgagc
     3781 tgaagaccat ttatctgaca agctagtaat ggtaccgttc aacatacctt caataccttc
     3841 tccaccttca gtcttaggga aagtactatc aataactgtt gcacctggaa cgatattttc
     3901 agctagtggt tttggtaggc tggtatcttt tacagtcata ccccaatcaa aggttgtggt
     3961 tgcagcttct gaacgaactc catttttacc gactgctaca accttcagtt cttgagttgt
     4021 accctgagca cttgctgagc ggctaacttt tggtagataa atagttgtag aagatgagcc
     4081 agttagtaat ttccagctgt ctccatcttt ttcataaact tcatagaaat ctgcatcctt
     4141 gttgccttta aattgcacaa ctgcttccgc ttcttgggca tttttaagag attgtttcac
     4201 tacagaaaag cttgtcggcg attgtggctc ttggtgattg tccgagatag ttaattgtcc
     4261 taggttaaac tgataatctt ttacagcacc ttcatgctcg aaaaatagtt tgactgcata
     4321 gatggtttta cccgctagtg agctaagatc aaattcttca tttgtccagt tgtcagaaag
     4381 ggttagctct ttccatgcat ctgcatcatc gaatttgtag tctggagttg tagagaatgc
     4441 catataaact ttagaacctt ttcctccctt gtgggcaaca cgaagtttgg tcttctcagt
     4501 tacttctaac ttagtagaat aaagtctcac atcctgatct gtcttaccag ctacatcacc
     4561 agagaattta agggaatttc cgccattata ggcatctgta aaatcatatt ctgcacgaag
     4621 tttttcccct gttgaagtct gccaccagcg ccatgttgga agaacacctg atactgaacg
     4681 ataattccac tcagaatcct tagaaacctt accatctacg aaccattttt taccatgacc
     4741 tgtattaaaa gaagtagtaa aagtattacc taccgctggc gtacggtccg caactaggtt
     4801 agcaatacca taccaatctt tgtcacctgg tttttggcca gtggggtctc cttgataacc
     4861 tgtaaagaag atatcttcat ttttatgata atcttcacca gtttttccta aacttgtaat
     4921 ggtatctggg gcaaataaac caagagaaag gcgcaatttc ccattttcgt ctaaaatgtc
     4981 attccactta acctttgtct tgtaggaacc accctgttgc aattccaaac ctgcaaatac
     5041 atcataagga ttacgaccaa tccagttggc agttgcaata gtgtaatcat ttttagcctt
     5101 atcccagtta aagttagcaa agaagttatc tgccggaacc ttatctcctt ctggttgcat
     5161 gaattggtag ttgtattctc ccaaaccatc ttgatggtaa cgtccatagt tataggtcat
     5221 ggcatcatac caagaatact tgattggatg gtttacctta gcagcatatt ccttgctata
     5281 gagcataaac tggcgcatct tttctccaag aggtttaacc aaatctccag ttgtttcttg
     5341 gttgatgaaa tagccatcat agccataata ctttgccatg tctaccaatt tacgggcaat
     5401 tgggaagcta ccatctgcgt cttgcttcaa agcttcagca aatctttctt gatctgcaat
     5461 actattagac cagttgaaga agagtgtacc gtatacagga accccgttac ggtgacctgc
     5521 atcaataacg tcaggagttg gtacgagacc ttcccagaag accattgaat ctagatattg
     5581 ccaatagtca aaagcatagg ccttgaactc ttctccacca acagaagcat ggtcttttgc
     5641 tttagaattg gtgtttgata aggcttgaac ttttgcttcc ttgctagctt tttcattgac
     5701 taaatgacct gtccgacgtg aagcgaggac aacagatccg cggttaatag catcgtcttc
     5761 acgagcacct ggttcccatt ttaacaattc ttcccaagaa ttaaatttga tttcctttgg
     5821 ttttaatgtt ttttctgtat ttttagggac atctggctgg acttcttttt cagtcttatt
     5881 agcttctgct gctggtttag actcatcccc ttggttagtt gcttcgggct ttgcttctgt
     5941 ttccttgtca gacttaggtt ttgcttctgc agatggtgtt tctgcaacca cctcacttgc
     6001 tttttcctct ggtttagctg cgctagctgc ttctgcagtt tcagtagttg cagcttcatt
     6061 gcttgctggc gaagcagttt cagatgctat cgctactcct tctgcctttt ctagcttgtt
     6121 ttctctagca tgttcccctt cattctgctt atcagcttca ttggtcgaat gctcactctc
     6181 tcctgaaaca agctctgtat tagctccgct attttcagga actgcagttt cttcagccaa
     6241 ggctggacca gcaaataaaa cagcaccaat catcagcgag caggctccta ctgataactt
     6301 acgaatactg taacgacaac gtctttcaaa aaatggattc ttcattttct cctcctaaaa
     6361 gattgatttt gttgttttca agaaagcgct ttatattctg tagtttttta tcaaactcta
     6421 gaaccatgat aatcacattt ttatcctaaa acatttttag acaaaaaaca atagctgctt
     6481 tcataaatct a
//
LOCUS       DAVLYR010000102         6404 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLYR010000102 DAVLYR010000000
VERSION     DAVLYR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.102"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(17..226)
                     /locus_tag="VYO63_001517"
                     /pseudo
     CDS             complement(17..226)
                     /locus_tag="VYO63_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            436..2205
                     /locus_tag="VYO63_001518"
     CDS             436..2205
                     /locus_tag="VYO63_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV1672414.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPIANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFFVA
                     ALILHADKSTEDSLEAAQEATQAAKAQSKGQLVSTSVDAVISTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            2229..4184
                     /locus_tag="VYO63_001519"
     CDS             2229..4184
                     /locus_tag="VYO63_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1672415.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            4186..4623
                     /locus_tag="VYO63_001520"
     CDS             4186..4623
                     /locus_tag="VYO63_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1672416.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4685..5821
                     /locus_tag="VYO63_001521"
     CDS             4685..5821
                     /locus_tag="VYO63_001521"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV1672417.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1965 a         1077 c         1286 g         2076 t
ORIGIN      
        1 tccagccttc atgtcagact ttatgaatca tttcttgttt taaatcagga gaatagtaac
       61 gattttttcc ttttttgacg aactctattc cgtaacgatc aatcaattta atcatgtacc
      121 taagattaga attatttatc ccaaatttat ttgaaagctt ctctaagata tatccttgtt
      181 ttctaagttc atagatctga actttatcat cataagttaa tttcataata aaaacacccc
      241 aaaaagttag attttttctg tctaactttt ggggtgtagt tcatgtacac cggatatgat
      301 gcgttttatc atttttaagc ctttttgccc agcctcgtca aaagtaatgt tttgacacaa
      361 aatttgtgac aaaactttag ttttaaaggt ttttaacttt gtatatacta gttttaagaa
      421 aaggaggatg atctaatgga agaaaaagta tcattgaaag tcagggttca aaaactaggg
      481 acatcgcttt caaatatggt tatgcccaat attggagcat ttattgcttg gggagtattg
      541 actgccctct ttatcgctga tggctatctg ccaaatgaac agttagctac tgttgttggt
      601 cctatgttaa cgtatttatt gccaatcctg attggttaca caggtggata tatgatccat
      661 ggccaacgtg gtgccgttgt aggagctatt gctactgttg gtgcaatcac aggttctagt
      721 gttcctatgt ttatcggagc tatggtaatg ggcccactgg gaggatggac tatcaagaaa
      781 tttgatgaga agttccagga aaaaattcgt cccggatttg aaatgttagt taataacttc
      841 tcagctggtc tcgttggttt tgcattattg cttttggctt tctacgcaat cggtccagtc
      901 gtatcgactc ttactggagc tgttgggaat ggtgttgagg ctattgtcaa tgctcgcctc
      961 cttcctatag ctaatattat catcgaaccg gctaaagtcc ttttcctcaa taatgccctc
     1021 aatcatggca tttttactcc tctgggagta gaacaggtag ctcaagctgg taagtcaatt
     1081 ctcttcctat tggaagctaa tcctggacca ggcctgggaa ttctattagc ttatgctgta
     1141 ttcggtaaag gttctgctaa atcttcttct tggggggcaa tggttattca tttcttcgga
     1201 gggattcatg aaatttactt tccttatgtt atgatgaagc ctactctatt tttagctgct
     1261 atggcaggag gtatctctgg aacttttact tttcaactct tagacgctgg tcttaaatct
     1321 ccagcttcac caggttctat tattgcgatt atggctacgg cgccaaaagg tgtttggccc
     1381 catctaaatg ttcttctagg tgttttagtg gcagcagttg tttctttctt tgtagcagcc
     1441 cttattcttc atgcagacaa gtcaactgag gattcgctcg aagctgctca agaggctacc
     1501 caagcagcta aagctcagtc taaaggtcag ttagtatcaa cttctgttga tgcagttatt
     1561 tcgacagact cagtggaaaa aatcattttc gcctgcgatg ctggtatggg aagctctgct
     1621 atgggagcta gtattcttcg agataaggtt aaaaaagcag gtctagagat tccagtatct
     1681 aatcaggcaa tctcaaattt gcttgataca ccaaaaacat taattgttac tcaggaagaa
     1741 ctgacaccaa gagctaaaga caagagtcca agtgctattc atgtttctgt tgataatttc
     1801 ttagcgtccc ctcgttatga tgaaattgta gcttcattaa caggagcttt tccaatagca
     1861 gaaattgaag gagatatacc aacttcagca ccagtagata gtcaggaaat tgaccttaac
     1921 catattgatg ctgtagtagt tgcttatggt aaagcacagg gaactgcaac tatgggctgt
     1981 gaaacgattc gggctatctt tagaaacaag aatattcgta ttccagtttc tactgccaaa
     2041 atttcagaat taggtgaatt taattctaaa aacataatga ttgtaacaac tatttcttta
     2101 caggcagaag tgcagcaagc agcaccgaat tctcaatttc ttattgtgga tagtttagta
     2161 acaacaccag aatatgacaa aatggctgct agaatgtaca aatagaacta gaggtttcta
     2221 aattacgaat gctattaacc aaacgagaag aacaattatt gaaggctttc ctacatgtag
     2281 ggaagctttc aatgcaagat atgactgaaa tcttacaggt ttcatctaga acaatttatc
     2341 gaactttatc agatttgaca gatagcatgg agcaatatgg aatcgaaata acgaagcatg
     2401 ggaaatacta tattttgact ggagagttgg atgatttgcc gacagaactt gaagtgttag
     2461 ttgagtatag tccccaagaa agacaagagt tgattaccta tcgccttctg actgagagtg
     2521 gttttgtcac caatgaagca ttgcaagagt gcacgaaagt cagtaatgta actattattc
     2581 aggatatttc agatattgat aagcgtcttt tagactttga tctgaaaatt gaacgacaaa
     2641 aaggttatcg gatttctggt gattcagttg gtaagagaag atttttggct attttactga
     2701 caaactgtat ctcagtagca gatttttcaa ccggtaattt tgggagcttt gatattttag
     2761 aagcagatag aactaggctg gccagtcaga ttgttaataa gcaactgtca ggttttccag
     2821 atatggatgc taggatgaag atgttttttg cgatcttgtt atctcttata ggtcaggagc
     2881 aaaacattga aaattcacct aatactagta agcaggcttt ggaaatttct caaaaaattt
     2941 ttcaagctta ctctaagcag actgcacaat tttatagtat tcaggaaatt atctattttg
     3001 cgagcatctt ggatgaatta atcattaaac gtcaggacaa tccgctcttt acggagaaat
     3061 ttgatggtga atttttctac aatatttcaa atctgattga tacggtttcc atgtatacca
     3121 agattgactt ttttaaggac aaggttttat tcaattttct tttccatcat attcggctca
     3181 gtttaggcgt ccctatcctt tttcagggtg aaaatttgcc agaatctgtc cagattttag
     3241 ttgaaaggaa taaatttctt tatacagtca tcagtctttt agtgaatgat atttttccga
     3301 aatatcttca tacagagtat gagtatggca tgattgccct acattttatc tctagcttag
     3361 gccgtagtcc agagatttat ccagtccgtg ttttgctttt aacggatgaa cgtcgggtca
     3421 ctagagattt attagtcagt aaaattaaga gtgttgctcc ttttgtagag ttgatagata
     3481 ttcagtctct agtagattac cacagtattg atctcagtca gtatgattat attttatcta
     3541 ccaagccgct gactaatcag gaaatcgatg taatttctag ttttccaacc gtcaaagaat
     3601 tgcttgaatt acaggaacga cttcagtatg tacaggcaca tcgtacaatt gtcgcgcgtg
     3661 atgctatcgc tccagagaaa agttatgact tgcaagatta tttaatatct agtagtcagc
     3721 ttttgagtca attcgagttg gttcaattgg agaataatca atcatttgag cacacggtag
     3781 aacaaatcat ccaatatcag aagaatgtga gtgacagaga ttacctaaca agaaaattgt
     3841 tatctcactt ccagaatagt cctatggcta ttcctaatac tggtctggtg cttttacata
     3901 gtcagtctag caaagtaaca acaaatagtt ttactatgtt tgaactcaaa ctacctatct
     3961 ccgcattgtc aatgaaacga gaggaagaag aggtcaaaag gtgtctgcta atgctaatgt
     4021 ctaaagaagc tagcgaggaa gcgagagatt taatgacagc tattagtcag tcgattattg
     4081 aaaatcatct ttatacagag atttacaaga cgggaaatca atccattatt tatcagatgc
     4141 taaatactat ttttaacgaa aaaattaaga aattggagaa ctaatatgaa acttgaaaaa
     4201 catttgatta agcttaataa acaattttct aacaaggagg aagctatttg ttattgtggg
     4261 caagttcttt atgagggtgg atatgttaat gaagactata ttgaagccat gattgagcga
     4321 gataaagagc tatctgttta catgggtaac tttatcgcaa taccgcatgg aacagatgca
     4381 gcaaaaaagg atgtcctcaa gtctggtatt acagtcgttc aagtccctag aggggttgat
     4441 tttgggaatg tatctaaccc tcaagtggca acggttcttt ttggtattgc tggtattggt
     4501 aatgaacact tagaaattat tcagaaaatt tctatcttct gtgcagatgt agataatgtt
     4561 cttaaattag ccgatgctca gtcaaaagag gaagtattgc gcttatttga tgctgttgaa
     4621 taattgaatt tcgtcatttg ccatctagta tatatgtccc tcaaatagga aaaggagaaa
     4681 ttgaatgaaa cattctgttc attttggtgc cggtaatatc ggtcgtggtt ttataggtga
     4741 aattctattt aaaaatggtt tccatattga ttttgtggat gtcaataatc agataattca
     4801 tgctctaaat gaaaagggca agtatgaaat tgaaattgca cagaaaggac agtctcgtat
     4861 agaagtaact aatgtggctg gaattaatag caaagaacat cctgagcaag tcattgaagc
     4921 gattcaaaag acggatatta ttactactgc aatcggacct aatatactcc cttttatcgc
     4981 tgaacttcta gccaaaggaa tcgaagctcg ccgagttgca gggaatacac aggtattgga
     5041 tgttatggcc tgtgaaaata tgattggcgg gtctcaattt ctttatcaag aagtcaagaa
     5101 atatttaagt ccggaaggtt tgacatttgc tgataactac ataggttttc caaatgctgc
     5161 agtagacagg attgttccag cacaaagtca cgaagattcc ctttttgttg tggtcgagcc
     5221 ctttaatgaa tgggtcgtgg aaaccaagcg tcttaaaaat ccagatttac gtctaaaaga
     5281 tgtgcattat gaagaagatt tagaaccctt tattgagcga aaactttttt cagtcaattc
     5341 tggacatgca acttcagctt acattggtgc gcattatggt gccaagacaa ttttggaagc
     5401 tcttcaaaat cctaatatta aatctcggat tgaatctgta ttagctgaaa ttcggagtct
     5461 cttgattgcc aaatggaact ttgataaaaa agaattggag aattatcaca aagtcattat
     5521 agaacggttt gaaaaccctt tcatagtgga cgaggttagt cgcgtagctc gtactccaat
     5581 ccgaaaatta ggctataatg aacgattcat ccggccgata cgtgaattga aagaactcag
     5641 tttgtcatat aaaaacctac ttaaaacagt tggctatgcc tttgactatc gcgatgtaaa
     5701 tgatgaagaa agtattcgat taggtgaatt gttggctaaa caatcagtca aagatgttgt
     5761 tatacaagtt acaggtttag acgaccaaga attgattgag caaattgtag agtatattta
     5821 atatttttcg aaaatctctt caaatcaggt tagcatcgct ttgtcttagg catatgttac
     5881 tgacttcgtc agttctatct acaacctcaa agcagtgctt tgagctgact ccgtcagtct
     5941 tatctgcaac ctcaaagcag tgctttgagc tgactccgtc agtcttatct gcaacctcaa
     6001 aacactgttt gagttatctg cggtaatctt tctagcttgt ctttgatttt tgttgttatt
     6061 tataaggtaa aagaagctgg acaaaaagtc ttcaaaatcg gaaaaaggca gcctatcggg
     6121 tgttcaaaaa tcttgatagg atgtccttta ttatggaaag ccttattgga ttttctcctc
     6181 agattgagtt tttgatcagc tttatgagat aggttttgct agagatgtag cccatcatgt
     6241 tatttttatg gacggtggga aaattgttga aaaaaataat gcccatcaat tctttagtcg
     6301 tccaagagaa gaacgaacca agcaattttg gaacgaattc tttcgaatgc gatctatata
     6361 gtaaaatgaa acaagaacag gacaaatcga tcaggacagt caaa
//
LOCUS       DAVLYR010000103         6312 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLYR010000103 DAVLYR010000000
VERSION     DAVLYR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.103"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..1133
                     /locus_tag="VYO63_001522"
                     /pseudo
     CDS             1..1133
                     /locus_tag="VYO63_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1321..2883)
                     /gene="guaA"
                     /locus_tag="VYO63_001523"
     CDS             complement(1321..2883)
                     /gene="guaA"
                     /locus_tag="VYO63_001523"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1672418.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3025..3723
                     /locus_tag="VYO63_001524"
     CDS             3025..3723
                     /locus_tag="VYO63_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1672419.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERHVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3766..4662)
                     /locus_tag="VYO63_001525"
     CDS             complement(3766..4662)
                     /locus_tag="VYO63_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1672420.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQTDIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4671..5606)
                     /locus_tag="VYO63_001526"
     CDS             complement(4671..5606)
                     /locus_tag="VYO63_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1672421.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5603..>6312)
                     /locus_tag="VYO63_001527"
     CDS             complement(5603..>6312)
                     /locus_tag="VYO63_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1672422.1"
                     /translation="VRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLGDQTGLE
                     KLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEI
                     FSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLES
                     SEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSEK
                     FFLCKDRNNVELWFEEV"
BASE COUNT         1951 a         1378 c         1149 g         1834 t
ORIGIN      
        1 tctaaaattc cttatcttga aacgactggt atgccttcta gaattctcct tagaaaacgc
       61 cgtttcaagt gctatcactg ttcaaaaatg atggttgctg agactcccct ggtaaagaag
      121 aatcaccaaa tccctcgtat catcaaccaa aaaattgctc aaaagttaat tgaaaagatt
      181 tctatgactg atattgccca tcagctttcc atctcaactt caactgttat tcgtaagctc
      241 aatgattttc actttaaaca tgatttttcg cgtcttcctg agattatgtc ttgggatgag
      301 tatgctttta caaagggaaa gatgagcttc attgcgcaag attttgaaaa gctcgatatc
      361 atcactgttc ttgagggtag aacacaagct atcattcgaa atcactttct tcgctacgat
      421 agagttgtcc gatgtcgcgt caaaattatt actatggata tgtttagccc ttactatgac
      481 ttagctagac agcttcgctt tcaaatttct aggctcaggc tgaaacagtc tcccagactg
      541 tttcactccc gaatgctaaa atcgttcttg atcgctttca cattgtgcaa catcttagcc
      601 gtgctatgag tcgtgtgcat gttcaaatca tgaatcagtt tcatcgaaaa tcccatgaat
      661 acaaggctat caagcgctac tggaagctca tacaacagga tagccgtaaa ctgagtgata
      721 agcgttttta tcgtcctact tttcgtatgc atttaaccaa taaagagatt ttagacaagc
      781 ttttgagcta ttcggaagac ttgaaacacc actatcagct ctatcagctc ttgctttttc
      841 acttccagaa taaggaacca gagaaatttt tcggacttat tgaggacaat ctgaagcagg
      901 ttcatcctat ttttcagact gtctttaaaa ccttcctcaa agataaagaa aagattatca
      961 acgcccttca actaccctat tcaaacgcca aattggaagc gaccaataat ctcatcaaac
     1021 ttatcaagca caatgccttt ggttttcgga actttgaaaa cttcaaaaaa cggattttta
     1081 tcgctctgaa tatcaaaaaa gaaaggacga aatttgtcct ttctcgatct tagcttttct
     1141 tcaacccact acagttgaca aagagcctga aaactacggt ctaatttttt gtccaaatgt
     1201 aaaccacttg gaactattat tcaatttatt ctttttagca aatgttcccg ttttaatccc
     1261 agttacagtt tttaatcctt tgtaaccgtt gattttacaa agcttttaat ttttttgcga
     1321 ttactcccac tcaactgttg caggtggttt acttgtaata tcgtagacga tacggttaac
     1381 atgatccact tcatttacga tacgtactga gattttttga agtacttccc atggaatttt
     1441 ggcaaaatca gcagtcatac catcgataga agtgatagca cggattgcaa tcgtgtagtc
     1501 atacgtacga ccgtcaccca taacaccgac tgaacgaacg cctgtgttaa cagtgaagta
     1561 ttgccaaata tcgcggtcaa gtccagcttt agcgatttct tcacgaagaa tagcgtctga
     1621 ttcacgaacg gtttcaagtt tctcttcagt gatttcaccc atgacacgga tagcaagtcc
     1681 tggtcctggg aatggttggc gccatacgat atggtctggc ataccaagct ctgtaccaag
     1741 agcacgaact tcatccttgt aaagagtatt gagtggttca atcaattcaa actgcatatc
     1801 ttctggaaga ccacccacgt tgtggtgtga cttgatagtt tgagctgtat ccgtaccaga
     1861 ctcgataaca tctgtatata aagtaccttg agcaaggaat ttcacatctt tgagcttgct
     1921 tgcttcgtca tcgaatacat agacaaactc gttaccgatg attttacgtt tttgttcagg
     1981 gtcagaaacg ccagcaagtt tgtcaaggaa acgtttagca gcgtctgctt tgacgatatt
     2041 caaaccaaac ttaccaccga gcatgtccat aacttgatca gcttcgcctt tacgaagaag
     2101 accgtggtcc acgaagatac agatcaattg atcgccaatc gctttttgga gaagaacccc
     2161 aacgacagat gagtcaacac cacctgatag accaagaagg acacgtttat caccgacggt
     2221 ttcacgaatt tttttgatct gcatgtcaat gaaattatcc attgaccagt cacctttagc
     2281 cttacaaatg ttaagggcaa agttacgaag gatatcattt ccgtatacag aatgacgaac
     2341 ttctgggtgg aattggatac cgtaaatgtg tttatctggg ttttcgatgg ctgcgtatgg
     2401 gcagtcagct gatgtacctg tacgaacaaa gtcagcagga atctcagtaa ccgcatcacc
     2461 atggctcatc aaaacagtct gttcatcagg tgttgattca aaaagcgctg atggtgtgtg
     2521 agttagggtt gattgaccgt attcacgatt tccagcatca cctgcaggaa caacttttcc
     2581 tccaagttta tgggtcaata actgcatacc ataacagatt cccaaaattg gaattccgag
     2641 ttcgaagatt tctgggtcaa tatcaaatga accatcttca tatacagaat ttggaccacc
     2701 tgatagaata attcctacag gattgacttc acgaacttca gcagctgaaa ttttatggct
     2761 ttttagttct gaaaaaacac caatctcacg gatacggcgt gaaatcagct ggttgtactg
     2821 gctaccatag tccaatacga tgattttttc tacatcttgc aaatcagttg aaatgttgct
     2881 catctttttc ctttctcaaa agaaatatct tttctcaata ttctatcaca aataagggcg
     2941 aattaccaac taaacaaggc gaagtttgac tttttctagt ttattggggt acaatagaga
     3001 aaagatagaa atgaagtgaa aaatatgcta cctgcttata tgaaaatcca tgatcagata
     3061 aaaaaggata ttgacgagca ccgttgggct attggtgaga ggcttcccag tgaaagagat
     3121 ttagctgagc agtttgcggt cagtcgcatg accctccgcc aagccgtatc tctattagtc
     3181 gaagaaggcg tcttagagcg ccatgtagga agcggcacct ttgtttccag tactcgagta
     3241 caagaaaaga tgcgagggac aaccagtttt actgaaattg tcaaatccca aggtaaagtt
     3301 ccctctagcc agctcatttc ctacaaaaaa accattccca atgagcaaga agttgccaag
     3361 ctaggaattt ttccaacgga caatattatc cgaatggagc gggtccgcta tgccgaccaa
     3421 gttcccctag tttatgaagt tgcttctatt cctgaaaaat tcattaagga ctttaaaaaa
     3481 gaagaaatca ccagtcattt cttccaaacc ttgcaaaaac atggctatcg tatcggcaaa
     3541 tctcaacaga ccatctatgc tcgccttgct aaagaaaaga ttgcccacta tttggaagtt
     3601 gaaaaaggac atgctattct tggattgacc caggtttcct acctagaaga tggtactgct
     3661 tttgaatacg taaaaagtca atatgtaggc gaacgctttg aattttatct tgaaaataat
     3721 tagattacca cgcaaaaact ctctgtcacg gacaaagagt cttttttatt tttctaagaa
     3781 taaatctttc aaagaaatca aagttacagc aaacaatatc attgccatac caagaaaatc
     3841 aatgggataa aattgttcat tcattattaa gaaggcaaag aaaatcgccg atattggctc
     3901 aattgaagcc aacaagcttg acttgaccgg tcctatcaga ctggctcctt taaggaaagc
     3961 tgtataggca aagacagtcc cgataaggat aatgcctgca aacgcaagga gaaaatcaag
     4021 actagtcggg atatcggtct gtagaacccc tgtaaaagga agggcgacca aacctgctat
     4081 gaccattccc acaccaatga ccaagctgct cccccacttt ttaatcaagg ctatgggtaa
     4141 aatgatatac agagcataag tcaaggcaga aaagagaccc cagaacagac cagcaggtgt
     4201 catggataac tggtccaact gcccatgtgt tgcgatcagg aaggttcctc cgatggcgaa
     4261 tatgatggaa actatctctc ccagtgtcgg tgccacccta tccttgatac agctataaat
     4321 taaaattccg acaggacaaa catactgaag caccgtcgct gttcccgcat tggtctcctg
     4381 aatagcagac agataggcga attggttgag aaaaagacca atcagagcaa aaataagaag
     4441 agacagcaaa ctctttctat cctttaaaaa gaccagtatt ttatcctttg cagtagcata
     4501 agccaagagc atgagaattc caccagcgat taaaagacgc aagttggtca agaccagagc
     4561 cgaaattccg tgtgccatta ggtattggcc actcgttcct gacaaccccc aagcaatacc
     4621 agccacaact gttagtaaag tcccttttaa actatttgac atgtctctcc ttactcttct
     4681 ttgcgaatca agttcatcac ttgattgcgg tctaatatcc attgagctct ctcgtccttt
     4741 tcataggtac gattcgatag gaaaatagtg gcttcttgct tctgacgatt ccacatgata
     4801 aaggtacctg tatagcccgt atggtctagc caatctcctt ccaaattcca tgctaaagaa
     4861 cgttccttgt catccaaagg agaaaaattt tgacttaagt ctcttgcaaa atcatctgct
     4921 aaatagtgtt ctaaaaagat ttgtaaatcc tttatagtcg aaaataaacc agcgctccca
     4981 gcatgtctac ccaggagacg agccttggga tcatgcacta tgcctgcctc tacacctcta
     5041 actgttggaa cagcaagctc aactggccca aacttggttt ccgtcattcc ccaaggtttc
     5101 cagacttgat cctttaaaat cacatccaaa tcttgattaa aaattctttc caaaataaag
     5161 cccaacagca aaaaatggac atccgaataa agaaaggctg gctgacttcg tctgttgaga
     5221 tgaaacatcg cttcctttaa ttcaggggct gttaaaagat cacgattagg aataaaagga
     5281 tcaaggtctg ttgcatgagt caagagctgg cgaatagtga tgtctggata atcactctca
     5341 ggtaaaaaat ctattaccag tctatcaata tctaattgac ctatttccca caagaaggta
     5401 caaactgtgc caaccccaac aaccttgctg acactagcta ggtcataaac tagtcctgcc
     5461 tcagtctgca agccatgctc tgggtcactc tggcctaaat agaactctgt ccattgattg
     5521 tccttaaaat acgcaaaaga ggctccggga taaattcctg cctcgatttg ttcttctatt
     5581 tttttaataa tcttggtcca cttcatactt cttcaaacca caattcaaca ttatttctat
     5641 ctttacaaag gaagaatttt tcagacttag gaataaaata ttcagtagac tcaaacttct
     5701 ggcgaagact tgctatgtct aattcattga ccaagaactt gagcatagat aagtcccagg
     5761 taaccgtatt gtccacagtc aaatcctgcc cctgagctgg aataaaatca agggatgccc
     5821 caatttcaga tgattccaag aaactttcga tatcagttgg gagatgtaat tccatagaaa
     5881 tctcaaattt acttaaagaa attgatgcca aatctgtttg aaattcaggc ttttctccta
     5941 cttctactag acttgctctg tcatcttccg catgaatcaa aatcaaatca tcttctggtg
     6001 agaaaatttc aaaagcgtag ccattttgac ctttatataa tcgatgaatc gaatctgttt
     6061 tagataagat tccttcaatt tctaagggat tttccacctt gacaatcaat ctagctagtt
     6121 tttttcttcc ctctacctta cgagtacgca tactgggagc ttcttctaaa accagctttt
     6181 caagacccgt ttggtcacct agtgacagaa aggccgattc ttctaacaag gccttcattc
     6241 caagggtttc aatataaaat gtttcattta attttctatt attagctttt aaagtaggaa
     6301 taatccgtac aa
//
LOCUS       DAVLYR010000104         6263 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLYR010000104 DAVLYR010000000
VERSION     DAVLYR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.104"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            77..241
                     /locus_tag="VYO63_001528"
     CDS             77..241
                     /locus_tag="VYO63_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1672423.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            325..891
                     /gene="amaP"
                     /locus_tag="VYO63_001529"
     CDS             325..891
                     /gene="amaP"
                     /locus_tag="VYO63_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1672424.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            903..1073
                     /locus_tag="VYO63_001530"
     CDS             903..1073
                     /locus_tag="VYO63_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1672425.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1112..1720
                     /locus_tag="VYO63_001531"
     CDS             1112..1720
                     /locus_tag="VYO63_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1672426.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1751..1954
                     /locus_tag="VYO63_001532"
     CDS             1751..1954
                     /locus_tag="VYO63_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1672427.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2049..2327)
                     /locus_tag="VYO63_001533"
                     /pseudo
     CDS             complement(2049..2327)
                     /locus_tag="VYO63_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2525..2812
                     /locus_tag="VYO63_001534"
     CDS             2525..2812
                     /locus_tag="VYO63_001534"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1672428.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3090..3860)
                     /locus_tag="VYO63_001535"
     CDS             complement(3090..3860)
                     /locus_tag="VYO63_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1672429.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3875..4723)
                     /locus_tag="VYO63_001536"
     CDS             complement(3875..4723)
                     /locus_tag="VYO63_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1672430.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4720..6195)
                     /locus_tag="VYO63_001537"
     CDS             complement(4720..6195)
                     /locus_tag="VYO63_001537"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1672431.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
BASE COUNT         2154 a         1185 c         1031 g         1893 t
ORIGIN      
        1 aggcagtgtt gtgatcaatt attgctggag gtcttattgg tctcttggca ggtaaaatca
       61 ctaaaaaagt agttctatgg gaatcatcgc aaatgtattc gctggtttag tcggggcata
      121 tgcaggacaa tctcttttag gtagttgggg tccatcaatc gctggaatgg ctttgctccc
      181 atctattgta ggtgcagcga ttgtgattac tgtagtgtca ttctttacag gtagaaagta
      241 aacttttcgc cagtaaagtt agcaaactat ttttaaatca atgacgggaa aaatagttta
      301 aatgttaaat cgaaaggatt gtatatgtca aaagcaaaga aaatatgttt cattattttc
      361 tgtattttaa tcttgacaat tttccttcct gttttgatag attatcatca agttagtgat
      421 ctaggtattc atctacttag ctggagacag aactccgtag ttgaattcta tcttgctaga
      481 tatgtctttt gggggacagt ggttctatca actttagttt tattatccat tttagttgtg
      541 atgttttatc ctaaacgtta cttggaaatc caacttgaaa ctaaaaacga tacattaaaa
      601 ttaaagaatt cggcaatcga aggttttgtt agaagtttgg tgagtgatca tagattgatc
      661 aagaacccaa ctgttcatgt aaatttacga aaaaataaat gtttcgttca tgtagaaggt
      721 aaaattcttc cttcagacaa catcgctgac agatgccaaa taattcaaaa tgaaataact
      781 aatggattga agcagttttt tggtattgag cgtcaagtaa aacttgaagt tgcagtaaaa
      841 aattaccaac caaaacctca aaacaaaaag actgttagtc gtgtgaagta aggaagtaaa
      901 aaatggaatg gcttaaacaa tatcgatatc caattatcgc tggtctcata ggcgtatttc
      961 tggcttgttt gattgtctcc tttggcttct tcaaaacaat atttgtattg attttaggag
     1021 cactgggagt tgcagctgga ttatatatcg aaaaaaacta tatagataaa taaaaaaata
     1081 aaaattacta atttaattaa aggagtttca tatgtcaaac gaaaaaaaca caaacactaa
     1141 cgtagaaaag aaagatgcta ctgttgtagc tcacgaaatc aaaggggaac ttacttacga
     1201 agataaagtt atccaaaaaa tcattggtct ttcactagaa aacgtttcag gtcttttggg
     1261 aatcgatggt ggtttcttct caaatcttaa agaaaaaatc gttaacagcg atgacgtaac
     1321 aagtggtgtt aacgtagaag ttggtaaaac acaagttgca gttgacttaa acgttattgt
     1381 tgagtaccaa aaaaatgttc cagctttata ttcagaaatc agagaaatcg tatcttcaga
     1441 agttgctaaa atgactgact tggaaattgt tgaaatcaac gtaaacgttg tcgacatcaa
     1501 aactaaagaa cagcatgaag cagactcagt aagccttcaa gatcgcgtat ctgacgttgc
     1561 tgaatcaaca ggagaattca cttcagaaca attcgaaaaa gctaaatctg gtcttggatc
     1621 tggtttctca actgttcaag aaaaagttag cgaaggtgta gaagctgtta aaggtgcagc
     1681 aaatggtgta gtatctcacg aaaacactcg tgtaaactaa gataaaataa atataacagg
     1741 agaaattatc atgtcagtag aagaaaaatt aaatcaagct aaaggttcta ttaaagaagg
     1801 tgttgggaaa gccatcggtg atgaaaaaat ggaaaaagaa ggagcagctg aaaaagttgt
     1861 ttctaaagta aaagaagttg ccgaagacgc taaagacgct gtagaaggtg ctgtagaagg
     1921 tgttaaaaac atgttgagtg gcgacgataa ataaggttaa aagttacttt atctttttgg
     1981 taacattagt caagatgatt ctcagaaaac aaaaagctag agattcccaa ttgcggaacc
     2041 ctagcttttt aattttgcct ctttctctta ttatatttca gcaggttgtt ggccatgagt
     2101 acgaatccca tgtcaattct cacttgacgc ttccctctca gatgacatct cttataaccc
     2161 aaacaaacct ttatctgccc aaagacagat ttcatatcaa tcttacgttt agcgaaaatt
     2221 tgtctaccct tggaagataa aagtgcctga tattctttag tttttaaaca ctggtaacgt
     2281 tcattcatat acagtccctt ttgaggggct gattcaggtt cataatcgca gtcaacattg
     2341 atttcaaggc tgtttgcttt ctatctcccc tttttcatga tatattttcg tacaggtgct
     2401 tcaatagttt ctatagtaaa atgaaccaaa aatagtacac gttgcggtat aatgttcttg
     2461 tggcatattc attagatttt cgtaaaaagt tctctcctat ttgtgagcga acaggtatag
     2521 tcaattgatt gtcagtttga agaccaagag tcgtaaggca aaggatatgg cagaatccat
     2581 ccaagggtgg ctggcacaat ttctagtgaa cctctttaaa tctattacgt ttgactgtgg
     2641 aaaagagttt tcaaaatgga aagatatttc caatcatcac gatagtgaga gcttttttgc
     2701 gaatctagga tgtccaagac agcggtgtct caacgaacat tcaaatagac ttttaaggtg
     2761 tcatgacttg ccaaagcaga cagatttcaa cgaagtcagt caagaatttt aggctgggta
     2821 aaaactcaat ttcaggagaa aatgaaataa atattcccac aataaaatgc atagtatcaa
     2881 agtttttcca acacctgata ttatgcattt tttaatttta aagacttttt cccagcctca
     2941 agaaatcaca tcccacctaa atcctttgga taccaaatac ctcatcaggt attttctgag
     3001 ttggtaagac tgtctagctt aatttgacaa atggagggcg ttaaatccag tttttacagg
     3061 aagtctattt aaatattttc acactgacct tacaaaatat gaaacaaatt atataaagtc
     3121 attcccatga caaagaataa catcccttta aaacagtatc ttacccaatt ttcaggtaag
     3181 acttttgata caaccgtccc caatacccct ccgcatatag aagcaagaaa aataactgga
     3241 acaggtgcta aatgatactg atttaaacct gtaaccacaa caattgtagc caaatgagac
     3301 agctgggaaa agaaaataat tgcaagagaa tacatcgttg cttcttttat cgaaatagaa
     3361 aaaaacacca tcaataaaga tacattgagc ggaccaccac caatccctaa aaaactagaa
     3421 attaagccca atagaagacc acaaatcata tagaatccta tcttatcaaa ttgataagtt
     3481 tttttaaaat ctgttaaaac aaatgataca agtagcatca caaatagtaa aatcatttga
     3541 acaatggata ctagccgtac tgataattga gataacaaaa cttgaaaaat aagatgacct
     3601 agaaatcctc ctaaaacaga agaaaaggat accgtaaaaa tcaatctcca ttttatctct
     3661 ttactttgtg aaaatctttt gattgttgaa ataatcgcca ttataaaaac agagaaacta
     3721 gaataaaaag caatatcact gaccgaatgg tatccaaaac tatccataag tggcttaatt
     3781 ataattccac cacccatgcc tgatatagaa cccaatccac tagctattat aatgattaga
     3841 aaataaataa tctcaaccac tttttaagtc catcctattc tttcaaacat ctaaaaccta
     3901 gatggctact agtggaagta gaaatacatc catttctagc agctactcgg tatcgattac
     3961 aataagaaca atgacataaa aaggaacctc ctctgatagc atactcctca tcttgtatcc
     4021 catattttgg caactcatag tcatcttcat taaaactagc taataaggta tatctcggat
     4081 tgcgacacca ttcccaaaca ttcccaatca tttgatatag accaaaatca ttaggtaaaa
     4141 attcatagac aggagccgtt cctataaatc catctaaggc agtattttca taaggaaatc
     4201 ttccttgcca agtattggca tgataatatc caccttctaa aagagtatct ccccaaggat
     4261 attcagattg tcttcctcct ctagcagcat attcccactg tgcttcagtc ggtaaagaca
     4321 ttccgctcca attacagaaa gccaatgcat cttctaaagc aacatgaact actggatggt
     4381 cttctaaacc cactagatta ctattctcac cgtagggatg tttccagcaa gcgttcggaa
     4441 cttgtaacca ccacggagcc ccagcaggat gaggatatcc ctccctttct gcctctggaa
     4501 cgaacaatat aaaaacaaaa gaccactctt gtcgctcagc tagtgtttta tagcctgttt
     4561 ctttcacaaa ttgagcaaat tcttgattcg taactggagt atccgcaatg gaaaaatcct
     4621 ttacactaac agcgacttga ggaccttcaa aatcatctaa aaagccctct tcagaatttg
     4681 ttcccatcat aaatgtccca cccttgattt ttttcatttt catgattgac tatcccccgt
     4741 tttcgttaga gttggcgtta ttttagaaag ttcaacagga actaactctt catccacaac
     4801 aaaaccttct tctctatatc taagaaagtc tactaatttt ttcttcatct gacgaacaat
     4861 aggttgatat ttttcacttg gatacaaatc attcatttca tgcggatctt ttttcatatc
     4921 aaataactga taatgattta aaacaggaaa ccagataaat ttccactggt ctgtcaaaat
     4981 atattgacta gaatcttttc ctaaagcatg ttcaccatga aattctgttc gccacccctc
     5041 gtactgtcca aataataaat tcttaacact ccgtccatct agttcatctg tagtagttcc
     5101 tcctgccaaa tccacgagtg aaggaaaaat atcttgaatt ttcactaatt gcttgattgt
     5161 tcccctatta ccagctatca atccagccgg atcgtaaata aacgaaggaa tgtggatact
     5221 tccctgataa ggatatccct ttctaaacag ataatgttca ccaagctgat ctccatgatc
     5281 cgaaacaaac cagataatcg tatctttatc atgacgaaat tcttttaggg cagtcaaaaa
     5341 gcgactaatt tgatggtcta tatgagtaat caatccaaaa taagcagcaa ccattcttct
     5401 ttgatcatct tcctttaatt tccctcttaa agcataaata gacggaattt gcttttctaa
     5461 tacttcccaa ttcccgatat gcaaatcgag aaattgaggc aagcgttcca tatacatatc
     5521 aaaatagtat tttgggggat ttaatggagc atgtggtttt tcaaaagaca ttttgagaaa
     5581 aaaaggaacc gttggatctc ttctttgtaa aaatgaaatg gactcactca caacccagtt
     5641 tgttggatgt aacttctcat ctttatccca aggtctagct tcccaagaat tacaatccat
     5701 tccgtcatca atcaaatcaa catcataacc aactttaccc tttaaaaagg ctagatagtc
     5761 actagcataa tcaaattgac ttccataagc cttatcatac tttctatcaa catgtagata
     5821 cccatcgtga agtaagacat gatcaaaccc aagtctctgt ctggaaggaa aaacatgcat
     5881 cttaccaata cactctgttt gataacccat atctttaaag acttttggaa gagtattagt
     5941 aaaattccaa ggcacctcat cttgataacc aactcgtcca cttttatctt ggtctaagcc
     6001 agtcaataaa gctgccctag caggaacgca agaaggaact ggagaatagg cattttcaaa
     6061 attataacct acactagcca tcatatctaa tgttggagtg ctaactagct tatccttact
     6121 attcaaggat aaggcgtctg ctctcatttg atctacaaca atcaaaataa tatttggctg
     6181 ttttgtctga accataaaat ctcctttcta atatggcaaa agaggcacaa gaagatatct
     6241 acctttactg cacccctttc tat
//
LOCUS       DAVLYR010000105         6172 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLYR010000105 DAVLYR010000000
VERSION     DAVLYR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.105"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..645
                     /locus_tag="VYO63_001538"
                     /pseudo
     CDS             1..645
                     /locus_tag="VYO63_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            756..1682
                     /locus_tag="VYO63_001539"
     CDS             756..1682
                     /locus_tag="VYO63_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1672432.1"
                     /translation="MDLSQTMPSYAQVVSLYDDTSISVSNNETDKVLAGSLYTDTNEQ
                     GLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQFQTKIVRVINDE
                     DDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQKNKKYTVLS
                     QQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIGVMKAVGYQ
                     NRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFELNLLSVLG
                     TLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1859..2866
                     /gene="gap"
                     /locus_tag="VYO63_001540"
     CDS             1859..2866
                     /gene="gap"
                     /locus_tag="VYO63_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1672433.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2933..3769)
                     /locus_tag="VYO63_001541"
     CDS             complement(2933..3769)
                     /locus_tag="VYO63_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1672434.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3895..6090
                     /locus_tag="VYO63_001542"
     CDS             3895..6090
                     /locus_tag="VYO63_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1672435.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
BASE COUNT         1893 a         1113 c         1330 g         1836 t
ORIGIN      
        1 atagacttaa aaactatctt actgacaatc cagacgctta tttgactgaa atagcttctg
       61 aatttggctg tcatccaact accatccact atgcactcaa agctatgggc tacactcgaa
      121 aaaaagaacc acacctacta tgaacaagac ccagaaaaag tagccttatt tcttaagaat
      181 tttaatagtt taaagcacct atcacctgtt tagattgatg aaacaggatt tgatacttat
      241 ttttatcgag aatatggtcg ctctttaaaa ggtcagttaa gaagaagata tcagaggatt
      301 tctttggttg caggtctaac aaatgaatgg tgagttaatc gctccaatga cttacgaaga
      361 gacgatgacg agcgactttt ttgaagcatg gtttcagaag tttctcttac caacattaac
      421 cacaccatcg gttattatta tggataatgc aagattccat agaatgggta agttagaact
      481 cttgtgtgaa gagtttgggc ataaactttt acctcttcct ccctactcac ctgagtacaa
      541 tctattgaga aaacatgggc tcatatcaaa aagcacctca aaaaggtatt accaagttgc
      601 aatacctttt acgaggcttt tttatcctgc tcttgtttca attgactata gttcacggat
      661 acagttggga aagaagctat taaatatttg gagagtcctt ttgggatgaa catgtcaaat
      721 attactatag atgggcaaga aaagatgggc aagaaatgga tctgagtcaa acgatgcctt
      781 cttatgctca agtagtgagt ctttatgatg atacaagtat ctctgttagt aataatgaga
      841 cggacaaagt gttggctggc tccctctata ctgatacaaa tgaacaggga ttaacgattc
      901 caagcagttt actaaaaaat tggaatgaac agacaggaaa aaatttgaca gctaatgatc
      961 ttattggcaa atcagtctca gccagcattg tagaaagtgc tgccgaaact agtaagattg
     1021 ctcaatttca aacgaagatt gtacgtgtaa tcaatgatga agatgacatg gaggacagca
     1081 acagtttcat gctgtctcat caaatggaaa cgattttgaa agaggctgga tttacgaaag
     1141 ctgtatctta ttttatcttg gaactcaaag atccatcaca gacaaaagta gtaacagaag
     1201 aattacagaa aaataagaag tatactgtgc tttctcaaca gagagttctt gatattgtga
     1261 ttacctttat tcgtgttatt cagggattat tgattgtgct ttcatcacaa gctattgtgg
     1321 tagcagcggt tatgattggt atcattattt acatcaatat catgcaacgt tccaaggaaa
     1381 taggtgtcat gaaagcagtt ggttatcaga atcgtggtgt caaaggaatt tttatttacg
     1441 aggctatctg gattgtaggc atcgccttgc tgctggcatt tttggttgca caaggggtgg
     1501 gaagtttggc gaatgcgatt gtaagtcact tttacccatc catcactaag gtttttgaat
     1561 taaatctttt atctgtttta ggaactctag ttttcgctct attacttggt tatgtctcag
     1621 cctacttccc ggcgcgtaag attagtaaaa tggatcctgt agaatcgtta cgctatgaat
     1681 aataagggat tccttgattt acagtcttaa aaagaaactc acatagaaaa acaatggcca
     1741 tacttgcaat tcaactcaaa tagttgtata ataggtttgt tgaaaggtga tttgtagtga
     1801 ttcaagttac tcttttcaca ataaaaattt catgattttc ataaggagga aatcactaat
     1861 ggtagttaaa gttggtatta acggtttcgg acgtatcggt cgtcttgctt tccgtcgtat
     1921 ccaaaacgta gaaggtgttg aagttacacg catcaacgac cttacagatc cagttatgct
     1981 tgcacacttg ttgaaatacg acacaactca aggtcgtttc gacggtactg ttgaagttaa
     2041 agaaggtgga ttcgaagtta acggtaaatt cgttaaagtt tctgctgaac gtgatccaga
     2101 gcaaatcgac tgggctactg acggtgtaga aatcgttctt gaagctactg gtttctttgc
     2161 taagaaagaa gcagctgaaa aacaccttaa aggtggagct aaaaaagttg ttatcactgc
     2221 tcctggtgga aacgacgtta aaacagttgt attcaacact aaccacgacg ttcttgacgg
     2281 tactgaaaca gttatctcag gtgcttcatg tactacaaac tgcttggctc caatggctaa
     2341 agctcttcaa gataactttg gtgttgttga aggattgatg actactatcc acgcttacac
     2401 tggtgaccaa atgatccttg acggaccaca ccgtggtggt gaccttcgcc gtgctcgcgc
     2461 tggtgctgca aacatcgttc ctaactcaac tggtgctgct aaagctatcg gtcttgtaat
     2521 cccagaattg aacggtaaac ttgacggatc tgcacaacgc gttccaactc caactggatc
     2581 agttactgaa ttggtagcag ttcttgaaaa gaacgttact gttgatgaag tgaacgcagc
     2641 tatgaaagca gcttcaaacg aatcatacgg ttacacagaa gatccaatcg tatcttcaga
     2701 tatcgtaggt atgtcttacg gttcattgtt tgacgcaact caaactaaag ttcttgacgt
     2761 tgacggtaaa caattggtta aagttgtatc atggtacgac aacgaaatgt catacactgc
     2821 acaacttgtt cgtactcttg aatacttcgc aaaaattgct aaataattct tgagttgata
     2881 gaaagcaagg ctttgtggtc ttgcttttta tatggaaaaa tggatgacac gatcatccat
     2941 tcttttttaa ttctttttca aatgtatttg aaagggtagt gaaagttagc ttctctaaag
     3001 taagtgggtg ggtaaaggaa agtcggaagg catgaagcat aagccggctt gtctttgatt
     3061 tactattata gagagggtct cccaggatag gaagattatg atgcgaaagg tgcacacgaa
     3121 tctgatgggt tcgccctgtc tttagcttgc aatgagccaa ggaagtcttg tttgagaatt
     3181 gctttaatct gcttacatgc gtttcagcat attgcccatt ttttgcatca actattcttt
     3241 ttctacgatc atggcgatca cgtccaattt tgtctctgaa aacaagttct tttctgttga
     3301 tatttccatc aactagagcc caatattctc tagaaatctc ttttttctcc aataagcgat
     3361 tgagaatggg caggataaaa ggatttttgg caaagagaac taagccactg gtttccatgt
     3421 ccagacgatg aacgacatag caggtttggc caacataggt actgacatgg ttaagaaggg
     3481 caatttcgtt tggttgatta ccatgcgttt tcatcccctc tggtttgttt acaataatca
     3541 agtgttgatc ttgataaact tcctgcacta agtctgggtt gccccaaggg atcgtctttt
     3601 gggaataatc ttcctcgtca aaagtcaact ggcaaacatc tccaggattt acgatttcct
     3661 tccagtggac ttcttcttga tttatcaaaa tatgtttctt gattctcaaa aaatgacgga
     3721 tttttctagg gatgaggagt tgttcctcaa gtaattgctt tactgtcatt tgaggtagag
     3781 aggcgggtaa tgtaaatgtg aattgcatac agatattgta acaaaaaaag ccctatttgg
     3841 ataggaaata gctaaattct tgtcttccta tgatgaagat gataaaataa acgcatgaaa
     3901 ttagataaat tatttgagaa atttctttct ctttttaaaa aagaaacaag tgaactagag
     3961 gactctgatt ctactatctt acgtcgctct cgtagtgatc gaaaaaaatt agcccaagta
     4021 ggtccgattc gaaaattctg gcgtcgttat catctaacaa agattatcct tatactaggt
     4081 ttgagtgcag gcttgctagt tggaatctat ttgtttgctg tagccaagtc gaccaatgtc
     4141 aatgatttgc aaaatgcctt gaaaactcgg actcttattt ttgaccgtga agaaaaagag
     4201 gctggtgcct tgtctggtca aaagggaacc tatgttgagc tgactgacat cagtaaaaac
     4261 ttgcagaatg ctgttattgc gacagaagac cgttctttct ataaaaatga cgggattaac
     4321 tatggccgtt ttttcttggc tattgtcact gctggacgtt caggtggtgg ctctaccatt
     4381 acccaacagc tggctaaaaa cgcctattta tcgcaggatc aaactgttga gagaaaagcg
     4441 aaagaatttt tccttgcctt agaattaagc aaaaaatata gtaaggagca aattctaacc
     4501 atgtacctta acaacgctta ttttggaaat ggtgtgtggg gtgtagaaga tgcgagtaag
     4561 aaatactttg gagtttctgc atcagaagtg agtctggatc aagctgcgac tctggcaggg
     4621 atgctcaagg ggccggaact gtataatccc ttgaattccg tagaagattc tactaatcgg
     4681 cgcgatactg tcttgcagaa tatggttgca gcaggatata ttgataaaaa ccaagaaacc
     4741 gaagctgctg aagttgatat gacttcgcaa ttgcacgata agtatgaagg aaaaatctca
     4801 gattaccgtt acccctctta ttttgatgcg gtggttaatg aagctgtttc caagtataat
     4861 ctaacagagg aagagattgt caataatggc taccgcattt acacagagct ggaccaaaac
     4921 taccaagcaa atatgcagat tgtttatgaa aacacatcgc tatttccgag ggcagaggat
     4981 ggaacgtttg ctcaatcagg aagtgtagct ctcgaaccga aaacaggggg agttcgtgga
     5041 gttgtcggtc aagttgctga caatgataaa actggattcc ggaatttcaa ctatgcaacc
     5101 caatcaaagc gtagtcctgg ttctacaatt aagcctttag ttgtttatac gccagcagtt
     5161 gaagcaggct gggctttgaa taagcagttg gataaccata ccatgcagta tgacagctat
     5221 aaggttgata actatgcagg gatcaaaacg agtcgagaag ttcctatgta tcaagccttg
     5281 gcagaatcgc ttaatctacc tgctgttgcc actgttaatg atttgggtgt cgataaggct
     5341 tttgaggcag gcgaaaaatt cggactcaac atggaaaagg tcgaccgtgt tcttggtgtc
     5401 gccttgggaa gcggtgttga aaccaaccct cttcaaatgg ctcaagcata cgctgccttt
     5461 gcaaatgaag gtttaatgcc tgaagctcat tttattagta gaattgaaaa tgctagtgga
     5521 caagttattg cgagtcataa aaattcacaa aaacgggtga ttgataagtc tgtagctgac
     5581 aagatgacca gtatgatgtt ggggactttc accaacggta ccggtattag ttcatcgcct
     5641 gcagactata ttatggcagg gaaaactgga acaactgaag cagttttcaa tccggagtac
     5701 acaagtgacc agtgggtaat tggttatact ccggatgtag tgattagcca ctggcttggc
     5761 tttccgacca ctgatgaaaa tcactatcta gctggctcta cttcaaacgg tgcagctcat
     5821 gtctttagaa acattgccaa taccatttta ccttatacgc caggaagtac ctttacggtt
     5881 gaaaatgctt ataagcaaaa tggaattgca ccagccaata caaaaagaca agtacaaacc
     5941 aatgataata gccagacaga tgataatttg tctgatattc gagggcgtgc gcaaagtcta
     6001 gtagatgagg ctagccgggc tatctcagat gcgaagatta aggaaaaggc tcaaacaata
     6061 tgggattcga tagtcaatct atttcgctaa gatgctgtca atcgaaagtg attatgtccc
     6121 caaaaaatgg agagtttgag ggcaattatt ttgatttcat ttgacacgag ga
//
LOCUS       DAVLYR010000106         6020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLYR010000106 DAVLYR010000000
VERSION     DAVLYR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.106"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            3..158
                     /gene="blpC"
                     /locus_tag="VYO63_001543"
     CDS             3..158
                     /gene="blpC"
                     /locus_tag="VYO63_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1672436.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(202..1542)
                     /locus_tag="VYO63_001544"
     CDS             complement(202..1542)
                     /locus_tag="VYO63_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1672437.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKFLL
                     GIIFTTFQFLAVSKYLSYFIEPLFGIGLSFLLLRGLPKKILIFYGLFPMILVELFYRG
                     VSYFVLPFLGQGIVDGDGNPIFLLIMIFVCFIVLVFLKWLDYDFTRLRREFLDTGFQK
                     SLTKINWAMGAYYLVMQSLSYLEYEQGIQSTTVRHLILVFYLLFFMGGIKKLDTYLKE
                     KLQEELNQEQTLRYRDMERYSRHIEELYKEIRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLRDSSQKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(1556..2293)
                     /locus_tag="VYO63_001545"
     CDS             complement(1556..2293)
                     /locus_tag="VYO63_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672438.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(2298..2636)
                     /locus_tag="VYO63_001546"
     CDS             complement(2298..2636)
                     /locus_tag="VYO63_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672439.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2912..3241
                     /locus_tag="VYO63_001547"
     CDS             2912..3241
                     /locus_tag="VYO63_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1672440.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3409..4458)
                     /locus_tag="VYO63_001548"
     CDS             complement(3409..4458)
                     /locus_tag="VYO63_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672441.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4455..5186)
                     /locus_tag="VYO63_001549"
     CDS             complement(4455..5186)
                     /locus_tag="VYO63_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672442.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5254..5664
                     /locus_tag="VYO63_001550"
     CDS             5254..5664
                     /locus_tag="VYO63_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1672443.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5674..5961
                     /locus_tag="VYO63_001551"
     CDS             5674..5961
                     /locus_tag="VYO63_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672444.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         1990 a         1321 c         1111 g         1598 t
ORIGIN      
        1 acatggataa gaaacaaaac ctaacttcat ttcaagaact aacaactacc gaactcaacc
       61 aaattatagg tggaggatgg tgggaagatt tcttatatag atttaatata attgaacaaa
      121 aaaatacaaa aggatttcat cagccaatac aactataaaa aaacaagacc aagagaacgc
      181 actctcggtc ttattttttc ttcattctat atgtattaca gtaagtacct gacgaaaaac
      241 gtgatcttgg caggtggtat tgagactggt attgggatga ctttccacaa ttttcataac
      301 ggtatagaga ccaactcctc tctcctcccc tttagaactt gctccaaagg agaagatttc
      361 agaaatatcg attccctctt ctttgatgga attttcaatg ataaaggtct cctgtgctcc
      421 atttttaaaa aaggcaattg aaacatgagg ttgacaggct tctacactag cttcaatagc
      481 gttgtcacaa aggatagaca caacggttag aaaatcaagt agactcatcc cctctacctg
      541 aatctcctct ggaacttcga cattaaagac aatgttctta tctctagctt ttataaattt
      601 tccagctagg agacttttga gggcacgatc ccgaacattc accaatcggc caaggtcata
      661 tttattgtcc tgcaatttct gactggaatc ccttaagacc gagtcgtaga tctcttttat
      721 ctgctccata tcctcctctt caatgcccaa acgtaagctg gttaagaggt tagtgtagtc
      781 atggcgaaaa ctccgaattt ccttgtaaag ttcctctata tgccgactat agcgttccat
      841 atctctgtag cgcaaggtct gctcttggtt cagttcctcc tgaagttttt ccttcaaata
      901 ggtatccaat ttcttgatac cccccataaa aaagagtagg taaaacacta ggatgagatg
      961 gcgaacagtc gttgattgaa taccttgttc atattcaagg taagatagac tttgcatcac
     1021 tagataataa gcccccattg cccagttaat cttagtaaga gacttttgaa aacctgtatc
     1081 tagaaactcc cttctcaatc tagtgaaatc atagtctaac catttcaaaa agactaaaac
     1141 tatgaagcaa acgaatatca taatcaataa aaagatagga ttgccatccc catctacaat
     1201 tccttgcccc aaaaatggaa gcacaaaata ggaaacacct ctgtaaaaga gctctactaa
     1261 tatcattggg aagagaccat aaaaaataag gattttttta ggaagccctc ttaacaataa
     1321 gaaagataga cctataccga acaaaggttc tataaaatag gacaaatatt tacttacagc
     1381 caaaaattga aaagtcgtaa aaatgatccc tagaagaaat ttcagaagaa aggccttaaa
     1441 aatcctctcg aaagtaagat caattccatc caccttaaag aagatgacaa tttctagtcc
     1501 attagtaaca agtgtataca acaatatcca agcaatgttc ataaattctc ctagctcagt
     1561 gtaatttatt gatggcctca gacacttccc tgaccttata acgggcgatt agacaacttc
     1621 caccattggg aaagaaaagc agtttttctt tcttatccaa atgcaccaca tttgcaggat
     1681 tgatgagaaa agagcggtgg cactgcaaga gacggggctc ctgcttgaaa acctcctcta
     1741 aactcgctgt aaattccagc ctgtctgtct tggtatagag aataacacga tgggctctgg
     1801 gcgacgtttc gagatagtaa acctctttaa aaggatactg aaattgggca aattttgatt
     1861 taaagtaaaa gcaatcttcc gccagacttt tactatcttg actattggca tagaggaggg
     1921 ctgtctcgat ccgagattca aactcctctg ctgacaaggc cttatcaatg tagtccaaag
     1981 cagacacttg gtagcgaaaa gacaggggca taaactccga gtgagtcgtc acaaagacaa
     2041 tcagggcata aggatcccga tcccgaatct ttctagccac ttccagtccc ttcatctctt
     2101 catttcgaat ctcaatatcc aaaaagaata gctgatgggc ccccttctca tgcacttcag
     2161 ccagcagttg gtccggcttg ccaaatacct caaaagagct aggaatgata tgatgtgctt
     2221 tcaaaagttt ctcaatcgtc gtttcaattc tagtctgttg ggaaaaatca tcctctaaaa
     2281 caaatattct catcttctta ctctccctct ttcaaccatt tttgacgaat ttctctatag
     2341 cgacgtctgg catacttgac agcgtattga ccttcttctc cgagaaaaag gattctttct
     2401 tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac aatttcgatg acatctcatc
     2461 aaggtttccc cacattggtt ctcaagatta cttaaatttt gaagcatatt aaaactagct
     2521 tcttctgtaa caatctgtac ggtatgggct ttagttggat gtgtttggat atagtagata
     2581 tcgtcgatgt ttactttata gactgttttc tgcgtctgaa taatcatata tttcattgga
     2641 ttctcctcct aagaaaagaa taacagaaaa ggtctagccc aagcacaccc aatcctaaac
     2701 agtctttgaa aactcctaaa aggattattt tctacaacat catgatgagc ttacaactat
     2761 tttatcataa aattcactca gcgcaattca agacatttca atgacaatta aagatttgaa
     2821 taaaaaaaaa gaattaaaag tgataaacta acagtatcaa aacacagttg ccagaagtaa
     2881 gactggcacc cagattaaag gaggcattcc catgacagat acagacccca tcaaaagagc
     2941 tcatactttg attactgact taaacaaagc ctaccaagca tgcaaacagg caagcgctga
     3001 cgacgtccgc tttcaggagc aattaaactc tattcttggt tttctagcca aggctgaaac
     3061 agtggataat cgattcttga ttgaactaga aaaattttac cagacttcca gtcttctcat
     3121 gggactcagc gctcttgatc cagatgctcc aactcgcgcc gcttggcggg cctatgaccg
     3181 cttccacttt gaccaagtca agaccaagtt aatactcaat gaaaatcaaa gagcaaacta
     3241 ggaagctagc cgcaagctgt acttgagtac ggtaagacga cgctgacgtg gtttgaagag
     3301 attttcgaag agtataagtc tctacggacc aaccattatc ctgtagtaac aaaaaagaag
     3361 ttgagacaga ctgaactcaa cttctttttt agtgtcgtat cagcaatgtt agtcctgcat
     3421 ctgacgtttt acctgataag gcaaatacaa gactagtaaa accaaaccag ctcctagtaa
     3481 ggctagaagg gctagttttt cttggaaggt aatcaaccca acaactaatt ccacaagtaa
     3541 aacaaaactg gtcaatcctc gaactacctc ctgtaagcct ttttcctttt gccccttgtc
     3601 cagcggaaag agttgggtca aatactggta gtcaaaggca tgatagaggg ccagcaactg
     3661 gaagagcaag aggtagttaa agagaactac cactgctgtc gcaatccaag cttgctcgat
     3721 aaaaacctgc gccagtacgg aaagtaagag aagacgaaga ctgagagcaa agaggtcgcc
     3781 atttcgcaga taagaacgca gatagagatt ttgccaaatc ttcccaggca ccttctgaac
     3841 agcctttaag ataaagtcca gataggcacg acgcttaacg ctgtttgaaa ttcccttgac
     3901 ctgcgtaaag agggcaaaga aacgaagcaa gacttgctta cgcttgcttt cttgggaaat
     3961 gacatagtcc cagtccagcc cagtttcagt gaaaaatttg ctggcctttt gacgaaagtg
     4021 gaaatatttt cctaccccca ataaaagcac atagagcaga aaaactggca agccataacc
     4081 cattgctaaa aataaaggcg caaataacag caagaaaagg gtctgtacaa agagccaaaa
     4141 gactagggaa atgccagttt gacgcttgag atggagcttg atttcctctt ctccaactaa
     4201 gagaaagagc ttgtctggag cctccatata ggtggcaatt cctccccaaa gtaaaagtaa
     4261 aacagacgta attcctacaa acaaaaggat aggccaatga ttttcaggaa aatgttgtaa
     4321 gagttgactg tactggtagg ctagaaagcc caacaggaca agcaggaaca agacaaagtg
     4381 gtcattgagc acatagcgca gataaccaag acactcctta cgaaaggcct gctttctctt
     4441 taaaaacaag tctttcatag atcctcctct ttggtcagag ccaagtaaat atcattcaaa
     4501 ctagcctcag gcatatcaaa ggcttcacgt agttgcagga gattgccttt ggcacgcacc
     4561 tctcccttgt gaagaatgac aaaggcatca cacatcttct ccgccgaatc cagcacgtgg
     4621 gtactcatga gaatagactt gcccttttgc ttctccactt ccaaaagctg aatcaaatca
     4681 gaaatagcca gcggatcaag accaaggaaa ggctcatcca cgatgaaaag acttggatcc
     4741 accacaaaag cacagataat catgaccttc tgcttcatcc cttttgaaaa atgaacaggg
     4801 aaccagtcta atttctgttc caaacggaac atttttaaca agggctctac tcgttcgaaa
     4861 gccacttttt gctcaatacc gtaagccata gcaaccgttt cgatatgctc tctgagggtc
     4921 aattcctcat acagactagg cgtctcagga atgtagccaa tctgcttgcg gtagctagtc
     4981 gcatctcctt gcagagtcag gccattgata ttgatggagc cactataagg tgccaacaga
     5041 ccgataatct cattgatcgt cgttgatttc ccagcaccat tgagaccaat caaaccgacc
     5101 aactgcccac tttcaacagt aaaggacaca tctttcaaaa caggaacatg aacatagcca
     5161 cctgtcaggt ttttaatttc taacatattt tctccaaatc tggtataatg tagctatatt
     5221 atatcaaaat tcagtacagt agaggtagat tttatgtcag attgcatttt ttgtaaaatc
     5281 atcgcagggg aaattcctgc ttcaaaagta tatgaagatg agcaggtcct tgcctttctt
     5341 gatatctctc aagtaacact aggacacacc ttggtcgtgc caaaagaaca ctatcgcaat
     5401 cttttggaga tggatgctac gagcgccagc caactctttg cccaagtacc aaaagtagct
     5461 caaaaagtca tgaaagtcac taaggctgct ggtatgaata tcatttccaa ctgtgaagaa
     5521 gtcgctggtc aaacagtttt tcatactcac gttcaccttg tgcctcgcta cagtgctgac
     5581 gatgacctca agattgattt tatcgcccac gaaccagact ttgacaaact tgctcaagtc
     5641 gctgaaacca tcaaaaacgc ttaaggagtt gctatgaaat tatccaacct actgctattt
     5701 gcaggagctg cagccggaag ttatctggtt acaaaaaatc gccaaaccat cacagatgaa
     5761 gtcttgaata ccactgaccg cgttcaagct atcaaggacg atgtggatat tatccaaaac
     5821 agcctgcaaa tcattaacca gcaaaaagaa cttatcaagg aataccaaga agacttgact
     5881 tacaagttta aggtcttgga aaaggatatc cagactagac tagctgtgat aaaagaaatg
     5941 cagggaactg aagataagta aaaagagccc gacggctctt ttttaaaacg taaacaagtt
     6001 atagtgaatt gaatttagaa
//
LOCUS       DAVLYR010000107         5984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLYR010000107 DAVLYR010000000
VERSION     DAVLYR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.107"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(90..494)
                     /locus_tag="VYO63_001552"
     CDS             complement(90..494)
                     /locus_tag="VYO63_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1672445.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVIGQRSEERRVRI"
     gene            complement(564..2045)
                     /locus_tag="VYO63_001553"
     CDS             complement(564..2045)
                     /locus_tag="VYO63_001553"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1672446.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWETYVHYPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDPAVQ
                     ILAETDRTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(2064..3242)
                     /locus_tag="VYO63_001554"
     CDS             complement(2064..3242)
                     /locus_tag="VYO63_001554"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1672447.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKETVGKHYEEVVGYAPSFYIAEVAGGTHVLD
                     "
     gene            3407..4414
                     /gene="galR"
                     /locus_tag="VYO63_001555"
     CDS             3407..4414
                     /gene="galR"
                     /locus_tag="VYO63_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1672448.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(4460..5497)
                     /locus_tag="VYO63_001556"
     CDS             complement(4460..5497)
                     /locus_tag="VYO63_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1672449.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(5498..5851)
                     /locus_tag="VYO63_001557"
     CDS             complement(5498..5851)
                     /locus_tag="VYO63_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1672450.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
BASE COUNT         1720 a         1475 c         1133 g         1656 t
ORIGIN      
        1 ttgtatttca tcactctctt ttatatttcc ttctaacata tctcataaat taaaaatgag
       61 tgagataaat ttatcccact cacctttctt tatatcctta cccgtctttc ctcagacctt
      121 tggcctatga caaacatggt aaaggttgct ttgcagacat ttctgccctc ttgattggtg
      181 atatccacat ccatcacaca ggttgtacgg ccttgatgga cacattctcc tttaatggtc
      241 aatacatcgt cgagttttcc tgccttaagg tagttgatag aggattggag tgtcacccca
      301 tcaagtccca gcgagataac caccaaacca ctgatttggt cacatagcgt gaatagatag
      361 ccaccatggg cattgccata gtagttgagc gacgagttca ctactttggt cgtcaccaca
      421 acgtgaccat ctctcatttt ttcaatttcg taattttcaa aggcagatat agcgtcaaaa
      481 tgaaagtctt tcatcgtttc ctcctgatat ttcaaacgac actatgatac tactttttat
      541 aggactgtgc aaggagaaag ctcctagtct agtaaaattc cgacatgttc cacaaagcgc
      601 ataaaggctg tctgcccttg ttctgtctgc ttgtagactc ctgcatcctc aagtacacgc
      661 gcaaagatag cacccacaga gtccttgacg attgcaaggg ctttttcttt atccgttagg
      721 tctggatgtt gggatttgag ttggtctgcc cactcctgat gataatcggc aactgtaaca
      781 gcttctccta caagatagct cgcgacttgc tccacttctt ctttcagacg tggtggcaag
      841 attgccaagc ccatgacctc aatcaagccg atattttcct tcttgatatg ttggacatcc
      901 ttgtggggat gatagatacc atcaggatac tctgctgaag tctgattgtc tcgcaagacc
      961 aagtccaact caaactgtcc atcgcgtttg cgggcaatgg gtgtgatagt gtgatgcggt
     1021 gtcctgtctg tctctgccaa aatctgcact gcaggatctg aatactggcg ccattcctgc
     1081 aaaatcttat cagccaaatt gatcaaatcc tctttggaat ccgaagtcaa acgtaggaca
     1141 gacatgggcc acttgacaat tccagccttg acctgctcaa aaccagcaaa tcggaaggcc
     1201 ttttgcaagg gagccaattc cataggaaat acgtgacggc ctccctgata atgatcatga
     1261 gttagaatag agccccccac aatcggcagg tcggcattag atccagcaaa atatcctgga
     1321 aactggtcta cgatagccaa cagacgttca aaactctgac gactaatggc catgggacga
     1381 tgctggccat ctaaaaagat acaatgctca ttaaagtaag catagggcga atactggaaa
     1441 ccccattcct gaccaaccat ttcaaaacgg ataatacggt gattgctacg agctgggtgg
     1501 ttaactcgac catggtagcc ctcattctct agacaaagct gacactgagg ataattacta
     1561 gcttgcacca acttggctgc cacaatctct ttcggatcct tttcaggctt agagagattg
     1621 atggtaattt caagttctcc ataatcagat ggaacacgat aagcgatatt tttagcaatg
     1681 gccttgagtt tgatgtagtc atttttctga ctgagttgat aaaaatcctc tatcgcttgc
     1741 tcaggatagt ggacataggt ttcccagaag tcacgattga cctgactcgg acaaggagtc
     1801 accaagtcca tcagttcagt accaaggatt tcacgcgcag tctgactatc ctcaatcgtc
     1861 tctaatcgaa cggcttcctc aactagctgg tccttgaggt caatcaattt atccagattg
     1921 gtctcaactt ccaaaacacc ttctcccact cgtgccaaaa cacgattggt caggtagatt
     1981 cgatccattt cctcaaatga actttcagaa atgacatgtg ttacaaattt atctactaag
     2041 gtcaccatag agcctccttt tgactagtca aggacgtgag tgccacctgc aacttcagcg
     2101 atatagaagc ttggagcgta tccaactact tcctcgtagt gtttgcctac agtttcctta
     2161 aaggcctcaa cagtatcttt ttgaaccaag gcaatggcac agccaccaaa accagcccct
     2221 gtcatacgag caccgagaac tccttcttgt gcccaagctg tgtgaacaag agtatccaat
     2281 tccaaaccag ttacttcata atcatgctcc agagaaacgt gtgacgcatt catcaaacga
     2341 ccaaatgttt ccaaatcacc tgcttgaagg gctgcttgag ctttaagggt acgttggttt
     2401 tcaagcacag catggcgagc acgtttcaaa cgattttcat ctttaatcaa atagctatat
     2461 tggtcaacgg cccactcgtc caattcaccc agggtctgaa tatccaaggc aacttgcaat
     2521 tcttccactg ctttttcaca ctcagcacga cgttcattgt atttagagtc cgccaattca
     2581 cggcgtttgt tggtgttcat gataacaacg acattgtcct tcaaatcaag tggcaccaaa
     2641 tcatactcta aggtgttggt atcaaggtaa atagcacgtt gatcggcccc cataccaata
     2701 gcaaactggt ccatgatacc agagttgact ccgataaagt tgttttctgt ttgttttccg
     2761 attttaacca aatcgagacg ctctaatttt aaatcaaaga gatgctctgc cacgactcct
     2821 gtcaagagct ccaaggatgc tgaagaagac aagccagcac catttgggat attcccataa
     2881 acataaaaat caaaaccttt gtcaatcacg tgcccagctt cttgcaagaa atgaaggaca
     2941 ccttttggat aattggtcca gttgtgctct ttttcaaact tgaggtcagc gagaggcact
     3001 tcgataatgc ccttgtcctc aaagttagct gagtagaaac gcaagacttg gtcgtcacgc
     3061 ttacgagctg caccgtaagt tcccaaggaa atagcagcag gaaaaacgtg cccaccgttg
     3121 tagtctgtgt gttcaccaat caaattaata cggcctggtg aaaagaaggt ttggtctgct
     3181 tcttgaccaa aaacagcaag aaagtctttg cgaaggactt cagtagtaag atgttgtgcc
     3241 atatgaattc tcctttgact gtccgttaag ccatcttaac gtgtaatcgt tttctttcta
     3301 ttgtatccaa ggaatttccc aaggtcaatc ctttttacta aaattttact aaaatatcag
     3361 taaaaagaag aaaaatatga tatactaacc taatcaagga ggatttatgg ctaccttaaa
     3421 agacattgca cagctagcct ctgtctcaat cgcgaccgta tcccgtgtcc tcaaccgcga
     3481 ccagagccta tctgttacag aagaaaccag acaccgtatt ttaaccgttg ctgaagagct
     3541 aggctacacc aaacacctca agacaggcga gtcccacaag cccaagcaaa agattgccat
     3601 tatccaatgg gtcagcgaac aaggggagct agacgacctc tactactacc agattcgcct
     3661 aggaatagaa aaaagagccc aagagttgga ctatgatatc ttgcgctatt ttaatgacca
     3721 tccttttacc ctaagcgagg aagtgattgg gattctctgc atcggaaaat ttagccgagc
     3781 tcagatttct gcctttgaag aataccaaaa gcccctggtc tttctagaca gtgatacact
     3841 ttccctgggg catacttgta ttattacgga tttttacact gctatgaaac aggttgtcga
     3901 ttatttcctc agtcaaggga tggaccgcat cgggattcta acagggcttg aggaaacaac
     3961 agaccaagaa gaaatcattc aggacaagcg tctagaaaac ttcaaaaact acagtcaagc
     4021 gaggggaatc tatcatgatg aactggtctt tcaaggaaga tttactgccc agtctggcta
     4081 tgacttaatg aaggaggcca ttcagagctt gggagaccaa cttccgccag catttttcgc
     4141 agccagcgat agtttagcta tcggtgccct ccgtgccctc caagaagctg gaatcagcct
     4201 gccagatcgc gtcagcctca tttcattcaa cgacaccagt ctgaccaaac aggtctatcc
     4261 tcccctctct agtattacag tttatactga agaaatgggg cgagcaggta tggatattct
     4321 taacaaggaa gtcctccacg gtcgcaaaat tcctagcctg accatgctgg gaaccagact
     4381 gacattaaga gaaagtaccc taaatcaaga ataggataac ataaaaaacg aatagagttc
     4441 taaaactcct attcgttttt tattcgatta caatcataga cttaattgtc ttacgttcat
     4501 ccatatcttt ataggcttgg tcgatatctt ccagtttata acttgaagta aagacgcgac
     4561 ctggattgat atcaccatca aggacggctt ttagtaaaaa ttgcttatcg tatgttgtag
     4621 cagaagctgc cccacctgct acagagatat tttgcataaa tgtcgaacca agagcacgat
     4681 tattatagtg tgggactcct acaaagccca tacgccctcc attatgaaga acacctagcg
     4741 cctgttctat agcagcctcc gtaccaacac attcaagtgc tgcgtctgct cctccgccga
     4801 ggatttcacg caccttggta attccttctt gaccacgttc tgcaacaaca gctgtcgcac
     4861 ctgactccat agccatcttt tgacggtctt catgacggct cataaggata atttgtgatg
     4921 ctccacgcat cttagccgcg atgacagcac attgaccaac agccccatca ccgataacaa
     4981 caaccttgtc ccctttttga acatttgcaa cacgcgctgc atgatagcct gtcggcatga
     5041 catctgcaag agtcaaaagg gacttgagca tcccttctgt ataatcagaa ggttgaccag
     5101 ggattttaac cagcgcccag tttgcatagt ggaagcgaat atattctgcc tgaaaatcac
     5161 cccccaaatt attaccaata tgattgtcgc aagaaccgtc aaatccagca agacaggcat
     5221 cacactcacc acatccatgt gtaaaaggga caatcacaaa atcacctggt ttcaccgtcg
     5281 taatggcttc cccagcttct tcaacaatcc caatcgcttc gtgtccacta tttttatgtc
     5341 cagctttcgt ttctggatta cggtacctcc ataaatctga accacaaacg cacgcacgaa
     5401 ccacacgaat aatcacatca tccgcttcta ttatttgcgg acgttcaatg ctagcaagtc
     5461 caacctgacc tgcctttgta tatactgctg atttcattta aaattttcct tccttataaa
     5521 gtttaatttt gagatttaaa cgatttaaag ctgtctgtag ctgagacaag cgctcctcta
     5581 atttttgctt ttcctcttct aaaataccaa gcctctcctc tctcgtttca tctccctttt
     5641 ggtagagcga catatagtca actaaactat ctacagagac acccgccgaa cgaaaacact
     5701 taataaattc cagcgcttcg atatcctgat cttgaaaatc acgaatccca gtagcagtac
     5761 gagtaatcgg tggcacaaga ccaacccgtt cataataccg aatcgtatcc gctgaaattc
     5821 ccaacaaatc actggcagat ttaatattca cacgctaacc tcctctcatt tcattcactg
     5881 aattcacgct attctaactc acttatctta ttatataact ttgagttgac tccaagtcaa
     5941 gcattttgta tctaatcgaa aaatttttat cattaacaat cata
//
LOCUS       DAVLYR010000108         5968 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLYR010000108 DAVLYR010000000
VERSION     DAVLYR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.108"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(180..1700)
                     /locus_tag="VYO63_001558"
     CDS             complement(180..1700)
                     /locus_tag="VYO63_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1672451.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDSIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(1693..2421)
                     /locus_tag="VYO63_001559"
     CDS             complement(1693..2421)
                     /locus_tag="VYO63_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672452.1"
                     /translation="MIEYKNVALCYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(2435..3199)
                     /locus_tag="VYO63_001560"
     CDS             complement(2435..3199)
                     /locus_tag="VYO63_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1672453.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGATYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(3203..3646)
                     /locus_tag="VYO63_001561"
     CDS             complement(3203..3646)
                     /locus_tag="VYO63_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1672454.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(4328..4624)
                     /locus_tag="VYO63_001562"
     CDS             complement(4328..4624)
                     /locus_tag="VYO63_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1672455.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(4646..5710)
                     /gene="pepA"
                     /locus_tag="VYO63_001563"
     CDS             complement(4646..5710)
                     /gene="pepA"
                     /locus_tag="VYO63_001563"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1672456.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALLGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         1714 a         1423 c         1050 g         1781 t
ORIGIN      
        1 aatagtcaac tgtctttaca gtaaaatttt agaaaatcat gaaaattttc tctttctttc
       61 cattttaagt gacattcagt cattctcaca tcaaaaaagc ccagacgaaa ttgtctgagc
      121 attcttttat ctagtcgttt aaggaagttg agttcagtat gtttaaagtc tctgtcccat
      181 catttcttca acaaaccttg ttcttggaga aactccttgg ctacttgctt tgctgacttg
      241 ccttcaacac cgacttggta gttgagctgg ctcatctggc tttctgtaat cttaccagcc
      301 aatgtattaa gaactctttc caactctggg tgtttcttga gaagagcttc tttcatgagt
      361 ggagcccctt gataaggtgg gaagagttgc ttgtcatctt ccaagacctg taaatcataa
      421 cgctccaatt ccgcatcagt cgaataggca tccgtgattt gaatatcccc tgactgaatt
      481 gcctgataac gaagagctgg ctgcatagtc gccacattga gattgagacc atacattgat
      541 tgcaagccct tatttccatc ttcacggtcg ttaaactcaa gcgtaaagcc tgccttcagc
      601 tgcccttcca cttttttcaa atccgaaatg gtcttcaagc catattcttg cgcaatcttt
      661 ttcggaacag ctacagcata ggtattttga taagacatag gtttgagata ggctagatga
      721 tcctgcttgg caatgctatc acgcgccact tgataaacct gctctggctc atgactcacc
      781 ttgggtgatg gttgaagcaa actttcagtc accgtaccgg taaattcagg ataaatgtca
      841 atatcacctt ttttcagagc ttcataaagg aaacttgtcg tcccaaaatt cggtttaaca
      901 gtcgcagtca tgctggtatt ttcttcaatc agcaatttat acatattagc caaaatttct
      961 ggttctgggc ctattttccc agcaataatc aagttttcct tctctttttg agccaaaagg
     1021 gctggactat aagacagacc cagtaataaa gccaccaagg caaaaccaga gaaaatcgtc
     1081 cgcaatttcg ctttttccat tacttttagt aggaagttaa aggcaatagc tagcactgca
     1141 gaagaaaggg ccccaatcaa aatcaaactg gcattattac ggtcaattcc caacaggata
     1201 aaggaaccca gtccccctgc cccaatcaag gtcgccaagg tagccgtacc gataatcaag
     1261 accgctgccg tccgaattcc agacatgata acaggcatgg caagtggaat ttcaaacttc
     1321 ttgagacgct cccatctggt catcccaaag gcaatcccag cctcttgaag actcggatca
     1381 attcccttca gcccagtaat agtattttgt aaaatcggaa aaatcgcata aatcactaga
     1441 gctgtcaatg ccggtaaagt cccaattccc atcaagggaa taaagagtcc caacaaggcc
     1501 agagacggga tggtctggaa aatccctgca atctgtaaga cccaatccgc caacttctca
     1561 tgatagcgaa gaaaaacagc caagggaatc gcaagcaaaa tagctagtaa caaggtcaaa
     1621 agtgacaact gcaaatgttg agatagagct gtcaaccaat cactaaaacg atcctgaaaa
     1681 gttgcaatta aattagtcat gaacactacc tccaaacaag tctgctacaa agtctgttgc
     1741 aggcgctttt aaaattgtct cgggattcgc tacctggcga atttctccat cctgcaagac
     1801 agcaatacgg tccgccaact tcaaggcttc atccgtatca tgggttacaa aaatcgttgt
     1861 catcccaaac tctttatgca attcttttgt cagaacctgc aactgttttc tcgaaatagc
     1921 atccaaggcc gaaaagggtt catccatgag gaaaatcttg ggctgaccaa tcatagctcg
     1981 gacaataccg acccgttgct gttctccacc agataattca ctaggtaagc gatgcccata
     2041 ctcggctact ggtaaaccaa ccttagccaa aagctcttct gttttcttcg taatttcttc
     2101 cttgctccac cccttcattt caggaatgag agcaatattt tccgcaactg ttagatttgg
     2161 aaaaagagca atagcctgta aaacataacc agtagaaaga cgaagttcac gctcatcata
     2221 gtctttgatg cgcttcccat ccatataaat atttccatca gttggttcca aaagacggtt
     2281 aatcatcttg agcatggtcg tcttacctga cccagaaggc cctactaaaa ccataaattc
     2341 cccatcctca atctgtaagt tgacatctct caagacatcc ttttctgtgt agcatagtgc
     2401 tacatttttg tattcaatca ttctttgtcc tcatttaaaa cttccctcga ttggtcaagt
     2461 cttctacctt aggcatgact tccttattat cccaatgctc cacaattttc ccgttcttta
     2521 aacggaagat atcgtactgg gcataagcaa cgccatcaat ctgagtctga ccatagctaa
     2581 ccacatagtt tccttgtcct aagagttgga aaacaaagtc aaaagtgaca ctatattcag
     2641 ccacatagtt tttataagca gcacttcctt gtccaatatc atgattatgc tgaatcaaat
     2701 cgtctgccac ataatcactc cactgctcta gctccccatt ttggaaaatt tctgtcaaga
     2761 aacggcgaac cagcttttta ttttctgctt tcttgtccaa atccttgatt tcaaaatctc
     2821 caaaaatttg atctagttgg tcattttcag gtgttcgata gtagtcaatg acatcccaat
     2881 gctcaacaat acgaccattc tcatccgcac gaaaagtatc cgtcgtcacc cattgagctt
     2941 ctccaccatt cagatattga tgaacatgaa caaagaccag attgccatcc tcaatggtgc
     3001 ggacaatctt aatctgacgc tctggatgac gctcaaagaa atctgcaaag aaggctgcaa
     3061 atccttcttt cccgtcagga acacctgtcg aatgttggat ataggtagcc cctacagact
     3121 gggcttgagc ctcagcaact cgtccgtctt gaatggcatg gatgtatagg ttgtgagcat
     3181 ttttcacttg ttgtgacata ttctaaacct catttccctt ctctttcaga ttcgccaaaa
     3241 ttctttcttg aaaaccttca aattggtgaa tttcttcctc tgaaaatcct ttgtaaaaga
     3301 tagtatccaa tttctgactg acacgatgcc ccacttcttt ctgggacttg cctaactccg
     3361 ttaaaactaa atacttctta cgcttgtctt ttccacacgg actaacaatt acaagctttt
     3421 gttcctctag cttttttatc atagtcgtca gcgtattatt cgcaagtcca gtcgcaagcg
     3481 cgatatctgt cgcagttgcg cagccagttt cactattcca taaaaccgct aaaatcttgc
     3541 cctgttcacc cctataaaga gcctcaggat cttgactcag taacttttga aaaatccgcc
     3601 cattcaacaa acgaatatga tgggctagca aatgaccatc tttcataaca cctccaattt
     3661 atttcgatat cgaaatgaat aaaacaattg taacactcat cgttctaact gtcaactatt
     3721 tcgatttaga aataattttt gataattatc cacaccacca tactccggct caactaactt
     3781 ttaacgagag tttctaaact ccttcgtcct ccagtctaca aaagccttcc atccgtacta
     3841 tcctatattt tatgagggga cacatttttc ctatcagacc atttatttta aagatagaag
     3901 taaatcataa ttgcttccat ctgttctttt atagtatatt gaagttggac tagggcactg
     3961 tatcttctaa aacattgata gaaagcgatt tgaatttccc aatcaatttg ttcgtcttta
     4021 tagcatttcg aaactggaat aggacaccat gactgctaaa agatttctat aaattcattt
     4081 gatttcctca atcaatttgt tcatatctta tttcattccg ctataatttc accttaccct
     4141 atctttttcg tagcaccctt caaacagcct atcccctacc gtttgacgat tcctcacttc
     4201 actccacttc cattacagaa gtttcttcac tactatggtc tcggctgact tctcatgatt
     4261 ccttgttact actatttgaa cgctcacgag ataggtctta taaaaaatgc tttgatccac
     4321 aatggaatca aagcatttta aaaagttcct catacataag tgcagaagtc gcagttcctc
     4381 tgtacttggc ttcttctctt ttgataaagc gagccaagtt gagcaactca ggtgctggat
     4441 gtttgggatt taggagcaat tcacgattga ccaggcctga gagacgaact gcctgcaatt
     4501 gctcatttgt agtaggcagt tttttagtag tctctaggag agcagcaact aaatcttcac
     4561 tcaaatcatg tcgagcatga ttgtaaagat cttttataag gctttctagg tttggttctg
     4621 ccatccctac cacctccctt atggtttaat aatgtttaat caaatcaacc gttgaacgat
     4681 ccaatttctt caccaaggct tgtaagaaag cttgcgcttc taggaagtca tccattgcat
     4741 agagggtttg gtgagaatgg atataacgag cgcagacacc gatagttgtt gatgggacac
     4801 caccattttt cagatgagct gcacctgcat ctgttccgcc tttaccacag tagtattggt
     4861 acttgatacc agcttcttca gccgttgtca aaaggaaatc cttcatccct gggagaagca
     4921 agtgacctgg atcatagaaa cgaatcaagg ttccatctcc aatcttgcct tgaccaccgt
     4981 agacatcacc tgctggtgag caatcaactg cgaggaagac ttctgggtca aacttggttg
     5041 tagaggtatg agcgccacgc agaccaactt cttcttggac gttagaaccc agatagagtt
     5101 cattgccgag tttttgacct aataaagctt cagctagctc gcttaccatg aggacaccgt
     5161 agcggttatc ccaagctttt gagatgatat ttttttcatt ggctgtcaaa attgcagaac
     5221 tatctggtac gatggtatca ccaggacgga tgccaaaact ttctgcctca gccttgtccg
     5281 caaaaccacc atcaaaaacg atatcggcaa tggctggcat ggttggtccc ccctttccac
     5341 gagtcaaatg cggaggaaca gaacctgaaa tcacaggaat ttcatgacca tcacgagtca
     5401 agagtttgaa acgttggctg ctaaccacca tggggttcca gccaccgatt tctacgacac
     5461 ggaaggtacc atctggcttg atttcgctga ccataaaacc aacttcgtcc atatgagaag
     5521 cgaccaagac gcgcggtgca tccacagctt ctgaatgttt gataccaaaa ataccaccca
     5581 agccatctgt caccacttca tccacatgcg gtgtcaactt ttcacgaaga taagcacgga
     5641 caggcgcttc atgacctgag actgcagcaa gttctgttac ttctttaatt tttgaaaata
     5701 atgttgtcat ttcagttcct tctttctttc atccatttta ccacttttta taggagaagg
     5761 atagtgggaa ggtggatttc taagttagta tcttagtcct gctctatctt agaaaaggat
     5821 agtattctct tgcatgtagt gcaaaatcta gtaaacattc caaaattaac tcgaatattt
     5881 atttccaaac aaaaaaacaa tacaccatca aagttgtttg gatttttcat gaaatttaca
     5941 gaaaatagtt gacttccctt tcttcttt
//
LOCUS       DAVLYR010000109         5965 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLYR010000109 DAVLYR010000000
VERSION     DAVLYR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.109"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            76..1479
                     /locus_tag="VYO63_001564"
     CDS             76..1479
                     /locus_tag="VYO63_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1672457.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELNSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            1594..2232
                     /locus_tag="VYO63_001565"
     CDS             1594..2232
                     /locus_tag="VYO63_001565"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1672458.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            2244..2684
                     /locus_tag="VYO63_001566"
     CDS             2244..2684
                     /locus_tag="VYO63_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1672459.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            2677..3108
                     /locus_tag="VYO63_001567"
     CDS             2677..3108
                     /locus_tag="VYO63_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1672460.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            3099..3569
                     /locus_tag="VYO63_001568"
     CDS             3099..3569
                     /locus_tag="VYO63_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1672461.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            3594..4367
                     /locus_tag="VYO63_001569"
     CDS             3594..4367
                     /locus_tag="VYO63_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1672462.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            4364..5164
                     /locus_tag="VYO63_001570"
     CDS             4364..5164
                     /locus_tag="VYO63_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1672463.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            5223..>5965
                     /locus_tag="VYO63_001571"
     CDS             5223..>5965
                     /locus_tag="VYO63_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1672464.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSL"
BASE COUNT         1966 a          784 c         1293 g         1922 t
ORIGIN      
        1 gtaagcgttc acatattgaa aaaataaaat gtggggatta taataaactt aatcaaggac
       61 gaagagagaa gaaaaatgga agcggtttta gcaatagatt taggtgcgac ttctggaaga
      121 gcaatcgttg gttacctttc tgaaaataaa ctagtaatgg aagaaataaa tcgcttttct
      181 aatctaccta ttagagtaaa agggcattta tcttgggatg ttgactttct actagctaaa
      241 attcttgaaa gtatccgctt ggctaatact agttacaaga ttttatctat cggtattgac
      301 acatggggag ttgattttgg cctgattgat aatgaaggta agctgttatc acaacctgtt
      361 cattatcgtg atgaaagaac aaagggagtg ttaaaggaaa tatctgaaat gactgaatta
      421 gaaaaactgt attcagagac aggaaatcag attatggaga taaatacctt gtttcaactc
      481 tttaaggcac gtcaagaatc tcctgactct ttctataaga ccaataagat tcttttaatg
      541 ccagatttgt ttaattatct cttgacaggt aagtttgcta cagaaaaaag cattgcttca
      601 acaactcaat tatttgatcc taggagtcaa aattggaatc agaatatctt aaaactattt
      661 gaattgaatt catctttact tcctgaaatt gtttcagagg gaaatgttct tggaaggata
      721 aaaaaggagt atggtttagg cgatattcct gttgtgaatg tttgtagtca tgatacagca
      781 agcgcgattg tctcagtacc taagacagaa ggtagtttat ttatttcatc aggtacttgg
      841 tctttggttg gagtggaact tacttcaccg attcttacta ccgaatcctt cagttatgga
      901 tttacaaatg aagtcggtaa agatggagtg attacatttc tgaagaattg tacagggttg
      961 tggatcatag aggaactaag acgttcattt gaacgaagag ggaaagccta ttcttttgat
     1021 gatattagga caatggtgga gaaagaaaaa gaaaatcttc ctctgattga tactgaatca
     1081 actgaatttg caacagaatc tgatatgcac aagactttga cagaatatct agcttatcat
     1141 catgaaacta gagagtggac agatggacaa ctatttaaga ttgtttatga aagcctagct
     1201 gaaacgtata ggaaagcgat agagttacta gaagaactaa ctcataaggt ttataagagg
     1261 atatatgtga ttggaggagg tgctagagcc agttatttta accaaatgat tgctgataga
     1321 actggtaaag aggttcttac aggttcgact gagggtacag ctgtggggaa tattgttgtg
     1381 cagctcatag ctatgggaca attaaaaggg atggaagaag ctcaccatgt tattgaggag
     1441 tttctacaat tagagagtta ttactcccaa aagaattaaa aagattgaga gtttgtaaat
     1501 ttgcctccct cccccttctt aagttttgta caagaagggg ggataattgg tgaattgaaa
     1561 aatatttagt gttttgatat gaggaggaca agaatgtcag atgtaaaaca agaattaatt
     1621 aagtatggca agaagctagt agaaacagat ttgacgaaag gaacaggtgg gaatctcagt
     1681 gttttcgatc gtgaaaaaca attgatggca attaccccgt cgggtattga tttctttgaa
     1741 atcaaagaat ccgatattgt agtgatggat attaatggaa atgttgtaga gggagaacgc
     1801 ttgccatcta gcgaatggta tatgcatttg attcaatatc aaactcgtga tgatatcgat
     1861 gcaattatcc atgctcatac aacttatgca acagtattag cttgtctcag agaaccactt
     1921 ccggctagtc attatatgat tgcagtggca gggaaagatg ttcgggtagc tgagtatgca
     1981 acatatggta cgaaagaatt ggctgtgaat gcagctaaag caatggaagg tcgtagagca
     2041 gttttactag cgaatcatgg aattttagca ggtgcacaaa atttattgaa tgcatttaat
     2101 attgttgaag aagttgaata ttgtgcaaaa atttattgtt tagctaagaa ttttggagag
     2161 ccagtagttc ttcctgatga ggagatggat ttaatggcag aaaaatttaa aacatacggt
     2221 cagagaaaat agggaggata ttaatgttaa aacatatacc gaaaaatatt tctccagatt
     2281 tattgaagac tttaatggaa atgggacatg gagatgaaat agtattagct gacgcgaatt
     2341 atccttctgc ctcatgtgca aataagctaa ttcgttgtga tggtgtaaat attccagaat
     2401 tattagattc cattctgtat ttaatgccat tagatagtta cgtcgatagt tcaattcagt
     2461 ttatgaacgt tgtttcgggt gatgatattc ctaagatatg gggtacctat agacagatga
     2521 ttgaaggtca tggtacagat cttaaaacga ttacttatct tagaagagaa gacttttatg
     2581 aacgtagtaa gaaagcttat gctattgttg ctacaggaga aacttcactt tatgctaata
     2641 ttatccttaa gaaaggagta gttgttgaaa gagaaaatgt ttaatagagg aattttagtt
     2701 gccagtcatg gtaattttgc tagcggagct ctcatgaccg cagaaatgtt tgttggtgag
     2761 acaacaaatg atagagttag gacattaggt ttgatgcctg gagagaatat tgtagagttt
     2821 gaacattatt ttaaaaatca agtaaatgaa ctgttagact caaatcaaga ggttatcgtt
     2881 ttgactgact tgattggagg aagtcctaat aatgttgctt tgtcacggtt tttaaatttg
     2941 gattcagttg atattgtaac agggtttaat atccctctcc tagtggaatt aatatcaaat
     3001 tatgattcaa aaatcaattt agaagaaatt gttcacaatg ctcaaaatag tttgtttaat
     3061 gttaaacaac aacttaacgt agaggaggaa gaagatttat gtctatagag tttgttcgta
     3121 ttgatgaccg tctggtacat ggtcaagttg tcactacgtg gctaaaaaag tatgatattg
     3181 agcaagttat cattgttaat gatcgcatct cagaagataa aacacgacaa tctattttaa
     3241 agatttctgc accagtaggt ttaaaaattg ttttctttag tgtaaaacgg tttgtggaag
     3301 ttttaaactc tgtgccaata aaaaagagaa caatgctgat atatacaaat ccaaaagatg
     3361 tgtatgattc tattgaagga aatttaaaat tggagtacct caatgtagga cagatgagta
     3421 aaacggagga aaatgaaaag gtaacgggag gtgtagctct aggtgaagaa gacaaatatt
     3481 attttaagaa aatagttgat aagggaacga gagttgaaat tcaaatggtt cctaatgata
     3541 aagttacaat gttggaaaaa tttttataaa aataatttaa ggaggtacag tatatgctat
     3601 tcacacaagc attactggtg acattagttg ggattattgc cactattgac tataatggac
     3661 cgttatttat gattcaccgt ccgttagtta caagtgcaat ggttggctta gtcttaggag
     3721 atttcaccca aggtgttctt attggttcag ctcttgaatt aacttggctc ggtgtaacag
     3781 gtattggagg ttacactcca ccagatacta tttcaggtgc gattattggt actgcatttg
     3841 gtattttatc tggtcaagga gaaactgctg gtatcgctat agcagttcca attgcagttg
     3901 ctacccaaca gttggatgtt cttgcaaaaa ctttagatgt ttattttgtg aaaaaagctg
     3961 ataatgatgc taaaaacgga gattattcaa agatcggttt ttatcattat tcaagtttgg
     4021 ttttaatcac gttatttaaa attgtaccaa ttttcctagc tattatgctt ggaggggaat
     4081 atgtggcaga cttgtttgct aaggttccac caatcgttat gcagggactt aactctgcag
     4141 gtgctttact accttcaatt ggttttggta tgcttttaaa tatgatgctc aagaaaaata
     4201 tgtgggtatt cttgttgatt ggattcattt gttctgtgta tggaggaatg tcaaccattg
     4261 ggatctcact agttggtatt gcggtagcat acttctacga tatgattgga agtaaaccac
     4321 aagaaacaac ttcaagtagt gatgttgagg aggatcttga tctatgatga ataataaagt
     4381 aactaaagtt gaacttaaaa aagttttcaa acgaagtttt atgtatggtt cttcatggaa
     4441 ctatgagaga atgcagaacc taggttttct atatacaatt cttccagtat tgaaaaaact
     4501 atacccagac aaagattcag cttctcctgc aatgaaacgt caccttgagt ttttcaatac
     4561 tcatcaaaca gcggcaccat ttattcttgg agttacttcc gctatggaag aacaagaagg
     4621 aaatgaaggt gcagcttcaa ttactggtat taaagttggc ttgatggggc cactggctgg
     4681 tctaggagat agtttgttct ggctgacact agttcctatc tgttttagta ttggtgcgtc
     4741 ttattctaaa gacggcggtg ctttaggtat ctttatcgcc ttaatattgt ttaatattat
     4801 taatattcct gttaaatatt tcggtttgaa atatgggtat actaagggtt ctagtcttat
     4861 ccaagaaaat aatacaaaag gaacattgaa tcgcgttacg agtatggcga cagcattagg
     4921 gctagtacta gtgggtggtt tgattccatc aatggttggt attaattttg gattagaatt
     4981 taagcagggg gaacttgtta tttctgttca agaaatgatt acaaaattaa ttccaggatt
     5041 tatccctatg gctttgactt tattaatgtg taaattaatt agaaaaggaa agaatccggt
     5101 tgtactaatc tttagtgtta tggctattgg agttatccta gttgttttag gaattttgaa
     5161 gtagtagaaa gtgtggaggt ggtatttggg atatcacctc cattttggaa gagaggtaaa
     5221 gagtgaaatt atggtataag aaagctgcct caaattggaa tgaagccttg ccgattggga
     5281 acggtcattt aggtggtatg atttatggtt cagctacaaa agaatgtatt caactaaacg
     5341 atgagactat ttggtataga ggaaagtcag atagaaataa tccagactca ctattgcatc
     5401 ttaaaaaaat tcgggaatat cttttagatg gagaaattca gaaagccgaa gaattgataa
     5461 agttaacaat gtttgctacc ccaagagatc aaagccacta tgaattactt ggggaacttt
     5521 acattgagca tatagatatt cagtcttgtg ctctttcatt gtatgaaaga gagctagatt
     5581 tagatacagc tatttctaat gttgtgtttg agcctaatag ttgtaattta caaataaaaa
     5641 gagaatattt tacgagtttt aataagaata ttttatgttg ccgtatagtg tcatcagttc
     5701 aaaacacatt aaatttaaac attaatttgg gtagaaataa acggtttaat gatgaagtat
     5761 ctaaactgga ttcaagtaca attttaatgt cggcctctgc tggaggtaga aaaggtgttc
     5821 agtttaaagt agtatgtcat tctaaggtta cggatggtga agtaagtgta ttgggagaga
     5881 caatagttat tcggaatgct acagaggtat ttctttatct caaatcaatg acggattatt
     5941 ggggaaatat agatatttct tctct
//
LOCUS       DAVLYR010000110         5944 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLYR010000110 DAVLYR010000000
VERSION     DAVLYR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.110"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..186
                     /locus_tag="VYO63_001572"
     CDS             <1..186
                     /locus_tag="VYO63_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1672465.1"
                     /translation="SAQPQLPITKLKKILLPLPPLALQNEFADFVVQVDKSQLAIQKS
                     LEELETLKKSLMQEYFG"
     gene            246..482
                     /locus_tag="VYO63_001573"
     CDS             246..482
                     /locus_tag="VYO63_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1672466.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            479..892
                     /locus_tag="VYO63_001574"
     CDS             479..892
                     /locus_tag="VYO63_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1672467.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1010..1975
                     /locus_tag="VYO63_001575"
     CDS             1010..1975
                     /locus_tag="VYO63_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1672468.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLVSTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1999..2525
                     /locus_tag="VYO63_001576"
                     /pseudo
     CDS             1999..2525
                     /locus_tag="VYO63_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            2555..5905
                     /locus_tag="VYO63_001577"
     CDS             2555..5905
                     /locus_tag="VYO63_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1672469.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFGENCCVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIEEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         1974 a          945 c         1203 g         1822 t
ORIGIN      
        1 agtgctcagc ctcagttacc aattacaaaa ttaaaaaaaa tacttctccc cctcccccca
       61 ctagccctcc aaaatgagtt cgcagacttt gtagtccagg tcgacaaatc acaattggca
      121 atccaaaaat ctctggaaga acttgaaact ttgaagaaat ctctgatgca ggagtatttt
      181 ggctgatatt ctgccattgt aattacggta atgatttgtt ataatacttc aaaggaggaa
      241 atcagatggt agtaaaaaca agaaaacaag gaaattcaat caccattacg attccaagtg
      301 aatttaatat tccaagtggt gttaaatacg aagcgaaatt gttaccaagt ggtgagatta
      361 tctttactcc tgaagaattg gggcagcagg tttcttatgt atctgatgat gcctttgact
      421 taaatttaga taaaatattt gacgaatacg acgatgtttt caaagctttg gtggaaaaat
      481 gacaatctat ctgacagaaa agcaaattga aaaaataaat gctttagcaa ttcaacggta
      541 ttctccaaat gagaaaattc aaacagttag ttcttctgcc ttaaatatga ttgtgaactt
      601 accagaacaa tttgtctttg ggaagtctct ttatccaaca atttttgata aagcaacgat
      661 actatttgtc caattgataa agaagcatgt ttttgctaat gctaataaaa gaactgcttt
      721 cttcgttttg gtcaaatttt tacaattaaa cggctatcgt ttttctgtaa cggtagaaga
      781 agcagtaaaa atgtgtgtaa ccatcgcagt agaagcttta actgatgaaa aaatgacaag
      841 ctactccaaa tgggtttctg aacattctgt tagagaaaag gtcaaaaagt aacctagtat
      901 gctggatttg aatgagcaca agaaaataaa tgaacagaca atattagaat tctgtaatgc
      961 agaaactgat attgtctctt tttattgatg aataagaaag tgagaaatta tggaatcaaa
     1021 agttacaatt atcatgcaag aaatgttacc tcttttaaat aatgaacaat tactagcgtt
     1081 gagagagagt ttagaacatc atctagtaga cggaaaaaag cagcagaagt attcgaataa
     1141 taacctgttg caactattta ttaccgccaa gcaggtagag ggctgtagct caaaaacaat
     1201 tcgttattac cagaggacga ttgaaaactt gtttaatgct attaaagagt ctgtgacaca
     1261 actcacaaca gatgatttaa ggagttattt agcaaattac cagtctgaaa aggattgtag
     1321 taaggcaaat ttagacaata ttaggcgtat attgtcttct ttttttgctt ggcttgagca
     1381 agaggaatat atcattaaaa atcccattcg acggataaag aaaattaaga ctgagcaaaa
     1441 tgtgaaggaa acttatactg atgaacattt ggaaattatg cgtgataact gtgaaaattt
     1501 gagagatttg gcaataatag acctactagt atcgacaggt atgcgtgtag gggagcttgt
     1561 acagttgaat cgttcagata ttgattttga aaacagagag tgtgttgtct ttggtaaagg
     1621 aaagaaggag agaccagtat attttgacgc tcggacgaaa attcatttaa gaaattatct
     1681 taacgacaga aaagatagtc accctgctct ttttgtaacg ctagttggaa aagcccagag
     1741 acttggaatt gctggtgtag agattcgctt aagaaagtta ggagacaaac tcggcataca
     1801 aaaggttcac ccacataagt tcagaagaac tttagcgact aaggcaattg ataaaggtat
     1861 gcctatcgaa caagtccaaa aactgctagg tcatagcaag attgacacaa ccttggccta
     1921 tgccatggtc aatcaaaata atgtcaagca ttcacaccaa aaattcatct cttaaaagca
     1981 aattcccgat ttaacgagat gtttggggat cctttaaata ataacaagaa atttgcagta
     2041 aaaacgggac aacagtgttt taaattttca agtggaaaat ttcttgataa acatgataga
     2101 gtttttgaag gttatcctgc ctatggtgga aatggcattg cttggaaaag tcgaaaatat
     2161 ttaattgata atcctactat catcattggt cgtgtaggtg catattgtgg taatgtaaga
     2221 actacccacg gtaaggtatg gataagtgac aatgctatat atataaaaga atttaagaat
     2281 tccgatttca atcttgtatt tttattagaa ttaatgaaag taattgattt tagtaaattt
     2341 gctgatttct caggtcaacc aaagataact caaaaaccat tagaaaatca gaaatatatc
     2401 ctcccccctc tagccctcca aaatgagttc gcagactttg tagccctggt cgacaaatca
     2461 caatttgctt gtgagatagc tatataaaag tgtggagaaa tagcttgaaa tttagtataa
     2521 tatagctaaa ctatttgttt aaagtgagaa aaaaatggga aattttagct ttcttttaaa
     2581 aaatgacgaa tatgaatctt tttcaaaacc ttgcattgaa gctgagaata tgattgctac
     2641 atcaactgtg gctactgcct ttatggcgcg tcgtgcttta gagcaggctg tccattggat
     2701 atatagtcac gattcatatt tagaagctcc ctatcgtgct actctatctt ctttagtatg
     2761 ggatgatgat tttagggata tcgtagattc tgaactccac aagcagatag ttctgttgat
     2821 tcggtgggga aaccatgctg ctcatggtgg tgaaattaag gaacgagaag cgattttagc
     2881 tttgcatcat ttgtatcagt ttgttaattt tatcgattat tgttacagca atgagtttgt
     2941 ggagcgttat tttgatgaga agtgcttacc actttcagca aacatcaaat accgagaaac
     3001 tccacaatct atgataaagt tacaagacag tttaccagaa ctgcctgatt ttcatgaaca
     3061 gatggctgct cagtccgtag aagttcaaga gacttatact gaaaaacgtg agactgcagc
     3121 gcaacggcaa gatgtgcctt tccatattga tcaattatct gaggcagaga caagaaagct
     3181 ctttattgat atcgatctcc gtttagcagg atggatattt ggagaaaact gttgtgttga
     3241 gatagccgtt gatggtctca agcacggttc aggaattggt tactgtgact atgtacttta
     3301 tggtaaaaat gggaaaattt tagcgattgt ggaggctaaa aaagcctctg tcaatccaga
     3361 agtaggggaa gtacaggtca aagaatatgc tgaagctttg gagaaacata tcggctatca
     3421 gccaatttgc tttattacaa atgggttgaa gcactatata cttgatggtc cgaaccgacg
     3481 ccagattgca ggcttttact ctcaagaaga attgcaatta gtgatggata gacgtcatct
     3541 tcaaaaacca cttgaggata tttctagtaa aattagggac gatatttccg ggcgtcacta
     3601 ccaaaaacat gccattgcaa gcgtttgtga agctttctct aatcatcgta gacaggcact
     3661 tttggttatg gcaactgggg cggggaaaac tcgtacagca gtttctctag ttgatatctt
     3721 atcacgtcat aactgggtaa aaaacgttct cttcttagcc gatagaactt ccttggttaa
     3781 gcaagcctat gattcgttta gaaaattact cccagatctt tccgtttgta acttcttaga
     3841 agataaagaa ggagctcaat caagtcgtat ggtcttttca acttatccga ccatgattgg
     3901 agcgattagt ggtcaagaag aagtaaatca acgccctttt actgttgggc attttgacct
     3961 tatcataatt gacgaatctc accgttctat ttatcagaaa tacaagtcca tttttgatta
     4021 ttttgatgca agaattgtag gcttaacagc tactccgcgt caagatttag ataaaaacac
     4081 ctatggattc tttaatttgg agaatggggt tccaacatat gcatatgatt tggaagaggc
     4141 tgttaaagac ggatatttag tagcctatca ttctatcgaa accaaactga aactacctac
     4201 ggatggtcta cattatgatg atttgtccga agaagaaaag gaacattttg atagcaaatt
     4261 tgaagacgat agctgtgaaa aagatattga tgggagtgta tttaattcct ttgttttcaa
     4321 taaaagtaca gtagaaattg ttttaaatga actcatgaca agaggaattc agacagcctc
     4381 gggtgatgaa attggtaaaa ctattatttt tgctaaaaat catgatcatg cagaatatat
     4441 cagaggtatt tttaacaacc gctatcctga aaaagggagc gactatgctc aggtgattga
     4501 ttatagtatt aagcattatc agaccttgat tgatgatttt aaaattgagg agaagtatcc
     4561 tcaaattgcg atttctgtcg atatgttaga tacaggtatt gatgtaccag aggttgttaa
     4621 tttagtcttc ttcaagaaag tacgctctaa aactaagttt tggcagatga ttggtcgagg
     4681 aacccgtcta tgtaaagatt tatttggacc tgagcaggat aaggaaaact tcttggtatt
     4741 tgattatggg gataattttg attattttcg tgcagatcca agagatggag agggtcgtca
     4801 cattgtttcg ttgactcagc gtttatttaa tatcaaagtg gacttgattc gagaacttca
     4861 gggactccaa taccaagaag atcagtttgc gagagcatac cgtcagcagc ttgtctcgga
     4921 acttcaaggt cgtatagaga gcttaaatga gttggacttc agggttcgta tggttttaga
     4981 tacagtttat agctatagga aattggaaag ttggcagaat ctaactgctg ttacaagtga
     5041 aaccattcaa aaaaatctct ctccgctttt atttgatgaa gataaagaag atgagatggc
     5101 caggagattt gatttgtggt tgcttcatat tcagttgggg caactgacag ctaaatcttc
     5161 cactgttcat atttcccaag tgatgaagac ggctagagct ctttctgcta ttggcaatat
     5221 cccgcaggtt tttgagcagg ctgaaattat caggaaagta caggagcctg aattttggaa
     5281 agaagttaac ttgtctgatt tggaaaaaat tcgtcttgct attcgagatt tattacagtt
     5341 tttggataaa acagaccgta aaccctacta tgttaacttt gaagatcgta tactctccac
     5401 tgttcacgag accacagcat ttttgcaggt caacgatctt cggtcttaca atgaaaaagt
     5461 tgagcattat ttgaaaactc atctggatga ggagtccatt tctaagctat accataataa
     5521 aaagttgaca tctgatgata tgcttgcact tgaaaaattg ctttgggaaa aattaggtag
     5581 taaagcagac taccaaagtc attatgaaaa taaggcaatt ccgagattgg ttcgtgagat
     5641 tattggctta gatagagagt ctgccaatcg tattttttct aaatttttgt cggatgagaa
     5701 tcttaatgcc aggcagattt catttgtaaa attgattgta gactacattg tagaaaatgg
     5761 ttttttagag acgaaagtgt taacgcaaga gccgtttaaa tcttatggtt ctgttcaact
     5821 actcttccaa caccaactac cagtacttcg taatattgtt caaatcattg aacttatcaa
     5881 taatcgagct ggagaagcgg cttaaattct aaagtgattg ccatgctgag actcatttaa
     5941 aatt
//
LOCUS       DAVLYR010000111         5806 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLYR010000111 DAVLYR010000000
VERSION     DAVLYR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.111"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            2..268
                     /locus_tag="VYO63_001578"
     CDS             2..268
                     /locus_tag="VYO63_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1672470.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            261..629
                     /locus_tag="VYO63_001579"
     CDS             261..629
                     /locus_tag="VYO63_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1672471.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGVVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            749..2017
                     /locus_tag="VYO63_001580"
     CDS             749..2017
                     /locus_tag="VYO63_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1672472.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKISFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2014..3291
                     /gene="tilS"
                     /locus_tag="VYO63_001581"
     CDS             2014..3291
                     /gene="tilS"
                     /locus_tag="VYO63_001581"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1672473.1"
                     /translation="MREQDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNIDVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3295..3837
                     /gene="hpt"
                     /locus_tag="VYO63_001582"
     CDS             3295..3837
                     /gene="hpt"
                     /locus_tag="VYO63_001582"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1672474.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            3853..>5806
                     /gene="ftsH"
                     /locus_tag="VYO63_001583"
     CDS             3853..>5806
                     /gene="ftsH"
                     /locus_tag="VYO63_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1672475.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         2025 a          864 c         1186 g         1731 t
ORIGIN      
        1 gatgagatta gataaatatt taaaagtatc gcgaattatc aagcgtcgta cagtcgcaaa
       61 ggaagtagca gataaaggta gaatcaaggt taatggaatc ttggccaaaa gttcaacgga
      121 cttgaaagtt aatgaccaag ttgaaattcg ctttggcaat aagttgctgc ttgtaaaagt
      181 actagagatg aaagatagta caaaaaaaga agatgcagca ggaatgtatg aaattatcag
      241 tgaaacacgg gtagaagaaa atgtctaaaa atattgtaca attgaataat tcttttattc
      301 aaaatgaata ccaacgtcgt cgctacctga tgaaagaacg acaaaaacgg aatcgtttta
      361 tgggagtggt attgattttg attatgctat tatttatctt gccaactttt aatttagcgc
      421 agagttatca gcaattactc caaagacgtc agcaattagc agacttgcaa actcagtatc
      481 aaactttgag tgatgaaaag gataaggaga cagcatttgc taccaagttg aaagatgaag
      541 attatgctgc taaatataca cgagcgaagt actattattc taagtcgagg gaaaaagttt
      601 atacgattcc tgacttgctt caaaggtgat aaaatggaaa atttattaga cgtaatagag
      661 caatttttga gtttgtcaga tgaaaagctg gaagaattgg ctgataaaaa tcaattattg
      721 cgtttacaag aagaaaagga aaggaagaat gcgtaaattc ttaattattt tgttgctacc
      781 aagttttttg accatttcaa aagtcgttag cacagaaaaa gaagtcgtct atacttcgaa
      841 agaaatttat tacctttcac aatctgactt tggtatttat tttagagaaa aattaagttc
      901 tcccatggtt tatggagagg ttcctgttta tgcgaatgaa gatttagtag tggaatctgg
      961 gaaattgact cccaaaataa gttttcaaat aaccgagtgg cgcttaaata aacaaggaat
     1021 tccagtattt aagctatcaa atcatcaatt tatagctgcg gacaaacgat ttttatatga
     1081 tcaatcagag gtaactccaa caataaaaaa agtatggtta gaatctgact ttaaactgta
     1141 caatagtcct tatgatttaa aagaagtgaa atcatcctta tcagcttatt cgcaagtatc
     1201 aatcgacaag accatgtttg tagaaggaag agaatttcta catattgatc aggctggatg
     1261 ggtagctaaa gaatcaactt ctgaagaaga taatcggatg agtaaagttc aagaaatgtt
     1321 atctgaaaaa tatcagaaag attctttctc tatttatgtt aagcaactga ctactggaaa
     1381 agaagctggt atcaatcaag atgaaaagat gtatgcagcc agcgttttga aactctccta
     1441 tctctattat acgcaagaaa aaataaatga gggtctttat cagttagata cgactgtaaa
     1501 atacgtatct gcagtcaatg attttccagg ttcttataaa ccagagggaa gtggtagtct
     1561 tcctaaaaaa gaagataata aagaatattc tttaaaggat ttaattacga aagtatcaaa
     1621 agaatctgat aatgtagctc ataatctatt gggatattac atttcaaacc aatctgatgc
     1681 cacattcaaa tccaaaatgt ctgccattat gggagatgat tgggatccaa aagaaaaatt
     1741 gatttcttct aagatggccg ggaagtttat ggaagctatt tataatcaaa atggatttgt
     1801 gctagagtct ttgactaaaa cagattttga tagtcagcga attgccaaag gtgtttctgt
     1861 taaagtagct cataaaattg gagatgcgga tgaatttaag catgatacgg gtgttgtcta
     1921 tgcagattct ccatttattc tttctatttt cactaagaat tctgattatg atacgatttc
     1981 taagatagcc aaggatgttt atgaggttct aaaatgaggg aacaagattt tttaaatcat
     2041 tttctcaaga agggatattt caaaaagcat gctaaggcgg ttctagctct ttctggtgga
     2101 ttagattcta tgtttctatt taaggtattg tctacttatc aaaaagagtt agagattgaa
     2161 ttgattctgg ctcatgtgaa tcataagcag agaattgaat cagattggga agaaaaggaa
     2221 ttaaggaagt tggctgctga agcagaactt cctatttata tcagcaattt ttcaggagaa
     2281 ttttcagaag cgcgtgcacg aaattttcgt tatgattttt ttcaagaggt catgaaaaag
     2341 acaggtgcga cagctttagt cactgcccac catgctgatg atcaggtgga aacgattttg
     2401 atgcgcttga ttcgaggaac tcgcttgcgc tatctatcag gaattaagga gaagcaagta
     2461 gtcggagaga tagaaatcat tcgtcccttc ttgcattttc agaaaaaaga ctttccatca
     2521 atttttcact ttgaagatac atcaaatcag gagaatcatt attttcgaaa tcgtattcga
     2581 aactcttact taccagaatt ggaaaaagaa aatcctcgat ttagggatgc aatcttaggc
     2641 atcggcaatg aaattttaga ttatgatttg gcaatagctg aattatctaa caatattgat
     2701 gtggaaaatt tacagcagtt attttcttac tctgagtcta cacaaagagt tttacttcaa
     2761 acttatctga atcgttttcc agatttgaat cttacaaaag ctcagtttgc tgaagttcag
     2821 cagattttaa aatctaaaag ccagtatcgt catccgatta aaaatggcta tgaattgata
     2881 aaagagtacc aacagtttca gatttgtaaa atcagtccgc aggctgatga aaaggaagat
     2941 gaacttgtgt tacactatca aaatcaggta gcttatcaag gatatttatt ttcttttgga
     3001 cttccattag aaggtgaatt aattcaacaa atacctgttt cacgtgaaac atccatacac
     3061 attcgtcatc gaaaaacagg agatgttttg attcaaaatg ggcatagaaa aaaactcaga
     3121 cgtttattta ttgatttgaa aatccctatg gaaaagagaa actctgctct tattattgag
     3181 caatttggtg aaattgtctc aattttggga attgcgacca ataatttgag taaaaaaacg
     3241 aaaaatgata taatgaacac tgtactttat atagaaaaaa tagataggta aaaaatgtta
     3301 gaaaacgata ttaaaaaagt cctcgtttca cacgatgaaa ttacagaagc agctaaaaaa
     3361 ctaggtgctc aattaactaa agactatgca ggaaaaaatc caatcttagt tgggatttta
     3421 aaaggatcta ttccttttat ggctgaattg gtcaaacata ttgatacaca tattgaaatg
     3481 gacttcatga tggtttctag ctaccatggt ggaacagcaa gtagtggtgt tatcaatatt
     3541 aaacaagatg tgactcaaga tatcaaagga agacatgttc tatttgtaga agatatcatt
     3601 gatacaggtc aaactttgaa gaatttgcga gatatgttta aagaaagaga agcagcttct
     3661 gttaaaattg caaccttgtt ggataaacca gaaggacgtg ttgtagaaat tgaggcagac
     3721 tatacctgct ttactatccc aaatgagttt gtagtaggtt atggtttaga ctacaaagaa
     3781 aattatcgta atcttcctta tattggagta ttgaaagagg aagtgtattc aaattagaaa
     3841 gaataatctt taatgaaaaa acaaaataat ggtttaatta aaaatccttt tctatggtta
     3901 ttatttatct ttttccttgt gacaggattc cagtatttct attctgggaa taactcagga
     3961 ggaagtcagc aaatcaacta tactgagttg gtacaagaaa ttaccgatgg taatgtaaaa
     4021 gaattaactt accaaccaaa tggtagtgtt atcgaagttt ctggtgtcta taaaaatcct
     4081 aaaacaagta aagaaggaac aggtattcag tttttcacgc catctgttac taaggtagag
     4141 aaatttacca gcactattct tcctgcagat actaccgtat cagaattgca aaaacttgct
     4201 actgaccata aagcagaagt aactgttaag catgaaagtt caagtggtat atggattaat
     4261 ctactcgtat ccattgtgcc atttggaatt ctattcttct tcctattctc tatgatggga
     4321 aatatgggag gaggcaatgg ccgtaatcca atgagttttg gacgtagtaa ggctaaagca
     4381 gcaaataaag aagatattaa agtaagattt tcagatgttg ctggagctga ggaagaaaaa
     4441 caagaactag ttgaagttgt tgagttctta aaagatccaa aacgattcac aaaacttgga
     4501 gcccgtattc cagcaggtgt tcttttggag ggacctccgg ggacaggtaa gactttgctt
     4561 gctaaggcag tcgctggaga agcaggtgtt ccattcttta gtatctcagg ttctgacttt
     4621 gtagaaatgt ttgtcggagt tggagctagt cgtgttcgct ctctttttga ggatgccaaa
     4681 aaagcagcac cagctatcat ctttatcgat gaaattgatg ctgttggacg tcaacgtgga
     4741 gttggtctcg gcggaggtaa tgacgaacgt gaacaaacct tgaaccaact tttgattgag
     4801 atggatggtt ttgagggaaa tgaagggatt atcgtcatcg ctgcgacaaa ccgttcagat
     4861 gtacttgatc ctgccctttt gcgtccagga cgttttgata gaaaagtatt ggttggccgt
     4921 cctgatgtta aaggtcgtga agcaatcttg aaagttcacg ctaagaacaa gcctttagca
     4981 gaagatgttg atttgaaatt agtggctcaa caaactccag gctttgttgg tgctgattta
     5041 gagaatgtct tgaatgaagc agctttagtt gctgctcgtc gcaataaatc gataattgat
     5101 gcttcagata ttgatgaagc agaagataga gttattgctg gaccttctaa gaaagataag
     5161 acagtttcac aaaaagaacg agaattggtt gcttaccatg aggcaggaca taccattgtt
     5221 ggtctagtct tgtcgaatgc tcgcgttgtc cataaggtta caattgtacc acgcggccgt
     5281 gcaggcggat acatgattgc acttcctaaa gaggatcaaa tgcttctatc taaagaagat
     5341 atgaaagagc aattggctgg cttaatgggt ggacgtgtag ctgaagaaat tatctttaat
     5401 gtccaaacta caggagcttc aaacgacttt gaacaagcga cacaaatggc acgtgcaatg
     5461 gttacagagt acggtatgag tgaaaaactt ggcccagtac aatatgaagg aaaccatgct
     5521 atgtttggtg cacagaatcc tcaaaaatca atttcagaac aaacagctta tgaaattgat
     5581 gaagaggttc gttcattatt aaatgaggca cgaaataaag ctgctgaaat tattcagtca
     5641 aatcgtgaaa ctcacaagtt aattgcagaa gcattattga aatacgaaac attggatagt
     5701 acacaaatta aagctcttta cgaaacagga aagatgcctg aagcagtaga agaggaatct
     5761 catgcactat cctatgatga agtaaagtca aaaatgaatg acgaaa
//
LOCUS       DAVLYR010000112         5774 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLYR010000112 DAVLYR010000000
VERSION     DAVLYR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.112"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            10..963
                     /locus_tag="VYO63_001584"
     CDS             10..963
                     /locus_tag="VYO63_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1672476.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1040..3028
                     /gene="uvrB"
                     /locus_tag="VYO63_001585"
     CDS             1040..3028
                     /gene="uvrB"
                     /locus_tag="VYO63_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1672477.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3038..3601
                     /locus_tag="VYO63_001586"
     CDS             3038..3601
                     /locus_tag="VYO63_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672478.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3567..4055
                     /locus_tag="VYO63_001587"
     CDS             3567..4055
                     /locus_tag="VYO63_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672479.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            4129..4596
                     /locus_tag="VYO63_001588"
     CDS             4129..4596
                     /locus_tag="VYO63_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1672480.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(4593..5108)
                     /locus_tag="VYO63_001589"
     CDS             complement(4593..5108)
                     /locus_tag="VYO63_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1672481.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5120..5647)
                     /locus_tag="VYO63_001590"
     CDS             complement(5120..5647)
                     /locus_tag="VYO63_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1672482.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT         1741 a         1032 c         1303 g         1698 t
ORIGIN      
        1 gacggggata tgaagaacaa aagaatattt aaagacttcc aagcttcaaa aatgagttta
       61 aacatttaca caagcccctt gttagccttt gtttttgtct tcataggaga gtttgtggct
      121 tttactttgt atggtattgg cttgttagct ctcatcggac ttgctagaaa ttttggagag
      181 gctggtcaaa atcttgcaag ctacttgcag accttgcatc agagcttgac ggataaaaca
      241 agtgactttc gtttaatttt aggattactg gcctttggtt ttattcttaa cactgtgttc
      301 agatggacaa gaaaagttga gaaaagacct attcgaacct tgggatttta tagagagaat
      361 ttcctcagca atcttctgaa aggatttagt ctaggcctgg cactttttct tctgaccttg
      421 ttaggtttag tggtcttagg tcaatatcgt ttggaatcca ttcacttgaa tccttattct
      481 cttgcctttg tcgtctttac tatcccattt tggattttac aggggacagc agaagaagtg
      541 gtggcccgtg cttggctact tcctcaattg gcctcaagaa ccaatctaaa actagctatt
      601 cttatatcta gcctgttctt taccctgctt catatgggca attctggtct cacccctcta
      661 tctctagtaa atctcttttt attcggagtt gccatggctc tttaccttct caaaactgat
      721 acagtttggg gtgttgcagg tattcatggt gcttggaatt ttgctcaggg taatctcttt
      781 gggattttag ttagtggtca accgtcagga acgtctctga tgaccttttt accacaaggc
      841 aatcaagatt ggctatcagg tggttctttt ggcatagaag gttccattat gacaagtctg
      901 gtattactac tgctgattgt ctatcttgct aataaattaa agaaagaaaa tgaaaggatg
      961 tgacttcggt ccgtcctttt cttcgtgaaa atactataag tatgctaaaa taggaatagc
     1021 acatggagag aggattctta tgatcaatca cattacagat aatcaattta aactagtatc
     1081 aaaatatcaa ccatcaggag atcaacccca agctatcgag cagttggtgg ataacattga
     1141 ggggggagaa aaagctcaga ttctgatggg ggcgactgga acagggaaga cctatactat
     1201 gagtcaggtc atttctaaag tcaataaacc aactctggtt attgcccaca ataaaactct
     1261 ggctggtcag ctctatgggg agtttaagga atttttccct gaaaatgcag ttgagtattt
     1321 cgtatcctac tatgattatt accagccaga ggcctatgtc ccttctagcg atacctatat
     1381 tgagaaggat agttctgtca atgacgagat tgacaagctt cgccactcag ctacctcagc
     1441 ccttttggag cgtaatgatg ttattgtcgt ggcctcagtc tcttgtatct atggtttggg
     1501 ttcgcccaag gaatacgctg atagtgtcgt tagtctccgt cctggtctag agatttctcg
     1561 tgataaactc ttgaatgact tggtcgatat tcagtttgaa cgtaatgata ttgatttcca
     1621 acgcggaaga tttcgcgttc gtggggatgt ggtagagatt ttcccagctt cccgagatga
     1681 acatgccttt cgagtagaat tttttggaga cgaaattgac cgtattcgtg aagttgaggc
     1741 tctgacaggt caggtgttgg gagaagtgga tcatttagcg attttcccag cgacacactt
     1801 tgtgaccaat gacgaccaca tggaagttgc cattgcaaag attcaggccg agttggaaga
     1861 acaattagct gtctttgaaa aggaaggtaa actgcttgaa gcccagcgtt tgaaacagcg
     1921 gacagagtat gatatcgaaa tgttgcgtga gatgggctat accaatgggg ttgaaaatta
     1981 ttctcgccac atggatggac ggagcgaagg agagcctcct tatacgcttc tcgacttctt
     2041 cccagatgat ttcttgatta tgattgacga gagtcatatg accatagggc aaatcaaggg
     2101 catgtacaat ggagaccgtt cgcgtaaaga aatgctggtt aattatggtt tccgtttgcc
     2161 gtctgctttg gacaatcgtc ctctccgtcg ggaggagttt gagagtcacg ttcatcagat
     2221 tgtttacgtt tcagcgacac ctggtgacta tgaaaatgaa cagaccgaga cagtgattga
     2281 gcaaatcatt cgtccaacgg gactcttgga tccagaggtg gaagtccgtc cgactatggg
     2341 acagattgat gacctcttgg gtgaaatcaa tgcccgcgtt gaaaaaaatg agcgtacctt
     2401 tatcacaact ttgaccaaga aaatggcaga ggatttgacc gactacttca aggaaatggg
     2461 tatcaaggtc aagtacatgc actcggatat caagaccttg gaacggacgg agattatccg
     2521 tgacctgcgc ttgggtgtct ttgatgtctt ggtcggaatt aacctgctcc gtgaaggaat
     2581 tgacgttcct gaagtgagcc tcgtagctat tctcgatgct gacaaggaag gtttccttcg
     2641 caacgaacgt ggactcatcc agactattgg acgtgctgct cgtaacagtg aaggccatgt
     2701 catcatgtat gcagacacgg ttacccagtc tatgcaacgt gctatcgatg aaactgcccg
     2761 ccgtcggaaa attcagatgg cctataatga agagcatggt atcgtaccac agacaatcaa
     2821 gaaagaaatc cgtgacctga ttgccgtgac caaggcagtt gctaaggaag aggacaagga
     2881 agtcgacatc aatagcctca acaaacaaga gcgcaaagaa ctcgtcaaga aactagaaaa
     2941 acaaatgcaa gaagccgtcg aagtgcttga ctttgaactg gcggctcaga tccgtgatat
     3001 gatgttggaa gtcaaggcct tggattaggg gaatagtatg atttatttaa gaaagttaaa
     3061 gagagaagat ttgatgtctt tatgggaaat ggcttattca caacttaatc cagtttggaa
     3121 acagtatgat gctccctatt atgatgatta tcagtatttt tcaaatttta aagaattcga
     3181 actacaaaaa tcagaatcca ttttaagcaa ctcaaatcgc cttggtattt ttgttgatga
     3241 taaactagtt gggactgttt cgcgttattg ggtatgtaaa caaacaagat ggatggaatt
     3301 gggaattggt atttatgata aaaaattctg gaacactggt attgggaaag ttgctatgtt
     3361 gcagtggata gataggacgt ttcaggatta cttggagttg gagcatctgg gtttgacaac
     3421 ttggtcaggg aatattggta tgatgaaact tgctgaaaaa ttaagaatga aaaaagaagc
     3481 tcatattcca aaagttcgtt attatcaagg taaatatttt gatagtatta aatatggtat
     3541 tttgagagaa gactgggaga aaataaatga cggttattat caaatcaatg gaaactccta
     3601 aagagataga aggtaaatcc cttgttcact ggcaaacgtg gagagagtct tatgatgacc
     3661 ttttgcctgc ggaatttcag gatacaatga cattagaaag atgtcgattc tttagtcaaa
     3721 agtatccaga aaatacattg attgcgatgg atggcgtgaa ggtggttggt tttatcagtt
     3781 atggcaattt tcgtgatgag actattcaag ctggtgaaat tattgcttta tatgttttaa
     3841 aagactatta tggaaaaggt atcgcacaaa agttaatgaa agcagctttg actgctctta
     3901 atcatttttc tgaaattttc ttatgggtat tgaaagataa caagcgcgcc attgctttct
     3961 atcaaaaaat gggttttact tttgatggac aagaaaaaat acttgaactt ggaaagccta
     4021 taaaggaaaa acgaatggtg ttctattcta aataatttta aaaaagtaaa agataatatc
     4081 tctggtagta agtctgaaaa tttaataaat tagaaagcga gtaaatttat gtcccgttcc
     4141 caattaacaa ttttaacaaa tatctgtctg attgaagacc tcgaaactca gcgcgtggtg
     4201 atgcagtatc gcgcccctga aaacaatcgc tggtctggtt atgcctttcc tggaggtcat
     4261 gtagaaaatg atgaggcttt tgcggagtct gtcattcgtg aaatctacga agaaacaggg
     4321 ttgactatcc aaaatcctca acttgtcggc attaaaaatt ggccactaga tacaggtggg
     4381 cgctatattg tcatttgtta taaggcgact gagttctctg gtacccttca atcttcagaa
     4441 gagggagaag tttcttgggt gcaaaaagac cagattccaa acttaaatct ggcctatgat
     4501 atgctaccat tgatggaaat gatggaagct cccgacaagt cagagttttt ctatcctcgc
     4561 cgtacagaag acgattggga aaagaaaatc ttctagtctt ttactaaata acctagctga
     4621 tccaaggcct cctcgatata gtggaggtct tgttgtgttt cggcttcgac taggtggtaa
     4681 tggatgccat ctgtcaattc agatagaggt ctaaagtcag aatgctcgac ttgttctaga
     4741 aaatgttgca catctctgcg acaagacagt ttaagcaagg tttcaatttc tccataaacg
     4801 ggatggtcaa tcaaggtatt ctggacatgc ccaccattat cgacaatagc aaggagttcc
     4861 tgaccgattt cttcaacttc atgtttcact ttgaaaagtt tgtgaacata aggattggta
     4921 tcaatttgct tatagatgta gccacgattg gtggatagga taggagcgcc atcggctctc
     4981 aaaattgcaa tgtcctgcac aatgacctga cgtgtgacat gaaaatgttc cgccaaactt
     5041 tggccattaa gggctttagg agcttctttc aagagttgga gcagggcttg tttgcgatct
     5101 tttgtcatag cttttccttt taacggcgtt ttcgaagcac tttatagaca gctagtgcta
     5161 atgtatagtc taccatacta tggataattg tgccaaatcc aactagtaca aatagaacat
     5221 agaacatatt ttctacattg gtacccgaag ttgcgtaaaa aaggacacag gctaatactt
     5281 cagcaagggc atgaacaaca gccaaaacaa aattgaaaac ccaggaagat tttggtttat
     5341 ctagggtatc ggggaatttt tgtaggtaaa gagctcctaa agcaccaaaa gatatatggg
     5401 aaaaagcccg aaaaacgata accatgggat agccagccat caaaaatcca aaactagagg
     5461 ctaggatgac aaaaactgcc atcaagggcg acaagaacat ggctataaaa atagcgatgt
     5521 ggctccccaa agtataggaa gcaggtggaa tgacaatctt gaaaggcata acaattggaa
     5581 tcaaaatcgc aatagccgtt aaaagggctg tcattgtcat aaattgtgtc tttttccgtg
     5641 tattcacaag aatctccttt ttaactgcat atacactagt atggtacaat aaaccagaca
     5701 ataaagcaag aatttacttg ggtttataga tcatttttta gttaaaagtt atagtagatt
     5761 gaaactagaa tagt
//
LOCUS       DAVLYR010000113         5746 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLYR010000113 DAVLYR010000000
VERSION     DAVLYR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.113"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            162..683
                     /locus_tag="VYO63_001591"
                     /pseudo
     CDS             162..683
                     /locus_tag="VYO63_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(676..843)
                     /locus_tag="VYO63_001592"
     CDS             complement(676..843)
                     /locus_tag="VYO63_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1672483.1"
                     /translation="MKKRILSAGLTFTSALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(1008..2615)
                     /locus_tag="VYO63_001593"
     CDS             complement(1008..2615)
                     /locus_tag="VYO63_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1672484.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDELIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2768..4252
                     /locus_tag="VYO63_001594"
     CDS             2768..4252
                     /locus_tag="VYO63_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1672485.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4347..4829
                     /locus_tag="VYO63_001595"
     CDS             4347..4829
                     /locus_tag="VYO63_001595"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1672486.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(5116..5337)
                     /locus_tag="VYO63_001596"
                     /pseudo
     CDS             complement(5116..5337)
                     /locus_tag="VYO63_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1689 a         1286 c         1073 g         1698 t
ORIGIN      
        1 atttttttta ctatctgcat ctttaagtat cttagtagac ttcccgcgaa acaaaaatat
       61 agtaaaatga aataagaaca tgacaaatcg atcaggacag tcaaatcgat ttctaacaat
      121 gttttagaag cagaggtcta ctactctagt ttcaatctaa ttatgaacaa gacccagaaa
      181 aagtagcctt atttcttaag aattttaata gtttaaagca cctagcacct gtttagattg
      241 atgaaacagg attcgatact tatttttatc gagaatatgg tcgctcatta aaaggtcagt
      301 taagaagagg caaagtatct ggaagaagat atcagaggat ttctttggtt gcaggtctaa
      361 caaatggtga attaatcgct ccaatgactt acgaagagat gatgacgagc gacttttttg
      421 aagtatggtt tcagaagttt ttcttaccaa cattaaccac accatcggtt attattatgg
      481 ataatgcaag attccataga atggggaagc tagaactttt atgcgaagag tttgggcata
      541 aacttttacc tcttcctccc tactcacctg agtacaatcc tattgagaaa acatgggctc
      601 atatcaaaaa gcacctcaaa aaggtattac caagttgcaa taccttttat gaggctcttt
      661 tgtcttgttc ttgtttcaat tgactatatt atcagcgtaa tagtctaata gatagttaaa
      721 tgtagttgga tttccactaa aggttgatga gtaagttttt gtatctgaac ctgattggtc
      781 gcaagcagct aaaagcaaag cagatgtaaa agtcaggcct gcactaagga tacgcttctt
      841 tatgttcgtc ttctttctcc ttaatagtgg gaatttgtaa agttaattga atttcgagaa
      901 tgaaggttta taaaccttgg ttataaaaaa caaaggattt ctgcctttta tacagtcctc
      961 ccttgttttt atacgatttc aattttaaat ttttctgcaa aaaatattta tagtaattcc
     1021 acacagaaag catcccatgg aactaagatt tgtttttcaa agacttcttg agctagggtg
     1081 ttttcaatca agacagattt gacttttcct tctactgtca agtcttgctc ttcattggac
     1141 aagttagcca caactaggaa gcgacggtcg ccatccttac gtatataagc aaagacctta
     1201 tcagccgtat caagcaattc aaagtcagct cgaattagcc agctattctc cttgcgaatt
     1261 tggaccagtt tctgataggt atagaaaata gaatctggat ttgccagcgc ttcttggacg
     1321 ttgatcatct cgtaatttgg attaactgcc aaccaaggtt gacctgttga gaaaccagcg
     1381 tttttgctct cgtcccattg cataggggta cgggcattgt cacgtccaat aacacggata
     1441 ctgtccatga tttcttcaat cggaacacct ttttcaagag cctcacgcgc atagttgaga
     1501 gattcaatat cttctacttg atccagtgtt tcaaacggat agttggtcat cccaatctcc
     1561 tcaccttggt agatataagg agttcctctc atgagatgaa gtaagattgc aaaggctttg
     1621 gcagattttt cgcggtattc ttggtcattt ccccagattg agacaatacg agggaggtca
     1681 tggttgttcc agaagaggga attccagccg tcctcaactc ctaactctgt ctgccatttg
     1741 ttgaagattt cttttaactt agcgatattc agctcttttt gatagtgcca tttaggttga
     1801 ccttcctgat actgaagacc gatatgttca aactggaaga ccatagacaa ttcttgcccc
     1861 tttggatcag agtagagttt ggcaatctct ggcgttgctc cccaagtctc ccctactgtc
     1921 aagagatcct tatctccaaa ggtcgcctga ttcatttcct tgagataggg atggagcata
     1981 ggaccattat tgactacctt ctcgtcagga attttgccaa tcatgtcaat aacatccata
     2041 cggaaaccac caataccctt atcaatccag aagttcatca tctcataaat tttctggcga
     2101 agtttttcat tttcccagtt gagatccggc tgtttcttgc tgaaaaagtg gagatagtat
     2161 tgacctgact tttcatcgta ttcccaagca gacccactaa agatagaatc taaatcgtta
     2221 ggctcatccc gccagatata gtagtctcgc tcagggctat taggattttc acaggcctct
     2281 acaaaccagg catgctcatc cgaggtatga ttgaccacca agtccatgat gatacggata
     2341 tcacgcttct tagcttctgc aatcagttca tccatgtcct ccatggttcc aaaaatagcc
     2401 gcaatcgctt gataatcagc aatatcatag ccattatcat ccatagggct gtcataaacg
     2461 ggagaaagcc aaatcgctgt gattcctagc ttagctagat agtccaactt actggtaata
     2521 cctggcaaat caccaactcc atctccatta ctatccataa aactctttgg atagacttga
     2581 tagactacgg cattatgcca ccatttttct tgcatctgtt tacctcatta ttttaataat
     2641 ctatagtata tgatagcact ttttaaaatt ttgtgcaagc gtttgcttaa tctttttaat
     2701 ttctttttta gctccgtagt ttcctaactt ccaatttttt attataatag aatccagagg
     2761 taaaaaaatg aaaaaacaag cttttagttc tgaacagtat ttgaatttac aacgcgacca
     2821 catcttagag cgcattaacc aatttgacgg caagctctac ttagagtttg gcggtaaaat
     2881 gttagaagat ttccacgctg ctcgtgtcct tcctggttat gaacctgaca acaaaatcaa
     2941 gctcttgcaa gaattgaaag agcaggttga ggttgtgatt gccattaatg ctagcaacat
     3001 tgaacattcc aaagcacgtg gcgacttagg catttcttat gaccaagaag ttcttcgttt
     3061 gattgacaaa ttcaatgaat tagggatttt tgttggttcc gttgtcatta cacaatacgc
     3121 tggccaactc gctgcagatg ccttccgcaa ccaacttgag aaaaacggaa ttgattctta
     3181 tcttcattat ccaatcaaag gatatccgac ggatatggat cacatcattt ccccagaagg
     3241 catgggcaaa aacgactaca tcaaaaccag tcgcaacttg attgtcgtaa ccgctcctgg
     3301 acccggttct ggaaaattgg caacgtgtat gtccaatatg taccacgacc aaatcaatgg
     3361 catcaagtct ggctacgcta aatttgaaac cttccctgtt tggaatcttc cccttcatca
     3421 tccagttaac ttggcttatg aagctgccac agctgacctt gatgatgtca acatgattga
     3481 ccccttccat cttcaaacct atggagaaac cactgtcaac tacaaccgtg atatcgaaat
     3541 cttcccagtg ctcaagcgca tgttggaacg tattctcgga aaatcaccat acgcttcacc
     3601 gacagatatg ggtgtcaaca tggttggttt cgctattaca gatgacgagg ctgctgtcga
     3661 agcttctaaa caagaaatca tccgccgtta ctatcaaaca gttcttgact tcaaagctga
     3721 aaaagttggc gaagctgccg tcaagaaaat tgagttgctc atgaacgacc tcggtatcac
     3781 acctgcagac cgtaaggttg ctgtcgttgc gcgccaaaaa gcagaagaaa ctggtggacc
     3841 agccctagcc tttgaattgc caaatgggga aatcgtcact ggtaagaact cagaactctt
     3901 tgggcctaca gccgctgcct tgatcaacgc catcaaaaaa tcagctgaca tcgctaaaga
     3961 agtaaaacta atcgagcctg aagttgttaa gccaatccaa ggtcttaaaa tcgatcatct
     4021 cggtagccgc aatccacgcc ttcattcaaa tgaaatcctg attgcacttg ctatcacagc
     4081 tacagaaaat cctgatgctg cccgcgctat ggaagaactc ggcaacctca aaggaagcga
     4141 agcccactca accatcatct tgactgatga agacaagaat gtccttcgta aactgggtat
     4201 caacgtaacc tttgacccat actaccaata cgaccgctta tatcgtaagt aaagatttca
     4261 acctctccag cctcggagag gttttctctc tgtctcagga gcaaccttta tgatataatg
     4321 agagaagaaa actgaaagga tttaccatgt caaaagaagt tattgtcgaa agttttgaac
     4381 ttgaccacac cattgtcaaa gcaccctatg ttcgcttgat tggggaagaa acaggaccaa
     4441 aaggagacat catctccaat tatgatattc gcttggtgca accaaacgaa gactctatcc
     4501 ctactgccgg ccttcacact atcgagcacc tcttagccaa actcatccgt acccgcattg
     4561 acggcatgat tgactgttca ccatttggtt gccgcacagg cttccacatg attatgtggg
     4621 gacgtcacac cagtgctaaa atcgcagctg ttatcaagga ttcgctcaag gaaatcgctg
     4681 aaactactac ttgggaagat gtcccaggga caaccatcga atcttgcgga aactacaagg
     4741 accacagcct cttctctgct aaagaatggg caaaactcat cttggaacaa gggatttcag
     4801 atgatgcctt tgaacgtcat gtgatttaaa atcgagtagg ggataatctc tagcccctct
     4861 cacaccaccg tacgtaccgt tcggcatacg gcggttcaac taacttttta acgcatgtcg
     4921 ttcaaggtaa taatccaaac acgaaaccaa tccacgtttt tcaaggactg gttttgatat
     4981 agcacgttta agtaccgact tctgagctac tatagtggat tgaaactaga atagttcacc
     5041 tctgcttcta aaacattgtt agaaatcgat ttgactatcc tgatcgattt gttctattct
     5101 tatttcattt tactataatt gatagtggtc gccccagcca gataccttat ctgctatcca
     5161 tttaggaacc cctaacttaa gcaatcccca taatcgtctc gatttcttct tccattgctt
     5221 ccagataatc actcgtaggc gagtatgcaa acgctcatct atgctggtga ctatactttt
     5281 catatttccc aatgaaaaat agtttatcca tcctcgaatg gacaaattca gttgctctcg
     5341 tgatttacgc atataggtta cgactacctt tttggacttt agagtttgag gcccccttat
     5401 ccgctatata cgccttacta tcacgttcct gttcgtagag ccacgagttc gctatccctt
     5461 cctctgacga ttcttatttc cagcgtcaga cttaaaacgg tctatcccca gaccgtttta
     5521 atccgctacc tcacgatagt caggcttggg aatcgctatt gggttcaccg gtaacaggtg
     5581 cccaccgagg acttacacct cagatatata gtaaaatgaa ataagaacag gacaaatcga
     5641 tcaggacagt caaatcgatt tctaaaaatg ttttagaagt agatgtgtac tattctagtt
     5701 tcaacctact atacgacatg cccgtcgtac ctcaaaagga accagt
//
LOCUS       DAVLYR010000114         5707 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLYR010000114 DAVLYR010000000
VERSION     DAVLYR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.114"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(20..2491)
                     /gene="parC"
                     /locus_tag="VYO63_001597"
     CDS             complement(20..2491)
                     /gene="parC"
                     /locus_tag="VYO63_001597"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1672487.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLRIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKNFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(2911..4854)
                     /gene="parE"
                     /locus_tag="VYO63_001598"
     CDS             complement(2911..4854)
                     /gene="parE"
                     /locus_tag="VYO63_001598"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1672488.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            4990..5631
                     /gene="plsY"
                     /locus_tag="VYO63_001599"
     CDS             4990..5631
                     /gene="plsY"
                     /locus_tag="VYO63_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1672489.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         1514 a         1331 c         1123 g         1739 t
ORIGIN      
        1 aactgattct tcatttcact tatttatctt cagtaactac ttcctgaaga taagcgtcaa
       61 aaacttcttc atctgaaatc gtgtcagaaa tgaagcttcc attgctagtg cgttctgaca
      121 agttcaagtc ttgcaatcgg ctttcataga ttgttccttt attggattgg acaagcagag
      181 tttggtcgtt cacatccact tccgtactga agaaatcgcc aacaaatcct tgctctgcaa
      241 ctgctcctgc caagaagaca cgatgcggtt tgtttttcaa ctcacgcaag acttgtaatc
      301 ctcgtttggc acggctggtt gctagaattt cctcaatgga aacacgtttc aagcttccac
      361 gctgggtcaa gaggtagaag gacgaagtat tacagataaa gccagattgg aggacatcat
      421 cttctttcaa attcatagcc ttgacacctg ctgccttagc accgacaacc ggaacctctt
      481 cgatattgaa acgcagggca taaccatttt gactaaccaa gacaacatca tctagtttaa
      541 tcggagccac tgctacaatc tgatctgtat cgtctttgag cttagcatac ttgacagact
      601 tagatctata ggtccgccat ggagtgaatt cttttcgctc tacccgtttg atttgaccaa
      661 ggcgagtcgc tgcaaagtag gttgtcgcat cgtcaaactg atccagtact tccacataaa
      721 ggattgcttc attcgtttca aagtttgtga tggtttggct cagatgctct ccgatgtcct
      781 tccaacgaat atctgccaac tcatggattg gtctgtagat gacatttcca agacttgtga
      841 acatcaagag gtgctgggtt gtcttggcag attgaacaaa aatcaaacgg tcatcatcac
      901 gcttgccaat ttcttccaag gtggaagccg caaaggaacg tggactggta cgcttgatgt
      961 aacctgcctt ggtcacgctg acgtaggtat cttcctcagc gataagacta gctgtatcaa
     1021 tctcaattgc tttcgcagtg tcttctaaag aactcaaacg aggagttgca aagttcttct
     1081 tgacctcacg aagttctttc ttcatgagat tgtacatagt cctttcatca ccgataatag
     1141 ccgccagcat agcaatcttc tcacgaagct ctgcttcttc ttcctgcaag acaaccacat
     1201 cggtattggt caaacggtac agttgcaaag ttacgatagc ctcagcctgt tcttccgtaa
     1261 aatcatagct aactttgagg ttttccttgg cgtccgcctt attctcagaa gcacggataa
     1321 gagcaatgac ttcatccaaa atcgaaatca cacgaatcaa accttcgacg atacggagac
     1381 gtttctcagc cttttctttg tcaaagcgtg aacgcgccaa aatcacttct cgacggtgag
     1441 cgatgtagct agacaggatt ggaacaatcc caacctgacg aggtgtgaaa ttgtcaatcg
     1501 ccaccatatt aaagttgtag ttgatttgta ggtcggtgta cttaaataag tagttgagaa
     1561 caagctcagt attagcgtct ttcttaagtt caatagcgat acgaagacca tcacggtcag
     1621 actcatcacg aacctcagca atcccagcta ccttgttatt aacacgaaca tcatcgattt
     1681 tcttgactag attggcctta ttgatttcat aaggaatctc agtaataacg atttgttcct
     1741 taccaccttt tagcttttca atttcagtct tggaacgaac aaccacgcgc cctttcccag
     1801 tctcataggc tttcttgatt tcatcacgac cctgaataat agcccctgta gggaagtctg
     1861 gtccaggtaa gaattccatg agtttatcaa tctttgcagt tgggtggtca atcatgtaaa
     1921 ctgcagcatc tatgacctca gctaaattat ggggaggaat gtctgtggca taaccagccg
     1981 aaatcccagt cgaaccattg accaagaggt ttggaaaggc tgctggcaag accgttggtt
     2041 ctttctccgt atcgtcaaag ttccatgcaa aaggaactgt ctttttctcg atatcctgaa
     2101 gaaggtagcc tgcaatttca gacaaacgtg cctcagtata acgcatagcc gcaggaggat
     2161 ctccgtccat agaaccgtta ttaccgtgca tttcaactag aatctcacga tttttccagt
     2221 tctgtgacat acgaaccatg gcatcataga tagaagaatc cccgtgtggg tggaaattcc
     2281 ccatgatgtt cccgactgac ttggccgact tacggtagct cttgtcaaaa gtattgctat
     2341 ccttattcat agaataaaga atacggcgtt gaaccggctt caacccatca cgaatatctg
     2401 gcaaagcccg gtcttgaata atgtacttgg agtagcgacc aaagcgctct cccatgatgt
     2461 cctccaggga catgttttga atgttagaca taagatacaa agcccataaa ataccaagtg
     2521 aaaatagaaa attcttgaag taagcaaact cacaagagaa tttatctttt tcacacagta
     2581 tctagggcgt gttcaactcc tttcaaagaa tgtagagtag gtttttatgc agtaaaagat
     2641 attttacggg aattcgtccc gtgttcagtt acgataagta accaaactat cctgtttgta
     2701 tttttcaata tgaaaatctg gttttccaaa attagtctta gtttgtgtct taaccgctcc
     2761 cttaagcgcc tctttaagat aagcactcat agcagattct tcattaataa tcctgcaatt
     2821 ttttcaaacc aagattttca aactgctttt tcacatagtc attcacatcc gactctaatt
     2881 tccagtttac taacatatta ttttctttca ttaaaacact gtcgcttctt ctagcgtaaa
     2941 cttgacatta tcttcaatcc atttacggcg tggttctacc ttatctccca tgagaacatt
     3001 gacgcggcgt tcggcgcgcg ctaaatcttc aattgtgaca cggatgaggg tacgtgtttc
     3061 tgggttcatg gttgtttccc agagctggtc cgcattcatc tcaccaagtc ctttgtatcg
     3121 ttggagggta gcgcctttac cgaactgttt acggagttct tctagttctc cgtccgtcca
     3181 agcgtaggcc acttcttctt tcttgccttt acctttggac atcttgtaaa gaggtgggag
     3241 ggcaatatag acatgacctg cctcgactag cggacgcatg taacggtaga aaaatgtcaa
     3301 gagcaaggtc tggatatggg caccgtcggt atccgcatcg gtcatgataa tgatcttatc
     3361 atagttggca tcttcaatag agaagtctgc tccaacaccc gcaccaatgg tataaatcat
     3421 ggtattgatc tcttcatttt tgaggatatc cgccatcttg gccttggctg tattgataac
     3481 cttaccacga agaggtagaa tagcctggaa cttgcggtca cgaccttgtt tggcagaacc
     3541 accggcagag tccccctcaa ctagatagag ttcattctta gcaggattct tagattgggc
     3601 tggggtcaat ttcccagaca acaagccctt atctttcttg tttttcttcc catttcggct
     3661 ctcatcacgc gccttacgtg ctgcttcacg agcatcacgg gccttgatag ccttgcggat
     3721 gaggttagaa gctaattccc cattttccat aaggaaaaag gtcaacttat cagccactat
     3781 tccatccaca actgggcgag ctagggggct tcctagttta tccttggtct gtccttcaaa
     3841 ctgcaagtgt tcttcaggaa ctaagataga aagaacggcc gctagtccct cacgatagtc
     3901 tgaaccttca aggtttttat ctttttcctt gagaagacct gttttacgtg catagtcatt
     3961 catgaccttg gtaatggcag acttgagtcc tgtctcgtgc gttccaccgt ccttggtgcg
     4021 aacgttattg acaaaggata gaatgttatc tgagaatccg tcattgtact ggagggctac
     4081 ttccacttga aaaccattgt cttccccttc aaagtaaaga actggcgtca agatttcctt
     4141 atcttcgttg agataagaaa caaaatcttg tactccattc tcatagtgga actcaatcgc
     4201 ttcatctgtt cgcttgtccg ttaaagacaa ggtcacattt ttcaagagaa aggctgattc
     4261 attaaggcgc tctgaaatgg tattgtactt gaaatctgtc gtagaaaaga tagtcgcgtc
     4321 aggcataaaa gtaactttgg tgcctgtttt agacttgagt gctgtaccga ttttcttcaa
     4381 agtcgtgaca ggttttccac cattttcaaa acgttgcttg taaactgcgc catcacgggt
     4441 aatttcaact tctaaccagc tagaaagggc gttaacaacg gaagaaccca ctccgtgaag
     4501 tccacctgat gtcttatagc caccttgacc gaatttccct ccggcatgaa gaatggtaaa
     4561 gataacctca acagttggaa ttcccatagc gtgcatacct gtcggcatcc cacgtccatg
     4621 gtcttgaacc gttagactac cgtctttatt gatagttaca tcaatacgat caccaaaccc
     4681 agacaaggct tcatcgactg cattatcaac gatttcccaa actaggtgat gaagaccagc
     4741 gccatcggtc gatccaatat acatccctgg acgttttcgg accgcatcca acccttctag
     4801 cacctgaata gcatcatcat tataattgtt aatattgatt tccttttttg acacaaggaa
     4861 cctcctattc gttcatcttt actattctac aggttttcca aggattttgc aaaatttttc
     4921 tttctccgat gtgacaattt cagcagagat tctctgcttt tctttcccaa ttcatgatat
     4981 aataggagta tgattacaat agttttatta atcctagcct atctgctggg ttcgattcca
     5041 tctggtctct ggattggaca agtattcttt caaatcaatc tacgcgagca tggttctggt
     5101 aacactggaa cgaccaacac cttccgcatt ttaggtaaga aagctggtat ggcaaccttt
     5161 gtgattgact ttttcaaagg aaccctagca acgctgcttc cgattatttt tcatctacaa
     5221 ggcgtttctc ctctcatctt tggacttttg gctgttatcg gccatacctt ccctatcttt
     5281 gcaggattta aaggtggtaa ggctgtcgca accagtgctg gagtgatttt cggatttgcg
     5341 cctatcttct gtctctacct tgcgattatc ttctttggag ctctctatct tggcagtatg
     5401 atttcactgt ctagtgtcac agcatcgatc gcggctgtta tcggggttct gctctttcca
     5461 ctttttggtt ttatcctgag taactatgac tctctcttca tcgctattat cttagcactt
     5521 gctagtttga ttatcattcg tcataaggac aatatagctc gtatcaaaaa taaaactgaa
     5581 aatttggtcc cttggggatt gaacctaacc catcaagatc ctaaaaaata aaacgccagt
     5641 tctgtactgc ccccaaacag ttagacaaat aatttatcca aaggatttag ttctgtattg
     5701 cacaggg
//
LOCUS       DAVLYR010000115         5639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLYR010000115 DAVLYR010000000
VERSION     DAVLYR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.115"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(299..1147)
                     /gene="dprA"
                     /locus_tag="VYO63_001600"
     CDS             complement(299..1147)
                     /gene="dprA"
                     /locus_tag="VYO63_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1672490.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(1243..1932)
                     /locus_tag="VYO63_001601"
     CDS             complement(1243..1932)
                     /locus_tag="VYO63_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1672491.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1944..2822)
                     /locus_tag="VYO63_001602"
     CDS             complement(1944..2822)
                     /locus_tag="VYO63_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1672492.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2812..3681)
                     /gene="licA"
                     /locus_tag="VYO63_001603"
     CDS             complement(2812..3681)
                     /gene="licA"
                     /locus_tag="VYO63_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1672493.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3698..4720)
                     /locus_tag="VYO63_001604"
     CDS             complement(3698..4720)
                     /locus_tag="VYO63_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1672494.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4725..5432)
                     /locus_tag="VYO63_001605"
     CDS             complement(4725..5432)
                     /locus_tag="VYO63_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1672495.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
BASE COUNT         1787 a         1183 c          965 g         1704 t
ORIGIN      
        1 caaagcgcaa actaggaagc tagccacagg ttgctcaaag cactgctttg aggttgtaga
       61 taagactgac gaactcagct caaaatactg ttttgaggtt gcagatggaa gctgacgtag
      121 tttgaagaga ttttcgaaga gtattatatg agaaaaatcc tctactctac taatccactt
      181 agaaaaaacg catattagca agtgttaaaa acttgctaat atgcgtttta ttatggaaat
      241 gttgggttca ttttctctta aaattgattt tttcttagaa ttctagctta ggtcattttt
      301 aaaattcaaa ttccgcaaga acatcttgcc cactggtgag caattttgct ccttcttgaa
      361 tcaaatgatg gcaaccgtct gatagtccat ctaaaatgct accaggaata gcaaagacat
      421 cgcgtccttc ttccattgct cgctcacacg taatgagact acctgaacgc atcttagcct
      481 ctgctacaat cacaccacga caaagtccag caatgatgcg attacgggca ggaaaatgaa
      541 atttcagagg ttgttcgcca ggtccatatt cacttagaac cagatggtca ttgccgatgt
      601 agtcttgcaa gcgtttattg gctttaggat aaaacacatc cagtcctgtt ccaatcactg
      661 caatggtttt tccgccattc tgaagagctg ccatatgagc tgctgtgtca atgcccttgg
      721 ccagaccact gacaataacc agttcatttt ccaagccttg aatgactttt tcaactgact
      781 tagctccctg tttgctacaa gcacgactgc ccacgaccgc taccttcggg aatttcagga
      841 ggtcaagatt tcccttgtaa aataaaagta caggcgcatc atatatttca ctcaaatccc
      901 aaggataaca gtcatctaaa atagagaaag atggaaattt ttgaaactct ttcgacaaat
      961 gcgcatcgtc tatctgaaaa taacgttcca taaaaacggc tggattacgg caacctgata
     1021 tatctgcaat atcacccaac aaaagctcct gatcaacatt ttcaccgtat tctagcactt
     1081 tcaaaatctg ttgattggtc aaacctgatt tttttaactt atagatttca tagtttgtga
     1141 ttttcataaa taactccatt tctttttcta ctcatctatc tattcgtaaa agttcgtaaa
     1201 aaatcaaaaa acatccgact attttgtcag atgttggaat ctttaatttt cgtttttaag
     1261 aatttcttct aatttacgat agtcttggac actatcgatc tcataaatgc tattgccttc
     1321 taattcttca acatagacat ctagctcttt gatattatcc ttaaccatat tgtcccaata
     1381 gagatcaaca aattcaccac ttgcataagc cttgtcgata aagctgacaa tcttttctgc
     1441 agttggagca tcccagaagg atacaccact aaggatgcga cctgccttgc tatcaacaat
     1501 aatgtcttga accttgtagt catctccata gaccaagaac cattcgttgg tacaatcttc
     1561 acgataaaca ctaaaataag tcgaacgtgt caaatcattg cggaacatat ttttaaagag
     1621 ataattgtca gcatcaataa catagctgtt ggccaattct tcttttacaa gatagagaga
     1681 gtaaaagtta ttgtagtcag cgtatttatc attgaaaacg aggcgaacac cgtatttttc
     1741 ttttaagtaa tcgaattgtt ctttaagata accaacaatg atgatgatgt cattgattcc
     1801 tttttctttg agaaactcaa tttggtattc aatcaaaggt ttttgattaa cctgaaccaa
     1861 ggctttaggg gtattttcag tcataggacg caagcgagtt cccaatcccg ctgctaagat
     1921 aatggctttc acacgaatct cctttattct ttaataataa tataaactcc agcaatgacg
     1981 acaagtgacg tcataatggt cagcatatct agcggtgcac ccaaaaaaac aactgcaaac
     2041 aagaccgtcc atactacata gctcacgttc aagcctgtag ccttggctgg ttgcaagcga
     2101 ttgatagcga tataataagc caagtaggaa atcatatcaa aggctgcaaa aacaatcatg
     2161 agacctagca attgtccatt ggctactgca gtaaatgact gatgagagaa gagcacaatc
     2221 acaagatagg acaagaacga agtcacttga cggattaaga gggcttcgat ttcactcagt
     2281 tcactttcca tagcaaagga gctaagaaca ctctcacttc cccatgcaat agcacaaacc
     2341 aaagcacaga gaatcccaat gtagaaggaa ttaacctgtt caaccttata ggtctgggca
     2401 ataatccctc caataatcaa gacaatccca aatacagtat ttttcgaaat cttgtgcttc
     2461 aaaaagaaga aagccaatag aactgaaatc gcagggtaaa tagccgatac agatgaagct
     2521 aaagaacttc cgatatactt aactgcataa agattggcct gcataccgat agggcctgct
     2581 agcaaggctc cgatgataac actgacattg cgaatattta agaaaattga gaggcgaact
     2641 ttcccttctt ttaccaagag aaaagctagt aagataaaga tgctcaaaaa atcatgagct
     2701 gcagccacca caaagggtga caaatctgta aaaatcgaaa agatataagc actaaccgtt
     2761 agacccaagc cccagaaaat acctgagaga aggccaaaag gaactccgtt tttacttttc
     2821 atctgaacct ccataagaag ccaaaccttt aacagctctt tggtaacgat tcacaccata
     2881 gtcaccaaaa tcttcacctt gctcttcctt atagacagtc catagactcc aaatagtatc
     2941 ttgtaaaatt ttataaatag caatcttttc atgagaaacc ggtgtttggt cactctcata
     3001 gtgagataag aaagtttctt cctcttggga agtgaattca gactctaaaa agagggcagc
     3061 caaatcccac attggatcat tcattgatga atattcccag tcaatcaaat aaagtcgtcc
     3121 ttgaggtgat tcgataaagt tttcaggcac caaatcgata tgacaagatt ttctgtcaac
     3181 acctaagtca gccagtcttt tctctaagga gaagactgca tttctaacag attcatagtt
     3241 ggcataagga atttgttctt caatcaagga ttcgtatttt ttgatttctt caaaaggagc
     3301 aaattctcct cttaattcct tagcagacgt atgaatagtt tgtaatattg gagcaatttt
     3361 gtcgaacttg gtcttgattg acgttgaatc aagcgtaatc gcagattcga tatactcatt
     3421 tactttgata ccagcttcaa tatcaaaaag ataatttttt acatctaagc ctaaatcctt
     3481 tagtagttca agattgtact tttcatcttg tcgattgata agcttttctg tccctttacc
     3541 aaagaattta acaatgtatt gcttatttgt tgttttggcc aaatagtttt gattggtcat
     3601 tccacccagt tgttcaacac tgaggacttc ctcttcttga ctaagtaagg aagaaatttt
     3661 ttctttaatg attttctcca caattaacct ccagtactta tacttcccac ttaaacactg
     3721 ttttaaaggc tgtgtttaaa tcggttgcaa agacacgatg aatatcttta atttctctta
     3781 caggttcttc tagataaagg atatttttaa gacgattggc aaatttcttg acttccatca
     3841 tttggatagc atttttaaaa tcaatgcgac cagaacgaga tgacccaacc aaaatcaagc
     3901 ccttttctaa ggcatcgcga gtattgagat tgactttata ttcgctaact cccatcatga
     3961 gaatcgttcc ctgaggacga atgtagcgaa tcaagtcatt aatagctggt ccagtaccat
     4021 caccaccaca acattcaaaa gcatggtcaa aggccaactc ttcaggaata ttatccgtaa
     4081 tatagcattc tttggcaaat gagaagagtt ccaacttttc ccaatgacga ccaataacca
     4141 caatctctga ttctggcaaa gtatagttga taatattggc aaccacaaaa gctaaacttc
     4201 catctccaat aacggcgatc cgctcccgct tgctatgagc aagagtcaat agacgattca
     4261 tagcgtgcat gcccacactg acaaactctg taatggctgc aaccgtatct tcaatagcat
     4321 cataagccac cacacgatct ttagggagag aaacaaactc tctcataaag ccatcaaatc
     4381 cactagacaa gaaatgggtc cctgtcatgt agttttcata gaattcttca tcactctgca
     4441 taggagactg attgggaatc atgacaactt tttgaccaac ctcgtaggtt ccggtcgggt
     4501 cagaaatgac ggttccacat gactcgtgaa tcattgccat tggaagcttt ttattcaaaa
     4561 tcttgggatc acgttttccc tgatagtaac gctgatccgc atgacagaca gccatgtagt
     4621 tgggacggat aaggatatga ttctcttggt caatagcctc ttcctgatat ttgacattga
     4681 taaacttagg cttagttagt tgataaattt gattaatcat tttactagtc tttctcaatc
     4741 atactttttg caatcttcaa atctgttacg gttgtaatct tcagatttga gtattcacct
     4801 ttggccaagg ccacatcttt tcctttgatc acaaagattt tacatgcatc tgtcaagatt
     4861 tccttctctt catcagaaag agatccataa aggtccatga agtccttgca acggaatgtt
     4921 tgaggtgttt gtccttgata aaggtgagca cgatttggaa tatctgtaat aaattgacca
     4981 ttggtacttt caacgatagt atcaaccgct tctaccactg tgtccactgc gtcatgattt
     5041 tgggcaagtt ggatattgtc ctgaatcatg cgaagtgtaa taaatggacg aacagaatcg
     5101 tgggtaacaa cgatatcctc tggagtaagc ggacgataag catcaatggc ttcaatgatt
     5161 ttcttaatac ttgtattgcg gtcagcacca ccctttgtaa tgatgatacg ttccttataa
     5221 agaggaagat atttatctac aagatcttct gcatgagaaa cccagtctcc atgaacacca
     5281 actacaattt tttcaatact tggctccaag acaaattttt caattgtatg aatcaaaata
     5341 ggtcgattac ctagctctaa aaattgtttt ggcaagttac tgatccccat gcgtgtgcca
     5401 gttccaccgg caagaattcc tgcataaatc atctattttc tcctttgtct tactaaaaaa
     5461 acttattctc tctattatac cacaatctag tcctatcata aaagaaatac tggtcctccc
     5521 cttctagaat aggagaatta tgcagttcta ttattcaaag aaactagtcc atttctagta
     5581 atgactagga attctgtagc aattactaat aaatggctgc gaaattttag agtccttca
//
LOCUS       DAVLYR010000116         5565 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLYR010000116 DAVLYR010000000
VERSION     DAVLYR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.116"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(172..2169)
                     /gene="metG"
                     /locus_tag="VYO63_001606"
     CDS             complement(172..2169)
                     /gene="metG"
                     /locus_tag="VYO63_001606"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1672496.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2293..3552)
                     /locus_tag="VYO63_001607"
     CDS             complement(2293..3552)
                     /locus_tag="VYO63_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672497.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3554..4255)
                     /locus_tag="VYO63_001608"
     CDS             complement(3554..4255)
                     /locus_tag="VYO63_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672498.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4239..5438)
                     /locus_tag="VYO63_001609"
     CDS             complement(4239..5438)
                     /locus_tag="VYO63_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1672499.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
BASE COUNT         1592 a         1280 c         1013 g         1680 t
ORIGIN      
        1 tcagtttcat ctacaacctc aaagcagtgc tttgagctga cttcgtcagt ttcatctaca
       61 acctcaaagc agtgctttga tcaacccgcg gctagcttcc tagtttgctc tttgattttc
      121 attgagtata aataaaaaaa cagaactagc ctgaactagt cctgtctact tttacccaat
      181 cacacttcca tttggtacag ctggatcaac tgtgagaagg gttaatttgc catcatgttc
      241 agctgagaga atcataccct ggctgacata ttttttcatc attttacgtg gtttgaggtt
      301 agcaacgatt tgaactttct tgccgaccaa ttcttgttca tttggatagt attttgcaat
      361 tcctgaaaga atctgacgat cttctccatc accagcatcc aagcggaatt gaagcaactt
      421 atctgaacct tctactttag acacttcttt gacttctgcg acacggattt caaccttgtc
      481 aaagtcttca aacttgattt catccttgtt tagtttgagc tcaacttcgt ccggattcca
      541 ttctttttcg actgctggtt tattgccttc catttgttcc ttgatatagg cgatttcttc
      601 ttccatattt agacgtggaa agataggtgt tcctttggca actacagtca catctgctgg
      661 gaagtcagcc aaactcaagt tttcaagact agaaacttct tccaaaccaa gttgagtcaa
      721 aactgcacga ctagtttcca tcataaatgg ttcaatcaag tgagcaacta cacgaatgct
      781 ggctgccaag tggctcatga cacttgccaa ttggtcacga agagcttcat ccttgtccaa
      841 gacccatggt gcagtctcat cgatgtattt attggtacga gagatcagag tccagactgc
      901 ttcaagcgca cgtggatagt caactgcttc catgtgtgta tggaagtctg cgattgattg
      961 ttctgcaacc tcagcaagaa catgatcaaa ttcagtcaca ccttctacat aggcagggat
     1021 ttgtccatca aagtacttat taatcatgga aaccgtacgg ttaaggaggt tcccaaggtc
     1081 attagccaat tcatagttga tacggccgac atagtcttca ggagtaaagg ttccgtctga
     1141 accaactgga aggttacgca tgaggtagta acgaagtgga tctagtccat aacgctctac
     1201 caacatttca gggtaaacga cattcccttt tgacttagac atttttccgt ctttcatgac
     1261 aaaccaacca tgggcaatca aacgatcagg taatttaaca tccaacatca taagaaggat
     1321 tggccagtag atagagtgga agcgaaggat atcttttcct accatatgga agactgttcc
     1381 attccagaac ttgtcaaagt taccatgttc gtcttgagcg tagccaagag ctgtcgcata
     1441 gttaagaagg gcatcaatcc aaacgtagac aacgtgtttt ggatttgatg ggacaggcac
     1501 tccccatgta aaggttgtac gagataccgc caaatcttcc aaacctggct cgatgaagtt
     1561 gcgtagcatt tcattaaggc gaccatctgg cgtgataaat tcaggatgag ctttgaaaaa
     1621 ttcgaccaaa cggtcttggt atttgctaag gcgaaggaag tatgattctt cagaaaccca
     1681 ttcaacctca tgacctgatg gagcaatacc accagtcaca tttccagctt catcacggaa
     1741 aacttctgcc agctggcttt ctgtaaagaa ttcttcgtct gatactgaat accaaccaga
     1801 gtattcaccc aagtagatat catcttgagc aagtaagcgt tcaaagacct gtgcaacaac
     1861 tttttcatgg tagtcatcag ttgtacggat aaatttatcg tatgagatat ctagtaattg
     1921 ccagagttct ttaactccaa ccgccattcc atcaacatag gcttgaggtg taataccagc
     1981 ttcttccgct ttctgctgga ttttctgacc atgttcatca agacctgtca gataaaatac
     2041 atcgtagccc atcaggcgtt tgtaacgtgc taggacatca catgcgatag ttgtgtaggc
     2101 agaaccgata tgaagtttcc cagatggata gtaaatcggc gttgtaatat aaaaattttt
     2161 ttcagacata atttttcctt tccaggcaaa tgaaacctgt ttttctaaca cttcattata
     2221 tcacattttt aatgaatttc gatagggaaa tccatacaaa aacaagatag acgagtgtcc
     2281 atcttgttga tctcattcat aacgaagggc ttcaattgga tcaagtttcg atgccttgtt
     2341 ggctggcaag actccaaaaa tcataccaac actagccgaa actgcaagac taaatagggc
     2401 aactgggatt gatactccaa cttctatacc ttctattaaa ccttgcagta acaaacctgc
     2461 taaggcagtt aaaccacttg caattgtcaa gccaattaag ccacctaaca aggtcaaaat
     2521 catggattca atcaaaaact gaattaaaat attggcacgt gttgcgccca aagccttacg
     2581 aagaccaatc tcacgagtgc gctctgtcac cgaaaccagc atgatgttca tgacaccagt
     2641 tcctccaaca aagagagaaa tccctgcgat ggaactaata atcgtcgtca taaaactaaa
     2701 cgattgttga atttctgcaa atacaacgga ctcatctgcc acctggtatt ctccctgttg
     2761 taagcctgca agctctgtca tttttcgtgc cagttctgga cccagagttg gggttaaact
     2821 ggtatcattc actcgaaaga caatattagc tatttcatct acattaaaat tcgcagcaag
     2881 ggagatattg gtagtaatag gcaagccacc aaacccatat atttttgatc ttttagcctc
     2941 cggactagta taaaccccaa tgacccggta actaaatcca ttgacttcta caaccttgtt
     3001 aatagcctct tgaggagatt caaataaact aatggacaat tcctcatcta gcaaaatgac
     3061 acttgcaaac tctttgaaat cttgctctct cagactacga cctgcaataa tttcattctt
     3121 aacagcgtcc atgtaagttc tgtttccacc tgtcaaatta gcattctcaa cctttttatc
     3181 ttgataggtc aagatggcat tcgttgaatt ggttacatag taactatcca ctcccttcag
     3241 tttagctgcc tcttggaccc aggattcttg cggttttggc ggttcaacag gaacttcctc
     3301 ttcctttcca gaaaccgtaa aagctgattg tttctgagta aaagacccgt ctttactttt
     3361 tttaggagaa aaaaagacgc taatattttt ctgagattta gtcatatctt tattgacttg
     3421 acgagatagg gaatcaccca aagccataat cacaacaact gatgaaacac cgataataat
     3481 cccaatcata gtaagcaaag aacgcatctt gtgagccatg atagatgaaa aggcaaattt
     3541 cagattctgc atcttagttt tcctcctttc ctaactgagc actgtcagac gaaatgaccc
     3601 catcccgaat gacaatctga cgtttggcat aggcagcaat ctcaggctca tgcgttacca
     3661 tgataatggt ttttccttct ttattcaaat caaccaataa ttgcataatt tggttacctg
     3721 ttttggtatc caaggctcct gtcggttcat ccgctaggat aatagaagga ttgtttacca
     3781 aggcacgcgc aatggctaca cgttgctttt gaccaccaga taattctgaa ggtaaatggt
     3841 gactacgttc tgtcaattca accttgtcta aatattcctc agccaacttg cgacgttttg
     3901 aagacgaaac tcctgcgtaa atcaagggca attctacatt ttgcagagca ttgagcttcg
     3961 atagaagaaa gaactgctga aagacaaaac cgatttgttg gttacggacc ttagctagtt
     4021 gtttttcacc cagcccagcc acttcttgac cttcaagata atattctcca ctggttggtg
     4081 tatccaacat gccaatcgta ttcatcagag tggacttacc agacccagat ggtcccatga
     4141 tggctacaaa ttcaccctca ttcacttcta gattgatatt tttgagaacc tgcagttctt
     4201 ggtcaccatt acggtaactt ctgaagatat tttttagact aattagttgc ttcatcagcc
     4261 ttcacctctt ttccttcttc caaggaagat gttggattac tgatgacctt agcaccgttc
     4321 gttaaaccag aagtgatttc ttgattttct gcgtcagcat ttcccaatga aacctcaact
     4381 tttttagcct tttgttgttc atccacaatc cagacataat ttttactatc atccattact
     4441 agactgctaa caggaacaag aatagcctta gttttgcttt taacctcaat gttgacagaa
     4501 aaaccttgtt tcaaatcacc aacctcgcct gtcacatcaa tagtataagg gtatttagaa
     4561 cctgtattat tcccggctgc tggactagct gcttcaccat tgtttttagg atagtcagaa
     4621 atatagctta atttcccagt ccatttttta tcaggataca ctttagaagt aaagcttact
     4681 tcttgaccta cagaaaggtt ggctagattg tactcagaca attctccctt gacttgtaaa
     4741 ttttcattgc tgacaatatg aaccataact tggctcgccc ctgttggaga tttagaaaca
     4801 ttgctattga cttcgactac agttccctct agggtactga gaacagttgt tgcatccaat
     4861 tgactttgag ccttgcttaa ttgcgccgca gcatctgcac gcgcatcacg ggcatcaccc
     4921 aattgagcat caatagaagc aacagaattt ccagccactg gagttgggct ttgcaccgtt
     4981 gcatcttctc ctcctactgg cgctggtaac tgtggagccg gagctgaagc ggcttcattt
     5041 cgtgcttgat tgagttcatt gatatgacga tctgccctag ctactgctcg actagctgaa
     5101 tcataggccg cctgcgcttc tgaactactg tacttgacta aagcctgccc ttcgctgacc
     5161 ttatcgccca cagaaacaag gatttcatct aaatcaccct tactagcatc aaaataaaca
     5221 tattgttcat tttttgctgt tactgtccct gacaataaaa cagaggatgc cacgcttcct
     5281 tccttggcaa caacaagatg agtaggctca tcttttagag cagtctgaga aggttgtcta
     5341 aaaagtaaaa tccccccggc acccaataca actacactcg cagcaccgat tgctgcatac
     5401 agttgccact ttttagcttt accattcttt ttcttcataa tgaaactcct tttctttttt
     5461 acaatacttt gctattatac caaatttccc tccagcaaac aatgcagttc agatggaaat
     5521 aatggcagtt caggattaaa caatcgttcg gaattttgct tttct
//
LOCUS       DAVLYR010000117         5513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLYR010000117 DAVLYR010000000
VERSION     DAVLYR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.117"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            298..1248
                     /locus_tag="VYO63_001610"
     CDS             298..1248
                     /locus_tag="VYO63_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1672500.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTVLA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            1250..1993
                     /locus_tag="VYO63_001611"
     CDS             1250..1993
                     /locus_tag="VYO63_001611"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1672501.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            2004..3800
                     /gene="pepF"
                     /locus_tag="VYO63_001612"
     CDS             2004..3800
                     /gene="pepF"
                     /locus_tag="VYO63_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1672502.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            4047..4601
                     /locus_tag="VYO63_001613"
     CDS             4047..4601
                     /locus_tag="VYO63_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1672503.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            4712..5380
                     /locus_tag="VYO63_001614"
     CDS             4712..5380
                     /locus_tag="VYO63_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1672504.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         1541 a         1062 c         1321 g         1589 t
ORIGIN      
        1 ttctgacatc attattctct atggggtttc tttgggagaa actgataggt atatctggag
       61 tcagattgct gaacgatcaa tcagcggttc tgttcctgtc ataatttacc actatgtacc
      121 tcattttgat ccggggaatc ccattagagc aaaacgccta tatagaaatg tagaagataa
      181 attcatacag aatagtggta tagacttaga gttagagaaa aaattaagag acaaccttat
      241 tgtcgttatt ggtaagacta tttttgatct aatagagagg taattttaaa aaacaaaatg
      301 gaaacatggc aagagttaaa agttacagtg aagcgtgagg gagaggagtt ggtttccaat
      361 ctcttgattg agctgggagc gcaaggtgtt gcgattgaag acagtatgga ctatgtggga
      421 aatgttgacc gctttggcga gattttccca gaggtggagc agcaagaaga aatcgtagtg
      481 acagcctact accctgacac ggtggatgtg acagtggttg aggcggactt gcaggctcgc
      541 ttagcagaat tgacggattt tatggatttg ggagagttaa aaataggtac gactgtcttg
      601 gctgaggaag actgggcaga taactggaag aaatactatg agccagctcg tatcactcat
      661 gatttgacca tcgttccctc ttggacagac tatgaggcga ctgcggggga aatgattatc
      721 aagctggatc ctggcatggc ttttggtact ggaacccacc caactaccaa gatgagcctt
      781 tttgccttgg aacaggttct tcgtggtggc gaaacggtgc tagatgtggg gactggttca
      841 ggggttctct ctattgccag ctcgcttctg ggggctaaag aaattttcgc ctatgaccta
      901 gatgatgtag cagttcgtgt tgctcaggaa aatattgaac ttaaccctgg aatggaaaat
      961 atccacgttg ctgctggaga cttgcttaag ggtgttgaga ttgaggcaga tgtgattgta
     1021 gctaatatct tggcggatat tctcattcat ctgacagacg atgcctatcg tttggtcaag
     1081 gatgaaggct acctgatcat gagtggcatt atcaaggaca agtgggacat ggtgcgcgag
     1141 tcggctgagt cagctggatt tttccttgaa acccacatgg ttcaagggga atggaatgcc
     1201 tgtgtcttta agaaaaccaa ggatatttct ggtgtgattg gaggctagca tgcagcagta
     1261 ttttgtaaaa ggcagtgcta tctctcctgt cactatcgag gacaaggaaa ccagtaagca
     1321 tatgtttcag gttatgcgct tgaaagaaga ggatgaggtt accttagtct ttgatgatgg
     1381 catcaagcgc ttggcgcgcg tgctggatat ggaaaatcgt cagtttgagt tggtccaaga
     1441 attaaatgac aatgtggaac taccagttca agtgaccatc gcatctggat ttcccaaggg
     1501 agacaagctg gagttcatcg ctcaaaaagt aaccgaactg ggtgctagcc aaatctgggc
     1561 ctttcctgca gactggtcag ttgccaagtg ggatggcaag aaattgggta aaaaagttga
     1621 aaaactagaa aaaattgccc ttggagcagc cgagcaaagc aagcgtaata ttgtaccaag
     1681 tattaaactt ttcgagaaaa aagcagattt tctaactcag ctggaccagt ttgactctat
     1741 catagtagcc tatgaagaat cagctaaaga aggagaagcc gctgcgcttc tgcaagcagt
     1801 cactggtctt gaaacaggag ccaaactgct ctttatcttt ggtccagaag gcggtctctc
     1861 acctgcagaa atcgaaagtt ttgaagctaa gggagccgtt ttggtaggcc taggtcctcg
     1921 tattttgcga gcagaaacag caccgcttta cgccttatca gcccttagtg ttttagtaga
     1981 attagagaaa taagaggaag aaaatggaac aaaaacaccg ttcagaattt ccagagaagg
     2041 aactttggga tttaacagct ctataccaag accgtgagga tttcttgcgt gcaatcgaga
     2101 aagctcgcga agacatcaac cagtttagcc gtgattacaa gggcaatctt cacacttttg
     2161 aggatttcga gaaggccttt gcggaattgg aacagatcta cattcagatg agccatattg
     2221 gcaactatgc ttttatgcct cagacgacgg actatagcaa tgacgaattt gcaaatattg
     2281 ctcaagctgg gatggaattt gaaacagatg ccagcgtagc cttgaccttc tttgacgatg
     2341 ccttggtggt agcagacgag gaagtcttgg accgtttggg tgaattgcct cacttgacgg
     2401 cagctattcg tcaggccaaa atcaaaaaag cccactatct aggggctgat gtggagaagg
     2461 ccttgaccaa tctcggtgaa gttttctata gtccgcagga catttatact aagatgcgag
     2521 ctggggattt tgaaatggct gactttgaag cccatggcaa gacctataaa aacagctttg
     2581 tcacctatga gaatttctac caaaatcacg aggatgctga ggttcgtgag aaatccttcc
     2641 gttccttctc agagggactt cgtaagcacc aaaatacggc tgcagcagcc tatctggctc
     2701 aggtcaagtc tgaaaaactc ttggctgata tgaagggata cgactctgtc tttgattatc
     2761 ttctagctga acaagaagtg gaccgtgtca tgtttgaccg ccagattgac ctcatcatga
     2821 aggactttgc accagtcgct cagagatacc tcaagcatgt tgccaaggta aatggtcttg
     2881 aaaagatgac ctttgcagac tggaaattgg acttggacag cgccctcaat cctgaagtga
     2941 ctattgacga tgcctatgat ttggtcatga agtcggtaga acctttgggg caagaatatt
     3001 gtcaggaagt tgctcgttac caagaagagc gctgggtgga ctttgctgct aacagtggca
     3061 aggattccgg tggttatgcg gcggacccat atcgcgtaca cccttatgta ctcatgagct
     3121 ggacaggccg tttgagcgat gtctatacct tgattcatga aatcgggcat tctggtcaat
     3181 tcatcttttc agacaatcac caaagctatt tcaacgccca catgtcgacc tattatgttg
     3241 aagcaccgtc gaccttcaat gaattgctac tcagtgatta cttagagaac cagtctaatg
     3301 acccacgtca aaaacgcttc gctctggctc atcgcttgac agacacctac ttccataatt
     3361 tcatcaccca cctcttggaa gccgccttcc agcgtaaggt gtatacattg attgaagaag
     3421 gagaaacctt cggagcaagc aaactcaaca gcattatgaa ggaagttttg acagattttt
     3481 ggggcgatgc cattgaaatc gacgacgatg cagctttgac ttggatgcgc caagctcact
     3541 actatatggg cttgtatagt tacacttact ctgctggtct tgtcatctcg acagctggtt
     3601 accttcatct taaaaactca gaaactggag ctgaagactg gctcaatctc ctcaaatcag
     3661 gtggtagcaa gacaccactt gagtcagcca tgattatcgg tgctgatatt tcaacagaca
     3721 aaccactccg tgataccatc caattcttgt ctgacacagt tgaccagatt atcgcctaca
     3781 gtgctgagtt gggggagtaa ggggaataga aaaattttct agaatgatgt ttatgggctt
     3841 gttaagcgtc attctatttg tcttagttgt agctattttc aaaaatgtcg ttgaaggtcg
     3901 tgatgttaaa tttgaactta ctgtagaact ggtattgata gttatttacg tcattttata
     3961 tcagattgta tttaattgag aattcaatat tgagtcattt aagactgcct ttttgaacag
     4021 ttgctagaaa tgtagaggta ttgaccatgt atcaagattt actaagaaaa atagcagaag
     4081 aaaaaccaaa ttataaccag gaagaaatcc agtggttgtt tgatcatttg ggaaatcctt
     4141 ctccagaaat tcgtgatgac ctttcaaatc aaggcttgca ctatctttca aaagaaaagg
     4201 atacaacagg tttctcaagt cagtatggtt gggttcacgc ttttgcacat ggagccgatt
     4261 tactgacaga ggtggtttgt catccagact ttcctaaaaa cagagttcat gaagtatttg
     4321 atatacttgg ccaactattt aaaagaatgt cgattcgctt tacagatgat gaggattggc
     4381 gtttagcaag agtgatctat gaacctattt tacaagggaa gttggagcaa gaacaagtag
     4441 cttcttggat aaaaactgtt gattttccga tagaagaaag ggaggatttt tacaaatttt
     4501 ccaactttag atcctgtctg gtggaagtct atgtccaact tgaccagaga aatagtttac
     4561 aagatgagtt gaaagaagct atccagtctt ttcaatacta gagattagct aatagttcta
     4621 tgattttcaa aaaatttcca atcaattttc ttgaaaccct ttcctacttt tgatagacta
     4681 gtatctagtt tttgaaaaaa ggagaaagaa aatgaaaaaa tttgttgctg agttaatcgg
     4741 tacgttcatg cttgtgttcg ttgggacagg agctgttgtt tttggaaatg gtcttgatgg
     4801 ccttggtcac cttggaatcg cctttgcctt tggtttggca atcgtggtgg cagcctactc
     4861 aatcggaact gtttcaggtg ctcacttgaa cccggctgtt tcgattgcta tgtttgtaaa
     4921 caaacgtttg tcatcttcag aacttgtaaa ctacatcctt ggtcaggttg ttggagcttt
     4981 catcgcttct ggcgctgtct tcttcctctt ggctaactca ggtatgtcaa ctgctagtct
     5041 tggtgaaaat gccttggcaa acggtgtcac tgtctttggt ggtttcttgt ttgaagtcat
     5101 cgcaactttc ttgtttgtat tggttatcat gactgtgact tcagaaagca agggcaatgg
     5161 cgcgattgct ggtttggtaa tcggtttgtc attgatggcg atgattcttg tcggattgaa
     5221 gattactgga ctttcagtaa acccagctcg tagcttggca ccagctgtct tggtaggcgg
     5281 cgcagccctt caacaagttt ggattttcat ccttgcacca atcgctggtg gagttcttgc
     5341 agcccttgtt gcaaaaaatt tccttggaac agaagaataa ttgaaactca aaaagccttg
     5401 ctcctcatct tgaggaacag ggctttttcg tatgatactc ttcgaaaatc tcttcaaacc
     5461 acgtcagctt caccttgccg taggtatggt tactgacttc gtcagttcta tcc
//
LOCUS       DAVLYR010000118         5472 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLYR010000118 DAVLYR010000000
VERSION     DAVLYR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.118"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            463..1140
                     /locus_tag="VYO63_001615"
     CDS             463..1140
                     /locus_tag="VYO63_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1672505.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLCKEVSPVFVQKWQKEAVKLEFALGQIPDKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            1127..1924
                     /locus_tag="VYO63_001616"
     CDS             1127..1924
                     /locus_tag="VYO63_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1672506.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLEIEETTYLQETGPERGIGRIGNIQPQTFGELAQHVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            1941..3044
                     /locus_tag="VYO63_001617"
     CDS             1941..3044
                     /locus_tag="VYO63_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1672507.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            3345..4364
                     /gene="galE"
                     /locus_tag="VYO63_001618"
     CDS             3345..4364
                     /gene="galE"
                     /locus_tag="VYO63_001618"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1672508.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4366..5301
                     /locus_tag="VYO63_001619"
     CDS             4366..5301
                     /locus_tag="VYO63_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1672509.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTNWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSYHRS"
BASE COUNT         1588 a         1022 c         1283 g         1579 t
ORIGIN      
        1 ctagcgacct gtaattaaac aagtaaaaga acaatactta gggagacctg tgacaatgat
       61 atcttcctct ggaagtagtt ctaattcaaa caatgtttgt tccatgactg tatcctcctg
      121 ttatgataaa ttccagaaag catagagcaa attcgtggtt cgtctgacta aatttcttat
      181 ctgaacacca ccttcaaata caaattttag tcaaactttt attttttcta actttctaat
      241 tatattctac catgtgtttt tatatgtcaa gaattttctg aataaactgt ttatttttta
      301 ttttttgaca tttttgcaaa ttgtttaaaa tctttcaatt ggattcactt attactttag
      361 gacattgaaa cttctttcgc cagaggtgct tatattactg ggaagttgaa gattgaaatg
      421 tggtataata agaggtaata gagttttgga aagtgagaga agatgatttc aaagagatta
      481 gaattggtag cttcctttgt gtcacagggg gctattttac tagatgtggg aagtgaccat
      541 gcttatctgc ctatcgagtt ggttgagaga ggccaaatca aaagcgctat tgcaggtgag
      601 gtggtggaag gtccctatca gtctgcggtt aaaaatgttg aggctcacgg cctaaaggag
      661 aaaatccaag tccgtttagc caatggcttg gcagcttttg aagagactga ccaagtgtct
      721 gtcattacca ttgctggcat gggtggtcgt ttgattgcta ggattttaga agaaggtttg
      781 gggaagttag ctaatgtaga gcgtttgatc ctccagccca ataatcgtga agacgacttg
      841 cgtatctggc tacaggatca tggattccag attgtagcag aaagcatctt agaagaagct
      901 ggaaagtttt atgagatttt ggtggtggaa gcaggacaaa tgaagctatc agccagtgat
      961 gttcgctttg gaccgttctt gtgcaaagag gtcagtccag tctttgtcca aaaatggcaa
     1021 aaagaagctg ttaagctaga gtttgccctc ggacaaatcc cagacaaaaa tctggaagaa
     1081 cgtcaagttc tagtagataa gattcaagcc atcaaggagg tgctccatgt tagcaagtga
     1141 agtgatccaa gcttatgaag ccttttgccc tcaggaattt tctatggagg gagacagccg
     1201 tggtctgcaa attggcactt tagacaaggg tatccaaagg gtcatggttg ccctcgatat
     1261 tcgtgaagag acagtggcag aggccattga aaagggtgtg gacttgatta tcgtcaagca
     1321 cgcgcctatc tttcgtccta tcaaggactt gcttgccagc cgtccgcaaa atcagattta
     1381 catcgacctg attaagcatg acatcgcagt ttatgtcagc cataccaata ttgatatcgt
     1441 tgaaaatggg ctcaatgact ggttttgtca gatgctagaa atcgaggaga cgacttatct
     1501 gcaggaaaca ggtccagaac gtggaattgg acgtattggg aatattcagc ctcagacctt
     1561 tggagaattg gcccaacatg tcaagcaagt ctttgaccta gatagccttc gaatggtgca
     1621 ttatcaagag aatgatttgc agaagcctat ttcaagagta gcaatttgtg gtggaagcgg
     1681 gcagtctttc tataaggatg ctttggcaaa gggggcagat gtctatatca ctggtgatat
     1741 ctactaccat actgctcagg atatgctgtc tgatggcttg ttagcattgg atccaggtca
     1801 ctatatcgaa gtgctttttg tggaaaaaat cgcagcactc cttactcaat ggaaagagaa
     1861 aaaaggctgg gaactagaga ttttacctag tcaagcatcg accaatcctt tccgtcatat
     1921 ctagttagaa agtgaagaca atgaaaaaag ttgccattat tggagcagga attgtgggag
     1981 caacagctgc ctactacctc tcgagagaaa gtgacctaga ggtgaccgtt tttgaccatg
     2041 agcaaggtca agccaccaag gccgcagcag gaattatcag tccttggttt tccaaacgcc
     2101 gtaataaagc ctggtacaag atggcgcgct tgggggctga tttttatgtg gatttattag
     2161 ctgatttaga gaaatcagga caagaaatcg acttttacca gcgttcggga gtctttctct
     2221 tgaaaaagga tgaatccaat ttggaagaac tttatcaact ggccctccag cgcagagaag
     2281 aatctccctt gatagggcaa ttagccattc tgaaccaagc ctcagctaat gaattattcc
     2341 ctggtttgca gggatttgac cgcctgctct atgcttctgg tggagcgaga gtagatggcc
     2401 aactcttggt aactcgtctg ctagaagcca gtcatgtcaa gctggtcaaa gaaaaagtga
     2461 ctctgacacc gttagcatca ggctaccaga ttggtgaaga ggagtttgag caggttattt
     2521 tggcgacggg agcttggttg ggggacatgt tagaaccctt gggttatgaa gtagatgttc
     2581 gtcctcaaaa aggacaacta cgagattatc agctggtcca agatatggaa gcttaccctg
     2641 ttgtcatgcc agaaggggag tgggatttga ttccttttgc aggtgggaaa ttgtccttag
     2701 gcgctaccca cgaaaatgac atgggatttg atttgacggt agatgaaacc ttgctccaac
     2761 aaatggagga ggccaccttg actcactatc tgattttggc tgaagctact tcaaaatctg
     2821 agcgtgttgg aatccgtgcc tacaccagtg atttctctcc tttctttggg caggtgcctg
     2881 acttaactgg tgtctatgca gccagtggat taggttcatc aggcctcaca actggtccta
     2941 ttattggtta ccatttagct caactgatcc aagacaagga gttgaccttg gaccctgaaa
     3001 actacccaat tgaaaactat gtcaaacgag taaaaagcga ataatactca atgaaaatca
     3061 aagaacaaac taggtaacta gccgcgggtt actcaaagca ctgctttgag gttgtggata
     3121 gaactgacga agtcagctca aaacactgtt ttgaggttgt agatagaact gacgaagtca
     3181 gtaaccatac ccatggcaag gcgacgttga cgtggtttga attggatttt cgaagagtat
     3241 aagcatttta ctgaaatttt atcaggtagt ttaggatggc aaatgacatt ccctgtcaaa
     3301 aatggtaaaa taagaaaaaa taatctgaga atcgaggaaa aaagatgcaa gaaaagattt
     3361 tggtaactgg tggtgccggt tttattggaa cccacactgt tattgaattg atccaagcag
     3421 gccatcaagt tgttgtggtg gataatcttg tcaatagcaa tcgtaagagt ttagaagttg
     3481 ttgaaagaat tacaggagtt gagattcctt tctatgaggc agatattcgt gacacagcta
     3541 ccctcagaga tattttcaag caggaagaac cgactggtgt cattcacttt gctggtttga
     3601 aggctgttgg cgaatcaaca cgtatccctc ttgcctacta tgacaacaat atcgctggaa
     3661 ctgtcagcct tttgaaagcc atggaagaaa acaactgtaa aaacatcatc ttcagttctt
     3721 ctgcgacagt ttacggggat ccgcacacag tgcccatctt ggaagatttc ccactttcag
     3781 tgaccaaccc atacggtcgt accaagctca tgctagagga aattttgacc gatatttaca
     3841 aagcagactc agaatggaat gttgtcttgc ttcgttactt taacccaatc ggagcccatg
     3901 agagtggtga tttgggagaa aatccaaacg gtattccaaa caatctcttg ccatatgtga
     3961 ctcaagtagc cgttggaaaa ttagagcaag tgcaagtgtt tggagacgat tacgatacgg
     4021 aagatggaac aggtgttcgt gactatatcc acgttgtcga tttggctaag ggtcacgttg
     4081 cagctttgaa aaaaatccaa aaaggttcag gactaaacgt ttataacctt ggaactggta
     4141 aaggttactc agttcttgaa attatccaaa acatggaaaa agcggtggga cgtcctattc
     4201 cttaccgtat cgtagaacgt cgcccaggtg atatcgctgc ctgctactca gacccagcaa
     4261 aagctaaagc agaactcggt tgggaagcag aactcgacat cacccaaatg tgtgaagacg
     4321 catggcgttg gcaaagcaag catccaaatg gatttgaaga ctaagatgat gatttcaatc
     4381 atcgtccctt gtttaaacga agaggaagta cttcctcttt tttatcaggc tctggaagct
     4441 ttacttccag atttggaaac agaaatcgag tatgtctttg tcgatgatgg atcaagtgat
     4501 gggaccttgg aactcttaaa ggcctatcgg gagcaaaatc cggcagtcca ttatatttct
     4561 ttctctcgaa attttggcaa agaagcagcc ctttatgcag gcttgcaata tgcgacagga
     4621 gatttggtgg tggtgatgga tgcagacctc caagatcctc ctagtatgtt gtttgagatg
     4681 aaaaatgtac tagacaaaaa tgtagacttg gactgcgttg ggacacggag aactagtcgg
     4741 gagggagaac ccttctttcg cagtttctgt gctgttctct tttatcgcct catgcaaaaa
     4801 atcagcccag tagctctgcc gtcgggtgtc cgtgattttc gtatgatgag aaggtctgta
     4861 gtcgatgcca ttttaagctt gaccgagtcc aatcgttttt caaaggggct cttcgcctgg
     4921 gtgggtttca aaacctacta tttggactat ccaaatgtcg aaaggcaggc tggcaagaca
     4981 aattggagtt ttaggcaact ctttttctac tccattgaag ggattattaa tttttcagat
     5041 tttcccttga ttatagcctt tgtagctggt ctcctatctt gttttctttc tctgctgatg
     5101 accttttttg ttgtggttcg gaccctcatt ttgggcaatc cgacatctgg ttggacctct
     5161 ctgatggctg ttattctctt tcttggaggt attcaactct tgaccattgg gattctcggc
     5221 aagtatatca gtaagattta tttagaaacc aaaaaaagac cgctttatct tatcaaagaa
     5281 aaaagttatc atagatcatg aaaaagtagg agttatctag cgcagtcact tttttgtaat
     5341 tataattctt ttggtctgga acttgagtga tttttaaaaa aattagggat ttgacatgat
     5401 ataaatgaac tatgtggaat aaatttactg caaagacgat aacttaataa ataaaaaatt
     5461 caggagggtt at
//
LOCUS       DAVLYR010000119         5333 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLYR010000119 DAVLYR010000000
VERSION     DAVLYR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.119"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            10..537
                     /locus_tag="VYO63_001620"
     CDS             10..537
                     /locus_tag="VYO63_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1672510.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRVRLFLEKKEK"
     gene            614..970
                     /gene="glnR"
                     /locus_tag="VYO63_001621"
     CDS             614..970
                     /gene="glnR"
                     /locus_tag="VYO63_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1672511.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            1007..2353
                     /gene="glnA"
                     /locus_tag="VYO63_001622"
     CDS             1007..2353
                     /gene="glnA"
                     /locus_tag="VYO63_001622"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1672512.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2449..2568)
                     /locus_tag="VYO63_001623"
     CDS             complement(2449..2568)
                     /locus_tag="VYO63_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1672513.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            3113..4432
                     /locus_tag="VYO63_001624"
     CDS             3113..4432
                     /locus_tag="VYO63_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1672514.1"
                     /translation="MPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDT
                     EKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAIS
                     NYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRII
                     EAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLL
                     EKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYI
                     QRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPKTVHSYQKERLLRDGDLMWNS
                     TGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEE
                     KASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            4489..5286
                     /gene="psrA"
                     /locus_tag="VYO63_001625"
     CDS             4489..5286
                     /gene="psrA"
                     /locus_tag="VYO63_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1672515.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT         1743 a          859 c         1047 g         1684 t
ORIGIN      
        1 gttgagatta tgagttattt taaaaaatat aaattcgata aatcccagtt caaacttggt
       61 atgcgaacct ttaaaacagg tattgctgtt tttctagttc tcttgatttt tggctttttt
      121 ggctggaaag gtcttcaaat tggtgctttg acagccgttt ttagcctgag ggagagtttt
      181 gatgagagtg ttcattttgg gacttcgcgt attctaggaa atagtatcgg tggactctat
      241 gccttggtct tcttcttatt aaataccttt ttccacgaag ccttttgggt gaccttggta
      301 gttgttccaa tctgcaccat gttaaccatt atgacaaatg tagccatgaa taacaaagca
      361 ggggttattg gtggtgtagc agctatgtta atcattaccc tatcaattcc aagtggtgag
      421 acaatttcgt acgtgtttgt gcgtgtatca gaaacgttta tgggagtttt tgtcgcaatt
      481 atcgtaaatt acgatattga tcgtgttcgt ctctttttag agaaaaaaga aaaataatgt
      541 tacattttat aacattatca attgacgttt gtcttttttt agactataat agacagaaag
      601 aaggaaattg taaatgaagg aaaaagaatt tcgccgaaat atggctgttt ttcctatcgg
      661 cagtgttatg aagttgaccg atctatcggc gcgtcagatt cgttattatg aagatcaaga
      721 gttgatcaag cccgatcgaa acgaaggaaa tcgtcgcatg tattccttga atgacatgga
      781 tcgtctgctt gaaatcaaag attatatctc tgaaggttat aatatcgctg ccattaagaa
      841 aaaatatgct gaacgtgaag cgaaatccaa gaaagcggtt agtcagactg aagtacgtcg
      901 tgcacttcac aatgaactcc tccaacaggg gcgttttgct tcagtacagt caccttttgg
      961 tcgcggttag gcaaccgcaa gtagtcatag attaaggaga aaacttatgc caatcacagc
     1021 tgcagatatt cgtcgtgaag tcaaggaaaa aaatgttacc tttattcgtc ttatgttctc
     1081 agatattttg ggaaccatga aaaacgtcga aattcctgct acagatgaac agttagataa
     1141 ggttttgtcg aacaaggtta tgtttgatgg atcttctatt gaaggttttg tacgtatcaa
     1201 tgagtcggat atgtacttgt acccggactt ggatacatgg acagtcttcc cttggggaga
     1261 tgaaaatgga agtgttgcag gtctgatctg tgatgtctat acaacagaag gtgaaccatt
     1321 tgcgggtgac cctcgtggta atttgaaacg agctcttcgt cacatggaag aagttggatt
     1381 caaatccttc aaccttggtc cagagccaga attcttccta tttaagttgg atgaaaatgg
     1441 ggacccaaca cttgaagtga atgacaaggg tggctacttt gatttggcac ctactgacct
     1501 tgcggacaac acacgtcgtg agattgtgaa tgtcttgacc aaaatgggat ttgaagtaga
     1561 agcgagtcac cacgaggttg cggttggaca gcatgagatt gactttaagt acgatgaagt
     1621 tctccgtgct tgtgataaga ttcaaatctt taaacttgtt gttaaaacca ttgctcgcaa
     1681 acacggactt tacgcaacat ttatggcgaa gccaaaattt ggtattgctg gctcaggtat
     1741 gcactgtaat atgtccttgt ttgatgcaga aggaaataac gccttctttg atccaaatga
     1801 tccaaaagga atgcagttgt cagaaacagc ttaccatttc ctaggcggtt tgatcaagca
     1861 tgcttacaac tatactgcca tcatgaaccc aacagttaac tcatacaaac gtttggttcc
     1921 aggttatgaa gcgcctgttt acattgcttg ggctggtcgt aaccgttcgc cacttgtgcg
     1981 cgtacctgct tcacgtggta tgggaactcg tcttgagttg cgttcagtgg atccaatggc
     2041 gaacccttac attgctatgg ctgttctttt ggaagttggt ttgtatggta ttgaaaataa
     2101 aatcgaagca ccagctccta tcgaagaaaa tatctacatc atgacagcag aagagcgcaa
     2161 ggaagctggt attacagacc ttccatcaac tcttcacaac gctttgaaag ctttgacaga
     2221 agatgaagtg gttaaagctg ctctcggaga tcacatctac actagcttcc ttgaagccaa
     2281 acgaatcgaa tgggcaagtt atgcaacctt cgtttcacaa tgggaaattg ataattattt
     2341 agacctttac taatactaat atagaagaaa gattgcctgt gggtaaacag tcacacagca
     2401 gtctaagcca tcaaatacct ataaccctta aaatagcgat ttcggtcgct atttattttt
     2461 cgaaaaatcc tcttgactct tttgcgaatt agatttatca tcagacgtgt agaagaaaat
     2521 taaggagtca ctatggaaaa attcaatttc aaaaacaata tcgggcaaga aaacaagtta
     2581 cttcaaatcg aaatctacaa gtttactaac ttttgcaagc ttcaaaatta cacatctgta
     2641 aatatttttt cgaaagatat atttgaagcc atagtaaatt gagactagaa tggtacacta
     2701 catctgctaa aaatttctag aaactaattt aactttccta atcgatttgt ttacatctta
     2761 tttcaatcta ctatattatc tcaataaatg atgatggcga ctgttttgtt tgtgcttgtc
     2821 gatttttgaa tcctgatcga gcggaagaat atttctgccg aggttgccaa ggggaaaatc
     2881 ctgaagatat tgaattttat gatgaacaat tacaggcaga aaaggtggag gttttgaata
     2941 ttcgattgga agtaaaaaat tgaattatct acaatctcat caacaataaa cctatttcaa
     3001 ccccattatc tataggcgta tttttacgcc tttttatata cttgaagcac ataagaaaca
     3061 ttatgggaag tttgctgatg gaagtactca agaaattgat gttccttatg atattcctga
     3121 tacttgggag tgggtgaggt tttctacatt ggttgaaatt gtcagaggtg gctctccacg
     3181 accaatcaaa gattatctta cttctgaagt agatggaata aattggataa aaataggtga
     3241 tactgaaaag ggtgaaaagt atataaataa tgttaaagaa aaaatcaaaa aatcagggct
     3301 taacaaaact agatttgtaa aaaaaggtac atttttgtta actaattcta tgagttttgg
     3361 tagaccttat attttgaatg ttgatggtgc aatacacgat ggatggttgg ctatttcgaa
     3421 ctatgaaaac tcattaaata aagattacct attctatatt ctttcatcaa atgtagttta
     3481 ttctcaattt ctatctctaa ttagtggagc tgttgtgaaa aacttgaata gtgataaagt
     3541 tgcttctatt cttatccctc tccccccact atccgaacaa caacgcataa tagaagcaat
     3601 cgaatcagct ttagaaaaag tagatgaata tgctgaaagt tataatagac tagaacagct
     3661 agataaagaa tttccagata aactaaaaaa atctattctt caatatgcta tgcaaggaaa
     3721 attagttgaa caagacccaa atgatgaatc agtcgaagtt ttacttgaaa aaatacgagc
     3781 agaaaaacaa aaactctttg aagaaggcaa gattaaaaag aaagatttgg acatttctat
     3841 tgtttcccaa ggagatgata actcttatta tgaggaagta ccttgtgaaa taccagaaag
     3901 ttgggaatgg gtgaggttaa acgatataac ctcctacatt caaagaggga agtctccaaa
     3961 atattcaaat attcctatat accctgtgat tgctcagaaa tgtaatcaat ggtctggctt
     4021 ttctattgac ttagcaagat ttattgatcc caaaacagtt catagttacc agaaagaaag
     4081 attactaaga gatggtgatt taatgtggaa ttcaacaggt ctaggtacgt taggacgatt
     4141 agctatttac catgaaaata aaaatcctta tggctgggct gttgcagata gccatgttac
     4201 agttataaga gtcttatcgg gggttattaa ttgccatttc atctataatt ttctttcctc
     4261 tcctattgtt cagtcagtta ttgaagaaaa agcgtcgggt agtacaaaac aaaaagaatt
     4321 attaacgaaa actataaaag aatatttaat ccccctccca ccccttcctg aacaatccag
     4381 aatcgttgat aaaatcgaac aatttttcgc ccatattgac gcactaattt agtataatag
     4441 ttttacgtgc ggtggaattt ctatcaaaca taagatagga gttttacgtt gaaagatttt
     4501 gaattacatt tgaagaaagc tggtttagct gaaaatactg tgcgttcgta cctttatggt
     4561 gttcgatttt tcttggagaa ttacgaactc aaaatggaag atttatttga atacaaagga
     4621 tatttattag acaattttaa acctaaaact gtcaatttgc gactacaagg agtaaataaa
     4681 tatctggctt ttattggtca tgatgatttg aaattaaaat ttgtcaaagt acagcaaaaa
     4741 ccgtttttag aagatgtcat tagccatgct gattaccttt ttcttaaacg tagtttgaaa
     4801 aaagatggta ttttaaaatg gcattttgtc gtttggtttt taggtgcaac tggggctcgt
     4861 gtaagtgaac ttattaaact aaaagtagag catgtagaaa tcggttattt tgacatttac
     4921 tcaaaaggtg gtaaaattcg tagactgtac attcctaaaa aattaagaaa tagttgtctt
     4981 agttggttag aatcagaaaa tcgccgaagt ggttatttat ttttgaataa atttaacgaa
     5041 ccaattacag caagaggagt tgctcaacag ttaaaaaatt atgctgataa atacaaaatg
     5101 aatcctaaag taatttaccc tcattctttt aggcatttat ttgctaagaa ttttttagcg
     5161 aagtataatg atattgcctt gcttgcagat ttgatgggac acgaaagtat agaaactact
     5221 cgaatttatc taaggaaaac agctactgaa caacaaaata ttgtagataa aattgttaat
     5281 tggtaaaaaa taacaggtgg tcaaactgac tacctgctat ttttgtgatt atg
//
LOCUS       DAVLYR010000120         5283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLYR010000120 DAVLYR010000000
VERSION     DAVLYR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.120"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            123..1445
                     /locus_tag="VYO63_001626"
     CDS             123..1445
                     /locus_tag="VYO63_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1672516.1"
                     /translation="MNNILTFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRIFIQRGITIKMPNGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            1447..3333
                     /locus_tag="VYO63_001627"
     CDS             1447..3333
                     /locus_tag="VYO63_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV1672517.1"
                     /translation="MVIRVFDQQKNTYSSFALEELRYYMNQVFNTDIELVEEKEADIF
                     VGLVNEEDGKDHIFISLNKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFIP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYKDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            3501..3845
                     /locus_tag="VYO63_001628"
     CDS             3501..3845
                     /locus_tag="VYO63_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1672518.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            3855..5267
                     /gene="lacG"
                     /locus_tag="VYO63_001629"
     CDS             3855..5267
                     /gene="lacG"
                     /locus_tag="VYO63_001629"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1672519.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVQYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
BASE COUNT         1666 a          749 c         1112 g         1756 t
ORIGIN      
        1 caattaaaat tattattcta tacaaagaaa aaaatattgt aaaaacagta aaaaatatat
       61 tgacaacgct ttctgttagt ggtatattta atttataaaa aataaaaaaa ggagttccgg
      121 tgatgaacaa tattttaacg tttttagaaa cgaaagtcgc tccgtttggt gaaaaagttg
      181 gcaatcaacg gcatttgaaa gctattcgtg aaggatttat gatggcaatg cctttgattt
      241 tagtcggctc tttatttctt attctaatca gttggcctca agaggctttt acaaattggc
      301 tgaatagtgt tggattgcta agtatcttga caactatgaa tcagtcaaca gtagcgatta
      361 tctccttggt cgcttgtttc ggtattgcct acaggttgtc ggaaggatat ggtacagatg
      421 gtccgtctgc agggatcata gccttatcca gttttgtatt gatggcccct cgtttttcaa
      481 gtatggttta tgataaaaat ggggagcagg tcaagcagtt atttggaggt gcaataccat
      541 tttctagcct gaatgcgtct tctttgttta tggcgattac cattggattg gttacagcgg
      601 agatttatcg tatttttatc caacgcggaa ttacgataaa aatgccaaat ggtgtcccag
      661 atgtggtcag taaatcattt tcagctcttt tacctggttt tactactttt gttttgtggg
      721 ctttggtctt aaaaggtctg gaagcagcag gagttgcagg aggtctcaac ggacttttag
      781 gtgcaattgt tggaacgccg cttaagttaa ttgcaggaac acttccaggt atgattttat
      841 gtgttattgt aaactcattc ttttggttct gtggagttaa tgggggacaa gttttaaatg
      901 cttttgtaga cccagtttgg ttacaattta ctacagaaaa ccaagaagct gtggctgcag
      961 gacaaaccct acaacacatt attacattac cgtttaaaga tttatttgta tttattggtg
     1021 gcggtggagc gactattggt cttgcgattt gtctcttcct atttagtaag agtcgtgcta
     1081 ataaaacatt aggtaagcta gctattatac cgtctatctt taatatcaat acagctattc
     1141 tatttacgtt tccaacggtt ttaaatccta ttatgctgat tccgtttatt gctactccta
     1201 caatcaatgc cttgattacc tatgtatcaa tggctgtagg cttagtaccc tatacaacag
     1261 gtgtaatcct tccgtggaca atgccaccta tcataggagg cttccttgcg acaggggcta
     1321 gttggcgagg agctctatta caagttgttt tgattttggt ttctgtagca atttattatc
     1381 cattcttcaa aattgcagat aaacgcaatc ttgaaaaaga aaaagctact gttggaggga
     1441 aataagatgg ttatcagagt atttgatcaa cagaaaaata cttattctag ctttgcctta
     1501 gaggaattaa gatactatat gaatcaggta tttaataccg atatagaact tgtcgaggag
     1561 aaggaagcgg atatttttgt aggattagtc aatgaagagg atggaaaaga ccatattttc
     1621 atctcattaa acaaaggtaa ggggagaatt gagtccaata caattgtagg tttacttatt
     1681 ggaatttacc gaatgtttca tgaatttggt gttgtgtata ctagaccagg gcgcggacat
     1741 gactttattc cagagttaag atttgaagat tttttagata aagagctatc tatagatgaa
     1801 acagcaagtt actatcatag gggagtatgt atagagggag cagattcatt tgaaaatata
     1861 ctagatttca ttgattggct acctaagatt gggatgaaca gttttttcat tcagtttgaa
     1921 aatccctatt cttttttgaa acgttggtat gaacatgagt ttaatccata cctaaataaa
     1981 gaaaaattct caaatgaatt agtacaagaa ttgagtgata ggttggataa agaattgcaa
     2041 aaaagaggtc ttattcatca tcgtgttggt catggatgga caggtgaggt tttaggttac
     2101 tcttcaaaat ttggctggga atcaggtctt agtatttcag aggagaagaa accctatgtc
     2161 gctgaaataa acgggaaacg agaattgttt aatacggctc cgattttaac cagcctggat
     2221 ttttcaaatc cagatgtagc tgataagatg gtagaaatta ttaaggatta tgccaagaaa
     2281 agacctgatg ttaactactt acatgtatgg ttgtcggatg ctcgtaataa tatttgtgaa
     2341 tgcgaaaact gtagacaaga attggtttcg gatcagtata ttcgtattct caatcaattg
     2401 gatagggctt taacgagtga gggattagat acaaagattt gtttcctact ttaccatgag
     2461 ttgttatggg cgcctcagaa agaaaaatta gataatcctg aacgctttac catgatgttt
     2521 gcaccgatta caagaacatt tgaaatgagt tatgcagatg tagattttga caattccata
     2581 cctacgccta aaccttatct gcgtaataaa attatacttc cgaattctct tgaagaaaat
     2641 ttatcttatc tttttgagtg gcaaaaaaca tttaaaggag atagtttcgt atatgactat
     2701 cctttagggc gtgctcatta tggcgattta ggctatatga aaattagtca aactatttac
     2761 aaagatgtat cttatctttc aaacctacat ttgaacgggt acatttcgtg tcaagaatta
     2821 cgtgccggat tccctcataa ttttcctaat tatgttatgg ggcaaatgct ctggaagaag
     2881 acaagaagtt atgaagaatt gattgaagaa tacttttctg ctttgtatgg gtcaaattgg
     2941 cagtctgttg ttgaatattt agaaaaatta tctagttatt cctcatgtga ttattttaat
     3001 gcaattggca gccgtcaaaa tgatgtttta gcgaatcatt attatatagc ttacaatcta
     3061 gctgataatt ttttaccaat tattgaggaa aatatttcta agttattaaa tagtcaaaag
     3121 gatgaatgga aacagctcag ttatcatcgt gaatatgttg ttaagatggc gaaggcttta
     3181 tatcttcaag caactggaaa aacaaggcaa gctcaagatg aatggagaaa tgttttgaat
     3241 tatatccgtg gtcacgaatt gctattccaa tctaatttgg atgtttatcg tgtaattgaa
     3301 gtagcaaaaa attacgctgg tttccactta taaattataa gtatagaaaa tgaactaagg
     3361 tattcagaga agattgatcc taaatattat gaaatttaag gatttttaag atatttaggg
     3421 tcaactttct atttgtatcg tagcgaagtc attttaataa tgatgtgtaa aagatgtatc
     3481 aagattgagg aggaagaaag atgaaatcaa aagaagaaat aaatatgctt ggttttacaa
     3541 ttgtcgctta cgcaggagat gcaaggtcag atttgatgga tgctttggcg tttgcgagag
     3601 atggatattt tgaacaggca agagaattgg ttgagtctgc aaacgactca atagtgtctg
     3661 cccatcgaga acagactaat ttattagcgg aggaggcatc tggagataat tttgaagtga
     3721 gttttatcat gattcatggt caagatactt tgatgacaac gatgctattg tatgatcagg
     3781 taaagttttt tattgatgaa tatgaacgaa ttcgaaagat tgaagaacat attggtttgc
     3841 aatgaggatt agtcatggaa aatttacagg ttaaagcctt accgaaggag tttttattag
     3901 gagctgctac cgctgcttat caagtagagg gtgcaactag ggtagatggc aaaggaataa
     3961 atatgtggga tgtttatttg caagaaaata gtccgttctt accagatcca gctagtgatt
     4021 tttattatcg ttacgaagaa gatatagcct tggcggcaga acatggtttg caggctttgc
     4081 gtttatctat ttcttgggtt cgcatatttc ctgatataga tggggagccg aatatattgg
     4141 ctgttcagta ttaccataga gtctttcagt cctgcttaaa acataatgtg attccgtttg
     4201 tttctttaca tcattttgat tcgcctcaga aaatgttaga aacaggggat tggttgaata
     4261 gagagaatat tgatcgtttc atacgatatg ctcgcttttg tttccaagaa tttacagaag
     4321 tcaagcattg gtttacaatc aatgaactga tgtctcttgc tgcaggtcaa tatataggag
     4381 gtcagtttcc tccgaatcat cattttcaat tatctgaagc aattcaagcg aatcataata
     4441 tgttgttggc gcatgctctt gcagtcctcg aatttcatca attagggatt gagggaaagg
     4501 taggttgtat tcatgcttta aagccaggct atcctattga tgggcaaaaa gaaaatattt
     4561 tggcagctaa acggtatgat gtttataata ataaatttct attagatgga acttttttgg
     4621 gctactacag tgatgacacg ctttttcact taaatcaaat attggaagct aataattcta
     4681 gctttattat tgaagatggt gatttagaaa ttatgaaaag agctgcacct cttaatacga
     4741 tgtttgggat gaattattat cgttcagaat ttattcgtga atacaaaggt gaaaatagac
     4801 aagaatttaa ttcaacagga ataaaaggac agtcctcttt taaattaaat gctctaggtg
     4861 aatttgtaaa aaagcctggt attccgacaa cagattggga ttggaatatt tatcctcaag
     4921 gcttatttga tatgttactt cgtatcaaag aagaatatcc tcaacatcct gtcatttatt
     4981 taactgaaaa tggtacagcc cttaaagagg ttaagccaga gggcgagaat gatattattg
     5041 atgacagtaa gagaatccgt tatattgagc aacatttaca caaagtttta gaggctcgag
     5101 ataggggagt caatattcaa ggatatttta tatggtcttt gcaagatcaa ttttcttggg
     5161 cgaatggcta caataagcga tatggtcttt tctttgttga ttatgagaca cagaagcgat
     5221 atattaagaa aagtgctctt tgggtaaaag ggctaaaacg gaattaaggt tagcgatttg
     5281 aat
//
LOCUS       DAVLYR010000121         5121 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLYR010000121 DAVLYR010000000
VERSION     DAVLYR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.121"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            18..1013
                     /locus_tag="VYO63_001630"
     CDS             18..1013
                     /locus_tag="VYO63_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1672520.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQ
                     KFSPEMMVMVKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKSAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGIKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            1039..1683
                     /locus_tag="VYO63_001631"
     CDS             1039..1683
                     /locus_tag="VYO63_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1672521.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1725..3713)
                     /locus_tag="VYO63_001632"
     CDS             complement(1725..3713)
                     /locus_tag="VYO63_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672522.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3715..4473)
                     /locus_tag="VYO63_001633"
     CDS             complement(3715..4473)
                     /locus_tag="VYO63_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672523.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4901..5092
                     /locus_tag="VYO63_001634"
     CDS             4901..5092
                     /locus_tag="VYO63_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672524.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1596 a         1014 c         1018 g         1493 t
ORIGIN      
        1 acaagaaagg cacagagatg caagacaatt atactacaaa agccaaacat ttgacaatcg
       61 atagccgtcg cttaatcgaa agatggaaaa aagaagggaa atcaaataga gaaattgcct
      121 ctctgcttgg aaaagttcct caaactatcc acactgaaat caagcgtggg acagtccgac
      181 aatgtcttgg aaaagggcgc ttcaaagagg tttattctgc cgactatgct caacagtctt
      241 atgaaaacaa tcgcaagcgc tcggtcagga aatcaagcgt gaccaaggaa ctaaaggaaa
      301 agattctcca ctatcataac caaaaatttt cgcctgaaat gatggtgatg gttaaagggg
      361 ttaacgtggg aatttcaacc atttactatt ggattcatca tggaaaattg gggttaagca
      421 aacaggatct gctttaccct agaaaaggaa aagcgcttaa gaaacaggct agcaccaact
      481 ttaaatctgc tggtcaatcc atcgaacagc ggtctgaagc tatcaatctt cgcttggaga
      541 atgggcatta tgagattgat acggttctac ttacgagagc gaaaaactac tgtttgcttg
      601 tcttgacgga tcgaaagagt agacatcaga tcatccgatt gattccaaat aaaagtgctg
      661 atgtggtcaa tcaggctcta aaactcatct taaaacaaca caagattctt tccatcacgg
      721 cagataatgg aacggaattc aatcgcttgt ttgatgtatt ttctgaggag cacatctatt
      781 atgcgcaccc ctatgcctct tgggaaaggg gaactaatga gaatcacaac aggctcattc
      841 gtagatggtt acctaaggga atcaagaaaa tgactcccaa agaagtcgca ttcatcgaaa
      901 agtggattaa caactatcct aaaaaatgct tggactacaa gtcacccaga gaagacttct
      961 ggatggctaa cttgaacttg aaatttagcg tcagaaacaa atcacgaaat tgattcaaat
     1021 tttgcaggtc gtttaaatat cctgcgtgcg ggtgttcttg atgctaacga tggaattatt
     1081 tccattgctg gtgtggttat cggagttgcc agtgccacga gcaatatctg gattatcttt
     1141 ttatcaggtt ttgcggctat cttagctggt gccttttcaa tggctggtgg agaatatgta
     1201 tccgtttcaa ctccaaaaga taccgaggaa gctgccgttt cgcgagaaaa actcttgcta
     1261 gaccaagata gggaactagc caaaaaatcc ctctatgctt cttatatcca aaatggagaa
     1321 ttcaaaactt ctgcccaact cttgactaat aagatctttc ttaaaaatcc actcaaggct
     1381 ctggtagagg aaaaatatgg gattgagtat gaagaattta ccaatccttg gcacgctgcc
     1441 atttctagct tcgttgcctt tttccttaga agtttgcctc caatgctgtc agtgaccatt
     1501 tttccaagtg attaccgcat ccctgctacc gtccttattg tcggtgtggc ccttcttctc
     1561 actggttaca caagtgctag acttggaaaa gccccaacca aaacagctat gattcggaac
     1621 cttgctattg gtctcttgac catgggagtt accttcctgc tcgaacaact tttcagcatt
     1681 tagaatacaa gaaatacctc gattttgaag tcgaggtatc ttttttacat ttgcacaatc
     1741 ttgcgataac ttcttgaagt aatcataaaa atcagcacat aggcgatgag gaagatagcg
     1801 cagatagaca aggtcacaat caacatcata gtcgtatcca gtacaccaat cacttttaaa
     1861 atcaggctaa gcatatggta ggcaaaggct agatgtagga aggcaaagag caaaggaagg
     1921 aagaaaacag ttaaaacctg tttgttgatg gtttgcttga tttgcttttg gtccaaaccg
     1981 actttctgca agataataaa gcgttcacgg tcttcgtagc cttcagaaat ttgtttgtag
     2041 tagatgacca gaacagttcc gaccataaag ataatggata ggaaaatacc gataaagaag
     2101 acaccaccaa agagggcact catctgagca ctagcatctg ctagagtgct accataaaca
     2161 tagctacctt ctgtgtctaa ttgagcatta aattgattga ggtagttttc atactcctca
     2221 gcgaccttga gttgttcttc ttcgctggca tctacattca taccaccgta aaactgatta
     2281 tagatagccg aatctgggaa ttgatccaaa aaggcttgta aatcaggaac aacaaggtaa
     2341 ttgtaatcag tagtcaagat attaaactta tttggaacat ggttgacaat gaaatcttta
     2401 ttaaattctt ctttgacaga aaattgatga tcatttagag ttagagcttt ctgtcctttc
     2461 agtccgtcat ttttggcaaa gagaccgacc tcatttcctg atagagacag tttttgacca
     2521 gtcatatttt cataatcttt ttggtcaaat accatgaaaa ctgtttttgg ttggacacgg
     2581 ttttgtcctt tttcaaaaat agttaacttg gttccttctt gatttgcaat accaaagtta
     2641 ctgtaacgaa gcacttcttt ctctttaatc ttataacctt tgtcacttgc aaactggctc
     2701 aagagtttgt ccaaatcttc tttttcaaca ttttgccctg aaaccccaaa atcatgagga
     2761 tttagaactt ttttaaagga ttctgcggaa ttgaaaatgc ttgtcgctgc tgacatggtt
     2821 accaaaacca ttgttgacaa aatagcgata gttgctagtc caactgcatt tttcttcata
     2881 cggaaaatca agttagaaac agatatgaga ttattaggtt ggtaatagta tttcttattt
     2941 ttctttaaga tttgaaggaa aacggtaatc cctgcattga acaaaagata agtaccaaag
     3001 ataaccagta aaacagctat gaagaaggtt gttaaggctg taagaggatc ttttaccgta
     3061 agggcaagat aatatccaat ccctaaactt atggaaccaa gaatagtttg gagaggaagg
     3121 aagcgacctt ttttctctcc gctagctttc tcacgtgaga gctggagggc attcatacgg
     3181 gcgattcgaa gggcattcag gaacatgagg cctaggaaaa tcaaaccgaa gacaagaagt
     3241 actgtaatga caactttcat ctggaaggta gcgaccagtt ccaccttcaa tttcatcagt
     3301 ttgagtagga aagcgaaaat caacttgtca aacaaggctc caataccgat acccgctcca
     3361 acagttagaa tcccaaatac cactaactcc ttaaaggtca tactgataag atgacgcttc
     3421 tccaagccca acatgccata aattcctagt tccttggaac ggttcttcat gacaaaacta
     3481 ttggcataga gaacgataat ggctgacgca agggtgacga caaacatacc aaatccaagt
     3541 gtagcctgaa tggttgttcc tccacggatt tccgcaatct taggattgaa ggttagagag
     3601 taaaagagat aggtgacagt gactgccaag agaacagcca gcgcaaaagg atagtagagt
     3661 ttgcggtttt taatcaagtt cgataccgct aacttattgg ttaatcgaaa catactaatt
     3721 cacctcgctt gccatgacag tcaaggtatc agagatttct tggaacatct gacgttctgt
     3781 cttctctcca cggtagattt gattgtaaag aatgccgtct ttgataaaga gtacacgttt
     3841 agctctgcta gctgctgctg ttgagtgggt taccatgaga atggtttggc cgcgctcatt
     3901 gatttcatca aagacatcaa gtaaggctgc agatgacttg gagtcaagag ctcctgtcgg
     3961 ttcgtccgca aggagaattt caggttctgt gatgatggcg cgggctactg ctacacgctg
     4021 tttctgacca ccagaaatct cgtaagggta cttctcttgt aattggttta ttcccagatt
     4081 ctcagctgtc accaccaatt tcttcatcat ctctgtaata ggtcttcttg acaagacaag
     4141 cggaagcaag atattgtcct ttacagatag agtatctagc aagttaaagt cttggaagac
     4201 aaagcccaac ttttcacgac ggaagctaga ggcctgtgaa tttttaatgg ttgcggtgtc
     4261 agttccattc aagtaaacct gaccacgact tggtttatcc agcatagcta gaatattgag
     4321 aagagttgat ttaccagaac cagactcacc catgatggca acgtagtcac ccttttcgac
     4381 ggtaaagtga atatccttga gggcttctac ttggttgccc tgaaaacgtg ttttataaat
     4441 tttttgaacg tgttttacat ctaaaagtgt catgagaatc tcctttctta atatcccatc
     4501 aaatgaaatg tagcttgcat catagtgcat cccagctgaa cgcgctcaat ttctttgtga
     4561 cttggttttc tagttttctt atgtaagatt tgtttcatga tgttactcct ttgttcttta
     4621 tgagtctagt ttacatcaaa aaaagaccgc ttgccataac ctaaccttac aaaagagact
     4681 ttaatcttac atttttgtaa gattgggtga aatataagca aaattactta gaaaatgatt
     4741 tgatttccta tttttaaagt tgtaaaatat aagtgtgaaa aggcttactt tgaaaaggag
     4801 gaagcgggaa ctaacatttt agtaaggtta cctagataac ataccattca atttaacact
     4861 tgcaagtcgt tcaacctata aggaaagaag gtgcgcttta atgaaacaca aagaacatat
     4921 actcatcgga cttctctatc tgctctctcc attcatcggt cagctcttag ttgaacacac
     4981 acactttatc ggtacagaat ttaccggcac tgcttatgtt atttgctggt tatcagttgt
     5041 tatcagcatc caccattttt ccaaaaatgt attgtctcaa cagcaaaaat agattaaaaa
     5101 actgcgttta aattactgct t
//
LOCUS       DAVLYR010000122         4849 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLYR010000122 DAVLYR010000000
VERSION     DAVLYR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.122"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            60..656
                     /locus_tag="VYO63_001635"
                     /pseudo
     CDS             60..656
                     /locus_tag="VYO63_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            813..1181
                     /locus_tag="VYO63_001636"
     CDS             813..1181
                     /locus_tag="VYO63_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1672525.1"
                     /translation="MAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
     gene            1194..1580
                     /locus_tag="VYO63_001637"
     CDS             1194..1580
                     /locus_tag="VYO63_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672526.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYAD"
     gene            1587..1676
                     /locus_tag="VYO63_001638"
                     /pseudo
     CDS             1587..1676
                     /locus_tag="VYO63_001638"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1662..2285
                     /locus_tag="VYO63_001639"
     CDS             1662..2285
                     /locus_tag="VYO63_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672527.1"
                     /translation="MDEPLSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAI
                     SDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNIIPTNLEKRSDGAYIVFSDG
                     YALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASK
                     IQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            2275..3920
                     /locus_tag="VYO63_001640"
                     /pseudo
     CDS             2275..3920
                     /locus_tag="VYO63_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4023..4715)
                     /locus_tag="VYO63_001641"
     CDS             complement(4023..4715)
                     /locus_tag="VYO63_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1672528.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1520 a         1038 c          837 g         1454 t
ORIGIN      
        1 tttaaaaacg cttacattgt tttaaaataa acttaaataa cacaacggag atatccatca
       61 tgaaaaagaa atggatgtat tatgctgctt gttcttctaa tgaatctgcc gatgacagtt
      121 catctgataa aggagacggt ggttcgctag tcgtttattc accaaactca gaaggcttaa
      181 ttggagcaac tattcctgcc tttgaagaaa aatatggtat caaaatagaa ctgattcaag
      241 ctggtactgg agaacttttc aaaaaactag agtcagaaaa agaagttcct gtagctgatg
      301 ttatctttgg tggttcttat acacaatatg ctacccacgg agaactcttt gaaaactata
      361 tttcaaaaga aaatgataat gttatcaaag aatatcaaaa cacaactggc ttctctactc
      421 cttatacact agatggtagt gttttaatcg tccaccctga tttaactaaa ggcatgaaca
      481 tcgaaggata tagcgacctt ctcaaacctg aactaaaagg aaaaatcgca actgctgacc
      541 cagcaaactc ttctagcgcc tttgctcaat taacaaatat gctacaagct caaggtggtt
      601 acaaagatga taaggcttgg tcttatgtaa aagatctttt cacacttatt gatggtatag
      661 taaaatgaaa taagaacagg acaaattaat caggacagtc aaatcgattt ctaacaatgt
      721 tttagaagta gaggtgtact attctagttt caatacacta taaaatcggt tcaagttcat
      781 ctggtgtcta taaagcagtc gttgatggag aaatggctgt tggtctctct tatgaggatc
      841 cagcagttaa actcttaaat gacggagcta acattaaagt agtctatcca aaagaaggaa
      901 ccgtcttcct acctgctagt gctgctatcg ttaaaaaagc taaaaatatg gaaaatgcca
      961 agaaatttat cgattttatt atctctcaag aagtacaaga tacacttggt acaaccacta
     1021 ctaaccgtcc tgttcgtaaa aatgctaaaa caagcgaaaa catgaaacca attgacaaaa
     1081 tcaaaacact cactgaagat tatgattatg tcatcaagaa taaatcagat atcgttaaga
     1141 aatacaacga agtctttaca gatatccaat ctaaacagta aaagaggttc actatgagtg
     1201 agatcgaaat tattaacgcc aaaaaaatct accacgatgt ccctgttatt gagaatttga
     1261 acattacaat tccaaaagga agtctcttta cccttcttgg agcttcagga tgtgggaaaa
     1321 cgacccttct tcgtatgatt gcaggtttca acagtatcaa agatggagaa ttttacttcg
     1381 atgatacaaa aatcaataat atggaaccca gcaaacgcaa tatcgggctg gttttccaaa
     1441 actacgctat tttcccacat ttgactgtcc gagacaacgt tgcttttggt cttatgcaaa
     1501 agaaggttcc aaaagaagaa ttgattcaac agaccaacaa gtatcttgaa ctcatgcaaa
     1561 ttgctcaata tgcggattga aagcccgata aactcagtgg tggacaacaa caacgtgtca
     1621 ccttggcatg cgccttagcg gttaatccaa gtgttctcct catggacgag ccacttagta
     1681 atctggaggc caaacttcgc ttggatatgc gtcaagccat ccgagaaatc caacacgaag
     1741 tgggaattac aactgtttat gtaacccacg accaagaaga agccatggct atttcagacc
     1801 aaattgctgt tatgaaagat ggggtgatcc aacaaatcgg ccgaccaaaa gaactctatc
     1861 ataaaccagc taatgagttt gtggcaacct ttatcggacg cacaaatatt atccctacta
     1921 atcttgaaaa acggagcgac ggcgcttata tcgtcttttc agatggctat gcccttcgaa
     1981 tgccagctct tgatcaggtt gaggagcaag ctattcatgt aagcattcgt cccgaagagt
     2041 ttatcaaaga tgaatctgga gatattgaag gaactattag cgatagcgtc tatcttggac
     2101 taaatacgga gtatttcatt gagacaggtt ttgcctcaaa aattcaagtt agtgaagaat
     2161 caacttttga agaagaacta caaaaaggca atcgtattcg tctacgaatc aatacgcaaa
     2221 aattaaacat cttttctgca gatggttccc aaaacctgat aaaaggagtc aaccatggaa
     2281 tgtaaaaaac taaatatttg gacaacctcc tctttcttca tctttcttac ctatcttgtc
     2341 tttctcgttt atcctatcgt taccgtgctc aagcaagcac ttatacatga aggacaattc
     2401 tcactagcta attttgtcac tttctttagt aaagtctact actctgagac actagtcaac
     2461 agtttcaagg tttccattac cgctactgtc acttccttag ttgtaggaac cctattagct
     2521 tatctcttct ctatgtatga cttcaagggg aagaaatttc tacaaatatt gattatcatt
     2581 gcttccatgt cagctccttt cgtaggagcc tactcctggg ttctcttgct aggacgaaat
     2641 ggggtcatca ctaaattttt gacaaatgcc cttcatcttc cagctatcga tatttatgga
     2701 ttcaaaggaa ttgtacttgt ctttacactg caactattcc cactggtatt tctatatgtt
     2761 gctggaacaa tgaacagtat tgacaattct ctacttgaag cagctgaaag catggggtcc
     2821 ttcggattta aacctatcgt aacggttgtt ttacctctcc tagttccaac cttactagca
     2881 gctccttgct tgtatttatg agagcattct cagactttgg aacgcctatg ttgattggcg
     2941 aaggatatcg gaccttccct gtcctgattt atacccaatt tattagcgag gttggaggaa
     3001 attctgcttt tgcaattatg gcgattatca ttgtcttggc aattttcctt atccaaaaac
     3061 acattgcaaa ccgctacagt ttcagcatga atctgctcca tccaattgag cctaaaaaaa
     3121 ctacaaaagg aaaaatagct gccatttatg caacagtcta cggaattatc tttatctctg
     3181 ttttacctca aatctactta atttatacct ctttcctaaa aacatcaggt atggtatctg
     3241 ttaaaggtta ttctccaaac agttacaagg tagctttcaa tcgtatggga tctgctattt
     3301 tcaataccat tcgtatccct ttgattgcct tagttctagt tgttctattt gcgacattta
     3361 tctcctacct agccgttaga aaacggaatt tgtttacaaa cttaattgac agcctcagta
     3421 tggtacctta tattgtacca ggaaccgttc tagggattgc cttcatttct tccttcaata
     3481 ctggtctatt tggaagtgga tttcttatga ttacagggac tgctttcatc ttgattatgt
     3541 ctctatctgt cagaagatta ccttatacta ttcgctcatc tgttgctagc ttacaacaaa
     3601 tagcaccaag tattgaagaa gctgctgaaa gcttaggaag tagtcgtctc aatacctttg
     3661 ctaagattac aactccaatg atgctatctg gtatcatttc tggagccatc ttatcttagg
     3721 tcataatgat ttcaaaactc tctacttcta tcctcctcta caatgtcaaa acaagaacaa
     3781 tgactgtagc tatttataca gaggttctca gaggaaatta cggtgtagcc gcagccttgt
     3841 caactatcct gactgttcta acagtaggtt ccttgctctt gtttatgaaa atctctaaaa
     3901 gcaatagcat tacactttag ttttccacat caaaaacagc cgaaaggagt accctcggct
     3961 gttttttctt atcttgatat tcttatcaaa tccctagctc atggcaagca agtctagact
     4021 gattaaatag agccttcttc catcttctca cgtcctagct ctcggtaact acggtcaccg
     4081 acaacttcaa ccctaaactg accgtcctca tattcaagaa tcgtcacgct accattatct
     4141 agaccatgcg gatgcatgcc attaatcaga taaacaatgg ttccaatagt cattccatgg
     4201 ctgacaacaa gggcgttacc tccaccttga tcttccattt cttttgcaat catttcaaag
     4261 ccttccttga ttcggccact gagtttttcc cagccttcag cccaaccagc tgtatcgacc
     4321 tctaccaagc cctcagccag ttcagcataa gacaattggt gaacgtggtc cacattaaag
     4381 atacgaggaa taatgcccat gaaaagatcg ccatcatagg ctccatcaaa actaccgaaa
     4441 caccattctc tgatacgctt gtccatgcga taagggattt tcccctgcaa gccaagttct
     4501 tcaaggataa ttcccatggt ctgaatggta cgaccagaat cactcgaata agcgcgctca
     4561 aactgtagat cagattctcg caaaccaatt cctaactctt gaatccctcg ttcaccttca
     4621 gcagttaagg gagtatcgct ccaaccttgc gcgcgaccaa tcgtgttaaa catggtcttg
     4681 ccatgacgta ccaaatacaa tcgtactttt accattttcc gtcctccgtt tgcttctatt
     4741 atatcatgaa tgataaaata aaagccaccc atacaggcga cttttgaagg agattattat
     4801 gaaaaagttt aggagttcat taaataaaga tattagatga aaatcaaat
//
LOCUS       DAVLYR010000123         4786 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLYR010000123 DAVLYR010000000
VERSION     DAVLYR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.123"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(75..1646)
                     /gene="ffh"
                     /locus_tag="VYO63_001642"
     CDS             complement(75..1646)
                     /gene="ffh"
                     /locus_tag="VYO63_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1672529.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(1658..1990)
                     /locus_tag="VYO63_001643"
     CDS             complement(1658..1990)
                     /locus_tag="VYO63_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1672530.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(2080..2457)
                     /locus_tag="VYO63_001644"
     CDS             complement(2080..2457)
                     /locus_tag="VYO63_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1672531.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDGQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            2529..3833
                     /locus_tag="VYO63_001645"
     CDS             2529..3833
                     /locus_tag="VYO63_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1672532.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            3846..4652
                     /gene="yidA"
                     /locus_tag="VYO63_001646"
     CDS             3846..4652
                     /gene="yidA"
                     /locus_tag="VYO63_001646"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1672533.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMADKEIVKCMFIDEPE
                     ILDATIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
BASE COUNT         1385 a         1151 c          881 g         1369 t
ORIGIN      
        1 tgatactgta cgttttatca tagaaatttt tactttattt tctcatcaaa tgagatttgc
       61 atcaatctct tgtcttactt gcgtttcttc ttcgctttct tcattttgtt agccatacgt
      121 ttcatggact gtttcatggc aaattcacca attttacctt tcaaaccgcc accaaacatc
      181 tggctcatat ctggcattcc tgctcctccg agagctgata agtcaggcat accgccttgt
      241 cccatcattc cttcaagggc agacatatcc attcctccca tatttggcat atttttagga
      301 aggttatttg gattaatccc catttgcttc atcattttat tcatatcccc agacataaca
      361 ccctgcatga gctgtttagc ctggttaaag tccttgatga atttattgac ttcgacgaat
      421 gtatttccag aaccagcagc aatacgacgg cgacggcttg gatttaacaa atctgggttt
      481 tcacgctctt caggtgtcat cgaagacaca atggcacgtt tacgagcaat ctggcgttca
      541 tccaccttca tgttttgaag ggctggattg ttggccatac ctggaatcat cttgagcaag
      601 tcttccatcg gccccatatt ttgcacctga tctaattgat cgatgaaatc attaaaatca
      661 aaggtgtttt cgcgcatctt ctcagccatt tcaagggctt tttgttcatc gtattcctga
      721 gaagctttct caatcaaagt gagcatatcc cccataccaa ggatacggct agacatgcgg
      781 tctgggtgga aggtttcaat gtccgtaatc ttttcacctg taccagtgaa cttgattggt
      841 tttccagtaa tgtgacgaac agacagagca gcaccaccac gagtatcgcc atcaatcttg
      901 gtaaggatga ccccagtcac ttccaactga gcattaaact cacgcgcaac attggctgct
      961 tcctgaccaa tcatagcatc aacgacaagc aagatttcat ttggttgagc caatgctttc
     1021 acatcacgaa gctcattcat gaggagctca tcaatctgca aacgacccgc agtatcaatc
     1081 aagacatagt cgttatgatt agtttgggct tgctccaaac cttgacgtac aatctcaaca
     1141 gctggtactt ctgttccaag tgcaaagaca ggcacatcaa tctgttgtcc caaggtctta
     1201 agctggtcaa tggcagctgg acgataaata tccgccgcaa tcatcaaagg acgagcattt
     1261 tcttctttct tgagtttgtt ggccaattta ccagcaaagg ttgttttacc agccccttgt
     1321 aaaccaacca tcatgatgat ggttggaatc ttaggtgact tgataatttc tgccgtatca
     1381 gaacctaaaa cggctgtcag ttcctcatca acgattttaa taatctgttg cgcaggatta
     1441 agtgtatcaa tgacctcatg cccgactgca cgctcacgaa ctttcttgat aaagtccttt
     1501 acaacaggca aggcaacgtc ggcctcgagc aaggccaagc gaatttcttt ggttgcctct
     1561 tggacatcag attcagagat ttttcctttt ttacgtagat ttttaaagac gttctgcaaa
     1621 cgttctgtta aactttcaaa tgccattttt cttcctctta ttctctatta tcaatgcttg
     1681 ttaaaatttc tatctgctcc tgcagaaaat catccttggg atagcgctcc aagatttggt
     1741 caaaaatctg actacggaca atgtagtccg agtacatgtg caatttcatc tcataatctt
     1801 ccagaatctt ttctgttcgc ttgatattgt catagacagc ctgacgacta acaccaaact
     1861 cctcagctat ctcagcaaga ctgtaatcat cagcgtagta aagctcgata taattcattt
     1921 gcttatctgt caaaagcgcc gcataaaatt caaagagcgc attcatacga ttggtttttt
     1981 cgatttccat aacttttatt ataccaaaaa atagcctaat ctaccacact agggagccaa
     2041 tccttgaaga tagaaagtag atttgagaaa aacgagatcc tagccccaag taatttccaa
     2101 ttgatagctg gcaaagggat gcccctcttg attttgtagt tgataagcta gctcaatctt
     2161 ttgcccatct acttgataac ggctcgtttg aatgataaat tcctgcatgc ccataggggt
     2221 aggaatatag gccaaactat cactatcctt taaaaagcgc ataatggtct tgggattgga
     2281 aaatcggctc atcataagtt cttgaccatg aaatttaatc actacttttt ccttttcctc
     2341 attatagaaa agcaggtagc tataatctcc tttttcatgc acttccacat cataaagctg
     2401 gtcaatcact tccaactgct catcaaactg aattgtattt cgcatccgaa tcttcacatc
     2461 aggccctctt tcttgtctct tgtcctacta ttttaccaaa aagagcagga ttttgctata
     2521 atggtcatat gaacgaaaaa gtattccgtg accctgttca caactacatc catgtcaata
     2581 atcaaatcat ctatgacttg attaatacaa aagaatttca gcgtttgcgc cggatcaaac
     2641 aactgggaac ttccagttat accttccacg gtggagaaca cagtcgcttc tctcactgtc
     2701 taggagtcta tgaaattgca cgacgcatca cagagatttt cgaagaaaaa tatcctgagg
     2761 aatggaatcc tgccgagtct ctcttgacca tgaccgctgc tctcctacac gaccttgggc
     2821 atggtgccta ctcccatact tttgaaaatc tctttgatac agaccatgaa gccattactc
     2881 aggagattat tcaaaatcct gagacagaga ttcaccaagt cctgctacaa gtagcgcccg
     2941 attttccaga gaaagttgcc agtgtcatag accataccta tcctaacaag caggtcgtgc
     3001 agctcatttc tagtcagatt gacgcagatc gcatggacta tctcttgcgc gactcctatt
     3061 ttacaggagc atcctatggg gaatttgacc tgactcgaat cctccgagtc attcgtccta
     3121 tcgaaaatgg tatcgccttt cagcgcaatg gcatgcacgc catcgaagac tacgtcctca
     3181 gtcgctacca gatgtacatg caggtttatt tccaccccgc aacacgcgcc atggaagttc
     3241 tcctacagaa tcttctcaaa cgcgccaagg aactctatcc tgaggacaag gatttctttg
     3301 cccgaacttc tccacacctc ctgcctttct tcgaaaaaaa tgtgaccttg actgactatc
     3361 tggctctgga tgatggtgtg atgaatacct acttccaact ctggatgacc agtcctgaca
     3421 agattcttgc agatttatcg catcgctttg tcaaccgcaa ggtctttaaa tccattacct
     3481 tttcacaaga agaccaagac caactcgcta gcatgagaaa attggttgag gacatcggct
     3541 ttgatcccga ctactatact gccattcata agaactttga cctcccttat gatatttacc
     3601 gtcccgaatc tgaaaatcca aggacacaga ttgagatttt acaaaaaaat ggagaactgg
     3661 cagaactctc tagcctgtcc cctatcgtcc aatcccttgc tggaagtcgc cacggagata
     3721 atcgctttta tttcccgaaa gaaatgttag accaaaacag catcttcgca agcatcaccc
     3781 agcaattttt acacttgatt gagaacgatc attttacccc aaataaaaac taaaagagga
     3841 aatttatgag tattaaacta atcgccgtcg atattgacgg aaccctagtc aacagccaaa
     3901 aggaaatcac tcctgaagtc ttttctgcca tccaagatgc caaagaagct ggtgtcaaag
     3961 tcgtgattgc aactggccgc cctatcgcag gcgttgccaa acttctagac gacttgcagt
     4021 tgagagacga gggggactat gtggtaacct tcaacggtgc ccttgtccaa gaaactgcta
     4081 caggacatga gattatcagc gaatccttga cttatgagga ttatctagat atggaatttc
     4141 tcagccgcaa gctcggtgtc cacatgcacg ccattaccaa ggacggtatc tatactgcca
     4201 atcgcaatat cggaaaatac actgtgcacg aatcaaccct cgtcagcatg cctatcttct
     4261 accgtacacc tgaagaaatg gctgacaagg aaattgtcaa atgtatgttc atcgatgaac
     4321 cagaaatcct cgatgctacg attgaaaaaa ttccagcaga attttacgag cgctactcca
     4381 tcaacaaatc tgctcctttc tacctcgaac tccttacaaa gaatgtagac aagggttcag
     4441 ccattactca cttggctgaa aaactcggat tgaccaaaga tgaaaccatg gcaatcggtg
     4501 acgaagaaaa tgaccgtgcc atgctggaag tcgttggaaa ccccgttgtc atggaaaatg
     4561 gaaatccaga aatcaaaaaa atcgccaaat acatcactaa aacaaatgac gaatccggcg
     4621 ttgcccacgc catccgaaca tgggtactgt aaaaggaaag aatagactaa aaccgctcag
     4681 ctgagcggtt tttgtatgaa tatatcacaa tgagagtaaa ttaataaatt taattgcagt
     4741 tgtacagtta cttaaatctt gagagtacaa aaaataggca ttgctt
//
LOCUS       DAVLYR010000124         4675 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLYR010000124 DAVLYR010000000
VERSION     DAVLYR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.124"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            376..1571
                     /locus_tag="VYO63_001647"
                     /pseudo
     CDS             376..1571
                     /locus_tag="VYO63_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1950..2735)
                     /locus_tag="VYO63_001648"
     CDS             complement(1950..2735)
                     /locus_tag="VYO63_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672534.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(2737..3554)
                     /locus_tag="VYO63_001649"
                     /pseudo
     CDS             complement(2737..3554)
                     /locus_tag="VYO63_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            complement(3556..4548)
                     /locus_tag="VYO63_001650"
                     /pseudo
     CDS             complement(3556..4548)
                     /locus_tag="VYO63_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT         1667 a          931 c          771 g         1306 t
ORIGIN      
        1 ctaaaaaaag attttggaat aaactttttc atagcgataa aagcaccgta caagctagga
       61 aaataataag taatcgccca atctgacctt ctccagaact tgattattcg tctatcacta
      121 cagttgacaa acatactaag attagtagtg aggaaatctc caacgggaga gagtactcat
      181 tactttttct ttgtgttaaa atagtggtgt cttgttaagt catagagctc tctatggtgc
      241 tagacgtcgc ttgacaaact ggcaagaaac ctgttttagg aagaatttta tcaaagtatg
      301 tgggatacta gacgtcgagt attggaaata aaatcactaa aacaaggagt cattcaagac
      361 aaaggggtaa acatgatgcg gacagttttc agaatggatg tgagtaaggc aagctcagaa
      421 gtcgctattc ttgtgaatgg tgaaaaggtt catggctata ccatgcccaa tgatgccatc
      481 ggatttagtc gtcttctaga ggatttgaaa taggtaaata cacctgagat tatcttcgag
      541 gcacaggggt ctattctcgt cttcaggcct ttctggagga caatagctat gcttatatac
      601 ggctaaatcc tttaaaacta agaagcaact agacagtttg cgagtccgta aaaccaatca
      661 gattgacgct gaaaagctag cttcgtcaca attcatcctt aaccgcaaac caacttatgt
      721 tcaagaagaa gtctatcaaa atttgcgtga cctcagtcga ttctatcaga atctgacaga
      781 agacatcgtt aggactaaga atagcctgca caaggtgctt caaattgcct ttccagaaat
      841 aaagaacctt ctctctactc ctacagggga acaatattgg aacctcgtga ccatctttcc
      901 aaccaagcat tggaggctga aattatcagt agttgagcta aaagaagcca tcgtaattca
      961 acttctaaac ggatttctga gaacagagtt ggacgattgg ctgaaaagtt gagcgagctg
     1021 gccgaacagt cctatcttgc catttcaaaa acctctccca tgacagaaga ggttaagtat
     1081 catgcacagg aactgttgag actgacaaac cgcaaggatc aggacttagc acaaatgatt
     1141 gaattggctg aaccattacc tgaatacgag attctgctct ctattcctgg tatcgcagaa
     1201 acaacagcta caagcattat tggcgaattg gagacattcg tcgctttcgg tctgccaacc
     1261 aaatcaatgc ctttatcagt attgacctca gacactatga atctggcaat ttcctagctc
     1321 aagaacatat caccaagcgt ggaaatcctt acgcgagaaa aattctctat aagtgcgtcc
     1381 acaatattgc ttcagctagt cataccaatc cttgtcatat cgcggacttt tatgagaaac
     1441 gaaaaagaca atcgcagacg acttcaacca agccatatac gattgtctct atacatcgcc
     1501 tcatccggac aatgtattac ctcataacgc ataacaaact ttacgactac actttaaccc
     1561 aaagtcatta aagtcatcta tgtcctatca ttgtaacacc ttatttttta ataccagata
     1621 agtgtctttt aagtgtgccc aaatttcaaa aaacagctcg caattatctt ctaaacattc
     1681 ctttgatact cattcagaaa accttttgag ctagaatatc gtatgtccct taacaaatgg
     1741 tatgtataaa agagctaaaa aaccactcat gatatgaacg agtggttatg actgaaatat
     1801 tacatacctt tgtatcattt ttgcacaatg gtcaaatttc ttgtttgaaa aactacagta
     1861 agcctaatca aagctctcaa tgtatcatta aaacttcctt ccaaagaata ggaagttcta
     1921 tcaaaattct taattagatt ttacttcttc taagaccctg cgctctcata tgcatccaaa
     1981 cctttgttcc ataattttaa tgataaggta aaaaatatta atgagataag gatcaatcca
     2041 ccaatattga acaatccatc cttgtctttc aagaaatagc tagctgggta ataggctgta
     2101 aaagcaaaag gaatgataaa actaatcaac caacgtaaaa acgaatggta aatcgcaatc
     2161 gggtattttg caaaatcatt aaacatgtaa aaaatataaa ttattgcccc agattgttta
     2221 gtccaaaaag caatactagc tgttacaatt tttaaagagg tataaattaa ggttgcaaag
     2281 ggaatactga ttaaaaagag aaaaaccttt gcccaagtcc aagtaagact agttattgtc
     2341 attaacagca ataagacgcc tactaacaat tctccaagag catcaatttg aaacgtttct
     2401 actaaaatat gaaacaaagg gctaatgggt ctagtcaaat acttatcaaa ttctccttta
     2461 cgaattaaat gttgtcctaa agcccacaga ttatcaaaaa agagatggtc aattccctta
     2521 ggaatgagag aaaaaccata aataaaagca acctgatgaa aactccagcc atcaagtaag
     2581 ggaatatgtt gaaacaaaat atttagaaac agcatgttta acccttgtgt caaaaagaca
     2641 cccacaacac caactaaaaa atcagcctta tactccataa tctgtttaag atattgtcgg
     2701 ataaaaatca ggtgcatgcg ttgatatttc ttcattctaa cctccttgaa tagttatata
     2761 attctgtacg cacttccaaa tcagttggga taaggcaatc ataataaaca gccagaataa
     2821 ttgtagcgag agcgcctgaa tcatttgact catgctatac ttttctataa taaccatcac
     2881 cggggtatag attaaagaag aaaatggcaa aaaagataat agttcggcaa taatttttgg
     2941 aaaaaatgtc agaggaacca aacttccaga taaaaaggca actaaagaat ttttcaaaag
     3001 atttgatccc cataagtttt taaacacaaa agctgaaaaa ccaaagcaaa tattgaaaaa
     3061 aaattaatca aaaaagctaa gattaagcta agaaggtaaa ctgtggttat taaaacaatt
     3121 tgtaagaagg actcacctga taagagcttt agacctgcaa tcagaattac aaatggtaga
     3181 ccaacactaa caaaaactag ccatctagac ccaatttctg tgaaaagata agacattgca
     3241 aaatgaactg gacgaagcag tcgcataata atagagccat cctttacttc ccatccaatc
     3301 ataaaagaac tatctgattt tgtcagtaga ttcgtcacaa aactcatgat gatatataag
     3361 gtcatatcag ataaagtaaa accttgaatg agagactggt gagatgaatc aaacacagct
     3421 ttccacaagt aaaatgctac aaaggccccc atcacatccc caatgcgata aagaaaaaaa
     3481 ttgacacgat acgtgattaa ttcttggatt ccagcgctaa caaaaggctt atagcgtttc
     3541 cataatttag tcatcctaaa gctcctttcg atagaagcga cgaataatat cttcaatatt
     3601 cgcatccgtc atctttaaat cacggatcgt aaaatcagac aaagtttgtt gaataatgtc
     3661 agccgtttga tactgcgagc tgtcgtactg aatatcaagt gacaattcct gtctcgtcac
     3721 atagatatca gaaagtcctt caaaatgaga gacaatataa tcctgcccag gatgtagttc
     3781 aaaagagaga gtcttcatct tcccgaaagt ctttttcagt tgattcacag ttccatcaaa
     3841 gatttctcgg cccttatcaa tcataaaaat acgatcacat aactgttcaa tatcacctaa
     3901 atcgtgggtc gttaaaagta tggtcgtctc ttcttcttga ttaatctgtg taatggcacg
     3961 tcgaatatta tccttgaccg aaacatctaa accaatagta ggctcatcta ggaaaagaac
     4021 tttcggatta tgcaataagg aggccgcaat atccgccctc atacgttgac ctagagatag
     4081 agttctgaca ggatcttgaa taaattcctt caaatctaaa acttcattta gaaaatccat
     4141 gcgtttttga aagaccgcat caggaacatc ataaatttcc tttaaaaccg agtaagtctc
     4201 ctacaaagcc aaatcccacc aaagttgagt tcgctgccca aaaactacac caatatcttt
     4261 aacatagcct tgacggttct cttgaggaat ctttccattg atacgacaaa aacctgatgt
     4321 tggtttcaaa atccccgtca acatcttgat agttgttgat ttccccgctc catttgcgcc
     4381 aataaagcct aaaatttgtc ctttgggcac ctcaaatgat aaatctttaa cagcttcaaa
     4441 gacttgtctc ttgggatgaa aaaaagagcg aaaagctccc tttaagccag gttcctttat
     4501 tgttttcaca aaatttttct gaagatgctc tactgttatc atttttataa atcactcctt
     4561 tcgtttcact caagttataa tatcaacaaa aattgtagca tactacagta aagttaagaa
     4621 aatttaccat cattacaaac catatgaaaa aagctactaa aactatgcca tcttg
//
LOCUS       DAVLYR010000125         4665 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLYR010000125 DAVLYR010000000
VERSION     DAVLYR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.125"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            236..2569
                     /locus_tag="VYO63_001651"
     CDS             236..2569
                     /locus_tag="VYO63_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1672535.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2582..4045
                     /locus_tag="VYO63_001652"
     CDS             2582..4045
                     /locus_tag="VYO63_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1672536.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFKDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4045..>4665
                     /locus_tag="VYO63_001653"
     CDS             4045..>4665
                     /locus_tag="VYO63_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1672537.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKS"
BASE COUNT         1638 a          696 c          956 g         1375 t
ORIGIN      
        1 atagctcacc agcccgactg aaagtgctta tagaatgata ataagtcgcc tgccgaaaac
       61 agcggaaaat agcggtgtta tgcggagata atctgacgcg atgcgaaagt atattgcata
      121 cttattttca acaatttagc agagtatttt tataagtgtg atataataga agtataattt
      181 gttctgatag tttattttat ggagaagtag atttttagaa tgcggagggt tcaatatggt
      241 tgagtttata aagtctaaga aagaaatgag tgaggaggat attaaagcaa atttcatcac
      301 tcctgctatt gtatccaaag gatggaaaaa tggtgagcat atcgcttacg aagaatactt
      361 cactgatggt cgaattgagg ttagaggaga taaggctcgt cgtaaagaag gaaaaaaatc
      421 agactattca ctgtattacc aatttggaac tcgaattgca attgttgagg caaaggataa
      481 taaacacagc gttcgagcag gattacaaca agctattgaa tatggagaga ttttagatgt
      541 tccatttgtt tattcttcga atggtgatgg ctttattgaa cacgaccgta tcacgagaga
      601 agaacgtgag ctggagttag acgaattccc tactcgtgaa gaattatttt ctcgtatgac
      661 gaaggaaaaa ggattgacgt acgaaattac agaagctatc tcaactccat actatacaga
      721 cgccttctca atgaaaacgc cacgctatta tcagcaaata gctatcaacc gtactattga
      781 aacagttgcc agaggacaaa aacgagtaat gtttgtgatg gcaacaggaa cggggaaaac
      841 gttcatggct tttcaaatta ttcatcgcct tcgaaaagct ggtttggcta aacgagtttt
      901 attcttagcg gatagaaaca tcttagtaga ccaaacgatg gctgaagact ttaggccatt
      961 cgaaaaggta atgacgaaaa ttacaccaaa acttttgact gctcctgaaa aattaaattc
     1021 ttttgaaatt tatctagggc tttatcagca actaactggt gaagatggaa ctgaaacaca
     1081 ttatcaaaaa tttgacaaag acttctttga tttaatcgta attgatgaag cgcaccgtgg
     1141 ttcagctaag gaaaacagta actggcgtaa gataattgat tatttcagtt ctgcgacaca
     1201 gattgggatg accgctactc ctaaagaaac caagaatgct tccaatacgg aatactttgg
     1261 tgagccaatc tatacttata gtttaaaaca gggaatcgag gatggttttt tggctccata
     1321 tcgtgttatg agagttaatt tagatgtaga tgtggatggt tatcgtccag aaactggaaa
     1381 agttgatgct aacggacaat taatagaaga taggtactac ggcaggaaag attttgataa
     1441 aaccattgtc attgatgata gaacgcaaag agttgccaag tttgtttctg attatatgaa
     1501 gcaaaacaat gcacgatttg ataaaacaat tgttttttgt gttgatattg accatgccga
     1561 gcgaatgcgt gctgcacttg taaaagagaa tctagactta gtccaagaag actatcgtta
     1621 tgtcatgcaa gtaactggtg acaacgctga aggaaaagct caactggata actttatgga
     1681 tgtcaattct aattttcccg ctattgtaac aacgtctaaa ttattaacga caggagttaa
     1741 tgctaaaaca tgtcgtttga ttgttttaga ctctaatatc caatccatga ctgaatttaa
     1801 acaaattatt ggtcgtggca cacgtcttta tcctcaaaag gggaaagaat tttttacgat
     1861 tattgatttt cgaaatgtta ccaatttgtt tgctgaccct gattttgatg gtgatccagt
     1921 gaaggtgctg gaaacaggtg cgaaaacagt cagtggttct acgcccggtt tcgtaagtga
     1981 ggaaggtgac ccagtagaaa aatatatcgt tacagacaag caggttacca ttcttaattc
     2041 tactgttcaa gtattggatg aaaacgggaa actgattacc gaaagcctga ccgactacac
     2101 tcggaagaat atcttaggta gctacgccac tttgaacgat tttatcacag tttggcatac
     2161 ggcggataaa aagaagctta tcttagacga actttataaa aaaggagttt atctagatgc
     2221 tattcgagag tcggagggaa tatcagaaca agaaatcgat gattttgatt tactcctaaa
     2281 acttgcctat ggtcaaaaag aattaaccaa aacggaacgt atcaataagc tcaaacaaag
     2341 cggatattta tataaatata gtgaggaagc gcgtgctgtt ttggaaattt tactgaacaa
     2401 atacatggat aaaggtattg gagaactcga aagcattgaa acattaaaac ttccagaatt
     2461 tcagatatat ggtggaacct tcaaaatcat caatacttat tttggagata aaaaacgata
     2521 tttacaagca attaaagaat tggagcaaga gctatttaca gtagcttaat gaaaggaaag
     2581 tatgtcaatt acatcatttg taaaaagaat tcaagatatc actcgaaacg atgctggtgt
     2641 taatggtgat gctcaacgta ttgagcaaat gtcttggtta ttattcttaa aaatttatga
     2701 tagccgtgaa atggtttggg aattagaaga agacgagtat gagtcaatta tcccagagga
     2761 attaaaatgg cgaaattggg ctcatgctca aaatggggaa cgggtattga caggcgatga
     2821 attacttgat tttgtcaata acaagttatt caaagagttg aaagagcttg aaataacttc
     2881 aaatatgcct attcgaaaaa cgattgttaa atcagctttt aaagatgcga acaactatat
     2941 gaaaaatggc gtcttgttac gccaagtcat caatgttatt gatgaagttg atttcaatag
     3001 ccctgaagat cgtcattcgt ttaatgatat ttacgaaaaa attcttaaag atattcaaaa
     3061 tgctgggaac tcaggagaat tttatacacc acgtgcagcg actgatttta ttgccgaagt
     3121 tcttgaccca aaacttggag aatcaatggc agaccttgct tgcggaacag gaggcttctt
     3181 gacttcgact ctgaaccgtt taagtagtca acgtaaaact agtgaagata ccaaaaaata
     3241 taatacagct gtttttggta ttgaaaagaa agcatttcct catcttttag cagttacaaa
     3301 tctgtttctt cacgaaattg atgaccctaa aattgttcat ggaaatactt tagagaaaaa
     3361 tgttcgtgaa tatacggatg atgaaaaatt tgacattatt atgatgaatc caccttttgg
     3421 agggtcagaa ttagaaacaa taaaaaataa ctttccagca gaattacgga gttctgaaac
     3481 agctgattta tttatggctg tcattatgta tcgtttgaaa gaaaatggtc gtgttggagt
     3541 tattttacct gatggttttc tatttggtga aggtgtaaaa actcgcttga aacaaaaact
     3601 ggtagatgag ttcaacttgc atacgattat taggttgcct catagtgtct ttgcaccgta
     3661 tacaggaatc catacgaaca ttcttttctt tgataaaaca aagaaaacag aagaaacttg
     3721 gttttatcgt ttagatatgc cagatggtta taaaaatttc tcgaaaacta agccgatgaa
     3781 gtcagaacac ttcaatcctg ttcgtgactg gtgggaaaat cgtgaagaga ttctggaagg
     3841 taagttctac aaatctaaat catttacacc tagtgaattg gctgagttga attataattt
     3901 agaccagtgt ggttttccaa aagaggaaga ggaaatctta aatccctttg agttgattca
     3961 gaattatcaa gcggaaagag caactttaaa tcataagatt gataatgtat tagctgatat
     4021 tttgcagttg ttggaggaca aataatgaca ccagaacaac ttaaggcaag tattctccaa
     4081 agagcgatgg aagggaaatt agtgccgcaa aatcccaatg acgaacctgc aagtgaatta
     4141 ttaaagagaa ttaaagctga aaaagaaaaa cttatcagtg aaggaaaaat caaacgagat
     4201 aaaaaggaaa ctgagatatt tcgtggtgat gatgggaaac attatgggaa gtttgctgat
     4261 ggaagtactc aagaaattga tgttccttat gatattcctg atacttggga gtgggtgagg
     4321 ataaaatcaa tttattggaa ttttgggcaa aataagccag agaaatcctt taggtatata
     4381 gatacgtcta gtattgatag aaaaaagaac ataatcaact acaaaaatct acaatatctt
     4441 tcacctgaac aagcgccttc ccgtgctaga aaattagttt cgcagaatag tgtcttattt
     4501 tcaacagtta gaccatatct aaaaaatatt gctgtagtta gagaacttaa agagtatttg
     4561 atagctagta cagcatttat tgttttggat actttactta acgaaacata tttgaaatat
     4621 tatttattat cagataattt tatcaatcgt gttaataata aatct
//
LOCUS       DAVLYR010000126         4656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLYR010000126 DAVLYR010000000
VERSION     DAVLYR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.126"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(75..146)
                     /locus_tag="VYO63_001654"
     tRNA            complement(75..146)
                     /locus_tag="VYO63_001654"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:cct)
     gene            complement(188..535)
                     /gene="rplS"
                     /locus_tag="VYO63_001655"
     CDS             complement(188..535)
                     /gene="rplS"
                     /locus_tag="VYO63_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1672538.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(654..983)
                     /gene="crcB"
                     /locus_tag="VYO63_001656"
     CDS             complement(654..983)
                     /gene="crcB"
                     /locus_tag="VYO63_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1672539.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(977..1351)
                     /gene="crcB"
                     /locus_tag="VYO63_001657"
     CDS             complement(977..1351)
                     /gene="crcB"
                     /locus_tag="VYO63_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1672540.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1348..1614)
                     /locus_tag="VYO63_001658"
     CDS             complement(1348..1614)
                     /locus_tag="VYO63_001658"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1672541.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1730..2173)
                     /locus_tag="VYO63_001659"
     CDS             complement(1730..2173)
                     /locus_tag="VYO63_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1672542.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSIGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            2277..3212
                     /locus_tag="VYO63_001660"
     CDS             2277..3212
                     /locus_tag="VYO63_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1672543.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3266..3550
                     /locus_tag="VYO63_001661"
     CDS             3266..3550
                     /locus_tag="VYO63_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1672544.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3607..3825)
                     /locus_tag="VYO63_001662"
     CDS             complement(3607..3825)
                     /locus_tag="VYO63_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672545.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETKPTDESGT"
     gene            complement(3822..4656)
                     /locus_tag="VYO63_001663"
                     /pseudo
     CDS             complement(3822..4656)
                     /locus_tag="VYO63_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1431 a         1026 c          857 g         1342 t
ORIGIN      
        1 aaaaatgaac ttgagaaaat tgccttcaaa taaacatttt gaattttctt tgtctttctt
       61 tacattacta aaaatgcccc ctgcaggaat cgaacctgca actactcctt aggagggagt
      121 tgttatatcc attgaactaa gggagctaga taaaaactct gctgaaaagc agagtttttt
      181 agtcgaatta acgacggatt tctttgatac gagctgcttt accttgaaga gcacgcaagt
      241 agtacaattt cgcacgacgt actttaccgt aacgaacaac ttcgattttt tcaacacgtg
      301 gagtgtggat tgggaagata cgctcaacac ctacaccgtt agagatttta cgaactgtgt
      361 agttttctga gattccagca cctttacgtg cgataacaac accttcaaaa atctggatac
      421 gttcacggtt accttcgaca actttcgcgt gtacacgaac agtgtcacca ggacggaatg
      481 atgggatatc tgtacgaagt tgaccttcag tcaagctttg gattaatgga ttcattttat
      541 tctcctatct ttgtcaatct tgaggaaatt tcctcagcgg ataaactgta tttttgtgcg
      601 tccatcacac acaagataca gtctaccaaa ttttcacaaa aaagtaaaga aatttatagc
      661 aaaattccta aaaaaatcgc aaccaaacca cctatgtaag tcaaagtcag gtaagaataa
      721 aagaccttct tatcacttaa cagtctttga agttcgtcat tcaaggtcga aaaagttgtt
      781 aaaccgccac aaaatcctgt tgctagaata gcatatactt ccttagactc tacatgattg
      841 tagaatactc caattaaaaa acaacctaaa agattagcta tgagcgttcc gagtggcaat
      901 ttagaagctt gattgtagcg ggaaaagaaa taccgtacga gtgctcccaa gccacaggcg
      961 attgcaagat agacgattac catttcttcc tccctagaaa ataagctaag agtaggcctc
     1021 caccgatgct caaaagcaaa tacaggacta aactaagata acgccctgta tcaagcagct
     1081 tcacagtatc aagcattagg ctggaaaaag ttgttaaacc tccgcaaaaa cccgtcccca
     1141 gtgctaaaat caagccctta ctagtcccct tatagaccag atagcccttg acaagaaaaa
     1201 tcaagcagaa aattcccaga tagttgacaa agagagtgcc ccaaggaaag tctggactgg
     1261 ctggtaacca ggtggaaact agatatcgga caagtccgcc caacatagca gctagaaaaa
     1321 ttcctagcgg ataaaattgt tcttttttca tttgatattt tgatcctgat aatcacgcga
     1381 acgtttgagt atgtccgaaa aagtcgctac aatagtctcc tgatagcgct tgtcctctac
     1441 acgactccta accttttcaa aaataacttc ttctctctta gtatctaaaa tcggtttacc
     1501 tgaagctttc ttataagcaa ccaccccctc aactaaatgc attcgttctt ctaacagttt
     1561 gacaatttgg tcatcgattt gatcgatttc ttgacgaata atatctaaat ccatacagtc
     1621 tcctccttta tttgaattat tgtatcaaaa agccatcaat taggctagta aaatcccaac
     1681 tctcgataag aaaaatgcac tgattgatta catcagcgca cttttatgct tatactactt
     1741 tagcaaccaa ttcttctgcg aattgttcca agcgttcaat atcttcttcc tcggcagaaa
     1801 gatcaacttt aacacactct gaaccttttt ctgctcctat tgacacaaag acacggtcaa
     1861 agtcatcaac agccttacag aattcatcgt agaaggtatc tcctgagccg accactccgt
     1921 agattttgcc attcaagttg agatctgcta ggtcttcgta gaagtccatc atctcatctg
     1981 gcaattcacc atcaccataa gtataggtcg caacgatagc gatgtctgct tccaagaagt
     2041 ctgaagcgtc aacagttgta cattcatcaa catcaacatc caagcccaag tcacgtaatt
     2101 tgtctgctac aatatctgca atttcttcgg tattaccggt catactggca aatacaattt
     2161 ttgctaatgc catatcgtcc tcctcaattt atctttctcc attatatcac atctttttca
     2221 ttttaaaaat aaaaattctt gtgctacaat gaaatgagaa agaattgagg ttatttatgg
     2281 agatttgcca acaaatttta gaaaaaatta aagaatacga caccattatc attcatcgtc
     2341 atatgaaacc agaccctgat gccttgggaa gtcaggtggg attgaaagcc ttgctggaac
     2401 atcatttccc agaaaaaacc atcaaagccg tcggttttga tgaaccaact cttacttgga
     2461 tggctgagat ggatcttgtt gaagatagag cctaccaagg cgcacttgtc atcgtctgtg
     2521 atacagctaa tactgctcgt atcgatgata agcgctatag tcaaggtgat tttctcatta
     2581 agattgacca ccatccaaat gatgatgtat acggtgacct gtcttgggtc gatactagtt
     2641 caagtagcgc tagcgagatg attaccctat ttgcccaaac aacccaacta gccttggcag
     2701 atcgcgatgc tgagttgctc tttgcaggaa ttgtcggtga tacaggtcgc ttcctctacc
     2761 cttctaccac tgcacggact cttcgcctgg ctgcttattt gagagaacat aactttgact
     2821 ttgcggctct cactcgcaaa atggacacta tgagctacaa aattgctaaa ctgcaaggct
     2881 acatctacga ccatctggaa gtggatgaaa atggtgctgc tcgcgttatc ctgagtcaag
     2941 aagtcttgaa acaatacaat gtaaccgatg ctgaaactgc agccattgtc ggtgcacctg
     3001 gacgcattga cagagtgagt ctctggggaa tttttgtcga acaggctgat ggccactacc
     3061 gagttcgctt acgcagtaaa atccatccta tcaatgaaat cgccaaggag catgatggtg
     3121 gaggccaccc tctagcaagt ggtgctaatt cctatagcct agaagaaaac gaaatcatct
     3181 accaaaagtt aaaaaacttg cttaaaaact gataaaatac ttgccaaact tttcagaatc
     3241 tgatagacta gtatggtaac aatctatggc tcgcaaagag accatggcag aaaggaaata
     3301 ttgcaaaatg aaaaaagata tccatccaga atatcgccca gttgtcttca tggacacaac
     3361 tactggttac caattcctta gcggttcaac aaaacgctct aacgaaacag ttgagttcga
     3421 aggcgaaact tacccattga tccgtgtgga aatttcatca gactcacacc cattctacac
     3481 tggacgtcaa aagttcactc aagcagatgg acgcgtggat cgtttcaaca aaaaatacgg
     3541 tctcaaataa tgataagaga acagtttcgg ctgttctttt ttgtttcttg aaatcaactg
     3601 ctgttttcat gttccagact catctgtagg tttggtttcc atgctactag gcaggaagga
     3661 aatagctgtt tcaacacgtc cataatgagc tgtactattg tcacgaacca cactttcatt
     3721 gatggtccaa gtggaattca ttttcttaaa agcttctcgg actttttcca aatctttgga
     3781 ggcaatggcc tgctctaagg tttcaaaacg aggacttata ctcatctgct ttcacaaagc
     3841 attctagtcc atctccgatt aacgatgggc tttatcacct ccttctccag tccttgtatg
     3901 atatcttaaa gttgattcat gacatcttcc aaagtttgaa aggctttatt tttaaatcca
     3961 cgtttacgaa tctctttcca cacttgttca atggggttca tctctggtgt gtacggagga
     4021 ataaatgcaa agccaatatt agtcggaatc tttaaggtac ttgatttatg ccatatagca
     4081 ttgtccataa cgagtaaaag ataatcatct ggataagctt gtgaaagctc ttctaaaaag
     4141 gcgttcatcc actcagtatt acatccacca gctcttaaga aaaatgattc gcctgtatgg
     4201 gcatcaacag ctccataaca atagcgaaat tctcgtatat agtgactatg gacatgtgga
     4261 cctactccta ttggagacca acaagatccc agtttactga ttctaccgaa accagcctca
     4321 tcttggtaca tcgagcgaac cttatgaaaa ggtctactgg ttttaaagcg ctttcttttc
     4381 ttcttgaatt gagattttat ttttagacgc gacaatggtt tgagcgtctg ctttcttagg
     4441 atgttctgga cgtggcataa tatttcgcca accatggcgc ttcaacagtt gatagaaggc
     4501 atcacgtgtg taggaacgat ataagcctga aataaggtat tgtaacaaat tctcctgcct
     4561 ctgtagcctt caaatggcgg gcaagaaagg ctttctcttc ctcaactgtc atatatgcat
     4621 ggttacgacc accacgtgtt tcttgaagga gagagt
//
LOCUS       DAVLYR010000127         4630 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLYR010000127 DAVLYR010000000
VERSION     DAVLYR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.127"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            50..679
                     /locus_tag="VYO63_001664"
     CDS             50..679
                     /locus_tag="VYO63_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1672546.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQTGNVVFLSVELIKNNVSDVR
                     DKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIAFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLSMLFASWQKKVKEKA"
     misc_feature    744..831
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            867..1181
                     /gene="rplU"
                     /locus_tag="VYO63_001665"
     CDS             867..1181
                     /gene="rplU"
                     /locus_tag="VYO63_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1672547.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1197..1541
                     /locus_tag="VYO63_001666"
     CDS             1197..1541
                     /locus_tag="VYO63_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1672548.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1558..1851
                     /gene="rpmA"
                     /locus_tag="VYO63_001667"
     CDS             1558..1851
                     /gene="rpmA"
                     /locus_tag="VYO63_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1672549.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2161..2369)
                     /locus_tag="VYO63_001668"
                     /pseudo
     CDS             complement(2161..2369)
                     /locus_tag="VYO63_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2715..3632
                     /locus_tag="VYO63_001669"
     CDS             2715..3632
                     /locus_tag="VYO63_001669"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1672550.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3725..4573)
                     /locus_tag="VYO63_001670"
     CDS             complement(3725..4573)
                     /locus_tag="VYO63_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1672551.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT         1474 a          801 c          938 g         1417 t
ORIGIN      
        1 attacctata agaaaaatat cacgtcagtc taaaaggtta gcactttttt tgacgttttg
       61 tgctggatat gtggatgctt acacttttat tgttcgcggg aatacccttg tagctggaca
      121 aactggaaat gttgtctttc tttcagtaga attaattaaa aataatgttt cggatgttag
      181 ggacaaggtt ctcaccttgc tagcgtttat gatgggagtc tttttattaa cgatttataa
      241 ggaaaaattg agaattgtga aaaaacctat tctgtccttg attcccttgg caatcttatc
      301 aatcattatt gcttttgtgc cgcaaactgt ggataatatc tatctagtgc cgcccttggc
      361 cttctgtatg ggactggtga caactgcttt tggagaagtg tcgggtattg cctataataa
      421 cgcttttatg acagggaata tcaaacggac catgctggct tttggagatt atttccgaac
      481 caagcacact ccttttttgc gtgaaggatt catatttgtt agcctgctta gtagttttgt
      541 ccttggcgtt gtcttttcag cctatttgac gattttctat catgaaaaga ccattcttgg
      601 tgttcctatt atgatgagcg ttttttacct cagcatgctt tttgcctctt ggcagaaaaa
      661 agtaaaagaa aaagcttgat tttaggtgtt attacttgta agaaaatagg agatatgcta
      721 tacttgaaga gttgactatg cacgatcctg tgcaaccgca cgaacatcgt tgcttgatta
      781 ttcaagttta agtggttcgt cccacgatgc taggcgagtc ttcacaaaaa atcggggaaa
      841 cccataaaaa tcataggagg tgcataatga gcacatacgc aattatcaaa actggcggaa
      901 aacaagttaa agttgaagtt ggtcaagcag tttacgttga aaaattgaac gttgaagctg
      961 gtcaagaagt tacttttaac gaagttgttc ttgttggtgg tgaaaacact gttgtcggaa
     1021 ctccacttgt tgctggagct actgtagttg gaactgttga aaaacaagga aaacaaaaga
     1081 aagtggttac ttacaagtac aaacctaaaa aaggtagcca ccgtaaacaa ggtcaccgtc
     1141 aaccatatac aaaagttgtc atcaacgcaa tcaacgctta attttaagga gaacacatga
     1201 tacaggcagt ctttgagaga gccgaagatg gcgagctgag gagtgcggaa attactggac
     1261 acgccgagag tggcgaatac ggcttagatg tcgtgtgtgc atcggtttct acgcttgcca
     1321 ttaactttat caattctatt gagaaatttg caggctatga accaatccta gaattaaacg
     1381 aagatgaagg tggctatctg atggttgaaa taccaaaaga tcttccttca caccagagag
     1441 aaatgaccca gttattcttt gaatcatttt tcttaggtat ggcaaactta tcggagaact
     1501 attctgagtt cgtccaaacc agagttatca cagaaaacta acacggagga aaacattatg
     1561 ttaaaaatga ctcttaacaa cttgcaactt ttcgcccaca aaaaaggtgg aggttctaca
     1621 tcaaacggac gtgattcaca agcaaaacgt cttggagcta aagcagctga cggacaaact
     1681 gtaacaggtg gatcaatcct ttaccgtcaa cgtggtacac acatctatcc aggtgtaaac
     1741 gttggtcgtg gtggagacga tactttgttc gctaaagttg aaggcgtagt acgctttgaa
     1801 cgtaaaggac gcgataaaaa acaagtttct gtttacccaa tcgctaaata aaaaggtcca
     1861 ttgaaccttt tatcccgaac cttgaaatgt aaaggtgagg aagctagaaa cagcttaaaa
     1921 taaggctttc cgagttttcg gagagcctgt tttttatttt gatacccata ctttttaata
     1981 tgtatagtgg attgatactc ttcgaaaatc aaattcaaac cacgtcaatt tcaccttgcc
     2041 gtactcaagt acagcctgcg gctaacttcc tagtttgcac tttaattttc attgagtatg
     2101 aatctggaat agaacactgt taaaaattga tatgagtttc ctactcgatt tcttcagatt
     2161 ttatttcaat tgactataag aactgctatc tggtttcaaa tattggcttg tcgtcccttc
     2221 acttgtctga atggcttgag ccgttccttc tgataaggga aaagatattc tttagctgaa
     2281 acggtctgag aatccaagct aacagatgga gagaaaaata gcctagtctg aagcacacta
     2341 gatgggagtt taggaaattc ttcaaacatc ttgcccaagt tgtctcattt tgtaaatttt
     2401 acagccatag gtgtacctcg attctaaatt ctaccttttc ggcaccaatt agtaatttaa
     2461 agttctcagg agttagttgc attgcttttt catacataac atagtatgac actttatggt
     2521 taccgaacaa gtctaatcac tccttgcaaa attttgaaga tgtcctacaa gtttttgaga
     2581 agaatattgc aaagttcata gttaagcaac gatagaataa agtgcttttt gaaaacaggt
     2641 acacttcatg tgaaaaaaac aacatttcag attttctgtt ccagaaatag ctatcgctat
     2701 gatataatta ttttatgatt attactattc ctataaaaaa ccaaaaagat attggcacac
     2761 catctgattc agtcgttgtt ctcggctatt ttgatggcat acataagggg catcaagaat
     2821 tatttcgtgt tgccaacaag gctgcgagaa aggatttatt acctatcgtc gttatgacct
     2881 ttaatgaatc tccaaagatc gctttagagc cttatcatcc agatttgttt ttgcatattt
     2941 tgaaccctgc tgaacgtgaa agaaaattaa agcgcgaagg tgtagaagaa ttatatctcc
     3001 ttgattttag tagtcaattc gctagtctta ctgcacaaga attctttgca acttatatca
     3061 aggctatgaa tgccaaaatt attgttgcag gttttgatta tacatttggt tctgacaaaa
     3121 aaacagcaga agatttaaag gattactttg atggagaagt tatcattgtt ccacctgtag
     3181 aagatgagaa aggaaagatt agttcaactc gtatccgtca agctatttta gatggaaatg
     3241 tgaaagaagc aggaaaactt ttgggggcac cgcttccatc aagaggtatg gtagttcatg
     3301 gtaatgctcg tggtcgtaca attggttatc cgacagcgaa tttagtgctt ttagaccgta
     3361 cttatatgcc agcagatggc gtttatgtcg ttgatgttga gattcaaaga cagaagtatc
     3421 gtgctatggc tagtgtcggg aaaaatgtga cctttgatgg agaagaagca cgttttgaag
     3481 tcaatatttt tgattttaat caagatattt atggggaaac cgtcatggtt tattggcttg
     3541 atcgcattcg tgatatgacc aaatttgact cagttgacca attagtggat cagttaaagg
     3601 ctgatgaaga agtaactcgg aattggtctt aagagcttga gtaaataaaa caaaaaagag
     3661 gttgtctgta acccaaaaga tagatgattt agtctaactt ttgaggtcac gacattacct
     3721 ctttttattc tttttcaaag gtgaagcctt ctcctaggat ttcatgggct tctgtaatag
     3781 ttataaaggc ttgaggatcg attcgatgaa tcatttcctt cgttttcaca atttcatttc
     3841 ttccgacaat acagtagatg attttcaaat tttctttact atagtagcct tgaccagaaa
     3901 taaaagtaac acctcttccg aggtcatcat taatcgcctt agcaagttgg tcaggacgtt
     3961 ttgtgataat cataaagcct ttgccggcat atcctccttc accaatcaaa tcaataacac
     4021 gagaaacaat aaaatcaaac aaaagcgtgt aggaaaccaa tctcaaatcc ttgaagatta
     4081 ggagaatcaa catgagaata caaaaatcta agataaagag cagttttcct atggatatat
     4141 gagtgtattt gttgagaata cgagctagaa tatcagttcc gccagttgta cctccagcat
     4201 taaaaataat tccaaggcca attcccaata ggattcccgc tataagggct gtgattagta
     4261 aatcaccttg aagatcaata tgaaggggaa tatgctcaaa aaaagctaac caggcggaca
     4321 aagctaaggt tcctagtaaa ctagaataga gggatttggc tccaaagatc ttccaagcta
     4381 ggatgaaaag gggaatatta atcagcaggt tcatgaggga aacagggatt ttaaaaagat
     4441 aaaaggtgat gagggtaatg cctgtcgccc ctccttcaaa gagatgatgg ggaactacaa
     4501 aataagtcag tccaaaagca taaatagcag cacctagtaa aatggtaaaa atggggtaaa
     4561 tttttttaat cataggacac ctcttttctt ataagttgat tatatcaaat tttacggaaa
     4621 atttgattga
//
LOCUS       DAVLYR010000128         4601 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLYR010000128 DAVLYR010000000
VERSION     DAVLYR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.128"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            128..886
                     /locus_tag="VYO63_001671"
     CDS             128..886
                     /locus_tag="VYO63_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1672552.1"
                     /translation="MVLVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDRMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            926..1690
                     /locus_tag="VYO63_001672"
     CDS             926..1690
                     /locus_tag="VYO63_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1672553.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1703..2380
                     /locus_tag="VYO63_001673"
     CDS             1703..2380
                     /locus_tag="VYO63_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1672554.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGATLLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2390..3049
                     /locus_tag="VYO63_001674"
     CDS             2390..3049
                     /locus_tag="VYO63_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1672555.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3307..4497
                     /locus_tag="VYO63_001675"
     CDS             3307..4497
                     /locus_tag="VYO63_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1672556.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         1308 a         1070 c          830 g         1393 t
ORIGIN      
        1 tttgactgtc ccgatcgatt tttcctgttc ttatttcact atagtgaatt ttttcagata
       61 tgatttgaca agaacattct tttattgcag aatcgtgtta taaaatctaa cataaaggag
      121 ttctctcatg gttttagtag aatttaaaaa cgtcgaaaaa tattacggag actaccacgc
      181 actccgcaac atcaatctcc gttttgaaaa aggacaagtt gttgtcctgc ttggaccttc
      241 tggctctggg aagtccactc ttatccgtac gatcaatggt ttagaggctg ttgacaaagg
      301 aagtctccta gtcaatgggc accaagttgc tggtgccagc cagaaagatt tggtacctct
      361 tcgcaaggaa gtcggcatgg tttttcaaca ttttaacctt tatccacaca aaacagtgtt
      421 agaaaacgtg acacttgcgc ccattaaagt tctaggaatt gataaaaaag aagctgaaaa
      481 aaccgccaaa aaatatctgg aatttgtaaa tatgtgggac aagaaagatt cctatcccgc
      541 catgctatct ggtggacaaa aacagcggat cgccatcgct cgtggtcttg ctatgcatcc
      601 ggaactcctc ctctttgatg aaccaacatc tgctcttgat cctgagacta tcggagatgt
      661 tctagcagtt atgcagaaac tggcgcgtga taggatgaac atgatcatcg ttacccacga
      721 aatgggcttt gctcgagagg ttgcggaccg cattatcttt atggccgacg gagaagtttt
      781 agtagatacg acagatgtcg ataacttttt tgacaatcca agcgaacctc gtgcccaaca
      841 attcctcagc aaaattatca accacgaaag tgacaaagtc aaataaggag gcgcctatga
      901 aaaaaaattc tttttatcag cattattgat tagccttttc ggccttgctg ctgccaaacc
      961 agtccaggct gatacaagta tcgcagacat tcaaaaaaga ggcgaactgg ttgtcggtgt
     1021 caaacaagac gttcccaatt ttggttacaa agatcccaag accggtactt attctggtat
     1081 cgaaaccgac ttggccaaga tggtagctga tgaactcaag gtcaagattc gctatgtgcc
     1141 ggttacagca caaacccgcg gcccccttct agacaatgaa caggtcgata tggatatcgc
     1201 gacctttacc atcacggacg aacgcaaaaa actctacaac tttaccagtc cctactacac
     1261 agacgcttct ggatttttgg tcaataaatc tgccaaaatc aaaaagattg aggacctaaa
     1321 cggcaaaacc atcggagtcg cccaaggttc tatcacccaa cgcctaatta ctgaactggg
     1381 taaaaagaaa ggtctgaagt ttaaattcgt cgaacttggt tcctacccag aattgattac
     1441 ttccctgcac gctcatcgta tcgatgcctt ttccgttgac cgctctattc tatctggcta
     1501 cactaataaa cggacagcac tactagatga tagtttcaag ccatctgact acggtattgt
     1561 taccaagaaa tcaaatacag agctcaacga ctatcttgat aacttggtta ctaaatggag
     1621 caaggatggt agtttgcaga aactttatga ccgttacaag ctcaaaccat ctagtcatac
     1681 cgcagattaa ggaggatacc ctatgacaga tttatcatct tggacagcct attttcagga
     1741 ttttggacaa tttttcaatg gtttcctctt tactcttgcc ctagcgattg gatcctttat
     1801 cctcgccatg gtcttaggaa tcttctttgg tgccatgtca accagtaaac gtccaatttt
     1861 acgcatttta gctcgcatct ttgtggaatt ttaccaaaat actcctctct tggtgcagtt
     1921 tgttattgtt ttttatggcc tacctcttat cagtgaccat atcatcatga ttccaattta
     1981 ttggacagcc gttctctgcg tgggactcta tcacggcgct tatatcgctg aggttattcg
     2041 ttcagggatt cagtctattc ctagtggtca gatggaggcc gccttgtcgc aaggttttac
     2101 ctatatcagt gccatgcgct tgattacctt gcctcaggcc ttccgcatca ttctccctcc
     2161 attgaccaac caaattgtta acctcatcaa gaacacctct actgtagcta tcatctctgg
     2221 agtagacttg atgtttgtga ctaagtcttg gtcggctctc aacggaaact atatcccagc
     2281 ctttttaggt gctactcttc tctactttgc cctatgcttc cctgttgccc agtttggtcg
     2341 caagatggag caagccaaca aaaaagccta ttcactttag gaggttacta tggaatctat
     2401 tttagaagtt ttaaccccag ataacctagt ctttatcttt aaaggatttg gcttgaccct
     2461 ctatatttct ctgattgcca tcatcctctc tactatcatc ggtacggtgc tagctgtcat
     2521 gagaaatggc aaaaatcctg tcttgcgaat catctccagc atttatatcg aatttgtgcg
     2581 caacgttccc aaccttctct ggatttttac tatctttttg gtgttcaaaa tgaaatccac
     2641 accagcaggt attacagcct ttactctctt tacatcagca gccttggctg agattattcg
     2701 aggcggtctc aatgccgtag acaagggaca atacgaagca ggaatgtcac aaggcttcac
     2761 ctcagcccaa atcctctact acatcattct cccacaagcc atccgcaaaa tgctaccagc
     2821 catcatttct cagtttgtta ccgtgattaa ggataccagc ctcctctact ctgttatcgc
     2881 cctacaagaa ctctttggag ccagccaaat tctcatgggc cgttatttcg aaccagagca
     2941 ggtcttcagt ctttgcatcc tgattgccct catctacttc agctttaacc tagcaatttc
     3001 tagcctgtct catatgctag ccaaacgttg gcaacaagct gcagaataat actcttcgaa
     3061 aatctcttca aaccacgtta gctctatctg caacctcaaa acagtgtttt gagcaacttg
     3121 cggctagctt cctagtttgc tctttgattt tcattgagta taaacagcca ctctccagtt
     3181 caattggaga gttttttgat gagagtagat atttatagac ctcaatttga caccatacat
     3241 tttctatgat aaaatgtaac aaatttattt acaacaaatc gaaatctaaa tgaaatggaa
     3301 ttttctatga aatcagtcaa aggactactc tttatcatag ctagttttat cttgactctt
     3361 ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt aacaagccta
     3421 tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt tcacagtttg
     3481 gagaaggtct acaccgtcca caaattcaca gcctttctct caatcatcct actaatcttt
     3541 cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcaatt tggcaatctt
     3601 gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata catccaatac
     3661 gaagcttggc gatggattca ccgcctggtt tacctagcct atattttagg actctttcac
     3721 atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt tcttgttggt
     3781 agctatgccc ttttaggctt actagctggt ttttatatca tttttctata tcaaaagatt
     3841 tccttcccct atctagggaa aattacccat ctcaaacgct taaatcacga tactagagaa
     3901 attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc ctttctaaag
     3961 attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg aggtcatggt
     4021 caaactcttt actttactgt taaaaattca ggcgaccata ccaagaatat ctatgataat
     4081 cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat catagaagaa
     4141 ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt catctcttac
     4201 atccgtgaac atcctatttt agataaacag gttcacttct actatagctt ccgtggagat
     4261 gaaaatgcag tctacctaga tttactccgt aactatgctc agaaaaatcc taattttgaa
     4321 ctccatctaa tcgacagtac aaaagacggc tatcttaatt ttgaacaaaa agaagtgccc
     4381 gaacatgcaa ccgtctatat gtgtggtcct atttctatga tgaaggcact tgccaaacag
     4441 attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt caaataagta
     4501 atcatactct cctgggattt cccaggagag ttttttatag ccaaatgaaa taagacagga
     4561 acgatggaat tttggcattc aaatcaattg ataagaatgt t
//
LOCUS       DAVLYR010000129         4496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLYR010000129 DAVLYR010000000
VERSION     DAVLYR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.129"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(86..1072)
                     /locus_tag="VYO63_001676"
     CDS             complement(86..1072)
                     /locus_tag="VYO63_001676"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1672557.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1266..3734
                     /gene="gyrA"
                     /locus_tag="VYO63_001677"
     CDS             1266..3734
                     /gene="gyrA"
                     /locus_tag="VYO63_001677"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1672558.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3734..4477
                     /gene="srtA"
                     /locus_tag="VYO63_001678"
     CDS             3734..4477
                     /gene="srtA"
                     /locus_tag="VYO63_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1672559.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         1366 a          798 c         1013 g         1319 t
ORIGIN      
        1 taatgggggg ctatttcaac ttcaacctac tataatacag aaaaaaacaa ctccctgata
       61 attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat tctgggtttt
      121 tccatgcttc gtcaatgata gcttgcaatt ctttagcaga tgcttgcatt ttttgagttt
      181 ctgcgtcgtt caatgggata tttactggac gaacaatacc atgtgcacca acaacagctg
      241 gttgaccgat aaagacgttc tcaactccgt attgaccttc ttggaatact gaaagtggaa
      301 gtactgcatt ttcatcgtca aggattgctt tagtgatacg agcaagggct actgcgatac
      361 cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga acaccttcga
      421 acaattcaat caattcagct tcttgaacat tttgagtatc tttaaggaat tcttcaaggt
      481 ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg tgttcaccca
      541 tgatgtaggc gtgaactgaa cgagcatcta cgtccaattt ttcagcaagt gcttgacgga
      601 aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg aaaccagaga
      661 atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg aagatacctt
      721 tgaaacctga ttcaacaact tgagttacga ttgatttgtt gatagcaagg tttttaccta
      781 caaggtcaag acgagtttca cctggttttt gaggcgcacc tgcagtgatc acaacaaggt
      841 cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa gtgaaggcaa
      901 gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt ggaatttcga
      961 taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa gatgaaccta
     1021 cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc attgttttaa
     1081 acatctcctt aattttatta ggggattttc cctagacaac ttcattctat cacttttaaa
     1141 aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg gtttagaggc
     1201 tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa atgaggcatt
     1261 tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg aaggcaagtt
     1321 ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt cgagatggct
     1381 taaaacctgt tcaccgtcgc attctttacg gaatgaatga attgggtgtg accccagaca
     1441 aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat cacccacacg
     1501 gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc taccgttaca
     1561 tgcttgtaga tggtcatggg aattttggtt ccatggatgg agatagtgct gccgctcaac
     1621 gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat atcaacaaaa
     1681 atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg gtcttgccag
     1741 cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg atggcaacca
     1801 atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc atggataatc
     1861 ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt ccaactggtg
     1921 ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa ggttcgattg
     1981 tcctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgc atcgttgtaa
     2041 cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt cgcttggttc
     2101 aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt gaaggtgttc
     2161 gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat aacctcttca
     2221 aaatgaccca aatgcaaacc aattttggtt tcaatatgct cgctatccaa aatggtatac
     2281 cgaaaatttt gtctcttcgt cagattttgg atgcttatat cgagcaccaa aaagaagtgg
     2341 ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat atcttagaag
     2401 gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt gctagtgaaa
     2461 cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa cgtcaaagtc
     2521 aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat aagattcaat
     2581 ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt gctaagcctg
     2641 aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa ttttctgata
     2701 agcgccgtac agagttgatg attggacagg tcttgagtct cgaggatgag gacttgattg
     2761 aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt ttggatcagg
     2821 acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga gtgaaagatg
     2881 atgactttgt tcgtgagtta gtgtcaacta gcacccatga tcatctgctc ttcttcacaa
     2941 acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt cggactgcca
     3001 aagggctacc agtagtcaat ctcttgaaat tggatgaaga cgaaagtatt cagacggtta
     3061 tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc cgtcacggta
     3121 ttgtgaagag aactagtgtt aaggagtttg ccaatattcg tcaaaatggt ctcaaagcgc
     3181 tgaatttaaa ggatgaagat gagttaatca atgtcttgtt ggcagaagga gatatggata
     3241 ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc gttcgtggta
     3301 tgagtcgtat cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac acagttgttg
     3361 gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa ggatatggta
     3421 agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga atgcagacag
     3481 ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa ggggatgagg
     3541 atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc aatatttcac
     3601 aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat gctcagatag
     3661 tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa aacgaaacag
     3721 aaggtgaagc ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa aaaatctgct
     3781 gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt ttaatactca
     3841 aattcgaaat attttcatag tctggaatac caataagtat caagttagcc aggtatcaaa
     3901 agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt ctgtcaaagc
     3961 tatctcttcg gaagctgttc taacttctca atgggatgct caaaaattac cagttattgg
     4021 gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac ttgataatgt
     4081 taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag aaggaaatta
     4141 tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt tattttctcc
     4201 tttagataat gctaaaaatg gcatgaagat ttatctaact gataaaaata aagtttatac
     4261 ttatgaaata cgtgaagtca aacgtgtgac accagatcgt gttgatgaag ttgatgatag
     4321 agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta cagaacgtat
     4381 tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg atgaaatcct
     4441 aacagctttc aatcaaccat ataaacaatt ttattaatac aaatcagtga aatcat
//
LOCUS       DAVLYR010000130         4495 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLYR010000130 DAVLYR010000000
VERSION     DAVLYR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.130"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            433..885
                     /locus_tag="VYO63_001679"
     CDS             433..885
                     /locus_tag="VYO63_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1672560.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            904..2241
                     /locus_tag="VYO63_001680"
     CDS             904..2241
                     /locus_tag="VYO63_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1672561.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2320..3204
                     /locus_tag="VYO63_001681"
     CDS             2320..3204
                     /locus_tag="VYO63_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1672562.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3204..4037
                     /locus_tag="VYO63_001682"
     CDS             3204..4037
                     /locus_tag="VYO63_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1672563.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4055..>4495
                     /locus_tag="VYO63_001683"
     CDS             4055..>4495
                     /locus_tag="VYO63_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase"
                     /protein_id="HEV1672564.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLV"
BASE COUNT         1464 a          720 c          892 g         1419 t
ORIGIN      
        1 tagaaaaacg aagtgagcag cttataaatt caagtcccca aatagattca tactagtatc
       61 ttttgcaaaa aataaagggc gacttccttc atgaatatca atttcatcta taaggaaggt
      121 agctaattga actaacttat ttattctgtt tgtcgctaga aaaatcagac ctccttgtga
      181 agattgagga gatactgaat gaaaatcaaa gaagaaacta gcaagctagt agcagattgc
      241 ccaaaacacc gctttgaggt tgtagataag actgacgtat ataatccaag gtgaagcgac
      301 tgtggtttga agagattttc aaagagtata ggctagagag tagtgttttt atgtccttct
      361 agtagaaaat gctagacaga agaatgggga acttggatag gaaaaataga ttgagaaagg
      421 aggttagaag agatgattat tacaaaaatt agccgtttag gaacttatgt gggagtaaat
      481 ccacattttg caacattaat agatttttta gaaaaaacag gactagaaaa tttaacagaa
      541 ggttcgattg ctatcgatgg taatcgattg tttgggaatt gctttactta tctagcagat
      601 ggtcaagcag gggctttctt tgaaacacac caaaaatatt tggatattca tttagttttg
      661 gaaaacgaag aagccatggc tgttacatcg ccggaaaatg taagcgttac ccaagaatat
      721 gatgaagaga aagatattga attatacaca gggaaagtgg aacagttggt tcatttgaga
      781 gctggtgaat gcctcatcac ttttccagaa gatttacatc aacccaaggt tcgtataaat
      841 gatgaacctg tgaaaaaagt tgtctttaaa gttgcgattt cttaatgtag aaagagaaga
      901 acgatgaaaa aaatgagaaa gtttttatgt ctagctggaa ttgcgctagc ggctgttgcc
      961 ttggtagctt gttcaggaaa aaaagaagct acaactagta ctgaaccacc aacagaatta
     1021 tctggtgaga ttacaatgtg gcactccttt actcaaggac cccgtttaga aagtattcaa
     1081 aaatcagcag atgctttcat gcaaaagcat ccaaaaacga aaatcaagat tgaaacattt
     1141 tcttggaatg acttctatac taaatggact acaggtttag caaatggaaa tgtgccagat
     1201 atcagtacag ctcttcctaa ccaagtaatg gaaatggtca actcagatgc tttggttccg
     1261 ctaaatgatt ctatcaagcg tattggacaa gataaattta acgaaactgc cttaaatgaa
     1321 gcaaaaatcg gagatgatta ctactctgtt cctctttatt cacatgcaca agtcatgtgg
     1381 gttagaacag atttgttaaa agaacataat attgaggttc ctaaaacttg ggatcaactc
     1441 tatgaagctt ctaaaaaatt gaaagaagct ggagtttatg gcttgtctgt tccgtttgga
     1501 acaaatgact taatggcaac acgtttcttg aacttctacg tacgtagtgg tggaggaagc
     1561 ctcttaacaa aagatcttaa agcagacttg acaagccaac ttgctcaaga tggtattaaa
     1621 tactgggtta aattgtataa agaaatctca cctcaagatt ctttgaactt taatgtcctt
     1681 caacaagcta ccttgttcta tcaaggaaaa acagcatttg actttaactc tggcttccat
     1741 atcggaggaa ttaatgccaa cagtcctcaa ttgattgatt cgattgatgc ttatcctatt
     1801 ccaaaaatca aagagtctga taaagaccaa ggaattgaaa cctcaaacat tccaatggtt
     1861 gtttggaaaa attcaaaaca tccagaagtt gctaaagcat tcttagaagc actttataat
     1921 gaagaagact acgttaaatt ccttgattca actccagtag gtatgttgcc aactattaag
     1981 gggattagcg attctgcagc ctataaagaa aatgaaactc gtaagaaatt taaacatgct
     2041 gaagaagtaa ttactgaagc tgttaaaaaa ggtactgcta ttggttatga aaatggacca
     2101 agtgtacaag ctggtatgtt aactaaccaa cacattattg aacaaatgtt ccaagatatc
     2161 attacaaatg gaacagatcc tatgaaagca gcaaaagaag cagaaaaaca attaaacgat
     2221 ttatttgagg ctgttcagta gatgtaaaag actagaaaat aggtgggcta gtgagctgaa
     2281 aagctctagc ccaatcttgt aaaagaaggg agaaggagaa tggttaaaga acgtaattta
     2341 actcgctgga tatttgtttt gccagctatg attatcgtag gattactctt tgtttatccg
     2401 tttttctcga gtatttttta tagctttacc aataagcatt tgattatgcc taattataaa
     2461 tttgttggtt tggctaacta taaagctgtg ctgtcagatc ccaacttctt taatgcgttc
     2521 tttaattcaa ttaagtggac cgttttctca ttagttggtc aagttttagt agggtttgta
     2581 ttggctttag ctcttcacag agtacgccac ttcaagaaat tatataggac cttattgatt
     2641 gttccttggg catttcctac catcgttatt gccttctctt ggcagtggat tctaaacggg
     2701 gtttatggct acttacctaa tctaatcgta aaattaggtt taatggaaca tacacctgca
     2761 tttttgacag atagtacatg ggcattccta tgtttggtgt ttatcaacat ttggtttgga
     2821 gcaccaatga ttatggttaa tgtgctttca gctttgcaaa cagtaccaga agaacaattt
     2881 gaggctgcta agatagatgg tgcttcaagt tggcaggtgt tcaagtttat cgtctttcca
     2941 catattaaag tggttgtagg acttctagtt gttttgagaa ctgtatggat ctttaataac
     3001 tttgacatta tctacctcat tactggtggt ggaccagcca atgctacaac gacacttcca
     3061 atttttgctt acaacctggg ttggggaact aaattgttgg gtcgtgcttc agcagttaca
     3121 gtattgctct ttatcttctt ggtggcgatt tgctttatct actttgctat catcagtaag
     3181 tgggaaaagg agggtagaaa ataatgaaga agaaatccag tatttattta gatattctct
     3241 cacatgtact tttagttggt gcgaccatcg ttgcaatttt cccattggta tggattatca
     3301 tatcttctgt caaagggaaa ggggaattaa ctcagtatcc aacacgattt tggcctgaac
     3361 agtttacatt agattatttc actcatgtta tcaacgattt gcacttcatt gataacattc
     3421 gaaacagttt aatcattgcc ttggctacaa ccctcattgc gattattatt tctgctatgg
     3481 cagcctatgg tattgttcga ttctttccta aattgggagc aatcatgtcg agactactcg
     3541 tcattaccta cattttccca ccaattttgt tagcaattcc ctattcaatt gccattgcta
     3601 aagttgggtt aacaaatagt ttatttggct tgatgatggt ttatctatct tttagtgttc
     3661 catatgcagt ttggctctta gttggatttt tccaaacagt tccaattgga attgaagaag
     3721 cggctagaat tgatggtgca aataaatttg ttacgtttta taaggttgtg ctaccgattg
     3781 tagcaccagg tattgtagca acagctattt atacatttat caatgcttgg aatgaattcc
     3841 tgtatgcctt gattttaatt aacaatacag gaaagatgac agtagcagta gcccttcgtt
     3901 cacttaatgg ttcagaaata ctagactggg gagatatgat ggcagcgtct gttattgtag
     3961 ttcttccatc aattattttc ttctctatca tccaaaataa gattgcaagt ggattatcag
     4021 aaggatctgt gaagtagacg aaagaaggaa aaaaatgaat aaaagaggtc tttattcaaa
     4081 actaggaatt tctgttgtag gcattagtct tttaatggga gtccccactt tgattcatgc
     4141 gaatgaatta aactatggtc aactgtccat atctcctatt tttcaaggag gttcatatca
     4201 actgaacaat aagagtatag atatcagctc tttgttatta gataaattgt ctggagagag
     4261 tcagacagta gtaatgaaat ttaaagcaga taaaccaaac tctcttcaag ctttgtttgg
     4321 cctatctaat agtaaagcag gctttaaaaa taattacttt tcaattttca tgagagattc
     4381 tggtgagata ggtgtagaaa taagagacgc ccaaaaggga ataaattatt tattttctag
     4441 accagcttca ttatggggaa agcataaagg acaggcagtt gaaaatacac tagta
//
LOCUS       DAVLYR010000131         4420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLYR010000131 DAVLYR010000000
VERSION     DAVLYR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.131"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(300..2558)
                     /gene="glgP"
                     /locus_tag="VYO63_001684"
     CDS             complement(300..2558)
                     /gene="glgP"
                     /locus_tag="VYO63_001684"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1672565.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2584..4101)
                     /gene="malQ"
                     /locus_tag="VYO63_001685"
     CDS             complement(2584..4101)
                     /gene="malQ"
                     /locus_tag="VYO63_001685"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1672566.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
BASE COUNT         1276 a          907 c          909 g         1328 t
ORIGIN      
        1 gtgtgaggta atcaactacg aaaaaatata aggctgagga cagtaaaaac cagcatttaa
       61 agtcttcact gtatctagga tttccctatt tttagacaag attgacccaa atctacagca
      121 tttaaaaaag cgaatttcaa tcgaaactcg cttttttaca aaatgtatta gtataaattt
      181 gtatcttata ctcaatgaaa accaaagagt aaactaggaa gctagccgca ggttgctcaa
      241 agcaccgctt tgaggttgca gataaagctg acgtggtttg aagagatttt cgaagagtat
      301 tagttcaagt gccagatgtc ttcgttatac tgagcgattg tacggtcaga tgagaagaat
      361 cctgctttag aaatgttaac gatgacttta tccaaccatg cgtcacggtc ttcgtagtca
      421 gcaagcattt gctctttgac tttgatgtag tcttccaagt caagaagagt catgaaccag
      481 tctttgttga tcaattcatt gtaaagacgt tccaagcgct ctttgtttcc agctgcaaga
      541 actgcatcac taacgatgaa gtcaaccaat ggtttgatag cttcacgagc gtagaattcg
      601 cttgatttgt aagctgcttt tgcgtaaagg tcgataacag tttctgaatc ttcaccgaag
      661 atgtagatgt tttcttctcc aaccaactca gcgatttcca cgttagcacc gtccatagta
      721 ccaagtgtca aagctccgtt caacatgaat ttcatgttac cagtacctga agcttcttta
      781 gaagcaagtg agatttgttc tgagatatca catgctggga taaggaaact tgctgcagta
      841 acgttgtagt tttcaaccat aactacttgc aagtgtggag ctactgctgg atcgttagca
      901 ataacttctg acatgcaaag gattaaatgg ataatgtctt gagcgattgt gtaggctgga
      961 gctgctttac caccaaagaa gattgtgatt ggacgagcag ggatgttacc agctttgatg
     1021 tcaaggtatt tgtggatcac gtacaaagcg ttcatttgtt ggcgtttgta ctcgtgaaga
     1081 cgtttgattt ggatatcaaa gatagaattt ggattgattt ccacaccttg gtgttctttc
     1141 aagtgacgag ccaatttacg tttgttgtga gccttgatgc tttccaattt ttctttgaca
     1201 gctgctttgt cttcataaga caaaagtttt tcaagctcat ctgcttcatg gtgccaacca
     1261 tctccaagaa tctcatccaa gtagtgagac aatcttgggt tagcatgcat aagccaacga
     1321 cggaaagtga taccgtttgt tttgttgttg aacttttctg ggtaaaggtc gtagaaggct
     1381 ttcaactcag aatttttcaa gatttcagta tgaagtgctg caaccccgtt aacactgtat
     1441 ccgtagtgga tatccatgtg agccatgtga acacgtccgc tctcatcgat gatttgaaca
     1501 gctggatctt tgtactctgc cttcacacga cggtccaatt cttcgatgat tggtaccaag
     1561 tgaggaacca cttcttgcaa gaattcaaga ggccattttt caagcgcctc agcaaggatt
     1621 gtgtggttag tgtaggcagt catgctacga acaattgaga ttgcttcgtc aagctcgata
     1681 ccacgtgcag tcaaaagacg aatcaattca ggaatcacca ttgatgggtg agtatcgttg
     1741 atttggacaa ctgcgtagtc agcaaggtca tgcaagttgc ttcctttttc gattgcttcg
     1801 tcgatgatca attgcgcacc gtttgaaacc atgaagtatt gttggaagat acggagcaat
     1861 tcaccttgac ggtcactatc atctgggtaa aggaagagag ttaagttgcg agcgatatct
     1921 gtcttgtcaa agttgatacc atctttaata atagaagaat caactgaatc caagtcaaac
     1981 aaacgcaagc ggtttttagt cgctgtttca taaccagtaa catcaatatc gtaaagagtt
     2041 gatgtcaaag taaagtctgc aaatggtact tggtagctac ggcttgagcg aaccaaccag
     2101 ttttgctctg tcaaccatgc atttggaatt gtttcttgtt ggttgttttt aagaacttgt
     2161 tggaaaagac caaagtggta gttaagacca acaccgtcac cattcaaacc aagagtagca
     2221 attgagtcga taaagcaggc agccaaacgt cccaaaccac cattaccaag agatggttcc
     2281 aattcaactt cttcaacttc gatcaagtct ttacctgcag ctgcaagttc ttttttaaca
     2341 tcgtcgtaaa gaccaaggtt aatcaagttg tttgacaaga gtttaccaat caagaactca
     2401 gctgagatgt agtaaacttt tttcttacca gtgttgactg gtttttggct gcttgcaagc
     2461 ttgctgtagt taagaagagc aaggtaaagc tcttcattgc tacattctgc aatggtttta
     2521 ttgtaacgat tttgtacaaa ttcttgtagt gataacatgt ttagttgtct cctgattatt
     2581 gtcttatttc tttaaatcta ccaaattttc attaattcgg cgataaattg ttgtcaagtc
     2641 aagcaaacct tcctcgacag ctggtgtcaa ttgatcttca gtcatacgcc aagaccagtt
     2701 tccaccaagg gtagatggga agttcatacg agctgcctca tccaattcta gtaaatcttg
     2761 catagttgca attgccataa agctaactga tgaaaatact gtacgaagca tagcgtgtac
     2821 cactgtttcg tattctttac ggttcgtgta acgagccatg tactcacgag tcgcatcatc
     2881 aatctcatta cggtaccaac caagaaccgt attgttatcg tgtgttcctg tgtacataac
     2941 tgagttagca ggtgccaagt gtgggctatc aatgctttcg tcttctgggt tgaaggcaaa
     3001 ttgaagaatc ttcattcctg ggaagccagt acgttcacgc aattcgatca cttcatctgt
     3061 catgaagcca aggtcttctg cgatgatgtt tagctcacca agttcttcct taacggctgc
     3121 aaaaagcttg tagcctggac ctttcaccca ctcaccaggt gctgctgtat cggaaccagc
     3181 agggatttcc cagtaagatt cgaagccacg gaagtggtcg atacgaacga tatcgtagat
     3241 tttgaagctt tcacgcaagc gttcaatcca ccatttgtag ccgtctttgt ccattgcttc
     3301 ccagtcatag attggattac cccaaagctg accagttaca gaaaactcat ctggtgggca
     3361 tcctgcgata caagtagcct taccattgac atctgttttg aagagatgtg gatttgccca
     3421 catatcactt gaatcttccg ctacgtagat tggcatgtcc ccaacgattt cgatgtggtt
     3481 gtcgttagcg taagctttca atttcaacca ttgttggaag aagaagtatt gagtcacacg
     3541 gtggtaaacc aacttgtctg ccaattgctc acgatagctt tcaagtgctg aagctttacg
     3601 agcacgagca tctgcatctg gccattcagt ccaagcaaga ttgtcaaaat actctttgat
     3661 agccatatac tcagcaaaga gctcaagcca tgattggttg tcttgagcaa atttctcaaa
     3721 atctttaaca tctccgactt caaagaaacg tttcaccgct ttttctaaaa gaggacgacg
     3781 tgcatagtag attttagcat agtcaacttc agacgcatcg ctaccaaagt caactccttc
     3841 aaggtcactt gcttccaaca aaccttgctc caccaagata tctaaatcga taaaatgagt
     3901 gtttcctgcg aaggctgaga aagattggta aggagaatcc ccgtaactag ttgctcctaa
     3961 tggaaggatt tgccagtaac gttgttttgt acggaccaag aaatcaacga agtcgtaagc
     4021 actttgacca aatgatccga ttccgtaagc tcctggaaga gaagagatgt gcatcaacac
     4081 accactttga cgttttttca taatagcacc tcgtgtgtta aaataatgga acgttgcgta
     4141 ttttgcagac gcaaacgttt gcgttactta taagtatact ccttttcaag cataattgca
     4201 agcgttttta aaaaactttt ttgattattt ttaaggagac taactcattt tagcaaagat
     4261 aacttaaaac tatctaatcc taaagtttga ctctcacgag acagtctaca aagtacaaaa
     4321 cctccttagg gagcttgaat ttatatgcaa caaagcacaa acgtctatag aaaatagata
     4381 gggattagaa acagggaggt agcccctcct gatttccctc
//
LOCUS       DAVLYR010000132         4403 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLYR010000132 DAVLYR010000000
VERSION     DAVLYR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.132"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(243..794)
                     /locus_tag="VYO63_001686"
     CDS             complement(243..794)
                     /locus_tag="VYO63_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672567.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSTENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1193..2017)
                     /gene="nadE"
                     /locus_tag="VYO63_001687"
     CDS             complement(1193..2017)
                     /gene="nadE"
                     /locus_tag="VYO63_001687"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1672568.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2014..3474)
                     /locus_tag="VYO63_001688"
     CDS             complement(2014..3474)
                     /locus_tag="VYO63_001688"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1672569.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIYNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFPEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3590..4078)
                     /locus_tag="VYO63_001689"
     CDS             complement(3590..4078)
                     /locus_tag="VYO63_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672570.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4068..4277)
                     /locus_tag="VYO63_001690"
     CDS             complement(4068..4277)
                     /locus_tag="VYO63_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1672571.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1246 a         1047 c          827 g         1283 t
ORIGIN      
        1 ctaggattac actataaaaa gtacattacc acctgttcgg ctggagaatc tagtcgatta
       61 gctccctctt agttacaagc ttaaagcgat tttttaacta atactaaacc agattaaaga
      121 aggaactaga acatctgtct tgtactaatc aatatgctat tttaaaattg caaataaact
      181 tggaatgcta attattatat ttacaataaa tagttgactt cgatagaaac agaaaataga
      241 attcaacatt cttccctact tataccataa taaataagat ccgcaagata gccttttctc
      301 tctacaccat taacaatggt ttgtagatag gacattccag ccttttccat gacacgacct
      361 gaagctgggt tgagactggc ataacgtgct ctgacctttt gaaaacctgc ttgagtaaaa
      421 caaaagtcca agacagcttt caaggcctct gtcatcatac catgtcccca gtaagccttg
      481 cctagcacat agccgatttc acagcttaaa tcagcctcgt ctatcttaac gatgctgata
      541 tctcctatca cttgctctgg gttttctttt agacaaatgg cccatttgta atagttgaga
      601 ttagtatagg aagcaaccca attgcgaatc gagtttcgag tgatttcgac atcaggatgg
      661 ggatcccagg taacataggt cagattctca gtggatgaag cccaattttg aaacatggct
      721 tctgcatcac tctccacaaa tcttcttaaa atcaaacgtt ctgtctgtaa tatttgcgta
      781 ccgatagatt tcatgatgct acctcacttc cttatttcta tactcgtagt aagaaggctc
      841 aactcagtaa cagctagaaa acgagtttgt cttctaattt cttgacgcag ttgctgcctg
      901 gtttgaaatg cgctctagca agctttttca aacctagtca tcgttgcagg ggtaagacga
      961 tgaaatcgaa ctcttcgagt tactgaatct gcctcatttt cttatttcta aactcggggt
     1021 tcaagatccc ccagaccttc tctcactttc ttatttcaag atgagaggct gaaaacctcc
     1081 actggaggtt tttcttgctt tttaatttca gggcaagaag ctaaaaaggt cccccggacc
     1141 ttcttcgctt tctcatttct aggctcaggg taaaaacctc cagtggaggt ttttactccc
     1201 aaaagtcatc aaatacagtg atgggtaagt ggcgtttgtg ttggcctttg tgccaccagt
     1261 tttcaatggt cgcttgagct tctgggctga ttgttttgcc ttctaggtag tcgtcaatct
     1321 ctgcgtaggt gactccaagt gcgacttcgt cagctaggcc tggtttatct tcttctaggt
     1381 ctgccgttgg gattttttca taaagggctg gctctgcgcc aagtttctgc aagagctgtt
     1441 ttccttggcg tttattgagg cggtaaagag ggagaatatc cgcaccgccg tcaccaaact
     1501 tggtaaagaa acctgtgata ttttccgcgg cgtggtctgt tccaatgacc gctccgctat
     1561 gggaaccagc aagggcatac tgagcaatca tacggcaacg tgccttgata ttccccttgt
     1621 tgaagtctga aacaggacta cctgtcgctt caactgcagc tgtcatggca tcagctgatt
     1681 ccttgatatt cacaaccaag ctgacatctg gctggatgaa ggctagggct ttttgagcat
     1741 ctgcttcatc agcttgcact ccgtatggca ggcggacagc gataaatttg tagctatcgt
     1801 ctcccgtttc agctcgcagt tcttccatag ctaattgcgc caaacgtcct gccaaggttg
     1861 agtcttgtcc cccagaaatc cctagtacaa aggtttttag gaagggatgt tttttcagat
     1921 atctttttaa gaaatcaata gaacgacgga tttcttcctg ggcatcaatc actggtttga
     1981 cacccagctc ttggataatc gtttcttgca aactcattct tcttctcctt caccaagggc
     2041 ttccttgcgc atcttatcaa tcaagtccat cttatcttgc catacatcac gcgccaaatc
     2101 cactggatag tgctgcggat tgagcacacg cttatactca tcccacaact tgtcaaattc
     2161 cttacgggca taatcctgaa tgtcagtcaa actaggcaag ttgtaaacta atattccttc
     2221 tttgaagata tccaccaaga gaggaacggc atcaaaatta cgaaccgtct tcttgatgta
     2281 tgtataggtc ggatggaaca tcttgatttc tgtcatgtcg ctaatatcca caccatcata
     2341 agtgatgtag tcaccttctg acttgccttt ttcacgactg gtaatgcgcc acacctgctt
     2401 cttacctggc gtcgacactt tttccgcatt attagacagc ttaatcgtat tgcgcatctg
     2461 gccgttttca tcttcgattg caacaatctt gtaaaccgcc ccaagagccg gctggtcata
     2521 ggctgtaatc agcttggtac ccacacccca gacatcaatc ttggcctttt gcatcttgag
     2581 gttaaggatg gtattttcat ctagatcatt agaagcataa atcttagcct ctggaaatcc
     2641 agcctcgtcc agttgctgac ggactttctt agaaatgtag gcaatatccc cagagtcaat
     2701 ccgcacaccc ataaagttaa tctgatcacc cagctcacgc gccacctgaa tggcagctgg
     2761 tacaccgatg cgaagggtat cataggtatc cacaagaaag acacaatttt tgtgggtcgc
     2821 agcgtaagcc ttgaaagcct catagtcatt gccataaacc tgtaccaagg catgggcatg
     2881 ggttcccaaa acaggaatgt caaagagctt acccgcacgc acgttgctgg ttccattggc
     2941 gccaccaatc accgctgcgc gtgttcccca gatggccgca tccatttctt gagcccgacg
     3001 tgtcccaaac tccatcaagg gttcatcttc gataaccgaa cgaatacgag ctgccttcgt
     3061 cgccaccaag gtctggtagt tgacgatgtt caaaagagcc gtttcgacca actgacattg
     3121 ggctagaggt ccttccacct gcacaatcgg ttcattagca aaaaccaaat ccccttcttg
     3181 ggcagaacga acggtcaact ccaacttgaa attgcgaagg taatccaaga acgccccatg
     3241 ataaccaagc gactccaaat aggctatatc actatctgaa aaacgcaagt cttcaagata
     3301 gttcacaatt ctttccaaac ctgcaaaaac cgcatagccg ttcttaaaag gctgttggcg
     3361 gaaatacacc tcaaagaccg ccttcttatt gtaaatccct tgatcaaagt aaacctgcat
     3421 catgttgatc tggtacaagt ccgtgtgcaa tgtcaaacta tcatctggat acatactttt
     3481 cctacttcct tagctagaaa cccatgaaaa ttttcaagaa ctttcatgta ttccaataaa
     3541 ttagtactat tatatcacat tttagctgga ttgagaaaag agtaacaagc tattctccac
     3601 tctccaattc atccatatct tgttcaaatt ttttctgagc ccattcgcca tagctcttaa
     3661 gaccaagatt gccaataaag acccacggaa ggtaaatgac ataagtaatg acccaagcag
     3721 acaggtattt aaaattcaaa ggattgtgct gataaatttc tatgttgaat tgataattct
     3781 gcaacatcaa aagagccgta atagccaagg ttaggaaaaa acaacccaaa atcgtaaaat
     3841 gaaaacgact atagtaggtc actcccagat aacgggcacg attgaaaaag taaaatgtcc
     3901 ctatgatgat aacgattagc agcatattag aattaaaaag gcttggtgct aatactgaaa
     3961 tgatataaga taggagcgac aaagcaatgc agatatagaa actttcagag cccgctttat
     4021 tgaacagttg ttcttctctt tcgtctagta attgataata ataaaatcta tttttcatct
     4081 tcttcctccc aaaatagttg gtctagggtt ttccctaaac atctgcaaat agactggcag
     4141 agcgagagac tgggattgta ttttcccgcc tctatcaaac caatagtctg gcgtgtcacc
     4201 ccgacagcct ctgccagttg accttgtgtt aaatcacgct ctacccgagc taattttaat
     4261 tttaaatttt tagccacctt cgtcctcctt atagttttaa tactcatcta ctcttaaaaa
     4321 atccaaaacc aacacaagct atcagctatt tattataacc ccattaattt gcactccagt
     4381 attatcttca gccgaaaatc agt
//
LOCUS       DAVLYR010000133         4387 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLYR010000133 DAVLYR010000000
VERSION     DAVLYR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.133"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(140..1342)
                     /gene="rpsA"
                     /locus_tag="VYO63_001691"
     CDS             complement(140..1342)
                     /gene="rpsA"
                     /locus_tag="VYO63_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1672572.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRVARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1504..1575)
                     /locus_tag="VYO63_001692"
     tRNA            complement(1504..1575)
                     /locus_tag="VYO63_001692"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1541..1543),aa:Gln,seq:ttg)
     gene            complement(1585..1665)
                     /locus_tag="VYO63_001693"
     tRNA            complement(1585..1665)
                     /locus_tag="VYO63_001693"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1629..1631),aa:Tyr,seq:gta)
     gene            complement(1715..1945)
                     /locus_tag="VYO63_001694"
     CDS             complement(1715..1945)
                     /locus_tag="VYO63_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1672573.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2113..2757)
                     /gene="pcp"
                     /locus_tag="VYO63_001695"
     CDS             complement(2113..2757)
                     /gene="pcp"
                     /locus_tag="VYO63_001695"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1672574.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2772..3695)
                     /locus_tag="VYO63_001696"
     CDS             complement(2772..3695)
                     /locus_tag="VYO63_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1672575.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3692..4381)
                     /locus_tag="VYO63_001697"
     CDS             complement(3692..4381)
                     /locus_tag="VYO63_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1672576.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
BASE COUNT         1386 a          987 c          676 g         1338 t
ORIGIN      
        1 atcgctctga atatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagcttttt
       61 ttcaacccac tacagttgac aaagagccag aaaaatcctt tgtttagtaa acaaaggatt
      121 ttgtgaattt tcaatttgat taaagttcga tatcaccaaa caaatcagcc attgaaaatc
      181 ctgtttgtgt ttctggaagt tcgaaatcac gcttttcttg acgtcttgga cgacgtggac
      241 gagcaacacg tttttcttct ttttgtcctt cttcttgggc tggacgttct tcaagagctt
      301 taatagaaag tgacacgcgt tctgcatctg cgttaacttc aagaacttta acttgaactt
      361 cttgaccaac tttaagagct tcttttggat tttcaatccg tttgtgtgaa atttgtgata
      421 cgtgaacaag tccatcgata cctggcaata cttcaacaaa tgcaccgaag tcagtcaaac
      481 gtttaactgt tccttctact acatcacctt tagccaattt ttgctcaacg ccatcccatg
      541 gtcctggtac tgttgcttta agtgaaagtg atacacgtcc ttcttcttcg ttaagatcaa
      601 ggattttcac ttcaatttct tcaccaacag ttacaactga ttttggtgat acattacgtt
      661 catgtgacaa ttcagtcaag tgaaccaatc cgtcaacacc accaagatcg acgaaagcgc
      721 cgaagcttgt gatacgagca actttaccag ttacaacatc accaacagcc aatttaccga
      781 atacttcagc gcgagctgct gcagtagctg cttcaacaac ttcacgacgt gaaaggatga
      841 agcggttttc tttagcgtta acttctttga ttttagtatc aaattcttga cctacaaaac
      901 gctcagcgtt acgtacgaaa cgagtatcca acattgaagc tgggataaat ccacgaacac
      961 cttcaaattc tactgaaagt ccacctttaa cggcacgcgt tcctttaaca gtaacaactt
     1021 cttcttcgcg accaacaagt ttgtcccatg ctttgcgagc ttcaaggcgt tttttagata
     1081 caaggtatgt aactgtatca gtatctttac caactacttg acgaagtaca agaacatcca
     1141 atacttctcc tactttaaca aagtcattga tatctgcatc acgatcgttt gtcaattcgc
     1201 gaagagtcaa gacaccttca acaccagttc cagagattgc aacgttagct tgagtcgcat
     1261 caactgtcaa tacttcagca ctaacaacat caccagtctc aacttgacta acgctattta
     1321 gcaaatcttc aaattcattc atctaaaaaa tcctccaaca atcaagtttt tcttaaactt
     1381 gacatactta taattttttt cctaagcacc cgcaaggaca tgttcatatc attcacgtct
     1441 ttcaattata aaaataaaat accctaagat attttgcttt ttatcttgat tattacctaa
     1501 gaactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
     1561 cttggctata ccccattaat gaaatggaga gagagggatt cgaacccccg aacccgaagg
     1621 agcggattta cagtccgccg cgtttagcct cttcgctatc tctcctacaa tcaacatgga
     1681 ctattatatc atgaaaattt caaaaaaaca agacttattt tgctaaatta taattttcaa
     1741 tcaaaatttc cacagctttt tccaattttt tataaaaact atcgacattc ccattcttta
     1801 taatggcaaa ttgtccgtct aattcggcaa tttctccgat aactttttta ccgatagtca
     1861 atgtataacc ttcaaaactg tctttaccaa cattaacttt ggcatccgct acttgaattt
     1921 cgattttttt atctttcttg ctcatttcat acctctcttc actttttcta ttttacaaga
     1981 aaatcctaaa actagcaaga aaaaactcta tatcaggctt ctctgatata gagttttatg
     2041 taaacaacct caatcgattt agcatagtta atagttagta aacaagcata attgactatc
     2101 ctttttaggt tatcagtgag tctcaccgcc tactaactgc aaatccttat ctttgtagtc
     2161 tacaatagct tcaatagcta cctctatgcc tcttacaata tcacatagac tcattcctgc
     2221 agtattcggc ttatttacta cttgttccat catatatgga atatgcatga aacctgctct
     2281 catattaggg aattttttat ccactaaata aagagcttgg tacatcaaat gattgcaaac
     2341 aaaggttcct gcactattgg atacaactgc cggaagtccc tgttttttga tagcttgtac
     2401 catcgctttg ataggtaaac tactaaaata ggccgatgct ccatcaatac gaatcggtgt
     2461 atcaattggt tgattgcctt cgttatcagg tatgcgagca tcatcttgat taatagccac
     2521 tcgttcaggt gttaagccgg tcctgccgcc tgcttgtcca atacaaagta cagcatctgg
     2581 ttgatatcgt aatatttctg cctctaaaac ttctgacgac ttataaaaaa ccgttggaat
     2641 ttctacccag cgaacttcag ccccattaat ctcagatggt aataatttta cagcctccaa
     2701 agctggatta atcttttcac ctccaaaagg attaaaacct gtaaccaata ttttcatttt
     2761 attttccttt actaaaatgc gagaaagtac attaagaata tgtgaataac aatcattact
     2821 agagcaacac ctgcttgagc ctttataacg ccattctgat ctttcatatc catcaatgct
     2881 gctggtagag cgttaaaatt agcagccatt ggggtcaata aggtcccaca ataacctgct
     2941 gtcatggcaa gagcaccagc cacaattgga ttagctccca gagcaaatac gaagggaact
     3001 ccaacacctg ctgtaataac ggtgaatgct gcaaaagcat ttcccataat cattgtgaat
     3061 agaaccattc caagaacata ggccaaaact cctataaagc gactatctga aggaacaata
     3121 ccgctaatca gatgagagat aacatcacca acacctgcta cagtaaaaat agcccccaaa
     3181 gcccctaata attgaggaac aatcccactt gttgaaactt gctgagtcat tcgattattt
     3241 tctgataaca gactcttagg gtgactattg gtaatcacaa gaacagaaat tgtagcaaac
     3301 aaggcggcaa ggctaatcga aatcttgcta aattctggaa tcatttgcgc taagaccaac
     3361 gcaagtattg ccatcagcat aactggaata aaaattttat ttttcaacct gttagattca
     3421 atattggctt tcatttcatc taaggatggc aaggttccga tacggacttg cttaaacaat
     3481 gttaacagcg ataataggat tacaataata ccaatactca tatttggcat ataggaacca
     3541 cctataaacg taatagacaa tagagtccaa aatgcagatg tcccaagtcg aactgggttt
     3601 gttttatctt tataactaca ataggctgta tggagaaatt gacaaccaat cacaatatag
     3661 gtcaactcta atagttgctt tgccaactct gtcatttttg ttctcctccc ctagtctttt
     3721 ttgatatcaa ttttttatca aataaataat tgtaaatccc cactacaata agtgttataa
     3781 cagcaacaat aatagatgta gaagcaatcc ctgcataatt gctttcatag cctaactgat
     3841 ctaatgttcc ccctatcaag aggactcccc cagcacctac aaacgtattt tgagcaaaga
     3901 aatttccaaa attttcattc gcagccgcac gcgcttttat tgtctcatct tcaacctctg
     3961 ttaactttct acctaattga gactctgcag ctgcttctcc cataggttga accaaaggtc
     4021 tgacaaactg agggtgtcct cctagacgaa ttgaaaagaa accagctaac tctcgaataa
     4081 agaaataaac tgtatagaag tttccaactg tcagaccttt aatctttcga atcaaatcga
     4141 ttgatcgttg cttgagtcca aaggtttctg acagccccac aagaggcaag gtaaccataa
     4201 aaatcgtgag cactcgctga ttgctaaatt cttttcccaa aatctccaaa aattcaacga
     4261 gagaaacacc tgaaactaaa gctgtaacca aaccagctaa gactactgtt gcaattgtat
     4321 caaattttaa aataaaaccc acaacaatga ttgctattcc tattaatcta atccactcca
     4381 tatcaaa
//
LOCUS       DAVLYR010000134         4359 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLYR010000134 DAVLYR010000000
VERSION     DAVLYR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.134"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            16..1224
                     /gene="sstT"
                     /locus_tag="VYO63_001698"
                     /pseudo
     CDS             16..1224
                     /gene="sstT"
                     /locus_tag="VYO63_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1292..2245
                     /locus_tag="VYO63_001699"
     CDS             1292..2245
                     /locus_tag="VYO63_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1672577.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            2390..2770
                     /locus_tag="VYO63_001700"
     CDS             2390..2770
                     /locus_tag="VYO63_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1672578.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            2869..3090
                     /locus_tag="VYO63_001701"
     CDS             2869..3090
                     /locus_tag="VYO63_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1672579.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3107..3421
                     /gene="trxA"
                     /locus_tag="VYO63_001702"
     CDS             3107..3421
                     /gene="trxA"
                     /locus_tag="VYO63_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1672580.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            3728..4261
                     /gene="queF"
                     /locus_tag="VYO63_001703"
     CDS             3728..4261
                     /gene="queF"
                     /locus_tag="VYO63_001703"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1672581.1"
                     /translation="MKRQSALVVFSGGQDSKTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         1247 a          985 c          847 g         1280 t
ORIGIN      
        1 aaaaaggaga ttattatgaa aagaatcatt catgcttgga ataaggcaag ccttgtcaag
       61 cgtatcttga ttggtatgct ttgtggggaa tcctaggact gacccttcct aatctctcag
      121 ggattgggct actcggggat ttattcgttg gaggtctaaa agctgttgct cctatcctag
      181 tctttgccct cgttgccaat gccctttcca acatcaaaag ggacaagata gcaatatgaa
      241 aactgttgtt ttcttgtata tcctatgata gggactttcg ccgctgctct tgtagctgta
      301 ctagcaagtt tcatcgtccc tattgaaatt accctaaata gtgccaatac tgaaattgca
      361 ccaccagatg ggattgggca ggtcctcagc aacctcttgc tcaaactggt tgacaaccca
      421 gtcaacgccc tgcttactgc taactatatt agaatcttat cttgggcagt catttttgga
      481 atcgctatga gagaagccag taaaaatagt aaagaattgc taaaaactat cgctgacgtg
      541 acttctaaaa ttgtcgaatg gatcatcaat ctggctccat ttggaatcct tggtcttgtt
      601 tttaaaacca tttctgacaa gggagtcgga agccttgcca actacggtat tttattggtt
      661 ctattagtaa cgactatgct ttttgttgcc cctgtggtca accctttgat tgccttcttc
      721 tttatgagac gcaatcctta ccctctagtt tggaactgcc tccgtgtcag cggtgtgaca
      781 gcctttttca ctcgtagttc tgcgactaac attcctgtca acatgaaact ctgccatgac
      841 cttggactca acccagatac ctattctgtt tctatcccac tcggttctac tatcaatatg
      901 gctggagtag cgattaccat taacgttttg acccttgtta cagttaacac tcttggaatt
      961 cctgttgact ttgccacagc ctttgtcctc agtgtggtag cagctatctc agcctgtgat
     1021 gcttcaggta ttgccggagg ttccctcctt cttatcccag ttgcttgtag ccttttcggt
     1081 atttctaacg atattgccat acaaattgtt ggggttggtt ttgtgattgg tgtcatccaa
     1141 gactcatgtg aaacagccct taactcttct acagatgtcc tctttaccgc cgttgccgaa
     1201 tacgcagcaa cccgtaaaaa ataactcatc aaggcaagcc tgctcatgtc ttgtctttta
     1261 cgcttttatt ctaacttatt aggaaattct tatgtctatt agccaacgta cgaccaagct
     1321 catcttagct acctgtcttg cctgcctgct tgcttatttt ctcaatcttt cgtcagcagt
     1381 ttcggctgga attatcgctc tcttgagcct atctgatacg cgtagaagta ctttaaaact
     1441 ggctcgcaat cgtctttttt ctatgcttct agctctggct atcggcgttc tagcttttca
     1501 cttgagcgga tttcatatct ggagtctcgg cctctatctg gccttctacg ttcctttagc
     1561 ctacaagatg ggctgggaaa ttggcatcac accaagcacg gttttggtta gccatctctt
     1621 ggttcaagag tcaacctctc cagacctcct agtcaacgaa ttccttctct ttgctattgg
     1681 tacaggattt gccttgcttg ttaatctcta tatgccttca cgagaagagg aaatccagca
     1741 ctaccacacg ctggtggaag aaaagttaaa agatatcctc cagcgcttca aatactattt
     1801 atccagagga gacggacgca accgagcaca gctggtagca gaattagaca cgcttttgaa
     1861 agaagccctc agactggtct atttggatca ctctgaccac ctctttcacc agaccgacta
     1921 ccatatccac tactttgaga tgagacaacg acaaagtcgt atcctgagaa acatggccca
     1981 acagattaac acttgtcacc ttgccgccag tgaaagcctg atcttagcgc aactcttttc
     2041 aaaaattgca ggtcaactga gccagaccaa tcctgcttct gatttgctag atgaaattga
     2101 acgttatctg gaagtcttcc ggaaccgcag tctgcccaag acaagagaag agtttgaaac
     2161 ccgcgccact cttcttcaac tcctacgtga agccaaaacc ttcatccaag taaaagttga
     2221 tttttaccaa aaatatagac agtaaaaaag aaaaccacta aaaaacagag cttgccagca
     2281 ttcttgaaaa gtagtataat tattgcatgc gcaatcatct tatgacgcaa agactgcccg
     2341 ttaatggact ttcttctata attatgactc taaaaagaaa ggagacacta tgacctattt
     2401 ggaaaaatgg tttgacttca atcggcgtca gaaagaaatt gaaagtctct tggaagagac
     2461 cattgcccaa cagagcgagc aaagtctgac cttgaaagag ttctacctgc tctactatct
     2521 ggacttggct gaagaaaaat ctctacgcca gattgacctg ccagataagc ttcatctgag
     2581 cccgagcgca gtttctagga tggtggctcg cttggaagct aaaaattgcg gtctactcag
     2641 tcgcatgtgt tgccatcaag atagacgctc tagttttatc tgcctgacaa atgatgggca
     2701 aaagacactg gcctcgctac aaaagactgt cgaagaaagc ttggaagcag gtttggattt
     2761 cttgatttaa gatgatttta aaaaaagttg cgtgcgcaat catttttctt gacattctct
     2821 tttataagga gtaaaataaa gttagcatta aacaaaggag ttttaaacat gattgaaatt
     2881 acctatatag atgccagcaa gaacgaaaga actgtgactt tcgagtctta tgaagacttt
     2941 gaacgttcgc aacaagcttg ccttatcggc gtcgcagact actaccctgt ccaaaaatta
     3001 acttacaagg gtcataattt ggactaccat gggacttatg gagatatctt cttctatctc
     3061 atgaaacaag atttaagcca atataactaa aaaaaggaga aatacaatgg caaaagcaat
     3121 tacagatgca acattcgaac aagaaacaaa agacggtttg gtcttagtag acttctgggc
     3181 aacttggtgt ggtccatgtc gtatgcaagg tccaatcttg gacaaattgt ctgaagaact
     3241 ttcagaagat gtcttgaaaa tcgttaaaat ggacgttgat gaaaatccaa acacagctcg
     3301 tgcttttgga atcatgtcta ttccaactct tctcttcaaa aaagacggcc aagttgtcaa
     3361 acaagttgca ggtgttcaca cagcagaaca aatcaaggcc atcattgctg aattgagcta
     3421 atcaaacgag agaccaagta ctttctttgg tctctttttt cttgcccttt gccatttttc
     3481 aaaagatatg ctagactata gtagattgaa actagaatag tacatctcta cttctaaaac
     3541 attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttcttatt tcattttact
     3601 atataaatag aatattacga tatttgggat atgccatcgc taaaaaaaac ctcgatttgg
     3661 tattttttag ctccctaatg ggagcttttt acgtgctctg aacatttccc attttggaag
     3721 gagtactatg aaacgtcaat cagccttggt cgtctttagt ggtggtcaag attcaaaaac
     3781 ctgccttttc tggaccatgc aacactatga aacagtcgaa gctgtcacct ttgcctacgg
     3841 ccaacgtcat cacctcgaaa ttcaaattac tagagaaatc gctaaggaac agggcattcg
     3901 tcaccatatc ctcgatatgt ctctgctggg acaaatcact gctcagccag actttgcgac
     3961 gattcatatt tcctacattc ctgacaagct ctgtgtcgag tcaaaatccc tcaaactata
     4021 tctatttagc taccgaaacc acggagattt ccacgaaaac tgtatcaaca ccatcgggaa
     4081 agacttggtc aacttgctag accctcgcta tttagaagtc tggggaaaat tcactccgcg
     4141 cggtggcatt tcaatcgacc cctactataa ctacggtaag caaggaacta agtatgaggg
     4201 cttggcagaa caacgcctct tccaacacga cctttatcca gagaaaattg acaaccgcta
     4261 aagtcatact caatgaaaat caaagagcaa actaggaagt tagccgcagg ttactcaaag
     4321 cagtgtttga ggttgtagat aaaactgacg aagtcagct
//
LOCUS       DAVLYR010000135         4333 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLYR010000135 DAVLYR010000000
VERSION     DAVLYR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.135"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..2910)
                     /locus_tag="VYO63_001704"
     CDS             complement(<1..2910)
                     /locus_tag="VYO63_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1672582.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTD"
     gene            complement(2920..3375)
                     /locus_tag="VYO63_001705"
     CDS             complement(2920..3375)
                     /locus_tag="VYO63_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin"
                     /protein_id="HEV1672583.1"
                     /translation="MHASTQTGWSAAWLIHFFARLYQGEPAYNQINGLLNNATLGNLF
                     LDHPPFQIDGNLGLVSGICELLVQSHHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSF
                     AWKNGDITFLKLEGGNKDQKVRVRVYGKNTDVQNIELVFNSEKIIELNF"
BASE COUNT         1317 a          902 c          587 g         1527 t
ORIGIN      
        1 atctgttcta ttataaataa ttaacttttc taattcttcg gtttgtgcta aatctatctc
       61 ccaccatgaa ggagaatctc ctttggtatg agtcactgaa tgatgaccat aatctccgtt
      121 tttatttcca tcaactgcta gacgagaaaa accattgtaa tccgttgaac tctgtcgagt
      181 ttctttggtt aatgcaatat tagaaagttt cttcggagtt ctctttgaac catagacttc
      241 aacttccgct aaacttaacg gaatagctgc tgattttaat tctattttga ctagcttagc
      301 ccctacagaa ggtactttta aagataacaa accatcaact actttatcaa aatgtcttct
      361 aaaaacttct tctcctgatg aagatagaaa aataacatca aaattagata aacgctgagg
      421 ttcggcatct gttctattat aaatatcaac tttagaaacc gtaaactctt ctcccagatc
      481 gacctgccac caagcgttag aatctgcctt agtatgagtc accgaatgat gtccgtaatc
      541 tccatcttta ttaccatcaa ctgctaaagc agctacaccc tcataattgg ttgaactttg
      601 agtagctacc ttatttgtag agactacttt atcttctgtt attttatcta tatcctcttc
      661 agattgctta ccatctgatt ctctaaaaac ctctacttct gctaaactca aaggcactcc
      721 actcgttagt aatttaactt taatatacct tgctcctttt tctttgaaat ctagactaac
      781 agattcaccc gacaaattat taacatgttt cttagcaact tcgtttctat tattgtcata
      841 tagaatcaca tcaaaattag atagacgttg agtctcagca tctgtgcgat tataaatttt
      901 aacaagccca actttatcca tttttttcaa atcgacttcc caccaagagg gattatctgc
      961 cctagtgtgt gttaccgaac cagagttaaa attaccattt ctgttaccat ccacagctct
     1021 attagggtct ccattgtaat ctacagaact ttgtgttgtt ggttttgcat atgctatatt
     1081 caaattacca tcattaaatt tatctggtgt tgggaaagag actggacctg tcccctcagt
     1141 atttatagac atctctacca ttccattatg attaaccgta atggtataaa catgggtatt
     1201 ctcatcccaa ttttctgtat aagtataatg atttttatcg ccacttatat ttatctgagg
     1261 tttatgacca gtatgccctt ttaatgtaag tgttgttgtc ctaaagtcat tatctacagg
     1321 attgatggaa taatttttgc ttatccagtt cgctaactct aattcttctt tcttccaact
     1381 ttttgatgca tcaaaatttc ctgataatgc ccacatagct gtcttatctg tcctgtaatt
     1441 attcaataaa atatgtaaat tatttggatt ttctttaaca acagcgtaag tatgtggcgt
     1501 caaatctaac gataacgact ttgtattatt agtatacata ggcaacatta cttgctgatt
     1561 tttattgata ttagcattac tattataaat ataccaggaa ttacctacac gctgagcata
     1621 cccatctcct tcataaagtt ttggataaag cgagtttaaa tagttgactt tactagacaa
     1681 ttcctcacta tttttagtca aaatttttgc attagggaat atagatgaaa tcttttcctt
     1741 atcaattttc tcatgtatta caggaagaat atgatatctc ccattattat ataaaggcat
     1801 tgtttcatca ttcgaataaa gtccttgata aaatccgttt aatgaactaa tcctaccttc
     1861 tccattccaa aatacagctt ttgttctatt tacaacttct tccttacttg gagctggatt
     1921 ttgtatagca tgtctaaaga aaggaataat acctttagta aatgctggag ttggtacatc
     1981 atttgtcata aatgtatacg cggcacattc gaaattataa actgtgcctc ccccagtata
     2041 tacattcatc atttccatag caatcattga ttctggttcc gatgcatagg atctcatatc
     2101 cctagctctt ccagtttcaa atgtgtttgt ataatgtttt tcccaccatt tccatgtatc
     2161 tgttgatgag ccccagttat cacataagcc actcaaccaa aatccactaa cgatagaatc
     2221 tgtacccgca tcatctctta ttggcgtatt tttagttgcc aacaccaaat ttttatgata
     2281 tttttgactc gcttcaaaga atgtcggatc attcataata gtttcccaga accatttttc
     2341 atgatcatgc cagataaaat gcgctccata tttggcacaa acttccaaat atttagcact
     2401 atgcggagct aactggttat tgtaaatcca ataattctca atatttaaaa caccttttaa
     2461 cacactatac ttttggaatt gttcatctaa ccactctgga ggaactgtat tacgctctcc
     2521 agccgacata ataaccaaga aaactggaat gtttagactt tgagcttcct ccaacatctt
     2581 gacataccat tctctcaatt ctttcgtatc tcgtggaata caacttgttg gtttacagac
     2641 ttttgcagga tgaagttcaa tcgctgcata tggttttaca tcttcaggaa tagcttccca
     2701 tgctcccttc aatgtgttcc cccaccataa tccgttacca ttatcattgc catacaacgg
     2761 agaaagcaac aatggcgatt cattattaat cgtcgttctc atttgaacac gattatcagc
     2821 cttaactcct gctaacaatc caaaacataa catcccaata catacaaaca gtattgtgat
     2881 taattttctt tttatttttt ctttattcat gacttatacc taaaaattta actcaataat
     2941 tttttctgaa ttaaatacca attcaatatt ttgtacatca gtatttttgc catatactct
     3001 tactcttact ttttgatctt tgtttcctcc ttccaatttt aggaatgtta tatccccatt
     3061 tttccaagca aacgatacct tatatcctcc tcttactctg aaacctttca cttctccttc
     3121 tgaccaagca gaaggtaaag ctggaattag tgataaccaa ttatgatggc tctgtactaa
     3181 taattcacaa attccactca ccaaacctaa attaccatca atttgaaatg gtggatggtc
     3241 aagaaataaa ttgccaagag tcgcattatt taacaaacca ttaatctggt tataagcagg
     3301 ttcaccttga tatagtctcg caaaaaaatg aatcagccat gcagcactcc aacctgtttg
     3361 tgtactagca tgcaaaccac ttactaacca attattaatc gcttgctccc tatcctgtga
     3421 agataaaaag ttagcgtttg ataatcttct attgatagtg attttagctg cttctgctaa
     3481 ttccggagtt ttatgaatat caatctcatt ataaggataa agcccaaata gaggtgaaat
     3541 gtgtctatga ccaggctcta cttcttcata atcctctagc cattcttgta tttgcccatt
     3601 actacctatt tttgttctag gtaatttctt ttttaactcc ttcacacgac taataaaatc
     3661 cgaattgtct cctaattgtt ttgcaattcc aatgcatgaa tcacaaaaat atcttaaaat
     3721 ttgattatca attgtagatg atagacaagc atttccttca ataccatttt ttaagcgata
     3781 tttattttcc ggtgagacac ttggacctgt catcaagtag ccatccacct caaataaata
     3841 atcttcaaag aaaagaaatg cttcttttat catttcaaaa tgttccgtaa gaatacgctc
     3901 atcttggaaa tataaatagt gttcccaaat atgagtacat aaccatggaa tagttaatac
     3961 ccaaattgca gcccccatgg catgagattg gggagccgta tcgccaaaac catccgtatt
     4021 atgatgtgct gtaaaacctc tagctccata cattttctta gcggttagtc ttcccggttc
     4081 tctcattctt tcgagcatat caaataatgg atattctact tctggtaaat cacatggacc
     4141 taccatccaa taattcattt gagtattaat attaatcgta tatttagaac cccaaattgg
     4201 atttaattca tcacaccata ttccttgaag attggcaggt aaaccattcg gttgactaga
     4261 cgatattaac agatatcttc cataatgaaa taacaagtta gtcaagtagt tactatactt
     4321 tttagtgttt tca
//
LOCUS       DAVLYR010000136         4330 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLYR010000136 DAVLYR010000000
VERSION     DAVLYR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.136"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(221..1195)
                     /gene="pta"
                     /locus_tag="VYO63_001706"
     CDS             complement(221..1195)
                     /gene="pta"
                     /locus_tag="VYO63_001706"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1672584.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1239..2135)
                     /locus_tag="VYO63_001707"
     CDS             complement(1239..2135)
                     /locus_tag="VYO63_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1672585.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHLLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2132..2950)
                     /locus_tag="VYO63_001708"
     CDS             complement(2132..2950)
                     /locus_tag="VYO63_001708"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1672586.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(2934..3605)
                     /locus_tag="VYO63_001709"
     CDS             complement(2934..3605)
                     /locus_tag="VYO63_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1672587.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            3733..4302
                     /locus_tag="VYO63_001710"
     CDS             3733..4302
                     /locus_tag="VYO63_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1672588.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
BASE COUNT         1329 a          929 c          804 g         1268 t
ORIGIN      
        1 tctcttttca tactcattta aaatcaaaaa gggaatttat cgttttacgg acatgtatat
       61 tacagatttc tataggtcgt gtatagacag gatattatcg cttggaagtt ttcctttttg
      121 acaccaatca gtaatttagt tctcaggaat taatggcagt gcttttcgta catagtttca
      181 taactacagt atagcatagt atatcgcttt atagttttca ctattgatga actgcttgag
      241 ctgctgtgat gagggtcaac ttgtaaacat catctgcatt acatccacga gaaagatcat
      301 taactggctt gtttaaacct tgcaaaacag gtcctacagc cgcaaagcca cctaggcgtt
      361 cagccatctt gtaaccgata tttcctgcct cgatacctgg gaagatgaag acatttgctt
      421 gaccagctac cgtacttcca ggagctttca gagctgcagt ttcaggaaca aaggctgcat
      481 caaattgcaa ctcaccatcg atttcaaggt caggacgcaa gtcgtgagca attttagttg
      541 cttcaacgac cttatcaacg ctttcaccaa accctgaacc tttagtagaa tagctcaaca
      601 tggcaatttt aggttcgatg ccaaacatct tagctgtgat tgctgagttg atggcaattt
      661 cagccaaggc ttctgcatct ggattgatgt taatggcaca gtctccaaat aggtaacgtt
      721 ccgtaccacg aaccatgagg aaggctcctg aagtacgagt tacatttgga cgagttttga
      781 tgatttgtag agctggacga actgttgaag ctgttgagtg aatcgctcct gacaccattc
      841 catcaaccaa gcccaagtaa accaacatca caccaaagta gttgacatct tcaaccaaaa
      901 ccttgcgtac atcttcttca gtcattttgc ccttgcgacg ctccaccaag gcagaaacca
      961 tttcttcaaa ttgaggataa tgttgagggt cgatgacctc ataaccatcc atgattcctt
     1021 caatttcaag ataaatttta attttttcag gatttccaag caaaacagga atcacttctg
     1081 tttcttttac taagcgtttt gttgcttgaa gaatacgagg ctcttcccct tcagggagaa
     1141 cgatacgagc atttttacca acaaggttgg ctttgagact ttcaaaaact tccatgagtt
     1201 ttctccttta agataataat tatactctga gacagttttt atagagtatt agttgataac
     1261 tgggtaataa ggttactgaa atcatccggc aagggacttt ctaactgcaa gtcttgctct
     1321 aaaaatggat gataaaagga taggtaatgg caatgcagag cctgacgttg aataccatct
     1381 tccagactac caccatacaa atcatctccc agcaaaggaa aaccgatatg agaaaaatgg
     1441 actcggattt gatgggttcg accagtgtgc aggtgaatat agaccaagtg aatatttcca
     1501 taagaagcta caatcttgta tgaagtatgg gcatactttc cgcctttagc cactcgtctg
     1561 gtaataatgg aatcttcatc acgcgcaatc ggagcaataa tttccccttc tggctccaaa
     1621 tgtccatctc ccttaaccaa agcaaagtag cgtttctcga tagatttctt ctgcaactgc
     1681 ttgtctaatc gtgcatgggc ataaccgtgc ttggcaaaga gcatcaagcc agaagtgtcc
     1741 ctatctagtc tggtaacaat gtgaacctgc tgattttcat aattttgctt gacatagtaa
     1801 cccttgataa aattggcaat ggtattagag tgattgacac taggaataga agccactcca
     1861 tagggtttat tcaagactag aaagtgatca tcctcataga gaatatctaa tagatgctca
     1921 atagcctcca aggtttcaaa gccttcctca gcgggaatgt caatggtaac gtagtctcca
     1981 acgtccaata gatacgttgc attttgcggt tgattattga ccagaatagc tccacctcga
     2041 aacttaatct tggccagcaa tcccttagaa acctcgtgct tttttaaaaa ggtcttaacc
     2101 ttgacatgtt catctgcgat aaattcaaac ctcattcatc cacctcaccg ataaaggcat
     2161 ccttaacacg gttccaaaaa ctggtatggc taggagtcgc gacaaagtga atcttatgat
     2221 ggtcgatttg atactcaata cgctcaatat tacggaaaga ataaacgcta ttgtcaaccg
     2281 aaatagtatg ataatcgttt cttgttggaa taagttcaat cttatccttc ttaggcacaa
     2341 taatggaaga gcccagtgtt cgatagacac gattattaag gctggcaatc tccgttaatt
     2401 gcaaagcttc aatggtaggg tgtaaaacag caccgccaag agacttgtta taggcagtac
     2461 taccagtcgg tgtcgaaact gttagcccgt ctccacgaaa acgttcaaag ggaacaccat
     2521 ttattacaat atctgccacc atggttcgat cagacctgcg gatgctggct tcgttgagtg
     2581 ctctgaaaat cttaacttca ccattttcga gaaagacctt cacattcaga acagggtaag
     2641 aaacccttgc cccagtatct agctgcaaat tagtcactag cttgtccaac tcaaaatcac
     2701 gataatctgt atagaagccc aaatgtccag tatgaagacc gataaagcgg accttgtcaa
     2761 gctgattttc gtacttatga aaggccgaca agagcatacc atccccgcca atggaaatga
     2821 caatatccgg attggtatca ttgagtataa actgatttct cttcaaacga tctcgcaatt
     2881 catacaaaac cctttgactc tgcggttttc tattggctat cagatcaatt cgtttacctg
     2941 tattcttcat ctgtatcgtc actgtttcct acaccgtcat ttaattttct actcaaagga
     3001 tcaaaaagtg cctgggcttc ttggatatca tcacgaattt cacccatttc ttcatccaac
     3061 tgatgggcga ttctagctgt aatttccagt cgcttcttaa tctcatctgg gaaatcccct
     3121 tggtacttgt agttgagaga atgttctatc gttgcccaga aattcatggc caaagtacga
     3181 atttgaattt ctgccaaaat agtcttagct ccattgatgg tatcaaccgt atattctact
     3241 accacatgat aggaacgata gcctgatgct tttctatgag taatgtaatc tcgctcctgt
     3301 atgattcgca tatcctgacg cttgtgcaaa atatccacta cttccttgac gtcatctaca
     3361 aactgaacca tcacacgtaa gccagcaata tcctgcaaat cgtgttccaa ggtcgcataa
     3421 gtaatgccac gacgagccat tttttctttg atgctctcaa ttggcttgac tcgaccggtc
     3481 acaaactcaa ttggagaatg cttattttgc ttacgatatt gcttacgaat accacgaagt
     3541 ttaatcttta actcaccaac agcttgaatg taaggatcta gaaattcttc ccattctaag
     3601 gtcatatatt ctcccttgtt ctcatattat ccgtcaaaaa tatctttgat tttttctcca
     3661 gattcatata caataaatac aatgcttatt ataccataaa tccgctttca acgataaaaa
     3721 aaggtaagaa aaatgaaaca tttagaaatt gaattgaaaa cactattgaa aaaagatgaa
     3781 tacaatcgtc taaaagacca gttcacaggt gtcactcctg ttcttcaaac aaattactac
     3841 atcgacacgc ctgattttga actgcgagaa aagaaagtcg ctatgcgcat tcgaaccttt
     3901 gaagactggg ctgagttgac actcaaagtc ccgcagagtg ttggaaacat ggaatacaac
     3961 caaaaattgc aactaaaaga tgctgagaac tatctgagta aggaagaact tcctcaaggg
     4021 ctagtactgg atgaattggc gaaacatggt atccaaagta agaactggca ggttcttggt
     4081 tgtctaacaa cgcttcgcta tgaaatgaaa acagctattg gtctcatggc actggatcag
     4141 agccgatact ttgatataac agattacgaa ttggagcttg aagtggaaaa tcatgagcaa
     4201 ggcaaacagg atttccgaca atttttagag aaaaatcaga tttcctacca aaaagctcct
     4261 tcaaaattgg ttcgatttgt caaaagtatg aaaaatagct gaaataatct ccattttttg
     4321 gtaaaataga
//
LOCUS       DAVLYR010000137         4284 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLYR010000137 DAVLYR010000000
VERSION     DAVLYR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.137"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1377
                     /gene="galT"
                     /locus_tag="VYO63_001711"
     CDS             <1..1377
                     /gene="galT"
                     /locus_tag="VYO63_001711"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1672589.1"
                     /translation="IGEEGIDRISDENDLKQIKDYLVEIALKNGKIKDLIEEKECLGA
                     ELMNFIVPLPSRLNDIFWSSYDISPQEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTK
                     YGELEITINLSKPEKDPKTIAAEKLVKATNYPKCLLCMENEGYQGRINYPARSNHRII
                     RLKLGDEVWGFQYSPYSYFNEHAIFLNSQHVPMAITSKTFEQLLEIVDILPGYFAGSN
                     SDLPISGGSILSHNHYQGGKHIFPMEKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQS
                     ENKNRLLDLATKILNKWREYSDLEVDIIAMTEDVPHHTVTPIARKVDGRYELDIVLRD
                     NHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPPRLKPELEEVGKYLLGEDNA
                     IADYHLEWADQLKEKYPRINKEEVNSVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMR
                     FVKSVGIN"
     gene            1390..2400
                     /gene="galE"
                     /locus_tag="VYO63_001712"
     CDS             1390..2400
                     /gene="galE"
                     /locus_tag="VYO63_001712"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1672590.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNIFE
                     SVNKIPIPYKIVPRRSGDVATYYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2564..3223
                     /locus_tag="VYO63_001713"
     CDS             2564..3223
                     /locus_tag="VYO63_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1672591.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3248..>4284
                     /locus_tag="VYO63_001714"
     CDS             3248..>4284
                     /locus_tag="VYO63_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1672592.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSKKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWV"
BASE COUNT         1531 a          598 c          874 g         1281 t
ORIGIN      
        1 ataggtgagg agggaattga tcgcatttcc gatgaaaatg acctgaaaca aataaaagat
       61 tacttagtgg aaatcgcctt aaaaaacggt aaaattaaag atttaatcga ggaaaaggaa
      121 tgtttaggag cagagttaat gaatttcata gttcctttac ctagtcgatt aaatgatatt
      181 ttttggagtt catatgatat atcacctcaa gaagcagttg aagagtttta taagttaagt
      241 aaagatagtg attatattaa aacttctgct attgctaaga atattgagtt tcgagcatca
      301 actaaatacg gagagctaga gattacgata aatttgtcaa aacccgaaaa agatccaaaa
      361 acaattgctg ctgaaaaatt agtaaaagca actaattatc ccaaatgttt actatgtatg
      421 gaaaatgaag gatatcaggg gcgaataaat tatccagcac gttcgaatca tagaattatt
      481 cggttgaaac ttggtgacga agtatgggga ttccagtatt ctccctactc atactttaat
      541 gagcatgcga tatttttgaa tagtcaacat gtaccaatgg ctattacctc taaaacattt
      601 gaacaattat tggagattgt tgatattttg ccaggatatt ttgctggttc aaattctgat
      661 cttcctatct caggaggctc cattcttagt cataaccact accagggagg gaaacatatt
      721 ttcccaatgg aaaaagcaaa atttgagagt gaattttgtt ttaaagactt tgaggatgtt
      781 aatgctggta ttgtaaaatg gccaatgtca gtaattaggt tacaaagtga aaataaaaat
      841 cgcttgttag atttagctac taaaattcta aataagtgga gagaatattc agatttagaa
      901 gtagacatca ttgctatgac tgaagatgtc ccacaccata cggtgactcc tattgctcgt
      961 aaagtggacg ggagatacga attagatatt gttctaagag ataatcatac tactgaacaa
     1021 tacccagatg gtgttttcca tcctcatcaa gatgtacaac atattaaaaa ggaaaatatc
     1081 gggttaatcg aagtgatggg acttgcgata ttaccacctc gtttgaaacc agagttagaa
     1141 gaagttggaa aatatctttt aggagaggat aatgcaattg cggattatca tttagaatgg
     1201 gccgaccaat tgaaagaaaa atatcctcgc attaataaag aagaggttaa tagcgtagtt
     1261 caacacgaag caggacaagt gtttgcgaga gttctggaag atgcaggagt ttataagaat
     1321 acaccgtctg ggcatgaagc gtttatgaga tttgttaagt cggttggaat taattaggag
     1381 gaaacccaga tggcaatatt ggtaacaggc ggagctggtt atattggtag ccataccgta
     1441 gtagaattac taaatttagg aaaggaagtt atcattgtcg acaacctttc gaactctagc
     1501 aaattggtgc tagaccgtat tgaagcaatt acaggaatac gtcccgtgtt ttacgaatta
     1561 gatgtttgtg ataaacaagc attgagaaag gtatttgaac aagaatcgat tgatgctgca
     1621 attcattttg caggttataa agctgtcgga gaatccgtgc aaaagcctgt gatgtactac
     1681 aaaaataata ttatgagtac attggcactt gttgaagtga tgtcagaatt taatgttaaa
     1741 aagattgtct tttcttcaag tgcgactgta tatggaatta acaatcagtc acctctaatt
     1801 gagacgatgc aaacaagtgc gacaaatcct tatgggtata cgaaagtgat gcttgagcaa
     1861 attttaaaag atgttcatgt ggcagattca gaatggagta ttgcgttgct tcgttatttc
     1921 aatccaattg gtgctcatga gtctggtttg attggggaag atccctcagg aattcctaac
     1981 aacttgatgc cttatattgc acaagtagcg gtaggtaagc tatcagagct aagtgtgttc
     2041 ggaaatgatt atgatacgct tgatggtact ggtgtgcgtg attatatcca tgtagtagat
     2101 ttagcaatag gacatataaa agctttagaa aaagtatctg aaaaaacaga tgtttatatc
     2161 tataacctag gctcgggaga aggcactagt gtattacaac ttgtaaatat atttgaaagt
     2221 gttaataaga tccctattcc ttataaaata gttccaagac gttcaggaga cgttgcaact
     2281 tattatgcaa atgcagacaa agcatataag gaattaaatt ggaggacaac aaaatcgatt
     2341 gaagacatgt gtagagatac atggaattgg caatcaaaaa atcccaatgg ctataattga
     2401 tgatactgtc tatatgagtg ctgtattttg gaaaaattac ttaacctttt aatgaaagat
     2461 aaattaaaaa aaatgtttga cagcgcttga caaaggaaac ggtttcattg tataatgtaa
     2521 acacaatgac accgatgtca aatataaaaa agaaggtctt tatatgaaaa acttaacaaa
     2581 aattaagttc aaagaaaatg gggaatttaa tcatttccct gggaatacag ttgtagcaaa
     2641 tctttatact aaacaagatt tgatggaagt tgttgatatt attcaatcac gttatagaga
     2701 attaccattt atcgataagt ttactttaac tccaaggaat tctattcaca tgactgtaat
     2761 tgaattgttg tgccatgaaa atcgtgaaac ggagttttgg agcagtaatc ttcctctaga
     2821 tacaccttta caggaaatac atgattactt tgctaaacaa cttgaaattt ttccattgtt
     2881 ggatgaagaa attcatatgc gtataactga aatgggaaaa caaaacatac tagttgaacc
     2941 tgcagatgaa gcttctgcaa agagattaga agaaattcgt acttatgtct cagaaaaagc
     3001 aggtgtttgt ttccctaatc atgatagata tcaattccat atttcaattg ggtatcttcg
     3061 gattcctcta accgaagagg aggaagaaga gtttactaaa gtcagagcag aattaactga
     3121 aattttatta gagaagattc caactattac tgtaaaccgt attgactata ctgtatttga
     3181 agatatgaga caatttgttc catatcacga aaaatttaaa taaaataata aaaaaggaga
     3241 acttatcatg aaaaaaatga aagtttggtc tactgtactt gcaacgggag ttgctcttac
     3301 tacacttgct gcttgctctg gaggttcaaa ttctacgact gcttcttcat ctgaagaaaa
     3361 agctgataaa agtcaagaat tagttatcta ttcgaactca gtctcaaatg gtcgtggtga
     3421 ttggttaact gctaaagcaa aagaagctgg ttttaatata aaaatggttg atatcgctgg
     3481 cgctcaatta gcagaccgtg ttattgctga gaagaataat gcagttgcag atatggtatt
     3541 tggaattggt gctgttgact caaataaaat tagagatcaa aaattactag tacagtacaa
     3601 gcctaaatgg ttagataaaa ttgatcaatc tttatcagat aaagataatt attataatcc
     3661 tgtgattgtt caaccattag ttttaattgg ggcgcctgat gtaaaagaaa tgcctaaaga
     3721 ttggactgaa ttaggtagta agtataaagg taaatattca atttatggtc ttcaaggtgg
     3781 tacaggacgg gcaattctag caagtatctt agttcgttac ctggatgaaa aaggagattt
     3841 aggcgtatca aaaaaaggat gggaagttgc aaaagaatac tttgcaaatg cctatactct
     3901 tcaaaaaggt gaaagctcag ttgttaaagc gttagataaa gaaagcccaa ttcaatacgg
     3961 tatgatctgg ggttctggag cgttaatcgg acaaaaagaa caaaatgttg aattcaaagt
     4021 tatgactcct gagattggtg taccatttgt aactgaacaa actatggttt taagcactag
     4081 taaaaaacaa gcgttagcta aagaatttat tgattggttt ggtcaatcag aaattcaagt
     4141 agaatatagt aaaaaatttg gatctattcc tgcaaataaa gatgccctaa aagagttacc
     4201 tgaagatacg aaaaaatttg ttgatcaagt gaaaccacaa aatattgact gggaagctgt
     4261 tggaaagcat ttggatgaat gggt
//
LOCUS       DAVLYR010000138         4264 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLYR010000138 DAVLYR010000000
VERSION     DAVLYR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.138"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1069
                     /gene="dnaN"
                     /locus_tag="VYO63_001715"
     CDS             <1..1069
                     /gene="dnaN"
                     /locus_tag="VYO63_001715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1672593.1"
                     /translation="SKNAIPILSTIKIDVTNEGITLIGSNGQISIENFISQKNEDAGL
                     LITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPR
                     IQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSH
                     RLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISFY
                     TRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVV
                     SAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPF
                     TLVPADTDEDFMQLITPVRTN"
     gene            1134..1328
                     /locus_tag="VYO63_001716"
     CDS             1134..1328
                     /locus_tag="VYO63_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1672594.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1412..2527
                     /gene="ychF"
                     /locus_tag="VYO63_001717"
     CDS             1412..2527
                     /gene="ychF"
                     /locus_tag="VYO63_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1672595.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2598..3167
                     /locus_tag="VYO63_001718"
     CDS             2598..3167
                     /locus_tag="VYO63_001718"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1672596.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     KADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3168..>4264
                     /locus_tag="VYO63_001719"
     CDS             3168..>4264
                     /locus_tag="VYO63_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1672597.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKF"
BASE COUNT         1459 a          668 c          848 g         1289 t
ORIGIN      
        1 ttctaaaaat gccattccta ttttatcaac aataaaaatt gacgtgacca atgaaggtat
       61 tactttaatt ggttcaaatg gtcaaatttc aattgaaaat tttatttctc aaaaaaatga
      121 agatgctggt ttgttaatta cttctttagg ttcgatcctt cttgaagctt ctttctttat
      181 caatgtagta tctagtttac ctgatgtaac tcttgatttt aaagaaattg aacaaaatca
      241 aattgtttta accagtggca aatcagaaat taccctaaaa ggaaaagata gcgaacaata
      301 tccacgaatc caagaaattt cagcaagcac tcctttaata cttgaaacaa aattactcaa
      361 gaaaattatt aatgaaacag cctttgctgc aagtacacaa gagagtcgtc cgattttaac
      421 aggtgtccac ttcgtattga gtcaacacaa agagttaaaa acagttgcaa cagactctca
      481 tcgcctaagc cagaaaaaat tgactcttga aaaaaatagt gatgattttg atgtcgtaat
      541 tcctagccgt tctctacgcg aattttcagc ggtatttaca gatgatatcg aaactgtaga
      601 gattttcttt gccaataacc aaatcctctt tagaagcgaa aatattagct tctatactcg
      661 tctcctagaa ggaaactatc ctgatacaga tcgcttgatt ccaacagact ttaacactac
      721 tattactttt aatgtggtaa acttacgcca gtcaatggag cgtgcccgtc ttttatcaag
      781 tgcgactcaa aatggtactg tgaaacttga aattaaggat ggggttgtta gcgcccatgt
      841 tcactctcca gaagttggta aagtaaacga agaaatcgat actgatcagg ttactggtga
      901 agatttgacc attagtttca acccaactta cttgattgat tctcttaaag ctttaaatag
      961 cgaaaaggtg accattagct ttatctcagc tgttcgtcca tttactcttg tgccagcaga
     1021 tactgacgaa gacttcatgc agctcattac accagttcgt acaaattaag tgaaagaggt
     1081 tgagcctggc tcgcctcttt tatgatataa tcgaaaaaga aaaggagagt agtatgtatc
     1141 aagttggaaa ttttgttgag atgaaaaaat cacacgcttg tacaatcaag tcgactggta
     1201 aaaaggctaa tcgttgggaa attacacgtg taggagcaga tatcaaaata aaatgcagta
     1261 attgtgagca tgttgtcatg atggggcgat atgattttga gcgaaaaatg aataaaatta
     1321 ttgactgaga acccttagtt agagggttag cactttatcc ctttttgtgt tataatatta
     1381 gggattgaaa tgaaaacgga gaatgagaaa tatggctttg acagcaggta tcgttggttt
     1441 gccaaacgtt ggtaaatcaa cactatttaa tgcaattaca aaagcaggag cagaggcagc
     1501 aaactaccca tttgcgacta ttgatccaaa tgttggaatg gtggaagttc cagatgaacg
     1561 cctacaaaaa ctaactgaaa tgataactcc taaaaagaca gttcccacaa catttgaatt
     1621 tacggatatt gcagggattg taaaaggagc ttcaaaagga gaagggctag ggaataaatt
     1681 cttggccaat attcgtgaag tagatgcgat tgttcacgta gttcgtgctt ttgatgatga
     1741 aaatgtgatg cgcgagcaag gacgtgaaga cgcctttgtg gatccacttg cagatattga
     1801 caccattaac ctagaattga ttcttgcaga cttagaatca gtcaataaac gatatgcgcg
     1861 tgtagaaaag atggcacgta cgcaaaaaga taaagaatca gtagcagaat tcaatgttct
     1921 tcaaaagatt aaaccagtcc tagaagacgg gaaatcagct cgtaccattg aatttacaga
     1981 tgaggaacaa aaggttgtca aaggtctttt ccttttgacg actaaaccag ttctttatgt
     2041 agctaatgtg gacgaggatg tggtttcaga acctgactct atcgactatg tcaaacaaat
     2101 tcgtgaattt gcagcgacag aaaatgctga agtagtcgtt atttctgcgc gtgctgagga
     2161 agaaatttct gaattggatg atgaagataa aaaagagttt cttgaagcca ttggtttgat
     2221 agaatcaggt gtagataagt tgacgcgtgc agcttaccac ttgcttggat tgggaactta
     2281 cttcacagct ggtgaaaaag aagttcgcgc ttggactttc aaacgtggta tgaaggctcc
     2341 tcaagcagct ggtattatcc actcagactt tgaaaaaggc tttattcgtg cagtaaccat
     2401 gtcatatgag gatctagtga aatacgaatc tgaaaaggcc gtaaaagaag ctggacgctt
     2461 gcgtgaagaa ggaaaagaat atatcgttca agatggcgat atcatggaat tccgctttaa
     2521 tgtctaaaaa ttaataaatg gtgtcaatta ggttggaaaa aaattccaac ccttttggct
     2581 tttgaaagga aaaataaatg accaaattac ttgtaggctt gggaaatcca ggggataaat
     2641 attttgaaac aaaacacaat gttggtttta tgttgattga tcaactagcg aagaaacaga
     2701 atgtcacttt tacacacgat aagatattta aagctgacct agcatccttt ttcctaaatg
     2761 gagaaaaaat ttatctggtt aaaccaacga cctttatgaa tgaaagtgga aaagcagttc
     2821 atgctttatt aacttactat ggtttggata ttgacgattt acttatcatt tacgatgatc
     2881 ttgacatgga agttgggaaa attcgtttaa gagcaaaagg ctcagcaggt ggtcataatg
     2941 gtatcaagtc tattattcaa catataggaa ctcaggtctt taaccgtgtt aagattggaa
     3001 ttggaagacc taaaaatggt atgtcagttg ttcatcatgt tttgagtaag tttgacaggg
     3061 atgattatat cggtatttta cagtctgttg acaaagttga cgattctgta aactactatt
     3121 tacaagagaa aaattttgag aaaacaatgc agaggtataa cggataaatg gtgaccttat
     3181 tagatttatt ctcagaaaat gatcagatta aaaaatggca tcaaaattta actgataaga
     3241 aaagacaact aatacttggt ttatcaacat ctactaaggc tcttgcaatt gcaagcagtt
     3301 tagaaaaaga agataggatt gtgttattga cgtcaactta tggagaagca gaaggacttg
     3361 ttagtgatct tatatctatc ttgggtgagg aactcgtcta tccatttttg gtagatgatg
     3421 ctcctatggt ggagtttttg atgtcttcac aggaaaaaat tatttcacgg gttgaagcct
     3481 tgcgtttttt gactgattca tctaagaaag ggattttagt ttgtaatatc gcagcaagtc
     3541 gattgatttt accgtctccc aatgcattca aagatagtat tgtaaaaatc tcagttggtg
     3601 aagaatatga tcaacacgcg tttatccatc agttaaagga aaatggctat cgaaaagtta
     3661 ctcaagtaca aactcagggc gaatttagtc ttcgaggaga tattttagat atttttgaaa
     3721 tatcccagtt agaaccttgt cgaattgagt tttttggtga tgaaattgat ggtatcaggt
     3781 catttgaagt agaaacacaa ttatcgaaag aaaataagac agaactcact atctttccag
     3841 ctagtgatat gcttttgaga gaaaaggatt atcaacgagg acagtcagct ttagaaaaac
     3901 aaatttcaaa aactttatca cctattttga aatcatacct agaagaaatt ctttcaagtt
     3961 ttcaccaaaa acaaagtcat gcagactctc ggaagttttt atctttgtgc tatgataaga
     4021 catggactgt ctttgattat attgaaaaag atactccaat attctttgat gattatcaaa
     4081 aattgatgaa tcagtatgaa gtctttgaaa gagacttagc gcagtacttt acagaagaat
     4141 tacagaatag taaagcattt tctgatatgc agtatttttc tgatattgaa caaatctata
     4201 aaaaacaaag tccagtgacc tttttctcta atcttcaaaa gggtttagga aatctcaaat
     4261 ttga
//
LOCUS       DAVLYR010000139         4259 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLYR010000139 DAVLYR010000000
VERSION     DAVLYR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.139"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..201)
                     /locus_tag="VYO63_001720"
     CDS             complement(<1..201)
                     /locus_tag="VYO63_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672598.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNA"
     gene            complement(476..2215)
                     /locus_tag="VYO63_001721"
                     /pseudo
     CDS             complement(476..2215)
                     /locus_tag="VYO63_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            complement(2378..3262)
                     /locus_tag="VYO63_001722"
     CDS             complement(2378..3262)
                     /locus_tag="VYO63_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1672599.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(3374..4228)
                     /locus_tag="VYO63_001723"
     CDS             complement(3374..4228)
                     /locus_tag="VYO63_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1672600.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
BASE COUNT         1277 a          918 c          677 g         1387 t
ORIGIN      
        1 agcattccat aaaataatca accacattat agcattataa agctgctttg cagtttcatt
       61 ccacttttca ggttgtaaat ttccactccc tgtattgtca tattcttgtc tattatttac
      121 atgagaatca cctacaagcg gattaaaaag aggtagccct ttgtgattaa ttagttctaa
      181 aaaatccctt gccgcaccca tgcaacactg aataacttcc ttcttgccat caattttact
      241 ctcagcgata aatacataat attcatcttc aatagttgct cctgcatcac tatgtttttg
      301 ttgatttgct aacagcttaa tatgtgcttt aggctaaact tcaaaatctc taacaattct
      361 ttgtcttgtt tcatgtcctc tacaattcat ttaagttctc ctttacaagt ttagtaacta
      421 gccttattat actatataag atatgaaatg taccataatc ttcatcaact agtttttaga
      481 aaatcacaaa tccatctgga gagatgacaa gctgattctc caattcttgg caattgcttg
      541 ctagtgctac tgcttctttc tctaaaagat aatcttctgt tgattggttg aatattgctt
      601 tgaggatccg tccttcgtat ttccattcca gagctactag atcagatttg atttcttgaa
      661 ggctatactt gccatgcgaa atgattactg acgcgtattt ccgaatctta atcagcctct
      721 tcataaagtt cagcatatca ttgtcacttg atacacgttc ccaaggcata caacgacgac
      781 aatctggatc tggtcctcca gtcaaggcta gctcggttcc gtaataaatg cacggtgttc
      841 ctttttgtaa aaagagaaag gctaaggctg atttaaccag ttgaacatct tcattggccg
      901 tccacaggat tcgctctgta tcatgtgaat ccaagagatt aaacatgacc tctgaaatct
      961 gctgcttgta atacatagac tctccattga tttcatcgat gaactggtct gtcttcttaa
     1021 ttcctcgtaa gaaatagtcc ttgatactat cagataaagg ataattcatg acggcatgga
     1081 actcatctcc atttagccaa ggctgagatg tatgccagac ttctcctagg atataaagat
     1141 caggattttt agctaaaact gccttacgaa aatccttcca gaactgatgg tcaatctcat
     1201 tagccacatc caaacgccaa gcatcgatat taaactcttc aatccaataa gtcgcaacct
     1261 ttaaaagata attcttgacc tctggattgg ctgtatttag cttaggcata tagtcctcga
     1321 aaccaaaaac atgatagggt aagtctctct tattaactag cttttcagtt gtcactggga
     1381 attgttgaat atggaaccaa tccttataag cagactgttc gccatttttg acgacatttt
     1441 tccattgaag agattgcgaa ccaatatgat taaataccgc atccatcatg actttcatgc
     1501 cacgatgatg cgcttgatcc accagttccc gaaaggtctc cttgtctcca aaataacggt
     1561 caatttcaaa gtaatctgtc gtattgtact tgtgattgct tgtagattca aagatgggac
     1621 aaagatatag tccagtaata cccaagtctt gcaagtaatc catatgatca ataatcccct
     1681 gtaaatcacc accaaagaaa tcatcgctct taggtgtgac agatgaatcc cagtctaaag
     1741 tcccttctgg gtttaataga gcattgccat tggcaaatct ttcaggaaat atctgatacc
     1801 ataccgtatt tgaaacccag tcaggaacct tgcaggcatc aatctcatga agataaggca
     1861 acttaaatcc attcccaatt gcatgaagat tttctagaga attttccaca caccctttat
     1921 cgccatacaa aatattttga ccttctgtat ctctgagctc aaagagatac tggatacgtg
     1981 caaagtcaac tgacacttca acctgccaat ggtcaaataa ggtaccagaa gttatcttga
     2041 ccatttcttt tgtatcctga taaaactcct ccataaagat aaaagggtcc ccatagtgca
     2101 agttgatgct ttcaatgtcc cctttcttag ttcgaattca aatatggagt ttcttatcct
     2161 tataaagata ggcatactcc gactcaggcc tatggtaaat agcactcaat tccatcaatt
     2221 tgtctcctaa agtattcatt caaattttac gcaaacgttt tcaaactcaa taaatccagt
     2281 atactatttt tgtaaactat ttgcaaggaa tgtccgttgt taaaggaact atcttttaaa
     2341 gtatacaaaa agccttaaaa aaggctttct ctatatacta atcctctttc ggttttgaaa
     2401 atacttctac tcgctgagct aggactttga tttctacagg taggttatct gatttatctc
     2461 cgtcaacatc tgactccaat tcactatctg aagagatttt aatattacgc gctttgatat
     2521 actcgatatt atcatttgcg acaacatccc cttttaataa atcaggaatg acggataatt
     2581 tggagaatat agaggcatct ttcagaatca aaatgttggc atagccgtcc ttgttttctt
     2641 caaagatttt cttatcagcg aagtaatttg tcaaaaggac caaaacatgg ctagcttcac
     2701 caacataatt tccattttct gtctcaacct taatgttaaa gacctgatct gtcatgacag
     2761 acttcatggt atttacagca taggttaaaa taccgaattt tgttttgtcc tctatttcaa
     2821 cattgtgaat cgcctcaggc agagaaccga tactaaagat ataaccaaaa tagttgtcat
     2881 ttgctttacc gatatcaatc ttattggtta aatcaaaatc cagttcgtca attgcgccat
     2941 cgatgtcttg attgatttcc aaaagttttg taatgaggtt acccgtaccg cctgggataa
     3001 tccctaactt aggaatgtag tctctctcat caatacctga aatgacttca ttgacagttc
     3061 catctccacc aaacacaacc actgcatcat actgctcacg agaagcttct tcagcaaaat
     3121 gtgttgcatc cagcgctttt tcggtaattt tggtttcaac atattcaaag tattcttttg
     3181 ctttattctc cagcttttct ttgtaatcca aagccttctc gccaccagaa gtagggttga
     3241 taattaccat tgcttttttc attgatttta tccttaattt taaacagaaa tgtttacatt
     3301 tcgtcgtatg caagtaaatg taatcctatt atacaatgaa aatacagaaa agagaaatct
     3361 gacgtactgg agattaatac gcttttattc tattttccca tcgcctaact acatccttta
     3421 agggttcatc caagtaagaa taggccttat ccttgatcca atcaggaata ccgtaagctg
     3481 cctctgctat gctacaagtg attgctgcga gagtatcact gtcgccacca agtgagatgg
     3541 catttcttat cgcatcttcg aagtctctac tttcaagaaa ggcgataatg gcttgaggga
     3601 cagtttcctg acatgtttcg ttaaaacgat agttaggacg gatttcatct aaagtttgag
     3661 atagattgta atcgtattct ttttctatat aatccttaat tcgtctttta cactctgtag
     3721 gattgtcgtt aattgattgc tcgtactctc tacaataacc accaaagtaa aaacgacata
     3781 gaaagatagc atcagctgta gccatagcgc ctttgacacc ttctggatga ttatgagtta
     3841 cctctgcaga aagactcgta agtcctctag atgatggcca cataccagtt ttcgcataaa
     3901 aaccacagtc catgatccaa gcacatggag aaatacgcat agctgatcca ttcccaaagc
     3961 tattataagg ctcacggtta tcgctgttta gccatgcatt aaaccgagca ccgtaatcag
     4021 cattcggata cattctgcca tatttcttca tcgcgtcaat gaaatcgtct ttttgtccac
     4081 cattcataat tgcttctgca acagcacagg tcataaccgt gtcatctgta aaaaagcagt
     4141 ccttccgaaa taaaggaaag tcctttgttt tgatattgtt ccattcgtaa acagaaccga
     4201 caatatctcc aataattgct ccaagcatca gattcctcct tgttcatttt gatgctttt
//
LOCUS       DAVLYR010000140         4146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLYR010000140 DAVLYR010000000
VERSION     DAVLYR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.140"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            31..1206
                     /gene="mutY"
                     /locus_tag="VYO63_001724"
     CDS             31..1206
                     /gene="mutY"
                     /locus_tag="VYO63_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1672601.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1261..1965
                     /locus_tag="VYO63_001725"
     CDS             1261..1965
                     /locus_tag="VYO63_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672602.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            1958..3307
                     /gene="vicK"
                     /locus_tag="VYO63_001726"
     CDS             1958..3307
                     /gene="vicK"
                     /locus_tag="VYO63_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1672603.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3309..4118
                     /locus_tag="VYO63_001727"
     CDS             3309..4118
                     /locus_tag="VYO63_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1672604.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
BASE COUNT         1233 a          744 c          969 g         1200 t
ORIGIN      
        1 aggtgaaacc ttctgtgtta tactagagat atgttagatt tgaaagaata cggtatcgtc
       61 atgtggccgg aggagaaagt catttctttc cgtgagaaac ttctcgcttg gtatgatgaa
      121 aacaaaagag atttgccttg gaggagaagt aaaaatcctt atcacatctg ggtatctgaa
      181 atcatgcttc agcagaccag ggtggataca gttatccctt actacgaaag attcctggac
      241 tggtttccaa ctgtcgaaag tctggcaact gcgcctgagg agagtttact gaaagcttgg
      301 gagggcttgg gctattattc tcgagttcgc aatatgcagg ctgcagccca gcagattatg
      361 actgactttg gtggccaatt tccaaatacc tatgaaggaa tttccagctt gaaagggatt
      421 ggaccttaca cagcaggagc catttccagt attgctttta acttgcctga gccagctgta
      481 gatggtaatg tcatgcgggt cttggcgcgt ctgtttgaag tcaaccacga tattgggatt
      541 ccaagtaatc gaaaaatttt tcaggcaatg atggagatct tgattaatcc ggatcgtccg
      601 ggtgatttta atcaagcctt gatggactta gggtctgata ttgagtctcc tgtaaatccc
      661 agacctgaag aaagcccagt caaggacttt agtgcggcat atcagaatgg aaccatggac
      721 cgttatccaa ttaaatctcc taagaaaaag ccggttccta tttatcttaa agccttagtt
      781 gtgaaaaatt ctcaaggaca atttttactt gaaaaaaatg aaagcgaaaa gctcttggca
      841 ggtttttggc atttcccctt tatagaagtt gataactttt cgcaagaaga gcagtttgac
      901 ctctttcatc aggttgcgga agaaagtgtg aactttggcc ccagtccaga agagagtttc
      961 cagcaggact atgacctaga tgttgattgg cttgatgttt gttttgatac agtccagcat
     1021 gtttttagtc atcgaaaatg gcatgtacag attgtagcag gtcaggtgag tgacttccat
     1081 gatttttcag atagggaagt tcgttggctt tcaccagaag aattcaagaa ttacccactt
     1141 gccaaacccc aacaaaaaat ctggcaggct tatgcacaag ccaacttaga cagtagccaa
     1201 gactagctat tgtgtcttct gactattttt tggtataata gtagagaaaa aggtgaacat
     1261 atgaaaaaaa tactaattgt agatgatgag aaaccaatct cggatattat caagtttaat
     1321 atgaccaagg aaggttatga agttgtaact gcttttaatg gtcgtgaagc gctagagcaa
     1381 tttgaagcag agcaaccaga tattattatt ctggatttga tgcttccaga aattgatggt
     1441 ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc ccattcttat gctttcagcc
     1501 aaagatagtg aatttgataa ggttatcggt ttggaacttg gggcagatga ctatgtaacg
     1561 aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag ctcttctgcg tcgttctcaa
     1621 cctatgccag tagatggtca ggaagcagat agtaaacctc aacctatcca aattggggat
     1681 ttagaaattg ttccagacgc ctacgtggct aaaaaatatg gcgaagaact agacttaacc
     1741 catcgtgaat ttgagctttt gtatcattta gcatcgcata caggtcaagt catcacgcgc
     1801 gaacacttgc ttgagactgt ctggggttat gactattttg gtgatgtccg cacagttgat
     1861 gtgactgtac gacgtctgcg tgagaagatt gaagatacgc ccagccgacc agagtatatc
     1921 ttgacgcgcc gtggtgtagg gtattacatg agaaataatg cttgatttac tgaaacaaac
     1981 catttttacc agagatttta tctttatcct gattttgtta ggtttcatcc ttgttgtgac
     2041 cctcttatta ctggaaaata gacgtgataa tattcagttg aagcaaatca atcaaaaggt
     2101 taaagatttg attgcaggag attattccaa ggttcttgat atgcaaggtg ggtctgaaat
     2161 caccaatatt accaataatt tgaatgactt gtcggaggtt attcgtctca ctcaggaaaa
     2221 tctagaacaa gagagtaaga ggctaaatag tattctgttt tatatgacag atggggttct
     2281 tgcgactaac cgtcggggtc agattatcat gattaacgat acagccaaga agcaactggg
     2341 gttggttaag gaagatgttc tgaatagaag cattttggaa ttgctcaaga tagaagaaaa
     2401 ctatgaattg cgtgatttga ttacccaaag tccagaattg ttgctagatt cccaagatat
     2461 caatggcgaa tatttgaacc ttcgagttcg ctttgccttg atacgtcgag agtctggctt
     2521 tatttcaggt ttggtggctg ttttgcatga tacgacggag caggagaagg aagaacgcga
     2581 acgaagactc tttgtttcca atgttagcca tgagttacgg actcctctga ctagcgtaaa
     2641 atcctatctt gaagccttgg atgagggggc tttgtgtgaa actgtagcac cagactttat
     2701 caaggtttct cttgatgaga ccaaccgtat gatgcgcatg gtgacggatc tcctccatct
     2761 ttcacgtatt gataatgcta ccagtcacct agatgtggaa ctgattaact tcactgcttt
     2821 tattaccttt atcctcaatc gttttgacaa gatgaaagga caggaaaagg agaaaaaata
     2881 tgagctggtg agagattatc ccatcaattc tatctggatg gaaattgata cagataagat
     2941 gacgcaggtt gtcgacaata ttttaaataa tgctattaag tattcgccag atgggggtaa
     3001 aatcactgtc agaatgaaga caactgaaga ccagatgatt ttatccattt ctgaccacgg
     3061 tttggggatt cctaagcagg atttaccacg tatctttgac cgtttctatc gtgtggatcg
     3121 tgctagaagt cgtgcacaag gtggtacagg tctaggactg tctatcgcta aagaaattat
     3181 caaacaacat aagggcttta tttgggccaa gagtgaatac ggcaagggtt caacctttac
     3241 cattgtactc ccttatgata aggatgcagt gaaagaagaa gtatgggagg atgaagtaga
     3301 agactagaat gagtgaaata ggctttaaat acagtatttt agcgtcgggt tccagtggaa
     3361 attcttttta tctggaaacc tcaaaaaaga agcttttagt agatgcaggc ttgtctggca
     3421 agaaaattac cagtctgcta gctgaaatta accgtaagcc agaagacctg gatgccatct
     3481 tgattaccca tgagcattca gatcatatcc atggagtagg cgttttggct cgcaagtatg
     3541 gtatggatct ttatgccaat gaaaagacct ggcaagctat ggaaaatagt aaatatcttg
     3601 gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg taaaaccaaa acctttggag
     3661 atatcgacat cgagagtttt ggtgtaagcc atgatgcagt cgcaccgcag ttctatcgct
     3721 ttatgaagga tgataagagt tttgtcctct tgacagatac aggttatgtc agtgaccgta
     3781 tggcgggcat tgtcgaaaat gcggatggtt atcttatcga ggccaaccat gatgtagaga
     3841 ttttgcgatc aggttcttac gcttggcgac tcaaacaacg aatcctatct gaccttggtc
     3901 acctttctaa cgaggacggt gctgaagcta tgattcggac gctaggaaat cgtactaaga
     3961 agatttacct tgggcattta tctaaggaaa acaatatcaa ggaactggct catatgacca
     4021 tggtcaatca gctggctcaa gctgatctgg gagtcggagt agactttaag gtttatgata
     4081 cctcaccaga taccgcaaca ccattgacag agatatagaa agaacgctga gaaggtgttc
     4141 ttttta
//
LOCUS       DAVLYR010000141         4115 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLYR010000141 DAVLYR010000000
VERSION     DAVLYR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.141"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            577..1569
                     /locus_tag="VYO63_001728"
     CDS             577..1569
                     /locus_tag="VYO63_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1672605.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1571..2389
                     /locus_tag="VYO63_001729"
     CDS             1571..2389
                     /locus_tag="VYO63_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1672606.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2391..3176
                     /locus_tag="VYO63_001730"
     CDS             2391..3176
                     /locus_tag="VYO63_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672607.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3274..3492
                     /locus_tag="VYO63_001731"
     CDS             3274..3492
                     /locus_tag="VYO63_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1672608.1"
                     /translation="MKDLPEWFGIPESTQTYIEGTTTLQVWTAYQESDLTGFVSLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3477..3674
                     /locus_tag="VYO63_001732"
     CDS             3477..3674
                     /locus_tag="VYO63_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672609.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1124 a          722 c          895 g         1374 t
ORIGIN      
        1 aggttatttc aagtcgttcc aaggtaacaa gtctagatca gattgaagct gataaaacga
       61 tacaaagaaa atattcaagt gagctaaaaa aatttattgg attttataat gagattattt
      121 gtgaggaaaa tagtttccta catgtacgaa agaggtggtc gagttggttt aggtagtcga
      181 tgcgtgagtt gataattctc agggtatgga cttctttttc atgaatgagg taaaagagca
      241 ggtattgttt agagacaatc attctgagca tattttctgg atagagggag tatccgattt
      301 tatgatcaaa gttaataccg ccctctggtg agaagatgag taggttggta atttaaacta
      361 ttaaacagaa tttttgatta aaagtattat tttatgagag aaatcctaat ttcacaatcc
      421 ataggcaaac tcttgcattt cgttttttat tggactataa taggttggta taaagccttt
      481 tgtagtaata aaatgtagaa ggtgtagaaa gcaaggattt agaatatttg tagttaaaaa
      541 cacaatgttg ctattcctta cgatagggag atagatatgg caatgataga agtggaacat
      601 cttcagaaaa attttgtgaa gactgttaag gaaccgggct tgaagggggc tttgcgctcc
      661 tttattcatc ctgaaaagca gacctttgaa gcggtcaagg atttgacctt tgaggttcca
      721 aaagggcaga ttttaggatt tatcggggca aatggtgctg ggaagtcgac aaccattaaa
      781 atgctgacag gaattttgaa accaacatct ggtttttgtc ggattaacgg caagattccc
      841 caggacaatc ggcaagatta tgtcaaagat attggcgtag tctttggaca acgcacccag
      901 ctatggtggg atttggctct gcaagagacc tacactgtct taaaagagat ttatgatgtg
      961 ccagactcgc tctttcataa gcgtatggac tttttgaatg aagtcttgga tttgaaggac
     1021 tttatcaagg atcccgtgcg gactctttca ctgggacaac ggatgcgggc ggatattgcg
     1081 gcctccttgc tccacaatcc caaggttctt tttttagatg agccgaccat tggtttggac
     1141 gtttcggtta aggataatat tcgtcgggca attactcaga tcaatcaaga ggaagaaact
     1201 accattcttt tgaccactca cgatttgagt gatattgagc aactttgtga tcggattttc
     1261 atgattgaca aggggcaaga gatttttgat ggaacggtga gccaactcaa ggagaccttt
     1321 ggtaagatga agactctctc ttttgaactg ctaccaggtc aaagtcatct cgtctctcac
     1381 tatgaaggtc tgtctgatat gaccattgat agacaaggaa acagcctcaa cattgaattt
     1441 gatagttctc gctaccagtc agctgacatt atcaagcaaa ccctgtctga ttttgaaatc
     1501 cgcgatttga agatggtgga tacggatatt gaggatatta tccgtcgctt ctaccgaaag
     1561 gagctctagg atgatcaaat tgtggagacg ttataaaccc tttatcaatg caggggttca
     1621 ggagttgatt acttaccgag tcaactttat tctctatcgg attggcgatg tcatgggggc
     1681 ttttgtggcc ttttatctct ggaaggctgt ctttgattct tcgcaagagt ctttgattca
     1741 gggcttcagt atggcggata tcaccctcta catcatcatg agttttgtga ccaatcttct
     1801 gactagatcc gattcgtcct ttatgattgg ggaggaggtc aaggatggct ccattatcat
     1861 gcgtttgttg cgaccagtgc attttgcggc ctcctatctt ttcaccgagc ttggttccaa
     1921 gtggttgatt tttatcagcg ttggccttcc atttttaagt gtcattgtct tgatgaaaat
     1981 catatcgggt caaggtattg tagaggtgct aggattaact gtcctttatc tttttagctt
     2041 aacgctcgcc tatctgatta actttttctt taatatttgc tttggatttt cagcctttgt
     2101 gtttaaaaat ctttggggtt ccaacctact taagacttcc atagtggctt ttatgtcggg
     2161 gagtttgatt cccttggcat tctttccaaa ggttgtttca gatattcttt cctttttgcc
     2221 tttttcatcc ttgatttata ctccagttat gatcattgtt ggaaaatacg atgccagtca
     2281 gattcttcag gcactccttt tgcagttctt ctggctctta gtgatggtgg gattgtctca
     2341 gttgatttgg aaacgggtcc agtcctttat caccattcaa ggaggttagt atgaaaaaat
     2401 atcaacgaat gcatctgatt tttatcagac aatacatcaa acaaatcatg gaatataagg
     2461 tggattttgt ggttggtgtc ttgggagtct ttctggctca aggcttgaat ctcttgtttc
     2521 tcaatgtcat ctttcaacat attccattcc tagaaggttg gacctttcaa gagatagctt
     2581 tcatttatgg attttccttg attcccaagg gaatggacca tctctttttt gacaatctct
     2641 gggcactagg gcaacgccta gtccgaaaag gagagtttga caagtatctg actcgtccca
     2701 tcaatcctct ctttcacatc ctagttgaaa cctttcagat tgatgccttg ggtgaactct
     2761 tagtcggtgg tattttattg ggaacaacag tgaccagcat tgtttggact cttccaaaat
     2821 ttctgctttt cctagtttgt attccttttg cgaccttgat ttatacttct cttaaaatcg
     2881 caacagccag tatcgccttt tggactaagc agtcaggcgc catgatttac atcttctata
     2941 tgttcaatga ctttgctaag tatccgattt ctatttacaa ttctcttctt cgttggttga
     3001 ttagctttat cgtgcctttc gcctttacag cctactatcc agctagctat ttcttacagg
     3061 aaaaggatgt gttctttaac gtaggaggtt tgatgttgat ttctctggtt ttctttgtta
     3121 tttcccttaa actttgggat aagggcttag attcctacga aagtgcgggt tcgtaaaagc
     3181 taaagtaaga ctaaaatcaa gaaaggaact tatgatgttt gtaattgaag aagtcaagga
     3241 tgaaaatcaa aaaaggcagt tgtcgctgag attttgaagg atttgccaga atggtttgga
     3301 atcccagaaa gcacacaaac ctatatagaa ggaaccacga cactgcaagt ttggaccgcc
     3361 tatcaggaga gtgatttgac tggatttgta agcttatcct attcgagtga agattgtgca
     3421 gagattgatt gtctcggcgt aaaaaagctt atcaaggtag aaaaattggg agccaattgc
     3481 ttgctacttt agagagtgaa gctcgtaaaa aagttggtta tctgcaggtc aaaacagtgg
     3541 cagaagcttc taataaagat tatgatcgaa caaatgactt ttatcgaggt cttggcttta
     3601 aaaagttaga gatttttcct caactatgga atccgcaaaa tccttgtcag attttgatta
     3661 aaaagcttga ataatattac ttgacatcta ttttttattc tcagagtgct atactgtaag
     3721 tgtaatcgcc gatttagctc agttggtaga gcaaggcact cgtaaagcct aggttatagg
     3781 tagataaacg actgaggagt tgaaaaaata gataggtaga agataaccgt taagccttat
     3841 tcttagcggt tatttatatt gtttaatagc gctgatattt catcaattat gcctgttttc
     3901 gtgtttccgg tagttgttca agtttattgc tactattttt gatggtatga atgtgcttat
     3961 aatgtatccc ggttaacgaa agttttggac ttatactctt agaaaatctc ttcaaaccac
     4021 gtcaacgccg ccttgccgtg cgtatggtta ctgacttcgt cagttctatc cacaacctca
     4081 aaacagtgtt ttgagctgac ttcgtcagtt ctatc
//
LOCUS       DAVLYR010000142         4111 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLYR010000142 DAVLYR010000000
VERSION     DAVLYR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.142"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            109..1809
                     /locus_tag="VYO63_001733"
     CDS             109..1809
                     /locus_tag="VYO63_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1672610.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1802..2278
                     /gene="ilvN"
                     /locus_tag="VYO63_001734"
     CDS             1802..2278
                     /gene="ilvN"
                     /locus_tag="VYO63_001734"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1672611.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2344..3366
                     /gene="ilvC"
                     /locus_tag="VYO63_001735"
     CDS             2344..3366
                     /gene="ilvC"
                     /locus_tag="VYO63_001735"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1672612.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3473..3709
                     /locus_tag="VYO63_001736"
     CDS             3473..3709
                     /locus_tag="VYO63_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672613.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3947..>4111
                     /locus_tag="VYO63_001737"
     CDS             3947..>4111
                     /locus_tag="VYO63_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1672614.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTL"
BASE COUNT         1290 a          706 c          953 g         1162 t
ORIGIN      
        1 taaacgttaa aatagaaaat tcagaaaatt tcttctttta tcttgaaaaa ttttgaaaaa
       61 atggtatgat agtaacaagt tatttttaag aggaaagaaa ggggaacaat ggagaaaatc
      121 agtttagaat ctcctaagac ggggtcggac ctagttttgg aaacacttcg tgatttagga
      181 gttgatacca tctttggtta tcctggtggt gcggttttgc ctttttatga tgcgatatat
      241 aattttaaag gcattcgcca cattctaggg cgccatgagc aaggttgttt gcatgaagct
      301 gaaggttatg ccaaatcaac tggaaagttg ggtgttgccg tcgtcactag tggaccagga
      361 gcaacaaatg ccattacagg gattgcggat gccatgagcg atagcgttcc ccttttggtc
      421 tttacaggtc aggtggcgcg agcagggatt gggaaggatg cctttcagga ggcagacatc
      481 gtgggaatta ccatgccaat cactaagtac aattaccaag ttcgtgagac agctgatatt
      541 ccgcgtatca ttacggaagc tgtccatatc gcaactacag gccgtccagg gccagttgta
      601 attgacctac caaaagatat atctgcttta gaaacagact tcatttattc accagaagtg
      661 aatttaccaa gttatcagcc gactcttgag ccgaatgata tgcaaatcaa gaaaatcttg
      721 aagcaattgt ccaaggctaa aaagccagtc ttgttagctg gtggtggaat tagttatgct
      781 gaggctgcta cggaactaaa tgaatttgca gaacgctatc aaattccagt ggtaaccagt
      841 cttttgggac aaggaacgat tgcaacgagt cacccactct ttcttggaat gggaggcatg
      901 cacgggtcat tcgcagcaaa tattgccatg acggaagcgg actttatgat tagtattggt
      961 tctcgtttcg atgaccgttt gacggggaat cctaagactt tcgctaagaa tgctaaggtt
     1021 gcccacattg atattgaccc agctgagatt ggcaagatta tcagtgcaga cattcctgta
     1081 gttggagatg ctaagaaggc cttgcaaatg ttgctagcag aaccaacagt tcacaacaac
     1141 actgaaaagt ggattgagaa agtcactaaa gacaagaatc gtgttcgttc ttatgacaag
     1201 aaagagcgtg tggttcaacc gcaagcagtt attgaacgaa ttggtgaatt gacgaatgga
     1261 gatgccattg tggtaacaga cgttggtcaa caccaaatgt ggacagctca gtattatccc
     1321 taccaaaatg aacgtcagtt agtgacttca ggtggtttgg gaacaatggg ctttggaatt
     1381 ccagcagcaa tcggtgctaa aattgctaac ccagataagg aagtagtctt gtttgttggg
     1441 gatggtggtt tccaaatgac caaccaggag ttggctattt tgaatattta caaggtgcca
     1501 atcaaggtgg ttatgctgaa caatcattca cttggaatgg ttcgccagtg gcaggaatcc
     1561 ttctatgaag gcagaacatc agagtcggtc tttgataccc ttcctgattt ccaattgatg
     1621 gcgcaggctt atggtattaa aaactataag tttgataatc ctgagacctt ggctcaagac
     1681 cttgaagtca tcactgagga tgttcctatg ctaattgagg tagatatttc tcgtaaggaa
     1741 caggtgttac caatggtacc ggctggtaag agtaatcatg agatgttggg ggtgcagttc
     1801 catgcgtaga atgttaacag caaaactaca aaatcgttca ggagtcctca atcgctttac
     1861 aggtgtccta tctcgtcgtc aggttaatat tgaaagcatc tctgttggag caacagaaga
     1921 tccgaatgta tcgcgtatca ctattattat tgatgttgct tctcatgatg aagtggagca
     1981 aatcatcaaa cagctcaatc gtcagattga tgtgattcgc attcgagata ttacagacaa
     2041 gcctcatttg gagcgcgagg tgattttggt taagatgtca gcgccagctg agaagagagc
     2101 tgagatttta gcgattattc aacctttccg tgcaacagtg gtagacgtag cgccaagctc
     2161 gattaccatt cagatgacgg gaaatgcaga aaagagcgaa gccctattgc gagtcattcg
     2221 cccatacggt attcgcaata ttgctcgaac gggtgcaact ggatttaccc gcgattaaaa
     2281 atccaactta aatttattaa accagcctaa aaggcaataa ataatagaaa agagagaaaa
     2341 gctatgacag ttcaaatgga atatgaaaaa gatgttaaag tagcagcact tgacggtaaa
     2401 aaaatcgccg ttatcggtta tggttcacaa ggacatgcgc atgctcaaaa cttgcgtgat
     2461 tcaggtcgtg acgttattat cggtgtacgt ccaggtaaat cttttgataa agcaaaagaa
     2521 gatggatttg atacttacac agtagcagaa gctactaagt tggctgatgt tatcatgatc
     2581 ttggcgccag acgaaattca acaagaattg tacgaagcag aaatcgctcc aaacttggaa
     2641 gctggaaacg cagttggatt tgcccatggt ttcaacatcc actttgaatt tatcaaagtt
     2701 cctgcggatg tagatgtctt catgtgtgct cctaaaggac caggacactt ggtacgtcgt
     2761 acttacgaag aaggatttgg tgttccagct ctttatgcag tataccaaga tgcaacagga
     2821 aatgctaaaa acattgctat ggactggtgt aaaggtgttg gagcggctcg tgtaggtctt
     2881 cttgaaacaa cttacaaaga agaaactgaa gaagatttgt ttggtgaaca agctgtactt
     2941 tgtggtggtt tgactgccct tatcgaagca ggtttcgaag tcttgacaga agcaggttac
     3001 gctccagaat tggcttactt tgaagttctt cacgaaatga aattgatcgt tgacttgatc
     3061 tacgaaggtg gattcaagaa aatgcgtcaa tctatttcaa acactgctga atacggtgac
     3121 tatgtatcag gtccacgtgt aatcactgaa caagttaaag aaaatatgaa ggctgtcttg
     3181 gcagacatcc aaaatggtaa atttgcaaat gactttgtaa atgactataa agctggacgt
     3241 ccaaaattga ctgcttaccg tgaacaagca gctaaccttg aaattgaaaa agttggtgca
     3301 gaattgcgta aagcaatgcc attcgttggt aaaaacgacg atgatgcatt caaaatctat
     3361 aactaattag aaatatatag cgctggagat gattttatga aaaagattat gagaaaaact
     3421 gcatcgttat tattggttct agttgtataa tgtaattaca ccgtcggtaa tagtgctagc
     3481 agaccaaaat aaagcagatt ggtcgtatga tgaaaatgct gtaattaaca tttatgatga
     3541 tgctaatttt gaagatggta ggttgcatat gaactttgaa caattcttca aattggcaca
     3601 aatatctaga gaagaaggtc ttgaaattca ttctccgttt gagagagctg gtgcgactaa
     3661 atctgctcgt tatatagcga aatggatttt gagaaataaa aaacattaac aaatatagtt
     3721 ggtaaatcat taggacctaa atcagctgtt agattcggag aagctttatc ctatattgaa
     3781 ggtcctcttc gcagaataaa tgagacgata gatggcggtt tatatcaaat agagcaaatt
     3841 attgcatctg gattgaaaga atcgggttta aatgactgga ctgcgaaaac tttagcttca
     3901 gctattcgtg ggatattaga tgtacttatt taggggttga aatcatatga atattaccaa
     3961 tttgttttct atcaagacag gatgtgatga aactgatagg caactgcaaa aactattttt
     4021 tcagttggat ttacaattgg gagaattgac agatcaacta agaaaattag attctaattt
     4081 tgttcctcgt agtcaatttg tagacacgtt g
//
LOCUS       DAVLYR010000143         3978 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLYR010000143 DAVLYR010000000
VERSION     DAVLYR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.143"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            150..470
                     /locus_tag="VYO63_001738"
     CDS             150..470
                     /locus_tag="VYO63_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1672615.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            474..1289
                     /locus_tag="VYO63_001739"
                     /pseudo
     CDS             474..1289
                     /locus_tag="VYO63_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1364..2290
                     /locus_tag="VYO63_001740"
     CDS             1364..2290
                     /locus_tag="VYO63_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1672616.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            2356..2622
                     /locus_tag="VYO63_001741"
     CDS             2356..2622
                     /locus_tag="VYO63_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1672617.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            2615..3313
                     /locus_tag="VYO63_001742"
     CDS             2615..3313
                     /locus_tag="VYO63_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1672618.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            3324..3803
                     /locus_tag="VYO63_001743"
     CDS             3324..3803
                     /locus_tag="VYO63_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1672619.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            3811..>3978
                     /locus_tag="VYO63_001744"
     CDS             3811..>3978
                     /locus_tag="VYO63_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1672620.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVR"
BASE COUNT         1212 a          604 c          954 g         1208 t
ORIGIN      
        1 aaccatacat acggtagggc gacgctgacg tggtttgaag agattttcga agagtattaa
       61 ctattatgct ttacaaacta gtgtagaagt gatatactat aggtgaagtt actagtaggt
      121 attacaaaat agattagaag ggagaagtga tgaaagaaac tcagctatta aaaggtgttc
      181 ttgaaggttg tgtcttggat atgattggtc aaaaagagcg gtatggttat gagttggttc
      241 agactttgcg agaggctgga tttgatacta tcgttccagg aactatttat cctttgttgc
      301 aaaagttaga aaaaaatcaa tggataagag gcgacatgcg cccgtcgcca gatggtccag
      361 atcggaagta tttttcatta atgaaagaag gagaagagcg tgtctcagtc ttttggcaac
      421 aatgggacga tttgagtcaa aaagtagaag ggattaagaa tgggggttaa accatgaaga
      481 aaatgaagta ttacgaagaa acaagcgctt tgctacatga gttttctgag gagaatcaaa
      541 agtattttga ggagttgtgg gaaagtttta atcttgctgg atttctctat gatgaagact
      601 atctcagaga gcagatctat ttgatgatgc tagatttctc agaagcagaa cgagatggca
      661 tgagtgcaga ggattatcta ggtaagaatc ctaaaaaaat aatgaaagag attctcaagg
      721 gagcacctcg cagttctatc aaagagtccc ttttgacgcc aattcttgtc ctggcggtat
      781 tacgttatta tcaactacta agtgattttt ctaaaggtcc tctcttaaca gtcaatttgc
      841 tcacattttt agggcaactt cttatttttc tgattggatt tggacttgtg gccacaattt
      901 tacgaagaag tttagtccaa gattctccta aaatgaaaat tggcacttac attgttgttg
      961 ggactatagt tcttctagtt gttttaggat atgtaggaat ggcaagcttc atacaagaag
     1021 gagcctttta tattccggct ccctgggata gtttgtctgt ctttacgatt tcgctagtta
     1081 tcggtatttg gaattggaaa gaagcggtct ttcgtccatt tgtcagtatg attattgccc
     1141 atcttgtggt gggttctctg ctccgttatt atgagtagat gggaatttca aatgttttcc
     1201 ttacaaaagt tattccttta gctgtcctct ttattggaat ctttgtcttg ttccgtgggt
     1261 ttaagaagat aaaatggagt gaagtatagt caaaaagccg ttgcaaagcg gttttttgtt
     1321 ataatgaagg gaagaatgtt agaaattaaa ggagaaaaat ttgatgagat acataactct
     1381 tggtcaagat gacaaagaat tatcagaaat tgttctcgga atgatgagaa taaaagataa
     1441 gtctgtaaaa gaagttgaag agcttgtaga aacagcactt tctgttggaa tcaatgcctt
     1501 cgacttggct gatatttatg gtcgtggtcg ttgtgaagaa ctgttaggtc ttgtcctaaa
     1561 aaatcgtcca gatttaagag aaaagatgtg gattcagtcc aaatgtggca ttcgcattga
     1621 agaatttacc tattttgatt tttctaagga ctatattata aaatcagtag acggtatttt
     1681 gcaaagattg aagattgatc atctagatag cttgctcctt catcgaccag atgctttgat
     1741 ggaatctgac caagtagcag aagcctttga tctcctttat aaacaaggta aagttcgaga
     1801 ttttggagtt tctaatcaaa atcctatgat gatggagttg cttaaaaaag atgtcaagca
     1861 gccgttagct gttaatcagc tacaattgag tgcggctttt actccaggat ttgaatcagc
     1921 ttttcatgtt aatatggaag atagtcaagc agctatgcga gatggcagca tttttgaata
     1981 ttgcaaatta cacgatgtgg tcattcaagc atggtctgtc ttgcaattcg ggtattttaa
     2041 agggaacttt gttggaaatg agaaatttca agctttaaat caagtacttg atcgtttagc
     2101 tattaaatat ggagtaacct cttcaactat tgccatttct tggatattgc gttatccagc
     2161 aaaaatgcag gcagtcgtag gtacaacaaa tcctaagcac ttgagagaag ttagccgagc
     2221 ggcaaacttt agcttaacaa gaaaagaatg gtatgaaatt tatcttgctg cagggaataa
     2281 tttgccgtaa gtagtagcag gtttgaataa ctcacagtca aaaagccgta taaagtggtt
     2341 ttttgctata attaaatgaa gaatgtagaa ttaaaggaga aaaacatgac atttgaagag
     2401 atcctgcctg ggttaaaggc taagagaaaa tatgtacgaa ctggttgggg aggtgcggaa
     2461 aactatgtcc aactctttga taccatcgag caaaatgggc ttgcacttga aatgacacct
     2521 tatttcctaa tcaacgtttc tggagaagga gaaggttttt ccatgtggag cccgacagtt
     2581 tgtgatgttt tggcaacgga ttgggtagaa gtgcatgact aaacgtgtac tcattacagg
     2641 agtgagttca gggatcggat tggctcaagc tcgcctcttt ttagagaagg gctatcaagt
     2701 ttatggagtt gaccaaggtg aaaagccact cttagagggt gattttcact ttttacagag
     2761 agatttgacc ttggacttgg aacctatttt tgactggtgc cctcaggtgg atgttttgtg
     2821 taatactgcc ggagttttgg atgattacaa accactgttg gaacaaacgg cgcaggacat
     2881 tcaagagatt tttgaaatca actacattat tccagtagag ttgactcggt attatttgac
     2941 acaaatgctg gaaaataaaa agggaatcat catcaatatg tgttccattg cttctagcct
     3001 agcaggtgga ggtggtcacg cctatacttc atccaagcat gccttggctg gcttcaccaa
     3061 gcagttggct ctagactatg ctgaagctgg gattcaggtc tttggtatcg ctccaggagc
     3121 agtcaagaca gctatgaccg ctgcggattt tgagacaggt ggcttagctg actgggtggc
     3181 tagtgaaaca ccaatcaagc gctggattga accagaggaa gtggcagaag tcagtctctt
     3241 tttggccagt ggaaaggcat ctgccatgca gggacaaatt ctgacaatag atggtggctg
     3301 gtctttgaag taggaggagt tggatggaaa tcaaaaatca ctttggggtc tactgtgttt
     3361 gctttgaaaa tggaaagtta ctttgcattg aaaaaacgag aggcccttat caacatcggt
     3421 atgatctacc tggaggcagt cagcaacttg gtgaaggact gacggaaacg ctgactagag
     3481 aagttatgga agagacggga tttactgtta gaagctactc taatcctcga atctacgatg
     3541 ttttcgtcag ggaagagtta aaaaatttta tggttcacca tgtcatagcc ttgtatgatg
     3601 ttgaaatgaa tgagagtgca cctcaagtta cgatttcgga agctgtgtct gatggtgcga
     3661 atgattcact tggatatatt tggatggata ttcaagagat tacagaagaa aatgcatcgc
     3721 cattagtctt gaaggttaag tctgaattat taggattgcc agaactggac aagacttctt
     3781 acatgaattg gaaggtgaag tagagggata atggaactat ttaaaacatg gaagaaaaat
     3841 atggttctct atggtcttaa atctcaaatt ggaactgtct accgaaacaa tgataggaca
     3901 acaagctttt atgatgttgg gaattttcta tacctagcag gggagttgga ttccagattt
     3961 tgggaagatt ttgttaga
//
LOCUS       DAVLYR010000144         3876 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLYR010000144 DAVLYR010000000
VERSION     DAVLYR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.144"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(253..1215)
                     /gene="nrdF"
                     /locus_tag="VYO63_001745"
     CDS             complement(253..1215)
                     /gene="nrdF"
                     /locus_tag="VYO63_001745"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1672621.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1403..3562)
                     /gene="nrdE"
                     /locus_tag="VYO63_001746"
     CDS             complement(1403..3562)
                     /gene="nrdE"
                     /locus_tag="VYO63_001746"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1672622.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(3643..3861)
                     /gene="nrdH"
                     /locus_tag="VYO63_001747"
     CDS             complement(3643..3861)
                     /gene="nrdH"
                     /locus_tag="VYO63_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1672623.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
BASE COUNT         1127 a          716 c          828 g         1205 t
ORIGIN      
        1 aacaaactca tcattcaaat acaaaaacaa caaaatgaaa caaaaacaaa aatatcgaag
       61 tttgttttca aaactttcga tatttttgtt gggttattat tttgttgttt ctagtttact
      121 ttttgatggt taagagtggt ggagaattat actcaatgaa aatcaaagag caaactagga
      181 agctagccgc aggctgtact tgagtacggc aaggcgaagc tgacgtggtt tgaatttgat
      241 tttcgaagag tattagtcca aaccgtagtt gtagtcatca tcttgcatgg cttcaacttc
      301 gccaagaagg taaccatttc cgacttgaga gaagaagtca tggttggaag ttcctgttga
      361 aataccgttc ataacgattg ggttgacatc ttcagctgaa tctgggaaaa gtggatcttg
      421 tcccatgttc atgagagctt tattggcatt gtagcgaagg aaggttttaa cctcttcagt
      481 ccaaccaaca ccgtcataaa gactctctgt gtagccttct tcattttcat aaagagtata
      541 gagtaggtcg tacatccatt ctttgagttt ttcttgctct tcttcaggta attcattgaa
      601 accaagttgg aatttgtaac caatgtaggt tccgtgaaca gactcgtcac gaataatcaa
      661 tttaatgatt tctgcaacgt tggcaagttt gttgttaccg agatagtaga ggggagtgaa
      721 gaaaccagag tagaagagga aggtttcgag gaagacgctg gcaactttct tttcaagtgg
      781 gctgccgttt aggtagattt cgttgacaat ctcagccttc ttttgtaggt aaggattggt
      841 attggtccat tcgaaaattt cttcaatctc agccttagta ttcaaggtag aaaagattga
      901 tgagtaagat ttagcgtgga cagattccat aaattggatg ttattgaaaa cagcttcctc
      961 atgtggtgta cggatgtctg cgcgaagggc ttgaacccca gtttcagatt gcatagtgtc
     1021 aagaagggtt aaaccaccaa aaacttttcc gaccaagtct ttctctttgt tagatagctt
     1081 tctccagtca tccaagtcgt ttgataaggg aatacgtgta tcgagccaaa attgctccgt
     1141 cagtttttcc caagttgatt tgtcgatgac atcttcgatg gcattccagt taatggcttt
     1201 gtagtaagtt tccatttaaa atctctttct gtgtttagta ttgcgaactc acaattattt
     1261 ctactttacc ataattctat aggagtatcg cacaaaaagt cggaagcccg acttttaaaa
     1321 tgttacataa attatgttat gacatagtag atttgatttt atcagtgctg cttagggaaa
     1381 aatagtgttt ctatgctaga aactaaatca cacagctttc acattggttg gcgccgactt
     1441 ctccaccgtc atctgtaaag gtacggacgt agtagataga cttgattccc ttgttaaagg
     1501 catagttacg aaggatggac aagtcacgtg tcgtttgttt attttccctc ttccattcgt
     1561 aaaggccttt tggaatgtca ctacgcatga agagggtgag tgaaagtcct tgatccacgt
     1621 gttcagtcgc agcagcgtaa acatcgatga ctttacgcat atccatatcg taggcagaag
     1681 tgtagtaagg aatggtttct gtagacaagc cagcagcagg gtagtagatt ttaccaattt
     1741 tcttttcctg acgttcttca atacgttgcg taatcgggtg gatagaagca gaaacatcgt
     1801 tgatgtagct gatagaacca tttggcgcta cagcaaggcg attttggtgg taaagaccat
     1861 cctcttgaac cttgtcgcga agttcagtcc aatcagcaac accagggata aaaacatttt
     1921 tgaagagttc tttaacacgg tctgatgttg gaacaaattc accagttaca tacttgtcaa
     1981 agtaacttcc gttagcatag tctgattttt caaagttgtg gaaggtaata ccacgttcac
     2041 gcgcaatatt gtttgactct accaaggtcc agtagttcat aagcataaag tagatgcttg
     2101 taaattcaac agactcaggt gaaccatatt caatgagttg ttgggcaagg tagctgtgca
     2161 gtcccatggc accgagacca aaggtgtggg cttggctatt tccatggtca atcgttggta
     2221 cagctacgat atgtgaacta tctgtaacga aagtaagggc acgaaccata gcacggatag
     2281 aacgaccaaa atcaggtgaa gtcatcatgt taaccacgtt ggttgaaccc aggttacatg
     2341 aaacatctgt tcccatttga aggaattctt gagcatcgtt gatcaagctt ggttcttgaa
     2401 cttgaagaat ctcagaacac aagttactca tgataatctt tccatcgaca ggatttgcac
     2461 ggttagccgt atctatgttg actacataag gatagccaga ctcttgttgc aatttagaga
     2521 tttcagtttc caaatcccgc gccttgattt ttgtcttgcg aatatttgga tttgcgacca
     2581 attcatcgta tttttcagta atgtcgatgt aattgaatgg cacaccgtat tctttttcta
     2641 cagagtaagg gctgaagagg tacatttctt catttttacg agccaattcg tagaatttat
     2701 caggtactac aacaccaagt gatagagtct tgacacgtac tttttcatca gcgttttctt
     2761 tcttagttga aaggaaagcg atgatatctg ggtgaaagac gttgaggtag acaacaccgg
     2821 caccttgacg ttgccccaat tggttggagt aagagaagct gtcttcaaaa agcttcataa
     2881 caggaacgac acctgaagca gctccttcat agcctttgat aggtgcacca gcttcacgaa
     2941 ggttgctgag ggtaattccc acaccaccac caatacgtga aagttgaaga gctgagttga
     3001 tagaacgtcc gatagagttc atatcatccg tcacttggat taggaaacaa gataccaact
     3061 ccccacgacg agcacgtcca gcattcaaga aggaaggagt agcaggttgg tagcgttggt
     3121 ggatgatttc attggcaata tcgattgcaa cagcttcatt cccatcagcg aaataaaggg
     3181 cgttgaagaa gacacggtct tccatatttt caagatagta ttcaccgtca ttagtcttta
     3241 aggcatattg attgtaaaat ttataagctg ccatgaatga cttgaattgg aagttttggt
     3301 ctttgataaa ttgagccaat tcttccaaga actctggacg gtatttcttg ataaaggctg
     3361 tttcgatgta gttgtgttca atgaggtaat tgattttgtc tttgattgaa tcaaaaacca
     3421 tagtgtttgg aactacattt tctttaaaga aagcatccaa ggcttcctta tctttatgaa
     3481 gcatgatttg tccattaaca ggacggttaa tttcgttatt aagacggaag taagtcacgt
     3541 cttcaagatg ttttaatccc ataaaatttc ccttatctaa ttacaaaaga aaggcttcta
     3601 agttagccct aaaagcagtt tcttctggat gatgtactaa gattatgcta attgtttcag
     3661 tttttctggt tggaaacctg aaaagacttc agttggtgtt tggataacag gagctgcgct
     3721 aaaaccgagc tctttaactt gatcgacgta ctcaggttgc tcatcaagat tgatttcacg
     3781 ataagagaca ttattactgt ccaagaaacg cttggtcatt ttacattgga cacaattgtt
     3841 tttagaataa acggttacca ttgtgtaact cctctt
//
LOCUS       DAVLYR010000145         3856 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLYR010000145 DAVLYR010000000
VERSION     DAVLYR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.145"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            85..1530
                     /gene="cps4A"
                     /locus_tag="VYO63_001748"
     CDS             85..1530
                     /gene="cps4A"
                     /locus_tag="VYO63_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1672624.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVITALVLLVALLGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAVYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1532..2263
                     /locus_tag="VYO63_001749"
     CDS             1532..2263
                     /locus_tag="VYO63_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV1672625.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKVKELFVDNPRKIIMDQLI"
     gene            2272..2964
                     /gene="cpsC"
                     /locus_tag="VYO63_001750"
     CDS             2272..2964
                     /gene="cpsC"
                     /locus_tag="VYO63_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1672626.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSMTRIYVVNRNQGEKSGLTNQDLQAGSSLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKDLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRSTLIGFLAGVIGTSVIVLILELLDTRVKRPKDIEDTLHMT
                     LLGIVPNLNKLK"
     gene            2974..3663
                     /gene="cpsD"
                     /locus_tag="VYO63_001751"
     CDS             2974..3663
                     /gene="cpsD"
                     /locus_tag="VYO63_001751"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEV1672627.1"
                     /translation="MPTLEIAQKKLEFVKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PEEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGTVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GFYGNYGKKII"
BASE COUNT         1245 a          609 c          803 g         1199 t
ORIGIN      
        1 aaagatgcag atagtaaaaa aaatgtagac attaccgtaa aaaagtgata taatcgtatg
       61 atgttcaagg tataggtgtt aatcatgagt agacgtttta aaaaatcacg ttcacagaaa
      121 gtgaagcgaa gtgttaatat cgttttgctg actatttatt tattgttagt ttgtttttta
      181 ttgttcttaa tctttaagta caatatcctt gcttttagat atcttaacct agtgataact
      241 gcgttagtcc tactagttgc cttgctaggg ctactcttga ttatctataa aaaagctgaa
      301 aagtttacta tttttctgtt ggtgttctct atccttgtca gctctgtgtc gctctttgca
      361 gtacagcagt ttgttggact gactaatcgt ttaaatgcga cttctaatta ctcagaatat
      421 tcaatcagtg tcgctgtttt agcagatagt gatatcgaaa atgttacgca actgacgagt
      481 gtgacagcac cgactgggac tgataatgaa aatattcaaa aactactagc tgatattaag
      541 tcaagtcaga ataccgattt gacggtcaac cagagttcgt cttacttggc agtttacaag
      601 agtttgattg caggggagac taaggccatt gtcctaaata gtgtctttga aaacatcatc
      661 gagtcagagt atccagacta cgcatcgaag ataaaaaaga tttataccaa gggattcact
      721 aaaaaagtag aagctcctaa gacgtctaag aatcagtctt tcaatatcta tgttagtgga
      781 attgacacct atggtcctat tagttcggtg tcgcgatcag atgtcaatat cctgatgact
      841 gtcaatcgag ataccaagaa aatcctcttg accacaacgc cacgtgatgc ctatgtacca
      901 atcgcagatg gtggaaataa tcaaaaagat aaattaaccc atgcaggcat ttatggagtt
      961 gattcgtcca ttcacacctt agaaaatctc tatggagtgg atatcaatta ctatgtgcga
     1021 ttgaacttca cttctttctt gaaaatgatt gacttattgg gaggggtaga tgttcataat
     1081 gatcaagagt tttcagctct acatgggaag ttccatttcc cagtagggaa tgtccaccta
     1141 gactctgagc aggctctagg ttttgtacgt gaacgctact cactagccga tggagaccgt
     1201 gatcgtggtc gcaaccaaca aaaggtcatt gtagcaatta ttcagaagtt aacttctaca
     1261 gaggttttga aaaactatag tagtattctt caaggattgc aggattctct tcaaacaaat
     1321 atgccgattg agactatgat agatttagtg aatactcagt tggaaagtgg ggggaattat
     1381 aaagtaaatt ctcaagattt aaaagggaca ggtcggatgg atcttccttc ttatgcaatg
     1441 ccagacagta acctctatgt gatggaaata gatgatagta gtttagctgt agttaaagca
     1501 gctatacagg atgtgatgga gggtagatga aatgatagac atccattcgc atatcgtttt
     1561 tgatgtagat gacggtccca agtcaagaga ggaaagcaag gctctcttgg cagaatccta
     1621 caggcagggg gtgcgaacca ttgtctctac ctctcaccgt cgcaagggca tgtttgaaac
     1681 tccggaagag aagatagcag aaaactttct tcaggttcgg gaaattgcaa aagaagtggc
     1741 agatgattta gtcattgctt atggcgcaga gatatactat actctggatg ctctagaaaa
     1801 gctagaaaaa aaagaaattc ctacccttaa tgatagtcgt tatgccttga ttgagtttag
     1861 catgcatact tcctatcgtc agattcatac gggattgagc aatattttga tgttgggaat
     1921 cacgccagta attgctcata ttgaacgtta tgatgcttta gagaataacg aaaaacgtgt
     1981 tcgtgaactg attgatatgg ggtgctatac tcagataaat agttatcatg tttcaaaacc
     2041 taagttcttt ggtgaaaaat ataaattcat gaaaaagaga gctcggtatt ttttggaacg
     2101 tgatttagtt catgtagttg caagtgacat gcacaattta gacagtagac ctccatatat
     2161 gcaacaggca tatgatatca ttgctaagaa atatggagcg aaaaaagtaa aagaactttt
     2221 tgtagataat cccagaaaaa ttataatgga tcaattaatt taggagaaaa tatgaaggaa
     2281 caaaacactt tggaaatcga tgtattgcaa ctattcagag ctttatggaa aagaaagttg
     2341 gtcattttat tagtggcaat tataacttct tcagttgctt ttgcctacag tacttttgtt
     2401 atcaaacctg agtttactag tatgactcgg atttatgtag ttaaccgtaa tcagggagag
     2461 aagtctggtt taaccaatca agacttgcag gcaggatcat ccttggttaa agactatcgt
     2521 gaaattatcc tatcgcagga tgttttggag gaagttgttt ctgatttgaa actagatttg
     2581 acgccaaaag atttggctaa taaaattaaa gtaacagtac cagttgatac ccgtattgtc
     2641 tctgtttcag ttagtgatcg agttcctgaa gaggcaagcc gtatcgctaa ctctttgaga
     2701 gaagtagctg ctcaaaaaat tatcagtatt actcgtgttt ctgatgtgac aacactggag
     2761 gaggcaagac cggcgacatc accgtcttcg ccaaatatta aacgcagtac actaattggt
     2821 tttttggcag gagtgattgg aactagtgtt atagttctta ttcttgaact tttggacact
     2881 cgtgtgaaac gtccgaaaga tatcgaagat acactgcata tgacactttt gggaattgta
     2941 ccaaacttga ataagttgaa ataggagaga ggaatgccga cattagaaat agcacaaaaa
     3001 aaactggagt tcgttaagaa ggcagaagaa tattacaatg ccttgtgtac aaatatacag
     3061 ttgagcggag ataaactaaa agtaatttcc gttacttctg ttaaccctga ggaaggaaaa
     3121 acaactactt ccgtaaatat agcaaggtcg tttgcgcgtg caggctataa aactcttttg
     3181 atcgatggcg atactcgaaa ttcagttatg tcaggatttt ttaaatctcg tgaaaaaatt
     3241 acagggctaa cggaattttt atctgggaca gctgatttat ctcacggttt atgtgataca
     3301 aatattgaaa atttatttgt agttcaatcg ggcactgtat caccaaaccc tacagccttg
     3361 ttacaaagta aaaattttaa tgatatgatt gaaacattgc gtaaatattt tgattatatc
     3421 attgttgata cagcacctat tggaattgtt attgatgcgg caattatcac tcaaaagtgt
     3481 gatgcgtcca tcttggtaac agcaacaggt gaggtgaata aacgtgatgt ccaaaaagcg
     3541 aaacaacaat tagaacaaac agggaaactg ttcctaggag ttgttttgaa taaattagat
     3601 atctcggttg ataagtatgg agtttacggt ttctatggaa attatggtaa aaaaataatt
     3661 taggaaagat tctatggatg aaaaaggatt gaaaattttt ttggcagtat tacagagtat
     3721 tattgtcatt ttattggttt attttcttag ctttgttaga gagacagaac ttgaacgttc
     3781 ttcgatggtt atactatacc ttctccactt ttttgtattc tattttagtt cctatggtaa
     3841 caattttttt aaaaga
//
LOCUS       DAVLYR010000146         3790 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLYR010000146 DAVLYR010000000
VERSION     DAVLYR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.146"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(110..1336)
                     /locus_tag="VYO63_001752"
     CDS             complement(110..1336)
                     /locus_tag="VYO63_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1672628.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLT
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1408..2073)
                     /locus_tag="VYO63_001753"
     CDS             complement(1408..2073)
                     /locus_tag="VYO63_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1672629.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2141..2338)
                     /locus_tag="VYO63_001754"
     CDS             complement(2141..2338)
                     /locus_tag="VYO63_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV1672630.1"
                     /translation="MLNLQFAETMELTEAELETGYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            complement(2624..3487)
                     /locus_tag="VYO63_001755"
     CDS             complement(2624..3487)
                     /locus_tag="VYO63_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1672631.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFLYIPIFE
                     AELESMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFTQSLQVLENPQLTSLFE
                     LRLQQYKKLID"
BASE COUNT         1309 a          733 c          693 g         1055 t
ORIGIN      
        1 agattcagtc tactataaca cattcagaaa agagaaaaag tctgttgatt ttgaccatca
       61 taaaaagact ggcaatccag tctcaaacat atattataga aattctccac taaatacttt
      121 cacgaatatt cagaagcata acaaaggcaa ctagaagaaa tagcaataaa acaaagctaa
      181 ctgccagagt tccaaagcta gtagcaatgg ttaccaaagc tattgtaaat aagctaggta
      241 aaacaaccgt aatggcaccg atagaggatt gaactgctcc cattgactcc tcaggtattt
      301 gtttaaaaac gagttcttgc aatctaggag agagaacacc tgcgaaaaag gcatccaagg
      361 tactaaagat gagaatccag tcaaaacgaa ctgtggcaaa tcctactaga agaagcaact
      421 ggatgacaag tgaggcatag agagctgttt ttatggaaat ggtatgttgc agatagccac
      481 ttacaaggct tccgacaatc agggctgaca gttcaaaagt ggatagcaag gcaagagatt
      541 gacctgtttg taaattcaaa aagggctggt tccttaaaaa tagagtggaa ataggaaccg
      601 taacatttat cactgcttga ctagtagaga taataaacaa aaccaagagc accttattca
      661 tattccatat caattttgat gattggagca aatgctggca aaaggatttt acagagagtc
      721 cttcttgata gctaatcgtt ttttctactt tcaagaggtc agtttttatg aagaggatac
      781 ctaaaaatgc gattaaaaag gtaagagcgt tcagtaagga aataaactgg atggatagaa
      841 tgcctagtaa gactcctcct aggatattac tgattgtttt cactaaacta acagttgact
      901 gtttaaagcc aatagcttct gccagatggt cttgcccaat aattctaatg aaaatcggag
      961 tgagcatggc tcctgaaaag taactcaatg tgtcagacaa gaggttaagc agacaaataa
     1021 atgctactag caacaaggag aaagactgcc ctgaaagtga taaagaaacg atagagtaaa
     1081 gcaaaaattt tgcaaaacta atgactgtgt atttcaagac acgatgatgt tgaaaatctg
     1141 ccaaaactcc cagaaagatt tgtaaaattt ggggaagggt ttctgaaatc gtaatgagta
     1201 aaatcgtcaa aggtgcaaaa gacgcatctg ccacataatt cagaaaggcc agataaaaaa
     1261 tcgtatcccc aagcgttgaa atccactggt tgatagttaa ttgcctaaaa tctctatttt
     1321 gaagaaatac tttcatcaca actccttctt aagttcaaat gggaatcttt ccccaaggat
     1381 agaccgcgat actactaaca accaaaatta cagtaacatc aaaagctgac caatgccatt
     1441 gtagactata tgcagtccaa taggccaata aattgacttt gtcattctaa ataagactgc
     1501 aaatataaga cctccaccca tatagaagac aaagtctgtc aagacccaac cgtgattact
     1561 aatgtgcgag accccaaata aaacagcgga accaagtaca tctagccccc atttctttcc
     1621 tttttccaga gcagtcatca ctaatccacg ataaatcatg tcttcaaaaa tgggacctgc
     1681 aatcacagga taaaaaaaat acatcaaaaa tgccgtagct cctgtaaaag taggagcagc
     1741 atgttgataa gaaatttcat ttcgagtagg tgggaaaaga aaaaaggtga caaaattcca
     1801 agcaacaaaa gcaagcagag ctaggaagga atagaaaaga taggatcctt taaactttct
     1861 actattgatt ttctgccatt tccccgacca aatcatagca ataagagcaa ataaaaccac
     1921 aagaaaattc aacatcatat ccgacagata ataggcaaag tcagatagcc cagcaacaag
     1981 gtcgctgcgt aaaactaaaa cactgaactt ctggtcagca ataactagta gaaaaactat
     2041 aataaagtag cggtgtgaga ttatcttttt cattctattt tctcctatac tatgaaataa
     2101 agacagtccc tacccaagaa aaaggcaagg actctgaata tcaataatgg cgacgattaa
     2161 cagtggaaga atcatggtta aaatatttct tccagaaatt agtgattgac caagacgttc
     2221 ctaaacctcc ccaagcaccg gctgggataa cagcattatt cccaaattcc cctccataac
     2281 ctgtctccaa ctcagcttct gtcaattcca ttgtttctgc aaattgtaaa tttaacatct
     2341 tttacactcc ttcaattatc ttcatttgta aaccacttct gcgacctagg atttgcttca
     2401 aatgttttac aaggacagta taacacggaa attggcttat tttagaaaat cgcatatttg
     2461 atattttttc ttatagaaat ttcagatttg cgattttggt agatttgatt atttccctgg
     2521 tataataaag ttattactaa tgaggaggga agtaatatga agaaacaagt tttaacatta
     2581 ttgacgatcg ttgctgagat tattattatt ttgccatttc taactaatcg ataagctttt
     2641 tgtattgctg caaacgcaat tcaaaaaggg atgttaattg tggattttct aatacctgca
     2701 gagattgggt aaagcgttca atctcttttt gattgcttcc ctttgtttga agaaagacac
     2761 tcatcttctt taaaaattgc cacgatactt tttcaaaaac atcgtacgga cgcaacatgc
     2821 tctccaactc ggcttcgaag attgggatgt ataagaaaag ttttcgctcc atgagttctg
     2881 ataagatatt taagagtcct tgcttcatat acaatcgatt gtgtactaac tctttaaatt
     2941 ctttggattt ttcgagtaag gaggttgata aaaagatcag atcttgattg ctcaagaagg
     3001 gcagagtatt gcaaaagaga tagagttcaa accaggtcca agactcgata gcatagagat
     3061 aggtggtcaa aaactcgcta tcctcctctg ctagtgggta gcttttatct agtgagtgga
     3121 tggcatcttt gattacaata gcattcaaac gacgataagt ctctgccatc tgttcttgct
     3181 cgacttcctc caacagttgc tctaagccag ctatatcttg atgagcaaag cgatccacaa
     3241 cctttctacc aatctgcata tgtggagatt cttgatagtt gttgagcttg tgcccaaact
     3301 catcaaaggt cacattgatc ccttggatgg ctaatatgag cttatcagca gacaacatgg
     3361 actgccctag ctcaaacttg gacaactgag aagctgttag cccgtcacaa gccacatctg
     3421 actgcttgag ctttctcgcc aaacgtaatt ccttgtaaaa ttcccccagt tccatttttt
     3481 caatcatcct accacctcct agcttttgca tattatatca ttatttgtgt tcatttgtca
     3541 aaacattctg acactttggt gattgaaaaa gccagcctta agctgactct aaaaggaccg
     3601 agccttgtac aatacagaac tcaatcctcg ttctaaatag aatgtctaac ttttttgggc
     3661 agtacacgag cttggggaac agccactgga acgatgaggt gtgagctcaa aatatcctcc
     3721 aattatgttt ttcctaatag cataccggaa gagtgaaagg attttataat ggagcggtta
     3781 caaagaacct
//
LOCUS       DAVLYR010000147         3738 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLYR010000147 DAVLYR010000000
VERSION     DAVLYR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.147"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            70..1251
                     /locus_tag="VYO63_001756"
     CDS             70..1251
                     /locus_tag="VYO63_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1672632.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1309..2067
                     /locus_tag="VYO63_001757"
     CDS             1309..2067
                     /locus_tag="VYO63_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1672633.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIIYFSNQEEYSRIINSLK"
     gene            2280..3641
                     /gene="dnaA"
                     /locus_tag="VYO63_001758"
     CDS             2280..3641
                     /gene="dnaA"
                     /locus_tag="VYO63_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1672634.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1326 a          647 c          672 g         1093 t
ORIGIN      
        1 ttttgtgttt aagttataat taagctagtc agtttatact ttcagtaatt caaacatatg
       61 gaggcaaata tgaaacatct aaaaacattt tacaaaaaat ggtttcaatt attagtcgtt
      121 atcgtcatta gcttttttag tggagccttg ggtagttttt caataactca actaactcaa
      181 aaaagtagtg taaacaactc taacaacaat agtactatta cacaaactgc ctataagaac
      241 gaaaattcaa caacacaggc tgttaacaaa gtaaaagatg ctgttgtttc tgttattact
      301 tattcggcaa acagacaaaa tagcgtattt ggcaatgatg atactgacac agattctcag
      361 cgaatctcta gtgaaggatc tggagttatt tataaaaaga atgataaaga agcttacatc
      421 gtcaccaaca atcacgttat aaatggcgcc agcaaagtag atattcgatt gtcagatggg
      481 actaaagtac ctggagaaat tgtcggagct gacactttct ctgatattgc tgtcgtcaaa
      541 atctcttcag aaaaagtgac aacagtagct gagtttggtg attctagtaa gttaactgta
      601 ggagaaactg ctattgccat cggtagcccg ttaggttctg aatatgcaaa tactgtcact
      661 caaggtatcg tatccagtct caatagaaat gtatccttaa gatcggaaga tggacaagct
      721 atttctacaa aagccatcca aactgatact gctattaacc caggtaactc tggcggccca
      781 ctgatcaata ttcaagggca ggttatcgga attacctcaa gtaaaattgc tacaaatgga
      841 ggaacatctg tagaaggtct tggtttcgca attcctgcaa atgatgctat caatattatt
      901 gaacagttag aaaaaaacgg aaaagtgacg cgtccagctt tgggaatcca gatggttaat
      961 ttatctaatg tgagtacaag cgacatcaga agactcaata ttccaagtaa tgttacatct
     1021 ggtgtagttg ttcgttcggt acaaagtaat atgcctgcca atggtcacct tgaaaaatac
     1081 gatgtaatta caaaagtaga tgacaaagag attgcttcat caacagactt acaaagtgct
     1141 ctttacaacc attctatcgg agacaccatt aagataacct actatcgtaa cgggaaagaa
     1201 gaaactacct ctatcaaact taacaagagt tcaggtgatt tagaatctta attgacatct
     1261 atgtaaagaa agctttacat aagagaaaag atgtgttagt gtagaatcat ggaaaaattt
     1321 gaaatgattt ctatcacaga tatacaaaaa aatccctatc aaccccgaaa agaatttgat
     1381 agagaaaagc tagatgaact agcacagtct atcaaagaaa atggggtcat tcaaccgatt
     1441 attgttcgtc aatctcctgt tattggttat gaaatccttg caggagagag acgctatcgg
     1501 gcttcacttt tagctggtct acggtctatc ccagctgttg ttaaacagat ttcagaccaa
     1561 gagatgatgg tccagtccat tattgaaaat ttacagagag aaaatttaaa cccaatagaa
     1621 gaagcacgcg cctatgaatc tctcgtagag aaaggattca cccatgctga aattgcagat
     1681 aagatgggca agtctcgtcc atatatcagc aactccattc gtttactttc cttgccagaa
     1741 cagattctct cagaagtaga aaatggcaaa ctatcacaag cccatgcgcg ttccctagtt
     1801 gggttaaata aggaacaaca agactatttc tttcaacgga ttatagaaga agatatttct
     1861 gtaaggaagt tagaagctct tctgacagag aaaaaacaaa agaaacagca aaaaactaat
     1921 tatttcatac aaaatgaaga aaaacagtta agaaaactac tcggattaga tgtagaaatt
     1981 aaactatcta aaaaagacag tggaaaaatc attatttatt tttcaaatca agaagaatat
     2041 agtagaatta tcaacagcct gaaataaggc tgttctttta tttttttatc tcacaaggtt
     2101 atccactatg tttttcgata aaaagcttaa taaatcaata atttcttctt ttatccccaa
     2161 cctgtggata aagtttagta acattgtgga ttatttttca cagcttgtgg aaaattcttg
     2221 ctatctatgg taaaatatct ctagtattaa acttttaaat agtaaaggag gaaaaaggat
     2281 tgaaagaaaa acaattttgg aatcgtatat tagaatttgc acaagaaaga ctgactcgat
     2341 ccatgtatga tttctatgct attcaagctg aactcatcaa ggtagaggaa aatgttgcca
     2401 ctatatttct acctcgctct gaaatggaaa tggtctggga aaaacaacta aaagatatta
     2461 ttgtagtagc tggttttgaa atttatgacg ctgaaataac tccccactat attttcacca
     2521 aacctcaaga tacgactagc tcacaagttg aagaagctac aaatttaact ctttatgact
     2581 atagtccaaa gttagtatct attccttatt cagatacggg attaaaagaa aagtatacct
     2641 ttgataactt tattcaaggg gatggaaatg tttgggctgt gtcagccgct ttagctgtct
     2701 ctgaagattt ggctctgacc tataaccctc tttttatcta tggaggacca ggccttggta
     2761 agactcactt attaaacgct attggaaatg aaattctaaa aaatattcct aatgcgcgtg
     2821 ttaaatatat ccctgccgaa agctttatta atgactttct tgatcaccta agacttgggg
     2881 aaatggaaaa gtttaaaaag acctatcgta gtcttgatct tttgttaatc gatgatatcc
     2941 agtcactcag cggaaaaaaa gtcgcaactc aggaagaatt tttcaatacc tttaacgccc
     3001 ttcatgacaa gcaaaaacag attgtcctaa cgagtgatcg tagtccaaaa catctagaag
     3061 ggctcgagga gaggcttgtc acgcgtttta gttggggatt gacacaaact atcacacccc
     3121 ctgactttga aacacgtatt gccattttac aaagtaaaac ggaacattta ggctacaatt
     3181 tccaaagtga tactctagaa tacctagctg ggcaatttga ttcaaatgtt cgagatcttg
     3241 agggagccat caacgacatc actttaattg ccagagtaaa aaaaatcaag gatatcacta
     3301 ttgatattgc tgcagaagcc attagagccc gcaaacaaga tgttagccaa atgctcgtca
     3361 tcccaattga taaaatccaa actgaagttg gtaactttta tggtgttagt atcaaagaaa
     3421 tgaagggaag tagacgcctt caaaatattg ttttggcccg tcaagtagcc atgtatttat
     3481 ctagagaact aacagataat agtcttccaa aaattgggaa ggaatttggg ggaaaagatc
     3541 ataccacagt cattcatgcc catgccaaaa taaaatcttt gattgatcaa gacgataatt
     3601 tacgtttaga aattgaatca atcaaaaaga aaatcaaata atttgtggat aacttttagt
     3661 tttttatctt ttttatccac attttttaaa caagctaaaa aacttgatag gacttgttta
     3721 aaggctgttt tccacaga
//
LOCUS       DAVLYR010000148         3711 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLYR010000148 DAVLYR010000000
VERSION     DAVLYR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.148"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..504
                     /locus_tag="VYO63_001759"
     CDS             <1..504
                     /locus_tag="VYO63_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672635.1"
                     /translation="NLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDE
                     PTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAP
                     SELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITL
                     TGGKLNA"
     gene            497..1210
                     /locus_tag="VYO63_001760"
     CDS             497..1210
                     /locus_tag="VYO63_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672636.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(1532..1624)
                     /locus_tag="VYO63_001761"
                     /pseudo
     CDS             complement(1532..1624)
                     /locus_tag="VYO63_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1945..2646
                     /gene="pyrF"
                     /locus_tag="VYO63_001762"
     CDS             1945..2646
                     /gene="pyrF"
                     /locus_tag="VYO63_001762"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1672637.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            2680..3312
                     /locus_tag="VYO63_001763"
     CDS             2680..3312
                     /locus_tag="VYO63_001763"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1672638.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKDDQENWQEG"
BASE COUNT         1157 a          652 c          754 g         1148 t
ORIGIN      
        1 aatttgttaa agcgagtagg attatatgat agttgcaaga tggtagcagg aaaattatcc
       61 actggaatga ggcaacgaat gcttttagca cgagctctta tcaacaaacc cgctgtactc
      121 tttctggatg aaccgacctc aggtctagat cccacaactt ctcgaacaat tcatgagtta
      181 attttagaat tgaaaacagc agggacaacg atttttctaa cgactcatga tatgaatgaa
      241 gcaactcttt tatgtgatta tgttgcctta ttaaataaag ggaaattagt tgagcaagga
      301 gctccttctg aactcattca aagatataat aaagataaaa agattaaggt tacagattat
      361 aatgggaatc agataacttt tgattttaca tcactagaac aggtatctca ggctgatctg
      421 gaaaatattt tttcaattca ttcatgtgag cctactttag aagatatttt tatcacatta
      481 acaggaggaa agctaaatgc ttaaacggtt tctggctttg gtatggttgc gttgtcaaat
      541 tatcctttcc aataagagta ttttattgca agttttagtg ccttttgctt tcacatattt
      601 ttataaatat cttatggaaa cacaggggaa ggtcaacgat caacaggcat tagttctttt
      661 gatgatgtgt ttaccttttt ctttggcttt ggctgttgga agtcctataa ctattatctt
      721 gtctgaagaa aaagaaaagt acaatttaca aactcttctg ttgagtggtg ttaaaggatc
      781 cgaatacatt ttatcaacga tgtttcttcc ttttttgcta acttttgtga ttatgggaac
      841 tactcctctt attttaggag ttacaattgt acatactttt aattatatta caatcgttct
      901 tctaacctct ttatccatca ttttattcta tttattgata ggtttaaccg cgaagagcca
      961 agtagtagct caggttatca gtcttcctgc tatgatttta gttgctttct taccgatgct
     1021 atctggtttg gataagacag ttgcgaagat aacagattat agttttatgg gactgtttac
     1081 taagtttttc acaaaatggg agggattttc atggaatgaa actctaattc ctaatctaac
     1141 actacttatt tggattgttc ttctattaac tttaattacg ataactatta ggaaaaagaa
     1201 aatttcttaa ttgagttatt ttaatgatta taaacacaag tgggaaggaa aaatgaactg
     1261 atctttttga cagcaattct acagaatagt cttattgcta tattttgatt tgagtgtacg
     1321 aaaaaagaaa aataacaata gtgctcatac taattgcaga agttttgggt gataagataa
     1381 ctgataaatt gcaataaaaa atgcaacatt tttaaatctc ctctataagt gcttcaaaac
     1441 ctgtcttgta atccaagtat ttttggggac ggtgattaat aagctagcaa agcaccatta
     1501 aggatttttt cggtaattgt tgccaaatcg gtttaagaaa atactcacga agaagtccat
     1561 tcgcattctc attacttccc ctttgccaag atgaataggc atccgcaaaa taaaacagaa
     1621 ttcccatttg ttcaattaaa gggtaacaag caaactcttt ttctctgtcc taagtgaaag
     1681 tctttaacta ttcttttgga aagagtcttg tgaggtgttc aatagcagtc aacacatacg
     1741 tgaccatgtt tttacttgac aagtgctagt agaaataata gaatagtaaa aaacctttaa
     1801 agcagtccag agaggcagct aaggttagac ggtgaaaggg tggagactac ccatttttcg
     1861 tggaaccttg ctgttggcag gttccttttt tcgtggcttc tgttggccag actctctcac
     1921 tagtaaaggt aaaaggagaa acctatgcga gaacatcgtc caatcattgc tcttgatttt
     1981 cctagttttg aggcggtcaa ggaattttta gctcttttcc cagcagaaga aagcctttat
     2041 ctcaaggtag ggatggagct ttattacgca gcggggcctg agattgtgtc ctacttaaaa
     2101 ggtttgggtc atagtgtctt tttggatctc aaacttcatg acattcctaa tacagtcaag
     2161 tcagcaatga agatcctatc tcagcttggt gtggatatga ccaatgttca tgcggctggt
     2221 ggtgtagaga tgatgaaggc tgcgcgtgaa ggtcttggaa gtcaagccaa attgatcgct
     2281 gtaactcagc tcacatcaac gtcagaagct cagatgcagg agtttcaaaa tatccaaacc
     2341 agtctgcaag agtctgtgat tcactatgcc aagaagacag ctgaagctgg cttggatggt
     2401 gttgtttgct cggctcagga agtacaagtc atcaagcagg ctaccaatcc agattttatc
     2461 tgtctgacac cagggattcg tccagctggt gttgcagttg gagatcaaaa acgagtcatg
     2521 acacctgctg atgcctatca aatcggcagt gactatatcg tagtgggacg tcccattacc
     2581 caagctgagg atcctgttgc agcttatcat gccatcaagg atgaatggac acaggactgg
     2641 aattaaagaa ctagattaga aaaataaaag gagaatacca tgacacttgc taaagatatc
     2701 gctagccacc tcttgaaaat ccaagccgtt tacctcaaac cagaggaacc cttcacttgg
     2761 gcatctggta tcaagtcacc gatttacact gataatcgtg tgacactagc ctatccagaa
     2821 actcgtaccc taattgaaaa tggttttgtg gaagctatca aagaagcctt tcctgaagta
     2881 gaagtgattg caggaactgc aacagcaggg attccacacg gagccattat tgctgataag
     2941 atggacttgc cttttgccta catccgtagc aaaccaaaag accatggagc tggtaatcaa
     3001 atcgaaggtc gcgtagctca aggtcaaaaa atggtagtgg ttgaagacct tatttcaacg
     3061 ggtggttcag ttcttgaagc tgtagcagca gccaagcgag aaggagcaga tgtgcttgga
     3121 gttgtagcga ttttcagcta ccaattgcca aaagcagata agaactttgc agatgctggt
     3181 gttaaacttg tgacgctttc aaactatagc gagcttatcc atctagccca agaagaaggt
     3241 tacatcacgc cagagggcct tgatcttcta aaacgcttta aagacgacca agaaaattgg
     3301 caagaaggtt aggtcagtaa gataaagaga gacgaggcta ccgagcctct tttaccattt
     3361 tatttaaaat atgacaatat tttaaataaa ataattgaca acgctttctt ttattgatat
     3421 aatgaaaatg taatatgtat caaattgatc aatcaactat cttgtttatc acttcttaag
     3481 aggacgatgt catgaaaaga agaaaaatat attccttttt tgcgtagtta caattgcatc
     3541 tagtttgttt ctccctacag ttttagctag acagattgga aattatgatt taacgttgcc
     3601 gcgttggggt tcggatacaa ctagtgagct tgagaaagaa aacgcctctg ctggaattaa
     3661 taatagtgac agcactggtg gcggtaaaag gttaaatacc tctattcgta g
//
LOCUS       DAVLYR010000149         3665 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLYR010000149 DAVLYR010000000
VERSION     DAVLYR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.149"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            24..1520
                     /locus_tag="VYO63_001764"
     CDS             24..1520
                     /locus_tag="VYO63_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEV1672639.1"
                     /translation="MIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDISGAEGY
                     FTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVKWSEDD
                     TTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQQHYVR
                     SDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYGKLEMR
                     AKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNGSGNKT
                     VYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDNHIVRK
                     VYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRNDLQQHQ
                     AEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFGGNTSV
                     DLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            1533..1850
                     /locus_tag="VYO63_001765"
     CDS             1533..1850
                     /locus_tag="VYO63_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1672640.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            1867..2184
                     /locus_tag="VYO63_001766"
     CDS             1867..2184
                     /locus_tag="VYO63_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1672641.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            2194..3549
                     /locus_tag="VYO63_001767"
     CDS             2194..3549
                     /locus_tag="VYO63_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1672642.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
BASE COUNT         1184 a          490 c          796 g         1195 t
ORIGIN      
        1 atagtaacgg acattactca ggtatgattc cttctactgc aattaatgat aatagagcag
       61 atggatatat tggacaagtt attgatatag aacctaatga agattattta gtatctgctt
      121 ttgccaaagt agatattagt ggagcagagg gatattttac cgcaagatgg tttgataata
      181 ataagcaagg agagattgtt caaaataaca ttggagaagc tgttgatcaa acagttaata
      241 caaccgaatg gaaaaaatac atgttttctt tcaattccgg taaacacgat aaagttttga
      301 ttcaattagt gaaatggtca gaagacgata ctacaaaaaa atctaatatt tttattgata
      361 atgtagagat gtatcaactg tctaaaggaa attcatataa gaaaatttgg agagatgatt
      421 ttgatggcga acaattaaat aaaaaatatt ggggatatga attaggatca attcgtggtt
      481 gggaacagca acattatgta agaagtgatg agaatgtttt tctacgttcg ggaaatttag
      541 ttcttagagc tacgaatcgc tctaaggaag atcagtattt taacccaaga aacaatcaca
      601 gaaaggttgt atataattca ggaagtgtta gaacacatgg gaaagtagaa tttctttatg
      661 gtaaattaga gatgcgtgca aaattaccga aaggtcaagg agtttttcct gctttctgga
      721 ctcttggatc ggattttaca cttgatggga agattaatcc tgttcaggga cgaggatggc
      781 cgtcaacagg tgagatagat ataatggaat tagttggtga acgaaattca aatggaagtg
      841 ggaataagac agtttatcaa acgcttcatt atggacagtc ggataatgat gatggaaaat
      901 ttgcaggacg tggtacagcc tttaaattat cgagtggaaa ctttaatgat tcgtatcata
      961 ctttttcaat tgattggtat aaagattata ttgtatggat ggttgacaat catattgtta
     1021 gaaaagtata ttatggatca gataatatag cgaaaaagat ttttaataga cctcagtatg
     1081 ttcagttaaa tttagcgatg ggaggaagtt ggcctggggt agtggacaat aatttatcag
     1141 ggactgaatt tgtagttgat tatgtttctt actctagaaa tgatttacaa caacatcaag
     1201 ctgaagagta ttatagtcaa tctcccaaaa taaatggcgc taaggatata gtaataaaca
     1261 gaggagatgt tcctgattta ttaaaaggaa ttacaactaa tagtggatat gagttagatt
     1321 attctattga aaatgaacct atgtttaata actttggagg gaatacttcg gtagatttat
     1381 taattcgcaa taaaactgaa aaagaaaaga tttcacaaat gcctccagga gtttataatt
     1441 tgcattatac tagtcgtccc aaacatttag attatgagtc taaagttgat cgaaaaacag
     1501 taacattgac aataaaataa aaggagtaag ctatggctaa agtaacaatt atgttagcat
     1561 gtgcagcagg tatgagtaca agtctgctag tgacaaagat gcaaaaggca gcagaagata
     1621 aggggttgga tgcagaaatt tttgcagttc cagctcctga agcagaagaa attgtagcaa
     1681 caaaagaagt aaatgtgttg cttttaggtc ctcaagttcg ctatttacta ggggactttc
     1741 aagaaaaact aaaagataga cagattcctg tggcggttat tccgatgaca gattacggaa
     1801 tgatgaatgg ttctaaagtt ttagatttag ctgaaagttt attagactaa gattgaggag
     1861 aatgttatgg atgaaagtaa tttagaatct gtaatggggc taattatgta tggtggggaa
     1921 gccaaaagta atgctatgga ggctattcag gcagcaaaaa aaggggattt ctctaaagcc
     1981 aatcgaagat tagctgatgc gaatgctgcc ttattacagg cgcataaggc tcaaacagaa
     2041 atgttgacaa gagaggcaca gggggaaaaa acatcaatta gcctcttgat ggtacacgcg
     2101 caagatcatt taatgacgag tctgactttt gttgacttgg cgaaagaagt ggtagaagta
     2161 tacgaacgat ttgaaaaaaa ttaggagttt ggtatgaata caatgttgga taaaatgcaa
     2221 gaaaaacttt ctccaattgc aatgaaagtt gggaatcaga agtttttagt tgctttacgt
     2281 gattcatttg tggggactat gcctgttatt atgacaggtt ccattgcttt gttattaaat
     2341 gcttttctag tggatttacc acaacaattt cacctagaaa gtattacgaa aacctttcag
     2401 tggcttgttg acatcaataa tttggtattc aagggaagta taccaattgt ttccttgttg
     2461 ttcatctatt gcttgggagt gaatatcgct aagatttaca aggttgatac agtttctgct
     2521 ggtttggtat cacttgcttc gtttgtcatt tcgattggaa gtacagttac aaagagtttc
     2581 cctttggcaa atgtaggaga tgtgaaatta gatcaaatct tacaaggaat tgataatcta
     2641 gcatttgatg gtaaaaatct gatggtaact attgggaatg tgattccagg aaaccatatt
     2701 aatgccagag gctactttac agccatgatg attggatttt tagcctctat tattttctgt
     2761 aaagtaatga aaaagaactg ggtaatcaag ttaccagatt cagttccccc tgcgattgct
     2821 aagccgttca cttctatcat tccaggattt atggcgatgt atatagtagc catcttgact
     2881 tatgttttcc atttacttag caatgactta ttgattgatt gggtttacaa ggtgttacaa
     2941 acgccattac taggtttgtc tcaaagtttc tttgcagtta ttctgatgat ctttttgaat
     3001 aagttattct ggttttttgg tctccatgga ggaaatgtat tggctcccat catggaaggg
     3061 ctgtttggag ttgctatgtt agcgaatctg gatgctttcc aaaaaggtga accgattcct
     3121 tatatatgga caagtgggtc ttttggggcg tttgtctggt ttggaggatt aggattagta
     3181 cttgctattt taattttttc aagaaacagt cattatcgaa aagttgctaa acttggtcta
     3241 gcaccagttc tgtttaatat tggtgagccg gtcaattatg gtttaccagt tgtcttgaat
     3301 cctttgctat ttataccatt tgtacttagc ccggttttca tggcaacggt agcttactgg
     3361 gcaacaagct ggggtcttgt ttcacctgtt acacaaaacg ttacttgggt aatgcctcca
     3421 attttatatg gtttcttctc tacagcattt gattggcgtg ccatcatctt atcagttgtt
     3481 tgtttgatta ttagtgtctt gacttatttc ccatttgtga aaatggcaga taaaactgaa
     3541 ttgagttaat ggtcttactt gcgttttttg tgataaaata gaaatagatt acgtgtaaga
     3601 tgagttaggt gtaggatttc ttatctccac taaaattgtt attagacaaa ggtttatagt
     3661 ttatt
//
LOCUS       DAVLYR010000150         3634 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLYR010000150 DAVLYR010000000
VERSION     DAVLYR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.150"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1118
                     /locus_tag="VYO63_001768"
     CDS             <1..1118
                     /locus_tag="VYO63_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1672643.1"
                     /translation="NFFIAERFSISRRGMVYFLTLEGISLYLLNFLVKKYWKHVFFNL
                     KNSKKILLLTVTKNMEKVLDKLLESDELSWKLVAVSVLDKSDFQHDKIPVIEKEKIIE
                     FATHEVVDEVFVNLPGESYDIGEIISRFETMGIDVTVNLKAFDKNLGRNKQIHEMVGL
                     NVVTFSTNFYKTSHVIAKRIIDIMGALVGLILCGLVSIVLVPLIRKDGGSAIFAQTRI
                     GKNGRQFTFYKFRSMCVDAEAKKRELMEQNTMQGGMFKVDDDPRITKIGRFIRKTSLD
                     ELPQFYNVLKGDMSLVGTRPPTVDEYVQYTSEQKRRLSFKPGITGLWQVSGRSKITDF
                     DDVVKLDVAYIDNWTIWKDIEILLKTVKVVFMRDGAK"
     gene            1122..1865
                     /locus_tag="VYO63_001769"
     CDS             1122..1865
                     /locus_tag="VYO63_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV1672644.1"
                     /translation="MRDRIQLLGVTIDLLTMNETIDIVEQYVLEKRPLHLMGVNADKI
                     NQCHTDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEVLQDMLKVFKRDYPNLIVIGHRNGYFSEEDEQAIQEDIREKNPDFVF
                     IGITSPKKEYIIQKFMDSGVNSVFMGVGGSFDVLSGHIQRAPLWMQKSNLEWLFRVAN
                     EPKRLFKRYFVGNISFIGRVLKAKRGVKY"
     gene            1862..2686
                     /locus_tag="VYO63_001770"
     CDS             1862..2686
                     /locus_tag="VYO63_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV1672645.1"
                     /translation="MNQTEMIRLIQKVELDAIKEFKKICEENDIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREAYDKLPSVFKDRIIAGKYQVLTYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSVLRKIYFGKVYWYRFLASLGTTYVGDH
                     VDMHSTKQKLIIGFFKKLGFAKLFPQNSVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDTFFKVPTEYDRYLKRLYGENYLYEEPSDDEKKSHLGGH"
     gene            2688..3566
                     /locus_tag="VYO63_001771"
     CDS             2688..3566
                     /locus_tag="VYO63_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1672646.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNYNAKQIVIIDNFSNNGTG
                     EKLQELYESDLEIDVLINHENAGFARGNNVAYQFAKEKYNPDFMVIMNNDIEIETENF
                     EKIVTDIYREEKFHLLGPDIFSTTYQLHQNPKRLTHYTYGEVKALNEKFKKGSQVSLA
                     LKIKCWLKASKVLRTAIYQNRRKKGSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIRVLHHQNVATNQVYTNLLEKTLFSNKCNF
                     KSTSYFLKLMKENEDV"
BASE COUNT         1242 a          483 c          740 g         1169 t
ORIGIN      
        1 taaacttttt tatagcggaa cggtttagta tctctagaag aggaatggta tacttcttaa
       61 ctttagaagg aatatcctta tacttgttaa atttcttagt aaagaaatat tggaagcatg
      121 tgttttttaa tctaaaaaat agcaagaaaa ttttactgtt aacagtaacg aaaaatatgg
      181 aaaaagttct tgataaattg ctagaatctg atgaactttc atggaaattg gtagcagtaa
      241 gtgttttgga taaatctgat tttcaacatg ataaaatacc tgtaattgaa aaggaaaaaa
      301 ttattgaatt tgcaacgcat gaagttgtgg atgaggtgtt tgtcaatctt ccaggagaga
      361 gctacgatat tggagaaatt atctctaggt ttgagacaat ggggatagat gtaactgtaa
      421 atcttaaagc atttgataag aatttgggtc gcaataaaca aattcatgag atggtaggat
      481 tgaatgtagt cactttctct acaaattttt ataaaactag tcatgtaatt gctaagcgga
      541 ttattgatat catgggtgca ttggtagggc tgatactatg tggtttagtc agtattgtat
      601 tggttccttt gattcgaaag gatgggggct ctgctatttt tgctcagacg cgtataggaa
      661 aaaatggtcg tcagttcact ttttataagt ttcgctctat gtgtgtagat gccgaggcga
      721 aaaaaagaga actcatggaa caaaatacca tgcagggtgg aatgtttaag gtggacgatg
      781 atcctcgtat cacgaaaatt ggtcgtttta tacggaagac tagcttggac gagttaccac
      841 agttttataa tgttctaaag ggagatatga gtttggttgg cacacggcca cctacagtgg
      901 acgagtatgt tcagtatact tcagaacaga aacgtcgact cagctttaaa cctggtatta
      961 caggtttatg gcaggttagc ggccgtagta aaataaccga ttttgacgat gttgtaaaat
     1021 tagatgtggc ttatattgat aattggacaa tctggaaaga tattgaaatt ttgcttaaaa
     1081 ctgttaaagt tgtatttatg agagatggag cgaagtgagg aatgagggat agaatccaac
     1141 ttttaggtgt aacaattgat ttgcttacga tgaatgaaac gatagatatt gtagaacaat
     1201 atgtattaga aaaaagacca ctacacttga tgggggtgaa tgctgataaa attaatcagt
     1261 gtcatacaga tgagaaaatc aaaaaaatcg ttaatgagtc aggaatcatt aatgcggatg
     1321 gagcatcagt tgttcttgca agtaagtttt taggaacgcc tgttcctgaa cgagtagcgg
     1381 gtattgattt gatgcaatgt cttttagagt tgtcaaataa aaaaggatat tcagtttact
     1441 tttttggagc aaaagaagaa gttttgcaag atatgctcaa agtatttaag agagattatc
     1501 caaatttgat agttattgga cacagaaatg gctatttttc tgaagaggat gaacaagcta
     1561 ttcaagaaga tattcgtgaa aagaaccctg attttgtgtt tattggaatt acgtctccta
     1621 aaaaagaata tattattcaa aaatttatgg atagtggcgt caattcggta tttatgggag
     1681 ttggcggtag ttttgatgtc ttgtctggtc atatccaacg agcacctcta tggatgcaaa
     1741 agtcaaattt agagtggtta ttccgtgtag ctaatgagcc taaacgtctc tttaaacgtt
     1801 attttgtagg gaatatttca ttcataggaa gagttttaaa agcaaaaaga ggtgtaaaat
     1861 attgaaccag acagagatga ttcgcttaat tcaaaaagtt gaattagatg ctataaaaga
     1921 gtttaaaaaa atctgtgaag agaatgatat agattttttc ctccgcggtg gtagtgtact
     1981 tggtgcggtc aaatacgacg gctttattcc atgggatgat gatatggata tcgctgtccc
     2041 tcgtgaagca tacgacaaac ttccaagtgt tttcaaagat agaattatcg ctgggaaata
     2101 tcaggttctt acttatcaat actgtgatac gttgcattgc tactttcctc gactattcct
     2161 tttagaagat gaaagaaaac gtttgggctt gccacgaaat accaatctag gattgcattt
     2221 gattgatatc attcctttag atggagcacc aaatcattcg gttttaagaa agatttactt
     2281 tggcaaagta tactggtatc gttttttagc aagtttagga acaacttatg ttggcgacca
     2341 tgtggatatg cattccacta agcaaaaact aattattggt ttctttaaaa aactaggatt
     2401 tgcaaaacta tttcctcaaa attctgtata cagacgcttg gataatctct ataaaaagta
     2461 tgattggaaa aagcagaagt atgcggggac tatcaatgct tctttatttg ctaaagaagt
     2521 tatgccagta gagatttggg gagaaggagt agagaagcct tttgaggata ctttctttaa
     2581 agttccaacg gagtatgatc gctacctgaa aagactttac ggagaaaact atctttacga
     2641 agagcctagt gatgatgaaa agaaatcgca tttaggagga cactaatttg ttttgttata
     2701 ttattttgca ttacaaagtc ttagaagaaa ctatttcttg tgttaaatct ataaaagaag
     2761 gcaattataa tgcaaagcaa atcgttatta ttgataattt ttctaataat ggtactggtg
     2821 aaaaactaca agagctttat gaatcagatt tagaaattga tgttttgatt aaccatgaaa
     2881 atgctggttt tgctcgtgga aataatgtgg cttatcaatt tgctaaggaa aagtataacc
     2941 ccgatttcat ggttatcatg aataacgata ttgagataga aacagaaaat tttgaaaaaa
     3001 ttgtgacaga tatctatcgt gaggaaaaat tccatttgct cgggccagat atcttctcga
     3061 ctacttacca acttcaccaa aacccaaaac ggttgacaca ttatacttat ggagaagtta
     3121 aagctctaaa tgaaaaattt aaaaaaggga gccaagttag tctagcttta aaaatcaaat
     3181 gttggttgaa agctagtaaa gttcttcgaa cagcaatcta tcaaaataga cgtaaaaaag
     3241 gatcagtaga ctatagaaaa caggtagaaa acccaattct tcatggttct tttattgtat
     3301 attcgagaga ttttatcgaa aaagaggagt atgcttttaa ccctaacacc ttcttctact
     3361 atgaaacaga gatattagat tatgaagctg aattaaaagg atacaagaga atttatacac
     3421 ctaaaattag agttttgcac catcaaaatg ttgcaactaa tcaagtttac acgaacttgt
     3481 tagaaaaaac cttgttttca aataaatgca actttaaatc caccagttat tttttgaagt
     3541 tgatgaaaga aaacgaggat gtttaaatga gttatttatt tttactttgc cttacattat
     3601 tcttattgac tatattctat ttctttgctt ttat
//
LOCUS       DAVLYR010000151         3601 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLYR010000151 DAVLYR010000000
VERSION     DAVLYR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.151"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            58..285
                     /locus_tag="VYO63_001772"
     CDS             58..285
                     /locus_tag="VYO63_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672647.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            573..3470
                     /gene="nanA"
                     /locus_tag="VYO63_001773"
     CDS             573..3470
                     /gene="nanA"
                     /locus_tag="VYO63_001773"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1672648.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKEAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1222 a          610 c          825 g          944 t
ORIGIN      
        1 agctatgcga aataacagat agagagaagg gattgaagct tagaaaaggg gaataatatg
       61 atatttaagg cattcaagac aaaaaagcag agaaaaagac aagttgaact acttttgaca
      121 gtttttttcg acagttttct gattgattta tttcttcact tatttgggat tgtccccttt
      181 aagctggata agattctgat tgtgagcttg attatatttc ccattatttc tacaagtatt
      241 tatgcttatg aaaagctatt tgaaaaagtg ttcgataagg attgagcagg aagtatggta
      301 taaatagcat aagctgatgt ccatcatttg cttataaaga gatattttag tttaattgca
      361 gcggtgtcct ggtagataaa ctagattggc aggagtctga ttggagaaag gagaggggaa
      421 aattggcacc aatttgagat agtttgttta gttcattttt gtcatttaaa tgaactgtag
      481 taaaagaaag ttaataaaag acaaactaag tgcattttct ggagtaaatg tcttatttca
      541 gaaatcggga tatagatata gagaggatca gtatgaatcg gagtgttcaa gaacgtaagt
      601 gtcgttatag cattaggaaa ctatcggtag gagcggtttc tatgattgta ggagcagtgg
      661 tatttggaac gtctcctgtt ttagctcaag aaggggcaag tgagcaacct ctggcaaatg
      721 aaactcaact ttcgggggag agctcaaccc taactgatac agaaaagagc cagccttctt
      781 cagagactga actttctggc aataagcaag aacaagaaag gaaagataag caagaagaaa
      841 aaattccaag agattactat gcacgagatt tggaaaatgt cgaaacagtg atagaaaaag
      901 aagatgttga aaccaatgct tcaaatggtc agagagttga tttatcaagt gaactagata
      961 aactaaagaa acttgaaaac gcaacagttc acatggagtt taagccagat gccaaggccc
     1021 cagcattcta taatctcttt tctgtgtcaa gtgctactaa aaaagatgag tacttcacta
     1081 tggcagttta caataatact gctactctag aggggcgtgg ttcggatggg aaacagtttt
     1141 acaataatta caacgatgca cccttaaaag ttaaaccagg tcagtggaat tctgtgactt
     1201 tcacagttga aaaaccgaca gcagaattgc caaatggtcg agttcgcctg tatgttaatg
     1261 gtgttttatc tcgaacaagt ctgaaatctg gcaatttcat taaagatatg ccagatgtaa
     1321 cgcatgtgca aatcggagca accaagcgtg ccaacaatac ggtttggggg tcaaatctac
     1381 agattcggaa tctcactgtg tataatcgtg ctttaacacc agaagaggta caaaaacgta
     1441 gtcaactttt taaacgctca gatttagaaa aaaaactacc tgaaggagcg gctttaacag
     1501 agaaaacgga catattcgaa agcgggcgta acggtaaacc aaataaagat ggaatcaaga
     1561 gttatcgtat tccagcactt ctcaagacag ataaaggaac tttgatcgca ggtgcagatg
     1621 aacgccgtct ccattcgagt gactggggtg atatcggtat ggtcatcaga cgtagtgaag
     1681 ataatggtaa aacttggggt gaccgagtaa ccattaccaa cttacgtgac aatccaaaag
     1741 cttctgaccc atcgatcggt tcaccagtga atatcgatat ggtgttggtt caagatcctg
     1801 aaaccaaacg aatcttttct atctatgaca tgttcccaga agggaaggga atctttggaa
     1861 tgtcttcaca aaaagaagaa gcctacaaag aaatcgatgg aaaaacctat caaatcctct
     1921 atcgtgaggg agaaaaggaa gcttatacca ttcgagaaaa tggtactgtc tatacaccag
     1981 atggtaaggc gacagactat cgcgttgttg tagatcctgt taaaccagcc tatagcgaca
     2041 agggtgatct atacaagggt gaccaattac taggaaatat ctacttcaca acaaacaaaa
     2101 cttctccatt tagaattgcc aaggatagct atctatggat gtcctacagt gatgacgacg
     2161 ggaagacatg gtcagcgcct caagatatta ctccgatggt caaagccgat tggatgaaat
     2221 tcttgggtgt aggtcctgga acaggaattg tacttcggaa tgggcctcac aagggacgga
     2281 ttttgatacc ggtttatacg actaataatg tatctcactt aaatggctcg caatcttctc
     2341 gtgtcatcta ttcagatgat catggaaaaa cttggcatgc tggagaagcg gtcaacgata
     2401 accgtcaggt agacggtcaa aagatccact cttctacgat gaacaataaa cgtgcgcaaa
     2461 atacagaatc aacggtggta caactaaaca atggagatgt taaactcttt atgcgtggtt
     2521 tgactggaga tcttcaggtt gctacaagta aagacggagg agtgacttgg gagaaggata
     2581 tcaaacgtta tccacaggtt aaagatgtct atgttcaaat gtctgctatc catacgatgc
     2641 acgaaggaaa agaatacatc atcctcagta atgcaggtgg accgaaacgt gaaaatggga
     2701 tggtccactt ggcacgtgtc gaagaaaatg gtgagttgac ttggctcaaa cacaatccaa
     2761 ttcaaaaagg agagtttgcc tataattcgc tccaagaatt aggaaatggg gagtatggca
     2821 tcttgtatga acatactgaa aaaggacaaa atgcctatac cctatcattt agaaaattta
     2881 attgggaatt tttgagcaaa aatctgattt ctcctaccga agcgaaagtg aagcgaacta
     2941 gagagatggg caaaggagag atgggcaaag gagttattgg cttggagttc gactcagaag
     3001 tattggtcaa caaggctcca acccttcaat tggcaaatgg taaaacagcg actttcctaa
     3061 cccagtatga tagcaagacc ttgttgtttg cagtagataa ggaagatatc ggacaggaaa
     3121 ttattggtat agctaaagga agcatcgaaa gtatgcataa tcttcctgta aatctagcag
     3181 gtgccagagt tcctggcgga gtaaatggta gcaaagcagc ggtgcatgaa gttccagaat
     3241 ttacaggggg agttaatggt acagagccag ctgttcatga aatcgcagag tataagggat
     3301 ctgattcgct tgtaactctt actacaaaag aagattatac ttacaaagct cctcttgctc
     3361 agcaggcact tcctgaaaca ggaaacaagg agagtgacct cctagcttca ctaggactaa
     3421 cagctttctt ccttggtctg tttacgctag ggaaaaagag agaacaataa gagaagaatt
     3481 ctaaacattt gattttgtaa aaatagaagg agatagcagg tttttaagcc tgctatcttt
     3541 ttttgatgac attcaggctg atacgaaatc ataagaggtc tgaaactact ttcagagtag
     3601 t
//
LOCUS       DAVLYR010000152         3600 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLYR010000152 DAVLYR010000000
VERSION     DAVLYR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.152"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            114..1523
                     /locus_tag="VYO63_001774"
     CDS             114..1523
                     /locus_tag="VYO63_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1672649.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1541..2836
                     /locus_tag="VYO63_001775"
     CDS             1541..2836
                     /locus_tag="VYO63_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1672650.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2841..3149
                     /locus_tag="VYO63_001776"
     CDS             2841..3149
                     /locus_tag="VYO63_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1672651.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3146..3454
                     /locus_tag="VYO63_001777"
     CDS             3146..3454
                     /locus_tag="VYO63_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1672652.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEVHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1216 a          607 c          701 g         1076 t
ORIGIN      
        1 gttcaagaaa tgaggcaaac acaagatatg tatcaacaat ttttaaaaat gtctagacaa
       61 attttaaaat tatgctatac tttatgtata gaataataga aaggaggttg agcatgaaaa
      121 aaaagttagt atttcctaat ctattttggt ggggagctgc ttctagcgga cctcagacag
      181 aaggtcaata tggaaaagta catgaaaatg tgatggacta ctggttcaaa acgcatccag
      241 aagatttttt cgataatgtc ggacctcttg tagccagtaa cttttttcat acttacaccg
      301 aagatttcca cttgatgaag gaaattggag ttaattcttt ccgcacttcc atccaatgga
      361 gtcgactcat caagaattta gagacaggtg agcctgatcc aaaaggtatt gctttctaca
      421 atgccatcat tgaagaagct aaaaagaacc agatggatct tgtgatgaat ttacatcatt
      481 ttgatttacc agtggaactt cttcaaaaat acggtggttg ggaaagcaaa catgtagtgg
      541 agttattcgt gaagtttgcc aagactgctt tcacatgctt tggagataag gttcattact
      601 ggacaacttt caatgagcca atggtcattc cagaagcagg atacttatat gctttccatt
      661 atccaaatct aaaaggaaag ggaaaagagg ccgtacaagt catctataat ctaaaccttg
      721 ctagtgcaaa agtgattcaa ctatatcgct cattagaact tgatggaaag attgggatta
      781 ttttaaactt gacacctgct tatccaagaa gtaattctcc agaagactta gaagcaagtc
      841 gatttacaga tgacttcttt aacaaagtct tcttgaatcc agctgttaaa ggaactttcc
      901 cagaaagatt ggtaaaacag ctagagagag atggcgtgtt atggagtcat accgaaaaag
      961 agcttcaact gatgaaatca aatacggttg attttcttgg agtaaactac taccatccaa
     1021 aacgtgttca agcacaagca aatcctgaag aatatcagac gccctggatg ccagaccaat
     1081 acttcaaaga gtatgaatgg ctggagcgtc gcatgaatcc atatcgtggt tgggaaattt
     1141 ttccgaaagc catttatgat attgctatga ttgtgaagga agaatatggt aatatcccat
     1201 ggtttatcag tgaaaacgga atgggtgttg aaaacgaagc acggtttatc gatgaaaatg
     1261 gagttatcga tgacgtgtat cgtattgaat tttatgaaga acatttaaga tggctacata
     1321 aagccattga agagggaagt cactgttttg gataccacgc ttggaccgca tttgattgct
     1381 ggtcttggaa taatgcatat aagaatcgtt acggatttat ctccgttgat ttagaaacgc
     1441 aaaagagaac catcaagagc tcaggaagat ggtatcgcaa agtaagtgac aataacggtt
     1501 ttgaagtaga aattgaggag taaagagagg agatattcat atgaaaaact caaaatttat
     1561 agaccaattt gccacctttg ctggtaaact agggaaccaa attcatttaa aaaccctaag
     1621 agatgcattc gtaacagtaa tgccattata tattttggca ggtttaatcg ttcttttgaa
     1681 caacacggta tttaagtgga ttttccaagg ggatacatta acaagattcc aatattgggg
     1741 aataacaatt gcaaacggta ctttaagtat ttcaggtatg attattgctg taatggttgg
     1801 ttatttctta gctaaaaaca gagatttcga aaacccgtta gcagcatcaa tgctatcatt
     1861 agtttcttta attgtgatga tgccaaatac agtttctgta gttcctgacg gagcaaaaga
     1921 tgcggtaaac atttcaggtg ttctttcatt caacaacaca ggtacaggcg caatgttcgc
     1981 cggggttatc gtagcgatta ttgcaacaga attattcatt gaattatcaa acgttaaagc
     2041 tttacaaatg aaccttggtg aaaatattcc accagctgtt agtagatcat ttagcgtatt
     2101 acttccagtc atgaccgtca tctccttatt tggggttgtt tcagcattat tattcaatat
     2161 aactggaatg aacttaatct caatcattac aatctttatt caagaaccaa ttcgtcatat
     2221 tggtacaagc ttaatcgggg tcattattat ttactcttta ggaaatatgt tatggctatt
     2281 tggtattcac caagcagtta tttacagtgc catcctagaa ccattactat taattaacat
     2341 tactgaaaac atcactgcag caaataatgg acaagccatt ccacacatca tcaacctatc
     2401 acaaatacaa acattcgctt taatgggtgg tagtggatct acattatgtt tattaatagc
     2461 aacattctta gtgagtcgca atgctgtctc taaaaacgtg gctaaattat cttttggacc
     2521 tggtatcttc aatatcaatg aaccagtatt attcggttac ccaatcgttt ataacatttc
     2581 attagctatt ccatttatca cagttccagt ccttggtatt ttaatcagct acttagcaac
     2641 agttacagga ttcatgagtc ctgcatttat acaagttcct tggactacac cagtattctt
     2701 aaatgcatgg ttagcaacag caggggacgt gagagcagtt ctagttcaat tcatcatctt
     2761 tgcacttgga gttcttctat acattccatt tatcaaagtt aatgacaaag ttgttgaaca
     2821 agaaatggaa ggttaagatt atgaaacaaa ttttattagt atgtaatgcg ggaatgtcga
     2881 caagtatgct tgttaaaaaa atgcaacaaa gtgcgactga acgtgggatt gaaatttcta
     2941 tccaagcaaa atcaatgaca gaggcgaaaa agaatatcca tgaagcagat gtcattctaa
     3001 ttgggccaca aattcgttat gagttacttg ctgtgaaaga aattgcagga aatatccctg
     3061 tagataccat tgatatgcgg gattacggaa tgatgaatgg agcaaaagtt ctggaacaag
     3121 cacttgcatg gataggagaa ataagatgac agaagaaatg gaatatatct gttttcagct
     3181 cattgccaat agtggagcag ccaaatcatc ctttattgaa gccatccaac tagctaaggc
     3241 aggaaattta aaagaagcga aaattaaagt tgaagaagcg gaagactcct tagtagaagt
     3301 gcacaagatt cactctaatc tcatacaaaa agaagctact ggagaaaaaa taggattctc
     3361 cttgctattt atgcatgcag aagaccaaat ggcttcaacg gagattattc agttgctatc
     3421 taaagaattc atcgatctct atcaaaataa ataggaacac tgtcgttccc atctgtcttt
     3481 attaatgacg gctacattgt aggaacctcc aaaagttgga caaaatgatt gaaaggattg
     3541 gatcctgcat tacacaagat ccaatccttt tcttttactc tagtttttat aatatgttga
//
LOCUS       DAVLYR010000153         3541 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLYR010000153 DAVLYR010000000
VERSION     DAVLYR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.153"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(237..848)
                     /gene="rpsD"
                     /locus_tag="VYO63_001778"
     CDS             complement(237..848)
                     /gene="rpsD"
                     /locus_tag="VYO63_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1672653.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1043..2095)
                     /locus_tag="VYO63_001779"
     CDS             complement(1043..2095)
                     /locus_tag="VYO63_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1672654.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2092..2790)
                     /locus_tag="VYO63_001780"
     CDS             complement(2092..2790)
                     /locus_tag="VYO63_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672655.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQI"
     gene            complement(2957..>3541)
                     /locus_tag="VYO63_001781"
     CDS             complement(2957..>3541)
                     /locus_tag="VYO63_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1672656.1"
                     /translation="PWSDNGDAKNPALSPLGENVKTKGQYFYQLALDGNVAGKEKQAL
                     IDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSN
                     SGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPN
                     TGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          977 a          791 c          684 g         1089 t
ORIGIN      
        1 ttcgctcaca ataagagaga acttttttac gaaaatctat tgaatatgcc ataaaagatt
       61 ataccacatt gtgtactatt tttggttcat tttactatat cactttggac tatatcggtc
      121 tagttatgtc tcaacgatta tagctggata ctaagagaaa tcaaaaaagc actaagggag
      181 aacgtcccaa agtgcttaat atcaaggcct caaagcctgt cttattcaat aaaatattac
      241 aacatcttgt tgtagaattc aacgacaagt gcttcgttga tttctgggtt gatttcgtcg
      301 cgttctggca agcgagtcaa tgaaccttcc aacttttcag cgtcgaatga tacgaatgct
      361 ggacgtccaa gagtagcttc tactgcttca aggattgctg gaactttcaa tgatttttca
      421 cgaactgaga tcacttgacc tggagttacg cggtatgatg ggatatcaac gcgtttcccg
      481 tcaacaagga tgtgaccgtg gtttacgaat tgacgagctt gacgacgagt agtcgcgaga
      541 ccaagacggt aaacaacatt atccaaacga cgttccaaaa gaagcataaa gttgaaacct
      601 aggattccac ctttgatttt tgtagcttgt acgaacaagt tacggaattg tttttcacct
      661 acaccgtaag tgaaacgaag tttttgtttt tcagccaatt gcaaaccata ttctgacaat
      721 ttagaacggt tgtttggtcc gtgttgtcct ggtacgtagt tacgacgtgc caattcttta
      781 cctgtacctg taagtgaaag gccaaggcga cgagcttgtt tccaagatgg tcctgtataa
      841 cgtgacatat gaatgtcctc ctgatataaa taatatttcg gtggaaatag tcacttagaa
      901 agccctgatt cgtgcagatg cccttcgcct aaacagccaa ggttacttgt cataagacac
      961 ctgttgacga gcttcatgct ttcctgctga tatttcacac aaaatccatt ttaccatgaa
     1021 tagctggatt tgtaaagggg ttttaggctt tattttcact accagagagg ttgagaacga
     1081 gggtaaaggt acttcctaga ccgtactggc tgctgactgt gatttcccca cccaattgat
     1141 gggccaattc acgcgcaatc gcaagtccta atccatgacc acctgtcttc atgttacgcg
     1201 aagtttcgac acgataaagg cgtttgaaaa tattttccaa atcctctggg gcaatgccct
     1261 gcccttcatc ggtcacactg attgaaagct ggtccttctc cagcttagcc accacttcca
     1321 gcttggttcc tggagcagaa tatttaaaag cgttatcgac cagattcacc aagatacgag
     1381 aaagcttagc ataatctccc tcaatccggg cagactctgg gattacctgc aagtggacat
     1441 ctcttctctc ctgctcaatc aaaaactgaa attcactcat gcactcaatt aagagcttgt
     1501 ccagaaaaat actgtcttta ctggtagttt ccacctgatt tctagctgtg tttagggtca
     1561 aaaaattcaa ctcctcaacc agtttattga gcctctccgt ctggcgtcca atggttgcta
     1621 gataatgagc ttgctccgac tccttgataa tcccatccaa aatcccttct accgtcgctt
     1681 ggatcgaagt gataggggtc ttaatatcat gcgacaactg ggcaatcatc aagccctttt
     1741 ctcgttcgct ttcttccaag gaatcaaagc ttacctgcaa atcatgggac atctcattaa
     1801 aagtttgccc taattgctga aattctacag gaccttgaac ctccaaattt gaaggaaaat
     1861 ccttggccgc tacccgcttg gcatgctcct tgagtttgcc caacgacgta aagactggca
     1921 ataggagaaa gagactaatc ccagcaccga caaggctggc aacgatggtc atcccaagca
     1981 aaaagtaaat ctcgcctttc gcaatcagca ttttttgaac agcccaaaaa acaaccaaaa
     2041 tggttaagag ggttgaaata atatatccaa ccaaaatata actttttagt ttcatatttg
     2101 tcctctcggt ttctctatct tatatcccaa cccccaaact gtcttaatag tgggagtttg
     2161 gtcactacta tattttgcca gctcctgtcg aagagcatgg atatgcacat tcaaggtatt
     2221 ggtgtcatcc acgtagtctt ctttccagat cttttcatag aggtctgtct tggagaaaac
     2281 tcgctctgga ttgctagcta aaatccacag caattcaaat gatttaacag ttaaatccag
     2341 catttcttct cctatttgaa cttcatgact actatgattc atttttagat tgccaaggga
     2401 aatcagctct gtttcgcccc cacgatgaag gcggcgcaaa atattgtgga cacgcaaaac
     2461 cagctcacgt gggctaaaag gcttagcaat aaaatcatct gctcccaagc tcaggccgta
     2521 aatcttgtcc tgttcactgg tcttagcagt aataaatagg aaaggctgct ctggtgataa
     2581 gtattgaacc tcactgatta aatcataacc atccatccga ggcatcatga catctgtgat
     2641 aatcaaatca atcggttttt tcttgaacag ctctagcgct tccagtccat catgggctac
     2701 caagacctga taacctgcct gaattaagta tctctgatga atatctgtga tttctacctc
     2761 gtcgtcaacg agtaaaattg tctttcccat ctgtctctcc tttgataaaa acagtgctat
     2821 actgctttaa gtataacact atttttattg ttttacgatg attttaatct gctattgaaa
     2881 gaaagtcctg attgctgatt gagttgttaa actaatctta gtcagcccac tatccatcat
     2941 ttatttcctt agctgattag ttttcttctt ttttgttttt caaacctaga ccaccgagta
     3001 aacctgcaag cgcaagccca aggaaaccaa tacttgccat tgatgtttga gtctcaccag
     3061 tatttggtag catagcttta tcctctgaca tcatcgtatc agacatcttg ttagcagaag
     3121 cagccatgtt ttcacctgcc atcgtgttgg tagaacttgt catggtgtca gcaggcatgc
     3181 tatctgtagt acctgtagca tgattgtgat tcatcggagt cacgccagaa ccagagttag
     3241 aaggtgataa tgaaccattt gctgtgtctg atgtttcttt aacatttatc ttaatagtga
     3301 cttttttagt tgctacgatg ttgtccaaat ctggtttacc gtctttgtta ccatagacat
     3361 tgactgtagc gctgtaagtt tgagtaccat ttgctcggaa ctggtcaatg agcgcttgtt
     3421 tttctttgcc agctacattt ccgtccaagg ctaattgata gaagtattga cctttggtct
     3481 tcacgttttc acctagtgga gatagagctg ggtttttagc gtcgccgtta tctgaccatg
     3541 g
//
LOCUS       DAVLYR010000154         3508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLYR010000154 DAVLYR010000000
VERSION     DAVLYR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.154"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            18..2143
                     /gene="blpA"
                     /locus_tag="VYO63_001782"
                     /pseudo
     CDS             18..2143
                     /gene="blpA"
                     /locus_tag="VYO63_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
     gene            2154..>3508
                     /locus_tag="VYO63_001783"
     CDS             2154..>3508
                     /locus_tag="VYO63_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1672657.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGIFKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HS"
BASE COUNT         1164 a          720 c          575 g         1049 t
ORIGIN      
        1 tatgaaagga attgccaatg acttcttata aacgtacatt tgttcctcaa atagatgcga
       61 gagactgcgg tgttgctgcc ttagcctcga ttgctaaatt ctatggttca gatttttctc
      121 tagctcactt gagagaactt gcaaagacca ataaagaagg gacgactgct cttggcattg
      181 taaaagccgc tgatgaaatg ggctttgaaa caagacctgt tcaagcagat aaaacgctct
      241 ttgacatgag tgatgtcccc tatccattta tcgttcacgt taacaaagaa ggaaaactcc
      301 aacattacta tgttgtctat caaacaaaga aagactatct gattattggt gatcctgacc
      361 cttctgtaaa aatcactaaa atgtcaaaag aacgcttttt ctctgaatgg actggagtag
      421 ctatttttct agctcccaaa cccagctatc aaccccataa agataaaaag aatggtctac
      481 taagcttcct tcctctgatt ttcaaacaaa aatctctcat tgcttacatt gttctctcaa
      541 gcttattggt cactattatc aatatcggtg gttcttatta tctccaagga atcttagatg
      601 aatacatccc aaatcagatg aaatcaactt taggaatcat ctcagttggt ctggttatca
      661 cctatatcct ccaacaagtc atgagcttct ccagagatta agcattgatg tgattttatc
      721 ctatattcgc catatttttg aacttcccat gtctttcttt gcgacacgtc gtacaggaga
      781 aatcatttca cggttcacag atgctaactc tattatagat gccttggctt ctaccattct
      841 ttctcttttt ctggatgttt ctattctgat tcttgtaggg ggcgtcttac tggcacaaaa
      901 ccctaatctc ttcctccttt ctcttctttc cattcctata tacatgttca tcatcttttc
      961 ttttatgaaa cctttcgaaa aaatgaacca tgatgtcatg caaagtaatt ctatggttag
     1021 ctctgccatt atcgaagata tcaacgggat tgaaactata aagtcgctca cgagtgaaga
     1081 aaatcgctat caaaatatag acagcgaatt tgtagattat ttggaaaaat cctttaagct
     1141 cagtaaatat tctattttac aaacgagttt aaagcaggga acaaaattag ttctgaatat
     1201 ccttatccta tggtttggcg ctcaattagt catgtcaagt aaaatttcta tcggtcagct
     1261 gattaccttt aacacacttt tttcttactt tacaactcct atggaaaata ttatcaacct
     1321 ccaaaccaaa ctccaatctg cgaaggtcgc taataaccgt ttgaacgaag tctatctagt
     1381 cgaatctgaa tttcaagttc aagaaaaccc tgttcattca cattttttga tgggcgatat
     1441 tgaatttgat gacctttctt ataagtatgg ttttggacga gataccttaa cagatattaa
     1501 tctcacgatt aaacaaggag ataaggttag cctagttgga gttagtggtt ctggtaaaac
     1561 aactttagcc aaaatgattg tcaatttctt tgaaccctac aaagggcata tttccatcaa
     1621 tcatcaggat attaaaaaca ttgataaaaa agtcttgcgc cgtcatatta attacctacc
     1681 ccaacaagcc tatatcttta atggctctat tttggaaaac ttaaccttgg gcggtaatca
     1741 tatgattagt caagaagata ttctaaaagc ttgtgaagta gctgaaatcc gtcaagacat
     1801 tgaaagaatg cctatgggct atcaaactca gctctctgat ggagctggtc tatcaggagg
     1861 acagaagcaa cgaatcgctc tcgctcgtgc tcttttaact aaatctcctg ttttaatact
     1921 agatgaagct actagcggtc ttgatgtctt gactgagaaa aaagttatag ataatcttat
     1981 gtccctaact gataaaacca ttctctttgt agcccatcgt ctcagtatag ccgaacgaac
     2041 caaccgtgtc attgttcttg accaggggaa aatcattgaa gttggtagcc accaagagtt
     2101 aatgcaggcg caaggtttct accatcatct attcaataaa taaggagaat gtcatgaatc
     2161 ctaatctttt tagaagcgtc gagttttatc agagacgtta ccataactat gcgacagtgt
     2221 taattatacc tctttcatta ctatttactt tcatcttgat tttctccctt gttgccacaa
     2281 aagaaattac tgtcacttcc caaggagaaa tcgcccctac aagtgtcatt tcatctattc
     2341 agtcaaccag tgataatcct atccttgcta atcatttagt ggcaaatcaa gtagttgaaa
     2401 aaggggactt actcatcaaa tactctgaaa caatggaaga aagtcagaaa actgccttag
     2461 caactcaatt acaaagactt gagaagcaaa aagaaggact tggaattttc aaacaaagct
     2521 tagaaaaagc gactgatctt ttttctggcg aggatgaatt tggctaccat aataccttta
     2581 tgaattttac taaacaatcc catgatattg aactgggtat ctcaaagact aacactgaag
     2641 tttcaaatca agctaatctt accaatagca gttcatcagc catcgaacaa gaaattacaa
     2701 aagttcaaca acaaattgga gaatatcaag agctgagaga tgctatcgta aataaaagag
     2761 ctcgcttacc aactggcaat ccgcaccagt caattttaaa tcgttatctt atagcctctc
     2821 aagaacaaac acaaggaacg gcagaggagc catttttatc tcaaattaat caaagtattg
     2881 caggtctgga atcatctatc gcaagcctca aaattcagca agctggtatc ggaagtgtag
     2941 caacttatga taatagttta gcaaccaaaa ttgaagtact ccgcactcag tttttacaaa
     3001 cagcctcaca gcaacaacta accgtggaga atcaattaac agaattaaaa gtacaactag
     3061 atcaagcgac acagcgctta gaaaataata ccttaaccgc tccaagtaaa ggtatcgttc
     3121 atctgaacag cgaatttgaa ggtaaaaata gaattccaac tggtacagaa attgctcaaa
     3181 tattccctgt catcacagat acaagagaag tactaatcac ttactacgta tcttctgact
     3241 atctacctct actagataaa ggacaaactg taagattaaa actggagaag attggaaatc
     3301 acggcaccac catcatcggc caacttcaga caattgatca aactcctacc agaacagagc
     3361 aaggaaatct ctttaaatta accgctcttg caaaactatc taacgaggat agtaaactca
     3421 tccaatatgg cttacaaggt cgcgtcacta gtgtaactac aaagaaaaca tattttgatt
     3481 atttcaaaga taaaatttta acccattc
//
LOCUS       DAVLYR010000155         3480 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLYR010000155 DAVLYR010000000
VERSION     DAVLYR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.155"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..993
                     /locus_tag="VYO63_001784"
     CDS             <1..993
                     /locus_tag="VYO63_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672658.1"
                     /translation="IQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTLRALVGF
                     LDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKKVAPDVI
                     KAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPLSNLDAK
                     LRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPVEIYHNS
                     QTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQDFILKG
                     TIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVLQF"
     gene            1005..2696
                     /locus_tag="VYO63_001785"
     CDS             1005..2696
                     /locus_tag="VYO63_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1672659.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            2699..3409
                     /locus_tag="VYO63_001786"
     CDS             2699..3409
                     /locus_tag="VYO63_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1672660.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1062 a          510 c          662 g         1246 t
ORIGIN      
        1 attcaaatta aatatggtga ttttgttgca attgataatc tgaatttaga tatacatgaa
       61 ggggaattct ttacatttct tgggccttca ggatgtggta aatcaactac tttgagagca
      121 ttggtaggtt ttttagatcc atcatcagga agtattgaag ttaatggaac agatgtcact
      181 catttggaac ctgaaaagcg tgggattggt attgtatttc aatcttatgc gctatttcca
      241 actatgactg tttttgataa tattgcattt ggtttaaaag ttaagaaggt agctccagat
      301 gttattaaag ctaaagtatc agcagtggca gcaaaaatta agatctctga tcaacagtta
      361 cagcgtaatg tatcagaatt atctgggggt caacaacaaa gggtagcatt ggctcgtgct
      421 ctggttcttg aacctaaaat tctttgtcta gatgaaccat tgtcaaacct tgacgcaaaa
      481 ttacgtgtag atttgagaaa agagttgaaa agacttcaaa aagagttagg tattactact
      541 ttatatgtta ctcatgatca agaggaagcc ttgactttat ctgatagaat tgcagtcttt
      601 aacaatggat acatcgaaca ggtcggtaca ccagtagaga tttatcataa ttctcaaact
      661 gaatttgtat gtgattttat tggagatatt aatgttttga ccgatgaaac agtccacgaa
      721 gtattattga aaaatacaag cgttttctta gaggataaaa aaggatacat tcgattagag
      781 aaagttcgat tcaatcgtga aactgaacaa gattttattc taaaagggac aattattgat
      841 gttgagtttt ctggagttac aattcactat acaataaaag tttctgaaag tcagattctt
      901 aatgtaacaa gtattgatag tcaggctgct attagatctg tcggagaaag tgtggaatta
      961 tttatcacac catcagacgt tctgcaattt taaggaggtg cagtatgcgt cataaattaa
     1021 atttaaaaga ttggcttatt cgtttagggt taatctggtt cttagtaaca tttattattt
     1081 atccaaactt tgatctagta gtgaatgtat ttgtaaaagg aggagaattt tcccttgatg
     1141 ctgtacatcg tgttctaaaa tctcagaggg cacttcagag tattatgaac agttttaagt
     1201 tagcattttc actcattatt acagttaatg tcgtaggtat tctttgtgtt ctatttacag
     1261 agtactttga tattaaaggt gctaaaattt taaaattagg ttatatgacc tctttaattt
     1321 atggaggagt ggttttagcg actggatata aatttgtcta tggtccttat ggattgatta
     1381 caaaattttt acaaaatgtt atcccttctt tagactctaa ctggtttatt gggtatggtg
     1441 cagtcttatt cattatgaca ttttcaggaa ctgctaatca tacattgttt ttaacaaata
     1501 caattcgaag cgttgactat cacactattg aggctgctcg aaatatggga gcaaaaccat
     1561 ttactgtttt ccgaaaagta gtgttaccaa ccttaattcc aactctattt gcacttacta
     1621 ttatggtttt tcttagtggt ttatctgcag tagcagcacc catgattgtt ggtggtaaag
     1681 aatttcaaac tataaatcca atgattatta catttgcagg gatggggaat tctcgtgatt
     1741 tagctgcctt acttgcaatt attttaggta ttgcaactac aattttgctt actatcatga
     1801 ataagataga aaaaggtgga aattatattt ctatctctaa gactaaagcg cctcttaaaa
     1861 aacaaaaaat tgcgtctaag ccttggaata tcattgctca cattgtagca tatggattgt
     1921 tcacagtttt catgcttcca ctaattttta tagtattata ctcatttaca gatccagttg
     1981 caattcaaac aggtaactta acattatcaa actttacttt agaaaattat cgcttattct
     2041 ttagtaatag tgcggcattc tctccattct tggtcagctt tatttattct attattgctg
     2101 cgacaacagc aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa cataaatctc
     2161 gttttgattt cttatttgaa tatggtgctc tacttccttg gttactacca agtacacttt
     2221 tagcagtaag tttattattt acttttaatc agccacaatt tcttgttttg aatcagattt
     2281 tggtaggtag tttggtaatt ctacttattg catatatagt tgtaaaaatc ccattttctt
     2341 atagaatggt acgtgctatt ttatttagtg ttgatgatga gatggaagat gcagcaagaa
     2401 gtatgggtgc ttcacctttt tatactatga tgaaggttat cattccattt attttaccgg
     2461 ttgttctctc tgttattgct ttaaacttta actctttatt aactgacttc gacttatctg
     2521 tattccttta ccatccccta gctcaaccat taggtattac gattcgatct gcaggtgatg
     2581 aaacagcaac atctaatgca caagctctgg tatttgttta tacaattgtt ctgatgatta
     2641 tttctggaac ggtattatac ttcacacaaa gaccggggcg taaagtaagg aaataatcat
     2701 gacagccact agtcttgggt tatcaaatat tgaaatagtt gtcaggattg ttttatcagt
     2761 agtcattggt agtataattg gtttagagag agggagcaaa tcccagcctg caggcatccg
     2821 aacttatagt attgtttgtc tagctgcatg tttgattatg atgacgaatg aatacgtatc
     2881 ttataaattt gggacaggag atcctacacg attaggagct caagttatat caggtgtggg
     2941 ttttctaggc gctggaacga ttcttattac agataagaag aaaattacag gtctgacaac
     3001 tgcagcaggc atttgggctt cggcaggaat tggattagct attggagtag gtttttatga
     3061 gggagctctt ttagtagcca tttctgtttg gggtgtgata tccatgttcc aaccactaaa
     3121 aaaatatctg caaaatcgtt ctaaaatgat tgaattgtat atagtagtta aatctacaga
     3181 agcctataat cgtgtattgg tttattgtgc agaaaatggt atcagaatga ccgattcaag
     3241 aacagcgttt ggagatgtta attctgacag aattgaatac tttgatgttc cggacaaaaa
     3301 aatagcgtca tttattactc tggaattatc aggtagattt gaacatcttc gactgatgga
     3361 agaaattgct aatattgtag gtgttattta tgtcgaagaa attagctgaa atttagtaaa
     3421 caaagggaca gatgtttttg tccttttgtt ttttagatgt tttaatcatc taatttttga
//
LOCUS       DAVLYR010000156         3418 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLYR010000156 DAVLYR010000000
VERSION     DAVLYR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.156"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            222..2351
                     /locus_tag="VYO63_001787"
     CDS             222..2351
                     /locus_tag="VYO63_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1672661.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            2338..2787
                     /locus_tag="VYO63_001788"
     CDS             2338..2787
                     /locus_tag="VYO63_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1672662.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            2845..3117
                     /locus_tag="VYO63_001789"
     CDS             2845..3117
                     /locus_tag="VYO63_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1672663.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         1000 a          642 c          770 g         1006 t
ORIGIN      
        1 ttgaaaagtc aatgtgttat tccaggtcag gaacatcttc cagaactggc tattatgtct
       61 gatccattaa atacttttga tatcatggct tggttaaaaa aaggtggcaa tgctctttaa
      121 gtgcaagtta ttgcgctgta gcatataaat ctatttccta catatttttt aaatgttcta
      181 cgagttaatt tgaaacgttt agcttgtggt ataatagatt tatggataaa aaatatgaaa
      241 aaatctctca ggatttggga gtgacgttaa agcaaattga taccgttcta agtttgacag
      301 ctgaaggggc gactattccc tttatcgcgc gttatcgcaa ggacatgact ggtagtctgg
      361 atgaggtggc gattaaggct attattgatt tggataaaag tctgacaaat ctcaatgacc
      421 gtaaggaagc tgtcttagct aagattcaag aacaaggtaa gttgaccaag gaattggaag
      481 aagctatctt agttgccgaa aaattagcag acgttgaaga actctatctt ccttataagg
      541 aaaagcgtcg taccaaggca accattgccc gtgaagctgg actctttcct cttgctcgtt
      601 tgattttgca gaatatagtt gacttagaga aagaagctga aaagttcgtc tgtgaaggat
      661 ttgcgactgg caaggaagcc ttgaccggtg cagttgatat tttggtcgaa gccttatcgg
      721 aagatgtgac cttgcgttct atgacttatc aggaagtgct gagacactct aaactcactt
      781 ctcaagccaa ggatgaaagt cttgatgaaa agcaggtttt tcagatttat tatgattttt
      841 cagagacagt tggaactatg caaggctatc gtaccttggc tctcaatcgt ggggagaaac
      901 ttggtgtctt gaagatcggt tttgaacatg cgacggaccg tattcttgcc ttctttgcta
      961 ctcgtttcaa ggtgaaaaat gcttatattg atgaagttgt tcagcaatcc gttaagaaaa
     1021 aggtcttgcc tgctattgag cgtcgtattc ggacagaatt aactgagaaa gctgaagagg
     1081 gagctatcca acttttttct gacaatctgc gcaatctcct cttggttgct ccactgaaag
     1141 ggcgcgtggt tcttgggttt gacccagcct ttcgtacagg tgccaagcta gctgtcgtgg
     1201 atgcaacagg aaaaatgctg acaactcagg ttatttatcc tgttaaacca gcatcagctc
     1261 gtcaaatcga agaagccaag aaagatttag cagatttaat tggtcaatac ggtgtagaga
     1321 ttattgccat tggaaatgga acggccagtc gtgaaagtga agcttttgta gcggaagttc
     1381 tgaaagattt ccctgaagtc agctatgtta tcgttaatga aagtggtgct tctgtctatt
     1441 ctgccagcga acttgctcgt caggagtttc cagacttgac cgttgaaaaa cgctctgcca
     1501 tttctatcgc ccgtcgtttg caagatcctc ttgcggaatt ggtcaaaatc gatcctaagt
     1561 caattggtgt cggtcaatac caacacgatg tcagtcagaa gaaactatct gagagtctgg
     1621 actttgttgt cgatacagtg gttaaccaag ttggtgtcaa tgtcaataca gctagcccag
     1681 ctcttctttc acacgtagct ggactcaata aaaccatttc tgaaaatatc gtcaaatacc
     1741 gtgaggaaga gggaaaaatc acttcacgct cccaaatcaa gaaagttcct cgtttggggg
     1801 ccaaggcttt tgaacaggct gctggtttcc tccgtatccc tgaaagtagc aatatccttg
     1861 ataatacagg agttcaccca gaaaattacg ctgccgttaa ggaactcttc aaatgcttgg
     1921 acattaagga tctgaatgaa gaggcccaaa gtaaactcaa gtcgctttca gtcaaggaaa
     1981 tggcgcaaga gttagacctt ggtccagaaa ctcttaaaga tatcattgca gatcttctca
     2041 aaccaggtcg agatttccgt gattcctttg atgctccagt tctccgtcaa gatgtcctag
     2101 atatcaagga cttagtggtt ggacaaaagc tagaaggagt ggtgcgtaat gttgttgatt
     2161 tcggtgcttt tgttgatatc ggaattcatg aggacggtct catccatatt tcccatatga
     2221 gtcgcaagtt tatcaaacac cctagtcaag tggtggcagt tggagatttg gtgagcgttt
     2281 gggttaagaa aattgatact gaacgtgaaa aagttaatct gtctctccta gctccaaatg
     2341 aaactgactg attacgtcaa gcaggtttca ctagaagact tcggcagacc ttttatccat
     2401 catgtccagt ggaataggcg tctacgttcg acaggtgggc gatttttccc caaagatggg
     2461 catttggatt ttaatcccaa ggtttatcag gaactggggt tggacgtttt taggaaaatt
     2521 gtccgacatg aactctgtca ttatcacctc tattttcaag ggaaaggcta tcaacacaag
     2581 gatcgggatt ttaaggaact gttgaaagca gtggatggat tacggtttgt accatccttg
     2641 cccaatagta actccaagcc aatcaagctc tatcgttgcc aatcctgcca gcaaacttat
     2701 cagcgtaagc gtaggattga tacccaacgc tatcgctgtg gactttgtcg aggaaagttg
     2761 ctattagtaa atcagcctga ggactgatga aagccgagcc tatccgtgat atactagtct
     2821 aacctagcag aaagaggaaa tatcatgaac agtaagtttt ataaaatgag acgaaatcgc
     2881 atgatttcag gagttttagc aggtctgtca gacaagtgga actttgatgt tacccttgta
     2941 cgttttctgt ttgccatttt tacagtcgca aactttggac ttggggtgat tatctatatc
     3001 atcctagctt ctatcatgcc aaccaaggaa gaaatcgaag cagaaatgta cggaacaggc
     3061 ccacgtaaac gcaaagaagc tcaagccatt gacgataatg aaggctggtt ttggtgaggt
     3121 tttacaaaag atttggaata cgcaaaaagc tttaaatcaa tagtctaaac tgcttgattt
     3181 aaagcttttt tacttttata ctcaataaaa atcaaagtgc aaactaggaa gccagccgca
     3241 ggctgtaatt tagtcggcaa tgtgaagttg atatagtttg cactttgatt tttgaatagt
     3301 attaataata tttcgccagc ttcatccaca aactatagtg gattggaact agaataggac
     3361 acctatactt ctaaaaaatt gttagaaatc gatttgactg tcctgatcga tttgtcct
//
LOCUS       DAVLYR010000157         3416 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLYR010000157 DAVLYR010000000
VERSION     DAVLYR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.157"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            192..269
                     /locus_tag="VYO63_001790"
                     /pseudo
     CDS             192..269
                     /locus_tag="VYO63_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(507..977)
                     /locus_tag="VYO63_001791"
     CDS             complement(507..977)
                     /locus_tag="VYO63_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1672664.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(994..3267)
                     /locus_tag="VYO63_001792"
     CDS             complement(994..3267)
                     /locus_tag="VYO63_001792"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1672665.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHINKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         1029 a          600 c          757 g         1030 t
ORIGIN      
        1 taggaagaat gtcatcgttt tcctagaata cagtatcagt tgttaagtgg ttgataaatt
       61 tcaaagtaga tacttgtacc acgatgtttg ttgatcgagt tattaacaaa agagctactt
      121 tgattttaaa gaaatagaaa acaaaaagcc gagcaagaat tcaattgcag gagaaaatga
      181 aataatactc aatgaaaatc aaagagcaaa ctaggaaact agctgcaggc tgctcaaaac
      241 actgttttga ggttgcagat aagactgacg aagtcagctc aaaacatggt tttgaggttg
      301 tagatgaaac tgacgaagtc agtaaccata cctacggcaa ggcgacgttg acgcggattg
      361 aagagatttt cgaagagtat aaatcttcct aggataaagc aaaacgcata gtatcaaggg
      421 ttttcaacac ttgatactat gcgttttctg atgttaaaga ctttctacca gtttttttaa
      481 gcataattgt tagttgtagt catttattat tcttcaaaga aaaatggtgg ggcgaatttt
      541 ttcagttctt caaagcactt ttgagcagta tctgcatctt cacagatgat aagacagaca
      601 tcattaccac aaagggtagc gatagcgtca gggaagctca aagtatcaat gatagaacca
      661 aaggattgag ccagtccagg aagggttttt agtaggactt ggtgttgaac tgggcgcatc
      721 cagacaaggg cgtcttccat gtagagttcg agacgttttt cccattttga gatggaacca
      781 ttgttaagaa cataataagc gctatcttct tcgcggactt ttgataggtt catatttttg
      841 atgtcgcgtg agagggttgc ctgggttact tgaatgtcgt tcttagcaag aagggcttgc
      901 aactcagcct gtgtatgaat cttgtttttt gtgataagag cgcgtataag ttggtggcgg
      961 tgttctgatt tattcataat aatgtaactc cttttagcaa ggtaaggtaa gcatggactg
     1021 agcgaggtcg acagtcaagt ggtagtctgt attgtcacgg atggtgattt caaagtcagt
     1081 agtatagagg actaaacgga gagtgtctcc ttcttttagc ttgtaaatag ttggctgcag
     1141 ttcaaattga acatccatcc attcatctgc agtaatatcc tctactaaca gtaaatcatt
     1201 tctattttgt aaattaaggt aaccttttgt cacgactcgt tgtgcctctg gtctaaatgg
     1261 caattcacag agattttcca acatgtgata gcgaccgttg tcaatggttc tagcacttaa
     1321 aatagctgga taaggttgta ggtatttctt ttgcccaaat tctagcagtt gggcagataa
     1381 gagccccttg tttgtactgg atttgatacg aagattgagc tgagcgcgac cgtttaggtg
     1441 gagatcttta gtcacaggaa ggttaatagt aatctgattg gctttccctt gatagagctc
     1501 tgtattgaag gtttggtatg tcttaccata gcgctcaaaa tccttatctg ggtactggtt
     1561 ttgaatagct tgctcttctt gaccaagtga gaaggtttca cagttttctt gcccaccgaa
     1621 gttatcaagt gataaccaag tctgtggagc tgtattgtcc tgccagataa cagtaggaag
     1681 ttgaaagtct gtttcctgtc ctagtaattt cttggtcaat aaggcattta tggactcacg
     1741 gaagtcaatt gattgccaat tgttcatgta aacatgggca ccattatgga aaaagagatg
     1801 cttgtttata tgagtaggaa gagcatggaa catctggtaa acatgaagtg gtttgacatt
     1861 ccaatcctga gaaccatgag taaagacaac ctctgccttt actttatggg cattgagcag
     1921 ataattgcgg tcatgccaaa actgattgta gtccccagtt tttcggtcta gctgagcttt
     1981 cactttttct aagtcagctt ggtgagcttc attgccacgg atatagtcgc cagctaagag
     2041 attacgagaa taggttaact cagcaaggga gtcaaagtcc tcacctggat aaccacctgg
     2101 gctagtcacc agaccgtttt cacggtagta gttgtaccat gatgaaattc ctgcctcggc
     2161 aatgataact tctaaaccat cgactcctgt agtcgcaaga ccattggaca tggtacctag
     2221 ataggaaagt cctgttgtag caacttttcc gtttgaccaa tcagccttga cttgacgctg
     2281 gcgcgtgtga tcagtaaagg cacggcaacg gccgttaagc caatcgatga catttttata
     2341 agcctcgatt tgctggtagt ctccattagt catgaaacct gtcgagtctt tggtaccaac
     2401 acctgagaca tagagattgg caaagcctct cggaaggaag tagtcgttta gtgtatagct
     2461 agagttgatg tgagttagct tttcctcagc ctctgctata agctcagctt taccttgggg
     2521 ttggacgaga tttagttgag gtttctctag ctcaatcttg tgaggaagct taacctcaag
     2581 ctcgccctcc atcttgtaga gagccttgtc actagccttg tcattggttc cctgatgata
     2641 agggctggct gtcatgatgg cagggatttt tccatcaaaa cgagggcgaa taatgctaac
     2701 ctttactagg tctgatagcc ctttttggtc agtatcgaca cgagactcaa cgtaaacgac
     2761 ttcacgaatg acatcctggt tagaaaaagt agccaaactc ttgccgttaa agtagtggta
     2821 gtcattatcc tccggaataa gaccatcact aacaagttgg tcgataagag tatttccttt
     2881 tttggtgcga gtattgagta actgatagag attttcaatc aagtcaccat atataatggg
     2941 aaatccagtt tctttacgaa aaacgtcact atcttcgaag tcaaccaaat aagaaaagcc
     3001 taaaagttga aaagcaacag tataaaaaat atctgctgtc agttcatctt ctgattgaaa
     3061 aaatgtcagc aggtctgttt ttttatcagc tgctaggata gaaagtgggt agttggtgtc
     3121 ttgataagtg aaaaagaaac gacgtaaaaa ggtttcaagt gagtctttgt gattggctgt
     3181 attttgtaaa tcaaagccac atttttttag ttcagataag acattttctt ttggaaaatt
     3241 gatataacta tattgattaa aacgcataga acctccatat agaatgacag ttaaggttat
     3301 tatatcaaaa aaaaagcaga aagggaattg ttaacttcaa aaggaaataa tccaataaaa
     3361 atgaataaag tactaaattc aatatagaga acagagtaac aataagaata aataga
//
LOCUS       DAVLYR010000158         3331 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLYR010000158 DAVLYR010000000
VERSION     DAVLYR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.158"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            9..334
                     /locus_tag="VYO63_001793"
                     /pseudo
     CDS             9..334
                     /locus_tag="VYO63_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            407..853
                     /locus_tag="VYO63_001794"
     CDS             407..853
                     /locus_tag="VYO63_001794"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672666.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERISIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            1193..1447
                     /locus_tag="VYO63_001795"
     CDS             1193..1447
                     /locus_tag="VYO63_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672667.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            1558..2664
                     /locus_tag="VYO63_001796"
     CDS             1558..2664
                     /locus_tag="VYO63_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1672668.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            2622..3230
                     /locus_tag="VYO63_001797"
     CDS             2622..3230
                     /locus_tag="VYO63_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1672669.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
BASE COUNT         1234 a          585 c          690 g          822 t
ORIGIN      
        1 ataaagctat ggcatatagt actgatttta aacagcgagc attagattac atcaaagagg
       61 ggcacagcca tgtcgaggca gccaagtttt ttggtgttgg cgtcagaact ctcttcacgt
      121 gggaaaagaa agacgtgaac aagaacacat agagaggaaa aagcgagtcg tcaaaaaccg
      181 aaagattcct ttagaggaat tgaaagcctt tgtagaggct catccagatg cttttttacg
      241 ggaaattgcg gcacattttg attgtgctgt tccttcagta tgggcagctt taaagcagat
      301 taaggtcact ttaaaaaaag atgacgagct ttaaggaaca agacccagaa aagtagcctt
      361 atttcttaag aattttaata gtttaaagca cctagcacct gtttatattg atgaaacagg
      421 aatcgaccgc tatctctatc gtccttatgc aggggctcct agaggggaga aagtctatga
      481 aaagattagc ggacgtcgtt ttgagcgaat ttcaattgtt gcaggacaag tagacggaga
      541 gtttatagct cccatgattt acaagaaaag catgacaagc gatttctttg tggagtggtt
      601 caaaacgcaa ctcctacctg ctttgaagac acctcatgtt attgtcatgg gcaatgctgg
      661 ttttcatccc aagaacattt tggatgaact ctgcatccaa gataaacact ttttcttacc
      721 tctaccacct tattcaccgg atttgaatcc tattgagcaa gcttgggcta tcttgaaaaa
      781 gaaagtgacg gatttattaa gggaagttcc aactattttt gaatgtttgg aatgcttttt
      841 taaaactaga tgactataac ggttctaaag gaacctatcg agtagtcatt aaaactaagg
      901 atactgctgg ttaagagaag acggtataca atcaaaccat tcaccgtgta gccgaaatcg
      961 ttcagaatga agacttgtat aaaaaaggtt tgaatgttga acttgcgcac caacaaatta
     1021 agggattttt tgaagcagag tttaaaaatc gtattaatgg agttcttaat actaaaataa
     1081 aaaatagtac attaaatcgt gtaaataaaa aaactataca ccagagcaac aaaaactcca
     1141 tgatcaattt gaagcagaag caacggaaga tgctaaaaaa caaggcgata ttgtgttgaa
     1201 tgttgaccag gatttcatga gcatatctaa gtctaataaa agtggttcag actggaagaa
     1261 aactttcaca gtgaggacaa ccaataggct agcaaatgac ttgaataatg tcttgaaaca
     1321 ggctgataaa gatactccta ataccccaac ttggctaaac tcagctgctt ctaaagctaa
     1381 agatgatgac agagcatata aactactgaa gactcttata ccaggagaaa attacctatc
     1441 atgttaagga taatcagcta gaagtagaaa cagataaata cacatatact gccgctagaa
     1501 atggtagtaa ggaagttggt attcaagagt cagatatagc agcaactcta agtgccgatg
     1561 aatataattc taatcgccaa acttttgaga gagaatacaa atacaaaagc aaatgccctt
     1621 aataatggtt gggctagatc tggttctgaa gagttcaaaa agttctccca ctttgtaggg
     1681 gtagacaaag ggattgtgcg aacgaatgta ctgactggta aaaaactatc tgataagatt
     1741 aggaaagaag tgggctctgg agatagcaaa ctaggaaaag gcggctattt ctctactggg
     1801 gatgttctat taggaaaaga tgttgtttct tataccgtac aagtattttc agagaataat
     1861 gaaagagtag gagtaaacac tcaaagtcac cgtgttcagt ataatctccc aattctagct
     1921 gacttttcag tcatccaaga tactgtggaa ccatcacgaa ccgttgttga aaaaatcatt
     1981 ccaaaactaa atattcccga agaagagaaa gggaaaataa ccgaagaaat caagaaaaag
     2041 aaaaaaacct cagaattggc agaactaatc tcagaaaatg tgaaagttcg ctatgttgat
     2101 gaacaagggc gtttgctatc attgaaaaat gatactggaa ttggagaaaa agaaagtgac
     2161 ggaacctaca ttaccaataa aaaacaactg attggtacca gctacaatgt cacagataaa
     2221 aaactcagta gcatgactac tactgacgga aaatattata cttttaaaga agcagataca
     2281 aattctgcaa gtttaactgg gaatattgta agcgaaggta gaacagtgac cttagtttat
     2341 agagaaagcg aagcgccaac cactgctaca gtaacagcca attactataa agaaggtagg
     2401 caagagaagt tggtagagtc tgttataaaa gctgatttag cgataggttc tgagtatacc
     2461 acagaatcaa aaactattga agggaaaaca acaactgagg acaaagaaga ccgagttatc
     2521 acaaggaaaa caacatacac cttggtagca actcctgcaa atgcgtacca gaagacggtg
     2581 caacagttga ctattactac cgtgagaatg ttgaggaaac agtggttccc aaaacagcaa
     2641 cctctactga gacgaagact ataacgcgta tcattcatta cgttgataaa gttacgaacc
     2701 aaaatgtaaa agaagatgtt gttcaacctg taaccttaag ccgtacaaaa actgagaaca
     2761 aggtcacggg agttgtaacc tacggtgaat ggacaacagg aaactgggac gaggttatat
     2821 ctggtaagat tgacaagtac aaagatccag atattccaac agttgaatca caagaagtta
     2881 cgtcagactc tagtgataaa gaaataacgg taaggtatga ccgtttatca acaccagaaa
     2941 aaccaatccc acaaccaaat ccagagcatc caagtgttcc gacaccaaac ccagaactac
     3001 caaatcaaga gactccaaca ccagataaac caactccaga accaggtact ccaaaaactg
     3061 aaactccagt gaatccagac ccagaagttc cgacttatga gacaggtaag agagaggaat
     3121 tgccaaacac aggtacagaa gctaatgcta ccttggctag tgctggtatc atgaccttgt
     3181 tagctggtct aggattagga tttttcaaga aaaaagaaga tgaaaaataa tagattttag
     3241 aatctaggaa ccaggaaaag ctcacagatg tgggcttttt tcctggtttt gagaacgagg
     3301 tctttcgtaa agaataaaaa cgcttacaag t
//
LOCUS       DAVLYR010000159         3305 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLYR010000159 DAVLYR010000000
VERSION     DAVLYR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.159"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            72..524
                     /locus_tag="VYO63_001798"
                     /pseudo
     CDS             72..524
                     /locus_tag="VYO63_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            629..977
                     /locus_tag="VYO63_001799"
                     /pseudo
     CDS             629..977
                     /locus_tag="VYO63_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1248..1469)
                     /locus_tag="VYO63_001800"
     CDS             complement(1248..1469)
                     /locus_tag="VYO63_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672670.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1434..2063
                     /locus_tag="VYO63_001801"
     CDS             1434..2063
                     /locus_tag="VYO63_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672671.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2099..>3305)
                     /gene="pepT"
                     /locus_tag="VYO63_001802"
     CDS             complement(2099..>3305)
                     /gene="pepT"
                     /locus_tag="VYO63_001802"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1672672.1"
                     /translation="LDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGL
                     QNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDGGVIELG
                     NSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAHPEIKHC
                     EIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRN
                     VHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEVRASYII
                     RDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTPITIAKA
                     VMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDT
                     IIGIVAYKG"
BASE COUNT         1093 a          642 c          642 g          928 t
ORIGIN      
        1 ttgttatcct ttccttaagt ctgtgtgttt atgctctgaa ccaacatagc taacaggcca
       61 atacagataa aaatcgtgtt tcatatgtaa acagtaataa agacactaag aagactgaaa
      121 atttgactcc agactaggtt agtcaaaaag aaggaattca agcaaaacaa attgttatta
      181 agattacaga tcaaggttat gtaacttctc atggcgatca ttatcactac tataatggta
      241 aagttccttt tgatgcgctc tttagtgaag agccattaat gaaagccccc aactatcagc
      301 cgaaagatca agatattgtc agttaggtta agggaggata tattattaag gtagatggaa
      361 agtattatgt ttaccttaaa gatcaagctc atgcagaaaa tgtacgaaca aaagatgaaa
      421 tcaatcgcca aaaacaagaa catggtaaag acgataaagg agctagtgca gaagtatctg
      481 tagcaaaatt acagggacgt tatactaccg atgatggtta tatatagtga gttgaattta
      541 gaatagcaca tcatagtttc taaatcattg ttagaaatta ctttaaaatc cccttatcaa
      601 tttgctcata ttctatttca atctactaat agtaccacat ggtggtcact atcatttcat
      661 tcctgagagt gacttgtcgg caggtgaatt agcagccaca tatgtcttta accctaatga
      721 tattgtcaga gatataggtg atgcttatat agtaagacat ggggaacact atcactatat
      781 tcctaagtca tctcttaaca atcccccatc acactctaac acggaagagg taggaagttc
      841 ttctagttct gtattaagta atccgtcact acatgtccac catgaggaag aagatggtca
      901 tggtttttga tgccaatcga attataagtg aagattcaga aggttttgtg attcctcatg
      961 gcgaccacaa tcactacatc aaggttcaaa ctaagggata tgaagcggct ttgaaaaaca
     1021 agattccatc cctgcaatcc aactatcaac ctggaacttt tgatgagaag gcagtcttgg
     1081 caaaagtaga tcaacttcta gctgatagca gaagcatcta caaagacaga ttgagttagc
     1141 tttaggtcag tttactgaaa gtttgaaaaa aataaaagtt agttgattga ttacttctct
     1201 aaattttggg cacactaaaa agaggtcttg acacctaaac tttaaaatta aagcatattg
     1261 aatccgtgct gctgtgcgaa gcgaatagcg tcttggacga acattgtctt atctggattt
     1321 cttatttctg taaggccttg taatcttgga ttataactct cctgttttag gagtacatgg
     1381 tagatagcaa ccaataatct gcgacagata gcaaggaaga agtagaaagt cttttgaaca
     1441 aggcaaatca actcttggaa caaatccatg aagaaggaat cagacaatcc ttggcagaag
     1501 aagtagaaaa tctaaaagct gccacaaaca aggctgatgc agacttggat gaagtaaaca
     1561 gtcaggtaaa agatgttttg actcgtatcg ctagcgccct tcaacaagaa aaggaaaatg
     1621 ctgagcaaga ttctcagaca cttgtactct atcaaaaact ctacgatatt ctcatgtcgc
     1681 ttcactctta tttagaaagt aataatggat ctgatgcgga ctttgataag gtggatgcat
     1741 tattagatca actttctgct aatagtaaag acaaaacagc tttacttgaa tcgacaaaag
     1801 ctattctagt tttgaatcat caaatccagt caaaagttag cgcaagtgaa gaaacaagtc
     1861 cagcaagaaa tgctgaagca aatagtgata acaccagtgc tgagaaccaa ccaaacgcaa
     1921 ctgcagaatc taacattgaa actgctagcg acgaaaacaa accaagcaat acaagagatt
     1981 ctaaacctgc tgaatcaact tcagaaaatg aaacaacaga atcttctaca actactggaa
     2041 atcaagaaaa accagtagaa taaaaagaaa ggcgaagtag ctgagccacc tcgtcttttt
     2101 agcctttata agctacgatg ccaatgatgg tatcaactgc acgttccata gtctgaaggc
     2161 taacgtattc aaaacgtccg tgcatatttt cgccacctgc aaagatattc ggagttggga
     2221 ttcccataaa ggaaatctta gagccgtctg ttccaccccg gattggttcg ataataggcg
     2281 tgatacctag atcttccata acggctttag caatggtaat tggagtcata tctttttcaa
     2341 tgacttcttt catattgtag tactggtctg tcaagttgag agtgacacgg tcgctcccaa
     2401 gttcttcatt catcttatca gcgatagatt gcatggatgc tttacgcgct tcaaaggcat
     2461 ctttttcaaa atcacgaatg atgtagcttg cacgcacctc ctcaacacta cctgtcacat
     2521 ccattagatg gtaaaaacct tggtaacctt cagttaactc aggtcggtca ttttctggaa
     2581 gttgattatg aaaatcaatt gctagctgaa gggcattgac catctgccct ttggcagtac
     2641 cagggtggac attacggcct tggaaatgca attcagcacc agcggctgag aaagtctcgt
     2701 actgaagttc acctagtgga ccaccatcaa cagtgtaggc aaaatccaca tcaaaatctt
     2761 ctgcatcaaa tttattggca ccaacaccga tttcttcatc tggaccaaaa ccaacacgaa
     2821 tctcacagtg cttaatttca ggatgagcag ttagatattc aatggctgtc ataatttcag
     2881 caattcctga cttgtcatca gcacctagca aggttgttcc atctgttgtg atgagtgttt
     2941 gtcctggata tttttcaaga ctcttgaagt cggctggatc gagtttgaaa ccagaattcc
     3001 ctagttcaat cacaccacca tcgtagtttt caattacctg tggattgact ccttcagcat
     3061 taaaatcagc agtatccatg tgcgatataa aaccaatctt acgtgttaaa gacggatcgt
     3121 tggctggcaa ggttccaata gcaaaaccat tcggtagata gtaaacattt tgcagtccaa
     3181 cacgtttcat ttcaggaatt aggacatttg ttgcgaagtc aacctgactc tgtgtacttg
     3241 gagtagtagt agagtgttca tcagagcgcg tgttgacctt aacgtaggtt aagaagcggt
     3301 ccaag
//
LOCUS       DAVLYR010000160         3270 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLYR010000160 DAVLYR010000000
VERSION     DAVLYR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.160"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..206
                     /locus_tag="VYO63_001803"
     CDS             <1..206
                     /locus_tag="VYO63_001803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672673.1"
                     /translation="AGCSKHCFEVVDETDEVSSKHCFEVVDETDEVSSKHCFEVVDET
                     DEVSNHTYGKAKLTRFEEIFEEY"
     gene            complement(203..418)
                     /locus_tag="VYO63_001804"
     CDS             complement(203..418)
                     /locus_tag="VYO63_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1672674.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            516..836
                     /locus_tag="VYO63_001805"
     CDS             516..836
                     /locus_tag="VYO63_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672675.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(867..1205)
                     /locus_tag="VYO63_001806"
     CDS             complement(867..1205)
                     /locus_tag="VYO63_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672676.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(1217..1936)
                     /gene="trmD"
                     /locus_tag="VYO63_001807"
     CDS             complement(1217..1936)
                     /gene="trmD"
                     /locus_tag="VYO63_001807"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1672677.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(1926..2444)
                     /gene="rimM"
                     /locus_tag="VYO63_001808"
     CDS             complement(1926..2444)
                     /gene="rimM"
                     /locus_tag="VYO63_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1672678.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(2475..3263)
                     /locus_tag="VYO63_001809"
     CDS             complement(2475..3263)
                     /locus_tag="VYO63_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1672679.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
BASE COUNT          960 a          708 c          557 g         1045 t
ORIGIN      
        1 ccgcaggctg ctcaaaacac tgttttgagg ttgtggatga aactgacgaa gtcagctcaa
       61 aacattgttt tgaggttgtg gatgaaactg acgaagtcag ctcaaaacat tgttttgagg
      121 ttgtggatga aactgacgaa gtcagtaacc atacatacgg caaggcaaag ctgacgcggt
      181 ttgaagagat tttcgaagag tattagttgc ctttaaaggc atccaccatc gtttgaaatt
      241 cttcatttga gagagtaatc cctttgccca ttttagtatg gtctggactc caagcacgaa
      301 tatcaaactt tgcaggggca ccattaaagc tcacacggtt aatttccttg gtccaacctt
      361 tttcgttttc agaaagagtc aacaagtgct cttcgatttc aaatgtaaat tctgccattt
      421 tcttctcctt ttttagtttc attagtttat tcgtaaaatc ttgtagattt taggaaaatt
      481 ttatataata ttgatataaa agaagggagg ccaatatgag acataaattc cagcaagttc
      541 taaataaaat acatgatttt ttaaatggat atgaccaacc tgaccagact gaaaccaact
      601 cccttacagc cactattgaa gaggctatcc agaaacaaac cgctgttcac cttatcttat
      661 ctgagacaag ctttacaggt gacatcatca aatatgatca gcaaggccag caaattatcg
      721 tgaaaaattt ttccaaaaat gtgagccgga ttatccgtat aagcgatatt caacgcctgc
      781 gatttgtccc ctcaactgtc caaacagccc aaaaaaatag atttaagaaa gagtgagatg
      841 tagttgcttc atcccactct tttttcttag cgaatttgtt caaaatgtaa atgaactgcg
      901 atatgatctc cataaccact tctttccaag tcacgttgta aacgatagga aatgtagtgt
      961 tctgcaatgg taatgtaacc tgcgcccaat aaacgatgtt caaccataga ttgaatcata
     1021 ctgatagtcg cacgttccac cttggcttct tgtaaatcca aaactacctt cttagtgact
     1081 tgagcaagat tttgacgcaa atcatctgtc aaaacataaa cagtttgggc tgccttcaag
     1141 atggcttggt aaatcttatc tggattaaat tcagcaattt cgccattacg tttgattact
     1201 tgcataggtt tctcctttat tctttgtttt ctttgatttc tgccagcatt ttttcttctt
     1261 ctactgtcag ttgataatgt tcaagtaaat ccggtctgcg ctcgtaggtt ttctttaaac
     1321 tctcgtacaa tcgccactga cgaatctttt catggtggcc actcatcaat acatctggca
     1381 cgaccatgcc tcgataatca taaggacgtg tgtactgagg atattctaaa agacctgaag
     1441 aaaaactatc atcttggtgg ctagactcct tgccaatcac ttctggaatc aggcgaactg
     1501 tagcatcaat catggtcata gctgccaatt ctccaccagt gaggacatag tcgcctaggg
     1561 aaatctcatc tgttaccaag gtcttaatgc gctcatcata accctcatag tgcccacaga
     1621 taaagattag ctcttcctct tgagccaaat cttcagcata agcctgatca aactgctttc
     1681 cagcaggatc gaggagaata acgcgcggat ttttcttttc aatagcatca aaggaatcga
     1741 aaataggttg tactctgagc aacatgccct gaccgcctcc gtagggctca tcatctacat
     1801 gacgggcctt ttcagcattt tctcgaaaat tatgatactg gatatccaag agcccttttt
     1861 ctcgagcctt tccaacgatt gagtgctcca gtggagaaaa catctctgga aagagggtta
     1921 aaatatcaat cttcatcgtc taacccttct aagatttcca catcgacccg tttatttgga
     1981 atatcaacat tgagaaccac tggtgggata taaggtaaaa gcaaatcacg tttgcctttt
     2041 cgtttgacca cccagacatc attagcacct ggttgcagga tttccttgat ggttccaacc
     2101 aagctatcac cctcatagac ttccaaaccg ataatctcgt gatagtaaaa ttcaccatcg
     2161 tctaggtcat tcaaatcttc ctcagcgacc ttgagactgt atcccttgta cttttcgata
     2221 gtattgatat ggtacatatc tttgaattta ataatgtcaa agttcttctg tttacggtgg
     2281 ctagcgatgg tcactgtttg gacaaactga tctttttcat caaacaaagc cagctcagct
     2341 ccttttttaa accgttcttc tgcaaaatcc gtcacagaca agactcgcat ctcaccctgt
     2401 aatccctgcg tattaacgat tttcccaaca ttaaagtagt tcatcttgtc tcctgtaatc
     2461 tccttttttc catcttattc taacaattct cgaataatag ccgcaatttt ttccgattct
     2521 gaccattgta aataatggtg attccctcct aaaatgagtt tagtattgga agtccaatat
     2581 tctgattctc tgtactcttt ttctctataa ggctgacaaa aaacaaatac aggaatatga
     2641 gcttctatag atacatcctc aaaatcttcc tcagtaatct ctccagatat ctgaaattct
     2701 ggatcttgat tttccaactc taagcctttt tcttgcatta attcccagat ttttttattc
     2761 gtttcaggac taaatgttgc ttgagttaag ttcttaaaat aaagttcagg accacactcg
     2821 tcaatcagcc tcatctgctc ttccatttct ggataaggat tttctgaaaa atcagcaaac
     2881 atgacttttt tagttgtcgg ttcaattgct actaaagtct gacgcttaat tggtttctcg
     2941 agtaatttgc aagctaaaat tccactccaa ctatgtgcac aaagtatata ttcagaaatt
     3001 cctaattctt caagtacttc ataaaccgca tctgcaagat tatctagatt ttttccagct
     3061 tggtcatgaa tcggactcct acctgtgttc ggaaaatcaa ttgtcaaata accaattgta
     3121 ggaggaggtt tttcaagtat aagtgaaaaa ttttcataac ttggtagcaa acctgctccg
     3181 tttagacaaa ctagcacttt cttttgcttt tgataagtaa cagagaggct accaatttct
     3241 gtagatactt caaacctctt cataaagaaa
//
LOCUS       DAVLYR010000161         3262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLYR010000161 DAVLYR010000000
VERSION     DAVLYR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.161"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            301..435
                     /locus_tag="VYO63_001810"
                     /pseudo
     CDS             301..435
                     /locus_tag="VYO63_001810"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(673..2105)
                     /locus_tag="VYO63_001811"
                     /pseudo
     CDS             complement(673..2105)
                     /locus_tag="VYO63_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(2361..>3262)
                     /locus_tag="VYO63_001812"
     CDS             complement(2361..>3262)
                     /locus_tag="VYO63_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HEV1672680.1"
                     /translation="KISDEKLYDVNFFQDYFKNKEVGVSEGIENLQAQYRAFQEERDK
                     EKVSTEEIEEAFETFKEKRDAVHEFEVKLTEHQIEKLVDEGIYIKVSFGINQSGLIFI
                     PNYQLDIMEEENQKKYKVYIRETTSYFVYNKEHSDKNQYIKGRTLIRQLTNDSRVIPY
                     RRPTVERLQEKISEISLLIELTETDKKYQDIKDNLVSEIAELDIKLTQTNEKIATLNK
                     MAEVLINSKSEGSGSQKLARHEFSKLNMTESTTLEQVNEELLKLQQEFGNVLDEYEKT
                     IRKLGQLFKVFDECINKEIMNEI"
BASE COUNT          977 a          636 c          537 g         1112 t
ORIGIN      
        1 tttgtctcta tgctaaagga atccgctata aatacaagta ctatagccgt cggcattcct
       61 cattaacacg acccaatttg attaatcagt gttttcaaac aaaaggtaaa aatgaaatct
      121 ggctggggga tttaacttat attccgacaa aagcaggtac tctttattta tcattatata
      181 tatgtttata gcagaaaaat tgttggttag gcaatgggaa atcgaatgca agataagttg
      241 gtaacgaatg ttttcaatca agcttatcat agagaaaatc tcaaataaga actagtcggc
      301 cataccgatc aaggatctca atatactgga gctagatttc aagaactcct taaacagaaa
      361 agatgcaagt ctagcctgag tcgaaaaggc aatccatatg ataatgcacc catggaatcc
      421 ttttacaaaa cataaaaaga gaacttgtca atgatgccaa tttcgcaact attgaacgaa
      481 atctttaaat atattgaaat ctattacaat cctaaatatc tccattcggc attagggtac
      541 caataactaa tagagtttga aaaaaatagt cactaatcat cgagtccact gaaaaagtaa
      601 gctctacagt tcaactaaaa tgaaaaaaga gggatctatc tcctgcttgg atagattcct
      661 ctttgctttt ccttattttt ggtttattag agttataatc ctcttcatat taaccacaaa
      721 tatggttgtg gcggcttgta actccatgtt taaaagacct ggtgctctag ctacattaag
      781 accatgtctg ttttcaagtt ccgcattttt ggcttctatc ttgtagcgat gtttggcctt
      841 ttctttaaaa tagggcgctt cctgaaacct cttctggaaa atatgttcat cgctcttaag
      901 gggtatcaaa taagtcttag acttagcccc ttctttataa cagcccactt ttgaagggca
      961 tatttgacag tgcttgatgt caaaatagtg ggtttcctga gccgtccttc ttcctgtctt
     1021 ttccttcttt agtgccatat gcccttctgg acaaacaaag agacctgcgt ccttgttata
     1081 gaaaaactca tcttccttcc tacgaaagcc ttttgaaata gttggatatg gcctagcgac
     1141 tcaatgcaca cgttctttct tggtaaattg catattatcc ttcccagagt aggccctatc
     1201 tccaacaata gcttcaatcg tcacgccatt atccttagat ttgtgataga gttcttctaa
     1261 aacaggacca tcactttttt cacccgatgt caccacacag gcagtaataa tccgttcatc
     1321 ggtcatggca aaatgctctt tgtacccgta aaaggaacta tcggctgact tatgtccaat
     1381 tcttgcttct tctttgacag aggattccaa gtgctctaag tcatcctcta cagcttcttt
     1441 aaggtaattg aattttgtga gatagctggt attccagtca attcgtcatg cttctcaagg
     1501 actgatatta cttcttgtgt atatcttaat tcagctacta gagtatcttc ctgaggctta
     1561 ctaggaaatt ctgccttgat atattccgaa tactgataaa tcgttttacg caaggctttt
     1621 gagcgttctc ggaggacttc ttgaggtttc ttgtgattgt aatgaacttt ggtatgagtc
     1681 gcatccacaa tgagaatgtt acttttaatc agaccgagct ctatcgcaat ctgaacagat
     1741 ttagcgatga gcaaatcaag taatcgttca tctttgttcc gaagttttcg gaatttagtc
     1801 aatgaggacg gttcaataac aggagctaga ccaaggaagt atttgaaggt cataccagaa
     1861 aaggagcgct ccaccacatc cacatcggac aacttataga tgtcttttaa taagagatac
     1921 ttgaacatca tgataaggag agagtaggaa cgacatccga agtcaggttg ataattcttt
     1981 tccagctcgt cataaataaa actaaaatca cagagttcca ttagctgtcg taaaaaatga
     2041 gttttgggaa caacaatatc acacagagta gaatagggac taacatcgat tatcaagcat
     2101 accatcacct catctttagt ataccatcat acgatgactt tttcagtgga ctccgaatta
     2161 tcttaactct tgacaagtcc agtgatgcaa tgatagagca tagacaactt taatgatttg
     2221 gggtcgaagc gtaatcgtaa aataggtaaa atcgtctttg aatgcatttt tattaatgtt
     2281 tgttttagaa gttaaaccga tattggcaac cataaacaga aaagatatcc aaaaaaattt
     2341 gagtttgtct tcagtctgat ttatatttca ttcataattt ctttattgat acactcatca
     2401 aaaaccttaa ataattgtcc aagctttctt attgttttct catactcatc aagaacatta
     2461 ccaaattcct gttgtaactt caaaagttct tcattgactt gttctaaagt tgttgattct
     2521 gtcatgttca atttagaaaa ttcatgccta gctagttttt ggctacccga accttcactc
     2581 ttagaattga taagtacttc cgccatctta tttaaggtgg caattttttc atttgtttga
     2641 gtcagtttta tatctagctc tgctatttcg gaaaccaagt tgtctttaat gtcttggtat
     2701 ttcttatctg tctcagttaa ctcaatcaag aggctaatct cagaaatctt ttcttgaagt
     2761 ctttcaacag tcggtcttct gtatggtatt actcgactat cgttggttag ctgcctaatc
     2821 aaggttctcc ctttgatata ctgattctta tccgaatgtt ctttgttata gacaaaatat
     2881 gaggttgtct cacgaatgta gactttatat tttttctgat tttcttcttc cataatatcc
     2941 agttgatagt taggaatgaa aataagacca ctctgattta taccaaagga cactttgata
     3001 taaatcccct catcaactaa cttctcaatt tggtgttctg taagttttac ttcaaattca
     3061 tgaacggcat ctcgtttttc cttaaatgtc tcaaaggctt cctcaatttc ttcagtagat
     3121 accttctcct tatctcgttc ttcttgaaaa gcacggtact gtgcctgtag attttctatt
     3181 ccttctgaaa cacctacttc cttattttta aaataatctt gaaaaaaatt gacatcatat
     3241 aatttctcat cacttatttt tt
//
LOCUS       DAVLYR010000162         3198 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLYR010000162 DAVLYR010000000
VERSION     DAVLYR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.162"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..3112
                     /gene="phtE"
                     /locus_tag="VYO63_001813"
     CDS             <1..3112
                     /gene="phtE"
                     /locus_tag="VYO63_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1672681.1"
                     /translation="SKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDGSQS
                     SQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSEELL
                     MKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEHVKD
                     NEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLSASE
                     LAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELYDSP
                     SAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPISGT
                     GSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAYIVR
                     HGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRIIAED
                     ESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLKEVKTSHNGLDSLSSHEQDYPSNAKE
                     MKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVGIGH
                     SHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVSFSF
                     PPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFKYTI
                     ASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDALVRV
                     FDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYIVEV
                     PILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTSNST
                     LEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTGEAP
                     QGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPEGNV
                     GSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVIKKN
                     LSDLIA"
BASE COUNT         1187 a          539 c          624 g          848 t
ORIGIN      
        1 cagtaaaaaa tatatagcag ctggatcagc tgttatcgta tccttgagtc tatgtgccta
       61 tgcactaaac cagcatcgtt cgcaggaaaa taaggacaat aatcgtgtct cttatgtaga
      121 tggcagccag tcaagtcaga aaagtgaaaa cttgacacca gaccagatta gccagaaaga
      181 aggaattcag gccgagcaaa ttgtaatcaa aattacagat cagggctatg taacgtcaca
      241 cggtgaccac tatcattact ataatgggaa agttccttat gatgccctct ttagtgaaga
      301 actcttgatg aaggatccaa actatcaact taaagacgct gatattgtca atgaagtcaa
      361 gggtggttat atcatcaagg tcgatggaaa atattatgtc tacctgaaag atgcagctca
      421 tgctgataat gttcgaacta aagatgaaat caatcgtcaa aaacaagaac atgtcaaaga
      481 taatgagaag gttaactcta atgttgctgt agcaaggtct cagggacgat atacgacaaa
      541 tgatggttat gtctttaatc cagctgatat tatcgaagat acgggtaatg cttatatcgt
      601 tcctcatgga ggtcactatc actacattcc caaaagcgat ttatctgcta gtgaattagc
      661 agcagctaaa gcacatctgg ctggaaaaaa tatgcaaccg agtcagttaa gctattcttc
      721 aacagctagt gacaataaca cgcaatctgt agcaaaagga tcaactagca agccagcaaa
      781 taaatctgaa aatctccaga gtcttttgaa ggaactctat gattcaccta gcgcccaacg
      841 ttacagtgaa tcagatggcc tggtctttga ccctgctaag attatcagtc gtacaccaaa
      901 tggagttgcg attccgcatg gcgaccatta ccactttatt ccttacagca agctttctgc
      961 cttagaagaa aagattgcca gaatggtgcc tatcagtgga actggttcta cagtttctac
     1021 aaatgcaaaa cctaatgaag tagtgtctag tctaggcagt ctttcaagca atccttcttc
     1081 tttaacgaca agtaaggagc tctcttcagc atctgatggt tatattttta atccaaaaga
     1141 tatcgttgaa gaaacggcta cagcttatat tgtaagacat ggtgatcatt tccattacat
     1201 tccaaaatca aatcaaattg ggcaaccgac tcttccaaac aatagtctag caacaccttc
     1261 tccatctctt ccaatcaatc caggaacttc acatgagaaa catgaagaag atggatacgg
     1321 atttgatgct aatcgtatta tcgctgaaga tgaatcaggt tttgtcatga gtcacggaga
     1381 ccacaatcat tatttcttca agaaggactt gacagaagag caaattaagg ctgcgcaaaa
     1441 acatttaaag gaagttaaaa ctagtcataa tggattagat tctttgtcat ctcatgaaca
     1501 ggattatcca agtaatgcca aagaaatgaa agatttagat aaaaaaatcg aagaaaaaat
     1561 tgctggcatt atgaaacaat atggtgtcaa acgtgaaagt attgtcgtga ataaagaaaa
     1621 aaatgcgatt atttatccgc atggagatca ccatcatgca gatccgattg atgaacataa
     1681 accggttgga attggtcatt ctcacagtaa ctatgaactg tttaaacccg aagaaggagt
     1741 tgctaaaaaa gaagggaata aagtttatac tggagaagaa ttaacgaatg ttgttaattt
     1801 gttaaaaaat agtacgttta ataatcaaaa ctttactcta gccaatggtc aaaaacgcgt
     1861 ttcttttagt tttccgcctg aattggagaa aaaattaggt atcaatatgc tagtaaaatt
     1921 aataacacca gatggaaaag tattggagaa agtatctggt aaagtatttg gagaaggagt
     1981 agggaatatt gcaaactttg aattagatca accttattta ccaggacaaa catttaagta
     2041 tactatcgct tcaaaagatt atccagaagt aagttatgat ggtacattta cagttccaac
     2101 ctctttagct tacaaaatgg ccagtcaaac gattttctat cctttccatg caggggatac
     2161 ttatttaaga gtgaaccctc aatttgcagt gcctaaagga actgatgctt tagtcagagt
     2221 gtttgatgaa tttcatggaa atgcttattt agaaaataac tataaagttg gtgaaatcaa
     2281 attaccgatt ccgaaattaa accaaggaac aaccagaacg gccggaaata aaattcctgt
     2341 aaccttcatg gcaaatgctt atttggacaa tcaatcgact tatattgtgg aagtacctat
     2401 cttggaaaaa gaaaatcaaa ctgataaacc aagtattcta ccacaattta aaaggaataa
     2461 agcacaagaa aactcaaaac ttgatgaaaa ggtagaagaa ccaaagacta gtgagaaggt
     2521 agaaaaagaa aaactttctg aaactgggaa tagtactagt aattcaacgt tagaagaagt
     2581 tcctacagtg gatcctgtac aagaaaaagt agcaaaattt gctgaaagtt atgggatgaa
     2641 gctagaaaat gtcttgttta atatggacgg aacaattgaa ttatatttac catcgggaga
     2701 agtcattaaa aagaatatgg cagattttac aggagaagca cctcaaggaa atggtgaaaa
     2761 taaaccatct gaaaatggaa aagtatctac tggaacagtt gagaaccaac caacagaaaa
     2821 taaaccagca gattctttac cagaggcacc aaacgaaaaa cctgtaaaac cagaaaactc
     2881 aacggataat ggaatgttga atccagaagg gaatgtgggg agtgacccta tgttagatcc
     2941 agcattagag gaagctccag cagtagatcc tgtacaagaa aaattagaaa aatttacagc
     3001 tagttacgga ttaggcttag atagtgttat attcaatatg gatggaacga ttgaattaag
     3061 attgccaagt ggagaagtga taaaaaagaa tttatctgat ctcatagcgt aaggaatagc
     3121 agtagaaaaa gtctgaatca aaaatgaagt tctctcaaaa gttagaaata aaactctgac
     3181 tttgggagaa tttcattt
//
LOCUS       DAVLYR010000163         3182 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLYR010000163 DAVLYR010000000
VERSION     DAVLYR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.163"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(130..384)
                     /locus_tag="VYO63_001814"
     CDS             complement(130..384)
                     /locus_tag="VYO63_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1672682.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(404..2245)
                     /gene="typA"
                     /locus_tag="VYO63_001815"
     CDS             complement(404..2245)
                     /gene="typA"
                     /locus_tag="VYO63_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1672683.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPVEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2407..3132
                     /locus_tag="VYO63_001816"
     CDS             2407..3132
                     /locus_tag="VYO63_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1672684.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
BASE COUNT          935 a          703 c          611 g          933 t
ORIGIN      
        1 gatgccatta acgaatttta ttcaagtttt tcccattcat actatacaag taaaagagat
       61 ggtgttaact aaaaagcaat tcaaactatt gtaaaattcc tagcaaaaag agagccgaaa
      121 ctctcttttt tatcttcttt tacttttttt gactggcatg agtgtgatgt ctctaacact
      181 aaagtaagct aggatcaaca tggctattgc taggaatatt tctgttggta attgaaaaat
      241 tttcagaaaa gatagaacca ataaaatcaa gagtgccact aaaatacata ccatagcgac
      301 gatattgaca gtccctttaa tgctttctgg tgtcgcaaat acatagagta ggagcagtaa
      361 aattcctagg actaaataga ccatctttct ctctttctag ctcttattca gctgattttt
      421 tcttcttgtt agctttctca cgctctgctt tgttaaggat ttgtttacgc aaacggatag
      481 actcaggcgt tacttccatg tactcatcgt cgttcaagaa ctcaagagac tcttcaagtg
      541 tcaagatacg aggcgtcttg ataacagctg tttggtcctt agtagctgaa cgaacgttgg
      601 tcatttgttt tgccttcgtg atgttaactg tcaagtcatt ttcacgagag ttttcaccga
      661 tgatcattcc ttcataaacc tcagtacctg ggttgacaaa gatcgtacca cgttcttcga
      721 tagacatgat tgagtaagtt gtagccttac cagcatcgat agaaacaagg gcaccacggt
      781 gacgtccacc aatttcccct ggaatcaatg gcaagtattg gtcgaaggta tggttcatga
      841 taccgtaacc acgagtcatt gacaagaact cagttgagta tccaatcaaa ccacgcgctg
      901 gaacaaggaa gaccaaacga gtttgaccat taccagttga aatcatatcc aacatttcac
      961 ctttacgttc agaaaggctt tggataacag acccttggta ttcttctgga gtgtcgattt
     1021 gtacacgttc aaatggttca catttaacac cgtcgatttc ttttacgata acttctggac
     1081 gagatacttg aagttcatag ccctcacgac gcattgtttc gataaggatt gacaagtgca
     1141 attctccacg tcctgaaaca gtccatttat ctggtgaatc agttgggtca acacgaaggg
     1201 aaacgtctgt ttgcaattct gcctgcaagc gttcttccac cttacgagag gttacccatt
     1261 taccttcttt accagcaaat ggtgagttgt tgaccaagaa agtcatttga agagttggct
     1321 catcgatgtg taggattgga agagcttcta ctgcatctgt cggagtgatg gtttcaccga
     1381 caaagatgtc ttccatacct gaaacggcaa tcaagtcacc cgctttggct tcttggattt
     1441 cacgacgttc caaaccaaag aaaccgaaga gttttgtaac acggaagttt ttagttgtac
     1501 cgtcaagttt agaaagggta acttggtccc caaccttaac tgtaccacgg aagacacgac
     1561 cgataccgat acgtccaacg aagtcattgt agtccaaaag cgacacttgg aactgcaaag
     1621 gctcatctga gttatctact ggagctggga tatggtcgat aatcgtgtca aagattggtg
     1681 ccatagtcgc ttcttggtca gctggatcat ctgacaatga agaagttccg ttgatcgctg
     1741 aagcataaac cactgggaaa tcaagctggt cgtcatctgc accaagctcg atgaaaagtt
     1801 ccaagacttc atccactact tctactggac gagctgatgg cttatcgatt ttgttaacaa
     1861 ccacgattgg gacaaggtct tgttccaagg cttttttcaa tacgaaacga gtttgtggca
     1921 tggttccttc ataggcatct acgaccaaga caacaccgtc aaccattttc atgatacgct
     1981 caacttctcc accaaagtcc gcgtgtcctg gtgtgtccat aatgttgata cgagttccgt
     2041 tgtaagcaac ggcagtattt ttagcaagga tggtaatccc acgctctttt tcgatatcgt
     2101 ttgagtccat agcacgctct gccaattcag tacgtgcatc aagcgtttct gattgtttca
     2161 ataattcgtc aacgagggtt gttttaccgt ggtcaacgtg ggcgataatc gcaatgttac
     2221 ggatatcttc tcttaatttt gtcatgattt cctctataat attcaaaatt tattttctaa
     2281 ctgaacgatt ataccataat ttcaaataaa taacataact caagcaagtg taaatgtttt
     2341 cactccgctt ttcttttcac gtcaagcctt ttcaaagcaa gcgacttatg ataagatagg
     2401 cacagtatgc gtttagataa tttattagct caagaaaaaa tcagccgaaa ggccatgaaa
     2461 caagcactcc tcagagggga aattctagtc gatggttgcc cagcccgctc cctagctcaa
     2521 aatatcgata caggactaca agaactcctt tttcaggatc gaatcattca aggctatgaa
     2581 cacacctatc ttatgcttca taaacctgct ggtgtcgtta cagccaacaa agacaaggaa
     2641 cttccgaccg tcatggacct gcttccatct gacatccagt ctgacaagct ctatgccgtc
     2701 ggccgactgg accgagatac aacaggactc cttctcttga ccgataacgg tcccttgggc
     2761 ttccaactcc tccatcccca atatcatgtc gataagactt atcaagttga ggttaatgga
     2821 cttctaacac ctgaccatat ccaagccttt caaaagggaa ttgtcttttt agatggtact
     2881 gtctgtaaac ccgcaaaact agagattcta tctgcaagtc cctccatcag tcaagcctct
     2941 atcaccattt cagaaggaaa atttcatcaa atcaagaaaa tgttcctctc ggttggtgtt
     3001 aaggtgacta gcctcaagag aatccaattt ggggacttca cattgaaccc agatttagca
     3061 gaaggtaact accgcccttt gaaccaaaaa gagttacaaa tcattaaaaa ctatttagag
     3121 atgagtcgat aaaataaaaa aagctttaaa actaaagctt ttttctttta tttaccgaaa
     3181 aa
//
LOCUS       DAVLYR010000164         3133 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLYR010000164 DAVLYR010000000
VERSION     DAVLYR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.164"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            121..516
                     /locus_tag="VYO63_001817"
     CDS             121..516
                     /locus_tag="VYO63_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1672685.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            527..898
                     /locus_tag="VYO63_001818"
     CDS             527..898
                     /locus_tag="VYO63_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1672686.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            908..>3133
                     /locus_tag="VYO63_001819"
     CDS             908..>3133
                     /locus_tag="VYO63_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1672687.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LN"
BASE COUNT          874 a          555 c          780 g          924 t
ORIGIN      
        1 ttttcaaatg attaaaattc tatttttcga agaaaatatt atttctataa ttgacaaatg
       61 tagattttaa gagtatactg atgagtgtaa ttgacaaatg tagattttgg aggtatggta
      121 atgcagattt cagatgcaga atggcaggtc atgaagatta tttggatgca gggggagcag
      181 accagtacag atttgattag ggttttggca gagcggtttg actggtccaa gtccacggtt
      241 cagacacttt tggctcgtct ggttgagaaa gagtgtttga ctcggaaaaa agaaggcaag
      301 tcctttgttt attcagccct tttaactcta gaccaaagtc gggatttact tgtccaagat
      361 atcaaagaca aggtttgttc ccgtaggatt aggaatttgt tggctgactt gattgtagaa
      421 tgtgaattta ctcagactga cttggaagac ttggaagctg tgatttcaga gaagaaatca
      481 agtgctgtaa cagaagtaag atgtaattgt atgtaaagga gacgtcatgt taaatagtat
      541 tgtaaccatt atttgtattg cccttatcgc gtttatcttg ttttggtttt tcaaaaagcc
      601 tgaaaaatct ggacaaaaag cccagcaaaa aaacggatac caagagattc gagtggaagt
      661 catgggaggc tatactcctg agttgattgt cctcaagaaa tcagtgccag cccgcattgt
      721 ctttgaccgc aaggatcctt caccatgtct ggatcaaatt gtttttccag attttggtgt
      781 acatacgaac ctgccaatgg gggaagagta tgtagtggaa atcacgcctg aacaggctgg
      841 agaatttggc tttgcttgtg gtatgaacat gatgcacggc aagatgattg tagagtaggt
      901 ggagactatg acagaaattg tgaaagcaag cttagaaaat ggcattcaaa aaatccgtat
      961 ccgatctgaa aaaggctatc atccagccca tatccagctt caaaagggaa ttccagctga
     1021 gattaccttt catcgtgcta ctccttcaaa ctgttataag gaaattctgt ttgaagaaga
     1081 aggtatcttg gaaccaatcg gcgtagatga ggagaaagtc attcgtttta cacctcaaga
     1141 attagggcga catgaatttt cttgtggcat gaagatgcaa aagggaagct atacagtcgt
     1201 tgagaagact cgaaaatctc tatctctcct gcaacgtttt tggattacta gtatctttac
     1261 tgtgcctctt gtgattctca tgattgggat gttgacaggt agcattagtc atcaagtcat
     1321 gcattgggga acctttctag caacaacgcc tattatgtta gttgcaggta agccatatat
     1381 ccagagtgct tgggccagtt ttaaaaagca caatgccaac atggatacct tggttgcgct
     1441 gggaactcta gtggcttatt tctatagcct agttgctctc tttgctggtc tccctgttta
     1501 cttcgaaagt gctggattta tcctcttttt cgttcttttg ggagcagttt ttgaggaaaa
     1561 aatgagaaaa aatacgtccc aagctgtgga gaaattactg gacttgcaag ctaaaaccgc
     1621 agaagtcttg agtgatgata gttatgtcca agttcctttg gaacaagtca aggtaggcga
     1681 cctgattcga gtgcgtcccg gtgaaaagat tgctgttgat ggtgtcgtag tagaaggtgt
     1741 ctctagtatt gacgaatcca tggtgacagg tgagagtctg cctgtggaca agacagttgg
     1801 agatactgtc attggctcaa ccatcaatca tagtggaacg cttgtcttta gagcagaaaa
     1861 agttggctca gagactgttt tggctcagat tgtggatttt gtgaagaaag ctcagacaag
     1921 tcgtgcgccg attcaggact tgacggataa gatttcaggg atttttgtcc cagtagttgt
     1981 cattttagga atcatgacct tttgggtttg gttcgtcttg ctcagggata gtgtggttgt
     2041 gcttggagct agctttgtgt cctctcttct ctacggagtg gcggttttga ttatcgcctg
     2101 tccttgtgcc ttgggacttg caacaccgac agcccttatg gtggggacag gacgtagtgc
     2161 caagatgggg gttctcctca aaaatggaac tgtcttacag gaaatccaga aagttcaaac
     2221 ccttgtcttt gataagaccg ggactttgac ggaagggaaa cctgtggtaa cagatgtcat
     2281 cggcgacgaa gtagaagtgt ttgggttggc agcctccttg gaagatgctt ctcaacaccc
     2341 actggctgag gctatcgtta agcgagcgag tgaagctgga cttgagtttc aaactgttga
     2401 aaattttcag accttgcacg ggaaaggtgt ttcagggcga atcaatggaa aacaagtttt
     2461 acttggaaat gctaaaatgc tggatggcat ggatatttct aatacttatc aagataaact
     2521 agaagaacta gaaaaagaag ctaagacagt tgtgttctta gctgttgaca atgaaatcaa
     2581 aggcttgctt gctttgcaag atattcctaa ggaaaatgct aagctagcca tcagtcagct
     2641 aaaaaaacgt ggtctccgaa cagtcatgct gacaggagac aatgctggtg tggcgcgtgc
     2701 tattgcagat caaatcggaa ttgaagaggt cattgcaggc gtcttgccag aagaaaaagc
     2761 ccatgaaatc cataaactgc aacagtcagg gaaagtagcc tttgttgggg acggtatcaa
     2821 tgacgctcct gcccttagtg tagcagatgt gggaattgct atgggtgctg gaacagatat
     2881 cgccatcgag tcagcagatt tggtgttgac aaccaataat cttttaggag tggttcgtgc
     2941 ctttgatatg agtaagaaaa cctttcatcg aattctactc aatcttttct gggcttttat
     3001 ctacaatgtt gtcggaattc cgattgcagc aggagtcttt tcaggtgttg gactggctct
     3061 caacccagaa ttggcaggtc tagccatggc ctttagttct gtatctgttc tgaccagttc
     3121 ccttctgtta aat
//
LOCUS       DAVLYR010000165         3109 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLYR010000165 DAVLYR010000000
VERSION     DAVLYR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.165"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            21..3026
                     /locus_tag="VYO63_001820"
                     /pseudo
     CDS             21..3026
                     /locus_tag="VYO63_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT         1184 a          461 c          635 g          829 t
ORIGIN      
        1 ttttatttca aggaggaata atggaaaagt attttggtga aaaacaagag cgtttttcat
       61 ttagaaaatt atcagtagga cttgtatctg caacgatttc aagtttattt tttatgtctg
      121 tattagctag ttcatctgtg gatgctcaag aaactgcggg agttcactat aaatatgtgg
      181 cagattcaga gctatcatca gaagaaaaga agcagcttgt ctatgatatt ccgacatacg
      241 tggagaatga tgatgaaact tattatcttg tttataagtt aaattctcaa aatcaactgg
      301 cggaattacc aaatactgga agcaagaatg agaggcaagc cctagttgct ggtgctagct
      361 tagctgctct gggaatttta atttttgctg tttccaagaa aaaggttaag aataaaacgg
      421 tattacattt agtattggtt gcgggaatag gaaatggtgt cttagtttca gtccatgctt
      481 tagaaaatca tcttttgcta aattacaata cggactatga attgacctct ggagaaaaat
      541 tacctcttcc taaagagatt tcaggttaca cttatattgg atatatcaaa gagggaaaaa
      601 cgacttctga ttttgaagta agtaatcaag aaaaatcagc agccactcct acaaaacaac
      661 aaaaggtgga ttataatgtt acaccaaatt ttgtagacca tccatcaaca gtacaagcta
      721 ttcaggaaca aacacctgtt tcttcaacta agccgacaga agttcaagta gttgaaaaac
      781 ctttctctac tgaattaatc aatccaagaa aagaagagaa acaatcttca gattctcaag
      841 aacaattagc cgaacataag aatctagaaa cgaagaaaga ggagaagatt tctccaaaag
      901 aaaagactgg ggtaaataca ttaaatccac aggatgaagt tttatcaggt caattgaaca
      961 aacctgaact cttatatcgt gaggaaacta tagagataaa aatagatttt caagaagaaa
     1021 ttcaagaaaa tcctgattta gttgaaggaa ttgtaagagt aaaacaagaa ggtaaattag
     1081 gtaagaaagt tgaaatcgtc agaatattct ctgtaaacaa ggaagaagtt tcgcgagaaa
     1141 ttgtttcaac ttcaacgact gcgcctattc caagaatagt cgaaaaaggt actaaaaaaa
     1201 ctcaagttat aaaggaacaa cctgagactg gtgtagaaca taaggacgta cagtctggag
     1261 ctattgttga acccgcaatt cagcctgagt tgcccgaagc tgtagtaagt gacaaaggcg
     1321 taccagaagt tcaaccagcg ttgtccaaag cagttataac cgacaaaggt gagactgagg
     1381 ttcaaccaga gtcgccagat actgtggtaa gtgataaagg tgaaccagca gtccaaccag
     1441 agctaccgga agcggttgtg actgacaaag gtgagactga ggttcaacca gagtcgccag
     1501 atactgtggt aagtgataaa ggtgaaccta aacaggtagc accgcttcca gaatatacag
     1561 gaccacaagc cagcgctatt gttgaaccag agcaggtagc accactacca gaatacacag
     1621 gggtacaagc cggatcgata gtcgagccag agaaagtaga agcaccgaaa gaatatactg
     1681 gtaaaatcga acagcctagt gcagaagata ctaaacctga aaatggggca agctctacaa
     1741 atggggaatc ggaacgtcct aaagataaaa ttaaagaaga aaaacaagtc gacaagaaac
     1801 tagaacttag aaatgtttca aacgtggaat tatatacggt agaaaataat aaatatcgac
     1861 atattacggc tgtagatggt gctttagata gttcattgaa gtatttcatg aaggtaaaat
     1921 cagaaaattt taaagatata atgttgcctg ttactaaaat tgaaagtaca acaaaaaaca
     1981 ataaagaagt atacaaaatt gtggctcatg cagaaaatct aattcaacac gaaaacaatg
     2041 ttatttcgaa tgactataca tattatttac caaaaactca gcaaagtgaa actggcgtct
     2101 atacatcatt taaaaattta gttgatgcaa tgaatagtga ccctaatgga acattccatt
     2161 taggtgcaac tatggatgct cgtgaagttg aacttccgga tgatcaagaa agttatgtaa
     2221 aaaatgaatt ttacggaaaa ttaataggta aaaataatgg taagtattat gctatttata
     2281 acttgaaaaa acctttattt aaaactctaa acaccgctac tattcagaat ctttcaataa
     2341 aagaagcgaa tgtttcttct aaagaagatg ctgctacaat atcgaaagaa gcaaaataca
     2401 atactcttat agataatgtt cattctgatg gtattatagc tggtgaacgt ggaattggtg
     2461 gattggtttc aaaagtagat aactcgagaa tttctaatag tagttttaca ggcagaataa
     2521 ctaacacata tgatacaact gcagggtatg aaattggtgg tttagtaggt aaactttctg
     2581 gttcgcttgc ttctatagag aaatcaatag catcgattga catagcctct aatgcaaaat
     2641 caggagatca aattgttggt ggtatagccg gagttgttga gaaaagtgcg acaattaagt
     2701 atagctatgt tgaaggaaat gttaacaatg tgcgacactt tggtaaagtt gggggagttg
     2761 caggtaatct ttgggataga gattcacaag atgtaagtaa atcaggtaaa ctatcatatg
     2821 ttttaagtga tgtcaatgtt acgaatggta atgctatagc tggctataat ttcaatggaa
     2881 taaaaacgat tgaaacatat agcaataaaa ataataaggt agtaaacgta gtacaagaag
     2941 atgatgaggt tgtgactaaa gattcagatg tacaaagagg tacggtatta gatgctgata
     3001 aagtaaaaga gaaaaagata gaattggttt ctaaacacag cactaaagta gaagattttg
     3061 attttacttc aagatacaat actaattaca atgaagtaac aggttacca
//
LOCUS       DAVLYR010000166         2957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLYR010000166 DAVLYR010000000
VERSION     DAVLYR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.166"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     regulatory      76..164
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            379..1047
                     /locus_tag="VYO63_001821"
     CDS             379..1047
                     /locus_tag="VYO63_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1672688.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYNV"
     gene            complement(1197..1283)
                     /locus_tag="VYO63_001822"
                     /pseudo
     CDS             complement(1197..1283)
                     /locus_tag="VYO63_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1374..2156
                     /gene="thiM"
                     /locus_tag="VYO63_001823"
     CDS             1374..2156
                     /gene="thiM"
                     /locus_tag="VYO63_001823"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1672689.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            2158..2787
                     /locus_tag="VYO63_001824"
     CDS             2158..2787
                     /locus_tag="VYO63_001824"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1672690.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGAITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
BASE COUNT          976 a          433 c          636 g          912 t
ORIGIN      
        1 atataaagat atttttcggt caaatatctt gctagggctt tcattttttg ctatactggt
       61 tcagtaatta aataatagag acatttgggg tgctttaggc tgagataata cccattgaac
      121 ctgatacagt taagactggc gaagggaaat gtgaacagtt ttttcgtatg gtgaaatgaa
      181 cggtctaatc ttgtaagtca actctaattc ttgattgtaa ttggagtttt ttgtttatat
      241 aaactggata ggcttgttta gttagctctc tgcaaatgct ctcgatgtgc tttaaaagga
      301 tcaagtggaa ttacgatttt gcttgatatt tcttggtgtt ttattaatta ctaacaagta
      361 aagagaggag aagtaaatat ggaaacacaa gactatgcat ttcagccagg tttgactgtt
      421 ggagaattat taaaaagcag tcagaaggat tggcaggctg caatcaatca tcgttttgtt
      481 aaggaacttt ttgcggggac aattgagaat aaggtcttaa aggactatct gattcaagat
      541 tatcacttct ttgatgcctt cttatccatg ctgggtgcct gcgtagctca cgcagataaa
      601 cttgaatcca agcttcgttt tgccaagcaa ctaggctttc ttgaagcaga tgaagatggt
      661 tatttccaaa aggcttttaa agagttaaaa gtagcagaga atgactatct agaagtgact
      721 ttgcaccctg taacaaaagc ctttcaggat ctaatgtatt cggcagtggc ttcatcagac
      781 tatgcccatc ttttggttat gctggtcatt gcagaaggtc tctatttaga ctggggttct
      841 aaagatttgg ctctacctga agtctatatt cattcggaat ggatcaatct ccacagaggt
      901 cctttctttg cagagtgggt tcaatttctg gttgacgaac tcaatcgtgt cggtaaaaat
      961 cgagaagatt tgacagaact tcagcaacgc tggaatcaag cagtcgcttt agaattagct
     1021 ttttttgata ttggttacaa tgtctagaga aggctcatga tatttacaaa tttgatgagg
     1081 taagactctt cgaaaatctc ttcaaacaac gtcagtttca ccttgccgta ggtatggtta
     1141 ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagctgac ttcgtcagtc
     1201 ttatctacag gttcaaagca gtgttttgag caacctgcgg ttagcttcct agtttgctct
     1261 ttgattttca ttgagtataa atcattcaat gtaaatgaaa aaaatttctt aacgaaagat
     1321 agatataaat cgaaatcagt agatcgtata aagtgaaaag gaaggatttc aatatgacaa
     1381 gtttaaaatt attaaaagaa aaagcaccat tggtcatttg tataaccaat gatgtagtaa
     1441 aaaatttcac agcaaatgga ttagtagcac tgggcgcctc accagccatg agtgagtttc
     1501 cagcagattt agaggatttg ttaaagtatg ctggtggttt attaataaac ataggaacat
     1561 tgacagatga aaattggaaa ttataccaag ctgctctgaa aattgcggag aaatataatg
     1621 tcccagcagt tttagatcct gtagcctgtg gagcaggaga atatagaaaa aaagtagcgg
     1681 atgatctaat caacaattat aaactagcag cgattagagg aaatgctggc gagattgcct
     1741 ctttagtagg aatagatgtg gcatctaaag gagtagatag tgcgggcgta gataatattg
     1801 acgaaattgc tctagcagca aatgagaagt tcaatattcc aatagtagta acaggtgaag
     1861 tggatgccat tgctgttaat ggagaagtgg taacgattca taatggtagt gctatgatgc
     1921 cgaaagtcat tgggacagga tgcttattag gagctgtagt agcaagcttt atcggactag
     1981 aaaaaggtca agaattgaaa tcattagaaa cagcaatgtt ggtttacaat atcgctggag
     2041 aaatagcaga aaaacgtccc aatggacatc ttcctggaac atttaaagct gaatttataa
     2101 atgccttata cgagattaca gatgaagatg taaaggaatt caaaagagtg aagtaagatg
     2161 tttcataaag aattactaaa actatatttt atttgtggaa cgactacttg ccaaggaaaa
     2221 aatctatata cagtcgttga ggaagcctta aaaggtgcta taaccttatt tcaatttcga
     2281 gaaaaaggtg agggtgcttt agaaggctta gaaaaacttg agctagctat ccaaattaaa
     2341 gaactttgta aaaaatacaa tgttccattt atcgttaatg acgatataga tttagcaatg
     2401 gaaattgacg ccgatggcgt acatgtaggt caagatgata ttggtgttga tgaaattaga
     2461 aaattgatgc cagataaaat aattggtctt tctataagaa acgaagaaga atttcaacaa
     2521 tcaaaagttg aatatgtaga ttatgtaggt gttgggcctg tatttgatac ccagtcaaaa
     2581 gatgatgctg gtggtgctat aggttatgaa ggtcttgaat tgatgagaaa actattgcca
     2641 caaatgcctt tagttgcaat tggtgggata cagacgaaac atattaaaga cattataaag
     2701 accaatgttg acggcgtttc aatcatttcg gcaatatcgt atgcaaaaaa tatagaaaaa
     2761 actgttcggg aaatgagtga acaatagata tctatccttg gattctaagt aagtaggatt
     2821 ttcctttttt aaaaagctat gtctagattc agcttgaatg gctaattcat caaacaggtc
     2881 atttgttttt tgcaaaagaa agatctgctg tctttgtgag tgcaaggctg tatttccagt
     2941 tcaatttctt gaactag
//
LOCUS       DAVLYR010000167         2884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLYR010000167 DAVLYR010000000
VERSION     DAVLYR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.167"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(16..2694)
                     /locus_tag="VYO63_001825"
     CDS             complement(16..2694)
                     /locus_tag="VYO63_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1672691.1"
                     /translation="MAVFVSLDGIVVEVLDVFSSFDGDSEFFLCKRLKDKSQFVMERS
                     RFEEMFQLQSSHLTTQEKLQLFTSVFAGRYDVYAKNFINEQGKIQYFPSYDYGWKQLP
                     PEKRSFQTLTNSVLKSHFRGETAIGIFPMHLDDSCHFLVLDFDEGDWKEAGLTIRRIA
                     RERQMEAHLEISRSGHGLHIWFFFEEAIPSREARLFGKKLIELAMQESMQLSFDSFDR
                     MFPNQDVLPKGGFGNLIALPFQGEAYHQGRTVFVDEQFQPYEDQWRYLQEIQRVSTAK
                     VALLIQEELGKQELDKGLKIVLSNMIQLEKSSVTPKTLFFLKNMASFSNPEFYLKQAM
                     RQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVVVEDRRKVQRSISVEFKGEL
                     TFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKTKTIILVHNKQLLDQWLDR
                     LNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKLVDVVMIQSLFKLENSQSL
                     LDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPERKNGHEPIVFQRIGEILH
                     TADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWIATDSARNQLILKDILAQV
                     AEGRNILVLVNRIQQIDVFEKLLKEKEVDDCYIISGKTKVRERTSLLETLEQLDKGFV
                     LLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIHRNYKDKSLVRIFDYVDIH
                     VPYLEKMFQKRQVAYRKMDYRAIEGEEKQFVYVDSRYEKVLIEDLAGERQECLLILPY
                     VHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKIKVSFTQSKIVTPILLVNK
                     TIVWYDAMPLLGKVDEMTILRLESASIVSELVAGLR"
BASE COUNT          908 a          678 c          437 g          861 t
ORIGIN      
        1 ttttaaaaat tttctctatc gtaaacctgc cactagttca gaaactatac tagctgattc
       61 caaacgtaat atggtcatct catctacctt ccctaataat ggcattgcat cataccaaac
      121 aatagtctta ttcaccaaaa gaataggcgt tactattttt gattgagtaa aagacacttt
      181 aattttctgg ctcttcaact ggtctagcca tgctttattt gcaaccgtct ctggtataca
      241 tatctcaatt tgactaatcc taaattcttt tagaaaattc atcagttttg tctggtgcac
      301 ataaggtaaa ataagcagac attcctgtct ttcccctgct aagtcctcta tcaacacctt
      361 ctcatatcta ctatcaacat aaacgaattg tttctcctca ccctcaatgg cacgataatc
      421 catctttcga taagctactt gtcgtttctg aaacatcttt tctaaataag gaacatgaat
      481 atccacataa tcgaaaatac gcaccaaaga cttatccttg tagtttctat gaatccgacc
      541 tgcatactga attaaattat ttttccaaga aaagggtgct gccaagataa gcgtgtccaa
      601 ctgaggtaag tcaaaacctt cgccaatgta ttttccagta gacaacaaaa caaacccttt
      661 atctaactgt tctaacgtct ccagtaaact cgttctttct cggactttgg tttttccgct
      721 aataatgtaa cagtcatcaa cctctttctc tttcaataac ttttcaaaga catctatctg
      781 ttgaattcga ttaactaaaa ccaagatatt ccgtccttct gccacttggg ctagaatatc
      841 cttgagaatc aactgattcc tcgctgagtc tgtagcaatc caatcactga gctgtataaa
      901 attacttgct ttggtctttt caatctccaa atgaccaaaa gaagtgaatc ttaattgcaa
      961 ttgccgttta aaatccgttt ccctcttatc agcagtatgg agtatctcac caattctctg
     1021 aaaaacaata ggctcatgac cattcttacg ctcaggcgta gccgtcaaac cgtaaagata
     1081 cttccctcta aactgagcaa caactttttc aaacatcaag gcagagacat gatgacactc
     1141 atccacaatc atcatctcat actcatccaa aagactttga ctattttcca acttaaatag
     1201 agattgaatc ataacgacat caaccagttt actcagccat ttcttagtcc caccgtactg
     1261 tccaacatag ccgattacct tttcacgacc tgatgccgta taacggatag cctcctcctc
     1321 ttcgaaagtc aaaaagcagt ttaagcgatc tagccattgg tctaagagtt gcttattatg
     1381 gactagaata attgtttttg ttttccgttc agagataaga gcagcaccta aaacggtctt
     1441 cccaaaacca gtctccgcat gaagtaaacc attttcttta gaagtcatat ctgacagggc
     1501 taactcttgc tcaaaagtga gttctccctt aaattccacg ctaatagacc tttgtacctt
     1561 tctcctatct tccacaacta cctgcttaaa tttatcttgc aatggataca gcaaacctct
     1621 tggcaaccat aaataatgat cggattctcc aaataaatac attctctcag gaatttgata
     1681 ggttggctgt cgcatagcct gctttaaata aaattcggga ttagaaaatg aagccatatt
     1741 tttcaagaaa aacagtgtct tgggtgtcac agacgatttt tcaagttgga tcatattgga
     1801 taagacgatc ttcaacccct tgtccaattc ttgcttgcct aactcttctt ggattaacag
     1861 tgccacttta gcagttgaaa ccctctgaat ttcttgtaga tacctccatt ggtcttcata
     1921 aggctgaaac tgttcatcca caaagaccgt tcgcccttga tggtaagctt ctccttgaaa
     1981 aggcaaggca atcaaatttc caaatccccc cttaggaagg acatcctgat ttggaaacat
     2041 gcgatcaaaa gaatcaaagg acagttgcat actttcctgc attgccagtt ctatcagttt
     2101 ctttccaaac aagcgagcct ctcgactcgg aatcgcttcc tcaaagaaga accaaatatg
     2161 gagtccgtga cccgaacgag aaatctctaa atgggcttcc atctggcgtt ccctggctat
     2221 tcttcgaatg gttaaaccag cttctttcca atctccttca tcaaaatcca gtaccaaaaa
     2281 atgacagcta tcatctaagt gcataggaaa gataccgata gctgtctccc cacgaaaatg
     2341 agatttcaaa acggagttcg tcaatgtctg gaaactccgt ttttcaggtg gcaactgctt
     2401 ccaaccataa tcataggaag gaaaatactg aattttccct tgttcattga taaaattctt
     2461 agcataaaca tcataacgtc cagcaaacac ggaggtaaac aattgtaatt tttcttgcgt
     2521 cgtcaagtga ctactttgaa gttggaacat ctcctcgaac cgagagcgtt ccataacaaa
     2581 ctgactcttg tctttaagac gtttacacaa gaaaaactca ctatccccat caaaagaaga
     2641 aaagacatca agcacttcta ccacaattcc atccaaagac acaaaaacag ccataagagt
     2701 cacctccttg attcctatag gctgattata acaagaatag ctgaaattgt acacgaaaat
     2761 aaaatccgaa tagcactcat tttgtatgcg actaatagtc catctcgctc cggaatgtac
     2821 gaggtaaata gagtttaaac gagcagtcta taaaattcaa aaaacgcata ttaccaaggt
     2881 tgtt
//
LOCUS       DAVLYR010000168         2878 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLYR010000168 DAVLYR010000000
VERSION     DAVLYR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.168"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VYO63_001826"
     CDS             complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VYO63_001826"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1672692.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1420..2196
                     /gene="recX"
                     /locus_tag="VYO63_001827"
     CDS             1420..2196
                     /gene="recX"
                     /locus_tag="VYO63_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1672693.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDENIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2285..2818
                     /locus_tag="VYO63_001828"
     CDS             2285..2818
                     /locus_tag="VYO63_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1672694.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          911 a          530 c          522 g          915 t
ORIGIN      
        1 cttttcaaac tttgtcaact acttttttaa acttttttta ttaattttac aacagcttca
       61 gttcgagctg tatgtgggaa catatcgacc gactggatat aatgaagatc atagacttct
      121 actaagcgta ccaaatcacg agccaaggtc gaaacattac aagaaatata aaccattttt
      181 tctggtacat aagtaagaat agtatctaat aacttatcat ccagacctgt acgtggtggg
      241 tcaacaatca aagcatctgc tcggtagcct tccttgtacc aacgaggaat aatctcttct
      301 gccgttccag cttcataatg agtattgtca aatcccattc ttttagcatt tcgcttggca
      361 tcttcaatag cttctggaat aatatccata cctctgagtg tttttacttt ctttgcaaag
      421 gcaaatccaa tcgttccaac tccacaataa gcgtcaatca aatggtcttc tttatcaaca
      481 tccagcgctt ttactgcttc gctatagagg acttctgttt gctcaggatt tagttgataa
      541 aaagctcgag gggatagtga aaattcataa ttgagtacac cttcttgaat actctcttgc
      601 ccccagataa tctctgtctt ttcaccatat atctcactgg ttttagctgt atttgtatta
      661 acagctactg tcacaacttc tgggaaatct ttaaccaact cttttaccaa ttgagttaaa
      721 ttaagctggc ggtttgtaac aataataatc tgaacctgtc cggtctttct cgcgcgtcgg
      781 accataatag tacggacacc tagaactttt ctctcatccg tgattggaat ctggtgataa
      841 gtaagtaatt ctgctaagcg attagcaatc acttgggttt ccttatcttg taccaggcag
      901 tctttcaact ctactaaata gtgagagttt tgtgcatata agcccgcctt gacctgattt
      961 ttaaattttc gagtctgaaa ttgtaactta gctctgtaat attttggttc ctgcattcca
     1021 atagttggac gaatttcata attttcatat cctgcaggag caaatttttt cagcgcttga
     1081 tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat gcaggtgcat gatttggcag
     1141 cctccgcatt cattataaat agtacaagat ggcacaattc gaaatttaga cttcttgttg
     1201 accttcagta attttgcttc aacaaagttg cgtctaatag aagtaatctg acaatagata
     1261 tcttcgcctt tgagagctcc aggtacaaag actaatgttt tttggtaaaa gccgattccc
     1321 tcaccattaa ttcccatgcg tttgattttt aatggtattt tttgtttcac tttcagattc
     1381 atacccctat cttatcacat tttgagttat aatagagcta tgaaaatcac aaaacttgaa
     1441 aagaaaaaaa gactctatct gatggagctt gataatggcg acaaatgcta tatcaccgaa
     1501 gatacaattg ttcgttttat gttatcgaga gataaggtga taagcgaaga ggaattgaaa
     1561 gagattcaag actttgctca attttcttat ggtaagaatc tggccctcta ccacttatcc
     1621 tttaaagcac gcactgaaaa agaagtcaga gaatatctga aaaaatacga tattgatgaa
     1681 aacatcgttt ctcaagttat tgctaatctt aaagaagata agtggattaa tgatggtcag
     1741 tacgcttatg ctatcatcaa taccaatcaa ctttcaggag acaagggtcc ttatgtgctg
     1801 actcagaaac tagctcaaaa agggatttca aaatctacta tagaagagaa cttgaaagaa
     1861 tttgattttt ctgaagttgc tcaacgtgta gccaataaac tattgaaaaa atatgaggga
     1921 aaacttccag ctcgtgccct gcaagataag attatccaga acttgactaa caagggcttc
     1981 tcttattctg atgctaaaat tgcctttgac gacttagata gccaagttga ccaagaaacg
     2041 actcaagaac tcatcttcaa ggaacttgat aagcaatata ctaagtatgc tcgaaagtat
     2101 gaaggatacg aactgaagca gcgtttaact caagttttag cacgaaaggg ctacgatttt
     2161 tcggatatag caagcgctct tagagaatat ctttaatatt tccaagtgaa attcacagat
     2221 tttaggtaat tttatggtac aatagtaaat gataaactta taaattgtag aaagttggtt
     2281 agttatgaag cttccaaaag aaggcgactt tattacaatt caaagttata agcatgatgg
     2341 gagtctccac cgaacttggc gggacaccat ggtactaaaa acaacagaaa acgccattat
     2401 tggtgtcaac gatcatacac ttgttaccga aagtgatggt cgtcgttggg tcactcgaga
     2461 accggctatt gtttactttc acaagaaata ttggtttaat atcattgcca tgattcgtga
     2521 taatggaact tcttactatt gcaatatggc tagcccctac tatctggatg aagaagcact
     2581 gaagtatatt gattacgatt tggatgttaa aatttttaca gatggggaaa aacgtctctt
     2641 ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat tattctgatg acttggacta
     2701 tattttaaaa gaacatgtca aaattcttgt tgattggatt aacaatggac gtggtccttt
     2761 ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta gaactaaaga atcggtaaag
     2821 ttgtcaaagg tcaggaacaa aatcctgacc tttttttggt agtataaaat aaattgtg
//
LOCUS       DAVLYR010000169         2871 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLYR010000169 DAVLYR010000000
VERSION     DAVLYR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.169"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            74..304
                     /locus_tag="VYO63_001829"
     CDS             74..304
                     /locus_tag="VYO63_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1672695.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            533..2791
                     /locus_tag="VYO63_001830"
     CDS             533..2791
                     /locus_tag="VYO63_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1672696.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKTEVKSSEKEK"
BASE COUNT          909 a          598 c          593 g          771 t
ORIGIN      
        1 tcttagtttt ttctagtcaa gttggcctcc tttatggtaa aatagtaacg ataagaaatg
       61 aaggagaatc aaaatggaat cacatttggt tagaatcatc aaccgccttg aagcaatggc
      121 aaacgatggc ggaaatttaa aacgtaattt tgaacgcgaa ggagttgttg ttgcagaagt
      181 tgcgtacagc cacgacgaag aaaacggccc aatctttacc cttcgtgacg tagaagatcg
      241 cgaaacgtat acgtttgata gcattgactt aatcgcaatg gaaatttacg aacttctcta
      301 ctaatctaaa acaagctggg attgtccctg catgtctaag taaaatcctt tttgtctcac
      361 aaataggatt tttttatagt ccaaattcac aaaaattttc attgtaacaa cttctcaaag
      421 cttcaatctt tttacttgct ttacacccca atcctgttat aatgagagta tagaatttga
      481 ctatttttga cctttaaaca agtcaggcta aagaaagaaa tgaggtagat gtatgctttg
      541 tcaaaactgt aaaattaacg actcaacaat tcatctttac accaatctca atggaaaaca
      601 aaaacaaatt gacctctgtc aaaactgcta taagattatc aaaacagatc ctaacaatag
      661 cctcttcaaa ggtatgacgg atctgaacaa tcgtgacttc gatccctttg gtgatttctt
      721 caatgatcta aacaatttca gaccttctag caatactcct cctattcccc caacccaatc
      781 aggtggaggt tacggtggaa acggcggtta tggttcccaa aatcgtggat ctgctcaaac
      841 tccgccacct agccaagaaa aaggcctgct ggaagaattt ggtattaatg taactgaaat
      901 tgcccgtcgt ggagacattg accccgttat tgggcgcgac gatgagatta tccgtgtcat
      961 cgagattctc aatcgtagaa ccaagaataa tcctgtcctt atcggtgaac ctggtgtcgg
     1021 aaaaacggcc gttgtcgaag gtctagctca gaaaattgtc gatggcgatg tgccacataa
     1081 actccaaggt aaacaagtca tccgtctgga tgtggttagc ttagttcaag gaacggggat
     1141 tcgaggacaa tttgaagaac gcatgcaaaa actcatggaa gaaattcgca aacgtgaaga
     1201 catcatcctc tttatcgatg aaatccatga aattgttggt gctggttctg cgagtgatgg
     1261 taatatggac gcaggaaata tcctcaagcc agcccttgct cgtggagaac tgcaactagt
     1321 cggtgctact accctcaatg aataccgtat cattgaaaag gatgctgccc tcgagcgtcg
     1381 tatgcagcct gttaaagtcg atgaaccaac ggtggatgaa acaatcacta ttctcaaagg
     1441 gattcaaaag aaatacgaag attaccacca cgttcaatat accgatgctg cgattgaagc
     1501 agctgcaact ctttccaatc gctacatcca agatcgcttc ttgcctgaca aggccattga
     1561 cctcctagat gaagctggtt ctaagatgaa cttgaccttg aattttgtgg atcctaaagt
     1621 aattgatcag cgcttgattg aggctgaaaa tctcaagtct caagctacac gagaagaaga
     1681 ttttgagaag gcggcctact tccgcgacca gattgccaag tataaggaaa tgcaaaagaa
     1741 aaagatcaca gaccaggata ctcctatcat cagcgagaaa actattgagc acattatcga
     1801 gcagaaaacc aatatccctg ttggtgattt gaaagagaaa gaacaatctc aactcatcca
     1861 tctagccgaa gatctcaagt ctcatgttat tggccaagat gatgcagtcg ataagattgc
     1921 caaggctatt cgccgtaatc gtgtcggact tggtacccct aaccgcccaa tcggaagctt
     1981 cctcttcgtt gggccaactg gtgtcggcaa gacagaactt tccaaacaac tggctatcga
     2041 actttttggt tctgctgata gtatgattcg ctttgatatg agtgaataca tggaaaaaca
     2101 tagtgtggct aagttggtcg gcgctcctcc aggttatgtt ggctatgatg aggctggtca
     2161 attaactgaa aaagttcgcc acaatccata ttctctcatc cttctcgatg aagtggaaaa
     2221 agctcaccca gatgttatgc acatgtttct tcaagtcttg gacgatggtc gtttgacaga
     2281 cgggcaagga cgcaccgtta gcttcaagga tgctatcatt atcatgacct caaatgcagg
     2341 tacaggaaag accgaagcta gcgttggatt tggtgctgct agagaaggac gtaccaattc
     2401 tgtcctcggt gaactcggta acttctttag cccagagttt atgaaccgtt ttgatggcat
     2461 tatcgaattt aaggctctca gcaaggataa cctccttcag attgtcgagc tcatgctagc
     2521 agatgttaac aagcgcctct ctagcaacaa cattcgtttg gatgtaactg ataaggtcaa
     2581 ggaaaagttg gttgacctag gttatgatcc aaaaatggga gcacgcccac ttcgtcggac
     2641 tattcaagac tatattgagg acacaatcac tgactactac cttgaaaatc caagcgaaaa
     2701 agatctcaaa gcagttatga ctagcaaggg aaacattcag attaaatctg ccaaaaaaac
     2761 tgaagttaaa agttctgaaa aagaaaaata aatcctataa aaaaagagta gaaaatgaaa
     2821 tttttctgct tcttttttta ctaaaataac tgtaatttct tgacagcttg c
//
LOCUS       DAVLYR010000170         2869 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLYR010000170 DAVLYR010000000
VERSION     DAVLYR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.170"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(137..1063)
                     /locus_tag="VYO63_001831"
     CDS             complement(137..1063)
                     /locus_tag="VYO63_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1672697.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYYKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(1056..1748)
                     /gene="ftsE"
                     /locus_tag="VYO63_001832"
     CDS             complement(1056..1748)
                     /gene="ftsE"
                     /locus_tag="VYO63_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1672698.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(1766..2861)
                     /gene="prfB"
                     /locus_tag="VYO63_001833"
     CDS             complement(join(1766..2788,2790..2861))
                     /gene="prfB"
                     /locus_tag="VYO63_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1672699.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
BASE COUNT          842 a          662 c          484 g          881 t
ORIGIN      
        1 aagttcaaat tagccatcaa gaagtcttct ctgggggact tctttggaag ctcttttctt
       61 ctgcgcatct cttttcaaaa actcttgtag caaaaaagga gattttacaa tctcctcata
      121 aaagcagcta ttttacctaa atcttcaaga atcggcgcat ggatattcct gatcccaatg
      181 aaccaatgaa aaccccaatc acaaatagta gggcaatcat caacggacta aataaatctg
      241 gactaatcat ggatagattt tgccctacca acgatttgtt gacagattga taaacaattt
      301 gataaacaat aaagaccaaa acagatggtg cgatagctcc caataaaccg ataaaggctc
      361 cttctaacaa gaacggtcca cggatataac tgtttttagc tccgaccaag cgcatgattt
      421 gaatttcgcg actgcgggaa ataatggtaa tacgaatggt atttgaaatc aaaaaaactg
      481 cgataaaaat taacaaagca gcaatcccta gtccccaaac acggataaat gaagctaact
      541 tgaagagtct ttctgtattg gcaccgccat cttgaacctc agagacacct tcaatttttt
      601 tagcatcttc ggctatagtt tttacatcat ttggagtgtt tgcctctaca atataggcat
      661 catagagagg attggcatct ccttcaaaga ttttccagtt atctcccatt atctcggtta
      721 atttttcata ttgttcttct ttacttgaaa aggtaacact tttaaccgta gacatgttct
      781 tcaaagaatc atataccttg tagtagtcat tatttgtaac agtttgacct tctttttcaa
      841 ttgtctgact attatcttcc acatcctttc ggatataaac tactacacgg acattatttt
      901 caatatctgt agctagtttc gctgtattga aaataacaga tgcaaatatt gccaccaagg
      961 tcaaagtaat catgactgaa ctgacagcag ctactgtcat ccaaccattt cgtttcaaac
     1021 tttttaaggc ttcaaataaa tggcgaaaaa atctactaat catcgtatcc atactctcct
     1081 tttgattcgt cacgaacgac acggccattt tcaatggcaa tgacacggtg gcgcaaggta
     1141 tttacaatct ggctattatg agtcgccatc aaaatagttg ttccttgtag gttaatccgt
     1201 tccaagagat tcataatttc ccatgaatta tccggatcca gatttcctgt tggctcatca
     1261 gctatcaata ctttgggatt atttacaatt gcacgcgcaa tcgcaattcg ctgttgctcc
     1321 ccacctgaga gttcatttgg aaaagaacga accttatgct tcaatccaac caagtccaaa
     1381 acttccatca ctcgtctttt gatattacgg cgattttccc cgattacttc catagcgtaa
     1441 gcaatatttt catagacagt tttctttggt aacagtttat aatcctggaa gacaacccca
     1501 acactacgac gtagaagcgg gacatctttc tttttgatct taaccagatt aaaaccagca
     1561 actgataggc ttcctttatc gatttttact tcacgataca gagaacgaat aaaagttgac
     1621 ttccctgctc ctgaaggtcc tacgatgtaa gcaaattccc ccggttgaac gctaaccgaa
     1681 acaccgcgta gagcagttgt tccgttgtcg tattttttaa cgacatctct catttcaata
     1741 attgacatgt gagttccttt ctatcttagc taattcgcca cttgagataa gcatcgataa
     1801 aaccatctag gtccccatcc ataaccttat ctacctgagc aacctcaaag ctagttcggt
     1861 gatcttttac catagtataa ggcgtgaaga cataagaacg gatttggctt ccccaagtga
     1921 tctccttttt ctcacctttg agagaatcta cctccgcagc cttcttatct tgctccattt
     1981 gatagagctt agcctgcaac atcttcatgg cacgatctct atttccatac tgggtacgat
     2041 ccactgttga ttggacaaca attccagttg gaatgtgggt taaacgtaca cctgttgaaa
     2101 ccttattgac gttttgtcca ccggcaccac ctgaacggaa ggtatccatc ttgatatcat
     2161 cttcacggat ttccacttca atagtatcat ccaattctgg catcacttct acagatgtga
     2221 aagaggtatg gcgacgtttg gcagagtcaa atggtgagat tcgcactaag cggtgaacac
     2281 ccatttctga cttgaggaga ccataggcat taggcccttc aaatgataaa gttaccgact
     2341 taataccagc ctcatcacct gcttggtaat ccaacacttc cactttaaag cctttagcat
     2401 taccataacg agtatacata cgaagcaaca tatcacccca gtcctgcgcc tcagtaccac
     2461 cagaacctgg atggatttcc aagatggcat tgttgtggtc ataaggttct gacaagagta
     2521 gagtcatctc gtagctggtc attatcttat caagttcggc taactgcgct accagttcat
     2581 catgcactga ctcgtcttca gccaaaaaat ccaataaaat ttcgacttca tcctgcaact
     2641 cttccatctt atggaaggta ttgtaagtgt tttttaattc atttaattct tgcgacgttt
     2701 tttgggccgc aatattatcg ttccaaaaat caggttctgt catcttgttt tccaagatgg
     2761 caatctcttc ctctagccct tcgaggtcaa agagaccccc tgaaagaagc taatttttca
     2821 cgatttgcgt caattttctg acgaattact gaaatgtcca taaatactc
//
LOCUS       DAVLYR010000171         2864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLYR010000171 DAVLYR010000000
VERSION     DAVLYR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.171"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(323..1633)
                     /locus_tag="VYO63_001834"
     CDS             complement(323..1633)
                     /locus_tag="VYO63_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1672700.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALKLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1870..2742
                     /locus_tag="VYO63_001835"
     CDS             1870..2742
                     /locus_tag="VYO63_001835"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1672701.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          866 a          490 c          665 g          843 t
ORIGIN      
        1 ttagaaatcg atttgactat cctgatctat ttgtcctgtt cttatttcat ttcactacat
       61 ctcaaattga gtatgacgaa gtgcgctccc atgtcctggg aacgcacttt cttcatattt
      121 ttcatattct tgaatccatc gataaagact attgggatga atttttaaag ttgaactaat
      181 tatttttaca ggatgaggtt tacagcagag agtttgaagg ctttatcaaa ggtttttctt
      241 ggcataatga cttttcctcg tttccactta attttgtgtc tactttatta taccaagtcc
      301 acccttaagt tagataatga atctaactta aggaagctag aaggatgaga atccaggtgg
      361 tcaagagtcc caaacttaag ctgataggga cacccagaat aatttgcttt ttgaaggcaa
      421 gaccgcgttc ctctatattg ggaagtgaga gttgaatgag agaaccagct gatgaaaagg
      481 gtgagatatt agtagataga gcgccaataa cggtggctgt tgtgagtaag tgaatatcaa
      541 tctgaggatt ttgagcactg atgatagcaa tgatgggaaa gagggctgga gctacaacgg
      601 atagggtgga actaaagagt gacatcactc cggctatcac acaaaagaac agaggtaacc
      661 agaaatgagg aatggttgtt gtcatgaggt gccctatcag tgtgactaaa cctgacttga
      721 ccgctagaga cattagtaag ctcatgccgc agagcatgat aattgtagcc cagggaacct
      781 tagctaaaat ggcttcttgc ttccctaatt tgagctttaa ggcgaggcag accatgagta
      841 ttgagacaaa gccaatatca aatgtttttt gataagtagc tatccaggcg atgtttggga
      901 aaatgagatg caacaaggga aaaagccaaa ccaaaaccat gctgctgatc atgagcaagg
      961 tggtttgtct ttgaaccttg ctgaggagtg gtggttggtc aatagtcaag gatgagtttg
     1021 ttcttccctt actatagtga ctgtaacagg ataataaaag caagacgatg agtgggtaga
     1081 taatgctgac gataaagata tgattgccaa gtgaaaaagc ttgctcttcc catcccattt
     1141 gcttaaacag gccttgaaag acaatgcctg agctactggt tatcaaatta gcccctcctg
     1201 aagctcccca attgacggct tgagctccaa tcaaagggtg tttgtccgct ttttgacaga
     1261 gggtaatcgc tagaggacag caaacggcca tagtagtgaa aaatccagca cctaaagcag
     1321 acaaaagggt tgccatcagg tataaaatca tgtagagggc gttagggtgg gtgcgtgtgc
     1381 ggtagagaat gtgttgagcc aaaacatcaa gagtaccgtt agttgttgca acgttataaa
     1441 agagagagac gctaaaaatg gtaaaaaaga gtgaggttgg ccaaaaatga agaagttctt
     1501 tggggcttaa tcccatgaga gtggttgcga tgaggtaaga aaaagcaata gccagcaggc
     1561 caatattgat tttggtgcgg taaccaattc caatggctag agcaatggcg ctaatcatta
     1621 ttaaatgaat cattgaattg tcctttcagt tagaatatgg aaataggttg gcaattttgg
     1681 cgctcaaaag accacctgtt tcctattggt acttgactat tatacacaaa aaaaatacaa
     1741 ttgtcttgac aattgtattg acacttgtta gaattaaaaa tgaggtattc aggcttgtga
     1801 tttcctatgg aagttaatag taattgcctc tagtgcttac aagtgatatt aaaaatagag
     1861 gacttagtga tgtcaatcat ttcaactgat ttaacccctt ttcaaataga tgatacattg
     1921 aaagcagcct tgcgagaaga tgttcattcc gaagattaca gtaccaatgc catttttgat
     1981 catcatggcc aagctaaggt gtcgcttttt gccaaggaag ctggtgtttt agcggggcta
     2041 accgtttttc aaagggtttt taccctattt gatgccgagg tgaccttcca gaatcctcat
     2101 caatttaagg atggggatcg tttgactagt ggcgatttgg ttttagaaat cataggctcg
     2161 gtgagaagtc tcttaacatg tgaacgcgtt gccttgaatt ttttacaaca tttatcaggg
     2221 attgcttcga tgacagctgc ttatgtagaa gccttaggcg atgattgcat taaggtattt
     2281 gatactcgaa aaactactcc taatttacgt ctttttgaga aatatgccgt gagagttggc
     2341 ggtggctata atcatcgctt taatttatca gatgctatca tgctaaaaga caatcacatt
     2401 gcggcagtag gtagtgttca aaaggcaatt gctcaagcgc gtgcctatgc cccttttgtg
     2461 aaaatggtcg aggtggaagt ggaaagcctt gctgctgccg aagaagctgc ggcggcgggt
     2521 gctgatatta tcatgttgga taatatgtca ttggaacaga ttgaacaggc cattacccta
     2581 attgcaggac gttctcggat tgaatgttct ggaaatattg atatgaccac tattagccgt
     2641 tttcgtggtt tagcgattga ttacgtctcc agtggtagtt taacccatag tgctaagagt
     2701 cttgattttt ccatgaaggg gttaacctac cttgatgtct aagttgtaaa ataaactaac
     2761 tttttaaagg atgtctttcc tctagaacga gttttatgtc agatagttta aacgcctctt
     2821 caaatatagt aaaatgaacc aaaaatagta cacaatgtgg tata
//
LOCUS       DAVLYR010000172         2840 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLYR010000172 DAVLYR010000000
VERSION     DAVLYR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.172"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(100..657)
                     /gene="sdbB"
                     /locus_tag="VYO63_001836"
     CDS             complement(100..657)
                     /gene="sdbB"
                     /locus_tag="VYO63_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1672702.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(668..1375)
                     /gene="ccdA2"
                     /locus_tag="VYO63_001837"
     CDS             complement(668..1375)
                     /gene="ccdA2"
                     /locus_tag="VYO63_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1672703.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(1606..2022)
                     /locus_tag="VYO63_001838"
     CDS             complement(1606..2022)
                     /locus_tag="VYO63_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672704.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLDVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            complement(2047..2247)
                     /locus_tag="VYO63_001839"
     CDS             complement(2047..2247)
                     /locus_tag="VYO63_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672705.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            complement(2414..2840)
                     /locus_tag="VYO63_001840"
                     /pseudo
     CDS             complement(2414..2840)
                     /locus_tag="VYO63_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          840 a          583 c          510 g          907 t
ORIGIN      
        1 ataattctat tttaccataa atataggaga tatgtgaata tttataaaat gtttttctaa
       61 aaaaatctga ctacatcttg taatcagatt ttatcgattc tagttcattt ctttaaatgc
      121 tgcttctgca tccgcattac tgatagcacc aaattgaatc tttccaatct ttccttggct
      181 atcaattaaa tattctgtag gaatgcttcg aatttgataa gcttggaagg tggttgcttt
      241 ggtatcataa agaactggga tatccttata tccttgttcc tggaaccatt gtgggaattg
      301 ctcaacagtt ttttcacctt gaattcctgg tgcaatgaca gtaagaattt cgaaatcacg
      361 atctggtttc gccgctagtt ccatcaactc tggcatactt ttcttgcatg gaccacacca
      421 tgaagcccaa aacttcaagt aaaccttttt acccttaaaa tcagataact taacttcttt
      481 gccatccatg gattgcaatg tgaagtctgg agcatctttt ccaacagcaa tttgttgtac
      541 agtcgtttgt tgttttggct gttgtgctgc ttgagtcttt ttagtttctt cctcaccaca
      601 ggccatcaat acaactaatg acaagagact taagccagca aacattactt ttttcatttg
      661 tcctccttta ttcaaaaatt ccagctagaa catttacttg tcctaatagt aacaaaattc
      721 ccattaaaac aatgaggaaa ccaccaattt tctttagtag catcatatga cgcttgattt
      781 tactaaaata tggcatgact agacctgaag ctagtgccaa gaccaagaaa ggaagggcca
      841 tgcccagagt gtaaatgaga gtataaatcg ctccttgcca agcaccattg cctccagaag
      901 ccgcaagtgc taaaacagaa cttaaaactg gaccaataca aggtgtccaa ccaaagctaa
      961 aggtaatacc aagtaaaaaa gctgaccaat aacgattaga atctgatttt ttaaaggtaa
     1021 aacttttttg aacttctaat ttcttcaaat gaaaaatttc catctggtga agacccaaaa
     1081 tgataataat agctcccatg acatatcgaa accaatttgc atagagaata tgaccaaagt
     1141 aaccagcacc aaagcctaga ataaagaaaa tgagagagat accagcgata aagcaaagtg
     1201 ttcgaatcaa gcctgaccag agaacctttc tcccaaacaa agaaaagctt tttgcacttt
     1261 cttgatcatc caataaaatc ccagcataga ctggcagaag aggaaaaata caaggagaaa
     1321 aaaaggataa aacacctgct agaaaaacag agattaaaaa tactatcgtt tccaataaag
     1381 aaccaacttt cttaataatt ctaatcctat tttactatat tcaattttat ttgtaagctt
     1441 tctgctacgc aaaatcgtat cgggcactat tggaccaatc ttttcttttg ctagtcaagg
     1501 cggatcttat cccccaaaat agccaaaaag caacgacaag gattactcgt cactgctttt
     1561 gtgaacgaaa atgtctttta ggtctgacat ttcataaatc atgttttact tgagtttgtc
     1621 aaggattgct ttaagctcct ctactagttt agtttctgtc tctgctgagc cattttcttc
     1681 tttcacgaaa tcaagggttt cttggagaag gttttgggct ttggcaagga cttttttatc
     1741 cgctttttct gcatctagct gtcctagaac cttgatcaat tccgtgctta attgctggat
     1801 ttctgactct ttcttacggc gaatcagcca gaaggcaatc acgtctagga gggcaagtag
     1861 actgaccaca atcactcctg ccggaactga gtttgtttca gtcatcttat ctgaatcctt
     1921 actatcttcc gttccttgtt ttgcatcctt cttgtcctgt gcaggcttgc tgtcgctagc
     1981 atttgctttc acatctttga gagagtccaa ggcagccttc acagactcta ctgcagtatg
     2041 cagaccttac tctgtcaagg cactatcttc cggagctttt tgagcatcta ggaggacagc
     2101 cttggttgca tcgatttttg gatcagatac tgttgccaaa gctttcaagc gttggtctaa
     2161 ctcttgactc aaggcacgaa gttcagactt gtcaacttgc tcttgagctt gtgtgctcgt
     2221 tgagctagcc gaagcgcttg ctaccactct aggatcttga gtcggagctg agcttggagc
     2281 tgggacagga cttgcaggtt gactaggagc agttatggta tattgaaact agaatagtac
     2341 atatagactt ctaaaacatt gttagaattc gattttactg tcctgatcga tttgtcctgt
     2401 tcttattcca ttttactata ataaccgatg gtgtggttaa tgttggtaag agaaatttct
     2461 gaaaccatac ttcaaaaaag tcgctcgtca tcgtctcttc gtaagtcatt ggagcgatta
     2521 actcaccatt catttgttag acctgcaacc aaagaaattc tctgatatct tcttccagat
     2581 actttgcctc ttcttaactg accttttaat gagcgaccat attctcgata aaaataagta
     2641 tcgaatcctg tttcgtcaat ctaaacaggt gctaggtgct ttaaactatt aaaattctta
     2701 agaaataagg ctactttttc tgggtcttgt tcatagtagg tgtggttctt ttttcgagtg
     2761 tagcccatag ctttgagcgc atagtggatg gtagttggat gacagccaaa ttcagaagct
     2821 atttcagtca aataagcgtc
//
LOCUS       DAVLYR010000173         2838 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLYR010000173 DAVLYR010000000
VERSION     DAVLYR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.173"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..433
                     /locus_tag="VYO63_001841"
     CDS             <1..433
                     /locus_tag="VYO63_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672706.1"
                     /translation="KESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYSKDFRPLI
                     IDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIMESDGVNG
                     WIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1252..1611
                     /locus_tag="VYO63_001842"
     CDS             1252..1611
                     /locus_tag="VYO63_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672707.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1621..1986
                     /locus_tag="VYO63_001843"
     CDS             1621..1986
                     /locus_tag="VYO63_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1672708.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            1973..>2838
                     /locus_tag="VYO63_001844"
     CDS             1973..>2838
                     /locus_tag="VYO63_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1672709.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFTKQEIASRLEFLLNC
                     VNSLEGLLTKSK"
BASE COUNT          978 a          455 c          583 g          822 t
ORIGIN      
        1 gaaagaatct tcgcaacaac ttgatattct ctataaagaa gttggtgtat taagtttggg
       61 acaaaaagtt gtagccatgc ttgatttttt attagcatat agtgactatt ctaaagactt
      121 cagaccattg attattgatc agcctgaaga caatctagac aatcgttata tttacaggca
      181 tttagttcag cagtttagag atgtgaaagc tcaacgtcaa attattttag caacacataa
      241 tgctacaatt gtaacaaatt ctatgacaga tcaagttgtt attatggagt cagatggagt
      301 taacggatgg attgaatcac agggatatgt tagtgaaaaa tatataaaaa atcatatcat
      361 caatcaatta gagggaggaa aagattcctt caagcataaa atgtctatat atgagacggc
      421 tttatcagag tagagtcaga aaaagtaggt tagaaattta gcctactttt ttctttgttc
      481 gacaggcata gtgtacatct gaggtccaag tcctctgtgg atatttgctg cagatgaaac
      541 caatagcgac tcctaagcct gaatatcgtg aggtaggggg gataggaagg aattagcgaa
      601 atcaaggttc tacaaacaga atcgtgactt gaagccatat atagcggatg aggaactcta
      661 aaatccaaat aggtgtcgta acctatatac gtaaattacg agagtaaact aggaaagatg
      721 tacggcttat tccgtgagcg tttaggacgt agtacaacga atcatgggag tcagctgaac
      781 acatagtatt gaagaaattt ctgtaatgga aatggagcga agaagtgaac aattaaatga
      841 atacctctct aattaaattt gtcaattcta attcctggta tgaaaagaca gtgacctaaa
      901 aatgtaaacg atgggagctg atcataaata taggacggta catgcagtgg tgttagagat
      961 tagtccctac ttgatttgtg ataacttccc caactttctt ctgctatact tttctcaact
     1021 tttaaaaatc caactaagaa ttttacctgg gagtttgggg gcgtagccac caagttatct
     1081 tatggttagc tgtcaaaact ggaaggtttt ggttagctgg cgatatgatt ttttgggtat
     1141 tgtgaccaca atgcctgagc tcgcaaagac cgaacaagga ctttaagaaa tttcccgtat
     1201 gacacacggg gtcggggttt acccgagaaa ggatgaaagt gaggaaaaga gatgtctgaa
     1261 cagtaccgtg acattcgaaa agaggtaaat ctaaccgcag atgagctaaa acagattgaa
     1321 aaaatgatgg aggttgataa ttatcgtcac ttttcaccat tcgtcaggga taaaattttg
     1381 atgactgatg acaaacagtt agctgccaaa gaatggtttt ccctttggca gtctcaaaag
     1441 tttgaacaga tcagtcgaga tgtgcatttg gttttgatta tcgcaagaga gaatcaccaa
     1501 gtgactcaag aacacgtgtc aatcttattg acctgcgttc aagaattgat tgcggaagtc
     1561 aatcaagtac agtcactcag tcgtgggttt cgtgaaaaat acatgagata ggaggctact
     1621 atggtttatc gttatcgtac caatctcaaa aaagtatttc taacagattc agaattacac
     1681 caattgaatg aacggattgc taagagcaat tgtcaaaatt tttcagtcta tgccagaaaa
     1741 gttttgctta atcccaatat gtcatttgtc acgattaaca cggatactta tgaccagtta
     1801 gtttttgaat tgagacggat cggaaataac attaatcaaa ttgcgcgtgc tatcaatcaa
     1861 agccgtctga tttctcagga acagttacaa gaattgagta aaggagtcgg agagttgatc
     1921 aaggaagtgg acaaagaatt tcaagtggaa gtgaaaagat tgaaggagtt tcatggtagt
     1981 cactaagcat tttgcgacac acggaaaaaa atatcgtagg cgtttaatta agtatatcct
     2041 caatcctgat aaaacggaca atttgaaatt agtatctgat tttggtatga gcaattactt
     2101 agactttcct agctatgaag aaatggtaga aatgtacaat gtcaacttta ccaataatga
     2161 caagttgtac gaatacagaa atgaccgaca agaaaaacac caacaaaata ttcatgccca
     2221 tcacctcatt caatcatttt ctcctgaaga taatctgaca cctgaagaaa ttaaccgcat
     2281 tggttatgag accattatgg aattaacagg aggccgtttt cgtttcatcg tagcgactca
     2341 tacagacaaa gatcatattc ataatcacat cttaatcaac gccattgact gtaattcaga
     2401 taaaaagttg atatggaatt atgccttgga acgaaacctt cgtatgattt cagaccgcat
     2461 ttctaaaatg gctggggcga aaattattga aaagcgtttc tcttaccgtg actatcaaaa
     2521 atatagagcg actagccata agtttgaact aaagcaacgt ctttattttt tgatgcagca
     2581 gtcaaaatcc tttgatgatt ttttagaaaa agcagagcaa ttacatgttc atattgattt
     2641 tagtcagaag catagtcgat tcatgatgac ggatcgggca atgacaaaac caattcgagg
     2701 acgtcaactc agtaaacgag atttatatga tgaagatttt tttagaatgc attttaccaa
     2761 gcaagagatt gcaagtcgtt tagaattttt gttgaactgt gtcaattctt tagaagggtt
     2821 actaacaaaa tcaaaaga
//
LOCUS       DAVLYR010000174         2830 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLYR010000174 DAVLYR010000000
VERSION     DAVLYR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.174"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..143)
                     /locus_tag="VYO63_001845"
     CDS             complement(<1..143)
                     /locus_tag="VYO63_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1672710.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     GKEA"
     gene            348..536
                     /locus_tag="VYO63_001846"
     CDS             348..536
                     /locus_tag="VYO63_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1672711.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            693..1058
                     /locus_tag="VYO63_001847"
     CDS             693..1058
                     /locus_tag="VYO63_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1672712.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            1061..2728
                     /locus_tag="VYO63_001848"
     CDS             1061..2728
                     /locus_tag="VYO63_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1672713.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT          901 a          516 c          624 g          789 t
ORIGIN      
        1 gcttcttttc ctttctgttg ataggctagt agcagacgat tgatttgtct ttcagaaaga
       61 ttgagttcga cacaggcccg tttctttgtt ttctttcctt gggctatagc ttttatcaca
      121 agatattttt tcgtttcatt catattcagt tggatccttt tcatatgact attctaccaa
      181 atgtgacatt ttcacgttcg atttactaaa gacattatca cattcgaatc acaacacaaa
      241 taaaaaatct tccaattccc ttgcaaaaaa caagaaagtg tgataacata gtacggtatg
      301 tggtgctagc acatccgcta tattagatct aataggagga aaacacaatg gctaaagtat
      361 gttactttac aggtcgtaag actgtatcag gaaacaaccg ttcacacgcg atgaaccaaa
      421 caaaacgtgc cgtaaaacca aaccttcaaa aagttactgt tcttatcgat ggtaaaccta
      481 aaaaagtttg ggcttcagct cgtgctttga aatcaggtaa agttgaacgc gtttaataat
      541 aaaagcaaag gagaccttag ggtctttttt cttttattgt gggtataaca tcgtttgaaa
      601 aatagagtaa atatccgctg atacatcatt ctgcttttac acttgggctg aaatatgata
      661 aaatagagta tcaactagtt gaggtaaaaa ggatgactgt aaaaattaat acaaaagatg
      721 gtcaaatcga actaacagat gaagtgattg caaccgtcgt aggtggagca gcaactgaga
      781 tttttggtgt ggtcggtatg gctagtaaaa atgccctcaa agataatttc caagccctgc
      841 taggtaagga aaattattct aaaggtgtcg tcgtaaaggc agccgaagat ggcagtattg
      901 cagttgatgt atataccgtg ttaagctacg gaacaaagat tagtgaagtg tcaaaaaaca
      961 ttcaagagcg tgttgccttt agcttggaaa accagcttgg aattactgct cagactgtaa
     1021 atgtctacat tcaaaatatc aaagttgtag gagaataacc gtgtcaaaga ttactactag
     1081 cttatttcaa gaaatggtgc aggctgcatc aactcgcttg aataagcaag ctgaatatgt
     1141 caattcatta aacgtctttc cagttccaga tggagatact gggacaaata tgggaatgac
     1201 cattgaaaat ggtgctaaag aagttgcaga caagccagct tctacagttg gagaagtagc
     1261 gagcattctt gccaaagggc ttttgatggg tgcgcgtggg aactcaggag tgattacatc
     1321 tcagcttttc cgtggatttt cccaagctat caaggataaa gacgagttaa caggtcaaga
     1381 cttggctctg gccttccaat caggtgtgga agttgcctat aaggcagtga tgaaacctgt
     1441 tgaaggaacg attttgacag tttctcgtgg agctgctatc ggtgctaaga aaaaagctga
     1501 gcaaacagat gatactgttg aagtcatgcg cgcagccttg gaaggtgcta aaacagctct
     1561 agctaaaaca ccagacatgc ttccagtatt gaaagaagtt ggcgttgtgg actcaggtgg
     1621 tcaaggattg gtcttcatct acgaaggttt cctttcagcc cttactggcg aatatattgc
     1681 atctgaggac tttgtagcga ctcctgccaa catgagtgag atgatcaatg tagagcatca
     1741 taagtctgta gctggtcacg tagcgactga ggacatcacg tttggttact gtactgaaat
     1801 catggtagct cttaagcaag gtccaaccta tgctaaagat tttgactacg atgaattccg
     1861 taactacttg gatgagcttg gggattctct cctcgttgtt aacgatgatg aaattgtcaa
     1921 agtccatgtt catacagaag atccaggact tgttatgcaa gaaggtctca aatatggtag
     1981 cttggtcaag gtaaaagttg acaatatgcg taaccaacac gaagcacagg ttgagaaaga
     2041 agctactcaa gttatcaagt cggctgaaga aaaagagtat gctttgattg ctgtggtggc
     2101 tggtaaaggt ctagcagata tcttctgttc tcaaggcgtg gattatgtta tcgaaggcgg
     2161 tcaaaccatg aacccttcaa cagaagactt tatcaaggct gttgaacagg tcaatgcccg
     2221 taacatcatc ttcttgccaa acaacaagaa catcttcatg gcagctcaat ctgcggcaga
     2281 agttttggag caaccagcag tagtggtaga ggctcgcact cttcctcaag gtatgacaag
     2341 tcttcttgcc tttgatccaa gcaagtccat tgaagaaaac caagagcgta tgacagctgc
     2401 tcttagcgat gtcgttagcg gaagcgtcac aacagccgtg cgtgatacaa cgatcgatgg
     2461 cttagaaatc catgaaaacg ataatctagg tatggtggat ggaaaaattc ttgtgtcaaa
     2521 ccctgatatg caccaaacat tgactgaaac cttgaaacat atgttggatg aagacagtga
     2581 aatcgtaacc ttctatgtcg gtgaagacgg aagcgaagaa cttgccaatg aaatcgctca
     2641 agaaatcgta gaagaattcg aagacgttga agtcgagatt caccaaggtc aacaacctgt
     2701 ttacccatac ctatttagtg tggaataaga ttaatgaagg actgagaaat cagttctttt
     2761 ttctttttta gtaaatgaaa tcggtatctt tttaataaaa acaaaaataa cattcataaa
     2821 taaacgttaa
//
LOCUS       DAVLYR010000175         2728 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLYR010000175 DAVLYR010000000
VERSION     DAVLYR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.175"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..127)
                     /locus_tag="VYO63_001849"
     CDS             complement(<1..127)
                     /locus_tag="VYO63_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEV1672714.1"
                     /translation="MENFGAVLKDIRISKNFRLKDLSCNEISESTISRFENGITKL"
     gene            221..1441
                     /locus_tag="VYO63_001850"
     CDS             221..1441
                     /locus_tag="VYO63_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEV1672715.1"
                     /translation="MKKILLSTVALLSLVASLLANNPVSAQESSSQATYSKSSGSWIK
                     SGNRWWYKHSDGSYTTNGWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTG
                     WCLVSGSWYYLNSSGVMQTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQ
                     NINRRALVLGETSTRAVPIADVNAMEKVFNNQNFSEVVRFPDRTKSEIIAKMQELFES
                     SSEGDVNYLYFTCHGGRDGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTI
                     ISKDNTGEGNEGASTEYFDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSG
                     GSLSLATKYWSLGSGWNPLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHI
                     EVYPDNSQFVLFKK"
     gene            1454..2635
                     /locus_tag="VYO63_001851"
     CDS             1454..2635
                     /locus_tag="VYO63_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1672716.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREKGTLNLIKPGRIARQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYFMDDEGVMFNQTWLHQGGSWYAFKSSGAMISA
                     DWLYDNGSWYYLKDSGSMVTGWLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSM
                     ATGWVKDSGSWYYLKNSGSMATGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAW
                     K"
BASE COUNT          912 a          376 c          589 g          851 t
ORIGIN      
        1 ataattttgt aatgccattt tcgaaacgag aaatagttga ttcactaatt tcattacatg
       61 ataaatcttt aaggcgaaaa ttttttgaaa tacgaatatc ctttaaaact gctccgaaat
      121 tttccataat gttcctcttc catttctgca aaaaataaaa cgcttgtttt ttatttatga
      181 tatgattata caaaaataaa atagaaagga caataatatc atgaaaaaaa ttttattgtc
      241 aacagttgct ttattaagct tagtagcatc tctgctagca aataatccag tttctgctca
      301 agaatcatcc tctcaagcaa cttatagtaa gtcaagtgga agttggataa aatctggtaa
      361 ccgttggtgg tataagcatt ctgatggttc ttatactaca aatggatggg aaaaaataaa
      421 tggtacctgg tactattttg atagtgaagg ttggatgaag acaggttgga ttaaagaata
      481 tgggaaatgg tattacctag atgatagtgg tgctatgaaa actggatggt gtttggtttc
      541 tggtagctgg tattatttaa atagtagcgg tgtaatgcaa acgggactac agactatcaa
      601 tggaaaacaa tattatttag ctgccggcgg tgctatgcag acaggttggc acaatatcgg
      661 agatgatacc tatttctttg ctaatagtgg ggaaaatcaa aatatcaatc gtcgtgcttt
      721 agttcttggt gaaacatcaa cgagagcagt acctattgca gatgttaatg ctatggaaaa
      781 ggtttttaat aaccaaaact ttagtgaagt tgttcgtttc ccagatagaa caaaatccga
      841 gataattgca aaaatgcaag agctttttga atcttctagc gaaggcgatg tgaactatct
      901 ttactttact tgtcatggag gaagagatgg aagaatctat attggtagtg atggcctagc
      961 attttctggt tgggaattgg cgtccgtttt gaaacaatac aaaggtaagt ttgttgttat
     1021 gctggattgt tgtcatgctg gaacaattat ttctaaagat aatacaggtg aaggcaatga
     1081 aggagcctct acggaatatt ttgatttaga tgaatttgta tcaggatttt ctaatatgga
     1141 tggtaacgaa aaatcaggtg agatgattga ctctaaattt ttagtattat gctcatcaag
     1201 aggtgcagaa tactctagtg gtggatcatt aagtttagcg actaaatatt ggtctttagg
     1261 ttcaggatgg aatcctctac agaattcaca agcatattta gcagcagacc aaaataataa
     1321 tcgcagaatc acattaaatg aattgtatac atactctcgt gaacaagttt tgaaacaaaa
     1381 ttctaaccag catattgaag tttatcctga caacagtcaa tttgtgttat ttaagaaata
     1441 agggggcaat aaaatgaaag tatcaaaaaa aattacacta tttagtttgt cttttgcagg
     1501 ttttgtttta ttgactttac ctcaagcagg aaaggctttt gaacttaaag aagactgggc
     1561 atttaaaggt ggcattcgat acgagaatgg gaaagtcagc aaaattaata atggatatga
     1621 agtaaatatt aaagtgttag atttacctag tactagcgca atcgaatgga cagttagatt
     1681 gaatggagaa aagcaaaata ctaacttctt agcggaggaa agaactgtat ctaaaactga
     1741 agataaggga cgtttcttgc acttttatat cccctatgga tatcgtgggg atattgtagt
     1801 agaggctaag agtggaaacg aagtgaagac ttggtctact aaggtagttg acgatgttta
     1861 ttcagattct gctaagagtg gctactttat tctcgatggg gaacaaatct tagaaagttc
     1921 atgggattcc gtaaatgagt cttatattgc aacgcttcca actgtaacat caggaaaaac
     1981 tgttgttgct tggcgtgaaa aaggaactct taatttaatt aaaccaggaa gaattgctcg
     2041 gcaatataat agcagtggat catatgtaga actttctcca atctttgaaa ctgctagttg
     2101 gctaaaatca aaccaaaact ggtactatca aaaacaaggt caattagttc aaaattcttg
     2161 gatcaaggat cagggaagct ggtactttat ggatgatgaa ggggttatgt ttaatcaaac
     2221 atggctacac caaggtggaa gctggtatgc ctttaagtca tcaggagcca tgatttcagc
     2281 tgattggttg tatgataatg gttcttggta ttacctaaag gactcaggtt ctatggtaac
     2341 aggatggctt aaaaatggag gctcttggta ttacctaaac aagtcaggtt caatggctac
     2401 aggttggatt aaagatagtg gaacttggta ttatctaaag aattcaggct ctatggcgac
     2461 aggctgggtg aaagatagtg gctcttggta ttatctaaag aactcaggtt caatggcaac
     2521 aggttgggtc aaagataatg gtaagtggta ctatctcgca tcatctggta atatgcttcg
     2581 caatacacgc acaccagatg gctattatgt agatggcagt ggcgcttgga aataaatttt
     2641 taaaagagaa aatctactga gaaggtagat tttcttttta gttaatgaat atagaataaa
     2701 gtagatcagg actggtctat tttatttt
//
LOCUS       DAVLYR010000176         2696 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLYR010000176 DAVLYR010000000
VERSION     DAVLYR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.176"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            136..942
                     /locus_tag="VYO63_001852"
     CDS             136..942
                     /locus_tag="VYO63_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1672717.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(1108..1644)
                     /locus_tag="VYO63_001853"
     CDS             complement(1108..1644)
                     /locus_tag="VYO63_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1672718.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FIGQEVVQFYLDLLIPKN"
     gene            1777..2667
                     /locus_tag="VYO63_001854"
     CDS             1777..2667
                     /locus_tag="VYO63_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1672719.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
BASE COUNT          761 a          437 c          640 g          858 t
ORIGIN      
        1 tacttaacaa ataagaggtt ttagctcatg tcgactaagg ttagaaatct tgtgtatagg
       61 cagttcatta tctaatagag cgttgcgtcc gaaagtctat ccagacacgg ctctttaaaa
      121 acaaaaggag aaatgatgca tacttatttg caaaagaaaa ttgaaaatat caaaacaacc
      181 ctaggtgaaa tgtcaggtgg ttaccgtcgt atggttgcgg ctatggctga tttaggattt
      241 tcaggaacta tgaaggctat ctgggatgac ctctttgccc atcgtagttt tgcccagtgg
      301 atttatttgc tggttttagg aagttttcct ctctggctgg agttggttta cgaacatcgt
      361 attgttgact ggattgggat gatttgtagc ttgacaggga ttatctgtgt aatctttgta
      421 tcggaaggtc gagcaagtaa ttatcttttt ggcttgatta actctgttat ttaccttatt
      481 ttggccctac agaaaggctt ttatggtgag gtgctgacga cactttactt cacagtcatg
      541 cagccaattg gacttctagt ttggatttat caggcacagt ttaagaagga aaagcaggag
      601 tttgtcgcgc gtaaactgga cggcaagggc tggacaaagt atctttccat tagtgtgctt
      661 tggtggttgg cctttggctt catttatcag tctattggtg ccaatcgtcc ctatcgtgat
      721 tcaatcacag atgcaaccaa tggggtaggg caaatcctca tgacagctgt ttaccgtgaa
      781 cagtggatat tctgggcggc taccaatgtt ttttcaatct atctctggtg gggagaaagc
      841 ctgcaaattc aagggaaata tctaatttat ctcattaaca gtctagttgg ttggtatcaa
      901 tggagcaagg cagctaagca gaatactgat ttacttaact aggaaaagat gtttgaaagt
      961 gctgttttga gatttcgatt aaaacagata tagttgataa tcaaggattt atagtatgaa
     1021 aaagaggatc ggcgggtcct cttttgttgt tgaaaagata aaaaactcag taacctagaa
     1081 ataagacaac tgagctttac tctatattca atttttagga atgagaaggt ctagataaaa
     1141 ttggacaact tcctggccta tgaaatcttg accttttttg agccaccagg tcaatgtctc
     1201 gataaagttg gacacgacca agtgttggag gtaagaagta ggcatattag ggtgggcttc
     1261 ttttaaatta tcagctagca cggaatagac atggtgttct agctctttat ggagttgacg
     1321 gaggaagtag tcatttttgg aaaatagcag actggtgata tggtcttggt ttttctgaaa
     1381 atggagaaag aggtgggcga ggtagtcctc ggttgaaatg gcttgctctc tttcaaaaag
     1441 atgatggaag aggtagcggc agagctggtc cagaagaagc tccttacttt catagtgaca
     1501 gtaaaaggtg gatcttccca catctgcgag atcaatgata tcctgaacag tagtggcctc
     1561 gtagccctta gcattcaaaa gttgtataaa agcttgatag atggcttttt tggttttgct
     1621 gatacggcgg tcaatgttag tcatatggac acttaaggca aattgttcag aactgaataa
     1681 agctgacgtt ttgcttctat cctttctttg agttttagtg gataatgata atgaacaagg
     1741 tgttcataaa tctattataa caaaggaatg agaaatatga aggcaaaata tgctgtttgg
     1801 gtggcttttt tcttaaattt gacttatgcc attgttgagt ttattgcagg tggagtattt
     1861 ggttctagcg ctgttcttgc tgactctgtg catgacttgg gagatgcgat tgcaattgga
     1921 atatcagctt ttctagaaac aatctccaat cgtgaagaag acaatcagta caccttgggc
     1981 tataagcggt ttagcctgct aggagccttg gtaacagctg tgattctcgt aacgggctct
     2041 gttctagtca ttttggaaaa tgtcacgaag attttgcatc cgcaaccagt caatgatgag
     2101 gggattctct ggttaggaat tattgcgatt actatcaatc tgttagcgag tctggtggtt
     2161 ggtaagggaa agacaaagaa tgagtctatt ctgagtttgc attttctgga agatacgcta
     2221 gggtgggtag ctgttatcct gatggcgatt gttcttcgat ttacggactg gtatatccta
     2281 gatcctcttt tgtcccttgt catttctttc tttattcttt caaaagccct tccacgtttt
     2341 tggtctacac tcaagatttt cttggatgct gtgccagaag gtcttgatat caagcaagta
     2401 aagagtggcc tggagcgatt ggacaatgtg gccagcctta atcagcttaa tctctggact
     2461 atggatgctt tggaaaaaaa tgccattgta catgtttgtc taaaagaaat ggaacatatg
     2521 gaaacttgta aagagtctat tcgaattttc ctaaaagatt gtggttttca aaatattacc
     2581 attgaaattg atgctgacct agaaactcac caaacccata agcgaaaggt gtgtgacttg
     2641 gaacggagtt atgagcatca acattagaaa aaagtgaaaa atacttgggt actatc
//
LOCUS       DAVLYR010000177         2687 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLYR010000177 DAVLYR010000000
VERSION     DAVLYR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.177"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(143..757)
                     /locus_tag="VYO63_001855"
     CDS             complement(143..757)
                     /locus_tag="VYO63_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1672720.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIQRL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1572..1841)
                     /gene="rpsO"
                     /locus_tag="VYO63_001856"
     CDS             complement(1572..1841)
                     /gene="rpsO"
                     /locus_tag="VYO63_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1672721.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1982..2611)
                     /locus_tag="VYO63_001857"
     CDS             complement(1982..2611)
                     /locus_tag="VYO63_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1672722.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADEFDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
BASE COUNT          843 a          535 c          455 g          854 t
ORIGIN      
        1 tataagaatc ccctaatcaa atcaaaagcc actctacaat agagtaactt taagattgtc
       61 catttatact caatgaaaaa gccctctgaa aatcagagag ctgatttgag ctcaggctaa
      121 aatcctagtg aaacaaaaaa atctacacga tcagaaaagt ctctatcgtg ccattttcat
      181 acatgatgta tagtccaagt agaatgaata ctaagggcac aatgattcgc tcgtattttt
      241 caattgtctc gaatattaac ggaatagagg ataacaccct actaatctcg caaaagataa
      301 ttatgccgat tacaaacaca agcaaggcca cgagggtctg tgaccaatct aacgaagcaa
      361 aataaggtat atagatacct aaattatctc cgccagacgc aattgtcagc aatgtaactg
      421 tccaaaacag ttgatttgcc ttgctttgtt ctaatctttg aataatttct tcctcttctt
      481 cttccgcatc ttctccaaca attgcaaagc gaatccctaa atagataggg attaaaccaa
      541 gcaatccaac catccattct tcaggcacga aattaacgac ataagcagca actaaactcg
      601 cccctacaag taagcctgtg cctagatatt gccccgcata aatatgccat ttctgtttat
      661 tctgtgatag ctgtgcaaat aaaataatta aaataattaa ataatcgata ctggtggaaa
      721 tataaacacc aatagcagat atgattgtct gtcccataaa aaaaacctcc tgtattagtc
      781 agtaataaat cagcagattt taggagagca caaacacatt aatttagcta tcgctagtgg
      841 gtaatgtcga acgtaggaaa gcattttact cctcctcaaa acgtagttac aaagtaattt
      901 ctaactggaa ttcagtgact acttccatgt aaagtataac atactatact ttactttgta
      961 aagagtgggc tcttctaggg ggcttgcata cagctactcg gcttgaaaag ccgagtagct
     1021 gcgcaccttt atggtgtaat tagctgatac catttgagat ttttgtagtc tccaaaattg
     1081 ttaccgataa aacctcaata aaaaagccct ctgaagtcag agagctgatt ttagcccggg
     1141 ctaaaatcct agtgaaaaag atgaaactcc ttgtgctcat cgaacactgt gtcatttccc
     1201 tattttcata cggatttttg acacccttag catcatgatt cttgctagat aaatcgttta
     1261 tagactaggg gacgaggtga agatcgtacg aaactttcag tgaaacaaaa aaatctccac
     1321 gaagggagaa aagctagttt attttacctt acagtgctag gaaccgtaac cgaagattat
     1381 acttgagtat agcgaggtaa ggtgacaacg caacaaagct acccaattta gagtagcttt
     1441 attatcaaga tatgctcaat tgaacacggc ctaagcactt ggcaaaaaag ataaaatctc
     1501 ctagaaactg aagtttcttc gtcaattttc ctatttttgc tttgtgcttt tgacggcctt
     1561 tgtatcttga attagcgacg aagtcctaga gagttgatca actcacggta acggttaacg
     1621 tcgtttttac gcaagtatgc aagcaagtta cgacggcgac cgattttttt catcaatcca
     1681 cggtaagtag cgtggtcttt tttgtgttgt ttgatgtgtt cgttaaggtg gttgatttcc
     1741 caagtaagga cagcaacttg aacctctact gaacctgtat caccttcgtg acgtgcatat
     1801 tgtgcaatga tttcattttt tttctctttt gagattgcca tgatgtttct cctttttatt
     1861 tggcttcatc cgagtgacag gttggcatgc ctgtaaccaa gaagaagtta tttgtcttta
     1921 cgacaatccc tattttacca agatttaaat tttctgtcaa acattaatac tctcatagtt
     1981 attatttttc aagtaaagag atacgttctt ttatatgatg acctttttca agttcatcta
     2041 ccaatccaat agcataatct gcataactaa tctggcttat tccttttcca ttagtagtaa
     2101 aaatttctcc tgctagaatg tagtttcctg ttttttcacc atcgggaata aaatctacag
     2161 caggactgac aaaagtccag ttgagattat tttttgtacg aagaagatca aattcatccg
     2221 cttgagcttt tgctagtgac ttaaattctt caggaaaatc agacgtatct aaaagtcgtg
     2281 tcgtttttgt ttcatctata taaagacttc ctgctccccc tacaactaga aaacgtgtct
     2341 gtgttccagc aagaagctga ttaaatagtt cgattgattt gctgtggagc ggtagcgtat
     2401 ctggtgtata agcaccaaac gctgaaataa cagcatcaaa tccagtaaga tcatcttttg
     2461 tcaactcaaa taaatctttt ttaataatag actcagcttg acttttgttt tcagaacgaa
     2521 caatagccgt tacttcatgt cctcgtttga ctgcttcttc aacaattgct ttccccgctt
     2581 gtccatttgc tgcaataact gctagtttca ttttttatac ctctattgtt gtaattattt
     2641 tagttacaac aaatgtgaca ctcttaataa tcaatatcaa tagtctt
//
LOCUS       DAVLYR010000178         2678 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLYR010000178 DAVLYR010000000
VERSION     DAVLYR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.178"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(109..567)
                     /locus_tag="VYO63_001858"
     CDS             complement(109..567)
                     /locus_tag="VYO63_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1672723.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(564..1004)
                     /locus_tag="VYO63_001859"
     CDS             complement(564..1004)
                     /locus_tag="VYO63_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672724.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1437..2303
                     /locus_tag="VYO63_001860"
     CDS             1437..2303
                     /locus_tag="VYO63_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1672725.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
BASE COUNT          889 a          384 c          575 g          830 t
ORIGIN      
        1 tgtcctgatc gatttatcct gttcttattt catttcacta tagtaaggtg cagcaggata
       61 aagaaaaacc gagaagtttc tttctcggat ttgcttatta tgaggttcct attttctatg
      121 ctccaagagt tgattgatgt gatctacttt tttagcctct cttttataga gaatagccca
      181 gatgatgaga tagacaatcg caaactcgat aatgagctgg aggtagaaaa tccagtggaa
      241 agggaaccaa ccagcaagag ttgctagtgg gacaaagcca gccagcataa ggaagaaatg
      301 agtcagagtg gcacgaagca tgctccagtc acggctaaat aagcggttgc caaagttgaa
      361 gagcataccg atggttgccc agataagtgt gcagtagagc aagaccaggg caccgtgaac
      421 ctgatgctga tccatcactt ggccgatgag agagtaggga tttagtggtg cgtaggtatt
      481 tggtgcataa atgagtgaaa agaggataga gaggatgagg ccgataagaa caccggtagc
      541 tgcatcgtga aatacttgtt ttttcatagt tctaatttct ccttgatggt ttttagataa
      601 cggcgtgaag agtaggtgaa gctttcgttt ttcaagaaaa tttctaccag accgtttggc
      661 gtgagcttga ggtgagagat ggaatcgata ttgatgattt ctgattggga aatttggata
      721 aaattggttg gcaagagttt aagaacctga tagagtcgca aatcaatgct gtaggtctga
      781 ctcgcggttt ctgctagaac cttccgattc tcgatataga agcgctgaat cttgccaatc
      841 tcaactagat agacctgatc atcgattttt cctttgattt tttctctttg gtccagattt
      901 tctgcgaact cgatgacttt ctggactttt tcggtttctt gaggtgcttg gacaatcagc
      961 ttttcctcct cgtaagtctc actaatctgt agttctactt tcatagtttt ctctcctttt
     1021 cagttataca aggttgtgat cacttcctgt atatccttat taaacactat tttatagccc
     1081 atggcaagga accttgtcta tgtggaggga ttgggctcct atgtggtgga gcttttctgt
     1141 tctttctgaa atatggtata atagcactaa tcaatttcta ggaaaataga tacggaaagg
     1201 ggctgaaaga actaggggaa cagttggaat tagaaagtga taggtatgaa aagtggcgtt
     1261 gaagctgaat aagtttataa agcgtttgaa tgaagaaatg gaaagtaaat aagttgacga
     1321 tgaattacaa aatagatttt atctacaatt tgtatctttc aaatcattga tttgaatttt
     1381 agatttaatc atgttacaat taaaactgaa atcaaataga agaggaaaat gcaaacgtgg
     1441 gcacactatt agcaacaaga ttaaaaaata gacggaaaga attaaaaatg tctcagcgag
     1501 aactagctga aggtatctgt aaacaggggc aaatcagtcg attagagagc ggagagttta
     1561 cccctggagc ggatttcctg aatgcccttg ctaagaagtt aaaagtcagt atggattatt
     1621 tttttgatga gcaggttgta gagaaagttg aggagttatc agaatttaag aaattagcac
     1681 agacatttat tacaaatcgc aattatgagt ctttgaaata tatatatgaa ttggagaatg
     1741 taaaagctca ccgcctatct ttagcagata aaatctacat ggaatggata aaatctctga
     1801 tagattttta tttttacgga cagaaagaag atgcgattgt acgattagag ggggtattat
     1861 cacagttaag tgtatctgat ttgaactatc tccaagtttc aaacacccta ttcaattttt
     1921 attatgatat tggagattta ggtcgtttca ataaaattcg tgaaaaacta gagtatcaag
     1981 taaatcacct caatttgagt acaatcgaag agttagaact atctattaag tttaactaca
     2041 acgtttgtcg ttatctatgg ttacagaaaa atattgaaga agctattact aaaattacag
     2101 ctactataaa acaatgtaag gcttacagaa cgaattatct tttggctgat ttgtacttat
     2161 tgatgggaaa tgtaagtgaa aatttttctt ctaaaagttc ggtgaaagaa tattttgaga
     2221 cggcatattt tctatacaaa cttgaggaga acatgtcaat gactctgaaa gttgagcatt
     2281 acattgcaga tataacagaa taatgaatac ggtgttcaag tgagcatcgt ttttcaaacc
     2341 tagttcagat acaaaaaagt atcaaacaaa aaaaaataaa aaaacattat aaaaacaatt
     2401 gacaagctaa ttttgaagtg ataaaataaa tgcaagaaaa caaaaggaga ggtatatgag
     2461 aagtttgttt aagaaaatag ttgctgtatt agttattggc ttaattctgt taggaattgc
     2521 gagaacacct caagttcata agatggctag agggattgat cctggtcctg cgaattttat
     2581 ttagaaatca atttctacta attgagtact aattgagtat taatgggtaa aaagattgta
     2641 tggataagaa aaatttactt gtattggtta ctggttcg
//
LOCUS       DAVLYR010000179         2562 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLYR010000179 DAVLYR010000000
VERSION     DAVLYR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.179"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            107..2431
                     /gene="pflB"
                     /locus_tag="VYO63_001861"
     CDS             107..2431
                     /gene="pflB"
                     /locus_tag="VYO63_001861"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1672726.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT          805 a          545 c          508 g          704 t
ORIGIN      
        1 agtgtaaaaa tagggaattt tcacttgttg aaatcggtta ctatatggta tacttgtctt
       61 atgaatgtaa cagatgactg ttactagaaa aaaagaggac attaatatgg ttgttaagac
      121 agttgttgaa gcacaagata tttttgacaa agcttgggaa ggcttcaaag gcgtagattg
      181 gaaagaaaaa gcaagtgtat cacgatttgt acaagctaac tacacacctt atgatggaga
      241 cgaaagcttc cttgcaggac caacagagcg ttcacttcac atcaagaaaa ttgtagaaga
      301 aactaaagca cactacgaag aaactcgttt cccaatggac actcgtccaa catctatcgc
      361 tgatatccct gctggattta tcgacaaaga aaatgaagtt atctttggta tccaaaatga
      421 tgaactcttc aaattgaact tcatgccaaa aggtggtatc cgtatggctg aaactacttt
      481 aaaagaaaat ggatacgaac cagacccagc tgttcacgaa atcttcacta aatatgtaac
      541 aacagttaac gacggtattt tccgtgccta cacttcaaat attcgtcgcg ctcgtcatgc
      601 acacactgta actggtcttc cagatgcata ctcacgcgga cgtatcatcg gtgtttacgc
      661 acgtcttgct ctttacggtg cagactactt gatgcaagaa aaagtaaacg actggaatgc
      721 aatcaaagaa atcgatgaag aaacaatccg tcttcgtgaa gaagtaaacc ttcaatacca
      781 agcattgcaa caagttgttc gcctgggtga cctttacggg gttgatgttc gcaaaccagc
      841 gatgaacgtg aaagaagcaa tccaatgggt taacattgct ttcatggctg tctgccgtgt
      901 gattaacggt gctgctacat ctctaggtcg tgtaccaatc gtattggaca tctttgcaga
      961 acgtgacctt gctcgtggta catttactga atcagaaatc caagaattcg ttgatgattt
     1021 cgttatgaaa cttcgtacag ttaaatttgc tcgtacaaaa gcttatgacc aattgtactc
     1081 aggtgaccca acctttatca caacttctat ggctggtatg ggtaacgacg gtcgtcaccg
     1141 tgttactaag atggactacc gtttcttgaa cacacttgac aacatcggta actcaccaga
     1201 accaaacttg acagttcttt ggactgacaa attgccatac aacttccgtc gctactgtat
     1261 gcacatgagc cacaaacact cttctatcca atacgaaggt gtaacaacaa tggctaaaga
     1321 cggatatggt gaaatgagct gtatctcatg ctgtgtgtct ccacttgatc cagaaaatga
     1381 agaacaacgc cacaacatcc agtacttcgg tgctcgtgta aacgttctta aagcccttct
     1441 tactggtttg aatggtggtt acgacgatgt tcacaaagac tacaaagtat ttgatatcga
     1501 accaatccgt gacgaagttc ttgaattcga atcagttaaa gcgaactttg aaaaatctct
     1561 tgactggttg actgacactt acgtagatgc cttgaacatc atccactaca tgactgatag
     1621 atacaactac gaagctgttc aaatggcctt cttgccaact aaacaacgtg ccaacatggg
     1681 attcggtatc tgtggatttg ctaacactgt tgatacattg tcagctatca aatacgctac
     1741 agttaaacca atccgtgacg aagatggcta catctacgat tacgaaacaa tcggtgacta
     1801 cccacgctgg ggtgaagatg acccacgttc aaacgaattg gcagaatggt tgatcgaagc
     1861 ttacacaact cgtctacgta gccacaaact atacaaagac gcagaagcta cagtatcact
     1921 tttgacaatc acatctaacg ttgcttactc taaacaaact ggtaactcac cagttcacaa
     1981 aggtgtatac ctcaacgaag atggttctgt gaacttgtct aaacttgaat tcttctcacc
     2041 aggtgctaac ccatctaaca aagctaaagg tggttggttg caaaacttga actcactttc
     2101 tagccttgac tttagttatg cagctgacgg tatctcattg actacacaag tatcacctcg
     2161 cgctcttggt aagactcgtg atgaacaagt tgataacttg gtaacaatcc ttgatggtta
     2221 cttcgaaaac ggtggacaac acgttaactt gaacgttatg gacttgaacg atgtttacga
     2281 aaaaatcatg tcaggcgaag acgttatcgt acgtatctct ggatactgtg taaacactaa
     2341 atacctcact ccagaacaaa aaactgaatt gacacaacgt gtcttccacg aagttctttc
     2401 aatggatgac gccttggatg cattgagcta atcaagttcg gctctttgtc aactgcagtg
     2461 ggttgaagtc agctaagctc gagaaaggac aaaattcgtc ctttcttttt tgatgttcag
     2521 agcgataaaa atcctttttt tgaagttttc aaagttccga aa
//
LOCUS       DAVLYR010000180         2466 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLYR010000180 DAVLYR010000000
VERSION     DAVLYR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.180"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            62..>2466
                     /locus_tag="VYO63_001862"
     CDS             62..>2466
                     /locus_tag="VYO63_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1672727.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEG
                     DAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIY
                     NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTL
                     EDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKPE
                     EDKEHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQV
                     EHSV"
BASE COUNT          841 a          491 c          523 g          611 t
ORIGIN      
        1 ggagtttctg caatttagaa atgaattgca actagaaata tcaaatagaa agagagtttc
       61 gatgaaaatt aataagaaat accttgttgg ttctgcggca gctttgattt taagtgtttg
      121 ttcttacgag ttgggactgt atcaagctag aacggttaag gaaaataatc gtgtttccta
      181 tatagatgga aaacaagcga cgcaaaaaac ggagaatttg actcctgatg aggttagcaa
      241 gcgtgaagga atcaatgctg agcaaatcgt catcaagata acagaccaag gctatgtcac
      301 ttcacatggc gaccactatc attattacaa tggtaaggtt ccttatgatg cgattttcag
      361 tgaagaatta ctcatgaaag atccaaacta taagctaaaa gatgaggata ttgttaatga
      421 ggtcaagggt ggatatgtta tcaaggtaga tggaaaatac tatgtttacc ttaaggatgc
      481 tgcccacgcg gataacgtcc gtacaaaaga ggaaatcaat cgacaaaaac aagaacatag
      541 tcaacatcgt gaaggtggga ctccaagaaa cgatggtgct gttgccttgg cacgttcgca
      601 aggacgctat actacagatg atggttatat ctttaatgct tctgatatca tagaggatac
      661 tggtgatgct tatatcgttc ctcatggaga tcattaccat tacattccta agaatgagtt
      721 atcagctagc gagttagctg ctgcagaagc cttcctatct ggtcggggaa atctgtcaaa
      781 ttcaagaacc tatcgccgac aaaatagcga taacacttca agaacaaact ggctaccttc
      841 tgtaagcaat ccaggaacta caaatactaa cacaagcaac aacagcaaca ctaacagtca
      901 agcaagtcaa agtaatgaca ttgatagtct cttgaaacag ctctacaaac tgcctttgag
      961 tcaacgccat gtagaatctg atggccttgt tttcgaccca gcgcaaatca caagtcgaac
     1021 cgccagaggt gtagctgtcc ctcatggtaa ccattaccac tttatccctt atgaacaaat
     1081 gtctgaattg gaaaaacgaa ttgctcgtat tattcccctt cgttatcgtt caaaccattg
     1141 ggtaccagat tcaagaccag aacaaccaag tccacaatcg actccggaac ctagtccaag
     1201 tctgcaacct gcaccaaatc ctcaaccagc tccaagcaat ccaattgatg agaaattggt
     1261 caaagaagct gttcgaaaag taggcgatgg ttatgtcttt gaggagaatg gagtttctcg
     1321 ttatatccca gccaaggatc tttcagcaga aacagcagca ggcattgata gcaaactggc
     1381 caagcaggaa agtttatcgc ataagctagg agctaagaaa actgacctcc catctagtga
     1441 tagagaattt tacaataaga cttatgactt actagcaaga attcaccaag atttacttga
     1501 taataaaggt cgacaagttg attttgaggc tttggataac ctgttggaac gactcaagga
     1561 tgtctcaagt gataaagtca agttagtgga tgatattctt gccttcttag ctccgattcg
     1621 tcatccagaa cgtttaggaa aaccaaatgc gcaaattacc tacactgatg atgagattca
     1681 agtagccaag ttggcaggca agtacacaac agaagacggt tatatctttg atcctcgtga
     1741 tataaccagt gatgaggggg atgcctatgt aactccacat atgacccata gccactggat
     1801 taaaaaagat agtttgtctg aagctgagag agcggcagcc caggcttatg ctaaagagaa
     1861 aggtttgacc cctccttcga cagaccatca ggattcagga aatactgagg caaaaggagc
     1921 agaagctatc tacaaccgcg tgaaagcagc taagaaggtg ccacttgatc gtatgcctta
     1981 caatcttcaa tatactgtag aagtcaaaaa cggtagttta atcatacctc attatgacca
     2041 ttaccataac atcaaatttg agtggtttga cgaaggcctt tatgaggcac ctaaggggta
     2101 tactcttgag gatcttttgg cgactgtcaa gtactatgtc gaacatccaa acgaacgtcc
     2161 gcattcagat aatggttttg gtaacgctag cgaccatgtt caaagaaaca aaaatggtca
     2221 agctgatacc aatcaaacgg aaaaaccaaa cgaggagaaa cctcagacag aaaaacctga
     2281 ggaagataag gaacatgatg aagtaagtga gccaactcac cctgaatctg atgaaaaaga
     2341 gaatcacgct ggtttaaatc cttcagcaga taatctttat aaaccaagca ctgatacgga
     2401 agagacagag gaagaagctg aagatactac agatgaggct gaaattcctc aagtagagca
     2461 ttctgt
//
LOCUS       DAVLYR010000181         2463 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLYR010000181 DAVLYR010000000
VERSION     DAVLYR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.181"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            105..1112
                     /locus_tag="VYO63_001863"
     CDS             105..1112
                     /locus_tag="VYO63_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1672728.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1115..1315
                     /locus_tag="VYO63_001864"
     CDS             1115..1315
                     /locus_tag="VYO63_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1672729.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1467..1649
                     /gene="rpmF"
                     /locus_tag="VYO63_001865"
     CDS             1467..1649
                     /gene="rpmF"
                     /locus_tag="VYO63_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1672730.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1665..1814
                     /gene="rpmG"
                     /locus_tag="VYO63_001866"
     CDS             1665..1814
                     /gene="rpmG"
                     /locus_tag="VYO63_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1672731.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2293..>2463)
                     /locus_tag="VYO63_001867"
     CDS             complement(2293..>2463)
                     /locus_tag="VYO63_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1672732.1"
                     /translation="VKDKGLWYYLNESGSMATGWFTVSGQWYYTYNSGDLLVNTTTPD
                     GYRVNANGEWVG"
BASE COUNT          817 a          469 c          450 g          727 t
ORIGIN      
        1 gaaattattg ggctgaaact ttttagtgat tattttcaac tttctattga cttttaaaaa
       61 caaagtgata tcatttagat atcaaataat aggaggtcat tcttatgact gaaaaactca
      121 tcaattcaaa accaaatggt gtattcgcat tgattctcat tgaattgaca atcgtacttg
      181 gtatctttat atttataatg ggtgttggtt cggaaaacat ttttggaatt attatcggac
      241 ctttactaat cgtaattgca ggtctagctc atgctggttt aaaagttgtc aaacctcagg
      301 aagctctggt tctgacactc tttggtaatt atacaggtac catcaaagaa cctggttttt
      361 actttgtcaa tcccttcagc gtagcagtca accctgcaaa ccacactcga cttggacaaa
      421 gtggtgatgt tagcacaaaa tctccttttt taggagctaa atcatcaaat gacaatgatg
      481 taaatcttga aattggcaag aaacagattt ccctcaaagt catgaccttg agcaattctc
      541 gtcaaaaaat caatgattgc ttaggaaacc ctgtagaaat cggtatcgcg gtaacttgga
      601 gagttgtcga taccgctaag gcagtcttca acgttgataa ctacaaagaa tatctttcat
      661 tgcaatgtga tagcgccctc cgtaatattg tccgcatcta tccttacgat gtgtctccta
      721 atgtggatac tacgggtgac gggcaagcag atgaaggtag tctccgtggc tctagcgaaa
      781 ttgttgctaa ccgtattcgt gaagaaatcc aaagtcgtgt tgaggatgct ggcttggaaa
      841 tccttgaagc acgtatcact tacctagctt atgctccaga aattgctgcc gttatgcttc
      901 aacgccaaca agcatctgcc attattgatg cacgtaagat gattgtagat ggtgctgtag
      961 gaatggttga aatggcacta gaacggctca atgaagggga attggtagaa cttgacgaag
     1021 aacgaaaagc tgccatggtt tcaaatctcc ttgtcgttct ttgtggcaat catgatgcac
     1081 aaccaattgt caacacagga agtctttact aaagatggct gaagctaaaa aaaagcagat
     1141 cccccttcga ctctcaacaa agttatacgc tgcactcgca tcatgggcag aagacgactt
     1201 tcgttcagtc aatgggcaaa tagaatatct cctcacagag tgtgtcaagc aacggaaaaa
     1261 agacggaaaa tacgtatcag aaaccattga cgaaccattt gagattgata tttaacacct
     1321 tctcctgtct gccgtgtcgg ataggagaat tttttcatct ttttttgtca agtaacttta
     1381 ctttacaaaa aaaatgtgtt atcctagtat ggttgatgaa aatcagtaga ttgaatcgaa
     1441 tataaatacc taaaggagaa atcaaaatgg cagtacctgc acgtcgcact tcaaaagcga
     1501 agaaaaacaa acgtcgtaca cactacaaag taacagctcc atctgtaaac tttgacgaaa
     1561 ctactggaga ttactcacgt tctcaccgtg tatcacttaa aggatactac aaaggacgta
     1621 aaatcgctaa agctgcatca gctgaataat agaagggaga tactatgcgc gtaaatatta
     1681 cacttgaaca caaagaatct ggtgaacgct tgtaccttac ttctaaaaac aaacgtaaca
     1741 ctccagaccg ccttcaattg aagaaatact caccaaaact tcgcaaacac gttgtgttta
     1801 cagaagtgaa gtaagcattc aatacgaatc aatacagaag aaaaacgctt gatcagcgtt
     1861 ttttcttttt gttaatttca ctatattgca agggaaatca ttcgaatgac tacattattg
     1921 aaataagaat acggattcgg acaattaaaa gcaagaggaa ctcaaatgta agttggggtt
     1981 ccttttttgt atatttttca aataacgcct ctacactatt tgtagcaaat tcaccaacta
     2041 cagttgtatc ttagttaaaa taagttagaa tatgtaagtg agtaccagat ataccaagac
     2101 atcgtcacca tctaaggtat attcaaaata caaaagttga ccaactagat ttctgaatat
     2161 ccttatatat ccattcttaa aattggttta aatagcgtag tcttttaaac tagttttgag
     2221 aatccaaaaa atcttcctac atatgtaaga agatttttta gttcagaatg attagattta
     2281 gctaatggat acctatccta cccactcacc gttagcattg actcgatagc catcgggtgt
     2341 agtcgtgttt actaataaat ctcctgaatt ataggtatag taccattgac cagaaactgt
     2401 aaaccaacca gtagccattg aacctgattc atttaagtaa taccataagc ctttgtcttt
     2461 aac
//
LOCUS       DAVLYR010000182         2447 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLYR010000182 DAVLYR010000000
VERSION     DAVLYR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.182"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            129..1622
                     /locus_tag="VYO63_001868"
     CDS             129..1622
                     /locus_tag="VYO63_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1672733.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2447
                     /locus_tag="VYO63_001869"
     CDS             1635..>2447
                     /locus_tag="VYO63_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1672734.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELI"
BASE COUNT          872 a          355 c          482 g          738 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcta
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggaa
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctaatagca tgggatggtg ctaatgcagg aacagttggc tatgggttat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagatcatt
     1981 caacaggtgc aactattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
     2101 ttactaaaag aaaatttcag ttagatgaac taaacttgct cgtcaaatcc cgatttaacg
     2161 agatgtttgg ggaaaataaa atatttgaaa gcattgataa cttatttgat attatagatg
     2221 gtgatagggg caaaaattat cctaaatcag atgagttgtt tagtgaggag tactgtttat
     2281 ttttaaatac aaagaatgtt actaaaaacg gattttcatt cgatacaaag caatttatca
     2341 ctaaaacaaa ggataaatta cttcgaaaag gcaaacttga gcgttatgat atagtcttga
     2401 caacaagagg tactgttgga aatgtagcgt actacgatga attaata
//
LOCUS       DAVLYR010000183         2442 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLYR010000183 DAVLYR010000000
VERSION     DAVLYR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.183"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            19..936
                     /gene="adcAII"
                     /locus_tag="VYO63_001870"
     CDS             19..936
                     /gene="adcAII"
                     /locus_tag="VYO63_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1672735.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            944..>2442
                     /locus_tag="VYO63_001871"
     CDS             944..>2442
                     /locus_tag="VYO63_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1672736.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT          815 a          449 c          516 g          662 t
ORIGIN      
        1 atggttaagg agttgttcat gaagaaacaa aatttatttt tagtcctgtt aagtgtcttt
       61 cttttgtgtt tgggggcttg tggtcaaaag gaaagtcaga caggaaaggg gatgaaaatt
      121 gtgaccagtt tttatcctat ctacgctatg gttaaggaag tatctggtga cttgaatgat
      181 gttcggatga ttcagtcaag tagtgggatt cactcctttg aaccttcggc aaatgatatc
      241 gcagccatct atgatgcaga tgtctttgtt taccattctc atacactcga atcttgggca
      301 ggaagtctgg atccaaatct aaaaaaatcc aaagtgaagg tcttagaggc ttctgaggga
      361 atgaccttgg aacgtgtccc tggactagag gatgtggaag caggggatgg agttgatgaa
      421 aaaacgctct atgaccctca cacatggcta gatcctgaaa aagctggaga agaagcccaa
      481 attatcgctg ataaactttc agaggtggat agtgagcata aagagactta tcaaaaaaat
      541 gcgcaagcct ttatcaaaaa agctcaggaa ttgactaaga aattccaacc aaaatttgaa
      601 aaagcgactc agaaaacatt tgtaacacaa catacagcct tttcttatct agcgaagaga
      661 tttgggctta atcaacttgg tattgcaggt atctctcctg aacaagaacc aagtccacga
      721 caactaacag aaattcagga atttgttaag acctataagg ttaaaacgat ttttacagaa
      781 agtaacgctt cttcaaaagt agctgaaact cttgtcaaat caacaggtgt gggtcttaaa
      841 actctgaatc ctttagagtc agacccacaa aatgacaaga cctatttaga aaatcttgaa
      901 gaaaatatga gtattctagc agaagaatta aagtgaggaa agaatgaaaa tcaataaaaa
      961 atatctagca ggttcagtgg cagtccttgc cctaagtgtt tgttcctatg aacttggtcg
     1021 tcaccaagct ggtcaggtta agaaagagtc taatcgagtt tcttatatag atggtgatca
     1081 ggctggtcaa aaggcagaaa atttgacacc agatgaagtc agtaagagag aggggatcaa
     1141 cgccgaacaa attgttatca agattacgga tcaaggttat gtgacctctc atggagacca
     1201 ttatcattac tataatggca aggttcctta tgatgccatc atcagtgaag aacttctcat
     1261 gaaagatccg aattatcagt tgaaggattc agacattgtc aatgaaatca agggtggcta
     1321 tgtgattaag gtagacggaa aatactatgt ttaccttaaa gatgcagccc atgcggacaa
     1381 tattcggaca aaagaagaga ttaaacgtca gaagcaggaa cgcagtcata atcacaactc
     1441 aagagcagat aatgctgttg ctgcagccag agcccaagga cgttatacaa cggatgatgg
     1501 gtatatcttc aatgcatctg atatcattga ggacacgggt gatgcttata ttgtccctca
     1561 cggcaatcac ttccactata ttcctaagag tgatttgtct gccagtgaat tagctgctgc
     1621 ccaagcctat tggaatggga agcagggatc tcgtccttct tcaagttcta gtcataatgc
     1681 aaatccagct caaccaagat tgtcagagaa ccacaatctg actgtcactc caacttatca
     1741 tcaaaatcaa ggggaaaaca tttcaagcct tttacgtgaa ttgtatgcta aacccttatc
     1801 agaacgccat gtagaatctg atggcctagt atttgatccg gcacaaataa catatcgtac
     1861 agtcaatggt gttgctgtac cgcacggaga ccattatcac tttattcctt attcacaact
     1921 gtcacctttg gaagaaaaat tggctcgtat gattgctgtt aagggacaaa atggtgcagt
     1981 cttgccagga atgcattatt tgaaaccagc accaaaaccg caagttcaac catcaactga
     2041 gaaaaaacaa actgattttg cagtagaaca ggttgttaga aaagttggag aaggctatgt
     2101 tgtggaaatt gctggtgtca gtcactatgt ttttgctaaa gacttggcta aggataagat
     2161 tgatgctatt gaaaatctct tgtctaagaa aactcaagag acacatgctc tagttgctaa
     2221 aaaagaaaat gtcgctcctc gtgaccaaga attttatgat aaagcttaca atctgttaac
     2281 ccaggctcat aaagtcttgt ctgaaaataa aggtcgtact tctgatttcc aagccttaga
     2341 caaattagca gaacgcttga ataatgaatc ttctaataaa gtaaaattgg tagatgattt
     2401 attggcattc ctagcaccaa ttacccaccc agaacgcctt gg
//
LOCUS       DAVLYR010000184         2419 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLYR010000184 DAVLYR010000000
VERSION     DAVLYR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.184"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            495..2333
                     /locus_tag="VYO63_001872"
     CDS             495..2333
                     /locus_tag="VYO63_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1672737.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASSPTVVRAEEAPVASQSKAEK
                     DYDTAKRDAENAKKALEEAKRAQEKYADYQRRIEEKAAKETQASLEQQEANKDYQLKL
                     KKYLDGRNLSNSSVLKKEMEEAEKKDKEKQAEFNKIRREIVVPNPQELEMARRKSEVA
                     KAKESGLVKRVEEAEKKVTEARQKLDAERAKEVVLQAKIAELENEVHKLEQKLKEIDE
                     SDSEDYVKEGFRAPLQSELDAKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDG
                     EQAGQYLAAAEEDLIAKKAELEQTEADLKKAVNEPEKPAPAPAPAPETPAPEAPAEQP
                     KPAPETPAPAPKPEKPAEQPKPEKPADQQAEEDYARRSEEEYNRLTQQQPAPAPKPEQ
                     PAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANG
                     SMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNSNGAMATGWLQYNGSWYYLN
                     ANGDMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYY
                     LEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYEVNANGEWV"
BASE COUNT          913 a          409 c          535 g          562 t
ORIGIN      
        1 ataagttgtt gcatcgtagc tagggattta tctaaaaata ctatgaaatt ggacgtcata
       61 ttttttgagg ataaaaataa aagatccgca gttttaggtt tacgaagaga caaaagcgga
      121 gtatttaaac cagttactct acattttaca agcgctaaga aatatgctaa agttcgtaaa
      181 acagatgtga aagaaatgaa atggctataa agattgtccg caggcttaag cttgcgtttt
      241 ttgttgttta acgaaattaa aaaacaagct ctttcatcga aaatgttttt ataggaatga
      301 aggaagatga tgctatgttt aaaaagagaa tcgaaaaggg caaaagtagt atctttatct
      361 ttttggagta gaagttatga tatagaaatt tgtaataaaa atgtaatata aaacatttga
      421 caaatattta cggggggagt atacttaata taagtatagt ctaaaaatga ctatcagaaa
      481 agaggtaaat ttagatgaat aagaaaaaaa tgattttaac aagtctagcc agcgtcgcta
      541 tcttaggggc tggttttgtt gcgtcttcgc ctactgttgt aagagcagaa gaagcccccg
      601 tagctagtca gtctaaagct gagaaagact atgatacagc gaagagagac gctgagaatg
      661 cgaaaaaagc tttagaagaa gcaaaacgtg cgcaggaaaa atatgccgat tatcagagga
      721 gaattgagga gaaagcggca aaagaaacac aagcttctct agagcaacaa gaagcaaata
      781 aggactatca attaaagttg aagaaatatc ttgacggaag gaacctctca aatagttctg
      841 tgttaaagaa agaaatggag gaagctgaga aaaaagataa ggaaaaacaa gcagaattta
      901 ataagattag acgagagata gtggttccta acccgcaaga gttagaaatg gctagacgaa
      961 aatcagaagt ggctaaagca aaagaatcag gacttgttaa aagagtagaa gaagctgaga
     1021 aaaaagttac tgaagccaga caaaaattgg atgctgaacg tgctaaagaa gttgttcttc
     1081 aagccaaaat cgctgagttg gaaaatgaag ttcataaact agaacaaaag ctcaaagaga
     1141 ttgatgagtc tgattcagaa gattatgtta aagaaggttt ccgtgctcct cttcaatctg
     1201 aattggatgc taagcaagct aaactatcaa aacttgaaga gttgagtgat aagattgatg
     1261 agttagacgc tgaaattgca aaacttgaaa aagatgtaga agatttcaaa aactcagacg
     1321 gtgagcaagc tggacaatac cttgctgcag ctgaagaaga cttaattgct aaaaaagctg
     1381 aattagaaca aactgaagct gaccttaaga aagcagttaa tgagccagaa aaaccagctc
     1441 cagctccagc tccagctcca gaaactccag ccccagaagc accagctgaa caaccaaaac
     1501 cagctccaga aactccagct ccagcaccaa aaccagagaa gccagctgaa caaccaaaac
     1561 cagaaaaacc agctgatcaa caagctgaag aagactatgc tcgtagatca gaagaagaat
     1621 ataaccgctt gactcaacag caaccagctc cagctccaaa accagagcaa ccagctaagc
     1681 cggagaaacc agctgaagag cctactcaac cagaaaaacc agccactcca aaaacaggct
     1741 ggaaacaaga aaacggtatg tggtatttct acaatactga tggttcaatg gcaacaggtt
     1801 ggctccaaaa caacggttca tggtactacc tcaacgctaa cggttcaatg gcaacaggtt
     1861 gggtgaaaga tggagatacc tggtactatc ttgaagcatc aggtgctatg aaagcaagcc
     1921 aatggttcaa agtatcagat aaatggtact atgtcaacag caatggcgct atggcgacag
     1981 gctggctcca atacaatggc tcatggtact acctcaacgc taatggtgat atggcgacag
     2041 gatgggctaa agtcaacggt tcatggtact acctaaacgc taacggtgct atggctacag
     2101 gttgggctaa agtcaacggt tcatggtatt acctcaacgc taacggttca atggcaacag
     2161 gttgggtgaa agatggagat acctggtact atcttgaagc atcaggtgct atgaaagcaa
     2221 gccaatggtt caaagtatca gataaatggt actatgtcaa tggcttaggt gcccttgcag
     2281 tcaacacaac tgtagatggc tatgaagtca atgccaatgg tgaatgggtt taagccgatt
     2341 aaattaaatc atgttaagaa catttgacat tttaattttg aaacaaagat aaggttcgat
     2401 tgaataaatt tatgttcgt
//
LOCUS       DAVLYR010000185         2381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLYR010000185 DAVLYR010000000
VERSION     DAVLYR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.185"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..863
                     /locus_tag="VYO63_001873"
     CDS             <1..863
                     /locus_tag="VYO63_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1672738.1"
                     /translation="SLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDID
                     LKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILI
                     DVKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTD
                     FIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKI
                     QESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIE
                     KTKMAEEFIY"
     gene            874..1458
                     /locus_tag="VYO63_001874"
     CDS             874..1458
                     /locus_tag="VYO63_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1672739.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1458..2153
                     /locus_tag="VYO63_001875"
     CDS             1458..2153
                     /locus_tag="VYO63_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1672740.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKIAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2141..>2381
                     /locus_tag="VYO63_001876"
     CDS             2141..>2381
                     /locus_tag="VYO63_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1672741.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISM"
BASE COUNT          853 a          314 c          462 g          752 t
ORIGIN      
        1 actctttaga tgcatctaaa gagggcttga tggaaatatt ttatttatcg cccaaaatag
       61 aaagattaaa agaaattcaa aatcaagatt tacaagaagg cgatgactat agcttaaaaa
      121 aatttgatat tgatctaaaa gatgttgagt ttgcctacaa taaagacgca aaagttttaa
      181 atggtgtaag ttttaaagct aagcagggag aggtcactgc tttggtaggt gcaagtggct
      241 gcggtaaaac aactatcttg aaacttatat caagacttta tgattatgac aagggacaaa
      301 tcttaatcga tgtcaaagat ataaaagaaa tatcaacaga atctcttttt gataaggtct
      361 ctatagtttt ccaagatgtg gttctcttta atcaaagcgt tatggaaaat attagaatcg
      421 gaaagcaaga tgcaagtgac gaagaggtta aaagagcagc aaaacttgca aattgcacag
      481 attttataga aaaaatggat aaaggtttcg atacagttat tggtgagaac ggagctgagc
      541 tatcaggagg agaaagacaa agattatcaa tagccagagc cttcttaaaa gatgcgccga
      601 tattgatctt agatgagata acagcaagcc ttgatgttaa caacgagaaa aagattcaag
      661 agtctttaaa taatttagtt aaagataaaa ctgttgtaat catttcacat agaatgaaat
      721 ccatagaaaa tgcagacaag atagtagttc ttcaaaacgg aagagtagaa agcgaaggta
      781 agcatgaaga gcttttacaa aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa
      841 tggcagaaga atttatttat taggaggact acaatggata ataaaaaatt aaaagtaaaa
      901 gatttagtaa gtatcggtgt ttttggcgta atttattttg ccttcatgtt tggagttggt
      961 atgatgggct tgattccaat attgttctta atatacccga cagtattagc catagttgca
     1021 ggaactgttg ttatgttatt tatggctaag gttcaaaagc catgggcact atttatattt
     1081 ggtatgatat caccacttgt gatgtttgca gctggtcata cctacgtagt tgtggtttta
     1141 tcacttatag taatgataat agcagaatta attagaaaga ttggtaatta taattcattt
     1201 aaatacaata tgctttctta tgcaatcttc agcacatgga tatgtagctc tttaatgcaa
     1261 atgcttttag caaaagaaaa atatatggag tggtctttga tgacgatggg aaaagattat
     1321 gttgatgtat tagaaaagtt aataacttat cctcacatgg ctttagtagc cttaggtgct
     1381 ttcttaggag gaattcttgg agcatatata ggcaaggctc tattgaaaaa acactttgaa
     1441 aaagcaggca tcgtataatg cctacttttt cttcaaatgc tccctttaat cccaatccga
     1501 tctctaagat attagtcgta tttatcacag gcttaactgt tatgcatagc ataaacatcc
     1561 gttttgagct ggcaattgtt tgtcttattg gtattttgct ttatttgaat ggatacaaaa
     1621 aaacactttt caaatggatt atattgtgtg ggatacttta ctccttgcct aattttatgg
     1681 tgttatctga attaaaccct atagttaaga tgtttttgag tatacctatt gttattagaa
     1741 tgtttatttt accatttatg gcagcaagct ttatgataaa gacctcggat gtaggcgcaa
     1801 taatttcatc gatggataag cttaagattt caaagaatgt atccatacct attgcggtta
     1861 tgtttagatt cttcccatct tttaaggagg agaagaaaaa catcaaaata gctatgagag
     1921 taagagggat aaattttaaa aacccagtca aatatcttga atatgtttct gtgccactac
     1981 tcattatatc atctaatata tcagatgaca ttgcaaaagc ggcagaaaca aaggcaatag
     2041 aaaatccaat tgccaagacc agatacattc gcgtaaagat acagctaatt gattttgttt
     2101 atgttttagc ggttgctgga cttattgtgg gaggcttaat atggttgaaa taaaaaattt
     2161 aagtcttgat tatggtgaag agcatatatt agatgatata tcactatcca tagccgaggg
     2221 agagtgcgtg ctatttacag gaaaaagtgg aagtggtaag tcatctttaa taaattcaat
     2281 caatggacta gctgtaaggt atgataacgc aaagacaaag ggcgaaataa ttattgatgg
     2341 taagaatata aaaaatttgg aactttatca aatctcaatg c
//
LOCUS       DAVLYR010000186         2372 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLYR010000186 DAVLYR010000000
VERSION     DAVLYR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.186"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(83..1366)
                     /locus_tag="VYO63_001877"
     CDS             complement(83..1366)
                     /locus_tag="VYO63_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1672742.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMLATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTSGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(1620..2333)
                     /gene="rsmG"
                     /locus_tag="VYO63_001878"
     CDS             complement(1620..2333)
                     /gene="rsmG"
                     /locus_tag="VYO63_001878"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1672743.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
BASE COUNT          743 a          466 c          502 g          661 t
ORIGIN      
        1 taataatttt gttaagacac ttttattata gtatacgaaa aatccactca gctgtctgag
       61 tggattaggt gtatttagac tcttagtctt tattttcgta tggcaagatc aagttcaaga
      121 tgattcctgt catggctgaa agggcagtac ctgaaagtgt aactggacca agtttaagga
      181 tagctcctcc aagtccaaga accaacatag cacttgcgat gatgaggttt cgcatttgag
      241 cgaaatcaac acgttcttta atcaagactt tcaaaccatt gctggcgata accccataga
      301 gaaggattga cataccacca agtacagcgt ttggaatagt tgaaatcaag gcagtgaatt
      361 taccaaggaa gctgagggca atcgcgatga aggcagcgtt acggataact gagacagaag
      421 cgatacgagt cataccgata acccctgtat tttctccgta agttgtattg gctggtccac
      481 caaggaaggc agaaacagaa gttgcgatac cgtcaccaat aagagtacgg tgaagacctg
      541 gttcttttaa gaattgacgg ccacagattt gacccaaaac agtatggtct ccgatatgtt
      601 cagaaattgt tacgatagcg attggcaaga tagcgatggc ttctggacca aagtaaagat
      661 tgtactcttt aaaggcacca cttgtgctaa atggcaagta gaaaccagga atttcaaacc
      721 agttagcttc taggacaggc gtaaagtcaa ccaaaccaag agttagtgcg aagaggtaac
      781 caccgataat ggcaaagagg aatggaatga ttcgtaggaa gccttttcct tttgtattga
      841 taaaggcagc aattaggaaa gtaacaacgg ctaccagagc atttttccaa tttccgtctg
      901 ctacaagacc tgcattggta acagctgaac ctgcaagtcc aagaccgata acgatgatca
      961 taggaccgat aatgattggt ggcaagagtt tatcaatcca ttttgttcct acaaatcgga
     1021 tgctggtagc aacaaggaca tagaccaaac cagtcaagat aacccctgtt tgagcagcag
     1081 atacatcccc ccccatttct ttcatagcca gtgacatagc tgtgataaag gcgaatgaag
     1141 aacctagata aactggaact ttaaaaccag tagcaagcat gtagatgagt gttccaacac
     1201 ctgaagcaaa aagggcaaca gatacaggca ttcccaaaat caatggtacc aagatggtcg
     1261 caccaaacat ggcgaaaacg tgttggaaac taaggagaat tccttttcca gccgaaggac
     1321 gttggtcaac gtctagtaac aaatcaacag ttgattcttg tttcatataa acctcacttt
     1381 tgggcatcaa aaaagagccc attattttac agcaataggc cctcagagta agacttcttt
     1441 aaaatttaga ttttaaagag ttttaaatat ttctgcgtct tcgtcacctc acgggatgac
     1501 attaaaaacc tttgcttagg atagtatagc agaaaaaaat gattttgtaa atcatttttt
     1561 cccgagccta gaaataaaag agcgaggctg attttgtaaa catttgttta ctaaaaaatt
     1621 taaagtgggc gtttatttgg cataccagcc ttacgtggat atttatttgg tgtttctttt
     1681 ttcttttcta ccactgtgat atagcgcgga tctctattcg gtagggcgta gctgagattg
     1741 tcttcgacct tactaaaaag gagattgagg gcattcttag cttctaataa ttcctcaggc
     1801 gcattgctgg ccttgagtgc caatagtttt ccaccaacct taaggtaggg aatggtcaat
     1861 tcagatagga cctgcatacg ggcaaccgca cgagctgtta caaaatcata ttgagcacgg
     1921 aagttcttgt cttgggcaaa atcttcggca cgtccgtggt agaaatgaac tccgttcaaa
     1981 tccagttctt gagccaagag ttgtaggaag ttgatgcgct tattgagtga atcaataatg
     2041 gtcacatcta actccggata gagaattttc attggtagac taggaaatcc tgccccagcc
     2101 ccgatatcaa gaagtttgat agtttcattg ggaatcaaac cttgaagaat gggtgcaatc
     2161 gaatcgtaaa aatgtttgag ataaacttct tccttgtccg taatcgccgt caaattaatc
     2221 ttctcattcc actcgaccaa gagctcaaaa taacgttcaa attgttcttt ttgctggtcc
     2281 gaaagtggaa gattctgctc ggcaagcaag ttgtaaaatg tttttggttt catagttatt
     2341 tccttcttta gtatcatctt attttaccac aa
//
LOCUS       DAVLYR010000187         2346 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLYR010000187 DAVLYR010000000
VERSION     DAVLYR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.187"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..299
                     /locus_tag="VYO63_001879"
     CDS             <1..299
                     /locus_tag="VYO63_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1672744.1"
                     /translation="IAKQRSFIDYWAKYVSYLPFPTPEFFLANLCNSVDREGYDFSKD
                     GNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKIQPESSMFQCIKEKLEALF"
     gene            679..1482
                     /locus_tag="VYO63_001880"
     CDS             679..1482
                     /locus_tag="VYO63_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1672745.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1598..2346)
                     /locus_tag="VYO63_001881"
                     /pseudo
     CDS             complement(1598..2346)
                     /locus_tag="VYO63_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          717 a          410 c          434 g          785 t
ORIGIN      
        1 taattgcgaa acaaagaagc tttatagatt attgggctaa atacgtttct tatcttcctt
       61 tcccaacccc agagtttttt ttagctaatt tgtgtaacag cgtagatagg gaaggttatg
      121 atttttctaa agatggcaac ggaaaagaat attttagaaa aaaaactcaa gtagcgttag
      181 gaatagagaa tataacttct gaagatattt tccaggagca aagaagagct gtttcaaaaa
      241 tacagccaga atcatcaatg tttcagtgta ttaaagaaaa attagaggca ttgttctaat
      301 tccttagatt aggtactaca taacaaattt tttaaattca aacccgcacc aaaaacaaca
      361 ttgcaacttt cttctcaggt aatgtgttgg gatttgaaca gaccaacgag ttcatagagg
      421 tgtatgccaa tgagtttgat aagtatgcgc gtcagactta ttagttcatt tatccagata
      481 tatctcgata atcatattat ttagtcaaag aacatcccat atgagggcat gaaatgctaa
      541 gtttagcttg ctcactatta agatgaagta tatatatttc atagaactta ttgtgaagga
      601 tttttataat tttcgccaat tccataccca tttcaccatt taccgatgac acagaaaaac
      661 aattgaaagg atatttctat gcaaccaact tacaacattg acaatccaaa cttgtcttat
      721 gaagcgaagc gtgatttatg gcggataggt tttggtctgc agaaagttga caatctagtg
      781 ccatcagcgt atatggaatc tttggctgag aaacagtccc ggggagaact gacttatgag
      841 caggtttatg aggatgcaac ggcttatcac catactattg atgcaagtac ggaagaggca
      901 gacttggttt ctctacgtat tgtagaacta ttgtctcgaa gaggctttag ctttagtcct
      961 gcgaccttac ttgctattca taaggagttg tttcaagata tatttgaacc ctctattccg
     1021 gtggggcaat ttcgtcagac caatatcaca aagaatgaac ctgttttgaa tggtgaaagt
     1081 gttgtgtact ctgattactc catgattcaa atgaccttag attatgattt taatcaggaa
     1141 aaacaagttg catatgcgac actaactcag gcggatgtgg ttaaacaaat ccagcatttt
     1201 atttcaggaa tctggcagat tcatccattt cgcgaaggaa acactcggac agtaacggtc
     1261 tttttgattc agtatcttcg tgagtttggt tttgatattg ataatacacc atttcagcaa
     1321 cattccaaat attttcgtga tgccttggta ttagataatg caaagatttt acagcgacgt
     1381 cctgagtttt taacagcttt ttttgaaaat ctcttgctcg gtggtcaaaa tgatttgtct
     1441 tcagaaaaaa tgtatctaga tttagacctt gatttttcat aatccaaata ctgagtaaac
     1501 attgaatttt aggcaaaaat gaagtaaata ttctcacaag aaaacgtata tcatcaaagt
     1561 tttaggtgta ctgaccccaa acagttagac aattaattta tccgaaggat ttagttctgt
     1621 attgcacagg actaagtcct tttagtttta ccttaattcg tttattgttg tagtaatcaa
     1681 tatagtctac aatagcttgt tccaattgct taagcgactg aaacgacttc tcataaccgt
     1741 aaaacatttc cgatttcaga atcccaaaga aagattccat cataccgttg tcttggctgt
     1801 ttcccttgcg tgacatggat gcttgaactc ccttactctc taggaaccga tgataagaat
     1861 cgtgtcggta ttgccagcct tggtcactat ggagaatcgt attctcatag tgcttctctg
     1921 tgaatgcctg ttccaacatt gtttgtactt gttctaagtt gggtgaagtt gaaagattat
     1981 aggcgataat ttcgctatta aagccatcta aaactggtga taagtaaagc ttttgagtac
     2041 ttgctggaat ggcaaattct gtcacatctg tgtagcactg ttccattgtt ttagagcctt
     2101 caaattggcc ttgaatgaga ttctctgcct tcttgccaac gtctccttta tgagaagaat
     2161 attttcgttt ctgtcgcatt ttagcttgta aattgagtac ttcatcaagt gttgaactct
     2221 tttatgattt accagataac cacgatttct tagttctaaa taaatccgac gataaccata
     2281 atttcccttg tgttcgataa agatggattg aatttcagct ttaagctctt ggtccttatc
     2341 tgtttt
//
LOCUS       DAVLYR010000188         2330 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLYR010000188 DAVLYR010000000
VERSION     DAVLYR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.188"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..2312
                     /gene="mfd"
                     /locus_tag="VYO63_001882"
     CDS             <1..2312
                     /gene="mfd"
                     /locus_tag="VYO63_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1672746.1"
                     /translation="ILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNL
                     RHGFHFVDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGI
                     GQYLGIETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDG
                     HFKKAKQKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQL
                     RSIEEIKRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQ
                     HYTNFKERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFAD
                     LGLMIIDEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPP
                     TNRYPVQTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASI
                     GYVHGRMSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTL
                     YQLRGRVGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRG
                     AGNLLGKSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPD
                     TYISDQRHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDK
                     VFVQRVERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKELMELVFDVQN
                     KKDYEILEGLLIFGESLLEIKESKEENSI"
BASE COUNT          766 a          344 c          503 g          717 t
ORIGIN      
        1 ttattctgca gtctagcaat tcaatgggaa gtaaaacatt ggaggatatg ttagaggaat
       61 atcagattaa attggattct agagataaga caaatatctg taaagaatct gtaaacttaa
      121 tagagggtaa tctcagacat ggttttcatt ttgtagatga aaagatttta ttgataactg
      181 aacatgagat ttttcaaaag aaattaaagc gtcgttttcg aagacaacat gtttcaaatg
      241 cagagagatt aaaagattac aatgaacttg aaaaagggga ctatgttgtc catcatatcc
      301 atgggattgg tcaatatcta ggaattgaaa ccattgaaat caagggaatt catcgcgatt
      361 atgtcagtgt ccaataccaa aatggtgatc aaatttctat ccccgtggaa cagattcatc
      421 tactgtccaa atatatttca agtgatggta aagctccaaa actcaataaa ttaaatgacg
      481 gtcattttaa aaaggccaag caaaaggtta agaaccaggt agaggatata gctgatgatt
      541 taatcaaact ctactctgaa cgtagtcagt tgaagggttt tgctttctca gctgatgatg
      601 atgatcaaga tgcctttgat gatgctttcc cttatgttga aacggatgat caacttcgta
      661 gtattgagga aatcaaaagg gatatgcagg cttctcagcc aatggatcga cttttagttg
      721 gggatgttgg ttttggaaag actgaagttg ctatgcgtgc agcctttaaa gcagtcaatg
      781 atcacaaaca ggttgtcatt ctagttccga cgacggtttt agcgcaacag cactatacga
      841 attttaagga acgattccaa aattttgcag ttaatgttga tgtgttgagt cgctttagaa
      901 gtaaaaaaga gcagactgca acacttgaaa aattgaaaaa cggtcaagtc gatattttga
      961 ttggaacaca tcgtgttttg tcaaaagatg ttgtgtttgc tgatttgggc ttgatgatta
     1021 ttgatgagga acagcgattt ggtgtcaagc ataaggaaac tttgaaagaa ctgaagaaac
     1081 aagtggatgt cctaaccttg accgctacgc caatccctcg taccctccat atgtctatgc
     1141 tgggaatcag agatttgtct gttattgaaa ctccgccgac taatcgctat cctgttcaga
     1201 cctatgtttt ggaaaagaat gatagtgtca ttcgtgatgc tgtcttgcgt gaaatggagc
     1261 gtggaggtca agtttactat ctttacaaca aagttgacac aattgttcag aaggtttcag
     1321 aattacagga gttgattccg gaggcttcga ttggatatgt tcatggtcga atgagtgaag
     1381 tccagttgga aaatactcta ttagacttta ttgagggaca atacgatatc ttggtgacga
     1441 ctactattat tgagacaggg gtggacattc caaatgctaa tactttattt attgaaaatg
     1501 cggaccatat gggcttgtca accttatatc agttaagagg aagagtcggt cgtagtaatc
     1561 gtattgctta tgcttatctc atgtatcgtc cagaaaaatc aatcagtgaa gtctctgaaa
     1621 agagattaga agcgattaaa ggatttacag aattgggctc tggctttaag attgcaatgc
     1681 gagatctttc gattcgtgga gcaggaaatc ttttaggaaa atcccagtct ggtttcattg
     1741 attctgttgg ttttgaattg tattcgcagt tattagagga agctattgct aaacgaaacg
     1801 gtaatgctaa cgctaacaca agaaccaaag ggaatgctga gttgattttg caaattgatg
     1861 cctatcttcc tgatacttat atttctgatc aacgacataa gattgaaatt tacaagaaaa
     1921 ttcgtcaaat tgacaaccgt gtcaattatg aagagttaca agaggagttg atagaccgtt
     1981 ttggagaata cccagatgta gtagcctatc ttttagagat tggtttggtc aaatcatact
     2041 tggacaaggt ctttgttcaa cgtgtggaaa gaaaagataa taaaattaca attcaatttg
     2101 aaaaagtcac tcaacgactg tttttagctc aagattattt taaagcttta tccgtaacga
     2161 acttaaaagc aggcatcgct gagaataagg aattaatgga gcttgtattt gatgttcaaa
     2221 ataagaaaga ttatgaaatt ttagaaggtc tgctgatttt tggagaaagt ttattagaga
     2281 taaaagagtc taaggaagaa aattccattt gatatttttc ttctataaaa
//
LOCUS       DAVLYR010000189         2327 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLYR010000189 DAVLYR010000000
VERSION     DAVLYR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.189"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            241..1512
                     /locus_tag="VYO63_001883"
     CDS             241..1512
                     /locus_tag="VYO63_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1672747.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(1532..>2327)
                     /locus_tag="VYO63_001884"
     CDS             complement(1532..>2327)
                     /locus_tag="VYO63_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1672748.1"
                     /translation="FVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVVATSPDYA
                     PFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGKADLAVAG
                     ISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTVPESMVK
                     EQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATVSLKMKDG
                     DANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
BASE COUNT          718 a          540 c          417 g          652 t
ORIGIN      
        1 attagcataa aaacttttta aaacaaatca accccagata aaaatacaca tgttgtttta
       61 aagacaaaaa agggtacgaa aaactgtcac atctgcattt gattgctctt tgatttcaaa
      121 aaagtattaa aaatgggagt ataccacatt tgcccctaga tgtgaacgag agaaacgctc
      181 tagacattgc caagaaggaa aaaaaagggt acaatgtaac aaaatcaagg gaggtctgga
      241 atgaagaaac aaagcaagta caaagaggtc gtttcctatc tgaaaaatgg tatcgagtct
      301 ggacgatttc cgacgggtag tcgcctgcct tctatccgtc aactgagcct tgactttcac
      361 tgcagcaagg acaccattca acgagccctg ctggaattac ggcacgaaca atatctctat
      421 gccaagcctc agagtggcta ctatgtatta gaacaagggc aacatcaaga cctagaaatc
      481 gaggttaccg acgaacatgc cagtgcctat gacgatttcc gactctgtgt caatgaaacc
      541 ttgattggcc gagaaaacta cctcttcaac tactatgaca atcaagaagg attagaagac
      601 ctaagacagt ccattcacaa actcctcttt gagcaagctc tctactgcaa ggctaaccaa
      661 ctagtactga cttctggaac ccaacaagcc ttgtttatcc tctctcaaat atcctttcct
      721 agacaagcca aggaaatctt ggtggaacag ccaacctacc atcggatgaa tcgcctcttg
      781 attgcacagg ggctggacta tcaaacgatt gaacgaggca ttgatgggat tgacttggag
      841 gagctggaag gccacttcaa aacaggaaaa attaaatttt tctacaccat tcctagattt
      901 cactatcctc tgggacattc ctattctgag caggacaaac gatctattct taacttagct
      961 gccaagtatg atgtctatat cgtagaggac gattatctgg gtgatttgga ctccaagaag
     1021 ggccaaacct tccactatct tgatacagag gagcgtgtca tttatatcaa gtccttctcg
     1081 accagccttt ttcctgccct tcgtattaca gcactcattc ttccaaatgc tatcaaagaa
     1141 gcatttgtgg cctacaaaaa tatcctagac tacgacagca acctcattat gcaaaaggcc
     1201 ctgtcactct atattgacag tcaattgttt gaaaaaaatc gtttggctcg cttgaccaat
     1261 catgaatctt accaaaaaca aatcgaggaa aggataacta aaacaccttg tccccttcct
     1321 cattatcccc tacacgatgg tttattgcta gacctgagac agtatcctaa aatcgccagt
     1381 ctcaaacaca gtcaactggg cttggacttc tttgaagagg cctatttaag cacctgtcct
     1441 tatcaatttg ccaaggtgtc cctagacaat ctggaaaatg ttttaaacta tttaaaagca
     1501 gaattggaat gacatccaac tctgcttttt cttattcttc aacttcagtt aaacttgctg
     1561 ctttttcaag atagctttgg taagttcctt catccttaag tttttggatg accttgtcta
     1621 ccacttcttt caaatcatca ctattttttc taagagcaac ggcattggcg tcgccgtcct
     1681 tcatcttcaa gctgacagtt gcgacagcta agccagcgtt tttagcagca taactaagtg
     1741 caacaggctc atccatatga acagcatcta cttttccagc ctgcaattca ttgactgctt
     1801 cacccatatt agttagggaa gttaattgag cttttggcaa ttgttccttg accattgatt
     1861 ctggaacagt ccctttttgg gctgcaatat tagcactttc taggctagtt aaatccttgt
     1921 atttttccac atcagcctta cgaaccaaga aactaatctt gttttcatag tatgggattg
     1981 aaaaatcaaa gacttctttt ctctcgtcag tagcactaat tcctgcaact gctaggtcag
     2041 ccttaccagt ttgaagactg gtcaaaacat tatcaaaact catgcttgag atttccaact
     2101 taaccccaag ttcatcagcg atagcctgag ccatgtcgat gtctgcaccg actacctggt
     2161 tctttccatc aaccaatgat tgaaattcaa agggtgcata gtcaggactg gttgccacaa
     2221 ctaatttccc cttttgctta atggcctcaa cagctgactg agaaccatta gaacctgact
     2281 ggcaagctac taagaagaaa cttgcaagaa aattacataa aacaaat
//
LOCUS       DAVLYR010000190         2303 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLYR010000190 DAVLYR010000000
VERSION     DAVLYR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.190"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            243..506
                     /locus_tag="VYO63_001885"
     CDS             243..506
                     /locus_tag="VYO63_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1672749.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            512..2245
                     /gene="ptsP"
                     /locus_tag="VYO63_001886"
     CDS             512..2245
                     /gene="ptsP"
                     /locus_tag="VYO63_001886"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1672750.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
BASE COUNT          739 a          448 c          506 g          610 t
ORIGIN      
        1 aaacacaata tatagtgttt gtattgtgaa atagcaatga ttatcctata aattgttttt
       61 gaagaaacta tatcctgtgt tttgttatct aggatagaag attttcagca agttatctga
      121 aaggaaattg aaggaaaatc cagaaaatgc ttgaaaaaaa tcctagaaga tggtataata
      181 ctaaattgta agggttatca catataactc aaaaaaagaa agaacaaaag gagagtcaaa
      241 ctatggcttc taaagatttc cacgtagtgg cagaaacagg tattcacgca cgtccagcaa
      301 cattgttggt acaaactgct agcaaatttg cttcagatat cactcttgag tacaaaggta
      361 aatcagttaa ccttaaatca attatgggtg ttatgagtct tggtgttggt caaggtgctg
      421 acgtaactat ctcagctgaa ggtgcagatg cagatgacgc tatcgctgca atctcagaaa
      481 caatggaaaa agaaggattg gcataaggga aatgacagaa atgcttaaag gaatcgcagc
      541 atctgacggt gttgcagttg caaaagcata tctactcgtt cagccggatt tgtcatttga
      601 gactattaca gtcgaagata caaacgcaga agaagctcgc cttgatgccg ctctacaggc
      661 atcacaagac gagctttctg ttattcgcga gaaagcagta ggtacgctcg gtgaagaagc
      721 agctcaagtt tttgatgctc acttaatggt tcttgctgac ccagaaatga tcagccaaat
      781 caaggaaact atccgtgcga agaaagtgaa tgcagaagca ggtctgaaag aagttacaga
      841 tatgtttatc actatctttg aaggcatgga agacaaccca tacatgcaag aacgcgcagc
      901 ggatatccgc gacgtgacaa aacgtgtatt ggcaaacctt cttggtaaaa aattgccaaa
      961 cccagcttct atcaatgaag aagtgattgt gattgcgcat gacttgactc cttcagatac
     1021 agctcaattg gacaaaaact ttgtaaaagc ttttgtaacc aacattggtg gacgtacaag
     1081 ccactcagct atcatggcac gtacacttga aattgctgct gtattaggta caaacaacat
     1141 cactgaaatc gttaaagacg gtgacatcct tgctgttaac gggatcactg gagaagtgat
     1201 tatcaacccg acagatgaac aagcggcaga atttaaagca gctggtgaag cctatgcgaa
     1261 acaaaaagct gaatgggcac ttttgaaaga tgctcaaaca gtgactgctg acggtaaaca
     1321 cttcgagttg gctgccaata ttggtactcc aaaagacgtt gaaggtgtta acaacaacgg
     1381 tgcagaagct gttggacttt accgtacaga gttcttgtac atggattctc aagacttccc
     1441 aactgaagat gagcagtatg aagcatacaa ggctgttctt gaaggaatga acggtaaacc
     1501 tgttgtcgtt cgtacaatgg atatcggtgg agataaggaa cttccttact tcgatatgcc
     1561 tcacgaaatg aacccattcc ttggattccg tgctcttcgt atctctatct ctgagactgg
     1621 agatgctatg ttccgcacac aaatccgtgc tcttcttcgt gcgtctgttc acggtcaatt
     1681 gcgtatcatg ttcccaatgg ttgcgctctt gaaagaattc cgtgcagcga aagcagtctt
     1741 tgatgaagaa aaagcaaacc ttcttgctga aggtgttgca gttgcggata acatccaagt
     1801 tggtatcatg atcgagattc ctgcagcggc tatgcttgca gaccaatttg ctaaagaagt
     1861 tgacttcttc tcaattggta caaacgactt gatccaatat acaatggcag cagaccgtat
     1921 gaacgaacaa gtttcatacc tttaccaacc atacaaccca tcaatcctac gcttgattaa
     1981 caatgtgatc aaagcagctc acgctgaagg taaatgggct ggtatgtgtg gtgagatggc
     2041 tggtgaccaa caagctgttc cacttcttgt cggaatgggc ttggatgagt tctctatgtc
     2101 agcaacatct gtacttcgta cacgcagctt gatgaagaaa ctcgacacag ctaagatgga
     2161 agagtacgca aaccgtgccc ttacagaatg ctcaacaatg gaagaagttc ttgaacttca
     2221 aaaagaatac gttaattttg attaatcgaa aagtccctgc aactcagtta cagggacttt
     2281 ttttgatatt ttaaaaagaa ttt
//
LOCUS       DAVLYR010000191         2280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLYR010000191 DAVLYR010000000
VERSION     DAVLYR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.191"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..240
                     /locus_tag="VYO63_001887"
     CDS             <1..240
                     /locus_tag="VYO63_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672751.1"
                     /translation="KQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLI
                     KRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(370..1185)
                     /locus_tag="VYO63_001888"
     CDS             complement(370..1185)
                     /locus_tag="VYO63_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1672752.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(1182..2087)
                     /locus_tag="VYO63_001889"
     CDS             complement(1182..2087)
                     /locus_tag="VYO63_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1672753.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
BASE COUNT          745 a          437 c          470 g          628 t
ORIGIN      
        1 aagcaggttc atcctctttt tcagactgtc tttaaaacct ttctaaagga caaagagaaa
       61 atcgtcaacg cccttcaact acactattct aacgccaaat tggaagcgac caataatctc
      121 atcaaactta tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt caaaaaacgg
      181 atttttatcg ccctgaatat caaaaaagaa agaacgaaat ttgtcctttc tcgagcttag
      241 ctttttttca acccactaca gttgacaaag agccaatcta caagtcacta attttcatat
      301 aacaaaaaaa cgaacaggct ctttttctgt tattcagaag agaacttgtt cgtttttatc
      361 ttgattttct taaaaagctc tatatacata gggattttct ggtttatcga ctttggtaaa
      421 gctgtcgatt tccaaggttg gaaggttttg tttgagttct ttatggtagt gattgaccag
      481 ttttcctttg gctgtaatcc aggtgttgtt ttcatactgc cgggtattgc ccttggtcag
      541 caatccatag acaccagaat ctgcgataca gtggataatg ccgaatcgga acagaaattg
      601 actattggca tgactggggt cgttatagac aaagcctgta aactggattg tcttgccctc
      661 aaattcatct ggatagtcgt agatagcctc catgacttcc atatagtttt cattagtgat
      721 ctgaatacta tcttgggata agtatttatc cgccgccgtt cgcatttcct tttcataggc
      781 tgattttgaa aaataagaac tagtatctgg tttcaaatat tggcttgtcg tcccttcact
      841 tgtctgaata gctagatccg ttccttccga tagggggaaa tgataacctt tagcagaaac
      901 ggtctgagaa tccaagctaa cagttgggaa agttaagccg atgacaagtg gaatagccag
      961 aagagaaata ctcgtcatct tggccaatcg gctgttcaga tgactgtggg ttttgacttg
     1021 cttcatccag atatacaatt gaacaattgc caagataaag gaaagcacca tggagatata
     1081 ggccagatag gaatagtgca tgttgatgta ctggtttagt ttgcccgaca gatggaggta
     1141 aatagtcagt tcaaaatagc cagctaaaac taaaaatcga atcataggat gactccaatc
     1201 aagagagaat aggctaaaac gaccagagtc acaatcgtaa tgaagtgact aataaatcgt
     1261 gctttcaagt aatttttcat catgagaata tttttgatat ccagcattgg gccaatgacg
     1321 agaaaggcca gaactggtgc caaaccgaaa ctcgagagta gagaagcacc tataaaggca
     1381 tccgcctcac tacagagcga aagaagaaag gctaaaagca tcaagagcag gatagcaaaa
     1441 agaggagtcg cactgataga ggtcagaatc cgagtcggaa cgtagacctg tattatagaa
     1501 gcaaagaggc agccaaatac caaataacgc cccgtatcaa aaaattcatc aatggcctgc
     1561 acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag aaaaatcatg ctcatgacaa
     1621 gccagacgat tttctttctg aatcggttct tgccagaaaa atcctagaaa aattcctagt
     1681 attacagcca caagaatgga acccagagct cgtaataggg cgacatggaa ggagttgcca
     1741 aaggcagaat aggtcgcaaa aagaacaatg ggattgataa caggtgctgt cacaagaaaa
     1801 ggaacggccg tgtaacttgg aacctttttt tccagaaaac gattgatgat ggggacgatt
     1861 ccacattcac aagaagggaa aagtataccg acaaaggtcc caaaaaagat tctcccccaa
     1921 cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga tataaacttc aatcagccct
     1981 gagacaatgc ttcctatcag aacgaaggga agggcttcaa tgataatgga gagaaaaatg
     2041 gctccagttt gaagtacact agatgggagt tgttgaaaag cagtcatcta gtttttcttt
     2101 tctgctttga cttttttctc tttgtcatca gggaaagtaa cttgtttcaa atcagggagt
     2161 ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt ttgtaaattt gacagccata
     2221 ggtgtacctc gattctaaat tctacctcta ttatactatt attttgcgga aaagggaaaa
//
LOCUS       DAVLYR010000192         2252 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLYR010000192 DAVLYR010000000
VERSION     DAVLYR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.192"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            116..1462
                     /gene="gor"
                     /locus_tag="VYO63_001890"
     CDS             116..1462
                     /gene="gor"
                     /locus_tag="VYO63_001890"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1672754.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGLYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            1659..2195
                     /locus_tag="VYO63_001891"
     CDS             1659..2195
                     /locus_tag="VYO63_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1672755.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT          621 a          462 c          471 g          698 t
ORIGIN      
        1 gcttttttgc aattttttgt gcgtagcaga tttttgcaat caaatcaaaa aaagaataaa
       61 atataactat caaaaaagac gctctactat ttaaaagatt acaaaggagt tttcaatgag
      121 agaatatgat atcattgcta tcggtggagg tagcggagga atcgctacca tgaaccgtgc
      181 tggtgaacat ggagccaaag cagccgttat tgaggaaaag aaattaggtg gaacctgtgt
      241 caacgtcggt tgtgttccta aaaaaatcat gtggtacggg gcgcaaatcg ctgagacttt
      301 ccatcaattt ggagaagact acggctttaa gactactgat cttaactttg actttgcaac
      361 cctacgtcgc aatcgtgaag cctacattga tcgcgctcgt tcttcttatg atggtagttt
      421 taaacgcaac ggtgtagact tgattgaagg tcatgctgaa tttgtagatt ctcatactgt
      481 aagcgtaaat ggtgaactga ttcgtgctaa acatatcgtg attgctacag gtgcccatcc
      541 aagtattcct aatattcctg gtgctgagct aggtggctct tctgatgatg tatttgcctg
      601 ggaagaactt ccagagtcag ttgccattct aggcgctggt tatatcgccg ttgaattagc
      661 tggcgtactc cacacttttg gtgtcaagac agatctcttt gttcgccgcg atcgtccttt
      721 acgtggtttt gattcctaca tcgttgaagg tttggtcaag gaaatggaaa gaacaaactt
      781 accacttcac actcacaaag tccctgtcaa gttagaaaaa actactgacg gcattaccat
      841 tcatttcgaa gatggtacta gtcacacagc tagccaagtt atctgggcta caggccgccg
      901 tccaaacgtt aagggcttgc aacttgaaaa agctggagtg actctgaacg aacgtggctt
      961 tatccaagtg gatgaatacc aaaatactgt tgttgaggga ctctatgctc taggtgatgt
     1021 aacgggcgag aaagaactga ctccagttgc aatcaaggcc ggacgtaccc tatctgaacg
     1081 tctctttaac ggaaaaacta ctgcaaaaat ggattactca actattccaa ctgttgtctt
     1141 ttcacaccct gctatcggaa ctgttggttt gacagaagag caagctatta aagaatacgg
     1201 tcaagaccaa atcaaggttt ataaatcaag ctttgcatct atgtactctg cttgcactcg
     1261 caaccgtcaa gaatcccgtt tcaaactcat aacagctggt tcagaagaaa aagttgtcgg
     1321 acttcatgga attggctacg gcgttgatga aatgattcag ggatttgccg ttgctatcaa
     1381 aatgggagca accaaggctg actttgatgc aactgtggca attcacccaa ctgcatctga
     1441 agaatttgta accatgcgtt aatcgaaaca aaagaagctg atataaatat gtctgcttct
     1501 tttttaatga ttttcgaatc aacaaaaaaa ttatccaact tgactagtca cgaaaatcgg
     1561 ctgttacagt ttttgttacc ttgtttttaa aaaatggttg acatcgactc ctatatgagt
     1621 ataatagtta ctatacatta gaaaagaggt taactatttt gaaaaaagct cacgtttatg
     1681 ctatccctgc tattggggct gctctcattg ctgctttggc acaaatcagt cttccaattg
     1741 gacctgttcc cttcactctg caaaactttg caatcggctt gattgctact gtctttagac
     1801 cgagagaggc tgtactttct gctggactct atcttcttct aggtgctatc ggtcttcctg
     1861 tctttgcagg aggtggagct ggttttcagg ctttagttgg ccctactgca ggctatcttt
     1921 ggttttatct cgtttactct ggacttactt cctctctaac caacagcaag agtggtgttg
     1981 ttaagatttt tcttgcaaac ctcttgggtg atgcccttgt ctttgtcggc gggattctca
     2041 gcttgcattt cctagctgga atggcatttg aaaaagctct tgttgtgggg gttcttccct
     2101 ttatcattcc agaccttggc aaacttctgg ctattagttt tattagccgt cccctacttc
     2161 aacgccttaa aaatcaggct tactttacta actaaaaaag gataccgagt tatcatgact
     2221 caatatcctt ttcttttatt ttgaaaactt at
//
LOCUS       DAVLYR010000193         2210 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLYR010000193 DAVLYR010000000
VERSION     DAVLYR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.193"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            17..2122
                     /locus_tag="VYO63_001892"
     CDS             17..2122
                     /locus_tag="VYO63_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1672756.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     TKHLEADMEDGVLVIREKA"
BASE COUNT          681 a          440 c          512 g          577 t
ORIGIN      
        1 aaaagaggta aagaatatga ataacaactt taataatttt aataacatgg atgatttatt
       61 taaccaattg atgggtggta tgcgaggata cagttctgaa aatcgccgtt acttaattaa
      121 tggacgcgaa gtcacacctg aggaatttgc tcactatcgt gcgactggtc aattaccagg
      181 aaatgcagaa actgatgtgc aaatgccaca acaggcatca ggtatgaaac aagacggtgt
      241 ccttgcaaaa ctaggtcgaa acttaacagc agaagcgcgt gagggcaagt tggatcctgt
      301 tatcggacga aacaaggaaa ttcaagaaac atctgaaatc ctctcacgcc gcaccaagaa
      361 caatcctgtt ttggtcggag atgcaggtgt tggtaagaca gcagttgtcg aaggtctagc
      421 gcaagccatt gtgaacggag atgttcctgc tgctatcaag aacaaggaaa ttatttctat
      481 tgatatctca ggtcttgagg ctggtactca ataccgtggt agctttgaag aaaatgtcca
      541 aaacttagtc aatgaagtga aagaagcagg gaatattatc ctcttctttg atgaaattca
      601 ccaaattctt ggtgctggta gcactggtgg agacagtggt tctaaaggac ttgcggatat
      661 tctcaagcca gctctctctc gtggagaatt gacagtgatt ggggcaacaa ctcaagacga
      721 ataccgtaac accatcttga agaatgctgc tcttgctcgt cgtttcaacg aagtgaaggt
      781 caatgctcct tcggcagaga atacttttaa aattcttcaa ggaattcgtg acctctatca
      841 acaacaccac aatgtcatct tgccagacga agtcttgaaa gcagcggtgg attattctgt
      901 tcaatacatt cctcaacgta gcttgccaga taaggctatt gaccttgtcg atgtaacggc
      961 tgctcacttg gcggctcaac atccagtaac agatgtgcat gctgttgaac gagaaatcga
     1021 aacggaaaaa gacaagcaag aaaaagcagt tgaagcagaa gattttgaag cagctctaaa
     1081 ctataaaaca cgcattgcag aattggaaaa gaaaatcgaa aaccacacag aagatatgaa
     1141 agtgactgca agtgtcaacg atgtggctga atctgtggaa cgaatgacag gtatcccagt
     1201 atcgcaaatg ggagcttcag atatcgaacg tttgaaagat atggctcatc gcttgcaaga
     1261 caaggtgatt ggtcaagata aggccgtaga agctgtagct cgcgctatcc gtcgtaaccg
     1321 tgctggtttt gatgaaggaa atcgcccaat cggcagcttc ctctttgtag ggtctactgg
     1381 ggttggtaag acggagcttg ctaagcaatt ggcactcgat atgtttggaa cccaggatgc
     1441 gattatccgt ttggatatgt ctgaatacag tgaccgcaca gctgtttcta agctaattgg
     1501 tacaacagca ggctatgtgg gttatgatga caatagcaat accttaacag aacgtgttcg
     1561 tcgcaatcca tactctatca ttctcttgga tgaaattgaa aaggctgatc ctcaagttat
     1621 tacccttctc ctccaagttc tagatgatgg tcgtttgaca gatggtcaag gaaatacagt
     1681 aaacttcaag aacactgtca ttattgcgac ctcaaatgct ggatttggct atgaagccaa
     1741 cttgacagaa gatgcggata aaccagaatt gatggaccgt ttgaaaccct tcttccgtcc
     1801 agaattcctc aaccgcttta atgcagtcat cgagttctca caattgacta aggaagacct
     1861 ttctaagatt gtagatttga tgttggctga agttaaccaa accttggcta agaaagacat
     1921 tgacttggta gtcagtcaag cggctaaaga ttatatcaca gaagaaggtt acgacgaagt
     1981 catgggggtt cgtcctctcc gtcgcgtggt tgaacaagaa attcgtgata aggtgacaga
     2041 cttccacttg gatcatttag atactaaaca tctggaagca gatatggaag atggcgtttt
     2101 ggttattcgt gagaaagcct aagactcttc gaaaatcaaa ttcaaaccac gtcaacgtcg
     2161 ccttgccgta ctcaagtaca gcctgcggct agtttcctag tttgctcttt
//
LOCUS       DAVLYR010000194         2191 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLYR010000194 DAVLYR010000000
VERSION     DAVLYR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.194"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            27..299
                     /locus_tag="VYO63_001893"
     CDS             27..299
                     /locus_tag="VYO63_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV1672757.1"
                     /translation="MLDSGLVEEIRNQKGNLKYEYFLPIEKEGTILLIDQWINQETLD
                     KHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPESDKSFIDTGN"
     gene            489..1925
                     /locus_tag="VYO63_001894"
     CDS             489..1925
                     /locus_tag="VYO63_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1672758.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            1977..2191
                     /locus_tag="VYO63_001895"
                     /pseudo
     CDS             1977..2191
                     /locus_tag="VYO63_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
BASE COUNT          717 a          275 c          466 g          733 t
ORIGIN      
        1 atagtgcaat aaagtttgca agagaaatgc ttgatagtgg ccttgttgaa gaaattagaa
       61 atcaaaaagg taacttgaaa tacgaatatt ttttaccaat tgaaaaagag ggaactattc
      121 tccttattga tcagtggata aatcaagaaa ctttagataa acattatcaa tcaaagacaa
      181 tgcagaaaat cttagattta agaaaaaaat atcacttgca gatgcaagtt gaacgttata
      241 tagaagatga ttctgggatg ccagaaagcg ataagtcgtt tatagacaca gggaattaga
      301 attattgtga gagtttttgt agtttgaaag tattgaaaat cgtaaagata tcattacgag
      361 aatacaagaa gatattgttc tatttccatt aaaaattact tctttcctta acaataagga
      421 aaaagaatac gttttcattt tttggtttga ggtttataat aagagtatca aagtcaggag
      481 gaaaaaagat gactactttt aaagatagat ttttatgggg tggtgctgtt gctgctcatc
      541 aacttgaagg cggatggcaa gaaggtggca aaggaattag tgttgctgat gttatgactg
      601 ctggtcgtca tggagtagct cgtgaaatta cttcgggagt tttagagggt aaatattatc
      661 caaatcatga ggcgatagat ttttatcatc gttataaaga agacatagca ctttttgctg
      721 aaatgggatt caagtgcttc cgtacctcta ttgcatggac acgtatcttt ccaaaaggtg
      781 atgagttaga gccgaatgaa gaaggattac agttttatga taatcttttt gatgaatgct
      841 taaagaatgg tattgaacct gtcatcactc tatctcattt tgaaatgcct tatcacttag
      901 tgaccgaata tggtggttgg aaaaatagga aattgattga tttctttgct cgttttgcag
      961 aagtcgtatt taaacgttac aaagataagg ttaaatattg gatgactttc aatgaaatca
     1021 ataatcaagc gaattatcag gaagattttg caccatttac taactcaggt attgtatatg
     1081 aggaaggtga taatagagaa gcaattatgt atcaagcagc acattacgaa ctagttgctt
     1141 ctgcacgagc tgtaaaaatt ggtcatgaga ttaatccaga ttttcaaata ggttgtatga
     1201 ttgcgatgtg tccaatttat ccagctactt gcaatcctaa ggatatctta atggcaatga
     1261 aagctatgca gaagcgttat tattttgctg atgtgcatgt tttaggtaaa tatcctgagc
     1321 atattttcaa gtattgggaa cgaaaaggta tttcagttga ttttactgac caggataaag
     1381 aagatttact tggtgggact gtagattaca ttggtttcag ttactatatg tcctttgcta
     1441 tagactctca tcgtgaaaat aatccttatt ttgattatct tgaaacagaa gatttagtga
     1501 aaaataatta tgttaaggct tctgaatggg agtggcaaat tgatccagaa ggtttacgtt
     1561 atgcgttaaa ttggtttaca gaccactatc acttaccact ctttattgtt gaaaatggtt
     1621 ttggagctat agatcaagtt gcagcagatg gtatggtaca tgatgattat agaattgaat
     1681 atctaggtgc ccatattcgt gaaatgaaaa aggctgtagt tgaagatggt gttgatttaa
     1741 tgggttatac tccatgggga tgtattgatt tggtttcagc tggtaccggt gaaatgcgga
     1801 aacgttatgg ttttatttat gtagataaag atgacaatgg gaagggaagt tataatcgtt
     1861 ccccgaaaaa atcttttggc tggtataagg aagttatttc atctaacggt gaatcagtag
     1921 aatagtataa acaaactgag attattctca gtttgtttag catattggga tgacatatga
     1981 ttggaagtat tgcagcaatt ttaacaacat ttgcttttta ccacaagttt ttcgtatagt
     2041 taagacaaag gacacaggat ctattgctct aggtatgtat gttatgcaag ttattgatat
     2101 tgcattgtgg cttgctcatg gtattcgtat aggagacttg cctttgattt tagcaaatag
     2161 tgtttcattt ctactctcag gaataatttt a
//
LOCUS       DAVLYR010000195         2180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLYR010000195 DAVLYR010000000
VERSION     DAVLYR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.195"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            247..2130
                     /locus_tag="VYO63_001896"
     CDS             247..2130
                     /locus_tag="VYO63_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1672759.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFIIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGDKVKVGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
BASE COUNT          598 a          435 c          477 g          670 t
ORIGIN      
        1 tttttacgaa aatgtgaaag ataatgaaat aaaagacttt ttaaccaagg tttcaaactt
       61 atttttcaag gaattcctga tgaaacagtc aaaaacaatc aatctaaagc ttgcccacta
      121 agtggataga atatttttct gcaaaacgct tgacatattt ttaaaacatg ataaaataat
      181 agtcgtaagg gcgaataaaa tcgctttcaa acaagaacaa aatgttatat aaggagattt
      241 ttgcaaatga acaatcagga aattgcaaaa aaagtcatcg atgccttggg cggacgtgaa
      301 aatgtcaata gtgttgccca ctgtgcgact cgtctacgtg tcatggtcaa agatgaagag
      361 aaaatcaata aagaagtgat tgagaacttg gaaaaagttc aaggtgcttt ctttaactca
      421 gggcaatacc aaattatctt tggtacaggt acagttaaca aaatgtacga tgaagttgtt
      481 gtacttggct taccaacatc atctaaggat gacatgaaag cagaagttgc taaacaaggg
      541 aactggttcc aacgtgctat ccgtactttt ggtgatgttt tcgttccaat catcccagtt
      601 atcgtagcga caggtctctt catgggtgtg cgtggtcttt tcaacgctct tgaaatgcca
      661 cttccaggtg actttgcaac ttacacacaa atcttgacag atacagcctt catcatcttg
      721 ccaggtttgg ttgtgtggtc aaccttccgt gtatttggtg gaaatcctgc cgttggtatc
      781 gttcttggta tgatgcttgt ctctggctca cttccaaacg cttgggcagt tgctcaaggt
      841 ggtgaagtaa cagcgatgaa cttctttggt ttcatccctg ttgttggttt gcaaggttcc
      901 gttcttccag ccttcatcat cggggttgtc ggagctaaat ttgaaaaagc tgtccgcaag
      961 gttgttccag atgtcattga cctcttggta acaccattcg tgacactttt ggtcatgtct
     1021 atccttggac tctttatcat tggaccagtt ttccacgttg ttgaaaacta catccttatt
     1081 gctacaaaag cgattcttag catgccattt ggccttggtg gtttcttgat tggtggggtt
     1141 caccaattga tcgtcgtgtc aggtgtgcac cacatcttca acttgcttga agtgcaatta
     1201 cttgctgctg accatgctaa cccattcaac gctatcatca cagctgctat gacagctcaa
     1261 ggtgctgcta ctgttgcggt tggtgttaaa acaaaaaatc caaaactgaa aacacttgct
     1321 ttcccggctg ctctttctgc cttcctaggt attacagagc ctgctatctt cggggtgaac
     1381 ttgcgcttcc gtaaaccatt cttcctttca ttgattgctg gtgcaatcgg tggtggattg
     1441 gcttctatcc ttggacttgc tggtactggt aatggtatca ccatcatccc tggtacaatg
     1501 ctttatgttg gtaacggaca acttccacaa taccttctta tggtagctgt atcatttgcc
     1561 cttggttttg ctcttactta catgtttggt tacgaagatg aagtagacgc aactgcagct
     1621 gcaaaacaag ctgaagtggc tgaagaaaaa gaagaagttg cgccagcagc tcttcaaaat
     1681 gaaacacttg taactcctat cgtcggtgat gttgtcgctc ttgctgatgt caatgaccca
     1741 gtcttctcaa gtggagctat gggacaaggt atcgctgtga aaccaagcca aggcgtggtc
     1801 tatgcaccag ctgatgctga agtttcaatt gcctttccaa cagggcacgc ttttggtttg
     1861 aaaacaagaa atggtgctga agttttgatt catgttggta ttgatactgt atctatgaac
     1921 ggtgacggtt ttgaaacaaa agttgctcaa ggtgataagg tgaaagttgg cgatgttctt
     1981 ggaacatttg actcaaacaa aatcgctgca gctggacttg atgatacaac aatggttatc
     2041 gttacaaata caggtgacta cgcttcagta gctccagtcg caacaggttc agttgctaag
     2101 ggggatgctg tgatcgaagt gaaaatctaa tcaatcctct ctaatgtgaa aacgaacaaa
     2161 tgacatattt gttcgttttt
//
LOCUS       DAVLYR010000196         2167 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLYR010000196 DAVLYR010000000
VERSION     DAVLYR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.196"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..1463)
                     /gene="pfbA"
                     /locus_tag="VYO63_001897"
     CDS             complement(<1..1463)
                     /gene="pfbA"
                     /locus_tag="VYO63_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1672760.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYSGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVS"
BASE COUNT          676 a          410 c          302 g          779 t
ORIGIN      
        1 tctgatactt ttcctaaata ttctgcacta tcttttgcaa ctcgtatcgc ttttgtttta
       61 ggatcggcaa tattaaatat attttcggcg ataaccacct gtttatttaa atctaatagg
      121 tctttagtgt ttttatagct ataagcattt actaaagctg ctccgctttc tcgataatgt
      181 acactctctc ctttaacttt cttatcaaag cgatttcctt tgattaatac atcagtgaat
      241 cctgtaaaac gtacacctga atacatcatg ttatcaaaat gattatttaa aattttaata
      301 ttagaggggt tctgtgtcga caatgtttga tagtgtgtgc caattgctgt tactaattcc
      361 ccagatttat cacttttgcc aaaataggaa ttttgaatag tcacattttc agattttttc
      421 ccatcatcat tcaaggcata aggaaaacct tttctagtta atggttcaat ctgaatgctc
      481 tccttactta tgatttgccc atccttcatc gttttgggta aggcttgccc aagaaaacga
      541 gaattatcaa ctaatacatt tttcgaacct gcaatttgaa tagcatgccc ttgataacta
      601 tccttgaatg ttacattttt tatagttacg ttatttgaat tcccaatagc aaatgcacct
      661 gaagaattta taagtggtag attttttgct ttagttcctt cttcattcaa agcaccgttc
      721 atatcaatcg taccaccaga ataactaata tcttctgttg ggccccattc tacttgcgca
      781 ccatcatccg taaataaacc tgtcataaaa acaatggaag ggtgattctt aatatttata
      841 ccatttagaa ttgtagcttt ctcattcaat tctaagtgtg tatgactttt taaaaaaaca
      901 atttctttta ctaaataagt tccttcagga aaatatacat ttcctccacc tagcccttga
      961 gctgcagcat ctattgcatc ttgaattgct tgtcgatcat catgaatccc atcacctacc
     1021 gcaccataat cttttacact taaagcagta tcttcaaatt cttttttgat aattctgtca
     1081 cttataacat cttccttata actcttattt tcattagtag atgttactgt agattcaact
     1141 gttctgttgt ctaattgtga attggaacga atagtaccat tagagctgat actattaaca
     1201 cttaacgaat tatctaaacc attagtggtt gaattagagg caatcatttc agcagaacga
     1261 ttctgattaa cagttggtga attatctaaa tcattagtaa ttgcattaga agactctttt
     1321 gtagccatcg gtgaagaact agtaacaact tcatccgata aaataccttg gcttcccaaa
     1381 attgaaattg ccaatagtac cgagcaagtc cctaccttta atttacgaat actgaatacc
     1441 tcattaggaa caaaatattt catcagaacc tccaattttt ttactatttt taacaattga
     1501 ttattacttt tcttaaaata gatagaatga caccgctgtc attatcttta tcatatattt
     1561 tccaaattga attgtcaagg aataacacaa gaaaatatta aaatttatac tcttcggaaa
     1621 tctcttcaaa ctaggtcagt gccgccttgc cctaagtcta ggttactgac ttggtcagtt
     1681 ctatagtaga cggaaactat aatagtacac ctctacttct aaaacattgt tagaaatcga
     1741 tttgactgtc ccgatcgatt tgtcctattc ttatttcatt ttactatatc tacaacctca
     1801 aagcagggtt ttgagctatc tatggccaac ttcctagttt gctctttagc cttccattca
     1861 aaagtgatgc tatctttctt agcttgaata taaaatatcc catttactat atttaaattt
     1921 gttgatatgt tgtaaattat aaatgaaaga acaaatacct tgtcatacaa gcattcgcca
     1981 gggatattta catgaatatc gctcatatgg cggattgaaa ctaaaacact acaatatatt
     2041 tcttaaatag accttctata aaagctagat ttaacctcga taaaaataga tgcagtgctt
     2101 ctaaaatact ggaatagggc atataggtaa tcgtagacaa gtgttttata gattgtctcg
     2161 cgggaga
//
LOCUS       DAVLYR010000197         2159 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVLYR010000197 DAVLYR010000000
VERSION     DAVLYR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.197"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..895)
                     /locus_tag="VYO63_001898"
     CDS             complement(<1..895)
                     /locus_tag="VYO63_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1672761.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(898..2115)
                     /locus_tag="VYO63_001899"
     CDS             complement(898..2115)
                     /locus_tag="VYO63_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1672762.1"
                     /translation="MGNNKALVSIRDGITLTIPLLLIGSLLMVIASFPIPGWEKYLGD
                     IGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGIVSLSSFITVTPFIRG
                     EAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMPDGVPPAVSKSFSAII
                     PGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNVIGLLILIFLNSSFWF
                     VGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFMDNFVFIGGGGATIGL
                     VLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIVLNILLLVPFILAPMF
                     NLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLLQIVLIVLDVLLYLPF
                     VIAIEKRFKLLED"
BASE COUNT          749 a          491 c          300 g          619 t
ORIGIN      
        1 acctatttac tgacctagcc aagcgagata ctgtatgtct cactcctcta attgccttgc
       61 tcaatgcata aatattatcg actgttgcca gtcccccata tcctgcgtca cctatatgtt
      121 gaatatcaac tccacaaatt ttatttctaa gtgcaatttc cttgatagta tctgtatcag
      181 atgtttcttg gctagtacca atagcactca atacaagacc acccttacta tgaacgagat
      241 caacgacttc acgcaactct tggtcatgaa aggctggaac ggttccaact gctggaacaa
      301 gtatcacatc cgcacctgct tccaacaatt gctctgcgac agaaagctct gccacaggct
      361 cattcactcc tgccccgtgc attttaccag ctataatcaa acctgagaaa ttctctttag
      421 cagattgaac tgctttgata atctcttgat tgctaactcc tgttccagga tttccagtca
      481 aacagacaaa gtcaaaacct aactcctcta ttcgattcaa tgtttcaaca ctagcaacac
      541 gacctgcaac aatttcctgt gtttcctcta gcatctttgc agatgggtca atcggttcca
      601 aatttacacc aattggacaa gcaacaagct ggtgtaagcg atgaataaca tcatcacctg
      661 aactatccaa agcataaatt ttaggctcaa aaacatcaac acaattcaat aaaatcatat
      721 cagctccaaa agctctagca atttcagaat tagttatatc cccaacaaat gtctcacgag
      781 taactacatt ttcagataga acaactctac cttcacttgc taaaatactt tgttttaatt
      841 cttcagcatt catctgtaat atctcagatg gatttgcact aattaaacgt ttcattctca
      901 atcctccaat aacttaaacc gtttttcaat agctattaca aatggtaaat aaagtaagac
      961 atccaaaaca atcaatacaa tttgtaataa tgaaccagaa atacttcctg tagcaagtaa
     1021 accacttata acaggtggca tagtccaacc tggatctgta taagttagcg gaaccaaacc
     1081 tgatgccatt gctccccacg ctactagtaa gttaaacatt ggagctaaga taaatggaac
     1141 tagtaacaaa atgttcaaaa caataggaac gccaaacatt gctggttcat ttatattaaa
     1201 taatccaggt ataacagtta taggtgccaa agtttttagt tgtttactag ctttcttctt
     1261 atgtgctaaa tatcctaacg caagaactaa acctatggtt gcaccaccgc cacctatgaa
     1321 tacaaaatta tccataaata cgctagtaaa aatatttgga agagtttctc cagtttgata
     1381 agctacttta tttgcatcta gattagctaa ccataacggt ttcataactg cattaacaac
     1441 atttcctccg tgtaatccta caaaccaaaa actactatta aggaaaatta agataagaag
     1501 accaataaca ttatttccta aaagtccaag tggacctccc aaagctactt gagcaatttc
     1561 atgaagatta ggtaaactta atttatctaa agttgcataa acaatcaacc aaccaacaat
     1621 agtcactgca ccaggaataa tggcactaaa gcttttagat actgctggtg gaacaccatc
     1681 cggcattttt atctgtatat tatgatttat aaaccattgg tagatatagc cattaatcaa
     1741 tcctaaaatc atagcaacaa ataaaccttt tgatgccatg aaagcggtag gcattcctgc
     1801 tccagcctct cctctaatga aaggcgtaac tgtaataaaa gatgataatg acacaatacc
     1861 agcaggaata ccatcaactt tgtattgtct agccataaag taagcgatac caaaactagc
     1921 tacaagacca agtaatccaa aactactatc aacacccttc cataaatagt cagcaacacc
     1981 aatatcaccc agatattttt cccatcctgg aataggaaaa cttgcaataa ccattagtaa
     2041 tgaacctatc aacaataaag gtatagttag agtaataccg tctcgaatag aaaccaacgc
     2101 tttattatta cccaatctag ccgctactgg aagtaacttt tcttgtaata atttttcca
//
LOCUS       DAVLYR010000198         2120 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVLYR010000198 DAVLYR010000000
VERSION     DAVLYR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.198"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(87..1283)
                     /gene="tuf"
                     /locus_tag="VYO63_001900"
     CDS             complement(87..1283)
                     /gene="tuf"
                     /locus_tag="VYO63_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1672763.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          633 a          416 c          421 g          650 t
ORIGIN      
        1 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct gacttaaata
       61 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
      121 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
      181 atcaactcaa catcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
      241 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
      301 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
      361 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
      421 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
      481 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaacg
      541 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
      601 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
      661 cgttctggtt ctgggatata ctcatcaact gtgttcatca attccataac gatgtcttcg
      721 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
      781 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
      841 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
      901 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
      961 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
     1021 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
     1081 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgataga cgcatagtct
     1141 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
     1201 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
     1261 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttttatagat
     1321 agacagtagg caatacagtc taactttcct tactatttta tcaaatttca gctaaattgc
     1381 aagtgtttta caacgttttt gtgaattatt cttggtaaaa atttaactaa ctaatagaaa
     1441 gtactaaata gacttatcct tcaaggagtt ttcagcatta tccatccact cacttgctga
     1501 aaatgtaaac agtctctggt ttatatagta aaatgaaaca agaatagggc aaatcgatca
     1561 ggacagtcaa atcgatttct aacaatgttt tagaagttaa ggtgtactat tctagtttca
     1621 atctactata caacctcaaa actgtgtttt gaacaacctg cggctagctt cttagtttgc
     1681 cctttgattt tcattgagta taaaattgca gataaaacga taacaatcat aggactcagc
     1741 agagtcaatg taggtcgaag aatagccccc ctaatcgctt attttatagc aaaatgaaat
     1801 agaaactgaa cattttgttg aggaaggtaa atcaattcct cacaatgttt gagaaagaag
     1861 ggaggactat tccaacttca acccactata aagcagactt acaagatagg gaacccatct
     1921 ccgataatat atagtagaat gaaaactgca caatttgatt ggagaattca aaccaatttc
     1981 taacaatgtt ttagaaacaa gggtctatta tttcaactgc aacctactat agcccattga
     2041 agctggaata gcccactgag acttctaaac cactgctgga gaggagtttg tttagagttt
     2101 ctttcatttc cctaaaaaat
//
LOCUS       DAVLYR010000199         2107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVLYR010000199 DAVLYR010000000
VERSION     DAVLYR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.199"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..456)
                     /locus_tag="VYO63_001901"
     CDS             complement(<1..456)
                     /locus_tag="VYO63_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1672764.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFA"
     gene            complement(469..>2107)
                     /locus_tag="VYO63_001902"
     CDS             complement(469..>2107)
                     /locus_tag="VYO63_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1672765.1"
                     /translation="DVANYYKEFVKLGNFESDSVVQGHKGNILERIIEYIAGSMTPII
                     PAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAYSASQKLKVT
                     STLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVIPASYGSSVVPILIMVWLMKYIEKM
                     IAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMYDVAGWLTLA
                     ILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAASMAVALKSKN
                     NNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFAGLTSVKAYL
                     FAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEESSSNLEVKAG
                     VSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFDGKITNIFPT
                     KHAIGLKSDESVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEMDLNLIKTKG
                     YETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
BASE COUNT          726 a          436 c          297 g          648 t
ORIGIN      
        1 agcaaatttt tcataaaaat gaatcattct cctatcaatc catccatgat attttcttgc
       61 taaatataat ggagtctcat agtgtgaaag agttacaagt ggttctatcc cgtgagcatg
      121 tagttcatca aacaattcat cataatattt caacccagct tcgttaggtt cttcctcatc
      181 tccttttgga aaaattctac tccatgcaat agaagtacga aaaacattaa agcccatttc
      241 agaaaacaag gatatatctt ccttatattt atgataaaaa tcaataccta tcaattttaa
      301 gttatcttct gtaggatttt ctgttgcttc tcctaatcca cctttgggta acacatcctg
      361 aactgataag cccttaccat cttcattata tgctccctct acttgattag ctgcaacagc
      421 tccaccccaa agaaaatcat ctggaaaaat ggtcataact ttcctccatt ataatattac
      481 cagtaattcc ttagaatgct cgattgtctg attattaggt aatactaata catctagaaa
      541 atcattggta ttcgttacaa ttactggtgt aactgtttcg tagcctttag tcttgattaa
      601 attcaagtcc atttcaaaaa tcaactgatt tttgaaaact ctgtctcctt cttctacatg
      661 actaataaaa ccttgacctt ttagctcaac agtatctaat ccaatatgaa ttagtaactc
      721 aacactctca tcactcttca atccaattgc gtgcttagtc ggaaaaatat ttgtaatttt
      781 cccatcaaat ggtgcataaa ccttaccttc acttggaata atcgctactc cgtctccaat
      841 tagtttatct gaaaatgttt tatcctggac atcgcttaac ggaatgattt ctcctgatat
      901 aggagaaaat atcatttttt tatttgaaac tccagcttta acttctaaat tgctagaact
      961 ctcttcttca tcgattccaa atatataagc taatacaaag gtaataacaa ccgaaataac
     1021 cgccacaatt aaagcattta caatatttga tggtacatca gaataaataa attgaggcaa
     1081 cgctatcaaa gatgggacag caaatagata cgctttaaca ctagtaagac ctgcaaataa
     1141 tcccgctaat ccaccaccaa tcatagctgc ataaagcggt tttttatatt ttaaagtcac
     1201 accatataat gcaggttcgg taatccctgc aagtaaggct gagaaacctg ctgcaaaagc
     1261 aatttgtttt gtattattat ttttactctt taatgcaaca gccatcgaag cagccccttg
     1321 agctaagttt gaccctaaca ttgctggaag aattaatacg tctggagtag caatagatgc
     1381 cgccaaaaaa ataggtgcaa aagcccaatg cattccagtc ataacaataa atggcataat
     1441 agcaccaaga atagctaatg taagccatcc agctacatca tacatttgcc caactagatt
     1501 tgataatcct tcaccaacaa ttactccaat aggcccgact acaactaagg caatacagct
     1561 tgatactaat aatactagcg taggttgcaa aaaactctta gtaacagctg gtgttaattt
     1621 agcaatcatt ttttcaatat atttcatcaa ccaaaccata ataagaattg gaacgactga
     1681 cgaaccataa ctagctggta tcacaggtac accaaataaa ctaagaggat tccctgattg
     1741 caccatttga acaaaatttg gatggagaag tacacctgct acagacatag ctaatgtaga
     1801 tgttactttt aatttttgtg atgcagaata agctaataac agcggtaaga aataatatgg
     1861 agcatcccca aaaaatgtca aaaaagcaat agtctgagaa tctgattgca atataccaag
     1921 cattggtaaa ataattacca agactttcag catacctccc cctaacattg ctggaatgat
     1981 tggagtcatg gaaccagcga tatactcaat gattctttct aaaatattcc ctttgtgccc
     2041 ttgaacaact gaatcggatt caaaattgcc aagtttaacg aattctttat aataattagc
     2101 tacatca
//
LOCUS       DAVLYR010000200         2099 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVLYR010000200 DAVLYR010000000
VERSION     DAVLYR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.200"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(233..544)
                     /locus_tag="VYO63_001903"
     CDS             complement(233..544)
                     /locus_tag="VYO63_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672766.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCSFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(559..1845)
                     /locus_tag="VYO63_001904"
     CDS             complement(559..1845)
                     /locus_tag="VYO63_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1672767.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFATKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDVY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          641 a          454 c          362 g          642 t
ORIGIN      
        1 agacgcaatt ctaatctttt agactttgca taataactgt taaacagttg aaaattaacc
       61 cagttttaac aagtagtcaa aataccagat aaaggaaaaa agtctttaaa atcttaaaaa
      121 cgcatagtat caggtattga acaatcttga tattatgcgt tttattgtgg gaagatttac
      181 ttcattttct cttggaattg agttttctcc tacttcaaaa ctagatgatt tgctatttaa
      241 aatagcgttc cttatgattt tggcaacctg gattaaagcg agaaaaacaa aaggagcaac
      301 ttaaactttc tttatattgc ttatatgtta gtagttttag acaaacacca cataaaatag
      361 gcttatcctg tatcaaagat aagggatacg gctcaaaagg gtgattttct aatgaatcat
      421 gacaccgata acaagcatag tactttttac aatcatagca ttgtaaagca ataatatcca
      481 acttgctatg gtaatgaatg catcttgttt gcttatccac taagaaaccc tgtgcctcta
      541 tcatttcctt ttcctttctt aagcacgaac tgtgacgctg gttccatctt ccttataaaa
      601 attgatgagt ccttctttta atgcacgaac catgtctcct gattgaacgg gttgtgggac
      661 tttaatagtc aatagttcca ttggatttgg agcgcggtcg attttattgc ctttggcatc
      721 atgcaaatct tcaatatagg tttcaaaatg acggaaacct ggaccataaa actcaacttg
      781 gtccccttcg ttaatgacgt ttcgttgacg aatggttgct gtttgtgccg catcatcata
      841 agaaaccact tcagcgacaa acttgtactc aggaatttta cggcgagcac caaacaactg
      901 ctcattttca gatggtatac cgtagtagaa acctgttgcc aattcacgtt gggcaacctt
      961 ccacatctcg tccaccaagt cttgtttgat agcttcaaac ttttcaggac tttcaagata
     1021 gacatccaca gccgccttgt agcagttggt tactgttgat acgtagtgaa tagacttcat
     1081 acgtccttcg atttttagac tgtccacacc attttcaatc atatctggaa tatggtcaat
     1141 catagacatg tcaacggctg acattgaaaa ttcttctgga atctcaccct gcaaactctt
     1201 acgttctttc ccaaatggca tatcgtaaag gtcgtatttc caacggcatg actgagaaca
     1261 tccaccacgg ttggcatcac gcatactcat gtggtttgaa agagtacaac gtccagagta
     1321 tgaaatacac atagctccat ggacaaaggc ttcaatttca acatctgtac gtttgcggat
     1381 ttcagctaat tcttccattg aaacctcacg cgctaaaacg acacgagtca agcctagctc
     1441 tttccagaac tcaagggttt catagttagt ggcactggct tgggtagaaa ggtggatttc
     1501 aaggcctggt gcttcagtcg ctgcaatcat aatcaaggct gggtcagata cgataactgc
     1561 tgcaatcccg atatcacgca gtttacggaa ccactcacca gcaccagctt catttccttc
     1621 gtgcataacc atattagccg ctacatagac cttggcacca tacttggtcg caaactgcac
     1681 gccttcttcc atctgttcga aagtaaagtt tcccgcacgg ctacgaagac cataggcctg
     1741 accaccgata aagacagcat ctgctccata ctgaacagct acctttagct tctctaaagt
     1801 ccctgcaggt gataaaacct caggacgttt taatgttttt gtcatgattt ctcctatttg
     1861 caatataaaa gtttgacatt tttccttctc attttatagt aaataaagga taaaatcaag
     1921 cctagacgga ttgttttgac aattctaatc ttttagactt tgcataataa ctattaaaca
     1981 gtcgaaaatt accccagttt taacaagtag tcaaaaagtc taaaaaagta aaaaccagta
     2041 tcccaagata ctgactcgta aaaatatata aattagtcct tagatgacta ctcccactc
//
LOCUS       DAVLYR010000201         2088 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVLYR010000201 DAVLYR010000000
VERSION     DAVLYR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.201"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            54..169
                     /gene="rrf"
                     /locus_tag="VYO63_001905"
     rRNA            54..169
                     /gene="rrf"
                     /locus_tag="VYO63_001905"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            174..246
                     /locus_tag="VYO63_001906"
     tRNA            174..246
                     /locus_tag="VYO63_001906"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:207..209,aa:Val,seq:tac)
     gene            249..321
                     /locus_tag="VYO63_001907"
     tRNA            249..321
                     /locus_tag="VYO63_001907"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:282..284,aa:Asp,seq:gtc)
     gene            346..418
                     /locus_tag="VYO63_001908"
     tRNA            346..418
                     /locus_tag="VYO63_001908"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:379..381,aa:Lys,seq:ttt)
     gene            424..505
                     /locus_tag="VYO63_001909"
     tRNA            424..505
                     /locus_tag="VYO63_001909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:458..460,aa:Leu,seq:tag)
     gene            524..596
                     /locus_tag="VYO63_001910"
     tRNA            524..596
                     /locus_tag="VYO63_001910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Thr,seq:tgt)
     gene            619..690
                     /locus_tag="VYO63_001911"
     tRNA            619..690
                     /locus_tag="VYO63_001911"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:651..653,aa:Gly,seq:gcc)
     gene            698..783
                     /locus_tag="VYO63_001912"
     tRNA            698..783
                     /locus_tag="VYO63_001912"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:732..734,aa:Leu,seq:taa)
     gene            793..866
                     /locus_tag="VYO63_001913"
     tRNA            793..866
                     /locus_tag="VYO63_001913"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:827..829,aa:Arg,seq:acg)
     gene            882..955
                     /locus_tag="VYO63_001914"
     tRNA            882..955
                     /locus_tag="VYO63_001914"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:916..918,aa:Pro,seq:tgg)
     gene            961..1034
                     /locus_tag="VYO63_001915"
     tRNA            961..1034
                     /locus_tag="VYO63_001915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:995..997,aa:Met,seq:cat)
     gene            1048..1121
                     /locus_tag="VYO63_001916"
     tRNA            1048..1121
                     /locus_tag="VYO63_001916"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1082..1084,aa:Ile,seq:cat)
     gene            1132..1221
                     /locus_tag="VYO63_001917"
     tRNA            1132..1221
                     /locus_tag="VYO63_001917"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1168..1170,aa:Ser,seq:tga)
     gene            1232..1305
                     /locus_tag="VYO63_001918"
     tRNA            1232..1305
                     /locus_tag="VYO63_001918"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Met,seq:cat)
     gene            1309..1381
                     /locus_tag="VYO63_001919"
     tRNA            1309..1381
                     /locus_tag="VYO63_001919"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1342..1344,aa:Phe,seq:gaa)
     gene            1401..1471
                     /locus_tag="VYO63_001920"
     tRNA            1401..1471
                     /locus_tag="VYO63_001920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1433..1435,aa:Gly,seq:tcc)
     gene            1507..1580
                     /locus_tag="VYO63_001921"
     tRNA            1507..1580
                     /locus_tag="VYO63_001921"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1541..1543,aa:Ile,seq:gat)
     gene            1587..1674
                     /locus_tag="VYO63_001922"
     tRNA            1587..1674
                     /locus_tag="VYO63_001922"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1621..1623,aa:Ser,seq:gct)
     gene            complement(1807..>2088)
                     /locus_tag="VYO63_001923"
     CDS             complement(1807..>2088)
                     /locus_tag="VYO63_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672768.1"
                     /translation="KEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYS
                     NAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT          497 a          431 c          556 g          604 t
ORIGIN      
        1 attgttttct tggtttgaat agatattcaa ttttgagtag gtattactca gagttaagtg
       61 acgatagcct aggagataca cctgtaccca tgccgaacac agaagttaag ccctagaacg
      121 ccggaagtag ttgggggttg ccccctgtga gatagggaag tcgcttagct ctagggagtt
      181 tagctcagct gggagagcat ctgccttaca agcagagggt cagcggttcg atcccgttaa
      241 ctcccaaagg tcccgtagtg tagcggttat cacgtcgccc tgtcacggcg aagatcgcgg
      301 gttcgattcc cgtcgggacc gtttaaggta acgcaagtta ttttagactc gttagctcag
      361 ttggtagagc aattgacttt taatcaatgg gtcactggtt cgagcccagt acgggtcata
      421 tatgcgggtt tggcggaatt ggcagacgca ccagatttag gatctggcgc ttaacggcgt
      481 gggggttcaa gtcccttaac ccgcattaag atataacaaa tgagccggct tagctcagtt
      541 ggtagagcat ctgatttgta atcagagggt cgcgtgttca agtcatgtag ccggcatttt
      601 tagatagaag aaaacagtgc gaacgtagtt cagtggtaga acaccacctt gccaaggtgg
      661 gggtcgcggg ttcgaatccc gtcgttcgct tagagaggcc ggggtggcgg aactggcaga
      721 cgcacaggac ttaaaatcct gcgattggta acgatcgtac cggttcgatt ccggtcctcg
      781 gcataatatg gagcaccctt agctcaactg gatagagtac ctgactacga atcaggcggt
      841 tagaggttcg actcctctag ggtgcatttt tctatttaac tcgggaagta gctcagcttg
      901 gtagagtact tggtttggga ccaaggtgtc gcaggttcga atcctgtctt cccgattcat
      961 ggcggtgtag ctcagctggc tagagcgtcc ggttcatacc cgggaggtcg ggggttcgat
     1021 ccccttcgcc gctataatga tcttgtcgga cctttagctc agctggttag agctctcggc
     1081 tcataaccga gtggtcgtag gttcaagtcc tacaaggtcc atttgaatat tggaggatta
     1141 cccaagtccg gctgaaggga acggtcttga aaaccgtcag gcgtgtaaaa gcgtgcgtgg
     1201 gttcgaatcc cacatcctcc ttttatatta acgcgggatg gagcagctcg gtagctcgtc
     1261 gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaataagg ctcggtagct
     1321 cagttggtag agcaatggat tgaagctcca tgtgtcggcg gttcgattcc gtctcgcgcc
     1381 attttattga tagtatttat gcgggtgtag tttagtggta aaactacagc cttccaagct
     1441 gttgtcgcga gttcgattct cgtcacccgc tttgaacttt gttcaaatta ccaagttttt
     1501 taacttgggc gcgtagctca ggtggttaga gcgcacgcct gataagcgtg aggtcggtgg
     1561 ttcgagtcca ctcgtgccca ttaattggag aattactcaa gaggctgaag aggacggttt
     1621 gctaaatcgt taggtcgggt aactggcgca agggttcgaa tcccttattc tccgttttaa
     1681 tgagagtttg taactctctt ttttattttt agagacaata aagcttcaga tccgtaaaac
     1741 ttaattgaat tactaatctg aagttatttt taggctcttt gtcaactgta gtgggttgaa
     1801 aaaaagctaa gctcgagaaa ggacaaattt tgtcctttct tttttgatgt tcaaagcgat
     1861 aaaaatcctt tttttgaagt tttcaaagtt ccgaaatcca aaggcgtttc gtttgataag
     1921 tttgatgaga ttatttgtcg cttccagttt ggcgttagaa tagtgtagtt gaagggcgtt
     1981 gacaatcttt tctttatctt tgaggaaggt tttaaagaca gtctgaaaaa taggatgaac
     2041 ctgcttcaga ttgtcctcaa taagcccgaa aaatttctct ggttcctt
//
LOCUS       DAVLYR010000202         2080 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVLYR010000202 DAVLYR010000000
VERSION     DAVLYR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.202"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            40..491
                     /locus_tag="VYO63_001924"
                     /pseudo
     CDS             40..491
                     /locus_tag="VYO63_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            656..955
                     /locus_tag="VYO63_001925"
     CDS             656..955
                     /locus_tag="VYO63_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1672769.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1064..>2080)
                     /locus_tag="VYO63_001926"
     CDS             complement(1064..>2080)
                     /locus_tag="VYO63_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1672770.1"
                     /translation="LPSRTILLNWLAQYRKNGYTIVEKTKGRVPESGECHPKKVKRTP
                     IEGGKRERRKTEIVQELMTEFSLVLLLKSIKLARSTYYYHLKQLDKTDKDQELKAEIQ
                     SIFIEHKGNYAYRRIYLELRNRGYLVNHKRVQGLMKVLNLQAKMRQKRKYSSHKGDVG
                     KKAENLIQGQFEGSKTMGKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSP
                     NLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDN
                     GMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRT
                     KSFG"
BASE COUNT          646 a          403 c          360 g          671 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagt gaatacagac tatattactc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gaaaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaatcacg taagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgttataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 tagcaagagt tttgtactga ccccaaaaag ttagacaaat aatttatccg aaggatttag
     1081 ttctgtattg cacaaggcta agtcctttta gttttacctt aattcgttta ttgttgtagt
     1141 aatcaatata gtctacaata gcttgttcca attgattaag tgatttaaat gttttctcat
     1201 agccataaaa catttccgat ttcagaatcc caaagaagga ctccatcata ccgttgtctt
     1261 ggctgtttcc cttacgtgac atggatgctt gaattccctt accctcaaga aactgatgat
     1321 aagaatcgtg ttgatattgc cagccttggt cactatggag aatcgtattc tcgtagtgct
     1381 tctctgtgaa tgcctgttcc aacattgttt gtacttgttc taagttgggt gaagttgaaa
     1441 gattataggc gataatttcg ctattaaagc catctaaaac tggtgataag taaagctttt
     1501 gagtacttgc tggaatggca aattctgtca catctgtata gcactttccc attgttttag
     1561 agccttcaaa ttggccttga atgagattct ctgccttctt gccaacgtct cctttatgag
     1621 aagaatattt tcgtttctgt cgcattttag cttgtaaatt gagtactttc atcaagcctt
     1681 gaactctttt atgatttacc agataaccac gatttcttag ttctaaataa atccgacgat
     1741 aagcataatt tcccttgtgt tcgataaaaa tggattgaat ttcagcttta agctcttggt
     1801 ccttatctgt tttgtctagc tgtttcaagt gatagtagta ggtcgaacga gctagtttaa
     1861 tggattttag aagaagaact aacgaaaact cagtcattaa ttcttgaaca atttctgtct
     1921 ttcttctttc tctttttcct ccttcaatcg gagttctctt aactttttta ggatggcatt
     1981 ctccgctctc aggtactctc ccttttgttt tctcaacaat agtatacccg tttttcctgt
     2041 attgtgctag ccagttaaga agtatcgtac gacttgggag
//
LOCUS       DAVLYR010000203         1993 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVLYR010000203 DAVLYR010000000
VERSION     DAVLYR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.203"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            151..1926
                     /gene="spxB"
                     /locus_tag="VYO63_001927"
     CDS             151..1926
                     /gene="spxB"
                     /locus_tag="VYO63_001927"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1672771.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
BASE COUNT          637 a          419 c          407 g          530 t
ORIGIN      
        1 tattagaata gagaatagag agttttgagc agatttttag aaaagtcagc ataaatatga
       61 tacagtggaa tagtaaaaat ttggagaacg tttccaattc tatgtaatcg tattctccaa
      121 gtttaaaaaa attgaaggag agttatcatt atgactcaag ggaaaattac tgcatctgca
      181 gcaatgctta acgtattgaa aacatggggc gtagatacaa tctacggtat cccatcagga
      241 acactcagct cattgatgga tgctttggct gaagacaaag atatccgttt cttacaagtt
      301 cgccacgaag agacaggtgc tcttgcagcg gttatgcaag ctaaattcgg cggctcaatc
      361 ggggttgcag ttggttcagg tggtccaggt gcgactcact tgattaacgg tgtttacgat
      421 gcagctatgg ataacactcc attcctagcg atccttggat cacgtccagt taacgaattg
      481 aacatggatg ctttccaaga gcttaatcaa aacccaatgt acaacggtat cgctgtttac
      541 aacaaacgtg tagcttacgc tgagcaattg ccaaaagtaa ttgacgaagc ctgccgtgct
      601 gcagtttcta aaaaaggtcc agctgttgtt gaaattccag taaacttcgg tttccaagaa
      661 atcgatgaaa actcatacta cggttcaggt tcatacgaac gctcattcat cgctcctgct
      721 ttgaacgaag ttgaaatcga caaagctgtt gaaatcttga acaatgctga acgcccagtt
      781 atctatgctg gatttggtgg tgttaaagct ggtgaagtga ttactgaatt gtcacgtaaa
      841 atcaaagcac caatcatcac aactggtaaa aactttgaag ctttcgaatg gaactatgaa
      901 ggtttgacag gttctgctta ccgtgttggt tggaaaccag ccaacgaagt ggtctttgaa
      961 gcagacacag ttcttttcct tggttcaaac ttcccattcg ctgaagttta cgaagcattc
     1021 aagaacactg aaaaatttat ccaagtcgat atcgaccctt acaaacttgg taaacgtcat
     1081 gctcttgacg cttcaatcct tggtgatgct ggtcaagcag ctaaagctat ccttgacaaa
     1141 gtaaacccag ttgaatcaac tccatggtgg cgtgcaaacg ttaagaacaa ccaaaactgg
     1201 cgtgattaca tgaacaaact cgaaggtaaa actgagggtg aattgcaatt gtatcaagtt
     1261 tacaatgcaa tcaacaaaca tgctgatcaa gacgctatct actcaatcga cgtaggtaac
     1321 actactcaaa catctactcg tcaccttcac atgacaccta agaacatgtg gcgtacatct
     1381 ccactctttg cgacaatggg tattgccctt cctggtggta tcgctgctaa gaaagacaat
     1441 ccagatcgcc aagtatggaa catcatgggt gacggagcat tcaacatgtg ctacccagac
     1501 gttatcacaa acgttcaata cgaccttcca gttatcaacc ttgtcttctc aaatgctgag
     1561 tacggcttca tcaagaacaa atacgaagat acaaacaaac acttgtttgg tgtagacttc
     1621 acaaacgctg actacgctaa aattgcggaa gctcaaggag ctgttggatt cacagttgac
     1681 cgtatcgaag acatcgatgc agttgttgca gaagctgtta aattgaacaa agaaggtaaa
     1741 actgttgtca tcgatgctcg catcactcaa caccgtccac ttccagtaga agtacttgaa
     1801 ttggatccaa aacttcactc agaagaagct atcaaagcct tcaaggaaaa atacgaagca
     1861 gaagaactcg taccattccg tctcttcttg gaagaagaag gattgcaatc acgcgcaatt
     1921 aaataattcc tctcgccgaa aatcaaatat gaaacttgta gactttattc tttgattttc
     1981 atagagcaga act
//
LOCUS       DAVLYR010000204         1955 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVLYR010000204 DAVLYR010000000
VERSION     DAVLYR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.204"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            74..763
                     /gene="coaB"
                     /locus_tag="VYO63_001928"
     CDS             74..763
                     /gene="coaB"
                     /locus_tag="VYO63_001928"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1672772.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            775..1326
                     /gene="coaC"
                     /locus_tag="VYO63_001929"
     CDS             775..1326
                     /gene="coaC"
                     /locus_tag="VYO63_001929"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1672773.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1310..1873
                     /locus_tag="VYO63_001930"
     CDS             1310..1873
                     /locus_tag="VYO63_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1672774.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT          601 a          430 c          329 g          595 t
ORIGIN      
        1 gtaaatcttc gtttttacca aaaatttatc actttcattt tacttaccgc ttatttttgt
       61 gtacaatagt gctatgaaaa ttttagttac atcgggcggt accagtgaag ctatcgatag
      121 cgtccgctct atcactaacc attctacagg tcacttgggg aaaattatca cagagacttt
      181 gctttctgca gggcatgaag tttgtttaat tacgacaaaa cgagctctga agccagagcc
      241 tcatcctaac ctaagtattc gagaaattac caataccaag gaccttctaa tagaaatgca
      301 agaacgtgtt caggattatc aggtcttgat ccactcaatg gctgtttctg actacactcc
      361 tgtttatatg acagggcttg aggaagttca ggctagctcc aatctaaaag aatttttaag
      421 caagcagaat catcaggcca agatttcttc aactgatgag gttcaggttt tgttccttaa
      481 aaagacaccc aaaatcatat ccctagtcaa ggaatggaat cctactattc atctgattgg
      541 tttcaaactg ctggttgatg ttaccgaaga tcatctggtt gacattgcac gaaaaagtct
      601 tatcaagaat caagcagatt taatcatcgc gaatgacctg actcaaattt cagcaggtca
      661 gcaccgagct atatttgttg agaaaaatca gcttcaaaca gtccagacta aagaagaaat
      721 tgcagaactc ctccttgaaa aaattcaagc ctatcattct tagaaaggaa aactatggca
      781 aacattctct tggctgtaac gggttcaatc gcctcttata agtcggcaga tttagtcagt
      841 tctctaaaaa aacaaggcca tcaagtcact gtcttaatga ctcaggctgc tacagagttt
      901 atccaacctt tgacactaca ggtactctca cagaatcctg tccacttgga tgtcatgaaa
      961 gaaccctatc ctgatcaggt caatcatatc gaacttggaa aaaaagcaga tttatttatc
     1021 gtggtacctg caactgctaa cactattgca aaactagctc acggatttgc ggacaacatg
     1081 gtaaccagta cagctctagc cctaccaagt catattccca aactaatagc tcctgctatg
     1141 aatacaaaaa tgtatgacca tccagtaact cagaataatc tgaaaacatt agaaacctac
     1201 ggctatcagc tgattgctcc taaggaatcc ctactagctt gtggagacca cggacgagga
     1261 gctttagctg acctcacaat tattttagaa agaataaagg aaactatcga tgaaaaaacg
     1321 ctctaatatt gcacccattg ctatcttttt tgctaccatg ctcgtgatac actttctgag
     1381 ctcacttatc tttaaccttt ttccatttcc aatcaaaccg accattgttc atattcctgt
     1441 cattattgcc agcattattt atggtccacg agttggggtt acacttggat ttttgatggg
     1501 attacttagc ttgacggtta acacgattac gattctaccg acaagctacc tcttctctcc
     1561 cttcgtacca aacggaaaca tctactcagc tatcattgcc attgtcccac gtattttgat
     1621 tggtttaact ccttacttag tctataaact gatgaaaaac aagactggtc tgattttagc
     1681 tggggctctt ggttccttga caaatactat ctttgtcctt ggaggaatct tcttcctatt
     1741 tggaaatgtt tataatggaa atatccaact tcttctggca accgttatct caacaaattc
     1801 aattgctgaa ttggtcattt ctgcaattct aaccctagcc attgttccac gactacaaac
     1861 cttgaaaaaa taaaaacaat ctccgctttc aaacctgaag gtggagattt tgctctatac
     1921 agtttctttt taatgaaatc tttactatag taaaa
//
LOCUS       DAVLYR010000205         1943 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVLYR010000205 DAVLYR010000000
VERSION     DAVLYR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.205"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            254..1195
                     /locus_tag="VYO63_001931"
     CDS             254..1195
                     /locus_tag="VYO63_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1672775.1"
                     /translation="MLTYFVTLKIIYYFHLELAYFDKDYLYPFLKKVFFALLLLHIVS
                     FVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQ
                     NYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRVYNEVLVKANHKLRSDK
                     YTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLL
                     NNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGV
                     GLNNVKELLDKYNNIILETEMEGSTFRQIIRFKRKFE"
     gene            1192..>1943
                     /locus_tag="VYO63_001932"
     CDS             1192..>1943
                     /locus_tag="VYO63_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1672776.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNTPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQI
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHAQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
BASE COUNT          659 a          277 c          357 g          650 t
ORIGIN      
        1 aatagtactt gattttccct tctatcttct atatttagat gggttaggga ttgcaacatt
       61 tttatttcct ttgggcctat attcctattt tcgatggatg aaacagtatg agagggatag
      121 aggactattt ctaagtttac tactatctct tttatatgag agcactcata actttctgtc
      181 cgtaactttc tcttctataa caggagacaa ttttgtttta cagtattatg gcctattctt
      241 tttcgttgtg acggtgttga cctattttgt tacattaaaa atcatttatt atttccattt
      301 ggaactagcc tattttgaca aggactacct ttatcctttc ttgaaaaaag tattttttgc
      361 tttactattg ctacatattg tatctttcgt ttcagatatg gtaagtacga ttaaacattt
      421 gaatagtttt ggaagtattt tgtcatctat tgtctttatc tctctccttt tgaccttctt
      481 tgcaatgaat tctcataaag ttcaaatgga gaaagagatt gctttgaagc agaagaaatt
      541 tgaacagaaa catttacaga attacacaga tgaaattgtc ggtctgtata atgaaatccg
      601 tggttttcga catgattatg ctggaatgct tgtcagcatg cagatggcaa ttgacagtgg
      661 taatttacag gaaattgaca gagtttacaa tgaagttttg gtcaaagcaa atcataaact
      721 acgttcagat aagtacactt actttgattt gaacaacata gaagattcag ctttacgaag
      781 tttggttgct cagtcaattg tctatgctcg aaataatggt gtagagttta cactggaagt
      841 aaaagatacg attaccaagc ttccaattga actattggat ttagtgcgta tcatgagcgt
      901 tttattgaac aatgctgtcg aaggatcggc tgatagctat aaaaagcaga tggaagtagc
      961 agttattaag atggaaactg aaacagttat tgtgattcag aattcatgta aaatgacgat
     1021 gactccttca ggagatctat ttgccttagg attctccact aagggaagaa atcgcggagt
     1081 cggattaaat aatgtgaaag aactactaga taagtacaac aacattattt tagaaacaga
     1141 gatggaaggc agtacattta gacaaatcat tagatttaag aggaaatttg aatgaaagtt
     1201 ttaattttag aagatgttat tgaacatcaa gtgagactag agagaatatt ggatgaaatt
     1261 tcgaaagaat cgaatactcc aatatcatac aagacaacgg gaaaagtccg tgaatttgaa
     1321 gaatacattg aaaatgatga agtaaatcaa ctttatttcc tagatatcga tattcatgga
     1381 attgagaaaa agggatttga agttgctcaa ctcattcgtc attacaatcc ttacgctatt
     1441 atcgtcttta tcaccagtcg atcagagttt gcgactctga cctataaata ccagatatca
     1501 gccctagatt ttgttgataa ggatatcaac gatgagatgt ttaagaagcg aattgagcaa
     1561 aatatcttct acacgaagag tatgttactt gaaaatgaag atgttgtaga ttatttcgac
     1621 tacaattaca agggaaatga tttaaaaatt ccttaccatg atattttgta tattgaaacg
     1681 acaggagtct ctcataaatt gcgcattatt ggtaagaatt ttgcaaaaga gttttatggt
     1741 accatgacag atattcagga aaaggacaaa catgctcagc gattttattc tcctcacaag
     1801 tcatttttgg taaatatagg caatatcaga gaaattgatc gaaaaaactt agaaattgtt
     1861 ttctatgaag accatcgttg tcctatttca agattaaaaa ttagaaaatt aaaagatatt
     1921 ttagagaaaa aatctcaaaa gtg
//
LOCUS       DAVLYR010000206         1876 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVLYR010000206 DAVLYR010000000
VERSION     DAVLYR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.206"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>1876
                     /locus_tag="VYO63_001933"
     CDS             <1..>1876
                     /locus_tag="VYO63_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fn3-like domain-containing protein"
                     /protein_id="HEV1672777.1"
                     /translation="AMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVFSI
                     SGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEIDF
                     KFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTYGY
                     MSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARPMM
                     DATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKEIK
                     GDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPEIH
                     PEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEALN
                     SNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPGTL
                     NKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIANIY
                     PLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFIRG
                     ILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQFEP
                     I"
BASE COUNT          723 a          292 c          364 g          497 t
ORIGIN      
        1 agctatggat aagggtgcac gcgccattat ggttgtaaat actgtaaatt actacaatag
       61 agataattgg acagagcttc cagctatggg atatgaagcg gatgaaggta ctaaaagtca
      121 agtgttttca atttcaggag atgatggtgt aaagctatgg aacatgatta atcctgataa
      181 aaaaactgaa gtcaaaagaa ataataaaga agattttaaa gataaattgg agcaatacta
      241 tccaattgat atggaaagtt ttaattccaa caaaccgaat gtaggtgacg aaaaagagat
      301 tgactttaag tttgcacctg acacagacaa agaactctat aaagaagata tcatcgttcc
      361 agcaggatct acatcttggg ggccaagaat agatttactt ttaaaacccg atgtttcagc
      421 acctggtaaa aatattaaat ccacgcttaa tgttattaat ggcaaatcaa cttatggcta
      481 tatgtcagga actagtatgg cgactccaat cgtggcagct tctactgttt tgattagacc
      541 gaaattaaag gaaatgcttg aaagacctgt attgaaaaat cttaagggag atgacaaaat
      601 agatcttaca agtcttacaa aaattgctct acaaaatact gcgcgaccta tgatggatgc
      661 aacttcttgg aaagaaaaaa gtcaatactt tgcatcacct agacaacagg gagcaggcct
      721 aattaatgtg gccaatgctt tgagaaatga agttgtagca actttcaaaa acacggattc
      781 taaaggtttg gtaaactcat atggttccat ttctcttaaa gaaataaaag gtgataaaaa
      841 atactttaca atcaagcttc acaatacatc aaacagacct ttgactttta aagtttcagc
      901 atcagcgata actacagatt ctttaactga cagattaaaa cttgatgaaa catataaaga
      961 tgaaaaatct ccagatggta agcaaattgt tccagaaatt cacccagaaa aagtcaaagg
     1021 agcaaatatc acatttgagc atgatacttt cactataggc gcaaattcta gctttgattt
     1081 gaatgcggtt ataaatgttg gagaggccaa aaacaaaaat aaatttgtag aatcatttat
     1141 tcattttgag tcagtggaag aaatggaagc tctaaactcc aacgggaaga aaataaactt
     1201 ccaaccttct ttgtcgatgc ctctaatggg atttgctggg aattggaacc acgaaccaat
     1261 ccttgataaa tgggcttggg aagaagggtc aagatcaaaa acactgggag gttatgatga
     1321 tgatggtaaa ccgaaaattc caggaacctt aaataaggga attggtggag aacatggtat
     1381 agataaattt aatccagcag gagttataca aaatagaaaa gataaaaata caacatccct
     1441 ggatcaaaat ccagaattat ttgctttcaa taaccaaggg atcaacgctc catcatcaag
     1501 tggttctaag attgctaaca tttatccttt agattcaaat ggaaatcctc aagatgctca
     1561 acttgaaaga ggattaacac cttctccact tgtattaaga agtgcagaag aaggattgat
     1621 ttcaatagta aatacaaata aagagggaga aaatcaaaga gacttaaaag tcatttcgag
     1681 agaacacttt attagaggaa ttttaaattc taaaagcaat gatgcaaagg gaatcaaatc
     1741 atctaaacta aaagtttggg gtgacttgaa gtgggatgga ctcatctata atcctagagg
     1801 tagagaagaa aatgcaccag aaagtaagga taatcaagat cctgctacta agataagagg
     1861 tcaatttgaa ccgatt
//
LOCUS       DAVLYR010000207         1856 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVLYR010000207 DAVLYR010000000
VERSION     DAVLYR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.207"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            75..>1856
                     /locus_tag="VYO63_001934"
     CDS             75..>1856
                     /locus_tag="VYO63_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1672778.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSIL"
BASE COUNT          726 a          228 c          403 g          499 t
ORIGIN      
        1 cgtattaagt acacaaaata gctgactttt tgatttgaat gcggtaatat agaatcaaaa
       61 ggaggaattt cagtatgaaa aaaaataatg ttgaaataat aaaagctgat tctttagttc
      121 gtcgaagagg cgacaatgta gaaagacact taaagagggt tgctgcttat tgcagagtta
      181 gttccgatag tgaggatcag aaaaacagtt atgattctca agtaagacac tacaaagaat
      241 atatttctca gcgttcagat tgggagctgg cagatattta tgcagatgaa ggtatttcag
      301 gaacacaagt aggaaagaga caagattttc aacgattaat caatgattgt gcaaatggag
      361 agatagatta tattgtcaca aaagcaattg ctagatttgc aaggaatacc ctagatactt
      421 tgaagtatgt cagaatgtta aaggatatgc aaattggagt atattttgag gaagaaaaca
      481 tagataccct aacaatggat ggagaattgc ttctaactat tctaagctcc gttgcacagc
      541 aagaagtaga gaatacttct gcccatgtga aaaaaggatt aaaaatgaag atgcaacgtg
      601 gtgaattggt tggttttcaa gggtgcttag ggtatgatta tgatgtagaa accaagcaga
      661 tttcaattaa taaaaaagaa gccaaaattg ttcgttatat ttttgaaagg tatctagaag
      721 gtattggagg aaaagtaatt gcaagggaac ttgatgaact tggttacaaa tcgccaagag
      781 gtttagaaca ctggaatgat acaacagttc taggaataat aaagaatgaa aaatataaag
      841 gtgatattct aatgggaaaa acttttacag ttgacccaat aagcaagaga cggttgagta
      901 attttggaga agaagataag tactatatca aggataatca tgaaccaata atatctaaag
      961 aagattttga aaaagctcag gagattagat tgcgtcgagc aggaaataag aagacagctg
     1021 cgaatgtaaa tgggaagcgt gaacgctatt caaaaatgta tgcttttagt agcatgttgg
     1081 aatgtggttt ttgtggttca atattatcta gaagaagttg gcactgccgt tcagattatc
     1141 gcaaagttgt gtggcactgt gttacatcga taaagaaagg aaagaaattt tgtaagcata
     1201 gtaaaggatt agaagagatc gccattgagg gggctttcct ggaagcttac agacaagtct
     1261 atcattcaaa tgaaaattta atgacagact tacttgaaac gatagaatct gaattgaatg
     1321 acaatagcct taacaaggaa ctaaaaagga ttacaaacaa acttcgaata ttactaaaaa
     1381 aagaggagaa tcttgtaaat ctaaggctcg aaggaaaagt tagcgattcc atatacaatg
     1441 aaaagtacaa tgaaatttcc tcagaaaaag agtttctagc agaagagaag gtaaatattg
     1501 aaacaacatt aaaatcagaa atcgatgtaa agaaaagact aactgagttt aagcacttgc
     1561 tatcttcgca aaaaatgctt acggagtttg atcgtgcagt ttttgaaagt attgttgaga
     1621 aaattattgt aggtggagtt aatagcaacg gagaaattga tcctgcaatg ttaaccataa
     1681 tattcaaaac aggtgaaatt caaaacaagg atggtaaaca atttaaaagt aaacgtaaaa
     1741 atgctaaact agaaacagat aaattgtgtc ctcaaaacag tgacgaggat aaaaaattgt
     1801 attctcaagg aacagacaac acacgtggag tgtgtagtgt tgctggttcg atctta
//
LOCUS       DAVLYR010000208         1848 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVLYR010000208 DAVLYR010000000
VERSION     DAVLYR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.208"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(173..1804)
                     /locus_tag="VYO63_001935"
     CDS             complement(173..1804)
                     /locus_tag="VYO63_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1672779.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNIIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          562 a          367 c          389 g          530 t
ORIGIN      
        1 tgaaataata acaggacaaa tcgatttcta acaatgtttt agaaatagag gtgtactatt
       61 ctagtttcaa tatactatat atctgcagaa tactctttca gtttcattca gcctagtctt
      121 gcttaggaaa aagtaagaag ccatccaatt atgatggata gcttcttggt tattagattt
      181 gttctgcaaa aaccttttca gcaaggttca tagcgtggtc tgatacacga gtgtagtgtg
      241 agatgatgtc gataaagttg accccagctt gtgttgaaca ttcgcctttg ttgaggcgtt
      301 tgatgtgggt ttttctgaga acacgttcta tcttattgat tgcttcatga cgttctacaa
      361 gactgcgtgc tttttcaata tcattgtttt ccacactatc cagagcatct ttgataaagt
      421 cactagtttg gcggtaaact tcctctaatt ctttcaaggc ggcatcagaa aattcaacat
      481 ttttccgttg aagatagtca gtcagattga gtagagcctc cgtgtggtct ccaatccgtt
      541 ccaaatcacg ggaggaatca aggatattgg taagcacttc actttctttt tggctgagag
      601 cttcgcttga aagggcaatg agataacgtg ttaattgctc atcgatggtg ttaattgctt
      661 cttcggtttt atgccctttt tcagcaactt tttcatccaa gtcaatgatg tacttatagg
      721 aaaggtcaaa ggctttagca gcgtagtttc ctaagtgcaa gagctctttc ttagcatttc
      781 ctagagcgat agatggggcc tgtttgatga aatgttcatc aagatataag ggttcgtatt
      841 tgacaacctc gtcctctcca ggaataatct tggttacaaa gtaagccaga gctccgataa
      901 atggaaattg gacaatggtg ttggtaatat taaaggttcc gtgagcaaag gcgatggtca
      961 tttccggtgc tagatttagc gtagcttcaa accaatgaat caggacagta aaaggaacta
     1021 gaaaaataac gcagacgact gttccgataa tgttgaaggc aacatgagct cctgctaccc
     1081 gtttagctgc aatattagcc cctaaagagg caatgatggc tgtaatggtt gtcccgatat
     1141 tgtcaccaaa tagaactggc aaagctccct gtaggtcaat tagattgccg gcgtagaggt
     1201 tttgtaaaat cccaatggta gccgaagaag cttgaattag caaggtcaag ccagtaccga
     1261 caaagacacc caaaacagga ttcttactta gctcaatcat atagtcctta aagacctgta
     1321 aatccttgag tggagccatt gcgccgctca tgagattgag ggcaaaaaag ataccaccga
     1381 taccaaagag gatgcgtccg atattattga ctgtccgatt tttcgtaaaa aagagacaga
     1441 cggcaccgat aaagagcata ggtagggcat agttacctaa tttaaaaccg atgagaaagg
     1501 atgtgacagt tgtcccaata ttagcaccca tgacaatccc gatagcctga cgtaaggtta
     1561 agagaccggc actgaccagg ccgactgtaa taactgttac accagaacta gactgaatta
     1621 gagcagtcat cccaatacca accagaactc caaagaaagg attactagta tatttgtcaa
     1681 tgtaaaaacg aaggcgatct ccagcagctt gttgcaaacc gtctcccatg gtcttgatgc
     1741 tatataagaa tagccccaga ccacctaaaa agtgaaataa aatttcctgc caattaatgg
     1801 acatttcttt ttcctccgaa aaataatcgc ggaatatctc ctattcta
//
LOCUS       DAVLYR010000209         1847 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVLYR010000209 DAVLYR010000000
VERSION     DAVLYR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.209"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1582
                     /locus_tag="VYO63_001936"
     CDS             <1..1582
                     /locus_tag="VYO63_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEV1672780.1"
                     /translation="TVLLDEKNIELLLSKAPYLVSMIVEDFSKLSVDDFSLDNNDLKI
                     NLPSPMNEPVVGVIDTLFDKRVYFNEWVEYHDFVSPDISKDSQDYKHGTAVTSLIVDG
                     ANLNPNLDDGCGNFRVRHFGVSLQSGFNSFTIIKQIKEIVSQNADIKVWNLSLGSNDE
                     IRENFISAEGALLDEIQFENDVIFIIAGTNASVINGKRKRIGAPADSLNSIIVNSVDF
                     NNQSVSYSREGIVLSFFVKPDVSYYGGGNGDFINVCEPLGLGRVAGTSFAAPFIARKM
                     AYLIHIMGLSREEAKALLIDAAIPWNDKKTFTDLSLIGNGIVPIKMDDILSTPDDEIK
                     FIVSDISRAYDTYNYDFPVPISSESYPYVAKATMCYFPNCSRKQGVDYTNTEMQLTFG
                     RLKSDGIKSINKDNQHAEDTPGYVRENAARNIFRKWDNVKHIGESFTSRKRAKAILNP
                     SNPQWGMSIKTIERLKSGDGQGVRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQ
                     VEAQVDLFNSLNEEIEFE"
     gene            1633..>1847
                     /locus_tag="VYO63_001937"
     CDS             1633..>1847
                     /locus_tag="VYO63_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1672781.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYRDFYEFQNKVYISRTENEEKYSN
                     FIYESLFSDKVNDYENWSDFELSIGKLT"
BASE COUNT          624 a          239 c          377 g          607 t
ORIGIN      
        1 cacggtcttg cttgatgaaa aaaatattga attgctacta tcaaaagcgc cctatcttgt
       61 ctccatgatt gtggaagatt tttctaagtt atctgtagat gatttttctt tagataataa
      121 tgatttaaaa ataaatcttc catctccaat gaacgaacca gttgttggcg ttattgatac
      181 cttatttgac aaaagagttt actttaatga gtgggtagaa tatcatgatt ttgtctcacc
      241 tgatattagc aaagatagcc aagattacaa gcatgggaca gctgtgactt ctttaatcgt
      301 tgatggagcc aatttgaacc ctaatctgga tgatggttgt ggaaatttta gagtgaggca
      361 ttttggtgtg tcactacaaa gtggatttaa ttcatttaca atcataaaac agataaaaga
      421 gattgtttct cagaatgctg atattaaggt ttggaatctt tctttagggt ccaatgatga
      481 aataagagaa aattttatct ctgcagaggg agcattatta gacgaaattc aatttgagaa
      541 tgatgtaatt tttatcatag ctgggacaaa tgcttcagta ataaatggga aacgcaaaag
      601 aataggtgct cctgctgatt cacttaattc tataatagta aattctgttg attttaataa
      661 tcaatcagtg tcatattcac gagagggtat tgttttatca ttctttgtta aaccagatgt
      721 gtcatattat ggaggaggaa atggtgactt tataaatgtt tgtgaacctc taggattagg
      781 tcgtgtagct ggaacaagtt ttgcggcacc ttttattgca aggaaaatgg cctatctcat
      841 tcatattatg ggacttagcc gagaagaagc gaaggctttg ctaattgatg cagcaatacc
      901 ctggaatgat aagaaaacat tcactgattt atctttgata ggaaatggta ttgttccaat
      961 aaaaatggat gatattttat ctacacctga tgatgaaatt aaatttattg tgtcagatat
     1021 ctctagggct tatgatacct ataattatga ttttccagta cctatttcta gtgagagtta
     1081 tccatatgta gctaaagcaa caatgtgtta tttccctaac tgctcaagaa aacaaggggt
     1141 tgattataca aatactgaga tgcagcttac ttttggtagg ttaaagtcag acggtatcaa
     1201 atcgataaat aaagacaacc agcatgccga agatacccca ggatatgtga gagaaaacgc
     1261 tgctagaaat atatttagga aatgggataa tgtcaagcat attggagagt cgtttacttc
     1321 tcgaaagaga gctaaagcaa ttttaaatcc atctaatcct cagtggggga tgagtattaa
     1381 aacaatcgaa cgtttgaaaa gcggtgatgg acaaggagta cgttttggtg tagtagttac
     1441 attaaaagaa ttaaatgggg ttaatcgtat tgaagatttt attcagcaag ctgaattacg
     1501 cggttggcta gttaatagat tgcaagtaga agctcaagta gacctcttta atagtttaaa
     1561 tgaagaaatt gaatttgaat agtggatatt ttatccttat tttagatctt aggaacacaa
     1621 gagggcaaag atatgaatat tacttatata gttggaaatg gtttggattt gcaatatggt
     1681 ttgaaaacaa gatataggga tttctacgaa ttccaaaata aggtttatat aagtaggaca
     1741 gagaacgagg aaaaatattc aaatttcatt tatgagtctc tattttctga taaagtaaat
     1801 gattatgaaa attggtcaga ttttgaattg tcaattggta aattgac
//
LOCUS       DAVLYR010000210         1837 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVLYR010000210 DAVLYR010000000
VERSION     DAVLYR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.210"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            25..912
                     /locus_tag="VYO63_001938"
     CDS             25..912
                     /locus_tag="VYO63_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1672782.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(955..1572)
                     /locus_tag="VYO63_001939"
     CDS             complement(955..1572)
                     /locus_tag="VYO63_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1672783.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
BASE COUNT          561 a          362 c          428 g          486 t
ORIGIN      
        1 gaaaataaaa acaagaggtt tatcatgaca aaattatatg gaagcttgga agcgggcggt
       61 acaaagtttg tctgtgctgt cggtgatgaa aactttaacg ttgtagaaaa aacacaattt
      121 ccaacaacaa ctccaatcga aacaatcgat aaaaccattg agttcttctc aaaattcgat
      181 aaccttgctg gtcttgcagt tggttcattt gggccgattg atattgacaa aaactcaaaa
      241 acttatggct ttatcacgac gactccaaaa ccaaactggg caaatgtgga cttgcttggt
      301 gcccttcgtc gcgccctaaa tgtgccaatg tacttcacaa cagacgtaaa cagctctgct
      361 tacggtgaag tggttgcccg taacaatgct ggtggtcgta tcgaaaactt ggtttactac
      421 acaatcggta caggtatcgg tgcaggtgtc atccaacgtg gtgagtttat cggtggtgtg
      481 ggtcaccctg aaatgggtca ttattatgtt gctagacacc caatggatat tgaaaaagag
      541 tttaagggtg tttgtccttt ccataaggga tgtctagaag gttatgcagc tggtccaagt
      601 ttggaagctc gtacaggtgt acgtggggaa actattgaac tcaacaaccc tgtttgggat
      661 gttcaagcct actatatcgc tcaagctgcg gttaatgcga cagtgacttt ccgcccagac
      721 gtgattgtct ttggtggagg ggtcatggct caacaacata tgctggaccg tgtccgtgag
      781 aaatttacat ctcttcttaa tggttaccta ccagtaccag atgtgcgtga ctatatcgtg
      841 acgccagcag tcgcaggaaa tggttctgcc acacttggga actttgttct tgcaaaagaa
      901 gtttcaaaat aaagcacaaa atccgcttgg gaaaccaaac ggatttttta cgtgtcaaag
      961 catttgccag aaaaagtcaa taatataggt cagaccgtag gtataaacca cgagggtaaa
     1021 gggtttggtc agaatgatga tggtcatgta gcgcttgaaa ctcatcttgg tcagggcagc
     1081 cagcatacag agaaagtcag ctgggctaat gggccaaatc atcataaaaa taaagaagcg
     1141 gtcaaaacga ttgcccttat ctagccagcc gatgtacttg tcgtaggtgc gcttgctgac
     1201 gacagactgg acaaaggcag ctccgtatag gcgcactaga taaaagataa tggcacagcc
     1261 aatcacgatg ccgatatagt tgtagatagt cccgatgatg tgcccgtaga taaagacccc
     1321 agccaccgag gtcaaggccc ctggaatgat agggacgaca gtctgtaaaa tctgtaaaaa
     1381 gataaagaga ggtggacccc agatgcctgc ctgctggata aaggcagaga gggtttcctt
     1441 ggattgtaaa atcccagcct gataagccca aatacagaaa atcaaactcc caaccccacc
     1501 gacaatcgat gagatattga taactcgctg cagaagggga gaaacagctt tcatttgttt
     1561 atcctgtgac atgtaaactc ctcatagata gtatatttct actataccat attttggagg
     1621 agaaaggggg aatgagtctt ttttgataag gaatagaaag taaatagccc ttcgagactt
     1681 agaataggtc tgtcaactaa aggttatatg cattctaggt ttcgaataat cgcaattcca
     1741 taaatttaaa agtattctaa gtttcgaata gcttatctgg gataattttg aggcattcgc
     1801 gctttcgaat agtcattttc cctaaagttt atatgta
//
LOCUS       DAVLYR010000211         1837 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVLYR010000211 DAVLYR010000000
VERSION     DAVLYR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.211"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..689)
                     /locus_tag="VYO63_001940"
     CDS             complement(<1..689)
                     /locus_tag="VYO63_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1672784.1"
                     /translation="MVLSTLAILVACGKTDKEADAPTTFSYVYAVDPASLGYSITTRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPIVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKEL"
     gene            complement(745..1767)
                     /locus_tag="VYO63_001941"
     CDS             complement(745..1767)
                     /locus_tag="VYO63_001941"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1672785.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
BASE COUNT          531 a          397 c          312 g          597 t
ORIGIN      
        1 tctagttctt tttggtattt gctattggat tccaattttt ctttttgcca ttttttaaaa
       61 acctcgttat attcttttgt tgtaacaata tctttttgca atttcattcc tttaaagata
      121 tatggttccc ccttaatacc aacttgtgag tatggttttg agaatggtac tacgttactt
      181 acaattggag aaccaccaga tgaagctgtt ggcatcaata atgaactatc tgtcgaccaa
      241 gcttgagctt tggcatattt ttcatatctc ttctctaggt cagtggtctc agaaacagca
      301 tcttctaaca atttcttata tttatccaaa ccaggtttag ctacaacatc cttatctttt
      361 cctttcgtaa taccaaggtg tttcatggca gaaccagatt ttggatccat aatattcaag
      421 taagacgctg gatcttgata gcttggagcc catcctgtac tattcaaatc atagtctttt
      481 tgagaaggag caacattgcc gtatttatca ttttccatca aaccatcaat aacatttcca
      541 ataacgtctg tcgtcgatgt tcgagtcgtt atactgtagc ccaatgatgc tggatctact
      601 gcatagacat aagaaaatgt tgtcggtgca tctgcttctt tatcagtttt tccacaagcc
      661 actaaaatag ctaacgtgct caggaccact cctgctgtta agagccactt tttctatttc
      721 ataaaaaatc tcctttggtt tattttaatc tacttttaca atccaacctt ctggcgcttc
      781 aatatcgcca aactgaatac ccgtcaattc attatataat ttacgcgtca caggacctac
      841 ttctgtttca ctatagaata catggaaatc atcaccatgt tgaatacctc caattggaga
      901 aataaccgct gctgtaccac aggcacctgc ctctacaaaa cggtcaagat tatcaattgg
      961 aacatcaccc tcaataggag ttaatcccaa gcgatgttct gccaaataaa gcaaggaata
     1021 cttggtaata gatggcaaga tagatggact caatggtgtt acaaattcat tatcagctgt
     1081 aattccaaag aaattagctg atccgacttc ttcaatcttt gtatgagttg atgggtccag
     1141 atagataaca tctgagaaat ggcgtgactt ggccattttt cctggtaaga gacttgcagc
     1201 atagtttcca ccaaccttag ccgcacctgt accatttggt gctgcacggt cgtactcatc
     1261 ctgaatcaag aagttggttg ggaccaaacc acctttaaag taatttccaa ctggcatagc
     1321 aaagatggtg aaaatgtact cttctgccgg ttttaccccg ataatatctc cgacaccaat
     1381 caaaagaggg cgaagatata aggttccacc tattccgtat ggtggtacgt attcttcatt
     1441 cgcacggaca actgctttac aagcttctac aaacatgtct gtcggaactt gtggcatcaa
     1501 gagacggtca catgtacgtt gcaggcgttt agcattttca tcaggacgga acagttgaac
     1561 actgccatcc ttagtacgat aagctttcaa accttcaaat gcttgttgtc catagtgaag
     1621 acttggagaa gactctgaaa tatgcaaagt tgcatcctct gtaagctctc cttgatccca
     1681 ttgtccattt ttgaaatgag caagatagcg ataaggtaat ttcatatagg aaaaaccgag
     1741 gttttcccaa tcaatcgtta ctgtcatatt ccactcctta ttctaaaaac ctatttctta
     1801 tattctacac tatttttcta aaatagcaag tatattt
//
LOCUS       DAVLYR010000212         1723 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVLYR010000212 DAVLYR010000000
VERSION     DAVLYR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.212"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            269..1036
                     /locus_tag="VYO63_001942"
     CDS             269..1036
                     /locus_tag="VYO63_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1672786.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            1038..1670
                     /locus_tag="VYO63_001943"
     CDS             1038..1670
                     /locus_tag="VYO63_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672787.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
BASE COUNT          541 a          298 c          350 g          534 t
ORIGIN      
        1 ttaatttaat tgagttggaa gagagaattc aaactactat tggaattaaa caaataaaca
       61 cacacaatat tattactatt gcccgagaag ggtactctca aaattattta cctaacactt
      121 cagaaaatac atataattca ttacaagtca gtttagttgg agtattacta ctttttataa
      181 gtatggtaaa tattttatgg gctaaaaaaa gtaaatgaaa ataaaatttg gagccctctg
      241 aaaaagtaag tcctacagtt caactaaaat gagtcaaaag atgaatcacc ttgatgtagg
      301 ggagtttgtc ttattgctgc ctgaacacct ccgttcagag gaagaacatt ataaatctgt
      361 ttttgaagac gacttaacca gtcgcatatc tagtcaagat gaacgacagc aaatgactgc
      421 tacggtaggt tatttagaat caggtcagga tcgttttgtg tataatacga cccctatttc
      481 ttaccagcag tttttgaaag atccaatcat cattgttata acaccccaat caactggtcc
      541 acagtccatt ttgttttgga tagacgcagt acagaactac gttctcttta atcaattgtc
      601 tgatgcccag gagcttatcc agagacaagg cattgaaaat tgggtctcag aaatgcaaac
      661 aggttaccac aactacatca cattattgga taatatccag agggaacgtt gggtaatgct
      721 agcaggagct gtgcttggga ttgcaacttc aatcttgttg tttaacacta tgaataggct
      781 ctactttgaa gaatttagac gtgccatttt tatcaaacgc attgcaggtc tcaggttctt
      841 agaaatccat cgcacttatc tctttgctca actgggtgtg tttttactgg gatttgttgc
      901 gagtgtattt cttcaggtag agataggagt tgctttctta gtcttgttac tctttactgg
      961 tctatctctt ttacagttac atgtccaaat gcagaaagaa aacaagatgt ccatgcttgt
     1021 tttgaaggga ggttaatatg attgaactta aacaggtgag taaatctttt ggagaacgag
     1081 agttattttc gaatctttca atgacatttg aggctggaaa agtctatgcc ttaattggtt
     1141 caagtggtag cggaaaaaca accttgatga acatgattgg gaaattagaa ccttatgatg
     1201 ggacgatttt ttaccgaggt aaagacttgg ccaattataa atcaagtgat tttttccgtc
     1261 acgaattggg ctacctcttc cagaactttg gcttaattga aaaccaaagt attgaagaaa
     1321 accttaagct aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg ttgaggcaga
     1381 agcaggcttt agaacaggtc ggcctggttt atcttgacct agataagcgc atctttgagt
     1441 tatcgggcgg agaatcgcaa cgggttgcct tggcaaaaat tatcttaaag aatccaccct
     1501 ttattctggc agatgagcca acagcttcaa tagacccagc aacctctcag ttgattatgg
     1561 agattttgct atctcttcga gatgataata ggctaatcat tatcgcaaca cataatccgg
     1621 caatttggga gatggctgat gaagtgttca cgatggatca tctgaaataa aaatccttgt
     1681 ttttaattgc acgatgagtt actgaaatat tatcatgaat caa
//
LOCUS       DAVLYR010000213         1706 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVLYR010000213 DAVLYR010000000
VERSION     DAVLYR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.213"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(549..1529)
                     /locus_tag="VYO63_001944"
     CDS             complement(549..1529)
                     /locus_tag="VYO63_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1672788.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKKGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT          533 a          362 c          324 g          487 t
ORIGIN      
        1 aaaaagaaaa cagaaactca tctgttttta tactcaatga aaatcaaaga gcaaactaag
       61 aagctagccg catgatgctc aaaacactgt tttgaggttg tagataagac tgacaaagtc
      121 agctcaaaac accgttttga ggtggcagat agaactgacg aagtcagtaa catatatacg
      181 gcaaggcgaa gctgacgtgg tttgaagaga tttttgaaaa gtattaacac ctacagacaa
      241 gcctaacgat agcaagaact accctactcg ataggtatcg gcttttgctt tctgaaaaaa
      301 attattttaa gcaatttgac aaatctagca acaaaaaatt ctataaatat aatagattga
      361 aactagaata gtacacatct acttctaaaa cattgttaga aatcgatttg actctcctga
      421 tcgatttgtc ctgttcttgt ttcattttac tatatttcca tgataaaacg catagtatca
      481 agttttctta atcccctgat actatgcgtg tttgtaattt ttaagatttt gtgcttagag
      541 tcgactcctt attttagata tttaaaagga atctcacttc cacagagcca gttgtagact
      601 tggtcattga caaatacatt catggcttcg tgagcatact caggcatgat gcgataggtt
      661 ttatcgcagg tcagacgatt ataaatcgca aactgggtaa tgggatagca aacatcgtcg
      721 tccaagcccg taatcatctt aacctcacct tggatacgat gggcaagatt tttgacatcg
      781 atataggcaa gggtcgccat gatttcctcc tctgtttcat ggaaggggtc gtgaaacttg
      841 aaataacgga aaagttcgtc gtaagcctcg ctagtattac caatctcaat cacccgtctg
      901 aagtctgaca agaagggata aatggcaact gttttctgaa ttcgaggatt gagcgctgct
      961 gcaactagag ctagagcccc tccttgtgag gcaccatagc tagaaagacg cttctcatca
     1021 acctgagaca gactagcaac aatttcgacc aactggtaaa tatccagata aacatcctta
     1081 taaaagaggt ggtcccgacc ttccacagca ccacggataa tatgcccctt cacggtattt
     1141 cctaaaggag aacgcaagcc gtcttgtgag taacctgact ggccccgcac atccatggaa
     1201 acaacaccgt aaccagctac ggtgaagccc agcatgtcgg cccagtccca gccacgtccc
     1261 atataaccat gaaaatggaa gattagtgga accttctcct cactctttgg aagaacaatg
     1321 cgtgcataga cctttccttt cttgcttcct tcaaatgtta actcatagca cttgacttga
     1381 ggaatgtgga aatctctttc ctccaagtgg taggatggaa gcgtggaaac atttttcact
     1441 tccccatccc agaaatcatc aaagtcttcc ggaacctcat cccttcctct ataggtctta
     1501 atttcttcta gcaaagctgg atttttcata gcatgctcct tttattttgt aatcgttacc
     1561 acaactgtag catatctatg taatcaatgc aagaaagaag ggtaaataga aagtgaattt
     1621 agattcttta cttttaggat gatgggctga aagtagtttt agaaacgaaa aaagagcatt
     1681 tcgctctctc ttctgtttat actagt
//
LOCUS       DAVLYR010000214         1680 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVLYR010000214 DAVLYR010000000
VERSION     DAVLYR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.214"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            100..300
                     /locus_tag="VYO63_001945"
     CDS             100..300
                     /locus_tag="VYO63_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006269016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672789.1"
                     /translation="MYNKNQKVEQFIKKILLTRSYRNNDRLLQFQGEDGINSYFDFGV
                     GNLGIYWTLLGYTFPFELSKGD"
     gene            302..1540
                     /locus_tag="VYO63_001946"
     CDS             302..1540
                     /locus_tag="VYO63_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672790.1"
                     /translation="MHIFLKNRAFRQLTVNEWISSFGDTIFYLAFINYVSSYAFAPLA
                     IFLISLSETIPQVLQLFTGVIADFQKNRISKYISILFIKVLLYSGVTLLLTSTDFSLF
                     SVFFICSMNLISDTIGFLAGYMLTPIYIRLINDDMTEAMGFRQSTSSIVRLIGNLSGG
                     VFLGLFSISTLAFVNVLTFLFAFLGSLLIRNRLKKEEEKIEVPPYVGMSSFFQHLKES
                     MKLLMTMEDVMVLLWILSISQAVLMMVEPVSAILLIHHPFMGLSTGQSLAILIMISLL
                     HVILGGLLSGFLSKKISIRLNIYWSLLMESLIVIDFLRGSFLLILLGSAGDAFSAGVL
                     SPRLQAMIFGIIPEELMGSVQSSINVINLLIPAVLSLALVFLATSAGLEVVAFALIIL
                     LLIAAYLVHQMKNLPNQEEV"
BASE COUNT          474 a          281 c          307 g          618 t
ORIGIN      
        1 tctgagataa tctataaatc acaacttgaa atggtgcaac aatatgaagc aatgggaact
       61 agtttttgcc atggtttaag tagtttattg caaacaacaa tatacaataa aaatcaaaag
      121 gttgaacagt tcataaaaaa aatcttattg actcgttctt atcgtaataa tgatagatta
      181 ttacaatttc aaggggaaga tggaataaac agttattttg actttggtgt gggaaatctg
      241 ggtatttatt ggactttact cggttataca ttcccatttg aattaagtaa aggagattaa
      301 tatgcatatt tttcttaaaa atcgagcttt tcgtcagcta acagtaaatg agtggatttc
      361 aagttttgga gatactattt tttacttagc ttttatcaat tatgtctctt cttatgcctt
      421 tgctccctta gctatatttt tgattagcct atcagaaacc attccacagg tcttgcaact
      481 ttttactgga gttatagctg attttcaaaa aaatcgaatc agcaagtata tttctatcct
      541 ctttatcaag gtcctccttt attctggagt gactttgctc ctgacaagta ccgacttctc
      601 tctcttttct gttttcttta tttgcagcat gaacttgatt tcagatacga ttggttttct
      661 tgcaggatac atgctgacac cgatttacat acgactaata aacgatgata tgacggaggc
      721 tatgggtttt aggcagtcca cgagttccat tgtacgtttg ataggtaatc tcagcggagg
      781 agtattttta ggactgttta gcatttctac tctagctttt gtaaatgttt taaccttttt
      841 atttgccttt ctaggaagtc tacttatccg aaaccgcttg aaaaaagagg aagaaaaaat
      901 cgaagttcct ccttatgtag gaatgagtag ctttttccag catttgaaag agtcaatgaa
      961 attattgatg actatggaag atgtcatggt cttgctttgg attttgtcca ttagtcaagc
     1021 tgttttgatg atggtagagc ctgtcagtgc gattctactg attcaccatc cttttatggg
     1081 tttgtcaaca gggcaaagtc tggcaatttt aataatgata agccttcttc atgttatttt
     1141 aggtggactt ctgagtggat ttctatctaa aaaaatttct attcgcctga atatttattg
     1201 gagtctattg atggagtctc tcattgttat agattttcta cgtggaagtt ttttacttat
     1261 tttactagga agtgcgggtg atgctttctc ggcaggagta ttgagtcctc gcttgcaagc
     1321 catgattttt ggaatcattc ctgaggagtt aatgggatca gttcaatcat ccatcaatgt
     1381 tatcaactta cttatcccag ctgtcctatc ccttgctctc gtatttcttg caacaagtgc
     1441 tggtctggaa gttgttgctt ttgctcttat aattttacta cttatagcag cctatctagt
     1501 tcaccagatg aaaaacttac caaaccaaga agaagtttag ctagaatgtt atggataagt
     1561 cagttttcac ttgatagttt ctatggaatc gtaatctcct tgtaattgat tgtcgatatt
     1621 acatacaact atcttgcaac tacaaatgtt tccaatccct atcaagatgc aatttatgta
//
LOCUS       DAVLYR010000215         1653 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVLYR010000215 DAVLYR010000000
VERSION     DAVLYR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.215"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..110
                     /locus_tag="VYO63_001947"
     CDS             <1..110
                     /locus_tag="VYO63_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1672791.1"
                     /translation="LYDWISTNRYELPKEFSIRMAHKYHESVTEVFGDE"
     gene            160..1410
                     /gene="ilvA"
                     /locus_tag="VYO63_001948"
     CDS             160..1410
                     /gene="ilvA"
                     /locus_tag="VYO63_001948"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1672792.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT          487 a          275 c          369 g          522 t
ORIGIN      
        1 ggttatatga ttggatttcc acaaatcgtt atgaacttcc taaagagttt tcgattcgta
       61 tggctcataa ataccatgaa agtgttactg aagttttcgg agatgaataa ctaaaaaaca
      121 gtcattagtg actgtttttt atagaaaaag aggttttata tgttaagttc aaaagatata
      181 atcaaggctc acaaggtctt gaacggtgtg gttgtgaata ctccactgga ttacgatcat
      241 tatttatcgg agaagtatgg tgctaagatt tatttgaaaa aagaaaatgc ccagcgtgtt
      301 cgctccttta aaattcgtgg tgcctattat gccatttccc agctcagcaa ggaagaacgt
      361 gaacgtgggg tagtctgcgc ttctgcggga aatcatgcgc agggagtagc ctatacttgt
      421 aatgaaatga aaattcctgc tactatcttt atgcccatta ctacgccaca acaaaagatt
      481 ggtcaggttc gcttttttgg tggggatttt gtaactatta aactagttgg agataccttt
      541 gatgcctcag ccaaagcagc tcaagaattt acagtctctg aaaatcgtac ctttattgat
      601 ccttttgatg atgctcatgt tcaagcaggt caaggaacag ttgcttatga gattttagaa
      661 gaagctcgaa aagaatcgat tgattttgat gctgtcttgg ttcctgttgg tggtggcggt
      721 ctcattgccg gggtttctac ctatatcaag gaaacaagtc cagagattga ggttatcgga
      781 gtagaggcga atggagcgcg ttccatgaaa gctgcctttg aggctggagg tccagtcaaa
      841 ctcaaggaaa ttgataaatt tgcggatggg attgctgtgc aaaaggtagg tcagttgacc
      901 tatgaagcaa ctcgtcaaca tattcaaact ttggtaggtg tcgatgaggg attgatttct
      961 gaaaccttga ttgaccttta ctctaagcaa gggatagtcg cagaacctgc tggagcagct
     1021 agtatcgcct ctttagaggt tttagctgaa tatattaagg ggaaaaccat ttgttgtatc
     1081 atttctggag gaaataatga tatcaaccgt atgccagaaa tggaagagcg tgccttgatt
     1141 tatgatggta tcaaacatta ctttgtggtc aatttcccac aacgtccagg agctttgcgt
     1201 gagtttgtaa atgatatcct ggggccaaat gatgatatca cacgttttga gtatatcaaa
     1261 cgagctagca agggaacagg cccagtatta attgggatcg ctttagcaga taagcatgat
     1321 tatgcaggtt tgattcgtag aatggaaggt tttgatccag cttatattaa cttaaatggt
     1381 aatgaaacgc tttataatat gcttgtctga ggactaataa aaaaatatca tattatttgt
     1441 acaactgatg tatatgtgct ataatgaaga tgaagaaagg ggacgattcc tatggtttta
     1501 gaaaacttat cgtgcaattc ttacttctgt tcattatagc acttatgatg tttggctcag
     1561 atttccatcc ttcattttga tttcctatct attgacaagc atagtcacac tgtctttaat
     1621 actcttcgaa aatctcttca aaccacgtta gct
//
LOCUS       DAVLYR010000216         1618 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVLYR010000216 DAVLYR010000000
VERSION     DAVLYR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.216"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            81..>1618
                     /locus_tag="VYO63_001949"
     CDS             81..>1618
                     /locus_tag="VYO63_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1672793.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARVKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIA"
BASE COUNT          628 a          230 c          328 g          432 t
ORIGIN      
        1 gtaaaacttt ttataacaaa ggctagcaaa gttaaagttt attatctatt ggaagttaaa
       61 taaatatgta aggaattaaa atgaaaaaaa gtacagtatt gtcattaacc acagctgcag
      121 ttattttagc agcctatgcc cctaatgagg tagtcttagc agacacatct agctctgaag
      181 atgctttaaa catctctgat aaagaaaaag tagtattaga taaggaaaca gaaaataaag
      241 agaaacatga agatattcat aatgctatag aaacttcaca ggattttaaa gagaagaaaa
      301 cagcagtcat tgagggaaaa gaagttgtta gtaaaaatcc tgtgacagac aataacacta
      361 gcaatgaaga agcaagagtc aaagaagatt ccaatcaatc ccaaggagat catacggact
      421 catttgtgaa taaaaacaca gaaaatccca aaaaagaaga taaagttgtc tatattgctg
      481 aatttaaaga taaagaatct ggagaaaaag caatcaagca actatccaat cttaagaata
      541 caaaagtttt atatacttat gatagaattt ttaacggtag tgccatagaa acaactccag
      601 ataacttgga caaaattaaa caaatagaag gtatttcatc ggttgaaagg gcacaaaaag
      661 tccaacccat gatgaatcat gccagaaagg aaattggagt tgaggaagct attgattacc
      721 taaagtctat caatgctccg tttgggaaaa attttgatgg tagaggtatg gtcatttcaa
      781 atatcgatac tggaacagat tatagacata aggctatgag aatcgatgat gatgccaaag
      841 cctcaatgag atttaaaaaa gaagacttaa aaggaactga taaaaattat tggttgagtg
      901 ataaaatccc tcatgcgttc aattattata atggtggcaa aatcactgta gaaaaatatg
      961 atgatggaag ggattatttt gacccacatg ggatgcatat tgcagggatt cttgctggaa
     1021 atgatactga acaagatatc aaaaacttta acggcataga tggaattgca cctaatgcac
     1081 aaattttctc ttacaaaatg tattctgacg caggatctgg gtttgcgggt gatgaaacaa
     1141 tgtttcatgc tattgaagat tctatcaaac acaacgttga tgttgtttcg gtatcatctg
     1201 gttttacagg aacaggtctt gtaggtgaga aatattggca agctattcgg gcattaagaa
     1261 aagcaggcat tccaatggtt gtcgctacgg gtaactatgc gacttctgct tcaagttctt
     1321 catgggattt agtagcaaat aatcatctga aaatgaccga cactggaaat gtaacacgaa
     1381 ctgcagcaca tgaagatgcg atagcggtcg cttctgctaa aaatcaaaca gttgagtttg
     1441 ataaagttaa cataggtgga gaaagtttta aatacagaaa tataggggcc tttttcgata
     1501 agaataaaat cacaacaaat gaagatggaa caaaagctcc tagtaaatta aaatttgtat
     1561 atataggcaa ggggcaagac caagatttga taggtttgga tcttaggggc aaaattgc
//
LOCUS       DAVLYR010000217         1593 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVLYR010000217 DAVLYR010000000
VERSION     DAVLYR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.217"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..717
                     /locus_tag="VYO63_001950"
     CDS             <1..717
                     /locus_tag="VYO63_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1672794.1"
                     /translation="NYIINYGTLIGAVRHEGFIPWDDDIDLSMPREDYQRFINIFQKE
                     KSKYKLLSLETDKNYFNNFIKITDSTTKIIDTRNTKTYESGIFIDIFPIDRFDDPKVI
                     DTCYKLESFKLLSFSKHKNIVYKDSLLKDWIRTAFWLLLRPVSPRYFANKIEKEIQKY
                     SRENGQYMAFIPSKFKEKEVFPSGTFDKTIDLPFENLSLPAPEKFDTILTQFYGDYMT
                     LPPEEKRFYSHEFHAYKLED"
     gene            719..1528
                     /locus_tag="VYO63_001951"
     CDS             719..1528
                     /locus_tag="VYO63_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1672795.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT          535 a          286 c          284 g          488 t
ORIGIN      
        1 aactatatta ttaactacgg tactctgatt ggggcggttc gacatgaggg ctttatccct
       61 tgggacgacg atattgattt atccatgcct agagaagact accaacgatt tattaacatt
      121 tttcaaaagg aaaaaagcaa gtataagctc ctatccttag aaactgataa gaactacttt
      181 aacaacttta tcaagataac cgacagtacg actaaaatta ttgatactcg aaatacaaaa
      241 acctatgagt ctggtatctt tatcgatatt ttccctatag atcgctttga tgatcctaag
      301 gtcattgata cttgttataa actggaaagc ttcaaactgc tgtctttcag taaacataaa
      361 aatattgtct ataaggatag ccttttaaaa gattggatac gaacagcctt ttggttgctc
      421 cttcgaccgg tttctcctcg ttattttgca aataaaatcg agaaagaaat tcaaaaatat
      481 agtcgtgaaa atgggcagta tatggctttt attccttcaa aatttaagga aaaggaagtc
      541 ttcccaagtg gtacctttga taaaacaatc gatttaccct ttgagaattt aagccttcct
      601 gcacctgaaa aatttgatac tattttgaca caattttatg gagattatat gaccctacca
      661 ccagaagaaa aacgcttcta cagtcatgaa tttcacgctt ataaattgga ggattaggat
      721 gcaatattta gaaaaaaaag aaattaaaga aattcaacta gccctgctgg actatattga
      781 tgagacttgt aagaaacatg atattcctta ttttctcagt tatggaacca tgcttggagc
      841 catccgccac aaaggtatga ttccttggga tgatgatatt gatatttccc tttatcgtga
      901 ggattatgag cgtttactga agattattga agaagaaaat caccctcgct acaaggttct
      961 ttcctacgat acatcttctt ggtacttcca taatttcgca tcgattttgg acacttctac
     1021 tgttatagaa gaccatgtta agtacaagcg tcatgatacc agccttttca tcgatgtctt
     1081 cccaattgat cgatttacag acttgagcat tgtcgacaag agctataagt atgtggccct
     1141 tcgtcaacta gcttatatca aaaaatcacg agcagttcac ggtgatagca aactaaaaga
     1201 ttttcttaga ttatgtagct ggtacgctct ccgatttgtc aatcctcgct acttttacaa
     1261 gaaaattgat caactagtca aaaatgctgt aaccaacact cctcaatatg aaggaggagt
     1321 tgggatcggt aaggaaggga tgaaagaaat cttcccagtt gataccttta aagaactgat
     1381 tttaactgag tttgagggcc gtatgttgcc tgttcccaaa aaatatgacc aatttttaac
     1441 ccagatgtat ggcgattata tgacaccacc atcaaaagaa atgcaagagt ggtatagtca
     1501 tagcattaaa gcttatcgca aaaactgatt gagggggatt atacaaacta ctaagataga
     1561 ggttattcaa aaacataatt ttagtagaaa atg
//
LOCUS       DAVLYR010000218         1584 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVLYR010000218 DAVLYR010000000
VERSION     DAVLYR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.218"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            158..634
                     /locus_tag="VYO63_001952"
     CDS             158..634
                     /locus_tag="VYO63_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1672796.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            634..1395
                     /locus_tag="VYO63_001953"
     CDS             634..1395
                     /locus_tag="VYO63_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1672797.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSGENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          587 a          214 c          310 g          473 t
ORIGIN      
        1 catcgaaaaa attaatacaa gaataaatat ttatagctct ttaggtgact tttatagaag
       61 taaagtttag gatagaaaaa caagaaataa cgcaccattt ttggtgcgtt atgctttttt
      121 atgctataat ggatttataa aaataaagga gtttgctatg attggaaaga acataaaatc
      181 cttacgtaaa acacatgact taacacaacc cgaatttgca cgaattatag gaatttctcg
      241 aaatagtctg agtcgttatg aaaatggaac gagttcagtc tctaccgaat taatagacat
      301 catttgtcag aagtttaatg tatcttatgt cgatatcgta ggagaagata aaatgctcaa
      361 tcctgttgaa gattatgaat tgactttgaa aattgaaatt gtgaaagaaa gaggtgctaa
      421 tctattatct cgactctatc gttatcaaga tagtcaggga attagcattg atgatgaatc
      481 taatccttgg attttaatga gtgatgatct atctgacttg attcagacaa atatctattt
      541 agtagaaaat tttgatgaaa tagagagata tagcggctat ttggatggaa ttgaacgtat
      601 gttagagata tctgaaaagc ggatggtagc ttaatggaaa tccaagatta tactgatagt
      661 gaattcaaac atgctttagc aaggaatctt cgttcactga caagaggaaa aaagtccagt
      721 aaacaaccta tagcgatttt gcttggaggt caaagtggtg ccggtaagac tacaattcat
      781 cgtattaaac agaaagaatt tcaaggaaat attgttatca tagatggtga tagttttcgt
      841 tctcagcatc cacactattt agaactgcag caagaatatg gcaaagacag tgtagaatat
      901 accaaagatt ttgcaggaaa aatggtagag tctttagtaa caaaattgag tagtttgaga
      961 tacaatcttt tgatcgaggg aactttacga acagttgatg ttccaaagaa aacagcacaa
     1021 ctcttgaaaa ataagggata tgaagtacaa ttggccttaa ttgcgacaaa gcctgaattg
     1081 tcgtatctaa gtactcttat ccgttatgaa gaactgtaca ttatcaatcc aaatcaagca
     1141 cgcgcaactc caaaagaaca tcatgatttc attgtaaatc atctagttga taatacacga
     1201 caattggaag aactggctat ctttgaaaga attcaaattt atcaacgaga tagaagttct
     1261 gtatatgatt caggagaaaa tacaacttca gcagcagatg ttcttcaaga gttactgttt
     1321 ggggagtgga gtcaggtaga gaaggagatg ttgcaggtgg gggaaaagag acttaatgaa
     1381 ttacttgaaa aataaacaat tgatattttt aggagagtag aaatgagagg gtttaataac
     1441 aagataaagt ctgtttatca agaactaaca aattccaaag agaaattcgg tagctttcac
     1501 aagactttaa ttcatttgca tacacctgtt tcttatgatt acaagctatt ttctaattgg
     1561 actgcaacga aatatagaaa aatt
//
LOCUS       DAVLYR010000219         1582 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVLYR010000219 DAVLYR010000000
VERSION     DAVLYR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.219"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            20..304
                     /locus_tag="VYO63_001954"
     CDS             20..304
                     /locus_tag="VYO63_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672798.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDIEKL
                     LDSKELTHFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            297..854
                     /locus_tag="VYO63_001955"
     CDS             297..854
                     /locus_tag="VYO63_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter peripheral membrane
                     component"
                     /protein_id="HEV1672799.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNTHEELWLNNHVANMNKQPPLSLCESLYSFENLTVL
                     VVPIEYVLGMKMMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVLLEG
                     FSYAYGMDWLEKFFKENQDKLREFY"
     gene            1344..>1582
                     /locus_tag="VYO63_001956"
     CDS             1344..>1582
                     /locus_tag="VYO63_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672800.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRR"
BASE COUNT          562 a          273 c          264 g          483 t
ORIGIN      
        1 atgccatagg agaaagacaa tggattggta cgattatatg atacaggcat ccaaacaatc
       61 acaattcaac gcaagccatt ggtttcgcta tttgcgaaaa gttatttttg aagactattc
      121 ttatttaaca aaccaagata tagaaaagtt gctagactcc aaagaactaa cccattttca
      181 aaaaattagc ttgaagtatg cctttcaaga gcatactcca actcataaat atgtgatttc
      241 attaaataaa cctgctaagt taaccaatgt tcaaaaattg atggagaaat acaagcatgg
      301 ataaaatgaa accggtcttc caagcactaa ataaggaatt aattcaggaa aatctgactt
      361 taacaattat ctgtgtcggt ggttatgtct tagaatatca tggtttgcgt gccacacaag
      421 atgttgatgc tttttatgac caaaatcaga aaatcaatga aattattgct cgtgtaggaa
      481 aacagtttaa tcttaacacc cacgaagagt tatggctcaa caatcatgtt gcaaatatga
      541 acaaacaacc tcccttaagt ttatgcgaat ccttgtactc atttgagaat ctgaccgttt
      601 tagtagtccc tattgagtat gtgttaggaa tgaagatgat gagtatcaga gagcaggatt
      661 tgaaagatat tggtgcaatt attaaataca aaaactttca ttcaccattc gataccttta
      721 aatatttaaa agacatggga tttgatacta ttgacctctc tgttctatta gaaggtttta
      781 gttatgctta tggtatggat tggttagaaa aattttttaa agaaaatcaa gataaacttc
      841 gcgaatttta ttaacagtat caaacaagat gattgtcaat tagccagtcg tcttgttttt
      901 atgatgaaat gaaataaaat cggaacaaat cacttagaga attccagtca atttctagca
      961 ctgtttgaga cgtttcagcg gactatagca aaatgaaata agaataggac aaatcgatca
     1021 ggacagtcaa atcgatttct aagaatgttt tagaagtaaa ggtgtactat tctactttca
     1081 atctattata tttcagattc ggtccactat tccttcccaa aaacattgca tattgcgccg
     1141 aaggaattgc tcatactatc ccaagcacaa aaccctcagt cgtttgaccg agggttcctt
     1201 tttattattc aagaacttga ggctctataa tatttgtagt gggtaaatcc cctatggata
     1261 ttatggagcc tattttgttg tagaaaaaaa gtcccatatg acctataatg aaaagcgaca
     1321 aaaccatcat tagaaagatt catatggaac aattacattt tatcacaaaa ctgctcgata
     1381 ttaaagaccc aaacatcaag attctagata tcatcaatat ggatacccac aaagaaatta
     1441 tcgctaagct ggattatgag gctccatctt gccctgattg tggaagtcta atgaagaaat
     1501 atgactttca aaaaccgtct aagatccctt acctcgaaac aactggtatg ccttctagaa
     1561 ttctccttag aaaacgccgt tt
//
LOCUS       DAVLYR010000220         1581 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVLYR010000220 DAVLYR010000000
VERSION     DAVLYR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.220"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..203)
                     /locus_tag="VYO63_001957"
     CDS             complement(<1..203)
                     /locus_tag="VYO63_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672801.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFI"
     gene            complement(1339..>1581)
                     /locus_tag="VYO63_001958"
     CDS             complement(1339..>1581)
                     /locus_tag="VYO63_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672802.1"
                     /translation="IKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYL
                     LVIQKAGYLFSVLLLFSNFSVPFIKAKEYELFKNSK"
BASE COUNT          572 a          290 c          210 g          509 t
ORIGIN      
        1 aaaataaaaa caccagcttg aatagcaggt tttgtttgac tagcataatc tcgtgtgatt
       61 ttgtataagt caaataaatg atgtaaatca gtcaagcaaa aattcacttt gaaaatctct
      121 aaatacttat aattcgttga tacttgacat gtttttccat tgaatctttc ttgatagtcc
      181 aaaatacaat caagtaattt caacgacaaa ctccatattt tctcaaaata aaaagcacgg
      241 ggcagttctc actactgccc aaagtggttg attagagtcg gcttctctaa ctccctatgt
      301 tgtgcttatt aggcttatcg caagggaaca gtttttagct ctgccaagcc acatagtgtg
      361 cttactacca tgtgtccatg tcaccatgaa ttgccgaacg gtttaacgag aaagtatagt
      421 agattgaaac aagatgtgaa caaatcgatt aggaaagtca aattaatttc tagaaatatt
      481 ttaacagtca taacggacta ttccagattc aatctactat attactttta ggtttatccc
      541 aactcaatta tgacatcttt ttttcaaaag tcaatatatc tcactctttt caacgacaag
      601 aaagaggctg ataatcgacc aacctctgtt tttagataca gttcaagtcc aaactattct
      661 ttatagaaaa aagtcctata aattcgagca atagttaaca tacttacaaa tataactccc
      721 aaaatagata acactatttt cacaaaactc agttccgaac ctatgacata tatatgaaaa
      781 agaggggcta gtcctacaca tatatacaat aagtagccaa caagttttat ctcaccaatt
      841 cctgcaaaaa aatgtttcat gtgacgattc tccatttact ccattaaatc agttttattt
      901 tagactatca taaaacaatc acctcttcaa taaaaaccat ttgcgaaata ctattctgaa
      961 gcattgatag ctcagatttc tcagaataga gctgtcctaa ctatcgagca gctcaaatac
     1021 atatatatga gcaattctaa gcctagaatt gctataaaat aaatgtttcc aggctattct
     1081 aagtctttga ctgcttaaaa ttttacattc tgaaggattc taaagctaga acagctcaaa
     1141 atcacgagaa ctaaggtatt cgaaacttca gagtaataca atccccatag gaattctcca
     1201 ttcttattaa gcatgattaa aatgtaatcc taacaagtga tcctcgactg tgtttgtagc
     1261 acttagttac ctagataaga ctatttccct ttatcatatc tgattttaag ccgttctaag
     1321 atttaaccta tgtttctatt acttgctatt tttaaataat tcgtattcct ttgctttgat
     1381 aaatggaact gaaaaattag aaaaaagaag taaaaccgaa aacaaatagc ctgctttttg
     1441 aatcactaaa agatatccta aaaataagta agctataaca tccaatgcga aaagaaaaaa
     1501 tattctcctc ttcattaaag tacttgtgtc aaataatctg atgaataaaa attttatcgt
     1561 agtatctaca caaattttta t
//
LOCUS       DAVLYR010000221         1536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVLYR010000221 DAVLYR010000000
VERSION     DAVLYR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.221"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            200..1483
                     /locus_tag="VYO63_001959"
     CDS             200..1483
                     /locus_tag="VYO63_001959"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1672803.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT          542 a          277 c          317 g          400 t
ORIGIN      
        1 actgaaaaat tagataaatt agttttggaa aatgactaac caaaagatat ccaaagtagt
       61 ctaaaattgt ctatacttta tgagtgtttt agttaggaaa aaggcttgtt gtctataatt
      121 ggctgcatta gtctagattt tatttataga aaatgttata atagactgta tttaaaaaat
      181 tttaaggaga aatgacagaa tgtctgtatc atttgaaaac aaagaaacaa accgtggtgt
      241 cttgactttc actatctctc aagaccaaat caaaccagaa ttggaccgtg tcttcaagtc
      301 agtgaagaaa tctcttaatg ttccaggttt ccgtaaaggt caccttccac gtcctatctt
      361 cgataaaaaa tttggtgaag aatcacttta ccaagacgtt atgaacgctc ttttgccaaa
      421 cgcttatgaa gcagctgtaa aagaagctgg tcttgaagtg gttgcccaac caaaaattga
      481 cgtaacttca atggaaaaag gtcaagactg ggttatcgct gctgaagtcg ttacaaaacc
      541 tgaagtaaaa ttgggtgact acaaaaacct tgaagtatca gttgatgtag aaaaagaagt
      601 aactgacgct gatgttgaag agcgtatcga acgcgaacgc aacaacctgg ctgaattggt
      661 tatcaaggaa gctgctgctg aaaacggcga cactgttgtg atcgacttcg ttggttctat
      721 cgacggtgtt gaatttgacg gtggaaaagg tgaaaacttc tcacttggac ttggttcagg
      781 tcaattcatc cctggtttcg aagaccaatt ggtaggtcac tcagctggcg aaaccgttga
      841 tgttatcgta acattcccag aagactacca agcagaagac cttgcaggta aagaagctaa
      901 attcgtgaca actatccacg aagtaaaagc taaagaagtt ccagctcttg acgatgaact
      961 tgcaaaagac attgatgaag aagttgaaac acttgctgac ttgaaagaaa aataccgcaa
     1021 agaattggct gctgctaaag aagaagctta caaagatgca gttgaaggtg cagcaattga
     1081 tacagctgta gaaaacgctg aaatcgtaga acttccagaa gaaatgatcc atgaagaagt
     1141 tcaccgttca gtaaatgaat tccttgggaa cttgcaacgt caagggatca accctgacat
     1201 gtacttccaa atcactggaa ctactcaaga agaccttcac aaccaatacc aagcagaagc
     1261 tgagtcacgt actaagacta accttgttat cgaagcagtt gccaaagctg aaggatttga
     1321 tgcttcagaa gaagaaatcc aaaaagaagt tgagcaattg gcagcagact acaacatgga
     1381 agttgcacaa gttcaaaact tgctttcagc tgacatgttg aaacatgata tcactatcaa
     1441 aaaagctgtt gaattgatca caagcacagc aacagtaaaa taatcttaat aaacagaaaa
     1501 cccacctgaa ttggtgggtt ttctgatgta ctattt
//
LOCUS       DAVLYR010000222         1515 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVLYR010000222 DAVLYR010000000
VERSION     DAVLYR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.222"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            76..1500
                     /locus_tag="VYO63_001960"
     CDS             76..1500
                     /locus_tag="VYO63_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1672804.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVAEKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
BASE COUNT          462 a          274 c          350 g          429 t
ORIGIN      
        1 aattttgcaa aaagggttgt gattttctga aaatttggta aaatatatct taatcatttt
       61 caggaggaca agaatttgac aagatatcag aatttagtaa atggaaaatg gaaatcatct
      121 gaacaagaaa ttacgattta ttcaccaatc aatcaagaag aattgggtac agttccagcc
      181 atgactcaga ctgaagctga tgaggctatg caagctgcgc gtgcagccct gccagcatgg
      241 cgagctttat cagcagttga acgtgcggct tatttacata aaacagcagc tattttagaa
      301 cgcgataagg aagaaattgg tactatcctt gccaaagaag tagcaaaagg gattaaagca
      361 gcaattggag aagtagtgcg tacagcagac ttgattcgtt atgctgctga ggaaggtctc
      421 cgtatcactg gacaagcaat ggaaggtggt ggttttgagg cagcaagtaa aaacaaactg
      481 gctgttgtcc gtcgtgaacc agttggtatc gtgctagcga ttgctccctt taattatcca
      541 gttaatttat ctgcttctaa aattgcacct gccttgattg cagggaatgt ggtcatgttt
      601 aagccaccaa cacaaggttc catttctgga ctcttgttgg ctaaagcatt tgaagaagca
      661 gggattccgg caggtgtttt caacaccatt acaggtcgtg gttcagaaat tggggattat
      721 atcattgagc acaaagaagt caacttcatc aactttacag gttcaactcc tattggagaa
      781 cgtattggtc gtttagctgg tatgcgtcct atcatgttgg aacttggtgg gaaagatgca
      841 gctcttgtac tagaagatgc agatttggaa catgctgcca agcaaattgt tgcgggagcc
      901 tttagctact caggacaacg ttgcacggcc attaaacgtg tcattgttct cgaaagtgta
      961 gcagagaaat tagctacttt gcttcaggaa gaagtttcta aattaacagt tggtgatcca
     1021 tttgacaatg ctgatattac acctgttatt gacaatgctt cagccgactt catttggggc
     1081 ttgattgagg atgcacaaga aaaagaggct caggctctta caccaatcaa acgtgagggc
     1141 aatctcctct ggccagtgct ttttgaccaa gttacaaaag atatgaaagt ggcatgggaa
     1201 gagccatttg gtcctgtttt accaatcatt cgtgtggcta gtgtagagga agctattgcc
     1261 tttgccaacg aatctgaatt cggccttcaa tcatcagtct ttacaaatga tttcaaaaaa
     1321 gcctttgaaa ttgctgaaaa acttgaagta ggtacagtcc acattaataa taaaacccag
     1381 cgtggtccag ataatttccc attccttggt gtcaaaggtt ctggagctgg agtgcaagga
     1441 attaaatata gcattgaagc gatgacaaat gtcaaatcca ttgtttttga tgtgaaataa
     1501 cgtgtaaaat cagga
//
LOCUS       DAVLYR010000223         1513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVLYR010000223 DAVLYR010000000
VERSION     DAVLYR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.223"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..662
                     /locus_tag="VYO63_001961"
     CDS             <1..662
                     /locus_tag="VYO63_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1672805.1"
                     /translation="IDCMAIGQYLFNPHNTPDNFIFLHAGLGIYTSFFTKEKIGASKN
                     PYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFKNSQLTVLKSLVKTE
                     KDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPADKIYINSQLLNYQPF
                     THEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVAFFIEHSNVLQDIIS
                     P"
BASE COUNT          568 a          284 c          207 g          454 t
ORIGIN      
        1 atatcgattg tatggctata ggacaatacc tttttaaccc acacaatacc cccgataact
       61 ttattttcct acacgctgga ttaggtattt acacttcctt tttcacaaaa gaaaaaatag
      121 gagcctctaa aaatccttat atcggagaaa ttggacacac cattgtcgaa ttaaatgggc
      181 aatattgtga atgcggaaaa aaaggttgtt tacaaacata tatttcggat gcttggttaa
      241 tcaaacatgc ccaattatta tttaaaaatt ctcaactaac tgtactaaaa agccttgtaa
      301 agactgaaaa agacattcat ttagataccc ttttaacagc ttacaactta ggcgactctg
      361 ctttacgcca acaaattgat aaaggagtca atttattggc cacttctatt gcaaatcttc
      421 tcctcatcaa tcctgctgat aaaatctata tcaacagtca attgcttaat tatcaacctt
      481 tcactcatga agtcagggat aaaatccaag atcagctcca cttcgttccc tttactcgta
      541 atatagaaat tgaaatttta ccttacaaca aacatcgtgg aagtatagga gcttgtgcat
      601 tagctatcgt tgcttttttc atagagcata gcaatgtatt acaagatatt atttcacctt
      661 aatatattag aaatctatag acctgtttaa atcaactata acctgtagta aatatctcgt
      721 atttagacaa tatgaaaaca agacgacttc catataggaa accgccttct cgctatgctg
      781 catgaaactg taaaaataga tgaaagtgat agcggtagtc aaatcaagag gcatcataac
      841 tctaaaaagt cacaatatat aagttcatcc tcagaaaaat atcattctaa ttattgaaat
      901 gcctacatga aaagaaacgt taaatgctca taaaacaagg aatacaggta tcaaaactgt
      961 gacaaaacaa gtccctaaat ttactaaaga cactactcaa ctttacacct gtaaatggtt
     1021 gttgtataat aaagttacaa agatgtacga ccacactgtt gtaaatcata gtgttcgcga
     1081 atatattact gatagcattt ctacaaatac aagtaaaggg attggatgag attcaaacga
     1141 aatatgtcag tgttttggca ttcctagcct tcatatcatt taaagaattg tatagataaa
     1201 atttttccca atacagacac tcgtaacaac tgcttcattt ttctaccaac atatttagga
     1261 acaggataag atacaagagg atcaatccat aaatcagttc tataccaatc taagacaaat
     1321 aagctaaaaa aacgattgat aataagcaaa tagattccaa attttctcta tctgctcatt
     1381 ttaataaaca atactagtgt aactatcctt ccagtcagaa gcttgtcaaa tcacaccgaa
     1441 aattcttcta aaatttatct cgttaggcaa tcaagcaaaa actcaacgat agtacaaaca
     1501 ttatcataca gga
//
LOCUS       DAVLYR010000224         1502 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVLYR010000224 DAVLYR010000000
VERSION     DAVLYR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.224"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            25..1479
                     /gene="tacF"
                     /locus_tag="VYO63_001962"
     CDS             25..1479
                     /gene="tacF"
                     /locus_tag="VYO63_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1672806.1"
                     /translation="MGIRVLNIIFPILTGTYVARVLDRTDYGYFNSVDTILSFFLPFA
                     TYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILAYPLFFIDNPIV
                     KKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIFLFVKNEHDIVV
                     YTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVFANANMLFTFLD
                     RLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGKGDKEAYVSLVN
                     RGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRTIILALDTILGS
                     QILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTMLSETSLLVFYII
                     FIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLPFLINTGLIVLL
                     SAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
BASE COUNT          398 a          306 c          242 g          556 t
ORIGIN      
        1 ataaaattaa atgctctatc ttacatggga attcgtgtct tgaatattat ttttcccatc
       61 ctaactggaa cctatgtcgc gcgtgtcttg gaccgaactg actatggtta cttcaactca
      121 gtcgacacta ttttgtcatt tttcttgccc tttgcaactt atggtgtcta taactacggt
      181 ttaagggcta tcagtaatgt caaggataac aaaaaagatc ttaacagaac cttttctagt
      241 cttttttatt tgtgcatcgc ttgtacgatt ttgaccactg ctgtctatat cctagcctat
      301 cctctcttct ttattgataa tccaatcgtc aaaaaggtct accttgttat ggggattcaa
      361 ctcattgccc agattttttc aatcgaatgg gtcaatgaag ctctggaaaa ttacagtttt
      421 ctcttttaca aaactgcctt catccgtatc ctgatgctgg tctctatttt cttatttgtt
      481 aaaaatgaac acgatattgt tgtctataca cttgtgatga gtttatcgac gctgattaac
      541 tacctgatta gttatttttg gattaaaaga gacatcaaac ttgttaagat tcacctaagt
      601 gattttaaac cactctttct ccctctgaca gccatgttag tctttgccaa tgccaatatg
      661 ctcttcactt ttttagatcg cctcttcctc gttaaaacag ggattgatgt caacgttagt
      721 tactatacca tagctcagcg aattgtgacc gttatagctg gggttgtaac aggtgcaatt
      781 ggagtgagtg tgcctcgtct cagttactat ctggggaaag gagacaaaga agcctatgtt
      841 tctctggtta atagaggtag tcgaatcttt aacttcttta tcattccact gagttttgga
      901 ctcatggttt taggaccaaa tgccatccta ctttacggta gtgaaaaata tatcggaggc
      961 ggcatcttga cctctctctt cgcttttcgt acgattatcc tggccttaga taccattctt
     1021 ggttcccaaa ttctctttac caatggctat gaaaaacgta tcacagtcta tacagtcttt
     1081 gctgggctac tcaatttggg cttgaatagt ctcctctttt tcaaccatat cgtggttcct
     1141 gaatactact tactgacaac tatgttatca gagacttctc tacttgtttt ctatattatt
     1201 ttcatccata gaaaacaact catccacttg ggacatatct ttagctatac tgttcgatac
     1261 tctctctttt cactttcctt tgtagcaatt tatttcctga ttaatttcgt gtatcctgtg
     1321 gatatggtca ttaatttgcc atttttaatt aatactggtt tgattgtctt gctatcagct
     1381 atctcttata ttagtctact tgtcttcaca aaagatagca ttttctatga atttttaaac
     1441 catgtcctag ccttaaaaaa taaattcaaa aaatcatagg agtttaaaat gaaacaacta
     1501 ac
//
LOCUS       DAVLYR010000225         1477 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVLYR010000225 DAVLYR010000000
VERSION     DAVLYR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.225"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            432..1433
                     /locus_tag="VYO63_001963"
     CDS             432..1433
                     /locus_tag="VYO63_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1672807.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
BASE COUNT          556 a          199 c          275 g          447 t
ORIGIN      
        1 aaataatata aacacggtct cactcatata cttgttaaat gccatgatgt ttattcgatt
       61 agatgagtaa ccaaaaataa caaaaaaacg ctatttcgcg tttgaactgt acctcaaaag
      121 ttagacagaa aaaatctaat ttttggggtg tttttgttaa gaaaagacct tcaatcattt
      181 aagcaaaatg tacatctaga tttatagccc tctaaaactc ttgctttttt tgtagaagat
      241 acgaaaaaag ccataaaacc aaggcttttc ctgttttatg tagatgttta cacatgaaaa
      301 ttgaagtatt ttcaaaagct aattggagtg attttattca tgatgggata aatgatctgg
      361 ccaaaaatga ttgagaaata gttgcttaaa aagcatttgc atgatactat atattgaaag
      421 gaggtgataa tatggtagaa caaagaaaat caattaccat gaaagatgtt gctttagaag
      481 caggagttag tgttggaact gtttcacgtg taattaataa agaaaaaggc attaaagaag
      541 taactttgaa aaaagtggaa caagcgatta aaactttgaa ttacattcca gattactatg
      601 ctagaggaat gaaaaaaaat cgaacagaaa cgattgcaat cattgtacca agtatctggc
      661 atcccttctt ttcagaattt gctatgcatg tggaaaatga agtctataag agaaataaca
      721 aattactctt atgttctata aatggtacaa atagagagca agactatctg gagatgttgc
      781 gtcataataa agttgatgga gtggttgcca ttacctatag tccaattgaa cattacttga
      841 cgtcaggaat tccctttgtt agtattgacc gcacatactc agatattgcc attccttgtg
      901 tttcatcaga taatgatgca ggtggaagag aagcagctaa acaattaatt agtaaaggat
      961 gtcagcattt agcctttgtg ggcggacata atacaaccat taatgaaacc aaacgtcgta
     1021 gaatttcatt tgaaaagtat gtccaagagc ataaaatacc tcttagtatt tttgatttgg
     1081 atgagacagt tactgactat tatggaaagc tagagagttt cttggaagaa aattcttcta
     1141 tagatggaat ttttacaatc aacgatttta cagctttgga tgtgattgaa atactagaaa
     1201 aaaatggcaa acgtattcct catgatgtcc aaattattgg atacgatgga attaaaatgg
     1261 ctggagatag agattatcta ctttcaacta ttgaacaacc attggaagaa atggcaaaag
     1321 aagcggttcg tattttgttt gatataattg atggaaagac tgttaatttg cagacaatcc
     1381 taccagtaaa atttgttgaa ggaaaaacaa caaaaaatga aaataagtct tgacagaaag
     1441 cgctatcaat gatagaatga attcagataa aaagatt
//
LOCUS       DAVLYR010000226         1473 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVLYR010000226 DAVLYR010000000
VERSION     DAVLYR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.226"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(188..559)
                     /locus_tag="VYO63_001964"
     CDS             complement(188..559)
                     /locus_tag="VYO63_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672808.1"
                     /translation="MIKKNHILCEIVPGAVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            complement(1028..1147)
                     /locus_tag="VYO63_001965"
     CDS             complement(1028..1147)
                     /locus_tag="VYO63_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1672809.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(1251..1400)
                     /gene="blpO"
                     /locus_tag="VYO63_001966"
     CDS             complement(1251..1400)
                     /gene="blpO"
                     /locus_tag="VYO63_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1672810.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
BASE COUNT          545 a          293 c          187 g          448 t
ORIGIN      
        1 tcttatccat aattatttac tcctttccta acaaatccaa cttatcaatc aagagcgatt
       61 tttaacataa tgtagcagca cccgttgcaa ctttgacaag tttagtatat cattgttttt
      121 taaaattttt catccaaatc ttgaattgtc atcgaaacct cctaaattgc aaaaacttca
      181 ttagccccta ctttttataa tacttcaaac cccaaatgag tagaagcatg atgatcaagc
      241 aaagaatagc accaatataa gcgattagtt gttgataaga ttctcctgct gtgatacctc
      301 tatacaaaca aataatagac ataaagcctg tcaagccgat aaacatgagt tgattggttc
      361 taggactaac caaatcatcg tcttcaaact cttttatccg catttcccta gtgaggtaaa
      421 cagtgaccaa aatagaagct aagttaataa ccactaaaag aaattggaaa actgacgaaa
      481 tagaaataga taagtagaaa caagcaaagg caactgcccc aggaacaatc tcacaaagaa
      541 tatgattctt tttaatcaag aaaatctttc atttcttttc tttcttttta attaaagaaa
      601 aatagatcat aaccgcaatc atataggcta tggtataaaa tagattatac caaatactct
      661 ccctaagcgg ataaagaaag atggacatga tcagatacag aacgaaaata atcagtattt
      721 ttttcttcat aagatttcct cctaaatgtg cgatttatct tagttgagca agaacattta
      781 cactgctagt atagcactta ttttgacctt ggatcactca aatcataaat ggtcatcaaa
      841 acctcttgaa ttgtaaaaat taaaaaaagc aagcatgaaa aacatacttt cctctttatg
      901 ttgtattgat accaacttgt ttgtagactt ttcatcctgc tatcacatat cattttgaca
      961 ggcgaaacaa tattaaagaa actcccctgt aaattaagct agcaaataca ggggagaaat
     1021 ttatttttta gagagtacta tccgtatcct ttttggaaga ttttgaaaat atttttctaa
     1081 ttaagtcatc catataagga ccaaatatac caactactaa accaataata aaacttttaa
     1141 aatccataat taccaccaac atgttgctac ataggctaca cctccaagta tagctccacc
     1201 tgcagcacca gttacaccta ttcctatagc aaatggtccc aatagaaatg tcaaaccgtt
     1261 gttgcacacc catcaattgc gccatatgca acccctgctg cacaactaat ttttcttccc
     1321 caatcaatat ctccaccttc aacgcaagca agcatttcat tatccataac agaaaattgt
     1381 gacatcattt ttgtattcat atagtatgtc acttttcagt tacggaacaa gtttaatata
     1441 aaaattatca aaaaaacata ggcaataaag aga
//
LOCUS       DAVLYR010000227         1461 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVLYR010000227 DAVLYR010000000
VERSION     DAVLYR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.227"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(279..1235)
                     /gene="lytA"
                     /locus_tag="VYO63_001967"
     CDS             complement(279..1235)
                     /gene="lytA"
                     /locus_tag="VYO63_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1672811.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADRPEFTVEPDGLITVK"
BASE COUNT          389 a          338 c          264 g          470 t
ORIGIN      
        1 aaggaagcaa tttattgatg ctgcttaaaa atctaaaatt gatgagttcc tatcaaaaat
       61 cgtatcttca acctcaaaac agtacttaaa gctatccgac tcggtttaca ttgtcaaatt
      121 tagattttat ttgagcataa ctttctagtt tgctttctga tttttgttta atatagtagc
      181 aaacattgaa ggaaatatct ccacaagaaa acgcatacta ttaagctttt tcaagaccta
      241 ataatatgcg ctgttctgat ttgaaagaca ttccattatt attttactgt aatcaagcca
      301 tctggctcta ctgtgaattc tggcctgtct gccagtgttc cgtctggttt gaggtagtac
      361 cagcctgttc cgtccgctga ctggataaag gcatttgata ccatggcgcc ttctttagcg
      421 tctaagtagt accaagtgtc cttgtacttg acccagcctg tcttcatggc accttcttcg
      481 ttgaaatagt accacttatc agcgattttc ttccagcctg tagccatttc gcctgagttg
      541 tcgaaccagt accagttgcc gtctgtgtgc ttcctccagc ggtctgcaag catatagcct
      601 gaactgtcaa agtagtacca agtgccattg attttctcaa acttgtcttt tggataagag
      661 ccgtctgaat gtacgtacca gtagccagtg tcattcttct gccagcctgt ttcaatcgtc
      721 aagccgttct caatatcatg cttaaactgc tcacggctaa tgccccattt agcaagatat
      781 ggataagggt caacgtggtc tgagtggttg tttggttggt tattcgtgca atactcgtgc
      841 gttttaattc cagctaaact ccctgtatca agcgttttcg gcaaacctgc ttcatctgct
      901 agattgcgta agagttcgat ataaaggcgg tagtccgtca tgaactcttc tttggttgaa
      961 tggctttcaa tcagttcaac cgctgcatag gtctcagcat tccaaccgcc cccaacgtcc
     1021 caggcaccat tatcaacagg tcctacctgc atgatgcaac cgttcccaac aatgtgcgag
     1081 aaaaaaccta attctgggtc tttccgccag tgataatccg cttcattctg tacggttgaa
     1141 tgcggattcc cagttgagtg tgcgtgtact tgcctatatg gttgcacgcc gacttgaggc
     1201 aaatctgttc ttaatttact cacattaatt tccatattct actccttatc aattaaaaca
     1261 actcattttt tacaatccag aaccagaaac tcctttattt ctaccttaca aagaagacaa
     1321 tcttagtcac gattaggctt gtagatagaa cttcaaaacg cactattttg acactgtaaa
     1381 taggactgac aaggtctgca ttctatctac aataacaccc cagactaaaa agcttttcaa
     1441 aagtatattt ttacagtctc t
//
LOCUS       DAVLYR010000228         1449 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVLYR010000228 DAVLYR010000000
VERSION     DAVLYR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.228"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            2..796
                     /locus_tag="VYO63_001968"
     CDS             2..796
                     /locus_tag="VYO63_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672812.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            800..997
                     /locus_tag="VYO63_001969"
     CDS             800..997
                     /locus_tag="VYO63_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672813.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            987..>1449
                     /locus_tag="VYO63_001970"
     CDS             987..>1449
                     /locus_tag="VYO63_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672814.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCG"
BASE COUNT          482 a          207 c          278 g          482 t
ORIGIN      
        1 catgaatgtc aaaaagataa tgtcaatttt tcaatccttt tatgttgatg tcagtattga
       61 ggaactgact ttgactttac caatcagttt tgtaaaaagg tttgagtata ctcaaatgac
      121 ttttcataag gaatcatttt tattgattaa agaaaagaga agggggagtt tgagttcatt
      181 tgttactcag gctcgcacta tgggtgaaaa agccaatatg gatgttgttt tggtgttttc
      241 gaagttatca gacagtgaaa aaaagcaatt acttcaagct agagttccgt ttgtagactt
      301 taagggaaac ctcttcttcc ctccattggg actagtactc aatgcgaatg atactgaagt
      361 ccctaaggaa ttaacaccta gcgaacaatt aacgtggatt gcctttttat tgacaaaagg
      421 tcaaaaagta gtagatgttg atttgctttc acaagtcact ggacttccaa actcaacaat
      481 ttataggtgt ttgaggactt ttaaagcttt atattggtta aacaagcaaa ataagcttta
      541 cacatatacg gtgtcaaaga aagaattatt cttaaaatcc gtgtcatgtt tatttaatcc
      601 catcaaaaaa cggattttat tgccagatgg cgatataaag cagataaaat ctgtttctaa
      661 ccttctatat ggtggtgctt atgctttgtc gcattcaact tttttagctg aaacggatga
      721 aaatattagc tatgtcatat ggcagagaaa attcaatcag ttatccttgc cactttctca
      781 gcatgtttta aaatgaaaga tgctagagat atggaaatat cgtccttttg tatctgagtt
      841 ttggaatgat tttaaaaata atcatgataa acaatttgta gatccgattt ctctttattt
      901 gaccttaaaa gatgatgatg acccacgtat agaggaagag agtgaagcac tagaaaatat
      961 gatattacag tatctgggag aagatgatgc cagctaatac gaaagttatt tttcaagaaa
     1021 tgtttgcgga ttttcagaac tattatgttc tgattggggg aactgctacc tctatcgtat
     1081 tggattcgca aggatttaaa agtcgcacaa caaaagatta tgatatggtc atcattgatg
     1141 aagtaaaaaa taaggaattt tatactacct tgaatcattt tttagaattg ggagagtatc
     1201 aaggaagtca gaaagatgag aaagcgcagc tttttcgatt tacaacaact aattctgagt
     1261 ttccttctat gattgaacta tttagtatct taccagaata tccattaaag aaggacggtc
     1321 gagaaattcc cttatatttt gaccaagatg ctagtttatc agccttatta ttggatgaag
     1381 attattataa tatattggtg catgtaaaag aaaccattca ggggtattcg gtattgagta
     1441 attgtggtc
//
LOCUS       DAVLYR010000229         1443 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVLYR010000229 DAVLYR010000000
VERSION     DAVLYR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.229"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..667
                     /locus_tag="VYO63_001971"
                     /pseudo
     CDS             1..667
                     /locus_tag="VYO63_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(702..1443)
                     /locus_tag="VYO63_001972"
                     /pseudo
     CDS             complement(702..1443)
                     /locus_tag="VYO63_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          449 a          244 c          292 g          458 t
ORIGIN      
        1 gatagagata gacttaaaaa ctatcttact gacaatccag acgcttattt gactgaaata
       61 gcttctgaat ttggctgtca tccaactacc atccactatg cgctcaaagc tatgggctac
      121 actcgaaaaa aagaaccaca cctactatga acaagaccca gaaaaagtag ccttatttct
      181 taaaaatttt aatagtttaa agcacctagc acctgtttat attgatgaaa caggattcga
      241 tacttatttt tatcgagaat atggtcgctc attaaaaggt cagttaataa gaggtaaagt
      301 atctggaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaatg gtgagttaat
      361 cgctccaatg acttacgaag agatggtgac gagcgacttt tttgaagctt ggtttcagaa
      421 gtttctctta ccaacattaa ccacaccatc ggttattatt atggataatg caagattcca
      481 tagaatgggt aagttagaac ttttatgcga ggagtttggg cataaacttt tacctcttcc
      541 tccctactca cctgagtaca atcctattga gaaaacatgg gcttatatca aaaagaacct
      601 caaaaaggta ttaccaagtt gcaatacctt ttacgaggct cttttctctt gttcttgttt
      661 caattgacta tattagaggc gagacatttt tcggttcttt gtcaactgta gtgggttgaa
      721 gaaagcgaag atctagaaag gacaaatttc gtcctttctt ttttgaagtt ttcaaagttc
      781 ctaaaaccaa aggcattgtg cttgataagt ttgatgagat tattggtggc ttccagtttg
      841 gcgttggaat aaggtaattg aagggcgttg acgattttct ctttatcttt gaggaaggtt
      901 ttaaacagag tctgaaaaag aggtggaaaa gcaagagctg atagagatta tagctgatag
      961 agattatagt ggtgtttcaa gtcttcgaat agctcaaaag tttatctaga atttctttat
     1021 tagtcaagtg catacgaaaa gtagggcgat aaaatcgttt atcactcagt ttctgactat
     1081 cttgttgaat gagctccagt agcgcttgat agccttgtat tcatgggatt tcggatgatg
     1141 gcttgtgttc tgctctcaag aacagttatg atattgagct tatcaaagtc ctgagcaata
     1201 aagctcatct ctatctcccg attgaaacag tcactcccca gactgtttca acgtcctagg
     1261 acataatctc aggaagacgc gaaaaatcat gctcaaagtg aaaatcattg atcttgcgaa
     1321 tgacagttga agttgaaata gacaactgat gatcaatgtc ggtcatagaa atcttttcaa
     1381 ttaacttttg agcaatcttt tggttgataa tacgagggat ttggtgattc ttcttgacga
     1441 tag
//
LOCUS       DAVLYR010000230         1418 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVLYR010000230 DAVLYR010000000
VERSION     DAVLYR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.230"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            10..1356
                     /gene="gdhA"
                     /locus_tag="VYO63_001973"
     CDS             10..1356
                     /gene="gdhA"
                     /locus_tag="VYO63_001973"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1672815.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          420 a          293 c          312 g          393 t
ORIGIN      
        1 gagaacgata tgacatctgc taaagaatat atccaaagcg tgtttgaaac tgtaaaagct
       61 cgtaacgggc acgaggctga attcctccaa gctgttgaag aatttttcaa cactttggaa
      121 cctgtatttg aaaaacaccc tgagtatatc gaagaaaata tcttggcacg tattactgag
      181 cctgagcgcg tgatttcttt ccgtgttcct tgggttgacc gtgatggaaa agttcaagta
      241 aaccgcggct accgtgttca attcaactca gctgttggac catacaaagg tggacttcgt
      301 ttccacccaa ctgtaaacca agggatcttg aaattcctcg gatttgaaca aatctttaaa
      361 aatgtcttga ctggacttcc tatcggtgga ggtaaaggtg gatcagactt cgatcctaaa
      421 ggtaaaacag atgctgaagt gatgcgcttc tgccaaagct tcatgactga attgcaaaaa
      481 cacatcggac catcacttga cgtacctgct ggtgatatcg gtgttggtgg acgtgaaatt
      541 ggttaccttt acggtcaata caaacgtctt aaccaatttg atgctggtgt cttgactggt
      601 aaacctcttg gatttggtgg tagcttgatt cgtccagaag caactggtta cggtttggtt
      661 tactatactg aagaaatgct caaagctaac ggtaacagct ttgctggtaa gaaagtcgtt
      721 atttcaggtt ctggtaacgt tgctcaatac gctcttcaaa aagcaaccga acttggtgca
      781 acagttatct ctgtttctga ctcaaacggc tatgttatcg atgaaaacgg tatcgacttc
      841 gatcttttgg ttgatgttaa agaaaaacgt cgcgctcgtt tgactgagta tgcagctgag
      901 aaagcaactg ctacttatca tgaaggttct gtatggactt acgctggaaa ctatgacatt
      961 gcgcttccat gtgctactca aaacgaaatc aacggtgaag cagctaaacg tttggttgct
     1021 caaggcgtta tctgtgtatc tgaaggtgcc aacatgccaa gcaaccttga tgccatcaaa
     1081 gtttacaaag aaaatggtat cttctacgga cctgccaaag ctgccaacgc tggtggtgta
     1141 gccgtttcag cccttgaaat gagccaaaac agccttcgcc tctcatggac tcgtgaagaa
     1201 gttgatggac gtctcaaaga catcatgacc aacatcttta acacagctaa aacaacttca
     1261 gaaacatatg gtcttgataa agactacctt gcaggagcta acattgctgc ctttgaaaat
     1321 gtagcaaacg ctatgattgc acaaggcgtg gtataaaaaa aaattacgga aactgactac
     1381 ccagacgggt agccagtttt ttcaattcct aaaattta
//
LOCUS       DAVLYR010000231         1412 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVLYR010000231 DAVLYR010000000
VERSION     DAVLYR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.231"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            63..1241
                     /locus_tag="VYO63_001974"
     CDS             63..1241
                     /locus_tag="VYO63_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1672816.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          520 a          256 c          205 g          431 t
ORIGIN      
        1 catttatcca tagcaaaaaa tcataggatt ttaagaatct gacaactagg aagaaaacta
       61 atatgacctt atctaagaaa caattacaac taagagctaa aatactggaa acagtttaca
      121 cattaggtcc catttcacga attgaaatcg ctactaaaac aggaataaca cctgcaacaa
      181 ctagcagtat cacaaatgat ctgattaaag aaaatattct cttagaactt ggtgaagacg
      241 aacacgatac aagcgtaggg agaaaaaaaa tcctgctaga cattcaagct aaacgatttt
      301 actacattgg ttgtgaacta tctgaaaaac atttcacttt tgctttagga gataatctag
      361 gaaatatctt aaaggaagag aaagaaattg ttactaaaca gttaatccaa gaaaagggaa
      421 atcaactcat taaccagaca ctaaagcaat tcttaaataa ctgctctgat tatgaaatag
      481 aagcaattgg aatcgcgcta cctggtcgct atttagataa ttataaaata acaacaaaca
      541 atcctttatg gcaacatatc gacttagaaa tgattcaatc ccactttgat aagcctttgt
      601 ttttctctaa caatgttaac tgtatggcta taggtaaacg cctatttagt cgccaacaga
      661 atgaccctaa ttttgcttat tttcattttg ctagaggtat gcactgttct tatatctacg
      721 acggaaacat ttatgggaaa ggaaatctaa tgattggaga aatagggcat acagttgttt
      781 catctgaggg agaagaatgt agctgcgggc gcaagggatg cttacaaact tttgctggag
      841 aatcttggtt aatcaaaaaa tcaaaaattc tatatcatca atctccctat tctctacttc
      901 ctagtttagt caaaaatgcc gatgatattg atatccaagt tattttaact gcttatcaat
      961 taggtgacac tggaataatc acacttatcc atcaagcctt actctatctt agtcaaacca
     1021 ttttaaatat cagtatgatg attgactctc aaaaaatcta cctacatagt cctttactaa
     1081 caaatcaaca catcatccaa aaactatata gtgaaatgaa ctacaaaccc aaactgctct
     1141 ataatcgcct acctgaagtc attattgaac cttacaatga ttttacagcc gcacactcag
     1201 ctattgcact ttgtttgtat catacaattc tacattctta attttctatt acatttaata
     1261 aataggctaa aaagagggag tatatcataa aaagcgaata aacctgtttt ctgaaaatca
     1321 gaattctgtt ttgttcgctt tctttatatt tggaattata ctcctatctc ccctttttca
     1381 taatgtatag taaaatgaaa taataacagg ac
//
LOCUS       DAVLYR010000232         1379 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVLYR010000232 DAVLYR010000000
VERSION     DAVLYR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.232"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            29..1363
                     /locus_tag="VYO63_001975"
     CDS             29..1363
                     /locus_tag="VYO63_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1672817.1"
                     /translation="MKLWDLILLGIGAMVGTGVFTITGTAAATLAGPALVISIVISAL
                     CVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAGWLTMMEFMTAISGVASGWA
                     AYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLVLVTSLVLLNAKAALRFNSI
                     LVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGASTGIMAGASLMFFGFLGFE
                     SISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLTGVVHYSHLNVDDAVAFALR
                     SVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLARDGLVPAAFKELTKTSKIPK
                     NATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAYGLIRLRKEKGMPKAGEFKT
                     PLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSIIYFTYGYKHSTIEE"
BASE COUNT          325 a          269 c          304 g          481 t
ORIGIN      
        1 tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt tgctgggtat
       61 cggagccatg gtagggacag gcgtctttac aatcacaggt actgcagctg caacacttgc
      121 tggcccagcc ctagtgattt caatcgttat ttctgccttg tgtgtgggat tatcagccct
      181 cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata gttacctcta
      241 tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga tggagttcat
      301 gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct tgctctctca
      361 atacgggata gcctttcctc aggctttaaa tggtaccttt aatcctcaag cagggacatt
      421 tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt tactaaatgc
      481 caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg ctttagctct
      541 ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt ttgctcctta
      601 cggttttgga caaatctatg gagctagcac tggtattatg gctggtgcgt ccttgatgtt
      661 cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca agactcctca
      721 aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc tctatgcctt
      781 ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg atgatgccgt
      841 tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt cattagtggc
      901 tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc gtatgattta
      961 cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga agactagcaa
     1021 gataccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag caggaatgtt
     1081 cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact tgatcatgct
     1141 ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg gggaatttaa
     1201 aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat ccttcatgtt
     1261 acagtataat atgaacacct ggattgcttt cctagttgcg ttgttagtag gaagtattat
     1321 ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa gtctattga
//
LOCUS       DAVLYR010000233         1364 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVLYR010000233 DAVLYR010000000
VERSION     DAVLYR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.233"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(71..307)
                     /locus_tag="VYO63_001976"
     CDS             complement(71..307)
                     /locus_tag="VYO63_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1672818.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(375..1295)
                     /locus_tag="VYO63_001977"
     CDS             complement(375..1295)
                     /locus_tag="VYO63_001977"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1672819.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
BASE COUNT          394 a          245 c          264 g          461 t
ORIGIN      
        1 gataattgcg attttcattt tctacctttt cctatttgga gacaaaaaaa cagcctctat
       61 ggactgtttc ttatttagca agtttagctg aaagacgagc tttatcgcgg cttgctttgt
      121 ttttgtgaat caaaccttta gtttctgctt tatcgatagc tgagctagca gcacggaaaa
      181 gttcttcaga tgggtttgct tcgaaagctt ttatagcagt acgcatagct gatttttgag
      241 ctgagttctt ttcgttttgt ttaacgttca attcagcgcg tttgatagct gatttaatgt
      301 ttgccaatgt tcttacctcc atatttacta actataccat tatatatgaa aacttacgtt
      361 ttgacaaggg aaaattattt ttttaagtaa atttcatcga tttcatggtt cttgctttta
      421 tgaagaatca cttctgcacg atttctggtt ggttcaatat aattttgtag atttgtgaga
      481 ttgatactgg tccagacctg atgggcaaag gcttccactt ccccaatcgg catctgagta
      541 aaacgataat agtagctatc agggtcgttt tgggctagac tcagcatttt caagaaacgg
      601 tccagatacc aactttcaat atcatccact ccagcatcta catagatgga aaagtcaaag
      661 aagtcagtga tatagagacg atcgttttgt ggattttgaa agacattgat tccctcaaca
      721 attacaaaat cagcagcttt gacactttgt ttctcttcgg gtacgatgtc gtaaacttca
      781 tgagaataga caggaatatc tacatcttgt ccatttttga tgcggtccaa gaagttgaga
      841 agagcttcca tatcatagct ttcaggaaat cctttacgat ttaaaatccc ctgctcaatc
      901 aaggtttgat tgggatagag aaaaccatca gttgtaacca actcaaccgt agcatctgta
      961 aacgtacggg acagtaggat ttgaagtagg cgactggttg tggattttcc aacggcaaca
     1021 ctcccagaaa ccccaataat aaaaggttga gatttacttt cacgttggag gaaaattcct
     1081 tttgaaaagg ctaaatcttc cttagttcgc ttgtaaatct gaatcagatg agccaagggg
     1141 agatagatat ctgtaacgtc ttggagactg atttggtcat taaaactctt gatagattcc
     1201 aattcttctt ctgtcaaagg aggtgttgtc tttcgatgta aagattgcca agtctggcgg
     1261 ctgatttttt caaaatgtaa aaattcgttg gtcatttgtt ttcccctaga tattttgtct
     1321 atcataccat aaaaatctaa aaaaataaag cggtttcttg aatg
//
LOCUS       DAVLYR010000234         1348 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVLYR010000234 DAVLYR010000000
VERSION     DAVLYR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.234"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            18..890
                     /locus_tag="VYO63_001978"
     CDS             18..890
                     /locus_tag="VYO63_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672820.1"
                     /translation="MLPLFVLWLELHVLVNNLLLNLESPLDFVISMSLAFCSLILSKI
                     VLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSPDDLRSRIIWS
                     FILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWEKEDSFES
                     DLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKIEDSHNTLLCT
                     GLVELLLYEISISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNFDFCMNREYHK
                     MKSRKLLLSNKKIV"
     gene            946..1299
                     /locus_tag="VYO63_001979"
     CDS             946..1299
                     /locus_tag="VYO63_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1672821.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKSDCQGICAALAMSPGT
                     FIALAGSAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAGKIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
BASE COUNT          437 a          181 c          252 g          478 t
ORIGIN      
        1 taatcatttt tttattaatg ctccctcttt ttgtcttgtg gctagagttg catgtcctag
       61 tgaataactt actattgaat ctagaatctc cccttgattt tgtgattagt atgagtcttg
      121 ctttctgtag tttgatttta tcaaaaatag ttttagacat actatacgca ttgaaagatt
      181 tgtacaagaa agaagctctt attacgattt ttccttttat ttttataggc aggaagaaag
      241 taaatgtgag gttctcgccc tatttttcat tccatcgtaa aagtttgtcg cctgatgatt
      301 taagatctag gattatatgg agttttattc ttgaaattgc tattatccta gtatttattt
      361 taaaaattcc ttttgcaatc atcatgctta ctacaatatt tttctggact ataatggata
      421 tcaatcatct cgtttttaat aagacggaat ttcttttcaa tcaaaataaa tgggaaaaag
      481 aagattcgtt tgaaagtgat ttgacaaata cgttaaagga taaaatacaa aaatcagatc
      541 tcagttattc tgacttaatg tctcttcagc tgtatgatgc gatgaatcaa tctacctttt
      601 taacggatag tgaactcttt gaggatattc tgagtaaaat tgaagattct cataacaccc
      661 ttctgtgtac aggtcttgtc gagttactgc tgtatgagat ttctattagt aacaataaca
      721 actggtccaa caaggttgat aaaattagga tacacctcat cagaatcaat caattagatt
      781 ttttctatta tacaagttgg ttaaggcaaa attttgattt ctgtatgaat agagagtatc
      841 ataaaatgaa atctcggaaa ttattactga gtaataaaaa gatagtttaa gttcgtccta
      901 tttgtaaaac aaatgaaaca atttcaacta aggaggagaa aacaaatgga acttgtatta
      961 ccaaataatt atgttgtgat tgatgaagaa gaaatgatgt atcttgatgg gggggcttat
     1021 ttaagcaaga gtgattgtca aggaatttgt gcagctttag ctatgagccc aggaactttt
     1081 atagcattag ctggatctgc agttttaact aaaaaactaa taaactatat taagtttgga
     1141 ggtcttggag gctggcttat tggtgcagca gcaggtgtat tggctacagc agcaggaaaa
     1201 atagcttact gtattggata tggtgctctt aatagaggtt gtgatattag cgggaaccct
     1261 tatccttggg atggattcat atctgcgaca gtaagatgaa taatgtagat aagattcgaa
     1321 aaatacatat tatcgtatgt tggatgta
//
LOCUS       DAVLYR010000235         1314 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVLYR010000235 DAVLYR010000000
VERSION     DAVLYR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.235"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..345
                     /locus_tag="VYO63_001980"
     CDS             <1..345
                     /locus_tag="VYO63_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672822.1"
                     /translation="DRLYRMYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYF
                     DKLYKVMNDVERRQLISALISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDE
                     QVECVSLLEKRS"
     gene            613..1179
                     /locus_tag="VYO63_001981"
     CDS             613..1179
                     /locus_tag="VYO63_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672823.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT          472 a          180 c          288 g          374 t
ORIGIN      
        1 gatagactat atcgtatgta tgacaaaata gatgaattag aatcatcact aattgatgcg
       61 aaagcaaaga aacagactat tgaagctgaa aaacttacag gagataacat atataaggtt
      121 ctgatctatt ttgataagct ctataaagtc atgaatgatg tagagcgtag gcagttaatt
      181 tcagctttga tttctgaaat tcaagtttat gaagaaaaac aatctaacgg acaatggcta
      241 aaatccatta cttttaagct accaattata gaagaaaatc tcaacataga tttggacaat
      301 gatgaacaag ttgaatgtgt aagtttatta gagaaacgta gttaatcctc aaaaggttcc
      361 ccaaactttt tgagtcccac tgacgcatca cgctgaggcg gtatcactgc ttgtacgagt
      421 tgaagcatca gcgaagtaaa agtagatgtt cagccaatgc gacgaggtga gaccgaaggt
      481 cgataaggtg tgttctgggt ataggactcg taaagtaagt aggaaatatg atgagatgat
      541 acagagagcc tgaattggtc aggtgacgtg cttatttaca ttagcctgat aatgaaaatg
      601 gagagattat cgatggttat attggaattg taccaaaatg attattctaa agatttagta
      661 gcatttgatt ccatagaaga tgggaaagcc tttgtggctc aaatcccggg atacactctg
      721 gaaacagaag atggttttga agtggagtat tttaatccta aaaacatacc ggattatatg
      781 gaaattattt ttaacggaaa tattgttcct ttatctaaat ttatgttcga tcctgaggaa
      841 aatgtaaaca ttatttggaa agagatttca aatttatccc tcaaaaatga tagagtgatc
      901 gaaggatatt caaaaattga tgcatatgtc gttaacaacc atgaagttaa agcctatgtc
      961 gaaacaagag aaacgaacta tcgtaaagca aaagacttct tagaaagccg cggatatgaa
     1021 atcgacagaa gctttttcgg aagtgaagac ggtgaagcga ttctttacag aaaaaaaggg
     1081 atcgaagttt ggcatttctt gtgtcactta gacccaatgt ttgtagagat tgaagatgta
     1141 gaaggatatg ttaaagaaga agttggagag attcaatagt caaccaaagt tccttgtgag
     1201 tatatacctt tgctagtaaa agagtcaaaa cgttataacg aaggggggga agaaaaccct
     1261 tgagtgtaag tctttttctt gtttagaggg aaagtattag agtagccgct cata
//
LOCUS       DAVLYR010000236         1309 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVLYR010000236 DAVLYR010000000
VERSION     DAVLYR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.236"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(189..1298)
                     /locus_tag="VYO63_001982"
     CDS             complement(189..1298)
                     /locus_tag="VYO63_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1672824.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          493 a          241 c          196 g          379 t
ORIGIN      
        1 gaactttaat agactatgaa aaccagtgga acttaccctg acacagaatt ccactggttt
       61 tttcgatttt tatcagatta acttctacta ttcttagctg tgaaaattag tttaagactt
      121 taacttattt tcagtcttct aatctaaagg ccacttccag taaagaaaaa tcagggttct
      181 cctgactttt acttagctaa ttctctttct cgttctttca ttattttatg attttcatac
      241 aaacgggata agaacttaga aatttctttc aagatagaat aggttggcac tgcgacaatc
      301 attccaacta caccatagat attgcttgac aacaaaagta aaactaaaat cgtgattgga
      361 tgaaccttca taacacttcc tacgattcga ggatataaga tattgccatc tacctgctga
      421 acaacaagca tataaatcac tgcaatcagc agtctatggg gatcagtgaa tatatttgcg
      481 atgatcatag gaatcaaacc aatacttggc cccacataag gaattaaatt ggctacacca
      541 gaaaaaatgg caaaaactaa agcatatttt aaaccaataa tactatagcc aatataagcc
      601 aaacaaccta taatgattgc gtcaatcgaa actccactaa tatagcgagc aatcgtcgca
      661 tttaaattct ttaataagcc tgcaatatgc aagcgatccc tctttagaat cgttctttca
      721 agcatgggca agaatttatg tccatctaat aagaaataaa ccaaaaaaac tggagtcata
      781 atcaaaatca aaacagtact gataagagct gacaagacgc tccccacact atttgatacg
      841 ctatttagga tattttgaag aatatcaaca taggataagt ttaactgctg aattgtagct
      901 tctacatcca aattctggag cgcaggataa ttagataagt ctatgattaa gtcttgtact
      961 cgactataaa tagtttgact agatataatc aaactagata actgattaat caaaataggt
     1021 aagagataga caacacctat gaccattccc cagaccaaag tacacaaggt aattaaaata
     1081 ccaagcaaac gattgagttt acagacttta tttaagaaag taacaatagg gtttgtcaaa
     1141 taataaagaa agccccctaa taaaaatgga atcataattg tattaagcac gctaacaaaa
     1201 gggttaatca aagatcccat ctgtctccat aggtaaaaga tgatggttaa gagtaaaatt
     1261 tctgtggtcc aaaaaaataa tttatttcta cgaaacatga gttacctcc
//
LOCUS       DAVLYR010000237         1306 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVLYR010000237 DAVLYR010000000
VERSION     DAVLYR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.237"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..992
                     /locus_tag="VYO63_001983"
     CDS             <1..992
                     /locus_tag="VYO63_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1672825.1"
                     /translation="VKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSDLNAKAV
                     GVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQEQHIYING
                     KEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPHRVTVTIQN
                     GKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDTFIKPV
                     FKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKSDSTKDVTA
                     TVLDKNNISSKSTTNNLNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
BASE COUNT          526 a          156 c          255 g          369 t
ORIGIN      
        1 atgttaaaga cggtgacatc atggactggg gaatgaaaga ctataaagca aacggatttc
       61 cagataaggt aacagatatg gatggaaatg tttatcttca aactggctat agcgatttga
      121 atgctaaagc agttggagtc cactatcagt ttttatatga taatgttaaa cccgaagtaa
      181 acattgatcc taagggaaat actagtatcg aatatgctga tggaaaatct gtagtcttta
      241 acatcaatga taaaagaaat aatggattcg atggtgagat tcaagaacaa catatttata
      301 taaatggaaa agaatataca tcgtttgatg atattaaaca aataacagac aagacactaa
      361 acattaagat tgttgtaaaa gattttgcaa gaaatacaac cgtaaaagaa ttcattttaa
      421 ataaagatac gggagaggtc agtgaattaa aacctcatag ggtaactgtg accattcaaa
      481 atggaaaaga aatgagttca acgatagtgt cggaagaaga ttttatttta cctgtttata
      541 agggtgaatt agaaaaagga taccaatttg atggttggga aatttctggt ttcgaaggta
      601 aaaaagacgc tggctatgtt attaatctat caaaagatac ctttataaaa cctgtattca
      661 agaaaataga ggagaaaaag gaggaagaaa ataaacctac ttttgatgta tcgaaaaaga
      721 aagataaccc acaagtaaac catagtcaat taaatgaaag tcacagaaaa gaggatttac
      781 aaagagaaga tcattcacaa aaatctgatt caactaagga tgttacagct acagttcttg
      841 ataaaaacaa tatcagtagt aaatcaacta ctaacaatct taataagttg ccaaaaactg
      901 gaacagcaag cggagcccag acactattag ctgccggaat aatgtttata gtaggaattt
      961 ttcttggatt gaagaaaaaa aatcaagatt aagataaaag ctatagaaaa aaatggttta
     1021 tgtactgaga ttagatagtg aggtgatgac atagttttgt gaaaacagcc atttataact
     1081 caattattta gtttacttca ctattgatac tatttggagt tattaatgga cttagtttag
     1141 ataactaatg aattgattga aagggttagt attgacaata ttggtcatat tgattagaaa
     1201 atagagtcta tcaaaattta aaggctaata gaggtgatga gacaatttcg gctctttgtc
     1261 aactgtagtg ggttgaagtc agctaagctc gagaaaggac aaattt
//
LOCUS       DAVLYR010000238         1303 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVLYR010000238 DAVLYR010000000
VERSION     DAVLYR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.238"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..961)
                     /locus_tag="VYO63_001984"
     CDS             complement(<1..961)
                     /locus_tag="VYO63_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672826.1"
                     /translation="MAIISMIVTNLRTLYVYILQMTNRLKDSSVILISDRVLYVLLLF
                     MFIVFGWHEYKVMIWADILGRTFSLMLSFWICKDIVFQPLSKFILDFKESLDNIRVGI
                     NLMLSNIASSMIIGIVRMGIQWNWNIETFGKVSLTLSISNLLMTFINAIGLVIFPLIK
                     RTKTENLPKIYSNLRNALMLVMFAILLFYYPLKFILDIWLPAYKDALVFMALIFPMSV
                     YEGKMALVINTYLKAMRMEKDILKINALVMLTSMVVTLVTTLLLNNLGLTVVSIVILL
                     ALRSIIAELILSKKLKISVKQDIALELLMTIIFISSSWYLSIWI"
BASE COUNT          515 a          221 c          179 g          388 t
ORIGIN      
        1 caatccaaat agagagatac caacttgaag aaataaatat aatcgtcata agtaactcta
       61 aagcaatgtc ttgcttgact gatatcttca gttttttgga taaaattaat tcagctatta
      121 tacttcttaa agcaagtaaa ataactatag atacaactgt cagccccaaa ttatttagta
      181 gtagagtagt cactaatgtc actaccatac tagttaacat aaccaaagca ttaattttga
      241 gaatgtcttt ttccattctc attgctttta aatatgtatt tatcaccaaa gccattttcc
      301 cttcataaac tgacatagga aaaattaggg ccatgaaaac taaggcatcc ttataagcag
      361 gaagccaaat atcaagaata aattttaaag gatagtagaa gagcaagatt gcgaacataa
      421 ccaacatcaa agcatttctt aaattagaat aaattttagg taaattctca gtctttgttc
      481 gctttatcaa aggaaagata actaatccaa tggcattaat aaaagtcatt aataaattag
      541 atatactcaa agttaatgac actttcccga atgtttcaat attccaattc cattgaattc
      601 ccatacgaac aatgcctata atcatgctac tcgcaatgtt agatagcatt aaattgattc
      661 caacacggat attatcaagg gactccttga aatctaagat aaattttgac aaaggctgaa
      721 acacaatatc tttacaaatc cagaaggaaa gcatgagaga aaatgttcga cctagaatat
      781 cagcccaaat catgaccttg tactcatgcc atccaaatac aataaacatg aataaaagga
      841 gtacatataa aacgcgatca ctaattagaa tgactgaact atccttcaag cgatttgtca
      901 tctgcaagat ataaacatag agtgttctta agtttgtgac tatcatgcta atgatagcca
      961 tattataaat atatcttgcg ttttcatccc ttacagttat taggttcaaa gtaaataata
     1021 ccagcgagat tagcattaag aaactagaaa atagaatcat ctgagaataa aactgttttc
     1081 tatctaaatt tgtatattct aagccaccat atttgagata aatcccatca atccaaccca
     1141 agtggaaaaa accgatatag gtcagataaa aaatataaag ttgccagtaa ctgtactcag
     1201 ttacccccat tattttagga aaaatcaaaa caactagtga tgaggttaaa actatgagca
     1261 gatttgaaga aataacataa gaaaaactag ttattatatt ttt
//
LOCUS       DAVLYR010000239         1302 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVLYR010000239 DAVLYR010000000
VERSION     DAVLYR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.239"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            212..552
                     /gene="tnpB"
                     /locus_tag="VYO63_001985"
                     /pseudo
     CDS             212..552
                     /gene="tnpB"
                     /locus_tag="VYO63_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(585..1302)
                     /locus_tag="VYO63_001986"
                     /pseudo
     CDS             complement(585..1302)
                     /locus_tag="VYO63_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          390 a          267 c          236 g          409 t
ORIGIN      
        1 tgtctaactt tttggaaggc agttcattct acacccacta ttatgcgttt ttatcatttt
       61 gaaatctact gctaatcaat gaagaagcaa agatcaaact aggcagctag ttacaagtta
      121 cgtaagcgcg tcataacaag gtatctatcc taattcccca acttccagta tactagataa
      181 aaaataatta tcggaggaaa ggtattgtac tatgataatt caactaagtg atttaggtca
      241 agttcacctt gtttgtagca agatagatat gaggcaggga atagactcat tagcctatgt
      301 agttaaaacc cactttgaat tggatccttt ctccggtcaa gtctttctct tttgtggtgg
      361 acgtaaagac cgctttaaag ccctttactg ggatggtcaa ggattttggc tactatataa
      421 acgctttgag aacggcagac tgacttggcc caatacagaa aaggatgtca aagctctcac
      481 acctgaacaa gtagactggc ttatgacggg cttttctatc actccaaaaa taaatccatc
      541 agaaagtcgt gatttctatt gaaatgagga ctttcttttt atactcatct gctttcaaaa
      601 agcattctat tccatctccg attaacgatg gactttatca cctccttctc cagtccttgt
      661 ataacatctt ggagttgatt catgacatct tccaaagttc gaaaggcttt attcttaaat
      721 ccacgtttac gaatctcttt ccacacttgt tcaatggggt tcatctctgg tgtgtatgga
      781 ggaataaatg caaagccaat attagtcgga atctttaagg tacttgattt atgccataca
      841 gcattgtcca taacgagtaa aagataatca tctggataag cttgtgaaag ctcttctaaa
      901 aaggcgttca tccactcagt attacatcca ccagctatta agaaaaatga ttcgcctgta
      961 taggcatcaa cagctccata acaatagcga aattctcgta tatagtgact atggacatgt
     1021 agacctactc ctattggaga ccaacaagat cccagtttac tgattctacc gaaaccagcc
     1081 tcatcttggt acatcaagca aaccttatga aaacgtctac tggttttaaa gcgctttctt
     1141 ttcttcttga attgagattt tatttttaga cgcgacaatg gtttgagcgt ctgctttctt
     1201 aggatgttct ggacgtggca taatatttcg ccaaccatgg cgcttcaaca gttgatagaa
     1261 ggcatcacgt gtgtaggaac gacctaactc ctttttataa gc
//
LOCUS       DAVLYR010000240         1285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVLYR010000240 DAVLYR010000000
VERSION     DAVLYR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.240"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            130..771
                     /locus_tag="VYO63_001987"
     CDS             130..771
                     /locus_tag="VYO63_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002871528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1672827.1"
                     /translation="MNTSFAHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRFEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKIEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKTLKQELLKKEPSPKFLSKKFQSKQNISMAQLLIKVKK"
     gene            875..1264
                     /locus_tag="VYO63_001988"
     CDS             875..1264
                     /locus_tag="VYO63_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1672828.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT          473 a          195 c          288 g          329 t
ORIGIN      
        1 agaaaagatg ctatactaga agtaggatgt aatggcaaaa tctaatacta ataagcgtta
       61 tgcaatacgt aaactcggaa agatttttgg ttctgcgata atcgggctaa ctttattttt
      121 aggaggaatc tgaatacaag ttttgctcat gctgctgatg ggattcaata tgtcagagat
      181 gatactagag ataaagaaga gggaatagag tatgatgacg ctgacaatgg ggatattatt
      241 gtaaaagtag cgactaaacc taaggtagta accaagaaaa tttcaagtac gcgaattcgt
      301 tttgaaaaag atgaaacaaa agaccgtagt gaaaatcctg ttacaattga cggagaggat
      361 ggctatgtaa ctacgacaag gacctacgat gttaatccag agactggtta tgttaccgaa
      421 caggttactg ttgatagaaa agaagccacg gatacagtta tcaaagttcc agctaaaagc
      481 aagattgaag aagttcttgt tccatttgct actaaatatg aagcagacaa tgacctttct
      541 gcaggacagg agcaagagat tactctagga aagaatggga aaacagttac aacgataact
      601 tataatgtag atggaaagag tggacaagta actgagagta ctttaagtca aaaaaagact
      661 ctcaaacaag agttgttaaa aaaggaacca agccccaagt tcttgtccaa gaaattccaa
      721 tcgaaacaga atatctcgat ggcccaactc ttgataaaag tcaagaagta gaagaagtag
      781 gagaaattgg taaattactc ttactacaat ctatactgta gatgaacgtg atggaataat
      841 tgaagaaact acttctcgtc aaattactaa agagatggta aaaagacgta taaggagagg
      901 gacgagagaa cctgaaaaag ttgttgttcc tgagcaatca tctattcctt cgtatcctgt
      961 atctgttaca tctaaccaag gaacagatgt agcagtagaa ccagctaaag cagttgctcc
     1021 aacaacagac tggaaacaag aaaatggtat gtggtatttt tataatactg atggttccat
     1081 ggcaacaggt tgggtacaag ttaatggttc atggtactac ctcaacagca acggttctat
     1141 gaaagtcaat caatggttcc aagttggtgg taaatggtat tatgtaaatg catcgggtga
     1201 gttagcggtc aatacaagta tagatggcta tagagtcaat gataatggtg aatgggtgcg
     1261 ttaacagatt cctccctgcg acaaa
//
LOCUS       DAVLYR010000241         1270 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVLYR010000241 DAVLYR010000000
VERSION     DAVLYR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.241"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            67..1128
                     /gene="dinB"
                     /locus_tag="VYO63_001989"
     CDS             67..1128
                     /gene="dinB"
                     /locus_tag="VYO63_001989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1672829.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
BASE COUNT          407 a          243 c          278 g          342 t
ORIGIN      
        1 ttgaggttgc agatagagct gacgtggttt gaagagattt tcgaagagta taaaaaggtg
       61 ctaggcatgt tgatttttcc tttgttaaat gatttgtcaa gaaaaatcat ccatattgac
      121 atggatgcct tttttgctgc agtggaaatc agggataatc ctaaactcag aggaaaacct
      181 gtcattattg gaagcgaccc tcggcaaaca ggtggacggg gagtcgtttc tacctgtagt
      241 tatgaggcaa gagcttttgg tgtccattct gccatgagtt ccaaggaagc ttatgaacgt
      301 tgtccccagg ctgtctttat ctcagggaat tatgagaaat acaagtctgt gggactccag
      361 attcgagcta tttttaagcg ctatacagat ttgattgaac ccatgagcat tgacgaagcc
      421 tatttggatg tgacagaaaa taaactcggt atcaagtcag cggtcaaaat tgctcgcctc
      481 attcaaaaag atatctggca agaactccat ctaactgctt ccgcaggcgt ttcttacaac
      541 aaattcttag ctaaaatggc gagtgattat caaaaaccac atggtttgac agtgattcta
      601 cctgaacagg ctgaggattt tctcaaacaa atggatattt ccaaatttca tggagtagga
      661 aaaaagacag tagaacgtct tcatcaaatg ggcgttttta ctggtgctga tttacttgaa
      721 gttcctgagg taaccctaat agaccgtttt ggtagactag gctatgatct gtatcgaaag
      781 gctcgtggca ttcacaactc tccagtcaaa tccaatcgca tccgtaaatc aatcggcaag
      841 gagaaaacct acgggaagat tctccttgct gaggaagata tcaaaaaaga gctgactctt
      901 ctatcagaaa aagtcgctct caatctacat caacaagaaa aagctggaaa aattgtcatt
      961 ttgaaaatcc gctacgagga cttttcaact cttaccaaac gaaaaagtct tgctcaaaaa
     1021 acacaagatg ctagtcagat aagccaaata gccctgcaac tctatgaaga attaagcgag
     1081 aaagaaagag gtgtccgcct attggggatt accctgactg gattttaaag ctcaatgaaa
     1141 atcaaagagc aaactaggaa gctagccgca ggctgctcaa aacactgttt tgaggttgca
     1201 gataaagctg acgcggtttg aagagatttt ctaagagtat aaaaccttga agagttgccc
     1261 cttcaaggtt
//
LOCUS       DAVLYR010000242         1269 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVLYR010000242 DAVLYR010000000
VERSION     DAVLYR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.242"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>1269
                     /locus_tag="VYO63_001990"
     CDS             <1..>1269
                     /locus_tag="VYO63_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1672830.1"
                     /translation="KVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYEDA
                     GKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYGVN
                     LICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNGGFAPYG
                     YKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPLFD
                     AGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDEVW
                     QAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKD
                     FYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVD
                     TSEIEKEIDNYQKELRKSHSTKFKLIEEID"
BASE COUNT          483 a          153 c          288 g          345 t
ORIGIN      
        1 taaaagtata cctctataca cgagtatcta cgtcaataca gatagagggg tattcgttag
       61 aggcacaaaa atcacgaatg aaagcttttg ctatttacaa cgattatgaa attgttggag
      121 agtatgaaga tgccgggaag tctggcaagt ctattgaagg tagaatacag ttcaatcgaa
      181 tgatggaaga tataaaatct ggaaaagatg gagtatcttt tgttcttgtg tttaagttat
      241 caagatttgc aagaaatgcc gctgatgttc tttctacact tcagataatg caggattatg
      301 gagttaatct tatctgcgtt gaagatggca ttgattcatc taaagatgct gggaaactaa
      361 tgatttctgt tttatcagct gtggctgaaa tagaaagaga aaacatccgt attcaaacaa
      421 tggaaggttg cattcaaaaa gcaagggaag gaaaatggaa tggtggcttt gctccgtatg
      481 gatataaact tgaagacggc aagctgttta taaatgagga agaggcagtt gcaataagaa
      541 cgattttcga ccagtatgta aatactacga taggagccaa tgggatatct aaatacttag
      601 agaatcatgg aattagaaaa atcccaagac agaatggtaa gaatcctttg tttgatgcag
      661 gtcttataag aaagatatta aagaatcctg tatataatgg gaaaatagca tttggaagaa
      721 gaactttaga aaaagttcat ggtacaagaa atgaatataa gcaggttgaa caagatgaat
      781 atctaatatc tgaagggata catgaagcta tagtttccga tgaagtttgg caagctgctc
      841 aggttaagct aaaatctcaa gcaaagaaat atgagcatgt gaataaagga aaagatacac
      901 gcacacactt gctttctgga attgtaaaat gcccgatatg tggagtggga atgtttggga
      961 acaagtgtat caagaaaaag aaagatggca caaagtataa agatttttat tactatggtt
     1021 gtaaacatag gcagatgata agaggtcata agtgtacatt cagtaagcaa attagagaag
     1081 aattgttaga tgatgctgtt gcagaggtga ttgtcaagat agtaagtaat ccgaaatttg
     1141 cttctatgat gcaagaaaaa atcaacatga aggtggatac ctctgaaata gaaaaagaaa
     1201 tagataatta ccaaaaggaa ttgagaaaga gtcattctac aaagtttaag ctaattgagg
     1261 aaatagata
//
LOCUS       DAVLYR010000243         1268 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVLYR010000243 DAVLYR010000000
VERSION     DAVLYR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.243"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>1268
                     /locus_tag="VYO63_001991"
     CDS             <1..>1268
                     /locus_tag="VYO63_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1672831.1"
                     /translation="RWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNGIRI
                     INTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRIISQIN
                     KVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQLLEL
                     VYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEGV
                     AQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFID
                     QISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSD
                     IKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYH
                     AKFVAETLSYYCSNNFELEVWTELELSKES"
BASE COUNT          415 a          182 c          208 g          463 t
ORIGIN      
        1 cgttggtttc atcgttctga actagcagag ttattaaatt gtacagaacg tgcagtcaaa
       61 gatgatctat cccatgttaa atctgctttt cctgacttga tttttcattc ttctactaat
      121 ggtatacgca ttattaatac cgatgatagt gatattgaaa tggtttacca tcatttcttt
      181 aaacattcaa ctcatttttc gattttagaa ttcatcttct ttaatgaagg ttgtcaagct
      241 gagagtattt gtaaagaatt ttatatcagt tcatcttcgc tctatcgtat tattagccaa
      301 atcaataaag tgattaaaag gcaatttcaa tttgaagtca gtctgacccc tgttcaaatc
      361 attggaaatg agagagacat tcgttacttt tttgcacaat atttttcaga aaaatattat
      421 ttcctagaat ggccatttga aaatttttca tcagagccac tatctcaatt gttagaattg
      481 gtttataagg aaacaagctt tccaatgaat ttgtcaacgc atagaatgct aaagttgctc
      541 ctagttacga acctatatag aataaagttt ggtcatttta tggaagtaga taaagattct
      601 tttaacgacc aaagtttgga ttttttaatg caggcagaag gaatagaagg tgttgctcag
      661 agttttgaat cagaatacaa tatctcttta gatgaagaag ttgtttgcca attatttgtg
      721 tcttactttc aaaaaatgtt tttcatagat gaaagtctct ttatgaaatg cgtaaaaaag
      781 gatagttatg ttgaaaaatc ttaccatcta ttgagtgatt ttattgatca gatttcagtc
      841 aagtatcaga ttgaaatgga gaataaggat aatctgattt ggcatctgca taataccgca
      901 catctgtatc gtcaagagtt gtttactgag tttattttat ttgatcaaaa agggaataca
      961 atcaggaact ttcaaaatat ttttccaaaa tttgtttcag atataaaaaa agagctttct
     1021 cactatttag agactcttga ggtttgttct agttcaatga tggtaaatca tctatcttat
     1081 accttcatca ctcataccaa acatttggtg attaatttgc tacaaaatca gcctaaacta
     1141 aaggttttag ttatgagtaa ttttgatcag tatcatgcaa aattcgttgc agagacactt
     1201 tcttattact gtagcaataa ttttgaactg gaagtttgga ctgaattaga attatcaaag
     1261 gaatcttt
//
LOCUS       DAVLYR010000244         1254 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVLYR010000244 DAVLYR010000000
VERSION     DAVLYR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.244"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(649..1221)
                     /locus_tag="VYO63_001992"
     CDS             complement(649..1221)
                     /locus_tag="VYO63_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672832.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTTIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT          380 a          220 c          210 g          444 t
ORIGIN      
        1 cttgtaataa gcttgttacc tgataagatt tttggaaaaa ataaaaaaat ttggaaaata
       61 gtttttgcaa tattgacggc agtggcagca ttgtcattta tgtactaagt tattttaaga
      121 atgtagggaa ataaacccta cattcttttt agttttttct gttttctaaa ttctatttat
      181 ccaagcgctt caacatttct tgcttcttcg cttcaagttc tgcacgcttt tcttcgattt
      241 cggcatgttt tttctcgagt tcagaacaac ttgcaccatt gctaaattct tttcgccatc
      301 aggagatagg gtgagtcgac atgtctatta ctcacccaaa gcagtcctac aaagcaggaa
      361 ttttctgtta cttttttgga aatagtaacg tttatacagc tttgacactt cgtatcaaag
      421 cgccaaacac actccgaggg atttacagaa agcagaaaag gaatgatctg gtataagatc
      481 attccttttc tctctttttc tttaagtaat tatatacaat gtacgacgaa gtcgtcattg
      541 caatgctgat ccaccaccta aagggaactt taaacaacat tgataagata aagaatataa
      601 acaacgaaaa tacgttatac ccaattaatt ttattgtata tctcatgatt aaaagttaat
      661 ccttccgttg ttaggaatgg catcattttt atcccataat tgtgctaaat aagtccccgg
      721 tgataataaa ttcatagcgg attctaaagc aacatcattt acaaaccaac tacctagata
      781 tctagaaatt gctgaacgaa tagcactttt tgctgcatgt tttcctttta ctttaattag
      841 atttgcaagg cctgcagtag ttcctcctaa tgctaaagct attgtagtat ctaatagagc
      901 acccatttga ttaactgtaa taccttgcca agctgctcta aatggagagt atgtaggtgg
      961 gattgtataa tcgccttgta attgtcggtt aattacttct ttgatccatt gttgtgagac
     1021 gtctggatga aaagattgga tttcgtttgc aagtgtattg atttgttctt ctgttagaga
     1081 agtgacaggt tgaagttcca tatttgtttc aatttgcgat acttgttcag aagcgtatac
     1141 agctgaaaca cttggaatcg ctgatacaat taacacaatt gacgtcaaaa aaaccgaaat
     1201 aaatttcatt aatttgttca tgagcttttc tcctttttat ttgtatctgc ttac
//
LOCUS       DAVLYR010000245         1234 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVLYR010000245 DAVLYR010000000
VERSION     DAVLYR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.245"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(43..126)
                     /locus_tag="VYO63_001993"
     tRNA            complement(43..126)
                     /locus_tag="VYO63_001993"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Leu,seq:caa)
     gene            complement(136..207)
                     /locus_tag="VYO63_001994"
     tRNA            complement(136..207)
                     /locus_tag="VYO63_001994"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Gln,seq:ttg)
     gene            complement(218..290)
                     /locus_tag="VYO63_001995"
     tRNA            complement(218..290)
                     /locus_tag="VYO63_001995"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:His,seq:gtg)
     gene            complement(305..375)
                     /locus_tag="VYO63_001996"
     tRNA            complement(305..375)
                     /locus_tag="VYO63_001996"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Trp,seq:cca)
     gene            complement(380..460)
                     /locus_tag="VYO63_001997"
     tRNA            complement(380..460)
                     /locus_tag="VYO63_001997"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Tyr,seq:gta)
     gene            complement(473..545)
                     /locus_tag="VYO63_001998"
     tRNA            complement(473..545)
                     /locus_tag="VYO63_001998"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(510..512),aa:Phe,seq:gaa)
     gene            complement(549..622)
                     /locus_tag="VYO63_001999"
     tRNA            complement(549..622)
                     /locus_tag="VYO63_001999"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(586..588),aa:Met,seq:cat)
     gene            complement(633..722)
                     /locus_tag="VYO63_002000"
     tRNA            complement(633..722)
                     /locus_tag="VYO63_002000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(684..686),aa:Ser,seq:tga)
     gene            complement(805..878)
                     /locus_tag="VYO63_002001"
     tRNA            complement(805..878)
                     /locus_tag="VYO63_002001"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(842..844),aa:Ile,seq:gat)
     gene            complement(913..983)
                     /locus_tag="VYO63_002002"
     tRNA            complement(913..983)
                     /locus_tag="VYO63_002002"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Gly,seq:tcc)
     gene            complement(989..1061)
                     /locus_tag="VYO63_002003"
     tRNA            complement(989..1061)
                     /locus_tag="VYO63_002003"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Val,seq:tac)
     gene            complement(1066..1181)
                     /gene="rrf"
                     /locus_tag="VYO63_002004"
     rRNA            complement(1066..1181)
                     /gene="rrf"
                     /locus_tag="VYO63_002004"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          344 a          339 c          279 g          272 t
ORIGIN      
        1 aaaaaaggaa cgaagaataa caacgttcct aacactatac tataccggcg gccggggtcg
       61 aaccggcacg tccttgcgga cactggattt tgagtccagc gcgtctgcca attccgccac
      121 gccggcaaat agtaactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
      181 cttgccttac cgcttggcta taccccaata taaaataggc gagtgatggg gatcgaaccc
      241 acgcatgcca gagccacaat ctggtgtgtt aaccacttca ccacacccgc cataattcta
      301 ttaacacggg cagtaggaat tgaacccaca ctgaaggttt tggagacctt agttctacct
      361 ttaaactatg cccgtaaaat ggaaggggag ggattcgaac ccccgaaccc gaaggagcgg
      421 atttacagtc cgccgcgttt agcctcttcg ctacccttcc aaaatatata aatggcgcga
      481 gacggaatcg aaccgccgac acatggagct tcaatccatt gctctaccaa ctgagctacc
      541 gagccttatt gcgggagcag gatttgaacc tacgaccttc gggttatgag cccgacgagc
      601 taccgagctg ctccatcccg cgttaatata aaaggaggat gtgggattcg aacccacgca
      661 cgcttttaca cgcctgacgg ttttcaagac cgttcccttc agccggactt gggtaatcct
      721 ccaaaatagt ttatacggga acaagcccgt gtcctcttag tgaacagata atattccaat
      781 cccctagtaa tggactaaac actatgggca cgagtggact cgaaccaccg acctcacgct
      841 tatcaggcgt gcgctctaac cacctgagct acgcgcccaa gtcaaaaact tggtacaaag
      901 aacaaagttc aaagcgggtg acgagaatcg aactcgcgac aacagcttgg aaggctgtag
      961 ttttaccact aaactacacc cgctaaaatg ggagttaacg gaatcgaacc gctgaccctc
     1021 tgcttgtaag gcagatgctc tcccagctga gctaaactcc ctagagctaa gcgacttccc
     1081 tatctcacag ggggcaaccc ccaactactt ccggcgttct agggcttaac ttctgtgttc
     1141 ggcatgggta caggtgtatc tcctaggcta tcgtcactta actctgagta atacctactc
     1201 aaaattgaat atctattcaa ttcaagaaaa ccgt
//
LOCUS       DAVLYR010000246         1207 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVLYR010000246 DAVLYR010000000
VERSION     DAVLYR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.246"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..782
                     /locus_tag="VYO63_002005"
     CDS             <1..782
                     /locus_tag="VYO63_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1672833.1"
                     /translation="QLDITIVPLSVMIDNVVYSDADLKEEGKFLQLMQESKNLPKTSQ
                     PPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQGASLSTADVTVVDSSFTDQA
                     LKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGVSTLENLVKGGRISRVTGLLS
                     SLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITSLSERAVAEIGISYSGSDDWA
                     KEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIRYHS"
     gene            889..1164
                     /locus_tag="VYO63_002006"
     CDS             889..1164
                     /locus_tag="VYO63_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1672834.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          389 a          193 c          267 g          358 t
ORIGIN      
        1 agcaattaga tattacaatt gttccattat ctgtaatgat tgataatgtt gtttattctg
       61 atgcggattt gaaagaagaa ggtaaatttc ttcagttgat gcaagaaagt aagaatcttc
      121 cgaaaacaag tcagccacct gtaggtgtct ttgctgagat ttttgaagac ctatgcaaag
      181 atggtggcca gattcttgct attcatatgt cccatgctct ttcgggtacg gtagaagcag
      241 cacgccaagg tgctagccta tctactgcag atgtgactgt tgttgatagt tccttcactg
      301 accaagccct gaaattccaa gttgttgagg ctgcgaagtt agctcaagaa ggtaaagata
      361 tggaggcaat tttatctcat gtagaagagg ttaaaaacca tacagagctt tatattggtg
      421 tttcaacttt ggaaaatctt gtcaaaggtg gacgaattag ccgtgtaact ggcttgttga
      481 gctctcttct caatatccgt gttgtcatgc aaatgaaaga ccatgaattg cagccaatgg
      541 ttaaaggtcg tggaactaaa acatttaaaa aatggttaga tgagttgata acatcgcttt
      601 ctgaacgtgc tgtagcagag attggaattt catattctgg tagtgatgat tgggcaaaag
      661 agatgaaaga aagcttacaa gcttatgttg aaaagccaat ttctgttttg gaaacgggtt
      721 ccattattca aactcacacg ggtgagaacg cttgggctat tttgatacgt taccactcct
      781 aaaaaaataa ataaaatggg aagaaatagc gtttttaact tgacctaaaa gggattttag
      841 gatatgatta tatttgttaa ttagaaatta tttggaggaa tcattaacat ggcaaacaaa
      901 caagatttga tcgctaaagt agcagaagct acagaattaa ctaagaaaga ctcagcagca
      961 gcagttgaag ctgtatttgc agcagtagct gactatcttg cagctggtga aaaagttcag
     1021 ttgatcggtt ttggtaactt tgaagttcgt gagcgcgcag aacgtaaagg tcgcaaccca
     1081 caaactggta aagaaatgac aattgcagct tctaaagtac cagcattcaa agctggtaaa
     1141 gctcttaaag acgctgttaa ataatcagtc tttaaaaagc ctattgtatc aagctttcta
     1201 gcttggt
//
LOCUS       DAVLYR010000247         1207 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVLYR010000247 DAVLYR010000000
VERSION     DAVLYR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.247"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..731
                     /locus_tag="VYO63_002007"
     CDS             <1..731
                     /locus_tag="VYO63_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1672835.1"
                     /translation="FAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYN
                     VNKGSIKIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRA
                     LKLAGCDLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASI
                     DPDNEFELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKD
                     TRYKSLQEMFNSANEWRVSNERVL"
     gene            697..>1207
                     /locus_tag="VYO63_002008"
     CDS             697..>1207
                     /locus_tag="VYO63_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672836.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVII"
BASE COUNT          440 a          170 c          230 g          367 t
ORIGIN      
        1 gctttgctta taatgataaa gctgtcattg aaaatttatc ctttaattta gaagaaggaa
       61 agtcctacgc acttgtcggt tcatctggat caggcaaatc aacagtagca aaacttatat
      121 caggttttta taatgttaat aaaggaagca taaagatagg cgggatagca ataagtgaat
      181 attctgacga agccttaatt aaagccattt cctttgtttt tcaagattca aaattattca
      241 agaagagcat ttatgataat gtagctttag ctaataaaga tgcgacgaaa gatgacgtta
      301 tgagagcctt aaaattagca ggatgcgatt taatattaga caaattccca gaaagagaaa
      361 atacaatcat aggctcaaaa ggtgtttatt tatccggtgg agaaaaacaa agaattgcaa
      421 ttgctagagc aattttaaag gattccaaaa ttattattat ggatgaagca tcagcatcta
      481 ttgacccaga taacgagttt gaattgcaaa aagcttttaa aaatcttatg aaggataaaa
      541 cagttatcat gattgcacac aggctatcta caattaaaga ccttgatgaa attattgtca
      601 tggatagtgg aaaaattata gaaagagggt ctgacaaaga attaatgtca aaagatacaa
      661 ggtataagag cctgcaagag atgtttaaca gtgcgaatga atggagggtt tcaaatgaaa
      721 gagttttata aaaaaagatt tgctcttaca gatggaggag caagaaattt aagtaaagca
      781 acactggctt catttttcgt ttattgtata aacatgcttc ctgccatatt acttatgatt
      841 tttgctcagg aagttttgga aaatatgggc aaaagcaatg gcttttatat agtattctca
      901 gttttgactt tgatagcaat gtatattttg ctttctatcg aatacgataa attatataac
      961 acaacctatc aagaaagtgc agatttaaga ataaggacag cggagaattt atcaaaatta
     1021 cctctatctt acttttctaa acatgacatt tccgacattt cacaaacaat catggctgat
     1081 attgaaggca tagagcatgc aatgagccac tcaataccaa aggtgggcgg catggtactg
     1141 tttttcccat taatatctgt aatgatgcta gcgggcaatg tcaagatggg tttagctgta
     1201 attattc
//
LOCUS       DAVLYR010000248         1146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVLYR010000248 DAVLYR010000000
VERSION     DAVLYR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.248"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(100..816)
                     /locus_tag="VYO63_002009"
     CDS             complement(100..816)
                     /locus_tag="VYO63_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1672837.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(820..1131)
                     /locus_tag="VYO63_002010"
     CDS             complement(820..1131)
                     /locus_tag="VYO63_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672838.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
BASE COUNT          337 a          231 c          167 g          411 t
ORIGIN      
        1 aaaaataggc tccataatat ctatagtgga tttacccact acaaatatta tagagcctgt
       61 agattttcat cttataccat ttccagcaat acaagtagct catagaaaat aagcgagcca
      121 ctcattcatt agactagcga tttctttagg tgcttgagta taaagctcat ggccaaagtt
      181 ttctaaaaaa atagtattaa aatagtctgg caattctttt agggcttcct ctctccatgt
      241 agctccatta ggatagcgag gactaataaa caaggtatct cccacttctc tcttaaaagc
      301 ttgtattttt ctccgtaggc ggagtatcgc ttctatattt tcataattta tagccaactc
      361 atatctatta tactcaacat tccagtgata ggactgtctt acagctttct ccatattttc
      421 tgaccaatgc tttgcttcgg acttctcttt agaaataaga agagctaagt ccgaaacaat
      481 ttgagatttg atataatttt tagtttcctc taactctgta tccaaaggta aaatcttatc
      541 taaatctaga tagccaccat ccaaaagaat cagtttcttt acttcttcaa attccgatgc
      601 gaaataacga gctaaatctc ctccaagaga atggcctatc agacagatag attcttcctc
      661 tacaatctca tttttaaacc atgatttcaa ttctgtttca tctcgaagat gcttttcata
      721 tggatttaga aaatagacct gcgaatctag ttcttgaaaa aaatccttgc tatgataggc
      781 attgcttccc aaaccgctaa taaaatattt tttcattctc tacttaatac tatgcttatt
      841 catcttttgt tcaaagatag ttgtgataat ctgacgcaat tcttcgcgtt ctttttctgg
      901 aatctcacca cttgtttgag ctacagcgta gagttcaggg tattcaattg aaatgcgttt
      961 aatcgtacgt gttgtagcat gttttctgac aaaaaacggg attcgcttaa tcaagtcttg
     1021 tggtactagc gccagaatct tctcagtagt ttctttgtca ctaatattag acattgtaag
     1081 ccttttctta atcatttcct gttctttttc tgtaaaatct tttaattcca ttcgattagt
     1141 cctcct
//
LOCUS       DAVLYR010000249         1107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVLYR010000249 DAVLYR010000000
VERSION     DAVLYR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.249"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..203)
                     /locus_tag="VYO63_002011"
     CDS             complement(<1..203)
                     /locus_tag="VYO63_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672839.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLV"
     gene            complement(217..930)
                     /locus_tag="VYO63_002012"
     CDS             complement(217..930)
                     /locus_tag="VYO63_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1672840.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          361 a          211 c          194 g          341 t
ORIGIN      
        1 ttgactaatg atggagattt ccaagtaaaa attaaatcgc cagagaaatt tttcttatca
       61 aaaaacttaa aaagagattg ataaatacta tttgctaatt gacaagtgtc agatgtaaat
      121 tcataattaa cagcttcatt aaaatcaact tcagacattt caatgtactc ttcaatagta
      181 atattagctt tttttgtagt cattagctaa tctcctttaa aatgtgatct tttcaagaat
      241 atttttatct aaacaatcca gcaagtcttg gtaagaatag acttcgtaag tcggctgggc
      301 ttgtgtgtga ttttcgaggt gatgaggatt ataccagata gtgtcaatcc ccgcattatt
      361 gccaccttga atgtcggcgg ttagagaatc tccaatcatc agcgtctttt ctttactaaa
      421 tccagcaatt tgctggccaa tcttttcata aaagagagca tccggctttt gagtttgcaa
      481 ctgttctgag ataaagactt gattgaaata aggtgctaga ccagattgag ccaaacgtcc
      541 tgtctgaatg gcagtaatgc catttgtcgc agcatacaag tcataatcac gctcaatgag
      601 gctgtccaag agatcatgag cgcccgatag tgtttgtccc tgctgggcga ggtaaaattg
      661 gtaacgctgg gcaagaaaac taccgtcttt ttcctgtcca aaatgagaaa ataaacgaga
      721 aaagcgcgtg ttaaccagct cttgtttact gattttcttc agctccaagt ctttccagag
      781 agccttgttc ataggaacgt aataatcttt ataagcctga atatccgcaa ctccttcttc
      841 ttttagaagt tgggtcaaag ccacatcctc agcagcatca aaatcaagaa gagtgtggtc
      901 gaggtcgaag agtagaaatt tgtagaacaa tttgaggttt tcctttctga aaattcatta
      961 aaaacattat atcataaagc acctcataca attaactaat ttaatcactt aaaaaaaatt
     1021 cgaacacttt ctatacaact gacagctcaa atctttcaga atagaacaat actaactatc
     1081 gagcaccccc gtcttcataa atatatg
//
LOCUS       DAVLYR010000250         1100 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVLYR010000250 DAVLYR010000000
VERSION     DAVLYR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.250"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            29..760
                     /locus_tag="VYO63_002013"
     CDS             29..760
                     /locus_tag="VYO63_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672841.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDISLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLVP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVATLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            778..>1100
                     /locus_tag="VYO63_002014"
     CDS             778..>1100
                     /locus_tag="VYO63_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEV1672842.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVGTSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWTILGASRTQLSLLVSGQ
                     FYLMAV"
BASE COUNT          374 a          168 c          196 g          362 t
ORIGIN      
        1 gatgacactg gaatcaggag gatttattat ggttaataat gtagctgtta aagtctcaaa
       61 cctttcaaaa gaatttttgt tagggcaaga caaaactgtc tctattttga aagatatttc
      121 cttatctgtc aattatgggg aatttgtatc aatacttggt gtcagtggtt ctggaaagtc
      181 tactttgcta agttgcttat caagtttatc tgaaccaaca agtggcgaag ttgttatcaa
      241 tggtgtaaat ccatatacct taaaagaagg gaagcttgct aaatttagaa gacaagatat
      301 tgcaataatt tttcaaaact ataatctggt acctgctcta cctgtactag aaaatgttac
      361 acttcctctg agactatcag gaaagagtgt tgatagcaat aaagtaaaaa aaatgttgga
      421 tagtttgaat tttaaagcag aactatcatc attagttgct accttatcag gtggagaaca
      481 gcaaaaagtg gctattactc gtgcaataat agctgatagt aaaattattt ttgctgatga
      541 gccaacagga gctctggaca gcgtctcaag aaaacttata tttgaaacac ttcgtaattt
      601 agcgagtcaa ggaaaatgtg ttcttatggt cactcatgat attgagttgg cttcaaaaac
      661 tgacagagca ctcattttaa aagacggaaa aatatctcga caaattatta aaccttcagc
      721 tgatgagctc taccaagcac ttgaaagtag taaagattaa tttgaggagg atttattatg
      781 ctaaaattaa tcatttacca atttcagtac tcaaaaagac aatggttagg aacaatacca
      841 ttactatttg tatctagcct gattgttgga acatctttat ttggtattgc tagttcaatc
      901 aagactgcta atattaacgc ttcacaactt tttcaaatgc taataatttt tggcgggact
      961 actctatttt tccttatttc aaataatata agactgctaa tagatatctt taaaaaggat
     1021 taccaattat ggactatctt aggcgcaagc agaactcagc tatctttatt agtatctgga
     1081 cagttttatt taatggcagt
//
LOCUS       DAVLYR010000251         1094 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVLYR010000251 DAVLYR010000000
VERSION     DAVLYR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.251"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            33..446
                     /gene="ndk"
                     /locus_tag="VYO63_002015"
     CDS             33..446
                     /gene="ndk"
                     /locus_tag="VYO63_002015"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1672843.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
BASE COUNT          347 a          188 c          211 g          348 t
ORIGIN      
        1 ataaaagaaa gaagctgaca aaggagacgg gtatggaaca aacattcttt atcatcaaac
       61 cagatggtgt aaaaagaggg ctagtgggtg aagtgttaaa gcgcatcgaa caacgtggat
      121 ttacaatcga aaaattggag tttcgttcac aggtttcaga agagttgatt gaccagcact
      181 atcaggactt ggttggtcag agtttttacc caccgattcg tgaattcatg acttcaggtc
      241 cagttcttgt gggtgtcatt tctggtccca aagtaatcga aacttggcgg accatgatgg
      301 gtgcaactcg tccagaagaa gctttaccag gcactattcg aggtgatttt gcaaaagctg
      361 ctggagaaaa tgagattatc caaaatgttg tacatggttc agattcagaa gagtcagcta
      421 agcgagaaat tgctctttgg ttttaagagt ggattggctc aatcaattgg ttaaaagctc
      481 atttgaatag aaagtatagt caattagttt aagacatgac gcatgatatc aaacttttta
      541 gtttttgata tggtgcgttt tttgattaag tgactattag tttgtctcgc tccgttaggt
      601 gcgagacaaa aaaaacaccc gctatgcggg tgtgcgtcga aggttatacc aaaaaaactc
      661 catacgcgat acaataaagg tgttcaatcc aattgtaaag cgaaaggaga aaaatatggc
      721 acaaaaggtt cttttatcct gttcttgttt caattgacta tatttctata ctttttcttc
      781 ctttctctgt gaaaatcgca agtttttccc accttttctc aatactgaaa caacatcttt
      841 ccagaacttc aataatttct ttttgtgtta aatcattatt taactaaatc tttggaggca
      901 atacgttttc aaagctactt ttttaccata ggttttgagc cttcaacaca gattactaag
      961 ccaaaaatgc tctaaatttt tgaaattcta acaaattcca ctcaacttta tcatcatctt
     1021 tgactttcac aatatcttcc tcaaaataac atggaataaa gatagttttc ttttaaaacg
     1081 gttgtaggag gaaa
//
LOCUS       DAVLYR010000252         1076 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVLYR010000252 DAVLYR010000000
VERSION     DAVLYR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.252"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..>1076)
                     /locus_tag="VYO63_002016"
     CDS             complement(<1..>1076)
                     /locus_tag="VYO63_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV1672844.1"
                     /translation="KIADRLIDIQFWKIALTIIIDLFILYLYRKEIYNLVLSNGYTGS
                     NIQWFFRNATSYEGELTVRTFIRVLIRVIDVSAYIFGYTFINNFLIYRHKRPKDILLL
                     VPLLIFISKTLISGGRQDIIKILIAYVIMMYIQQKRKVGWNRVISHKYIHLGFVGLIA
                     GIPAFYYSLFLAGRSTTRTLFESVSTYLGGSIQHFNQYIENPLDPGEVFGSETLVPIL
                     NILGEMGLVNYRSTIHLEFRTLGVTVGNVYTFFRRPLHDFGLVGMYVFVFAVGAFFAI
                     YYLVLRKKQVGFNLDIHTIIYSYVFYWIFLSSIEQYSFTMISLYTLVFIVLVYFMAIF
                     YWCTDFKRGKLIFKISDSSIKLKE"
BASE COUNT          430 a          188 c          142 g          316 t
ORIGIN      
        1 ttcttttaat ttgatacttg agtcagaaat tttaaaaatc agttttcctc ttttaaaatc
       61 tgtacaccag taaaagatag ccataaagta aaccaacaca ataaatacaa gtgtatatag
      121 actaatcatt gtgaacgagt attgctcgat tgatgataaa aaaatccaat aaaagacata
      181 agaataaata atggtatgaa tatccaaatt aaaaccaacc tgtttctttc tcagaactaa
      241 ataataaata gcaaaaaaag cacctacagc aaagacaaac acatacatac caactagacc
      301 aaaatcatgc aagggtcttc taaaaaaagt ataaacattt cctacagtaa ctcctagtgt
      361 ccgaaattct aaatgaattg tactacgata attaactagg cccatttccc ctaatatatt
      421 taatataggc accaatgttt cactgccaaa aacttcacca ggatctaatg gattttcaat
      481 atactgatta aaatgctgaa ttgagcctcc tagataggtc gaaacactct caaatagcgt
      541 cctagtcgtt gaacgaccgg ctaaaaacaa agagtagtaa aatgctggaa tacctgctat
      601 taaaccaaca aatccaaggt gaatatattt atgagatatg actctattcc atccaacttt
      661 ccgtttttgt tggatataca tcatgattac ataggcaatc agaattttaa taatatcttg
      721 ccggcctcct gatattaaag tttttgaaat aaatattaat aaaggtacta aaagtaatat
      781 gtctttaggg cgtttatggc gatagataag aaaattatta ataaaagtat atccaaaaat
      841 ataagcagat acgtcaataa cacgaatgag aactcgaata aaagttcgca ctgtcaattc
      901 accttcataa ctcgttgcat ttctaaaaaa ccactgaata tttgaccccg tatatccatt
      961 actaagaaca aggttatata tttccttcct gtaaagatac aaaataaaga gatcaattat
     1021 aatagttaga gcaattttcc aaaattggat atcaattaat cgatcagcaa tcttaa
//
LOCUS       DAVLYR010000253         1044 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVLYR010000253 DAVLYR010000000
VERSION     DAVLYR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.253"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(136..>1044)
                     /locus_tag="VYO63_002017"
     CDS             complement(136..>1044)
                     /locus_tag="VYO63_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1672845.1"
                     /translation="NEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTK
                     IAREINSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKR
                     YFKENDINIEFAAEDAKLLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNP
                     YLESSEWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDY
                     RIQYMKEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNG
                     TFNRYKKMSFTWYKGVIESNGESLFK"
BASE COUNT          345 a          207 c          136 g          356 t
ORIGIN      
        1 ctgtaacaaa attaaattaa ttaatccccc tataaaataa agaggtttag aatttgatgt
       61 cttccaaacc tctttatttc atattcaatg agatgttgcc ttaaccgtga taatatctaa
      121 tcatcaataa acaaactatt tgaataagga ttctccattt gattcaatca ctcctttata
      181 ccaagtaaag gacattttct tatatcgatt gaatgtgcca tttccatcat catttcgatc
      241 aacataaatg agaccgtacc tcttagaaag ctgtgcagtg gacatagaaa cgcagtcaat
      301 acatccccaa gacgtatagc ccataatttc cacaccatct tgtagagctt cagctacttg
      361 caataaatgt tctttcatat actgaattct ataatcatct tggactgtta agttattaaa
      421 ttcatctttt attagttgat ctttagcacc taatccgttt tccactataa ataatggaat
      481 ttgataacgg tcataatatc tatttaaaat tatacgtagt ccaattggat caatttgcca
      541 tccccactca gaagactcta aataaggatt tactaagcca ccaataatat tcccttctcc
      601 tgaattatac tgtgttggaa gagcagattg agtcacactc atgtaatagc taaaggataa
      661 aaaatctacg gtataatttt ttaataactt tgcatcttca gctgcaaact ctatgttaat
      721 gtcattttcc ttaaaatatc tttttgcata attcggataa taacctctaa catgcacatc
      781 tgaaaataga taatttagat tctcatactc atgagtcgcc catacatctt ttggatttgg
      841 agtcattgga taagctggca tagctaatac catacacccc accttaaact ctgaattaat
      901 ctcacgagca atttttgtaa ccaaactcga ggcgactaat tcatgatgta tagcttgata
      961 taattcttgt ttcgaaagat tctccttagg tatatctatt cctccactag taaacggtaa
     1021 ttccaaaaca gagtttactt cgtt
//
LOCUS       DAVLYR010000254         1026 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVLYR010000254 DAVLYR010000000
VERSION     DAVLYR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.254"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>1026
                     /locus_tag="VYO63_002018"
     CDS             <1..>1026
                     /locus_tag="VYO63_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1672846.1"
                     /translation="KKFREVDDFTSETGKRIEEYDYKYDDKGNIIAYDDGSALQYETE
                     KLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEIKATKYDSHSKT
                     MIFDLYANINDIVDGLAFAGDMRLFVKDDDRIKAETKIRMPEKNKETKAEYPYVSSYG
                     NVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVTTYNPGK
                     TDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFN
                     IIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSM
                     LFIDTKPV"
BASE COUNT          420 a          122 c          200 g          284 t
ORIGIN      
        1 aagaaattta gagaagttga tgattttaca agtgaaactg gtaagagaat agaggaatac
       61 gattataaat acgatgataa gggaaatatt attgcttatg acgatggtag tgccttacaa
      121 tatgaaactg aaaaacttga cgaaataaaa tcaaaaattt atggtgttct aagcccatct
      181 aaagatggac actttgaaat tcttggaaag ataagcaatg tttctaaaaa tgccaaggta
      241 tattatggaa atagctataa atcgatagaa atcaaagcga ctaagtatga ttcacattca
      301 aaaacgatga tatttgattt atacgctaat attaatgata ttgtagatgg attagctttt
      361 gctggagata tgagattatt tgttaaagat gatgatcgaa taaaagctga aactaagatt
      421 agaatgcctg aaaaaaataa ggaaactaaa gcagaatatc cctatgtatc aagttatggg
      481 aatgtaatcg aattaggaga aggagatctt tcaaagaaca aaccagacaa tttaactaaa
      541 atggaatctg gtaaaatcta ttctgattca gaaaaacaac aatatctgtt aaaggataac
      601 atcattctaa gaaaaggcta tgcactaaaa gtgactacct ataatcctgg aaaaacggat
      661 atgttagaag gaaatggagt ctatagcaag gaagatatag caaaaataca aaaggccaat
      721 cctaatctaa gagtcctttc agaaacaaca atttatgctg atagtagaaa tgttgaagat
      781 ggaagaagta cccaagctgt attaatgtcg gctttggacg gctttaacat tataaggtat
      841 caagtgttta catttaaaat gaacgataaa ggggaagcta tcgataaaga cggaaatctt
      901 gtgacagatt cttctaaact tgtattattt ggtaaggatg ataaagaata cactggagag
      961 gataagtcca atgtagaagc tataaaagaa gatggttcca tgttatttat tgataccaaa
     1021 ccagta
//
LOCUS       DAVLYR010000255         1026 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVLYR010000255 DAVLYR010000000
VERSION     DAVLYR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.255"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            162..>1026
                     /locus_tag="VYO63_002019"
     CDS             162..>1026
                     /locus_tag="VYO63_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1672847.1"
                     /translation="MPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGI
                     NKFLFVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNL
                     PFKQNYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFEN
                     LDKAVTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVI
                     PNPGDLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILT
                     GISKSSVSRELS"
BASE COUNT          371 a          142 c          191 g          322 t
ORIGIN      
        1 tgaaaaaatc agatgttctt gatttaataa agtatcatta tgagggcaga gagacagaat
       61 ttagaaatca atccatagca attgctcgga acttcaataa acatggtgat acacaaatag
      121 cacaatatat tatggggtta atgtctcaaa gtgatagatt catgccacaa atagaaaatc
      181 ctagtgagta tttgactcct gctaaactag atattggtcc tctcccattg cctctttcaa
      241 ttatgaatga tttaaaagga ataataaacg ctgtcaatca tcatattgga ataaataaat
      301 ttttatttgt gggatctcca ggtactggaa aaactgaaag tgtaaagcag gtggctagac
      361 ttattggcaa ggaattactt gtagtagatt tcagtcactt agtagatagt aagctagggc
      421 aaacagtcaa aaacttagca actcttttta atgaaatcaa taatcttcct ttcaagcaaa
      481 attatatcat cctatttgat gaaattgatt ccatagtgtt ggatagggtt aatcagaatg
      541 atttaagaga aatgggacga gtgacttctg cctttttaaa ggagttggat aggctgtcac
      601 cagaaattgt attaattgca acaacaaatc tttttgaaaa tcttgataaa gcagttacga
      661 gaagattcga tgccataatt gactttgacc gttatactga tgaagataaa gtagaagtag
      721 ctactattat tttaaatgag ttattaaagc aatttaagaa tgtagctaga gatttaaaat
      781 tatttaaaaa aattatcaat agtgccaatg taataccgaa tcctggtgat ttgagaaact
      841 caataagaac gtcattggca tttagtgacc catcagatcc gcatgattac caaaaacgcc
      901 tgctaagaag tttgcataat ggtagaaatt tatctatttc taaattatcg aagctaggtt
      961 ttactgttag agaaattgag attttgacag gtatttctaa gagtagtgta tcacgagagt
     1021 taagcg
//
LOCUS       DAVLYR010000256         1020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVLYR010000256 DAVLYR010000000
VERSION     DAVLYR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.256"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            10..>1020
                     /gene="pcpA"
                     /locus_tag="VYO63_002020"
     CDS             10..>1020
                     /gene="pcpA"
                     /locus_tag="VYO63_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1672848.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFY"
BASE COUNT          392 a          149 c          168 g          311 t
ORIGIN      
        1 gaaatttaaa tgaaaaaaac tacaatatta tcattaacta cagctgcggt tattttagca
       61 gcatatgtcc ctaatgaacc aatcctagca gatactccta gttcggaagt aatcaaagag
      121 actaaagttg gaagtattat tcaacaaaat aatatcaaat ataaggttct aactgtagaa
      181 ggtaacatag gaactgttca agtgggtaat ggagttactc ctgtagagtt tgaagctggt
      241 caagatggaa aaccattcac gattcctaca aaaatcacag taggtgataa agtatttacc
      301 gttactgaag tagctagtca agcttttagt tattatccag atgaaacagg tagaattgtc
      361 tactatccta gctctattac tatcccatca agcataaaaa aaatacaaaa aaaaggcttc
      421 catggaagta aagctaaaac tattattttt gacaaaggca gtcagctgga gaaaattgaa
      481 gatagagctt ttgatttttc tgaattagaa gagattgaat tgcctgcatc tctagaatat
      541 attggaacaa gtgcattttc ttttagtcaa aaattgaaaa agctaacctt ttcctcaagt
      601 tcaaaattag aattaatatc acatgaggct tttgctaatt tatcaaattt agagaaacta
      661 acattaccaa aatcggttaa aacattagga agtaatctat ttagactcac tactagctta
      721 aaacatgttg atgttgaaga aggaaatgaa tcgtttgcct cagttgatgg tgttttgttt
      781 tcaaaagata aaactcaatt aatttattat ccaagtcaaa aaaatgacga aagttataaa
      841 acgcctaaag agacaaaaga acttgcatca tattcgttta ataaaaattc ttacttgaaa
      901 aaactcgaat tgaatgaagg tttagaaaaa atcggtactt ttgcatttgc ggatgcgatt
      961 aaacttgaag aaattagctt accaaatagt ttagaaacta ttgaacgttt agccttttac
//
LOCUS       DAVLYR010000257          998 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVLYR010000257 DAVLYR010000000
VERSION     DAVLYR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.257"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>998
                     /locus_tag="VYO63_002021"
     CDS             <1..>998
                     /locus_tag="VYO63_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1672849.1"
                     /translation="NVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGGESYKLY
                     SKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIISDIQDRL
                     GHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDGNDIINL
                     STGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSNQSDLLK
                     LISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPAGSTLSL
                     SELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYKLKDQ"
BASE COUNT          368 a          122 c          200 g          308 t
ORIGIN      
        1 aaaatgtttc tcatctagat aatagaggag gaaatataga gcatgacaaa gataacttag
       61 aatcgtcgat tgtaagaaaa tatgaatggg atatagataa agttactggt ggaggcgaaa
      121 gttataaatt atattctaaa agtaattcta aagtttcaat tgctatttta gattcaggag
      181 tcgatttaca aaatactgga ttactgaaaa atctttcaaa tcactcaaaa aactatgtcc
      241 ccaataaagg atatttagga aaagaggagg gagaggaagg aataatatca gatattcaag
      301 atagattagg tcatggtacg gctgttgtag ctcaaattgt aggggatgac aatattaatg
      361 gagtaaatcc tcacgttaat attaacgtct atagaatatt tggtaagtcg tcagctagtc
      421 cagattggat tgtaaaagca atttttgatg ctgtagatga tggcaatgat attatcaatc
      481 ttagtactgg acaatattta atgattgatg gagaatatga ggacggaaca aatgattttg
      541 aaacattttt gaagtataaa aaggctattg attacgcgaa tcaaaaagga gtaattatag
      601 tagctgcatt agggaatgac tccctaaatg tatcaaatca gtcagattta ttgaaactta
      661 ttagttcacg caaaaaagta agaaaaccag gattagtagt tgatgttcca agttatttct
      721 catctacaat ttcggtcgga ggcatagatc gcttaggtaa tttatcagat tttagcaata
      781 aaggggattc tgatgcaata tatgcgcctg caggctcaac attatctctt tcagaattag
      841 gacttaataa ctttattaat gcagaaaaat ataaagaaga ttggattttt tcggcaacac
      901 taggaggata tacgtatctt tatggaaact catttgctgc tcctaaagtt tctggtgcga
      961 ttgcaatgat tattgataaa tacaaattaa aagatcag
//
LOCUS       DAVLYR010000258          998 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVLYR010000258 DAVLYR010000000
VERSION     DAVLYR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.258"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..729
                     /locus_tag="VYO63_002022"
     CDS             1..729
                     /locus_tag="VYO63_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1672850.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
BASE COUNT          329 a          156 c          180 g          333 t
ORIGIN      
        1 atgagtacaa aatatttatt tatttacaat gagattcgtg aaaagattct ttgtaataaa
       61 tataccatga acgaacaatt gcctgatgaa atgacattag ctaaacagtt tgcctgtagt
      121 cgaatgacga tcaaaaaagc tttagacttg ttagtttctg agggcttaat ttttagaaaa
      181 cgtgggcagg gaacctttgt tctctctcgt ggcagctcaa aaagaaaatt aatcgttcca
      241 gaaagagata tccggggact gacaaaaata tctgaagatg ctcattctac aattgactcg
      301 aggattattc acttcaaatt agaatttgca aatgaatttt tagcagaaaa actacaggtc
      361 gctttgcaga gtccagttta taatatttac cgtctgcgta ttattgacgg taaaccttat
      421 gttctggaac aaacttatat gagtactgat gttattccag gtattactga agatatttta
      481 caaaaatcga tttacaatta cattgaagga aagttaggat tgcatattgc cagtgctaca
      541 aaaatcttgc gagcttcttc tagttcagaa aatgagcaac attacttgca gctccttcca
      601 acggaaccgg tatttgaagt agaacaagtg gcttatttgg ataacggaac tccgtttgag
      661 tactcgatta gtcgtcatcg ctatgattta tttgaattta attcttttgc attacgacat
      721 tcctcctagg agaaaatgtg aaaatgaagc caatctttta cagactctag tttaagaaaa
      781 atttaaaaca gggcaagaag gtcccatcta tgcttaaatg gtttctcttt tctaaataag
      841 atggctttaa aagagtgatc gttttatcca tcatgttgaa aaatatcttc gtatagctta
      901 tagagtaggt actgaaattg ttcacctgat ctacttctta tagttattta gttttaaata
      961 gtgtttcaaa tattcttaca ctgacgagaa gtttttga
//
LOCUS       DAVLYR010000259          987 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVLYR010000259 DAVLYR010000000
VERSION     DAVLYR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.259"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            67..309
                     /locus_tag="VYO63_002023"
     CDS             67..309
                     /locus_tag="VYO63_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1672851.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            311..565
                     /locus_tag="VYO63_002024"
     CDS             311..565
                     /locus_tag="VYO63_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1672852.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHWRDIY"
     gene            612..>987
                     /locus_tag="VYO63_002025"
     CDS             612..>987
                     /locus_tag="VYO63_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV1672853.1"
                     /translation="MNKKWLFADYYDITIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKIS"
BASE COUNT          337 a          126 c          192 g          332 t
ORIGIN      
        1 tttatattga ctgaacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
       61 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
      121 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
      181 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
      241 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
      301 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
      361 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaagaccc
      421 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
      481 taattatcga gttatcgtac aaattgtaga tgatgaatta gttgttgcta ctctagaagt
      541 tggtcattgg agagatattt attgaattaa agctgagtaa taacagtata gaaagtaagg
      601 atagtaaaaa tttgaataaa aaatggttat ttgcagatta ttatgatata acaattattt
      661 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg attgatttgg
      721 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
      781 gttggcgctt ttttattact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt
      841 tattgactat tctcccttta aatgctattt ttacagtgtt tcgattagga cgtatcttcc
      901 gcttagcaag gctgacaaaa ttactgaaac tgactcgttt acttagaata attggactta
      961 caggtaaatt agaaagaaaa attagta
//
LOCUS       DAVLYR010000260          963 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVLYR010000260 DAVLYR010000000
VERSION     DAVLYR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.260"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            21..890
                     /locus_tag="VYO63_002026"
     CDS             21..890
                     /locus_tag="VYO63_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1672854.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
BASE COUNT          244 a          201 c          217 g          301 t
ORIGIN      
        1 ttttaaggag aatctagcaa atggatttca catgggcact gaagtatgcc actgaatttt
       61 tgggaactgc cattttgatc attcttggga atggtgcagt tgccaacgtt gaacttaaag
      121 gtacgaaagg tcaccaaagt ggctggatcg tcatcgctgt tggttatggt atgggggtta
      181 tgatcccagc cttgatgttt ggtaacgtat ctgggaatca catcaaccct gctttcactc
      241 tagggcttgc agttagcggt cttttccctt gggcacaagt ggtaccttac attatcgcgc
      301 aagtcttggg ggctatcttt ggccaagcct tagttgtggc aacataccgt ccattctact
      361 tgaaaactga aaacccaaat aacatcttgg gaactttctc aactatttca agtattgacc
      421 atggtacaaa agaaagtcgc tatgcagcaa ctgtcaatgg tttgattaat gagtttgttg
      481 gttcatttgt tttgttcttt gcagctcttg gtttgactaa aaacttcttt ggtgctgaag
      541 tgcttcaatt catgaaacaa aaggcaatag aagcaggaca aacagttgat ttttctgact
      601 tggctattaa agcacaggtg gctccacaca ctgcttcagg actttctgtg gctcacttgg
      661 cacttggatt cctcgttatg gctttggtaa catcacttgg aggacctaca ggacctgcct
      721 tgaacccagc ccgtgacttg ggaccacgtc tccttcatgc tttccttcca aaatcagttc
      781 ttggtgagca taaaggcgat tcaaaatggt ggtattcttg ggtaccagta gtagcaccta
      841 tcgcagcagc aattgcggca gtagctgtat tcaaattcct ttatctctaa gaaatagctc
      901 ctttaacatt tgagtgagca ccatctataa gtaagagagg atcagactgg ttctctcttt
      961 ttg
//
LOCUS       DAVLYR010000261          949 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVLYR010000261 DAVLYR010000000
VERSION     DAVLYR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.261"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..193)
                     /locus_tag="VYO63_002027"
     CDS             complement(<1..193)
                     /locus_tag="VYO63_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672855.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVF"
     gene            complement(224..538)
                     /locus_tag="VYO63_002028"
     CDS             complement(224..538)
                     /locus_tag="VYO63_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV1672856.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(548..>949)
                     /locus_tag="VYO63_002029"
     CDS             complement(548..>949)
                     /locus_tag="VYO63_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1672857.1"
                     /translation="TESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSSVVFS
                     EKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKK
                     IVSLTENDELQNQLISCNNFEDFINIFEKIK"
BASE COUNT          303 a          178 c          135 g          333 t
ORIGIN      
        1 aaaatactat gagcataatg gggaaaataa taataggcga gggattaata aactgattca
       61 aaagccaata aatattccca aaaaagaaga gtgctattga ataacgtaga agaagatatc
      121 gattaaaaaa agtattagtt agagccatct ctcgacgttg ttgttcaatc ttttgtcgtt
      181 cttttttatc catatcattt cctccttata taacaacaca tatttagtta actttcttat
      241 aaagagctaa catttccttt gctacttcta ataatgtcat agtggtcatt aaatgatctt
      301 gagcatgtac catgataatt tcaattttaa tttccactcc acttgcgtat tcttgcaaga
      361 gtttggtttg tgcatgatgc gcttcaagaa ttatctcatt tgattgattt aatttacttt
      421 ctgcatcatc aaaactacct tctctcattt ttgcaaatgc ttcatgtatt tctgaccttg
      481 catttcccga atgcaggata atttcaaatg ctgcaacctg cagttcctct tgattcatat
      541 gaacctccta ttttatcttc tcaaatatgt taataaaatc ttcaaagtta ttgcaagata
      601 ttagctgatt ttgcaattca tcattctctg tcagagagac tatcttttta gtcacagttg
      661 ccaaaccttc gttcccatat attgatggag atagaagaaa tactagctgg acatgtgaac
      721 tttgattatc ccagagtaac gaatctttac aaattgcaac cgaaaccttt ccctctgtac
      781 caaagggctg aataggatgc ggaactgcaa ttttttcaga aaaaacaact gaacttaatt
      841 cttcgcgctg tttaattcca taaagtaaag attgttcaaa ctcatttgat tcaccaacag
      901 ataaactctc aaccatcttt tcaagtaaat ttaccttgtc tgattcagt
//
LOCUS       DAVLYR010000262          941 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVLYR010000262 DAVLYR010000000
VERSION     DAVLYR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.262"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            197..622
                     /locus_tag="VYO63_002030"
     CDS             197..622
                     /locus_tag="VYO63_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672858.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDNNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
BASE COUNT          343 a          112 c          208 g          278 t
ORIGIN      
        1 gattaggcta tgatacaagt ttatatcgtc ctttgtcaga aagtaaaaag gaaatggtat
       61 taggataata tattaattca actgaacaac ttttagaaaa taacagaatt tcgcaaggga
      121 agtatgagga actgttacta gatgctttca gatatgatat tgtatatggg ctagatgaag
      181 aggggggagt tgtcgtttga ctagtcgtgt atttattgat gcagattgta tttcagtatt
      241 tttatgggtt ggcactgaac atcttttaga aaagctctat tcgggtaaaa ttgttattcc
      301 acaagaggtg tatgatgaaa tcaatatacc tacaattccc catttaaaat ctaggataga
      361 tcagttggta gctaagggtt cagctgagat tgtgagcata gacattggaa ctgaagaata
      421 cgcattatat agagatttaa caagaaatca tgataataac aagattattg gtaagggaga
      481 agcggcttct atttccttag cgaaaaagca taatgggata ttaggaagta ataacctaag
      541 agatgttaaa tcatatgtag aagaattttc tttagaacat atgacaacag gagatatact
      601 gattgaagcg tttaaagcgt aatttattac tgaacaagag ggcaatcata tctggaataa
      661 tatgcttaaa aagagaagga aaattggtgc aaattcattt tcagactatc ttcgtggaag
      721 tattcatcaa aatagacaaa aataaatttg gataaatcga actcactatt caggaggcat
      781 atgagcaatt cgaaaaagaa aagtgtcaaa ttgagcctat aggagtagaa gtgaaatagt
      841 aagtcctgca tagtggatga gagaaaagtt ctccttgaag ttttcctgaa ctatcagtcg
      901 catgtcaaac gatatgtagg gtaatgtgag aggggatagc g
//
LOCUS       DAVLYR010000263          914 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVLYR010000263 DAVLYR010000000
VERSION     DAVLYR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.263"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..178)
                     /locus_tag="VYO63_002031"
     CDS             complement(<1..178)
                     /locus_tag="VYO63_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1672859.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDT"
     gene            complement(191..>914)
                     /locus_tag="VYO63_002032"
     CDS             complement(191..>914)
                     /locus_tag="VYO63_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1672860.1"
                     /translation="KLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKGLLHREHGY
                     AVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAEEICKQKRN
                     VTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHVHQAFVGTD
                     GYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALSQVYEVITD
                     EKLSKQNIATLENAGIMVKVVS"
BASE COUNT          277 a          186 c           99 g          352 t
ORIGIN      
        1 tggtatcaaa attctctatt tcaattttaa caattcctgc attccttgct tctgttaaca
       61 ttctactaat agaggttcta taaattccta attttgctgc tatttgtgac tgatttaagt
      121 tttcaatata atacagataa gcaattttag agagcagttt attcctatct tgattcatac
      181 atttaacctc ttacgaaact accttaacca ttatcccagc attttctaat gtagctatat
      241 tttgtttaga aagtttttcg tctgttatta cttcatagac ttgacttaaa gcaaatcttc
      301 ttactgtacc ccttttatca aatttacttg agtcagttag tacaatgact ttatccgacg
      361 ctgctgaaat atattgaact acctcactgc gcattaaatc ttttcctgta aagcccatct
      421 ctttatcgta accatctgtc ccaacaaaag cttgatgcac atgaaaagtc tgtatcattt
      481 cttttaataa aggtcccaca gtaacctgtg aatccttctg aaactctcca ccaagaacaa
      541 taatacgaca tgaatcataa gctctcacaa aatttgctat aaaaaatgaa tttgttacaa
      601 tcgtaacatt tcttttttgc ttgcaaattt cctcagcaag taaagcacag gtcgatccag
      661 attctatcat tattgtttca ttatctgaca ccaattttac tgcttcctga acaatttttc
      721 tcttaatttc atagttaatt gacaaacgta catttaagtc atctccacta tttaatacag
      781 catatccatg ctctctgtgt aataaacctt ttgactctaa tttatctaaa tcttttctaa
      841 tcgttacttt cgatacattt aatttttccg ataatgtatt aacgtcgatc ttttcatatt
      901 ctgatactaa ttta
//
LOCUS       DAVLYR010000264          905 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVLYR010000264 DAVLYR010000000
VERSION     DAVLYR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.264"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>905
                     /locus_tag="VYO63_002033"
     CDS             <1..>905
                     /locus_tag="VYO63_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1672861.1"
                     /translation="SNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATIAWGN
                     HDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFEAISN
                     SKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGMEKEGDE
                     LIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYLTDLG
                     LVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLGKINAI
                     KNGAVAVIPDNTPLAASCTPTPLSI"
BASE COUNT          356 a          130 c          158 g          261 t
ORIGIN      
        1 cttctaattc tgttaaaaat gaagaaaata cttctaaaga gcatgcgcct gataaaatag
       61 ttttagatca tgctttcggt caaactatat tagataaaaa acctgaaaga gttgcaacta
      121 ttgcttgggg aaatcatgat gtagcattag ctttaggaat agttcctgtt ggattttcaa
      181 aagcaaatta cggtgtaagt gctgataaag gagttttacc atggacagaa gaaaaaatca
      241 aagaactaaa tggtaaagct aacctatttg acgatttgga tggacttaac tttgaagcaa
      301 tatcaaattc taaaccagat gttatcttag caggttattc tggtataact aaagaagatt
      361 atgacactct atcaaaaatt gctcctgtag cagcatacaa atctaaacct tggcaaactt
      421 tatggagaga tatgattaaa attgattcaa aagccttagg tatggaaaaa gaaggtgatg
      481 agttaatcaa aaatactgaa gctcgtatat ccaaagaatt agaaaaacat ccagaaatca
      541 aaggaaaaat caaaggaaaa aaagtattat ttactatgat taatgctgca gatacatcaa
      601 aattctggat ttatactagc aaagatccaa gagcaaatta tttaacagat ttaggtctag
      661 ttttccctga atcattaaaa gaatttgaga gtgaagatag ttttgcaaag gaaatttctg
      721 cagaagaagc aaataagata aatgatgctg atgtaatcat aacttatggt gatgataaaa
      781 ctcttgaagc tttacaaaaa gatcctcttt taggtaaaat aaatgcaatt aaaaatggtg
      841 ccgttgctgt aattccagat aatacaccgt tagcagcctc atgcactcca acaccacttt
      901 caata
//
LOCUS       DAVLYR010000265          902 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVLYR010000265 DAVLYR010000000
VERSION     DAVLYR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.265"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(41..593)
                     /locus_tag="VYO63_002034"
                     /pseudo
     CDS             complement(41..593)
                     /locus_tag="VYO63_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(669..896)
                     /locus_tag="VYO63_002035"
     CDS             complement(669..896)
                     /locus_tag="VYO63_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1672862.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
BASE COUNT          254 a          216 c          171 g          261 t
ORIGIN      
        1 tttagcagtc gatgcgtact gttatagatt caacgcacta taattgatga cgtttagctg
       61 tttccaacaa gctcataata atagctcttg ctttagctcc ttcaaaactt tgagaaaata
      121 accaccctcg cttttttaaa ctgagctcag gctaaaagag tccactggac tcttttactc
      181 cgtcccataa ccaatgattt aatggcgcgt tcagctagat tattggaaag gaccagatgt
      241 ccgtctttca aaatagtctt aaaggtttct tcatacttga ggctgtattc aattgccctt
      301 cctagttttg aacctgctaa aactgactga cggcgacacc aagcaaagaa gtcttccatt
      361 aggggctgga gatgttcttg acgtttctgt agtcgttcat cagctggcaa agcctcccag
      421 tctctttcca aggaaaataa ctgatcacaa taagctaaac ctttagctcc taaggatgat
      481 ttatctgctt gcttgggggt cgcttcaaaa aatttccttc tcacatgcgc ccaacatcca
      541 acaagttcag cttcttccag ttgacgataa gcttctagca gttcgcagtt taccaagctt
      601 agcgtcgcct tgctcctaaa ccttggactg ctatcgcata ggcagaacta gaggactaaa
      661 gtcctaagtt actgccgcaa catatcacaa tgaacatagc cagaataatc tcctaggaat
      721 tcttgtacta ccgaaccact ccgactctga tcatgatggt acagcgtgat tgcttgattc
      781 tcaactttcc cagacaaaaa ggtccaatag taggtcagat ggctatgact ctctagaacc
      841 cgataagaag tttcatccgc atgaagtaag ggctgagtca atagtctctc tcgcaagagg
      901 tt
//
LOCUS       DAVLYR010000266          901 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVLYR010000266 DAVLYR010000000
VERSION     DAVLYR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.266"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            71..868
                     /locus_tag="VYO63_002036"
     CDS             71..868
                     /locus_tag="VYO63_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1672863.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSIFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGSLIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          236 a          156 c          197 g          312 t
ORIGIN      
        1 tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa aaaagattta
       61 ggagtttatt atgatttctt cagaatttat ctcaaagatt gaatttgctt gcaataagaa
      121 agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatat tcgcaggtgc
      181 atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta ataaaattgc
      241 accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg cctacattgt
      301 ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg gtagtttctt
      361 aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct tgttcaacct
      421 tatcggatcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg cgaatctgac
      481 acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa atgaattggt
      541 cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt catttatttt
      601 ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca tgtttgtatt
      661 cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga aattcagtgt
      721 tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact ggggtgtgac
      781 tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg ccttcctcaa
      841 taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg tgcttttttc
      901 a
//
LOCUS       DAVLYR010000267          900 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVLYR010000267 DAVLYR010000000
VERSION     DAVLYR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.267"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..477
                     /gene="rlmH"
                     /locus_tag="VYO63_002037"
     CDS             <1..477
                     /gene="rlmH"
                     /locus_tag="VYO63_002037"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1672864.1"
                     /translation="KIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPD
                     KASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFSTL
                     TFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            521..594
                     /locus_tag="VYO63_002038"
     tRNA            521..594
                     /locus_tag="VYO63_002038"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:555..557,aa:Arg,seq:tct)
     gene            759..899
                     /gene="comC"
                     /locus_tag="VYO63_002039"
     CDS             759..899
                     /gene="comC"
                     /locus_tag="VYO63_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1672865.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIQGGEMRKMNEKSFNIFNFFNFFR
                     RR"
BASE COUNT          320 a          117 c          174 g          289 t
ORIGIN      
        1 aaaattaaag ttgtaacagt tgggaaactg aaagaaaagt atttaaaaga tggtatcgca
       61 gagtattcaa aacgaatttc tagatttgct aagtttgaaa tgattgagtt atcagatgaa
      121 aaaacaccag ataaggccag tgaatcagaa aatcaaaaga ttttagaaat agaaggtcag
      181 agaattttat caaaaattgc tgaccgtgat ttcgttattg tgttagccat tgaagggaaa
      241 actttcttct cagaagaatt tagtaagcag ttagaagaaa cttctataaa aggattttct
      301 actcttactt ttattattgg gggaagttta ggattatcat catctgtaaa aaatagagcc
      361 aatctttctg tcagttttgg tcgcctaacc ttgcctcatc agttaatgag actagttctt
      421 gttgaacaaa tctatcgcgc ttttacgatt cagcagggat tcccctacca taaatagaaa
      481 attgactttt aattgaattt ttggtagaat aattgtgtta ggtctcatag ctcagctgga
      541 tagagcattc gccttctaag cgaacggtcg caggttcgaa tcctgctggg atcaatataa
      601 tagcaaagct gggaattttc ccggcttttt tcttaaaaaa gtacactttg ggagaaaaaa
      661 atgacagttg agagaatttt atctaaaacg aaattccatt ttgtataatg gtttttgtaa
      721 gttagcttac aagaaaaaac attttaggag attttattat gaaaaacaca gttaaattgg
      781 aacagtttgt agctttgaag gaaaaagact tgcaaaatat tcaaggtggg gagatgagga
      841 aaatgaatga aaagtccttt aatatcttta atttctttaa tttctttaga agaagataaa
//
LOCUS       DAVLYR010000268          848 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVLYR010000268 DAVLYR010000000
VERSION     DAVLYR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.268"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>848
                     /locus_tag="VYO63_002040"
     CDS             <1..>848
                     /locus_tag="VYO63_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV1672866.1"
                     /translation="STSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDF
                     DNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVL
                     DRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTF
                     RMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIEDTLKQVHPIFQ
                     TVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALN
                     IKKERT"
BASE COUNT          275 a          176 c          135 g          262 t
ORIGIN      
        1 tcaacttcaa ctgtcattcg caagctcaat gactttcact ttgagcatga tttttctcgg
       61 cttccaaaga ttatgtcttg ggatgagtat gccttcacta agggaaagat gagtttcatt
      121 gcgcaagatt ttgataatct taatattatc actgttcttg aaggcagaac acaagctgtc
      181 atccgaaatc actttcttcg ctacgataga gccgttcgtt gtcaagtgaa aatcattacg
      241 atggatatgt ttagtcctta ctatgacttg gctaaacagc tttttccgtg tgctaaaatc
      301 gttctagatc gtttccatat tatccaacat ctcagccgtg ccatgagtcg ttttcgtgtt
      361 caaattatga atcagtttga acgaaaatct catgaataca aggctatcaa acgttactgg
      421 aaactcatcc aacaggatag tcgtaaattg agcgataaac gtttttatcg ccctactttt
      481 cgcatgcact taacaaataa agaaattctt gacaagattt taagctattc agaagacttg
      541 aaacaccact atcagatcta tcaactctta ctttttcact ttcagaacaa agaccctgag
      601 aaatttttcg gactcattga ggacactctg aagcaggttc atcctatttt tcagactgtc
      661 tttaaaacct ttctaaagaa caaagaaaaa atcgtcaacg ctcttcaatt accttattcc
      721 aacgcaaaat tggaagcgac caataatctc atcaaactta tcaaacgaaa cgcctttgga
      781 tttcggaact ttgaaaactt caaaaaaagg atttttatcg ctctgaacat caaaaaagaa
      841 aggacgaa
//
LOCUS       DAVLYR010000269          841 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVLYR010000269 DAVLYR010000000
VERSION     DAVLYR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.269"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            257..>841
                     /locus_tag="VYO63_002041"
     CDS             257..>841
                     /locus_tag="VYO63_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEV1672867.1"
                     /translation="MLHPIFIIRRSWDGIFHLSEWKRNEEFDFFNKMDVPYLSMSSRI
                     EVTQAINFHKKHSISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTS
                     EDKLNFSSFTMGDNLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVST
                     PMNLSKIDTVPRITWGKVIQENQRYFCTVNLQINH"
BASE COUNT          272 a          124 c          164 g          281 t
ORIGIN      
        1 acctctattc gtagcgccta tagtgggtca gatattaccc cggtatattc attggggtct
       61 ggctctagga ttgtcatgta ctataatgga ggtggtgaca attatattgg ttctggtact
      121 agattagcta tggcgccaca atttggaaat catgtaagaa ttcatacttc aggttcttgg
      181 agtccagatt cttattaact tacttgtcag agtaagcctt aaagatggtt gattgtgggt
      241 gtagcatgaa aaaagaatgc tacaccctat ttttattata aggaggagtt gggatggaat
      301 ttttcattta tcagagtgga aaaggaatga agaatttgac tttttcaata aaatggatgt
      361 tccttattta tctatgagtt cacgaataga agtaacgcag gctatcaatt ttcataaaaa
      421 acattcgata tcactttatg ccataatatc gtggtgtgtt atgagtgcaa taaattctat
      481 tccagaatta ctaatggata ccgacggaaa gattgtctgg caatacaatc aaagaggttg
      541 ttctttcact acccttacat cggaagataa gcttaatttt tcttcattta cgatgggaga
      601 taatttaatt gaatttgttt ctgcgttcaa cataaataaa cagaaggcag aagaagggca
      661 aaaaccaaat attgacaaga ataacattgc ttatctttct tgtgttccat ggattgattt
      721 tttacatgta agcactccta tgaatctttc aaaaattgat acagttcctc gaataacgtg
      781 gggaaaagtt attcaagaga accaaagata tttttgtaca gtgaatttac aaattaatca
      841 t
//
LOCUS       DAVLYR010000270          839 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVLYR010000270 DAVLYR010000000
VERSION     DAVLYR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.270"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(23..790)
                     /locus_tag="VYO63_002042"
     CDS             complement(23..790)
                     /locus_tag="VYO63_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1672868.1"
                     /translation="MQSVTRNPIADPGILGINTGASLSVVIGLSFLGISSSISHISFA
                     IIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSFLILPNNNVLDKFRF
                     WQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEMAVGLGVNVNRIRSL
                     AIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTISSSFIGAELLLICDI
                     IGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
BASE COUNT          302 a          166 c          125 g          246 t
ORIGIN      
        1 atacctataa ttaaattttg cattagttat taacccctct atttttcata gttacataaa
       61 taagtactgg acccccgatt attgcagtaa ttatccctac ttcaatttca cctggtttac
      121 ctaacatacg gccgattata tcacatataa gcaagagctc tgcacctata aaagatgaag
      181 aaatggtcat tgtgcgtata tctttgctta taaataagcc acaaaagtga ggaactataa
      241 gacctacgaa gccaataggt ccaccaattg cagtaatact tgaacataaa agcacacttg
      301 caattattgc aagtgatctt atcctattaa cattaactcc aagaccaaca gccatttcat
      361 cacccatagc taaagcgttt aaatctgatg aaataaatat agctatcaag tgacctaaaa
      421 ttataaaagg tagtagtgta gatatagaag ataatgtagc tgctccaagg ctacctattt
      481 gccaaaatct aaatttgtct aagacgttat tattcggtaa aattaaaaaa cttacaaaac
      541 tgcttaaagc catactaaca caagttcctg ataaggcaag ttttataggg gtaaggcctg
      601 cttttccgct tacagcaatc gcgtatacaa aaattgcact tactaagcca ccaatgattg
      661 caaaacttat atggcttatg cttgatgaaa ttcctaaaaa agaaagacca attactacac
      721 taagacttgc tcctgtgttt ataccgagta tacctggatc agctattggg tttctagtaa
      781 ctgattgcat caatacaccg cttatggcaa gactagaacc tgctaaaatt gcaaaaata
//
LOCUS       DAVLYR010000271          838 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.271, whole genome shotgun sequence.
ACCESSION   DAVLYR010000271 DAVLYR010000000
VERSION     DAVLYR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.271"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..792
                     /locus_tag="VYO63_002043"
     CDS             <1..792
                     /locus_tag="VYO63_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element IS1202 family transposase"
                     /protein_id="HEV1672869.1"
                     /translation="FDKQETLNAYYHVLEQILANHGIPLQMKTDKRTVFTYQASNSKK
                     MEDDTYTQFGYACHQLGILLETTSIPQAKGRVERLNQTLQSRLPIELERNKIHTLEEA
                     NTFLLSYIQTFNEQFGNKTKLSVFEEAPNPSERNLILARLAERVVDSGHHIRFQNRCY
                     IPTEQGKEVYFIRKTKALVLKAFDGDIYLNIADKIYHTKELLDHELYSKNFEQEPEQK
                     KERRKYIPPQTHPWKLTSFKQYLHKNKKDYEEFTSEEIHSPQLQV"
BASE COUNT          299 a          200 c          136 g          203 t
ORIGIN      
        1 tttgacaaac aagagacctt gaatgcctac taccatgtcc tcgaacaaat cttagccaat
       61 cacggcattc cccttcaaat gaaaactgat aagagaacgg tctttaccta tcaagcatcc
      121 aactctaaga aaatggagga cgacacctat acacaatttg gatacgcctg tcaccaacta
      181 gggattctcc ttgagaccac ctctatccct caagctaaag ggagggtcga aagactcaat
      241 cagacactac aatcacgcct gcctatcgaa ctggaacgaa acaagattca tacattggaa
      301 gaagccaata ctttccttct ttcctacatc caaaccttta atgaacagtt tggaaataag
      361 acaaaactct ctgtttttga ggaggctcct aacccctctg aacggaatct cattctagct
      421 agactggcgg agagagtcgt cgatagtgga caccatatcc gattccaaaa ccgttgctat
      481 attcctaccg aacaaggaaa agaagtctat ttcatcagaa agacgaaagc attagttcta
      541 aaagcatttg atggtgacat ctacctcaat atcgctgaca aaatctatca cacaaaggag
      601 ctactagatc atgaactcta ttcgaaaaac tttgaacaag aaccagaaca aaaaaaagaa
      661 agacgcaagt atatccctcc acaaacccat ccgtggaaac tcacatcttt caaacaatac
      721 cttcataaaa acaaaaagga ttatgaagag tttactagtg aggagattca ttctcctcaa
      781 ctacaagtat aatctatttt tcgctaaatg ggaagctttt ccttaaagga agtcactc
//
LOCUS       DAVLYR010000272          815 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.272, whole genome shotgun sequence.
ACCESSION   DAVLYR010000272 DAVLYR010000000
VERSION     DAVLYR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.272"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(100..>815)
                     /locus_tag="VYO63_002044"
     CDS             complement(100..>815)
                     /locus_tag="VYO63_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEV1672870.1"
                     /translation="ILSERISQVAAGVLRNLIDDNMKIGFSWGKSLSNLVDLIHSKSV
                     RNVHFYPLAGGPSHIHAKYHVNTLIYEMSRKFHGECTFMNATIVQENKLLADGILQSR
                     YFENLKNSWKDLDIAVVGIGDFSNKGKHQWLDMLTEDDFKELTKVKTVGEICCRFFDS
                     KGKEVYENLQERTIAISLEDLKNIPQSLAVAYGDTKVSSILSVLRANLVNHLITDKNT
                     ILKVLEEDGDLTFREILGE"
BASE COUNT          282 a          161 c           94 g          278 t
ORIGIN      
        1 gtcaatccaa aaacaacaga taaactattt ttgtttatat aagcacaaat gtgcaatcga
       61 ctaaaaagaa aaaacgataa actgatcagt ctatcatttt cactcaccta gaatctctct
      121 aaaagtcaaa tcaccatctt cttccaaaac ttttaaaatt gtatttttat ctgtaatcaa
      181 atgatttact aaattagcac gcaagacaga aagaatcgaa gatactttcg tatcaccgta
      241 agcaacagct aaactttgag gaatattttt taaatcttcc aaagagatgg ctatcgttct
      301 ttcctgtaag ttttcataga cttctttacc ttttgaatca aaaaatcgac aacaaatttc
      361 tcctacagtt ttaactttgg tcaattcttt aaagtcatcc tctgtaagca tgtctaacca
      421 ttgatgtttc cctttattgc taaaatcacc aattccgact acagctatat ctaaatcttt
      481 ccaactattt ttcaaatttt caaaatatct tgattgcaaa ataccatctg ctaacaattt
      541 attttcttgc acaatcgttg cattcataaa tgtacactct ccatgaaatt ttctagacat
      601 ttcataaatc agtgtattca catggtattt agcgtgtatg tgactaggac cacctgctag
      661 aggatagaag tgaacatttc ggacactttt actgtgaatt aaatctacta aattacttaa
      721 acttttcccc caagaaaagc caattttcat attatcatca attagattcc taaggacacc
      781 tgctgcaact tgagaaattc tttcagataa aattg
//
LOCUS       DAVLYR010000273          811 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVLYR010000273 DAVLYR010000000
VERSION     DAVLYR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.273"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..745)
                     /locus_tag="VYO63_002045"
     CDS             complement(<1..745)
                     /locus_tag="VYO63_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672871.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVH"
BASE COUNT          278 a          160 c          113 g          260 t
ORIGIN      
        1 catgcactaa tttgtctttt tgcatatctt cgtaaagcgc ctctaaagta tctactgcat
       61 aatttccttg agaaatatac atggagtacc acatcattct catgaatgaa acaaagagaa
      121 ctcctgataa aaataaaatc atgataagct caagtaaaat cacctttgcg ctagctaagc
      181 tagtcataaa ataaactata ggaataatta aaattgcaat cagtccaaaa aataaccatt
      241 gatacaaaac ataaggcctt ttacaagata gggaataatc ataagcatac tttgagtaat
      301 cttttatcgc cttataaaag cttttaaaag actctacatt tgctttaaat atttttacaa
      361 cttgcattcc tctcacgtac tcaacagttt cagcacttag tttagatagg gattcttggt
      421 atatcttcat aaattcttgc tcgcccatca ttgcccctaa aattaagccg ccaattatag
      481 taagagcaag caaaattatg ccaactctta tacttactat aaagccaagt gcaagtacaa
      541 gtacgggtgt gattattgcc tgagaactat cgggaatcat gtgtgctacc acctgatgag
      601 tttgtgctgc attatcatct atgatctttc ttatttgacc agatggattt aagtcaaaga
      661 acctaaaact tgctttctct aagccatcaa ttcccctttt tcttaaattt gtttcaagcc
      721 taaatcccaa gatgtgtgaa aacattcctg agacaaaata aaatatcgct ccacttgtta
      781 gtgtaataac agattttaat gctatactct c
//
LOCUS       DAVLYR010000274          802 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.274, whole genome shotgun sequence.
ACCESSION   DAVLYR010000274 DAVLYR010000000
VERSION     DAVLYR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.274"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(700..>802)
                     /locus_tag="VYO63_002046"
     CDS             complement(700..>802)
                     /locus_tag="VYO63_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEV1672872.1"
                     /translation="VLDKEYGGILRYINTELGISSQEIEELKDRYLF"
BASE COUNT          264 a          147 c           97 g          294 t
ORIGIN      
        1 ttttaatatc atttacctac ttccaagatg ctgaaaaata ctaagtcaca ctattatgat
       61 gttattattt ccatttttat tttaacagat gagtcaattc tttcaactac cccctccctt
      121 ataactatat tcatgaagta ttcttgataa taaacctcat ttaacttctc aaagtagtca
      181 gcttctgcaa ctgctattat ctcataacag attgatttgg tttcaataaa tggaagctag
      241 aagccttgtc agctaccctg cacagttggt gttttttcat ttttctcttc aaaaaaattg
      301 gtcggaagtt ataataaaaa ggtgatgtgt ttgtggtgag ttgaattaag agacaaactc
      361 actttgtctt aacttttaaa ttgcctaaca aaaatacgga gattattctc taagctttaa
      421 ctgcaacatt atgtctctac aaccaacgat aagtccagaa atctacccaa ataaccaaac
      481 aacctacatt tgttgaactt tgatgacaag taggaaattt tacatttgat aaacactatt
      541 ttgtacgcat tttgcacata atttaacgtg ctttccatca atttagttaa ggacttacat
      601 agaaaacaat ccgtttttgc ttatgttaaa ttagtagaat tagacaaaaa caaaaccgcc
      661 gttcgcactg ttatatattc atttttagta cttactattc taaaaaagat atctatcttt
      721 caattcctca atttcttgac tacttattcc tagttctgta ttaatatatc taagtattcc
      781 tccatattct ttatcaagga ca
//
LOCUS       DAVLYR010000275          791 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.275, whole genome shotgun sequence.
ACCESSION   DAVLYR010000275 DAVLYR010000000
VERSION     DAVLYR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.275"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..733)
                     /locus_tag="VYO63_002047"
     CDS             complement(<1..733)
                     /locus_tag="VYO63_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672873.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELG"
BASE COUNT          278 a          146 c          109 g          258 t
ORIGIN      
        1 ctcctaattc acctagcgct gtaaaaataa cacctgttag tgctcctatt gcttcaatag
       61 taccaatttt cactattcct tttattgcaa gatagcctgt taaaaaaacg agagatatct
      121 gaaaaaaaat attgagaaag aagctaatag cgcctgctaa cgtttctaca gttgtctttc
      181 tttgtataac catctttaat aaaattcctg cttctttaat tttcttaggc aatacatata
      241 aaagattcaa ggacgctaac acatcaaatc cattcaatat agtctcacta gattttaaaa
      301 aagcttcatt ttggctagtt aaatttagac taacttctcg cattttcgat gcaaagattt
      361 ttggtacaag tagcataatc attaatgaaa acaaggtggc tacagtcaat gaccaatgat
      421 agtgattaag agtcacaact gcaaatatag taccagaaat tccttttatt actaaaaaaa
      481 gttgtttaaa agcctgatca tttaaagtct gaacatcatt atttagccac gaaagatatg
      541 ttcctgatga tttactatga aattcttgat aggtagagtt agagatgtct gtggcaactc
      601 tatttcgaat ctctagatta aactcttgga tcacttcaac ctgataattt ttcactaccc
      661 agtcaaggaa tattatccca caccagacaa tcatttggta gattgacaat ttcaaaaacc
      721 gctctaaatt catcgcaatt aattcattca acaccagagc attaatagtt gctgcataaa
      781 ttagcaataa t
//
LOCUS       DAVLYR010000276          776 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.276, whole genome shotgun sequence.
ACCESSION   DAVLYR010000276 DAVLYR010000000
VERSION     DAVLYR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.276"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..687
                     /locus_tag="VYO63_002048"
     CDS             <1..687
                     /locus_tag="VYO63_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1672874.1"
                     /translation="DELKNKIISGQFSSGDKFYTEAELISMYDVSSITVVRALNDLAK
                     DGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKLVYLEKLGLR
                     GDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFKLDYHIHMND
                     EHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSETYKRSDYYK
                     IKFISCDRDH"
BASE COUNT          260 a          168 c          130 g          218 t
ORIGIN      
        1 gacgaattaa aaaataaaat catctctggg cagtttagca gtggagataa attttacact
       61 gaagcagaac tcatttcaat gtacgatgtc agctctatca cagttgttcg cgccttaaac
      121 gaccttgcca aagatggcta tattgtccgc caacaaggaa aaggaacctt cgtttcacgt
      181 gcgcgcaaac acaagcttgt agagttttca gatattgaac tcttcaatgc taaagatgat
      241 aaggtaactg ttctttcaat tgaacgcgga aacaagcttg tttacttgga aaaattgggt
      301 ctacgcggcg atcaattcta ctacaagatt gaacgtatcc gcgaatcaaa cggtgtcgtt
      361 tatatctacc atacatccta catcccagaa caatatatca acgcaaatta tccaaacctt
      421 gaatactata gttctatcta taatcgtttc aagttagact accacattca catgaatgat
      481 gaacactttg aagaaatcaa cgaaatagtc tttccaactc cagaacatgc ggcttcagtc
      541 ctcggagtcg atgaacaatt ccctactgtc ctacaaacta aaatcaccaa actcgaatca
      601 actggccaag ttttagaata cagtgaaact tacaaacgat ctgattacta caagattaaa
      661 tttatctcat gtgaccgtga tcactaagaa gaaagcctga gctaatcgct tgggcttttt
      721 ctatgcttat tataccacac caaaaaggta gcgcttacct atttttggaa gattct
//
LOCUS       DAVLYR010000277          771 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.277, whole genome shotgun sequence.
ACCESSION   DAVLYR010000277 DAVLYR010000000
VERSION     DAVLYR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.277"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            180..368
                     /locus_tag="VYO63_002049"
     CDS             180..368
                     /locus_tag="VYO63_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672875.1"
                     /translation="MMVGGIFWAILGLSYLEPVTTLALGYQKDISLSLVSGHLAFILF
                     VSCLVVYGTFFLHSEDYV"
     gene            368..>771
                     /locus_tag="VYO63_002050"
     CDS             368..>771
                     /locus_tag="VYO63_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672876.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEV"
BASE COUNT          194 a          103 c          151 g          323 t
ORIGIN      
        1 tttatattgg agattatagt ggtgccttaa aagaattatt tgattctaat cagttgctct
       61 atagtcttgt gacgactctc tggtatggag tttggggcgc tgtgttctct atttttggac
      121 tagctagtgc tttgctagtg aagaaaaaaa taggagctat tttcatccca gttgcctata
      181 tgatggttgg tggtattttt tgggctattt tagggctatc ttacttagaa cctgtgacaa
      241 cattagcttt gggatatcag aaagatatca gtctttcctt agttagtggg catcttgctt
      301 ttattttatt tgttagttgt ttggttgttt atggtacatt ttttctacat tcagaggact
      361 acgtataatg aaacaatttg ttcaatttta taaaaaagat ttcttagcag tattggttta
      421 ttttatatta ctgctatcct gtgttttatc tagtacagta tatttattgc gctgtcgcca
      481 atattcaatc catccaaatg tattagaatg gatcttagtt ttacttcaag atatgacgac
      541 tggagtatat tgctttccgt tcacatatat attgttcttt ttttatttga tgaataacta
      601 ttttaatagg ttggagtgtc gcattcgtct gaaatcaatt aagcacttta ccagttttag
      661 tttcaaatta gcagctctta gtacggggat ttggacggcg actttatttt tattgatttt
      721 tctaattgca tttagtaatg gttttagctt ctctttggag ataaaggagg t
//
LOCUS       DAVLYR010000278          741 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.278, whole genome shotgun sequence.
ACCESSION   DAVLYR010000278 DAVLYR010000000
VERSION     DAVLYR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.278"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>741
                     /locus_tag="VYO63_002051"
     CDS             <1..>741
                     /locus_tag="VYO63_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1672877.1"
                     /translation="LLDIKDPNIQILDIIKKDTHKEIIAKLDYDAPSCPECGNQLKKY
                     DFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIA
                     QKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFI
                     AQDFNNLNIITVLKGRTQAVIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCA
                     KIVLDRFHIVQHLSRAMSRVRVQIMNQF"
BASE COUNT          234 a          158 c          121 g          228 t
ORIGIN      
        1 attactagac attaaagacc ctaatatcca gattttagac atcatcaaga aggatacaca
       61 caaggaaatc atcgccaaac tggactacga cgccccatct tgccctgagt gcggaaacca
      121 attgaagaaa tatgactttc aaaaaccttc taaaattcct tatcttgaaa cgactggtat
      181 gcctactaga attctcctta gaaagcgtcg attcaagtgc tatcactgtt caaaaatgat
      241 ggtcgctgaa acttctatcg tcaagaagaa tcaccaaatc cctcgtatca tcaaccaaaa
      301 gattgctcaa aagttaattg aaaagatttc tatgactgat attgcccatc agctttccat
      361 ctcaacttca actgttattc gtaagctcaa tgactttcac tttaaacatg atttttcttg
      421 tcttcctgag attatgtctt gggatgagta tgcttttaca aaaggaaaga tgagcttcat
      481 tgcgcaagat tttaacaatc tcaatatcat cactgttctt aagggaagaa cacaagctgt
      541 catccgaaat cactttctta aatatgatag agccgtccga tgtcgcgtca aaatcattac
      601 tatggatatg tttagtcctt actatgattt agctagacaa cttttcccgt gtgctaaaat
      661 cgtgttggat cgctttcaca ttgtacaaca tcttagccgt gctatgagtc gtgtgcgtgt
      721 tcaaatcatg aatcagtttc a
//
LOCUS       DAVLYR010000279          728 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.279, whole genome shotgun sequence.
ACCESSION   DAVLYR010000279 DAVLYR010000000
VERSION     DAVLYR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.279"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..663)
                     /locus_tag="VYO63_002052"
     CDS             complement(<1..663)
                     /locus_tag="VYO63_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1672878.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWK"
BASE COUNT          249 a          140 c           88 g          251 t
ORIGIN      
        1 tttccagatt atcaaaatct gagatttata gcacaatatt gatgatattc tatcaatata
       61 atgtttttca tcaagttcct cttgatacat ttttaattct ttagtttttc ccatataact
      121 aaccatacta ctatcactta catatgggaa gtcctcataa tatattactt tataacgcat
      181 aaattcaagc gcccttccaa tactattcac aaaaacatga tcaacatggt caccaagtga
      241 aagcggacaa tatacgacac atttatcgtc taaatgcatt aacagctctt ttatgatatc
      301 attctttaat gtgtcctcat tttttaattc actatagata tgacggtata gaaaattgcc
      361 atttctatct ttcctataga gacattcata gtacgataag tgtctaaaat cacattgtag
      421 acgttcacaa gctaacctgt cttctttctt cctttcttca atcggatatt tcccaaggtt
      481 acacaactta tgaaattgct tagcagaggg ctgtagctgt tggctcaaag ggtaaccaga
      541 aaatatagta ataacaagta caatttctcc ttctgaagtt aattttgaaa tataatcacc
      601 acaggaaaaa attgcgtcat ctaaatgtgg agataaaaag atatacttag tattgttact
      661 cataaccatt ccctctacaa tttatctaaa aactcactaa gtgtctgatt aaattccaca
      721 tcatcaaa
//
LOCUS       DAVLYR010000280          722 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.280, whole genome shotgun sequence.
ACCESSION   DAVLYR010000280 DAVLYR010000000
VERSION     DAVLYR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.280"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            442..>722
                     /locus_tag="VYO63_002053"
     CDS             442..>722
                     /locus_tag="VYO63_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV1672879.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPT"
BASE COUNT          265 a           89 c          135 g          233 t
ORIGIN      
        1 tttgtcccaa tatgaactct actatgtggg acaatcacat agttcaaaga aatgtatcaa
       61 ttctaaagga gttgggacat atatttttat ttgagtctaa aaaaacatat gaggtaggat
      121 tgcgtaaagc aatagattca acatgttcaa tgttacaacc acagtcgtta gtaaaagaac
      181 ttatcaaatt agaaaatatt gtccttgaag agggacatta aaaactactg agaatattaa
      241 ggaggggaaa aaatggaaaa ttcatcaatc gatgtagata tgctgttgga agaattgaca
      301 caagaagcaa tggtcgttgt tgctgttgat aaggactgtt aatttaaact tatggcaata
      361 tatgaaaggt tactggatgt tttaaattat gcaggcagta gccttttatt atatacaaat
      421 ggataaagta aggataatac aatgattaat aaaaaaatac aacaagttgt tttggaatca
      481 ttacagaatt ttttgaatgg gaacttcatt tcgccttgtg tagtctatga ttttggcttg
      541 ctggaaactg tacttgatga atttaaaaat caaattcctg taacattcaa ttaccaactt
      601 ttttatgccg ttaaagcaaa ttcaaatgag aagatacttg aattcttagt agataaaatt
      661 gatggagttg atgtggcgtc attatctgaa ttagatgtgg ctaaaaaatt tttcccacca
      721 ac
//
LOCUS       DAVLYR010000281          717 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.281, whole genome shotgun sequence.
ACCESSION   DAVLYR010000281 DAVLYR010000000
VERSION     DAVLYR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.281"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>717
                     /locus_tag="VYO63_002054"
     CDS             <1..>717
                     /locus_tag="VYO63_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1672880.1"
                     /translation="EVSINKYHAQVGAYLQGEEAVLYQSFSEIKELTSEDIVVDYIME
                     TRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGKFIKPKAGSKVFT
                     GRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVRPYTGDYHAQFDA
                     SVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLSPLKSINFHRARW
                     KEMVKPYFEKNEIFKIQQDVV"
BASE COUNT          232 a           90 c          158 g          237 t
ORIGIN      
        1 tgaggtttca attaataaat accatgctca agtaggtgcc tacttacaag gagaagaagc
       61 tgttttatat caatcttttt ctgaaataaa agagttaaca agtgaagata tagttgtaga
      121 ttatataatg gagactagag cattactaaa aatgatgggc ttaaacgttc cggttcatga
      181 ctatcctatt gagcttaaag agttttatgg tcgaaagatt tatgctggta ttttaggaga
      241 gattgtgaat atacctgata attggggaaa atttattaag cctaaagctg gttcaaaagt
      301 ctttactgga agagttgtta atggaaccca tgatttaata ggtattggtc tacctttcga
      361 ctatcctata tggattagtg aggttgtaga attcatagct gaatggcgtt gttttgtgtt
      421 agatggtcgc gtattagatg ttcgacccta tacaggtgat tatcatgcac aatttgatgc
      481 aagtgtaatt gatgaagcga tatcatgttg gaaagatgcg ccaatagctt atggactaga
      541 tatcggtgtt actcgcgatg gcagaacact tgttgttgaa gtaaatgatg gttatgcatt
      601 gggaaattat ggcctatctc ctttaaaatc aatcaatttt catagagcta ggtggaaaga
      661 aatggtaaaa ccctattttg aaaaaaatga aatttttaaa atccaacagg atgttgt
//
LOCUS       DAVLYR010000282          716 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.282, whole genome shotgun sequence.
ACCESSION   DAVLYR010000282 DAVLYR010000000
VERSION     DAVLYR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.282"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>716
                     /locus_tag="VYO63_002055"
     CDS             <1..>716
                     /locus_tag="VYO63_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1672881.1"
                     /translation="VEYNLKKKFLTYFDFWTYSMGDAFICVSETMKKRLESIIKNKKI
                     VAINNGINVSNSDLDVVGVQDFKKEFCIPNNKKIICYVGRLDPEKRPDRFLEFAEKLF
                     LVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSDT
                     EGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDSE
                     YLKYIGENSKSKIRKHFSVQ"
BASE COUNT          252 a           65 c          152 g          247 t
ORIGIN      
        1 gggttgaata taacttaaaa aagaaattct tgacttattt tgatttttgg acatattcta
       61 tgggagatgc ttttatttgt gtatctgaaa ctatgaaaaa aagattggag agtataataa
      121 aaaataagaa gatagttgca ataaataacg gaattaatgt atcaaatagc gatttagatg
      181 tagtaggtgt tcaagacttt aaaaaagaat tttgtattcc aaataacaaa aaaatcattt
      241 gttatgttgg aaggttggat ccagaaaaaa ggccggatag attccttgaa tttgcagaaa
      301 aattattttt agtcagagaa gatgtgattt ttattatggc tggaaatggt agtatgtggg
      361 ctgctttgaa agaaaaaatt tgccatttga aatgtagaga taatttcagg ttacttggtg
      421 aaatctatcc agctactata gtttaccaaa tatcggattt attgtatatt ccttctgata
      481 cagaaggaat acctatgtgt gttttagagt caatgtcaca aggaactccg gttttagcta
      541 gtaatgttgg cgggttaagt gaaattattg aacatagggt tgatggattt ttatttgaga
      601 aggaagatgt tgagggagtg tgtgcttgtg ctaatttttt actcaatgat tctgagtatt
      661 tgaaatatat aggtgagaat agtaaatcaa aaataagaaa acatttttct gtgcaa
//
LOCUS       DAVLYR010000283          707 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.283, whole genome shotgun sequence.
ACCESSION   DAVLYR010000283 DAVLYR010000000
VERSION     DAVLYR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.283"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            172..426
                     /gene="blpM"
                     /locus_tag="VYO63_002056"
     CDS             172..426
                     /gene="blpM"
                     /locus_tag="VYO63_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1672882.1"
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            442..645
                     /gene="blpN"
                     /locus_tag="VYO63_002057"
     CDS             442..645
                     /gene="blpN"
                     /locus_tag="VYO63_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1672883.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          235 a           81 c          178 g          213 t
ORIGIN      
        1 tagaaattat ctttttttaa aaaatttaga ggtggtttca aatgaaaagc taattcaaga
       61 cgtttcgatg actattcaag atctggatga aaaattttaa aaaacgatga tatactaaac
      121 ttgtcaaagt tacaacaaga caaaaattaa aaataaaaaa gagtatttgt catgaataca
      181 aaaatgatgg aacaatttca tgaaatggat ataactatgt tatctagtat tgaaggaggc
      241 aagaataatt ggcaaactaa tgtcttagaa ggtggtggtg ctgcttttgg tggctggggg
      301 ctagggacag ctatttgtgc tgcgagtggt gttggagcac catttatggg agcatgtgga
      361 tacataggag ctaaatttgg tgtggatctt tgggcaggag taacaggggc aacgggtgga
      421 ttttaataaa aggagacttg tatgaatact tattgtaata taaatgaaac aatgctgagt
      481 gaggtttatg gaggtaattc cggaggagca gctgtagttg ctgctttagg ttgtgcagcg
      541 ggtggagtga aatatgggaa aattctagga ccatggggcg ctgcaatagg aggaattgga
      601 ggagcagtgg tttgtggata tttagcctat accgctacat cataaaaatg aaagctaaat
      661 tttatgaagg ctatattctt tattattctc ttcgcttttc aaaccta
//
LOCUS       DAVLYR010000284          689 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.284, whole genome shotgun sequence.
ACCESSION   DAVLYR010000284 DAVLYR010000000
VERSION     DAVLYR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.284"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>689
                     /locus_tag="VYO63_002058"
     CDS             <1..>689
                     /locus_tag="VYO63_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1672884.1"
                     /translation="NSNAQLARQFIIGLPKELSLSENKNLVERYIKENLTSQGMIVDY
                     AIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKEYILDEKGEKVLNKNGKPKTRK
                     VELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVDHRSFKRQNSDYLPTIHLGSAA
                     SAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLKGWIGNLLDNLSTAYEKFKDIE
                     RDKVIDNPKLF"
BASE COUNT          332 a           82 c          122 g          153 t
ORIGIN      
        1 aaaattctaa tgcacaacta gcaagacaat tcataatagg actaccaaaa gagttatctt
       61 taagtgaaaa taaaaacctg gttgaaagat atataaaaga aaatttaaca tcacaaggaa
      121 tgatagtaga ttatgcaatt catgatgaga gtcaagataa aaatggaaat atccattgtc
      181 atataatgac cataatgcga ccaataaacg aaaaaggaga gttcttagcc aagtcaaaaa
      241 aagaatatat cctagatgaa aaaggagaaa aagtattaaa caaaaatgga aaaccaaaga
      301 caagaaaagt agaactaaca acctggaacg atacaggcaa tgtagaacaa tggagagaaa
      361 atttttcaga tctttgtaac aaatatttag aaagagcagg agcagaaaaa agagtagacc
      421 ataggagttt taaaagacaa aattcagatt atctaccgac aatccattta ggatcagcag
      481 caagtgcaat ggaaagaaag ggaatagaaa ctgacaaggg aaactataat agagaaataa
      541 gaaaatataa tcaacttgta aaaacaataa aagaagagat aaaaacatta aagggttgga
      601 taggaaattt attagataac ttatctactg cttatgaaaa atttaaggat atcgaaagag
      661 ataaggtaat agacaaccct aaacttttt
//
LOCUS       DAVLYR010000285          686 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.285, whole genome shotgun sequence.
ACCESSION   DAVLYR010000285 DAVLYR010000000
VERSION     DAVLYR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.285"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            262..486
                     /gene="pneA1"
                     /locus_tag="VYO63_002059"
     CDS             262..486
                     /gene="pneA1"
                     /locus_tag="VYO63_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEV1672885.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            493..681
                     /gene="pneA2"
                     /locus_tag="VYO63_002060"
     CDS             493..681
                     /gene="pneA2"
                     /locus_tag="VYO63_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEV1672886.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT          234 a           89 c          143 g          220 t
ORIGIN      
        1 cgtggaatta aaaaattagt tacatttgca ttgctaggtg tttttatgtt tagtaataca
       61 attccttacc aacagtttat tcagaagaat aaacaattgg agattcgagt gcaatcgcaa
      121 aagaagtcca atggtcttga tgttgggaag gcggattaaa acccgatatt ttgcacgaga
      181 atgagagaat tataatttat ctgaaaaaac gaaaaatata gagcaactta tttaactttt
      241 tataaggagg agttattact tatgaccaac tttaattcaa acgaaaaatt ttgtggaaaa
      301 agtttaaaat cattgtctgc tgatgaaatg agtttaattt atggtgcatc tgatggtgca
      361 gagccacgtt ggacgcctac tcctattatt ttgaaatcag ctgcagctag ctcgaaagta
      421 tgcattagtg cagctgtttc tggtattggg gggctagtta gctacaataa tgattgtttg
      481 gggtaaatta aaatgaaaaa tgattttgta atcggaaaga gtttgaaaga attatctctt
      541 gaagaaatgc aattggttta tggtggcaca gatggtgctg accctagatc aacaattata
      601 tgttctgcta cattaagttt tattgcatca tatttggggt cagctcagac tcgttgtggc
      661 aaagacaaca agaaaaaata aaatat
//
LOCUS       DAVLYR010000286          673 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.286, whole genome shotgun sequence.
ACCESSION   DAVLYR010000286 DAVLYR010000000
VERSION     DAVLYR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.286"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>673
                     /locus_tag="VYO63_002061"
     CDS             <1..>673
                     /locus_tag="VYO63_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1672887.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
BASE COUNT          311 a           88 c          126 g          148 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata
      481 aaaatctaaa agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat
      541 acatggaaag cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg
      601 aagaagataa agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag
      661 gagaatcttt ata
//
LOCUS       DAVLYR010000287          658 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.287, whole genome shotgun sequence.
ACCESSION   DAVLYR010000287 DAVLYR010000000
VERSION     DAVLYR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.287"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..658)
                     /locus_tag="VYO63_002062"
                     /pseudo
     CDS             complement(1..658)
                     /locus_tag="VYO63_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          210 a          125 c           98 g          225 t
ORIGIN      
        1 catttatata ttctaagtaa gaatcccaag cctcatcaaa attatcttgt cctccaatca
       61 attgggtttc ttttgctttc tgacaaaaat ctttaaaaat accgtcctgt agcctaaact
      121 caatagctcc atccccattt gatactggtc ttaaaccttc tacaaaatcc gtataatcat
      181 atgatgggtg aaattgtaca aatccgattt ggtcttcgtt gccatccgtt aattctttgg
      241 caatttcttt agcaagataa gtctttcctg tgccaggagc accacgaagg ataaggtttg
      301 gagactttaa tagagattga gttaattgat gacgaaaatt gtttgatgaa tccattgtac
      361 tattgtcttc cttattttcc ataaaaagct tagaatactc aaaaagcata gttcctaatt
      421 tcgatggctc caactcagga taagttttgg caccagttca attattttat gattttgcag
      481 ataaggtcca ccttgatttg tctgagaaac aaagtggttc caaaattctt tctgactatt
      541 aatatttaca ccaaagaaag aatctccctc agaatagata caaagtaatt ttgtcaccat
      601 agcagaacga ccaataaatt catttgttga tcttttcata tcaaaaatag gattttca
//
LOCUS       DAVLYR010000288          657 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.288, whole genome shotgun sequence.
ACCESSION   DAVLYR010000288 DAVLYR010000000
VERSION     DAVLYR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.288"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            178..549
                     /locus_tag="VYO63_002063"
     CDS             178..549
                     /locus_tag="VYO63_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEV1672888.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
BASE COUNT          211 a           83 c          148 g          215 t
ORIGIN      
        1 gaatcagtga ttagctgtta tttgaattaa gtaaactgtt aagcttcaag cagtaactgg
       61 ttttaaatat ttaaaataag ataaaggaag gaagtctaat ttaggtaact aaacaaagcc
      121 tgcgatcact tgttaggaaa ccatcctaag catgcttaat aaaaaaggag aaaataaatg
      181 gaacttgtat taccaaataa ttatgttgtg attgatgaag aagagatgat gtaccttgat
      241 gggggggcta tatatatacc caggtgggca attacaggag ccattactgg tgcagcatat
      301 gcagcattag cagcagcagg aggtggaggc cttcaactag ttcttgcatc ttatggatta
      361 cgctccgcac tggtagctgg gattgttaaa ggtttaggag tattaggaat tcatattgga
      421 aatgcttttg caaatactgt tattagaagt attgcatctg ctggaattgg tgctggagct
      481 gattggattt ttaccaatat tattgatggc tgggatgggc gacgtgataa tcaattgaga
      541 ataggttaat aatcttatgt tgattgtttt aatttcagtt attgttgcat tcctgtatca
      601 taaaattaca gagtatgtca acaacaggaa aattgatatt gttatttttc tatcatt
//
LOCUS       DAVLYR010000289          646 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.289, whole genome shotgun sequence.
ACCESSION   DAVLYR010000289 DAVLYR010000000
VERSION     DAVLYR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.289"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..577
                     /locus_tag="VYO63_002064"
     CDS             <1..577
                     /locus_tag="VYO63_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1672889.1"
                     /translation="DLAKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDL
                     EIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQ
                     IELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLV
                     KDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          237 a          122 c          105 g          182 t
ORIGIN      
        1 agatttagca aaacacatag ctatattacc tcaatctcca ataatccctg aatcaataac
       61 agtagctgat cttgtaagcc gtggtcgttt cccctacaga aagcctttta agagtcttgg
      121 aaaagatgac cttgaaataa taaacagatc aatggttaag gccaatgttg aagatctagc
      181 aaataaccta gttgaagaac tttctggggg tcaaaggcaa agagtatgga tagctctagc
      241 cctagcccaa gatacaagta tcctactttt agatgagcca actacttact tggatatctc
      301 atatcaaata gaactattag acctcttgac tgatctaaac caaaaatata agacaaccat
      361 ttgcatgatt ttgcacgata taaatctaac agcaagatac gctgattacc tatttgcaat
      421 taaagaaggt aaacttgttg cagagggaaa gcctgaagat atactaaatg ataaactagt
      481 taaagatatc tttaatcttg aagcaaaaat tatacgtgac cctatttcca attcgcctct
      541 aatgattcct attggcaagc accatgttag ctcttaataa ttgaatatcg gaattcattt
      601 aaaagtcgca aattttgaac tgaccctcaa aagttaggcc aaaaaa
//
LOCUS       DAVLYR010000290          633 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.290, whole genome shotgun sequence.
ACCESSION   DAVLYR010000290 DAVLYR010000000
VERSION     DAVLYR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.290"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(66..>633)
                     /locus_tag="VYO63_002065"
     CDS             complement(66..>633)
                     /locus_tag="VYO63_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV1672890.1"
                     /translation="DDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFMENRLKE
                     FFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVITLDTSIY
                     TNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSIVFFPVG
                     AYIKSNHSNLHRNDFMREVYLWTKNLTY"
BASE COUNT          213 a          107 c           74 g          239 t
ORIGIN      
        1 aaattcttta tattgattat ccagaatttg agtagtttta tttcctaaat attccttaac
       61 tttatctaat atgtcaagtt ttttgtccac aaatatacct cccgcataaa atcattacga
      121 tgtaaatttg aatgattgct ttttatataa gctcctacag gaaaaaacac tatactgcta
      181 ttttgagtta accttggaag cgaaactttc attttatact tgtcatgttc ataacaggta
      241 ttaccataga gaatagtact ataacgctct ttactacttg aatttaacgt tgtaataata
      301 tgcggaacaa accataatgt attggtgtat attgatgtgt ctaacgtgat aacctgctgc
      361 tcattcacct cactaacatt aactgggcta gtaatcatat aggcagaaaa tgaagtaatt
      421 gcactacctg gttctattat tgtagtcaca gtactatcaa tatcgtactt tttatagatt
      481 tccataagtg atagaaaaaa ttctttcaaa cgattttcca taaataattt cataaaaccg
      541 cctccaagat ttatttctct aacctctcta agtttattaa tttcttgtaa cttaaataat
      601 attttatcta acttaacaat aaatttatca tct
//
LOCUS       DAVLYR010000291          631 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.291, whole genome shotgun sequence.
ACCESSION   DAVLYR010000291 DAVLYR010000000
VERSION     DAVLYR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.291"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>631
                     /locus_tag="VYO63_002066"
     CDS             <1..>631
                     /locus_tag="VYO63_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672891.1"
                     /translation="DYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYHKVKD
                     QYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQGRNRK
                     LDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISYYLVDP
                     DQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEK"
BASE COUNT          247 a           83 c          133 g          168 t
ORIGIN      
        1 gattacccat ggcaggtttc ctatattcct gtaaaaattg ataacaccgc ccctaagatt
       61 gtttcggttg atttttcaaa tcctgaaaaa attaagttga ttacaaagga tacttatcat
      121 aaggtaaaag atcagtataa gaatgaaacg ctatttgcga gagaccaaaa agaacatcct
      181 gaaaaatttg acgagattgc gaacgaagtt tggtatgctg gcgccgctct tgttaatgaa
      241 gatggagagg ttgaaaaaaa tcttgaagta acttacgcag gtgagggtca aggaagaaat
      301 agaaaacttg acaaagacgg aaataccatt tatgaaatta aaggtgcggg agatttaagg
      361 ggaaaaatca ttgaagtcat tgcattagat ggttctagca atttcacaaa gattcataga
      421 attaaatttg ctaatcaggc tgatgaaaag gggatgattt cctattatct agtagatcct
      481 gatcaagatt catctaaata tcaaaagctt ggcgagattg cagaatctaa atttaaaaat
      541 ttaggaaatg gaaaagaggg tagtctaaaa aaagatacaa ctggggtaga acatcatcat
      601 caagaaaatg aagagtctat taaagaaaaa t
//
LOCUS       DAVLYR010000292          629 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.292, whole genome shotgun sequence.
ACCESSION   DAVLYR010000292 DAVLYR010000000
VERSION     DAVLYR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.292"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..275)
                     /locus_tag="VYO63_002067"
     CDS             complement(<1..275)
                     /locus_tag="VYO63_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component"
                     /protein_id="HEV1672892.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQLASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAE"
     gene            complement(297..503)
                     /locus_tag="VYO63_002068"
     CDS             complement(297..503)
                     /locus_tag="VYO63_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672893.1"
                     /translation="MDLSNKASNLRKKLGADGEAPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(490..>629)
                     /locus_tag="VYO63_002069"
     CDS             complement(490..>629)
                     /locus_tag="VYO63_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1672894.1"
                     /translation="IKELRISSNLTQDKIAEYLSLYLSLNQSMIAKMEKGERNITNGF
                     K"
BASE COUNT          198 a          134 c           74 g          223 t
ORIGIN      
        1 tcttctgcat caaggtatcc atcattcctc aatctatata acatagcttt atgactgata
       61 ccataaaact gacccaattt tataatatct tctacttcaa gatgagttct attggcattt
      121 tctctgattt cctcaaccat cctatacagt gaagatggga aaattaaaaa ataagaagca
      181 agctgatccg cttttctttc agtttcatct ccttcaccaa tcaagataag actgactgaa
      241 ctcttcttca cctcatcata ataaagatga tacagttcat gtgctaaaga aaatctttac
      301 cttcctaatg acatgtctga attgactgca atgagactga actgagttcc tttataacag
      361 actccgctaa tattctttcc gagtccataa aataccagcg tcaaattttc tatcttttgt
      421 accaatttaa aaatatctat cggcgcttca ccatcagctc ccaacttttt tctaagattt
      481 gaagctttat tacttaaatc cattcgtgat attcctttca cctttttcca ttttggcaat
      541 catgctttga ttcaaagaca aatataaaga caaatattca gcaatcttat cttgagtaag
      601 atttgaagat attctaagtt cttttattc
//
LOCUS       DAVLYR010000293          619 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.293, whole genome shotgun sequence.
ACCESSION   DAVLYR010000293 DAVLYR010000000
VERSION     DAVLYR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.293"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>619
                     /locus_tag="VYO63_002070"
     CDS             <1..>619
                     /locus_tag="VYO63_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1672895.1"
                     /translation="GINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDG
                     VEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVK
                     SLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSY
                     LSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGG"
BASE COUNT          217 a           81 c          125 g          196 t
ORIGIN      
        1 aaggaataaa tgttactatt aaaaaaggag aaactgttgc tattgttgga gaatcaggtt
       61 caggtaagag tacattagct aaaattttat taggtttatt agaacctaat attggttcaa
      121 tagaagttga tggagtagaa aaagaagaaa ttggtcaaac attgtataga aagatttttg
      181 gagcagtgtt acaaaattca accctaagtt atggtacctt aagagagaat ttgacatttg
      241 gacactttgt ttcagatgaa gaattaatga caaatctaaa ttcaattggt cttagcaatg
      301 tagttaaatc tttacctctt ggattagaga caatcatcgc tgaagaaggt aataactttt
      361 ctggagggca gcagcaaatg atacttttag ctcgttgtct tttgtcgaaa ccttcggtag
      421 ttgttttgga cgaagcaaca agtagtttag ataatttatc tcaacaaatt acaacttctt
      481 acttaagtga aatcggtacc actaagattt taattgccca tcgactagat actatcaagt
      541 ctgcagataa gatcttagta atgcataatg gtgaaattgt agagattggg acccatagag
      601 aacttcttga actaggagg
//
LOCUS       DAVLYR010000294          617 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.294, whole genome shotgun sequence.
ACCESSION   DAVLYR010000294 DAVLYR010000000
VERSION     DAVLYR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.294"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..612)
                     /locus_tag="VYO63_002071"
     CDS             complement(<1..612)
                     /locus_tag="VYO63_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1672896.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKK"
BASE COUNT          172 a          119 c          108 g          218 t
ORIGIN      
        1 ttttttatta gaattattta aagcgatgcg ttgagcctct ccctcttcct cgctaaaagt
       61 agctagagct ccgttttgaa taccattaca gctaacgaaa gctttagaaa attggagatt
      121 agagagattt tgtagggtca atgtgccaac aaaagcacct gtaatatcgc gataatttcc
      181 acctatcaaa atcaaatctg ttaattttcg ttcgcttaaa atcagaaaaa caggtagact
      241 gttggttacg acgcggatat tgtcaatagg caactcacgc gcaaaaaact ctaatgttgt
      301 tcctggtcca atgaaaatag tttctctttc ttctactaga ctgcctgcaa aatgggctat
      361 ttcttgtttt tctgccgttt ggagggcttg tttttcaata tttgatcgct cattagtcaa
      421 aagggagttg gttcgaagtt tttcagctcc accatgcaca cgaatcagca aatctttatc
      481 agctaattcc tgtaaatagc gccttgcagt catatctgaa acggctattt cgtccataat
      541 ctgtttaact gttatggttc ctttactatt tactatctct aaaattttgg ttaatttttc
      601 ttgtttgagc atattat
//
LOCUS       DAVLYR010000295          600 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.295, whole genome shotgun sequence.
ACCESSION   DAVLYR010000295 DAVLYR010000000
VERSION     DAVLYR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.295"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..>600)
                     /locus_tag="VYO63_002072"
     CDS             complement(<1..>600)
                     /locus_tag="VYO63_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1672897.1"
                     /translation="DYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFSNKL
                     KRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTANI
                     KNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDFFAERRSLNAEVVIKGVCLDPRIGN
                     FYNNPSFEFGGYCLPKDTKQLKKEFIEINAPVIEAIDIS"
BASE COUNT          207 a          109 c           73 g          211 t
ORIGIN      
        1 tttgaaatat caatcgcttc tatcactggg gcatttattt ctataaattc tttttttaat
       61 tgtttagtat ctttgggaag acaatatcct ccaaattcaa aagaaggatt attataaaaa
      121 tttccaattc ttggatctaa acaaacacct tttattacaa cttcagcatt taaacttctc
      181 ctctcagcaa aaaaatctag ttcattaaaa aaagcaacac ggagagctaa gaatgtgtta
      241 gaaaaaagct taattgcttc tgcttcagta ggagaaacta acataacatt tttaatattg
      301 gcagtactat gagtactaat cgaaaggaac aactctgcaa tttttcttcc ttcaactgtc
      361 tcatctccaa caactatgcg acttggatat aaattatcat atatagaaca accttctctc
      421 aaaaattcag ggacaaaaat gatatttttt gtatcaaaca gcctttttaa tttgtttgaa
      481 aagccgatcg gaactgttga ctttaaaata atctttccat taggttttac cctcagaatc
      541 ttcgataccg tttgttcgat ttcatatgta ttaaaactac caattttctc atcataatct
//
LOCUS       DAVLYR010000296          596 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.296, whole genome shotgun sequence.
ACCESSION   DAVLYR010000296 DAVLYR010000000
VERSION     DAVLYR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.296"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..337)
                     /locus_tag="VYO63_002073"
     CDS             complement(<1..337)
                     /locus_tag="VYO63_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672898.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLV"
     gene            complement(433..>596)
                     /locus_tag="VYO63_002074"
     CDS             complement(433..>596)
                     /locus_tag="VYO63_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672899.1"
                     /translation="TPVYSLGSGSRIVMYYNGGGDNYIGSGTRLAMAPQFGNHVRIHT
                     SGSWSPDSY"
BASE COUNT          226 a          120 c           96 g          154 t
ORIGIN      
        1 agactagaaa agaagaaata attgcagtac tacccacaat agctagatac ctctttttag
       61 aaattcggca caattgtgct gttagaaaat gactctcagc atcctctatt atctgactag
      121 aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat cctcgaatag
      181 aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg aaagcaagga
      241 aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaat cgaggtgatt
      301 gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct tataataaaa
      361 atagggtgta gcattctttt ttcatgctac acccacaatc aaccatcttt aaggcttact
      421 ctgacaagta agttaataag aatctggact ccaagaacct gaagtatgaa ttcttacatg
      481 atttccaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat tgtcaccacc
      541 tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg gggtaa
//
LOCUS       DAVLYR010000297          577 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.297, whole genome shotgun sequence.
ACCESSION   DAVLYR010000297 DAVLYR010000000
VERSION     DAVLYR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.297"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..154)
                     /locus_tag="VYO63_002075"
     CDS             complement(<1..154)
                     /locus_tag="VYO63_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1672900.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEISL
                     STFFELL"
     gene            complement(167..>577)
                     /locus_tag="VYO63_002076"
     CDS             complement(167..>577)
                     /locus_tag="VYO63_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1672901.1"
                     /translation="HHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLASLFKDE
                     VNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGISEIGS
                     LEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
BASE COUNT          193 a          114 c           63 g          207 t
ORIGIN      
        1 ataataattc aaaaaatgtt gaaagagaaa tttcattttc tcccctttca aatctagaaa
       61 gttgagattt tgatataaaa tcagctgcag catactctaa agaatatccg tttaattttc
      121 taaaatatcg aaaagcttct ccgtaatttt tcacactttc attcccttaa ctttcttttc
      181 gagcgataaa cataccatat tctttaggaa tgatcaacat accattatta aactcacttt
      241 ccaatctttt acgtattatt tcctcttcta gtgaacctat ttctgaaatt cctttaaacg
      301 attggatgta tttaactagg tcatctatac tagttacctg taactcatca tcatagagta
      361 atgtatcgac agagttaaaa tacctactca ggtacctttt accattttgt aaagtaaacg
      421 ttctattttc taaatcttga ttaacctcat ctttaaataa acttgccaaa taatttacaa
      481 ctccattttc accaaatgta gcacagtaaa aaatgcctcc ggtttttaag accctattca
      541 cctcagagag agctttagga atatcattca catgatg
//
LOCUS       DAVLYR010000298          574 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.298, whole genome shotgun sequence.
ACCESSION   DAVLYR010000298 DAVLYR010000000
VERSION     DAVLYR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.298"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            184..564
                     /locus_tag="VYO63_002077"
     CDS             184..564
                     /locus_tag="VYO63_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1672902.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
BASE COUNT          174 a           87 c          129 g          184 t
ORIGIN      
        1 actggctaat taatcagtta aacactagtt aaggagtaat gatgaaaaaa agaacaatac
       61 tattattgat gaccagtctg ttggctcttg tcttaggagc atatggtttc ttggacatat
      121 tgatcctgga tcattctcat caggattact ctttactgct attttagaaa ctggggtggt
      181 ttgatggaaa gtattggtat tggtcttgtt atcgtttcac attccaaaca cattgcagaa
      241 ggtgttgttg aactgattag taaagtagct aaagatgttc cgattactta tgtaggagga
      301 accgagggcg gaggaattgg aacgagtttt gatcaagtag atagggttgt ttccgaaaat
      361 ccagcagata ctttacttgc cttttttgac ctaggttctg ctaaaatgaa cttaaaaatg
      421 gtgactgatt tcagtgataa aagtatcatc atcaacaggg ttccaattgt agaaggtgcc
      481 tataatgcag ctgctcttct gcaggctggt gcagaactgt cagttattca aacacagtta
      541 gcggagcttg aaatcaataa ataaggaatt ttac
//
LOCUS       DAVLYR010000299          568 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.299, whole genome shotgun sequence.
ACCESSION   DAVLYR010000299 DAVLYR010000000
VERSION     DAVLYR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.299"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>568
                     /locus_tag="VYO63_002078"
     CDS             <1..>568
                     /locus_tag="VYO63_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1672903.1"
                     /translation="YNFSSDFQLIEGLITHLMTLQVRLESRITLNNPLVDEIKQNYSD
                     IFFMTREILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATGFGAA
                     QMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPVFKVSVF
                     LKSDEMEMIRKAMDQMQVSSYVQSSKINK"
BASE COUNT          199 a           69 c          112 g          188 t
ORIGIN      
        1 tataactttt cttcagattt ccaactaatt gaaggattga taactcatct aatgacacta
       61 caagtacgtt tagaaagccg gattacctta aataaccctt tggtagatga aattaaacag
      121 aattatagtg atatattctt tatgactaga gaaattcttg caaatatgga caagttcttg
      181 gagtggacaa tatcagatga tgaagtagca tatgtaagtc ttcacttttt agctgctatg
      241 gaacgtagca aagagagtac taagtttaat attcttgcta tttgtgcaac aggatttggt
      301 gcagcccaaa tgttaagaaa tcgtttggaa acggagtttg ggaagcgagt agaagttgtt
      361 gatgtaatag gctattatga attgaatcaa gaaaaactta aaggaataga ttttatagtg
      421 tcagctgttg atttatcaaa tctatatttt caaatcccag tttttaaagt tagtgtgttt
      481 cttaagagtg atgagatgga aatgattaga aaagctatgg atcagatgca agtttctagt
      541 tatgtacaat catcaaagat taacaaat
//
LOCUS       DAVLYR010000300          548 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.300, whole genome shotgun sequence.
ACCESSION   DAVLYR010000300 DAVLYR010000000
VERSION     DAVLYR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.300"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>548
                     /locus_tag="VYO63_002079"
     CDS             <1..>548
                     /locus_tag="VYO63_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV1672904.1"
                     /translation="NQFLPDRFVEGKCPNCGTHSRGDQCDNCSAILDPIDLVDKRCSI
                     CSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRWRKNAINLTKRYLREGLPDRAV
                     TRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDWAQKLQNNITDFWNNRTKSYYV
                     HGKDNIPFHTIIWPAILSGLEI"
BASE COUNT          187 a           80 c          110 g          171 t
ORIGIN      
        1 aatcaattct tacctgatag gttcgttgaa ggaaaatgcc cgaattgtgg aacccattca
       61 agaggagatc aatgtgataa ttgctcagca attttagatc ctatagattt agttgataaa
      121 agatgttcta tttgttccaa tgaaccagaa gttcgtgaga cagaacactt ttattatgtt
      181 tttagtgagt ttcaaaattt gttagagact tacttaaatg atgctgaaga aacagtgaga
      241 tggcgaaaaa atgcaataaa tctaactaaa aggtatttac gggaaggttt acctgatcga
      301 gctgttactc gtgatctacc aaatgggatt ccagttccca ttgatggttt tagagataaa
      361 aaaatatatg tctggtttga agcggtagca ggttactaca cagcaagtgt tgattgggcg
      421 caaaaacttc aaaataatat tactgatttt tggaacaatc ggacaaagtc atattatgtt
      481 catggaaaag ataacatccc atttcatact attatttggc cggcaatact ctcaggttta
      541 gaaataga
//
LOCUS       DAVLYR010000301          547 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.301, whole genome shotgun sequence.
ACCESSION   DAVLYR010000301 DAVLYR010000000
VERSION     DAVLYR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.301"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
BASE COUNT          239 a           80 c           93 g          135 t
ORIGIN      
        1 acaaaagatg aattttataa gttaataaaa acgttaaatg atgaagaaat aagattaaac
       61 ataatggaaa ttttagaaga aagaatagat gataatgtag aaaaatcatc taaagatgaa
      121 ataacataat tgtcccttgg ttacaagggc gcaattatac accctaaagg gtgcttgcgt
      181 cctgcggagt caaacccttg cagggagcaa caaccattcg ctttttaaaa gtcaagggta
      241 aataaactcg ctaaggctcg cccttgactt ttaaaatttg aaatgaaagt aacaattaag
      301 ccgacatact gaaacaacaa aaattaattc agtagtcggc ttttttaatt ctatcatttc
      361 aaaacgctca ttcacaaaga ggatataaaa aaactattaa gcctccacct aaaacaacaa
      421 agaaagaaag gaagttataa aaatggcaga cagttttcat ttttcagtaa acataatatc
      481 aagaggaaaa ggcaaaagtg cagtagcaag tgcagcatat ataagtggag aaaaaataaa
      541 aaatgaa
//
LOCUS       DAVLYR010000302          533 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.302, whole genome shotgun sequence.
ACCESSION   DAVLYR010000302 DAVLYR010000000
VERSION     DAVLYR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.302"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..208
                     /locus_tag="VYO63_002080"
     CDS             <1..208
                     /locus_tag="VYO63_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672905.1"
                     /translation="NQPATEVPSSGITVKLNAKDNAGNWTSASNKKEITVKIVSAKPT
                     YPDKILVKNPDNIKDTEKMPLLKN"
BASE COUNT          206 a           85 c           95 g          147 t
ORIGIN      
        1 taatcaacct gctactgaag ttcctagttc aggaattact gtaaaattaa atgctaaaga
       61 taatgctgga aactggacaa gtgcttcgaa taaaaaagaa ataacagtaa aaattgtttc
      121 tgctaaaccg acatatccag acaaaatctt agtgaaaaat cctgataata taaaagatac
      181 agaaaaaatg ccattattga aaaattgaaa gaggcaaata aaaatcatcc agcaggagct
      241 ccaacctttg ctaaaggtga aggagagcat gcaaatgata ttgtagcaac ttattcagat
      301 ggtacaactt attatgtacc gttaaatgat gtgacaaaat atgcgaggta gtggctgtac
      361 cactcactta ttcacctccc cgtgatttgt agtagtgata ggctttctca ctattattat
      421 aaaacaaaat aaagatcaca acactttttc attctgtgtt gtgccttgag tgaaacgaaa
      481 ggaatgaatt ataaatatga aaagtatagt cactagcata gatgagcgct tgc
//
LOCUS       DAVLYR010000303          508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.303, whole genome shotgun sequence.
ACCESSION   DAVLYR010000303 DAVLYR010000000
VERSION     DAVLYR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.303"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            50..508
                     /locus_tag="VYO63_002081"
                     /pseudo
     CDS             50..508
                     /locus_tag="VYO63_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
BASE COUNT          200 a           64 c           99 g          145 t
ORIGIN      
        1 aactatcgtc tttttctttg caaattttta agaagggagg tgatcttgca tggactttga
       61 atatttttat aacagagaag cggaaagatt taacttctta aaagtaccgg agatattagt
      121 tgatagagaa gaatttcggg gcttatcagc agaagcaatt atcctttatt ccatacttct
      181 taaacagaca ggaatgtcat ttaagaataa ctggatagac aaggaaggca gagtatttat
      241 ctattttact gtcgaagaaa ttatgaaaag aagaaatatc tcaaagccaa ctgccataaa
      301 aacattagat gagcttgatg taaaaaagga ataggactga tcgaaagagt aaggcttgga
      361 cttggtaagc cgaacatcat ttatgttaaa gactttatga gtatatttca ggtaaaagaa
      421 aatgacttac agaagtcaaa aaacttaact tcagaagtaa aagattttaa cctcagaagt
      481 aaagaaaatg aacttcaaga ggttaaga
//
LOCUS       DAVLYR010000304          502 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.304, whole genome shotgun sequence.
ACCESSION   DAVLYR010000304 DAVLYR010000000
VERSION     DAVLYR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.304"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..454)
                     /locus_tag="VYO63_002082"
     CDS             complement(<1..454)
                     /locus_tag="VYO63_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672906.1"
                     /translation="MVRDVIPINLVQEYCQGQPYGLLKKMLKRIRLSREISLLLATII
                     VYFFTHRILPLSVFTFIFSYILLFAQSYLGGNTAWIGNRRLIIDDEFEKILLSKSYIK
                     EISSARYSEYLTCEYKNFTPIILLAIFENLLDSYLLQNQSEVDLDIFYK"
BASE COUNT          175 a           82 c           79 g          166 t
ORIGIN      
        1 ctttataaaa aatatctaag tcaacctcac tctgattctg aagcaagtag gagtcaagca
       61 aattttcaaa aatagctaac aatatgattg gtgtaaagtt tttgtattca catgtcaagt
      121 attctgagta tcgtgcagat gatatttctt ttatataact ttttgaaagc aagatttttt
      181 caaattcatc atctattatt aatcttctat ttccaatcca agcggtatta ccacccaaat
      241 aagattgtgc aaatagcaat atgtaagaaa atatgaacgt aaatactgaa agaggtagaa
      301 ttctatgcgt aaaaaaatag acgataatgg tagccaggag taaagatatc tctcgtgaca
      361 atcgaattct tttcagcatc ttttttaaca gtccgtaagg ttgtccttgg caatattctt
      421 gaactaaatt aatcggaatg acatctctca ccatttctgg aaagtagagt aagtttatag
      481 gttgaaatct ccagctccca tc
//
LOCUS       DAVLYR010000305          496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.305, whole genome shotgun sequence.
ACCESSION   DAVLYR010000305 DAVLYR010000000
VERSION     DAVLYR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.305"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            337..>496
                     /locus_tag="VYO63_002083"
     CDS             337..>496
                     /locus_tag="VYO63_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672907.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT          189 a           74 c          103 g          130 t
ORIGIN      
        1 ttgaacctta acaattgaat agaccaagac aagacgttaa ttatttttgg agcgtaataa
       61 cctatccgtc aagatctttc ggctgaataa ttcggcaaaa aaagttctgc taagcaaaaa
      121 gaaagtgagc gataagtaag agtttgaata tagggaggga taccctatcc gctatgaaga
      181 aaatgaggat aatgatactt ctaaggttga agccggctca tccggaatag aggcggaggt
      241 gagattccta tgttgctaat ccaaagcact tgtcaatgtc aaaaaactta attaaacaaa
      301 taattattag atataaaact ttagaggagg tatatattgg acaatgactg gaatggattg
      361 gctgatttaa tagcaaatct gattgcaaaa tatgctggtg ctttagattt agataatcta
      421 cccgatccaa cgccagcaaa aaatcaagag atgaaaaata gttttgacat ggcaaaaaca
      481 caaattgaga cggact
//
LOCUS       DAVLYR010000306          494 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.306, whole genome shotgun sequence.
ACCESSION   DAVLYR010000306 DAVLYR010000000
VERSION     DAVLYR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.306"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(113..487)
                     /locus_tag="VYO63_002084"
     CDS             complement(113..487)
                     /locus_tag="VYO63_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1672908.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          167 a          112 c           63 g          152 t
ORIGIN      
        1 tttttatttc ttcgctaaca aaatcatagg ctaaaaaagt tatggatgat ataaatagac
       61 atacaaaaaa aataataaca aaaatttttt ccattttaat acccatatct agttaccaca
      121 aggcgtttaa atttgggtct ctcctattta catcattgtt ggcccagatt gttgtaatag
      181 catcgccaat tgacatccaa gctgcaaatc cattaatagc agacagtgca aatcctgcca
      241 ctctatttgc aactcgtacc ccaacatatc ttgcagctgc tccagcaatt gcgcttgtaa
      301 ctcgccgcaa accataatta cgaatataat ttctaactag actgattgct cctccaccta
      361 aaacaccatt aaaagctata ttaatggctg ttgcaacagg ccatctcaga atagaaaatc
      421 ccccatcaag atacatcatc tcttcttgct caagagcaac ataattattt ggtaatacga
      481 gttccattta tttt
//
LOCUS       DAVLYR010000307          491 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.307, whole genome shotgun sequence.
ACCESSION   DAVLYR010000307 DAVLYR010000000
VERSION     DAVLYR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.307"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            8..>491
                     /locus_tag="VYO63_002085"
     CDS             8..>491
                     /locus_tag="VYO63_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1672909.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPSVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTATKYVPLETKLLGFGLSIPGHYNKDSGSI
                     I"
BASE COUNT          182 a           94 c           77 g          138 t
ORIGIN      
        1 gaaacaaatg attacaaaac aatcagcctt tttacaaaat agagccacta tactggaatt
       61 tctatatcga aatccagcaa catcccgtac agatattgtt aatgaaacag gacttactcc
      121 agcaacaacg actaacatca taaaagagct aagcgaacaa tccctcatat acgaaactgg
      181 tgatgaattt agcgagtttt ccggatctgg aagacgtaga aaaactattt ctattacaga
      241 taatatccca tctgttgttg gaggtattga aataaacgtc ttaggtatct ttctcagtct
      301 atgtgactta caagggaaaa ctcttttcga gacagaaatt ttgaatgaag attatcctat
      361 ttcagaaatc aattccacca ttaccaatat gataaaaaca gctacaaagt atgtcccttt
      421 ggagacaaaa ttacttggat ttggcttatc aatacctgga cattataaca aagactccgg
      481 aagcatcatt a
//
LOCUS       DAVLYR010000308          478 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.308, whole genome shotgun sequence.
ACCESSION   DAVLYR010000308 DAVLYR010000000
VERSION     DAVLYR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.308"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            102..401
                     /locus_tag="VYO63_002086"
     CDS             102..401
                     /locus_tag="VYO63_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1672910.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          170 a           93 c           94 g          121 t
ORIGIN      
        1 ggcttgatgg ctatgctact aataacaatt aggagagaaa atcaggcact tgttaacaac
       61 aaggattatc cccttgagat gaaaggaact ttagaaatct tatgatgaac atgcaaaaca
      121 tgatgcgtca agcacaaaaa cttcaaaaac aaatggaaca aagccaagct gaacttgctg
      181 ctatgcaatt tgttggcaaa tctgctcaag atcttgtcca agcgacctta actggcgata
      241 agaaagttgt cagcattgat ttcaatccag ctgtcgttga cccagaggac cttgagactc
      301 tttctgatat gaccgttcaa gccatcaact ctgctcttga acaaatcgat gaaactacca
      361 agaaaaaact gggtgctttc gctgggaaat tacctttcta aaaacaagga gctagaacaa
      421 tgcttgtcga taacaaaggc taagaaaggt gcaaaaatga ctctataata tttgtagt
//
LOCUS       DAVLYR010000309          460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.309, whole genome shotgun sequence.
ACCESSION   DAVLYR010000309 DAVLYR010000000
VERSION     DAVLYR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.309"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>460
                     /locus_tag="VYO63_002087"
     CDS             <1..>460
                     /locus_tag="VYO63_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672911.1"
                     /translation="NKEKLYRSILVYDAFKFGTDKDKDKVTHQATFETDHPAIKYFFG
                     PAGNNVVHNGHGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRSG
                     LGPEFYAKGLLQAPDHPYDPTITINSVLKYEDSENSTRLQVADPTQRFNSA"
BASE COUNT          172 a           71 c           87 g          130 t
ORIGIN      
        1 aataaagaaa aattatatag aagtattcta gtatatgatg ctttcaaatt tggtactgac
       61 aaagataaag acaaagtaac tcatcaagct acttttgaaa ctgatcatcc agctataaaa
      121 tacttctttg gacctgcggg aaataatgtt gttcataatg gacacggagc atatgctacc
      181 ggagatgcat tctattatat ggcatatcgt atgttggata aagatggagc tgtaacatat
      241 actcatgaaa tgacacataa ctcagataga gagatttatc ttggaggata tggaagaaga
      301 agcggattag gacctgagtt ttatgcaaaa ggattattac aagctccgga tcatccatat
      361 gatccaacaa taacaattaa ctctgtcttg aaatatgagg attcagaaaa ttcaacaaga
      421 cttcaagtag ctgatccaac tcaacgattt aatagtgcag
//
LOCUS       DAVLYR010000310          455 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.310, whole genome shotgun sequence.
ACCESSION   DAVLYR010000310 DAVLYR010000000
VERSION     DAVLYR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.310"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..259)
                     /locus_tag="VYO63_002088"
     CDS             complement(<1..259)
                     /locus_tag="VYO63_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672912.1"
                     /translation="MFYNQQKNRMIYVLSNFLYAISVSIIYAWNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGI"
BASE COUNT          187 a           78 c           68 g          122 t
ORIGIN      
        1 aaataccaaa taccaagcca cttttcaatt tgtataaata gaaaatgtag gataatagaa
       61 aataaaacag gactaacaaa attgtattga ctacaatagc tactataaaa tctaatccta
      121 aatcacctgg aatacccaat ttacttacta tgactagtaa cacaatgcca ttccaagcat
      181 aaataatgga aactgagatg gcatatagaa aattagacaa gacgtaaatc atccgattct
      241 tctgttgatt ataaaacaaa cttaccgtat tgtgggaaaa atctctacta atgactcgat
      301 tagcatgtat aacagccatt aacataccga gcatggcata aaagtttgaa atatgcttaa
      361 taccaagcga tgtcccttga gtctttagaa taaagaatgt aagcaatatc ggcggtataa
      421 acgagataag caaactgaga tatattacgc ctgat
//
LOCUS       DAVLYR010000311          453 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.311, whole genome shotgun sequence.
ACCESSION   DAVLYR010000311 DAVLYR010000000
VERSION     DAVLYR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.311"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>453
                     /locus_tag="VYO63_002089"
     CDS             <1..>453
                     /locus_tag="VYO63_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1672913.1"
                     /translation="IKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCL
                     IGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEK
                     AKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPS"
BASE COUNT          175 a           58 c           89 g          131 t
ORIGIN      
        1 agataaaaaa tcttagctac aaatttactg atgatgagtg tttaagctta aaagatattt
       61 cgttcaagct tgggaaaatt tatggcataa taggatccaa cggacgagga aaatcaacgc
      121 ttttaagatg tttaataggt cttgagaaaa aatcaaaaga agaaatttat tttaagggag
      181 agaagctatc taaaaaagaa agactcaaaa actcttcact tgttatgcaa gatgtaaatc
      241 atcaattatt cacagatgaa gtattcaacg agcttagatt aggagtaaag aattttgatg
      301 aagaaaaggc gaaaatcatt ttaaaagatt taggcctgga cgaatttatt gaaaggcatc
      361 cgatgagttt atcaggaggg caaaagcaaa ggcttgcaat agcatctgtt atgtgcaaga
      421 attctccatt tgtctttttt gacgaacctt caa
//
LOCUS       DAVLYR010000312          452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.312, whole genome shotgun sequence.
ACCESSION   DAVLYR010000312 DAVLYR010000000
VERSION     DAVLYR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.312"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..452
                     /locus_tag="VYO63_002090"
                     /pseudo
     CDS             1..452
                     /locus_tag="VYO63_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          166 a           90 c           73 g          123 t
ORIGIN      
        1 tcttcttcta aaagccatta aactagctcg ttcgacctac tactatcact tgaaacagct
       61 agacaaaaca gataaggacc aagagcttaa agctgaaatt caatccatct ttatcgaaca
      121 caagggaaat tatgcttatc gtcggattta tttagaacta agaaatcgtg gttatctggt
      181 aaatcataaa agagttcaac acttgatgaa gtactcaatt tacaagctaa aacgcgacag
      241 aaacgaaaat attcttctca taaaggagac gttggcaaga aagcagagaa tctcattcaa
      301 ggccaatttg aaggctctaa aacaatggaa cagtgctaca cagatgtgac tgaatttgcc
      361 attccagtaa gtactcaaaa gctttactta tcaccagttt tagatggctt taatagcgaa
      421 attatcgcct ataatctttc aacttcaccc aa
//
LOCUS       DAVLYR010000313          448 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.313, whole genome shotgun sequence.
ACCESSION   DAVLYR010000313 DAVLYR010000000
VERSION     DAVLYR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.313"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(24..176)
                     /locus_tag="VYO63_002091"
     CDS             complement(24..176)
                     /locus_tag="VYO63_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672914.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            complement(307..>448)
                     /locus_tag="VYO63_002092"
     CDS             complement(307..>448)
                     /locus_tag="VYO63_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1672915.1"
                     /translation="YLHCSQSAYSRIESGKQDIPTSFLKQLAKLYQVTTDYILEMDKF
                     SC"
BASE COUNT          132 a           87 c           72 g          157 t
ORIGIN      
        1 tgatagttgc atagtatact ttattactga ttgttccaaa ctttttcctc cttgtaacat
       61 gtataatttc ctccgtgggt atgactggtt aaatcattca tgggcatttc acccgctgtc
      121 ctttttcgct ggcagcctga acgttcagaa gtatcattat ccttatttgt agtcatggca
      181 gatagagaac cactctattt tatagctaat aattatcgct cctatcacgg cgtatcaagc
      241 taaagtgctc gacaaataag aaagtcccac ctcggtttat tcagttttca aagggcttag
      301 gatttattaa caactaaact tatccatctc taaaatataa tctgtagtta cctgatataa
      361 tttagctaat tgtttcaaaa atgatgtagg aatatcttgc ttgccacttt ctattctaga
      421 ataagcagac tgtgaacaat gaagatac
//
LOCUS       DAVLYR010000314          442 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.314, whole genome shotgun sequence.
ACCESSION   DAVLYR010000314 DAVLYR010000000
VERSION     DAVLYR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.314"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..442)
                     /locus_tag="VYO63_002093"
                     /pseudo
     CDS             complement(1..442)
                     /locus_tag="VYO63_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          123 a           75 c           83 g          161 t
ORIGIN      
        1 ttaggcgaac aagaaagatt ataggcgata atttcgctgt taaagccatc taaaactggt
       61 gataagtaaa gcttttgagt acttgctgga atggcaaatt ctgtcacatc tgtgtagcac
      121 tgttccattg ttttagagcc ttcaaattgg ccttgaatga gattctctgc cttcttacca
      181 acgtctcctt tattttcgtt tctgtcgcat tttagcttgt aaattgagta ctttcatcaa
      241 gccttgaact cttttatgat ttaccaaata agcacgattt cttagttcta aatgaataca
      301 gcgataagca taatttccct tgtgttcgat aaaaatggat tgaatttcag ctttaagctc
      361 ttggtcctta tctgttttgt ctagctgttt caagtgatag tagtaggtcg aacaagctag
      421 tttaatggat tttagaagaa ga
//
LOCUS       DAVLYR010000315          433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.315, whole genome shotgun sequence.
ACCESSION   DAVLYR010000315 DAVLYR010000000
VERSION     DAVLYR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.315"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            38..>433
                     /locus_tag="VYO63_002094"
     CDS             38..>433
                     /locus_tag="VYO63_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1672916.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKV"
BASE COUNT          118 a           58 c           89 g          168 t
ORIGIN      
        1 gggggctttt tagttcacaa cttataggag gtgtactatg aaaatcttaa aacgttacat
       61 attggaactc tgttttattt taagttttgc tttacctttt ataaaaggag ccaatgcaga
      121 taatggtaga tgctttgtgg aaacctatta cggttttact tttttgatgg aacatgctat
      181 tgtaacagct gtctttatct attcgttttt aattgctttc ttactaaaaa aacgatgggc
      241 gaaatggatt gctgctggta gttattgctt tttagtccta tggattgcga cagagggtta
      301 tttcttccgc atgtcactag aagatttgat acgactttgg acaagtttgg aaatcctgac
      361 acaaacgtat cagttgggct tttatctaaa cattttgttg ggaattttgt tgataataaa
      421 gtattttaag gtt
//
LOCUS       DAVLYR010000316          414 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.316, whole genome shotgun sequence.
ACCESSION   DAVLYR010000316 DAVLYR010000000
VERSION     DAVLYR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.316"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..411)
                     /locus_tag="VYO63_002095"
     CDS             complement(<1..411)
                     /locus_tag="VYO63_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1672917.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYV"
BASE COUNT          128 a           74 c           65 g          147 t
ORIGIN      
        1 cacatattga attaaatcaa taaatttttc atgatcaaca aaggcagtag tttcaatggc
       61 agtgtgtatg tgatgttctt tagctgattt taagatggct ttagcaaatt caaactgagc
      121 aaatatttca cctcctgata aagttaaacc tccaccggat tcttcgtaaa attctttgtc
      181 ttttaatacc tctgtaataa tttcttctac agtcttttct tcaccgacta aggtgaattt
      241 ctctcgttga gcatctttca ttttttcagg tttcattctt tgagattcag gattagaaca
      301 ccatggacag cgcagaggac atccttttaa aaaaacagtt gtacgaatac ccgggccgtc
      361 atgaattgaa aagtgttgaa tattaaaaat aattccttta gatatttcca taag
//
LOCUS       DAVLYR010000317          405 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.317, whole genome shotgun sequence.
ACCESSION   DAVLYR010000317 DAVLYR010000000
VERSION     DAVLYR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.317"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
BASE COUNT          157 a           82 c           44 g          122 t
ORIGIN      
        1 agacatgctt agatataata aaaataacaa aaaatgaatt tcaccaattc ctgcaaaaaa
       61 atgtttcata gctatagttt tacgcccata aatacccatc aaatttaccg tctacgatat
      121 ctaaaccata tgcaatggct cctcctaatg agaagacttc agcaacagta gccacaatat
      181 taagagcatt agtaatagtt gccatactaa ctccaccaat atatcttgtt atagcactta
      241 gttacctaga taagatatcc tttagcttgc ttccaaaatt taagtctaca cctataatat
      301 gagttcatat tagtactgct aaatcctatc tatagtaatg tcaaacacct aaataaagtc
      361 atctctccaa ttcattcaag aaatgaataa aggcaaaaaa tcttt
//
LOCUS       DAVLYR010000318          402 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.318, whole genome shotgun sequence.
ACCESSION   DAVLYR010000318 DAVLYR010000000
VERSION     DAVLYR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.318"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
BASE COUNT          136 a           85 c           45 g          136 t
ORIGIN      
        1 atgtccaatt ttataaatac tgggctgaca caaaatatcg tgcttataag cagtaaaatt
       61 attaaaattg tgtcaaaaaa ataacgctta tttttcatta gtctactttc cattgttaaa
      121 taatactact gctaacacca agccaactta gcacattgca tcacctaatc ctgcgctact
      181 tgtccataat cctttaatgt ttctactaaa attattccaa tctcccccat caagatagct
      241 tatttatcct agacaacgcc tactgcaaca gtataaataa gattcatcag ccatcttttc
      301 tggcaatcat atgggtagac taatgatatt tctagttact caaaaatatc tagctatact
      361 agagttattt ctaatcccta ggacacttgt taatccaaat gt
//
LOCUS       DAVLYR010000319          401 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.319, whole genome shotgun sequence.
ACCESSION   DAVLYR010000319 DAVLYR010000000
VERSION     DAVLYR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.319"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            85..399
                     /locus_tag="VYO63_002096"
     CDS             85..399
                     /locus_tag="VYO63_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV1672918.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
BASE COUNT          146 a           58 c           79 g          118 t
ORIGIN      
        1 acgctttatt ttataataaa aatagataac agataagtca acttagcaca gctttgattg
       61 gatttaagaa aaggagaaaa tattatggca aaagctttaa ttatttgtgc agcaggaatg
      121 tcttcatcac ttatggcaca gaaaacaaca gagtttctaa aaggaaaagg gaaagatatt
      181 gtagttgatg ctattagtgc tactgaagga gggaaagcta tcgcagcagc agcttttgat
      241 ttgtatcttg tcagccctca aacaaaaatg tactttaagc aatttgaaga ggctggttct
      301 aaagcaggaa aaccagttgt acaaatacct cctcaagcct atattcctat tccaatggga
      361 attgaaaaaa tggctaattt gattcttgag aatatttgat g
//
LOCUS       DAVLYR010000320          400 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.320, whole genome shotgun sequence.
ACCESSION   DAVLYR010000320 DAVLYR010000000
VERSION     DAVLYR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.320"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            186..>400
                     /gene="blpU"
                     /locus_tag="VYO63_002097"
     CDS             186..>400
                     /gene="blpU"
                     /locus_tag="VYO63_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1672919.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAA"
BASE COUNT          148 a           49 c           97 g          106 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaacg atgtcacaat ttgaaattat ggatactgag atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagtacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgcgggagg agctatactt ggaggtgtgg cctatgcagc
//
LOCUS       DAVLYR010000321          379 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.321, whole genome shotgun sequence.
ACCESSION   DAVLYR010000321 DAVLYR010000000
VERSION     DAVLYR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.321"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            4..>379
                     /locus_tag="VYO63_002098"
     CDS             4..>379
                     /locus_tag="VYO63_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1672920.1"
                     /translation="MPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFV
                     GNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVRE
                     FLVNYYAHSKENGITGVTQKTIN"
BASE COUNT          135 a           44 c           80 g          120 t
ORIGIN      
        1 tatatgccag atcgaatttt aaagttgatg gcttgtttta aaaagagtag aatgaagaat
       61 ttagctttag tttatagtta tggaataata atttatccaa atggtacacg agaagaggag
      121 aagaccgatt ttgttggaaa tcccttgttt gttcaaatgg ttcacaatat agcaggtacg
      181 tcattttggt tgtgtaaaaa agaggtgcta gaattaatta atggttttga gaaaatagat
      241 tcacatcagg acggtgttgt tttattaaaa ctacttgctc aaggatacca aattgatata
      301 gtgcgagaat tcttggtgaa ttactacgct cacagtaaag aaaacggtat cactggagtg
      361 acacaaaaaa caattaatg
//
LOCUS       DAVLYR010000322          369 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.322, whole genome shotgun sequence.
ACCESSION   DAVLYR010000322 DAVLYR010000000
VERSION     DAVLYR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.322"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
BASE COUNT          126 a           72 c           46 g          125 t
ORIGIN      
        1 aatactgtat tattaccctc ttaaggtgcg aaattattca agttcttaaa aaatgcttct
       61 ctatttccgt aagatagctt atttcttcga tttaagaact gaagatgata gttctaacct
      121 ttagaaatcc aagccttcac tagctcaaaa actcctatct ttcttcctca ataaatcatc
      181 tgagagatag gtattcctta tgtccttcaa attcatcact cctttggata ttgaggtgct
      241 agcctagaag cagacacccg attatctaaa ccatccttaa taaagatgac aagacagata
      301 agctcagtta attacttata tatcttttta atatggtata tttgtcaaca aaaagaaatc
      361 aaatcatac
//
LOCUS       DAVLYR010000323          358 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.323, whole genome shotgun sequence.
ACCESSION   DAVLYR010000323 DAVLYR010000000
VERSION     DAVLYR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.323"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..271
                     /locus_tag="VYO63_002099"
                     /pseudo
     CDS             1..271
                     /locus_tag="VYO63_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          111 a           61 c           71 g          115 t
ORIGIN      
        1 ggaagaagat atcagagaat ttctttggtt gcaggtctaa caaatgaatg gtgagttaat
       61 cgctccaatg acttacgaag agacgatgac gagcgacttt tttgaagctt ggtttcagaa
      121 gtttctctta ccaacattaa ccacaccatc agttattatt atggataatg caagattcca
      181 tagaatgggt aagttagaac tcttgtgtga agagtttggg tataaacttt tacctcttcc
      241 tccctactca cctgagtaca atcctattga ggctctataa tatttgtagt gggtaaatcc
      301 cctatggata ttatggagcc tatttttgtg tagaaaaaaa gtcccataag atctataa
//
LOCUS       DAVLYR010000324          352 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.324, whole genome shotgun sequence.
ACCESSION   DAVLYR010000324 DAVLYR010000000
VERSION     DAVLYR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.324"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
BASE COUNT          125 a           41 c           75 g          111 t
ORIGIN      
        1 gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa
       61 tggttattat aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctttttacat
      121 cagctcagct ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt
      181 tcatttacta gaaatgaaac tgatgagaga tatcagtaga catctgagtc aggatattat
      241 ggaaaatgat aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg
      301 ttaggatttt agcgactagt tagctgggaa aggaagatat ttgtgacaaa ta
//
LOCUS       DAVLYR010000325          334 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.325, whole genome shotgun sequence.
ACCESSION   DAVLYR010000325 DAVLYR010000000
VERSION     DAVLYR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.325"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>334
                     /locus_tag="VYO63_002100"
     CDS             <1..>334
                     /locus_tag="VYO63_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEV1672921.1"
                     /translation="ETYKPNVLHFCELLAEEEGIKLSDTTVRKILYKKNILSPKSHRK
                     TKKRVRKQAKLNLNQPLDNPILPTAKDFLEDPKKVHPSRPRKKFAGELIQMDASPHAW
                     FGPETTNL"
BASE COUNT          130 a           76 c           55 g           73 t
ORIGIN      
        1 atgaaacata taaaccaaat gtccttcatt tctgtgaatt actagctgaa gaagagggaa
       61 ttaagctctc tgatacaact gttagaaaaa tactctataa gaaaaacatc ctttctccta
      121 agtctcacag aaagacaaag aagagagtaa gaaaacaagc taaactgaac ctgaatcaac
      181 ccctagacaa tccaatctta ccgacggcta aagacttcct ggaagaccct aaaaaggtac
      241 atcctagtag acccagaaag aaatttgctg gagaactcat tcaaatggat gccagccctc
      301 atgcctggtt tggaccagaa accaccaact taca
//
LOCUS       DAVLYR010000326          323 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065079
            SAMN33607063-rid17952243.denovo.326, whole genome shotgun sequence.
ACCESSION   DAVLYR010000326 DAVLYR010000000
VERSION     DAVLYR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607063
            Sequence Read Archive: SRR23725507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721384.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721384.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:11:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,929
            CDSs (with protein)               :: 1,929
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 65 of 122
            Pseudo Genes (incomplete)         :: 52 of 122
            Pseudo Genes (internal stop)      :: 33 of 122
            Pseudo Genes (multiple problems)  :: 25 of 122
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607063-rid17952243.denovo.326"
                     /strain="AUSMDU00065079"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..93)
                     /locus_tag="VYO63_002101"
                     /pseudo
     CDS             complement(1..93)
                     /locus_tag="VYO63_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          105 a           70 c           48 g          100 t
ORIGIN      
        1 gtagggagga aaaggtaaaa gtttatgccc aaactcttca cataagaact ctagcttacc
       61 cattctatgg aatcttgcat tatccattaa aacttccaga ctcataccct tctaaaatct
      121 attcaagcca cgtcagcttc acattatata tgttggtgac ctcacaagtt ttatctgcaa
      181 cctcaaagct gttctttgag cagcctacag ctagcttcct agtttaatct ttgcttttca
      241 ctgagtatca agttaaaaaa atgaggctgg tcaaaaagtc tttaaaatta taaaacgcat
      301 catatcaggt gtacatgaac tac
//
